LOCUS       DAWKOG010000001        54825 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.001, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000001 DAWKOG010000000
VERSION     DAWKOG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 54825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54825
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.001"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            52..1005
                     /locus_tag="WJK66_000001"
     CDS             52..1005
                     /locus_tag="WJK66_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEZ7577784.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHGVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1116..1439)
                     /locus_tag="WJK66_000002"
     CDS             complement(1116..1439)
                     /locus_tag="WJK66_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7577785.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGSN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1612..2973)
                     /locus_tag="WJK66_000003"
     CDS             complement(1612..2973)
                     /locus_tag="WJK66_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEZ7577786.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTVIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAIRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGDAAERYQEDPVRILRAIRLSGKLGFELSEETTAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPAPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASTEQRSEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGT
                     DWE"
     gene            complement(3270..4970)
                     /gene="recJ"
                     /locus_tag="WJK66_000004"
     CDS             complement(3270..4970)
                     /gene="recJ"
                     /locus_tag="WJK66_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEZ7577787.1"
                     /translation="MSVKIQTRSVNTDVFNHLLTAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQSLTNCEAAARRLADAVERQEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAAQGVDLLITVDNGIASIAGVARAQELGLDVIVTD
                     HHLPADTVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLTALRAELRRRDYFSDGIKEP
                     NLGDLLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDSEAQELAAQLNNLNIERREIEQSM
                     LQDALNAFPETLPSGQMTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAVRE
                     MVCEDDLSQTFITDGSLPACDITLEQAQNLARHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKVWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAA
                     "
     gene            complement(5039..5752)
                     /locus_tag="WJK66_000005"
     CDS             complement(5039..5752)
                     /locus_tag="WJK66_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7577788.1"
                     /translation="MLFRKTTAAVLAATLMLNGCTFMLWGMNDPFSQTTDRKHVDKDQ
                     IRAFGVVAEDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKQFQMVEPNPR
                     FAYQALPVKLESPASQNFSTEGLCLRYDTDRPADIAKLKQLEFEAVELDNRTIYTRCV
                     SAKGKYYATPQKLNADYHFEQSVPADIYYTVTKKHTDKSKLFENIAYTPTTLILDAVG
                     AVLALPVAALIAATNSSDK"
     gene            complement(5799..6311)
                     /locus_tag="WJK66_000006"
     CDS             complement(5799..6311)
                     /locus_tag="WJK66_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HEZ7577789.1"
                     /translation="MKMFEQEDEWISKTQMKKQMNDLQDLGMALTKLSNDTLKKIGLD
                     ADLYEAVTAYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQR
                     VEQARVRLLADDGALTQFMSDFPHADAGKLRTLIRNTKKEQEQNKPPKNFRALFQELK
                     TVMENGDAEI"
     gene            6458..7789
                     /gene="pmbA"
                     /locus_tag="WJK66_000007"
     CDS             6458..7789
                     /gene="pmbA"
                     /locus_tag="WJK66_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEZ7577790.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESIGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADPELMAQHIGDPDLYHEWDLDTEAAVGLAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTISGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(8086..8289)
                     /locus_tag="WJK66_000008"
     CDS             complement(8086..8289)
                     /locus_tag="WJK66_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEZ7577791.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            8532..8834
                     /gene="clpS"
                     /locus_tag="WJK66_000009"
     CDS             8532..8834
                     /gene="clpS"
                     /locus_tag="WJK66_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEZ7577792.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            8836..11115
                     /gene="clpA"
                     /locus_tag="WJK66_000010"
     CDS             8836..11115
                     /gene="clpA"
                     /locus_tag="WJK66_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003746132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEZ7577793.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVPNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDDEDGNDSDGLGREG
                     EEAEQKTGSLSDYTVNLNTEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLVGEAG
                     VGKTALAEGLAHQIVNGGIPDALKDAEVYALDMGSLLAGTKYRGDFEARVKSVLKQLE
                     KIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATTYDEYRTIFDK
                     DYALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAAAELSARYINE
                     RFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTVSHDDKQVL
                     QFLGRDLKNMVYGQENAIDALVAAVKMSRSGLGLPDKPIGSFLFSGPTGVGKTEAAKQ
                     LAYSMGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVLLLDE
                     IEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPGLGFTAKR
                     ERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIAKVVDKFLLRLEHRLLDKKVEAE
                     FTPALRKYLARKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLSNGGFVRIDWDAAK
                     EEAVLKFKKSKVKPETETV"
     gene            complement(11162..13033)
                     /locus_tag="WJK66_000011"
     CDS             complement(11162..13033)
                     /locus_tag="WJK66_000011"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEZ7577794.1"
                     /translation="MPPLAIRRSAKNLKKKFKKFCQFGFTGTPIFPENALGAETTAGV
                     FGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKKLSAAENRQALLHPE
                     RIREITQYILNQFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYEAFKTQQAGSLHPLK
                     VATIFSFAANEEQNAVGEIVDETFEPEAMDSSAKEFLQAAINDYNACFKTNFGTDSKA
                     FQNYYRDLAKRVKNQEVDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAFSRTNR
                     IYDATKTFGNIVCFRDLEQATIDAITLFGDKNTKNVVLEKSYEEYMNGYIDDRTGEVR
                     RGYLDVAKELRERFPDPDKIETEKDKKDFAKLFGEYLRAENVLQNYDEFAALRELQSV
                     DAADEDAMKAFQEKYYLSDEDVQEMRQVPMPSERAVQDYRSAYNDIRDWLRRQKAGEQ
                     KEQSKIDWDDVVFEVDLLKSQEINLDYILQLVFEHHKKIKGKAELVEEIRRIIRASIG
                     HRAKESLIVDFINDTDLDKVPDVPAILETFYTYAQEVMRREAAELIAAEDLNETAAKR
                     YLTGSLKRGYASENGTELTETLPKMSPLNPQYLTKKQSVFQKIAAFVEKFAGIGTDI"
     gene            complement(12994..13365)
                     /locus_tag="WJK66_000012"
                     /pseudo
     CDS             complement(12994..13365)
                     /locus_tag="WJK66_000012"
                     /inference="COORDINATES: protein motif:HMM:NF037510.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            complement(13384..14259)
                     /locus_tag="WJK66_000013"
     CDS             complement(13384..14259)
                     /locus_tag="WJK66_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ7577795.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENQLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPSENITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQIINQFEQTGTYANRYDVTVLVNGLP
                     LVQIELKKRGVAVREAFNQVHRYSKESFNSENSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLGVLLHYSVFDANDTLLIMRPYQ
                     IAAAERILWKINSSA"
     gene            complement(14338..15558)
                     /locus_tag="WJK66_000014"
     CDS             complement(14338..15558)
                     /locus_tag="WJK66_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ7577796.1"
                     /translation="MDMQSKAKKLIEMIQTAPVEWKPLGGENGIAIIKTGQAVSKQKI
                     SNNIGSYPVINSGKEPLGYIDEWNTENDPIGITTRGAGVGSITWQEGRYFRGNLNYAV
                     TIKNRTELDVRFLYHILLEFEQEIHALCTFTGIPALNASNLKKLLIPIPPLETQQKIV
                     KILDKFTELEAELALRKRQYRYYRDFLLDFDNQIGGGIADGYKGRLKDVVWKTLGEVF
                     DLKNGYTPSKSNKEYWENGSIPWFRMEDIRENSRILDNSLKHISKSAVKGGKLFPAKS
                     IMMSTTATIGEHALIKVNYISNQQLTNFTIKDEFKDALDINFAFYYFFIIAEQSKKLI
                     NTSSLPIISMKELKKLKIPIPPLPEQEKIAAILDKFDTLTHSISEGLPHEIALRRKQY
                     EYYREQLLAFPKAT"
     gene            complement(15724..16734)
                     /locus_tag="WJK66_000015"
     CDS             complement(15724..16734)
                     /locus_tag="WJK66_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HEZ7577797.1"
                     /translation="MSIILYTANDGTAQFALQEFGGQLWLTQADMAELYQTTKQNISK
                     HIKTILAEQELEEKATVNFQLTVQNENGRKVNRKIAHYSLPMIIAVGYRVRSARGIQF
                     RQWATERLDEYLTKGFAIDDERLKGTGGGDYWKELLNRIRDIRSSEKALYRQVLDLYA
                     TSQDYNPKSSESQTFFAAVQNKLHYAASRQTAAELIYSRADSSKDFMGLTTFQGAIPT
                     LNEAKIAKNYLTEDELFRLNRLVSAFFDLAEIKAQEQSPMYMRDWIAELDKFSGLYGQ
                     GTLQGAGSISRKQAEQKAEREYRAYEARILSPVEQAYLESVKALEKTAVQQIKQKKDR
                     TK"
     gene            complement(16731..18275)
                     /locus_tag="WJK66_000016"
     CDS             complement(16731..18275)
                     /locus_tag="WJK66_000016"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEZ7577798.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQDIKGLFDDFDTTSSRLGSIVADKNKRLAAVLKGV
                     AELDFGSFEDYHIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKF
                     HIELGDTLTNPKLKDSKPFDAVVSNPPYSINWIGSGDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTGIAVNILVLSKHKDNTDIQFIDAGGFFKKETNNNVLTEEHIAEIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEPEDTREIIDIKQLNAEISETVAKIERLRREIDEV
                     IAEIEA"
     gene            complement(18348..19352)
                     /gene="rfaD"
                     /locus_tag="WJK66_000017"
     CDS             complement(18348..19352)
                     /gene="rfaD"
                     /locus_tag="WJK66_000017"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEZ7577799.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKERL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEEFIRYIPFPDALKGKYQSFTQADITKLREAGYTEEFFDVKAGVERYVKWMLENLA
                     "
     gene            complement(19393..20511)
                     /locus_tag="WJK66_000018"
     CDS             complement(19393..20511)
                     /locus_tag="WJK66_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ7577800.1"
                     /translation="MPDFQRITIENIEYFIVDSIQNLRAEDSFIHRNNKLSVFGGNGE
                     ARKYVGSYIDDSGRKLSTFFEYENWGITETKNGRRVEYPLIQKNCFFNKKNLQRYLVD
                     AKVEYHKQEQKYHHDISRFYDDYVLSINNLPTENIFFSIHDVSDHLENSKGYRRGYIR
                     SEDDIWSIWRKIVLPKISYLSILKLLPVKDIEDSEPLFYFRIFLDYQFRSIVHPQLLS
                     REKLEIPASEFNQFVEQEIIKQKSRKGQQKYRKDVINHMPQCPFTLITDEILLRASHI
                     KPYMVCITEKNEKEALDYLNGLALTPTYDWLFDQGYITFLDDGRLICGTRLSRYTWEK
                     LNINPNAKNKMRIYPEGREKYLEYHRKFVFQDNIDDFL"
     gene            complement(20513..21748)
                     /gene="dcm"
                     /locus_tag="WJK66_000019"
     CDS             complement(20513..21748)
                     /gene="dcm"
                     /locus_tag="WJK66_000019"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ7577801.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFAGDIDPYVKKSYLGNYKLNEDFWHNDITQFDARKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGVKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            complement(21785..22756)
                     /gene="hldA"
                     /locus_tag="WJK66_000020"
     CDS             complement(21785..22756)
                     /gene="hldA"
                     /locus_tag="WJK66_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HEZ7577802.1"
                     /translation="MSAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGNDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNCEVLEQIKQKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHAGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNESEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELIKALSGQSTM"
     gene            complement(22811..23551)
                     /gene="pyrF"
                     /locus_tag="WJK66_000021"
     CDS             complement(22811..23551)
                     /gene="pyrF"
                     /locus_tag="WJK66_000021"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEZ7577803.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFSNEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRNLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAASTYL
                     VMGRPVTQAADPVAVLREVNRVANLEAN"
     gene            complement(24077..24724)
                     /gene="adk"
                     /locus_tag="WJK66_000022"
     CDS             complement(24077..24724)
                     /gene="adk"
                     /locus_tag="WJK66_000022"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEZ7577804.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQPVEAVKAEVLGALGK"
     gene            24939..25778
                     /gene="htpX"
                     /locus_tag="WJK66_000023"
     CDS             24939..25778
                     /gene="htpX"
                     /locus_tag="WJK66_000023"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEZ7577805.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSQGGTGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(26079..26639)
                     /locus_tag="WJK66_000024"
     CDS             complement(26079..26639)
                     /locus_tag="WJK66_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7577806.1"
                     /translation="MTANFAQTLVEIQDSLYRVVSTVQYGDDNLKRLTADKRKQYELN
                     FKISEGSTRVESDFKETLVRFGRDMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYL
                     KQVASEGYQTERLYNAVDRLAESQERITSAILKGARGADFVQIGRRSYSREDISEANR
                     RAERVPYGAELVSDGNFTAVLSDIGD"
     gene            complement(26845..27237)
                     /locus_tag="WJK66_000025"
     CDS             complement(26845..27237)
                     /locus_tag="WJK66_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ7577807.1"
                     /translation="MAKSFNQAASELTDIFPNISLTDFDGVNYPVTVNCPMHGNVRYS
                     TFNALIKSKYGCPECAKMSKTQTPPNVGKPLLILDTTTNETLTFPSVTAAGAALGVHF
                     QQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            complement(27354..27764)
                     /locus_tag="WJK66_000026"
     CDS             complement(27354..27764)
                     /locus_tag="WJK66_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ7577808.1"
                     /translation="MALTFTQAVSKLTSKFPHLNLVEFNGVRYPTVIVCPIHGRVTCS
                     TFKSMLDSKSGCPKCASYGVNSHKIPEDTIDKLSKNTVLEDTVTGETLTFPSRASAAR
                     SLGINPAAITDRIKGRVHTETLLAGRYKVHICTK"
     gene            complement(27778..28215)
                     /locus_tag="WJK66_000027"
     CDS             complement(27778..28215)
                     /locus_tag="WJK66_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ7577809.1"
                     /translation="MKISIFWLYNAFILERSIMPKSLSLQDVQLRFSSKFPDKTVLKF
                     TKNYEPVTIQCPLHGSVVYGNLQAAMKSSTGCPECTRNPEKPSPNAVAIRLEDTETGE
                     IYEFASTLAASKFIGCSNSTLAVRLSGRTPFDRLIRYRYRLVK"
     gene            complement(28627..29925)
                     /locus_tag="WJK66_000028"
     CDS             complement(28627..29925)
                     /locus_tag="WJK66_000028"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEZ7577810.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAVLAYYNVQLQHLHNAESVKAEDVMAVIEKVAPRITPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVEACKPIYETMPGWSESTFGVKSYDA
                     LPANAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(30028..31179)
                     /locus_tag="WJK66_000029"
     CDS             complement(30028..31179)
                     /locus_tag="WJK66_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEZ7577811.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQSDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTETVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALGELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPAIERQPAVLVDAE
                     DAEAAHEAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            31514..32893
                     /gene="norM"
                     /locus_tag="WJK66_000030"
     CDS             31514..32893
                     /gene="norM"
                     /locus_tag="WJK66_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HEZ7577812.1"
                     /translation="MLLDLNRFSFSVFLKEVRLLTALALPMLLAQVAQVGIGFIDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGTVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVLWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            33072..34112
                     /gene="murB"
                     /locus_tag="WJK66_000031"
     CDS             33072..34112
                     /gene="murB"
                     /locus_tag="WJK66_000031"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEZ7577813.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADELCDIVRLPEFD
                     RDTVLWLGGGSNILLMEDYAGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHT
                     VALGLNGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPTLGYGDLAAAVAELSAGRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVVSAEKAATLLQRHPDMPHYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDKQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            34124..34774
                     /locus_tag="WJK66_000032"
     CDS             34124..34774
                     /locus_tag="WJK66_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEZ7577814.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLEYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGVRRTLSLLRPYLLPEHREKYDRKIGNGNP"
     gene            34829..35605
                     /locus_tag="WJK66_000033"
     CDS             34829..35605
                     /locus_tag="WJK66_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ7577815.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHGSRVAAIKRSLTSDDI
                     NLPIHLDTFDLGSTDAFQTALWRHHADKPVWFFLLPPSSLTHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDTARECDETATPDPQTESARQILAAEQHLLDSGISNIDILRLGG
                     LYCAERHPVSRLVQKQNIPGGNRPINIVHRNIAVESLFQTAFNPGGRRLKNIIEPRHP
                     TRREFYTEEAAKLGLPAPDFAPDDSVGKIIRTVCDNGLSL"
     gene            35656..36246
                     /locus_tag="WJK66_000034"
     CDS             35656..36246
                     /locus_tag="WJK66_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HEZ7577816.1"
                     /translation="MSALLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGRNIAEQIGGFSRESESANI
                     GNEALADCLDEISRLRDGVVRLNERLDRLERDIWID"
     gene            36266..37156
                     /locus_tag="WJK66_000035"
     CDS             36266..37156
                     /locus_tag="WJK66_000035"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEZ7577817.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            37181..37939
                     /locus_tag="WJK66_000036"
     CDS             37181..37939
                     /locus_tag="WJK66_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ7577818.1"
                     /translation="MNPKTPPDHTEDAVRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NHWEGDTGRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(37994..38659)
                     /locus_tag="WJK66_000037"
     CDS             complement(37994..38659)
                     /locus_tag="WJK66_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ7577819.1"
                     /translation="MAVLSKEQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(38859..39755)
                     /locus_tag="WJK66_000038"
     CDS             complement(38859..39755)
                     /locus_tag="WJK66_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HEZ7577820.1"
                     /translation="MQLLKASDIISHLQIDGIFPGGNAIPCTHLNNYMNIKEKQLMNT
                     IADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGIQSTIARLTMTVYLPAEQKGTHMS
                     RFVALMEQHAEALDFAQLRKLTTEMVALLDSRAGKISVSFPFFRKKTAPVSGIRSLLD
                     YDVCLTGEIKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHNQRSHVTVSLTADAEVGI
                     EEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDMVRDVATSLIADKRIKSF
                     VVESENFESIHNHSAYAYIAYP"
     gene            39824..40981
                     /locus_tag="WJK66_000039"
     CDS             39824..40981
                     /locus_tag="WJK66_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEZ7577821.1"
                     /translation="MKFATKAIHSSYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLRPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHKVKLVWLETPSNPLLRLVDIKVLAA
                     KAKAAGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAVAGPLDCWLVLRGIKTLALRMNAHCQNALEIARRLEAHPAIEKVFH
                     PGLPSHEHYELAKTQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHDVKMEMGIKVGLLRFSIGIEDADDIWNDISAALDTTL"
     gene            complement(41044..42045)
                     /gene="hemB"
                     /locus_tag="WJK66_000040"
     CDS             complement(41044..42045)
                     /gene="hemB"
                     /locus_tag="WJK66_000040"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEZ7577822.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTERAQEAYNPE
                     GLVPSTVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALNEVALDIREGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAIANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(42228..42512)
                     /gene="yhbY"
                     /locus_tag="WJK66_000041"
     CDS             complement(42228..42512)
                     /gene="yhbY"
                     /locus_tag="WJK66_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEZ7577823.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            42622..43242
                     /locus_tag="WJK66_000042"
     CDS             42622..43242
                     /locus_tag="WJK66_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEZ7577824.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINEKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGTSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            43306..45273
                     /gene="ftsH"
                     /locus_tag="WJK66_000043"
     CDS             43306..45273
                     /gene="ftsH"
                     /locus_tag="WJK66_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEZ7577825.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDKSVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASTESEQQPENKA"
     gene            45495..45926
                     /locus_tag="WJK66_000044"
     CDS             45495..45926
                     /locus_tag="WJK66_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEZ7577826.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPNFLPWYSKTEVIGRNG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            45919..46197
                     /locus_tag="WJK66_000045"
     CDS             45919..46197
                     /locus_tag="WJK66_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEZ7577827.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            46250..46828
                     /gene="pth"
                     /locus_tag="WJK66_000046"
     CDS             46250..46828
                     /gene="pth"
                     /locus_tag="WJK66_000046"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEZ7577828.1"
                     /translation="MSNTIKMVVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARATLPDGDVWLLKPTTFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQARLGTADYYRLRLGIDHPGDRNLVVGYVLNKPSAEHR
                     QQIDDAIAKSLQAIPDIISGKWEEATRFLHSK"
     gene            46888..47094
                     /locus_tag="WJK66_000047"
     CDS             46888..47094
                     /locus_tag="WJK66_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEZ7577829.1"
                     /translation="MSELNRLIQTESVAVIEETLNFLLYECSIDDAPSAEEIAVWRDM
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            47091..47453
                     /locus_tag="WJK66_000048"
                     /pseudo
     CDS             47091..47453
                     /locus_tag="WJK66_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
     gene            47684..49099
                     /locus_tag="WJK66_000049"
     CDS             47684..49099
                     /locus_tag="WJK66_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13/DASS family transporter"
                     /protein_id="HEZ7577830.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMIPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGSMKAAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDREKERKTYVFVLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSSAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            49218..49727
                     /locus_tag="WJK66_000050"
     CDS             49218..49727
                     /locus_tag="WJK66_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ7577831.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIEGVSTKRHGYHDDVPSEP
                     VVIIKAEAV"
     gene            49890..51272
                     /locus_tag="WJK66_000051"
     CDS             49890..51272
                     /locus_tag="WJK66_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEZ7577832.1"
                     /translation="MANIARDIFKAYDIRGIVGKTLTDDAAYLIGRAIAAKAAEKGMT
                     RIALGRDGRLSGPELMEHIQRGFTDSGIGVLNVGMVATPMLYFAAINECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIVEKDGFVAADKQGNVTEKDISGEYHNHI
                     VGHIKLKRPMKIAIDAGNGVGGAFAGKLYKGLGNEVTELFCDVDGTFPNHHPDPSKPK
                     NLQDLIAALKNGDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLNRNPGAK
                     VIFDVKSTRLLAPWIKEHGGEAIMEKTGHSFIKSAMKKTGALVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQSISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQAAIERIQNQFKAVI
                     ESNPNLIWPL"
     gene            complement(51292..52047)
                     /locus_tag="WJK66_000052"
     CDS             complement(51292..52047)
                     /locus_tag="WJK66_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ7577833.1"
                     /translation="MIKIRNIHKTFGKNTILRGINLDVCKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSTKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(52057..52773)
                     /locus_tag="WJK66_000053"
     CDS             complement(52057..52773)
                     /locus_tag="WJK66_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ7577834.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAVALVRIMPAGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(52763..53590)
                     /locus_tag="WJK66_000054"
     CDS             complement(52763..53590)
                     /locus_tag="WJK66_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7577835.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSESGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            53875..54783
                     /locus_tag="WJK66_000055"
     CDS             53875..54783
                     /locus_tag="WJK66_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEZ7577836.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALSA
                     LKTRFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGHDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
BASE COUNT        13018 a        15078 c        13510 g        13219 t
ORIGIN      
        1 taatccacta tagattaaaa tcacacaata tccaactatt cggaccttca tatgacacat
       61 accgtccacc tgcatttaga agaaaccgac aacttggcgt tgcagcgtct gtgcggttct
      121 tttgacaaca accttgattt acttgctaaa gcactcgata tccacatcag ccgccgtttt
      181 gaacatttca ctttcaacgg cgcatttgca cacgccggca aacgcgcact gctcaaactc
      241 ttggaaacgg cgcagacgcg cgacctaaac gacggcgaca tcaggcttgc cgccgtcgaa
      301 gcccaaaccg aagatgccgg tcatcaagaa aaaaaccatg accacgccta ttatttccgt
      361 accaaacgcg gcagcatcgg cggcagaacg ccacggcaga acggctatat ccgcgcccta
      421 ctcaaccacg acatcgtatt cggtctcggg ccggcaggta cgggcaaaac ctatctcgcc
      481 gtcgccgccg ccgtcgatgc gatggaaaaa caccaagtcg aacgcatcat tttagtgcgg
      541 cctgccgtcg aagccggcga gaaactgggt tttctgcccg gtgacctgac ccagaaagtc
      601 gatccctacc ttcgtccgct ttatgatgcc ctctatgacc tgatgggctt tgaccgtgtc
      661 accaagctga ttgaaaaagg cctgattgaa atcgccccgc tcgcctatat gcgcggcagg
      721 acgctcaacg gcgcatacat catcctcgac gaagcgcaaa acaccacgcc cgaacaaatg
      781 aaaatgttcc tgacccgcat cggcttcggc gcgaaagccg tcattaccgg cgacaccagc
      841 caaatcgacc tgcccaaaaa catcaaatcg ggattaaaag atgcgcgtga gaaactgcac
      901 ggcgtggaag ggctgtattt ccacaccttt accggcgaag acgttgtccg gcatcctttg
      961 gtgcaaaaaa tcgtcgaagc ctacgaatcg gcagaacacg actgacattc caatgccgtc
     1021 tgaaacccga tgcatggaaa atcgggcaga gaaaaacaga tacggaagcc aagctccgta
     1081 tccgtatccg tttctttatc cgtccaaccg atacatcaat cgaattttct gcatcggatt
     1141 gcctcatctt cgcaaaacat tgccgaagtt tcgcggttgc acgcctgacg cactttctgc
     1201 cgcgccacgc cttcattccg atcgcttgcc tcaaacgtct tctgaaaagg cgtaacgctg
     1261 catacataca tggtggaatt tgaaccgaaa aggtcgattt tgacggtatt gctgccgctt
     1321 tccaatacgg cgacacggtg ttccaactgc gccagacgtg cttccaaatc gccgattctg
     1381 tcggcaaatg caggcatggc ggcacacgcg gtcaaaatgc ccaaaataca ttttttcatc
     1441 aatttttcct tgtttcttga accctgccca cccggacatc ctttttccga gaggcagggt
     1501 tagactttat ttacggggat gaatcccctt acaaatcagg ataatacata gaacggcact
     1561 ttatgcttca tcccatgtgt tgaaacttca ttgctccttg tctgttgacc cttattccca
     1621 atccgttccc acaaccttcg gcttgggttt gcgcctgcga cggcggcgtt ttttcgcctg
     1681 tccttcgttt ttttcgtgtc gggcggcggc ttcgtttttg gtcatctccg accgctgttc
     1741 cgtcgatgcc gtctgaaacg ccgtccacca ctcggcaagg gcgcggtccg cattgccggt
     1801 ttcggcgcgc aagagcagga aatcataggc ggcacggaaa cgcgcctgtg caaacagttt
     1861 gtgcggacgt gcgcctttgc gattttcaaa ctgcggctgg aacatccaaa tttcgcgcat
     1921 cgtggcggaa aagcgttgcg gcacgcccca accgcgttcg acggtttcgc gcatcgtatt
     1981 gattgcatcg gacagggcgg gcgcgggttt caaaccctgt tgcagattgc ttttccaatg
     2041 gcgttccaac tcgggccaca tcagagccgc caatacaaaa ccgaccgaaa ccgatttgtc
     2101 ggcacgcagc cgctcatcgg tatttttcag ggcaagcacc gtcatttttc cggcgatgcc
     2161 gtctgaaaca cgcaaggcat tgagcagcgg atggatgtcg tccggtatgt caaatccgtt
     2221 caaacgtttc agacactcgc gcgcgtgccc tgaaaacagc agtttcatga tttcgtcaaa
     2281 cagcctcgct accggttcgt gcttcagacg gcatatcgat tcggcaatcg gtgcggtggt
     2341 ttcttccgac agctcaaagc ccaatttgcc cgacaggcgg atggcgcgca aaatcctgac
     2401 agggtcttcc tgatagcgtt cggcggcatc accaatcata accagcctgc gggcggcaac
     2461 atcggcaatc ccgttgtgga aatccaaaat ctcttctttt tcaggatcgt aatacaaggc
     2521 attgcaggta aaatcgcgcc gcatcgcatc ttcttcgatg ctgccgtagg tgttgtcttt
     2581 catgattctg ccgtgcgcgt tctgatgaac cttcgcaccg ccgcgaaacg tcgttacttc
     2641 gataatctcc gcaccgttca tcacatggac gatttgaaaa cgcctgccga tgatgcggct
     2701 gcggcggaag agtttgtgca cctgttcggg cgtggcatcg gttgccacat cgaaatcctt
     2761 gggttcgatg ccgagcagca gatccctgat cgcgccgccg accacataag cctgaaaccc
     2821 cgcccctttc agacggcgta tgacgttttc ggcggcaaag ctcaacattt cggcacggat
     2881 gttgtgtctt tcggcgggaa tgaccgtttt actttccgct tttttactgc tccgaccgga
     2941 aggcagcatc ttattcaacc attttttcag cataaatatt ctgacccgaa gagcggcatc
     3001 tccacaggca accgtgttca gacggcatca ggcgcggttt gcaagatgcc gtaaccataa
     3061 acaaaaggcg tagaaactac gccgtccgaa tcacgccgcc ctcgcggcgg caacgcgtca
     3121 ttataacaga ttgccctcca aaaaaatcag accctgcttt tgtggcggag tctgaaattt
     3181 gatgcacctt ctccgattgg gaacattccc gtcgtacaaa caggcaaatg aaggaacaga
     3241 aatcacattc aaacagtgtt ccgccgcctc tacgcggctt cccagtaatc gatatacagc
     3301 tgcaattcga gattgttgcg ccattcgttg gcaacggggc ggtaaaccgt gcggatgtat
     3361 tcgggaatgt cttcgctgca acgccaaaac atcgcttcaa attcgcagcc gtctttttgc
     3421 agccagactt ttttgtgttt gccctccgcg cccaaaggtt gctggcggac gacgtggaac
     3481 tcgtcggtaa agctcggcgg cgcgaagccc tgcccccaaa cgtgacgggc aaggttttgc
     3541 gcctgttcca acgtgatgtc gcaggcgggc aggctgccgt cggtgatgaa ggtttgcgac
     3601 aaatcgtctt cgcacaccat ttcgcgcacg gcttcttcaa aggccgtctg aaacgcggga
     3661 atgttgtgtt cgagtatgct caagcccgcc gccatcgcgt gtccgccgaa tttcaaaatc
     3721 aaatcgggat ggcgtttgga caccaagtcc aaagcatcgc gcaagtgaag attgggaatg
     3781 gaacgccccg aaccgcgcac ttcaccgttg tcggcaggcg caaacacgat ggtcggacga
     3841 taaaaacggt ctttgaggcg gctggcaacg atgccgacca cgccttgatg gaagtcgtcg
     3901 cgatacgcca ccaaagtcat ctgacctgaa ggcagggttt caggaaaagc attcagcgcg
     3961 tcttgcagca tagactgctc gatttcgcga cgctcgatat tgaggttgtt taactgagcc
     4021 gccagttcct gagcttcgga atcatctcgc gccaacaggc aggcgatgcc gaccgacata
     4081 tcgtccagcc gtccggcggc gttgatgcgc gggcccaacg caaaacccat atcaaacggc
     4141 tgcgccttgc gccaatcgcg ccgcgccact tcaaacaagg cgcggatacc ggggcgcatt
     4201 ttgccggagc gcatccgttt caaaccttgc gacacgagga tgcggttgtt gtggtcgaga
     4261 gggacgacat cggcaaccgt gccgagtgcg accaaatcca aaaggtcgcc cagattcggc
     4321 tctttgatgc cgtctgaaaa ataatcgcgg cggcgcaatt cggcacgcaa cgccgtcaac
     4381 acataaaaaa tcacgcccac gcccgccaag cttttgcttg gaaaaccaca gcctttttgg
     4441 ttcggattga cgatgatgca gtcgggcacg gtatcggcag gcaaatggtg gtcggttaca
     4501 atgacatcca aacccaattc ctgcgccctc gccacgcctg cgatgctggc gataccgtta
     4561 tcaaccgtaa tcagcaaatc cacgccttgc gcggcggcga tttcggccag ctcgggcgtt
     4621 aagccgtagc cgtgttcaaa gcggttgggc acgaggaaat ccactttcgc gcccatcgcc
     4681 gccaaaccgt ccaaaccgac ggcacacgcc gtcgcgccgt cggcatcata gtcggcgaca
     4741 atcaggattt tttcctgacg ctcaaccgca tccgccaaac ggcgggcggc ggcttcgcaa
     4801 ttcgtcagcg attgataagg caggagggaa gcgagtttgt cgtccaattc ggcaggactt
     4861 tgcacaccgc gcgaggcaca aagccgggcg attaaaggat cggcgccggc ggtgagcaaa
     4921 tgattgaaaa cgtcggtatt gacggatcgg gtttggattt tgacggacat agcgtggaac
     4981 tttacagtgc ggatgtgttt gaaatgccgt ctgaaggctt ttcagacggc attgccgttc
     5041 atttgtctga ggaattcgtg gctgcaatca acgccgcgac aggcaaggcc agcaccgcgc
     5101 ccaccgcatc cagtatcaac gtggtgggcg tatatgcaat attttcaaac aacttggatt
     5161 tgtcggtatg ttttttcgta accgtgtaat aaatatcggc aggcacactt tgctcaaaat
     5221 gataatcggc gttcagtttt tgcggtgtgg cgtagtattt gcctttggcg gagacgcagc
     5281 gcgtgtaaat ggtccgattg tcgagttcga ccgcttcaaa ctcaagctgt ttcagcttgg
     5341 cgatgtcggc aggtctgtcg gtatcgtagc gcaggcaaag gccttcggta ctgaaattct
     5401 ggctggcggg cgattcgagt ttgaccggca gggcttggta ggcaaagcgc gggttgggct
     5461 caaccatttg aaactgcttg tccaacccgg ccttcaaaat gcccgtcagc ttcgccgaat
     5521 cttcaggatt gacgacgaac cagtatttcc cgcccatcat caccaggctg cccttttcca
     5581 attgggcatt gtcttcggca accacaccga aggcgcggat ttggtccttg tcaacgtgtt
     5641 tgcggtcggt cgtttggctg aacgggtcgt tcatacccca caacataaac gtacagccat
     5701 tcagcatcaa ggttgccgcc aaaacggcgg cggtcgtttt acggaacaac attttatcct
     5761 ccaatctaaa taacggcgga tgtcgtctga aaatatgcct aaatttccgc gtccccgttt
     5821 tccataacgg ttttcaattc ttgaaacagg gcgcggaaat ttttcggcgg tttgttttgc
     5881 tcctgctctt ttttggtgtt gcggatcaag gtacgcaatt taccggcgtc cgcatgcgga
     5941 aaatccgaca taaactgcgt caacgcgccg tcgtctgcca acagccgtac gcgcgcctgt
     6001 tccacgcgtt gcaaaaaggc gttgtgcgcc gcatcgtcgc cgcgcagctt ggcaagaaac
     6061 gcctcgatgg gcgcgggatc cgtgtcgcgc atcagcctgc cgataaattg cgcctgccgc
     6121 ttgagcgcgc cgttggatgt gattttttta taggcggtta ccgcctcgta caaatccgca
     6181 tccaaaccga tttttttcag cgtatcgttt gagagcttgg tcaacgccat acccaaatcc
     6241 tgcaaatcgt tcatctgctt tttcatttgg gttttgctga tccattcgtc ttcttgttca
     6301 aacatcttga ttctcgaatt tttccaatag cgcattttaa cagaatgccg ccgcgttcag
     6361 acggcatttt gcaccgtccg ccctgcaaac ggcgcgcttt tgcccctgcc gcccgaaacg
     6421 gttaaaatgc cgcaaagcat catcaagaaa gatttctatg ctgttcaacc acaccgcttc
     6481 cgaactcctc gacctctgcc gccgcacgct cgacttggca aaagcagcgg gcgcgaccgc
     6541 cgccgaagcc gatttcagcg aatccatcgg acaaagcgtc agcgtgcggc tgggcgaaat
     6601 cgaacaaatc gagttccagc aggacaagtc gctggacatt accgtttacg tcggcaaacg
     6661 caaaggccgc gctggcaccg ccgatttctc cgaacaagcc ctgcgcgaca ccgtccgcgc
     6721 cgccgccgac atcgcccgcc acaccgccga agacggttgc gccggacttg ccgaccccga
     6781 gttgatggcg caacacatcg gcgaccccga cctttaccac gaatgggatt tggatacgga
     6841 agccgccgtc ggcttggcaa aacaatgcga acaagccgcc ctgaacgagg atgagcgcat
     6901 cgaaaactcc gaaggcgcgg cggtgcaaac cggccattac caatacgttt acggcaacac
     6961 ccacggtttt gccgcacacc ggcaaggcac gcaccacagc atttcctgca gcgtcgttgc
     7021 cgccgacgaa aacgggatgc agcgcgacta ctggtacgat tccgcctgcc gccatccgga
     7081 tatggacagc cccgaaacca tcggtcaaac cgccgcccgc cgcactttgc gccgactcgg
     7141 cagccgcagc atcccgacag gcagctaccc cgtcctcttc gataccacca tttcgggcgg
     7201 tcttatcgga cacctcgtcg gcgcactctc cggcggcgcg ctctaccgcc aaagcagttt
     7261 cctgatcgac agcatcggca aaaaagtcct gcccgatttc ctcaacctgc gcgaagaacc
     7321 gcacatcccc cgctctttcc gcagcagcta ttttgatgcg gaaggcgttg ccaccgcacc
     7381 gcgcttcgtg attcaaaacg gcattgtcga aggctatttc ctcagcagtt acagcgcgcg
     7441 caaactcggt atgcagacca cgggcaacgc cggcggcgcg cacaacctgt atttgaacca
     7501 tacgcacgaa acgcaatccg acctgctgaa agaaatgggg acgggattat tggttaccga
     7561 actgatggga cagggcgcga acaccattac cggtgactac tcgcgcggcg cggcgggttt
     7621 ttgggtggaa aacggcgtga ttgcctaccc cgtccacgag attaccgtag ccggacgctt
     7681 gcaggatatg taccgcgaca ttgtcggcgt ggcggatgac gctttgcggc gttcgtccaa
     7741 caaaatcggc tcgattctga ttgcggggat gacggttgcc ggaagctgaa cgccctgccg
     7801 atgccgtctg aagacgaagg cagcacgttt tgccccgtgc ctacccgttt gccgttttat
     7861 gccgcaccgc cttttgccga caacattgca cattttttca tcagcactga ttttccctta
     7921 tccgtcaaaa tactaagcca cttgtccaca acagggtaca cacaagttca ggataaggcg
     7981 tgcggggatt ggggtttttc cgccgtcggc aaaacggcag acaaaaacgg cgggacattg
     8041 cctgtcccgc cgttttcaaa tatacatttg accgtacatc aggaattaag cagcctgaat
     8101 gttggcggcc tgtttgcctt tagggccggt ggttacgtcg aaagagacgc gttggccttc
     8161 tttcagggtt ttgaaacctt ccatattgat tgctgagaag tgagcgaaca aatcttcgcc
     8221 gccttcatca ggagtgatga aaccaaaacc tttagcgtcg ttaaaccatt ttacgatacc
     8281 ggttgccatt agaaacttcc tatactcaaa aattaacaaa atcagcaaaa caaggcatac
     8341 ggcaaaccga gccgtctggc gttccttcca gcttgatata ctcggcggat gcccaatcct
     8401 gcttaaccgt ctttttacat ttgttaagac aaaacgtcaa gctatgtttt caaaaaagtg
     8461 ggaaaatagg caaaagtacc gaaaatacaa caaaaatgcc gtctgaacgc ggctttcaaa
     8521 tgggacacac tatgaccgcg caacaccaat ccgacacgct cttgcaccgt ctgaacaccc
     8581 tgccgccgaa acgttacggc gttttcctat tgaacgacga ttacaccacg atggaatttg
     8641 tcgtcgaaat cctgaccgaa atctttatgc tcggacaaga acaggcggta gcggtgatgc
     8701 tcttggttca tcacgaaggc aaaggcctgt gcggcactta cacgcgcgat attgcccaaa
     8761 ccaaacaaca acaagtcatg cagcgggcaa aagccgaagg gcatccgctg caatgtattg
     8821 tcgaggagat ttaatatgct tgcacccgaa ttggaacaga ttttgcagca gctttaccgc
     8881 gaggcgcgta aggctcatta tgaatttatc agcctcgagc atctgctttt ggtactcatc
     8941 gaagaagatg cctccgtgcc gaacgtctta aaactatgcg gcgcggattt gaaagtggtg
     9001 tccgaacagc tcgccgccag cgttgccgaa aacacccccc tgattcccga acacctttta
     9061 gacacggtcg aaacccggcc cacgctcggc ttccaacgcg tgatgcaacg ggcgatggtg
     9121 catacccagt ctgccggaaa agccgcagtc gaaccgttgg acgttttggt cgcgctgatg
     9181 agcgaaaccg acagccacac cgtctatttc ctcaagctgc aatcggttac gcgttttgaa
     9241 gttttgcgct gtattgccca tggctcgccc gatgacgagg atggcaatga ttcagacggc
     9301 ctgggacgcg agggcgaaga agcggaacaa aaaaccggct ccctttccga ctacaccgtc
     9361 aacctcaaca ccgaagtcaa agccggccgt atcgaccctt tgattggtcg caaacacgaa
     9421 atggaacggc tggtgcaaat cctatgccgc cgccgcaaaa acaatccgct tttggtcggc
     9481 gaagcaggcg tgggcaaaac cgcgctggcg gaaggtttgg cacatcaaat cgtcaacggc
     9541 ggcattccag acgcgcttaa agatgccgaa gtgtacgcgc tggatatggg ctcgctgttg
     9601 gcgggcacga aataccgtgg cgactttgaa gcgcgggtca aatccgtctt gaaacagctc
     9661 gaaaaaatcc cgcacgccat tttgtttatc gacgaaatcc acaccatcat cggcgcaggc
     9721 agcaccagcg gcggcaccat ggacgcgtcc aacctgctca aacccgcgct ggcaaaaggt
     9781 tcgctgcgct gcatcggcgc aaccacctac gacgaatacc gcaccatttt cgacaaagac
     9841 tatgccttaa gccgccgctt ccaaaaaatc gacgtggtcg aacccaccgt ttccgaaacc
     9901 gtacaaatcc tgcgcggctt gaagccgatg tttgaagcct tccaccaagt ccgctatacg
     9961 caaggcgcac tcgaagccgc cgccgaactc tccgcacgct acatcaacga gcgtttcctg
    10021 cccgacaaag ccatcgacgt gatggacgaa gcaggcgcgg cgcaacggat tctgcccaaa
    10081 tccaaacaga aaaaagtcat cggcaaagcg caaatcgaaa ccgtcatcgc caaagtcgcg
    10141 cggattccag aaaaaaccgt gtcgcacgac gacaaacagg tgctgcaatt cctcggccgc
    10201 gatttgaaaa acatggttta cggtcaagaa aacgccatcg acgcgctggt tgccgccgtc
    10261 aaaatgtcgc gttcgggctt gggactgccc gacaaaccga taggcagctt cctcttctcc
    10321 ggcccgaccg gcgtgggcaa aaccgaagcc gccaaacagc ttgcctactc gatgggcgta
    10381 ccgctgcaac gctttgatat gtccgaatac atggaacgcc atgccgtatc gcgcctcatc
    10441 ggcgcgcctc cgggctacgt cggcttcgaa caaggcggcc ttttgaccga agccgtcaac
    10501 aaacagccgc attgcgtgtt gctcttggac gaaatcgaaa aagcccaccc cgacattttc
    10561 aacgtcctcc tgcaagtcat ggacgcaggc aaactgaccg acaacaacgg caagagtgcc
    10621 gatttccgca acgtcatcct gattatgacc accaacgcgg gcgcggaaag cctcagccgc
    10681 cccggcctcg gctttaccgc caagcgcgaa cgcggcgacg aaatgcaggc catcaacaaa
    10741 ctcttcacgc ccgagttccg caaccgcttg gacgcgatta tcccgtttgc acccctgtcc
    10801 gaacccatta tcgccaaagt cgtggacaaa ttcctgctcc ggctcgaaca ccggctcctc
    10861 gacaagaaag tcgaagccga attcacaccg gcattgcgca aatatctggc gagaaaaggt
    10921 ttcgacccgc aaatgggcgc gcgcccgatg caccgcctga ttcaggaaaa aatccgcaaa
    10981 ccgctcgccg acgaactcct gttcggcaaa ctatccaacg gcggcttcgt gcggatagac
    11041 tgggatgcgg caaaagaaga agccgtgttg aaatttaaga aaagcaaggt taaacctgaa
    11101 acagaaactg tttaaccgat tgaaaaataa aatgccgtct gaaatttcag acggcatttt
    11161 tttaaatatc tgtccctatt ccggcaaact tttccacaaa cgccgcaatc ttttgaaaaa
    11221 cactttgttt cttcgtcaga tattgcgggt tgagcgggct cattttcggc agggtttcgg
    11281 tcagttccgt gccgttttcg ctggcatagc cgcgtttgag cgagccggtc aaataacgtt
    11341 tggcggcggt ttcgttcagg tcttcggcgg caatcagctc tgctgcttcg cgcctcatca
    11401 cctcttgagc gtaggtgtaa aaggtttcca gtatggcagg aacgtcgggg actttgtcca
    11461 aatccgtatc gttgatgaaa tccacaatca ggctctcttt ggcgcggtgg ccgatgctgg
    11521 cgcggatgat gcggcggatt tcttccacca gctccgcttt gcctttgatt tttttgtggt
    11581 gctcgaaaac cagttgcaag atgtaatcca gattgatttc ctgcgatttg agcaaatcca
    11641 cctcaaaaac cacatcgtcc caatcgattt ttgattgttc tttctgttcg cctgcttttt
    11701 ggcggcgcag ccagtcgcgg atgtcgttat aggcagaacg gtagtcctgc actgcccttt
    11761 cagacggcat cggcacttgc cgcatttcct gcacgtcttc atcgctcagg tagtattttt
    11821 cttgaaacgc cttcatcgca tcttcgtccg ccgcgtccac actctgcaac tcgcgcagcg
    11881 cggcaaattc atcgtagttc tgcaatacgt tttccgcccg caggtattcg ccgaagagtt
    11941 tggcaaaatc ttttttgtct ttttccgttt cgattttgtc gggatcgggg aaacgctcgc
    12001 gcaattcttt tgccacatcc aaataaccgc gccgcacttc gcccgtccga tcatcgatat
    12061 agccgttcat gtattcttcg tagctttttt ccaacaccac gtttttggtg tttttatcgc
    12121 cgaacagggt aatcgcatca atggttgcct gctccaaatc gcggaagcag acaatattgc
    12181 cgaaggtttt ggtggcatcg taaatgcggt tggtgcgcga aaacgcctgc atcaggccgt
    12241 gatagcgcag gtttttatcg acaaacagcg tgttcagcgt cggcgcgtca aaacccgtca
    12301 aaaacatacc gaccacaatc agcaaatcca cttcctggtt tttcacccgt tttgccaaat
    12361 cgcggtagta gttctgaaag gctttgctgt ccgtgccgaa attggttttg aaacaggcgt
    12421 tgtaatcgtt gatggcagcc tgcaaaaatt ctttcgcgct gctgtccatc gcttccggtt
    12481 caaaggtctc atcaacaatt tcaccgacgg cgttctgctc ttcgttggcc gcaaaggaaa
    12541 aaatggtggc cactttcagc gggtgcaggc tgcctgcctg ttgtgttttg aacgcttcgt
    12601 aatagcactt cgccgcatcc acgctgctga ccgcaaacat ggcgttgaag cctttaccgc
    12661 ccgcattcag gcggtgcgtt ttctgcctga actggttcag gatatattgc gtgatttcgc
    12721 ggatgcgttc ggggtgcagc agggcttggc ggttttcggc agcactcagt ttcttctcat
    12781 cctgttccgc ttccacggct ttgaactgcg ggcgcacatc gttgtaatcc actttgaatt
    12841 tcaatacttt ttcatcgcgg atggcatcgg taatcacata agaatgcaac tcacgcccga
    12901 acacgcccgc cgtggtttcc gcgcccaaag cgttttcggg aaaaatcggc gtaccggtaa
    12961 aaccgaactg gcagaatttc ttaaatttct ttttcaggtt ttttgcgctt cgccgaattg
    13021 cgagcggtgg cattcgtcga aaataaatac aacctgctga tggtaaaccg gcagattatc
    13081 ttcactcttc atcaggttgt tcagcttttg gatggtggta acgatgattt tgttgtcgtc
    13141 tttttccaaa ttgcgtttca agcccgccgt gctttccgaa ccattcacgc tgtcgggcga
    13201 aaaacgttgg tattccttca tcgtctgata gtccaaatcc ttcctgtcca ccacgaagaa
    13261 aaccttgtcg ataaacgccg attccgtcgc cagacgcgcc gccttaaagc tggtcagcgt
    13321 tttgccgctg cccgtggtat gccaaacata gccgccgctt tccggtttgc tccaattctt
    13381 tacctacgcc gagctgttga ttttccacaa aatgcgttcg gcggcggcaa tctgatacgg
    13441 ccgcataatc agcagcgtat cattcgcatc gaacacgctg taatgcagca atacgcccag
    13501 caatacgctt ttctgcagga acgtggcggt aaagtctttc aaatccttaa tcggatgatt
    13561 gtccgaccgc gcccaattca tcgtgaaatc aaagctgttt ttgtcgcgct tggtggtgtt
    13621 ggcgaaatag cgcgtgtccg tgccgttgga aatcacgaag atttgcagga atttgaacag
    13681 cgaattttcg ctgttgaagc tctctttgct gtaacggtgc acctgattga atgcttcgcg
    13741 caccgccacg ccgcgctttt tcaattcaat ctgcaccagc ggcaggccgt ttaccagcac
    13801 ggtaacgtca tagcggtttg catacgtgcc cgtctgctca aactgattga taatctgcac
    13861 atggttgcgg gcaaggtttt tcttgtccag cagataaatg tttttcagac ggccgtcatc
    13921 aaaagcgaaa tcataaatat ggtcgtcatg gattttgcgg gttttatcgg taatgttttc
    13981 agacggcctg tccagatatt ccgtcaaaaa ccgcgcccat tcgccgtctg aaaacgccac
    14041 atcgttcagc cgctgcaact gcgcgcgcag gttttccagc agcctgcttt ggctgttcaa
    14101 atccttgcgg tattcgtaac cctgattctg caaatcggcg attaactccg cttccaactg
    14161 gttttccgat tggtagctgc ccgactgttc gattttttca tattggtcga gcacgatgaa
    14221 attcggcgtt tcggcgatgg gtttggtttc gaggttcatg ggtcgtcctt tgcagtatcg
    14281 ggttttaaac ggtttgatgt tcggattggg ctgcctgaaa atgtgcaggc tgctttttca
    14341 ggtagcctta gggaaggcaa gcagctgttc gcggtaatat tcgtattgtt tgcggcgcag
    14401 ggcgatttcg tgcggcaggc cttcgctgat agagtgggtc agggtatcga atttgtctag
    14461 gatggcggca attttttcct gttcggggag tggtgggatg gggattttta atttctttaa
    14521 ttctttcatg ctaataatag gtaaacttga agtattaata agttttttgc tctgctccgc
    14581 aataatgaaa aaataataga aagcgaaatt tatatctaaa gcatccttaa attcatcttt
    14641 aattgtaaaa ttggtcagtt gttggttact aatataatta acttttatta acgcatgctc
    14701 tcctatggtt gccgttgtcg acatcattat tgatttagca ggaaacagct tgccgccctt
    14761 aactgcgctt tttgatatat gcttcaatga attatctaaa atacggctat tttccctaat
    14821 atcttccatt ctaaaccaag ggattgagcc attttcccaa tattctttat ttgatttgga
    14881 aggggtatag ccattcttca agtcaaacac ctcccccaac gtcttccaaa ccacatcttt
    14941 cagacggcct ttatagccat cagctatccc ccccccgatt tgattgtcaa aatctaaaag
    15001 aaagtcgcgg taataccggt attggcgttt gcgcagggct aattcagctt ccagctcggt
    15061 gaatttgtca agtattttta caattttttg ttgggtttcc aggggtggga tagggattaa
    15121 taattttttc aagttactgg catttagagc aggaatacct gtaaatgtac ataaggcatg
    15181 aatctcttgt tcaaattcaa gtaatatatg atagagaaaa cgaacatcca attctgttct
    15241 attcttaata gtaactgcat aatttaagtt gccacgaaaa tatcttcctt cttgccacgt
    15301 tattgagccg actcccgctc ctcttgttgt gatgccgata gggtcatttt cagtgttcca
    15361 ttcatcaata tagccaagcg gctctttgcc actgttaata actggataac taccaatatt
    15421 atttgagatt ttctgcttac ttacagcttg tcctgttttt atgatagcta tcccattttc
    15481 tccacccaac ggcttccact ccaccggtgc cgtctgaatc atctcaatca attttttcgc
    15541 tttgctttgc atatccatgg ttgttttccg tttctttccc gctgtggcgg gatgtggggc
    15601 ggttgtgggg tgcttcggct cactctgtcc gtttgccgtt atttctgcgg gctgaagtcc
    15661 gtcctgcttt caggctgcct gcaatggtgt gcaggctgct ttttcaggta gcctgaatgg
    15721 tagttatttt gtgcggtctt ttttctgttt gatctgttgc acggctgttt tttccaacgc
    15781 tttaacgctt tccagatagg cttgctccac cggcgacagg atgcgcgctt cataggcgcg
    15841 gtattcgcgt tcggctttct gctctgcctg tttgcggctg atgctgcctg caccctgtaa
    15901 tgtgccttgt ccgtacagcc cggaaaattt gtccaattcg gctatccagt cgcgcatata
    15961 catggggctt tgctcctgcg ctttgatttc cgccaggtcg aagaaggcgg aaaccagacg
    16021 gttcaggcgg aacagttcgt cttcggtcag atagtttttg gcgattttgg cttcattcag
    16081 cgtggggatt gcgccttgaa aggtggtcag ccccataaag tctttgctgc tgtcggcacg
    16141 gctgtatatc agctcagctg cggtttgccg gctggcggca tagtgcagtt tgttttgaac
    16201 ggcggcaaaa aaggtttggc tttcgctgct tttggggttg tagtcttggc tggtggcata
    16261 taaatcaagc acttgccggt atagggcttt ttcgctgctg cggatgtcgc gaatgcggtt
    16321 gagcagttct ttccaatagt cgccgccgcc tgtgcctttc aggcgttcgt cgtctatggc
    16381 aaagcctttg gtcagatatt cgtccagccg ttcggttgcc cattggcgga attggatgcc
    16441 ccgcgcggaa cggacgcggt agccgacggc aataatcatg ggcagggaat aatgggcgat
    16501 tttgcggttt accttgcgcc cgttttcatt ttgaactgtc aactggaagt tgacagttgc
    16561 cttctcttcc aattcttgct ctgcaagaat ggttttaatg tgtttgctga tattttgttt
    16621 ggtggtttgg tacagttctg ccatgtccgc ctgcgtcagc caaagctgtc cgccaaattc
    16681 ctgcaaggca aattgggcag tgccgtcgtt ggcggtgtat aggatgatgc tcatgcttca
    16741 atctctgcaa tcacttcgtc aatttcacgc cgcagccgtt cgattttggc aacggtttcg
    16801 ctgatttcgg cgttaagctg tttgatgtcg ataatttcgc gggtgtcttc gggttcaaca
    16861 tagctgctga cggcgaggtt gtagccgttg tctttgacgg tttgctgggc ggcgttttgg
    16921 gcgatatgcg gcacatcggc tttatcggcg aagagtttga cgatttcggc aatgtgttct
    16981 tcggttaaga cgttgttgtt ggtttctttt ttaaagaagc cgcctgcgtc gatgaattgg
    17041 atgtcggtat tgtctttgtg tttggacaaa accagtatat tgacggcgat gccggtgccg
    17101 taaaagagat tgggcgcaag ggcgatgacg gtttccacgt agttgccctc caccagatat
    17161 tggcggattt tctgctctgc gccgccgcga tagaaaatgc cggggaatga gacgatggcg
    17221 gcgcggcctc tgccggaaag gtagttcagt gcgtgcagaa tgaaggcaaa atcggctttg
    17281 gatttcgggg cgagtacgcc tgcaggggca aagcggtcgt cgttgattaa ggtggggtcg
    17341 ccgctgccta tccagttgat ggaatagggc ggattggaaa cgacggcatc aaagggtttg
    17401 ctgtctttga gtttgggatt ggtcagtgtg tcgcccaatt cgatgtggaa tttgttgtaa
    17461 ttgacgttgt gcagaaacat attcatgcgg gcgaggttgt aggtggtgtg gttgatttcc
    17521 tgcccgaaga agccttcttc gatgatgtgc tcgtcaaact gttttttcgc ctgcaagagc
    17581 aggctgcccg agccgcaagc tgggtcgtag attttgttta ctttctcctg cccgtgcacc
    17641 gccagccgcg caatcagctt ggatacgctt tgcggggtga aaaattcgcc gccggatttg
    17701 cctgcgttgg cagcgtagtt ggaaatcagg tattcgtagg catcgccgaa aaggtcgatg
    17761 tggtagtctt caaaactgcc gaaatcgagt tccgccacgc cttttaggac ggcggcaagg
    17821 cgtttgttct tgtcggcaac gatgctgccg agccggctgc tggtggtgtc gaagtcgtca
    17881 aacaggcctt taatgtcttg ttcggacgga tagccggagg cggagctttc aatcgcggta
    17941 aaaatttctt tcagcttggt gttgagctct tcgttttgat gggcttcggc ggcaatattg
    18001 caaaaaagct ggccggggta gatgaaatag cctttgactt tgacggcatc gtctttgatt
    18061 tcgggcgtga tgatgctgtc cggcatagcg gcgtaatcaa tactgctgtc gcctgcctgc
    18121 atatagtcgg taaagttttc gctgataaag cggtagaaaa gtgtgccgag aacgtattgt
    18181 ttaaagtccc agccgtccac cgcgccgcgt acttcgtcgg caattttcca aatttggcgg
    18241 tgcagttggg cgcgttgttg catttcggtc atcatcgaaa tccatataaa agttaaacaa
    18301 atcgaaatcg cctgatattt tcagacgatt ttttacgggc attcaaatta agccaaattt
    18361 tccagcatcc atttgacgta gcgctcgacg cctgctttga catcgaaaaa ttcttccgta
    18421 taacctgcct cgcgcaattt ggtgatgtcg gcttgggtga agctttggta tttgcctttg
    18481 agcgcgtcgg gaaatgggat gtagcggata aattcttctt ctaccaactc ttttaaactc
    18541 atttcaggtt tgccttcggc ggcgcggcag gcgttgacgg tggcagcggc gagttcgtta
    18601 aactgttggc tgcgacctgt tcctaggttg tagatgccgg aaagttcggg atggtcgaag
    18661 aagtagaggt tgactttggc gacgtcttcg acgctgacga agtcgcgggt ttgttcgccg
    18721 ttgccgtagc catcgttgct gccgaacagg ttgacgtaac cgtgttcgcg gtattggtgg
    18781 aaatggtgga aggcgacgga tgccatgcga cctttgtgtt gttcgtgttg tccgtaaaca
    18841 ttgaagtagc ggaagccgac gacttgggcg gtcagccttt ctttcatgcg gcggcgcaat
    18901 acttggtcaa acaggaattt ggaatagccg tacacattaa gcggtttttc cagttcgcgc
    18961 tcttcgcgga agatttcgcc tttgccgtaa accgccgcac tggaggcata aaggaaggga
    19021 atgcgttcgt cctgacacca gtccaacaaa tccagcgtgt actggtagtt gttttccatc
    19081 atatacaaac cgtcgtgatt catggtgtcg gaacacgcgc cttgatggaa aacggcttcg
    19141 atgttttgat aaggtaaaat gtgttccctc acttggcgga tgaattcatg tttgtcgaga
    19201 taatgggcga tttcgcactc ggcaaggttt ttgaattttt cgcctttgct caaattatcg
    19261 acggcaacaa tgtcagtaat accgcgttga ttaagtgctt tgacgatgtt gctgccgata
    19321 aagccggccg cgcctgttac gatgatggtc atattcggtt tcctttgttt tgagtaaaat
    19381 taataaactt agttacaaga aatcatcgat attgtcctga aacacaaatt tgcgatgata
    19441 ttccaaatat ttttctcttc cttcgggata tattctcatc ttattttttg catttggatt
    19501 gatattaagt ttttcccatg tgtaacggct tagtcgagta ccgcaaatca aacgaccatc
    19561 atccaagaaa gtaatataac cttgatcgaa caaccaatca taagtcggcg ttaaagctaa
    19621 cccatttaaa taatctaatg cctctttctc atttttctca gtaatacaaa ccatataagg
    19681 tttaatatga ctagccctta acaaaatctc atctgtaatt aatgtaaatg gacattgcgg
    19741 catatgattt ataacatcct tgcgatattt ctgctgccct tttctacttt tctgtttaat
    19801 gatttcttgc tctacgaatt gattaaattc tgaagccggt atttctaatt tttctcttga
    19861 taagagttgc gggtgcacga tagagcgaaa ttgataatcc aaaaatattc ggaaataaaa
    19921 taatggttct gaatcttcta tatctttgac aggcaatagt ttaagaattg ataaataact
    19981 aattttgggc aatacaatct tacgccaaat tgaccatata tcatcctccg aacggatata
    20041 acccctacgg taacctttag aattttccaa atggtctgat acatcatgga ttgaaaagaa
    20101 aatattttct gtaggcagat tattaatact taaaacgtaa tcatcataaa accttgaaat
    20161 atcatgatga tatttctgtt cttgtttgtg atattcaact tttgcatcaa ccaaataacg
    20221 ctgtaaattt ttcttattaa aaaaacaatt tttctgaatg agaggatatt ctaccctcct
    20281 tccatttttt gtctcagtaa tcccccaatt ttcatactca aaaaatgtac ttagttttcg
    20341 accactatca tcaatataac tacctacata tttgcgtgct tccccattac caccaaaaac
    20401 agataacttg ttattcctgt gaataaaact atcttccgca cgtaaatttt gaatactatc
    20461 gacaataaaa tactctatgt tttcaatagt aattctttga aaatccggca ttttactctc
    20521 cataaagcgt aatatccatt tgttttaata tagcgattaa tacatcaaca acaatactgt
    20581 ttcccgcttg gcgatacatt tgagtatcac taactaaaat tttaaaatca tctctaaaac
    20641 ccattagccg caaacattcc ctaggtgtta atttacgaat acgtccacga ttatgagtaa
    20701 cataattatc aaccccggca cgatgcattt tatgcatagt ttgcaataat gggcgagcta
    20761 caggcaaatc agtttccgta ctggttttaa aattttttgt tcctcctgcc aaaacataat
    20821 ttttgatttt ttcagaaaga taatattttt cctcaacgtt atttacatca aaaataaagt
    20881 catcaaattc agattcgcgg gctgcctgaa aaataaaatc accatgccaa ttaaattgtt
    20941 gatttgcttt ttggcataag gcaatttctc cattaatctg tgtataacgt ttttgtctat
    21001 ttttagaact ggtaacaaat ttcacaccct tttcacgtaa aaaatatttg ctatcagtat
    21061 agtcctccaa aaaatcttgc atagtatgtt ctaattcaat cttttcagga aactgaaaac
    21121 catttatagg aggggtatga aagccaacaa caaaaatacg ctcacgatgt tgaggaatcc
    21181 cataatcctt actattcatt atttggaaat ataagtcata accaagtgaa taaaaaacac
    21241 tttttacaac tttccaagtt tttccattat catgattgag caagcccttt acattttcat
    21301 aaataaatat ctttggttgg acttcatcca caacgcgagc aaattcatag aataatgttc
    21361 ctcgtgtatc ttctaatcct gcacgtttgc caaccatgga aaatgcttgg caaggactgc
    21421 ctccaactaa aatatcaact tgatttctaa actttctcgc atcaaattga gtaatgtcgt
    21481 tatgccaaaa atcttcattt aatttatagt ttccaagata acttttttta acgtatggat
    21541 caatatctcc tgcaaaaaca atggtatggt ttaaatttaa tcggtggaat gcctgttcaa
    21601 ccgcaccaat cccgctgaat acggttgcta atctaatatg tgaatcaggt ttaagaaaag
    21661 ttttagattt ccaaccttgt tgactgggaa agagcaaagt tttttgtaat cgagtatcgt
    21721 gtgtctgtgc cattgtcgaa atagtcatac ttatatcgtt ctgtttatct tatcaatatg
    21781 aaaactacat tgttgattgc cctgacaatg ccttgatcaa ttcggcaaac gagcaaaccg
    21841 ccgtaccgag tttcgccacg acaaccccgg ccgcagtatt ggcaaggtac atggcttcgg
    21901 gcatggtgca gcctgccgcc aaacccaagc ccattccggc aatgacggtg tcgcccgcac
    21961 cggatacgtc gtaaacttct tgggcgcggg tgggctggta aatcggttcg ccttcgctga
    22021 acaaggtcat gccttcttcg cttcgggtca gtaaaacggc ggtcaggtcg aggtggcggc
    22081 gcaggttttg cgctttttcg gtcagctcgc tttcgttttt ccaactgccg accacttctt
    22141 tcaattcggc gcggttaggc gtaatcagag ttgcaccgac atatttttcg taatcgtcgc
    22201 ctttggggtc gattaagacg gttttgccgg cgtgtttcgc ccaatcgatc atatcggaga
    22261 tatgcgacag gccgcctttg ccgtagtctg aaaaaatgat tgcgtcgtat tcgggcaaga
    22321 tttcgcggta tttctgcttg atttgttcca acacttcgca gttgggatgt tcttcaaaat
    22381 caagacggat aagctgctgg ttgcgggcga cgacgcgcag tttgacggtg gtggcgattt
    22441 gtttgtcgcg catcagatag gaggcgacgc cgtcctgcac catcagcgca tcgagcgcgt
    22501 cggcggcttc gtcgttgccg gttacggaca acagccctgc cctgccgccc aacgaagcga
    22561 tgttgcgcgc gacatttgcc gctccgcccg cgcgttggtc gattcgtccg attttcgcca
    22621 ccggcacggg ggcttcgggc gaaatacggg acacatcgcc gaaccaatag cggtcgagca
    22681 tcacgtcgcc gacaaccagg actttggctt gcgcgaaacg ggatttgagg gtttcttgtt
    22741 ggaacttggc ggacatattg cctccgttgt attttttcag acggcctcag cctgtaaggc
    22801 cgtctgaaaa tcagtttgct tcaaggtttg ccacgcggtt cacttcgcgc aatacggcta
    22861 ccggatcggc agcttgggtt acaggacgac ccattaccaa ataagtcgaa gcggcagcca
    22921 aggcttcggc cggtgtcatg atgcggcgct ggtcatcatt attgccggca acgtccaagc
    22981 ggatgccggg cgtgaccaag acaaaatctt gtcccaactc gcggcgcagc ggtgcggctt
    23041 cttgggcgga acagaccacg ccgtccaagc ccgaactttg cgccagtttt gccaagcgga
    23101 tgacttgttc ttcaggggcg gtgttcaaac cgatttccgc caaatcgctt tgttccatgc
    23161 tggtcaacac ggttacgccg attaagagcg gctttgtacc gtatccggca acggcttctg
    23221 ctgcggcttc catcatacgg cggccgcccg atgcgtgcat atcgaccatc caaacgccca
    23281 tatcggcagc gactttgcag gcttgcgcga cggtgtgggg aatatcgtgg tatttcaaat
    23341 cgaggaaaag tttgaaacct tgattgatta agctttctgc caaattgcgt cccgtcgcgg
    23401 taaacagctc tttgccgatt ttgatttgac acaatgtcgg gtcaaggttg cggacgaatc
    23461 cgagcgtgtc tttttcgttg gaaaaatcaa gggcgacgat gacgggggtg cgttgttgcg
    23521 gagtttggaa gtcggagatt aaaggattca tggtgttttc gtttcgggtt ttcagacgac
    23581 ctttatcgtc tttttcgcag tatagtggat taactttaaa ccggtacggc gttgtctcgc
    23641 cttagttcaa agagaacgct tctctaaggt gctgaagcac aaagtgaatc ggttccgtac
    23701 tatctgtact gcctgcggct tcgtcgcctt gtcctgattt ttgttaatcc actataacaa
    23761 cgtaaattca aaccgtaggt cgggcattga tgtccgacct acgcggactc aagcctccga
    23821 caaactgttt cggataaatt tgaaccggta catcagccgt cattcccgcg caggcgggaa
    23881 tccattgata aaactcagtt catcagattt aaacagcgat tgcccaaatt cagaaatgga
    23941 ttcccgcctg cgcgggaatg acgccaaatg attatttttc ggttgcaaca cgctgtttat
    24001 caaaaacagg tcgtctgaaa acctgatttt ctgttttcag agtgcgaagc ctgcccgtgg
    24061 gtacgacctt ttcgatttat ttgcccaatg cgcccaatac ttcggctttc acggcttcta
    24121 ccggctgagt gccgtcaact ttgatgtatt taggcgcgtg ttcgccttcc agtttgctgt
    24181 aaaaatcgac caaaacttcg gtttgctcgt ggtaaacggc aaggcgtttt ttcacggttt
    24241 cttctttgtc gtcgtcgcgc tgaatcaaat cttcgccggt tacgtcgtct ttgccttcaa
    24301 ctttgggcgg gttgtaggta acgtggtaag tacggccgga agccaaatgc acgcggcggc
    24361 cgctcatacg gtcgacaatc acgctgtcag gcacgtcgat ttcaacgact gcatccaaat
    24421 ccacgcctgc ttcaaccatc gcttcggctt gtgccaatgt gcgcgggaaa ccgtcgaaca
    24481 ggaaaccgtt tttgcagtcg tcttgcgcga tgcgttcttt gaccatgccg ataatgatgt
    24541 cgtcgcgcac caagccgcct tcgtcaatga tttttttcgc ttccaaaccc aagggcgtgc
    24601 cagccttaat cgcggcgcgg agcatgtcgc cggtagagat ttgcggaatg ccgaacgcgg
    24661 cggtgatgaa ttgcgcctga gtgcctttgc ccgcgcccgg cgcgcctaaa agtaatgctt
    24721 tcatgaagtg atcctttgaa atgtgtttat tggaatcaaa cagatggttt acgattctac
    24781 acggcttggc acaatagttc aaaaaccaac tgcacaaact gccatttgca agcaaatgtt
    24841 ttgcatacct ttgccgagtc tgatattcac aagccggcaa ctttttggga caatccccaa
    24901 aatttcaaaa catcacaccc taccaaaaag gaacatccgt gaaacgaatt tttctgtttt
    24961 tggctaccaa tatcgctgtt ttggtcgtaa tcaacattgt tttggcggtt ttgggcatca
    25021 acagccaggg cggcacgggc agcctgttgg cgtattccgc cgtcgtcggc ttcactggtt
    25081 cgattatttc gctgctgatg tccaaattca tcgccaaaca atcggtcggc gcggaagtta
    25141 tcgacacgcc gcgcaccgaa gaagaagcct ggcttttgaa cactgtcgaa gcccaagcgc
    25201 ggcaatggaa cctgaaaacg cccgaagtcg ccatctacca ctcccccgaa cccaatgcct
    25261 ttgccacggg cgcatcgaga aacagctccc tgatcgccgt cagcaccggt ttgctcgacc
    25321 atatgacgcg tgacgaagtg gaagccgtat tggcgcacga aatggcacac gtcggcaacg
    25381 gcgatatggt tacgctgacg ctgattcaag gcgtggtcaa tacctttgtc gtgttcctgt
    25441 cgcgcattat tgccaacctg attgcccgaa acaacgacgg cagccagtcc cagggaactt
    25501 atttcctggt cagcatggta ttccaaatcc tgttcggctt ccttgccagc ttaattgtca
    25561 tgtggttcag ccgacaacgc gaataccgcg ccgacgcggg cgcggcaaaa ctggtcggcg
    25621 cgccgaaaat gatttccgcc ctgcaaaggc ttaaaggcaa cccggtcgat ttgcccgaag
    25681 aaatgaacgc aatgggcatc gccggagata cgcgcgactc cctgctcagc acccaccctt
    25741 cgctggacaa ccgaatcgcc cgcctcaaat cgctttaaac cgatttgaaa cggcaaaaac
    25801 cgtacgcgca agcagtacgg ttttttgtat atgctgtctg aaaacatcag gtttccatac
    25861 cgacaccaac cgtatccatt tccgcaccgg atacgccgca ttgatatacc ctgataaacc
    25921 gtatccggac agaaaatata gtggattaac aaaaaccagt acggcgttgc ctcgccttag
    25981 ctcaaagaaa acgattctct aaggtgctga agcaccaagt gaatcggttc cgtactattt
    26041 atactgtctg cggcttcgtc gccttgtcct gatttttgtt aatcccctat atcagataaa
    26101 acagcggtaa aattgccgtc tgaaaccaac tctgcgccat acgggacacg ctctgcacgt
    26161 ctatttgcct ccgatatatc ctccctactg taggaacgtc tgccgatttg cacgaaatcg
    26221 gcacctctag cacccttcag gatggcggac gttatccgtt cttgggattc tgcaagacgg
    26281 tcgacggcat tatacagacg ctcggtctga tacccttccg aagcaacctg cttcaggtat
    26341 tccaaataac cgtaacccaa cgcccctccg acaagcaggg tcgtcaacgc taccagcgtt
    26401 gccgaacgga ttttaggggg catatcttga agcatatctc taccgaaccg aaccaaagtc
    26461 tctttaaagt cggactctac acgcgtagaa ccctcggaaa tcttgaagtt caactcatac
    26521 tgcttccgtt tgtccgctgt caaccgcttg aggttgtcat ccccgtattg gacggttgac
    26581 acaaccctgt acagactgtc ctgtatttcg accagcgtct gtgcaaagtt ggcggtcata
    26641 actggattcc caacccttgc cgccgattct gatactcaaa acggtatcca ggcgttccag
    26701 cagggcttcg ggagacagca gtgcgtattc ttcaatattt tccataggtt gattatagca
    26761 gaaccttata cctgttacag acaaggttgt cgggatttga acgtccgtcg agcttggttc
    26821 gtaggattta gggtgttgtg aaccttacct atcgtaccca agcactttgt acctgtttga
    26881 aataaggttg tcgggcgacg ttcgaccctt gagcctgtga ttgatttgtt ggaaatgtac
    26941 acctaaagca gcacctgcgg cagtaacgct tgggaacgtg agtgtttcgt tggtcgttgt
    27001 gtcgaggatg aggaggggct tccctacatt tggaggtgtt tgggtttttg acatcttagc
    27061 acactcagga cacccgtact ttgactttat aagggcatta aatgtcgaat aacgaacgtt
    27121 cccgtgcata ggacaattaa cggttactgg gtaattcaca ccgtcaaagt cggttagaga
    27181 gatattaggg aatatatcag taagttcaga agctgcttgg ttgaaagatt tggccatttg
    27241 agactccaaa attgattgtc aaatggattt aatagcaaat ttataaaaat gtcaaatttt
    27301 tcgagaagca cctgatagtg gcgaggggta tacactgtta aaaagtgtat acgtcattta
    27361 gtgcagatgt gaaccttata cctccctgca agtaaagtct ctgtgtgaac ccgacctttt
    27421 atacggtcag tgatagctgc tgggtttata cccaatgacc ttgcagcaga tgctcttgag
    27481 gggaatgtaa gtgtttcgcc agttacagta tcctccaata ctgtattttt agataattta
    27541 tctattgtat cttctggaat tttgtgggaa ttgacaccat aagatgcaca ttttggacac
    27601 ccacttttag agtccaacat acttttgaat gtagagcaag taaccctccc gtgtatagga
    27661 cagacgatta ccgtcgggta acgaacgcca ttgaactcca caagattcaa atgtggaaat
    27721 ttagaggtta gcttagaaac tgcttgagta aatgtaagtg ccatagtggt tgtgaactta
    27781 ctttaccaac ctataacgat acctaatcaa tcggtcaaac ggagtgcgtc cgcttaaacg
    27841 tacagctaaa gtgctgttgg agcaaccgat aaatttactg gcagccagtg tacttgcaaa
    27901 ctcgtagatt tcccctgtct ctgtgtcctc caacctaatg gcaacggcgt ttggagaggg
    27961 cttttcggga tttcgagtac attcaggaca tcctgttgag gatttcatgg cagcttgtag
    28021 attcccgtaa acaaccgacc catgcaaagg gcattggatt gttaccggtt cgtagttttt
    28081 agtgaacttc aaaaccgtct tatcaggaaa tttactggaa aaccttaact gaacatcttg
    28141 tagcgagagt gattttggca ttatagacct ttctaaaatg aaagcattat acaaccaaaa
    28201 tatagaaatt ttcaaatttt ctctgaacgc actggtatcc gatggagcta aatgtaaaga
    28261 aaaatggtat acgtctttac taaatcttta cacattttaa ctgtatagtg tttgacaaac
    28321 aacaggcata ggcgcataat gcacccgtag ttagatagat agtaaggaga taaccaatga
    28381 cttagcacct gcccccctct ctctcgcgca ccccgcccct cgcttgcggc taatgggctt
    28441 tgcgcgacat atacactttc atctcctgca tggcttaact gcctatgcaa tagtacacac
    28501 atcaacaaat caggctggca cgtcttggca ttggctgacg taccattgta gcccgaaatg
    28561 ttgataccct actatttatg accaacggcg gattagggct tcagacggca attttactgc
    28621 aaaaccttat gcgaacggat gatgcagcac aatcgtttct tcgcggtcgg ggccggtgga
    28681 gacgatggcg accggcgcac cgcagacttc ttcaatccgt ttcaaatatg ctttggcatt
    28741 ggcaggtaat gcgtcgtagc ttttcacgcc gaaagtggat tcgctccaac cgggcatggt
    28801 ttcgtaaatc ggcttgcagg cttctaccgc atcagagccg caaggcagga tgtcggtttt
    28861 gccgccgtcg ggcaattcgt agccgacgca gatattgatg gtttcaacgc cgtccattac
    28921 atcgagtttg gtaatacaca taccggaaat gccgttgatt tggatggagc gtttcagggc
    28981 ggcggcatca aaccagccgc agcggcgtgc gcgtccggtt accgaaccga attcgtgtcc
    29041 gcgttctgcc aaacctgcgc cgacttcgtc gaacaattcg gtcgggaacg ggcccgaacc
    29101 gacgcgcgtg gtataggctt tgacgatgcc caaaacataa tccagcattt gagggcctac
    29161 gcccgcgcct gccgaagccg cgccggcgag gcagttggac gaagtaacga aggggtaagt
    29221 gccgtagtcg atgtccaaca acgcaccttg cgcgccttca aacagcagtt tttcgccgtt
    29281 tttgtttttt tcgttcaaca cgcgggacac gtcggtaatc atcggcgtaa tgcgcggcgc
    29341 gactttttcg ataaccgcca tcacgtcttc cgctttcacc gactcggcat tgtgcagatg
    29401 ttgcagttgg acgttgtaat aagcaaggac ggcatccagt ttttcacgca gtttttcagg
    29461 atgcagcaaa tcggcggcgc gaatggcgcg gcgtgccact ttgtcttcgt aggcagggcc
    29521 gatgccgcgg ccggtcgtgc cgattttgcc tttgccgcgc gatgcttctc gggcttggtc
    29581 gagcgcgatg tgataaggca ggatcagcgg gcaggtcggc gcgattttca gacggccttc
    29641 gacgtttttc acgcctgccg cgttcaactc gtcgatttcg cccaacaggg cttcggggga
    29701 gacgacaacg cccgaaccga tgaagcagtc cagactttca tgcaggatgc cgctcggaat
    29761 caggcgcaaa atggtttttt tgccgccgac aaccaaggta tgacccgcat tgtggccgcc
    29821 ttggaagcgc accacgcctc cggcttcttc cgccagccag tcaacgattt tacctttacc
    29881 ctcgtcgccc cactgtgcgc cgattactac aacattttta gccatagcca tataaccttc
    29941 gatattaaaa attattgcgg aaatccccaa accggtgcgg ctttgcgccc cgccacgatt
    30001 tgcctttcat ccgccatgaa cgggtattta gcgtttcacg acctgccaaa cgccgtccgt
    30061 ctttttcaga cggcctgcaa gctcttccga aacattgtgt ccgataccgt aatcgattac
    30121 gacacactgc ccttgttcac gcaaggcttc gaccgcttcg tgcgccgctt cggcatcttc
    30181 cgcatcgacc aacacggcgg gctgccgttc gatggcgggc aaacgcccga taaagctgcg
    30241 caagtcgaaa ctgaatcccg ttgccgggcg cgccctgccg aaatatccgc ccaatccgtc
    30301 ataacgcccg ccgcgcgcga ccgcgtcgtg gaaatcggcg gcataggcgg catacagcaa
    30361 gcccgtgtgg taattgtcga cgcgcagctc ggacaagtcg atatggattt cacaatcggg
    30421 gaatgcgtcg cacaccgcct gcaattcgcc caacgcgccg ccgaccgccg acaaatccgg
    30481 caaccgtccg cgcgcgtcgg acaacacttc acgcccgccg tacaggcgcg gcagcagcga
    30541 gaatgctttt gcccacatac cgtccagctt ccaagccttg acctgcgctt cgaccgtctc
    30601 ggtatcttta tcctgcatca aggcaagcag cgttgcagac tgccccgcat ccaaatgcgc
    30661 cgcatcggac aaggcgcgaa atatgccgat atgccccagc gaaagcagca ctttgcccat
    30721 atcggcaatt ttcatgcttt tcagcatcag gtctatcagc tcgatgtcgc cacggatgtc
    30781 ggcaaaaccg tacatttccg cccctgcctg caagggttcg cgcatattca gcagaccgtc
    30841 ggactgcgcg tgcaacaccg gaccggcata acacaaccgg ttaatccctt ggttggcgga
    30901 taaaagatgg gcatcgatac gcgccacctg cggcgtgatg tcggcgcgta tgcccaactg
    30961 cctgccgctg agcctgtccg ttaccaaaat ggttttcagg gaaagccccg catcgatatg
    31021 cgtcagcagg gaatgtgcgt actccatcag cggaggctgt accagttcat aaccgtgtac
    31081 gcggaacagt gccaacaact gctccctcgc gctttcaagc tgccgcgcgt tcgtgggcag
    31141 tacgtcggcg atatgttccg gaagctgcca tgtctgcatg agaatctgcc ttctatttta
    31201 tcatgtcaat acaaaaggct gcccgatccg caatcagacg gcatctttgc cgcaacgcat
    31261 cacgccgctg tcaaaacggc gatgcttcct tccgattcag aatccgaaca gcccctgtcc
    31321 gacggctcaa tgccgtcaag tcgttaaaac caaactttac cataaaatac acacaatctg
    31381 aatatacttt tataccctgt atccgccgtc tgacggcacg gcattttttt actatataat
    31441 gtgcaccatt atttctcatc acttcctgct gccgttttct gcttcagacg gcatttttgt
    31501 tgattgaaca cttatgctgc tcgacctcaa ccgcttttcc ttttccgtct tcctgaaaga
    31561 agtccgcctg ctgaccgccc ttgccctgcc catgctgttg gcgcaggtcg cgcaggtggg
    31621 catcggtttt atcgatactg tgatggcggg tggtgcaggc aaggaagact tggcggcggt
    31681 ggctttgggc agcagcgcgt ttgccacggt ttatattacc tttatgggca ttatggcggc
    31741 gctgaacccg atgattgccc agctttacgg cgcgggtaaa accgacgaag tgggcgaaac
    31801 ggggcggcag gggatttggt tcgggctgtt tttgggcgtg ttcggcacgg tattgatgtg
    31861 ggcggcgatt acgccgttcc gcaactggct gaccttgagc gattatgtgg aaggcacaat
    31921 ggcgcagtat atgctgttca ccagcttggc gatgccggcg gcaatggtac accgcgcact
    31981 gcacgcctac gcctccagcc tgaaccgccc gcgcctgatt atgttggtca gctttgcggc
    32041 gtttgtgttg aacgtgccgc tgaactatat tttcgtttac ggcaaattcg gtatgcccgc
    32101 tttgggcggc gcaggctgcg gactggcgac gatggcagtg ttgtggttca gcgcgctggc
    32161 attgtggatt tatatcgcca aggaaaattt cttccgccca ttcggactga cggcgaaatt
    32221 cggcaaaccg gattgggcgg tgttcaaaca gatttggaaa atcggcgcac ccatcgggct
    32281 gtcttatttt ttggaagcca gcgcgttttc gtttatcgtg tttttgattg cgcctttcgg
    32341 cgaggattat gtggcggcgc agcaggtcgg catcagtttg tcggggattc tctatatgat
    32401 tccgcaaagc gtcggctcgg cggggacggt gcgcatcggc ttttcgcttg ggcggcgcga
    32461 attttcgcgg gcgcgttata tttcgggcgt gtcactggtg ttaggatgga tgctcgccgt
    32521 gattaccgtg ctttccttgg tattattccg ttcgccgctg gtaagtatgt acaacaatga
    32581 tccggcggtt ttaagcatcg ccgccaccgt cttactgttc gccggcttgt tccaaccggc
    32641 agacttcacc caatgtatcg cctcctacgc cttgcgcggc tacaaagtta caaaggtgcc
    32701 gatgttcatc cacgccgccg ccttttgggg ctgcggtctg ctgccgggct acctgctcgc
    32761 ctaccgtttc gatatgggca tttacggctt ctggacggca ttgattgcct cgctcaccat
    32821 cgccgccatc gccttggtgt ggtgcttgga attgtgcagt agggagatgg tcagatcgca
    32881 taaggctgtc tgaaaactcc gccgattaaa aatatagtgg attaacaaaa accagtacgg
    32941 cgttacctcg ccttgccgta ctatttgtac tgtctgcggc ttcgttgcct tgtcctgatt
    33001 tttgttaatc cactatagaa acgatgggct gaagcccgcc actggcaata ctcattttaa
    33061 agttcaaacc catgcaaccc atccgatacc gaaccgacct taccccctac aacaccttcg
    33121 gtcttcgcgc ccaagcccga gcctttatcg cgctcgaaca tgccgacgag ttgtgcgaca
    33181 tcgtccgact gcccgagttc gaccgcgata ctgttttatg gctgggcggc ggcagcaata
    33241 tccttttgat ggaggactac gccggtctgg tcgtacatat ggaaaacaaa ggcatacgcg
    33301 aaattgctcg ttcagacggc atggttctga ttgaagcgca ggcgggcgaa atctggcacg
    33361 attttgtcct gcacaccgtc gcgctgggtt tgaacggttt ggaaaacctg agcctgattc
    33421 cgggtacggt cggcgcgtcg cccgtgcaga acatcggcgc atacggcgta gaggcgaaag
    33481 atgtgattca cagcgtgcgc tgcttcgatt tggatacgga aacctttgta accctttcca
    33541 atgccgactg ccgcttcgcc taccgcgaaa gcctgttcaa gcaggagggt aaagggcgtt
    33601 atgtgattgt ttcggtcgta tttgcattaa aaacgcattt tgtgccgact ttgggttacg
    33661 gcgatttggc ggccgccgtt gccgaactga gcgcgggcag ggtcccgacg gcgaaagatg
    33721 tttccgatgc agtgtgtgca atccgcaaca gtaaacttcc taatcctaac gtgcttggca
    33781 atgtcggcag tttctttaaa aaccccgtcg tcagcgcaga aaaagccgcc accttgttgc
    33841 agcggcatcc tgatatgccg cattatccgc agccggacgg ttcggtcaaa ctcgccgccg
    33901 gctggctgat cgaccaatgc cgtctgaaag gcttccaaat cggcggcgcg gcggtacacg
    33961 acaaacaggc attggttttg gtaaacaaaa acaacgcctc ggcaaacgat gtccggcagt
    34021 tggcgcaaca catcaaattt acagtatttg ctcggtttca ggtagaattg cacgccgaac
    34081 ccaattggct gcctgcttcg ttcagcctgt aaatccaagg agtatgcccg tgacccgccc
    34141 cgccaaaatc aatacttaca cacgcatcat cgacgccagc cttgcgcttt tcaacgagga
    34201 aggcgagcgc aacatcagca ccaaccatat tgccgcccac ttgggcatca gtccgggcaa
    34261 tctctattac cacttccgca acaaagacga aatcatcgtc caactgttca aacgttacag
    34321 cgaagccctg ctggaatacc tcaacgaagc cgtgttgccg tctgatgtgg aagactccat
    34381 caactatatg gcgggcattt acgacgtgat gtgggaatac cgcttcctat tcagcgacgt
    34441 gaacaccctg cttgcgcgca gtgctgaatt attgggcgaa cacaatacct tcacccaagc
    34501 caaagtctcc ccgctcttgg tcaacctgct tacccagctc aacggcttga acatcatcca
    34561 agccgaccaa accgctatga acgacctcgc cgtcaatatg tggatggtca cgaaatactg
    34621 gttcgacttc gacagctccc tgcgcggccg caccaagctg accgaagact ccaaagcacg
    34681 cggcgtccgt cgtaccttaa gcctccttcg tccttatctt ttgcccgaac accgcgagaa
    34741 atacgaccgg aaaatcggca acggcaaccc gtaaacccac agcgtccgaa tcagccttca
    34801 gacggcactt acccgacggt atcagcgaat gcgcgcagtc cccccgcccc atatctccat
    34861 cacaggccta ggttacctcg gcctgccgct ggcacaaaag ttttaccaac acggcagccg
    34921 cgtcgccgcc ataaaacgca gcctgacttc ggacgacatc aacctgccca tacatctcga
    34981 caccttcgac ctcggcagca ccgatgcctt tcagacggca ttgtggcggc accacgccga
    35041 caaacccgtc tggttcttcc tgctaccgcc ctcctcgctg acgcattacg ccgacaccgt
    35101 caaacaatgg gcagaacttg cccgggcgtg caacgtgcaa cacctgattt tcacaagcag
    35161 caccagcgtt tacggcgata cagcgcgcga atgcgacgaa accgccacac ccgatccgca
    35221 aaccgagtcc gcccgccaaa tcctcgccgc cgaacaacac ctgctcgaca gcggcatctc
    35281 caacatcgac atcctgcgac ttggtgggct ttattgcgcc gaacgccatc ccgtcagccg
    35341 tctcgttcaa aagcaaaaca tcccaggcgg caaccgcccc atcaacatcg tccaccgtaa
    35401 tatcgccgtc gaaagcctgt ttcagacggc atttaacccc ggcggcaggc ggctgaaaaa
    35461 cattatcgaa ccgcgccacc cgacacgacg cgaattctat acggaagaag ccgccaaact
    35521 cggcttgccg gctcctgatt tcgcacccga cgacagcgtg ggcaaaatca tccgtaccgt
    35581 ttgcgataac ggcttaagcc tgtaaaataa gcgtcaacag caacaaacag gcattctccg
    35641 ccccgatcag aaaatatgtc cgccctcctc cccatcatca accgcctgat tctgcaaagc
    35701 ccggacagcc gctcggaact tgccgccttc gcaggcaaaa cactgaccct gaacattgcc
    35761 gggttgaaac tggcgggacg catcacggaa gacggtttgc tctcggcggg aaacggcttt
    35821 gcagacaccg aaatcacctt ccgcaacagc gcggtacaga aaatcctcca aggcggcgaa
    35881 cccggggcgg gcgacatcgg gctcgaaggc gacctcatcc tcggcatcgc ggtactgtcc
    35941 ctgctcggca gcctgcgttc ccgcgcatcg gacgaattgg cacggatttt cggcacgcag
    36001 gcagacatcg gcagccgtgc cgccgacatc ggacacggca tcaaacaaat cggcaggaac
    36061 atcgccgaac aaatcggcag gaacatcgcc gaacaaatcg gcggattttc ccgcgaatcc
    36121 gagtccgcaa acatcggcaa cgaagccctt gccgactgcc tcgacgaaat aagcagactg
    36181 cgcgacggcg tggtacgcct caacgaacgc ctcgaccggc tcgaacgcga tatttggata
    36241 gactaacctt cagacggcat ccgacatgaa cagccctttt cacaacatcg gcatcgtaac
    36301 ccgccccaac acgcccgaca tccaagacac cgcgcacacg ctgattacct ttttgaagca
    36361 gcacggcttt accgtctatc tcgacgaagt cggcataaag gaaggctgca tctataccca
    36421 agacaccgtc ggctgccata tcgtcaacaa gaccgaactg gggcaatact gcgacctggt
    36481 cgccgtttta ggcggagacg gcacctttct ctccgtcgcc cgcgaaatcg ccctgcgcgc
    36541 cgttccgatt atcggcatca accaagggca tttgggcttc ctgacccaaa ttccccgcga
    36601 atatatgacg gacaagctat tgcccgtttt agaagggaaa taccttgccg aagagcgcat
    36661 cctgattgag gccgcactca tccgcgaagg caaaaccgcc gaacgcgcca tcgccctcaa
    36721 cgatgccgtc ctctcccgtg gcggcgctgg acagatgatt gagtttgaag tcttcgtcaa
    36781 tcgggaattc gtctataccc agcgttcgga cgggctgatt gtctccaccc ccaccggatc
    36841 gaccgcctat tcgcttgccg ccggcggccc catcatgcag gcaggattac acgccttcac
    36901 actcgtaccc atctgcccgc aatccatgac caaccgcccc atcgccattc ccgacacgtc
    36961 tgaaatcgaa atcctcgtta cccaaggcgg cgacgcgcgc gtccatttcg acggtcaaac
    37021 ccatatcgac gtgcaaaacc tcgaccgcat caccatccgc cgctaccgaa atcccttacg
    37081 cattttgcac ccgaccgact accaatattt caaaaccctg cgccaaaaac tgcactgggg
    37141 cgaacaattg gtctgaaccg acccttaccg gaaaacacct atgaatccaa aaactccccc
    37201 cgaccataca gaagatgccg tgcgcctctc caaacgcatg gcgcaactgg ggctttgttc
    37261 gcgccgcgaa gccgacggct acatcgaaca gggctgggta acggtcaacg gcaaaaccgc
    37321 cgtactcggt cagaaagttt caccggcaga ccgtatcgaa ctgaacaaga aagcccacga
    37381 acagcaggcg gcacgcatta ccatcctgtt gaacaaaccc gtcggctatg tcagcgcaca
    37441 agcggaaaaa ggctataaat ccgccgccga actgattacc cctgaaaatc actgggaagg
    37501 cgataccggc cgcatccgtt tcgatccgaa acacaaaatc ggcctcgccc ccgccggcag
    37561 gctggacatc gactcggtcg gattgctggt attgactcag gacggccgta tcgccaagca
    37621 gcttatcggc gaaaacagcg gcagtgaaaa agaatatttg gtgcgcgtgc gcggcaaatt
    37681 ggacgaaaaa ggacttgcct tactgaatca cggattgagt ttggacggcg agaaactgcg
    37741 tcccgcccaa gtagaatggc aaaacgaaga ccaactgcgt ttcgtattga aacaaggtaa
    37801 aaagcggcaa atccgccgta tgtgcgaact ggtcggactg cgcgtcgtcg ggctgaaacg
    37861 catccgcatg ggtaaggtca agctcggcag gctgccgccc ggcaaatggc gttatcttgc
    37921 tcccggcgaa tcgttttaaa taagcattaa aaaatgccgt ctgaacgtcc ttacgttttc
    37981 agacggcatt gccttatgcc caaataacgg tttcttccaa gggcctgcgc gctttcgtgg
    38041 cgatttcctg aacgcggtag ccgaatgtcg cggcaacgga cacgccccat tctgccgcat
    38101 cgaacaaacc gaactgcccg gacaatacgc gttccatatc ggcatagttg aaaccttcca
    38161 ccgggcagga atcgatacct gccatcgccg cacccgtcat catgttgccc aacgcgatat
    38221 aggtctggcg gcaacaccag tcaaacaagg cgcgcgaatc gtccaaaatc ttgatgtcgt
    38281 cggcttgaaa cgcctgatac ctcgccaaag attttgccat ggcatccggt tcggtaacgc
    38341 cgcgccgttt gaggctttcc aacataaacg ggctgtcgaa gcgggcattt ttctttgcca
    38401 aaaacaccac caaatgactg gcggtatcca aagcatccgc cataccccaa gaaaacggct
    38461 tgattgcctg tcggatttcg gggttttgaa ccacaacaaa ctgccaaggc tccgaaccga
    38521 ccgaactggg cgacaaacgc ccgagttcta aaataaactg aaaatcctcg gcactgattt
    38581 tacgtgccgc atcgtaatgc cggcatgatt tgcggttttt aaatgcggat agaacctgct
    38641 ctttgcttaa tactgccatc gcacatcctt tcattacttg tattgcaaat tgatttcaaa
    38701 ccgatacggc actgccgcac catacaatcc gtcctcgcca tcaagcttca tccggacttt
    38761 acccatctct ctacggctgg gaaaacaaag cggcggcaga ctcgggaata acgccccaat
    38821 gcctttcaga cggcatttgg gcatcattgc aaacgcgcct acgggtaggc gatataggca
    38881 taagccgaat ggttgtggat agactcgaaa ttctcgctct cgacaacgaa actcttgatg
    38941 cgtttgtcgg cgatcagcga agtagcgaca tcgcgcacca tatcttccac gaatttcggg
    39001 ttttcgtagg ccttttcggt aacgtatttt tcatcggggc gtttgagcag gccgtagagt
    39061 tggcagctcg cctgcgcctc cacataatcg atgacttcct cgataccgac ttcggcatcg
    39121 gcagtcaggc tgacggtaac gtgcgaacgc tgattatgcg cgccgtattg ggaaatttct
    39181 ttggaacacg ggcaaagcga ggttacggga atcatgacct tcatactgtg gccgtatgcc
    39241 ccgtctttga tttcgcccgt gagacagaca tcataatcca gtaaggaccg aataccggaa
    39301 accggcgcgg ttttcttgcg gaaaaacgga aaagaaacgc tgattttgcc ggcgcgggaa
    39361 tctaaaagcg cgaccatttc ggtagtcagc ttgcgcaatt gtgcaaaatc caaggcttcg
    39421 gcatgttgct ccatcaatgc gacaaaacgc gacatatgcg tccccttctg ctcagcaggc
    39481 aggtaaaccg tcatggtcag acgggcaatg gtggactgga tgccttctgc agtttgcagg
    39541 gtaatcggaa agcgcaggtc tttgataccg acctgattga tcggcagatt gcgcaaatct
    39601 cggctggatt gcacgtctgc aatagtgttc atgagttgtt tttccttaat attcatgtaa
    39661 ttgttgagat gtgtgcatgg tatggcgttt ccgccgggga atataccgtc aatctgcaaa
    39721 tgcgagatta tatcactcgc ttttagcagc tgcattgatt gtttctattt ttcagatagt
    39781 gataaaatct tcgattattc acatctacat aggcagagac caaatgaaat tcgccacgaa
    39841 agccattcat tccagctacg attgcgacga acacaaccgc gcgctgatgc cgccgattta
    39901 tcaaaacagt atgtttgcgt tgcacgagat tggcgaaaat gtgccttacc gttattcgcg
    39961 cctgagcaac ccgacccgtc agattttaga agacaccgtt gccgatttgg aacacggtgc
    40021 ggcaggtttt gcgttttcca gcggtatggc gggaattgat gccgtatggc gcactttcct
    40081 gcgcccgggc gataccattg tcgccgtcgc cgatatttac ggcggcgctt atgatttatt
    40141 ggtcgatgtt tatcaaaaat ggggggtgaa cgttgttttt gccgatttag gcaatccgga
    40201 taatttggac gaactgctta aagcgcacaa ggtcaaactg gtttggctgg aaacgccgtc
    40261 caatccactt ttacgcttgg tagacatcaa agtccttgcc gcaaaagcca aagcagccgg
    40321 tgcgctggtc ggtatcgaca acacttttgc cacgccgtat ctgcaacagc cgttggatat
    40381 gggttgcgat tttgtattcc attccgctac caaatatttg tgcggccatt ccgacgtgtt
    40441 gatgggcatc gtcgttgcca aaaccaaaga actggcgcag cctttgcacg atatgatggt
    40501 gcataccggc gcggttgccg gcccgctgga ctgctggctg gtgttgcgcg gcatcaaaac
    40561 actggctctg cgcatgaacg cccattgcca aaacgcactc gaaatcgcgc gccgtttgga
    40621 agcccatcct gccattgaaa aagtgttcca tcccggcctg ccgtctcacg aacattacga
    40681 actggcgaaa acacaaatgc ccaaaggcat cggcggcgtg gttacggttt atctcaaaaa
    40741 cgacacgcgt gaagcggcaa acagcgtgat taaaaacatg aaactggtca aaatggcttc
    40801 cagcctcggc ggtgtggaaa gtttggtcaa ccattgctat tcccagtccc acagcggcgt
    40861 accgcatgat gtgaaaatgg aaatgggcat caaagtcggt ctgctgcgtt tctccatcgg
    40921 catcgaagac gcggacgata tttggaacga tatttccgcc gcactcgata cgactttgta
    40981 aactgtaaaa atgccgtctg aaaccatggt ttcagacggc atttcaatta acccggccga
    41041 aaatcaacgc ttcaacatct ttgccgcctc aatagcgtaa taggtcaaaa tcccgtccgc
    41101 acccgcacgt ttgaatgcca gcaggctttc caaaaccact ttgccgccgt ccagccagcc
    41161 gttggcaatc gctgcctgca acatcgcgta ttctcccgaa acctgatagg cataagtcgg
    41221 tacgccgaac tcgtccttca cgcggcggac aacgtccaaa tacggtaaac cgggcttcac
    41281 catcaccata tccgcacctt cccgaatatc gagcgccact tcattcagcg cctcatcggt
    41341 atttgccgga tccatctggt aggttttctt gtccgccttg cccaaattgc ccgaactgcc
    41401 caccgcatcg cggaacggcc cgtaaaacgc cgaagcatat ttggcagaat acgccataat
    41461 ccgcgtatgg atatgcccgg catcctccaa cgcctcgcga atcgcaccga tacgcccgtc
    41521 catcatatcg gaaggggcaa ccacctgcgc gcccgcttca gcgtggcaca aagcctgctt
    41581 gaccaaaacc tctacggttt catcgttcat cacataaccg ttttcgtccg tcagcccgtc
    41641 ctgaccgtga accgtataag gatcgagcgc gacatccgtc ataatgccca gttcgggaaa
    41701 cctctcgcgc aaggcgcgga cagttgacgg cacgagtcct tcgggattgt acgcctcctg
    41761 cgcacgctcg gttttgtttg ccgtaaccac ggggaacagt gccaacatcg gaataccgag
    41821 ctttaccgcc tcttccgccg taaacagcag cctgtccaaa ctttgacgct tcacacccgg
    41881 catagaaggc acatcctcct cgcgcgccga cccctccaat acgaacaccg gataaatcaa
    41941 atcatcggcg gtcagcgtgt gttcgcgcat caggcggcgt gaaaaatcgt ccctgcgcat
    42001 acggcgcata cgcgaagccg gaacattgcg gtaaggaaac tgcataagcc ctccaatcat
    42061 cctgtcaaca attcaaacca tacggaagcc gccgcaactt gcagacagca tcgggcggca
    42121 atcaaaatcc gttttcaccg gaaagggttt cggcccgccg aaatgccgcg ccgaaggacg
    42181 gcgtcccgcc cattaaagaa caatagcaaa acaacagctt caggctgtca gccttcgata
    42241 ttcttacgcc acaataccaa aagtttcccg atatgctgaa caggttgcgc gtcaaccgcc
    42301 tcacataagg cagtgcagat ttcgatacgt tcggcacggt cgtcgccgaa tacgcgcact
    42361 ttaatcagct catgcgccgt caatgccgca tcggtttcct tgatgaccgc gtccgtcaga
    42421 ccctgctgac cgaccatcac aacaggatgg agatggtgcg cgcgcgcttt cagttccaaa
    42481 atttctttgg tgttcaattt ggtatccgtc attttcctac tgcttgaata aaaagaataa
    42541 tgcggcattg tacgcgattt ggcacgacat cggcaaaatt aacgtacaat acgcggtttc
    42601 cattccgacc cgcgaaccac tatggctgta cgttccaaat cctcaaaagc gtggctgcac
    42661 gaacacgtca acgaccacta cgtccatatg gcgcaaaaag acggctaccg cgcccgtgcc
    42721 gcatacaaac ttttggaaat caacgaaaaa gacaaattaa tcaaacccgg cacggtactt
    42781 gccgacttgg gcagcgcacc gggaagctgg tcgcaggttg ccgccaagct gacgggtact
    42841 tccggagcag ttttcgcctt ggacatcctg cctatggaag ccataggggg cgtctccttc
    42901 attcagggcg acttccgcga gaacgacgta ctggcacaat tcgaaacctt gttggacaac
    42961 cgcccgctcg accttgtaat ttgcgatatg gcgcccaata tgtcgggaaa cgccgtaagc
    43021 gatcaggcac gcagctttta tctgtgcgaa ctggctttag acttcgcctc gcaacacctg
    43081 aaaaccggcg gcagcttttt ggtcaaagtc tttcagggtg caggctatca ggaatacatg
    43141 gcagccatgc gcgaaatttt cggcacggtg cagacgcgca aacccgaagc ctcgcgcaat
    43201 cgctccagtg agatttattt attgggcaaa aataaacgct gacaatacag acggcgtgct
    43261 ttacaatcat ttccgtttta cacctcaatt atggagcctt gctaagtggg gaacaccttt
    43321 aaatcaatcc ttgtctgggt cgccttgggt atcggcctga tggctgcgtt caacgcttta
    43381 gacggtaaaa aagaagacaa cgggcaaatc gaatattctc agttcatcca acaggtcaac
    43441 aacggcgaag tatccggcgt caacatcgaa ggatccgtcg tcagcggcta cctgattaag
    43501 ggcgagcgca ccgacaaaag caccttcttc accaacgcgc ctttggacga caacctgatt
    43561 aaaacactgc tcgacaaaaa cgtccgtgta aaagtaacgc cggaagaaaa accgagcgcg
    43621 ctggctgccc tgttttacag cctgctgccc gtcctgctgc tgattggcgc gtggttctac
    43681 tttatgcgta tgcagacggg cggcggcgga aaaggcggcg cattctcatt cggcaaaagc
    43741 cgcgcccgcc tactggacaa agatgccaac aaagttacct ttgccgatgt cgccggctgc
    43801 gacgaagcca aagaagaagt gcaggaaatc gtcgattacc tcaaagcgcc gaaccgctat
    43861 caaagcctgg gcgggcgcgt gccgcgcggc atcctgctgg cgggcagccc gggtacgggt
    43921 aagacgcttt tggcgaaagc gattgcaggc gaagccggcg tgccgttctt cagcatttca
    43981 ggttccgact ttgtcgaaat gttcgtcggt gtcggtgcaa gccgcgtccg cgatatgttc
    44041 gagcaggcga agaaaaacgc cccctgcatc atctttatcg acgagattga cgcagtcggc
    44101 cgccaacgcg gcgcaggttt gggcggcggt aatgatgagc gcgagcaaac attaaaccaa
    44161 ttgttggttg aaatggacgg ttttgagagc aatcagactg taattgtgat tgcggcaacc
    44221 aaccgccccg acgtactcga tcctgcgctg caacgccccg gccgtttcga ccgccaagtg
    44281 gttgtccccc tgccggacat ccgggggcgc gaacagattt tgaacgtcca ctctaaaaaa
    44341 gtgcctttgg acaaatctgt ggatttattg tccctcgcgc gcggcacgcc gggtttttcc
    44401 ggcgcggatt tggcgaactt ggtcaacgaa gccgccctgt ttgccggccg ccgcaataaa
    44461 gtcaaagtcg atcagagcga ttttgaagac gccaaagaca aaatctatat gggtccggaa
    44521 cgccgcagta tggtgatgca cgaagacgaa aaacgtgcga cggcgtatca cgaatccgga
    44581 cacgcgattg ttgccgaaag cctgcccttt accgaccccg tccacaaagt aaccattatg
    44641 ccgcgcggac gtgcgctggg tctgacttgg cagcttccgg agcgcgaccg catcagtatg
    44701 tataaagatc agatgttgag ccagctctcc atcctgttcg gcggacggat tgccgaagac
    44761 atcttcgtcg gacgcatctc caccggcgca tcaaacgact ttgaacgcgc aacccaaatg
    44821 gcgcgcgaaa tggtaacgcg ctacggcatg agcgacaaaa tgggcgtgat ggtttatgcg
    44881 gaaaacgaag gcgaagtctt cttgggacgc agcgtaaccc gttcgcaaaa catttccgag
    44941 aaaacccagc aggacatcga tgcggaaatc cgccggattt tggacgagca gtatcaggtt
    45001 gcctacaaaa tcctcgatga aaaccgcgac aagatggaaa cgatgtgcaa agccctgatg
    45061 gaatgggaaa ccatcgaccg cgatcaggta ctggaaatca tggcgggcaa acaacccagc
    45121 ccgcccaaag attacagcca caacctgcgc gagaatgcgg acgcggcgga agataacgcg
    45181 ccgcacgctc cgactcggga agaaaccgaa gcacctgccc cggcagacac cgcttcgaca
    45241 gagtccgagc agcagcctga aaacaaggct taacttcccg aacaaacggc agcccgcaag
    45301 ctgccgtttt cccatcctgt tttcagacgg cattttccgc tcaatgccgt ctgaaacgcc
    45361 aaagccgtat cggtttttgc gaatctgcga tatggcggtt taaattgaaa tcagaacggg
    45421 gcaaggctgt accggtttaa aattaaaccg ctataatacc gcccttcgaa tacacgcccg
    45481 cattggtgat gcccgtgaaa aaagttgaaa aaaacatctt ggtgctgcac ggcgcggaca
    45541 aaatgttcga gctggtcgat aaggttgagg attacccgaa ctttctgccg tggtacagca
    45601 aaaccgaggt catcggacgc aacggcaacg aactgaaggc gcggctgttt atggactata
    45661 tgcacgttcg ccaatcattc gccacgcaca accgcaacat tccgggcagg gaaatccgta
    45721 tggaactgct cgaaggtccg ttcaaaacct tacgtggaac gtggaaattt atcgatttgg
    45781 gcgacgatat gtgcaaaatc gaattcaatt tggaatacga tttttccaat gccgttttgt
    45841 ccgccttaat ttcccccgtc ttcaaccacc tttccgccac gctggtcgaa gcgttcgtca
    45901 aagaggcaga ccgccgttat gcttgaaatt gagattgtgt acgggctgcc cgaccgacag
    45961 gttttgaaaa ccatgcagct tgccgaggga acaaccgtcc gcgccgccgc actgcaaagc
    46021 ggtttggacg gcatatttga agatttaaac ctgcattccg cgcctttggg cattttcggc
    46081 aaagccgtca aagacgacac gccgctgcgc gacggcgacc gcatcgaagt gtaccgcccg
    46141 ctgttgatcg accccaaaga agcgcgccgc aaacgcgttc aaaatcaaga agaataacca
    46201 tgccgtctga agccttcaga cggcattcgg cagaaacacg gaaaatatca tgtcaaacac
    46261 aatcaaaatg gttgtcggct tgggcaaccc gggcaaagaa tacgaacaga cccgccataa
    46321 tgcaggcttt tggttcctcg acgaactggc ttggaagtgg aaggcttcgt ttaaagaaga
    46381 aaaaaaattc ttcggcgaag tcgcccgtgc taccctgccc gacggcgatg tctggctgct
    46441 caagccgacc acgttcatga accgttccgg acaggcagtt gccgcccttg cgcagtttta
    46501 taaaatcaaa cccgaagaaa tcctcgtcgt ccacgacgaa ctcgacattc cctgcggacg
    46561 gatcaaattc aaactcggcg gcggcaacgg tggacacaac ggcttgaaag acattcaggc
    46621 acggctcggc acggccgact attaccgcct gcgcctcggt atcgaccacc cgggcgaccg
    46681 caacctcgtc gtcggctatg tcctgaacaa acccagtgcg gaacaccgcc aacagattga
    46741 tgatgccatc gccaaatccc tgcaggccat acccgacatc atttccggca aatgggaaga
    46801 agcaacacgt ttcctgcaca gcaaatgacc caatgccgtc tgaagccctt tcagacggca
    46861 ttcccgccat cagcgagaac ccgcactatg tccgaactca accggctcat ccaaaccgaa
    46921 tccgttgccg tcattgaaga aactttaaat tttctgctct acgaatgcag catagacgat
    46981 gccccctccg ccgaagaaat tgccgtttgg cgcgatatgc tggccgcacg cggcggaaaa
    47041 ttcctgcgcc tgtccaaaat ctgccaaacg tggctggacg aggaggcggc atgaagctgc
    47101 cgcgcaaccg cttcagcctg ctttccgcat tgtggtttgc cggcggcatc tattcgctgc
    47161 tcttcaaagc tgccgacacc gcgccgccgc cgtttccgca tttcgacaaa gcagcacacc
    47221 ttgccctgtt tttcgcacaa atctagcttt tgaccaaagc attcaaaacc ggaaaacttc
    47281 ccatccccta ccgcagcctg atggtctttg ccctctgttt cgccctcttc agcgaatgcg
    47341 cgcaggcatg gtttaccgca acgagaaccg gcagtttggg cgatgttctt gccgatatgg
    47401 caggtacggt tctcgcactc tttgccgccc gcgccgccga ccgcccggac tgaatcggtt
    47461 tatttccccc aaacaggatg cacctcttcc cgatacatcc tatttttccc cttacatatc
    47521 aatcccatct cataaacaaa accgacaaat cgtttacaat atatttacac tacatcagat
    47581 tacaaatata ctcgaaccag ttgcagaatc ggcggttcca caaaccgttt cggattgcac
    47641 gacacaaaag aaaaaccgat tttattgcct taaaggagta ttcatgaacc tgcatgcaaa
    47701 ggacaaaacc cagcatcccg aaaacgtcga gctgctcagt gcgcagaagc cgattaccga
    47761 ctttaagggc ctgctgacca ccattatttc cgccgtcgtc tgtttcggca tttaccacat
    47821 cctgccttac agccccgatg ccaataaagg tatcgcgttg ctgattttcg ttgccgcact
    47881 ttggtttacc gaagccgtcc acattaccgt taccgcgctg atgatcccta tcctcgccgt
    47941 cgtactcggt ttccccgaca tggacatcaa aaaggcgatg gctgattttt ccaacccgat
    48001 tatctacatt tttttcggcg gtttcgcact cgccaccgcc ctgcatatgc agcgtctcga
    48061 ccgcaaaatc gccgtcagcc tgctgcggct gtcgcgcggc agtatgaagg ccgccgtgct
    48121 gatgttgttc ctcgttaccg cctttctgtc catgtggatc agcaacaccg ccaccgccgc
    48181 gatgatgctg cctctggcaa tgggtatgct gagccacctc gaccgggaaa aagaacgcaa
    48241 aacctacgtc ttcgtcctgc tcggcatcgc ctattgcgcc agcatcggcg gcttgggcac
    48301 gctcgtcggc tcgccgccca acctgattgc cgccaaagcc ctaaatctgg acttcgtcgg
    48361 ctggatgaag ctcggcctgc cgatgatgct gttgattctg cccttgatgc tgctctccct
    48421 gtacgtcatc ctcaaaccta atttgaacga acgcgtggaa atcaaagccg aatccatccc
    48481 ttggacgctg caccgcgtga tcgcgctgtt gattttcctt gccacagccg ccgcgtggat
    48541 attcagctcc aaaatcaaaa ccgccttcgg catttccaat cccgacaccg ttatcgccct
    48601 gagtgccgcc gtcgccgtcg tcgtcttcgg cgtggcgcaa tggaaggaag tcgcccgcaa
    48661 taccgactgg ggcgtgttga tgctcttcgg cggcggcatc agcctgagca cgctgttgaa
    48721 aacatccggc gcgtccgaag ccttgggaca gcaggttgcc gccacctttt ccagcgcgcc
    48781 cgcatttttg gtgatactca tcgtcgccgc cttcattatt tttctgaccg agttcaccag
    48841 caacaccgcc tccgccgcat tgcttgtacc gattttctcc ggcatcgcta tgcagatggg
    48901 gctgcccgaa caagtcttgg tattcgtcat cggcatcggc gcatcttgtg ccttcatgct
    48961 gccggttgcc acaccgccta acgcgattgt gttcggcaca ggcttaatca agcaacgcga
    49021 aatgatgaat gtcggcatac tgctgaacat cctctgcgta gtattggttg ctctgtgggc
    49081 ttatgctgta ctgatgtaaa ccatcgacct aaacaacaag accgtctgaa agaatatttt
    49141 tcagacggcc ttgaagtttg tcgttcagac acaatttgtc gaatcattca aaaccggatt
    49201 ctaacgaaag gaaacccatg attatcctgc acaccaacaa aggcgacatc aaaatcgaac
    49261 tcgatttcga caaagccccc gttaccgcca aaaacttcga gcaatacgtc aaagacggct
    49321 tctacgacgg cgtaatcttc caccgcgtca tcaaaggctt catgattcaa ggcggcggca
    49381 tggatgaaaa catgaacgaa aaagaaaccc gcgatccgat tcaaaacgaa gcgtccaacg
    49441 gcctgcccaa cgataaatac accatcgcca tggcacgcac ttccgacccc cattccgcca
    49501 gcgcgcaatt cttcatcaac actgccgaca acgctttcct gaacttccgt tctaaagagc
    49561 tgtacggcaa aaccgtcgtc caagactggg gctatgcagt attcggtaaa gtcgttgacg
    49621 gttttgacgt tgtcgatgcc atcgaaggcg tatccaccaa acgccacggc taccatgacg
    49681 acgtaccgag cgaacctgtc gtcatcatta aagccgaagc ggtataaacc ggcaatccga
    49741 aagcagcctg cacaaaggct gctttttttg tttcaggcgg cataaaatcc aagcacatat
    49801 gccgtctgaa cacctcccgc aagccttcgg acaatgttgc aacgtgttaa aatagcctcc
    49861 tttcctgaaa ccacaacaag gaaaccatca tggcaaatat tgcccgcgac atcttcaaag
    49921 cctacgacat ccgaggcatt gtcggcaaaa ccctgactga cgatgccgcc tatctcatcg
    49981 gcagggccat cgccgccaaa gccgccgaaa aaggcatgac ccgcatcgcg ctcggacgcg
    50041 acggacgctt gagcggtccc gaactgatgg agcacatcca acgcggcttt accgacagcg
    50101 gcatcggcgt actcaacgtc ggcatggtcg ccactcctat gctctatttt gccgccatca
    50161 acgaatgcgg cggcagcggc gtgatgatta ccggcagcca caatccgcct gattacaacg
    50221 gtttcaaaat gatgctcggc ggcgacacgc tcgcaggcga agccattcaa gaacttttag
    50281 ctattgttga gaaagatggt tttgttgccg ccgacaaaca aggcaacgta accgaaaaag
    50341 acatctccgg cgaataccac aaccacatcg tcggccacat caaactcaaa cgccccatga
    50401 aaattgccat cgacgcgggc aacggcgtgg gcggcgcgtt tgccggcaaa ctctataaag
    50461 gcttgggcaa cgaagtaacc gagcttttct gcgacgtgga cggcactttc cccaaccacc
    50521 atcccgaccc atccaaaccg aaaaacctgc aagatttgat tgccgcgctg aaaaacggtg
    50581 atgccgaaat cggcctggcg tttgacggcg atgccgaccg cttgggtgtg gtcaccaaag
    50641 acggcaacat tatttatccc gaccgccaac tgatgctgtt cgcccaagac gttttgaacc
    50701 gcaatcccgg cgcgaaagtc attttcgacg tgaagtccac ccgcctgctt gcgccttgga
    50761 ttaaagaaca cggcggcgaa gccataatgg aaaaaaccgg ccacagcttt atcaaatccg
    50821 ccatgaaaaa aaccggcgcg ctggttgccg gcgaaatgag cggacacatc ttcttcaaag
    50881 aacgctggtt cggcttcgac gacggtctgt acgccggcgc acgcctcttg gaaatcctgt
    50941 ctgcctccga taatccgtcc gaagtgttaa acaacctgcc gcaaagcatt tccacgcccg
    51001 aactcaacat cgccctgccc gaaggcagca acggccatca ggttatcgac gaactcgccg
    51061 ccaaagccga atttgaaggc gcaaccgaaa tcatcaccat cgacggtctg cgcgttgaat
    51121 ttcccgacgg cttcggtctg atgcgtgctt ccaataccac gccgattctg gtgttgcgtt
    51181 ttgaggcgga tacgcaagca gccatcgagc ggattcaaaa ccaattcaaa gccgtcatcg
    51241 aaagcaatcc gaatctaatc tggcctctgt aaacacagga aaaatgctta atcaaatctt
    51301 ggtagattgg atttggctta aaaatctccg cgtccgttcg tgtttggggt ggtcgaacaa
    51361 ctctttcgtg ctgccctgct ctacgataac gccgccgtcc atcacgacaa cggtcgtggc
    51421 aacttccagc gcgaacttga tttcgtgggt aacgacgacc atcgtccaac cttcccgcgc
    51481 caattccttc atggcgttca acacgtcttg caccaactcg gggtcaagcg cggaagtggg
    51541 ttcgtcaaac aacatcagct cgggctgaat cgccaatgct cgggcaatgc cgacgcgctg
    51601 ctgctgaccg ccggaaagct ggtagggata caaatccact ttgtcgccca agccgacttt
    51661 ttccagcagt ttcagagcct cttcgcgcgc ttgggcggca ggcttgccct gtacggcaac
    51721 cggtccttcc atcacgtttt ccaaggcggt tttgtgcgga aagaggttgt attgttgaaa
    51781 caccatgcct gatttgcggc gcagtgccaa aatatcgtgt ttgcttggtt ttttagaaaa
    51841 atcgattttc agcggtcgct cgttgtcgaa ctcgatttgt ccgtcttcgg gcatttccaa
    51901 cgcgtttagg catcgcagaa acgtcgtttt gcctgagccg gaaggcccga ggatgacgac
    51961 cacctgccct ttgcacacat ccaaattgat gccgcgcaaa atggtatttt tgccgaaggt
    52021 cttatggata ttgcggattt taatcatgac aactccttat ttggcgacgt agcggtcgaa
    52081 acgtttttcc aaacgcgcct gaatcaggaa cagcacttta caaaaacacc agtaaaccaa
    52141 agcggcttcg atatagacgg gcaaaaagtc ataagtgcgg tttgccgttt cctgcgcgac
    52201 gcggaataat tccgttaccg tcacgactgc cgcgagcgag gtgtttttaa acaaaccgat
    52261 aaactcgttg ctcaaaggcg gcacggcaac gcggaatgcc tgcggcgcaa cgatgcggcg
    52321 gaacgtctgc atataggtca tgccgatgga gaaacctgct tcccattggc ctttcggtac
    52381 ggacaaaatt gccgcgcgta tggtttcgga agcatatgcg ccgacattga gcgaaaagcc
    52441 gatgatggcg gcaggaatcg ggtcgatata gatgccgacg gaaggcagcc cgtaaaacac
    52501 aatcacaagc tgaaccaaca gcggcgtacc gcgaatgacg gaaatataaa attccaccaa
    52561 tttcagcagg attttccgca cgatgccgcc ggcgggcata atccgcacca aagccacggc
    52621 taccgcaatc atcataccga taacgaaaga agctgccgcc aaaggcagag agaccgcgaa
    52681 gccggctttg accataggca aaaacgcgct gacaatcata tcggcgcgtg tttccgtcat
    52741 aaacggcagc gaagcaagga aattattgaa cactgatgtc ttttccgaag aattgttcgc
    52801 ccagtttttt cagcgtaccg tcggctttca gctcgttgat tgccgtactg aatttcgcca
    52861 cggcttcgtc attgcccttg ttgacaatca ggccggaacc gactttttca tcggcaggtg
    52921 cggaccaaac gattttcacg cccgcattcg ggtttttctt cagatagtcc aaaaccgcca
    52981 attcgtcgtt cagggttgca tcggcacgtt tttgttcaat cagggtcagc gattgcgcca
    53041 aaccgtccac tgccaccaaa tctgcgcctg cagctttggc tttttcgccg tagttgctgg
    53101 tcagggattg tgcggttttc acgcctttga tgtcgtcgat agatttgatg ttgctgtcgt
    53161 tacgggcaac caatacggca ccgctccagc tgtaaggatc ggatttgtcg aatgtcgctt
    53221 ggcgttcggg gctggtcaga ccgacttggt ttgccaccac gtcgaaacgc cccgccttca
    53281 aacccgccat catcgaatcc cattgcgttt ctttaaactc gactttcacg cccagttttt
    53341 ccgccacggc gcgggttact tccacatcgt aaccggtcag tttgccgtcc ttgtcgtggt
    53401 aggtaaacgg tgcgtaagtg ccttccgtgc cgacggtaac cgtgccttta ttgttgatgc
    53461 gctcgattaa agaaccggaa actgccgatt gtgcaggcgc ggaagatgct ccgctgccgc
    53521 tttccgaacc gccgcaggcc gccaaaacca atgcggcaat gccgccgagt acgaattttt
    53581 tcaacatcat tctctcctgt aaaaagtgtg caagtgcggc attctaagtg aaattgcggc
    53641 aaacacaaag aatacttttt ttatatggtt attccaaatc ggaatggaaa agatatgccg
    53701 aagccgccgg aagcggaaat aaagggggcg ggaaaggcgg cgggcggcac gaaaccgaaa
    53761 agcggcatca ggtatgcaaa cggcggattc acgcacgccg ttccggcaaa aatccgcctg
    53821 ccgattcggt gctataatcc atccgaaaca gatgacacca aaaaggaaca tctcatgaac
    53881 acgccccaat ccgccatcat tcccgaccac gcccaagccg gcatctttat cgaagccgac
    53941 ttcgccgcca accgcctcaa cgatattaaa gccgcctgcc gcgcttcgct cgacgcgtta
    54001 tccgccttga aaacccgctt tccggacgat attttgggtc tgaccatcgc cttcggcagc
    54061 aaagcctggg caacattcgg acatacggac gaaggcagtg aaatcaaacc cttctccgaa
    54121 atgggcaacg ggcttgcgcc gtccacgcag cacgatatgt ccatccacat ccaatctttc
    54181 cgccaaaacg ccgcctacgc gcttgcccaa tccgttttgg gcgcgttcgg cgacagcata
    54241 tgcgtcgcgt ccgaagaaca cggtttgcgc ctgtatcagg atcgcgggct ggacggtttc
    54301 gtcgacggca cggaaaaccc gcagggcgat gaaaatgtcc gcgaagtcgc catcatcccc
    54361 gaaggaaaac ccgatgcggg cggcagctat gtcctgctgc aaaaatacct gcacgatttg
    54421 aaaaaatggg atgccgtccc cgtcgccgaa caggaggcct cggtcggacg cggcaaggaa
    54481 accgacgacg aattcggcca cgatgtccgc ctgcccgatt cgcacctcgg ccgcgtcaac
    54541 ctgaaagaaa acggagtcgg cctgaaaatc gtccgccgca gcctgccctt cggcaaaatc
    54601 agcggcgaac acggtttgat gtttaccgca tactgccgca cgctgcacaa tatcgaagcg
    54661 caattattga gtatgttcgg cgacacggat ggcaaaaccg acctgctgct ccggcacctc
    54721 tccgccgccg tttcgggcgg atactactac gcgccgtctg tcgaacgcct gcaaaacctc
    54781 taagccagac gatgccgtct gaaaccattt ttcagacggc atttt
//
LOCUS       DAWKOG010000002        40286 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.002, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000002 DAWKOG010000000
VERSION     DAWKOG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40286
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.002"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(396..3002)
                     /gene="acnD"
                     /locus_tag="WJK66_000056"
     CDS             complement(396..3002)
                     /gene="acnD"
                     /locus_tag="WJK66_000056"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HEZ7577837.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNREIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDSLGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELTGKRKAGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     IYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATADLASKGLAKPYE
                     EPSDGQMPDGAVIIAAITSCTNTSNPRNVVAAALLARNANCFGLKRKPWVKTSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLS
                     GNRNFDGRVHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGKEIRLKDIWPA
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILAVSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVRNEDGSVRQGSLARVEPEGQTMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLQFKPDTNRH
                     TLQLDGTETYDVVGERTPRCDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
     gene            complement(3082..3870)
                     /locus_tag="WJK66_000057"
     CDS             complement(3082..3870)
                     /locus_tag="WJK66_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEZ7577838.1"
                     /translation="MEDLYIILALGLVAMIAGFIDAIAGGGGLITLPALLLAGIPPVS
                     AIATNKLQAAAATFSATVSFARKGLIDWKKGLPIAAASFAGGVVGALSVSLVSKDILL
                     AVVPVLLIFVALYFVFSPKLDGSKEGKARMSFFLFGLTVAPLLGFYDGVFGPGVGSFF
                     LIAFIVLLGCKLLNAMSYTKLANVACNLGSLSVFLLHGSIIFPIAATMAVGAFVGANL
                     GARFAVRFGSKLIKPLLIVISISMAVKLLTDERNPLYQMIVSMF"
     gene            complement(4031..5185)
                     /gene="prpC"
                     /locus_tag="WJK66_000058"
     CDS             complement(4031..5185)
                     /gene="prpC"
                     /locus_tag="WJK66_000058"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEZ7577839.1"
                     /translation="MTETTQTPTFKPKKSVALSGVAAGNTALCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETIGGHFLQLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            complement(5271..6146)
                     /gene="prpB"
                     /locus_tag="WJK66_000059"
     CDS             complement(5271..6146)
                     /gene="prpB"
                     /locus_tag="WJK66_000059"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEZ7577840.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            complement(6772..8082)
                     /locus_tag="WJK66_000060"
     CDS             complement(6772..8082)
                     /locus_tag="WJK66_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEZ7577841.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            complement(8221..9399)
                     /gene="ftsZ"
                     /locus_tag="WJK66_000061"
     CDS             complement(8221..9399)
                     /gene="ftsZ"
                     /locus_tag="WJK66_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEZ7577842.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(9518..10762)
                     /gene="ftsA"
                     /locus_tag="WJK66_000062"
     CDS             complement(9518..10762)
                     /gene="ftsA"
                     /locus_tag="WJK66_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEZ7577843.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQTPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREGGGGLLARLKRWIENSF"
     gene            complement(10848..11576)
                     /locus_tag="WJK66_000063"
     CDS             complement(10848..11576)
                     /locus_tag="WJK66_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEZ7577844.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYASDGLPEKESEE"
     gene            complement(11566..12480)
                     /locus_tag="WJK66_000064"
     CDS             complement(11566..12480)
                     /locus_tag="WJK66_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEZ7577845.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKGKGRLKS
                     VYEELKHFQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRNDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTGH
                     SLVPKSAAVMGVGFADLCIEILKTAHVG"
     gene            complement(12593..14002)
                     /locus_tag="WJK66_000065"
     CDS             complement(12593..14002)
                     /locus_tag="WJK66_000065"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEZ7577846.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLSAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLELSKQI"
     gene            complement(14156..15223)
                     /gene="murG"
                     /locus_tag="WJK66_000066"
     CDS             complement(14156..15223)
                     /gene="murG"
                     /locus_tag="WJK66_000066"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEZ7577847.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPFTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDNARPQMYHQ
                     SGRGKLGSLQADYDALGVQAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(15227..16552)
                     /locus_tag="WJK66_000067"
     CDS             complement(15227..16552)
                     /locus_tag="WJK66_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HEZ7577848.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(16678..18015)
                     /locus_tag="WJK66_000068"
     CDS             complement(16678..18015)
                     /locus_tag="WJK66_000068"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEZ7577849.1"
                     /translation="MTFQNKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAELQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKDKIFRGDGVQV
                     LNADDTFCCAMKRAGREVKWFSLEHEADFWLERETGRLKQGNEDLIATQDIPLQGLHN
                     AANVMAAVALCEAVGLPRNELLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALAGKAKGVFLIGVDAPQIRHDLDGCGLN
                     MTDCATLEEAVQKAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIGAFKAL"
     gene            complement(18099..18371)
                     /locus_tag="WJK66_000069"
     CDS             complement(18099..18371)
                     /locus_tag="WJK66_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ7577850.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAAQGNAAAQ
                     NNLGVMYAERRGVRQDRALAQEWLGKACQNGYQDSCDNDQRLKAGY"
     gene            complement(18486..19568)
                     /locus_tag="WJK66_000070"
     CDS             complement(18486..19568)
                     /locus_tag="WJK66_000070"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEZ7577851.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLATFPVVLVAAGLAIFAYA
                     SGHSQFAQYLQLPYVAGANEVVIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
     gene            complement(19662..19838)
                     /locus_tag="WJK66_000071"
     CDS             complement(19662..19838)
                     /locus_tag="WJK66_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7577852.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMQTQAYLYEDLCLDLGGGKNP
                     GSYPICVIGKVSAR"
     gene            complement(19841..21199)
                     /locus_tag="WJK66_000072"
     CDS             complement(19841..21199)
                     /locus_tag="WJK66_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEZ7577853.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALA
                     GGRFDAHDFVEDVLAAGAAAVVVSREDCVAMDGALKVDDTLTALQMLAKAWRENVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDNAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     MALIPQEDANMAVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGNECAAV
                     VLPVPGRHNVHNAAAAAALSLAAGLSLNDVAEGLKGFSNIKGRLNVKSGIKGATLIDD
                     TYNANPDSMKAAVDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(21324..22970)
                     /locus_tag="WJK66_000073"
     CDS             complement(21324..22970)
                     /locus_tag="WJK66_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HEZ7577854.1"
                     /translation="MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWRYCVPGGVFADVKNYKRRSKIWLTI
                     LLTLILSCAVMDKIASDKDLREPDAGLLLNIFDLYYDLASAPAQYAAKRAHILEAAKK
                     ASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSQTKGLLINGYQSTAH
                     ATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPW
                     FTQRGDYGKSAGLSDRLLLPAFKRVLIGNAGTKPRLIVMHLMGSHSDFCTRLDKDARR
                     FQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLT
                     HGAWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFW
                     GNVPDVQGEGNNLAFIDGLPDDPAPWYAGKGKSTKNTSKK"
     gene            complement(22993..24471)
                     /locus_tag="WJK66_000074"
     CDS             complement(22993..24471)
                     /locus_tag="WJK66_000074"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEZ7577855.1"
                     /translation="MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKFWGVTGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATVAAMEVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINVDDEYGAELVGRLKKDCPDLAVYSYGFSEHADIRIIDF
                     TASSDGIAAVFQTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDKVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(24496..26241)
                     /locus_tag="WJK66_000075"
     CDS             complement(24496..26241)
                     /locus_tag="WJK66_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEZ7577856.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGR
                     YADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILGI
                     SPTKPLTAAAVKTPS"
     gene            complement(26302..26565)
                     /gene="ftsL"
                     /locus_tag="WJK66_000076"
     CDS             complement(26302..26565)
                     /gene="ftsL"
                     /locus_tag="WJK66_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEZ7577857.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVEHQR"
     gene            complement(26562..27548)
                     /gene="rsmH"
                     /locus_tag="WJK66_000077"
     CDS             complement(26562..27548)
                     /gene="rsmH"
                     /locus_tag="WJK66_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEZ7577858.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAIRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PELPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGPIPEQSQRKTSEWQ"
     gene            complement(27545..28000)
                     /gene="mraZ"
                     /locus_tag="WJK66_000078"
     CDS             complement(27545..28000)
                     /gene="mraZ"
                     /locus_tag="WJK66_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEZ7577859.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLVPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLGQTDLQL"
     gene            28279..29496
                     /locus_tag="WJK66_000079"
     CDS             28279..29496
                     /locus_tag="WJK66_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ7577860.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTLIMPLPLPSPEARQSSLKLQTLI
                     AEKIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLAR
                     QLQELLSQTAGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQAR
                     APEASQKVVHLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTY
                     SARPLHDLQLSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLEHGCMIFIDYGFDA
                     AQYYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLIGYL
                     PQSHFLLNLGITELLAQTGKTNSAAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGID
                     WAGFRFGDICHKL"
     gene            29628..29704
                     /locus_tag="WJK66_000080"
     tRNA            29628..29704
                     /locus_tag="WJK66_000080"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29662..29664,aa:Met,seq:cat)
     gene            29712..29787
                     /locus_tag="WJK66_000081"
     tRNA            29712..29787
                     /locus_tag="WJK66_000081"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29745..29747,aa:Ala,seq:ggc)
     gene            complement(29919..30023)
                     /locus_tag="WJK66_000082"
                     /pseudo
     CDS             complement(29919..30023)
                     /locus_tag="WJK66_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(30079..30900)
                     /locus_tag="WJK66_000083"
     CDS             complement(30079..30900)
                     /locus_tag="WJK66_000083"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEZ7577861.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIDFHSNHK
                     VFEITIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIVDVDALRPIDALMIGVAQVFA
                     LVPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFIAAFVSGLVAVKALLKFVSKKNYIPFAYYRIVFGIVIIILWLSGWISWE"
     gene            complement(30933..31577)
                     /locus_tag="WJK66_000084"
     CDS             complement(30933..31577)
                     /locus_tag="WJK66_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ7577862.1"
                     /translation="MKLKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQEQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVKLSGL
                     KYQANPAVFKAVYEQKIRLENRSVAEKWALSQKGFDGKKLMRAYDSPAAAAAASKMQQ
                     LTEQYRIDSTPTVVVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(31580..32425)
                     /gene="ftsN"
                     /locus_tag="WJK66_000085"
     CDS             complement(31580..32425)
                     /gene="ftsN"
                     /locus_tag="WJK66_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ7577863.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPA
                     SSKQPAETEILKPKNQPKEDIQPEPADQNALYEPDAATEAEQSDAEKAADKADEVEEK
                     AGEPEREEPDGQAVRKKVLTEEREQTVREKAQKKDAETVKKQAVKPSKETEKKASKEE
                     KKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTPDKAEATHYLQMGAYADRR
                     SAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHEVASLIR
                     SIESK"
     gene            complement(32531..34027)
                     /locus_tag="WJK66_000086"
     CDS             complement(32531..34027)
                     /locus_tag="WJK66_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEZ7577864.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECQVPQMPFEHGGQPDLCDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARDKQYARQGKVNAALSVSELDSQACIQKEAQEAL
                     GGLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(34041..34361)
                     /locus_tag="WJK66_000087"
     CDS             complement(34041..34361)
                     /locus_tag="WJK66_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEZ7577865.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            34844..36370
                     /gene="putP"
                     /locus_tag="WJK66_000088"
     CDS             34844..36370
                     /gene="putP"
                     /locus_tag="WJK66_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HEZ7577866.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKAYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWI
                     KKPAQAAGESGLLTMYEIVPGFIVCLIIAVLVSLFNKEPSREIQERFEKADADYRAAR
                     "
     gene            complement(36460..36627)
                     /locus_tag="WJK66_000089"
     CDS             complement(36460..36627)
                     /locus_tag="WJK66_000089"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7577867.1"
                     /translation="MKHFLDSPKFFGGQAASVRPEYGNIVINPQMKYKASNAGRVGAS
                     VSLKMKRVKHK"
     gene            36619..40224
                     /gene="putA"
                     /locus_tag="WJK66_000090"
     CDS             36619..40224
                     /gene="putA"
                     /locus_tag="WJK66_000090"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEZ7577868.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDKQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDTLIRSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADKLDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRIPEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWVHG
                     GSLLQAFCALTELAASGVQAVVEPDSPLASYTADLEGLLLVNGKPETADISHVAALSP
                     LDSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         9520 a        11417 c         9989 g         9360 t
ORIGIN      
        1 cgccttgtcc cgatttttgt taatcctcta taccatgtca attcagcctt tatttcgcgg
       61 gcaaagccca cgctacaccc actttccaga agtacattag gctgccactc cgacaaagcc
      121 gtttgattcc gcaaactgtc ggggttcgcc acaatctacg gctactgtgt tgtgatacgg
      181 cagattatta aaccccgtca ttcccgcgca ggcgggaatc caaatttgtc cgcacagaaa
      241 ctcatcggat aaataaggtt tcctcaattc cactttctgg attcccgcct gcgcgggaat
      301 gacgatttac agtagatgcc tgccatatcg agaattacgt gaatgagcaa aatgttgaac
      361 ccgacccacg ctacgtcttg cttttcagac gacctctaag ccgcgttccc ttccaaaaaa
      421 tcctgtgcaa accgttgcaa cacgccgccg gcttcatata ccaatacttc ttctgcagta
      481 tcgaggcggc aggtaacggg aacttcgacg gtttctccgt ttttacggtg aatcacgagg
      541 gtcaggtcgc agcgcggtgt gcgttcgccg accacgtcgt aggtttccgt accgtccagt
      601 tgcagggtat ggcggttggt gtcgggtttg aactgcagcg gcaacacgcc catgccgata
      661 aggttggtgc ggtggatacg ctcgaagcct tcggcaacaa tcgcttctac gccggcgagg
      721 cgtacgcctt ttgcagccca gtcgcggctt gagccttgac catagtccgc accggcaatg
      781 atgatgagcg gctgtttgcg gttcatatag gtttcgatgg cttcccacat gcgcatggtt
      841 tggccttcgg gttcgacgcg tgccagcgaa ccttggcgta cgctgccatc ttcgtttctc
      901 accatttcgt taaacagttt cggattggcg aaggtagcgc gttgggcggt caagtggtcg
      961 ccgcggtggg ttgcgtaaga gttgaagtct tcttcaggca aacccatttt cgccaaatac
     1021 tcgcctgcgg cactgacggc caaaatcgca ttggacggcg agaggtggtc ggtggtgatg
     1081 ttgtcgggca aaatcgccag cggacgcata cctcttaatg tgcgttcccc tgccagtgcg
     1141 ccttcccagt aaggtgggcg gcggatatag gtggacatcg gacgccagtc gtacagcgga
     1201 ctaggtgctt tttgcgctgt gccggtgtcg aacatcggta catacacatc gcggaactgc
     1261 tgcggtttca catattcggc aacgacggca tcgatttctt catcggcagg ccaaatgtct
     1321 ttcaggcgga tttccttgcc gtctgcaacg ccgagtacgt cgttttcaat atcgaaacgg
     1381 atgctgcctg ccagcgcgta ggcaacgacc aacggaggcg aagcgaggaa agcctgtttc
     1441 gcatacggat ggacacggcc gtcgaagttg cggttgcctg ataatacggc ggtggcgtac
     1501 aaatcgcggt cgatgatttc tttctggatt ttcggatcca gcgcgccact catgccgttg
     1561 caggtggtgc aggcgaaggc gacgataccg aagccgagtt tttccatttc gggcagcagg
     1621 cctgcttctt tcaaataaat ttccgccact ttcgaaccgg gggcaaacga ggttttgacc
     1681 cacggtttgc gtttcagccc gaagcagttg gcgttgcgcg ccaagagcgc ggcggcaaca
     1741 acgttgcgcg ggttggaagt gttggtgcaa ctggtaatcg cggcgatgat gaccgcgccg
     1801 tcgggcattt ggccgtctga aggctcttcg taaggtttag ccaagccttt gctggccaaa
     1861 tcggcggtcg caaaacgcgc gtgcgggttg ctcgggcctg ccatattgcg cgttacgctg
     1921 ctcaaatcaa atttcaaaac gcgcggataa acggcggttt tcaaggcatc tgcccataag
     1981 cctgcggttt tggcgtagat ttctaccaat ttcacttgcg cgtcgtcgcg accggtcagt
     2041 ttcaaataat caatggtttg ctcatcaata gcgaacatcg cggcagtcgc gccgaactca
     2101 ggggtcatgt tggaaatggt ggcacggtcg ccgatggaca ggcttcttgc accttcgccg
     2161 aagaattcga caaatgcgcc aaccacgcgc tctttacgca agaattcggt cagtgccaac
     2221 acaatatccg tcgccgtaat gcccgccttc cgtttgccgg tcagctcaac gccgacaata
     2281 tcaggcagac gcatcatgga tgcacggccc aacattacag tttcggcttc caaaccgccc
     2341 acgcctacag aaatcacgcc caatgaatcg acgtgcggcg tatgtgaatc tgtaccgaca
     2401 caggtatcgg ggaaagccac gccgtttttg acttggacga cgggcgacat tttttctaga
     2461 ttgatttggt gcatgatgcc gttgcccgcc ggaatcacgt ccacattttc aaaagcggtt
     2521 tttgtccagt tgatgaagtg gaaacggtct tcgttacgtc tgtcttcgat ttcgcggttt
     2581 ttgcggaacg catcggggtc gtagccgccg cattcaacgg ccagagagtg gtcgacgatg
     2641 agctgggttt gcaccaccgg attcactttg gcaggatcgc cgcctttttc ggcaatcgca
     2701 tcgcgcagac ctgccaaatc caccaacgcg gtctgcccca gaatatcgtg gcacaccacc
     2761 cgcgccggat accaaggaaa gtcgatttcc tgttttccct caatcagctg acccagccag
     2821 ctttgcagcg tcggcaaatc gactttgtcc gcgcggttga ccaaattctc cgccaaaatg
     2881 cggctcgtgt aaggcagctt gtcgtaagag ccgggcttga tgtcctcaca cgccgcacgc
     2941 gcgtcgtagt attccaaatc cgtaccgggc agcggtttgc ggtaacgttg gttggcagcc
     3001 atgtcggttc tcctggggat ctgtttttct tgtggtttga ggtttcagcc gacgtttttg
     3061 gaaggggtcg tctgaaagag tttaaaacat cgaaacaatc atctgataca gcggatttct
     3121 ctcgtctgtc aacaatttca cagccatcga aatgctgatg acaatcagca gcggcttaat
     3181 cagcttcgaa ccgaagcgga cggcaaatct cgcacctaaa ttcgcaccga caaacgcacc
     3241 gaccgccatc gttgccgcaa tcgggaaaat aatcgaaccg tgcagcagga ataccgatag
     3301 cgaaccaaga ttgcaggcaa cgttcgccaa tttggtgtaa gacatcgcgt tcaacagctt
     3361 gcagccgagc aaaacaataa aggcaatcag aaaaaacgag ccgacacccg gtccgaacac
     3421 accgtcgtaa aaacccaaaa gtggtgcaac cgtcagaccg aacagaaaaa aagacattct
     3481 ggctttgcct tccttactgc cgtcgagctt gggcgaaaac acaaaataca gcgcgacaaa
     3541 tatcaacaaa accggcacga ccgccagcag aatatctttg gaaaccaagc tgaccgataa
     3601 tgcaccgacc acgccgcctg caaacgatgc tgccgcaatc gggagacctt tcttccaatc
     3661 aatcaaacct ttgcgtgcaa aagaaaccgt agccgaaaac gtagcagcgg ctgcttgcag
     3721 cttgttggtg gcaattgccg acacgggagg aatacctgcc aacaagagtg caggcagcgt
     3781 aatcaaacca cccccacccg caatcgcatc gataaatccg gcaatcatcg caaccaaacc
     3841 caaagcgagt attatgtata aatcttccat gtttcttatc ctgttacttg caccaataca
     3901 ggattatctt cttctattag gttaaatttt taaacttatt tcagacaacc tttccaatca
     3961 ggcaaggtcg tctgaaatcc tcagctttgc ataccgaaat taaagaacaa actgctattg
     4021 cattcttcaa ttatcgttct tcaatctcca caaacgccaa atcttcaggg cctgtgtagt
     4081 ttgcgctcgg acggatgatt ttgccgtctt tgcgttgctc aagaacgtgt gcgctccaac
     4141 cggttgtacg ggaaattacg aacagcggtg tgaacatagc ggtcggtacg cccaattttt
     4201 ggtaggaaac ggcagagaac cagtccagat tcgggaacat ttttttctct tcccacatca
     4261 cgctttccaa acgttcggca atgtcaaaga ggcgcatatc gccggtttct ttgctcaaac
     4321 cgcgtgccac ttctttaatg acaacgttgc gagggtcgga aatggtgtac actggatgac
     4381 cgaaaccgat cacgatttct ttgcggccga tgcgttcgcg gatgtctgct tcggcttcgt
     4441 cggcattgcg gtagcgtttt tgaatatcgt aagccacttc gttcgcgccg ccgtgtttcg
     4501 gacctttcaa cgcgccgatt gctccggtaa tgctggagta catatcagag cctgtaccgg
     4561 caatcacgcg ggcggtgaag gtagaagcgt tgaactcgtg ttcggcatac agaatcagtg
     4621 aaacgtgcat ggctttgatg tgggattcgc ttgggcgttt gccgtgcaac agttgcagga
     4681 aatgaccgcc gatggtctct tcgtcgcttt caacctcaat gcgtttgccg ttgtgcgaat
     4741 attgatacca gtacaagagg atgctgccga ggctggcgat cagtttgtcg gcgatgtcgc
     4801 gcgcttcact ttccggatgg ctttcacgtt caggatgaac gcagcccagc atggatacgc
     4861 cggtacgcat tacgtccatc ggatgggtat gtgcaggcag gctttccaaa actttaatca
     4921 cacggatagg caggccgcgc atggatttga gcttggtttt ataagcggcc agctcgaatt
     4981 tgttgggcag atgaccgtga atcagcaggt gggcgacttc ttcaaactcg catttttgtg
     5041 ccaaatccaa gatgtcgtaa ccgcgatagc tcaaatcgtt gccggtacgg ccaacggtac
     5101 acaaagcggt attaccggcc gcaacgcctg aaagcgcaac ggatttctta ggtttgaagg
     5161 tcggggtttg agtagtttca gtcatggtat ttctcctttg tgtttttatg ggtttcgggt
     5221 tttcagacga ccgatgcgga tttgttgaaa gaccgtctga aagcggtaaa tcatttttga
     5281 aacaatttat ccagtttttg ctcgaaggca tgatagttca gatgctcgta cagctcggca
     5341 cgggtttgca tactgtccac caccgccgcc tgagtgccat cgcgcataat cgcttcgtaa
     5401 acattcagag cggctttgct tgctgcacgg aacgatgaca gcggatacag caccagcgac
     5461 acgccgtttt cagccagctc gctttgggta taaagcggag tggaaccaaa ctcggtaatg
     5521 ttcgccaaca cgggcacttt caccgcatct gcaaattggc ggtacatgtt caaatcggtc
     5581 atggcttcag ggaaaatcat gtccgcaccg gcttcgacac aagcttgggc gcgttcgata
     5641 gcggcatcca aaccttctac cgccagcgca tcggtacgcg ccataatcac gaagttctca
     5701 tcaacgcgcg catctacggc agctttgata cggtcgacca tttcatcttt agatacaatg
     5761 gctttgttcg gacggtggcc gcagcgtttt tgcgctacct gatcttcgat gtgaaccgct
     5821 gcaacaccgg cgcgttcaaa gttgcgaatg gtacgggcaa tattgaatgc accgccccaa
     5881 cccacatcga tgtccaccag cagaggcgta tccacgttgt ccgtaatgcg tcgtgcgtcg
     5941 atcagcacat cttccattgt ggtaatgccc aaatcaggga taccgcaaga acaggctgcc
     6001 acgccgccgc cggacagata gatggctttg aaaccgcttt gggtggccaa tcgtgcaaaa
     6061 taagcattga cgcaaccggc gacggcaagc ggattcgatt ctttcacggc ttggcggaaa
     6121 cgtgctccgg cagagtgttg actcatcata tttctccttt atagactttt tttcagtatg
     6181 gacaggcttc catcacattc ggacggcaaa acacagccat ccgacgcgtc gggcatccca
     6241 atccaatcat taaaaatata tgggaaaaat tatcttattg atatttaaaa cgaatcaaag
     6301 aaaaacagca gaccgttcgg aattatgcgg caaaaccgca gacaagaaga aaacaagggg
     6361 attattcaga aaaggggaaa acatctgaat tggtttcata gtaatgttcc tttgtagtgt
     6421 tatgtagttt tatttttcga taatctaaaa cacaagactg aaaatgtcaa acatttttaa
     6481 ggagactcat tacataattt taatatttta ttttaattcc atgattaaac tagattaatt
     6541 ttgaattatt tgtttcaata aaattgtcag gaaatcaaga aaaattttcg ataatcaaaa
     6601 tcctgaaaac ttataaaata ttttatacat aaaaacaatg tattattttt aagcaatccg
     6661 atgtatttta ttatgtattt gatttttata taatttaata aaaattaaat atatcggcaa
     6721 acaaaatgcc gtctgaaaac ctttcagacg gcatttttaa tatcgaatca atcagcccaa
     6781 ataccagaat ttcagtgccc acaatacggc aacaatccat accataggcg gaacgtcttt
     6841 ggtgcggcgg cataaaagtt taaccacggc atagctgatg aagccgaagg cgatgccgtc
     6901 tgcaatcgaa taggtaaacg gcatgaagac aatggtcagg aatgcgggtg cggcttccgt
     6961 catatcgtcc caatcaatat ccctcgcact gcggagcatc tgcgtgccga cataaagcag
     7021 ggcgggcgcg gtggcaaaag cgggaacact tttcgccaaa ggtgaaaaca tcaggcaggc
     7081 gagcatcaat acgccgactg taaccgccgt caggccggtc cgtccgcctg ccgatacgcc
     7141 cgccgcgctt tccacataag gcgtggtgga agaagtaccc aaagccgcac ctgccacaat
     7201 ggcggtagag tctgcaagca gtgcgcgttt caggcggggc agcttaccgt ccaccagcag
     7261 cccggcacgg tgggatatgc cgaccagcgt tccggtactg tcaaatagat cgaccaagaa
     7321 gaagacgaaa atcacactga ccatgctgac ggtaaacagg ccttcaaaat ccatctgcat
     7381 aaaagtcggc gcaatgctcg gtacttcgcc gatgatgccg tgaaattcat tcaaacccat
     7441 caggctggca atgacggtaa tggtcaagat ggtgatgatg attgcgcctt gaacgcggaa
     7501 atgtcccaat acgaccacca tagcaaaacc gaacagtgcc aacaacgcgg acggctgatg
     7561 aatatcgccc aaaccgacca aggttgccgg attggcaacg ataatgcctg cgcctttcag
     7621 ggaaatcagt gccaaaaaca aaccgatacc ggcagcaatc gacattttca aacccatagg
     7681 cagtgcgttg accagcattt ccctgacttt aaaaaagctg aacaggataa aaatcagacc
     7741 ggagatgaac accgcaccca acgcaacctg ccaaggcacg cccataccct taacgacggc
     7801 aaaggtgaaa taggcattca gccccatccc cggtgcgagt gcaatcggat agttgccgac
     7861 aaaacccata acaaaacagc cgatggcaga cgcgatacag gtagcgacga ataccgcccc
     7921 catatccatg ccggtctcgc ccaaaatcag agggttgacg ataacgatgt agcacatcgt
     7981 caaaaaagtt gtcaaacccg ccatcaactc ggtacgcacc gtcgtaccgt ttgccttcag
     8041 cttaaaaatc ccgtccaaca gtgtttgttt tgaagtgtcc atatctgaaa gtctgtatca
     8101 agtggaaaaa tctgaatccg ccgaccgcaa accgtacgaa attgcaacat cttgaaaaac
     8161 atacctccca aaccggacaa accgattcgg gaggcagcag gtttacgggc aaacagcaca
     8221 ttatttgtct gaattgtgtt gacgacgcaa aatcgcaggg atttcaaagt cgtcaagtac
     8281 ggactgattg tcgaaatccg cagcggtaag gttcatcgtg cggataccgc gattggtgcg
     8341 gatcatacct tcgacattgt ggctttgctc ctgtttggac ggagcaaccg cttctacctc
     8401 ccttgccgga acaaaatcga ccgcgccttt ttctttcaga ccggtagcga taatggtaat
     8461 ccggatggca tcttcgctca tggtctcgtc ttcagccgca ccgaatttgc attccaaatc
     8521 gggatgcgcg ctttggttga cgattttcat gacttcggac aactcggaca ttttcaagca
     8581 acccggagca gtcgtaatat tgaccagcac accgcgcgct ccgtccaagg ttacatcgtc
     8641 cagcagcgga ctggaaatgg cctggtcggt cgccatacgc gcacggtcga taccttgggc
     8701 ataacccgaa cccatcatag cgataccgcg gttgctcatc acggttttca cgtcggcaaa
     8761 gtcgaggttg atgatttcgc tcgggcaagt taccacttcg gaaatgcctg cgaccgcatc
     8821 gcgcaataca ttgtcggcgg cacggaaggc ttcgcgcatc gttacgtctt cacccaatgc
     8881 agtcatcagt ttgtcgttcg ggatgatgat cagcgaatcg acgtgttctt tcaactgttc
     8941 taaccctgcc tgtgcgacat ggacgcgctt accttcatat gcgaacggtc gggtaaccac
     9001 ggcaacggtc agaatgccca aagacttggc aatctcagca acaaccggcg cggaaccggt
     9061 accggtaccg ccgcccatac cggtcgtgat aaacagcata ttcgcaccgc gaatggcttc
     9121 ttcaatggct tcccggtctt cctgggctgc cgcacggccg atatcgggat tcgcgcccgc
     9181 gcccaaaccg cgtgtcagat tcgtacccaa ctggattctc ttcgccgcat ggttttttgc
     9241 cagagactgc gcatccgtat tggcactgat aaactccaca ccgcgcacat tgttggcaac
     9301 catgttattg attgcattgc aaccgccgcc gcccaagccg attactttaa tcaccgcagg
     9361 gctgactgcc gattctgcca cgtcgtaaac aaattccatt caaaaactcc tgctcgcccc
     9421 attcagagga cggtttaaat aaattattat tcattatata agaagtatct tgctgctggc
     9481 aaaatacttc tcacctgtca aacggcaatc cacctgttca gaagctgttt tcaatccacc
     9541 gtttcaatct tgccaacaaa ccgccgcccc cttccctctc ttgcactgca ccgttttccg
     9601 gctgcggcaa gtttccttcc agcttgcatg ctgcatgaag cagcccgata gcggtagaaa
     9661 aacgcggtgt gcggacgcgg tcggacaaac cgcccatttc ttggggtgca ccggtgcgta
     9721 caggcaaatc gaagattttt tcggcaaatt ccacaatccc ggtcatcatg gacacaccgc
     9781 cggtcagaac gatacccgca ttcagcactt ctttggggaa acccgatttt tgcagctcgc
     9841 ccagcactac gccaaaaatc tcctgaatcc gtgcactgat gattgctgcc agaaccttac
     9901 tggaaacctg acgcgatgtc cggtcaccca cgcccggaac ttcaatcatc tcacccaagc
     9961 cttccgtatc gcatgatgcc acgccataat ggattttaat gtactcggcg gcatcgagag
    10021 gtgttctcaa cgatttggac aaatctttgg taatcagatt accaccggcc ggaataacgg
    10081 acgtatggcg gatggcaccg ttcatataaa cggcaatatc ggtcgttccg ccaccaatgt
    10141 cgatgacgca tacgccgagg tctttttcat cttcagtcag caccgcctgc ccgcttgcca
    10201 acggctgaag catgatctga tcgcttttca aaccgcaccg ctcgatacat ttttggacat
    10261 tctgcactgc cgtacttgca ccggtaatga tgtgcacccg cgtatccaga cgcacaccgc
    10321 tcataccgat gggctccctc acgccaagtt gggtgtcgat aatgtagtct tgaaccacgg
    10381 catcgagaat tttttgatcg ggcgggatat tgattgcctt tgccgtttca atggcgcggt
    10441 cgatgtctgc ctgcgtgact tccccgtctt taattttaac cacaccttgc gaattgagac
    10501 tgcggatgtg gttgcctgcg atacctgtgg taacgtgggt aattttggta tccgccatca
    10561 gctcggcatc attgaccgcc tgcctgatgg cttggacggt ggcatcgata ttggttacca
    10621 tgcccgcgcg caagccccgt gaaggagtct gccccaaacc gacgatgttg attttgtcgt
    10681 catcttgaac ttccccgatc agtgcgagga ctttagacgt accgatatcc agtacgctga
    10741 tgtatctttg ctgctgttcc attgttcgtc tgctcttaaa actgattgaa atttgcgtcg
    10801 caccatttca gacggcacgg ccgtaatctg tccgatacct gttcccacta ttcttcggat
    10861 tctttttcgg gtaaaccgtc ggaagcatag cggactgaaa atccgtcctt atacctcata
    10921 tccacatagg ataaccgatt tttattttta cgcaacagat gctgccacgc ttcggtaaaa
    10981 agccggaggc gtttcatctc gttttcccgt ccgagcctga cggtgatgcc gttgtccaaa
    11041 acgacaatcc acgccgaacg tgccgtatag gtcatctctt tgatgcccaa accctgtttt
    11101 gccaaaacag tcgaaaattc gtcataacgg cggagcattt cggcagacgt tccttccgcg
    11161 cctctgaata ccggcattcc gggtctgtcc aagcgggctt caaaaacatt gccttcgccg
    11221 tccaccaagg catggtcgcc ccaacgcgcg accggcttgc gctcggtcag gacgacctca
    11281 accgtgtcgg gaaaacggcg gcgcaccatg accgacgcaa tccacggata ccggcggtag
    11341 gcctcctgtg cgccattgat gtccgtcctc aaaatattcc catggatgta ttctttcgcc
    11401 aaactgccca atgctttctt atcggaataa accaggttgc ccttcagcga cacctgcttg
    11461 acgggcagat gattcgaatt gtaaaaccaa accagcccgg acgcagcaag cagcatcgcc
    11521 atcatgacaa gcagccagcg cgtcagccgt tccatcgctt cggcattatc ccacatgtgc
    11581 ggtcttcaaa atttcaatac ataaatcggc aaaacccacg cccataacgg cagcggattt
    11641 cggtactaaa ctatggccgg tcataccggg cagggtgttg atttccaaca gatagagttt
    11701 gccgtcggta tctttgagga aatcgacgcg cacgcagcct tccgcaccga ttgcctgcgc
    11761 gccgcgaacc gccagttcgc gcatcaggct ttcttcggct tcggtcagat cttccgaagg
    11821 acattgataa atggtgtcgt tgcggttgta cttggcttcg tagtcataaa actcggtcgc
    11881 ggggatgatg tgtatgccgg gcaggccttt gccgttcaac acagggcagg aatattcgcc
    11941 gccgccgata aaccgttcgg caatgatttc gccctgaaag tgtttcaatt cttcgtaaac
    12001 gcttttcaga cggccttttc ctttgacttt taccacgcct acgctgctgc cttcggccgc
    12061 cggtttcaca aacatcggca ggcccaattt ttcttcgacg gcatcgaaat cagtgtcgtc
    12121 gtgcaggacg gcgaactcgg gaacgggcaa tcccaatgcc tgccaaatca gtttgcagcg
    12181 gtatttgtcc atgccgatgg cggatgcggc gacaccgctg ccggtatagg gaatgcccaa
    12241 cagttccaat gcaccctgaa cagccccgtc ttcgccgtaa gtaccgtgaa ggatgttgaa
    12301 tgccgtctga aaaccttgtg ccttcaattc agacaatggg gtttccttgg gatcgaaggc
    12361 gtatgcgtct atgcctttgc tttttaaagc attcaaaatg gcggtgccgc tgtccagcga
    12421 gatttctcgt tcgctggaaa aaccgcccat caatacggcc actttgccaa aattctgcat
    12481 tgtttttgtt ctttcctgat tgctttatgc ttgttgccag aggtcgtctg aaacctgatt
    12541 tgcggtttca gacgaccttt atatgatgtt ccgtctgtca ggcgggtgtg cctcaaatct
    12601 gtttcgacaa ttccagcagc gcggcgggga cgcggttgat gcttcccgca cccatattca
    12661 acacgatgtc gccgtcctgc aaaacgttca acagcatttc gggcagatcg gcaacgtttt
    12721 cgcagtaaat cggctcgagt ttgcccaaca cgcggatggc gcgggcaaga gcgcgggaat
    12781 cggcggcggc aatcggctct tcaccggcgg cataaacttc ggtcagcacc agcgcgtcaa
    12841 cggtattgag gactttggta aagtcttcaa acaaatcgcg cgtgcgggta tagcggtgcg
    12901 gctggaaggc gagtaccaaa cgtttttccg gatacgcgcc gcgtgcggcg gaaagggtcg
    12961 ccgccatttc gacggggtgg tgtccgtagt cgtccaccaa gagcgcggtt ccaccgtttg
    13021 gcaacttgat gtcgccgtat ttttggaagc ggcggccgac accttcaaag ccgagcaagc
    13081 ctttttggat cgcttcaacc gatgcgccga cttccagcgc cacgccgatg gctgccaatg
    13141 cgttcagcac gttgtgtctg ccgggcatat tcagcacgac ttcaaacgac ccctgctcat
    13201 gtcctttcat ttgaacatgg acggtgaatt tcatttgcgc gccgacgttt tcgatgtcgg
    13261 tggcgtagat gtcggcggta tcgtccaaac cgtaagtagc ataaggtttg ctcactttgg
    13321 gcaaaatcgc gcggacgtgt tcgctgtcaa tacacaaaaa ggctttgccg tagaagggca
    13381 tacggtggat gaaatcgata aacgcctgat gcagtttttc gacgctgtgc ccgtaggtat
    13441 ccatatggtc ttcgtcgata ttggtaacga cggacataat cggtgtcagg tgcagaaagg
    13501 atgcatccga ctcgtcggct tcggcaacga tgtattcgcc tttgcccaag cgggcgttgg
    13561 tgcctgcggc gttgagtttg ccgccgataa cgaaagtcgg gtcaagtcct gccgcgccga
    13621 ggatggaggc ggtcaggctg gtggtcgtgg ttttgccgtg cgtgccggca atggcgatgc
    13681 cgtcacggaa gcgcatcaac tccgccaaca tcagggcgcg cggaataacg ggaatttgct
    13741 gctccaacgc agcgacaact tcgggatttt cttttttgac ggcggtagag gtaacgacga
    13801 catccgcacc gttaacgtgt tctgcggtat ggccgggata aacttgaatg cccaggctgc
    13861 ccaaatgctc ggtagcggca tttcgcgcct gatccgaacc ggaaacttta aaacccaaat
    13921 tgtgcaagac ttcggcgata ccgctcatgc cgacgccgcc gataccgaca aaatggatgt
    13981 tggtcactcg atttttcatc ataatgttgc gttccggtgg atttcgatgc gtaaaggcgt
    14041 tattttaaag ggctgaccgt ttgcgcgcca tagttttctg acaaatatat agcggattga
    14101 aataaaaaac atccatgccg tctgaacggc ttttcagacg gcatggttcg gcagtttacg
    14161 ccgcacacgc aatcgcggct tcggcaacgt cgtccgcgct gtgcggcaat gccaaagtac
    14221 gggcgttttc tgcccatttg agacattttt cgcggttcag gctgccgagg atttcggcga
    14281 gtttttccgc cgtcaactgg gtttgcggca acagcaatcc cgcctccgcc tgcaccataa
    14341 aacgcgcgtt ggcggtttga tggtcatcaa cggcgtgagg atacggcact aacaacgcac
    14401 ccaatcccgc cgccgtcaac tcggcaatcg tcagcgcgcc ggcacggcaa atcaccaaat
    14461 cggcatcgcg gtaggcggac accatatcgg taataaattc cacgcattcc gcttgcacgc
    14521 ccagcgcgtc gtaatccgcc tgcaagctgc ccagcttgcc ccgtcccgat tggtggtaca
    14581 tctgcggacg cgcattgtcg ggcagcaaag ccaatgcctg cggtacggtt ttgttcaaaa
    14641 cgtccgcgcc caaactgccg ccgaccacca aaattttcag acggccttca cgcccttgga
    14701 agcgttcggc aggcacgggc aggttgctaa tatcggcgcg gacggggttg ccgaccaagc
    14761 cgccttcgtg gctgaacgct ttcggaaaag cgtacaacac ccgcttcgcc cagcgcgaca
    14821 ggtggcggtt ggacaaacct gccacggcgt tttgctcgtg aatcacaatc ggcacgccta
    14881 ataacttcgc cgccaaaccg ccggggaagg taacgaagcc gccgaagccg atgacgcact
    14941 cgacacggtg tttgcggata atccgctgcg cttcgcggac ggtttgatac aaagtaaacg
    15001 gcagcatcag cttgcgtttg atgccgttgc cgcgcacgcc tttaatcgcc agcgtttcca
    15061 agcgtatgcc gtattgcggc acgatacgct cttccatcga atccttgctg cccagccaaa
    15121 tcacatgatg gccgcgcgcg cgcaatgaat ccgccaccgc cagcgcgggg aaaatatgtc
    15181 cgcccgttcc gcccgccatc agcataaagg ttttaccgcc catgatttac tccacccgat
    15241 aaccgcgcat tttccggcgg ttttcataat ctatacgcaa cagcagcatc atgctgatca
    15301 gcatgaaaaa gactgacgaa ccgccatagg acatcaacgg cagcgtcaga cctttggtcg
    15361 gcaaagcacc gatgttcaca ccgatattga agaaactttg gataccgatc caaatgccga
    15421 tacccgaagc gatataggcg ttgaaagtca aacccaaatc gcgcgactgc ttgccgatgg
    15481 aaaacgcccg caccaccagc cagccgtaac agaatatcag cacgcacata ccgaagaaac
    15541 cgaattcttc ggcgatgatg gcaaaaataa aatcggtatg cgcttccggc agaaagccgc
    15601 gtttgctcaa actcgcaccc aaacccatac cgaaccactc tccgcgcccg attgccatca
    15661 gagagtgggt aagctggtag ccggcaccct gcgggtcttt ccacgggtcc aaaaatgcca
    15721 ctacccgctg cacacggtag ggagcggcgg taatcatcag caccatcccg cccaagacgc
    15781 tgcctaccag gacgaaaaaa tatttccacg gcaatcctgc caaaaacagc attccaacgg
    15841 caatgacggt aatgacgaca aacgaaccga aatccggctg taccattatc agcaccaaac
    15901 cgaacgccac cagcataatc ggcaggatga tcgcccggaa acggccgtac atttctaatg
    15961 tttcacgacg tgcctgcgga ttggtggcgg acatgatcag attggccgtc ccccgccaaa
    16021 tcgactgcca acccaaactt tccatgctgc gcaacacttc ttcacggcgc gtgaacaggc
    16081 ttgccaaata aaggatgacc gccagcttga acagctcggt cggctggaaa ttcaacggac
    16141 ccaaaggtat ccaacgggtc gcgccattga tttcgcgccc ggcaatcaat acgactacca
    16201 gcaacaggcc ggatagggca aaaatccacg gcacaagccg ccgccatgtc ctcatcctgc
    16261 aaagaaacca taaaaaaccg ctcgctatca agccggcaac gacgaacccc gcctgtctgg
    16321 tcaaatagaa aaactgatcg ccgccttctt ttgatgccaa atacacagaa gccgaataaa
    16381 tcatcagcag gctgaacgcc gtcatcagca ccaccatcca caaaagcggc gcgtcgaatt
    16441 tcctgccgtc gcgcacaatc ggcctgtcga gcagcagagt gtggacaccg tcgcccactt
    16501 ttaccaatac ttccgaaatc ttcaaaaaaa ccacctgcca gtctgtttgc acctgccgca
    16561 aagggcaaaa atttcagacg gcagacaatg ccgtccgaac atacgataca tcccaaatcg
    16621 gtattctaaa tctttacttg ccgcccaaca atgacggcgt ttgcgtttca gacggtatca
    16681 caaagcctta aacgccccga taaacacttc cgaacggtgc gcatagcctt taaacatatc
    16741 gaagctcgcg caggcggggc tgagcagcac gatatcgccc gcttcggctt gggcatatgc
    16801 cttctgaacc gcttcttcca aagtggcgca gtcggtcata ttcaaaccgc agccgtccaa
    16861 atcgtggcgg atttgcggcg catcgacgcc aatcaggaac acgccttttg ccttgccggc
    16921 aagcgcgtcg cgcaggggcg tgaagtcctg ccctttgccc atgccgccca aaatgacgaa
    16981 tagcggattt tgcaaaccgg caatcgcagc ggcggttgca ccaacgttcg tgcctttgct
    17041 gtcgtcgatg aacaccacgc cgtttttctc gccgattttt tccacgcggt gcggcaggcc
    17101 ttgaaaggtt ttgacgtgtt cgagcaattc atttcgcggc aaaccgacgg cttcgcacaa
    17161 ggcgacggca gccatcacgt tggcggcatt gtgcaggcct tgcaacggaa tgtcttgcgt
    17221 ggcaatcaaa tcttcattgc cttgtttcag gcggcctgtc tcgcgttcca accaaaaatc
    17281 ggcttcgtgt tccaacgaaa accattttac ctcgcgcccg gcgcgcttca tggcacaaca
    17341 gaacgtatcg tccgcattca aaacctgaac gccatcgcca cggaaaatct tgtccttggt
    17401 atgcgcgtag tcgagcaggt cgtcgtagcg gtcgagatgg tcttcggaaa tgttcagcac
    17461 cgtcgccgca gtcgggcgca ggctttcggt gttttccagt tggaagctgg aaagctccaa
    17521 cacccacacg tccgcctttt tgccttcacg ctgcaattcc gcctctaaaa ccggcgtgcc
    17581 gatattgccc gcgataacgg tatccagccc gcacttgatg cagagatagc cgactaggct
    17641 cgttaccgtg gttttgccgt tgctgccggt aatcgcaatt accttgtcgc cccggcggtt
    17701 cacaatgtcc gccagcaatt cgatgtcgcc caacacgcgt ccgccgtttt gcttgaacgc
    17761 ctcaatatcc ggctgccgct cgctgatgcc ggggctgagt gccagaatat cgaaaccgtt
    17821 gtccagcgca tctttcagac ggcccgtgta aaacaccaac ccgtcaaaca tcttaccgat
    17881 ttgcgacacg cgttccggct tcagctccgc atcatacgca gcaacctccg cgccgttttt
    17941 gcgcaggtag gcaatcatgg aaatacccgt accgccgagt ccggcgacga ggattttttt
    18001 gttttgaaaa gtcattttgg tttgtcctaa aacaaatcat attgagcagg agatgtccgc
    18061 ccctgcccaa gccgctttca gacggcatcg cgagctgttc aataacccgc tttcaggcgt
    18121 tggtcattgt cgcagctgtc ttggtatccg ttttgacaag ccttgccaag ccattcttgt
    18181 gcaagggcgc ggtcttggcg cacgccgcgt ctttcggcat acatcacgcc caaattgttt
    18241 tgggctgctg catttccctg tgctgccgcc tgcaagtttt cccgaaaatc cgatacgtca
    18301 tccgcccaca ccgcttggtt caagcccaag gcaatcaggg cggcggcaag ccatttgact
    18361 gtctgtttca tggttttact tctgttttag tataagccgc cgttaacgat agggctgggc
    18421 ggattgtcgc cgcaggttta ttgcgcgttc aaatgccgtc tgaaaggtgt tcagacggca
    18481 taggttcagc ggattttgag ggtactcaaa ccgatcaaca ccaagacgat ggtaataatc
    18541 caaaagcgga cgacgacttg ggtttctttc cagccttttt gttcgtagtg gtgatggatg
    18601 ggcgccatca ggaagatgcg ttttttggtt ttcttatacc agccgacctg aagcataacg
    18661 gatacggctt ctacgacaaa taatccgccc ataatgacga ggacaaactc ttggcggacg
    18721 atgacggcga cggtaccgag cgcggcaccc aatgccaatg caccgacatc gcccataaag
    18781 acttgcgcgg gataggcgtt aaaccacaag aaaccgaggc acgcgccgca catggcggta
    18841 cagaaaatca ccacttcgtt tgcgccggca acgtaaggta attgcaggta ttgggcaaat
    18901 tgtgagtggc cgctggcata ggcgaagatg gcgaggccgg cggcaacgag gacgacgggg
    18961 aaggtcgcaa ggccgtccaa gccgtcggtg aggttgacgg cattggatgt gccgacgatg
    19021 gtcaggtaag acaacaccaa aaagccgacc acgcccagcg gcagggcgat ttgtttgaag
    19081 aacgggacaa tcaaaatatt gttggcggaa ttggcggcaa ggtaaaacaa tgccaaaccg
    19141 gcgataatgg caacgcttga ctgccacacc attttgaatt tggcggacac gccgttgggg
    19201 tctttataga cgactttgcg ccagtcgtcg taaaaaccga gtgcgcccgt ggcgagcaat
    19261 acgcccaaga gaatccagat atacgggttt gcccagttgc cccacaacag ggtggacacg
    19321 gtaatggcgg tcagaatcag cgaaccgccc atcgtcggcg tgccgttttt gacgaggtgg
    19381 gtttgcggac cgtcggtacg cactgcctgc ccgcatttga gcgcggtcag cctgcgtatc
    19441 gtccacgggc cgaacatcag ggaaaacgcc aaggcggtca acgccgccat gacggcgcgg
    19501 aatgtggtgt attgaaaaat attcagaccg gttaaccagt tgctgaaatg tgcgagccat
    19561 aaaaacatgg ggcttccttt ttttgttttt gtcgttacat gggcttgtgc ccgtttgagg
    19621 ttggcttgcc gcccggcaag gtttcggacg acttttgcag attaacgtgc agagactttc
    19681 ccaatcacgc aaatcgggta actccccgga tttttgccgc cacccaagtc taaacataaa
    19741 tcttcataaa gatacgcctg cgtctgcatt ccggcataaa acgcgccgac cagcgcggca
    19801 atcaacaata aggctttaaa aaaacgtcga cttttcattt tcacttatcc tccaatgcct
    19861 cgaccacttc ttccatctgc ataaagcgcg aacctttcac caacacggtg gcgcgttcgg
    19921 gcaaatcgtg gcgcaacact tgaatcaacg ggtctttggc ggcgaaccac aaaccgtccg
    19981 cgccaaattt ttccgccgct tcgacgctgt tgtcgccgac aaaataagcc gcttcgatgc
    20041 cttggtcgcg ggcgtacgcg ccgacttcgg cgtgcatggc ggcggcttcg tcctcaccca
    20101 gttcgcccat atcgcccatc acgaaaatac gcggcgcagg catacgcgcc aacacgtcaa
    20161 ccgcagcttt catgctgtca gggttcgcat tataagtatc gtcaatcagg gttgcgccct
    20221 tgattccgga tttgacgttc agacggcctt tgatattgct gaagcctttc aaaccttccg
    20281 ccacatcgtt caaactcaaa cctgcagcca aagacagcgc ggcggcggcg gcggcgttgt
    20341 ggacattgtg gcggccggga acgggcagaa ccacggctgc gcactcgttg ccgcacacca
    20401 aatcaaattc gcacgacaac ggtttcagca cgatattttc cgcgtggaca tcgccgctat
    20461 cgatgccgaa agtgcgcgta ttcaaattaa gcgttgccgt tttgaagaca gccatattgg
    20521 catcttcttg aggaatcagc gccatgccgt ctgaacataa gccttgataa atctcgcttt
    20581 tcgctttggc aatatcgccc actccgtcga aaccgcagcc gacatgggcg cgcatggcgt
    20641 tgttgaccaa tgcggcatcg ggtttggcga tttgtgtcaa aaccgccagt tcgccaaaat
    20701 ggttcatacc catttcaatc acggcatagc ggtgtttttc gtttaatttc aacaaagtca
    20761 acggcaatcc gatgtggttg ttgaagttgc ctgccgtcgc caaaacggca ttatcgccga
    20821 aacggcggcg caataccgca gccaacattt ccttcaccgt cgtcttgccg cccgagccgg
    20881 taataccgaa cacaaacggg ttcacattct cgcgccacgc cttcgccaac atttgcaacg
    20941 cggtaagcgt gtcatcgact ttcaacgcgc catccattgc aacgcaatct tcgcgcgaaa
    21001 ccacaaccgc cgccgcaccc gcagccaata cgtcttcaac aaaatcatgc gcatcaaacc
    21061 gcccgcccgc caatgcgaaa aacacatcgc ccgcgcggat gtcgcggctg tcggttacga
    21121 tgcgcgacac gggtttgctt tcagacggca tcggaagctt gagggcttgg cagatgaaat
    21181 ttaggtccag tggtttcata tttactttcg ttaatattcg ggcggcggac acatcggtag
    21241 cggctgattt ttttatcgcc tgttttgctg tggtaaaaca cagattattt tcccattctc
    21301 attcggcatt ttttctgtac gtatcatttt ttagacgtat ttttagtcga tttgcctttt
    21361 cccgcatacc acggcgcggg gtcgtcgggc agtccgtcga taaaggcaag gttattgcct
    21421 tcgccctgca catcgggaac attcccccaa aaatcatagc cgtcatcggg caactcgtcg
    21481 gtttcgatac ccgtccaact gccgaatccg cgtaaaaaat taaacgcgct gcgcctcact
    21541 ttaatcattt cgcgccgcgt atcatcggac gaaattttaa ccagcggcac gccgtagctt
    21601 tgacgcttcc acgcaccatg tgtcaacgtt cgctcgccgc ctttaccgac atgcatcaaa
    21661 ccgtggtcgg aaaagtaaac caaagaccag ctttctttat tttcattcaa tatcttaact
    21721 gtatcttcta aaaatttatc ggtttgcgcg atggtggaaa catagcagga tattttttca
    21781 gtttgatact gaaaccgccg cgcatcctta tccaaacgtg tgcaaaaatc actgtgcgaa
    21841 cccatcaggt gcatcacaat cagccgaggc ttcgtgcctg catttcctat caaaacccgt
    21901 ttgaacgccg gcaacaaaag gcgatcgctc aaccccgcgc ttttgccata atcacccctt
    21961 tgggtaaacc acggataatc gctgcgtagg gcataggtgg aaatttcgtt ggcaaaatgc
    22021 cccaacattc cttgattaga cagccacgcc gtccgaaaac ccgcctgctt cgccaagctg
    22081 acgatgttat tgttcggttc tcccggcagc cccaaagtct gcggcagcga aagattcgtc
    22141 gcgtgggcgg tcgattggta accgtttatc aacagccctt tggtctgact caaaaaaggc
    22201 gtatcgggca atgggaaacc gtaaacattc atataatccg aacgcgcgct ctcaccgata
    22261 accacaacat aatttttata cttgggcgca acatgacgga tatgccatgt tgacgctttt
    22321 tttgctgctt ccaaaatgtg ggcgcgcttg gcggcatatt gtgccggcgc ggaagccaaa
    22381 tcgtaataca ggtcgaaaat attcaacaac aggccggcat caggttctcg caaatcttta
    22441 tcgctggcga ttttatccat caccgcgcag gacaaaatca aagtcaataa tatagtcagc
    22501 catattttgc tgcggcgttt atagtttttt acgtcagcaa ataccccccc cggtacacaa
    22561 tatctccaaa ctgtcaagcc aaaaatgaaa aataatgcct gcacaaaata aagcgacccg
    22621 ggaagattgc cgacaaattc acgcgcctcg gcaggattgc tttccaatat cgaaccgact
    22681 atctgataag acggcgcacc atacagccag ccgaccggca aatatagggc ggttgtgccg
    22741 acgtaaatca gtaaaacaac tgaagaaacg cgcgggaaac cgcgtgccaa taataataaa
    22801 aatacaaccg aagccaaagc acccacggca gaataacggt agccgtattc atattccaag
    22861 tgataacctg atgcgatggc cgcgccaaaa caaaaggcta aaagcgcgca aatgcccaat
    22921 gtatttttta atgtctgatc catatttttt attcgggcgg attgtttcat acgatgccgt
    22981 ccgaatatcc cattatttac gagttaacaa agcctgtccg acgatttcaa gatcggaaaa
    23041 acggtgcttc acgccttgta catcctgata gttttcatgc cctttgccgg caatcaggat
    23101 gatgtcgttt gcggcggctt gttcaaccgc ataacggatg gcggcggcac ggtcggcttc
    23161 gacgcattcg ggcgcgggaa cggcaggcag gatgtcgttg atgatgtcgt gcggattttc
    23221 caaacgcggg ttgtcgctgg tgacgacgac tttatccgcg ccctgtacgg ctgccgcgcc
    23281 catcagcggg cgtttgccgc gatcgcggtt accgccgcaa ccgaatacgc accacaaagc
    23341 cgcaccctgc ggtttgattt cctgcaaggt ggcgagtgct ttttccaatg cgtcgggcgt
    23401 atgggcataa tcgacaacga ccaaaggttt gccgctgttc atgatgcagt ccatgcgccc
    23461 tgaagcggga cggatttttg ccagcacatc caatacttta tcaagcggat agccgttggc
    23521 gcacagcaag gcgatgcagg cggcgaggtt ttgcgcgttg aaccgtccga gcaggcgcgt
    23581 gcggcatttc ccttcgcccc acggggtttg gaatacggct gctatgccgt ctgaagaggc
    23641 ggtgaaatcg ataatgcgga tgtcggcgtg ttcgctgaag ccgtagctat aaacggccaa
    23701 atcgggacag tcttttttca gacgacctac gagttccgcg ccgtattcgt catccacgtt
    23761 gatgactgcg tgtttcaagc cgtgccagta aaacaggcgc gacttgatgg caccgtaggc
    23821 ttccatcgtg ccgtggtagt cgaggtggtc gcgggtgagg ttggtaaaga ttgcgctgcg
    23881 gaatgacacg ccgttgacgc gcgactggtc aagcccgtgg ctggagactt ccatcgcggc
    23941 gactgttgcg ccttgttgac ggaaacggta gagcagggtt tggacatcga cgggggcggg
    24001 tgtggtatgc gtggtttctt ccaatgcacc ccaaaagccg ttgccgaccg tgccgacaat
    24061 ggcggttttt tcgcccaaca aatcggcagc ttgcgccagc cattgcgtga tggaggtttt
    24121 gccgttggtt ccggttacgc cccagaattt gagaccgtct gaaacgttgc cgtaaacttg
    24181 cgccgccaat atgccggcac ggtgtttcaa atctttgatg ccttgattgg ggactttcca
    24241 ttcgggattc cacgcaaatt tgccgtcgtc gtcccaaaaa acaaaagccg cgccgttggc
    24301 aacggcggcg gggatataac tgcgtccgtc ggcatattcg cccggacagg caacgaaaat
    24361 atcgccttgt ttgatttggc ggctgtctga atgcaacaaa cgccctgccg cgtttgcaca
    24421 cgacagggtc gggatgccgg tttcagccaa aggggttaac ttgctgaaca taaaacaatc
    24481 tcgttgatac tcggattaag acggtgtttt gacggctgcg gcggtcaggg gcttggtcgg
    24541 ggaaatgccc aagatgttca ggctgccgcc cataattttt ttgaagggcg gccctgccac
    24601 tacgccgccg taataaccgt gggcagtcgg ttcgtcaatg gttaccgcca caatcacacg
    24661 ggggtttttg gcgggggcaa aaccgataaa ggtagcgatg tgtttgttgt cggcataacg
    24721 cccgttgacg aacttgcgcg ccgtgccggt tttcgcgccg acatcgaaac cgtccaccgc
    24781 acccgccgta ccggtgccgc ccggctcggt tacggaaacc atcagattgc gtacctcgcg
    24841 cgcggtcgat tctttgaata tgcgtttgcc ttgcggcgca accgcctgtt tttcaaagct
    24901 gaccggcagc aaaacgccgt cgtgcgtcag tgcggtatag gcgcgcgcca attgcagcag
    24961 gctcaattgc aggccgtaac cgaaagacat cgtcgcctgt tcaatgggcc gccacctgcg
    25021 ccaatttctc aacaaacctg cagtttcgcc cggaaagccc gagtgcatac gcacaccgat
    25081 gcccaactca tgatagaagt catacatttc ttcggcaccg aaacgcgcag acagtttgct
    25141 tgtgccgacg ttggacgatt tctgcatgat gccgcgcaca tccaaagagg ggtaaacatg
    25201 ggtatcgcgc acgggagacg gtccgatttt ataaggctgc gtattcagcc gttcgttcaa
    25261 atcggttttg cccgcatcca atgccttcgc aatcacaaac ggtttgattg ccgaaccggg
    25321 ttcgatcata tcggttacgg cacggttgcg ccgctgttcg ctgtctgccc ggccgggcct
    25381 gttgggatcg taggcgggcg tattggccaa ggcgaggatt tcccccgtgc gggcatccaa
    25441 aaccaccacc gttccggctt ttgcctgatg gtattcgacc gccttgttca actcttcata
    25501 ggccaaggtc tgaatcctct gatcgaggga aaggatgatg tctttgccgt ttttcggggc
    25561 tttattgcgc ggggagtcca agctgtccac aatattgccc tgccggtccc gcaaaacgac
    25621 ttccgcgccg tcttcgccat gcaggctgtc ttcaagcgaa agttccaaac cttcctgacc
    25681 tttgccgtca atatcggtaa atccgatgac gtgtgcaaac aggttgccca tcgggtaatg
    25741 gcgttttaat tctttttcaa atacaaagtt ttccaaaccc aaggctttga cctcttcggc
    25801 aaccttggga tcgagctgcc gcttaatcca gataaacgac ttgcctttct gttcgagctt
    25861 gttcctcaaa acatcaaccg gcacatcgac aagctcggac aggcgttcca attgtgcggc
    25921 agacggcatt tccttcatct ctttaggcac ggcaaacagg gactccgtcg gcgcactcaa
    25981 cgccaaaacc gcaccgttcc ggtccgaaac cgtaccgcgt gtagccggca atgtttgagt
    26041 ccgcacaatc cggttgtcgc cctgttcttt caaaaagtta tacgttaccg tctgcagata
    26101 cagtccgcga gcaatcagac cggcaaacaa gaccgctatt gccatcagga cgaagctgat
    26161 ccgtccgtta ctggtcatcg gctttttgac ctgctcttct ttgggcagca tccgaggctt
    26221 atattcgctc ttaatcaaca tttttacttc tcgttattat tatcctgacg caggaatccg
    26281 attccggcac acaggctgct tctatctttg atgctccacc ataaaggtat tgcccgaaac
    26341 cggcggatgg aggttttgtt tttctgccgc cgccctgatc gcttcgtggt tcgccaaacg
    26401 cgcctgttgc agcctcattt gcgcataatc ctgctccaag gcgatttcct gttttttcgc
    26461 cttatccaaa gctgtgaagt tgagcctgta ctggttttgc tgcatcacaa cggaaaaagc
    26521 ggaaacgcac accgcaagca gcagaaggaa attcaatttg ttcattgcca ttcagacgtt
    26581 tttctctgtg attgttccgg tatcggaccg gcagtccgct ccgccacacg caaaaccgca
    26641 cttctcgccc tcggattggc ggcaatttcc gcctcacccg gctttaatgc cctgcccacg
    26701 attttcaggg gcagctcggg caaatccgct tccctgaccg ccgcccagcg cggcaggggc
    26761 gcgtgttgcg aatatttttt gacaaactgc ttcacaatgc ggtcttccaa cgaatggaaa
    26821 gcaatgaccg ccaaacgtcc gccctctttc agacgacaca tgacctgcgg caatactgcc
    26881 cctacttctt caagctcgcg gttaataaag atgcggattg cctggaaggt gcgcgtcgca
    26941 ggatcctgcc cccgctcgcg agtacggacg ttttgtgcca cgatctgcgc cagcttgcgg
    27001 gttgtatcga ttggactttc cgcccgttgc gcaacaatgg cgcgcgcaat ctggcggcta
    27061 aaccgctctt caccataatt cttgattacc tcgtgcaaat cctgttccga cgcaaccgct
    27121 atccactctg cggcagacat accgcgcgtc gtatccatac gcatatccaa aggggcatcg
    27181 aaacgaaagc tgaaaccgcg gctgccgtca tcgatttgcg gagacgaaat ccccaaatca
    27241 aacagcgcac cgtccacctt gccgataccc aaaccgtcca atgccgtctg aaacgaagca
    27301 aaaccgccat gcacgacacc gacccgtttg tccgaacgcg ccagctcttc tgccacagca
    27361 atcgcctgag ggtctttgtc gaaaacaatc aaccgccccg catcgcccaa acgcgacaaa
    27421 atcagccggg aatgccctcc cctgccgaac gtaccgtcca catagacacc gtcttcgcgc
    27481 acggcaagcg catccaccgc ctcattcagc aagaccgtga tatgccggta actttctgct
    27541 ccactcacaa ttgcaaatcc gtctgaccca actggaaggc aagttcgtca ggatcgtcat
    27601 ccaaagcctg aaccatctca gcctcccact gctcgcgacc ccaaagctcc aaacggttgg
    27661 cacgaccgac caaaacgact tcacggtcga aatccaccct cttcctcagt ccggcaggaa
    27721 ccagcacccg gccggcgctg tcccattcca aaatttccgc gttatgcagc agaagatttt
    27781 gaaaccgccg taaaacaggg ttatccgcca cttttaagtt taaaagttgc gccgcaacct
    27841 tttcccactc cgcaacaggg tacatcaaca gcttgtgttt cgactcgagc gttaccacta
    27901 cggcaggcgt atagaggcgc gacagaatgt cacggaattt ggcaggaaca gccaaccgcc
    27961 ccttactgtc gatgcttaat tcgtgtgcgc cgccgaacat gacatgtccc aagccgaaat
    28021 caaaatcaca agggtaaaag agacactttg ccccacaatt ccccaccaat cgacactata
    28081 agaaatttta aacactcggt caaatcaggg catgaaaact cattaacata tctgaaattt
    28141 ttattccttt taaaaacaat aagataaaaa atgacgacaa cggcacggcg ggtgcggtac
    28201 agaataatcg aaccaataaa caactatata tgattaattt aataatataa acacaatata
    28261 tagtattaag ataaagccat gacagcaccc gtaccaacgt gtaatatgtc gggaaatcca
    28321 ataaatttac acaagctaac acttatcatg cccctccctc tcccctctcc cgaagcacgg
    28381 caatcctcgc tcaaactgca aaccctcatt gccgaaaaaa tcggcaaaca cggcaactgg
    28441 attccatttt cacgttttat ggaattggtt ttatacgctc cgcaatacgg ctactacacc
    28501 ggcggcagcc ataaaatcgg caataccggg gattttatta ccgcaccgac cctcacctct
    28561 ctgtttgcac agacactggc acgccaactt caagaacttc tatcccaaac ggcgggcaat
    28621 atctatgaat tcggcgcggg aaccggacag cttgccgccg atttgttggg cagcatttcg
    28681 gacggcatca gccgttacta tattattgaa atatcgccgg agctggcagc acgtcagaaa
    28741 aacctgattc aagcccgcgc accggaagca tctcaaaaag ttgtccactt gaccgcactt
    28801 cccgaagcgt ttgacggcat catcatcggc aacgaagtac tcgatgccat gcctgtcgaa
    28861 atcgtccgta aaaatgaagg cggctcattc gagcatgtcg gcgtttgcct agataatgac
    28921 cgttttacct attcggcacg accgctgcac gacttacagc tatctgcctt ggcttccctc
    28981 tattttcctc aaacagatta tccctacacc agcgaactac atccgcaaca atatgccttt
    29041 atccgcaccc ttgcctccag actcgaacac ggctgcatga tattcatcga ctacggtttt
    29101 gatgcagcgc agtattacca ccctcaacgc aatcaaggta ctctgatcgg acactaccga
    29161 catcacgtta tccacaatcc ttttgacttc atcggattgg ccgacctgac cgcgcatgtc
    29221 aactttaccg acattgccca agccgggacg gatgccggat tagatttgat aggttacctt
    29281 ccccaatccc atttcttatt gaacctgggc attaccgagc tattggcaca gacggggaaa
    29341 acgaattcgg cagcctatat ccgtgaagct gctgccgttc agaaactgat tgaccagcat
    29401 gaaatgggcg aactgtttaa agtcatcgca ttcggaaaaa atatcggcat cgactgggca
    29461 ggattccgct tcggcgacat ctgccacaaa ctctaaccct catgccgcct gaatccgctt
    29521 cagacggcat aaacttttta acatttaaaa acagtcaact aattcaaaat taaaaaatac
    29581 ggcttgtcaa aaaaacagaa aaacatataa tagcgtcttc acgaaacggc gaattagctc
    29641 agtcggttag agcagaggaa tcataatcct tgtgtccggg gttcgagtcc ctgattcgcc
    29701 accaaatttt cgggggtata gctcagttgg tagagcgctt gcatggcatg caagaggtca
    29761 gcggttcgat cccgcttacc tccaccagat aaaaaagcac agaccgtaaa aaggtatgtg
    29821 cttttttatt acctgattac cggcaaataa agaataaacc actgccttca aaacaggcaa
    29881 tcgactttaa acctatcgcc ccgcctgtcc tgattttata gtgaattaaa tttaaaccgg
    29941 tacagcgttg gctcgccttg ccgtactatc tgtactgtct gcggcttcgt cgccttgtcc
    30001 tgatttaaat ttaattcact atatcagccc gccagacaaa cccgacccga ataatgtctt
    30061 caggtcgggt ttatggtttc attcccaact tatccagccc gacaaccaca atattatgat
    30121 gacgatgccg aaaacaatgc ggtaataggc aaacgggata tagtttttct tggaaacaaa
    30181 cttcagcagt gctttgaccg ctactaaacc ggaaacaaaa gcggcaataa agcctatcag
    30241 aatcaaaccg acatcatgca gggtgaaaaa ccggtaatgt ttcaggacat cataagccgt
    30301 tgcggcaacc atcatcggaa cggccaagaa aaatgaaaac tccgttgccg ttttccgctc
    30361 gattccccaa agcatcccgc ccataatcgt actgcccgaa cgggacgtac ccggaaccag
    30421 tgcaaacact tgggcaacgc cgatcatcaa ggcatcaatc ggacgcaatg catcaacatc
    30481 gacaatttta ggctctgccc ggctttggcg tttctccacc cacaaaataa aaaaaccgcc
    30541 caaaaccagc atgactgcaa cactcaaggg gttaaacaga tactctttga tttgtttgcc
    30601 gaacaacagc cccatcacgg cggcaggtat aaaagcaatg gcaagattaa ggacgaaacg
    30661 gttggctttc cggtcttttc ccacgccatg caacacattg ctgaaacgct gccggtattc
    30721 aaacactacc gccaaaaccg caccgagctg gatggtaatt tcaaaaacct tgtgattgct
    30781 gtgaaaatca atcagattgc cgaacacaat caaatgtccg gtgctggaaa tcggtaaaaa
    30841 ttcggtaaaa ccttctacca agcccatcat cagggctttc aggacaatca gaaaatccat
    30901 tgcttgcgct tctttcggat acgggagttc ggctatttct gtacagcagg ggtctgacgc
    30961 ttgcgttctt ccctgacttt ggcaaccaat tctttaatcg tatgaacacc gccgtcaaag
    31021 ccattattga agataacgcg gtattttccg ccgacgacaa cggtcggcgt actgtcgatg
    31081 cggtactgtt ccgtcaattg ctgcattttt gatgcagcag ccgctgccgc aggagagtcg
    31141 taggcgcgca tcagtttttt gccgtcaaag cctttttgag acaaagccca tttttcggca
    31201 accgacctgt tttccaagcg gattttttgt tcgtaaactg ctttaaacac ggcagggttt
    31261 gcctgatatt tcaaacctga cagcttgacc gcagcagcca ttcttgccag accgagcatt
    31321 tcaggctgcc agaccacgtg ctccgtcctt aaataggcat cagacggcaa tgccttgccc
    31381 agtttcaata acaaaggatc gaaatgatgg caatgtacgc agaaatagcc gaaaaattcc
    31441 aaaacctcaa ttttacccga ctgttcttga ggaatgggtt tatccaacac aagatagtct
    31501 tccccttccg tcagggcata tgcctgcgcg gacaacactg ccgacagcag cagcggcaac
    31561 agatgtttga gcttcataat tatttacttt cgatagaacg gatcaggctg gcgacttcat
    31621 gttttttcaa ctcgtcctgc atttttttca ccgcatcggc agacatattg ccgctttgca
    31681 cccggtaaag cgttttatgt cccgcctgat agccgaccac cttggaagat atgcccaaga
    31741 ttgccagttt ggcacgctgc ccttccgcgc tccggcggtc ggcatacgcg cccatttgca
    31801 gataatgcgt tgcttccgcc ttgtcgggcg ttttcatttt ctgcacttct ttggcagcgg
    31861 cactgcgcgc tttttcgatg ctgccgctgt tgaggatttg ttccggggtc ggtttgggtg
    31921 caactttttc cttctccgcc tttttctctt cttttgaagc ttttttctct gtttctttag
    31981 atggttttac cgcttgtttt ttaaccgttt cggcatcttt cttctgcgct ttttccctga
    32041 cggtttgttc acgctcttcc gtcagcactt tcttacgcac tgcctgtccg tccggctctt
    32101 cccgttccgg ctcgcccgcc ttttcttcaa cctcgtcggc tttatcggca gctttttccg
    32161 catccgactg ctctgcctct gtcgcagcat ccggttcgta caaggcgttt tgatcggccg
    32221 gttcaggttg gatgtcttcc ttaggctggt ttttcggttt caggatttcc gtttctgcag
    32281 gctgcttcga cgaagccggg attttgaacg cattttgacc gctttggttc agataaaaca
    32341 aaataccggc aataatgacc gtcgccagta tcaaaccgaa gaaaaaaccg gacagacctt
    32401 ttccggattg ggaaaatttg ttcataaaca taccttaatg tgtttcagac ggcattaacg
    32461 ccgtttgctt acgggcggat attctaacaa tatcgccata tttgggcaaa acctgcttcc
    32521 attcccgttc ctataaagcg cggcggaaac ctatggcttt catgacgtgg cttctgccga
    32581 cttcttcgtc gcccgccaaa tccgccaatg tacgcgccac gcgcataatg cggtggaagc
    32641 tgcgggcgga aagggagagt ttttccagca ggccgcccaa tgcttcctgc gcttcttttt
    32701 ggatgcaggc ttgggaatcg agttcgctga cgctcaaagc ggcgttgact ttgccctgcc
    32761 gtgcgtattg tttgtcgcgg gcggcgataa cgcgttccaa aacggacgcg ctgctttcgc
    32821 ccgcttcctg ctgcatcagt tcggcggcgg acaggctggg gacttcgatg gtcaaatcga
    32881 tgcggtcgag cagcggcccg gaaatcttgc tgcggtaacg cgcgacgctt tcgggcgtgc
    32941 agcggcaggg tttgacggga tgcccgagat aaccgcacgg gcaggggttc atggcggcaa
    33001 caagttggaa tttggcagga tagacggctt ggcgcgccgc gcgggaaatg tggatttcgc
    33061 cgttttccaa cggttcgcgc aaaacttcca aaactttgcg gtcgaactcg ggcagctcgt
    33121 ccaaaaacaa tacgccgtgg tgcgccaatg aaatctcgcc cggacgcggg tcggaaccgc
    33181 cgccgaccat agccgccgcg ctggcgctgt gatgcggact gcggaaagga cggttgctgt
    33241 cgagttgttg ctggtggttg ggcaggagcg aacgcaatgc ccaaacttct accaattcgt
    33301 cttcggtcag cggcggcagg atgccgggca gccgttggga gagcatagac ttgcccgttc
    33361 ccggcggacc catcatcaag aggctgtgtc cgcctgcggc agcgatttcc aaagcaaggc
    33421 gcgcggtgtg ctgacctttc acatcgcaca aatcaggttg tccgccatgt tcaaacggca
    33481 tctgaggaac ttggcattcg gtttgcgcca aaggttcgat gccgttcaaa tgggcggcga
    33541 cttcgcccaa agagcgcgcg ccgtaaacgg taatgccgcg catcacggcg gcttgtcctg
    33601 cattttcttc aggcaaaaca aatgcacgtt ttgcctgcat accctgccac gccatcgcca
    33661 acgcgccgcg cacggggcgc aacagccccg acagtgccaa ttcccccgca aactcgtatt
    33721 ccgccagttt ttcgggcgca acctgccccg atgcggcaag gatgccgatt gcaatcggca
    33781 aatcgaaacg ccccgactct ttgggcaggt cggcgggggc gaggttgacg gtgatttttt
    33841 tggcggggaa ttcaaaaccg ctttgaataa tggcggcacg gacgcggtcg cgactttctt
    33901 tgacttccgt atcgggcagc ccgacgatgt tgaaatgcgg cagaccgttg gcaaggtggg
    33961 cttccacttc gaccaacggc gcattcatac cgctcaaggc gcggctgtaa accaaggcaa
    34021 gcgacatatt tcagacggcc ttattcgccg gcttcggttt gctgcctgat ttcggcgacg
    34081 gcttcttcgg cagcggcttc agccgcttcc aatgctgccc gttcgggatt ttgcgcggct
    34141 tcgagttttg ccaaacgcgc ttccaaagcc gccagtttgg tacgggtttt gattaaaacc
    34201 tgctgctgga tgtcgaattc ttcgcgcgta accagatcca tacggttgaa cgcgccgccc
    34261 agcatcgcct taatattttt ttccacatct ttggcagggc tgttggcgat ggtttcgctg
    34321 attttcgagc cgacttcctc aaaaagctgc ttgccgaaca taatctgtat ccttcctgaa
    34381 catatcaaat tcaatcggct attgtataag gaaaaatgcc gtctgaaaac gggcggcgga
    34441 taatcggcaa aacataccgc gcctcctttg cggttgctaa attacacctc aaaacacctg
    34501 ccgcctgaaa acggatttca tattccgcgc aacgcctgat tacaagacac tacaaaacaa
    34561 tatgctgttt taaatgattt ttccgacgcg catcgtttca aactcggctt tttaagccat
    34621 taagtgcttt gcaaacaaca gaaattgggt tatgctgaaa cggattattt acaatttcat
    34681 atagttttat tacatatctt attgtgattg aagataattt atccgaatcc ccctttcggg
    34741 tatccggatt ttccgttgta cttttattag aaaaacattt caggcgcaag ttgcttgcaa
    34801 tttcaaagcc tgtaattctg aagttttaga cgaggaatag cacatgaatc ccatgtacat
    34861 tacttttgca atctatctgg ttgcagtcct tctcatcggg cttgccgcct atttttctac
    34921 gcgcaatttc gacgattata ttttgggcgg gcgcagccta ggcccgtttg ttaccgcgat
    34981 gtcggcaggc gcgtccgata tgtccggctg gctgctgatg ggtctgccgg gcgcgattta
    35041 cctgagcggt ttgaatgagg cttggattgc catcggcctc ttggtcggcg cgtatttcaa
    35101 ctggctcttg gtggcgggcc gcctgcgcgt gcataccgaa tacgccaaca atgcgctgac
    35161 gctgccggat tatttcttcc accgcttcgg cgcgggcgga cacttgatga aagtggtttc
    35221 cgcactgatt atcctgttct tcttcacgat ttattgcgcc tcgggcattg tggcgggcgc
    35281 aaccctgttc caaagcctgt ttgaaggtat gacttacaat caggcaatgt ggctgggcgc
    35341 gggcgcgacc atcgcctata ccttcttggg cggcttcttg gcggtcagct ggacggatac
    35401 gctgcaggct tctttaatga ttttcgccct gattttaacg ccggtgatgg tctatctggg
    35461 cttgggcggc gcggaacaga tgtctgccgc gattcaaagc gtcgccgcag gcacgggcaa
    35521 agcatacggc agcctgtttg ccggtacgac cgtcatcggc atcatttcca ccgccgcatg
    35581 gggcttgggc tatttcggac agccgcacat tttggcgcgc tttatggcgg ccgaaagcgc
    35641 gaaatcgctg gtatccgcac gccgcatcgg catgacttgg atggcgttgt gccttgcggg
    35701 cgcggtggcg gtcggttatt tcggcattgc gtatttcggt gcaaatcccg acaaagtttc
    35761 ttctatgagc ggcaaccacg aacgcatctt catcgcgctt tccaccctgc tgttcaaccc
    35821 ttggatcgcc ggcatcattt tgagcgcgat tttggcggcg gtgatgtcca ccctgtcctg
    35881 ccagcttttg gtttgctcca gcgcgattac cgaagacttc tacaaaggct ttttgcgtaa
    35941 aaacgcgcaa cagtcggaac tggtatgggt cggccgcctg atggtcttgg cgattgccgt
    36001 gatttccatc ctgattgctt ccgatccgaa cagcaaagta ttgggactgg tgtcttatgc
    36061 gtgggcaggt ttcggtgcgg cgttcggccc gattgtgatt ctgtccgtat tgtggaaacg
    36121 catcactgcc tacggcgcgt tgtcgggtat ggttgcaggt gccttaaccg tagtcgtatg
    36181 ggcggaatgg ataaaaaaac cggctcaagc agcaggcgaa agcggcttgt tgacgatgta
    36241 cgaaatcgtc ccgggcttta ttgtttgctt aatcattgcc gtgttggttt ccctgttcaa
    36301 caaagaacct tcgcgcgaaa tccaagaacg ctttgaaaaa gccgacgcag attaccgcgc
    36361 cgcccgataa acccctttca gacggcacgg catcccggtt cgtgccgtct gaacccgttt
    36421 ccgggtcaaa cacaaataaa ccttgcctgc atacaggcct tatttgtgtt ttacccgttt
    36481 cattttcaaa gaaacggaag cacctaccct gcccgcattt gaagccttgt atttcatctg
    36541 cggatttatt acgatattac cgtattcagg ccgcaccgat gccgcctgcc ccccgaaaaa
    36601 ctttggagaa tccaaaaaat gtttcatttt gcatttccgg cacaaactgc cctgcgccaa
    36661 gcgataaccg atgcctaccg ccgtaatgaa atcgaagccg tacaggatat gttgcaacgt
    36721 gcacagatga gcgacgaaga gcgcaacgcc gcctccgagc ttgcccgccg tttggttacc
    36781 caagtccgcg ccggccgcac caaagccggc ggcgtggatg cgctgatgca cgagttttca
    36841 ctctccagcg aagaaggcat cgcgctgatg tgtctggcag aagccctgct gcgtatcccc
    36901 gacaacgcca cgcgcgaccg cctgattgcc gacaagattt cagacggcaa ctggaaaagc
    36961 catttgaaca acagcccttc cctcttcgtc aatgctgccg cctggggcct gctgattacc
    37021 ggcaaactga ccgccacaaa cgacaaacaa atgagttccg cactcagccg cctgatcagc
    37081 aaaggcggcg caccgctcat ccgccaaggc gtaaattacg ccatgcggct tctgggcaaa
    37141 cagttcgtaa ccggacagac cattgaagaa gccctgcaaa acggcaaaga acgcgaaaaa
    37201 atgggctacc gcttctcctt cgatatgttg ggcgaagccg cctacaccca agccgatgcc
    37261 gaccgctact accgcgacta tgtcgaagcc atccacgcca tcggcaaaga tgcggcagga
    37321 caaggcgttt acgaaggcaa cggcatttcc gtcaaactct ccgccataca tccgcgctac
    37381 tcgcgcgccc aacacggccg tgtgatgggc gaactgttgc cgcgcctgaa agagctgttc
    37441 cttttgggta aaaaatacga tatcggtatc aacatcgatg ccgaagaagc caaccgtctg
    37501 gagctgtctt tggatttgat ggaggcactc gtttccgatc ccgatttggc aggctacaaa
    37561 ggcatcggtt tcgttgttca agcctaccaa aaacgttgtc cgttcgttat cgactacctg
    37621 atcgaccttg cccgccgcaa caaccaaaaa ctaatgatcc gcctcgtcaa aggcgcgtat
    37681 tgggacagcg aaatcaaatg ggcgcaagtg gacggcttga acggctatcc gacctacacc
    37741 cgcaaagtcc acaccgacat ctcctacctc gcctgcgcgc gcaaactgct ttccgcgcaa
    37801 gacgcggtat ttccgcaatt cgccacccac aacgcctaca ccttgggcgc aatctaccaa
    37861 atgggcaaag gcaaagactt tgaacaccaa tgtctgcacg gcatgggcga aaccctgtac
    37921 gaccaagtcg tcggcccgca aaacttaggc cgccgcgtgc gcgtgtacgc cccggtcggc
    37981 acacacgaaa ccctgctcgc ctacttggtg cgccgcctgt tggaaaacgg cgcgaactcg
    38041 tctttcgtca accaaatcgt cgatgaaaac atcagcatcg acacgctcat ccgcagcccg
    38101 ttcgacacca tcgccgaaca aggcatccac ctgcacaacg ccctgccgct gccgcgcgat
    38161 ttgtacggca aatgccgtct gaactcgcaa ggcgtggact tgagcaacga aaacgtattg
    38221 cagcagcttc aagaacagat gaacaaagcc gccgcgcaag acttccacgc cgcatccatc
    38281 gtcaacggca aagcccgcga tgtcggcgaa gcgcaaccga ttaaaaaccc tgccgaccac
    38341 gacgacatcg tcggcacagt cagctttgcc gatgccgcgc ttgcccaaga agcggttggc
    38401 gcagccgttg ccgcgttccc cgaatggagt gcgacacctg ccgccgaacg cgccgcctgc
    38461 ctgcgccgtt tcgccgacct gctcgaacag cacacccccg cgctgatgat gcttgccgtg
    38521 cgcgaagcag gcaaaacgct gaacaacgcc attgccgaag tgcgcgaagc cgtcgatttc
    38581 tgccgctact acgcaaacga agccgaacat accctgcctc aagacgcaaa agccgtcggc
    38641 gcgattgtcg ccatcagccc gtggaacttc ccgctcgcca tctttaccgg cgaagtcgtt
    38701 tccgcattgg cggcaggcaa caccgtcatc gccaaacccg ccgaacaaac cagcctgatt
    38761 gccggttatg ccgtttccct catgcacgaa gccggcatcc cgacttccgc cctgcaactc
    38821 gtcctcggcg caggcgacgt gggtgcggca ttgaccaacg atgcccgcat cggcggcgtg
    38881 attttcaccg gctcgaccga agtggcgcgc ctgatcaaca aagcccttgc caaacgcggc
    38941 gacaatcccg tcctgattgc cgaaaccggc ggacaaaacg ccatgattgt cgattccacc
    39001 gcacttgccg aacaagtctg cgccgacgta ttgaactccg ccttcgacag cgcgggacaa
    39061 cgctgctccg ccctgcgtat cttgtgcgtc caagaagacg ttgccgaccg tatgctcgac
    39121 atgatcaaag gcgcgatgga cgaactcgtc gtcggcaaac cgattcagct cactaccgat
    39181 gtcggccccg tcatcgatgc cgaagcacag caaaacctgt tgaaccacat caacaaaatg
    39241 aaaggcgttg ccaagtctta ccacgaagtc aaaaccgctg ccgatgtcga ttccgaaaaa
    39301 tccacgttcg tccgccccat cctgtttgaa ttgaacaacc tcaacgaact gcaacgcgaa
    39361 gtcttcggtc ccgtcctgca cgtcgtccgc taccgcgccg acaaactcga cagcgtcatc
    39421 gaccaaatca acagcaaagg ctacgccctg acccacggcg tacacagccg catcgaaggc
    39481 acagtacgcc acatccgcag ccgcatcgaa gccggcaacg tttacgtcaa ccgcaacatc
    39541 gtcggcgcag tcgtcggcgt acagcccttc ggcggacacg gtctgtccgg cacaggcccc
    39601 aaagcaggcg gttcgttcta cctgcaaaaa ctgacccgca tccccgaatg ggttgccccg
    39661 accctgagcc aaatcggaca ggcagacgaa gccgcgctca aacgcctcga agcactggtt
    39721 cacaaactgc cgttcaacgc cgaagagaaa aaagccgcag cggccgcttt gggacacgcc
    39781 cgcatccgca ccctgcgccg tgccgaaacc gtccttaccg gcccgaccgg cgagcgcaac
    39841 agcatctcat ggcacgcgcc aaaacgcgtc tgggtacacg gcggcagcct cctgcaagcc
    39901 ttctgcgccc taaccgaact tgccgcctcc ggcgtacagg cagtggtcga acccgacagc
    39961 ccgctggctt cctacaccgc cgacttggaa ggtctgctgc tggtcaacgg caaacccgaa
    40021 accgccgaca tcagccacgt tgccgccctg tcgcctttgg acagcgcgcg caaacaggaa
    40081 cttgccgccc acgacggcgc actcatccgc atcctccctt cggaaaacgg actcgacatc
    40141 ctgcaagtgt ttgaagaaat ctcttgcagc gtcaacacca cagccgccgg cggcaacgcc
    40201 agcctgatgg cggttgccga ctgattttac cgaaatcccc gagcgcggcc cgtgaaccaa
    40261 tgccgtctga aaacctttca gacggc
//
LOCUS       DAWKOG010000003        38977 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.003, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000003 DAWKOG010000000
VERSION     DAWKOG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 38977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38977
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.003"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            17..328
                     /gene="rpsJ"
                     /locus_tag="WJK66_000091"
     CDS             17..328
                     /gene="rpsJ"
                     /locus_tag="WJK66_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEZ7577869.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHVNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            complement(414..600)
                     /locus_tag="WJK66_000092"
                     /pseudo
     CDS             complement(414..600)
                     /locus_tag="WJK66_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            620..1672
                     /locus_tag="WJK66_000093"
     CDS             620..1672
                     /locus_tag="WJK66_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HEZ7577870.1"
                     /translation="MFYSQVNKGKMMSLLNVFKDILNNKELNSEERHDLERAVKILQD
                     THVNILITGATGCGKSSTINALFSIDQATGKSDGLVKEVAKVGVGVDPETMDIAKYEL
                     ENMTIWDSPGLGDGEKADQKHRRNIINKLLEKDSNGNAVIDLVLVLLDGGSRDLGTSY
                     ELINNVIINTLKKSGEGRTDRLLVAINQCDMAMKGRNWDAEAGRPNAALENFLEEKVC
                     STRKRIQEATGVTVNPIYFSAGFKSEDEEQRPYNITRLLRYIITAMPSEKRVIVAEKI
                     NVEEIERDTSRRKQEERQEIDSSIGSALEKILGAAGGVVKDVVSTIASGVGTLIGGAA
                     KAIGGAISTVASFFGW"
     gene            1681..2364
                     /locus_tag="WJK66_000094"
     CDS             1681..2364
                     /locus_tag="WJK66_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HEZ7577871.1"
                     /translation="MHRYRISEIGQKVTAAGFRHLDVLLVGATGVGKSSTLNALFGEQ
                     KAKVGTGVDPETQLVESYMLNDVLRFWDSAGLGDGKEADRAHRQKLIDVLSKTYTHSD
                     GQWGWIDLVFVILDGSSRDLGTAYDLLRDVILKMIDPDRVIVAINQADMAMKGRYWDK
                     VLHQPQPNLQQFLDEKAESVQKRILEATGLQISKPVYYSAYENYHLKEIMDAVINHIP
                     VCRRKMHTP"
     gene            2583..3227
                     /gene="rplC"
                     /locus_tag="WJK66_000095"
     CDS             2583..3227
                     /gene="rplC"
                     /locus_tag="WJK66_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEZ7577872.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            3227..3847
                     /gene="rplD"
                     /locus_tag="WJK66_000096"
     CDS             3227..3847
                     /gene="rplD"
                     /locus_tag="WJK66_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEZ7577873.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            3844..4158
                     /gene="rplW"
                     /locus_tag="WJK66_000097"
     CDS             3844..4158
                     /gene="rplW"
                     /locus_tag="WJK66_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEZ7577874.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            4164..4997
                     /gene="rplB"
                     /locus_tag="WJK66_000098"
     CDS             4164..4997
                     /gene="rplB"
                     /locus_tag="WJK66_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEZ7577875.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVNCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            5004..5282
                     /gene="rpsS"
                     /locus_tag="WJK66_000099"
     CDS             5004..5282
                     /gene="rpsS"
                     /locus_tag="WJK66_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEZ7577876.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            5291..5620
                     /gene="rplV"
                     /locus_tag="WJK66_000100"
     CDS             5291..5620
                     /gene="rplV"
                     /locus_tag="WJK66_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEZ7577877.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            5630..6322
                     /gene="rpsC"
                     /locus_tag="WJK66_000101"
     CDS             5630..6322
                     /gene="rpsC"
                     /locus_tag="WJK66_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEZ7577878.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            6306..6722
                     /gene="rplP"
                     /locus_tag="WJK66_000102"
     CDS             6306..6722
                     /gene="rplP"
                     /locus_tag="WJK66_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEZ7577879.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            6722..6913
                     /gene="rpmC"
                     /locus_tag="WJK66_000103"
     CDS             6722..6913
                     /gene="rpmC"
                     /locus_tag="WJK66_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEZ7577880.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            6913..7176
                     /gene="rpsQ"
                     /locus_tag="WJK66_000104"
     CDS             6913..7176
                     /gene="rpsQ"
                     /locus_tag="WJK66_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEZ7577881.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            7398..7766
                     /gene="rplN"
                     /locus_tag="WJK66_000105"
     CDS             7398..7766
                     /gene="rplN"
                     /locus_tag="WJK66_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEZ7577882.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            7778..8101
                     /gene="rplX"
                     /locus_tag="WJK66_000106"
     CDS             7778..8101
                     /gene="rplX"
                     /locus_tag="WJK66_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEZ7577883.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            8111..8650
                     /gene="rplE"
                     /locus_tag="WJK66_000107"
     CDS             8111..8650
                     /gene="rplE"
                     /locus_tag="WJK66_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEZ7577884.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAI
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            8653..8958
                     /gene="rpsN"
                     /locus_tag="WJK66_000108"
     CDS             8653..8958
                     /gene="rpsN"
                     /locus_tag="WJK66_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEZ7577885.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            8974..9366
                     /gene="rpsH"
                     /locus_tag="WJK66_000109"
     CDS             8974..9366
                     /gene="rpsH"
                     /locus_tag="WJK66_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEZ7577886.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            9380..9913
                     /gene="rplF"
                     /locus_tag="WJK66_000110"
     CDS             9380..9913
                     /gene="rplF"
                     /locus_tag="WJK66_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEZ7577887.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLAFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            9927..10280
                     /gene="rplR"
                     /locus_tag="WJK66_000111"
     CDS             9927..10280
                     /gene="rplR"
                     /locus_tag="WJK66_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEZ7577888.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            10299..10817
                     /gene="rpsE"
                     /locus_tag="WJK66_000112"
     CDS             10299..10817
                     /gene="rpsE"
                     /locus_tag="WJK66_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEZ7577889.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            10810..10995
                     /gene="rpmD"
                     /locus_tag="WJK66_000113"
     CDS             10810..10995
                     /gene="rpmD"
                     /locus_tag="WJK66_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEZ7577890.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            10997..11431
                     /gene="rplO"
                     /locus_tag="WJK66_000114"
     CDS             10997..11431
                     /gene="rplO"
                     /locus_tag="WJK66_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEZ7577891.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIAFGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            11443..12753
                     /gene="secY"
                     /locus_tag="WJK66_000115"
     CDS             11443..12753
                     /gene="secY"
                     /locus_tag="WJK66_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEZ7577892.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            12758..12976
                     /gene="infA"
                     /locus_tag="WJK66_000116"
     CDS             12758..12976
                     /gene="infA"
                     /locus_tag="WJK66_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEZ7577893.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            12997..13110
                     /gene="rpmJ"
                     /locus_tag="WJK66_000117"
     CDS             12997..13110
                     /gene="rpmJ"
                     /locus_tag="WJK66_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEZ7577894.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            13176..13538
                     /gene="rpsM"
                     /locus_tag="WJK66_000118"
     CDS             13176..13538
                     /gene="rpsM"
                     /locus_tag="WJK66_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEZ7577895.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            13558..13953
                     /gene="rpsK"
                     /locus_tag="WJK66_000119"
     CDS             13558..13953
                     /gene="rpsK"
                     /locus_tag="WJK66_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEZ7577896.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            13973..14593
                     /gene="rpsD"
                     /locus_tag="WJK66_000120"
     CDS             13973..14593
                     /gene="rpsD"
                     /locus_tag="WJK66_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEZ7577897.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            14619..15605
                     /gene="rpoA"
                     /locus_tag="WJK66_000121"
     CDS             14619..15605
                     /gene="rpoA"
                     /locus_tag="WJK66_000121"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEZ7577898.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPSHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            15629..15997
                     /gene="rplQ"
                     /locus_tag="WJK66_000122"
     CDS             15629..15997
                     /gene="rplQ"
                     /locus_tag="WJK66_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEZ7577899.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHEAIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            16144..16857
                     /gene="minC"
                     /locus_tag="WJK66_000123"
     CDS             16144..16857
                     /gene="minC"
                     /locus_tag="WJK66_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEZ7577900.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQNTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNIHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPNHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            16885..17700
                     /gene="minD"
                     /locus_tag="WJK66_000124"
     CDS             16885..17700
                     /gene="minD"
                     /locus_tag="WJK66_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEZ7577901.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            17704..17967
                     /gene="minE"
                     /locus_tag="WJK66_000125"
     CDS             17704..17967
                     /gene="minE"
                     /locus_tag="WJK66_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEZ7577902.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            17971..18891
                     /gene="oxyR"
                     /locus_tag="WJK66_000126"
     CDS             17971..18891
                     /gene="oxyR"
                     /locus_tag="WJK66_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HEZ7577903.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
     gene            complement(18888..19079)
                     /locus_tag="WJK66_000127"
     CDS             complement(18888..19079)
                     /locus_tag="WJK66_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7577904.1"
                     /translation="MLIHYTWHCIGTDENIHSDAKVEYLQDGSFRGDAILKIDDDGNI
                     LAYRVVGSGKWRFENNACA"
     gene            19465..19854
                     /locus_tag="WJK66_000128"
     CDS             19465..19854
                     /locus_tag="WJK66_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ7577905.1"
                     /translation="MRYTIKTFQTLADETFPSKNFKILSFNGVARPITIVCPTHGVQT
                     VSAGQAFIRSKHGCPACGLVLSTSVLASRGRSVSILDTATGETLSFPSVQAAAKALNT
                     SYGSIRTKLDGRSSPDNLVCNRYKVMP"
     gene            complement(20225..23062)
                     /locus_tag="WJK66_000129"
     CDS             complement(20225..23062)
                     /locus_tag="WJK66_000129"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEZ7577906.1"
                     /translation="MLDKYSPAEIESKHYQNWEEQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLVNVFDLEAKVLANAEVFNFKGEAQPSFALPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDNEG
                     NVYVARNQEEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIDLEKLLVKRTTGLRKPETAPKVEEASRKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATATEPRGYSFPDMWIVDRL
                     NQTIEQVTQAYETYRFDLAAETLYSFMWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPEADGGDIVQTAFEQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESTDDLADYLKYLPMMTRLIEAQQVAAL
                     PESEDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQNQLAKLKD"
     gene            complement(23159..23968)
                     /gene="zupT"
                     /locus_tag="WJK66_000130"
     CDS             complement(23159..23968)
                     /gene="zupT"
                     /locus_tag="WJK66_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HEZ7577907.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTMGMAVIAVSLVLFHF"
     gene            complement(24149..25405)
                     /locus_tag="WJK66_000131"
     CDS             complement(24149..25405)
                     /locus_tag="WJK66_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEZ7577908.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAKGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWRMLQNCTAAHYQ
                     TNKERMVRISEYSREMFRRFEAQTGMNFEGRKKGTLQIFRQAKEVEAAEQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCRLFTENLYKLCQEKGVRF
                     HFNQTISRIDHNGLRIKTVETETGRFEADAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQASFWSGLRPMTPDSTPLIGRTRFDNLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            complement(25484..26875)
                     /locus_tag="WJK66_000132"
     CDS             complement(25484..26875)
                     /locus_tag="WJK66_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ7577909.1"
                     /translation="MQLFLDNLKAFFETVSGWVWGSVMLMLLVGTGIVLTVMLKGLQF
                     TMLGYALKQAFVPSKKYEGGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            complement(27232..28008)
                     /gene="lpxA"
                     /locus_tag="WJK66_000133"
     CDS             complement(27232..28008)
                     /gene="lpxA"
                     /locus_tag="WJK66_000133"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEZ7577910.1"
                     /translation="MTLIHPTAVIDPKAELDSGVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTVGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFRDFFAQSARGIIR"
     gene            complement(28095..28544)
                     /gene="fabZ"
                     /locus_tag="WJK66_000134"
     CDS             complement(28095..28544)
                     /gene="fabZ"
                     /locus_tag="WJK66_000134"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEZ7577911.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAVIMCAKRVV"
     gene            complement(28578..29624)
                     /gene="lpxD"
                     /locus_tag="WJK66_000135"
     CDS             complement(28578..29624)
                     /gene="lpxD"
                     /locus_tag="WJK66_000135"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEZ7577912.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPSATVPASCEIGANAYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDAGQDSK"
     gene            complement(29657..30157)
                     /locus_tag="WJK66_000136"
     CDS             complement(29657..30157)
                     /locus_tag="WJK66_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEZ7577913.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKAQAEEKWRGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(30223..32616)
                     /gene="bamA"
                     /locus_tag="WJK66_000137"
     CDS             complement(30223..32616)
                     /gene="bamA"
                     /locus_tag="WJK66_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEZ7577914.1"
                     /translation="MKLKQIASALMVLGISPLALADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGERFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKKYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEIGYANGYGKTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(32673..34013)
                     /gene="rseP"
                     /locus_tag="WJK66_000138"
     CDS             complement(32673..34013)
                     /gene="rseP"
                     /locus_tag="WJK66_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEZ7577915.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLNYERAGQTHT
                     ADIRPDTVEQSDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVIRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            complement(34048..35283)
                     /locus_tag="WJK66_000139"
     CDS             complement(34048..35283)
                     /locus_tag="WJK66_000139"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEZ7577916.1"
                     /translation="MPSENASDGIRYKVIPIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFQPEYAVVADAEYAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAVFQVLPRDYAGRLNEHGIRSIILTASGGPFLTTDLGTFDRITPA
                     QAVKHPNWRMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGVAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLSQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(35288..36085)
                     /locus_tag="WJK66_000140"
     CDS             complement(35288..36085)
                     /locus_tag="WJK66_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEZ7577917.1"
                     /translation="MLKQRVMTAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMGGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVMGLVWVADICAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSKLLPGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            complement(36088..36834)
                     /locus_tag="WJK66_000141"
     CDS             complement(36088..36834)
                     /locus_tag="WJK66_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEZ7577918.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVRHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKYLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            complement(36890..37447)
                     /gene="frr"
                     /locus_tag="WJK66_000142"
     CDS             complement(36890..37447)
                     /gene="frr"
                     /locus_tag="WJK66_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEZ7577919.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            complement(37628..38755)
                     /locus_tag="WJK66_000143"
     CDS             complement(37628..38755)
                     /locus_tag="WJK66_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEZ7577920.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRYRRLIHAVRLGGAVLFATASARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWFDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLTDCSKMIAEISNGRRMTRERLEQNMVKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            complement(38825..38900)
                     /locus_tag="WJK66_000144"
     tRNA            complement(38825..38900)
                     /locus_tag="WJK66_000144"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38865..38867),aa:Thr,seq:cgt)
BASE COUNT         9310 a         8762 c        10608 g        10297 t
ORIGIN      
        1 tgaaggatat tgataaatgg caaaccaaaa aatccgtatc cgcctgaaag cttatgatta
       61 cgccctgatt gaccgttctg cacaagaaat cgttgaaact gcaaaacgta ccggtgcagt
      121 tgtaaaaggc ccgattcctt tgccgaccaa aatcgaacgt ttcaacattc tgcgttctcc
      181 gcacgtgaac aaaacttccc gtgaacaatt ggaaatccgc acccacttgc gcctgatgga
      241 catcgtggat tggaccgaca aaactaccga tgcgctgatg aagctggatt tgccggccgg
      301 tgttgatgta gaaatcaaag tccaataatt agggctgttg aaaatcccca agcaattaat
      361 gcttggggat tttttatgtt atgccgtctg aaaatatgcg gcggcaaaca gatttaaggc
      421 aaaacaacaa aatcaaaaaa cagtgcggtc ggcgaaatcg gcttattttg tgcctcaaga
      481 attttcagac ggcatttttt agtgaacaaa acaggatatt ttttatccat agccttccga
      541 aggcttgccc cccccccgaa gaaaggtaga atcgggcttg gtttgggcag aagcatccat
      601 ctgattctaa ggatggatga tgttttattc tcaagtaaat aaaggaaaga tgatgagctt
      661 acttaatgtc tttaaagata ttttaaacaa caaagaattg aattcggaag aacggcatga
      721 tttggagcgg gcggttaaga tcttgcagga tacgcatgtc aatattttga ttaccggagc
      781 caccggttgc ggaaaatcat ccaccatcaa tgccttgttc agcatagatc aggcaacggg
      841 aaagagtgat ggactagtaa aggaagtcgc aaaagtaggc gtgggtgtag atcctgaaac
      901 tatggatatt gccaaatatg aattggagaa tatgacgatt tgggatagtc cgggcttggg
      961 cgacggagag aaggcagacc aaaagcaccg gcgcaatatc atcaataagc tgctggagaa
     1021 agacagtaac ggcaatgccg tcatcgactt ggttttggtg ctgcttgacg gcggctccag
     1081 agacttgggg acatcatacg aactgattaa caatgtaatc atcaatacat tgaagaagag
     1141 tggggaaggc cgtacagacc ggttgctggt tgcgattaat cagtgtgata tggcgatgaa
     1201 ggggcggaat tgggatgcgg aagccggccg tccgaacgcc gccttggaaa actttttgga
     1261 ggagaaagtg tgttcgacac gcaagcgcat tcaggaagct accggcgtta cggtcaatcc
     1321 gatttatttt tcagcaggct ttaagagtga ggatgaggag cagcgtccct ataacataac
     1381 ccgcctgttg cgttacatca ttacggctat gccgtctgaa aaacgggtga ttgtggcaga
     1441 gaagattaat gtggaagaaa tcgaacgaga taccagccgc cgcaaacagg aggagcggca
     1501 agaaatcgac agtagtatcg gcagtgcgct ggagaaaata ttgggggctg cgggaggcgt
     1561 ggttaaagat gttgtttcaa cgatagcaag cggtgtcggg acgctgatcg gcggtgctgc
     1621 aaaagcgatt ggaggggcga ttagcacggt tgcttctttt tttggctggt aggagcgcat
     1681 atgcatcgtt accgtatttc tgaaatcggg caaaaagtca cagcagccgg tttccgtcat
     1741 ttggatgtct tgcttgtcgg tgcgacaggt gtcggcaagt cctcaacctt aaatgccttg
     1801 tttggcgaac aaaaagccaa ggtaggcaca ggcgtggatc ccgaaacgca attggtcgaa
     1861 agctatatgc tgaacgatgt gttgcggttt tgggacagcg caggtttggg cgatgggaaa
     1921 gaagccgacc gcgcccatcg gcaaaaactg attgatgtcc tgtctaaaac ctatactcat
     1981 tcggatgggc agtggggctg gatagatttg gtgttcgtta tccttgacgg cagctcccgc
     2041 gatttgggta cggcctatga tttgttgagg gatgttatcc ttaaaatgat tgatccggat
     2101 agggttattg ttgcaatcaa tcaggcggat atggcgatga aaggacgtta ttgggataag
     2161 gtgctgcatc agccgcagcc gaatttgcaa cagtttctag acgagaaggc cgaatcggta
     2221 caaaaaagga ttctggaagc aaccggtttg cagatttcca aacccgttta ctattcggca
     2281 tacgaaaact accatctcaa agaaataatg gatgcagtca tcaaccatat cccggtttgt
     2341 cggagaaaga tgcatacgcc gtgatggtcg gataccgact cctttaaaag catccgaccc
     2401 tatgccgtct gaagattcaa aacgcttcag acggcatatt gaagatattt ttgatatttt
     2461 tgttgatatt tctttgactt gtcagatata atgtctgact tggtacattc gtaccaagtt
     2521 taactttgtc tgaaagacag gccaatcgta gcctgtccct ttactttaaa aggaaaataa
     2581 tcatgacttt aggtctggtt ggacgcaaag ttggtatgac ccgcgtgttc gacgaacagg
     2641 gtgtttctgt tccggtaacc gttttggata tgtctgccaa ccgcgttaca caagtaaaat
     2701 ccaaagatac tgacggctat actgccgttc aagttacctt tggtcagaaa aaagccaatc
     2761 gtgtcaacaa agccgaagcc gggcactttg caaaagcagg tgttgaagcc ggtcgcggtt
     2821 tgattgagtt tgctttgact gaagaaaaac tggctgaatt gaaagctggt gacgaaatca
     2881 ccgtttctat gtttgaagtc ggtcaactgg tcgatgtaac cggtacctct aaaggtaaag
     2941 gtttctccgg cacgatcaaa cgtcataact tcggtgccca acgtacttcc cacggtaact
     3001 cccgttctca ccgtgttcca ggctctatcg gtatggcgca agacccgggt cgcgtgttcc
     3061 ccggtaaacg catggccggc caatacggca acaccaaagc aactgttcaa aaattggaag
     3121 ttgtccgtgt tgatgcagaa cgccaactgc tgttggttaa gggtgctgtt ccgggtgcgg
     3181 tcaacagcga tgttgtagtt cgtcccagcg tgaaagtagg tgcgtaatgg aattgaaagt
     3241 aattgacgct aaaggacaag tttcaggcag cctgtctgtt tctgatgctt tgttcgcccg
     3301 cgaatacaat gaagcgttgg ttcatcagct ggtaaatgcc tacttggcaa acgcccgctc
     3361 tggtaaccgt gctcaaaaaa cccgtgccga agtaaaacac tcaaccaaaa aaccatggcg
     3421 tcaaaaaggt accggccgcg cccgttccgg tatgacttca tctccgctgt ggcgtaaagg
     3481 tggtcgcgcg ttcccgaaca aacccgacga aaacttcact caaaaagtaa accgcaaaat
     3541 gtaccgtgcc ggtatggcga ctattctgtc ccaattgact cgtgacgagc gtttgtttgc
     3601 gattgaggcg ttgactgctg aaactcctaa aaccaaagtt tttgctgaac aagtgaaaaa
     3661 tctgggtctg gagcaagtgt tgtttgtaac caaacagctc gacgagaatg tttacttggc
     3721 ttcacgcaac ttgccaaacg tgttggtttt ggaagctcaa caagttgatc cttacagctt
     3781 gctgcgttac aaaaaagtaa tcatcactaa agatgcagtt gcacaattag aggagcaatg
     3841 ggtatgaatc aacaacgttt gactcaagtg attttggcac ctatcgtttc tgaaaaaagc
     3901 aacgtattgg ctgaaaaacg caaccaaatg acgtttaaag tcttggcaaa tgcaaccaaa
     3961 cctgaaatca aagctgctgt tgagctgctg tttggtgttc aagttgcttc tgtaactacc
     4021 gttacaacta aaggcaaaac taagcgtttt ggtcgtactt tgggccgccg cagcgatgtt
     4081 aaaaaagctt atgtaagctt ggctgccggt caagagttgg atttggaagc cgctgctgca
     4141 gctgcagata aggaataaac aaaatggcaa tcgttaaaat gaagccgacc tctgcaggcc
     4201 gtcgcggcat ggttcgcgtg gtaacagaag gtttgcacaa aggtgcacct tatgcgccct
     4261 tgcttgaaaa gaaaaattct actgccggcc gtaacaataa tggtcatatc accacccgtc
     4321 acaaaggcgg cggtcataaa caccattacc gtgttgtaga ctttaaacgt aacaaagacg
     4381 gcattcctgc caaagtagag cgtatcgaat acgatcctaa ccgtactgcc ttcatcgcac
     4441 tgttgtgcta tgcagacggc gagcgtcgtt acatcattgc tcctcgcggt attcaagccg
     4501 gtgctgtatt ggtttccggt gctgaagctg ccatcaaagt aggcaacacc ctgccgattc
     4561 gcaacattcc cgttggtacg actatccact gtatcgaaat gaaacccggt aaaggtgctc
     4621 aaatcgcacg ttctgccggt gcttctgcgg tattgctggc taaagaaggc gcgtacgctc
     4681 aagtccgtct gcgctctggc gaagtccgta aaatcaacgt aaattgccgt gcgaccattg
     4741 gtgaagttgg taacgaagag caaagcctga aaaaaatcgg taaagccggt gccaaccgtt
     4801 ggcgcggtat tcgtccgacc gtacgcggtg ttgtcatgaa tcctgtcgat cacccgcatg
     4861 gtggtggtga aggccgtacg ggcgaggccc gcgaaccggt cagcccatgg ggtactcctg
     4921 ctaaaggcta ccgcactcgt aataacaaac gcacggataa catgattgtt cgtcgccgtt
     4981 actcaaataa aggttaattt agtatggctc gttcattgaa aaaaggccca tatgtagacc
     5041 tgcatttgct gaaaaaagta gatgctgctc gcgcaagcaa cgacaaacgc ccgattaaaa
     5101 cctggtctcg tcgttctacc attctgcctg attttatcgg tctgaccatt gctgtgcaca
     5161 acggccgcac ccatgtgcct gtgtttatca gcgacaatat ggttggtcat aaattaggcg
     5221 aattctcatt gacccgtacc tttaaaggcc acttggccga taaaaaggct aaaaagaaat
     5281 aaggtgaatc atgagagtaa atgcacaaca taaaaatgcc cgtatctctg ctcaaaaggc
     5341 tcgtttggta gctgatttga ttcgtggtaa agacgttgcc caagctttga atattttggc
     5401 tttcagtcct aaaaaaggtg ccgagctgat taaaaaagta ttggagtcag ctattgctaa
     5461 tgccgagcac aataacggtg cggacattga tgaactgaaa gtggtaacta tctttgttga
     5521 caaaggccca agcttgaaac gttttcaagc tcgcgccaaa ggtcgcggta accgcatcga
     5581 aaaacaaact tgtcatatca atgtgacagt gggtaactaa ggaaaagcta tgggacaaaa
     5641 gattaaccct acaggctttc gcctggcggt aactaaagac tgggcttcaa aatggtttgc
     5701 taaaagcacc gacttttcta ctgttttgaa gcaggatatc gatgttcgca attatttgcg
     5761 tcaaaaattg gccaatgctt cggttggtcg agtggttatt gaacgccctg caaaatctgc
     5821 acgcattacc attcactccg ctcgtccggg tgtggttata ggtaaaaaag gtgaggatat
     5881 cgaggttttg aaacgtgact tgcaagtctt gatgggtgta cctgttcatg taaatattga
     5941 agagattcgc cgtcctgagt tggatgctca aattattgct gacggtattg cccagcagtt
     6001 ggaaaagcgc gttcaattcc gtcgtgctat gaaacgagca atgcaaaatg caatgcgttc
     6061 tggtgctaaa ggcattaaga ttatgacttc aggccgtctg aatggtgcgg atattgcccg
     6121 tagcgaatgg tatcgtgaag gtcgcgtgcc actgcatact ttacgtgcaa atgtagatta
     6181 tgcaaccagc gaagcgcaca ccacatatgg tgtattgggt ctgaaagttt gggtttatac
     6241 ggaaggcaat attaaatctt ccaaacctga acatgagagt aaacaaagaa aggcaggtag
     6301 acgtaatgct gcagccaact agactgaaat accgtaagca acaaaagggt cgcaatactg
     6361 gcatcgctac tcgcggtaat aaggtaagtt tcggtgagtt cggcttgaaa gccgtaggtc
     6421 gtggtcgttt gactgcccgt caaatcgaag ctgctcgtcg tgcaatgacc cgtcatatca
     6481 aacgtggtgg tcgtatttgg attcgtgtat tccctgataa accgattact gaaaagccta
     6541 ttcaagttcg tatgggtggc ggtaaaggta acgtggaata ttacattgcc gaaattaaac
     6601 caggtaaagt gttgtatgaa atggatggcg ttccagagga actggctcgt gaagcattcg
     6661 agttggctgc tgccaaattg cctattccta caacctttgt agtaagacag gtgggtcaat
     6721 aatgaaagca aatgaattga aagacaaatc cgttgagcag ttgaatgcag atttgttgga
     6781 cttgttgaaa gctcagtttg gcttacgtat gcaaaacgct accggtcaat taggcaaacc
     6841 aagtgaattg aaacgtgtac gtcgcgatat tgctcgtatt aaaaccgttt taactgaaaa
     6901 aggtgctaag taatgagcga aactaaaaat gttcgtactt tgcaaggcaa agtagtaagc
     6961 gacaaaatgg ataaaaccgt aacagtattg gttgagcgta aagtaaaaca tccgctgtat
     7021 ggtaagatta ttcggttatc tactaaaatc catgcccatg atgaaaataa tcaatatgga
     7081 attggtgatg tggttgttat atcggaatcc cgtccattgt caaaaactaa atcttgggtt
     7141 gtcagtgagc tggttgagaa agcacgttct atttaagaat taaagcaacg tgcttggaat
     7201 gggaaacgaa gtattgcagc aaatttaatt tgcgtgtaaa cttcgtttcc tgtctttcag
     7261 tttcttctgg aagtttcttc cctttcgggg tccaagactg gtttacttga accgcaaggt
     7321 ttcatttaat aagcagcggc tttgctgtaa gttatctgaa agtggtaaat taagttggtt
     7381 aatttaaagg taataacatg attcaaatgc agaccatctt agatgtggct gataactctg
     7441 gtgcgcgtcg cgtaatgtgt atcaaggtat tgggcggatc taagcgtcgc tacgcttctg
     7501 ttggcgatat tattaaagtg gcagttaaag atgcggctcc gcgtggccgt gtcaaaaaag
     7561 gcgatgtata taatgcggta gttgttcgta ctgctaaggg tgtacgtcgt cctgatggtg
     7621 cgttaattaa attcgataac aatgccgccg tgttactgaa taataaactt gaacctttgg
     7681 gtactcgtat ctttggtccg gtaacccgtg aattgcgtac tgagcgattt atgaaaatcg
     7741 tttcattggc acctgaagta ttataaggaa tggcacgatg aataaaatca ttaaaggcga
     7801 tagggttgta gtaattgctg gtaaggataa aggtaagcag ggtcaagtag ttcgagtgtt
     7861 gggtgataaa gttgttgttg agggcgttaa tgttgtaaaa cgccatcaaa aacctaatcc
     7921 aatgcgtggc attgagggcg gtattattac taaagaaatg cctttggata tttctaatat
     7981 cgcaatcctg aatccggaaa ctaataaagc ggaccgtgtt ggtattaagc tgattgaaaa
     8041 tgaaggcaaa gttaaacgcg ttcgtttctt caaatcaaat ggctctatca ttggggcata
     8101 aggagataac atggctcggt tgagagagtt ttataaagag acagttgttc ctgaattggt
     8161 taaacaattt ggttacaaat cagtaatgga agtcccgcgt attgaaaaaa tcaccttgaa
     8221 tatgggtgtg ggtgaggcta ttgctgataa aaaagttatg gaacatgctg tttccgattt
     8281 agagaaaatt gccggtcaaa aaccggttgt tactgttgcc cgtaaatcta tcgcaggttt
     8341 taaaatccgt gataactatc cggttggttg caaagtaaca ttgcgtcgtg atcaaatgtt
     8401 tgaattcttg gatcgtttga ttactattgc attacctcgc gtacgtgact tccgtggtgt
     8461 gagcggtaaa tcatttgatg gccgtggcaa ttacaatatg ggtgttcgtg agcaaattat
     8521 ttttccggaa attgaatacg ataaaattga tgctttgcgt ggtttgaata ttactattac
     8581 tactacagca aaaaccgatg aggaagcgaa agctttattg tcattgttta aatttccgtt
     8641 caaaggataa tcatggctaa gaaagcactt attaatcgtg atctgaaacg tcaagctttg
     8701 gctaaaaaat atgcggctaa acgcgcggca attaaagcgg taatcaatga ttcgaatgca
     8761 actgaggaag agcgttttga ggctcgtttg aggtttcaat ccattcctcg taatgcggca
     8821 cctgtgcgtc aacgtcgtcg ttgtgctttg acaggtcgcc ctcgtggtac tttccgtaaa
     8881 tttggtttgg gtcgtattaa aatccgtgaa atcgccatgc gtggcgaaat tccgggtgtt
     8941 gttaaagcca gctggtaata ggagtaatta agaatgagta tgcatgatcc tatttccgat
     9001 atgttgactc gtatccgcaa tgcgcaacgt gctaataaag cagcggttgc aatgccttct
     9061 tcaaaattaa agtgtgctat tgcaaaggta ttgaaagaag aaggatatat tgaggacttc
     9121 gcagtttcat ctgacgtaaa gtctatattg gaaattcaat taaaatacta tgcaggtcgt
     9181 cctgtaattg aacaaatcaa gcgtgtatct cgccccggtt tgcgtattta taaagcgtct
     9241 agtgagattc caagtgttat gaatggcttg ggtattgcta ttgttagtac ttctaaaggt
     9301 gtaatgactg atcgtaaagc acgttctcaa ggtgttggtg gtgagttgtt atgcattgta
     9361 gcctagtgga ggaaaagaaa tgtcacgtgt cgcaaaaaac ccagtgactg ttcccgctgg
     9421 tgtagaagta aaatttggaa cagaggcatt agttattaag ggtaagaacg gtgaattgtc
     9481 ttttcctttg cattctgatg tagccattga atttaatgat ggcaaattgg cttttgttgc
     9541 gaataacagc agtaaacaag caaatgcaat gtctggtact gctcgcgcat tagtcagcaa
     9601 tatggttaaa ggtgtttcag aaggttttga gaaaagattg caattgatag gtgtgggtta
     9661 tcgtgctcaa gcacaaggta aaatcttgaa tctgtctttg ggtttttctc atccgatcgt
     9721 atatgaaatg cctgaaggtg tctccgttca aactcctagc caaacagaga ttgttttaac
     9781 cggctcggat aaacaagttg ttggtcaagt tgctgctgag attcgtgcgt tccgtgctcc
     9841 tgagccttat aaaggtaaag gtgttcgcta tgtaggcgaa gtagtggtaa tgaaagaagc
     9901 caagaaaaaa taattgaggt tcactaatgg ataaacatac aacccgactc cgtcgtgcac
     9961 gcaaaacccg tgctcgtatt gcggacttga aaatggtaag attatgtgtg ttccgaagca
    10021 ataatcatat ttatgctcaa gtaattagtg ctgaaggtga taaagtattg gctcaagcct
    10081 ctacattgga agctgaggtg cgcggtagtc tgaaatctgg aagcaatgtt gaagcagctg
    10141 caatagttgg taaacgtatc gctgagaagg ctaaagcagc aggtgtagaa aaggttgctt
    10201 ttgatcgttc aggtttccaa tatcacggtc gtgtgaaggc tttggctgaa gctgctcgtg
    10261 aaaatggttt aagcttctaa atatttggag actttcagat ggcaaaacat gaaattgaag
    10321 aacgcggtga cggtctgatt gaaaagatgg tcgcagttaa ccgtgtaacc aaagtagtta
    10381 aaggtggtcg cattatggct ttctctgcgc taactgttgt tggtgatgga gatggtcgta
    10441 ttggtatggg taaaggtaag tcaaaagaag tgcctgttgc agtccaaaag gcgatggatc
    10501 aagcacgacg ctctatgatt aaggtaccat taaaaaatgg tacgattcat catgaggtta
    10561 ttggtcgaca tggtgctact aaagtattta tgcagcctgc taaagagggt agtggcgtaa
    10621 aagccggtgg acctatgcgt ttggtttttg atgctatggg cattcataat atctccgcca
    10681 aagtgcacgg atctactaac ccatataata tcgtacgtgc aacattagat ggtttgtcta
    10741 agttgcatac tcctgctgat atcgcagcca aacgtggctt gacagtggaa gacattttgg
    10801 gagttaacca tggctgaaca aaaaaagatt agggttacat tggttaaaag cctgattggt
    10861 acaattgaat ctcatcgtgc atgtgcacgc ggtttaggtt tgcgtcgtcg cgagcatacg
    10921 gtagaggttt tagatacccc tgaaaaccgt ggtatgatta ataaaatcag ctacttgttg
    10981 aaagtggagt cttgatatgt ttttgaatac aattcaacct gctgttggtg ctacgcatgc
    11041 tggtcgtcgt gttggacgcg gtattggtag tggtcttggc aaaacgggtg gtcgtggtca
    11101 taaaggtcaa aagagccggt ctggtgggtt tcataaggtg ggtttcgaag gtggtcaaat
    11161 gcccttgcaa cgacgcctcc ctaaaagagg ttttaaatct ttaacagcat cagctaatgc
    11221 acagcttcgt ttaagtgaac tggaatcaat tgctgttaat gagattgata ttttggtctt
    11281 aaagcaagcg ggtctgattg catctacagt ctctaatgtt aaagttattg cttttggtga
    11341 aatttctaag gcagttgctt tgaagggtat taaagttacc aaaggtgcga gagctgctat
    11401 cgaggctgtt ggtggtaaga ttgaaatgta aggtttaata ttgtggctaa tcaacaaacg
    11461 tcatcaggtt catccaaatt tggagatctt aagaaacgtc ttttgtttct atttggagca
    11521 ttgattgttt ttcgaattgg tgcccatata cccgtacctg gagttgatgc tgttgcttta
    11581 gctaaattat acgaaagcgc tggaaacggc atcctgggaa tattgaatat gttttccggt
    11641 gggtcgttag agcgctttag tatatttgca ataggaatta tgccatatat ttcagcttct
    11701 attattgtac agctcgcttc tgaaattttg ccatcattga aggctttaaa aaaagaaggg
    11761 gaggctggta gaaaggtaat tacgaaatat actaggtatg gtactgtttt gttagcaatt
    11821 cttcaaagtc taggtgttgc atctttcgta tttcagcaag gaattgttgt aacaagttca
    11881 tttgagtttc atgtttccac ggtagtttct ttggtaacgg gaactatgtt tcttatgtgg
    11941 cttggggagc aaattactga aaggggtatc gggaacggta tttctttaat cattacggca
    12001 ggtattgctt caggtattcc ttcgggtatt gcaaagctgg ttacactgac gaaccaaggt
    12061 tctatgagca tgcttacggc gttgtttatt gtatttggtg ccttattatt aatttatttg
    12121 gttgtatact ttgaaagtgc acagcggaag attcctattc attatgcaaa acgccagttt
    12181 aatggtaggg cgggtagtca aaatacgcat atgcctttca agttgaatat ggctggtgtt
    12241 attcccccaa tttttgcttc cagtattatt ctatttccat ctactctttt aggttggttt
    12301 ggttcggctg atacaaatag tgttttgcac aaaatagctg gattgttaca acacggtcaa
    12361 ttgctgtata tggctttatt tgcagcgaca gttattttct tttgttattt ttatacggct
    12421 ttggttttta gccctaaaga aatggcagag aatttaaaaa agagtggtgc ttttgttcct
    12481 gggattagac ctggtgagca gacctctagg tatttagaaa aagttgtatt acgtttgaca
    12541 ttgtttggag ctctttatat tacaactatt tgtttaattc cagagttctt aactacggtt
    12601 ttaaatgtac ctttttattt gggtggcacg tctttgttga ttctagttgt tgtaacgatg
    12661 gattttagta cacaaataaa ttcgtatagg cttactcaac agtatgataa gttaatgact
    12721 cgttcagaaa tgaaatcatt ttctcggaaa tagaattatg gcgaaagaag atactatcca
    12781 aatgcaaggt gaaattcttg aaactttacc taatgcaaca tttaaagtaa aacttgagaa
    12841 tgaccatatt gtattgggtc atatttctgg gaagatgcgg atgcattaca ttcgtatttc
    12901 tccgggagat aaggtcacag tagagctgac accttatgat ctaactaggg ctcgaatcgt
    12961 tttcagagca agataaacca ataaaaggaa aataaaatgc gtgtacaacc atctgttaag
    13021 aaaatttgcc gaaattgcaa gattattcgt cgaaatcgtg tagttcgtgt aatttgtact
    13081 gatctccgtc acaaacagcg tcaaggttaa tggaatattt cttttaatgt gattctgtga
    13141 tatagtgaca cactttgccc taaaaaggaa aaaatatggc tcgtattgca ggggtaaata
    13201 tccctaataa cgcacacatc gtaattggtc ttcaggctat ttacggtatt ggtgctactc
    13261 gtgctaaatt gatttgtgag gctgcaaata ttgcgcctga tactaaagca aaagatttgg
    13321 acgagactca attagatgct ttgcgtgacc aagttgccaa gtatgaagta gaaggtgatt
    13381 tgcgtcgtga ggtaactatg agtatcaagc gattgatgga catgggctgc tatcgtggct
    13441 tccgtcatcg tcgcggctta ccatgccgcg gtcaacgcac tcgtacaaat gcgcgtaccc
    13501 gcaaaggtcc gcgtaaagcg attgctggta agaaataaat tttaaggaat tttattaatg
    13561 gctaaagcaa acacagcttc acgtgtacgt aaaaaagtac gtaaaaccgt gagtgagggt
    13621 attgtgcacg ttcatgcatc tttcaacaat accatcatta caatcactga ccgtcaaggc
    13681 aatgcgttgt cttgggctac ctctggcggc gctggtttta aaggttctcg taaaagtaca
    13741 ccatttgcag cacaagttgc agcagaagca gctggtaaag ttgcccaaga gtatggcgtt
    13801 aaaaatttag aggttcgtat taaaggtcca ggtccaggtc gcgaatcctc tgtacgtgct
    13861 ttgaatgctc ttggtttcaa gattaccagc attactgacg ttaccccgtt gcctcataac
    13921 ggttgccgtc cgcctaaaaa acgtcgtatt taatattgga gtgatttgaa acatggcacg
    13981 ttatattggc cctaaatgta agttggcacg tcgcgaaggt acggatttgt ttttgaagag
    14041 tgcgcgccgc tctttggatt ctaaatgtaa aattgattcc gctcctggtc agcatggtgc
    14101 aaaaaaaccg cgtttgtcag actatggttt gcagttgcgt gaaaaacaaa aaatccgccg
    14161 tatttatggc gtattagaac gtcagttccg tcgttatttc gcagaagctg atcgtcgtaa
    14221 aggttctacc ggcgagttgc tgttgcagtt gctggaatct cgtttggata atgtcgttta
    14281 tcgtatgggt ttcggttcta cccgagctga agcaagacag cttgtttctc ataaggcgat
    14341 agttgtgaat ggacaagttg tcaatattcc ttctttccaa gtgaaagctg gtgatgttgt
    14401 ctcagttcgt gaaaaagcca aaaaacaggt acgtattcaa gaagcattgg gtttggcaac
    14461 tcaaatcggc ttgccgggtt gggtttctgt agatgcggat aaacttgagg gtgtgttcaa
    14521 aaacatgccg gatcgctcgg aattgaccgg tgatattaat gaacagctgg tggtagagtt
    14581 ctactctaaa taatgctagc tcagtgaggg acagttaaat gcagaatagc acaaccgaat
    14641 ttttgaaacc tcgtcaaatt gatgtaaata ctttttctgc aactcgtgca aaagtatcta
    14701 tgcagccatt tgaacgtggt ttcggtcata ccttaggtaa tgctttgcgc cgtatcttac
    14761 tgtcatccat gaatggtttt gctcctactg aagtagctat tgccggtgta ttacacgaat
    14821 attctactgt tgatggtatt caggaagatg ttgttgacat tttgctgaat attaaaggta
    14881 ttgtgtttaa actccatggt cgtagccaag ttcaacttgt gttgaagaaa tcaggttcag
    14941 gtgtcgtatc tgccggtgat attgagttgc cgcatgatgt agaaattctg aatcctagtc
    15001 atgtcatttg tcatttggct gataacggtc aaattgagat ggaaattaaa gtagagcaag
    15061 gtcgtggtta tcaatctgtt tcaggtcgtc aggtagttcg tgatgagaac cgtcagattg
    15121 gtgcaatcca gttggatgcg agcttttcgc ccatcagccg tgttagcttt gaggttgaac
    15181 ctgcacgtgt agagcagcgg acggatcttg ataagttggt tttggatatc gaaaccgacg
    15241 gttctattga tcctgaggaa gctgtacgca gtgcggcacg tattttgatt gatcagatgt
    15301 ctatttttgc tgatttgcag ggtacgcctg tggaggaggt tgaagaaaaa gcacctccta
    15361 tcgaccctgt tcttttgcgt ccggtggatg atctggaatt gacagtacgt tcagctaatt
    15421 gtttgaaagc tgaggatatt tattatattg gcgatttgat tcaacgcact gaaaccgagc
    15481 ttcttaaaac gccgaatttg ggacgtaaat ctttgaatga gattaaggaa gtattggcat
    15541 ctaaaggttt gacactgggt tctaagttgg aagcatggcc acctgtaggc ttggaaaagc
    15601 cttaatgaag aattaaagga taattgatat gcgtcatcgt aatggcaatc gcaaattaaa
    15661 ccgtaccagc agtcatcgtg ctgcaatgct gcgtaatatg gcgaattcat tattgactca
    15721 cgaagctatt gtaacaactc tgcctaaggc caaggaattg cgccgtgtag tagagccgtt
    15781 gattacattg ggtaaaaagc cgtcattggc aaaccgccgt ttggcatttg accgtactcg
    15841 cgaccgtgat gttgtagtaa aactgtttgg cgatttgggt cctcgtttta ctgctcgtaa
    15901 cggtggttat gttcgggtgt tgaaatacgg attccgtaaa ggtgataatg cacctctggc
    15961 actggttgaa ttggttgaca aaccggctgc tgagtaattt tagtcatata acgccatctg
    16021 ccgaaaagca ggtggcgtta tttttgcaat atctgatagg taatagggta ttggctatca
    16081 tgtttaaaat attaattgaa tagctaaggt ttgcgcggta aacttacatc attaaaaaat
    16141 tctatgatgg tttatataat gaatgctttc gatataaagt cgacaaagat ggacgtattg
    16201 tctatatctt tgcatacgtc agacttgttt gatttggaag atgtgctggt caaattgggc
    16261 aagaagtttc aagagtctgg tgttgttcca tttgttctgg atgttcaaga gtttgattat
    16321 cccgagtctt tggatcttgc tgcattggtt tcgttgtttt caaggcatgg tatgcaaatt
    16381 ttgggtctga agcattctaa tgaacgttgg gctgctgcgg ctatgaagta tcatttgctg
    16441 ttttgtctgt ctcattcgga aaatgttaaa gaactgggtc aggttgaggt gcagaacacg
    16501 gaggatggtc agaaagcaag gaaaacagta ttgattacat cccctgtccg taccggtcag
    16561 caggtttatg ccgaagatgg cgatttgatt gttacggggg cggtcagcca gggggcggaa
    16621 ttgattgcgg atggcaatat acatatttat gcgccgatga gggggcgtgc tttggccggt
    16681 gccaagggtg atacttctgc ccgcatattt atccactcca tgcaggcaga actggtttct
    16741 gtggcgggta tttaccgtaa ttttgaacag gatttgccga accatctgca caagcagccg
    16801 gtacagatat tgttgcagga taaccgattg gttatcagtg caattggctc agagtaattg
    16861 tttgatattt aaaaaggaaa tattgtggca aaaattattg tagtaacttc aggtaagggc
    16921 ggtgtcggta aaacgactac cagtgccagt attgcgacag gtttggcatt acgcggatat
    16981 aaaactgcgg taattgattt tgatgtgggt ttgcgtaacc tcgacctcat tatgggttgc
    17041 gagcgtcgtg tcgtttatga cctgatcaat gtcattcagg gggaggcgac gctcaaccag
    17101 gctttgatta aagataaaaa ttgtgaaaac ctgtttattt tgccggcttc ccagactcgg
    17161 gataaagacg ctttgacacg cgagggcgta gaaaaagtga tgcaggagct gtccggcaag
    17221 aaaatgggct ttgagtatat tatttgcgac tctcctgccg gtattgagca gggtgcattg
    17281 atggcgttgt attttgctga tgaagccatt gtaacgacca atcctgaggt ttccagtgtg
    17341 cgtgactccg acaggatttt gggaattttg caaagcaaat cccataaggc agagcaaggc
    17401 ggttcggtta aagaacatct gttgattacg cgttattctc ccgaacgtgt ggcaaaaggc
    17461 gaaatgctgt ctgtacagga tatttgcgat attctgcata ttcctttgct gggtgtgatt
    17521 cctgaatccc aaaacgtctt gcaggcatcc aattccggag aaccggtcat ccatcaggac
    17581 agcgtggcgg cttccgaggc atataaggac gttattgccc gtcttttggg cgagaaccgt
    17641 gaaatgcgtt tcttggaagc tgagaaaaaa agcttcttca aacgtctgtt tggaggataa
    17701 ggtatgtcat taatcgaatt tttattcggc agaaagcaga aaacggcaac cgttgcccgc
    17761 gaccgccttc aaatcatcat tgcccaagag cgcgcccaag aaggtcaggc tccggattac
    17821 ctgccgactt tacgtaaaga gttgatggaa gtcctgtcca aatatgtgaa cgtttcatta
    17881 gacaatatcc gtatttccca ggaaaaacag gatggtatgg atgtgcttga gttgaacatt
    17941 actttgccgg aacagaaaaa ggtataggac atgaccttaa ccgaattgcg gtacatcgtc
    18001 gcagtcgccc aagaacgtca tttcggcagg gcggcgcggc gttgttttgt cagccagccc
    18061 actttgtcta ttgccattaa gaaattggaa gaagagcttg ccgtctcttt gtttgaccgg
    18121 agcagcaacg atattattac gaccgaggcg ggggaacgta tcgttgcaca ggcgcgtaag
    18181 gtgttggaag aggcagagct tatcaggcat ttggcaaatg aagaacaaaa cgagctggag
    18241 ggtgcgttca aactcgggct gatttttacg gttgcgccgt acctgctgcc gaaactgatt
    18301 gtctcgttgc gccgtactgc accgaaaatg cctttgatgt tggaagagaa ttacacgcat
    18361 actttgaccg agtcgctcaa acgcggggac gttgacgcga ttatcgttgc cgaaccgttt
    18421 caagagccgg gcattgttac cgaacccttg tatgacgaac cgtttttcgt gattgtcccg
    18481 aaagggcatt catttgagga actggatgcc gtttcgccac ggatgctggg tgaggagcag
    18541 gttttgctgc tgacggaagg caactgtatg cgggatcagg tactctcaag ctgttccgaa
    18601 ttggcggcga aacaacgtat acaggggttg accaatacat tgcagggcag ctcgattaat
    18661 acaatccgcc atatggttgc cagcggtttg gcaatcagcg tgttaccggc aaccgcgctg
    18721 accgaaaacg atcatatgct gttcagcatt attccgtttg aaggtacgcc gccaagccgg
    18781 cgggtcgtat tggcgtaccg ccgcaatttt gtccgtccga aggcgttgtc ggcgatgaag
    18841 gcggcgatta tgcagtcgca gcttcacggg gtaagtttta tccgcgacta ggcgcaggca
    18901 ttgttttcaa aacgccattt ccctgagccg acaacacggt atgccaagat attgccgtca
    18961 tcatcgattt tgagtatagc atcgccacgg aaactgccgt cctgaagata ttcgactttt
    19021 gcatcactgt gaatgttttc atcagtgccg atgcaatgcc atgtatagtg gattaacaaa
    19081 aaccagtacg gcgttgcctc gccttgccgt actattgcca aggcgtacca gcctgatttg
    19141 ttgatgtgtg tactactgca tgggttgtta agccctgcaa gtgatttaag tgtgaagatt
    19201 gagtaatgcc cactagtcgc aggcgcgggg ggcaggtgct aagtcattgg ttatctcctt
    19261 actatctatc taactacagg gtgtattatg cgcctccttg ttgttttgtg caagtggttt
    19321 tctgataatt tatgtaaaga tttggtaaag acgtatagcc tttttctttg cattgtgctt
    19381 gcgcagggta tcaggtaggt ctcaaaaaat ttgaaaattt ttatgttttc gtataaaatc
    19441 ttttgcactt atacggagtg agttatgcgg tacaccatta aaacatttca aacccttgct
    19501 gatgaaactt tcccctctaa gaacttcaaa attctttcgt tcaatggggt ggcacgacct
    19561 ataaccattg tttgccctac acacggggtg cagactgttt ctgcggggca agcatttatc
    19621 cgttccaaac acggctgtcc tgcctgtggt ttagttttat caacctccgt tttggcctct
    19681 aggggtcggt cagtctccat cctcgacacc gccacaggcg aaacactctc gttccccagc
    19741 gtacaagctg ccgccaaagc cctaaacacc tcctacggct ctatacgaac caagctcgac
    19801 ggacgttcgt cccccgacaa ccttgtctgt aacaggtaca aagtaatgcc gtaatctcga
    19861 aacttcgtgc tataataaac tcctagcttt tgcaggagtt tgttttttat ggatttaaat
    19921 gtcgaaatca agaattttgg caaggttgcc catgctaggg tttccattcg cccgcttacc
    19981 gtcatcacgg gttgcaactc cacactcgcg ttccccagcg ttactgccgc aggcactgct
    20041 ttgggtgtgc attttcagca aattaaccac aggctcaagg gtcgaacgtc ctgtcaggag
    20101 gtgttgattt ctacaccgat tatactgtct gcggcttcgt cgccttgtcc tgatttttgt
    20161 taatccacta taaaagaggc cgtctgaaaa acatttttca gacggccttg tttattcaat
    20221 caaatcagtc tttcaacttc gccaactgat tttgaacttt tgccattttg tcttccaatt
    20281 ccgccaaatc ggctttgtct ttttccacca gatgcgcagg ggctttttcg gtgtagccgg
    20341 gtttggagag tttggcgttg agtttgtcca aggctttttg cagcttctcg gcttctttgc
    20401 tcaaacgggc ggtttccgca gctttgtcga tttcgacttt caacatcagg cgcgcgccgt
    20461 tgcagacggc gacgggcgcg tcttcgcttt cgggtagggc ggcgacttgc tgtgcttcga
    20521 tcaggcgggt catcatcggc aggtatttga ggtagtccgc caagtcgtcc gtgctttcga
    20581 caaacagcgg ggctttcaca ttgggctgga tgcccatttc gccgcgcagg ttgcggactg
    20641 cgccaatcaa atcctgcaac acggtcattt gctcgaatgc cgtctgaaca atatcgccgc
    20701 cgtcggcttc ggggaagcgg gcgagcatga tgctgtcggc ggttttcgcg tcgcacatag
    20761 gagcgacggt ttgccacaat tcttcggtga tgaacgggat aatcgggtgc agcaggcgca
    20821 gggcggcttc gagtacgcgc aacaaggtat ggcgtgtggc gcgttggcgg ctggcgcagc
    20881 cggtttgaag ctgcactttg gcaagttcca aataccagtc gcaatagtcg ttccacatga
    20941 agctgtacag ggtttccgct gccaaatcaa agcggtaggt ttcgtaggct tgcgttactt
    21001 gctcgatggt ctgattcaga cggtctacaa tccacatatc ggggaaggaa tagccgcgcg
    21061 gctcagtggc ggttgcgccg tagccgcagt cttggttttc ggtgttcatc aagacgaagt
    21121 tggtggcgtt ccagattttg ttgcagaagt tgcggtagcc ttcggcgcgt ttgaagtcga
    21181 agttcacgct gcgccccaag ctggcgtagc tcgccatggt gaagcgcaaa gcgtccgcgc
    21241 ccatactcgg aatgccttcg gggaagagtt ttctcgaggc ttcttccact ttcggcgcgg
    21301 tttcgggttt gcgcagaccg gtggtacgtt tcaccagcag tttttccaag tcgatgccgt
    21361 cgatcaaatc cacagggtca atgacgttgc cttcggattt ggacattttt ttgccttcgt
    21421 ggtcgcgcac gatgccgtgg atgtacacgt ctttaaacgg tactttgccg gtaaagtggg
    21481 tggtcatcat aatcatgcgc gccacccaga agaagatgat ttcgtagccg gtaaccaaga
    21541 cgttggacgg caggaaggct ttgagttcgt cggtttcaga cggccagccg agcgtggaga
    21601 acggcacgag tgcggaggag aaccatgtgt ccaatacgtc ttcttctcgg gtcaagcctg
    21661 ttttgccggc ttgtttttcg gcttcttcct gattgcgggc aacgtacaca ttgccttcgt
    21721 tgtcgtacca tgcagggatt tgatgccccc accacagttg gcgcgagatg caccagtctt
    21781 ggatgttgtt catccattgg ttgtaggtgt tgacccagtt ttcggggata aagcgcaccg
    21841 cgccgctgtc gacggctttt ttggctttgt cagcaaggct taagcctttg aattcgctgt
    21901 caggctcgcc gccgtttggc gtggcggaca tggcgacaaa ccattggctg gtcagcatcg
    21961 gctcaatcac ggaaccggta cggtcgcctt tcggcgtcat cagcgtgtgc gctttgattt
    22021 cgaccaagaa accttgttcc tgcaaatcgg caaccatttg tttgcgcgcg gcaaagcggt
    22081 ccaagcctgc gtatttttca ggcagggcaa agcttggttg cgcttcgcct ttgaagttga
    22141 acacttcggc gtttgccaat actttggctt ccaagtcgaa cacattaacc aagcgcgtat
    22201 cgtggcgttt gccgacttcg tagtcgttga agtcgtgcgc aggcgtgatt ttcacgcagc
    22261 cggtgccgaa gtctttttca acgtattcgt cggcaatcac gggaatggtg cggccggtca
    22321 gcggcaggat taattctttg ccgattaagt gggtataacg ttcgtcttcg ggattgacgg
    22381 caacggcaac gtcgcccaac agcgtttcgg gacgggtggt ggcgacgatg acggcttcgg
    22441 caggattgtc cgccagcgga tagcggatgt gccacataga gccttgttct tccacgcttt
    22501 ccacttccaa atcagacact gcggtaccca ataccggatc ccagttcacc aagcgtttgc
    22561 cgcgataaat caagccctgt tcatacaggc gtacgaacac ttcggttacg gtttcggcgc
    22621 gtacatcgtc cattgtgaaa tactcgcgcg tccagtcggc agaacagccc acacggcgca
    22681 tctgctgcgt aatcgtgccg ccggaaactt ctttccattc ccacactttt tccaagaatt
    22741 tttcacgacc caagtcatga cgggacacgt tttgcgcggc aagctgacgc tcgaccacga
    22801 tttgcgtggc gatgcccgcg tggtcggtgc cgggaatcca ggcggtgttg cagcctttca
    22861 tacggtagta gcgggtcagg ccgtccataa tggtttggtt gaaggcatga cccatgtgca
    22921 gcgtgccggt tacattgggc ggcggcagtt ggatggaaaa agacggtttg gtcaaatcca
    22981 tatcaggttg gaaatagccc tgctcttccc agttttgata atgtttggat tcgatttcgg
    23041 cgggggagta tttgtctaac atgatggaac tttgtgaaat taaggttatt tttgatgtgc
    23101 ggattataac gcaaaaaggc cgtctgaatc atttcagacg gcctttggca tacaggtttt
    23161 aaaaatggaa caataccagg ctgacggcaa tcaccgccat gcccattgtc aggccgtaaa
    23221 cggtttcatg gccgtctgaa tagcgtttgg cagccggcag cagctcgtcc aacgccaaaa
    23281 acaccatcac accggctatc acgccgaata ccgaaccaaa cacggcaggc gacaaaaacg
    23341 gctgcaaaac caaatagccc aaagccgccc ccaacggctc ggccaagccg gatagcagac
    23401 acgcccacac cgttttctta cggctgcggg tggcaaaata aaccggcgcg gcgatggaaa
    23461 tgccctccgg aatattatgg atggcaatcg ccaaggccaa aggcatcccg actgctggat
    23521 tttccaatgt ggcaaaaaac gtcgccaagc cttcggggaa attgtgcgca gtaatcgcaa
    23581 acgccgccat catgccgact cgcgcgatat ggcggcgttt gctttcttga aacgacgggt
    23641 cttgcgcgtc taaagtttca tgcgggttcg gcaccagacg gtcaatcagc gcaatgccgc
    23701 ccatccctgc caaaaatgcc atggtcgccg ccgcaaacgc gtggtcttta tcataaattt
    23761 cagcgaacgc ctcgctggac ttactgaaaa tctccgtcag ggaaacatac accatcgcac
    23821 cgccggcaaa tgccaaacca aacgacaaca cgcgcggatt gggcgttttg gaaaacatca
    23881 ccaagccgct gcctaatacg gtaaacaaac cggcagccaa cgtaatggaa aaggcaacgg
    23941 ccaaattgga catcgaaaaa tcgggcatga gaaaacctgc gctaaaagct gggacaggtt
    24001 cagactaaca ctttttaatg tatatgataa tagttattat ttattttatt gattggatac
    24061 acggattttg aaacaaaagg ccgtctgaaa aatgattttc agacggcctt taaatttgaa
    24121 atgccgctaa accttagtgc tttccagctt aagcctgata acgcgacagg ctcaaatcgt
    24181 cgctgcggat ttcggtgtct ttgccgctca cgatatcggc ggttaatttt gccgaaccca
    24241 gcgacatggt ccagcctaaa gtaccgtggc cggtattcag aaacaggttg tcaaagcggg
    24301 tgcgaccgat taacggcgtg ctgtcgggcg tcatcggcct gaggccgctc cagaacgatg
    24361 cttggctcaa atcgccgcct tccgggaaca agtcgttgac gaccaaagcc aaggtttcgc
    24421 ggcgtttttc gggcagtttg atttcgtagc ccgacaattc cgccataccg ccgacgcgga
    24481 ttctgttgtc aaagcgcgtg atggcgactt tgtagctttc atctaaaacg gtggacaccg
    24541 gcgcgccgtc ggaatttgtg accggcaggg tcaaggaata gcctttgacg ggataaatgg
    24601 gcagattgag atccaactgc gccaaaaccg tcctgctgaa acaaccgagc gcgcagacaa
    24661 cggcatctgc ttcaaaccgc cctgtttcgg tttcaacggt tttgatgcgc agcccgttgt
    24721 ggtcgatgcg gctgatggtt tggttgaaat gaaaccgtac gcccttttcc tgacacaatt
    24781 tgtataggtt ttcagtgaag aggcggcagt cgccggttgc atccgcaggc aggtgcaggc
    24841 cgccggcaat tttggcggta acgcgtgcca gcgcaggctc gaattctgcg cattcttcgg
    24901 gcttcagacg gcggtacggc acgccgtagc gttccaaaac ggcaatgtct tgttctgcag
    24961 cttcaacttc tttagcctga cggaaaatct gcaacgtccc ttttttgcgc ccttcaaaat
    25021 tcatgccggt ttgcgcttca aaacggcgga acatttcacg gctgtattcg gaaatcctga
    25081 ccatgcgctc tttattggtt tgatagtgcg ctgccgtgca gttttgcagc atccgccaca
    25141 gccattcgat ttgatacagg ctgccgtcgg ggcgaaacag caaaggcgga tggcttttaa
    25201 acagccattt cagtgctttg gtcggaatac cgggtgcagc ccaaggcgtg gtatagccgt
    25261 aagaaagctg gcctgcattg gcaaaactgg tttccatcgc cacacccttg gcgcggtcga
    25321 tgaccgttac ttcatgtccg gcctctgcca gataccacgc ggaagacacg ccggcaacac
    25381 ccgcacctaa aacaagcact ttcatgtttc tccctccgac tttttcaaaa cagacttaat
    25441 atgccgtgcc gtctgaatat tcggattcag acggcctcgg atattaatgc ggcaattcgc
    25501 cgtttgtgat tttttgtttg aagtcgcgcg tttcattgac gatgactttc gccatcaata
    25561 aaagtgcaat caggttgggc aatgccatca agccgttgaa tgtgtccgaa gccagccaca
    25621 ccaaatcaag gctcaacacg gtacccagca taacggaaga aacataaccc acgcggtaca
    25681 aaccggcaaa tttctcgccg aaaacataca ccgcgcattt ttcgccgtaa tagcaccagc
    25741 ccaaaatggt tgagtaggca aagaaaatca ggccgatggt aacaatccag ccgccgatgc
    25801 cgggcagcat tttttggaat gtgacggttg tcagtgccgc gccgctcact tcaggtttga
    25861 caaactcgcc gcccgcgccg agcagtccca ttaccaacac gatgccggta atcgagcaaa
    25921 cgacgatggt atccaaaaac gtaccggtca tagaaaccaa ggcctgacgg acgggatggt
    25981 cggttttcgc ggctgcggcg gcaataggcg cagaacccat acccgcctca ttggagaaca
    26041 cgccgcgcgc cacgccgtag cggatgaccg taccgatagc gccgcccgcc actgcctgcg
    26101 cgctgaacgc atcggagaaa atcagcttga cggcaggcat cagtgcatcg gaattaatcg
    26161 cgataatgga aagaccgccc aacacataaa acaccgccat agcaggcacg atgaaagaag
    26221 cggctttggc gatgccttta ataccaccta aaacgacaac ggcagtcaga acggtcaacg
    26281 taatgccggt ataggcaggt tcgataccga agctggtttg caccgcctgt gcaaccgagt
    26341 tggactgcac cgagctgccg ataccgaagg aagcgaatgt gccgaacagc gcaaatgcaa
    26401 cggccatcca tttccagttt ttgcccaagc ctttttcgat gtaatacatc gggccgccgg
    26461 acatttcgcc tttggaattg ttgacgcggt atttcaccgc caacacgcct tcgccgtatt
    26521 tggtggccat gccgaaaatg gcggtcatcc acatccaaaa taccgcgccc gggccgccgg
    26581 ttaccaccgc agtcgccacg ccggcgatgt tacccgtacc gatggtggcg gacagcgcgg
    26641 tcatcaacgc cgcaaaatgg gaaatatcgc cttcgtggcc ttcgccgcct tcatacttct
    26701 ttgacggcac aaacgcctgt ttcagcgcat agcccaacat agtgaactgt aagcctttca
    26761 gcataacggt cagcacaatg ccggtgccga ccagcagcat caacataaca gatccccaga
    26821 cccagccgct gacggtttcg aaaaaggctt tgagattgtc taaaaacaat tgcatggctt
    26881 tctcctttgt ctgttttatt tttaaaacac cacttttgta gtgtcctgta atttcagcac
    26941 agaatattca ataagacaat atgttctttt gaaaaatact tttggttttt tctccgaaag
    27001 caggtcggtt caagctgcgg aaattgtttg caattcttta aaagcagcgg cggaggtcac
    27061 aatgaaatgt ccgaatgggg atgtggcggg cggcagaaat catcaatgct gccgactgcc
    27121 atacttctga aatctacaaa atgatgcatc gatcaaacaa tataccgctt taaaaaaacc
    27181 gatgccgtct gaaacgcttt cggggtttca gacggcatca aaagggtacg gtcagcggat
    27241 gatgccgcgc gccgattgtg cgaaaaagtc tcggaatacg gcaagctcgg cttgggtttc
    27301 ggcgcggcgg agaatgtctg ccttggcttc ttcaaacgga atgccgcgat ggtagagggt
    27361 tttgtacacg tctttgacgg cggaaatctg ctctgcggta aaaccgttgc ggcgcatgcc
    27421 ttcgctgttg agccccgccg gttcggcgcg gtagcccgat gccataaagt agggcggcac
    27481 gtctttgtgt acgcctgcgg caaacgcggt catggcgtag tcgccgatgc ggcagaattg
    27541 gaaaaccagc gtgtagccgc ccaaaacgac gtagtcgccg acggtaacgt gtccggcaag
    27601 cgaggcgttg ttggcgaaga tggtgtggtt gccgaccacg cagtcgtgcg cgaggtggca
    27661 gtacgccata atccagttgt cgtcgccgat acgggtttcg ccgatgccgg ttaccgtgcc
    27721 taaattaaag gtggtgaatt cgcggatggt gttgccgttg ccgataatca gcttggtcgg
    27781 ctcgtcgcgg tattttttgt cctgcggaat ttcgccgagg ctggcaaatt ggaaaatgcg
    27841 gttgttttcg ccgatgctgg tgtggccgtt gatgacggcg tgcggaccga tttcggtatt
    27901 cgcgccgatt tggacgttgg ggccgataac agtgtacgcg ccgactttga cgccggagtc
    27961 gagttcggct ttggggtcga tgacggcggt cgggtggatg agggtcatgt ttttcctttc
    28021 ctgtcgtgtt gccgcgaaga tgcgcgacgg caacaggtcg tctgaaaact ttcagacgac
    28081 ctttttctga acactcaaac cacgcgtttg gcgcacataa tcacggcttc gacggcgact
    28141 tgcccgtcca ctttggcaac ggcgttgaat ttgccgatgc cgcgtcggct ggtcagcagt
    28201 tcgacttcaa agacgagttg gtcgccgggg atgacttggc gtttgaaacg ggcttcgtct
    28261 atgccggcga agaagaagaa ttcgttttct ttgcgcccgc cttcgctcaa aatcgccaac
    28321 gtgccgcacg cctgcgccat cgcttcgatg atgagtacgc cgggcatcac gggcaggtcg
    28381 gggaaatggc cttggaactg cggctcgttt atggtgacgt ttttaatcgc ggtcagggtt
    28441 ttcatcggct cgaaggcggt gatgcggtcg agctggagaa acggatagcg gtgggggatg
    28501 agtttttgga tgtctttggc ttcgatgggg agttgtacgt ccatgtctgt cgtattcctt
    28561 gaataaagtc ggtttggtta tttgctgtct tgaccggcat ctgaaagctg ctgctccagt
    28621 gttttgagcc gtttgttcat ttcgcttaag cggtggatgt aaacagcgtt gcgcgcccat
    28681 tctttatggg tggacatcgg gaagatgccg gcgaggtgtt tgccgctttc ggtaatgctg
    28741 tgggtgacgg acgtgccgcc gccgatggtg gttttgtcgg cgatttcgat gtgtccgacc
    28801 gtaccgacgc cgccgccgat gatgcagtag ctgcctatgg ttacgctgcc tgagatgccg
    28861 gttttggcgg cgatgacggt gtgcgaaccg attttgcagt tgtgtccgat ttggacttgg
    28921 ttgtcgattt tggtgccgtt gccgacggtg gtgtcgctca tcgcgccgcg gtcgatgttg
    28981 gtgttcgagc cgatttctac gtcgtcgccc agcgttaccg cgccggtttg cgggatttta
    29041 aaccatgaat cgccggcaaa agcgagtccg aaaccgtccg cgccgatgac cgcgccgctg
    29101 tggatttcga cgcgtctgcc cagtgtgcag ccgtaataaa cgacggcgtt gggatgcagg
    29161 acgacttcgt cgcccagttt gcaatcgtgt tggacgacgg cgtttgccaa gatgcggcag
    29221 ccttcgccga gcacggtgtt tgcgccgatg taggcgttcg cgccgatttc gcagcttgcg
    29281 ggaacggtgg cactcggttc gacgacggcg gtcggatgga tgccgccgcg cgctttgacg
    29341 acgggtgaaa acaggcgggc gactttggcg aaatagagat aggggtcgtc ggcgacaatc
    29401 aggttgcgcc cttcaaatcc gtctgccgct ttggcggaaa cgatgaccgc gcccgcgctg
    29461 ctgtcgtgga cttcggcttt gtatttcgga ttggcaagga agctgatgtg ttccgcctgc
    29521 gcgtctgcga gcgggcgcac ggcggtaacg gaaatgtcct cgccgcgcca ttcgccgccg
    29581 agccgcgcgg tgatttggga cagggtgtag gtggccggaa tcatggtttt cctgttcggt
    29641 atgccgtctg aaagggtcag cgggcgttca tttctttaat gacgctgtcg gtaacgtcgt
    29701 attgggtgtt gacgtaaatc acgttctgca aaatgacatc gtaaccttcc tgtttggcga
    29761 ttttgacgat gacgcggttg gcgttttgct ggagggaggc aaactcttcg ttgcggcgga
    29821 ggttgtagtc ttcttcaaac tgcgcctgtt ttttgcggaa cgctgcgacc agcccgcgcc
    29881 atttttcttc ggcttgcgcc ttttttgcgt ttctgagttt gccttcggca agctgccttt
    29941 ccaaatccag accttcgcgt tgcagttttt gcaattcgtc ctgacgggcg gaaaattcgc
    30001 tgtccagcgt tttttgaatc ttgcgcgcct gcttggattc gaggtagatg cgctcggtgt
    30061 tgataaagcc gattttttgg aaggtgtcgg cgtgcgcgcc tgcggtgcag cacaaaccga
    30121 tcagagccgc ggcaaacgcg cgggtcaaac gggtcatggt aaaactcctt cgaatgttgc
    30181 cgcgaaatgc cgtctgaagg gcttcagacg gcatttgcgg gattagaacg tcgtgccgag
    30241 ttggaattgg aagcgttgga tttcgtcttc cggttttttc ttcagcgggt aggcgtagct
    30301 gaatttcatc gggcctaaag gcgagagcca ggtaaccgcg ccgccggcgg aatagcgcaa
    30361 ttcgttggta aaggtggatt tatgggtatt gccggcgccg taaatgtttt gaaccctgcc
    30421 gccggtcgcg gaactgctgt tgtcgtcgta ggttttgccg tcccacacgc tgcctgcgtc
    30481 ggcaaacagg ctcaggcgga cggtgcgcgc gtctttcgcg ccgggcatcg ggaagagcag
    30541 ctcggcggag acgttggctt ttttgttgcc gccgtagctg attttttcgc cgtattcgtc
    30601 atagactttc ggaccgagtg tgccgctttc gtaacctcgt accgaaccca ggccgccgcc
    30661 gtagaagttt tcaaagaagg ggatttcttt ggttttgccg tagccgttgg catagccgat
    30721 ttcgccgcca agcatcagtg tgaaggtttt gctcaggggg aagaaccagg tttggttgtg
    30781 ggtggcggag tagtattgca gtttgctgcc gggcagggcg atttcggcgt tcacgcccgt
    30841 caggtagccg cgcgtcggcc ataacgcgct gtcggttttg ttgcgccccc agccgacggt
    30901 acctttgtac agccagcctt tgaagctgcc gtctgtgccg tcggttttgc cgtatttctt
    30961 gataaagtcg gcatagtgtt tgggcgcttt gttgtaggtg ttgacggtca ggtgttctgc
    31021 caccaaaccg aaattcacgc ggtcgtattc ggtaacaggc acgctcatgc ggatgcctgc
    31081 gcctgccgtg gtggttttat attgtttgat gctggtcgat gctttgcgcg ggtcgaaggc
    31141 ttttccgtaa acatcgtagc ccaggctgac cccgtctgcc gtgaagtacg ggtcggtaaa
    31201 cgacagcgag ccgttgagcg tggttttgct tcgtgaggcg cgcagggcgg ccgacttgcc
    31261 cgtaccgaac aggttgtctt gggaaacgcc tgcggacatg accaggccgg tatcctgtac
    31321 ccagcccgcg ctcaagtcga gcgagccggt ggaacgttcg gtcaggctca tgttcaaatc
    31381 gactttgtcg ggtgtgccgg caagcgggac ggcatcaaac tgtacgttgt cgaagtagcc
    31441 caaaagctcg acgcgctctt tggagcgttg cagcttggag gtgtcgtaag gcgcggattc
    31501 catttggcgc aattcgcggc gcacgacttc gtcgcgggtt ttgttgttgc cggtgatgtg
    31561 gatttcgttg acgtagattt tccggcccgg ttcgatgtgc aggacgaaat cgacggtttt
    31621 ggtttcggcg tttggcagcg gctgtacgct gatttcgctg tatgcgtagc ctgccgagcc
    31681 catgcggttc tgaatctcac ccaaaacggc ggtcatctgc tggcgttcgt accatttgcc
    31741 gggcttcatg gtcagcagtt tttccagttc ggctttgggg acttcgttgg tgtcgccttc
    31801 gatggagact ttgccccaac ggaaacgttc gccttcgtgg acggtgattt tgatggtctg
    31861 cttggttttg tcttcgttgg tttggatgtc ggtatcgagg atgcggaaat cgaagtagcc
    31921 gttgttttgg tagaagtcgg ttactttttc catgtcttgg gcaaatttct gctcgttgaa
    31981 ttggttgctt cgtgtcagcc atgtccaaat gccgccttcg gtcagcgaca tctgccgcat
    32041 cagtttgcgg tcggaataga cttggttgcc ttcaaattcg atgtcggtga ttttggcgga
    32101 tttgccctcg tcaatcgtga tgtcgatgtc gacgcggttg cgggcgagtt tggttacttt
    32161 gggcgtgatt tggatattga gtttgccgcg cccgaggtat tcttctttca ggccggcgac
    32221 tgcctgattg agtgtcgcct gattaaagta ttgcgactgc gccagcccga acgattcgag
    32281 atttttctta atggcgtcgt tttgcagcat ttttgcgccg gtgatgttga gcgagccgat
    32341 ggtggggcgt tcgatgacgg tcagcaggag ctgcccgtcc gcagtctcga cgcgtacgtc
    32401 gtcaaagaaa ccggtggcgt acaggctttt gatgatggca ctgccgtgtg tgtcgttgta
    32461 ggtgtcgccg actttgacgg gcaggtagtt gaatacggta ctcggctcgg tacgctgcaa
    32521 gccttcgacg cggatgtctt ggatggtgaa gtcggcaagt gccaaaggcg atatgcccaa
    32581 gaccatcagt gcggaagcaa tctgtttcag tttcattgtc agttccttgt ggtgcggaat
    32641 gcggtttcag acggcattcc gaaacgtaaa atctaaccga gcagccgggt aacgtcgttg
    32701 aagaaggcga ccgccatcat cagcatcatg agggcaagcc cgaagcgcaa accgatgttt
    32761 tggacgcgtt cgcccaaagg tttgccgcgt atccattcgg cagtataaaa cacgaggtgg
    32821 ccgccgtcca aaacggggac gggcagtagg ttcagcacgc cgaggctgat gctgaccagt
    32881 gccaaaaatt ccaaataact ttgcaagccg agttcggcgg actgtccggc aatgtcggca
    32941 atggtcagcg gcccggaaat atggctgacg gaggcgttgc cgctgattag tttgccgaaa
    33001 aatttgaggg ttgtccacga gtgggaaacg gttttttccc agcccatgcc gaatgcgcgg
    33061 ataacagacg gacggtagct gcggcggatt tgcgcgtccc acgccctgtc cggctgcgga
    33121 cggaggccga cgcgcccgat cagggtgtgg tcggactgtt cgacagtatc ggggcggatg
    33181 tcggcggtat gggtttgtcc ggcgcgttcg tagttcaggg tgattttttt gccggggctt
    33241 tggcgggtca ggtttgccca ttcttgccat gaggcgatgg gtttgccgtc ggcggcagtc
    33301 agcctgtcgc ccggtttcag gcctgctttt tcggcggggc tgcctttttc cacgccgccg
    33361 gcaacggttg tgattttaaa gggcatcagt ccgatgtagc cttggttttt tgcgatttta
    33421 ccggcttccg gcgtgcctgc ggcatcgatg gtgcggacgg tttgcgcgcc cgatgccgtc
    33481 tgaacgccga cggcgacttt gccggcttcg aggttgagga cgatttcggt ttgcgcgctg
    33541 ccccaatctg caacgggtgt gccgttgacg gattgtattt tgtcgccgct ttggaagccg
    33601 gcgcgggcgg caatggtgtc aggttcgact gtgccgacgt aggggcgcag ttcggttacg
    33661 ccgaaggaaa agctcagtcc gtacagcaaa accgccagtg cgaggttggt cagtgggccg
    33721 gcggcgacga tggcgatgcg cttggcgggg tgttgtttgt caaaagcgta gggtaaatcg
    33781 gcttctgata cttcgccttc gcgcgtgtcg accattttga cgtaaccgcc caacggaatc
    33841 ggggcgaggc accattcggt gtcgccgcgc tttcgggtga aaaacggttt gccgaagccg
    33901 acggaaaagc gtacgacttt gacaccgcac aatctggcaa cgatgtagtg tccgaactcg
    33961 tgcaggctga ccaaaatcag gatggcgaag ataaaagcta gaagggtgtg caaatggttt
    34021 tcctttgata acggtgttcg gacggcatca gcgcagtgtg ccgataaatg cccgcgcttg
    34081 tgcgcgtgtc cgggcatctt gcgccaagag cccccctatg tcacctatgc cgtctgaaaa
    34141 gtcttgtgaa agacaatggg cgacggtttt ggcaatgtcg gtaaacttaa tctgtccgtc
    34201 caaaaaggcg gcgacggcgg cttcgttggc ggcgttcaat acgcagggcg cgaccccgcc
    34261 cgcgttcatg gcttcatagg cgagcttcag gcaggggaag cggtcaaagt cgggcttttg
    34321 gaaggtcagc gcggacaatg cgtcgaaatc aaggtcgccg acacccgaat cgatgcgctc
    34381 gggcaagccc aaacaatagg cgatgggcgt tcgcatatcg ggattgccca gttgtgccag
    34441 cacggagccg tcgcggtagc gcaccatgct gtgtatcacg gattgcggat ggatgacgac
    34501 ttcgagtttg tcgggcggac agttgaacag ccaatgcgct tcaatcagct ccaaaccttt
    34561 gttcatcatg gtggcggaat cgacggagat tttgcgcccc atacgccaat tggggtgttt
    34621 gaccgcttgg gcgggcgtaa tgcggtcgaa cgtgcctaaa tcggtcgtta aaaacgggcc
    34681 gccggaagcg gtcaggataa tcgaacggat gccgtgttcg ttcagacggc cggcgtaatc
    34741 gcgcggcaaa acttggaaaa cggcgttgtg ttcgctgtcg acgggcagca ctgccgcgcc
    34801 gtttgcacgg gcggtttcca taaacaacgc gccggaaacc accagcgtct ctttgttcgc
    34861 cagataaatg gttttgcctt tttgcgccgc tgcgagcgcg gaaggcagcc ccaccgcccc
    34921 gacgatggcg cacatgacac cgctgacttc gtcggcagag gcaacgtcaa ccaatgcctg
    34981 cgcgccgtgt aaaacctgcg tcgccgtgcc gtcgcgtttc aacagggctt caagccgggc
    35041 ggcgtattcg gcatcggcaa cgacggcata ttcgggttgg aacgtttgac attgagccgc
    35101 cagtttctcg acctgcttat gccctgccag cgcgaatacg cggaattttt cggggtggcg
    35161 ggagacaacg tccagcgtgc tttcgcctat gctgccggta ctgcctaata tggtcaggac
    35221 ttgtggtgtc ataatgggga taactttata ccggatgccg tctgaagcgt tttcagacgg
    35281 catagaatca atttaaaacc gacatcatcg ccgcatagac gctgataacg gcaatcaggc
    35341 tgtcggtacg gtcgaacacg ccgccgtgtc cgggcagcag cttgctgctg tctttgatgc
    35401 ctgccgcgcg cttgagccag ctttccaaaa ggtcgccgca tacgctgaca acggtcaaca
    35461 ccaaaccgat taacacggta tcgaaccagc ctgtatcgaa tgccagccag ccggcacttc
    35521 gtacggcggt catgtacacg gccacgcaaa ccgcgccgcc gattgcgcct tcccagcttt
    35581 tgccggggct gattgccggt gcgattttgt gtttgccgaa cgccttgccg ctgaaatacg
    35641 cgcaaatatc ggcaacccac accaaaccca tcacggcgag cagcggcagg gcatcatcgg
    35701 gatgcgggcg cagggatacg agcgcgaacc aaaacggcat gaccagaagc cagccgacgg
    35761 cataaacctg ccaaccgccg ttgagcctcc atttgaatct caaccataaa ggcataacgg
    35821 cgagccaaaa tgccaaaaca acataccaaa ccaaattagg cagcatccag ccgcccgcat
    35881 aggcaaccac gccgaaaacc aaggttgcgg cgaggtaatg gttggtttta attttgcaca
    35941 aaccgcccat acgggcatat tcccacaagg caatcagggc aatcagtccg caaaatgcag
    36001 cccacaacca ttgcggcgcg taaaacagca tgcccagcat cagcggcagc agccacatgg
    36061 cggtcattac ccgttgtttc agcatattca gttcctttgc tgttcgatag gcagttgctc
    36121 ggaggtgcgt ccgaaccgcc gttcgcgttt ttggaacgaa gcgacggcat cgtccaaagc
    36181 cttgccgtca aaatcgggcc acaaaatatc ggtgaaatac agttcggcat aagccatctg
    36241 ccagagcagg aaattactga tgcgcgtttc gccgccggtg cggatgaaca aatccggctc
    36301 gggcgcgtct cccagcatca ggtattttgc cagcgtgtct tccgtaatct cggatacgcc
    36361 ttcggcaatc agtttgtttg ccgcctgcaa aatatcccaa cgtccgccgt aatcggcggc
    36421 aatgctcagg gtcaggccgg tattgtttgc cgtcaaggct tccgcctctt cgatgccttg
    36481 cagaatctgc cgattaaagc gttcgcggct gcccagtatt ttcaggcgca tattgttttc
    36541 gtgcaggcgg cgtacctgtt tttgcaaagc ctgtaaaaac agccccatca ggaacgaaac
    36601 ttcgtcttcg gggcggcgcc agttttcggt cgaaaaggca aatacggtca gatattgcac
    36661 gcccaatttg gcgcaatgcc tcaccatatt ttccaatgcg tccaaaccgc gtttgtgtcc
    36721 cattatgcgc gggaggaaac gttttttcgc ccaacggccg ttgccgtcca taatcacggc
    36781 gatatgcttg ggaatggcgg tgtgttccaa aacggcctgc gtgctgcttt tcatgtctgc
    36841 ctttcgcggt tcggcattca aatgccgtct gaacgccgaa ccgtgcaggt taaattgcca
    36901 tcaaatcttc ttctttggca gtcaggagtt tgtcggcttc ggtaatgtat ttgtcggtca
    36961 gtttttgaac cgcttcttcg ccgcgacgtg cctcgtcttc ggaaatttct ttgtctttga
    37021 ggagtttttt gatgtggtcg ttggcatcgc ggcgcacgtt gcggatggag acgcggcctt
    37081 cttccgcttc gccgcgtacg actttaatca ggtctttgcg gcgttcctcg gtcagcatgg
    37141 gcatcggcac gcggatcagg tcgccgacag ctgccgggtt cagtcccaag tttgaatcgc
    37201 ggatggcttt ctcgactttg gccgccatat tgccctcaaa cggtttcacg ccgatggtgc
    37261 gcgcatccag aagcgttacg ttggcaactt ggctgacggg gaccatgctg ccccagtatt
    37321 cgacttccac ttggtcgagc aggccggtat gcgcgcggcc ggtacgcact ttcgccagat
    37381 tttctttcag tacttcgacc gaacgctgca tcttgccttc ggctgttttt tgaatatcgt
    37441 tgatcatatt gttctttcgg taagataagg taagcgggca gccgtctgaa acgcgcttca
    37501 aacctttcag acggcatgaa aactgttaac tgcgaatagt accgttattc gggcatgacg
    37561 acaaggcagg cggattgggg atgccgtctg aagcggcagg cgtttcagac ggcatcgtgt
    37621 ccgaccgtca gccgtgttcc cgtgtttcaa gcaggctttg gcgcaggtgt tggcgttcgt
    37681 gggcatccag ccatttgcgg cgggtgcgtt gcagcaggat gacgagggcg gaaatttcct
    37741 gacgcatatt ggtgctgagc cagaggaagc cctgccattg gtagtggagg tgttcggcga
    37801 gggcttccag ttcggggttg atggcggtgt cgatgcggat gcggcgggcg tgtctgccgt
    37861 tgataagggc gacggtttgt tgcaggtcgg tttggagcag tgtgaagtgg cggtcaagca
    37921 gccggatttc gctgccgttg agtttgggag attgcagctt ggcggcagtg gtcaggagca
    37981 gctcggtggt gttgacaatt ttacggtggg cgtgctgcat ggcttccatc atggcggggc
    38041 tgatgcggct ttcgcccgat gtggcggcga ggtggctgcg gcttttgacc atgcgtgcgt
    38101 tgatttggcg cattttaacc atattctgct ccaaacgttc gcgcgtcata cgcctgccgt
    38161 tgctgatttc ggcaatcatt ttgctgcagt cggtcaggtt gtcggcaagc atgaaacgcc
    38221 acatcagtgt ggatttcagc ggcagcagtt tggcggcggc gatggcgatg gccgcgccga
    38281 tgaggacgtt catcgcgcgc atcaggccgc tgtcgaacca ttcgctgccg ttgtcgccga
    38341 tgagcatgca catcgtcagc cccgccagca tagggacgta gccgtttttg ccgaccgccg
    38401 cccagccggc cagtgcgctt gccgtgccga cggtgaggta gaagaggagg ttgccgtgga
    38461 aataatgctg gttcagccat aaaacgccca aacccgcgcc cagcccgatg accgtgccga
    38521 gcatacgttc caccgccttg gagtaaatcg cgccttgaaa ctggagcatg ccgaggacga
    38581 cgaagacggt catccctatc cactcgccgt gttggaggtg gagcagccgg gcggaggcgg
    38641 tggcgaacag aacggccccg ccgagccgga cggcgtggat gaggcggcgg tagcggtagc
    38701 gttcgtagga gttgagccag cggctgacga ggcggttgcg ttgcgaggtg ttcatatcgg
    38761 ttgccgtctg aagcggaaat gtgaaaaagc acaggcttcc cgagggaggg tctgtgcttg
    38821 gtattggtgc cggagaaggg aatcgaaccc ccgaccttcg cgttacgaat gcgctgctct
    38881 accgactgag ctacaccggc gtttttcgtt atgatatata tgaacggttg tttgtgcaac
    38941 ttttctggcg ggcggcaagg cagtgcgcgg tatagtg
//
LOCUS       DAWKOG010000004        37758 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.004, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000004 DAWKOG010000000
VERSION     DAWKOG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 37758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37758
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.004"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(142..232)
                     /locus_tag="WJK66_000145"
     tRNA            complement(142..232)
                     /locus_tag="WJK66_000145"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(196..198),aa:Ser,seq:gga)
     gene            complement(268..354)
                     /locus_tag="WJK66_000146"
     tRNA            complement(268..354)
                     /locus_tag="WJK66_000146"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(318..320),aa:Leu,seq:cag)
     gene            complement(390..476)
                     /locus_tag="WJK66_000147"
     tRNA            complement(390..476)
                     /locus_tag="WJK66_000147"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(440..442),aa:Leu,seq:cag)
     gene            565..2940
                     /gene="rnr"
                     /locus_tag="WJK66_000148"
     CDS             565..2940
                     /gene="rnr"
                     /locus_tag="WJK66_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEZ7577921.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKSDLVKCRIEAHK
                     DGFGFAVPLTPAKDGDFVLYERQMRGIMHGDIVTVRPAGMDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFGEACAKAAKKIPNHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKIGRNYRLVVAIADVSHYVRPDDAIDTDAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPHVERLCVVCDMVITYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSGGIEHPFKTQIDTLYKLFKILQKKRFERGAVEFDSIETQMLFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLAALREQL
                     GLLGLQLGGGDNPSPKDYAALAGQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEIFEGKISGVANFGIFVTLDDIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAIKVARADLDDGKIDFVLIAEGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAAGKKTARGGKVRGRGASAAAESGKKAKKPVPIKVKKRKGKS"
     gene            complement(3279..4742)
                     /gene="guaB"
                     /locus_tag="WJK66_000149"
     CDS             complement(3279..4742)
                     /gene="guaB"
                     /locus_tag="WJK66_000149"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEZ7577922.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVKWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSADKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(4883..7441)
                     /gene="glnD"
                     /locus_tag="WJK66_000150"
     CDS             complement(4883..7441)
                     /gene="glnD"
                     /locus_tag="WJK66_000150"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEZ7577923.1"
                     /translation="MPANLSSALETFKQQRDAAEAHYLKANRVSVFFREYTAAVETLL
                     AALWAEYFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGGIGELCESVRDDITGDTAFLEARFLFGNRQTADELAKKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATDLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNAKRAEDRLLFDLQPQVAESMGYEGLNLRR
                     QSEELMRVFYRAIKTVKQLGGILTPMLQSRVSSTPLRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRHRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILTVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGIADARQFAADHF
                     LTEEESDLLAWLVENHLLMSTVAQKEDIQDPSVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLTGNGGNPHTLFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILPKSNSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAEISSHSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERAEDSFTVFSLDLKNPKIQSSLKQTLLEQLS"
     gene            complement(7490..7810)
                     /locus_tag="WJK66_000151"
     CDS             complement(7490..7810)
                     /locus_tag="WJK66_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7577924.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLNLMTNSADTRQMPS
                     ESGI"
     gene            complement(8135..8608)
                     /gene="bfr"
                     /locus_tag="WJK66_000152"
     CDS             complement(8135..8608)
                     /gene="bfr"
                     /locus_tag="WJK66_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ7577925.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(8636..9100)
                     /gene="bfr"
                     /locus_tag="WJK66_000153"
     CDS             complement(8636..9100)
                     /gene="bfr"
                     /locus_tag="WJK66_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ7577926.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            complement(9563..10546)
                     /gene="lipA"
                     /locus_tag="WJK66_000154"
     CDS             complement(9563..10546)
                     /gene="lipA"
                     /locus_tag="WJK66_000154"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEZ7577927.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            complement(10539..11162)
                     /gene="lipB"
                     /locus_tag="WJK66_000155"
     CDS             complement(10539..11162)
                     /gene="lipB"
                     /locus_tag="WJK66_000155"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEZ7577928.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            complement(11164..11439)
                     /locus_tag="WJK66_000156"
     CDS             complement(11164..11439)
                     /locus_tag="WJK66_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HEZ7577929.1"
                     /translation="MTEQENKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            11624..12694
                     /locus_tag="WJK66_000157"
     CDS             11624..12694
                     /locus_tag="WJK66_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEZ7577930.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLFALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LAVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
     gene            complement(12801..13748)
                     /locus_tag="WJK66_000158"
     CDS             complement(12801..13748)
                     /locus_tag="WJK66_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HEZ7577931.1"
                     /translation="MEFFIILLAAVVVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVSALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(13763..14170)
                     /locus_tag="WJK66_000159"
     CDS             complement(13763..14170)
                     /locus_tag="WJK66_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEZ7577932.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVGKVETDSYQDLDAGQYAEILRHAGGNRYEVFYRGT
                     HWQAQNTGQEELEPGTRALIVRKEGNLLIIAKP"
     gene            14251..14910
                     /gene="ung"
                     /locus_tag="WJK66_000160"
     CDS             14251..14910
                     /gene="ung"
                     /locus_tag="WJK66_000160"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEZ7577933.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPAADVFNA
                     FRLTAFDRVKAVILGQDPYHGAGQAHGLAFSVRQGVRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKRKLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            14907..15116
                     /locus_tag="WJK66_000161"
     CDS             14907..15116
                     /locus_tag="WJK66_000161"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ7577934.1"
                     /translation="MNADIAVAAGVLKSRLICNFHLLGKTLWQSKKNQLYASLWAGCD
                     ELRGGMDASLYKELCAYAIVFKICF"
     gene            15013..15660
                     /locus_tag="WJK66_000162"
     CDS             15013..15660
                     /locus_tag="WJK66_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ7577935.1"
                     /translation="MHHFGRAATSCAAVWMRAFIKNYVLTLLFLKYVSDKHKYGGGMI
                     ELHAGTTFDDIVKLKNTADIGDRLNKIIAQIAEANDLKGVIDVTDFNDEDKLGKGKEM
                     IDRLSRLVGIFKKLNLSSNQAEDDDLLGDAYEYLMRHFATESGKSKGQFYTPAEVSRI
                     MAKIIGISADCRPAPAFMTRPAARVRCCSKPPPKPAAKSAFTVRKKMWQPRPLPV"
     gene            15733..16206
                     /locus_tag="WJK66_000163"
     CDS             15733..16206
                     /locus_tag="WJK66_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEZ7577936.1"
                     /translation="MKTTGLRPSILPLPIRLIPPEKNGDYAFLLHLLKSLKPSGKGAI
                     ILPHGVLFRGNAEARIRTELLNLDLIKGIIGLPANLFYGTGIPACIIVIDKEHAQTAQ
                     FAEEGTNQVISGGSVFMIDASRGFIKDGNKNRLREQDIHKIIDTFTNLVTAVWCI"
     gene            16554..17498
                     /locus_tag="WJK66_000164"
     CDS             16554..17498
                     /locus_tag="WJK66_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ7577937.1"
                     /translation="MAHPDYAAFKAGHLAKFAAWHTQNDLAAIQPGRLIRKWSESLLD
                     AFKPGSLIEEYDFYQILTDYWAETLQDDVYLIAQDGWKAVKNLAEITKESDEAANLTV
                     VFEETETDKKGKAKTKRISKKYRSEVIAPELVARRYFSDGIAKLEEKQSELERLSQEL
                     ENHIEEHGGEEGALNDVLDAKGKLSAKLLKTALEESGIEEGERAVLQTTQTLMTQEKA
                     AKDAVKTQIEALNLAVFKQFGRLSEAEIKQLAVQDKWLADLQSRIENRLENSIQQLIS
                     RLNTLEDRYRSPMAELAREVEKWQSKVNAHLENMGFGG"
     gene            17640..17774
                     /locus_tag="WJK66_000165"
     CDS             17640..17774
                     /locus_tag="WJK66_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HEZ7577938.1"
                     /translation="MTHLLERHRNARFMAHMDNFLPNWQSIKQQLNALELFAQIYNLT
                     "
     gene            complement(18064..18579)
                     /locus_tag="WJK66_000166"
     CDS             complement(18064..18579)
                     /locus_tag="WJK66_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7577939.1"
                     /translation="MKSKLPLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CHSTDLPPIGNYSSERYIPPQTPEPVSSPSNGGQVVKYKAPVKTVSKPAKSNTPPPQQ
                     APVNNSRRSILEAELSNERKALTEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(19045..20967)
                     /locus_tag="WJK66_000167"
     CDS             complement(19045..20967)
                     /locus_tag="WJK66_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ7577940.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALIKGKITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERVAPAHLDSEFSFEFYHPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIIRSEKLNIGYFAQHQLDTIRADQ
                     NPVWHIQQLSPEVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIAVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRA
                     EKEMAQLSEIQTACEAFLAQEDAYLEANKEKLQNTLSLLTKVKTQLAQIEEVWLACQE
                     ELERIETEIEKQFAER"
     gene            complement(21044..21439)
                     /locus_tag="WJK66_000168"
     CDS             complement(21044..21439)
                     /locus_tag="WJK66_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEZ7577941.1"
                     /translation="MMRRRGKKMLFEENPIDGQFAEYECGAGGIRLTGQSFHKPVLVH
                     KDSVCLSQCRTLSDLTPENLLSDIKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGIS
                     VECMNTDSAFRTLVFLHSEGRRAWAWLQP"
     gene            21612..22919
                     /locus_tag="WJK66_000169"
     CDS             21612..22919
                     /locus_tag="WJK66_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEZ7577942.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREIRISAVCD
                     LSEEKARQTCPSAAFVKDPFELVAREDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEEFGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            22912..23346
                     /locus_tag="WJK66_000170"
     CDS             22912..23346
                     /locus_tag="WJK66_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7577943.1"
                     /translation="MTEPKHEMPTEEQVAARKKAKAKIRTIRIWAWVILALLALTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLAVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVADLKSE"
     gene            complement(23405..23674)
                     /locus_tag="WJK66_000171"
     CDS             complement(23405..23674)
                     /locus_tag="WJK66_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEZ7577944.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(23856..26318)
                     /gene="lon"
                     /locus_tag="WJK66_000172"
     CDS             complement(23856..26318)
                     /gene="lon"
                     /locus_tag="WJK66_000172"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEZ7577945.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIEAVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMSKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDTDHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGSDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELLVEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DKKRLSETKKTSKAKPKAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLASDFYEKKDIH
                     VHVPEGATPKDGPSAGIAMTLAMVSAFSKIPVRADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNVKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEP
                     SGAEAAAESASKPKPRSRATKH"
     gene            26403..26594
                     /locus_tag="WJK66_000173"
     CDS             26403..26594
                     /locus_tag="WJK66_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase"
                     /protein_id="HEZ7577946.1"
                     /translation="MEFDAMPSETFRLQTAWAWKDKAGTKNCSVLPLLAVPSVWFAVL
                     PPYFQNMTQVFHVFFYFSR"
     gene            26569..27672
                     /locus_tag="WJK66_000174"
     CDS             26569..27672
                     /locus_tag="WJK66_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ7577947.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWHYVRESGANGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKRVLKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     GSIHVPFGVFSPLRGEYLDLLAHAPSTGFQTALDIGTGSGVLAAILAKQGIPSVIGTD
                     TNPKAVACARANIARLGFEKQVEIRETDLFPEGFVDLIVCNPPWLPAKPTSAVESALY
                     DPESAMLAAFLRDAPKHLNPDGEIRLIISDLAEHLHLRPSDFLDKAFAQAGLRVADVL
                     KTKPVHKKAADPNNPLAFARNRETTFLYRLKKA"
     gene            complement(27700..29445)
                     /gene="recD"
                     /locus_tag="WJK66_000175"
     CDS             complement(27700..29445)
                     /gene="recD"
                     /locus_tag="WJK66_000175"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HEZ7577948.1"
                     /translation="MERQTDEFAQAAARAAIRFLEHYAGSGDEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     GTSAPDATGARQNLAKWFQGAGSEGQRDAAALALLQSFMVITGGPGTGKTTTVAKLLA
                     LICGEDENLPHIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFDHIRPLPFDVLIVDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAHNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLTAWRQDAEDFNEAYCSHVRRKMNIPEHQAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPEFEPAFAMTVHKSQGSEY
                     REVWLLPPSAAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGEETFRQAAATVKTR
                     QTALGSMLERVFSQE"
     gene            complement(29513..30208)
                     /gene="lolD"
                     /locus_tag="WJK66_000176"
     CDS             complement(29513..30208)
                     /gene="lolD"
                     /locus_tag="WJK66_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEZ7577949.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVRVLHGLDLEIRAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSREEAAETAMSMLDKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFGRVMVM
                     KDGSLHPKQGANA"
     gene            complement(30201..31448)
                     /locus_tag="WJK66_000177"
     CDS             complement(30201..31448)
                     /locus_tag="WJK66_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEZ7577950.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQIRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTAKLIPEAQRDTVWVRDWTFSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPGGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSRRASKTQPAEALRYE"
     gene            31654..31932
                     /locus_tag="WJK66_000178"
     CDS             31654..31932
                     /locus_tag="WJK66_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7577951.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSGSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            complement(31988..32605)
                     /gene="recR"
                     /locus_tag="WJK66_000179"
     CDS             complement(31988..32605)
                     /gene="recR"
                     /locus_tag="WJK66_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEZ7577952.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVDHCARCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(32672..34210)
                     /locus_tag="WJK66_000180"
     CDS             complement(32672..34210)
                     /locus_tag="WJK66_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEZ7577953.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISDHSINNAIQNEQADGGGPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPKAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPANEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLIGLP
                     APVIVEVQAVTPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(34264..34638)
                     /locus_tag="WJK66_000181"
     CDS             complement(34264..34638)
                     /locus_tag="WJK66_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEZ7577954.1"
                     /translation="MIILHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQRPSEAEICS
                     WLEQVPLATLLNKRGTSWRKLDAETQQKVLSSTAEAVKLMSEMPSLIKRPVLECGGKV
                     YASFSEETYDGIFNRQAPCRQG"
     gene            34788..36416
                     /locus_tag="WJK66_000182"
     CDS             34788..36416
                     /locus_tag="WJK66_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEZ7577955.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNATMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGQDGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALEKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            36488..37741
                     /locus_tag="WJK66_000183"
     CDS             36488..37741
                     /locus_tag="WJK66_000183"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEZ7577956.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYVGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYKFEIAEETIKLMRQM
                     VENGEADALVAERVWQEFAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVRKVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRSQGKAHLIAEQI
                     DRARLAQIAPLQKAFRAAQDKTEKH"
BASE COUNT         8494 a         9752 c        10227 g         9285 t
ORIGIN      
        1 taccgcaaaa cataccacaa aaaacggtaa cggatagttg taagcggttg atgacgatta
       61 tagacaataa cgggttttcc caatgaaatt attgtttgaa taataaaaaa tcccaaaccg
      121 taaaagtttg ggatttgtat ttggcagaga ggaagggatt cgaaccctcg atacgctatt
      181 cacgtataca cgctttccag gcgtgcgact taaaccactc atccacctct ctaatggcgg
      241 aaattatccc aatcggaata atttatttgg tgcccgggag aagactcgaa cttccacacc
      301 cgtgaggata ccagcacctg aagctggcgc gtctaccaat tccgccaccc gggcaatcta
      361 aatattgaaa taaagcaaag catttgattt ggtgcccggg agaagactcg aacttccaca
      421 cccgtgagga tactagcacc tgaagctagc gcgtctacca attccgccac ccgggctttg
      481 ctttatttgc tgttttgcgg tacagtgtcc tgccgcaaag aagtcgctat tatatagttc
      541 ataaagagaa tgtcaacagt ccaaatgaat aaaaatatta aatctttaaa tttacgggaa
      601 aaagacccgt ttttaagtcg tgaaaaacag cgttatgaac atcctttgcc cagtcgggaa
      661 tggataatcg aattgttgga gcgcaaaggt gtgccttcaa aaatcgaatc gcttgcgcgc
      721 gagctgtcga ttacggaaga cgagtatgtc ttttttgaac gccgtctgaa ggcgatggcg
      781 cgggacggtc aggttttaat caaccgtcgg ggcgcggttt gcgcggcgga caaatcggat
      841 ttggtcaaat gccgcatcga ggcgcataag gacggtttcg gttttgccgt gccgctcacg
      901 cccgccaaag acggtgattt tgttttgtat gaacgccaga tgcgcggcat tatgcacggc
      961 gatattgtca ctgttcgtcc tgccggcatg gaccgtaggg gccgccgcga agggacggtt
     1021 ctggatattg tcgaacgcgc gcaaagcaaa gttgtcggcc gtttctatat ggataggggc
     1081 gtggcgattt tggagccgga agacaagcgt ctgaaccaaa gcatcgtatt ggaaccggac
     1141 ggcgtggcgc gtttcaaacc tgaatccggt caggtcatcg tcggcgaaat tgaggtttat
     1201 cctgagcaaa accggccggc agtggcaaaa atcatcgaag ttttgggcga ttatgccgac
     1261 agcggcatgg agattgaaat tgccgtgcga aagcatcatt tgccgcacca attcggtgaa
     1321 gcgtgtgcca aagccgcgaa aaaaattccc aaccatgtac gcaaaagcga tttgaaaggc
     1381 cgcgtcgatt tgcgcgacct gcctttggta acgatagacg gcgaaacggc tcgagatttt
     1441 gacgatgcgg tgtttgccga gaaaatcgga cgcaattacc gtctggtcgt ggcgattgcc
     1501 gatgtcagcc attatgtccg ccccgatgac gctatcgaca cggacgctca ggaacgcagc
     1561 accagtgttt acttcccgcg ccgcgtgatt cccatgttgc cggaaaacct gtccaacggc
     1621 atctgctcgc tcaatcctca tgtcgagcgt ttgtgtgtgg tgtgcgatat ggttatcact
     1681 tacgcgggca atatcaaaga ataccgcttc taccccgccg tgatgcgctc tcatgcccgc
     1741 ctgacctaca accaagtttg gaaatggctt tcaggcggca tcgagcatcc gttcaaaacc
     1801 caaatcgaca cgctttacaa actcttcaaa atccttcaga aaaagcgttt cgaacgcggg
     1861 gcggtggagt ttgacagcat cgaaacccaa atgcttttcg acgacaacgg taaaattgaa
     1921 aaaatcgtcc ccgttgtccg caacgatgcc cacaagctga ttgaagaatg tatgttggcg
     1981 gcaaacgttt gcgcagcgga ttttctgttg aaaaacaagc ataccgcatt gttccgcaac
     2041 catttggggc ccacgcccga aaaactcgcc gccttgcgcg agcagctcgg tctgttgggg
     2101 cttcaacttg gcggcggcga caacccgtcg ccgaaagact atgccgcgct tgccggacag
     2161 ttcaaaggca ggccggatgc cgaattgctg caagtcatga tgttgcgctc catgcaacag
     2221 gcggtttacg aaccgcattg cgacggacac tttggtcttg cctacgaagc atacgcccac
     2281 ttcacctcgc ccatccgccg ctatcccgac ctgaccgtac accgcgccat caaagccgtg
     2341 ttgaatcagc aaacctacac gccaaaaaaa agctggcagg ctttgggcgt gcatacctcg
     2401 ttctgtgagc gccgtgccga cgacgccagc cgcgacgtgg aaaactggct gaaaacctac
     2461 tatatgcgtg acaaagtcgg cgaaatattt gaaggcaaaa tttccggggt ggcaaatttt
     2521 ggaatatttg tcactttgga cgatatccat atcgacggtc tggtgcatat cagcgatttg
     2581 ggcgaagact atttcaactt ccgccccgaa atcatggcaa tcgaaggtga acgcagtggc
     2641 atccgtttca atatgggcga cagggttgcc attaaagtgg cccgtgccga tttggatgat
     2701 ggaaaaatcg attttgtcct gattgccgag gggagcggca gagggcggaa agttaaatca
     2761 tccgcgtctg ccaaaccggc agggacggcg gggaaaggga agccgaaaac cgccgccggg
     2821 aaaaaaacag cccgaggcgg caaagtaagg ggaaggggcg cgtctgccgc cgcagaatcg
     2881 gggaaaaagg caaagaaacc ggttccgatt aaggtaaaaa aacggaaagg caaatcataa
     2941 tgctgacggg gcggcttggg gagggagggg catactttcc cgaagccgcc gcccgatttt
     3001 ttgatatggc ggccggcgca tcatttcagg ccaatcgaaa cgcccgtatt gaaagattgc
     3061 gtttatttcc accgccgttt taaaggccag cggtattcgg cagacggggc gcaaacggcg
     3121 ttcagacggc attttcattc tttcggcgtg tccgtccgaa tggctttgcc cgtccgcgca
     3181 atcagcccgt gcggcttgcc ttgaacggac aaaaaatgcc gtctgaaacc cgaaaatcag
     3241 gtttcagacg gcattttgcc ttgaaaaggc tgttcaaatc agcggtggta gttcggtgct
     3301 tctttagtga tttgaacgtc gtgaacgtgc gattcgctca tacctgcgga agtgatttcc
     3361 acaaattcgg ctttttcgtg catttcggca atattggcgc aacccaagta acccatactg
     3421 gaacgcagac cgccggtcag ctggtggatg atgttcacaa tcgggccttt gtaaggaacg
     3481 cggccttcga tgccttcggg gacgtatttg tctgcgctgt cggttttgtc ttggaagtag
     3541 cggtcggcag aaccttggct catcgcgccc aaggaaccca taccgcgata ggatttgtac
     3601 gagcggcctt ggtagagttc gatttcgccc ggcgcttctt ccgtgcctgc aaacataccg
     3661 ccgagcatga cgctgtacgc gcctgcggcg agggctttgg cgatgtcgcc ggagaagcgg
     3721 atgccgccgt cggcaatcag cggaacgccc gtgcctttga gggcttcggc aacgttgtga
     3781 atggcggtca gttgcggcac gccgacacct gccacgatac gggtggtgca aatcgatccc
     3841 ggaccgatac cgactttgac ggcatccgcg ccggcggcga ccaaatccaa tgcggctttg
     3901 gcagtggcga tgttgccgcc gatgacttgg atgtgtggat aggtttcttt cacccatttc
     3961 acgcggtcaa tcacgccttg gctgtgcccg tgggcagtat cgacgacaat cacgtccacg
     4021 ccggcttcga ccaaggcttt gacgcgctct tcggtatcgc cgccggtgcc gactgccgca
     4081 ccgacgcgca gacggccctc ggagtctttg ttggcgttgg gaaactcggt ggtttttaaa
     4141 atatctttaa cggtaatcag acctttgagt tcgtcttttt cgttcagaac caaaacgcgc
     4201 tcgactttgt gcgtgtgcat cagttcgcgc gcttcgtcta tgcttgtgcc ttcggggacg
     4261 gtaaccagac gttcgcgcgg ggtcataatg gcggaaacgg gcaaatcgac gcggttttca
     4321 aaacgcaggt cgcggttggt tacgatgccg acgactttgc cgttttcaac gacgggcagg
     4381 ccggacattt tgcgtttgcg ctgcgcgcgc atttccaaga cttcgcggat gagcgtcgtc
     4441 ggtgcaacgg ttacggggtc tttgaccacg ccgctttcgt ggcgtttcac tttggaaacg
     4501 gcgcgcgcct gcatctccgg cggcatattt ttatggatga tgccgatgcc gccttcctgt
     4561 gccatcgaaa tggcgaggcg cgcctcggta acagtatcca tcgcggcgga aagcaggggg
     4621 aggttgagcg tgatttcgcg ggtgagcttg gtttgaagtt taacgtctcg cggcagcacg
     4681 gtcgaatgtg cgggaaccaa caaaacatcg tcgaaagtat aggctttttc tacgatacgc
     4741 ataatgctcg gtctttcagt ttgtgcaaga tgcacggcat tatagcacgt taccggcggc
     4801 ttgacagttt atattgtttg caatatcccc cgccttcagg cggcatacgg cgcggcggcg
     4861 gaatgccgtc tgaaggcggg cgttatgata attgttccag cagcgtctgc ttcaatgagg
     4921 actggatttt tgggtttttc aggtctaggc taaaaacggt aaaactgtct tcggcgcgtt
     4981 catccagtgt ggagattttg gcatagcgca ggctgacgtt gtgggcgaaa aagacttccg
     5041 ccatatcggc gagcaggaag gggcggttga cggcggtgat ttcgacggag taccaatcgg
     5101 ggtagtcttc ttcgggggtg atggtgatgc ttggcgcgat cggcatatag cggctgcggc
     5161 ggctgatgcg gcggctatgg ctggagattt cggcaacggt gtgtccgtgg ataaagctgt
     5221 tgagttcggc ttcgagcgcg ctttggatgt cggggtagtc ttcgggagcg tgctgcgagg
     5281 ggatttgcac gatgaaggtg tcgaggatgt agtcgtgttc ggtgataaag gcgcgggcgg
     5341 cgaggatatc gaagccgtgg cggctgaaaa tgcggcagag gcgggcgaac aggcgcgggc
     5401 cgttgggcat gaaaaccatg acttgaaagc tgttgctttt gggcaggatg cggctgcgga
     5461 cgatgggggt ttcaaagtcg tggactaggt tggcggcgtg ccacaggatt tcgcgggact
     5521 ggtggcgggc gaagtaggcg gaaccgagcg cgttccatag ttttttctgc tgtttttcgg
     5581 ggacggcggc gcgggtgagt aagtcggcgg cttcctgccg gcggcggccg aagagggtgt
     5641 gcgggttgcc gccgttgcct gtaaggtagc gtccggcggc atggaagagg ctttccagca
     5701 ggctggcgcg ccatgcgttc cacagcttgg gattggtgcc gcgtatgtcg gaaatggtca
     5761 gaaggtagag cgcgctgagg cgttcgtggg tttggacgcg tttgcagaag gcatcgagta
     5821 cgctggggtc ttggatgtct tctttttggg cgacggtgga cataagaaga tggttttcaa
     5881 ccagccaggc gagcaggtcg ctttcttctt cggtcaggaa gtggtcggcg gcaaattggc
     5941 gcgcgtctgc gatgccttgt atggcatggt cgccgccgcg tcctttggcg atgtcatgga
     6001 aaaaggcggc aaggtagagg atgtcttgtt tttcaaagga ctgcatcagt gcagaggcgt
     6061 agggcagctc gtggctgtgc atatccaagg caaggcggcg gacgttgcgg acgacggtga
     6121 ggatgtggtc gtccacggga tagatgtgga acaggtcgtg ctggagcagg ccgacgattt
     6181 tttcccacgc gggcaggtag cgtcccaata cgccgtagag gttgaggaag cgcagggttt
     6241 gggtcagtcc gttgccgttg cggaaaaagc cggcgaagcg gtggcggttt tcagaatttt
     6301 ggtagaagct gcggttgatt ttgcgcgtcg cgccccacca tgcgcgcagg gtttgcggtt
     6361 cgagcgcggt aatatcgttg cgctgctgca tgatttcgac gattttgaaa atgtgttcgg
     6421 gccgtctgaa aaaaatatcg gtgtgccgcg cggcaatttg gttgttgact tggatgtagt
     6481 cgtcgtcaat ccgcagggta acgcgcaacg gggtggagga aacgcggctt tgcagcatag
     6541 gcgtgaggat gccgcccagt tgtttgacgg ttttaatcgc gcggtaaaac acgcgcatca
     6601 gttcttcgct ttggcggcgg aggttcaagc cttcataacc catgctttcg gcgacttgcg
     6661 gctgcaaatc gaacagcagg cggtcttcgg cgcgcttggc gtttaaatgc agatggatgc
     6721 ggatgtgggc gaggcggcgg tagccgtgcg aaagcatacc ggcttcggca cgcgtcaaaa
     6781 tccgctgttt gagcaggtcg ggcaggtcgg tcgccaagcc ttgcgccttc gctatccaaa
     6841 gcagggtgtg gatgtcgcgc agaccgcccg gacagctttt gatattcggc tccaatactg
     6901 cccccgaacc ttgcgatttg gcgtggcggt gttccatctc caccagtttt gcctcgacaa
     6961 acgccgccac attgcgctgc gcgttcattt ttttcgccag ttcgtccgcc gtttggcggt
     7021 tgccgaacaa aaaacgggcc tctaaaaacg ccgtgtcccc cgtaatatcg tcgcgcacgc
     7081 tttcgcacag ttcgccaatg ccgccgcttt ttacagacgg catcagtttg cagtcccata
     7141 gggtttgaac aaaccgggca atctgttcct gaatgccgtc tgaaagcggg gcaggggaga
     7201 caaccgccaa atccacatcc gaacagggat acagttcgcc gcgcccgaag ccgcccaccg
     7261 ccatcaggca taacgcgctg ttttgaaaat actctgccca caatgccgcc agtaaggttt
     7321 cgactgccgc cgtgtattct ctgaaaaata ccgacacgcg gttggctttc aaataatgcg
     7381 cttcggcggc atcgcgctgc tgtttgaagg tttccagtgc tgaagacagg tttgcaggca
     7441 tttttattct ttcgattggc gggaaaaaag gagacggatg gttcggcggt caaataccgc
     7501 tttcagacgg catttgtcgg gtatcggcgg aattggtcat caggttcaac cgttcctgcg
     7561 gcggcagcag caactgatac gccgccacgc ccaaagccgc cgccattttt tcgatattcg
     7621 acagggcgat gttccagcgt ttgcgttcga ctgccgacac ataagtcctg tccaaaccgc
     7681 attgccgcgc caattcttct tgcgaccaac ccttgttcac gcggaaaagc cgcatattgt
     7741 atgccaatac cgcccgcaaa tcctgttcgt caggcagttc ggcaggcaga gtcaatttgt
     7801 tgcccatcat ttgcttccaa ataaatggtt aaagttaagc atttgctgtt acggatttta
     7861 cttcacataa aagccaaaaa aaaggggtgg cggcagccac acccaaacac acacacatca
     7921 agaggaaaga ggtaacatca agacagattg ggaaacagca aacaagcaat ctagatttca
     7981 tatcggtatt atcaaacaaa aaccgttatc gagtattgat tcaaatcaat ttagatttga
     8041 ttcgcaaacc ggcaaagaaa aacggcatac ccgtttatac ggatatgccg tctgatgatg
     8101 caggggactg ctgtatttgg cagtggtttg tgttttagtc ctcttgcgcc gctgtttgca
     8161 ggtagttcgg caaaccaatt ttgccgatta gttcctgctg ggtttcgagc cagtcgatgt
     8221 gttcttcgtt ggtgtctttt tgtttttcca acaaatcacg gctgacataa tcctgttgcg
     8281 cttctgctgt ggcgatggcg gcaagcaggg cttcgtgttt ttcctgttct ttggtcaaat
     8341 cgcaggcaat gatttcttcg gtggactcgc caatcagaag cttgcccagt tcttgcaggt
     8401 tcggcaggcc ttcgaggaac agaatgcgct cgatcaaatc gtcagcagct ttcatttcaa
     8461 cgatggactg tttgaagaaa tgttcgccca gttcttcaaa gccccagttt ttcaaaatac
     8521 gggcgtgaag gaaatattgg ttgatggtta ccagcagcaa gcctaagttt ttgtttagct
     8581 cgcggataac cagacggtcg cctttcatac ggactccttt tattccgaat acggattaca
     8641 gttggctttg gtagtagttg ccttcgccaa ttaactcgat caggcgaagc tgctgttcca
     8701 gccagtgggc gtggtcttct tcggtatctt tcagttgggc aaccatcagg tcgcgcgtaa
     8761 catagtcttg agcctcttcg cacagtttga tgcctttttt caacgcatca cgtacttcat
     8821 attcggtttg caggtcggct ttgaggcagg aaaccacgtc cgtgccgata ttcagttcgg
     8881 cgcgtgccat tttcggcgtg ccgcccagca tcaggatgcg gcggatgaag tcttcggcgt
     8941 gtgtggtttc ttcttccatc tcgtgattga gacgttcaaa aagtttggtg tagccccatt
     9001 cggagtagag gcgggagtgg ataaagtatt ggtcgcgtgc cgccagctcg ccagacagca
     9061 attcgttcat ataatcaaca acagcttgat tgccttgcat aatatctctc tttccgtaac
     9121 ttggttttcg gtatgtctga acagcatctg tgtcgctatt cgggatgcgt gcattgtatg
     9181 caaaagtttt tgatatgaca aattttctat ttaaaataca aacaattatc aaaagaaata
     9241 gggcgtattc cccgatgcct tccaaatcag tatgctgttt cttatcggtt tttttgctgc
     9301 taaaactaag aatccatctc atccataaag aacatttaca cttattaacc atagcaaaaa
     9361 acaaatagag cacggttttt tatcaaaatt tataatgaat cgttctcatt aaccgacaga
     9421 ttcttttgag attatcggat tttgggaata aattatgccg tctgaggggc tttcagacgg
     9481 catagacaat taaagctgat taaacaacgc ggcagacagc agcgtaggaa attatttttt
     9541 acttcggaac ggtattttgg gtttaatgat gcccgcaacc gccgtggctt tcgcgtaagg
     9601 cttcggcagc ctgttcgtcg gcgtggtagc tggaacgtac catcgcgccg atggcggcat
     9661 tgctgaagcc cagttcgtat gcttcttttt caaagatttt gaactgctcg ggcgtaacgt
     9721 agcgcaggac gggcaggtgt ccgtctgaag gctggaggta ctgtccgatg gtaatcattt
     9781 cgatattgtg cgcccgcata tcgcgcataa tttcacgcac gtcttcgtct gtttcgccca
     9841 agccgaccat gatgccggat ttggtcggga tgtgcggcat catttcttta taacgtttta
     9901 ataagtctaa agaatgttga taattggcac cgggacgggc ttttctgtac aggctcggat
     9961 gggtttctag gttgtggttc atcacgtcgg gcggggtttc ggcaaggatt ttgagtgcga
    10021 tgtccaagcg tcctcggaag tcggggacga ggatttcgat tttggtgttc gggctggttt
    10081 cgcggatggc tttgatgcag tcggcgaaat gctgtgcgcc gccgtcgcgc aggtcgtcgc
    10141 ggtcgacgga ggtgatgacg acgtaacgca ggttcatggc tttgacggat tcggcgaggt
    10201 ttttcggttc gtcggggtcg agcatattcg ggcggccgtg tcccacgtcg cagaacgggc
    10261 agcggcgggt gcagatgtcg cccataatca tgaaggtcgc cgtgcctttg ctgaagcatt
    10321 cgccgatgtt ggggcaggag gcttcctcgc aaacggtgtg cattttttgt tcgcgcaaaa
    10381 tgtctttgat ttcaaagaat ttgcgcgatg gaagtttggc gcgtatccat tcgggctttt
    10441 ttaatttttc ctgaaggggg acgactttga tggggatgcg ggcggttttg tccgcgcctc
    10501 tgagtttgat gccgcgtttg gggtcgtcgg ttttgatttc actcattgtt gtctgctttc
    10561 ggtgtgaatt gtgtttcaag gtgtgcggtg agtttggcgg cgacttcgtc cggcgtgggg
    10621 cagggttgga caaaatccgc gatttgcgtc atttccatac cggcgtagcc gcaggggttg
    10681 atgtgggtaa acgggcttaa atccatattg acgttgagcg caagcccgtg atagacggag
    10741 ccgtttttga tacgcagccc cagtgaggcg attttgcgtt ctccgacata aacgccgggg
    10801 cgtttggggt ctgccgccgc ttcgatgccg tattctgcca atgtggcgat gatgctgttt
    10861 tcaagcgcgg aaacgatgtt gcggacgctg gttttgcgcc gtttgaaatc aatcatcgta
    10921 taaacgacca attgcccggg cccgtgatag gtaatctgcc cgccccggtc gatttggacg
    10981 acgggaatgt cgtcgcgaat cagcaggtgt tcgggttttc ccgccagtcc ttgtgtgaac
    11041 acgggcgggt gttcgacgac ccacagttcg tcttcggtgt cggcattccg tccggcatta
    11101 aaggttttca tcgcttcaaa agtcggcaga tattcgacca aacctttgtg tatgattttc
    11161 atctcaaagt accactttga ccagttcgtg cgaagtcagc gcacggtaga tgttgtccaa
    11221 ttgttcttgg ttttcaacct ttacctgtac ggtggcgcca gtatagttgc ctttgctgct
    11281 cggacgcgtg gtgatgtggt gcgcctgcgt gtcgggggcg tgaaggcgga cggtgtccaa
    11341 taccgcctgc tcgaactcgg gatgcaccgc gcccattact ttcaatggga aggtgcaggg
    11401 aaattcgatg agggatgttt tgttttcttg ttcggtcatg atgtgctgcc ttgtcgtgta
    11461 cggtatgccg tctgaaggcg ggtttgcctt tcagacggca tcggatgtgc gttattttag
    11521 cctaaaccgc gataacaggc tatcgggaag gcggaggctt ttttgacggc gcggcggttc
    11581 tgctatactg gcgcacaata ttatttttag aagggtggtt tttatgtatc ggaggaaagg
    11641 gcggggcatc aagccgtgga tgggtgccgg tgcggcgttt gccgccttgg tctggctggt
    11701 tttcgcgctc ggcgatactt tgactccgtt tgcggttgcg gcggtgctgg cgtatgtatt
    11761 ggaccctttg gtcgaatggt tgcagaaaaa gggtttgaac cgtgcatccg cttcgatgtc
    11821 tgtgatggtg ttttccttga ttttgttgtt tgcattattg ttgattattg tccctatgct
    11881 ggtcgggcag ttcaacaatt tggcatcgcg cctgccccaa ttaatcggtt ttatgcagaa
    11941 cacgctgctg ccgtggttga aaaatacaat cggcggatat gtggaaatcg atcaggcatc
    12001 tattattgcg tggcttcagg cgcatacggg cgagttgagc aacgcgctta aggcgtggtt
    12061 tcccgttttg atgaggcagg gcggcaatat tgtcagcagt atcggcaacc tgctgctgct
    12121 tcccttgctg ctttactatt tcctgctgga ttggcagcgg tggtcgtgcg gcattgccaa
    12181 actggttccg aggcgttttg ccggtgctta tacgcgcatt acaggcaatt tgaacgaggt
    12241 attgggcgaa tttttgcgcg ggcagcttct ggtgatgctg attatgggtt tggtttacgg
    12301 cttggggttg gtgctggtcg ggctggattc ggggtttgca atcggtatgg ttgccggtat
    12361 tttggttttt gttccctatt tgggcgcgtt tacaggactg ctgctggcaa ccgtcgccgc
    12421 cttgctccag ttcggttcgt ggaacggcat cttggctgtt tgggcggttt ttgccgtagg
    12481 acagtttctc gaaagttttt tcattacgcc gaaaatcgtg ggagaccgta tcggcctgtc
    12541 gccgttttgg gttatctttt cgctgatggc gttcgggcag ctgatgggct ttgtcggaat
    12601 gttggccgga ttgcctttgg ccgccgtaac cttggtcttg cttcgcgagg gcgtgcagaa
    12661 atattttgcc ggcagttttt accggggcag gtaggcggtt ccgaaacata tttgaagcgg
    12721 aatacaacct tgcccgggtt taaataaaaa tgccgtctga accccgaaaa cgggacttca
    12781 gacggcattt tcatcacggc ttatttggcg gttttgctgc tgtcgataat tttcataccg
    12841 gcagaaatca ggctgccgat gtcggcaaca ttggcgggca taatcagcgt attgctttct
    12901 ttggcaagat tgttgaacgc ggcgacgtat tgttccgcaa tcttcagatt gaccgcatcc
    12961 gcaccgcctt gggtttgaag ggcggcggca atttgacgga tggcttcggc attggcttcg
    13021 gcaacaaggc gcaaggattc cgcttcacct ttggcgcggt tgatgcgggc gattttctcg
    13081 gcatttgacg cattgaccgc agcctgagcc tcgccttcgg attgttggat ttcggcttcg
    13141 cgctgaccac tggcaaggtt gatttgttcg attttacgac cttcggattc ggcgatacgg
    13201 gcgcgttttt cgcgttcagc agtaatttgc gcctgcattg agcgaaggat ttcttgcggc
    13261 ggaaccaagt ctttaatctc ataacgcaaa accttcacac cccaagctcc ggcggcttca
    13321 tcgagggcgg agacgacggt gctgttgatt tcgtcgcgtt cttcaaacgt tttgtccaat
    13381 tccatacgcc cgataacgga acgcagcgtc gtttgggcaa gctgggtaat cgccataatg
    13441 tagttgctcg aaccgtatga ggcgagtttg gggtcggtta cttggaaata gatgataccg
    13501 tcaacagtca gctgcgtatt gtcgcgcgtg atgcagacct ggctgggtac gtctaaaggg
    13561 atttctttca gcgaatggcg gtaggcgacg cggtcgataa agggaatcaa aatattcaaa
    13621 ccggccgtca gggcgcgatg gaaacgcccg agcctttcga caacgtggac ttcctgctgt
    13681 gggatgacaa caaaggattt gaagccgaaa acaacgacgg ctgccagcaa gataatgaaa
    13741 aattccataa ttcctccgag tgttaaggtt ttgcgatgat aagaaggttg ccttccttgc
    13801 ggacgattag ggcgcgcgtt cctggttcaa gctcttcttg ccccgtattt tgagcctgcc
    13861 agtgcgtacc gcgataaaaa acttcgtaac ggttgccgcc tgcgtgccgg aggatttcgg
    13921 catattgccc ggcatccaaa tcctgatatg aatccgtttc aacttttccc acggcggttt
    13981 tggcgtgtac gaaccaaata cccagcgcgg aaagcagagc ggcggtcaag acggcggcag
    14041 gcgtgctgcc ggtcagcccg taagcaatgc ccgaacccgc caaagccgcg ctgacaacca
    14101 aaagataaac cgttcccgtc aataattcga tgattaagac ggcaacagcg gcaacaaacc
    14161 atacagtcat acatttcccc acaaagcgcg tcgtttgaca aaatcacgcg atatcagcag
    14221 tatagccgaa tttgaaagga tagggcagat atggacactt ggcacgatgc actcggcggc
    14281 gaaaaacagc agccgtattt tcaggaaatt ttaaatgcag tcaggcagga acgtttgtcg
    14341 ggacaaatca tctatccgcc ggcggcggat gtgttcaacg cattccgcct gacagcgttc
    14401 gaccgggtca aagccgtcat tctcggacaa gatccgtatc acggggcagg gcaggcgcac
    14461 ggtttggcat tttccgtcag gcagggcgtc cgcatacccc cgtctttact caatatctac
    14521 aaagaattgg aaaccgacat cgaaggcttt tccattcccg cgcacggctg cctgacagcg
    14581 tgggcggagc agggcgtatt acttctgaac acggttttga cggtgcgtgc aggacaggcg
    14641 cattcgcacg cccttttagg ctgggaacgc tttaccgata ccgtcatcag gcagcttgcg
    14701 acacaccgca agcatcttgt ctttatgctg tggggtgggt atgcacaaca aaaaaggaaa
    14761 ctgatagaca gtcaaaacca tttgatattg accgcaccgc atccgtctcc tctgtcggca
    14821 tatcgcggtt ttttcggttg ccgccatttt tcacaggcaa acagctattt gagccggcac
    14881 ggtatcgatc cgataaactg gaagctgtga atgccgatat agccgttgcc gccggcgtgt
    14941 taaaatcgcg tttgatttgt aatttccatt tattaggcaa aaccttatgg caatcaaaaa
    15001 aaaatcagct ttatgcatca ctttgggcgg gctgcgacga gttgcgcggc ggtatggatg
    15061 cgagccttta taaagaacta tgtgcttacg ctattgtttt taaaatatgt ttctgataag
    15121 cataagtacg gcggcggcat gattgagctg cacgccggta cgacttttga cgacatcgtc
    15181 aaactcaaaa acaccgccga catcggcgac cgcctgaata agattatcgc ccaaattgcc
    15241 gaagccaacg acttaaaagg cgtgatcgac gttaccgact tcaacgacga agacaaactg
    15301 ggtaaaggta aggagatgat cgaccgtttg agcaggcttg tcggcatttt taaaaagctc
    15361 aacctttctt ccaaccaagc cgaagacgac gatttgttag gtgatgccta cgaatacctg
    15421 atgcgccatt ttgcgaccga gtcaggcaaa tccaaagggc agttttacac gcctgccgaa
    15481 gtctcccgca ttatggcgaa gattatcgga atcagcgcag attgccgtcc agcaccagcg
    15541 tttatgaccc gacctgcggc tcgggttcgc tgttgctcaa agccgccgcc caagccggca
    15601 gccaaatcag cctttacggt caggaaaaag atgtggcaac cgcgtccctt gcccgtatga
    15661 atatgatttt gcacaacaac gaaaccgccg aaatcaacac cgggaacacc ttgtccgatt
    15721 cgtctttccg tgatgaaaac gacgggctta agaccttcga ttttgccgtt gccaatccgc
    15781 cttatcccgc ccgaaaaaaa cggcgattac gcctttttgc tgcatctgct caaaagcctg
    15841 aaaccaagcg gcaaaggtgc gattattctt ccgcacggtg tgctgtttcg cggcaatgcc
    15901 gaagcgcgta ttcgcacgga attgcttaac cttgacctta ttaaaggcat tatcgggctg
    15961 cctgccaacc tgttttacgg cacgggcatt cctgcctgca tcatcgtcat cgacaaagaa
    16021 cacgcccaaa ccgcccaatt tgccgaagag ggaacaaacc aagttatcag cggcggcagc
    16081 gtgtttatga ttgacgcatc gcgcggcttc attaaagacg gcaacaaaaa ccgtctgcgt
    16141 gagcaagaca ttcacaaaat catcgacact ttcacaaacc tcgttacagc cgtatggtgc
    16201 atttaagcga aatcgcagca caagattaca accttaatct gcctcgctat atcgacagcg
    16261 gagaagtcga agacctgcaa aatctcgccg cctatctatc tttatggcgg catacctgcg
    16321 cacgatatgg acgcattgga agcctattgg caagttttag gccgtatgaa aaacgagttg
    16381 tttgccgaac acgatggcca ctttaccact atacaacgga atcgattgca aatctttccc
    16441 actctcaaca gcttaaaatc ctgcgggatt ggtgtggaat ttagggctaa tctagtacag
    16501 ccccaaattt aatccactat aaaatcgaaa gcagccaaat caaagcccat atattggcgc
    16561 accccgatta cgccgccttc aaagccggac acctggcaaa gtttgccgcg tggcacactc
    16621 aaaacgacct tgccgccatc caaccgggca ggcttatccg gaaatggagc gaaagcctgc
    16681 tggacgcgtt caaacccggc agcctgattg aagaatacga tttctaccaa atcctgacgg
    16741 actactgggc ggaaaccctg caagacgatg tttatctcat cgcccaagat ggctggaagg
    16801 cggttaaaaa cctggccgaa atcaccaaag aaagcgatga agccgcgaac ctgaccgtcg
    16861 tctttgagga aaccgaaacc gacaaaaaag gcaaagccaa aaccaagcgc atcagcaaaa
    16921 aataccgcag cgaagtcatc gcccccgagc tggttgcccg ccgctacttt tcagacggca
    16981 tcgccaagct ggaagaaaaa caaagcgagc tggaacgcct aagccaagaa ttggaaaacc
    17041 acatagaaga acacggcggc gaagagggtg cgctgaacga cgtattggat gcaaaaggca
    17101 aactttccgc caaacttctg aaaaccgcat tggaagaaag cggcatagaa gaaggcgaac
    17161 gggctgtttt acaaaccacc caaacactga tgacgcagga aaaagccgcg aaagacgcag
    17221 tcaaaaccca aatcgaagcc ctgaaccttg ccgtattcaa acaattcggc cgactttccg
    17281 aagccgaaat caagcagctt gccgttcaag acaaatggct tgccgattta caaagtcgaa
    17341 tcgaaaatcg cttggaaaac agtattcagc agcttatcag ccgcttgaac acgctggaag
    17401 accgctaccg cagcccgatg gccgagcttg cccgagaagt ggaaaagtgg caaagcaaag
    17461 tcaatgccca ccttgaaaat atgggttttg gaggctgaaa tggcggcaca gacaggctat
    17521 aaggcgagcg ggttttgaga cctttgcaaa attccccaaa atcccctaaa ttcccaccaa
    17581 gacatttagg ggatcgcggt tcgggtgtcc gcaccgctta atacgtcgtc gtccacgaac
    17641 tgacccattt gctcgaacgc catcgcaacg cccgttttat ggcgcatatg gacaactttc
    17701 tcccaaactg gcaaagcatc aaacaacagc ttaatgcctt ggagttattt gcacaaatat
    17761 ataatttaac ataatataca ttatgcgaac tatcggaaac agttgtacgt gtccctgtgg
    17821 tctttccaag taggaaaatt aaagtatgga caatgcggct gaatgtatag cccggagcat
    17881 ccgcgtatcc gaaggtactg aacgatatgc cctgcatccc tttcgatgct tccatccagc
    17941 cggtattggg aaatacgttg accttcaata taatatacag gcagccgaca cacaggatat
    18001 gccgctgctt tttcatcatt tcttctgtca aatccttgga acggtcgatt tgaaaacacg
    18061 gctttacata cgtcccaatt ctctttgcag tgcttggata ttttgctgcc tgtccaatac
    18121 attgctttgt aatgcattta tttcttgatg gttgatgttg ccgccctttg ccagacgtgc
    18181 ttgtgataac attttttggg cttcagtcag ggctttgcgt tcgttgctca attctgcttc
    18241 gagaatggag cgtctgctgt tgtttacagg tgcttgttga ggcgggggcg tattggattt
    18301 tgctggcttg gatactgttt tgaccggggc tttatatttg acaacctgtc cgccgtttga
    18361 cggtgatgat accggttcgg gcgtttgggg cgggatatag cgttcgctgc tgtagttgcc
    18421 gattgggggc aaatcggttg agtggcagct tttggacggc ttggtggtgt aaacggtttc
    18481 tccgttgatt gtgcaggtgt agattttggc cgcattcgca cccaatgggc ttgaaatcag
    18541 ggaaaagttg attaggatta aggggagttt tgatttcata atgtgcttga tgctcggata
    18601 attatttgat tttttggtat tttttataat gctttcttgg cgttttacgg gcttttcttt
    18661 tcggttgttt gcttttgatt ttctttcggg gctttggcag cctgcttgtt ctttttcccg
    18721 gcaggttttt tgtttttctt tttactttct atgtgtattt tggcttctta actgagtttt
    18781 ttaattttca ggcggtatcc gcctccctga tggctgctga ttttaggtaa atccgcatcg
    18841 gcgcacgatc ctgctggggc tgatgtatat acgcttttgc catttgagtt gcaatggtat
    18901 acggaggctt gcgccgccgc gccggaaagg gacaggagac ccaaggcggc aaaaagtctg
    18961 atgtttatac cggtaaaagg cggtgataac gcccgaatta taccgttatt ggcaggcaaa
    19021 gataagcacc ctgtccgcgc ttctttatcg ctcggcaaac tgtttctcga tttcagtttc
    19081 aatccgttcc aattcttctt gacaagccag ccaaacctct tcgatttggg caagttgtgt
    19141 tttgactttt gtcagcaggg ataaggtatt ttgcaatttt tctttgtttg cttcaaggta
    19201 agcgtcttct tgtgctaaaa atgcttcaca tgccgtctga atttcggaaa gctgcgccat
    19261 ttctttttcg gcacggtcta ttttctgctg tatcggcttg ccgcgtcggg ctttttcctg
    19321 acggatttgc gcttcgatgc gcttggtgtc tttgcggctt tggctttgtg cggatgctgc
    19381 gggtgcggcg gcggcgtttt cctgtgccaa acgccattgg cggtaatcgt tcaaatcgcc
    19441 gtcgaagttc ttcagacggc ctttgtcgat caggaggaag ctgtcggtag tcgcttcaag
    19501 caggctgcgg tcgtgcgata cggcgattaa tgcgccttgg aaactttgca acgcgagcgt
    19561 taaggcgtgg cgcatatcca agtccaaatg gttggtcggt tcgtcaagca gcaacaggtt
    19621 cggcttttgc cagataatca tggcaagggc aaggcgggct ttttctccgc cggaaaaagg
    19681 ttcggttttc tgtaacgcca tatcgccaac aaaattgaag ccgccgagga aatttcggat
    19741 ttcctgttcg cgcacttcgg gcgaaagctg ctgaatatgc caaacagggt tttggtcggc
    19801 gcggatggta tcgagttggt gttgggcaaa atagccgata ttgagttttt cggaacggat
    19861 gatgctgccg gaaagcaaat cgattttgcc tgccaaggct ttgataaagg tggatttgcc
    19921 gctgccgttg acacccaata gcccgtagcg tgcgccgctt tccagcgaca gggtaatgtc
    19981 atgcagaacg gttttgcctt cgtaacccaa atcagcgtgt tctagcttta acaaaggatt
    20041 gggcagatgg tcgggatggt aaaactcaaa ggaaaactcg ctgtccagat gcgcgggcgc
    20101 aacgcgttct agctttgcca aagccttcat gcggctttgc gcttgaacgg ctttggtggc
    20161 tttggctttg aagcggtcga taaaggattg cagatgtttg atttgcgcct gctgtttgac
    20221 ataggcagct tgttgttgcg cgagacgctg cgcgcgttcg ttttggtaaa aatcgtaatt
    20281 gccgccgtat tgcgtgagct tttgctggga caattcgatg gtttgggtgg ttgccgcatt
    20341 gaggaaatcg cggtcgtggg aaatgatgat ttgcgtgcag ggtaaagagg caaggtggtt
    20401 ttccagccac aagacggttt ccaaatccaa gtggttggtc ggttcgtcaa gcaagagcaa
    20461 atcggcgcgg caaatcaggg cttgcgcaag attcaggcgc atacgccagc cgccggaaaa
    20521 ggatttgacg ggacggctgt gttcttcttg cgaaaaaccc agcccgttca acaatttcgc
    20581 tgcacgggcc ggcgcggtat aagcgtcgat ttcttccaat ttagcatgat attccgcctg
    20641 cttcatgccg tcattttgcg cttccgcctg cctcaatgcc gtctgaaaag cctgcaactc
    20701 ggcatcgccc tgcaaaacgt aatccaaagc ggaaatatcc aaatcgggcg tttcttggga
    20761 aacggaagcg agccgccagt ttttcggaat cgagacatcg ccgccgtcct gagtgatttt
    20821 acccttgatt aaggcaaaca ggctcgattt gcccgttccg tttttgccga tcaaaccgac
    20881 gcgctgaccg ggattgacgg tagcgttggc tttgtcgagc aggactttca aaccgcgttg
    20941 cagggtgagg tttttgattt caatcataac ggaaacatcg tcgggcggaa aaagcccgta
    21001 ttttacctga aagtcagtgc cgatgccgtc tgaaatggga aatttacggc tgaagccaag
    21061 cccaagccct gcgcccttcc gagtgcagga aaaccaatgt cctgaatgcc gaatcggtat
    21121 tcatgcattc cacgctgatt ccgattcggg aaaaatccgc catgattttg ggatggataa
    21181 actcctgagc cgcgcccgtc ccgataatca atatttccgg atagtcaaca ggtttgatgt
    21241 cggacaacag gttttccgga gtcagatcgg acaaggttcg gcattgcgac aggcagaccg
    21301 aatccttatg tacaagcacg ggtttatgga aactttgccc cgtcagccgg attccgcccg
    21361 caccgcattc atattccgca aactgtccgt ctatcggatt ttcttcaaac aacatttttt
    21421 taccccgtcg ccgcatcatc tacaccgaaa ggggtgcaaa atcagacaaa ttcatgtagg
    21481 attggcagat ttcatctgac ccgcctgccg atttcagacg gcatttgatt caaagtgcgg
    21541 cacaattata tcggcagcgg atattttcgt ctttcaatat ttacatttca gtcggcttac
    21601 aaggagacac aatgaagcca gtaaacatcg gtcttttagg tttgggtacg gtcggcggcg
    21661 gtacggctgc cgtgttgcgg gacaacgcgg aggaaatttc ccgccgcttg gggcgcgaaa
    21721 tccgtatttc tgccgtgtgc gatttgagtg aagaaaaagc ccgacaaacc tgcccgtccg
    21781 cagcctttgt caaagatccg ttcgaactgg tcgcacgtga agacgtcgat gtcgtcgtcg
    21841 aattgttcgg cggtaccggc attgccaaag atgcggtgtt gaaagccatt gaaaacggca
    21901 aacacatcgt taccgccaac aaaaaactgc tcgccgaata cggcaacgaa atcttcccgc
    21961 tggcggaaaa acaaaacgtc atcgtccaat ttgaagcggc agtagcgggc ggtatcccaa
    22021 tcatcaaagc cctgcgcgaa ggtttggcgg caaacaggat taaatccatc gccggcatta
    22081 ttaacggcac cagcaacttc atcctctccg aaatgcgcga aaaaggcagc gcgtttgccg
    22141 atgtactgaa agaagcgcag gcattgggtt atgccgaagc cgatccgacc ttcgacatcg
    22201 aaggcaacga tgcgggccat aaaatcacca tcatgagcgc actggcattc ggcacgccga
    22261 tgaacttttc cgcctgctac ctcgaaggca tcagcaaact cgacagccgc gacatcaaat
    22321 acgccgaaga attcggctac cgcatcaaac ttctgggcat cacccgcaaa accggcaaag
    22381 gcatcgagct gcgcgtccac cctaccctga ttcccgaaag ccgcctcttg gcaaacgtca
    22441 acggcgtgat gaacgccgtg cgcgtcaacg ccgatatggt tggcgaaacc ttatattacg
    22501 gcgcgggcgc gggcgcattg ccgaccgctt ccgccgtggt tgccgatatc atcgacatcg
    22561 cccgcctggt tgaagccgat accgcccacc gcgtaccgca tctggcgttc caacccgcgc
    22621 aagtccaagc gcaaaccatc ctgcctatgg acgaaattac cagcagctac tacctgcgcg
    22681 tccaagccaa agacgaaccg ggcacgctgg ggcaaatcgc cgcgctgttg gcacaagaaa
    22741 acgtgtccat cgaagcactg attcaaaaag gcgtgattga tcagaccact gccgaaatcg
    22801 tgattctgac ccacagcacg gtcgaaaaac acatcaagtc ggcaatcgca gccatcgaag
    22861 cactggattg cgtggaaaaa ccgattacca tgatccgcat ggaaagcctg catgactgag
    22921 ccgaaacacg aaatgccgac ggaagagcag gttgccgcgc gcaaaaaagc aaaagccaaa
    22981 atccgcacca tccgcatttg ggcgtgggtc attttggcgt tgctcgcttt aaccgccctg
    23041 ctctcccaat gcgcgatgtc caaaccgcag gcaaaacaga aaattgtcga gtcttgcgtg
    23101 aagaatattc cgtttgccga aaaatggcaa aacgatttgc gggcccgcgg tttagattca
    23161 aacaataccc gcctcgccgt cgactactgc aaatgtatgt gggagcagcc tttggacaga
    23221 ttgagcgaga aacagattag atccttcggc aaactcggcg cacaagaaca gcttgacctg
    23281 ctcggcggcg caaatgcctt tgaagcacgt gacaagcagt gtgttgccga tttgaaatca
    23341 gaataatgtg gaccgataaa aaagccgatt ctttaaagaa tcggcttttt tcataaaaaa
    23401 cggcttacag tgcgtctttc aatgctttac ctgcacggaa tttaggcgtt ttggcggcgg
    23461 caatggtcag aggctcgccg gttttggggt tgcggccttg gcgttccgca cgttcgccca
    23521 cgtagaaagt accgaaaccg accaaagtaa cggtgtcgcc ttgtttcagg gcggtggtta
    23581 ctgcattggt agtggcatcc aaagcttttt gtgcggcggc tttggaaatg tcggcttctt
    23641 gagcaatcgc ttcgatcaat tcagacttgt tcacaatcag tcccttcctg tcttaaaaaa
    23701 tgatgaaatg cccgaatact cggggtttgt actgcttgag caactttcgc tttatagcaa
    23761 ttctgaaatt gccgtgtcaa gcaaaaaata cggaatcacc ctatttgaca ggctttcagg
    23821 acgaaaccgc atttttacaa cacatttcct gcgtttcaat gtttggttgc cctgctgcgg
    23881 ggttttggtt ttgaagcgga ttccgccgcc gcttccgcac cagaaggttc tgcccaaggc
    23941 tcaggctggc tttccaaacc cagagccaat acctcgtcta tccatttgac cggatggatg
    24001 gtcaggccgg ttttcacgtt ttcagggatt tcttccaagt ctttgacgtt gtctttcgga
    24061 atcaggacgt gtttgatgcc gccgcgcaag gcggccaaca gtttttcctt cagaccgcca
    24121 atcggcagga cttcgccgcg cagggtaatt tcgcccgtca tcgccacatc ggcgcgtacc
    24181 gggattttgg aaaatgcaga caccatcgcc aaagtcatgg caatgcccgc actcggaccg
    24241 tccttcggcg ttgcaccttc gggaacatgg acatggatgt cttttttctc gtaaaaatca
    24301 gaagccaagc ccactgattc cgcgcgggag cggacaaccg accacgctgc agacacggat
    24361 tccttcatca catcgcccaa ctggccggtg cactgaatca cgcccttacc cggcaatgct
    24421 gcggcttcga cggtcagcaa ttcgccgccg acttccgtcc acgccaaacc ggtaacctgc
    24481 ccgatacggt tttcgctttc ggcaacgccg taatcgaagc ggcgcacacc caaatagtcg
    24541 tgcagatttt tctcatttac tttaaccgct ttaggtttgg ctttgctggt tttcttggtt
    24601 tcagacaacc tcttcttatc ttcgtccaag gtaatctgca tcaccacctt gcggcagatt
    24661 ttggcaatct cgcggtcgag cgaacgcacg cccgcctcgc gggtgtagta acggataata
    24721 tcgcgcaccg cgctttcttc gacgagcaat tcgccttctt tcacgccgtt gcgcttcatt
    24781 tgcttcggca cgaggtactg catcgcgata ttgatttttt cgtcttcggt gtagccggac
    24841 agacggatga tttccatacg gtcgagcagc ggggtcggga tattcaggct gtttgaagtc
    24901 gcaataaaca tcacatcgct caaatcgtaa tccacttcgg cataatgatc ggcgaacttg
    24961 ttgttttgct cgggatcaag cacttcgagc aacgcgctgg cgggatcgcc ccggaagtcg
    25021 ctacccaatt tgtcgatttc gtcgagcagg aacaatgggt tttttacgcc ggcttttgcc
    25081 atattctgca gaatcttacc aggcatggag ccgatatagg tgcggcggtg tccccggatt
    25141 tcgctttcgt cgcgcacgcc gcccaaagcc atgcggacat atttccgccc cgttgctttg
    25201 gcgatggatt cgcccaaaga ggttttgccc acgcctggag ggccgaccag gcacagaatc
    25261 gggcctttga gtttgtccat acgtttttgg acggcgaggt attccaaaat ccgttctttg
    25321 actttttcca ggccgtagtg gtcggtatcc agcaccaatc cggctttggc gatgtctttg
    25381 ctgacgcggg attttttctt ccacggcagt tcgagcaggg tgtcgatgta gttgcgtacg
    25441 acggtggatt cggcagacat cggcggcatc attttgagtt ttttcagttc ggacaggcat
    25501 ttttcttccg cttctttgct catccccgcc tttttgatgc ctgcttccaa ggcatccagt
    25561 tcgccgtttt cgtcttcttc gcccaattct ttgtgtatcg ctttaatctg ttcgttcaga
    25621 taatattcgc gctgggattt ttccatttgg cgtttgacgc gtccgcgtat gcgtttttcg
    25681 gcctgcataa tgtcgagttc ggattccagc tgtgccagca ggaattccat ccgtttgccg
    25741 atttcgggaa tttccaaaat ctgttggcgt tgcgccagtt tcaactgcaa atgcgctgcg
    25801 accgtatcgg ttagccggct gttttcggca atgccgttaa tgctgccgat aatttcggcg
    25861 gggatttttt tattgagttt ggcgtattgt tcaaactgcg ccaacagggt gcggcgcacg
    25921 gcttcgaggt cggtattgcc gcccgtgtct tcttccacga ccgcctctat atgggaaacg
    25981 aacagaccgc ccgtgtcttc aatggtcaga acacgtccgc gatacagccc ttcgaccaat
    26041 acttttaccg tgccgtcggg cagtttcaac acttgcagga cttgtgcgac cgtaccggtc
    26101 tgatacaggt cggcggcaac cggttcttct accgccgcat cggtttgcgc caacaggaaa
    26161 accggctcct cgcgggtaat ggcgttttcc agtgcggcga tggatttcgg cctgccgaca
    26221 aacagcggca gaaccatatg cgggtaaacg acgacatccc gcaaaggaag ggttgccaag
    26281 gcggcatatt cctcaaaatg cttttctttt tgtgtcatag gtactctctt gtgtctgaca
    26341 gattgccgat tttcgcgtac attggggttg aaggtattat ttcaagcata tgtggtttat
    26401 ttatggagtt tgatgcgatg ccgtctgaaa cattccggct tcagacggca tgggcttgga
    26461 aagacaaggc gggaacaaaa aactgttctg tgttgccgct ccttgctgta ccatccgtat
    26521 ggtttgcggt tctgccgccc tattttcaaa acatgacgca ggtattccat gtcttctttt
    26581 atttttcccg ataacgggtt cgaatggcgc aacgaaagtc tgcaaaagcc gcccaaaggt
    26641 tggcattatg tccgcgaaag cggtgcaaac ggcattttga aggctgccta tcaaaatatt
    26701 gcaactgtct ggcagggcga tttccacaat gccaaacagg tgctttccgc aatgaagaag
    26761 agggttctca aacctgccgc cgtccgttcc gatgcggata tcgccgccct tttccacgcc
    26821 caccgtatga agcaggcgca gcagagccgt attctgaata tgcttgccgt tgaaatccgc
    26881 cccggttttg tgttggacaa caaacgcgcg cccgatatac gcgccgccct gctcgacgtg
    26941 tacggagagg cggacggcaa accgtttttc ctgccgctca atctgctgct ggggtttatg
    27001 ggtgcgcacg agtggcataa gaaaggggtt gccgttccgc agctgggcgg cagcatacac
    27061 gttcctttcg gcgtattctc gccgttgcgc ggcgaatacc tcgacctgct cgcccatgcg
    27121 ccgtcaacgg gttttcagac ggcattagat atcgggacgg gctcgggcgt gcttgccgcc
    27181 attttggcga aacagggcat tccttccgtc atcggcacgg ataccaatcc gaaagccgtc
    27241 gcctgcgccc gtgccaatat tgcccgtttg ggctttgaaa aacaggttga gatccgcgaa
    27301 accgatctgt ttcccgaagg gtttgtcgat ctgatcgtct gcaatccgcc ctggcttccc
    27361 gccaagccga cttccgccgt cgaatccgcg ttatacgacc ccgaatctgc gatgctggct
    27421 gcgtttttgc gggatgcgcc gaaacatctg aatcccgacg gagaaatccg cctgatcatt
    27481 tccgatcttg ccgaacatct gcacctgcgt ccgtccgatt ttctggataa ggcatttgct
    27541 caggcgggtt tgcgcgttgc ggatgttttg aaaaccaaac cggttcacaa aaaagctgcc
    27601 gatccgaaca atccgctggc ttttgcccga aacagggaaa ctactttcct gtaccgtttg
    27661 aaaaaggcat aaggggcggc ggcgcggcat tcgggcggat tattcttgtg aaaatacccg
    27721 ctcgagcata ctgcccaatg ccgtctgacg cgttttgaca gtggcggcag cttgccggaa
    27781 ggtttcttct ccgccgaaga atacgaactt ctccctcgcg cgggtaatgg cggtatataa
    27841 cagctcctta ctcaatccgg acaatgcatc gtccccttcg tccgaaggtg cggcggaagg
    27901 cggcagcagc cagacttccc gatattccga accttggctt ttgtggacgg tcatggcgaa
    27961 tgcgggttca aattcgggca ggcagcttac cgctaccttt ttaaatccgt ccgcatcggc
    28021 aaaataggcg gcaaggctgc cctgccgtcc gacatcttcc ataatcagtc cgatgtcgcc
    28081 gttgaacagt tcaagcgcgt agtcgttctg tctgatcata atcggttctc cggcgaaata
    28141 ggcctgatgt tccggtatgt tcattttgcg gcgtacatgg ctgcaatagg cttcgttgaa
    28201 gtcttccgca tcctgccgcc acgccgtgag gacgacgata tccgagatgc ctgcgtatgc
    28261 ggcttcgata ttgccgtctt ttaccgcctg ccaataggct ttgtgcgccc ggtacaacct
    28321 ttcgactcga gcgttcggac tgcattccga atgttccagt tcatccggaa accggtcaaa
    28381 caatgcccac gccccctcat cgcccgatac ggcggcacgg gcaaggcagc cgatgccgct
    28441 gttgtcgccg aagcggtggc tgaacgacag atgggcggtg ttgtgcgcca acacgggcgg
    28501 atttgcgctg acgctgaaac cgtgttctgg aaggaagccg gccagccttt ggtgcgtttc
    28561 tccgtccaaa acggtttttt gcgacaaaac ggacagcacc gccccgattc cgacggacgg
    28621 gagctggttt tcatccccca gcagaatcac gcgcgcgccg gttttgaccg cttttaaaag
    28681 ttgcaacatc aatgcggtat ccaacatgga ggcttcatcg acaatcagca catcgaacgg
    28741 cagcggacgg atatggtcga acgccgcctg cattttgggc gggcgcagct tcagcaatcg
    28801 gtggacggtt tgcccttcca gtttgagcaa atggcggcgg acggcctccg gcgcgtcaaa
    28861 accgttgatt gcacggtgca gcgcgcgcgc catatgtgct gccgctttgc ccgtcggtgc
    28921 ggcaagcgcg atatggggaa gattttcgtc ttcaccgcaa atcagcgcca gcagtttggc
    28981 aaccgttgtc gttttgcccg ttcccggccc gccggtaatc accataaaag actgtaacag
    29041 tgccaaggcg gcggcatcgc gctgtccttc gctgcccgcg ccttgaaacc attttgcgag
    29101 gttttgccgc gcacctgtcg cgtcgggggc ggatgtgccg gctgccgcca agcgttttat
    29161 ctcggcagcc aaatcgtatt ccaactgcca catcctgccc aaaaacagcc ttctgccttc
    29221 caaaatcaaa ggcgcggcgg atgttccgac cacgggtgcg agtgccgaca gcgcatcagc
    29281 ctcgccaccg ctcaaacgga taaacgaatg accgttctgc aatgcctgaa acaggcgttc
    29341 ggtacagttt gcaagcactt cgtcgccaga acccgcatag tgttccaaaa aacggattgc
    29401 cgcccttgcc gccgcttggg caaattcatc tgtctgccgt tccatcgtat tccttgtcga
    29461 aatgccgtct gaaggcgtgg ggcttcagac ggcgcggtgt tgtttcggct gtttaggcgt
    29521 ttgcgccctg tttggggtgc aggctgccgt ctttcatgac catcacgcgc ccgaagcggc
    29581 cggcgagttc gtcgtcgtgc gttacgacca ccagccccgt tcccaattct gttttcagtt
    29641 ccagcatcat atccaaaaca ttccttgcgt ttgctcggtc gagattgccg gtcggttcgt
    29701 cggcaagcag gcatttgggt tgcgtaacca aggcgcgcgc aatggcggca cgctgccgct
    29761 caccgcctga aagttcgccc gcgcggtgcg tcgaacggtg tttcagtccg accttgtcga
    29821 gcatcgacat tgccgtttct gccgcctctt cacggctttt tttgccgatc agaagcggca
    29881 tcatcacgtt ttccagtgcc gaaaattcag gcagaagatg gtggaactgg tacacgaaac
    29941 cgagatggcg gttacgtaaa tcgcccaaac gccgctggtt taaggtacgc aaatcctcgc
    30001 ccatcagcag caccctgcct tcagacggca tatccagccc gcctagaata tgcagcagcg
    30061 tcgattttcc gctgcccgaa gaaccgatga tgccagtgct ttcccccgcg cggatttcca
    30121 aatccaagcc gtgcagcacc cgaacgtcca aaccgccgtc ccgatagcgt ttgcccacgc
    30181 cttcgcattt caaaatcaac tcactcataa cgcaaagcct ccgccggttg ggtttttgac
    30241 gcgcgccggc tcgggtagag cgtcgccacg aaagacagcc ccaaggaaat gcaggcaatc
    30301 agggcaacgt cgcccatatc gacatcgctg ggcaggtagt cgataaaata aacctgcgaa
    30361 ttgatgaggt ggacaccgag caggttttca aaaaacgcca cgaccctgcc gacgttccaa
    30421 cccaaaagca cgccgcagac cacacccgcc aacgtgccga aaaagcctga aaacgcgccc
    30481 tgcaccataa aaatcttcat cacgccgcca ggggaaagac ccaaagtccg taaaatcgca
    30541 atgtccgcct gcttttccgt aaccgccatc accaaggaag agacaaggtt gaacgccgcc
    30601 acggcgataa tcagcgtcag gatgatgaac atcatccgtt tttccaactc gaccgcctca
    30661 aaatagctgc ggttgctgaa cgtccaatcg cgtacccaaa ccgtatccct ttgcgcctcc
    30721 ggaatcagct ttgccgtcaa ggcgggggcg ttttgcgggt cggcgagttt cagccgcagc
    30781 cccgcaactt ccttatccaa gcggtacagt acgcgcgcgt cttggatatg cgtcatcgcc
    30841 aatgagttgt caacctcgta aacacccgtt ttaaccagac cgaccacggt aaactgtttc
    30901 aacctcggta cgactccggc gggcgtaaca ttgccctccg gcgtgatgac ggtaacttta
    30961 ttgccgactt ccgcccccaa agcctccgcc aagccgacac cgaggataat gtcaaactcg
    31021 cccggaatca ggtcttcaaa tttgcctgcc ggcattttgt cgccgtattc caccactttg
    31081 cgctcttcag acggcaaaat gccgcgtatc tgcacgcccc tgatttcgcc cgcattggcc
    31141 agcaatgcct gattggaaac atagggcgcg gcagccaaaa tacctttgcg gttttcggta
    31201 aaccgaagca ggttgcgcca atccgtatcc gtattgtcga tatagccgat ttcggcgtgc
    31261 ggcgcgacat tcaggagctg cccgcgtatt tctttctgaa agccgttcat aaccgacaag
    31321 acgacaatca gcgcggttac gcccaaggcg attccggcaa tcgaaaccat cgtgataaac
    31381 gacataaagc cgttgcgctt tttcgccctg agatacctca agcctatcca agcctctaga
    31441 gaaaacataa cgctacctta aaaatgtctg caaacgtgcc gcccccggac ggcggtttgg
    31501 gggcgacggc aaaagtttat tgtaccgtaa aacccaggca gcgtccgaac gcccgagtgc
    31561 gggggacggg gcggcagagc gggcggaaaa cttgcacaac gcgtcaaact gccctatcct
    31621 ttccttcaga aaaaccgttt cttgaggaaa acaatgaata tccgaactgc ttttgctttg
    31681 tgcgccatcg ccttatccgc cgctgcggct gcctacgcca aagaaatcaa aatcgatgcc
    31741 aacaacacgc cttattccga agccgacgcg caaaagctgg cggcaacggc agtcggtatg
    31801 ggtgttaagg aacctatcag cctgaacggc ggcagcggca gcattaccgt gtccggcagc
    31861 agcgcgacgc agtgcgtgtt caaagtcggc aacggaggcg cgttgcagat tcaagggcta
    31921 aactgcaagt aaaccgcccg gaaaaatgcc gtctgaaggc ttcagacggc attttgcatt
    31981 ggcggcgtta tgccccgcct tctttaatca ggcggcgttc gtacaccgcc tgcgccagcg
    32041 ttcccgcatc gacatattcc aattcgccgc ccaagggaat gccctgcgac agcctgctga
    32101 ctttgtaagg caggttttta aaaaactcgg acaggacata cgccgtcgca ttgccttctg
    32161 cggtaaaagc ggttgcaata atgatttctt cgacttcccc gccgcccagc cgttgcgcca
    32221 gcctgtccaa tgcgatggcg gatacgtcca ttcccaatgc cgtattgatt tgccccatca
    32281 ggacgaaata cagcccgtcg tggcagtttg ccgcttccat attcgacacg tcggcaggca
    32341 tatgcaccac catcagccgt cgcccgtcgc gtgtttcatc ggcacaaata tcgcacaatc
    32401 cgccttcgca aaacgtgttg cacctcgcgc aatgatcgac ctgcttcaat gccgtctgca
    32461 aggcatccac cagtttttca gcctctttgc gcttgtgttg gagcaaatga tacgctatcc
    32521 gctgtgccga tttcggcccg acgttgggta aaaccttcag cgcgtcgatc aatccttgga
    32581 aggcatcttg ttttttgtgg ctcatcatat tccgccgtat gggaaaacgg ccggaatatt
    32641 ccgaccgtta ttttgtcaac aaaagtgtca attactgacc gtcgccgttg tcgaccgatt
    32701 gcgctccttt ggtctgtttg attttgccgt tgaaataacg tatcaacaag tcgaaagtat
    32761 tggcagactg ctgttgcgcc aaagcctgtt ttgcaagcgg aagctgtgcg gcgatatcat
    32821 ccggcggggt tacagcctgt acttcgacaa tcacgggtgc cggcagaccg atcagcctga
    32881 cgtaggcggg tttgccgttt gccggttttg ctttcagcag ttccgcataa gcctcgggcg
    32941 gcatggactg ccttgcctgc tgtgcgccca aaacggacac ttccgaccat ttcacgtcaa
    33001 cagccttgcc gccgttcagt tgggtaagca cgtcttttgc cttgttttcg gcaagtttgg
    33061 cggcttcggt acggatataa gcctgacgta ccgcgtcttt ggcttcggca aacggcaggg
    33121 ttttctcttc gcggacttct ttggcgcgga cgacccacgc ggtttcgctg ttgatggtca
    33181 gcacttcgga attgtgtttt ttcttcaata cgtcgtcgct gaatacggca ttgatcaggt
    33241 tttcgggcat accggacatt tgcgcatcct gcctgctcag ccaagtttct tgggtttcga
    33301 ctttcaaacc gctgtttttg gcggcttcgg caagcgagga aggatggttg aacgcatcat
    33361 cgcccagctt ttcttttgcc ttattgaagt cggcaaccgc ctttttcatt ttcaattcgt
    33421 tttcgacggc ggctttttcc tgctcgaaag aaggtttggc ttcatttgcc ggcaaacgcg
    33481 ccacgcgctc ttcaaacgca tttttcactt ctgtttcgct gacggtctgt ttgtctgcaa
    33541 agtctttcag attcaaggcg acatattcca atttgaccgc tttgggaagc aggtagtctt
    33601 ttttgtttgc gttataaaac ttctgcaaat cggcttcaga cactttgact tgggcgatga
    33661 attcgtcggg gttgaaagtg tgcgaacgga tggtgcggtt gacctgcgtc agcctgatca
    33721 gctgttccgc ctgcgcgtcg ccgaccaata cgccgttttg gacgaggttt accaaattct
    33781 gcaaggcaaa ctgatcgcgg atttcttcga caaactggtc ttcagacata tgacgttggg
    33841 aaaggtagcg gtttaaaagc gcgtggtcga atttgccgtt tgcgtcgtgg aaattgggat
    33901 cgtccacgat aatctgcttg atttgttcgg aagaaaccga aatgcccatc agcttcgcgc
    33961 cctgtttcag gtaggcgcgt tgtagcaggg attggaacac cgcgtcgcgc gaagggccgc
    34021 cgccgtccgc ctgttcgttc tgtatggcgt tgttgatgga gtggtcgctg attttttcgt
    34081 cgcccacttg gacgatgtag tcggcacccg gatgggatac cgtgctgacc ccgaagccga
    34141 cgaaggttaa tgcaatcagg cccaaaagga cttgggcggg cgttctgtat ttttcgatgg
    34201 aatggaacat attttaaatc gggatataga atgggaaatt caagtcgggt attgtaacgg
    34261 tttttatccc tgtctgcacg gggcttgccg gttgaagatg ccgtcgtagg tttcttcgct
    34321 gaagctggcg taaaccttgc cgccgcactc caatacggga cgcttgatca ggctcggcat
    34381 ttcggacatc agtttgacgg cctccgccgt cgaggacagc actttttgct gtgtttcggc
    34441 atcgagtttg cgccagcttg tcccgcgttt gttgagcagg gttgccaaag gcacttgttc
    34501 cagccacgag cagatttccg cttcagacgg cctctgtttt ttaaaatccc gaaattcaaa
    34561 ctccaagccg tatccggcaa gccggttttt ggcttttttg accgtatcgc aatttgggat
    34621 gccgtgaagg attatcattt ggaaaccttt tgtctgaaat aataaaacgg atattttact
    34681 ataagtgtct gaaaatttgc ccgtctgttt cagacggcgg ggcggttatg ttacaatccg
    34741 aaaattcgaa aaatttaatc tcttgttcaa taaaggcttt accaatcatg atttctacca
    34801 acggcatcac catgcagttc ggcgcgaagc cgctgtttga aaacgtatcc gttaaattcg
    34861 gcgaaggcaa ccgctacggt ttgatcggcg cgaacggctc aggcaaatcc accttcatga
    34921 aaatcctcgg cggcgatttg gaacagacag ccggcgaagt ggcgattgaa aacggcgtgc
    34981 gtttgggtaa attgcgccaa gaccagtttg cctacgaaga tatgcgcgtg ctggacgtgg
    35041 taatgatggg tcacaccgaa atgtgggcgg cgatgaccga acgtgatgcg atttacgcca
    35101 atcccgaagc caccgaagac gactacatga aagccgcaga actggaagcc aagttcgccg
    35161 aatacgacgg ctacaccgcc gaagcgcgtg ccgccgagtt gttgagcggc gtgggcattt
    35221 ccgaagattt gcacaatgca accatggcag aagttgcccc gggcttcaaa ctgcgcgtat
    35281 tgctggcgca agccctgttc tccaagccgg atgtattgct cttggacgaa ccgaccaata
    35341 acttggacat taataccatc cgttggttgg aaggcgtgtt gaaccagtac gactccacga
    35401 tgattatcat ctcgcacgac cgccacttct tgaacgaagt ctgcacgcat atggcagatt
    35461 tggattacaa caccatcacc atctatccgg gcaactacga cgactatatg ctcgcctccg
    35521 cccaatcgcg cgaacgcgcc ctgaaagaca atgccaaggc gaaggaaaaa ttgcaggaat
    35581 tgcaagaatt tgtggcgcgg ttcagtgcca acaaatccaa agcccgtcag gcaaccagcc
    35641 gtctgaaaca ggcagacaaa atcaaatcgg agatggtcga ggtcaaaccc tctacccgtc
    35701 aaaacccgta tatccgtttt gaagccgatg aaaaagccaa gctgcaccgt caggctgtgg
    35761 aagttgaaaa actggcgaaa cgctttgaaa cccagttgtt taaaaacctg aactttatcc
    35821 ttgaagcggg acaacgcctc gccatcatcg gcccgaacgg cgcgggcaaa tccaccctgc
    35881 tgaaactctt ggccggcgcg tacaaccccg aatattcaga cggtctgctg ccggacgaag
    35941 gcagcatcaa atgggcggaa aaagccagcg tcggctacta tccgcaagat cacgaaaacg
    36001 acttcgatgt cgatatggat ttgagcgaat ggatgcgcca atgggggcag gacggcgacg
    36061 acgaacaagt catccgcggc actttggggc gtttgctctt cggcagtaac gatgtcgtga
    36121 aaaaagtgaa ggttctctcc ggtggtgaaa aaggccgtat gctttacggc aaactgttgc
    36181 tgttgaaacc caatgtctta gtcatggatg aaccgaccaa ccacatggac atggaaagca
    36241 tcgaatcctt gaacatggcg ctggaaaaat acaacggcac gctgattttc gtctcccacg
    36301 accgtcagtt cgtttcttcc ctggctaccc aaatcattga attggacggc aaaggcggat
    36361 atgaacacta cttgggcgat tacgaaagct atctcgagaa aaaaggcgta gcataaccgc
    36421 cggttggaac aatgccgtct gaagcggctt cagacggcat tgttgataac tttaaaatag
    36481 gaagcatatg cagacttatc tcgtcggcgg tgccgtccgc gattatcttt tgggcttgcc
    36541 cgtcaaagac cgcgattggg tggtcgtcgg cgcagacgca caaaccatgc tggcgcaagg
    36601 cttccagcca gtcggcaaag actttcccgt gtttctccat cccgaaacac acgaagaata
    36661 cgccctcgcc cgcaccgagc gcaaaaccgc caaaggttac gtcggtttca gtttccacgc
    36721 cgacaaagac gttacgctgg agcaggattt gatgcgccgc gacctgacca tcaacgcgat
    36781 ggcgcaagat gcggacggca agattatcga ccctttcggc ggacaacggg atttggcggc
    36841 aggcattttg cgccacgttt ccccagcctt tgccgaagac cccgtccgca tcctgcgtac
    36901 tgcccgcttt gccgcgcgtt acaagtttga aatcgccgaa gaaaccataa agctgatgcg
    36961 gcagatggtg gaaaacggcg aagcggacgc attggttgcc gaacgcgtct ggcaggagtt
    37021 tgcgaaaggt ttgatggaaa aaaatccgcg caaaatgatt gaagtgttgc gcgaatgcgg
    37081 cgcgctcaaa gtcttgctgc ccgaagtcaa tgccctcttc ggcgtgccgc aacgcgccga
    37141 ctaccatccc gaaatcgaca gcggcatcca taccctgatg acgctgcaac gcgccgccga
    37201 tatgggtttg agcctgcccg aacgctatgc cgccctgctg cacgacttgg gcaaagccaa
    37261 aacaccgtcc gacatcctgc cgcgccacca cggacacgac ctcgccggtg tcgaacccgt
    37321 gcgcaaagtc aatcaaaggc tgcgtgcgcc gaaacattgt gccgagcttg ccgaattggt
    37381 ttgccgttgg cacattattt tccaccaagt cggacagctt aaaagccaaa ccattctgaa
    37441 cgtattgaaa aaaaccgacg ctttcagacg acccgaacgc tttcagacgg cattgaacgt
    37501 ctgcattgcc gacacgcaag gccgtctgaa ccgcgaacac acgccctacc cgcaacgcgc
    37561 gcactggctc gccttactcg aagccgccaa tcaggcggat tcgggcaaaa tcgccgccga
    37621 atgccgctcg cagggaaaag cgcaccttat cgccgaacaa atcgaccggg cgcggctggc
    37681 acaaatcgcc ccattgcaaa aagcgtttcg agcggcgcaa gacaaaacag aaaaacatta
    37741 aaacgtccaa tgcagcca
//
LOCUS       DAWKOG010000005        30725 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.005, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000005 DAWKOG010000000
VERSION     DAWKOG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30725
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.005"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            142..1110
                     /locus_tag="WJK66_000184"
     CDS             142..1110
                     /locus_tag="WJK66_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ7577957.1"
                     /translation="MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMLLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     ENEMLQMLGVWQQGDFSGVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFIGLVVPNIISRLMGDKLRQSLPAVALLG
                     ASLVLLCDIIGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAHAV"
     gene            1100..2074
                     /locus_tag="WJK66_000185"
     CDS             1100..2074
                     /locus_tag="WJK66_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEZ7577958.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCVLFMTLNVKGDWDFVL
                     QLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYASLPLTGKFGFELAVMMGGSLLLFYTLIRQGGRDLPRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVHSELLGIGALILLVSAAVVWRERYRLDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            2119..2745
                     /locus_tag="WJK66_000186"
     CDS             2119..2745
                     /locus_tag="WJK66_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7577959.1"
                     /translation="MTQERLPEFFDRAPTLTVQDPLAAFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAAMQGARDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRNLLSFGAGEINGTLTLRRKDNGKTVAVSLNAALQPFAPE
                     MRELMPKTVGGSASAEELERFGQLWQARVKAFLTESADDPQFVIVREV"
     gene            2829..3587
                     /locus_tag="WJK66_000187"
     CDS             2829..3587
                     /locus_tag="WJK66_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ7577960.1"
                     /translation="MITIRNVSYRIGTHPILDNISLDIPEGGITALIGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLAGTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDFADRYLTELSGGQRQRAMIAMVFCQRTDYVLLD
                     EPLNNLDMYHARSLMQILRRLTDEHKRTTVVVLHDINQAAAYADHVVAMKNGQVAMQG
                     KPDDIFTAANIKTLFDMDVSVLDHQSKKLIVHHI"
     gene            complement(3646..3984)
                     /locus_tag="WJK66_000188"
     CDS             complement(3646..3984)
                     /locus_tag="WJK66_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEZ7577961.1"
                     /translation="MKKIEAVIKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERADAAV"
     gene            4079..4291
                     /locus_tag="WJK66_000189"
     CDS             4079..4291
                     /locus_tag="WJK66_000189"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7577962.1"
                     /translation="MPETVIPAQAGIPTSAFQQYLKVSVNPNFHIPDRAETTAFDPKT
                     YGSSCPLLCFLQLSAYPALGKNQKNR"
     gene            4449..8414
                     /gene="purL"
                     /locus_tag="WJK66_000190"
     CDS             4449..8414
                     /gene="purL"
                     /locus_tag="WJK66_000190"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEZ7577963.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFVG
                     SEKALDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDRQKQQWAALLHDRMTESVLTDIDAAAQLFHHIQSET
                     FSTVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     DAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPGLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGDKNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFELREVPLEEHGLTPLQIWCNESQERYVLSILEKDLETFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFANNPVDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHAGDIDITEAAYRVLRLPTVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPTVALFDAPASGRMCVGEAITNIAAANIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVQDVRKTVTPELKNVEDSVLLFVDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDTGRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFAVLVEMAFAGRCGLDIDLNLLLA
                     QTFITNHTALSQSLRTEEVKALAEWQETIARTLFNEELGAVIQVRKQDVADIINLFYQ
                     QQLHHNVFEIGTLTDENTLIIRDGQTHLISDNLIKLQQTWQETSHQIQRLRDNPACAD
                     SEFALIGDNERSALFANLKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFT
                     RAGFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQ
                     FAAFFADPDTLTLGVCNGCQMVSNLAEIIPGAAEAWPKFKRNLSEQFEARLSMVHVPK
                     SASLILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQTYPLN
                     PNGSPQGIAGITNADGRVTIMMPHPERVYRAAQMSWKPEDWTELSGWYRLFAGARKAL
                     G"
     gene            8509..9261
                     /gene="gloB"
                     /locus_tag="WJK66_000191"
     CDS             8509..9261
                     /gene="gloB"
                     /locus_tag="WJK66_000191"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEZ7577964.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAERTPTLPVTLAHERRVNPFLRTEIP
                     AVRQRAEALVGKTLNSGLEVFAALRELKNAYC"
     gene            9495..10949
                     /gene="mgtE"
                     /locus_tag="WJK66_000192"
     CDS             9495..10949
                     /gene="mgtE"
                     /locus_tag="WJK66_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEZ7577965.1"
                     /translation="MSIEPTPPNLENDGIENDVERVSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLGELHPADVAAVLESLPPRERNVVWLLVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRVTVDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTDMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMVAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(11035..11943)
                     /gene="hslO"
                     /locus_tag="WJK66_000193"
     CDS             complement(11035..11943)
                     /gene="hslO"
                     /locus_tag="WJK66_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEZ7577966.1"
                     /translation="MNQTAINRADVRTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGRGRLKMLVAEAASDRTVRATARWDET
                     AEIADDESLGDLLGEGGVFVLTLQPKDGEPWQGVVPLEGGGIAQMLANYMKRSEQIDT
                     HIVLSASDEAAGGLLVQRLPEAVLDEEAWEHVSTLARTLTADELAELDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVVEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            12188..12943
                     /locus_tag="WJK66_000194"
     CDS             12188..12943
                     /locus_tag="WJK66_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ7577967.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            12962..13180
                     /locus_tag="WJK66_000195"
     CDS             12962..13180
                     /locus_tag="WJK66_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7577968.1"
                     /translation="MSMPEMPKWYDDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            13325..13672
                     /locus_tag="WJK66_000196"
     CDS             13325..13672
                     /locus_tag="WJK66_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEZ7577969.1"
                     /translation="MNIIRALLIILGCLATGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMSNLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRGIIR"
     gene            13669..14361
                     /locus_tag="WJK66_000197"
     CDS             13669..14361
                     /locus_tag="WJK66_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEZ7577970.1"
                     /translation="MNEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILGIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            14428..15648
                     /gene="argJ"
                     /locus_tag="WJK66_000198"
     CDS             14428..15648
                     /gene="argJ"
                     /locus_tag="WJK66_000198"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEZ7577971.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALVINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            15811..17220
                     /locus_tag="WJK66_000199"
     CDS             15811..17220
                     /locus_tag="WJK66_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ7577972.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLASGFI
                     QVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLAAAKWLAT
                     VFSYWAGIPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRQRVLQETAAQTG
                     NAPARPQTANSKTGMPSEN"
     gene            17410..18801
                     /gene="hrpA"
                     /locus_tag="WJK66_000200"
     CDS             17410..18801
                     /gene="hrpA"
                     /locus_tag="WJK66_000200"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEZ7577973.1"
                     /translation="MFPDFSQTLSKDRHFLQSAFKNPNKYGGLSKVEEKYRKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLDEIKKAIAENQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSRHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARHGEGDI
                     LVFLPGEREIRETAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHIEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEEDFNSRPEFTDPEIVRSNLAAVILRMAALKLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAV"
     gene            18945..20588
                     /locus_tag="WJK66_000201"
     CDS             18945..20588
                     /locus_tag="WJK66_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ7577974.1"
                     /translation="MDEIQIPKKVELQTKLENEKIVLSKGSTTIIVGANGTGKTRLAV
                     YIEEQLKEKAHRISAHRALKLNPNVNKIPEKSAKTYLSYGQNWDGIDVSNRKNYRWDN
                     NSYTHLLNDFDWLLQYLFAQQNNIAVANNQKLNRNEKVTNSKTKLDILQEAWETLLPH
                     RKLHITADDIQVSAVDNEELYSASNMSDGERALFYILGQVLSVDDGSVLIFDEPELHI
                     HKSIISNLWDKIEELRPDCSFLIITHDIEFAATRVAKKYVIRNYYPTPAWDISEVPES
                     NFDEETITMILGSRKPILFVEGNNNSLDIATYRYCYPDWTIIPKGACKDVIQSVSSLK
                     KLSNEMPLLNLKCSGIVDLDSRDEREIEQLNNLGIYILPVSEIENLFSLTDVAKEILK
                     LNQYSDEELLNKLNGFKSELIKYIDNELKDDKLDEFVVKQVRRKIDNYLKNIDLSSKI
                     TSTDMKKSLLNEISTLTEQKIETWISEIKNEIQRCIEQQDLDKLLTIYDNKGLLAKSA
                     CVLKGMRNKHEFESWIMRTLKGRNKDFIDAIRQKLPILD"
     gene            20632..20814
                     /locus_tag="WJK66_000202"
     CDS             20632..20814
                     /locus_tag="WJK66_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7577975.1"
                     /translation="MKNHQTTLSFPTSSSLKPCADKQGLSEDRSQYRLTKLGEQMAHL
                     PIDPKIARILLVLFRF"
     gene            21026..22603
                     /gene="lpt3"
                     /locus_tag="WJK66_000203"
     CDS             21026..22603
                     /gene="lpt3"
                     /locus_tag="WJK66_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HEZ7577976.1"
                     /translation="MMKKSFLTLVLYSSLLTASEIAYRFVFGIETLPAAKIAETFALT
                     FVIAALYLFARYKVTRLLIAVFFAFSIIANNVHYAVYQSWMTGINYWLMLKEITEVGS
                     AGASMLDKLWLPALWGVLEVMLFCSLAKFHRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSRIPAFKQPAPSKIGQGSVQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTRLSQADFKPIVKQSYSAGFMTAVSLPSFFNAIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENEMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIVDK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYLVPL
                     VLYSPDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLNIRDGKAEYVYPQ"
     gene            22705..25968
                     /locus_tag="WJK66_000204"
     CDS             22705..25968
                     /locus_tag="WJK66_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase"
                     /protein_id="HEZ7577977.1"
                     /translation="MDARSNPANVSDGLQNSSGHIGTNTRYRLTKLGEQMARLPIDPK
                     IARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHERFTDKQSDFLAYL
                     NIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQTAIEMGLTTKEAA
                     FRRLSEIKQLTSSENQGDQDLSAKRKQKQLDKKQHRAQIRAAKEAGYEQIHRALLTGL
                     IANVGMKSPDGNDYTGARGSRFHLFPASALFKAKPKWVMAAELVETTKLYARDVAVIQ
                     PEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRPVPYGKVAPEEAR
                     EIFIRSALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDDEALFAFYNERLP
                     EMAWKDAQGSVWGSEDSVRIIESDKAERSSENERNEFRKNKRNGSRQNENHGNTVGWV
                     ENPTSAATAKTVGFDNPTYATQQPTPSPAREGRGEGKTVAAQTNFSATAANPLPQERE
                     QSAAASAVSNDLHPANPQQTAPSPVGEGRGEGKTVASQTNFSATTANPLPNPLPQEGE
                     QSAAASAVSNDPQPQKQPAPQKDRLKPLPLADIRTFQAWLKTAERENPRLLFLSRDDL
                     MQHAAAHITEEQFPKFWQTADGKFKLSYRFEPHHPLDGVTLTLPLTVLNRLHAPSLEW
                     LVPGMLREKIQLLIKALPKQIRRICVPVPDFITKFLESNPDRQAVIIPQLAHFIAKSA
                     GDMRIFEQIDQDAWAAQELPEHCYLNLLIIDDGGQELAGGRKLHELQQQLGQAAAVTF
                     RDNTQEFERDNVTTWDIGTLPESIKFARGKQQLTGYLGLQKEKDGRIALRLCDTTEAA
                     EQAHRQGVIELMKLQLKEQVKDLNKGIQGFTQAAMLLKHINADTLRDDLTQAVCDRAF
                     IGEDELPRNEKAFKEQIKRARSRLPAVKEALSRYLQETAAAYAELNGKLGKHPLTHLL
                     RLRLQTLLAAGFATRTPWAQWPRLPIYLKAMTLRLEKYSSNPARDAAREADIQELEQM
                     WQEKNDGLVKQGLPVSDDLTAFKWMIEELRVSLFAQELKTPYPVSVKRLLKEWEDLN"
     gene            complement(25991..26413)
                     /locus_tag="WJK66_000205"
     CDS             complement(25991..26413)
                     /locus_tag="WJK66_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HEZ7577978.1"
                     /translation="MTDIFTTSKRSFVMSKIHSKETKPEVLVRKFLFFQGFRYRKNDK
                     RYVGKPDIVLSKYKTVVFIHGCFWYGHSCNKGHIPKSNTDFWLEKITKNCERDIKNET
                     ELEKIGFKVIVVWECELKNKAICRERLNRLVREIKDAV"
     gene            complement(26417..28063)
                     /locus_tag="WJK66_000206"
     CDS             complement(26417..28063)
                     /locus_tag="WJK66_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HEZ7577979.1"
                     /translation="MSEEKLKMSFEPTVIEHLGVKMYSHTVPAIAELIANAYDACATE
                     VEVRLFDKPEHKIVIKDNGIGMSFDEINDFYLRIGRNRREEKQASPCGRIPTGKKGLG
                     KLALFGLGNKIEISTIQGNERVTFTLDYAEIRRSKGIYQPEFRKESVESNIESGTTIT
                     LTELTKKQGYPLDNYVEHLSRLFDFPAQDFKIKVSLNGSEPKIIDGNLKYDLVTPQFE
                     WEYQDLATNISSLSSKFEQYEYSGLIQGKFITTEKPLKNNMKGITLFANGRMVNMPEF
                     FTDSESSHFYSYLTGWLNVDFIDNDDEDLIATDRTSLDWKHSKTSKLRVFLGEIVRAI
                     GQDWRKRRKKEKDDEVKGESGVDIEDWKNKLPEKEREPVEVILNRLEDSELTNKEQAE
                     VISALHSIIPEYPYYHWRHLHQDLHTACNDFYNEKKDYLSAAIEAVKVFEDKVQKQTG
                     LHSIDGRELIEQAFGSKNSILLLTNNKTKAEQNLEDGLEQLACGTWTGFRNPVQHELR
                     ANLSPSIFNDKDALDLISLVSYLLRKVEQTKKRSKVVSSK"
     gene            complement(28077..29132)
                     /locus_tag="WJK66_000207"
     CDS             complement(28077..29132)
                     /locus_tag="WJK66_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ7577980.1"
                     /translation="MKNSKLKAVDFFCGGGGMSYGMQSAGIQVLAGIDYEPSCKETYE
                     ANINAKFIQADVFELQPETLEKELGLKKNDDDLILIGCSPCQYWSVIQTDKRKSEKSK
                     SLLLEFQRFVEYFNPGYVVVENVPGILSRMKESRLDNFIKLLEEKGFTVHFGIHNTAD
                     YGIPQSRKRFTLIANRITKEKLEPVKYSGKRLTVRDVLGMENGFPKIIAGHQDETDFM
                     HSCAGLSDINLKRLALIPKNGGNRLAFAHIPELQLECFIGKDNSFKDTFGRLWWDKPA
                     PTITTKFFSISNGRFAHPEEDRALSLREGATLQSFPRNYVFKAGSRDKIARLIGNAVP
                     PMYAEKIGRAIVDNIEC"
     gene            29422..29634
                     /locus_tag="WJK66_000208"
     CDS             29422..29634
                     /locus_tag="WJK66_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7577981.1"
                     /translation="MPRIHPPHLFPPHIAERGLLYFQQGKVRDVRKTSAGRYRAEVCG
                     LENYWVWLKLDSDLYIKDESCDCPYV"
     gene            29944..30099
                     /locus_tag="WJK66_000209"
     CDS             29944..30099
                     /locus_tag="WJK66_000209"
                     /inference="COORDINATES: protein motif:HMM:NF017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEZ7577982.1"
                     /translation="MSERKTKPSPKKGVVFPFIKKLLSINEINPNDPTIGFILLSRNN
                     PDTDYES"
     gene            complement(30189..30638)
                     /locus_tag="WJK66_000210"
     CDS             complement(30189..30638)
                     /locus_tag="WJK66_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ7577983.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINTVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT         7639 a         8674 c         7288 g         7124 t
ORIGIN      
        1 gcgggaatgg cggcaagcgt ttttattgga acttgcccta tcctctgccc gatacggagg
       61 cttcaaacaa ggctttccgc tccgacggtt cggactgcct tgtttgaatc ttctacgcct
      121 taacgctttt cccttctgtt tatgactgcc aaaccttttt ccctcaacct gaccaacctc
      181 ctgctgctgg cggtgttgtt tgccgtcagc ctgtcggtcg gcgttgccga tttccgctgg
      241 tcggatgtgt tttcgctgtc ggacagccag caggttatgt tcatcagccg cctgccgcgc
      301 acgtttgcga ttgtgctgac gggcgcgtcg atggcggtgg cggggatgat tatgcagatt
      361 ctgatgcgta accgttttgt cgaaccttct atggcgggcg cgggtcagag tgcggctttg
      421 ggtttgcttc tgatgtccct gctgctgcct gccgcgccgc tgccggtcaa aatgtcggtt
      481 gccgccgttg ccgcgttaat cgggatgctg gtgtttatgc tgctgatccg ccgtctgccg
      541 ccgaccgcgc aactgatggt gccgctggtc gggattattt tcggcggcgt ggttgaggcg
      601 gtggcgacct ttatcgcgta tgaaaacgaa atgctgcaaa tgctcggcgt gtggcagcag
      661 ggcgattttt caggcgtact gctggggcgg tacgagctgc tttggattac gggcggtttg
      721 gcggtgtttg cctatctgat tgccgaccgg ctgacgattt tggggctggg cgagacggtc
      781 agcgtgaatt tgggtttgaa ccggacggcg gtgttgtggt cggggctgat tattgtggct
      841 ttgattacgt cgctggttat cgttacggtc ggcaatatcc cgtttatcgg gctggtcgtg
      901 ccgaacatca tcagccgcct gatgggcgac aagctgcgac aaagcctgcc tgcggtggcc
      961 ttgctgggcg catctttggt gttgctgtgc gatattatcg gacgcgtgat tgtgtttccg
     1021 tttgaaattc cggtctctac ggtttttggt gtgttgggta cggctttgtt tttgtggctt
     1081 ttgttaagga aacctgctca tgccgtctga aaaaaatatc ggttttatgg caggaagcag
     1141 ccgtccgttg tgggtcgcct ttgcgctgtt gctggtttcc tgcgtcctgt ttatgacgct
     1201 caacgtcaaa ggcgattggg attttgtttt gcaactgcgg ctgaccaaac ttgccgcgct
     1261 gctgatggtc gcctatgcgg tcggcgtttc gacccagctt tttcaaacgc tgaccaacaa
     1321 tccgattctc acgccctcga ttttgggttt cgattcgctg tatgtgtttt tgcagacctt
     1381 gctggtgttt acgttcggcg gcgtgggcta tgcttccctg ccgttgacgg gcaaattcgg
     1441 ctttgaactt gccgttatga tgggcggctc gctgctgctg ttttacacgc tcatccgtca
     1501 gggcggacgc gatttgccgc gtatgatttt aatcggcgtg attttcggga ttttgttccg
     1561 cagcctgtcg tcgctgcttt cgcgcatgat cgaccccgaa gaatttacgg cggcgcaggc
     1621 gaatatgttt gccggattca ataccgtcca cagcgagctt ttgggcatag gcgcgctgat
     1681 tctgctcgtc agcgcggcgg tcgtttggcg cgaacgctac cgcttggacg tacacctttt
     1741 ggggcgcgac caagccgtca atttgggcat cagctacacg cgcaacacct tgtggatact
     1801 gctttggatt gccgcattgg tggcgacggc gaccgccgtg gtcggcccgg taagcttttt
     1861 cgggcttctc gccgcctcgc ttgccaacca cttttccccg tccgtgcgcc attccgtccg
     1921 cctgccgatg acggtttgtg tcggcggcat cctcctggtc ggcggacaga ccgtattcga
     1981 acacttcttg ggcatgaagg cggtattaag cgtggtggtc gaatttgcgg gcggactcgt
     2041 tttcctctat ctcgttttaa aacacaaaaa atgacggatg ccatctgaac ggcagcccgc
     2101 cccgaaagga caaaccatat gacgcaagaa cgtttacccg aatttttcga ccgcgccccg
     2161 acgctgaccg tacaagaccc gcttgccgca ttcctcggcg cggccgaaaa cggcatcctc
     2221 acttaccgct acgccgatgc cgtgcgcctg tgcggacatt cctgcccgac cgtcgcgggc
     2281 gcgtacctga tggttatcaa aggtctgaaa gccctttacg gagaagagct gcccgaacgc
     2341 ggcggcatcg aagccgccat gcagggagcg cgcgacgaag gcacggtcgg cgtaaccgcg
     2401 tccgtcgtcc aacttctcac cggcgcagcc cccgaaaccg gcttcggagg catcggaatg
     2461 cagggacgct tcgcccgccg caacctctta tcctttggtg caggcgaaat caacggcaca
     2521 ctgaccctgc gccgcaaaga caacggcaaa accgtcgccg tcagcctcaa cgccgccctg
     2581 caacccttcg cacccgaaat gcgcgaactt atgcccaaaa ccgtcggcgg cagcgcaagc
     2641 gcagaagaac tcgaacgctt cggacaactc tggcaggcac gcgttaaagc atttttgacc
     2701 gaatcggcgg acgacccgca gttcgtcatc gtccgcgaag tgtgagcgtt cagacggcat
     2761 tccgaatttc aaatgccgtc cgaacccccg ccaaacaaca acaaacctac gcccgacaag
     2821 catccgccat gattaccatc cgcaacgtca gctaccgcat cggcacgcac cccatcctcg
     2881 acaacatcag cctcgacatc cccgaaggcg gcattaccgc gctaatcggc ccgaacggcg
     2941 caggcaaatc caccctgttt tcctttatgg cgcggctgcg cccgcttgaa agcggcagca
     3001 tcgcctaccg cggcaaaaac cttgccggca cccccaccgc cgaactcgcc cgcacgctct
     3061 ccatcctcac ccaagaaaac agcatcatga gccgcatcac cgtgcgcgac ctgctgatgt
     3121 tcggccgtta cccctaccat caaggcagac cgactgccga atgccgccgt atcgttaacg
     3181 gtgcaatcga agaattccac ctgcaagact tcgccgaccg ctacctgacc gagctttccg
     3241 gcggacaacg ccaacgcgcc atgattgcca tggtgttctg ccagcgcacc gactacgttt
     3301 tgctggacga accgctgaac aacctcgaca tgtatcatgc ccgctccctc atgcaaatcc
     3361 tgcgccggct gaccgacgaa cacaagcgca ccaccgtcgt cgtattacac gacatcaacc
     3421 aggccgccgc ctacgccgac cacgtcgtcg ccatgaaaaa cggccaagtc gccatgcagg
     3481 gcaagcccga cgacattttc accgccgcaa acatcaaaac cctgttcgat atggatgtgt
     3541 ccgtcctcga ccaccaaagc aaaaaactca tcgtccacca tatctgaagc tcaaaaagaa
     3601 aatgccgtct gaaaaccttt cagacggcat ttccattcca aacgctcaaa ccgccgcgtc
     3661 cgcacgttcg cccgtgcgga tacggattgc ctcctcgacc ggcagcacaa aaatcttgcc
     3721 gtcgccgatt tttcccgaac gcgccacctc gacaatcacg tcaatcgcgc gttccacagc
     3781 atcatccgcc aacacaagct cgattttgac cttgggcagg aaatcgacgg cgtattccgc
     3841 accgcgatag atttccgtat gccccttctg cctgccgaac cctttgacct cgctgacggt
     3901 catgcccgta atgccgattt ccgtcaacgc ctcgcgcacg tcgtcgagtt tgaacggttt
     3961 gataacagct tcgatttttt tcatcacatt ctccattaat taacgtaaac aaacgacaaa
     4021 tatagcagaa accgccggca aacgataaac ggtactgttc gcccggcgat gcggcgattt
     4081 gccggaaacc gtcattcccg cgcaggcggg aatcccgacc tctgcatttc agcaatattt
     4141 aaaggtttct gtaaatccaa acttccatat tcccgaccgt gcggaaacga cggcatttga
     4201 tccaaaaaca tacggaagca gttgcccgct tctctgcttc ctgcaattat ccgcataccc
     4261 ggctttgggt aaaaatcaga aaaacagata aaacccttat tccttaatat cttcagacgg
     4321 cacctgtatt ttccccaacc gtatgccgtc tgaagattgt caacatttta agccaaaata
     4381 tccaagcaat acaaacctgt ccggcgtata atccctcttt ttcccaaccg catttgagag
     4441 ccgaatccat gtctgtcgtt ttgcccttgc gcggcgttac cgccctttcc gatttccgtg
     4501 tcgaaaaact cttgcaaaaa gccgccgcac tcggtctgcc cgaagtcaaa ttaagcagcg
     4561 aattttggta tttcgtcggc agcgagaaag cacttgatgc cgcgactgtc gaaaaactgc
     4621 aagccttgtt ggcggcgcaa agcgttgaac aaacgccaaa agcgcgcgag ggcttgcatt
     4681 tgtttttggt cacgccccgt ttgggtacga tttcgccgtg ggcttccaag gcgaccaata
     4741 tcgcggaaaa ctgcggtttg gcaggcattg agcgtatcga acgcggcatg gcggtgtggc
     4801 tggaaggtgc gcttaccgac aggcaaaaac aacaatgggc agccctgttg cacgaccgca
     4861 tgaccgaaag cgtgttgacc gacatcgatg cggcggcgca actgttccac cacatccaat
     4921 cggaaacttt ctccaccgtt gatgttttgg gcggcggtaa agaagctttg gtcaaagcca
     4981 ataccgaaat gggcttggca ctctccgccg acgaaatcga ttatctggtt gaaaactatc
     5041 aggctttgca gcgcaatccg tccgatgttg aattgatgat gttcgcgcag gcaaacagcg
     5101 aacactgccg ccacaaaatc ttcaacgccg atttcatcct caacggcgaa aaacaaccga
     5161 aatccctctt cggcatgata cgcgacacgc acaacgcgca tcccgaaggt acggtcgtcg
     5221 cttataaaga caattcgtcc gtaatcgaag gcgcgaaaat cgagcgtttc tatccggatg
     5281 cggcggaaaa ccaaggctac cgtttccacg aggaagacac gcatatcatc atgaaagtgg
     5341 aaacgcacaa ccacccgacc gccatcgcgc cgtttgcggg cgcggcgacg ggcgcgggcg
     5401 gcgaaatccg cgacgaaggt gcgacgggca aaggttcgcg tccgaaagcg ggcttgaccg
     5461 gctttaccgt gtccaacctc aacatccccg gcctcaaaca gccgtgggaa caagattacg
     5521 gcaagccgga acatatttcc tcgccgctgg acattatgat tgaaggcccc atcggcggcg
     5581 cggcgttcaa caacgaattc ggccgcccca acctcttggg ctacttccgc acctttgaag
     5641 aaaaatttga cggtcaggtt cgcggctacc acaaaccgat tatgattgcc ggcggtctgg
     5701 gcagcattca ggcgcagcag acgcataaag acgaaatccc cgaaggcgcg ttgctgatcc
     5761 aactgggcgg cccgggtatg cttatcggct tgggcggcgg cgcggcttct tcgatggata
     5821 ccggtacaaa cgacgcttct ttagacttca actccgtaca acgcggcaac cccgaaatcg
     5881 aacgccgtgc gcaggaagtg atcgaccgat gctggcagct tggcgataaa aacccgatta
     5941 tctccatcca cgatgtcggt gcaggcggct tgtccaacgc cttccccgaa ctggtcaacg
     6001 atgccggacg cggcgcggta ttcgagctgc gcgaagtgcc gttggaagaa cacggtttaa
     6061 cgcctttgca aatctggtgc aacgaatcgc aagagcgtta tgtattgtcg attttggaaa
     6121 aagatttgga gaccttccgc gccatctgcg aacgcgaacg ctgcccgttt gccgtagtcg
     6181 gcacggcgac cgacgacggc catttgaaag ttcgcgacga tttgttcgcc aacaaccccg
     6241 tcgatttgcc gctgaacgtc ttgctcggca aaccgcccaa aaccacgcgc accgacaaaa
     6301 cggttacgcc gtccaaaaaa ccgtttcacg ccggcgatat cgacatcacc gaagccgcct
     6361 accgcgtttt gcgcctgcct accgtagcag ctaaaaactt cctgattacc atcggcgacc
     6421 gcagcgtcgg cggcatgacc caccgcgacc aaatggtcgg caaataccaa acccccgtag
     6481 ccgactgcgc cgttaccatg atgggcttca atacctatcg cggcgaagca atgtctatgg
     6541 gcgaaaaacc gaccgttgcc ctgtttgacg cgcccgcctc gggcagaatg tgcgtcggcg
     6601 aagccatcac caacatcgcg gcagccaata tcggcgacat cggcaacatc aaactctccg
     6661 ccaactggat ggcggcgtgc ggcaacgaag gcgaagacga aaaactctac cgcaccgtcg
     6721 aagcggtttc caaagcctgt caggcattgg atttgagcat tcccgtgggc aaagacagcc
     6781 tgtcgatgaa aaccgtgtgg caggacggcg aagagaaaaa atccgtcgtt tcgccgttga
     6841 gcctgattat ctccgcgttc gcgccggttc aagacgtacg caaaaccgtt acgcccgaat
     6901 tgaaaaacgt cgaggacagc gtattgctgt ttgtcgattt gggcttcggc aaagcgcgta
     6961 tgggcggctc tgcattcggt caggtataca acaacatgag cggcgacgcg cccgatttgg
     7021 acgacacagg ccgtctgaaa gcgttttaca acgtgattca acagcttgtc gccgaagaca
     7081 aactcttggc ataccacgac cgcagcgacg gcggcttgtt tgccgttttg gtagaaatgg
     7141 cgtttgcagg gcggtgcggc ttggatatag atttaaattt attgcttgca caaacattta
     7201 ttaccaacca taccgctctg tctcaatcat tgcggactga agaggtaaaa gcgttggctg
     7261 aatggcaaga aaccattgcc cgcacattat ttaatgaaga gttgggtgct gttatccaag
     7321 ttagaaaaca agatgttgcc gatattatca atttattcta tcaacaacag ctgcatcata
     7381 atgtctttga aatcggtacg ttaactgatg agaacacgtt aatcatccgc gacgggcaaa
     7441 cgcaccttat ttctgacaac ctaatcaaac tgcaacaaac ctggcaagaa accagccatc
     7501 aaatccaacg cctgcgcgac aaccctgcct gcgccgacag cgagttcgca ctgattggcg
     7561 acaacgaacg cagcgcattg tttgccaacc tgaaattcga cgtgaacgaa gacatcgccg
     7621 cgccgtttat caacagcggc gcgaaaccca aaatcgccat cctgcgcgaa cagggcgtga
     7681 acgggcaaat cgaaatggcc gccgcgttca cccgtgccgg attcgatgcc tacgacgtgc
     7741 atatgtccga cctgatggca ggccgcttcc gccttgccga cttcaaaatg ctggcggcgt
     7801 gcggcggctt cagctacggc gacgtactcg gcgcgggcga aggctgggcg aaatccatcc
     7861 tgttccaccc tgctctgcgc gaccagtttg ccgcattctt cgccgacccg gacacgctga
     7921 cattgggcgt gtgcaacggc tgccaaatgg tcagcaacct cgccgagatt atcccgggtg
     7981 cagcagaggc atggcctaaa ttcaaacgca acctgagcga acagtttgaa gcacgcctga
     8041 gtatggttca tgtccccaaa tccgcgtcgc tgattctgaa cgaaatgcaa ggctccagcc
     8101 tgcccgtcgt cgtcagccac ggcgaaggcc gcgccgactt cgcgcttcac ggcggcaata
     8161 tttccgccga tttgggcatt gcgctgcaat atgtggacgg tcaaaaccaa gttacccaaa
     8221 cctatccgct caaccccaac ggctcgccgc aaggcatcgc cggcattacc aacgccgacg
     8281 gccgcgtgac catcatgatg ccccaccccg aacgcgtgta ccgtgccgca caaatgagct
     8341 ggaaaccaga agactggacg gaattgtccg gctggtaccg cctctttgcc ggcgcacgta
     8401 aagccttggg ctaaccgccc tactcaaacc aatgccgtct gaagaatatt tcagacggcg
     8461 ttccggcata ccatccttta aacggtatcc gtccaccgag gaacactcat gaaaatcacc
     8521 cccgtcaaag ccctaaccga caactacatc tggatgatac agcacggcaa ccatgccgtc
     8581 tgcgtcgacc cttccgaacc ctcgcccgtc ttggaattcc tcgtccgcaa ccgcctcatg
     8641 cttgcccaaa catgggtaac gcacccccat cccgaccacg agggcggtgc ggcggcactc
     8701 tggcgcggct acatggaatc gcccgtttac ggcgaatccg acatcgaagc agcaacccac
     8761 accgtaaccg ccggcaccca attcaccttc ggcgacggac aggttaccgt ttgggcaaca
     8821 cccggccaca cagaccgcca caccagctac cttctcgaaa cttcagacgg catacacgtc
     8881 ttttgcggcg acaccctttt ttccgccggc tgcggacgcg tgtttaccgg cacaatcgaa
     8941 cagctttacg acagcttcca acgcttcaac cgcctgcctg aaaacaccct gttctacccg
     9001 gcacacgaat acaccgccgc caacctgcgt ttcgccgccc atatcgagcc ggacaacgcc
     9061 gacattcaga cggcattgaa agcggcagaa cgcacgccca ccctgcccgt taccctcgcc
     9121 cacgaacgcc gcgtcaaccc gttcttacgg acagaaatcc ccgccgtccg ccaacgtgcc
     9181 gaagccctgg tcggcaaaac gctgaacagc ggtttggaag tattcgccgc cctgcgcgaa
     9241 ctgaaaaacg cctactgctg acctgccctc cgaaaaatgc cgtctgaaac ccgcgtttca
     9301 gacggcattt gcgttaaaaa tagtaaaccg tttcaaaagg gagtagaata gtgccgtttc
     9361 caaccctgcg cctgtaccgt caggctttta ttatggacct tcccagttcg tttttactga
     9421 acaccccttc cgattccaaa cgacaataac catcccgccg gaatgcctcc ccgcgcacgg
     9481 cgggcggagc atttatgagc atcgaaccaa cccctccgaa ccttgaaaac gacggtatcg
     9541 aaaacgatgt agaacgcgtt tccgccgatt tcgaccgtgt ccactccctc tgcgaaatcc
     9601 tcgaacctgc ttttgaacaa atcgaaaacg gcacaccgct cgaagacgcg ccgctgcgcg
     9661 acaagctgac cgagctgacc gtcctcttgg gcgagctgca ccctgccgac gtggcggcgg
     9721 tgttggaatc gctgccgccg cgcgaacgca acgtcgtttg gcttctggtc aaacccgaag
     9781 acgacggcga agtattgctg gaagtatccg acgcggtgcg cgaaacactg atcgagtcga
     9841 tggataagga cgaattgttg gcggcagttg acgatttgga cgcggacgaa ttggcggaac
     9901 tggcagacga tttgccgcac caagtggttt acgaagcctt acagacgcgt gatgaggaag
     9961 aacgcgccca agtcaaggcg gcaatgtcct acgaagacaa ccaagtcggt gcgattatgg
    10021 acttcgagtt ggtcagcatc cgcgccgatg tcgcctgcga agtggtgctg cgttacctgc
    10081 gccgcttcga cagcctgccc gaccacacgg ataaaatctt cgtggttgac gaaaacgatg
    10141 tgttgcaggg cgtgctgccc atccgcaaac ttttggtcgc cgatcccgaa gacttggtgg
    10201 aaaacgtgat ggcgaaagat gtcgtgcgtt tccgcgccga agacgacgtg gaagaagcag
    10261 cgcaggcgtt tgaacgctat gacttggtta ccgcgcccgt cgtcgatgaa aacaaaaagc
    10321 tcatcggcag ggtgaccgtt gacgaaatgg tggacgtgat ccgcgaagaa tccgaagcag
    10381 atatgttgaa catggcgggt ttgcaagaag aggaagactt attcgccccc gtcttggatt
    10441 ccgtcaaaaa ccgctggatg tggctcgccg tcaacctctg caccgccttc cttgccagcc
    10501 gcgtcatcgg cgcgtttgaa ggcagcatcg aaaaaatcgt cgcactcgcc gcgctgatgc
    10561 ccatcgtcgc cggtatcggc ggcaactcgg gcaaccagac gattaccatg attgtccgcg
    10621 ccatggcgat ggggcaactg acggatatgc aggcggggcg tttgctgaaa aaagaagtcg
    10681 gcgtcgcctt ggtcaacggc atcatttggg gaacggtcat gggcgcagta tcttggctgc
    10741 tttacggcag cctcggcatc ggtttggtca tggttgccgc gatgacgctc aacctgctgc
    10801 tggcggcaac cgtcggcgta ttaattcccg tggtaatgga aaagttcgga cgcgatcccg
    10861 cgctgggcag ctcggtgctg attacggcgg taacggattc cggcggcttc ctgattttct
    10921 tgggtctcgc caccctgttc ctgctttaaa tgccgtctga acccgcgcaa aaatgccgtc
    10981 tgaagcggaa gctgcttcag acggcatttg actatttatc cttgttgcac aagattattg
    11041 gacggtatgc cggggcagcc ctttggcaac gccgaccaca tcctccccga acagcgcgtt
    11101 gacatcggtt tcgtcaaaca catatttgct gtggcagaaa tcgcaatcga cttcgatgct
    11161 gccttgttcc accaccacgc cgccgacttc ttccccgccc agcatcagca gcatatcgct
    11221 gactttgccg cgcgaacagg tgcatgaaaa ttcaaacgtt tccggctcga acacgcgcgg
    11281 cggcgtttcg tggaacaggc ggtacaaaac atgttgcgca tccaattctg ccagctcgtc
    11341 cgccgtcagt gtacgcgcca gcgtactgac gtgttcccat gcctcttcat ccaacaccgc
    11401 ttcaggcaga cgctgcacca gcagaccgcc cgccgcttcg tcgcttgcag acaggacgat
    11461 gtgcgtatcg atttgttcgg aacgcttcat atagttcgcc aacatttgcg cgataccgcc
    11521 gccttccaaa ggcactacgc cctgccaggg ttcgccgtct ttgggctgca gcgtcagcac
    11581 gaatacgccg ccctcgccca aaaggtcgcc gaggctttcg tcatcggcta tttctgcggt
    11641 ttcgtcccaa cgcgcggttg cacggacggt acggtcggaa gccgcttccg caaccagcat
    11701 tttcagccgc ccccgcccct gaacctgcac aatcagcgtg ccttcgtttt tgaggttgcc
    11761 cgacagcaac acacccgccg ccaacaactc acccaaagcg cggcggatgg cggcgggata
    11821 gtttttctgt tttacaatgt gctgccacac gttttccaga cggacgtgca gcccgcgcac
    11881 gggcatatcg tcgaagataa agcgggtacg cacatcggcg cggttgatgg cggtttgatt
    11941 catgattttc tctgactgat tgttcggatg gcggctatat ggttgcggtc ggcgcgaaaa
    12001 caagacggac ggcggatgcg cttcccaaat tatcaataaa ttatataaaa atcaacatat
    12061 taactcaatc taacaagccg ttttttgcca aacagccgtt tttttatata caatcaacaa
    12121 gatattttcg actgatacag cataacatcg cacggcggca cgatgcctcc tgcgcggaaa
    12181 caccgatatg gattcttttt tcaaaccggc agtttgggcg gttttgtggc tgatgtttgc
    12241 cgtccgcccc gcccttgccg acgagttgac caacctgctc agcagccgcg agcagattct
    12301 cagacagttt gccgaagacg aacagcccgt tttacccatc aaccgagccc ccgcccggcg
    12361 ggcgggcaat gccgacgaac tcatcggcag cgcgatgggg cttaacgaac agcccgtttt
    12421 acccgtcaac cgagtccccg cccggcgggc gggcaatgcc gacgaactca tcggcaacgc
    12481 gatggggctt aacgaacagc ccgttttacc cgtcaaccga gcccccgccc ggcgggcggg
    12541 caatgccgac gaactcatcg gcaacgcgat gggacttttg ggtattgcct accgctacgg
    12601 cggcacatcg gtttctaccg gttttgactg cagcggcttc atgcagcaca tcttcaaacg
    12661 cgccatgggc atcaacctgc cgcgcacgtc ggcagaacag gcacggatgg gtacgccggt
    12721 tgcccgaagc gaattgcagc ccggagatat ggtgtttttc cgcacgctcg gcggcagccg
    12781 catttcccat gtcggacttt atatcggcaa caaccgcttc atccacgcgc cgcgcacggg
    12841 gaaaaatatc gaaatcacca gcctgagcca caaatattgg agcggcaaat acgcgttcgc
    12901 ccgccgggtc aagaaaaacg acccgtcccg ctttctgaac tgatttccca aggaatacgc
    12961 aatgagtatg cccgaaatgc ccaaatggta cgacgatgac ggacagatcg tgtcctgtac
    13021 cgaaaaggtc aaagtgatgt ccgaaaatat ggccgagctg tatcagacgg cacaagacgc
    13081 gtttgaagac gcgctgctga tgggttgcgg cgaacgtcag ttgcgcgatt acctgctcgc
    13141 gctgattgaa ggtttggaaa atccctaccg caaagtctga acacgccccg gttgctgcgg
    13201 cacggtttat ccgtgccgtt tttgcgtttg tgcgcggctt cggcttttca gacggcatat
    13261 ttgacgttat gattaaacag ttaacaagat ttatcacaac gccgtcaaaa gacagacaca
    13321 caacatgaac atcatccgcg cgctcctcat catcctcggc tgcctcgcca ccggcgaaac
    13381 cgccgttttc ctagcaggca tcaaactgcc cggcagcatc gtcggcatgg gcgtgctgtt
    13441 tgcgcttttg caggcgggtt gggtcaaaac gtcttggctg caacagctta ccgacgcgct
    13501 gatgtcgaac ctgacgctgt tcctcgtgcc gccctgcgtg gcggtcatca gctatttgga
    13561 tttgattgcc gacgattggt tttcgatact ggtttccgcc tccgccagca ctttgtgcgt
    13621 actgctggtt acgggcaaag tccaccggtg gatacggggt attatccgat gaacgaaatc
    13681 ctcaggcagc ccagcgttct gcttttcctc acgcttgccg tgtacgcgct tgcgattatc
    13741 gtgcgcacgc gcacgggcaa tatcttctgc aaccccgtac tcgtcagcac tatcgtgctg
    13801 attgcctacc tgaaaatcct cggtatcgat tatgcggtgt accacaacgc cgcgcaattc
    13861 attgattttt ggctgaaacc cgccgtcgtc gtgcttgccg tgccgctcta ccaaaaccgc
    13921 cgtaaaatct tcaaccagtg gctgcccgtc atcgtttcac agcttgcggg cagcgttacg
    13981 ggcattgtta cagggatgta ttttgccaaa tggctgggcg cggaacgcga agtcgtcctc
    14041 tcgctcgcgt ccaaatctgt taccaacccc atcgctattg aaatcacccg ctccatcggc
    14101 ggcattcccg ccattaccgc cgccaccgtc atcattgccg gtctggtcgg acagattgcc
    14161 ggttacaaaa tgctgaagaa cacggtcgtc atgccctcgt ccgtgggtat gtcgctcggc
    14221 acggcttcgc acgcgatggg gattgccgcc tcgctcgaac gcagccgccg tatggcggca
    14281 tacgcggggc tggggctgac gttcaacggc gtactgaccg cgctgattgc gccgctgctc
    14341 atccccgttt tgggattttg aacccgtttc agacggcatt tcagcccatg ctgtctgaac
    14401 gccgacacac tcgcaaggag aaccgttatg gctgtcaacc tgaccgaaaa aaccgccgaa
    14461 caactgcccg acatcgacgg cattgccctc tacaccgccc aagcaggcgt gaagaagccc
    14521 gggcataccg acctgacact gattgccgta gccgccggca gcaccgtcgg tgcagtcttc
    14581 acgaccaacc gtttctgtgc cgcgcccgtc cacatcgcca aatcgcacct tttcgacgaa
    14641 gacggcgtgc gcgccctcgt catcaacacg ggcaacgcca acgcgggtac gggcgcacag
    14701 ggcagaatcg atgctttggc agtgtgtgcc gccgccgccc ggcaaatcgg ctgcaaaccg
    14761 aaccaggtgc tgcccttctc caccggcgtg attctcgaac cgctgcccgc agacaaaatc
    14821 atcgccgccc tgcccaaaat gcagcctgcc ttctggaacg aagcggcacg cgccatcatg
    14881 accaccgaca ccgtgcccaa agccgcctcg cgcgaaggca aggtcggcga caaacacacc
    14941 gtccgcgcca cgggcatcgc caaaggctcg ggcatgattc atcccaatat ggcgaccatg
    15001 ctcggtttca tcgccaccga tgccaaagtt tcccaacccg tcctccaact gatgacgcag
    15061 gaaatcgccg acgaaacctt caacaccatc accgttgacg gcgacaccag caccaacgac
    15121 agcttcgtca tcatcgccac cggcaaaaac agccaaagcg aaatcgacaa catcgccgac
    15181 ccgcgttacg cccaactcaa agaattgttg tgcagcctcg cgctcgaact cgcccaagcc
    15241 atcgtccgcg acggcgaagg tgcgaccaag ttcatcaccg tccgcgtcga aaacgccaaa
    15301 acccgcgacg aagcccgcca agccgcctac gccgtggcac gttcgccgct ggtcaaaacc
    15361 gcctttttcg cctccgaccc caacctcggc aggctgctcg ccgccatcgg ttatgccggc
    15421 gttgccgacc tcgataccga cctcgtggaa atgtatctcg acgatatttt ggttgccgaa
    15481 cacggcggac gcgccgcaag ctacaccgaa gcacaagggc aggcggtaat gtcgaaggcc
    15541 gaaatcaccg tccgcatcaa gctgcatcgc ggacaagccg ccgccaccgt ctatacctgc
    15601 gacctgtcgc acggatacgt ttccatcaac gccgactacc gttcctgacc cgacacggct
    15661 tcagacggca tacatatagt ggattaacaa aaatcaggac aaggcgacga agccgcagac
    15721 agtacaaata gtacggcaag acgaggcaac gctgtactgg tttttgttaa tccactataa
    15781 aatgccgtct gaaccgccgg acaacatacc atgacctcca cattcccccg ccgcctcgcc
    15841 cgcaaaatcc gccaaacccg ccgcctgtcg cgcaaaagca tcgcctttct gttccttttg
    15901 gcaggttcgg cactcgtcgc cctgaccgcg ctgttttttg cccatcttgc cgattttgcg
    15961 ctggaactga acgccaaact ggttcaacaa tacccgtggt tcgcgtgggt cgcgcttcct
    16021 ttgggtttac cgcttattgc gtggctcaca cgcaaattcg cccccttcac cgccggcagc
    16081 ggcatcccgc aggtcatcgc ctcactgtcg ctgccctacg gcgcacagaa aacgcggctg
    16141 atccgcctcg ggcagacgct gctgaagatt ccgctgacct ttttgggtat gctgttcggc
    16201 gcgtccatcg gacgcgaagg tccgtccgtg caggtcggcg cggcagtgat gggcgcgtgg
    16261 ggcgcgtggt gcaagaaaca cggcttggca ttcaaaggga tgcaggaaaa cgatttgatg
    16321 gcggcgggcg cggcgggcgg tttggcagcc gcgttcaacg cgccgctggc gggcgtgatt
    16381 ttcgccattg aggaactcgg gcgcggcatc atgttgcgct gggagaggca aattcttttg
    16441 ggcgtgctcg cctccggttt catacaggtc gccattcagg gcaacaaccc gtatttttcc
    16501 ggcttcaacg gcggcgtatt ggaacatatc tttctgtggg tcgcactgtc cggcctggtt
    16561 tgcggcgcgg cgggcgggct gttcggacgt ttgctctatc gcggtgcggc ggcgtttgca
    16621 ccgcgcaaga tacgcggctt catccgcaac cgtccgctgc tgctggcggc actgatgggg
    16681 ctgctgctcg ccctgctcgg cacgttctac caaggcaaaa cctacggcac cggctaccac
    16741 gaagccgccc aagccctgca cggcatctac gaagccccct tcggactcgc cgccgccaaa
    16801 tggctcgcca ccgtattcag ctattgggca ggcattccgg gcggcatttt cactccctcg
    16861 ctgaccatag gcgcggtttt gggcgagcat atcgccgcca tcgccgacat atcgcagggt
    16921 gcaaacatca tcgtcctcat ctgcatggcg gcatttctgg cgggcgcgac acaatccccg
    16981 atcacttccg ccgtcgtcgt catggaaatg acgggcggac aaagcctgct gttttggatg
    17041 ctaattgcct gcattttcgc ctcgcaggtt tcgcgccagt tttcgccgcg tccgttctac
    17101 cacgcatcgg gaatgcgctt ccgccagcgc gtgcttcaag aaaccgccgc ccaaaccggc
    17161 aatgcgcccg caagaccgca aacagcaaac agcaaaacgg gaatgccgtc tgaaaattaa
    17221 aacgcccccg atcaaacgcc ggcagccgcc ttgatttgaa taccgttccg ccgccgcttg
    17281 aaacttcagc aacaatgccg tctgaacgac agaatgcggt tttcagacgg catttcccca
    17341 tcccgatatt gcccgaaccc aaccaaagcg tttgctataa ttctattttt taccgcatac
    17401 gcaccaatca tgtttcccga tttctcccaa accctctcca aagaccgcca cttcctgcaa
    17461 tccgccttca aaaatcccaa caaatacggc ggcttgtcca aagtcgaaga aaaataccga
    17521 aaatcgcacg aaatcttttt gaagcgtttg gctgccttgc ccaaacccga attcgataac
    17581 accctgcccg ttcacgagaa gctcgacgaa atcaaaaaag ccattgccga gaatcaggta
    17641 acgattattt gcggcgaaac cggttcgggc aagaccactc agttgcccaa gatttgtctg
    17701 gaactcgggc gcggggcggc gggtttgatc gggcataccc agccgcgccg tttggcggcg
    17761 cgttccgtag cagagcggat tgccgaagag ctgaaatccg aaatcggcag cgcggtcggc
    17821 tataaagtgc gcttcaccga ccacacctcg cgcgatgcct gcgtcaagct gatgaccgac
    17881 ggcatcctgc tggcggaaac ccagaccgac cgttatctcg ccgcctacga cacgattatc
    17941 atcgacgaag cgcacgagcg cagcctgaac atcgacttcc ttttgggcta tttgaaacaa
    18001 ctcctgccgc gccgccccga tttgaaagtc atcatcacct cggcaacgat agacgcagaa
    18061 cgcttctccc gacacttcaa cggcgcgccc gttttagaag tgagcggacg gacgtatccc
    18121 gtcgaaatcc tctaccgacc gctgaccggc aaagacgaag acgacgcaga agtggagttg
    18181 accgacgcga ttgtcgatgc ggcggacgaa ttagcgcgac acggcgaagg cgatattttg
    18241 gtattcctgc cgggcgagcg cgaaatccgc gaaactgccg aagccctgcg caaatccacg
    18301 ctgcgccgca acgacgaaat cctgcccctg ttcgcacgcc tgtcgcacgc cgagcagcac
    18361 aaaatcttcc acccctcagg cgcgaaacgc cgcatcgtat tggcaaccaa cgtcgccgaa
    18421 acctcgctta ccgtgccggg catcaaatac gtcatcgaca ccggcctcgc gcgtgttaaa
    18481 cgctattccg cacgggcgaa agtggagcag cttcatatcg aaaaaatctc ccaagccgcc
    18541 gcccgccaac gatccggccg ctgcggacgc gtctccgcag gcgtgtgtat ccgactgttt
    18601 tcagaagaag attttaacag ccgccccgaa tttaccgacc ccgaaatcgt ccgcagcaac
    18661 ctcgccgccg tcatcctgcg catggcagca ttgaaactcg gcgatgtggc ggcattcccg
    18721 tttttagaaa tgcccgattc acggtatatc aatgacggtt ttcaggtgtt gttggagttg
    18781 ggggcggtgg aggccgtctg aaaacaggca gacataaaag aaaatccgcg tagagtgatg
    18841 taaacttacc cttgctttaa taagtagaaa atggtgggtt tacgtccccc cctgcggcta
    18901 ctaaaaaaat ataagagtaa acaacctttt tgaaagaaaa atgtatggac gaaattcaaa
    18961 tacccaaaaa agtggaatta caaaccaaac tagaaaatga aaagattgtt ttatcgaaag
    19021 gttctaccac gattattgtt ggtgctaatg gcacagggaa aacaagatta gctgtttata
    19081 ttgaagaaca attaaaggaa aaagcacaca gaatttcggc tcatagagca ttaaaattaa
    19141 accctaatgt caataaaata ccagaaaaga gtgccaaaac atatctatct tatggtcaga
    19201 actgggatgg aatcgatgta tcaaatagaa aaaattatag atgggataat aactcatata
    19261 ctcatttact caacgatttt gattggttat tacaatattt attcgctcaa caaaataata
    19321 ttgcggtagc aaataatcaa aagctcaacc gtaatgaaaa agtaaccaat tcaaaaacaa
    19381 agctagatat tttgcaagaa gcatgggaaa cattattacc acacagaaaa ttacatatta
    19441 cagcagatga tattcaagtc tctgctgtag ataatgagga attgtattct gcctcaaata
    19501 tgagtgatgg agagcgagca cttttctata ttcttggaca agttttgtca gtagatgacg
    19561 gttctgtctt aatttttgat gagcctgaat tacatattca taaatcaatt atttcaaatc
    19621 tatgggataa aattgaagaa ttacgacctg attgttcatt tctaatcatt acacacgata
    19681 ttgaatttgc tgcaactcga gtagctaaaa aatatgttat cagaaattat tatccgaccc
    19741 ctgcttggga tatttctgaa gttcctgaaa gtaattttga tgaagaaaca ataacgatga
    19801 ttttaggtag ccgtaagcca atattatttg ttgagggcaa caataatagt ttagatattg
    19861 ctacttaccg ctattgttat cctgattgga ccatcatacc caaaggggca tgcaaagatg
    19921 tcattcaatc agtatcatcg ctgaaaaaat taagtaatga aatgccatta ctaaacttaa
    19981 aatgttcagg tattgtcgat ttagatagta gggatgaaag agaaattgaa caattaaata
    20041 atttgggtat ttacatttta cctgtatccg aaattgaaaa tctttttagc ttaactgatg
    20101 tagcaaaaga gatattgaaa ctaaatcaat attcagatga agaattactc aataaactta
    20161 atggatttaa atccgaacta attaaatata tagataatga attaaaagac gataaattag
    20221 acgaatttgt tgtaaaacag gttcgacgta aaattgataa ttatttaaaa aatattgatt
    20281 tatcctccaa aataacaagt actgatatga aaaaatcatt acttaatgaa atttctactt
    20341 taacagaaca gaaaattgaa acatggattt cagaaattaa aaatgaaatt caaagatgta
    20401 ttgaacagca agatttggat aaattactta ctatatatga taataaagga ctcttggcta
    20461 aatcagcttg tgttttaaaa ggaatgcgta acaaacatga atttgaaagc tggataatga
    20521 gaacattaaa aggaaggaat aaagatttta ttgatgcaat cagacagaaa cttccaattc
    20581 tggattaaat aaaaccatct gaaaatttac cttcagatac agatatattt catgaaaaat
    20641 catcaaacta cactctcttt ccctacttcg agtagcctga aaccttgcgc agacaaacaa
    20701 ggcctgtctg aagaccgcag ccaataccgc ctgaccaaac tcggcgaaca aatggcgcac
    20761 ctgcctatcg acccgaaaat tgcgcgtatt ttgttagtat tattccgttt ttaaaaatgc
    20821 ccgattcgcg gtatatcaat gacggttttc aggtattgct ggaattgggg gcggtggagg
    20881 ccgtctgaaa ataaaatctt tctttataaa aaggcaggcc atgtttcatt ttcagacggc
    20941 ctaaatcatt gagaaactaa aaactattaa aaagggaata ttgggtttta aaactcaatc
    21001 ggtaaatttt tattgtgaaa tattaatgat gaaaaaatct ttccttacgc ttgttctgta
    21061 ttcgtcttta cttaccgcca gcgaaatcgc ctatcgcttt gtattcggaa ttgaaacctt
    21121 accggcggca aaaattgcgg aaacgtttgc gctgacattt gtgattgctg cgctgtatct
    21181 gtttgcgcgt tataaggtga cgcgtttgtt gattgcggtg ttttttgcgt tcagcattat
    21241 tgccaacaat gtacattatg cggtttatca aagctggatg acgggcatca attattggct
    21301 gatgctgaaa gagattaccg aagtcggcag tgcgggcgcg tcgatgttgg ataagttgtg
    21361 gctgcctgcg ttgtggggcg tgttggaagt catgttgttt tgcagccttg ccaagttcca
    21421 ccgtaagacg catttttctg ccgatatact gtttgccttc ctaatgctga tgattttcgt
    21481 gcgttcgttc gacacgaaac aagagcacgg tatttcgccc aaaccgacat acagccgcat
    21541 caaagccaat tatttcagct tcggttattt tgtcggacgc gtgttgccgt atcagttgtt
    21601 tgatttaagc aggattcccg cctttaagca gcctgctcca agcaaaatcg ggcagggcag
    21661 tgttcaaaat atcgtcctga ttatgggcga aagcgaaagc gcggcgcatt tgaagctgtt
    21721 tggctacgga cgcgaaactt cgccgttttt aacccggctg tcgcaagccg attttaagcc
    21781 gattgtgaaa caaagttatt ccgcaggctt tatgactgca gtgtccctgc ccagtttttt
    21841 caatgcgata ccgcacgcca acggcttgga acaaatcagc ggcggcgata ctaatatgtt
    21901 ccgcctcgcc aaagagcagg gctatgaaac gtatttttac agcgcacagg cggaaaacga
    21961 gatggcgatt ttgaacttaa tcggtaagaa atggatagac catctgattc agccgacgca
    22021 gcttggctac ggcaacggcg acaatatgcc cgatgagaag ctgctgccgc tgttcgacaa
    22081 aatcaatttg cagcagggca ggcattttat cgtgttgcac caacgcggtt cgcacgcccc
    22141 atacggcgca ttgttgcagc ctcaagataa agtattcggc gaagccgata ttgtggataa
    22201 gtacgacaac accatccaca aaaccgacca aatgattcaa accgtattcg agcagctgca
    22261 aaagcagcct gacggcaact ggctgtttgc ctatacctcc gatcatggcc agtatgttcg
    22321 ccaagatatc tacaatcaag gcacggtgca gcccgacagc tatctcgtgc cgctggtgtt
    22381 gtacagcccg gataaggccg tgcaacaggc tgccaaccag gcttttgcgc cttgcgagat
    22441 tgccttccat cagcagcttt caacgttcct gattcacacg ttgggctacg atatgccggt
    22501 ttcaggttgt cgcgaaggct cggtaacggg caacctgatt acgggtgatg caggcagctt
    22561 gaacattcgc gacggcaagg cggaatatgt ttatccgcaa tgagtggcgt aaaaaccaat
    22621 aaagacaaat ttagatgatg tcggggaaga tgcccgaccg acaagactat gcaaaatatg
    22681 aaaaaccaag tacgcggatc aggcatggat gcacgatcca atccggccaa tgtttcagac
    22741 ggcctgcaaa acagttcggg tcatatcggt accaacacgc gttaccgcct gaccaaactc
    22801 ggcgaacaaa tggcgcgcct gcccatcgac ccgaaaatcg cgcgcatttt gttggcggcg
    22861 aagaagcacg actgcatggc ggaaatattg gtgatcgcgt ccgcgctgtc gattcaagac
    22921 ccgcgcgagc ggccgctgga agcgcgcgat gccgccgcca aggcgcacga gcgttttacc
    22981 gacaagcaat ctgactttct tgcctatctg aacatttggg acagcttcca gcgcgagcgc
    23041 gacaagggct tgtccaacaa gcagttggtg cagtggtgcc gtcaatattt cctgtcgcac
    23101 ctgcggatgc gcgagtggcg cgagctgcac caccagcttg cccaaaccgc gattgaaatg
    23161 ggtttgacca ccaaggaagc ggctttcaga cgactttccg aaatcaagca gcttacgtcg
    23221 tctgaaaacc aaggcgatca agacttatcc gccaaacgca aacaaaaaca actggataag
    23281 aaacaacacc gcgcccaaat ccgcgccgcc aaagaagcgg gctacgaaca aatccaccgc
    23341 gccctgctca ccggccttat cgccaacgtc ggcatgaaat cgcccgacgg caacgactat
    23401 accggcgcgc gcggcagccg tttccacctt ttccccgcct ccgccctgtt caaagccaaa
    23461 cccaaatggg tgatggcggc agaactcgtt gaaacgacaa aactttatgc gcgcgacgtc
    23521 gccgttatcc agcccgaatg gattgagcag gaagccccgc acctcgtccg ctaccactat
    23581 ttcgagccgc actgggaaca aaaacgcggc gaagtcgtcg ccagcgaacg cgtgacgctt
    23641 tacggcctga ccgtattgcc acgccgcccc gtgccttacg gcaaagttgc gcccgaagaa
    23701 gcacgtgaaa tctttatccg cagcgcgttg gtggcgcagg aatgcgattt gaaagcggat
    23761 ttttttgtcc acaataaaaa gctgattaaa gaaattaccg aactcgaaca caaatcgcgc
    23821 aagcaagacg tattggtcga cgacgaagcc ctgtttgcgt tttataacga acgcctgccc
    23881 gaaatggctt ggaaagacgc gcaaggcagc gtttggggaa gtgaagattc cgtacggatt
    23941 attgaatctg acaaagccga gaggtcgtct gaaaatgagc gcaacgagtt tcgtaaaaac
    24001 aagcgtaatg ggtctcgcca aaatgaaaat cacggcaaca ccgtaggttg ggttgaaaac
    24061 ccaacatcag ccgcaactgc aaaaactgtt gggtttgaca acccaaccta cgctactcag
    24121 caacccaccc cctcccccgc gagggagggt cggggagagg gcaaaacagt tgccgcacaa
    24181 accaactttt ccgcaaccgc agcaaaccct ctcccgcagg agagggagca gagtgccgca
    24241 gcttcagcgg tttcaaacga cctgcatcct gcaaatcccc aacaaaccgc cccctccccc
    24301 gtgggggagg gtcggggaga gggcaaaaca gttgcctctc aaaccaactt ctccgcaacc
    24361 acagcaaacc ctctccctaa ccctctccca caggaggggg aacagagtgc cgcagcttca
    24421 gcggtttcaa acgaccctca accccaaaag cagccagcac cccaaaaaga ccgtctgaaa
    24481 cccctccccc tcgccgacat ccgcaccttc caagcctggc tcaaaaccgc cgaacgcgag
    24541 aacccgcgcc tgctgttcct cagccgcgac gatttgatgc aacacgccgc cgcacacatt
    24601 accgaagagc aattccccaa attctggcaa accgcagacg gcaagttcaa actttcctac
    24661 cgcttcgagc cgcaccatcc gctcgacggc gttaccctca ccctaccgct gaccgtcctc
    24721 aaccgcctgc acgcgccgtc gctcgaatgg ctggtgcccg gtatgttgcg cgaaaaaatc
    24781 cagctgctga ttaaagcact gcccaagcaa atccgccgca tttgcgtgcc cgtacccgat
    24841 ttcatcacca aatttctcga aagcaatccc gaccgccaag cggtcattat tccccagttg
    24901 gcgcacttta tcgccaaaag cgcaggcgat atgcggattt tcgagcaaat cgaccaagac
    24961 gcatgggcgg cgcaagaatt gcccgaacac tgctatctga atctgctcat catcgacgac
    25021 ggcggacaag agcttgccgg cggccgcaaa ctgcacgaat tgcaacaaca gctcggccaa
    25081 gccgccgccg tcaccttccg cgacaacacc caagaattcg agcgcgacaa cgttaccaca
    25141 tgggatatcg gtaccctgcc cgaatccatc aaattcgccc gcggcaaaca acagctcacc
    25201 ggctacctcg gtctgcaaaa agaaaaagac ggccgcatcg ccctgcgcct gtgtgacacc
    25261 accgaagctg ccgaacaggc acaccgtcaa ggcgtcatcg aattgatgaa gctgcaatta
    25321 aaagagcagg taaaagacct gaacaaaggc atccaaggct ttacccaagc cgccatgctg
    25381 ctcaaacaca tcaacgccga caccctgcgc gacgacctca cccaagccgt ttgtgaccgc
    25441 gcctttatcg gcgaagacga gctgccgcgc aacgaaaaag ccttcaaaga acaaatcaaa
    25501 cgcgcccgca gccgcctgcc cgccgtcaaa gaagccctca gccgctacct gcaggaaacc
    25561 gccgccgcct acgccgaact gaacggcaaa ctcggcaaac acccattgac ccaccttcta
    25621 agactacgcc tgcaaaccct gctcgccgcc ggcttcgcca cccgcacccc gtgggcacaa
    25681 tggccgcgcc tccccatcta cctcaaagcc atgaccctgc gcctcgaaaa atacagcagc
    25741 aaccccgccc gcgacgcagc ccgcgaagcc gatatacagg aattggaaca aatgtggcag
    25801 gaaaaaaacg acggtttggt gaaacaagga ctacccgttt cagacgacct caccgcgttt
    25861 aaatggatga tcgaagaatt gagggtgtcg ctgttcgcgc aggaattgaa gacaccgtat
    25921 ccggtgtcgg tgaagcggct gttgaaagag tgggaggatc ttaattaaag gaagatgccg
    25981 tctaattttt tcagacggca tcttttattt ccctgaccaa ccgatttaat cgctccctac
    26041 aaatagcttt attttttaat tcgcactccc aaacgacaat cactttgaaa ccgatctttt
    26101 ccagctcggt ttcattttta atatcacgtt cgcaattttt tgtgattttt tccaaccaaa
    26161 aatccgtatt gcttttcggg atatgtcctt tattacaaga atgcccgtac caaaaacagc
    26221 cgtgtataaa cacgactgtt ttatatttcg acaaaacgat atccggtttg cctacatagc
    26281 gtttatcgtt tttccgataa cggaaacctt ggaaaaaaag aaatttcctt accaatactt
    26341 ccggtttggt ttctttgcta tggatttttg acatcacgaa agaacgtttg gatgtagtga
    26401 aaatatcggt cataaattac ttggaagata ccaccttcga tcgttttttt gtctgttcca
    26461 cttttcttaa caaatacgaa accaagctta ttaaatccaa agcatcctta tcattaaaaa
    26521 tactaggaga taaatttgct cttaactcat gttgtacagg atttctaaat ccagtccatg
    26581 ttccgcatgc aagctgttca agaccatctt ctaaattttg ttccgccttc gttttgttat
    26641 tcgttaatag aagtatagaa tttttactcc cgaaagcttg ttcaatcaat tctcttccgt
    26701 ctatgctatg aagacctgtt tgcttctgaa ccttatcttc gaaaacttta actgcctcga
    26761 ttgcagcact taaataatcc ttcttttcat tgtaaaaatc attacaggca gtatgcaaat
    26821 cctgatgcaa atgccgccaa tgatagtaag gatattctgg aataatacta tgtaaggcag
    26881 agattacttc cgcttgctct ttatttgtaa gctccgaatc ttccaaacga ttcaaaataa
    26941 cttctactgg ttccctttcc ttttctggca atttgttttt ccaatcttcg atatcaactc
    27001 cgctttcgcc tttcacttcg tcatctttct ctttttttct tctttttctc caatcttgcc
    27061 cgatggctcg gacgatctcc ccaaggaaaa cacgtaattt tgaggttttt gaatgcttcc
    27121 agtctaatga tgtcctgtcg gtagcaatta aatcttcatc gtcattatct ataaagtcca
    27181 cattcagcca tccagtcaaa tatgaataga aatggctgga ttcgctatca gtgaaaaact
    27241 cgggcatatt taccattctg ccgttggcaa acaaggtaat acctttcata ttattcttta
    27301 aaggtttttc cgttgtaatg aacttacctt gtattaatcc gctgtattca tactgttcga
    27361 attttgaaga taacgatgaa atattggttg ctaaatcctg gtattcccat tcgaattgtg
    27421 gggtaacaag atcatatttt agatttccat caatgatttt aggttcagag ccgttcaagc
    27481 ttactttgat tttaaaatcc tgagccggaa aatcaaacaa gcgggaaaga tgctctacat
    27541 aattatctaa cggatatcct tgctttttcg tcagttcggt taaagttatg gttgtcccgc
    27601 tttcgatatt ggattcaaca gattcttttc gaaactccgg ttgataaata cccttgcttc
    27661 ttcgaatctc tgcataatcc aaagtaaaag taaccctttc gtttccctgg atagtagaaa
    27721 tttcaatttt gttgccaagc ccgaataatg ccaatttacc aaggcctttt ttacccgttg
    27781 gaattcttcc gcacggggag gcttgttttt cttcccttct gttccgaccg attctcaaat
    27841 aaaaatcatt gatttcatcg aagctcattc ctataccatt atctttaata acgattttat
    27901 gctccggttt atcgaataac ctaacttcca cttccgtagc acatgcatcg taggcattcg
    27961 ctatcaactc ggcaatcgca ggaacagtgt gcgaatacat ctttacaccc aaatgttcga
    28021 ttacggttgg ctcgaaactc attttcaatt tttcttcaga catgttttct ccttttctaa
    28081 cattcgatat tatcaacaat tgccctgcct attttttccg catacatcgg aggaacggca
    28141 ttaccaatca aacgggctat cttgtccctg ctgcccgctt taaagacata gttacgaggg
    28201 aacgattgca atgttgcacc ttcgcgcagt gataacgccc tatcttcttc aggatgggca
    28261 aacctcccgt tggaaatact aaagaatttg gtcgttatgg taggtgcggg cttatcccac
    28321 catagcctac caaacgtgtc tttaaaacta ttgtcttttc cgataaaaca ttccagctgt
    28381 aattcgggaa tatgcgcaaa agccaaacgg tttcctccgt ttttaggtat caaagccaat
    28441 cgtttcaagt tgatatcaga taatcccgca cagctatgca taaaatccgt ttcgtcttgg
    28501 tgtcctgcaa taattttggg aaagccattt tccattccca aaacatcgcg taccgtaagc
    28561 cgtttgcccg aatacttgac tggttccagc ttttctttgg ttattctgtt tgcaattaac
    28621 gtaaatcttt tacggctttg gggaattccg taatcagccg tattgtgaat accgaaatgg
    28681 acggtaaatc ctttttcttc caataacttt atgaaattat caagcctgct ttctttcatt
    28741 ctgctcagaa ttcccggtac gttctctaca acaacatatc cgggattgaa atattctaca
    28801 aaccgctgaa actccaaaag caaactcttg gatttttccg attttctttt gtctgtttga
    28861 ataacactcc aatattgaca aggactacaa cctataagaa tcaaatcatc atcattcttt
    28921 tttaacccga gttctttttc caaggtttca ggctgcaatt caaaaacatc agcctgtata
    28981 aatttggcat ttatattggc ttcatatgtt tctttgcagc tgggttcgta atctatcccg
    29041 gctaaaacct gaataccggc ggattgcatt ccatagctca ttccacctcc gccacagaaa
    29101 aaatcaaccg cctttaactt actgtttttc atttctattt aaataattag actagtgaga
    29161 actaacttta tataacatat aatttattgc ttatcaaaaa taatttgaga ttttattaaa
    29221 attcatgcat ctaaataagc agaacttaat ttcctccaaa cgagcggatg tcttcaaatg
    29281 cacacttcac agaaaggtcg tctgaaaccc attttcagac gacctttttc cacttcccaa
    29341 aacaagaatt cccaccccct ccccaaacgt cgcccgaacc agtaaactcc ccatatttca
    29401 acaacttctt ggaagccaac catgccccgt atccaccctc ctcacctgtt cccgccccat
    29461 atcgccgaac gcggcctttt gtattttcag cagggcaagg ttcgcgatgt ccgaaaaact
    29521 tccgccgggc gttatcgggc ggaggtatgc ggtttggaaa actattgggt atggctgaag
    29581 ctggatagtg atttgtatat taaagacgaa agctgcgatt gcccttatgt ctaaaggtgc
    29641 aaacatacct taaattacta tattacatag gcaaaataca agcctataac gaattggaaa
    29701 caaaatgccg tctgaaaaca tcttcagacg gcattataaa atctgttcac cttttcagat
    29761 gagtaatgta cacccctata caatttttgc tactataccc cataaatcca cggctaaaga
    29821 tatccttatt atgtcctatg atttatcgaa acgacttgta atcggcttag catcaagtgc
    29881 cctattcgac ttatccgaat cggataatat atttagaatg gaaggggcag aaacctatag
    29941 gcaatatcag agagaaaaac aaaaccatcc cctaaaaaag gcgttgtctt tccatttatt
    30001 aaaaaacttc tgtcaatcaa tgaaataaac ccaaacgacc caacgattgg gtttattctt
    30061 ttatccagaa acaatccaga tacagattac gagtcataac tataggctta atattacacg
    30121 attttcattc catcaaggcg gaaaaccgca caaatactga aacactatcg atcgatttgt
    30181 aaacaagcct acttaagtaa cttgcagtcc ttatcatttc ctttaaaata atccagcccg
    30241 tcactacacg aactggcgga cttcttgcaa ataaaggtta ctagattttc attcatctta
    30301 ataataaaag gatttttatc tttatctatg gctaccgcct tcaacatgaa tttactgtct
    30361 aaggccccgc gcgcgattcc attcaaacgg atacaaaagc cttctgcctc tttaatcggc
    30421 aaacttggcc atttggtaga tgtttgttta aatctcccat tctgcagata aaacttttcc
    30481 ataaaatgtg cattttctaa caaggctgcc cgcactgtat ttatctttgc tttctcaaca
    30541 taattgcgat agctcggata aacaattaaa gcaagtacag acaatatcaa gaccactgat
    30601 attaattcaa ccagcgtaaa cccccgatta tcagtcatta ctttacttcc aataagaaca
    30661 gattattcaa catatttctt tgaacagact tactatccca ttcagcagta tgcatatttc
    30721 ccact
//
LOCUS       DAWKOG010000006        28871 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.006, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000006 DAWKOG010000000
VERSION     DAWKOG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 28871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28871
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.006"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..276
                     /locus_tag="WJK66_000211"
     CDS             <1..276
                     /locus_tag="WJK66_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7577984.1"
                     /translation="QKANEQEPFIAAVAETLSRTMWGYVMRMDARFEVTFIVDLGHVA
                     FEHQFTAVKQALKEKFEVEADGDALTVESDRLPYGIAACRVVFGDVL"
     gene            273..554
                     /locus_tag="WJK66_000212"
     CDS             273..554
                     /locus_tag="WJK66_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7577985.1"
                     /translation="MSGDTPITVEYVFGTLVSFLIALLWYWVKSISDGLKEARKDRDE
                     LLGRLHSVETSYQTKAEAKDNRNEILNLLREIKADLKEVGQKIERKADK"
     gene            554..769
                     /locus_tag="WJK66_000213"
     CDS             554..769
                     /locus_tag="WJK66_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7577986.1"
                     /translation="MQNQDPILRALAEINGKQDKLLQNQERMDAEIKKIHADCRRTSA
                     TTGAAAGAISGGIVATGIAFARAKLGL"
     gene            772..1278
                     /locus_tag="WJK66_000214"
     CDS             772..1278
                     /locus_tag="WJK66_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ7577987.1"
                     /translation="MAHPKATRDKLRALYCNGEQSLETAAALCGVSLGTARRWRDEAK
                     AQGDDWNKLRAAHTLAGGSIDEIARAMMTSFLVQYQATMTMLQDAEVEDLPPSKRVQL
                     LASLADAFTKTVAANKRVLPETSQLATALELLQFLMVFVQEKHPKHLAAFVEVLEPFG
                     AEVERKFG"
     gene            complement(1275..1469)
                     /locus_tag="WJK66_000215"
     CDS             complement(1275..1469)
                     /locus_tag="WJK66_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7577988.1"
                     /translation="MKSIDLEISKLLDAGKYTPSEIQDLLEEQGFKISLKKLADHLDL
                     LVAIGVAGKHSDDTFTSRLN"
     gene            1481..3100
                     /gene="terL"
                     /locus_tag="WJK66_000216"
     CDS             1481..3100
                     /gene="terL"
                     /locus_tag="WJK66_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEZ7577989.1"
                     /translation="MKNKDFLKSLSALAANLRQVIEAEVDGFDASPTAVAERRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSDLHRFLFRRLPEIVESPEGENEAVGAPRGEGKSTQ
                     VTQLFTLWCIVTGRKHYCVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFADVCGQGRV
                     WQAGTIVTANDIKVQVAGSGKKLRGLRHGPYRPDLTILDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWKGIKFKAMLKWPDRMDLW
                     DRWEELYRNEGELVADAFYQTNKAEMERGAETSWAARGVLALMKIRARDGHAAFDSEY
                     QNDPVSGEDAPFAQAMQFWAELPADLVYFGALDPSLGKAGASRDPSAIIVGGYQRSSG
                     KLFIVEAQIKKRLPDLIIEDVIRLHAQYKCKLWFVETVQFQEFLKDELVKRSAAKGIP
                     VPARAVKPIADKLLRIETLQPHMANGLILLHANQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGATANSAPVEYMAVTKHGTDGGFGSGAW"
     gene            3179..4738
                     /locus_tag="WJK66_000217"
     CDS             3179..4738
                     /locus_tag="WJK66_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HEZ7577990.1"
                     /translation="MKNIFSSALSKILPGRYTPKSTPQTAEITQNTQTHEHPSKGLTP
                     QKLHGILEAAERGDMKAQSELFADIEEKDGHIFSEMSKRKRAVIGLDWRVMPPPNSTD
                     AERRLAEEVKGWIERLPDFEDMMFDLLDAVGHGFACVEIEWQQIGGLWLPKNFIHRPQ
                     GWFKVDGADNVRLAKQDNPDGEELWAFGWLVHKHRSRSGLLVRGGLMRTLVWPYLFKN
                     YSVRDLAEFLEIYGLPTRLGKYAVGADETDKTTLLQAVKEIGHNAAGIIPETMNIELL
                     NAANGSSEPFMAMIDWADKTSSKAILGGTLTSMADGKTSTNALGQVHNEVRHDLLVSD
                     AKQLAGTITQQLILPLLRLNKGNVDETCLPRFQFDTQLPEDMAVYAESLPRLVEMGMK
                     IPLAWAQEKLAIPLASDDEPVLALQTAESEGVKVAPLSYRRVALSRQGEILDMGQAAI
                     DNAGLGKIALPEHIEPFLRGLGQALAEGDSYEEVQERLLRAYPHLDSAEFQTALARVI
                     FISDLWGRLNG"
     gene            4731..6077
                     /locus_tag="WJK66_000218"
     CDS             4731..6077
                     /locus_tag="WJK66_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEZ7577991.1"
                     /translation="MADLGFAFGLEPEAAVKYFEGLGYHIPPDWDVKWNEAQTKARTI
                     AGIHRQDIVGEFHAAMYEAAKSGKSFEAWRDEVQGRLKAHDWHLLKDGDIVDGDTGEV
                     LGRGITKHRMETIFRTQMQSAYMAGHWQAFEEGRDDAPWLQYSAILDSRTRQSHAAAH
                     GAVYHIDDPFWDYFYPPNGFNCRCTVRALSDSDLKRRNLLPQKAQLEDTEVVVNRKGD
                     TRPAKAVKLPDGRRFYTDAGFQHNVGKSHLANLGQLQMQRAVELPPKLASVSIQTALK
                     QPDLMRAVSQQAAQMVRRVDAEKVARGKTLYVGALALPVLDALAARKIYPQSAVIAIS
                     DERVLHALRDKKVKPLPVSFWEKIPELLQEPEQILLGKAGRNDDAKQFLAFVYPLSAG
                     KGKLVVTLDYDVKTRHPLTGKKEHLTLNMVNTGMIAETDKQVDSLLYGYETIWKKP"
     gene            6181..6678
                     /locus_tag="WJK66_000219"
     CDS             6181..6678
                     /locus_tag="WJK66_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HEZ7577992.1"
                     /translation="MIEIKINTDTLQNSLNAAARHTSHTKPLMTRLARIMRNAVLENF
                     AAGGRPAWAPRKYPTAREGSGLLQASGRLRNSITPSSTNDTAVVGTNVEYAAIHNFGG
                     KTSPHLIRPKKGKALKFGGRFAKQVNHPGSNIPARPFMTLQPEDEKALSDAVAEYLAQ
                     AIQGR"
     gene            6887..7951
                     /locus_tag="WJK66_000220"
     CDS             6887..7951
                     /locus_tag="WJK66_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ7577993.1"
                     /translation="MRAMDTKTLLAALSAAHVGGSDGLIKIVPKGQFAPVDGRTDTGV
                     PHWTMSADLAQQIIAAFDAAQTDLVVDYEHATLKAAETGQQNPAAGWISKYVWDDERG
                     LMGEVKWTQRAKDMIDSGEYRYLSPVLEYDTLGNVRGLHSVALTNSPALDGMALAALS
                     RQNSINPKQETSMNKEALIKLLGLAADADDKAIEAALAEAQEKLGGKTLAEALAAPKE
                     EPQGSEGDKGDAGKPEDKPQGGNADDGEVAELKAQVAALSKKVIAMEVGGTSDGLIRA
                     ALSDGRLLPHQEASARQLAAKDPEAFKALIDGSLALAALSKTQTGGKGGADGTSALTA
                     EEAAVAAQLGISAEDYAKAK"
     gene            7973..8875
                     /locus_tag="WJK66_000221"
     CDS             7973..8875
                     /locus_tag="WJK66_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HEZ7577994.1"
                     /translation="MIITPDTLKALFTGFKKNFQDGLKMAESQYKEIATVIPSSTASN
                     TYGWLGQWPAFREWVGDRVFQDMKAHGYAITNKHFESSVKVNRNDIEDDNVGIYAPMM
                     TEMGRASAVHPDELVFALLKNAHATLCYDGQNFFDTDHPVYEKVDGTGQASTVSNIFA
                     GSEAAWYLLDTTRALKPLIYQERKAKQFTAMTADTDEGVFMRNEYRYGVDGRCNVGLG
                     FWQMAAKSQEKLDAAGFEKAYNAMVSLKGDGGRPLAIRPNVLLVPPALENAAKELVEG
                     DRLANGAYNPNKGKAKVIVSPWLL"
     gene            8913..9386
                     /locus_tag="WJK66_000222"
     CDS             8913..9386
                     /locus_tag="WJK66_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7577995.1"
                     /translation="MAKEKNGQEVGATVDVQPEEVTVTAALQAEIEALRAELDKANAE
                     IQVAQAELAAAQERNAKLERLLEAGLAPGGISDGETLTPEAEAAFYTGSGAPAADAEV
                     VAIKSKHGHAFFRAGYHVQPNWTFVRRADFEPADFERLIGDRMVEAREALPTDAS"
     gene            9383..9808
                     /locus_tag="WJK66_000223"
     CDS             9383..9808
                     /locus_tag="WJK66_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 family protein"
                     /protein_id="HEZ7577996.1"
                     /translation="MSYAAVADLVARFGEATITGLTDLSRKGAVDETVAQQALDDAAA
                     EIDGYLMNRYTLPLPKPLRILTVYCCDIAVYRLCTGKRQLTEDIVHRYEAAVKFLQLV
                     AAGKVGLGVTEPAGEKPAVQGNGVMFTTQEKVFGRDSVY"
     gene            9792..10460
                     /locus_tag="WJK66_000224"
     CDS             9792..10460
                     /locus_tag="WJK66_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HEZ7577997.1"
                     /translation="MIASIEQAIRQRLSDGLGQMVTGVFTYGGEFDGEGLAQVVNQFP
                     AVWVMFAGIKDTIRHDTRGSRFKAIGQFTVLVGDRASGSEADSRFGGLHRHDVGTYRL
                     MQTCRHLLTNQSLGLQIDRLQPGAAKSLFSRQMEQDAVSVFALEFETYWFEDALQDGD
                     WPRPAVSDGQQAKVYADVSEYQGRTEPEHPDFKGVNLELRIPPKTPDQPADMAATVET
                     KVKP"
     gene            10457..10645
                     /locus_tag="WJK66_000225"
     CDS             10457..10645
                     /locus_tag="WJK66_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HEZ7577998.1"
                     /translation="MNETIKVRAADGLQVPLAGKPHEYITDQETVTVPDAAYYRRCIE
                     YGDLVVVTEDTQPAKGSK"
     gene            10645..12072
                     /locus_tag="WJK66_000226"
     CDS             10645..12072
                     /locus_tag="WJK66_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ7577999.1"
                     /translation="MASANINFEKIPASTRKPGVYAEWNTKLAVRNLPTNKQRVLIVA
                     QHNNPALGELTELENVFSAADAAAKYGAGSMAHLMVTAAIKAYAYADLSLITVADNKA
                     GVAAGGKITLSGTANTQGVLRVSIANADTLTIGIGAEDTAATVAAAVKAAIDAVPDLP
                     VTATVAEAVVTLTAKNKGTAGNAIRIKTSNTAEGITAAVTAMTGGDANPDIAAALNAV
                     VAEGHHIIACGINDETNLLKLRAHLDTVASPMEKRWAICVYGQTGTLAQATTLAGRLN
                     HGHIVSAWYRGTPSLPCELAAAFAAVMASEEDPARPLNTLALNSIGVCESKDKTMRTE
                     QENALYNGVTPIETSPAGTQAQIVRAITTYTKTANGTADESLLDVTTVRTLIYVSRAC
                     VDRIALRFPRDKLSDRTPPRVRSELIDVLMCCEELEILERVEENLPKLIVERDLQNTG
                     MLNCRIPSDVVNGLHVVGMVVDLYL"
     gene            12086..12460
                     /locus_tag="WJK66_000227"
     CDS             12086..12460
                     /locus_tag="WJK66_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ7578000.1"
                     /translation="MSTEYVGSVILYLGANEVEVTKIDVKDNTGKKPVKTMNRTRRVK
                     GFTRGVGQYDITFTAVVPTDGTAVDWAKIDDAKISLVPDVEGARPTSYLGFCATEAGD
                     SYTVDNELVVDVTGFAIRKVLE"
     gene            12636..13022
                     /locus_tag="WJK66_000228"
     CDS             12636..13022
                     /locus_tag="WJK66_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7578001.1"
                     /translation="MGLTKFGAAVREARRQTKQTLQTMAATLGTSPAFLSAIETGRSK
                     VPMDFVKKVEDFFENLGQPIDGLKQKAMVSNENVSLSGLSLQQQMLVAGFASSDFSKE
                     QLDKFAELLRTIHHKDSQKEGDDAAN"
     gene            13009..13473
                     /locus_tag="WJK66_000229"
     CDS             13009..13473
                     /locus_tag="WJK66_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEZ7578002.1"
                     /translation="MQQTNYSMRGVRVGMLSEETIQFFANEAAKFLVVDKRTRKKMDA
                     FMEMLEEYGIVIDVVADSEWLVITNAMCHNGTILMPNSLYTRICNGEDEAIFIFFHEL
                     GHLLLGHKAMLHYSEVLPTKQEDSEWQADEFAKCILRNMGIKYVPEQLSLRF"
     gene            13655..13834
                     /locus_tag="WJK66_000230"
     CDS             13655..13834
                     /locus_tag="WJK66_000230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578003.1"
                     /translation="MMATIFTIGGAIMAMTKAPVTPDLVDDTGAYIFRKSFTTKSGKK
                     IVSKNGKPFKIPVKV"
     gene            13963..14352
                     /locus_tag="WJK66_000231"
     CDS             13963..14352
                     /locus_tag="WJK66_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578004.1"
                     /translation="MSEFSPELTRAIEDYELRVSPDLKTVAGRLKYGIGVIDGEMHHD
                     FSMHLLTVREDMAIDPQLEGQPRLVAAYAASLDKLGGLTAESLTPDLLLDEMAAADFD
                     ALYWAQELLQKKRLCPHPAPIVTDTPS"
     gene            14310..14558
                     /locus_tag="WJK66_000232"
     CDS             14310..14558
                     /locus_tag="WJK66_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578005.1"
                     /translation="MPAPRADRYRYAVLKLGRYGITPAQIDGMTQPELEGWLKQADLI
                     ERGRAAPVVMPWFIPANVKPSAATGGQTQTFISKRKKK"
     gene            14555..16604
                     /locus_tag="WJK66_000233"
                     /pseudo
     CDS             14555..16604
                     /locus_tag="WJK66_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            16604..17971
                     /locus_tag="WJK66_000234"
     CDS             16604..17971
                     /locus_tag="WJK66_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HEZ7578006.1"
                     /translation="MAWKDTLLDAGFKGVTFDVIDDTLRGTHALAEHEYPFVQGADIE
                     DTGVSAMDMALTAVLWGEDYEGRLQNLLNVLCETGAGELIHPIYGSVPDCVVADFEVA
                     HNEENPDYCTVRMTFKQSVKAAPFFDRDLPIALADEVDFLADLAAWQGFEVFQTALNK
                     IQKAQSRWNAFHATVLMVVGVLYGQVNGIFTGGLNLLNSPRVLVAELKSVFGALANMH
                     NVGKSGLDGWRDMVGGVSKAAATPWQVSRGTEGSVAAVDLIQRAKPEDVAAFTALTAT
                     VGACALAEQAADILAVQLNEPTLTPVEISRLLADTRAALQRALAAQRILAMMLADETK
                     ADKLAYCLLRLYQTPVDSADDVYQRIEVAGLLPQAPYLETAAALTESLRNTAHKLQKQ
                     AFTVINMKPPLVQKTVTCDIGLHLLAFEWYGDYSRFGELLRLNPQIRHPNFIEKGTVL
                     NAYAR"
     gene            17955..19094
                     /locus_tag="WJK66_000235"
     CDS             17955..19094
                     /locus_tag="WJK66_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ7578007.1"
                     /translation="MPTPDNTVTLLINGKTHGQWTNYDIVSDLLTPADDFSVMLGRPV
                     DAKPDAVRAGDKVEVRVGGDTVLSGRIDRVQTVTEKGGKTLTIQGRDDAGVLLDCSAP
                     LFNAQDMDLNQIIEKIVKPLGLAKIRIDAAKTDKTHKVQIEPGSRAWDALLEYAEANG
                     LWPWLEPDGTLVVGGPDYTAAPVAELVLRTNGQNNNIKRLEVNRDMAARYSEVTVLAQ
                     SHSGKNNIKATAKDESVKLHRPLIVTEPDIDSQAQAQRKAKKRLADSRLEGLTITATV
                     QGHRTDDGTLWQPGQRINVLSEPDGIDAVYFLMARTFIGGRGQPTETVLTLKEDGAWV
                     LDADPPKKSGKTKRPSESRKANGHAAAKPKKRRQAKQAGQELQVI"
     gene            19095..19724
                     /locus_tag="WJK66_000236"
     CDS             19095..19724
                     /locus_tag="WJK66_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEZ7578008.1"
                     /translation="MDAKTIDNRIRRAFNGIRQAFRGKIARVKAAGGVQQIQVEGLEG
                     ETVQDLEHAENFGFTSNPPAGSDCVVVPLGGKTSHGIIVTTTNGAYRITGLAEGETAV
                     YNADGAKMVLKKGRIIEIDCETLNIKAPGGVNIDAPNVGCTAQITAEGQINGNGGMAV
                     KGGSGTSFTGNVQMVGDLDTTGKLTNNGKNVGSDHKHNGDSGGTTSDPL"
     gene            19785..20138
                     /locus_tag="WJK66_000237"
     CDS             19785..20138
                     /locus_tag="WJK66_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HEZ7578009.1"
                     /translation="MDALLNPQTGGYVVNQSAQSIENELYIRLVTPLGSYWADRTLGS
                     RLHELRRQKHLKRIEVLAKQYAEQALQPVIQSKRAQSIQVTASAPQHGWLKLHIEAVD
                     AAGDTVTLNHKVAVI"
     gene            20135..20620
                     /locus_tag="WJK66_000238"
     CDS             20135..20620
                     /locus_tag="WJK66_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ7578010.1"
                     /translation="MTQALNLEQIRANYLRDLQNQNPAAHVHAGSDNHVRATAIAAVG
                     EGQYQHQEWILRQAFADTADSAYLEKHAAKYGIYRKTATFAGGKVRVRGAVGATVPVG
                     QQINVGDKVYLTAESAVISALGSAEIAVIATVAGSAQNQTAETAATLQSVPAGIDSSA
                     V"
     gene            20623..21198
                     /locus_tag="WJK66_000239"
     CDS             20623..21198
                     /locus_tag="WJK66_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ7578011.1"
                     /translation="MVGGTDAESDESLLARYEERLRRPAAGGNQYDFRNWCLEVPGVV
                     DAFIYPLRRGNGFVDAVILGENGIPSAETLAAVQAHVDAVRPVTRKNGFLALAPSIQT
                     VNVAVTITLSSGTDTDTATAAIKSAVNAYFDALKPGDPLIKSQLETLISEVYGVRDRV
                     LTTPVGNIKPQESAEDIYWLRPGSINVEYTT"
     gene            21195..21761
                     /locus_tag="WJK66_000240"
     CDS             21195..21761
                     /locus_tag="WJK66_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HEZ7578012.1"
                     /translation="MIHQTLLAAMRPPVSYDTVGETAEIKAEAGVFDIVADHAEGVKN
                     APFPDAENDYLYRWEELLAITPPAGANTQQRTDAVLAKLNALGGLSIAYFTAIAESAG
                     YTVTIYEEDQFRAGESCAGDCLNTEDAIWRWCVDIADGKATAYIFRAGQSRAGDRISV
                     YTDPIIETMFEELKPAWTYCRFEYEEEV"
     gene            21764..22999
                     /locus_tag="WJK66_000241"
     CDS             21764..22999
                     /locus_tag="WJK66_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578013.1"
                     /translation="MDLIQTPNKQFVDGDRRTPGTPVPAWWLNQLQGELYSILNAVGI
                     EPNKADHAQVLSAIKTLAADASQVASIDALRKYSGTGYVNVNAYHANTTVGGGVFVAD
                     KADKSTADNGCTVIVSTDGTRWKRVFSGMLNLHDFGYVASKNNALSTLNAAESAALDV
                     VVDCLGLSIDTGNTYPQKNKYTNGKFVINGKTVDVQYQPIRSGIGRFISGTGAAANLK
                     SNEWTGAGLIVIGEGAMEQMEKCVSSIAIGDRAQGFSKVSRDNIAIGADSLINVQAAT
                     EWYDQSRMEGTRNIGIGGNAGRGITSGYSNVSIGRNAGQGLGEGSSNIALGAGAMAGT
                     APVGFSGDIEVFWPSSTSRTIAIGEAVLQTYQGRAAQTAIGANAARNTKKAEKVTAIG
                     SAAMENLERNRAPNGGDVV"
     gene            23162..24046
                     /locus_tag="WJK66_000242"
     CDS             23162..24046
                     /locus_tag="WJK66_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEZ7578014.1"
                     /translation="MNGNTLIIQSSKELTATGAAELKYVYSVNSTATKNEELTIIGAN
                     AMNKALTAGYSTIIGADAALLGDNYQKTTAIGASSLRTGSHISTTAIGYWVIPLASSE
                     KCVAIGDSAGYRNVQGDFLTGKITNSIAIGYGARINGDNEIQIGTTGQTLYAPTAVNI
                     RSDGRDKADVKPLTNGLDFVMKLKPMTGYYDRRDSYVDELFKDLPADERADKVREWWA
                     NPIKDGSHKEDRLRHWFIAQDIAALEDEYGRLPMVNKTNDTYTVEYETFIPVLTKAIQ
                     EMAARIETLETEMKESKK"
     gene            24043..24666
                     /locus_tag="WJK66_000243"
     CDS             24043..24666
                     /locus_tag="WJK66_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578015.1"
                     /translation="MTRCVIDQDGLFVEEQYFDDGRQSIEAEMPDLAQYQAAQWDGQG
                     WKIIPDYRGCVVFAGGQEQVWDKLGDLPDGVSLTPPESVNIDGLKSVKLVALNAAAQA
                     FINKHAGIDSVPEFEFASWAIQAAEAKAWQEDKAAPTPVLDGIATARGMSADTLKAAA
                     LRKTLAYEQLAAHVAGQRQALQSKIEAAKTQAALDKIAVVFTLPEAV"
     gene            24667..25155
                     /locus_tag="WJK66_000244"
     CDS             24667..25155
                     /locus_tag="WJK66_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578016.1"
                     /translation="MVQVYLALYKGKAAINTPRDVVKRIADSVVRLATCSPYSHCEIA
                     VKHPRDGLFDCYSSSARDGGVRIKTMPLPADKWDLIPLPQSVAISASRLFHRTHGAGY
                     DWLGAIGVVLKSPHSKSRWFCSEWCAYVIGYTNPCRYSPQTLYAAVSTKDRPSEKMEE
                     TK"
     gene            25503..26342
                     /locus_tag="WJK66_000245"
     CDS             25503..26342
                     /locus_tag="WJK66_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEZ7578017.1"
                     /translation="MMQKYHSTAPLPFVGQKRYFIKHFTKVLSQIPADGKHWTIVDVF
                     GGSGLLAHVAKRIKPQARVIYNDYDNYSDRLRHIPDYNRLREQIAQIVGGIPKGSRLD
                     PERTRSVQQTITNFQGHIDVRVLSSWLLFSAKQANSLEQLLGFEFYNKVRQSPYSIAA
                     DYLDGLEITQQDYNLLMAEHQHNPNTLLVLDPPYVSTAQGAYAADKYFNMVSFLRMIQ
                     YMRPPFILFSSTRSEALDYFQFLQECEPDKYRRFSGYNIVSLDAKMGKGIEYQDNMIY
                     KID"
     gene            26602..27135
                     /locus_tag="WJK66_000246"
     CDS             26602..27135
                     /locus_tag="WJK66_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEZ7578018.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(27198..28382)
                     /locus_tag="WJK66_000247"
     CDS             complement(27198..28382)
                     /locus_tag="WJK66_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEZ7578019.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTSATSVPPLPSFHSVVK
                     ARTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNAGFTVTNQQAGSRRF
                     QRFAGTDSQRAADFQDVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGT
                     LFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVD
                     VPYIQRADVETEGILWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNT
                     LYLSGILKKQRAIVFGNFRMEKIRDVYDPSYDFSAVANHVSRTAKIPVLTGFPFGHIA
                     DKITFPLGAHARIRMNGNSGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADI
                     SE"
     gene            complement(28422..28826)
                     /locus_tag="WJK66_000248"
     CDS             complement(28422..28826)
                     /locus_tag="WJK66_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEZ7578020.1"
                     /translation="MTAQPCPICTAQDEDILLQTPKLRVIAVHNDSGSPAFCRVIWRK
                     HIAEMTDLSAAERGELMEMVYKVEAAMRQVFQPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
BASE COUNT         7371 a         7524 c         8087 g         5889 t
ORIGIN      
        1 caaaaggcca acgagcaaga gccgttcatc gcggcggtgg ccgaaacttt aagccgcacg
       61 atgtggggct atgtgatgcg gatggatgcg cggtttgaag taacgtttat cgtagatttg
      121 ggccatgtgg ccttcgagca tcaattcact gcggtcaaac aggcgctgaa ggaaaaattc
      181 gaggttgaag ccgatgggga tgcgctgact gtggaatccg accgcctgcc ctatggtatt
      241 gcggcctgcc gggtggtgtt cggagatgtg ttatgagcgg agatacaccg attaccgtgg
      301 aatatgtgtt cggcacgttg gtgtcgtttc tgattgcttt attgtggtat tgggtaaaaa
      361 gcatttcaga cggcctgaaa gaagcccgca aagatcgtga tgaactgctc ggcaggttgc
      421 acagcgtaga aacatcttat cagacgaaag cggaagcaaa agacaacagg aacgaaatct
      481 taaacttgtt gcgtgaaatc aaggctgatt tgaaagaagt cggccagaaa atcgaacgga
      541 aggcagacaa ataatgcaaa accaagaccc tattttaaga gcgttggcag aaatcaacgg
      601 caagcaggac aaattgttac aaaatcagga acgcatggat gcggaaatca agaaaatcca
      661 tgccgattgc cgccgcacgt cggcaaccac cggcgcggcc gcgggcgcga tttcgggcgg
      721 tattgtggct acgggcatcg catttgcccg cgccaagttg gggctgtaac gatggcgcat
      781 ccgaaggcga cccgcgacaa gctgcgggca ctctactgca acggcgagca gagccttgag
      841 acggcggcgg ctttatgcgg cgtatcgctc ggcaccgccc gccgctggcg cgatgaggcc
      901 aaggcgcagg gcgacgactg gaacaagctg cgcgcggccc atacgctggc cggcggcagc
      961 atagacgaaa tcgcgcgggc gatgatgacg tcgtttctgg tgcagtatca ggcgacgatg
     1021 acgatgttgc aggatgcgga agtggaagat ttgccgccga gcaaacgggt gcagctgctg
     1081 gcgagtttgg ccgatgcgtt taccaaaacg gtggcggcga acaagcgggt gttgccggaa
     1141 acctcgcaac tggcgacggc tttggagttg ttgcagtttt tgatggtatt cgtgcaagaa
     1201 aaacacccca aacatttggc tgcctttgtg gaagtgctgg agccgttcgg ggcggaggtg
     1261 gagaggaagt ttggttagtt taatcgtgaa gtaaacgtgt catcagaatg tttaccggca
     1321 acaccaattg caaccaatag atctagatga tcggctaatt ttttcagtga aattttaaag
     1381 ccttgctcct ccaataaatc ttgtatttca gacggcgtgt atttgcccgc atctaacagt
     1441 ttggatattt ctaaatcaat cgatttcatt ttttgccctt atgaaaaata aagatttcct
     1501 caaatccctg tccgccctcg ccgccaatct gcgccaagtc atcgaggccg aagtagacgg
     1561 tttcgatgct tcgccgacgg cggtggccga gcggcgggcg aaggtatttg acccggtagg
     1621 cggttacgag tatttcgtca acacctactt cccgcattat atccgttcgc ctgaaaaatc
     1681 cgacctgcac cgcttcttat tcaggcgttt accggaaatc gtcgaatccc cggaaggcga
     1741 gaacgaagcg gttggcgcgc cgcgcggcga gggtaagtcg acgcaggtga cgcagttgtt
     1801 tactttgtgg tgcattgtaa cgggacgcaa gcattactgc gtgattgtga tggacagcat
     1861 agaccaggca tacccgatgc ttgaggccat caaagcggaa ttggagttta atccccgcct
     1921 gaaaaccgat tttgcggatg tctgcggcca aggccgcgtg tggcaggcag gtaccatcgt
     1981 aacggccaac gacatcaagg tgcaggtagc gggcagcggc aaaaagctgc gcggattgcg
     2041 ccacggccct taccgccccg acctgacgat tttggacgat attgaaaacg acgaacaagt
     2101 ccgaaatccc gaacagcgcg acaagctcaa tgcgtggctg acgaaaaccg tgctgccgct
     2161 cggtggtgtg gggcaaaaat acgatgtgat ttatatcggc acgattttgc attacgacag
     2221 tgtgttgaac cgaacgctca ataatccgtt ttggaaaggc atcaagttta aagccatgtt
     2281 gaaatggccc gaccgcatgg atttgtggga cagatgggaa gagttgtacc gcaatgaagg
     2341 tgagttggtg gccgatgcgt tctatcaaac gaacaaagcg gaaatggaac gcggggcgga
     2401 aacttcttgg gcagcgcgcg gcgtgctggc tttgatgaaa atccgcgccc gcgacggaca
     2461 cgcggctttc gattcggaat atcagaatga tccggtgagc ggtgaagatg caccgtttgc
     2521 ccaagccatg cagttttggg cggaactgcc cgccgatttg gtgtatttcg gcgcgctcga
     2581 cccttcgctc ggcaaagcgg gggccagccg cgacccgagt gcgattatcg tgggcgggta
     2641 tcaaagaagc agcggcaagc tgtttatcgt ggaagcgcaa attaagaagc gtttgcccga
     2701 tttgattatt gaagacgtta tccgcttgca tgcgcaatat aagtgcaagc tgtggtttgt
     2761 ggaaacggtg cagtttcagg agttcctgaa agacgagctg gtaaaacgaa gtgcggcaaa
     2821 aggcattccc gtgccggcgc gggcggtcaa accgattgcc gacaagctgc tgcggattga
     2881 gactttgcag ccgcacatgg ccaacgggtt gattttactt catgccaatc agcaaacctt
     2941 aatccaacag tttcgccatt ttcccaaggc agaccatgac gacggccccg atgcggtgca
     3001 tatgttgtgg tcgggggcaa cggccaacag tgcgccggtg gaatatatgg cggtgacgaa
     3061 acatgggacg gacggcggtt tcggcagcgg cgcatggtga aactgacacc gggcagggct
     3121 tacgattaat gcaggcagca gagaattggg gcaagtttaa cttgcccttt ttttatttat
     3181 gaaaaacatt ttcagcagtg cattgagcaa aattctgccg ggccgctata caccgaaatc
     3241 taccccgcaa acggcggaaa ttacccaaaa cacccagact cacgaacacc cgagcaaagg
     3301 tttgacaccg cagaagctgc acggcatttt ggaagctgcc gagcgcggcg atatgaaggc
     3361 gcagtcggaa ctctttgccg acatcgagga gaaagacggc catatctttt ccgagatgag
     3421 caagcgcaag cgggcggtca tcgggctgga ttggcgcgtg atgccgccgc cgaacagcac
     3481 cgacgccgaa cggcggctgg ccgaagaagt caagggatgg attgagcgtc tgcccgattt
     3541 cgaagacatg atgttcgacc ttttagatgc ggtcgggcac ggctttgcct gtgtggaaat
     3601 cgaatggcag caaataggcg gcctgtggct gccgaaaaac tttatccacc gtccgcaagg
     3661 ctggtttaag gtggacggtg ccgataatgt gcggttggcc aaacaggata atccggatgg
     3721 ggaagagctg tgggcgttcg gctggctggt acacaaacac cgcagccgtt cgggtttgct
     3781 ggtacgcggc gggctgatgc gcacgctggt ttggccgtat ctgtttaaga attattcggt
     3841 gcgcgatttg gccgagtttc tggaaatcta cggcctgccg acgcgtttgg gcaaatatgc
     3901 ggtgggggcg gacgaaaccg ataaaaccac gctgctgcag gcggtaaagg aaatcggaca
     3961 caacgccgcg ggcattatcc ccgaaaccat gaatatcgaa ttgcttaatg ccgccaacgg
     4021 cagcagcgag ccgtttatgg cgatgatcga ctgggcggat aaaacatcgt cgaaagcgat
     4081 tctgggcggt acgcttacca gcatggccga cggtaaaacc agtaccaacg cgctgggtca
     4141 ggtgcataac gaggtgcgcc atgatttgtt ggtgtcggat gccaagcagc ttgccggtac
     4201 gataacgcag caactgattc tgcccctgct gcggctgaat aaaggcaacg tggatgaaac
     4261 ctgcctgccg cgtttccagt ttgatacgca gctacccgaa gatatggcgg tttacgccga
     4321 atctttgcct aggctggtag agatgggcat gaagattccg ctggcgtggg cgcaggaaaa
     4381 actggcgatt ccgctggctt cggacgacga gccggtattg gctttgcaga cggctgaaag
     4441 cgaaggtgtc aaagttgcgc cgttaagtta ccgccgcgtg gccttgagca ggcagggcga
     4501 aatcttggat atggggcagg cggccatcga taacgcaggt ttgggcaaaa tcgccctgcc
     4561 cgagcatatt gagccgtttt tgcgcgggtt gggtcaggct ttggccgagg gcgacagcta
     4621 tgaagaggtg caggagcggc tgttgcgtgc ttatccgcat ttagacagcg ccgaatttca
     4681 gacggccttg gcgcgggtga ttttcatctc cgacctgtgg gggcggctca atggctgatt
     4741 tgggttttgc cttcggtttg gagccggaag cggcggtcaa atattttgaa ggtttgggct
     4801 accatatccc gcccgactgg gatgtgaaat ggaatgaggc gcagaccaag gcgcggacga
     4861 ttgcaggcat acacaggcag gatattgtcg gcgagtttca cgcggcaatg tatgaggcgg
     4921 cgaaatcggg caaatcgttt gaggcttggc gcgatgaagt gcaaggccgt ctgaaagcgc
     4981 atgactggca tctgctcaaa gacggcgata ttgtggacgg agacaccggc gaagtcctcg
     5041 gacgcggtat taccaaacac cgcatggaaa cgattttccg cactcagatg caatcggctt
     5101 acatggccgg tcattggcag gcgtttgaag aaggtcggga tgatgcgccg tggctgcaat
     5161 attcggccat tttggacagc cgcacccgcc aaagccatgc ggcggcgcat ggtgcggtgt
     5221 atcacatcga cgacccgttt tgggattact tctacccgcc caacggcttc aattgccgct
     5281 gtaccgtgcg ggcgctttca gacagtgatt tgaaacggcg caatctgctg ccgcaaaaag
     5341 cgcagcttga agatacggaa gtggtggtca accgcaaggg cgatacccgc cccgccaaag
     5401 cggtaaagct gccggacggc cgccgctttt ataccgatgc gggctttcag cacaatgtcg
     5461 gtaaaagcca tttggccaac ttggggcagt tgcagatgca gcgtgcagtg gaactgccgc
     5521 cgaaactggc gagcgtatcg attcagacgg ccttaaaaca gcctgatttg atgcgggcgg
     5581 tatcgcaaca ggcggcgcaa atggtacggc gggtggatgc ggaaaaagta gcgcgcggga
     5641 aaacgctgta tgtgggcgca ttggccctgc ccgtgttgga cgcattggcg gcaaggaaaa
     5701 tttacccgca atccgccgtg attgccataa gcgacgagcg ggtattgcac gctttgcgcg
     5761 acaagaaggt caagccgctg ccggtatcgt tttgggagaa gatacccgaa ctactgcaag
     5821 agccggagca gattttattg ggcaaggcgg gacgaaatga cgatgccaaa cagtttttgg
     5881 catttgtgta tccgctgtcg gcaggcaagg ggaagctggt ggtaacgctg gactatgatg
     5941 tgaaaacccg acatccgttg acaggtaaga aagaacatct gaccttgaat atggtcaaca
     6001 ccggcatgat tgcagaaacg gataaacagg tggatagctt actgtacggg tatgaaacaa
     6061 tatggaaaaa gccataaaac tctttgcctg attcgaacag gataatacgc gacacggtaa
     6121 cgtggccgta acctttccag taggaaaccc gagttttatg gctgttggaa ccaatatacc
     6181 atgattgaga taaaaatcaa cacagacacc ctgcaaaaca gcttaaacgc tgcggcgcgg
     6241 catacctccc acaccaagcc tttgatgacg cggcttgccc gcatcatgcg caacgccgta
     6301 ctggagaatt tcgccgccgg aggccgtccc gcttgggcgc cgcgcaaata tccgactgcg
     6361 cgtgaaggtt cggggctgtt gcaagccagc ggccgcctgc gcaattcgat tacgccgagc
     6421 agtaccaacg atacggcggt ggtcggcacc aatgtggaat atgcggctat ccataacttc
     6481 ggtgggaaaa cctcgccaca cttgattaga ccgaaaaaag gcaaagcgtt gaaattcggc
     6541 ggccgctttg ccaagcaggt taaccacccc ggcagcaata ttcccgcacg cccgtttatg
     6601 accctgcaac cggaggacga aaaggcccta tccgatgcgg tggcggaata tctggcacag
     6661 gccattcagg ggcggtaatc atgcctgcct gaaaaatgcc gaaaaacagc ccgaaaacgc
     6721 cctaacccat acttacccct acccatcgcc tgaaaatcaa tcctgcgcgc gtttgaacac
     6781 cttttgaaca ctatcccacg cgtatctctg caagtacatt tcccttccct agaaattcat
     6841 ctgtctgaca taggccaagc tttgacgagg gcgcggctgc cgcacaatgc gggctatgga
     6901 caccaaaacc cttcttgccg ccttatccgc cgcccatgtc ggcggttcgg acggcttaat
     6961 caaaatcgta cccaaaggcc aattcgcacc ggttgacggc cgcaccgata cgggtgtgcc
     7021 gcactggacg atgtctgccg atttggcaca gcaaatcatt gccgcctttg acgccgcgca
     7081 aaccgacctt gtggtcgact acgaacacgc cacgctgaaa gccgccgaaa cggggcagca
     7141 aaaccctgcc gccggctgga tcagcaaata tgtatgggat gacgagcgcg gcctgatggg
     7201 cgaagtgaaa tggacgcaac gcgccaaaga catgatagac agcggcgaat accgctatct
     7261 gtcgccggta ctcgaatacg acacgctggg caatgtacgc gggctgcaca gtgtggcgtt
     7321 gaccaattcg cccgcactgg acggcatggc tctggctgca ttgagccgcc aaaactctat
     7381 caaccccaaa caggaaacaa gtatgaacaa ggaagcttta atcaagctct tgggcttggc
     7441 ggcggatgcc gacgacaaag ccatcgaagc ggctttggcc gaagcacagg aaaagctggg
     7501 cggtaaaacg cttgccgaag cactggccgc acccaaagaa gaaccgcaag gtagcgaagg
     7561 cgataaaggc gatgccggca aacccgaaga caagccgcaa ggcggcaatg ccgacgacgg
     7621 cgaggtagcc gaactcaaag cgcaagtggc cgcgttgagc aaaaaagtga ttgcaatgga
     7681 agtgggcggt acttcagacg gcctgatccg tgccgcgctt tcagacggcc gcctgttgcc
     7741 gcatcaagaa gcatcggcgc gccaactggc cgccaaagac cctgaagcgt ttaaagccct
     7801 gattgacggc agcttggcac tggccgcttt gagcaaaacg cagaccggcg gcaaaggcgg
     7861 cgcagacggt acgtccgcgc tgaccgccga agaagcagcc gtcgccgcgc aattgggcat
     7921 ctctgccgaa gattatgcga aggccaagta atccgataaa ggaaaagaca acatgattat
     7981 cacaccagac accttaaaag ccctgttcac cggctttaaa aagaatttcc aagacggcct
     8041 gaaaatggcg gaaagccaat acaaggaaat cgccaccgtc attccgtctt ccactgcgtc
     8101 caatacttac ggctggctcg gccaatggcc cgctttccgc gaatgggtgg gcgaccgcgt
     8161 attccaagat atgaaggcgc acggctatgc catcaccaac aagcattttg aaagctcggt
     8221 caaggtcaac cgcaacgaca tcgaagacga caacgtcggc atttacgcgc cgatgatgac
     8281 cgagatgggc cgtgcttcgg cggtgcatcc cgacgaattg gtgtttgcct tgctgaaaaa
     8341 cgcacacgcc acgttgtgtt acgacggtca gaacttcttt gataccgacc atcccgtgta
     8401 tgaaaaagtg gacggcaccg gtcaagccag taccgtatcc aatattttcg ccggcagcga
     8461 agcggcctgg tatctgctgg acactacccg cgccctgaaa ccgttgattt accaagagcg
     8521 taaagcgaag cagtttaccg ccatgaccgc cgataccgac gaaggcgtat tcatgcgcaa
     8581 cgaataccgc tacggcgtgg acggccgttg caacgtgggc ttgggcttct ggcagatggc
     8641 ggcgaaatcg caagagaaac tggacgctgc cggtttcgag aaagcctaca acgcgatggt
     8701 gagcctgaaa ggcgacggcg gcagaccgct ggccatccgc ccgaatgtgc tgctggtacc
     8761 gcctgctttg gaaaatgcgg ccaaagagct ggtggaaggc gaccgcctgg ccaacggcgc
     8821 gtacaacccg aacaaaggca aggcgaaagt catcgtatcg ccttggttgc tgtaacgaac
     8881 aggcgggcac cgcccgccgg aagggataga aaatggcaaa agaaaaaaac ggacaggaag
     8941 ttggcgcgac cgtcgatgtc caaccggaag aggtaaccgt aaccgctgcc ttacaggccg
     9001 agattgaagc cttgagagcc gaattagaca aagccaatgc cgaaattcag gtagcgcagg
     9061 cagagttggc cgccgcgcaa gagcgcaatg ccaaattgga aagactgctt gaagccggcc
     9121 ttgcacccgg cggcatttca gacggcgaaa ctttgacacc tgaagcggaa gccgcctttt
     9181 acaccggcag cggagcaccc gccgcagatg ccgaagtggt ggcgattaag agcaaacacg
     9241 gccatgcgtt tttccgcgcc ggctaccatg tacagccgaa ctggacgttt gtgcgccgtg
     9301 ccgacttcga acccgccgac tttgagcgcc tgattggcga ccgtatggtt gaagcgcgcg
     9361 aagccctgcc gacggacgca tcatgagcta cgccgccgtt gccgatttgg tggcgcgttt
     9421 cggcgaagcc accatcaccg gcctgaccga cttgtcccgc aagggcgcgg tggacgaaac
     9481 cgtcgcacag caggctttgg atgatgccgc cgccgaaatc gacggctatc tgatgaaccg
     9541 ctataccctg cctctgccca agccgctgcg catcttaacc gtgtattgct gcgatattgc
     9601 ggtgtaccgc ctgtgtaccg gcaaacggca gcttaccgaa gacattgtgc accgctatga
     9661 agcggcagtg aaattcctgc aactggtggc ggcggggaaa gtcggcttgg gcgttaccga
     9721 acccgcaggc gaaaagcccg ccgtacaagg caacggcgtg atgtttacca cacaggaaaa
     9781 ggtgttcggt cgtgatagcg tctattgaac aagccatcag gcagcggctt tcagacggcc
     9841 tcggccaaat ggtaacgggt gtctttactt acggcggcga gttcgacggc gaaggtttgg
     9901 cgcaggtggt caaccagttc cccgccgtgt gggtcatgtt tgccggcatc aaagacacca
     9961 tccgccacga tacgcgcggc agccgtttta aagcgattgg ccaattcacg gtattggtgg
    10021 gagaccgtgc cagcggcagc gaagcagaca gccgtttcgg cggcctgcac cgccatgatg
    10081 tcggcaccta ccgcctgatg cagacctgcc gccacctgct gaccaatcaa tcgctgggct
    10141 tacagataga ccgtctgcaa ccgggggcgg ccaaaagcct gttcagccgc caaatggagc
    10201 aagacgccgt tagtgtgttt gcgctggaat ttgaaacgta ttggtttgaa gacgcgctgc
    10261 aagacggcga ttggccgcgg cctgcggttt cagacggcca acaggcgaaa gtatatgccg
    10321 acgtatccga ataccaaggc cgcaccgaac ccgaacatcc cgactttaaa ggcgtgaacc
    10381 ttgaattgcg tatcccgccg aaaacccccg accaacccgc cgatatggcg gccaccgttg
    10441 aaaccaaggt gaaaccatga acgaaaccat taaagtacgc gccgccgacg gcctgcaagt
    10501 gccgctggcc ggtaaaccac atgaatacat taccgaccaa gaaaccgtta ccgtacccga
    10561 tgccgcctat taccgccgct gtatcgaata cggcgacctt gtggtcgtaa ccgaagacac
    10621 ccaacccgcg aaaggcagca aataatggca tccgcaaaca tcaatttcga aaaaatcccc
    10681 gccagcaccc gcaagccggg cgtgtacgcc gagtggaaca ccaagctggc cgtgcgcaac
    10741 ctgcccacca acaaacagcg cgtgttgatt gtggcgcagc acaacaatcc cgctttgggt
    10801 gagcttaccg agctggaaaa cgtgttttcc gccgccgatg cggccgccaa atacggcgca
    10861 ggcagcatgg cgcacctaat ggttaccgcc gcgattaaag cctatgccta tgccgattta
    10921 agcctgatta ccgtagccga caacaaggcc ggagtggccg ccggcggtaa aatcacctta
    10981 agcggtactg ccaataccca aggcgtgttg cgtgtgagca ttgccaatgc tgacacttta
    11041 accatcggca tcggcgccga agacaccgca gccaccgttg ccgccgccgt caaagccgcc
    11101 atcgatgccg tgcccgattt gccggttacc gccaccgttg ccgaagcagt ggtgacgctt
    11161 accgccaaaa acaaaggtac ggccggcaac gccatccgca tcaaaaccag caataccgcc
    11221 gaaggcatta ccgccgccgt taccgccatg acgggcggcg atgccaaccc cgatattgcc
    11281 gccgcgctca atgccgtagt cgccgaaggc caccacatca tcgcctgcgg tatcaacgac
    11341 gaaaccaacc tgctgaaact gcgtgcccat ttggataccg tagccagccc gatggaaaaa
    11401 cgctgggcaa tatgcgtata cggccaaacc ggtacgctgg cgcaagccac caccttggca
    11461 ggccgtctga accacggcca tatcgtcagc gcgtggtatc gcggcacccc cagcctgcct
    11521 tgcgagctgg ccgccgcctt tgccgctgtg atggcgagcg aagaagaccc cgcccgcccg
    11581 ttgaacacgc tggcattaaa cagcatcggc gtgtgcgaaa gcaaagacaa aaccatgcgc
    11641 accgagcagg aaaacgccct ttataacggt gttaccccga ttgaaaccag tccggccggt
    11701 acgcaggcgc aaatcgtgcg cgccatcacc acctacacca aaaccgccaa cggcacggca
    11761 gacgaaagcc tgctggacgt aaccaccgtg cgcaccctga tttatgtaag ccgcgcctgc
    11821 gtcgaccgca tcgccctaag attcccgcgc gacaaattga gcgaccgcac cccgccgcgc
    11881 gtgcgcagcg aactgattga cgtattgatg tgctgcgaag agctggaaat tttggagcgc
    11941 gtggaagaaa acctgccgaa actgattgtg gaacgcgacc tgcaaaacac cggcatgttg
    12001 aactgccgca ttcccagcga tgtcgtcaac ggcctgcatg tagtaggtat ggttgtagat
    12061 ttatatttgt aagaaaggct gaaacatgag taccgaatac gttggcagcg tcatcctcta
    12121 tctgggcgcg aacgaagtcg aagtaaccaa aatcgacgtg aaagacaaca ccggcaaaaa
    12181 gcccgtaaaa accatgaacc gcacccgccg cgtcaaaggc tttacccgcg gcgtcggcca
    12241 atacgacatt acctttaccg ccgtggtgcc gaccgacggc acggcggtgg actgggcaaa
    12301 gatcgacgac gccaaaatca gcctagttcc cgacgtggaa ggcgcgcgcc cgacatcgta
    12361 cctcggcttc tgcgccaccg aagcgggtga cagctatacc gtagataatg agctggtggt
    12421 ggatgtgacc ggttttgcga ttcggaaggt gttggagtaa agattttctt gttaacagaa
    12481 gccgctgaag cggcttctgt tgtttttatt acactgtttt taataaaatt caatttttca
    12541 agtaaaatac ttgaatttta aaaaagcctt tgatattatt tagccacgtt gaatgtggca
    12601 gtaatgctgc aaacacatga aaaggagctt gaacaatggg actgactaaa ttcggcgcag
    12661 ccgttcgtga ggctcgtcgt caaactaaac aaactctgca aacgatggct gctacattag
    12721 gtacttctcc tgcttttttg agtgctattg aaactggtcg tagtaaagtg ccaatggatt
    12781 ttgttaaaaa ggttgaagat ttttttgaaa acttaggcca accaatagac ggtttaaagc
    12841 aaaaagcgat ggtttctaat gagaatgtat cgttaagcgg cttaagcctg caacaacaaa
    12901 tgctggtggc tggttttgcc agttcagact tcagcaaaga gcaattggac aaatttgctg
    12961 aattattaag aaccattcat cataaagatt cacagaaaga aggggatgat gcagcaaact
    13021 aattattcaa tgcgtggtgt gcgtgtggga atgttaagcg aagaaacgat tcagtttttt
    13081 gcaaacgaag ctgccaaatt tttggtagta gataagcgta ctcgtaaaaa aatggatgca
    13141 tttatggaaa tgctggaaga gtatggcatt gtaattgatg ttgttgctga ttctgaatgg
    13201 ctagtcatta ccaacgcaat gtgccataac ggaacgattc tgatgcctaa tagcctctat
    13261 actcgcattt gcaatggcga ggacgaggct atctttattt tctttcatga attggggcat
    13321 ttgcttttag ggcataaagc catgttgcat tatagtgaag ttcttcccac gaaacaggaa
    13381 gattcagaat ggcaagctga tgagtttgca aaatgtattt taagaaacat gggaattaaa
    13441 tatgtaccag aacagttaag cttacgattt taaaaagaga aatgacttgg aatgttggcg
    13501 catcccaagc cattattgtg gtgtactagc atacttccaa tatgctgtac aagtgtagtt
    13561 ctccatccgc taactgcaaa cagatttcaa agagaaatac ttgaaaggga cttgagtata
    13621 tatttttcag atttgtttgt ccacgaaaag cgaagtgatg gccaccattt ttacaattgg
    13681 aggtgccatt atggctatga ctaaggctcc cgttaccccc gacttggtgg acgatacagg
    13741 tgcatacatt tttagaaagt cttttaccac caaaagtggt aagaaaattg tttctaaaaa
    13801 tgggaagcca tttaaaattc ctgtgaaagt ttaggaatat ataagccccg ctaagcgggg
    13861 ttttttcatt actgacgcgt gtttagtcgc ccatctcatc tccttaagtg acaattaagc
    13921 cttgatttat acaaggttta accaacgttt aagggctttt ttatgtctga attttccccc
    13981 gaactcaccc gcgctatcga agattacgaa ttacgcgttt ctcccgacct gaaaaccgtt
    14041 gccggccgtc tgaaatacgg catcggtgtg attgatggcg aaatgcacca tgatttttcc
    14101 atgcaccttt taaccgtgcg cgaggacatg gcgattgatc cgcaacttga aggccagccg
    14161 cggctggttg ccgcctatgc cgcgtcactg gataaattgg gtggtttaac agccgaatct
    14221 ttgacacctg atttgctgct tgatgagatg gctgctgctg actttgacgc gctgtactgg
    14281 gcgcaagagc tgctgcaaaa aaagcggctt tgcccgcacc ccgcgccgat cgttaccgat
    14341 acgccgtctt aaaactcggc cgatacggca tcacgcccgc ccaaatcgac ggcatgaccc
    14401 aacccgaact ggaaggctgg ttaaaacagg ccgacttaat cgagcgcggc cgtgccgcgc
    14461 cggtagtgat gccgtggttt attcctgcga acgtcaaacc atctgccgcc acgggcggtc
    14521 agacccaaac ctttatcagc aagcgtaaga aaaaatgagc cagcatagtg tagaactggt
    14581 tgctaagttt aaggacaacg ccacgcccgg cctccgccgt ttggcggcgg aagcggaaaa
    14641 aacaggcagc cggcagttgc agaaagccgc agcagtcaag ctgaaacatc aagagatgta
    14701 cagcgctacc gcccgtttgg gcatccgtac tgaacaccaa atccgccgtg aaatccaaca
    14761 aacgcaggcg gcttacaacc gtttggccaa aagcggcatg gcttcgcaac gcgaactggc
    14821 gagggctgca cagataacgc gcagccgtgt acgcgagctg actgccgaaa tcaacggcgg
    14881 tgcaagccgt ttgcaacgta tcggttcggg agtccggaca atcgggcgcg gtgtggccgg
    14941 cgtggcggcg ggtgcggcgg caggagctta tgtattggcg cagccggtca accggacgat
    15001 ggactttgac acatcattgc gtcatgccgc caataccatg tatgcaggca agagcatggc
    15061 cgagaagcgc gcaggtatgg aggaaatcaa gaaatcggtc atggatgccg cctatgtcgg
    15121 cggaacgacg cgtgatgccg ctttggaagc catgaataca atggtggcca gcggagcgat
    15181 gagcgacgaa gccgtgaaaa aactgctgcc gaccgttatg aaaaccgcca ccgccgccaa
    15241 tacggaagga aacgatattg ccaacatcgt aaccaaggcg ttgcaggcgg ggtttaaaga
    15301 ggcggatatt ccgtcgctgc ttgaccgtgc gttgcaatcg ggcgcggacg gcggttttga
    15361 attgaaagac atgtcgcgct ggctgccgca gcagttggct gcaatgaaaa acgccggtat
    15421 gggcgcgacg ctggataatt tttccagcct gctcaatgcc aaccaattgt cgtttatgac
    15481 ggcgggcagt accgacgaag ccggcaacaa cttagtcaac ctgctggcaa aaatcaacag
    15541 tcaggatatt gtgaccaaag ccaaaaaaat taccatcaac ggcaaagaag gtttcgattt
    15601 caccgctagc atgaacaaac ggcaggcagc aggcatgaac tcgcttgatg cgctggtgga
    15661 cattgtcgga gaaatcacgg ccaaggataa gaagtccgcc gctctgatga aacagattaa
    15721 tgccgcgcaa ggcgatgaag ccaagctggc tttgctggaa aaccaaaagg ccttggtaga
    15781 cggcactgcc atcgggaagc tggtttccga ccgtcaggca ttgatggcat tgctggcatt
    15841 ggtcaacaac aaacaggaac tcacccgcct gcaagcaggc cagacaaatg cggcgggtgc
    15901 ggtggacggt gcatacggat tcgtcgccga aggttcgggg tttaaaaaat cccaattctc
    15961 cctcgccaaa tcagaagccg aatacggcgc atttgaaaaa ttcagcgacc gcatcggcgg
    16021 cggccggtac aagctctatg tttgggggcg gcggcctgct ttccaaactg ttcggcggaa
    16081 gcagcagaac cttacaggcg ggccgtctga tgccctcggc ctcctcgcta ggccccgtcg
    16141 cgctcggtgc gacaccgttg gccgtaatgg gcggggcaac ccatttggcg gggcagcgcg
    16201 acaaatatgc ggaatggtca aaaccgtttg ctaaattatc gtcttggctg gaaagtatcc
    16261 tgcctgattt ttcagcggtg gcgaaagccg aatatctgcg caaacgtgaa gaactgggcg
    16321 gcaataatgc cccgctcgat agtcctgttt taaaagagag tatggcgcag cttaaccaat
    16381 cggcacaaac caaccagcag gcaagccagc agtatgtgca ggcggcggcg gagaatcagg
    16441 cggcaacggc gcagattacc ggcgcggcgg cacagatgac ggctgctgcc gcacaaatgc
    16501 aggcggcggc aggcaagccg attccgatta ccgttactgt gcaaaacggc aacattatgg
    16561 cctatgtgaa tcaggcagta gaacgcaaca gtaggaaaaa ttaatggctt ggaaagacac
    16621 tttactggat gcgggcttca agggcgttac ctttgacgtc atcgacgaca cgctgcgcgg
    16681 cacccacgcg ctggcggaac acgaataccc gttcgtacag ggcgcggaca tcgaagatac
    16741 gggtgtatct gcgatggata tggccttgac cgccgtattg tggggtgaag attacgaagg
    16801 ccgcctgcaa aacctgctga acgtattgtg cgaaacgggc gcgggcgaat tgatccaccc
    16861 gatttacggc agcgtgccag attgcgtagt ggcggatttc gaggtcgcgc acaacgaaga
    16921 aaaccccgac tactgcaccg tgcggatgac ctttaagcaa agtgtcaaag ccgcgccgtt
    16981 tttcgaccgc gacttgccga tagccttggc cgacgaagtg gatttccttg ccgacttggc
    17041 cgcatggcag ggcttcgagg tgtttcagac ggccttaaac aaaattcaga aagcgcaaag
    17101 ccgctggaat gcttttcatg ccacggtatt aatggtggtg ggcgtattgt atgggcaggt
    17161 aaacggcatc ttcaccggcg gcctgaacct gctgaacagc ccgcgcgtat tggtggcgga
    17221 gctgaaatct gtgtttggtg cgctggccaa tatgcacaat gtcggtaaaa gcgggctgga
    17281 cggctggcgc gacatggtgg gcggcgtatc taaagcggcc gctacgccgt ggcaggtcag
    17341 ccgcggaacc gaaggcagtg tggcggcggt agatttaatc caacgcgcca agcccgaaga
    17401 tgtggctgcc tttaccgcgc ttaccgccac cgttggcgcg tgtgcgctgg ccgaacaagc
    17461 cgccgatatt ttggccgtac aactgaacga gccgactttg acgcctgtgg aaatctcgcg
    17521 tctgctggcc gatacccgtg ccgccttgca gcgtgctttg gccgcccagc gcatcttggc
    17581 catgatgctg gcggatgaaa ccaaagccga caagctggcc tattgcctgc tgcggctgta
    17641 tcaaacaccg gtagacagtg ccgatgatgt gtaccaacgc atcgaagtgg ccgggctgct
    17701 gccgcaagcc ccttatctgg aaactgccgc cgcgcttacc gaaagtctgc gcaatacggc
    17761 gcataaactg caaaaacagg cgtttacagt catcaatatg aagccgccgc tggtacagaa
    17821 aaccgtcacc tgcgatatcg gcctgcacct gctggccttt gagtggtacg gcgactacag
    17881 ccgctttggc gaactcttgc gcctgaatcc gcaaatccgc cacccgaatt ttattgagaa
    17941 aggcacggta ctgaatgcct acgccagata acaccgttac gctgctgatt aacggtaaaa
    18001 cacacggcca atggacgaat tacgacattg tttccgacct gctcaccccc gccgatgact
    18061 tttcagtcat gctcggccgc cccgtcgatg ccaaacccga tgcggtgcgg gcgggcgaca
    18121 aggtggaagt gcgtgtgggc ggcgatacgg tattgagcgg ccgcatagac cgcgtgcaga
    18181 ccgttaccga aaaaggcggc aaaaccttaa ccatacaagg ccgcgacgat gcgggcgtgc
    18241 tattggactg ctccgccccg ctgtttaacg cgcaagatat ggatttaaac caaatcatcg
    18301 aaaaaatcgt caagcctttg ggtttggcca aaatccgcat tgatgccgcc aaaaccgaca
    18361 aaacccacaa agtgcagatc gagccgggca gccgcgcgtg ggacgccctg ctggaatatg
    18421 ccgaggcaaa cggtttgtgg ccgtggctgg agccggacgg cacgctggta gtcggcggtc
    18481 ccgattacac cgccgcaccg gtggccgaac tggttttacg gaccaacggc caaaacaaca
    18541 acatcaagcg tctggaagta aaccgcgata tggcggcacg gtacagcgaa gttaccgtat
    18601 tggcgcaaag ccacagcggc aaaaacaaca tcaaagccac cgccaaagac gaatccgtca
    18661 aactgcaccg ccccctaatc gtcaccgagc cggacatcga cagtcaggct caagcgcagc
    18721 gcaaggcgaa aaagcggctg gccgacagca ggctggaagg cttaacaatt accgccaccg
    18781 tacaaggcca ccgcactgac gacggtacct tgtggcagcc cggtcagcgc atcaacgtat
    18841 tgagcgaacc ggacggcatc gacgcggtgt atttcctgat ggcgagaacc ttcatcggcg
    18901 gtcgcggcca acccaccgaa accgtgctga cactgaaaga ggacggcgca tgggtattgg
    18961 atgccgaccc gccgaagaaa tcgggcaaaa ccaaaaggcc gtctgaaagc cgcaaagcca
    19021 acggccacgc agccgccaaa ccgaaaaaac gccgccaagc caagcaggcc ggacaggaat
    19081 tgcaggtgat ttaaatggat gcaaaaacca tagacaaccg tatccgacgg gcatttaacg
    19141 gcatccgcca agccttccgc ggcaaaatcg cccgcgtcaa agcagccggc ggcgtgcagc
    19201 aaatccaagt ggaaggtttg gaaggcgaaa ccgtgcagga cttggaacac gccgaaaact
    19261 tcggttttac gtcgaacccg cccgcaggca gcgattgcgt cgtcgtaccg ttgggcggca
    19321 aaaccagcca cggcatcatc gtcaccacca ccaacggcgc ataccgcatt accggccttg
    19381 ccgaaggcga aacggcggtt tacaacgccg acggtgccaa gatggtgtta aagaaaggcc
    19441 ggattatcga aattgactgc gaaacgctga atatcaaagc accgggcggc gtgaatattg
    19501 acgcgcccaa tgtaggctgc accgcgcaga ttaccgccga gggtcaaatc aacggcaacg
    19561 gcggcatggc agtgaaaggc ggcagcggca catcatttac cggcaatgtg cagatggtcg
    19621 gcgatttgga cactaccggc aaactgacca acaacggcaa aaatgtcggc tccgaccaca
    19681 aacacaacgg cgacagcggc ggcacgacat ccgacccgct gtaattcaga cggcctgaca
    19741 tccatcaaac caaactccaa gcgtccttac ctgaaaataa aggtatggac gctttactta
    19801 atccccaaac gggcggctat gtggtcaacc aatccgccca atccatcgaa aacgagttgt
    19861 atatccgctt ggtcacgccc ttgggcagtt actgggcaga ccgcacgctc ggcagccgcc
    19921 tgcacgaatt gcgccgccaa aagcatttaa agcgcatcga agtactggcc aaacagtacg
    19981 ccgagcaagc cttgcagccc gtgattcagt ccaaacgcgc ccaatccatc caagtaaccg
    20041 catccgcccc gcagcacggc tggctgaagc tgcatattga agccgtcgat gccgcgggcg
    20101 atacagtaac cctgaaccac aaggtagccg tgatatgacg caggcactga atttagaaca
    20161 aatccgcgcc aactatctgc gcgacctgca aaaccaaaac cctgccgccc atgtacacgc
    20221 gggcagcgac aaccatgtac gcgccaccgc cattgccgca gtgggcgaag gccaatacca
    20281 gcatcaggag tggattttgc gccaagcctt tgccgatacc gcagacagcg cctacctaga
    20341 aaaacatgcc gctaaatacg gcatctaccg aaaaaccgcc accttcgcgg gcggcaaggt
    20401 gcgtgttcgc ggcgcggtcg gcgcaacggt gccggttggc caacagatca atgtgggcga
    20461 taaggtgtat ttaaccgccg aatccgccgt tatcagtgcg cttggaagtg ccgaaatagc
    20521 cgttatcgcc actgttgcgg gtagtgctca aaatcaaacc gccgaaaccg cggctacgct
    20581 gcaaagcgta cctgcgggga ttgacagttc cgccgtctaa cgatggtcgg cggtacagat
    20641 gccgaaagcg atgagagcct gttggcacga tatgaagaac gtctgcgccg acccgctgcg
    20701 ggcggtaatc aatacgactt ccgcaattgg tgcttggaag taccgggtgt ggttgatgcc
    20761 tttatctacc ctttgcgtcg cggcaacggc tttgtcgatg ccgtcatttt gggtgaaaac
    20821 ggtatcccca gcgcagaaac actggccgcc gtacaagctc atgtcgatgc ggtgcgacct
    20881 gttacccgta aaaacggctt tctagccctt gcgcccagta tccagaccgt gaatgtggcc
    20941 gtcaccatta ccttaagcag cggcacggat accgatacgg ccaccgctgc catcaaatca
    21001 gctgtaaacg cctattttga tgccttaaag cccggagacc ccctaattaa aagccagttg
    21061 gaaaccttaa tcagcgaagt gtacggcgtg cgtgaccgtg tcttaaccac ccctgtgggc
    21121 aatatcaagc ctcaggagag tgccgaagac atttactggc tgcgcccggg cagtattaac
    21181 gtggagtaca ccacgtgatc catcaaacct tactcgccgc catgcgtccg cccgtcagct
    21241 acgacaccgt aggagaaacg gcagaaatca aagccgaagc gggtgtgttt gatattgtgg
    21301 ccgaccatgc ggaaggagtg aaaaatgcgc cgttccctga tgcggaaaac gattacctgt
    21361 accgctggga agagctgctc gccatcaccc cgcccgcagg agccaatacc caacaacgta
    21421 ctgatgccgt gctggccaaa ctcaatgcct tgggcggttt gagtattgcc tactttaccg
    21481 ccatcgccga atcggcaggc tacaccgtca ccatttacga agaagaccaa ttccgcgcag
    21541 gtgaaagctg tgcgggtgat tgtttgaata ccgaagacgc catctggcgt tggtgcgtcg
    21601 acatcgccga cggcaaagcc accgcctata ttttccgagc cggtcaaagc cgtgcgggcg
    21661 accgcatcag tgtgtacacc gacccgatta tcgaaacgat gtttgaagaa ttaaaaccgg
    21721 catggacgta ttgccgcttt gaatatgaag aagaggtata aaaatggact taatccaaac
    21781 cccgaataag caatttgtcg acggcgaccg ccgcacgccc ggtactcccg tacccgcatg
    21841 gtggctgaac cagttacaag gcgagttgta cagcatttta aacgcggttg gcattgagcc
    21901 taacaaagcc gaccatgccc aagtcttatc ggccattaaa acgttggccg ccgatgcttc
    21961 gcaggttgcc agtatcgatg ctctgcgtaa atacagcggc acaggctatg tgaacgtcaa
    22021 cgcctatcac gccaatacaa cagtgggcgg cggcgtgttt gtggcggata aagccgataa
    22081 atctaccgct gataacggct gtaccgttat tgtttctacc gacggcacgc gctggaagcg
    22141 tgtgttttca gggatgctta acctgcatga ttttggatat gtggccagca aaaacaatgc
    22201 actatccact ttgaatgccg ctgaatctgc cgcgcttgac gtagttgttg attgcttggg
    22261 tttgtcaatt gatacgggta atacctaccc gcaaaaaaac aaatacacaa acggcaagtt
    22321 tgtgattaac ggcaaaactg tcgatgttca ataccagcct atcagaagcg gtatcggtcg
    22381 attcatctcc ggaactggtg cagcagccaa cctcaaatcg aatgaatgga ctggcgcggg
    22441 tttaatcgtt attggcgaag gcgcaatgga gcagatggag aaatgtgttt cctcaatcgc
    22501 tattggcgac cgtgcgcagg gcttttctaa agtaagcagg gacaacatcg ccattggggc
    22561 cgacagcctg attaatgtgc aggccgctac tgaatggtac gaccagtcac gcatggaagg
    22621 cacgcgcaac atcggtattg gtggtaatgc aggacgcggc atcaccagcg gttactctaa
    22681 tgtgtcaatc gggcgcaatg ccggacaggg attgggtgaa ggctcgtcaa atattgcact
    22741 tggcgcaggc gcgatggctg gtactgctcc agtcggtttt agtggcgaca ttgaagtttt
    22801 ttggccgtct tcgacctcaa gaacaatcgc aatcggcgag gctgtcttgc aaacatatca
    22861 gggccgcgcc gctcaaaccg caattggtgc caatgcggcg cgaaatacaa aaaaggccga
    22921 aaaagttacc gcaatcggtt ctgccgcgat ggagaatctt gagcgaaacc gcgccccaaa
    22981 tggcggagat gttgtctaga cgggaacgga agcaggtacc tacgcccaat ctggaaaaaa
    23041 catcacgctt acatttccca acattcgcgg tgcgcaagcg acttattggg tgggcatccg
    23101 ccttacatca ggcacggcgc aaaccttaca aaacgacgtc gtaccggctc aggtcgtatc
    23161 agtgaatggc aatacattaa tcatccaaag ctcaaaagag ctgaccgcca ccggcgcggc
    23221 cgaactgaaa tacgtttatt ctgtaaattc aaccgctact aaaaacgaag agttgaccat
    23281 catcggcgcg aacgccatga ataaggcatt gaccgcagga tactcaacta tcatcggcgc
    23341 agatgccgcg ttgttgggag acaattatca aaaaacaacc gcaatcggcg catcatcttt
    23401 acgaacaggt agtcatattt ccacaactgc tattgggtat tgggtaatcc ctttggcaag
    23461 tagtgagaaa tgtgttgcca ttggagatag tgcgggctat cggaacgttc aaggcgactt
    23521 tttgactggg aaaataacaa actccatcgc catcggatat ggcgcaagaa taaacggcga
    23581 taacgaaatc caaatcggta cgacagggca aactttatat gctccaaccg cggtgaacat
    23641 ccgttctgac ggccgcgaca aagcagatgt taagccgttg acgaacggtt tagattttgt
    23701 aatgaagctc aagccgatga ctggctacta cgaccgccgg gattcctacg ttgacgaatt
    23761 attcaaagac ttgccggcag atgaacgagc ggacaaagtc cgcgaatggt gggcgaatcc
    23821 aatcaaggac ggcagtcata aagaagatcg gttgcggcat tggtttattg cccaggacat
    23881 tgctgcgctg gaagatgaat atggtcgatt gccgatggta aataaaacaa acgataccta
    23941 caccgtcgaa tacgaaacgt tcatccccgt tttgactaaa gccattcagg aaatggccgc
    24001 aagaattgaa acattagaaa ccgaaatgaa ggaatcgaaa aaatgacaag atgtgtgatt
    24061 gatcaagacg gcttgttcgt agaggaacag tattttgatg acggccgcca aagcatcgaa
    24121 gctgaaatgc ctgatctcgc acaatatcag gccgcccagt gggatggaca aggttggaag
    24181 ataattcccg actatcgcgg atgcgtagtt tttgccggtg ggcaagagca ggtgtgggat
    24241 aagttaggtg atttgcccga tggcgtcagc ctgaccccgc ctgaatcggt aaatattgac
    24301 ggcttaaaat ccgtaaaact cgtcgcatta aatgctgccg ctcaggcttt tattaacaag
    24361 cacgccggta tcgacagcgt acctgaattt gagtttgcaa gctgggcaat tcaagccgct
    24421 gaagcgaagg cttggcagga agataaagcc gcgccaacgc cagtgcttga cggcattgcc
    24481 accgcccgcg gtatgtcagc agacacgctt aaagcagcgg ctttgcgtaa aacgctggct
    24541 tacgaacaac tcgccgcaca tgtggcaggt caacgacaag cgctgcaaag caaaatcgaa
    24601 gcagcgaaaa cgcaggccgc gcttgataag attgcagtcg tattcacact gccggaggcc
    24661 gtctgaatgg ttcaagtcta tttggcactc tataaaggca aagccgcaat caacaccccg
    24721 cgcgatgtgg ttaaacgcat tgccgacagc gttgtacgat tggcaacatg cagcccgtac
    24781 agccattgtg aaatcgctgt taagcaccca cgcgacggcc tgtttgattg ttattcgtct
    24841 agcgcgagag acggcggggt gcgcattaaa accatgccgc tgcctgctga taaatgggac
    24901 ttaatcccgc tgccgcaatc tgttgccata tcggccagcc gcttgttcca ccgtacacac
    24961 ggtgcaggtt acgactggct aggtgcgatt ggcgtggtac tcaaatcacc acacagcaaa
    25021 agccgctggt tttgcagcga atggtgcgca tatgtaatag gctacactaa cccgtgccga
    25081 tacagcccgc aaaccctgta tgccgcggta tcaactaaag ataggccgtc tgagaaaatg
    25141 gaggaaacaa agtaatttag aaagttttaa ataaagagga gcggcgacgt gtctgtgttg
    25201 cgagcaccgg cacgccagcc aagcagatgt accctgcatt gacttcaagg ccgctttgcc
    25261 tagctaggcg gcggtaattc taacctaaac ggagttaatg cgacatggtt tattatcgtg
    25321 aattacgttg tgtctactgc aaaaaaacta ttggccaaag gcagcggtaa cgtacaaatc
    25381 aagtgtaacc gttgtaaaac ggttaatact ttcagctaga caaccattac taagaatgcc
    25441 gtcgagcatc attttaaaac tgatttcaga acaccagcga gagtgtcgga gagtaagtaa
    25501 aaatgatgca aaaataccac tcaacggccc cgctgccatt cgtcggacag aagcgatatt
    25561 tcattaaaca cttcactaaa gtattgtcgc aaattcccgc tgacggcaaa cattggacaa
    25621 ttgtagacgt attcggcggc agcggcctgt tggcgcacgt tgcaaaacgt atcaaaccgc
    25681 aggcgcgggt aatttacaac gactatgaca actactcaga ccgcctgcgg cacatcccag
    25741 attacaaccg tttgcgtgaa caaatcgcgc agatagttgg cggcatcccc aaaggctcaa
    25801 ggctagaccc tgaacgtacc cgatcagtgc aacaaacaat cactaatttc caaggccaca
    25861 ttgatgtgcg cgtactttca tcatggcttt tatttagtgc caaacaggca aattcgcttg
    25921 aacaattgct ggggtttgag ttctacaata aggtacgcca atccccatac tccatcgctg
    25981 ccgactattt agacggcctc gaaatcaccc agcaagacta taatcttttg atggctgagc
    26041 atcagcataa ccccaatact ctgctggtat tagacccgcc ttatgtgtcc actgctcagg
    26101 gtgcgtatgc cgctgataaa tactttaata tggttagctt cctgcgtatg attcagtata
    26161 tgcgcccacc gtttatccta tttagctcca cccgtagcga ggcgctggac tacttccaat
    26221 ttttgcaaga gtgcgaaccg gacaaatacc ggcgcttcag cggctacaat atcgtttcac
    26281 tagatgccaa gatgggcaaa ggaatcgagt atcaggacaa tatgatttat aaaatagatt
    26341 aacccgccta aacggcgggc ggtatcaaag ttccacctta tagcgcggcc taactaggtg
    26401 tcaaagattt tccgaaaagg tgctaaagtt cgcgccgcct tataaactgc acccgttttc
    26461 gggtgcagtt ttttattgcg gattgggacg ggaatggaaa aggatgtgga ttttttattt
    26521 taatcggcta tacaattgct gctttcatgc cgtctgaagc acgtttcaga tggcatcggt
    26581 ttacatcaga taaggataaa aatgggtatt tacgattttc aaatgaaaga tgcagaaggc
    26641 aatgcggttg atttgtcagg ctatcgcggc aaggttctgc tgattgtcaa cacggcaacg
    26701 cgttgcggtc tgaccccgca atacgaggct ttgcagaagc tgtatgcaca atataccgca
    26761 gaaggcttgg agattttgga ttttccgtgc aaccagttcc gcgaacaggc tcccgaaagc
    26821 agcggagaaa ttgcccaagt gtgtatgatg aagtttggca cgaagtttaa gattttcgac
    26881 aaaatcgaag tcaatggagc aaataccgcg cccttgtatg cctacctgaa atccgtcaaa
    26941 ccgcaagata aaggcaatca tctgtttaaa gatttcgtgt tgaagctcgc agctttggga
    27001 gagaagcgcg acgaaggcga catcaaatgg aactttacca aattcctcgt caaccgcgac
    27061 ggcgaagtgg tcgagcgttt tgcgcctagt gttacgcccg aagaaatcga agccgatatt
    27121 cgggcattgc tgtaatctgc cgccgtcttg ggaaatgccg tctgaaggct tcagacggca
    27181 tttgtccgtt tgcgggttta ttccgaaata tcggcaacac cgctttcggg gaagatgggc
    27241 aaatccggcg gtgggagcag ggtatccaaa gtcagggcgg acgcatcgag tgtggggtag
    27301 ccttcaaacg cgaccgaata accgctgttt ccgttcatac ggattcgggc gtgcgcgccc
    27361 agagggaaag tgattttgtc ggcaatgtgc ccgaagggaa agcccgtcag cacggggatt
    27421 ttcgccgtgc gcgaaacatg gttggcaacg gcggaaaaat cataagacgg atcatagaca
    27481 tctcgaattt tttccatacg gaaattgccg aacacgatgg cgcgctgttt cttcaaaata
    27541 cccgaaagat acagcgtatt gagcatacgt tcgatgcggt agggctgttc gccgacatct
    27601 tcgaggaaca aaatgccgcc gtcgatgtcg ggcatataag gcgtgccggc gagcgaggcg
    27661 aggacgctta agttgccgcc ccacaatatg ccttcggttt cgacatcggc gcgttggata
    27721 taaggaacat caacggtcag gcggttttgg gttgcaccct tgataaaggc atccatcgta
    27781 aacgcaccgg gggcgggttt gccaaaatcg ctataagcca tcgggccggc aaaactcatc
    27841 atattgcctt ttgccaacaa tgccagctgg acggcgcata cgtcgctgaa tccgaaaaac
    27901 agcgtgccgt gttcgcgcat ccttgcgccg agcgaagcaa aatcgatatg cggcagaatg
    27961 cgtgccgcac cataaccgcc gcgcaagccc atcagcactt taggtgtggc aacgcgaccg
    28021 gaagcgacat cttggaaatc ggcggcacgt tgtgagtccg tgccggcaaa ccgttggaaa
    28081 cggcggctgc ccgcctgttg gttggttacg gtaaaaccgg cattgtaaag gcgggttaag
    28141 gctgtgttga cgcggttggt gtcttcggca aagcctgacg aagcgacaac gcgcagaagg
    28201 ttgccgtccg aactttgacg gcgtggcgtt tggagaggca cggttcgggc tttcacaacg
    28261 gaatggaaag agggaagggg cggaacggat gtggcggatg tgccgcaagc ctgaagcagt
    28321 cccgcgccgg cggcggcggt gcaggttttc agaaaacgac ggcgggaggt cggttcggtc
    28381 ataatttccc tttcatacgg tttcagacgg catcggcggg cttaaagcag ctttttaagc
    28441 tgttccgtcc aatcttgcgg cagggtcata ccgtgttttc ggacggggtt tgcccaaatc
    28501 ggcgcaggga aggacgcatc gttttcaaag cgggcgataa tatgccaatg caggtgcggc
    28561 acgacattgc ccaagctggc gaggttaatc ttggcaggtt gaaacacttg gcgcatagcg
    28621 gcttcgactt tgtacaccat ttccatcaat tcgccgcgtt ccgctgccga aaggtcggtc
    28681 atttcggcaa tatgcttacg ccaaatgacg cggcagaatg caggcgaacc gctgtcgtta
    28741 tggacggcga tgacgcgcag cttgggggtt tgcagcaaaa tatcttcatc ttgtgccgtg
    28801 cagatggggc agggttgggc ggtcattttt ccttattcct gaaagtgttt aaaaaacggt
    28861 aggataattt c
//
LOCUS       DAWKOG010000007        27763 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.007, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000007 DAWKOG010000000
VERSION     DAWKOG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27763
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.007"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..386)
                     /locus_tag="WJK66_000249"
     CDS             complement(<1..386)
                     /locus_tag="WJK66_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HEZ7578021.1"
                     /translation="MYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQRYASFWGDG
                     YDVYRQLRWQQIPEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAMELK
                     AFPCLSSLFLTKWHKNLYDSGLTKTST"
     gene            complement(663..1451)
                     /locus_tag="WJK66_000250"
     CDS             complement(663..1451)
                     /locus_tag="WJK66_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ7578022.1"
                     /translation="MNPQDKCWQVHRHLAEHADQRLTLVRNAPKHILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAVRKGGFWQRFTGKGVVQHCQSPTAPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWARALETDGLLFFTCFGRDTLAELKCRLKENGIESRSA
                     LFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAMSWNDENAA
                     RSCVGTIFEREGGLSITLETVYGHAVKKLMLPQGENVVQFFPKR"
     gene            complement(1453..2367)
                     /gene="bioH"
                     /locus_tag="WJK66_000251"
     CDS             complement(1453..2367)
                     /gene="bioH"
                     /locus_tag="WJK66_000251"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HEZ7578023.1"
                     /translation="MCLKHRGQLFAASVRYAAKSAAQPAQMPSETPDEPWHNTQRLIV
                     GASAMRRQQERKSMPDAVKKVYLIHGWGANRHVFDDLMPRLPATWPVSAVDLPGHGDA
                     PFAQPFDIEAAADAVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFARL
                     TAAEDYPEGLAAPALGKMVGAFRTDYAKHIKQFLQLQLLHTHDADRIIGRILPDLARC
                     GTPSALQEALDAAERADARHLLDKIDVPVLLVFGGKDAITPPRMGEYLHRHLKGSRLV
                     VMEKAAHAPFLSHAEAFAALYRDFVEGV"
     gene            2423..2920
                     /locus_tag="WJK66_000252"
     CDS             2423..2920
                     /locus_tag="WJK66_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ7578024.1"
                     /translation="MWASLHYEPPVSNMIHALKHLADLSMVQPLADLMMQNPPDRLAD
                     ECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWQILPRHTVFRHHRPPQSTLKG
                     GERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELAKTLKKLGANRICCWTLAR
                     TPMKK"
     gene            2985..3449
                     /gene="trmL"
                     /locus_tag="WJK66_000253"
     CDS             2985..3449
                     /gene="trmL"
                     /locus_tag="WJK66_000253"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEZ7578025.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVYENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            3460..3582
                     /locus_tag="WJK66_000254"
     CDS             3460..3582
                     /locus_tag="WJK66_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ7578026.1"
                     /translation="MPSENLSGHIPNRRPHCTAVRNVSGGHDAHSSSTQERNTF"
     gene            3579..4298
                     /locus_tag="WJK66_000255"
     CDS             3579..4298
                     /locus_tag="WJK66_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEZ7578027.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHTSANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVSQGTAHVKIEQIVPGKSAPV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNFAASTSSPNLAIEKRRYEYVVKMGPFASQ
                     ERAAEAEAQARGMIQTVLTAG"
     gene            4352..4678
                     /locus_tag="WJK66_000256"
     CDS             4352..4678
                     /locus_tag="WJK66_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ7578028.1"
                     /translation="MNFKRLLLTAAATALMGISAPALAHHDGHGDDDHGHAAHQHNKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            4760..5344
                     /gene="ruvA"
                     /locus_tag="WJK66_000257"
     CDS             4760..5344
                     /gene="ruvA"
                     /locus_tag="WJK66_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEZ7578029.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVSGIGAKTALGILSAMTADELAQAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAAAPAADETEDIVSTLLA
                     LGYSEREAKAAVKGVPEGTDVGEGVRLALKNLLK"
     gene            5344..5481
                     /locus_tag="WJK66_000258"
     CDS             5344..5481
                     /locus_tag="WJK66_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578030.1"
                     /translation="MPSEGGAAFALTKPCVSAPRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            5507..7363
                     /locus_tag="WJK66_000259"
     CDS             5507..7363
                     /locus_tag="WJK66_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ7578031.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMTGVRKWIA
                     ALAALTAGIGIMEALVFQFMGKIVEWLGKYAPTELFAEKGWELAAMAAMMVFSVAWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VGDGMATLSKPHTILDKPNALPLKVPQGAIKFEHVDFSYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGITQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPELSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            7368..8291
                     /gene="rsgA"
                     /locus_tag="WJK66_000260"
     CDS             7368..8291
                     /gene="rsgA"
                     /locus_tag="WJK66_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEZ7578032.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIRAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENAGSLRPALQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAETRAASPERLAFLQGITDELPG"
     gene            8318..8494
                     /locus_tag="WJK66_000261"
     CDS             8318..8494
                     /locus_tag="WJK66_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII"
                     /protein_id="HEZ7578033.1"
                     /translation="MPSETGFGFQTASVFQKCYNPLFTGTSETMFQHAGRHLKHRPMC
                     RPDLEGLTPLPNKV"
     gene            8555..8779
                     /locus_tag="WJK66_000262"
     CDS             8555..8779
                     /locus_tag="WJK66_000262"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEZ7578034.1"
                     /translation="MKKNAPKSFEEALSRLESLTQAMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDADGLKELNLESDE"
     gene            8763..9659
                     /locus_tag="WJK66_000263"
     CDS             8763..9659
                     /locus_tag="WJK66_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEZ7578035.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSENEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGGVVADAVGQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHVQYDEATALLVGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGAMSCPNLSEDSLAVLDDYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(9768..10067)
                     /locus_tag="WJK66_000264"
     CDS             complement(9768..10067)
                     /locus_tag="WJK66_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HEZ7578036.1"
                     /translation="MTASMYILLLLALIFANAPFLTTKLFGIVPLKRKHFGHHLIELA
                     AGFALTAVLAYILESRAGAVHDQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(10179..11624)
                     /gene="nuoN"
                     /locus_tag="WJK66_000265"
     CDS             complement(10179..11624)
                     /gene="nuoN"
                     /locus_tag="WJK66_000265"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEZ7578037.1"
                     /translation="MNWSDLNLMPAMPEIVLLALLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFALFVYARPYNQVRG
                     IFKGEFYTLSLFALLGMGVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKIAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGHVWLSVFAVVMSLIGAFYYLRVVKVMYFDESGRARPTVGGNNAAKF
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            complement(11634..13130)
                     /locus_tag="WJK66_000266"
     CDS             complement(11634..13130)
                     /locus_tag="WJK66_000266"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEZ7578038.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLATGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFVAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AVIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNSHVAEM
                     KDINCREFAILAVLAVAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKM"
     gene            complement(13219..15243)
                     /gene="nuoL"
                     /locus_tag="WJK66_000267"
     CDS             complement(13219..15243)
                     /gene="nuoL"
                     /locus_tag="WJK66_000267"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEZ7578039.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFLNGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHDDHHGEEHHSLGKNDNPHESPLVVTLPLILLAIPSVIIGYVAI
                     EPMLYGDFFKDVIFVNADAHPTMHIMKEEFHGALAMVSHSLHSPVLYLAIAGVLSAWL
                     LYVKLPHLPAKIAQTFRPIYVLFENKYYLDALYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            complement(15292..15633)
                     /locus_tag="WJK66_000268"
     CDS             complement(15292..15633)
                     /locus_tag="WJK66_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578040.1"
                     /translation="MMDKNQLEQEFHKAMLNIYQEALNLPQPYKATRFLQIVNEFGGK
                     EAADKLLSTGEKKTQTGFTELILSGGGVHALKYSMEYLVLQKPWCDLFTEEQLAVARK
                     RLERVGFVFPK"
     gene            complement(15757..16332)
                     /locus_tag="WJK66_000269"
     CDS             complement(15757..16332)
                     /locus_tag="WJK66_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEZ7578041.1"
                     /translation="MPSENPIGKTMKSIDEQSLHNARRLFESGDIDRIEVGTTAGLQQ
                     IHRYLFGGLYDFAGQIREDNISKGGFRFANAMYLKEALVKIEQMPERTFEEIIAKYVE
                     MNIAHPFLEGNGRSTRIWLDLVLKKNLKKIVNWQNVSKTLYLQAMERSPVNDLELRFL
                     LKDNLTDDVDNREIIFKGIEQSYYYEGYEKG"
     gene            complement(16366..16671)
                     /gene="nuoK"
                     /locus_tag="WJK66_000270"
     CDS             complement(16366..16671)
                     /gene="nuoK"
                     /locus_tag="WJK66_000270"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEZ7578042.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDELKG"
     gene            complement(16668..17339)
                     /locus_tag="WJK66_000271"
     CDS             complement(16668..17339)
                     /locus_tag="WJK66_000271"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEZ7578043.1"
                     /translation="MTFQLILFYIFAVIILYGAIKTVTAKNPVHAALHLVLTFCVSAM
                     LWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTVNPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGLKPE
                     GEGKA"
     gene            complement(17375..18082)
                     /locus_tag="WJK66_000272"
     CDS             complement(17375..18082)
                     /locus_tag="WJK66_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578044.1"
                     /translation="MEGLINALKYLAEHEPIDNFEEIRTRNSPIELPSGLSNFEQNIF
                     LKENLSPKLQNDDSLKTHYWIIREWGGIKSFKQSAENSQLIRQFLSELNSGKLSSGLL
                     KISSLSKLASFIDCERFAIYDSRAIFSLNWLLFKFTNADLFFQPQGRNRELEIRNMNV
                     LFHFSDIKPNYRKPDVSFHQYCGLLQDLAKQVYGKQAKPYHIEMLLFKIATTWICADM
                     DQLIKFDCLRNQDFQTA"
     gene            complement(18113..18592)
                     /gene="nuoI"
                     /locus_tag="WJK66_000273"
     CDS             complement(18113..18592)
                     /gene="nuoI"
                     /locus_tag="WJK66_000273"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEZ7578045.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            complement(18672..19748)
                     /gene="nuoH"
                     /locus_tag="WJK66_000274"
     CDS             complement(18672..19748)
                     /gene="nuoH"
                     /locus_tag="WJK66_000274"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEZ7578046.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILISALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVILGLWMISPLNLWK"
     gene            complement(19751..22012)
                     /locus_tag="WJK66_000275"
     CDS             complement(19751..22012)
                     /locus_tag="WJK66_000275"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEZ7578047.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVDVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKTVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWVDVDWKTALEYVRSAIECIAKDGNQNQVGVWANPMNTVEELYLAKKLAD
                     GLGVKNFATRLRQQDKRLSDGIKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLASSKEELFMPLLSQEAAHPDEWAGRLKNLSANAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQAANSVGADVLNVNSGESV
                     AEMANAPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPMWKVLRVLGNLFDLKGFEYHDTAAI
                     LKDALDAESLPSKLDNRSTWTGEGVQTASDRLVRVGGVGIYHTDSIVRRSAPLQETSH
                     AAVPAARVNPNTLARLGLQDGQIAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMDTIELAGA"
     gene            complement(22302..22739)
                     /locus_tag="WJK66_000276"
     CDS             complement(22302..22739)
                     /locus_tag="WJK66_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578048.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICWKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINEL"
     gene            complement(22739..24040)
                     /gene="nuoF"
                     /locus_tag="WJK66_000277"
     CDS             complement(22739..24040)
                     /gene="nuoF"
                     /locus_tag="WJK66_000277"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEZ7578049.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DLEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIVRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGKMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
     gene            complement(24430..24903)
                     /gene="nuoE"
                     /locus_tag="WJK66_000278"
     CDS             complement(24430..24903)
                     /gene="nuoE"
                     /locus_tag="WJK66_000278"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEZ7578050.1"
                     /translation="MLSAESLKQIDTELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGITPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            complement(24903..26159)
                     /gene="nuoD"
                     /locus_tag="WJK66_000279"
     CDS             complement(24903..26159)
                     /gene="nuoD"
                     /locus_tag="WJK66_000279"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEZ7578051.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELDGEQIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYEGSKFRNAKVLKQLNESREGTMLDFIDVFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCADWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            complement(26149..26742)
                     /locus_tag="WJK66_000280"
     CDS             complement(26149..26742)
                     /locus_tag="WJK66_000280"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEZ7578052.1"
                     /translation="MASIQNLYETVSRVLGNQAGKVISALGEITVECLPEHYISVMTA
                     LRDHEELHFELLVDLCGVDYSTYKNEVWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            complement(26755..27237)
                     /locus_tag="WJK66_000281"
     CDS             complement(26755..27237)
                     /locus_tag="WJK66_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEZ7578053.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            complement(27228..27584)
                     /locus_tag="WJK66_000282"
     CDS             complement(27228..27584)
                     /locus_tag="WJK66_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEZ7578054.1"
                     /translation="MLSAYFPVFVFILVGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
BASE COUNT         6943 a         7760 c         6956 g         6104 t
ORIGIN      
        1 gtactggttt ttgttaatcc actatcatat agatttttat gccatttggt cagaaacagc
       61 gaagacaggc agggaaacgc cttcagttcc atcgcgtctt caaaatcatc ccaaacatcg
      121 ctcaaattct gtttggatat gccgtattcc cgtccggcaa acatcacggt ctttttgctt
      181 ttggcggctt ttttgaatgc ctttctctgt ttttcgggta tctgctgcca tctcaactga
      241 cggtacacgt cgtagccgtc gccccaaaaa gaggcatacc gttgcgtgtc ttcgccgacc
      301 agcggcgcgg tattcccaac ccgtacatat cccgtcggca cgacggcact tgccttatac
      361 atgtgcagac cgcgttccca atacataaag tctttgtaaa gcggtttcaa atccgccagc
      421 tccgccgccc caagcggttt gttgccctgc cagtccaaag ccagcagggt tgcatatgct
      481 tttttcccac cgtgttcttc aactcgggta acctggcagg cagtgtactg cttcagtctg
      541 aagttataga cgcaatatac gtctccggca tcggcataca tcggcatttc ctttatttct
      601 atcggttttc acagacagat gccgtctgaa acttggaaac agcttcagac ggcatctgca
      661 catcatctct tcggaaaaaa ctgcaccacg ttctcccctt gcggcagcat cagtttttta
      721 acggcgtgtc cgtacactgt ttccagcgta atactcaaac cgccttcccg ttcaaatatt
      781 gtcccgacac acgaacgcgc ggcgttttca tcgttccacg acatcgcccg ccaaacgccc
      841 agcgtgtcca tatccgccca aaacgtttcc gcctttttgt aatccaacac cagcttcgcc
      901 gtatcggtaa cggggtcgta aaacccgttt tcagcaagca tatcgcccaa gtcgtgcata
      961 tcggggaaaa gcgcgctgcg gctttcaatg ccattttctt tcagacggca tttcagttcc
     1021 gccaaggtat ctcgcccgaa gcaggtgaaa aacagcagcc cgtccgtctc cagcgcgcgc
     1081 gcccagttgt gcagcacagg aaggatttgt tccgccgcca acagtccgag attcgaccac
     1141 aacatatcgg cacacgcttc gggcagcggc gcggtcgggg attggcagtg ttgcaccacg
     1201 cccttacccg taaacctttg ccaaaaaccg cccttgcgca cggctgcggc agccgccaaa
     1261 aaatccgcac gggaatcgta ttcttcaaat accgcctgcg gatatcgttt cgccagcagg
     1321 ctgcggctga tgtccgcatc cgccccggca agcaggatat gtttgggcgc gttgcggacg
     1381 agtgtcagcc gttggtcggc atgttcggca agatggcggt gaacctgcca acatttgtcc
     1441 tgcggattca tgtcaaaccc cttcgacaaa gtcgcggtac agcgcggcaa acgcttccgc
     1501 atgactcaaa aacggcgcgt gtgccgcctt ttccatcaca accagcctgc tgcctttcaa
     1561 atgccgatgc agatactcac ccatacgcgg cggtgtaatc gcgtctttgc cgccgaacac
     1621 cagcagtacc ggaacatcta tcttgtccaa caaatgccgc gcatccgccc tttccgccgc
     1681 gtccaaagct tcctgtaagg cggagggcgt gccgcagcgc gccaaatcgg gcaggattct
     1741 gcctatgatt ctgtccgcat catgcgtgtg caaaagctgg agttgtagaa actgtttgat
     1801 atgtttggca taatccgttc ggaacgcgcc gaccattttg cccaatgcag gcgcggcaag
     1861 cccttcggga taatcttcgg cagccgtcag ccgtgcgaaa ctcgccgtca ggcagagcga
     1921 acggactttg tcgggatggc gagccgccag atacagcgcg accaatccgc cgagcgacca
     1981 gccgagaatg tcggcgggcg tatcgatttg tgcggcaaca gcgtcggcgg ctgcctcaat
     2041 atcgaagggt tgcgcgaagg gcgcgtcgcc gtgtccgggc aaatcgacgg cggacaccgg
     2101 ccacgttgca ggcaggcgcg gcatcaaatc gtcgaacacg tggcggttcg ccccccaacc
     2161 gtgtatcagg taaacttttt tgacggcatc aggcatggat tttctctctt gttggcggcg
     2221 catcgcagac gcgcccacta tcaggcgttg cgtattatgc cacggttcgt ccggcgtttc
     2281 agacggcatc tgcgccggct gcgcggcgga tttggcggca taccgcaccg acgcggcaaa
     2341 cagctgcccc ctgtgtttca gacacatcca aggcggcgcg gtgtgcggcg gctgtcagaa
     2401 aaaaccgccc gcattcgacc ggatgtgggc ttcgctgcat tacgaaccgc ccgtcagcaa
     2461 tatgatacac gcgctgaagc acttggctga tttgagcatg gtgcagccgc tggcagacct
     2521 gatgatgcag aatccgcccg accggcttgc agacgaatgt ttcgatttcg tcctgcccgt
     2581 accgctaagc agggagcggc tgctgcaacg cgggttcaac caaagtgaaa gcatcgtcgg
     2641 gctgctggca caacgctacg gctggcagat actgccccga cacaccgttt tccgacacca
     2701 ccgcccgccg caaagcacgc tcaaaggcgg cgaacggcgg cgaaacatca aaaacgcctt
     2761 tgaaatccgc acaccgatac cggaaaactg taatattctg ttaatcgacg atgtctttac
     2821 caccggcgcg acgctggacg aattggcaaa gacgctgaaa aaattaggcg caaaccgcat
     2881 ctgctgctgg acgctggcac gcacgccaat gaaaaaataa ctaaattttt tgacacccca
     2941 tccgccttgt ggcaaggtta cgcgcctata agtgattgat atttatgttt accatcgttt
     3001 tataccaacc ggaaatcccc ccgaacacgg gcaacatcat ccgcctgtgc gccaataccg
     3061 gcgcggattt gcaccttgtc aaaccgctcg gcttcccatt ggattccgcc aaaatgaaac
     3121 gcgccgggct cgactaccac gaattcgcca gcctgacggt gtacgaaaac ttcgacgact
     3181 gcctcaaggc actcgcaggc aggcgcattt tcgccctgac caccaaaggc acggcgcgcc
     3241 ccgatgaaac cgcgtttcaa aaaggcgacg ttttactgtt cgggccggaa acgcgcggac
     3301 tgcctgccga catcctcgac agcctgcccg ccgcgcaaaa aatccgcctg ccgatgcagc
     3361 ccggcagccg gagtatgaac ctttccaata ccgttgccgt gattctcttt gaagcgtggc
     3421 ggcaacacgg ttacgcaggc ggcgtttgaa cgcaagttca tgccgtctga aaacctatcc
     3481 ggacacattc cgaaccgccg tccgcactgt acggcggttc ggaacgtatc cggcgggcat
     3541 gatgcccatt cgtcttcaac tcaagaacgg aacacgtttt gactttaacc cgaaaaaccc
     3601 ttttccttct caccgccgca ttcggcacac actcccttca gacggcatcc gccgacgcag
     3661 tggtcaaggc agaaaaactg cacacctccg ccaaccgcag ctacaaagtc gccggaaaac
     3721 gctacacgcc gaaaaaccaa gtcgccgaat tcacgcaaac cggcaacgcc tcgtggtacg
     3781 gcggcaggtt tcacgggcgc aaaacttccg gcggagaacg atacgatatg aacgccttta
     3841 ccgccgccca caaaaccctg cccatcccca gctatgtgcg cgtaaccaat accaaaaacg
     3901 gcaaaagcgt catcgtccgc gtcaacgacc gcggcccctt ccacggcaac cgcatcatcg
     3961 acgtatccaa agccgccgcg caaaaattgg gctttgtcag ccaagggacg gcgcacgtca
     4021 aaatcgaaca aatcgtcccg ggcaaatccg caccggttgc cgaaaacaaa gacatcttta
     4081 tcgacttgaa atctttcggt acggaacacg aagcacaagc ctatctgaac caagccgccc
     4141 aaaacttcgc cgcttcgaca tcaagcccga acctcgccat tgaaaaacgc cgttacgaat
     4201 acgtcgtcaa aatgggtccg tttgcctcgc aggaacgcgc cgccgaggcc gaagctcagg
     4261 cgcgcggtat gattcagacg gtattgaccg ccggttgatg tcccgccttt acgtctttca
     4321 aacaccacca gatacttcgc aggagcaaaa catgaacttc aaacgccttc tcttgaccgc
     4381 cgccgcaacc gcactgatgg gcatttccgc ccccgcactc gcccaccacg acggacacgg
     4441 cgatgacgac cacggacacg ccgcacacca acacaacaaa caagacaaaa tcatcagccg
     4501 cgcccaagcc gaaaaagcag cgttggcgcg tgtcggcggc aaaatcaccg acatcgatct
     4561 cgaacacgac aacggccgtc cgcactatga tgtcgaaatc gtcaaaaacg gacaggaata
     4621 caaagtcgtt gtcgatgccc gtaccggccg cgtgatttcc tcccgccgcg acgactgaat
     4681 ttgatacaat ccgtgccgtc tgaagcccga accggtttca gacggcattt tgcacccgac
     4741 acttcaggat tcggcacaca tgatcagcag actgaccggc aaactggttg aaaaaaaccc
     4801 tccgcaaatc gtcatcgatg tcaacggtgt cggctatgag gccgacgtat cgatgcagac
     4861 cttctacaac ctgccgcccg tgggtgaaag cgtacaactg tttacccagc ttatcattcg
     4921 ggaagacgca catcttttat ttggttttgc cactgcggaa gagcgcaaaa cattccgcca
     4981 actgatcaag gtcagcggca tcggggcaaa aaccgctttg ggcattttgt cggcgatgac
     5041 ggcagacgag ctggcgcagg cggttgcaga agaagatgtc aaacgcctgt cttccgcgcc
     5101 gggaatcggc aaaaaaaccg ccgaacgtat ggtcttggaa ctgcgcggca agctggtcgc
     5161 gcatacggta acggacgggc tgtttgccgc cgcacccgcc gccgacgaaa cggaagacat
     5221 cgtcagcacg ctgcttgcgc tgggttacag cgaacgcgaa gcaaaagcgg cggtcaaagg
     5281 cgttccggag gggacggacg tgggcgaagg cgtgcgcctt gctttgaaaa acctgctgaa
     5341 ataatgccgt ctgaaggcgg cgcggcgttt gccctgacga aaccctgcgt ttccgccccg
     5401 cgctgtcttc ccgacggctt ttcccgtaaa atggcgttat tgtccttcct ttcagacggc
     5461 attgtgtttg atgccgtctg aaactgtttt accgaaatcg aaaacaatgt tgaacaaaat
     5521 attttcctgg ttcgagtccc gaatcgaccc ttatcccgaa gccgccccga aaacgccaga
     5581 aaaaggcttg tggcggtttg tctggagcag catgaccggc gtgcggaaat ggatagccgc
     5641 cctggctgcg ctgaccgccg gcatcggcat tatggaagcc ctggtttttc aatttatggg
     5701 caaaatcgtg gagtggctcg gcaaatacgc gcccaccgaa ctgtttgccg aaaaaggttg
     5761 ggaactggcg gcaatggcgg cgatgatggt gttttcggtt gcgtgggcgt ttgccgcgtc
     5821 caacgtgcgc ctgcaaaccc ttcagggcgt gttccctatg cgcctgcgct ggaacttcca
     5881 ccgcctgatg ctgaaccaaa gcctcggttt ttatcaggac gaatttgccg gacgcgtgtc
     5941 cgccaaagtc atgcagaccg cgctggcgtt gcgcgacgcg gtgatgacgg ttgccgatat
     6001 ggtcgtttat gtgtcggtgt atttcattac ctccggcgtg attctcgcct cgctcgactc
     6061 atggctgctg ctgcccttta tcggctggat tgtcggtttc gcttcggtga tgcgcctgct
     6121 gattcccaaa ttggggcaaa ccgccgcatg gcaggcggat gcccgctcgc tgatgaccgg
     6181 ccgcattacc gatgcctatt ccaatatcgc caccgtcaaa ctcttctccc acggcgcgcg
     6241 tgaagccgcc tatgccaagc agtcgatgga agaatttatg gttacggtgc gcgcccaaat
     6301 gcggctggcg acgctgctgc attcgtgcag cttcatcgtc aacacctccc tgaccctctc
     6361 caccgccgca ctgggcatct ggctctggca caacgggcag gtcggcgtgg gcgcggttgc
     6421 tacagccacc gccatggcgt tgcgcgtcaa cggtttgtcg caatacatta tgtgggaatc
     6481 cgcgcggctg tttgaaaaca tcggcaccgt cggcgacggc atggcaaccc tgtccaaacc
     6541 gcacaccatc ctcgacaagc cgaacgccct gccgttgaaa gtgccgcaag gcgcaatcaa
     6601 atttgaacac gtcgatttct cctacgaagc gggcaaaccg ctgctcaacg gcttcaacct
     6661 caccatccga cccggcgaaa aggtcggctt gatcggacgc agcggcgcgg gcaaatccac
     6721 catcgtcaac ctgcttttgc gcttctacga accgcaaagc ggcacggttt cgatcgacgg
     6781 gcaggacata agcggcatca cccaagaatc tttacgcgcc caaatcggtt tggtcacgca
     6841 agatacctcg ctgctgcacc gttccgtgcg cgacaacatt atttacggcc gccccgacgc
     6901 gaccgatgcc gaaatggttt ccgccgccga acgcgccgaa gccgccggct tcatccccga
     6961 actttccgat gccaaagggc ggcgcggcta tgacgcgcac gtcggcgaac gcggcgtgaa
     7021 actctccggc gggcaacgcc agcgcatcgc cattgcccgc gtgatgctca aagacgcgcc
     7081 gattcttctt ttggacgaag ccaccagcgc gctcgattcc gaagtcgaag ccgccatcca
     7141 ggaaagcctc gacaaaatga tggacggcaa aaccgtcatc gccatcgccc accgcctctc
     7201 caccatcgcc gcaatggaca ggctcgtcgt cctcgacaaa ggccgcatca tcgaagaagg
     7261 cacacacgcc gaactcctcg aaaaacgcgg gctttacgcc aaactctggg cgcaccagag
     7321 cggcggcttc ctcaacgaac acgtcgagtg gcagcacgac tgaaccgatg ccgtccgaac
     7381 acccgttttc agacggcatt tccacaccca accccaaaga aaccatgaac gacaccgccc
     7441 aaattaccgc cagctacggc cgccgctaca tcgtccgcac gcccgacggc acaacctacg
     7501 aagccagcac ccgcaaaaaa cgcgtcgatt tcgcctgcgg tgaccgcgtc cgcatcagcc
     7561 ccgtcaacgc cgaacaagtt gtgattgaag attttttacc gcgccaaagc ctgctctacc
     7621 gccaagacgc gtggaaaacc aaactcattg ccgccaacgt tacccaactc ctcatcgtaa
     7681 ccgccgccgt cccgagtccg agcgtgcggc tgctacagcg cgccctgctt gccgccgaag
     7741 ccgccggcat ccgcgccgtc atcgtcctga acaaagccga cctgcccgaa accgcccttt
     7801 ggcgcgaaaa actcaaattc tacgaaacgc tgggttatcc cgtcatcgaa acccgcgcac
     7861 tggaaaacgc cggcagcctg cgccccgccc tgcaagggca cagcaacatc ctgctcgggc
     7921 agagcggtat gggcaaatcc accctgacca acgccctttt gggcagccaa accgcccgca
     7981 ccggcgacat ttccgccgca ctcgactcgg gaaaacacac caccacccac gcccggcttt
     8041 atgatttgaa cggcgaaacc caactcatcg actccccggg tttgcaagaa tttggtttac
     8101 accacctcca agccgccgat ttgccgcgct atttccccga tttccgccac cttgtcgggc
     8161 aatgccgctt ccacaactgc acccaccgcg ccgaacccgg ctgcgccttc aaagccgccg
     8221 ccgaaaccag ggcggcaagc cccgaacgcc tcgccttttt gcagggcatc accgacgaac
     8281 tgcccgggta acgccttgcc gtttgcagga caaaacaatg ccgtctgaaa ccggattcgg
     8341 gtttcagacg gcatccgttt ttcaaaaatg ctacaatccg ctttttaccg gaacatccga
     8401 aactatgttc caacacgcag gacgacactt aaagcaccgc cctatgtgtc gtcctgattt
     8461 ggaagggctt acacccctcc caaataaagt ctgattctac cgccccgaag gacagatgtc
     8521 caagtggcgg ggtttcaacc gaaaaggaaa cacgatgaag aaaaacgccc cgaaatcgtt
     8581 tgaagaagcc ttgtcgcgcc ttgaatcgct gacacaagct atgcagggcg aaatgccctt
     8641 ggaagacgcg cttgccgcct atcaggaagg caacgagctg gtcagatact gccaaaccaa
     8701 gctggcgcaa gtcgaacaga aattacaggt tttggatgcg gacgggctga aggagttgaa
     8761 ccttgaatcc gacgaatgat ttgaaagcgt ggcaacagag ggcgcaggca cagacagagc
     8821 tgctgctgga acgctttttg ccgtctgaaa acgaaatccc gcacacgctg cacgaagcga
     8881 tgcgctatgc ggctttggac ggcggcaagc gtctgcgtcc gatgctggtg ctggcggctt
     8941 cggaattggg cggggtcgtg gcggatgcgg tcggacaggc aatggcggca atcgaaatga
     9001 tccacgtcta ttctttggtt cacgacgata tgccggcgat ggacaacgac agcctgcggc
     9061 gcggcaaacc gacttgccat gtgcaatacg acgaagccac cgcgctactg gtcggcgacg
     9121 ctttgcaaac ccaagccttc gacgtgttaa gccgtccgac agaactgccc gcagcacgcc
     9181 agttggcaat gttgtcggtg ttggcgaaag cgggcggcag cgcgggcatg gcgggcggtc
     9241 aggcaatcga tttggcgaat gtcggcaaac aaatggttca agccgatttg gaacagatgc
     9301 acagcctgaa aacgggtgcg ctaatccgtg cggcggtttt attgggggcg atgtcctgcc
     9361 cgaatctgtc tgaagacagt ctggcggtat tggacgatta tgcggcaaaa ctggggctgg
     9421 cgttccaagt cattgacgat gtgttggatt gtgaagcgga cacggcgact ttgggcaaaa
     9481 cggcgggcaa agacgcagac aacgacaagc cgacttatgt gaaactgatg ggcttggaag
     9541 cggcgcgctc atacgcacac aaactggttg ccgaagcggt cgcgctgctc gaacccttcg
     9601 gcgacaaagc cctgcgcctg cggcagttgg cagaatttgc agtcgcccgc aaatattaaa
     9661 accggcgtat atcgccggct gaaacgtgcc ggcacagcga tgccggcctg cacttgaatg
     9721 aaacaaatgc cgtctgaaca ggtttcagac ggcattccta tgcctgccta ttccctgttg
     9781 cgcgtgtgcc aaaaataccg ccacacgaaa cagggaaacg caaaaatcag gtacaggcag
     9841 acgacggtgg cgtaaaactc ccaaccctga tcgtgtaccg ctcccgcacg ggattcgagg
     9901 atgtaggcaa gaacggcggt cagcgcgaaa cctgccgcca gctcgatcag gtggtgtccg
     9961 aaatgtttgc gcttgagcgg tacgatgccg aacagcttgg tcgtgaggaa gggggcgttg
    10021 gcaaaaatca aggcaagcag caaaaggatg tacatggatg cggtcatggc gggctttctg
    10081 tttgggcatt gtcggcggcg atgccgtctg aactgcgttg cggaacggca tatcttaaca
    10141 aaaacggcag cctctgacac ggctgccgtt gcggcggctt acagcgtgtt ttccaacgcc
    10201 ttggcgcacc agtcgataac ggtttgcggc ataatgcccc acagcaccaa cagcagcgcg
    10261 ttgacgctca aaaggaattt tgcggcattg ttgccgccga cggtgggacg ggcgcggccg
    10321 gattcgtcaa aatacatgac tttgaccacg cgcaggtagt agaacgcacc aatcagcgac
    10381 atcacgacgg caaatacgga caaccaaaca tggccttgtt tcaagagcgc cataatcacg
    10441 ccgaatttgg cgtaaaagcc catcagcggc ggaatgcccg ccatagagaa cataaccagc
    10501 agcatcaaaa aggcaagcca tacgcggtgt tggttcaacc ctgccaaatc gctgatgttt
    10561 tcgcactcgt tgtccccgtc cgacaacacc atcaacacac cgaaccctgc cgccgccatc
    10621 agcgcgtagg taatggcgta atagagaccc gccgcaaagc cgaccgcgcc cgccataaac
    10681 gccagcagga taaaccccat atgcgatacg gtggaatagg cgagcatacg tttgatattg
    10741 gtctgcatga tggcggcaag gttgccgacc agcagcgagg cggcggcaag cagggcaaac
    10801 atcagagacc agtcatgatg cacggttccc aacccggtaa cgaggatgcg gaaagcgaaa
    10861 acaacggcgg cgattttcgg ggcagtgccg accagggcgg taacagaagt gggcgcgccg
    10921 tgatacacgt cgggcaccca catatggaac ggcaccgcgc cgagtttgaa cgcaacggcg
    10981 acgacgataa acaccagacc cagtttcaac agccattcgt ttgcttcttc attgaaggaa
    11041 gaggcgagca cgccggcaaa ttccagcgaa ccggttgcgc cgtaaaccat agaaataccg
    11101 tagagcagca ggccggatgc cagcgcgccc agaacaaaat atttcaaggc ggcttcggcg
    11161 gcaaagccgg aatcgcggcg cagggcaatc agggcgtaaa gggcaagcga caagagttcc
    11221 aaaccgatat aggcagttaa aaaatgcccc gcgctcacca tcacgcccat acccaagagg
    11281 gcaaacagcg acagggtgta aaactcgcct ttgaaaatac cgcgcacttg gttgtagggc
    11341 ctggcataga caaacagggc aaaggtcaag gcatataaaa ccatttttgc caaacgcgac
    11401 ataccgtctg caatatacat cccgttgaac gaagacgtgc tgccctgttc ccacaccgcc
    11461 aactgcacca cagccgtaac cgccaccgtt gccaacgcgc cgtaatgcgt ccaacggcgt
    11521 ttgtcatcac tgacccacaa gtccgccagc aacaataaca ccagcagcgc aagcagcacg
    11581 atttcgggca tggcgggcat taaattcaaa tcagaccagt tcatttacac acctcacatc
    11641 ttgctttgtg ccacatgggc aatcaaatcg tttgccgcct gatgcaccac ttcgataaat
    11701 gcgttcggat acaggcccat acccaaaaca gcaaccgcca acaccgccaa aatcgcaaat
    11761 tcgcggcaat tgatgtcttt catttcggca acgtgcgaat tgtggatcgc accaaaaata
    11821 acgcgtttgt acatccacag ggtataagac gcgccgtaaa tcagggtcat ggccgccaac
    11881 gcgcccaccc agaaattcac tttgaccgcg cccataatca ccataaactc gcccacgaag
    11941 ccggaagttg caggcagacc ggcattggcc ataccgaaca acatcataaa cgccgcaaac
    12001 ttaggcatca cattgaccac gccgccatag tcagcaatat tgcgcgtgtg caggcggtcg
    12061 tacatcacgc cgatacacat aaacatcgcc gccgacacga aaccgtgcga aatcatctga
    12121 atgaccgcgc ctttcaacgc ccagtcgtcc aactgcccgt caacaaacaa aaacatccca
    12181 agcgttacaa aacccatatg gctgatggac gaatacgcca ccagtttctt catatcggtt
    12241 tgcaccaaag ccaccatacc gatgtaaatc acggcaatca ggctcaatac gatgatcacg
    12301 ggggcaaaat agcgtgccgc atccggcata atcggcagga taaagcgcaa gaaaccatac
    12361 gcacccagtt tcagcgtaat tgccgccaac accatcgaac cgccggtcgg cgcttcaacg
    12421 tgggcatccg gcaaccaagt gtgcacaggg aacatcggca cttttacggc aaatgacagg
    12481 aagaatgcca caaacaaaag ctgttgtacg cccaatggaa tctgcttgat gttttggaaa
    12541 tcgacaatag agaagctgcc tgtctgatag tacaggtaaa ccatggcaac cagcatcaaa
    12601 agcgaaccca tcagcgtata gaggaacagc ttgaccgacg cataaacgcg gcgcgggcca
    12661 ccccatacac cgataatcag gtacagcgga atcagcatac cctcgaagaa cacataaaac
    12721 agaatcgcat cctgcgcggc aaacgcgccg ttaatcaaac ccgacatcat caggaatgcc
    12781 gccatatact gcgccggacg tttctgaatg acttcccaac ctgccaatac caccaacagc
    12841 gtaataaacg cattcaagat gataaagagc actgaaatac cgtccacgcc caatgcgtag
    12901 ttgattttca gaagcggaat ccactcgtgg aactcggtaa attgatagcc gccgctcaaa
    12961 cggtcgaaac cggtaaacag gggcagtgtt accaagaaac cggcaagcgc acccatgaag
    13021 gcgagcacac gcgccagcgg cgcacggctg tctttaccgg tcgccaaaac cagcacacct
    13081 gcggcgatgg gtatccatat tgccaagctg agtaggtagt tggaaaacat agtggttaac
    13141 ctgtggttaa aataaaaata tcgttgtacg ggttggtttg tcagacgacg ttttaagaga
    13201 ccgtctgaaa cctcatcctt atctgaacaa tccccagaag gtcataccga gcaagaccaa
    13261 tacgccgaac accatagcgg cggcgtaggt atagataaag ccggtttgag ctttacgcac
    13321 ttgcgcggca atcgcgccga ccagtttggc agagccgttg acgatgccgt tgtcgataat
    13381 ggcggtatcg ccgactttcc agaagaaagt acccaatgcg cgtgtgcctt tggcgaaaac
    13441 gttgaaatac agggcgtcga ggtagtattt gttttcaaac aaaacgtaaa tcggacggaa
    13501 cgtctgtgca attttcgctg gcaggtgcgg cagtttgacg tacaaaagcc atgcgctcaa
    13561 cacgcctgcg atagcaaggt agagtacagg cgaatgcagg ctgtgcgaca ccattgccaa
    13621 tgcgccgtgg aactcttcct tcatgatgtg catggtcgga tgcgcgtcgg cgttgacgaa
    13681 aatcacatct ttgaagaaat cgccgtaaag cataggctcg atggcaacgt agccgatgat
    13741 gacggacgga atggcaagca aaatcaaagg cagggtaaca accaacgggc tttcgtgcgg
    13801 attgtcgttt ttgcccaagc tgtgatgttc ttcgccatga tgatcgtcat gatgttcagg
    13861 caagctgcgc catttctctt cgccgtggaa caccataaag tattggcgga acgcgtagaa
    13921 cgcggtaacg aacacgctgg caaggacggc aaaataggca aagccgctgc ccggcagggt
    13981 gctgtatttc gccgcttcga taatcgaatc tttagaatag aagccggaga agaacggcgt
    14041 accgatcagc gacaagttac cgatcagcat ggtcagccaa gtaatcggca tgtatttttt
    14101 caggttgccc atgtggcgca tgtcttggtc gtggtgcata ccgataatcg cgctgcctgc
    14161 cgccaagaac aacaaggctt taaagaaagc gtgggtcatc acatggaaca tcgccacgga
    14221 ataggcagac gcacccaaag ccacggtcat atagcccagt tgcgacaagg tggaatacgc
    14281 aaccacacgt ttgatgtcgt tttgaatcac gcccaagaaa cccataaaca gggcggtaat
    14341 cgcaccgatc accataatga cagacagcgc agtgctgctc atctcataaa tcggcgacat
    14401 acgcgatacc ataaacagac cggcggtaac catcgtcgca gcgtgaatca atgcagaaat
    14461 cggggtcggg ccttccatcg aatcaggcag ccaaacgtgc agtgggaatt gtgccgattt
    14521 acccatcgca ccgacaaaca ggagcaaaca ggtgacagta atcaacgacc attccacacc
    14581 ggggaaaagc tggatggtgg cattttgcac gttgggcaga taagcgaata catcttgata
    14641 gcgcaggctg ccgccgaaat aggcaagcac caagccgata ccgagcaaaa agccgaagtc
    14701 gccgacacgg ttgatcaaaa aggctttcag gttggcaaat gttgcgcttg gacgtttgaa
    14761 atagaaaccg atcaagagat acgacaccaa gcccacagcc tcccagccga agaagagctg
    14821 gatgaagttg ttgctcataa tcagcatcaa catgctgaaa gtaaacaaag aaatatagct
    14881 gaagaagcgt tggtagccga ctttttcatc gtgcatatag ccgatggtgt agatatgcac
    14941 catcaacgat acgcccgtta ccacgaccat catcatcgcc gtcatcgtat cgaccaagaa
    15001 gccgacggag aaatccaaac cgcccatcgt cagccaggta tagacgttct catcaaactt
    15061 ggcacggctg ccattgagga atccccacag cacataagcc gacagcacgg cagacaccgc
    15121 cacaccgagt atcgtaacag tatgcgcacc ggcacgaccg attttgttgc cgaacaaacc
    15181 cgcaatcaac gagcctgcca acggaacaag ggcaattatc aaatataaag tcatgtcgtt
    15241 catttgattg aatccgattg atttaaaaat ctatgtttgt ttcgtacaaa attacttcgg
    15301 aaaaacaaat ccaacacgct ccaatcgttt gcgtgccaca gctaattgct cttcagtaaa
    15361 taaatcacac cacggctttt gtaacaccag atattccata ctgtatttca aggcgtggac
    15421 tccgccacca ctcagaatca gctctgtaaa accggtctga gtcttctttt cccccgtact
    15481 taataattta tccgccgcct ctttaccacc aaattcattt acaatttgta aaaatcgtgt
    15541 cgccttgtaa ggttgcggca aattcaaagc ctcctgataa atatttaaca tggctttatg
    15601 aaattcttgt tctaactgat ttttatccat cattcttctt ccaatatttc agaccggatt
    15661 attcttaccc agaatttctt ttctcatccg ctcccgtctg atcacctacc gaatcaggtc
    15721 gtctgaaaca gtctgaaatc gcttttcaga cgaccctcag cctttttcat acccttcgta
    15781 ataatacgac tgctcgatac ctttaaagat gatttcacgg ttgtccacat cgtcggtcag
    15841 gttgtccttt aacagaaagc gcagttctaa atcgttgacg gggctgcgtt ccatcgcctg
    15901 caaatacagg gttttgctta cattttgcca gtttacgatt tttttcaggt ttttcttcag
    15961 caccaaatcc agccagatgc gggtacttct gccattaccc tccaaaaacg gatgggcaat
    16021 gttcatttca acatatttgg cgatgatttc ttcaaaagtc cgctcgggca tctgctcgat
    16081 tttaaccaaa gcctctttta aatacatggc gttggcaaaa cgaaaaccgc ctttggaaat
    16141 gttgtcttcc ctgatttgac ccgcaaaatc atataagccg ccgaacaggt aacggtgaat
    16201 ctgttgcagg cccgcggtgg taccgacttc gatacggtcg atgtcgccgc tttcaaacag
    16261 gcggcgggca ttatgcaggc tttgttcgtc tatggatttc atcgtttttc ctatcgggtt
    16321 ttcagacggc atcggtgtct tgtcaggtct cgacccaacc tacctttacc ctttcaactc
    16381 gtccaaatcg gcaacattaa tcgtttgtcg gttgcggtac accaatacca taatcgccaa
    16441 accgatggca gattcggcag cggcaacggt caatacgaag aatacgaaaa tttgtccggc
    16501 agtatcgccc aaatgttgcg agaaggcgat aaagttgaag ttcaccgcca agagcatcag
    16561 ctcgatggac atcagcaata ccagcacgtt tttgcggttc ataaagatac ccattgcgct
    16621 gataccgaac aggagcgcac ccaataccag ataatgcgtc aaggtaatca tgctttgccc
    16681 tccccttccg gcttgaggcc gtctgaaact tcgctctctt cggcagattc gacttgcggt
    16741 ttgaccgctt ccattttcac cagacgcata cggccttgat cggcgcgtac tttaacttgg
    16801 tcggcaggat ccatgcgttt cggattaacc gttttacggt gaaccagcgc aatcgcggcc
    16861 accataccca acagcagcaa taccgccgcc aattcaaacg gcagcagata atcggtataa
    16921 atacggctgc ccaaatcgcg gatattgttg taatcggcgg gaatgtcttt catcagccca
    16981 aatgcggcaa ggtcggtttt cggattgacc agaatcagaa tcagggcaac tgccaacaat
    17041 gtgccgacca caccggcaac aggcgcgtgc cgccagaaac cggcacgcat ttcttcaatg
    17101 tcgatgttca acatcatcac gacgaacagg aacaacacca tcacggcgcc gacgtaaacc
    17161 accaccagcg tcacgcccaa aaactcagcc tgcatcagca tccaaagcat cgcgctcacg
    17221 cagaaggtca acaccagatg caaagcggcg tgaacaggat ttttagcggt gacggttttg
    17281 atcgcgccat aaagaattat cactgcaaaa atataaaata aaatcagttg gaaagtcata
    17341 gtctatgctt tctttattaa tcaaaaatat ggtttcaagc agtctgaaaa tcctggttac
    17401 gcaaacaatc aaacttaatc agttgatcca tatccgcaca aatccacgtt gtcgcaattt
    17461 tgaataacaa catttctatg tgatacggtt ttgcttgttt accataaact tgtttcgcca
    17521 aatcttgtaa caatccacaa tattgatgaa acgaaacgtc tggtttccga taattcggtt
    17581 tgatatcaga aaaatgaaac aatacgttca tatttcggat ttctagttcc ctatttctac
    17641 cttgtggctg aaaaaacaaa tctgcatttg taaatttaaa caacaaccaa ttcaacgaaa
    17701 aaatagcgcg tgaatcataa atggcgaatc gctcacaatc tataaaagaa gccaatttag
    17761 ataatgatga aattttcaac aaaccactac tcaattttcc cgaattaagt tccgataaaa
    17821 attgacgaat aagctggcta ttttcagcag attgtttaaa acttttaatc ccaccccatt
    17881 cacggataat ccaataatgc gtcttcaagc tatcatcatt ttgtaatttt ggggataaat
    17941 tttcttttaa aaaaatattt tgttcaaaat tacttaatcc acttggcaac tcaatcggac
    18001 tatttctagt tctaatttct tcaaaattat ctattggctc atgttcggct aaatatttca
    18061 atgcattaat cagtccttcc atatttaaaa ccttccaaga agccccaaag cattaacgat
    18121 acggcgcgtc agcggctttg cgtttggcga tttcagcttc gtatttgtcg ccaatggcca
    18181 aaagaatcgg cttggtcatg tgcaagtcgc cttttttctc gccgtggtat tcaaaaatat
    18241 gggtttccac aatcgcatcg gtcgggcagg cttcttcgca gaaaccgcag aagatgcact
    18301 tggtcaggtc gatgtcgtaa cgcttggtgc ggcgcgtgcc gtcttcgcgt tcttccgatt
    18361 cgatgttgat cgccattgcg gggcaaacgg cttcacacaa tttacacgcg atgcagcgtt
    18421 cctcgccgtt tggataacgg cgttgcgcgt gcagaccgcg gaaacgcacg gattgcggcg
    18481 ttttctcttc gggaaaataa atcgtgtctt tgcgggcaaa aaagtttttg agcgttacgc
    18541 ccataccttt taccaattcg ccaagcagaa aggtttttac taagttagcc atattatgtt
    18601 ccctcaaaac agggattctg ttaaatattc aaaactgcgt tttcagacga ccttgatacg
    18661 tcgtctgaaa cttatttcca caaattcagc ggtgaaatca tccacagacc caaaatcacg
    18721 atgtaggcga agccgatcgg aatcagcact ttccagccca aacgcatgat ttggtcgtaa
    18781 cggtagcgtg ggaaagttgc gcggatccac agataccagt acaacaccgc cgccattttc
    18841 acgaacatcc agaatgcgga aggcgtgccg ataaagcccc agctttgtgg gaacggagac
    18901 aaccaaccac cgaggaacat caacgatgtc agcgcggaaa tcagaatcat gaaaatgtat
    18961 tcggcaagga agaacagcgc gaatgcgaag ccggagtatt cgacgtggtg gccggcaacg
    19021 atttcagact caccctctgc cacgtcaaac ggcgcgcggt tggtttcggc aacggcggaa
    19081 atcagataga cgatgaagat ggggaagagc ggcagccagt tccacgagaa cacggaaccg
    19141 cccgcaatac ctttggcttg cgcggcaacg atgtcggaga agttcatact gcccgatacc
    19201 atcacgacgc acaccagcgc ggcactcatg gcgatttcgt aagaaatgct ttgcgcggag
    19261 gcacgcattg cgcccaggaa cgaatatttg gagttggaag cccagcccgc gatgatcacg
    19321 ccgtaaaccg acagcgaggt aatcatcagg atgtacaaaa ggccgatatt gatattggtc
    19381 agcacccatt cttcgttgaa cggaatcact gcccacgccg caaaagacgg tgcaagcgac
    19441 ataatcgggc cgatgtagaa cagggctttg tttgacaact tcggacgggt tacttcttta
    19501 aacaagagtt tgaacacgtc ggcaaacggc tgaatcagac cccacgggcc ggttacgttc
    19561 gggccgacac gaagctgcat aaagccgatg actttacgtt cgaaatacgt caggtaggca
    19621 acggtcagaa tcagcggaat caggataatc acaattttga cgatgacgga taccaccaag
    19681 cctacggtaa tacccaaatc gcccagaccg agcgttgcgg caaagaggtt ttggaaccat
    19741 tcttgcatga tcaagctccc gccagttcaa tagtgtccat caacgcaccc agcgcggcat
    19801 tttcggtatg cagcggcaga tgcaccacgt tttcaggcaa accggcatcg gctttgacgg
    19861 caaccgatac gcttgcgccg ttttgtttgg cgacagcaat ttgtccgtct tgcaggccca
    19921 agcgtgccaa agtgttcgga tttacacgcg cggcaggcac ggcggcatgg ctggtttctt
    19981 gcaacggtgc ggaacggcgc acgatagaat cagtgtgata aataccgacg ccgccgacac
    20041 ggacaaggcg gtctgaggcc gtctgaacgc cctcccctgt ccatgtgcta cggttgtcca
    20101 gtttggacgg caggctttct gcatccagcg cgtctttcag aatcgcagcg gtatcgtgat
    20161 attcaaaacc tttcaggtca aacagattgc ccaatacgcg caatactttc cacatcggac
    20221 gcgaatcgcc gaagccttgt accacgccgt ggaaagattg cagacggcct tccatattga
    20281 tgaagctgcc tgaggtttcg gtaaacggtg caatcggcaa caacacgtcg cacacgtcca
    20341 gcagcgtttc gctgacaaac ggcgtaaacg ccatcacgct ttttgcctgt ttcaacgcgg
    20401 ctacggcttt tgcaccgtca accgtatcaa tttccggctc gacgttgagc aacaagactg
    20461 cctgtttcgg cgcgtttgcc atttcggcaa cgctctcgcc ggagtttaca ttcaagacat
    20521 ccgcaccaac actgttggcg gcttgcggca aaatacccag cactgcgccg gtcgcgtccg
    20581 ccagctcttg cgcggcggca taaatggcgg cgtaatcagg atggttttgc acttccgcgc
    20641 ccaaaatcac cgctgctttt tcagcgtttt tcaggctggc ggtaacggcg tgttccgcat
    20701 tggcagacag gtttttcaga cggcctgccc actcgtcggg atgtgcggct tcttgagaca
    20761 gaagcggcat aaacaattct tctttgctgc tggccaatac gctcaaggcc atacggtctt
    20821 tggcggcgcg gcgcaggcgg gcggtcagga gcggctgttc tttgcgcaag ttcgcgccga
    20881 ctaccaatac ggcatcgttg tcagccaaag attcaatgct ttgtcccaac cattgcgcac
    20941 ctttaatgcc gtctgaaaga cgtttgtctt gttggcgcag acgggtggca aagtttttaa
    21001 cacccaaacc gtcggcgagt tttttggcca gatacagctc ttcaaccgta ttcatcgggt
    21061 tcgcccaaac gccgacttgg ttttgattgc cgtctttggc gatacattca atcgcgctgc
    21121 ggacatattc caacgcggtt ttccaatcca cgtccaccca ctcgccgccc tgtttgattt
    21181 tcgggttttt cagacggctt tcgtgataca ggccttcgta ggcaaaacgg tcgcggtcgg
    21241 acagccagca ttcgttaatc gcttcgtttt ccagcggcaa cacgcggcgg acggtatggt
    21301 ctttggtttg tacaatcagg ttgctgccca aagcatcgtg ggcggaaaca gatttgcggc
    21361 ggttcaattc ccaagtacgc gcgttgaagc ggaacggttt gctggtcaat gcgccgacag
    21421 gacacaaatc gatgacgttg cccgacagct cggtttccac cgttttgccg ataaagggca
    21481 tgatttcgga gtgttcgccg cgattcacca tcgcaatttc ttgcaaaccg gcgatttctt
    21541 cagtgaaacg aacgcagcgg gtgcagtgga tacagcggct catttcctcg gcggaaacca
    21601 aaggccccat gtctttgccg acgacggaac gtttttcttc ggtgtagcgg ctggtggttt
    21661 ttccgtagcc caccgccaaa tcctgcaact ggcattcgcc gccctggtcg caggtcggac
    21721 aatcaagcgg atggttgatg agcaggaact ccatcacgcc ttcttgcgcc tctcgggctt
    21781 ttgccgaatg cgtacgcaca atcatgccgt ccgttaccgg cgtggcacag gctggcagag
    21841 gtttgggggc tttttccacg tccaccaaac acatacggca gttggcggcg atggaaagtt
    21901 ttttgtggta acagaaatgc gggatataag taccgagctt gtgcgcggct tcaatcaccg
    21961 tcgcgccctg ctccacagat acttgtttgc cgtcgatttc gatttgtaac atggttcgtt
    22021 cctagttacg ggtatttgat aaataattct ttatggagat cgtctgaaaa atgggtttca
    22081 gacggccttt tgagtttcat tgacaatcaa tactgtttaa aacgtttact gccaccttgc
    22141 cgtcattccc gcacaggcgg gaatccattc ttgggttttc ggtaactgct tttcaaatat
    22201 cgggttctgt aaattccact atggattccc gcctacgcgg gaatgacggc aaagttaaat
    22261 ttttagcatt ttgcttttaa ccaatataaa accaactaaa actataactc atttattata
    22321 tttttaaatg acttaaataa ctctttaaca gcacgctcat tgtcttcatc tgtaggctca
    22381 aacttttcac accaattaat tatatttaca tcgccatgca ataactctgc ataacatcct
    22441 actgcatcgc aactaccacc ccgtgtcctt gacgtgccaa aaataaaatc acaattatgc
    22501 gaataaagtt tgtataaatt tctcctaacc tcgtattcat tatcaccaga tgaataaaaa
    22561 ccaatcctct gaccttcatg atcaaatact gcaacaaaat ctaagccgtt tccatgtctt
    22621 tcaaaaaaga ctagaaattt agaaaatttc caacaaatct gattaaaaag cgtattgagt
    22681 gtcgtactct tacctttgtt ggctgcaccg tatagaataa aaattttagc ttccaccatt
    22741 accaccctcc ccacttatgc tctttcatcg gtccgccgtg ttcgatgtaa tgcacaaact
    22801 catcacggaa atgcttggta aagctgcgaa cagggaaaac ggcggcatcg gcaagggcgc
    22861 agatggtgcg gcctgccatt tggttgccga cggaatccag caaatccaaa tcttccattt
    22921 tgcctttgcc ttctacgatg cggtggacga tgcggtagag ccagcccgta ccttctcggc
    22981 aaggcgtaca ttggccgcaa gactcgtcat agtagaagta gcttaagcgt tcgagggctt
    23041 tgaccatgca cacgtcttca tccatcacga taatcgcgcc ggaacccaac atcgagcctg
    23101 ctttagaaat cgagtcatag tccatattgg tctgcatcat gatatcggca ggcaacacgg
    23161 gcgcggacga accgccggga ataacggcct tgagtttttt gccgccgcgc ataccgcctg
    23221 ccattttcaa gacttcggca aacggtgtgc ccaatggcac ttcatagttg cccggacgct
    23281 cgacatggcc ggaaatacag aacaatttgg taccgcctgc attcggaata cctttatcgg
    23341 caaatgcctg tccgccatca cggacaatga atgggacgga ggaaaaggtt tcagtattat
    23401 taatggtggt cggtttgccg tacaggccga acgaagcagg gaatggcggt ttaaagcgcg
    23461 gctggccttt tttgccttcc agcgattcaa gcaatgcggt ttcttcgccg cagatgtatg
    23521 cgccgtagcc gtggtgggcg aagagttcga attccaaatc cgaacccaaa atatttttac
    23581 ccaaaaagcc tgcggcacgc gcctgctcca aagcggcctc aaagcgttgg tagccttcga
    23641 aaatttcgcc gtgaatatag ttgtaaccgg ctttcgcacc catcgcgtaa ccggcgataa
    23701 tcatgccttc aatcagagca tgggggttga acatgatgat gtcgcggtct ttgaacgtac
    23761 ccggttcgcc ttcgtcggta ttgcagacga cgtatttttc gccggggaag gaacggggca
    23821 taaagctcca tttcaagccg gtcgggaagc ccgcaccgcc gcgcccgcgc aagccggagg
    23881 ttttgacttc gtcaatcaca tcggtttgcg agatgttttc ggacaggatt ttacgcaggg
    23941 cggtatagcc gccgcgtttg acgtattcgt ccaatgtcca gcaatcggga ttggcggtat
    24001 ccacttggtc aaaaatcacg cctgattggt aaatagccat ttttggtgtg cctgtttgtt
    24061 ttcgtatcgg ttgcggccgc tgtttcagac gaccttaaga tgtctttgtg taccggcttg
    24121 taacgtcgtc tgaaataaaa tctagtttat caaatcgata ttttcagacg acctttaatc
    24181 gtttttgttg gatctcgacc acttgcttgt ctgtcgtcat tcccgcgcag gcgagaatcc
    24241 atcctcaatg gtaagcaatg tcttattaaa ttcagaaacc gaatcttacc ggtggattcc
    24301 cgcctgcgcg ggaatgacgg catttcggta tttcagtagg gcggattctt aaatccgaca
    24361 ttttgccttt ttacccactc tgtcggatac aagtatccga cctacgttta aatcgtcgtt
    24421 tcagacgacc tactccaact ccgccagttt cttctcaatc gcttcttcgg tcataaagct
    24481 gcacatgctg tggttgttga ccagcataac gggggcatca ccgcatgcgc ccatgcattc
    24541 gccttcgaca agggtaaact tgccgtcagg ggtagtttcg ccgtagccga taccgagttt
    24601 ttgtttgagg tattcgccgg tagccatacc gccgcgcagg gcgcagggca ggttggtaca
    24661 aacggtcagt ttgtatttgc cgacaggctc aaggtcgtac atattgtaga aagtggcgac
    24721 ttcgtaggct tgtgcaggcg tgatgccgat atagtcggca acaaaagcga tggtttcggg
    24781 ggcgagccag cctttttcgg tttgggcgat acgcaacgca cccatgatgg cggaacggcg
    24841 ttggtcggcg ggatatttcg ccagttctgt gtcgatttgt tttaaggact ctgcggataa
    24901 cattatcggt caacctcccc gaatacgatg tcctgcgtac cgatgatggc aacgacgtcg
    24961 gcgagcatgt ggccttttgc catttcgtcc atgccttgca gatgggcgaa gccgggtgcg
    25021 cggattttca ggcggtaggg tttgtttgcg ccgtctgaaa tgatataaac gccgaactcg
    25081 cctttcgggt gttcgacagc ggtgtaggtc tcgccctcgg gaacgtgcat accctcggta
    25141 aagagtttga aatggtgaat caggtcttcc atacctgttt tcatttcggt acgtttgggc
    25201 ggagcgaatt tgtggtttgt ggtgatgacc ggacccggat tgacgcgcag ccagtcggcg
    25261 cattgtttga tgatgcggac ggattggcgc atttcttcca tgcggcagag gtagcggtcg
    25321 tagcagtcgc cgttgacgcc aacggggatg tcgaaatcca ttttgtcgta cacttcgtaa
    25381 ggctgtgtct tacgcacgtc ccattccacg ccggaaccgc gcaacatcac gccggtaaag
    25441 cctttttgca tggcgcgttc gggggagacg acgccgatgc cgacggtacg ctgtttccaa
    25501 atacggttgt cggtcaggag ggtttcgagt gtgtcgatat ttttcgggaa acgttcgcag
    25561 aagacatcga taaagtcgag catcgtgcct tcgcgggatt cgttgagctg cttcaatact
    25621 ttggcattgc ggaatttgct gccctcgtat ttaggcatga agtcgggcag gtcgcggtaa
    25681 acgccgccgg gacggaagta ggcggcgtgc atacgcgcgc cggaaacggc ttcgtacaag
    25741 tccatcagct cttcacggtc gcggaaggcg taaagaatgg cggtcatcgc gccgatgtcg
    25801 aaggcatgcg aaccgatgcc catcaagtga ttgaggatgc gcgttacttc ggcaaacatc
    25861 acgcggatgt attgggcgcg gataggcaca tcgataccgg caagtttttc taccgccaag
    25921 caatacgcct gctcgttgac catcatggaa acgtagtcca gcctgtccat ataaggcagg
    25981 gcttgcaggt aggttttggt ttccgccagt ttctctgtgc ctcggtgcag gaggccgata
    26041 tgcgggtcgg cacggacgat ttgttcgccg tccagctcca aaatcatacg caatacgccg
    26101 tgcgccgcag ggtgttgcgg gccgaagttg atggtgtagt ttcttaattt attggccacc
    26161 gtagttctcc tcacggacga tacgcggcgt gatctcgcgc ggctcaatgg taacaggttg
    26221 gtaaatcacg cgtttttgct cttcgtcgta acgcatttcc acatagccgg aaatcgggaa
    26281 gtctttgcgg aacggatgtc cgacaaagcc gtaatcggtc aggatgcggc gcaagtccgg
    26341 gtggttgttg aacatgatgc cgtacatatc gaaggcttcg cgttcgtacc aatccgcgct
    26401 gttgtaaata tcgactacgg attcgactac agggaagtcg tcgtctgaaa cccagacgcg
    26461 tacgcggatg cgttgattgt ttttaacgga aagcaactga ctgacgacgg caaagcgttt
    26521 gccctgccat acttcgtttt tgtaagtgct gtaatcgaca ccgcacaagt cgaccagaag
    26581 ctcgaaatgc aactcttcat ggtcgcgcaa tgcggtcatg actgaaatat agtgttcggg
    26641 cagacactcg acggtaatct cgcccaaagc ggaaatgact ttgcctgcct gattgcccaa
    26701 aacgcggctg acggtttcgt ataagttttg aatgcttgcc atatcgtcct ctccttactc
    26761 gtcacgcgcg atggtggaag tgcgcttgat tttttgttgg agctgaatca gaccgtaaat
    26821 cagggcttcc gcagtcggcg gacaacccgg cacataaacg tctaccggca cgacgcggtc
    26881 ggcaccgcgc acaacggaat aagaatagtg ataatagccg ccgccgttgg cacacgagcc
    26941 catagacaat acccagcgcg gctcggcgag ctggtcgtat acgcggcgca gggcgggcgc
    27001 cattttgttg gtcagcgtac ctgccacaat catcaggtcg gcctgacgtg gggacggacg
    27061 gaaaatgatg ccgaagcggt caaggtcata acgcgccata cccgcatgca tcatttccac
    27121 ggcgcagcag gccaagccga aagtaaccgg ccacaatgaa ccggtacgca tatagttcaa
    27181 caccgtatcc gcgctggtgg tgatgaaacc ttttttcaaa acgccttcta ttcccattcc
    27241 agcgcacctt ttttccattc gtaaacaaag cctaccgtca gaacgacgat aaacaccagc
    27301 atagaccaga agccgtacgc acccaaatct ttgaacacaa ctgcccacgg caacatgaac
    27361 gcgacctcca aatcaaacag gataaagagg atggcgacga ggtaatagcg cacgtcgaac
    27421 ttcatcctgg cgttttcaaa ggcctcaaaa ccgcattcgt aaggcgcgtc tttttcggcg
    27481 tagtggcgtt tcgggcccaa aatcgtgccg agcaggataa acagcacgcc ggccgcgagg
    27541 ccgacgagga taaagacaaa gacgggaaag taagcggaca acatggttac acccaaatcc
    27601 gttaacaaaa tttctacaat aatttcgtat tttagcgaat ttcaaaaacc attaaaaggt
    27661 aaatatcggc aaaacgccca aaaaaaccca ataaatacaa tcatgttatg ataacgattc
    27721 ttatttgatt ttataaggtc atataaattt ttacaccgta att
//
LOCUS       DAWKOG010000008        27404 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.008, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000008 DAWKOG010000000
VERSION     DAWKOG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27404
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.008"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(112..2013)
                     /gene="thiC"
                     /locus_tag="WJK66_000283"
     CDS             complement(112..2013)
                     /gene="thiC"
                     /locus_tag="WJK66_000283"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEZ7578055.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGAEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLNE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKSGSNVTQLHYARQGIITPE
                     MEFVAIRERLKLDELSQKSEYAKLLKQHAGQSFGANIPTRPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
     regulatory      complement(2104..2203)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2353..2429
                     /locus_tag="WJK66_000284"
     tRNA            2353..2429
                     /locus_tag="WJK66_000284"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2387..2389,aa:Met,seq:cat)
     gene            2611..3486
                     /locus_tag="WJK66_000285"
     CDS             2611..3486
                     /locus_tag="WJK66_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ7578056.1"
                     /translation="MPTVRFTESVSKHDLDALFEWAKASYGAESCWKTLYLNRLPLGN
                     LSPEWAERVKKDWEAGCSESSDGIFLNADGWSDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGKRSPHKAVDPDK
                     LDNIAAGGVSGGEMPSEAVCRESSEEAGLDKTLFPFIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVACFEKMDIGSLVATMLSGNMMHDAQLVTLDAFCRYGLI
                     DAAHPLSEWLDGIRL"
     gene            3495..4433
                     /locus_tag="WJK66_000286"
     CDS             3495..4433
                     /locus_tag="WJK66_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ7578057.1"
                     /translation="MLELNGLCKRFGGKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIVRPDGGEIQLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAERLAMAALAEVGLENEVHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRGTLRRMTAERIRKGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LVQTPAGVQVARLMGLPNTDDDRHIPQHAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMPHAGEISGNDTVRIHIEDREIVRFR"
     gene            complement(5153..6928)
                     /gene="ptsP"
                     /locus_tag="WJK66_000287"
     CDS             complement(5153..6928)
                     /gene="ptsP"
                     /locus_tag="WJK66_000287"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEZ7578058.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAAH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNVGQLESDIVKVIRCEDE
                     EKSEKLIKQMNSVSVEEEADFKGRK"
     gene            complement(6928..7197)
                     /locus_tag="WJK66_000288"
     CDS             complement(6928..7197)
                     /locus_tag="WJK66_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEZ7578059.1"
                     /translation="MLKQSIEIINKLGLHARASNKFTQTASQFKSEVWVTKNDSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            complement(7266..7703)
                     /locus_tag="WJK66_000289"
     CDS             complement(7266..7703)
                     /locus_tag="WJK66_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEZ7578060.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            complement(7784..8347)
                     /locus_tag="WJK66_000290"
     CDS             complement(7784..8347)
                     /locus_tag="WJK66_000290"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEZ7578061.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            complement(8416..9240)
                     /locus_tag="WJK66_000291"
     CDS             complement(8416..9240)
                     /locus_tag="WJK66_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEZ7578062.1"
                     /translation="MIKKTIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTRKGTPKFATFVRVRTDR"
     gene            9331..9963
                     /locus_tag="WJK66_000292"
     CDS             9331..9963
                     /locus_tag="WJK66_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEZ7578063.1"
                     /translation="MTLRYEILSVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGPHPDDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHIVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFALPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            complement(10169..11953)
                     /gene="rmuC"
                     /locus_tag="WJK66_000293"
     CDS             complement(10169..11953)
                     /gene="rmuC"
                     /locus_tag="WJK66_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEZ7578064.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFADLNAQLAEKAA
                     RCDFVEQAHAEIASELAVLDGKYRHLQDENYALGNRFSAAEKQIAHLQEKEAESVRLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRIETDLEESRQTVRD
                     VQNDLADVGNRFAAAEKQIAHLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     QGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQLAEGRGNLVGRAEKLRLLGVKANKQ
                     LARDLTERANETTALSESLEYVAEDEAV"
     gene            complement(12292..13080)
                     /locus_tag="WJK66_000294"
     CDS             complement(12292..13080)
                     /locus_tag="WJK66_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEZ7578065.1"
                     /translation="MKQAMKNWFAALLLAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            complement(13095..14444)
                     /locus_tag="WJK66_000295"
     CDS             complement(13095..14444)
                     /locus_tag="WJK66_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEZ7578066.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDVLGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            complement(14463..15044)
                     /gene="petA"
                     /locus_tag="WJK66_000296"
     CDS             complement(14463..15044)
                     /gene="petA"
                     /locus_tag="WJK66_000296"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEZ7578067.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            complement(15167..15916)
                     /locus_tag="WJK66_000297"
     CDS             complement(15167..15916)
                     /locus_tag="WJK66_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEZ7578068.1"
                     /translation="MVLRRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            16047..16976
                     /locus_tag="WJK66_000298"
     CDS             16047..16976
                     /locus_tag="WJK66_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ7578069.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAERDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQNGIS
                     FQPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITDDGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELESV"
     gene            complement(17231..17623)
                     /gene="rpsI"
                     /locus_tag="WJK66_000299"
     CDS             complement(17231..17623)
                     /gene="rpsI"
                     /locus_tag="WJK66_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEZ7578070.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(17633..18064)
                     /gene="rplM"
                     /locus_tag="WJK66_000300"
     CDS             complement(17633..18064)
                     /gene="rplM"
                     /locus_tag="WJK66_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEZ7578071.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(18242..18421)
                     /gene="ssrS"
                     /locus_tag="WJK66_000301"
     ncRNA           complement(18242..18421)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="WJK66_000301"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(18427..18732)
                     /locus_tag="WJK66_000302"
     CDS             complement(18427..18732)
                     /locus_tag="WJK66_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEZ7578072.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            complement(18743..19072)
                     /locus_tag="WJK66_000303"
     CDS             complement(18743..19072)
                     /locus_tag="WJK66_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578073.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QRQETQQ"
     gene            complement(19127..20116)
                     /locus_tag="WJK66_000304"
     CDS             complement(19127..20116)
                     /locus_tag="WJK66_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEZ7578074.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(20239..22941)
                     /gene="ppc"
                     /locus_tag="WJK66_000305"
     CDS             complement(20239..22941)
                     /gene="ppc"
                     /locus_tag="WJK66_000305"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEZ7578075.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKTDGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLSFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRNMEHDFQMVYPDVCVPNILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRGELDKLYRELPLSIRRVKVNDDVMALAALSPDEEIARTEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHVDVVAELFQHAGLEDYNRLNEEQKQTALLRELSHQRPLYSP
                     FITYSDHTRRELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKESGLLA
                     VENGKPHSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPEKAAVIFGMIKEEYQRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            22984..23877
                     /locus_tag="WJK66_000306"
     CDS             22984..23877
                     /locus_tag="WJK66_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEZ7578076.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDELLLRYSRHILLDEIGIEGQQKLSDAHILVVGCGGLGAAALPYLAASGVGT
                     LTIADFDTVELHNLQRQVAFDEGDVGKLKTEALAGRLKRINHTVNVRAVNEKLDGCRL
                     TGLVQAADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGCTQAAEALKILLDAGEPSHGRLAVYRALE
                     GGWRYFDLPRNPECPVCGAAR"
     gene            complement(24424..24648)
                     /locus_tag="WJK66_000307"
     CDS             complement(24424..24648)
                     /locus_tag="WJK66_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HEZ7578077.1"
                     /translation="MDAVQELEHRITELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(24649..26037)
                     /locus_tag="WJK66_000308"
     CDS             complement(24649..26037)
                     /locus_tag="WJK66_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HEZ7578078.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGIMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLTGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPIFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(26123..26944)
                     /gene="prmC"
                     /locus_tag="WJK66_000309"
     CDS             complement(26123..26944)
                     /gene="prmC"
                     /locus_tag="WJK66_000309"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ7578079.1"
                     /translation="MTFDKWLGLSKLPKIEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPSVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISTPALETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLLQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            complement(26951..27394)
                     /locus_tag="WJK66_000310"
                     /pseudo
     CDS             complement(26951..27394)
                     /locus_tag="WJK66_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         5990 a         7519 c         7275 g         6620 t
ORIGIN      
        1 ggcttcgtcg ccttgtcctg atttttgtta atccactata ttttcacaag cgaaagaatg
       61 ccgtctgaag ccttttttcc gggtttcaga cggcattttt tgcttgacgt ttcaactgta
      121 aatcttcgcg ccttttttga cgaactcgac cgctttttcc tccatgccct gccgttgggc
      181 tttttgcttg tcggcgtagt cgcgcacttc ctgcgtgatt ttcatcgagc agaatttggg
      241 gccgcacatt gagcagaagt gggcgatttt cgcgccttcg gcaggcaggg tttcgtcgtg
      301 gaagctttcg gcacgttcag ggtcgaggct taagcgaaat tggtcgcgcc aacggaactc
      361 gaaacgcgcc ttgctcaggg cgttgtcacg taattgtgcg cccggccagc ctttggcgag
      421 atcggcggcg tgggcggcga gtttgtaggt gatgatgccg gtgcgcacgt cttctttgtc
      481 gggcagcccc aaatgttctt tgggggtaac gtaacaaagc atcgccgtgc cgtaccagcc
      541 gatattggcc gcgcctatgc ccgaggtgat gtggtcgtag ccgggtgcga tgtcggtaac
      601 gagcgggccg agtgtgtaaa aaggtgcttc aaagcagtgt tgcagctctt cggtcatgtt
      661 ttctttgacg cgttgcagcg gcacatggcc ggggccttcg atcatgactt gtacgtcatg
      721 tttccacgcc ttatcggtca attcgcccaa ggtgtgcagc tcggcgaatt gggattcgtc
      781 gttggcatcg gcaatgcagc cggggcgcag gccgtcgccg aggctgaacg acacatcata
      841 cgctttcatg atttcgcaga tttcgtcgaa atgcgtgtag aggaagtttt cccgatgatg
      901 tgccaaacac catttcgcca taatcgaacc gccgcgcgag acgatgccgg tgaggcggtt
      961 ggcggtcatc ggtacataac gcagcaacac gcccgcgtgt atggtgaaat agtccacgcc
     1021 ttgttccgcc tgctcgatta aggtgtcgcg gaacaaatcc caagtcaaat cttcggcaat
     1081 gccgccggtt ttttccaaag cctgataaat aggcacggtg ccgatgggga cgggcgcgtt
     1141 gcggataatc cattcgcgcg tttcatggat gtgcgcgcct gtggataagt ccataattgt
     1201 gtccgcgccc caacgcagcg accacaccat tttttcgact tcttcggtca ggctggaagt
     1261 gacggcggaa ttgcccaagt tgccgttgat tttgacgcgg aagttgcggc cgataatcat
     1321 cggttcgagt tcgggatggt tgatgttggc ggggataatc gcgcgtccgg cggcgatttc
     1381 ttggcgcacg aattcgggcg tgatttggtc gggacgggtt gggatgttcg cgccgaaact
     1441 ttgccctgcg tgttgtttca agagtttggc gtattccgat ttttgggaca attcgtctaa
     1501 ttttaaacgt tcgcgtatgg cgacaaactc catttcgggc gtgataatgc cttggcgcgc
     1561 gtagtgaagc tgggttacgt tgctaccgct tttcgcgcgg cgcgggcggg tgatttggtt
     1621 gaagcgcaga tgggcggttt tcggatcgtg tgcgcgttcg atgccgtatt cgctggagag
     1681 cttgggcagg atttcggtat cgccgcgttc gttcagccat gcggtgcgga tgtgcggcag
     1741 accttgtttc aggtcgatgt gtgccgctgg atcgccgtac acgccgctgg tgtcatagac
     1801 gggaatcggc gggttggctt ccgcgccttg cgccgtatag gtgtcgtcct gacggatttc
     1861 acgcaaaggc acgcggatgt cgtcgcggct gccttgcaga tacacgcgtt ccgagttcgg
     1921 atatttgaag cggataccga tgtcttcgct caagtcggca agttcgcgcg cttcgttgcc
     1981 ggaagttttg gcggtttttt ttggcgtagt cataaaaaat gctcctgttt tctcgtttag
     2041 aataaagaaa caggagcgtt ttgcgttttc agacggcatt tgaaaaccaa tgctgtctga
     2101 aagacagaat ccgtgaaaac tccccacgca ggtattatcc cgatcgggtg taaagggtat
     2161 ttctcagccg cctgaacatc atgcagcacc cctgtttcaa tgttaaccaa aattaaatca
     2221 cgaacatgaa cttttgtaaa gaaaataata tttcaaatca ggcataaacc gccggacggc
     2281 aaaattttat gatttttcgc ggaagtaatg tttgacaaca taaaaaatct gccgtatagt
     2341 ttcatcttct gacgcgggat ggagcagcat ggtagctcgt cgggctcata acccgaaggt
     2401 cgtaggttcg aatcctgctc ccgcaaccaa atatcaaacc cctcggttca atgccgaggg
     2461 gttttgtttt gcctgtttcc tgccgcctcc gttttttgcc ggattttcct tcctgccgca
     2521 atatcggaac ggcagcccgc cgtctgtttg cggttgcaaa ttcaggcagt ttggctacaa
     2581 tcttccgcat tgtcttcaag aaagccgact atgccgaccg tccgttttac cgaatccgtc
     2641 agcaaacacg accttgatgc cctgttcgag tgggcaaaag caagttacgg tgcagaaagt
     2701 tgctggaaaa cgctgtatct gaaccgtctg cctttgggca atctgtcgcc ggaatgggcg
     2761 gagcgcgtca aaaaagactg ggaggcaggc tgctcggagt cttcagacgg cattttcctg
     2821 aatgcggacg gctggtcaga tatgggcggc cgcttgcagc acctcgcccg cacatggaac
     2881 aaggcggggc tgcttcacgg atggcgcaac gagtgtttcg acctgaccga cggcggcggc
     2941 aaccccttgt tcacgctcga acgcgccgct ttccgtccgt tcggactgct cagccgcgcc
     3001 gtccatctca acggactgac cgaatcggac ggccgatggc atttctggat aggcaagcgc
     3061 agtccgcaca aagcagtcga tcccgacaaa ctcgacaata ttgccgccgg cggtgtttcc
     3121 ggcggcgaaa tgccgtctga agccgtgtgc cgcgaaagca gcgaagaagc cggtttggat
     3181 aaaacgctgt ttccgtttat ccgtccggta tcccggctgc acagcctgcg ccccgtcagc
     3241 cggggtgtgc acaatgaaat cctgtatgta ttcgatgccg tcctacccga aaccttcctg
     3301 cctgaaaatc aggacggcga ggttgcgtgt tttgaaaaaa tggacatcgg cagcttggtt
     3361 gccaccatgt tgtcgggaaa catgatgcac gacgcgcaac tggttacgct ggacgcgttt
     3421 tgccgttacg gtctgattga tgccgcccat ccgctgtccg agtggctgga cggcatacgt
     3481 ttataggatg cgccatgctt gaactgaacg gactctgcaa acgcttcggc ggcaaaacgg
     3541 ttgccgacaa catctgcctg actgtcgggc gcggcaaaat actcgccgta ctggggcggt
     3601 cgggctgcgg aaaatccacc ctgctgaata tgattgcggg catcgtccgg ccggacggcg
     3661 gcgaaattca gctgaacggg gaaaacatta cccgtatgcc gcccgaaaaa cgccgtattt
     3721 cgctgatgtt tcaagattac gcgctgtttc cccatatgag tgcgctggaa aatgcggcat
     3781 tcggtttgaa aatgcaaaaa atgccgaaag ccgaagccga acgccttgcc atggcggcac
     3841 ttgccgaagt cggactggaa aacgaggtgc accgcaagcc tgaaaaactt tccggaggcg
     3901 agaaacaacg gttggcactg gcgcgcgctt tggttgtccg cccttccctg ctgctgttgg
     3961 acgaatcgtt ttccagtttg gacacgcatt tgcgcggcac gctgcgccgc atgactgccg
     4021 aacgcatccg caagggcggc atccctgccg ttttggtaac gcattcgccc gaagaggcct
     4081 gcacggcggc ggacgaaatc gccgtcatgc acgaggggaa aatccttcaa tgcggtacgc
     4141 ccgaaacctt ggttcaaacg cctgccggcg tgcaggtcgc ccgactgatg ggtttgccca
     4201 ataccgacga tgaccgccat attccgcaac atgcggtgcg tttcgaccaa gacggcatgg
     4261 agtgccgcgt attatcccgt acctgtttgc ccgaatcgtt cagcctgtcc gtcctccatc
     4321 cggaacacgg catcctgtgg ctgaacctcg atatgccgca cgccggtgaa atatcgggaa
     4381 acgatacggt acgcatccat atcgaagaca gggaaatcgt ccgcttccgc tgatgcttct
     4441 taaaaacaaa atgccgtctg aaaacctttc agacggcatt ttttaccaaa gcagccatat
     4501 tttttatcag ggctgcaaaa tttatccgaa acaacagaaa ccaccctgcc gtcattcccg
     4561 caaaagcggg aatctagaac ccaacacggc aaaaatttat ccgaaacaac aaaaatcttt
     4621 tcatcgtcat tcccgcaaaa gcgggaatct agaacgtaaa atctaaagaa accgtttttc
     4681 ccgataagtt tccgtgccga cagacctaga ttcccgcctg cgcgggaatg acgggatttt
     4741 aggtttctga tttcggtttt ctgttttaag ggaatgacga gacttgagat ggcggcattt
     4801 atcgggagca acagaaacca ccctgccgtc attcccgcaa aagcgggaat ctagaaccca
     4861 acacggcaaa aatttatccg aagcgacaac aatcttttca tcgtcattcc cgcgcaggcg
     4921 ggaatctaga acgtaaaatc tcaagaaacc gttttacccg ataagtttcc gcaccgacag
     4981 acctagattc ccgcctgcgc gggaatgacg gattttaggt ttctgatttt ggttttctgt
     5041 ttttgaggga atggccgatt ttgggtttct gtttcggttt tctattttgc aagaatggca
     5101 aaatttcaga ttgcgggcat tgttaagtat ttctattttt tacctgccgt atttatttcc
     5161 gccccttgaa gtcggcttct tcctcgacag acacgctgtt catctgtttg atcagctttt
     5221 ccgacttctc ttcgtcttcg cagcggatga ctttcacaat atcgctttcg agctgtccga
     5281 cattgctgtg cagaatgatg tttttgacgg gcaggatgtt gttggggttc atggaaaaac
     5341 ggcgcagccc catacccaat aaaacgcggg taaacgcggt atcgcccgcc atctcgccgc
     5401 atacggatac gtctttgtcc atgcggttgg cggtacggat gacgtgttgc agcattttca
     5461 gcacggcggg atggccgggc tggtagaggt ggctgacgct gtcgtcgccg cgatcgacgg
     5521 acaagatgta ttgaatcagg tcgttggtac cgacggagat gaaatcgacc agtttcaaaa
     5581 tactgccgac ggtcagcgcg gcagacggaa tttcaatcat acagccgatg ccgactttac
     5641 cgaaggcatc gccgcgttcg gcaagctggc gttgcgcggt gtcgaggtgg atgaggcact
     5701 ggcgcacttc ggatacggag gtaatcatcg gccacatcat ccgcacgggg ccgtgggcgg
     5761 cggcgcggag gatggcgcgc atctgggtgc ggaacatgac cggttcggca aggcacaggc
     5821 ggatgccggt catgcccagc gcggggttga ggctgccgtt gggcgtgctg tttttcccga
     5881 accagcgcgg gtttttgtcc acacccaaat cgactgtccg tatcgttacg cttttgcctt
     5941 tcattttttt gacaatcgcg ctgtacactt cgtactgctc gtcttcagac ggcatcgtat
     6001 cgcggttcag gtaaagaaac tcgctgcgga acagcccgat gccgtctgcg ccgaggttgt
     6061 gcagcggttt cacgtcttcg gcggattcta tattgcccac aagctcgatg cagaccccgt
     6121 cagcggtggc ggcggcggtt tttttgagct tgttcaaatc gcgtttgtgg ctgcggtatt
     6181 cgcgggcacg gcggcggtat tcgttcaaca ccgactcatc cggcgcgata atcaacacgc
     6241 cgttgatacc gtccacaatg accgtttcgc cctcggtaat cagtttgcgc gcgttgtgca
     6301 gcccgacgac ggacgggatg tccaagctcc tgcccaaaat cgccgtatgc ccggtggggc
     6361 cgccggcatc ggtaacgaag gcggcaatgc gctgctcttt aaacaaaacc gtgtcggcgg
     6421 gcgaaaggtc gtttgcaatc agaacggttt cgtcaaacag gttgtcggca acttccaact
     6481 cgttgccctg cccgatcagg ttgttgtgga tgcggcggac gacttgcagc atatcctgct
     6541 tgcgttcgcg caaataggca tcgtccatat tgtcgaattg ggcggcgagt ttgtcgctct
     6601 gctgcttcaa tgcccactcg gcgttgattt tttgttccct taaaatatcg acgggttcgc
     6661 gcgacaaggt aacatcggtc aagagcatca ggtgcagcga gatgaacgcg cccaactcgg
     6721 tcggggcgtt ttcgggaatc gcgctgcgga gctgttccaa ctctttgcgc gtggctttga
     6781 cggcggcatc gaaacgttcg gcttcggcat cggtgtccgc ctccgcaaca tcatactgcg
     6841 gcacttcctc cgtaccgcgc gcaatcaggt gggcgcaacc gacggcaatg cctttgcccg
     6901 ccgccacgcc gtgcagcacg atactcatta ttcgccctcg ccgaagtagc cgttgattaa
     6961 gtcggtcagg gcgcgcatcg cttccgcctc gtccgcgccg tccgtctcca gttcgatgac
     7021 cgtacccttg gcggcggcga gcatcatcag ccccataatg cttttgccgt tgacgcggct
     7081 gtcgtttttc gtaacccaga cttcgctttt gaattgggac gcggtttggg tgaacttgtt
     7141 ggacgcgcgg gcgtggagtc cgagtttgtt gatgatttcg atggattgtt tgagcatttc
     7201 gattcccgtg ttatgtatat cggcagcaga cgccgtttaa aatgttttcc tgccctgccg
     7261 cttcttcaga cggcatcgcc gctgcgccgg cacaccaaat cttcgggcgc ggacgtgatg
     7321 gcgaaaatgc cttttaccgc cgcctccctg acgcattcgg taaaggcggc aaggtcttcc
     7381 gccgccggcg aatattggac ggccttaacc atcatcggcg cgttcagccc ggtcaaaatc
     7441 gccgatttgt tttcgcgcac gaggcggcgg gctgcattgc agggggtcgc gccgaaaata
     7501 tcggtcataa ttagcacgcc gtcgttgtcg ggaaactcct gaagcgcggc aatggcgttg
     7561 ttgatgatgt cgtcttggtc ttccgtcggt tgcacgccga gtatgcggac gttttcaggc
     7621 agtccgcccg gaaaaaaatg atgtgccagc ttgcggtagg cttcgcctat ggtttcgtgt
     7681 gtgatgatta aaagccctat catattatgc gtcctgttcc tcgttgtcct accggcgtat
     7741 gggcgcgatg ccgtctgaaa ggccttcaga cggcatcgcg tccttatttt ccgtccaatg
     7801 cgtaaatctc gcccagattg cgccagcagc ccgccgcatc catgccgtaa ccgaaaacat
     7861 aacggttcgg cacatccagt ccgacataat cggctcggat aggcttgggt ttgtcaatca
     7921 gcttgttggc gaacaccgcc gcacggcagc ttgccgcacc catttccaaa agtttggctt
     7981 gaatggcgga catcgtatgc ccttcgtcca aaatatcgtc cagcacgacg acgtgcctgc
     8041 cccggatttg ttccgcatcg ggcatacgct tccagttgaa cgcgccgccc tccagcttgt
     8101 cgccgtaacg ggaaacgtga acataatcaa aatctaaggg aaaacgcaac agcggcagca
     8161 actgccccgt aaacaccacc gcgccgccca tcacgggcag cagcagcgga tatttgccgc
     8221 ccaaatcgcg cgtaatctcg tccgccactt tttgcagtgc ggcacggcat tggccttggt
     8281 cgaacaaaag atcagcgttt tcaagcatcg cctgtgtttc aaggcgtttg gtttctaaat
     8341 cggtcatatg tcggaatcgg tcggtaaaag gaaaattata aaccaaagta tcggatgccg
     8401 tctgaactgt cctgctcagc ggtcggtacg cacgcgcaca aatgtggcaa atttcggcgt
     8461 gcctttccgc gtaaagccac ggtaacggta ggtaatcagc gtgccgattt tgggcgggtt
     8521 gtcgcggtct ttgtccttga aaccgctgcc gatgcggaat tcgccgtgtc ggtttttgca
     8581 gccgaccgcg cccagccgtc cggcgtttcg ccctttgccc tcatagtgcc gcgttaccgt
     8641 gcattcgtcg tcgtattggc ttttcagctt caataattgg ctgctcctgc cgccgctata
     8701 acgggattcg ggctggcgca gcatcacgcc ttcgccgccc tgcgcttcga tttgttttaa
     8761 aaagtccatc gcgtgctgcc ggtcgcgcac tttgatttgc gggatgatgg taatcggcgc
     8821 gttcggatgc gttttcagcc actgcgttgc gactgccaaa cgttggtaga gattgccctg
     8881 cgccttgggt acgtcgaaaa cgtgcagacg gatgccgcgc cagtctgaag aaacagaacg
     8941 cacggtagcg gaaatctgct cgaactgacc gcgtccgcta tacaattcgc cgtccaaagg
     9001 ataaggcgga aactgcgcgg taaagccttt ggggggcgta aacgcgtagc cctgacggct
     9061 tatcaggtgc tttccgtccc aataggcgcg cacgccgtcg agtttctcgc tcattgccca
     9121 gccggcaata tcctgccctt tgtattcctg cgccagcatc aaatccgccg cgcctgccga
     9181 tgcagggatg aaaaccgccg taaaaatcgg tatgatgccg ccgattgtct tcttaatcat
     9241 ctgattcccc aaatatcaaa acaggcggca aaccgccata aaacaaacgg caaacccgat
     9301 gccgtctgaa aaaccgttta ggaacacgcc atgaccctac gttacgaaat cctctccgtt
     9361 acccccttcc gccaaaactg caccctgatt tgggacgacg aaagcggcga agccgtcctg
     9421 accgatgtcg gcggcgacgt gccgttcctg ctgcaagcgt tggcaaaccg caaacttacg
     9481 ctcacggcaa tctggctgac gcacggccat ctcgatcacg cgggcggcgt ggtcgaaatg
     9541 ttgaaaacgc ataaagtccc tgtcctcggg ccgcatccgg acgatgaatt cctgctccaa
     9601 tcgctgccgc aaaccaccgc gcaatacgga tttcctgtct cgcccgcctt tgcgccgaac
     9661 cgttggctcg aagaaggcga aacgctcacg gtcggacgct atgcctttca agtgctgcat
     9721 attccgggcc atacgccggg acatatcgtc ttttattgtg ccgaggcgga attgctgatt
     9781 gcgggcgacg tgctgtttta cgaaaccata ggcagaaccg attttccgcg cggcaaccac
     9841 gccgacttaa tcaataatat ccgcaacaaa ttattcgccc tccccgaaac cgtgcaagtt
     9901 gtcgccggac acgggcgtat gacttccatc ggacacgaaa aacggcacaa tccgtttttc
     9961 taaccgactt cactacggtc ttcagacggc gtcatctgca ctgatgccgt ctgaaacgca
    10021 aaaggctcag acaaccgccg ccttgccggt gtacctcgcc ggacaaggct ttggtaagta
    10081 cttcaaacaa accccaaatc agaaccggta cagttacgcc gcgctttcgg cattcccgcc
    10141 cctgctgaaa caagcttttc cgcacaagtc agactgcttc atcttctgcc acatattcca
    10201 aagattccga caacgccgtt gtttcattgg cacgttcggt caaatcgcgt gcaagctgct
    10261 tgtttgcctt cacgcccaac agacgcagtt tctcggcgcg tccgaccaga ttcccgcgcc
    10321 cctcggcaag ttgcttaaat gccgtctgaa agctgttttg cgcctgatcg atgcctttgc
    10381 cgacgctttc gagcgtctgt acaaagccga caaatttgtc gtacagcttg ccgccttcgt
    10441 ccgcaatcgc cagcgcgttt tggttttgct gctcgttgcg ccaaatattc gccaccgtcc
    10501 gcaaagtcgc cagcagcgta ctggggccga ccagcataat ccgtttgtcg aaacactctt
    10561 ggaacaagcc agcgtcattc tgcaacgcca acaggtaggc cggttcgaca gggataaaca
    10621 taaagacgaa atccaatgtg ttcacacctt ccaaatcggt gtaatccttc agcgacaagc
    10681 ctttcatatg cgcgcgtatg ctggcgacat gcgccgccaa ctcgcgtgcc gccgtatccg
    10741 catccgccgc ctgcgtgtag cgcacataag ctgtcagcga gaccttggag tcaatcacaa
    10801 tctgcttgtt gtcgggcagg ttgaccaaaa cgtcgggctg gaggcggcgc gtgccgccgt
    10861 cttcctcttt tcggacggat gctgcctgaa ccacatattc ccgccctttc tgaaggccgg
    10921 aattttccaa aaccgtttcc agaatcatct cgccccaatt gccctgaacc ttattctgcg
    10981 taccggtcag cgcgttggtc agggcctttg cctcgctgtg cagctgcgca ttcaatcctt
    11041 gaagccgttt caattcgttt tccaacgtca gccgctcgcg cgattcttta tcataggttt
    11101 gcttgaccaa ctcgccgaaa ccttggatgc gttcgtttag cgggttcaaa acctgatgga
    11161 gctgctcccg gttctgctcg gtaaaacggc ggcttttttc ttccaaaatc gtgttggcaa
    11221 gattttgaaa ctgatcgctc aaacttttgc gcgcctcgcc cagcaaggac agcttctctt
    11281 cagaagcaag gcgttcctgt tcgatttgcg ttgccaaacg ttcgttttca accgccaaac
    11341 cctgtgcctt ttcctgcaac tcggtatgcg actgcctcaa ccgctccgct tccgcctctt
    11401 tttcctgcaa atgggcaatc tgtttttcgg ctgcggcaaa acggttgccg acatcggcaa
    11461 ggtcgttttg cacgtcgcgg acagtttggc ggctttcttc caaatcggtt tcgattcttt
    11521 ggcggatttg gcgttccaag gcatattggt cggcaaacgc cttccgttcc tgtccgagct
    11581 gcgttgccaa acgttcgttt tcaaccgcca aaccctgtgc cttttcctgc aactcgatat
    11641 acgactgctt cagcctaacc gattccgcct ctttttcctg caaatgggca atctgctttt
    11701 cggctgcgga aaaacggttg cccaaagcat aattttcgtc ctgcaaatgt cggtatttcc
    11761 cgtccaaaac ggccaattcc gatgcaattt ctgcgtgtgc ctgttcgaca aaatcacatc
    11821 ttgccgcctt ttccgccagt tgcgcgttca aatcggcaaa ctcgccctga aacctgccct
    11881 tcatcagcaa ccatgtaaac aacacgcccg acaccaacgc cgccaaaggc agcaaaacag
    11941 tcatcagttc catcaatcat cctaatattc aaacattttc acaccggaca aaaccgccgc
    12001 ttattccgat tctacctgtt tgttcgacat agctccaaaa aatatagcgg attggcttta
    12061 aacctgttcg acatcgcctt accatgctgc ttgcggtttc agaccttttc ctaattcaat
    12121 atcaatctgc cacaaaccct gattaagttc ccgatgtctg acatttttag aatgatgccg
    12181 tctgaaatgt tgcagctatg ttcagacggc atacggattc aggcttttca aacggcaggc
    12241 aaaatgaaaa aagggcaaac cctaaaggat ttgccctttt gttccaaacg cttagtgtac
    12301 gtctttccaa tattcttttt tcaggaaata cgccaaaggc agcataaccg caaataggaa
    12361 aatcatcacg acatagccta tacgtttgcg ttgcagttgt gcaggttcgc ccatgtacac
    12421 aaggtaattg accaaatcgc gtacatatgc gtcgtactct ttttggatca ctttgccgtt
    12481 aggcagacgg cggctgtgca aaccggtaga ttcccaatac agcttaggct tcatctcgcc
    12541 gtgttcgtct tttaccataa ccggctgacc tttggcatcc aactcaacgg cttgaacgcc
    12601 ttgctgttcc cacaacgggt ggggcatacc gactttatcg aatacagtat tgttccagcc
    12661 gctcggacgg gtcggatctt tatagaagcc gcgcatataa gcgtaaaggt agtctgcacc
    12721 tttggaacgc gcaatcaatg tcaaatcggg cggagcagca ccaaaccatt ttgccgcatc
    12781 tttcgggttc atcgccgaat gcatgacatc gccgacatta tcggtggtaa acatcaggtt
    12841 tttcttgatt tcttcgtcag tcaaaccgat gtctttcaaa cggttgaaac gcataccgct
    12901 tgccgagtgg caggacaggc aatagttggt aaagatttgt gcgccgcgct gcaggctgac
    12961 ttggtcgcgc aaatcgatat cgactttctc atagtgtgca tgaccgcccg aagcaaatgc
    13021 cgcacccatg ggtactgcca acagcaaggc agcaaaccaa tttttcatag cttgtttcat
    13081 tttgactgcc ctcatcagat gttggttgca aacaggtaag cacccacggc ggtaatgccg
    13141 acgtaaacaa agaacatgat tttttgtttg gcggtgctca tggttacgcg ttcgggaacc
    13201 ggtttgtttt tatccagctt ggtatagaac ggcataccca ggaagaatgc gaaatagacg
    13261 aaggacagga tgcgcgcgac caaagtacgc gtatcggttg ccaccatcgc gcccaaaata
    13321 cccaaaccga taaaggaaat gatgaacaag accaatgcgg ttttgaaaat cgggccgcgg
    13381 taacggacgg atttcacctc gcccctatcc agccaaggca gcagggcaat caacacgacc
    13441 gccgcgccca taccgattac accccaaacc tgcgttccca agaacgaggg aatcgcacgc
    13501 aaaatggcgt agaacggtgt gaaataccat accggcgcaa tgtgcggagg tgttttcagc
    13561 gcatttgccg catcgaagtt cggcgcttcg aggaagtagc cgccgccttc cggtgcgaag
    13621 aacaatacgg cacagaagac aatcaggaat acaaccacac ccaagacgtc tttaacggta
    13681 tagtaaggat ggaatgggat accgtcgcgc gggataccgt tttcgtcttt gtttttcttg
    13741 atttcaacgc cgtcagggtt gttagaaccc acttcgtgca gggcgatgat gtgcgccaca
    13801 accaagccga gcaataccag aggtaccgcg ataacgtgca gggcaaagaa gcggttcagg
    13861 gtaacgtcgg aaacgttgaa gtcgccgcga atccaagtgg acaaatccgg accgataaca
    13921 gggatggcgg agaacaggtt aataattacc tgcgcacccc aaaaggacat ctgaccccaa
    13981 ggcagcaggt agcccataaa ggcttccgcc atcaatgcca agaaaatcag agaaccgaaa
    14041 atccacacca actcacgggg ttttttgtac gaaccgtaaa tcaaaccacg gaacatgtgc
    14101 agataaacaa caatgaagaa gaaagatgcg ccggtggaat gcatgtagcg gataatccaa
    14161 ccgccggaca cgtcgcgcat gatgtactcg actgcagtaa aggcagcagg cagatggtag
    14221 gcgttcaggt tgccgtcggg tttgtagttc atggtcagga aaatgccgct gacgatttga
    14281 atcacgagga caagcagggc caaagagccg aagtagtacc aaaaattaaa gtttttcggt
    14341 gcgtagtatt gagccaaatg ctcattccac attttagaca gggggaaacg agcgtccatc
    14401 cagcctaaca atgcttttgc tttgctattg gtttggtttg ccataattat cgttccttat
    14461 tcttagtctt cgcccaccaa gatagttgtg tcgctcaagt atttatatgg cgggacaacc
    14521 aggttggtcg gggcaggaac acctttatat acgcggccgg ccaagtcgaa tttcgaaccg
    14581 tggcaggggc agaagaagcc gcctttccag tctgcaccca aatcggcagg ggcaatgtcg
    14641 ggacggaagg tgggcgagca gcccaaatgg gtgcagatac cgatggcgac aaggatgttc
    14701 ggcttaatcg aacgggtctc gtttttagca tactccggct gctgttccgc atcggaattg
    14761 ggatcggtaa gttcgccgtt caggcctttc aggtctttaa gctgctgatc tgtacggttg
    14821 agcacccaaa tcggtttgcc ttgccactcg gcggtcagca actgacccgc ttcgatttta
    14881 ctgacatcca cctcgacggc agcaccggcg gccttggctt tttccgaagg gaaaaaactg
    14941 gccacaaacg gcgttgccac acccaatgct gccactccgc ccgcgccgca ggtcgcgagt
    15001 gtcaggaaac ggcggcggcc gttgttgatt tcttgattat ccattattca gtcgtcctaa
    15061 tattttggga ataccgagcc attaaacgtt gcaattttac ccagtttgca gtgatactca
    15121 aagcattatt taaaataagg taaagtttta tgatatttct caagactcaa gccggattgt
    15181 tttcgtcaaa atggcacact tccaacccga aaacctctgc cgccgattct gccagcgcgc
    15241 gtacgccgta acgttccgtc gcgtgatgcc ctgccgaaat gaaagccgta cccgtttcat
    15301 tggcaaggtg gtattgagct tcagagattt cccccgtcaa atacagatcg acaccttcgt
    15361 ctattgccgt ctgaaaaaac ccctgcgccc cgccgctaca ccatgcaacc cgtcggattt
    15421 cgcgttcggg tttgccgatg acgacaggct tacgttgcaa aactgtttca atatgcgccg
    15481 ccaatgcgcc gagtgtcttg gcttgtttca ggctgcccga gttgagcagg ttttgttcgc
    15541 cgaaccgttt ttctgtcgca aaacccaatc tgtcggcgag ttgggcattg ttgcccagtg
    15601 tgggatgtgc atccaggggc agatggtagc ctgccatatt gatgtcgtgc cgtaacagtg
    15661 cggcaatccg ttcttttttc caaccagtaa cggtcggcaa ctcgtttttc cagaacatac
    15721 cgtgatgtac caaaagcaaa tctgccttct gctccacagc aaaatcaatc gctgccctgc
    15781 ttgccgttac cgacgtaacg attttcccga tatattccct cccttcaacc tgcaaaccgt
    15841 taggggcgta atctttaaac aacgctgtct gcaatgtttc attacaccaa gtcagaaaat
    15901 ccctgcgcaa taccatcttt tttcctaatc gctttaaaca agcgggcatt ctaatcgcaa
    15961 aatgtccgga attcacattt ttccgatttg cacccgcata tgaattattt taatatgcgc
    16021 cggttcaata tgccgtctga agccccatgg attccattat cgaattgcgc cacctcaaaa
    16081 ccctgctggc acttgaagaa accggcagcg tctcccttgc cgccaaacgg gttttcctta
    16141 cccaatccgc cctttcccac cagatccgta tgctcgaaaa ccactacggc acgccgctgt
    16201 tcgaacgcaa atccacgccc ttgcgcttta ccccggtggg cgaaaggctg ctgcgcctcg
    16261 cccacgaact gatacctcaa gttgctgttg cagaacgaga tttggcgcga atcacagaag
    16321 gggaggcggg agagctgcgg attgccgtcg aatgccatac ctgtttcgac tggctgatgc
    16381 ccgccatggg cgaattccgc ccgatgtggc cccaagtcga attggatatc gtatcgggat
    16441 tccaagcgga tcccgtcgga ctgctgctgc aacaccgtgc cgaccttgcc attgtttccg
    16501 aagcggaaaa acaaaacggt atttctttcc aaccgctgtt tgcctacgaa atggtcggca
    16561 tttgcgcacc agaccatccg cttgccgcca aaaacgtttg gacggcggaa gactttatcg
    16621 gggaaaccct gattacttat cccgttcccg acgagatgct ggatttgccc aaaaaaatcc
    16681 tgattccgaa aaacatcaac ccgccgcgcc gacacagcga actgaccatc gccattatcc
    16741 aactggttgc cagcagacgt ggcattgccg cccttcccta ttggacagtc atgccctacc
    16801 ttgaaaaagg ctatgtcgtt caccggcaga ttaccgacga cggactgcaa agcaaactgt
    16861 atgctgccat ccgcactgaa gatacggaca agagctatct gaacaacttt tgccaaatca
    16921 tacgtgaacg cggttttgca gatttgcccg gactgagcga actggaatcg gtctgacccc
    16981 ttattcaacc atacccggca gtttttctat tttttcatgt atagtggatt aacaaaaacc
    17041 agtacagcgt tgcctcgcct tgccatacta tttgtactgt ctgcggcttc gtcgccttgt
    17101 cctgattttt gttaatccac tatactgttt ttgatttttg cccaatctgt aatctttaga
    17161 ttgccaatgg ggaaccgtct actacaaata aaaaaccctg cgataagcag ggttttttga
    17221 atttccaaca ttaacgtttg gagaattgtt ttgcacggcg tgctttgcgc agacccggtt
    17281 ttttacgttc gacttcgcgg gcatcgcggg taacaaaacc agcttgagac aaggcgggtt
    17341 tcaacgcggc atcgaagtcg atcagggcac gggtaatgcc gtggcggatt gcgccggact
    17401 ggccggtttc gccgccgcca acaacattga ctttgatgtc gaaagattcg gcgttttcag
    17461 tcagaaccaa gggttggcga acaaccattc ggctggtttc ccgtgcgaag aattcgtcaa
    17521 cgggacgacc gtttacgatg atttgacctg tacctttaat caggaataca cgagccactg
    17581 aacttttgcg gcggcctgtg ccgtagtagt atttaccgtt catgtcgcgt ccttatttca
    17641 gttccaaaac tttgggttgt tgcgcagcat gggcgtgttc cgcacccgca tacactttca
    17701 gttttttaat catggcgtaa cccagaggac ctttgggcag catacctttt acagcttgtt
    17761 ccaaagcgcg gcccgggaat tgctcttgca tttcgcggaa ggtgcgttcg tagataccgc
    17821 ctgggaaacc ggaatggcgg aagtattttt tatcttcgaa tttggcaccg gttacacgca
    17881 gtttgtccgc attgataaca atgatgtaat cgccggtatc gacgtggggg gtgtattcag
    17941 gtttgtgttt gccacgcaga cggctggcga cttcggccgc aacgcgaccc aagactttgt
    18001 cttgggcatc gatgacgaac cattcgcgct tcacctcgtg gggtttcgct gaaaaggttt
    18061 tcatagtgga aatccagata gatatagaaa gttgtaaatt ttaaagacag gattcgattt
    18121 tgtcaatcgc attaccgcgt tacggaagga ttttccggat ttcggcagac tgcatactgc
    18181 tttttcgggc ggggcggcgg ccaatgtgaa aaaccgcatc gttgcgatgc ggttttgaat
    18241 gggaatcccc gcgagagccg tttcggccga atccgcttga accttgctga caaggcggct
    18301 gcctcgggta gtttcgggtg cgtccgcaaa aggacgctcg cgcccactac tgctcccggc
    18361 aaccttaagc gaacttattg gttcaaagga atatatgcct tcgcggacac cgcagggaaa
    18421 aaggggttat tcctgcgcca agcgggatag tgctttttgg caggcgttgt ccatatcggc
    18481 tattttacgc gcaaaatcgc cgattgccaa atcgccgccg ttcagggagg ttttcaacag
    18541 gtcgtggacg acgttgagcg cggccataat gacgattttt tcgctgtccg cgacgcgtcc
    18601 gccttcgcgg atggcttcgg ctttgccgtt gagcattccg actgcctgca acagtgtgtc
    18661 tttttcttct gccggcgtgt tgacggtcag ccgggcgtgc atgacttcga tgtggacttg
    18721 ttcgatgttc atcctttaat ccttattgct gcgtttcctg ccgttggggg aggcgcgctg
    18781 ccagtgcgct gattttttcc ctgctctgtt cgagcaggct gcggtatcgt gtattttctt
    18841 ctgtcaggct gtcaattttg ttttgcaggt cttctttgag tttgccgact tggacgagca
    18901 gggcttcgct aagttcgtcg acggcggttt cgtgttcgag tttttgccgc tcgtgcgccc
    18961 gtttgagttc ggcgacggtt tctttgaggc ggcggttttc gctgacgagg gtttcgaatt
    19021 tttgtaccag cgtataaacg ctgctttcga gtttttcgat attttgtttc ataaccttac
    19081 ctgtccgtat gccgtctgaa ggcttcagac ggcatctgtc tgttgtttat tcaaaacgcg
    19141 cgctacgttc catcagcctt tcgacgacct gctgaggggt catttcctta cggatgagtt
    19201 gcagcagggt ttgggtaatc ggcatatcga tttggtactt acaggcagta ttgaagactt
    19261 cttctatcgt gctgacccct tcggaaacgt gtccgatttc gaccagcacc tgatgcagtt
    19321 ccttgccttc tgccaaaccc aagccgacgc ggcggttgcg tgagagtgcg ccagtgcagg
    19381 tcaagatgag gtcgccgatg cctgccagcc ccatcatggt tttgggctgt gcgcccattg
    19441 cggaggcaag gcgggtaatt tcagctaatc cgcgcgtaac cagtgcggca cgggcgttaa
    19501 gcccgtactc taggccatcg gacaatccgg tggcaatcgc cataacattt tttaccgcgc
    19561 cgccaaccgc cacgccgata acgtcggtac tgccgtaaag cctcatgaca gtcgtgttga
    19621 gctgcggtac gagttcttca atccactctt ggttttcgga ggcgaggacg acggcacagg
    19681 gcagttgttt ggcgagttcc tgtgcaaaac tcgggccgga gagtacgccg attttcttat
    19741 tgtcgggcaa tacttctttc aatacttgaa aggtcagcag ccctgtatcc tgctcgaatc
    19801 ctttgcaggc ggcgaggacg gggaggtgtc ccgcgccgta ctgtttgagc agctctgcgc
    19861 tgcttctcaa tccggcaacg gaggttacga taaggacaag tccgctgtct ttgagcgcgt
    19921 ctgccaaatc cgcacacact tccaaggttt cgggaaagga aaagccgggc agtccgcgtt
    19981 tgttttcacg cgcttcctgc atttggcgga cttggtctgc gttgcgcgtc cacagggata
    20041 cgcggttgcc gtgttgggaa aagtgcaggg cgagcgccgt accccacgaa cctgcgccga
    20101 taacggtaat tttcattggt cgtctttcaa catatcactg ccgttcactt taaaacaatc
    20161 ggtgtttctc tgcaagtgcg gtcagggaaa tgccgtctga aaggtgttca gacggcattt
    20221 tgccccgatg cggcacaatc agcctgtatt gcgcaaacct tgcgccacgc cgttgatggt
    20281 caggtgaacc atcagcagcg cgtgcggatt gtcgggttct ttacgcaggc gtttgagcat
    20341 ggcgacctgc aggccgttga gcgcgttcag gtagggaatc ctcaaggcaa gcgaacgggc
    20401 gaggctgcgg ttgtcgcgta aaagttcttc ggtttgcagc aggtcgagca gggctttgcg
    20461 gctgcgttgg tattcttcct taatcatgcc gaagatgacg gcggcttttt ccggcgattc
    20521 gctcaagccg gcatagtttt ccgcgagggt gatgtcggtt ttcgccatca cttgctccat
    20581 attggaaagc atggcttgga agaacgggtt gctttgggcg tgttcgcgca gggcggcgag
    20641 cgtttcgggt ttgtcttcgc acaaggtttc caccgcgctg ccgaaaccgt accaagcagg
    20701 cagcatgagg cggttttgca tccaggaaaa tacccacgga atcgcgcgca agtcttgaat
    20761 ccgcgctaag gtcttgcggc tggcaggacg gctgccgagg ttgagggtgg cgatttcctg
    20821 aatcgggctg gtttgcagga agtagtcgat gaagtcgggg tgggtaatca gttcgcggta
    20881 gtatttgaac gatacgtccg acaatgcctg catcagtttg gcatcagggt cttttttatc
    20941 cggcaggatg ctggcttcca aagtcgcggc aaccaaggtt tccaagttgc gttgggcatt
    21001 gccggggtcg gcgtatttgg cggtaatgac ttcgccttgt tcggtaatgc ggatttgtcc
    21061 cgccacgctg cccgccggtt gggcgagaat ggcttggtaa gacgggccgc cgccgcgtcc
    21121 gacgctgccg ccgcgaccgt ggaacaggcg catgcggaca tcgtattttt tgaagagttc
    21181 gaccaagccc aattccgcct gatacagaca ccatgagctg gtaacatagc cgccgtcttt
    21241 gttggagtcg gagtagccga gcatgatttc ttggatgttt ccgcggcttt cgagcagtgc
    21301 gtcgtaccaa tcgaggcgga acatggtttc catgaccgga caggcgtttt cgagtgcttc
    21361 gatggtttca aacagcggca cgatattgat gcggctgtgc ggtttgccgt tttccaccgc
    21421 caacaggccg ctttctttca gcagcaatgc caaggcgagc aggtcgctgg gttgttcgca
    21481 gttggaaata atgctttgtg taacggcatc ttcgccgaat tcgtctttga ttttgcgcgc
    21541 ttcgttgaaa atcgccagtt cgcggcgggt atggtcgctg tatgtgataa acgggctgta
    21601 cagcggacgt tgatggctca attcgcgcaa cagggcggtt tgtttttgct cttcgttcag
    21661 gcggttgtag tcttccaagc ctgcgtgttg gaaaagctcg gcaaccacat cgacgtgttt
    21721 gcctgcgtgt tggcgcaggt cgagcggcat catgtggaag ccgaacacgg acacggagcg
    21781 gatgaggtct gccaaacggc cttcagcaag caggcggctg ccgttgtcga taagggaacg
    21841 ttgcaatttt ttcaaatcat cgagaaattc ttgtgccgaa gcatacggct cgagaaagcc
    21901 gaatttgcag cccatgccca aaccgagcgc gcgcgctttg cccatagcgc gcgccatgat
    21961 gtaagcaatg gcgcggcggt agggttcttc ggtgcgagcg atttcttcgt caggcgagag
    22021 ggctgccagt gccattacgt cgtcgttgac tttgacgcgg cggatggaga gcggcagttc
    22081 gcggtaaagt ttgtcgagtt cgccacggta aaaacggaac acggcatcgg cgtggcggcg
    22141 gaaggcaaaa cgcaaggttt cggcagaaac gaacggattg ccgtcgcggt cgccgccaat
    22201 ccagccaccg attttgagga tattcggaac gcagacatcg ggatagacca tctgaaagtc
    22261 gtgttccata ttgcggtaga gcttgggcag ggcttcgaaa aagctcatcg ggaagatgga
    22321 cacgccgttg ttgatttcgt cgttgacgct gagtttgtgg cggcgcgttt cgctggtctg
    22381 ccacaagccc agcaggatag tgtcgatttc gcggcgcagc cgtgccagcg cgtcggcatt
    22441 ggtgcagcgt tcgcgttgcg gcaacagtgc gcggatgcgg cggttgaagc ttaagacggt
    22501 ttggcgttgc acttcggtcg ggtgcgcggt caaaacggcg gtaacggacg tattgtccaa
    22561 ctgccgctgc accgatttgc cgtcggtttt ccccgctttg agcctgcgga cggtttccgt
    22621 caggctgcct tccgcgccgc cgcgtccggc ttcttcgtgg atttggcggc ggcgttcgtg
    22681 gtgcacgtct tcggcgatgt tcaaaatctg ggcgaacagg ccgcaggcca aggttaaatc
    22741 gtgggtttgt tgttcgtcca attgcggcaa tactttttca atcaatgccg cgctgtcgtc
    22801 ggaagtggac aagagtttga ccgtttcgac aaccaacggc gaggcttctt cgtgcaggag
    22861 gttgaacagg gattgtttca gaaattccgc gtccgccgcc aaagccgcgt ccttcggatt
    22921 gttcagaata tgcagttgca tgatttttct ctcattgccg taaatactgt aaatgtacct
    22981 caaatgccgc atccgtgcca aaccgttcac actttaaccg cccgtgtccc gaaatgccgt
    23041 ctgaagttga acgccgcccg acggcagcgt tacaatcgcc cgcaactgtt ttttccgaac
    23101 atcatcatga ccacgaccga acacgacaac gacgacgaac tcctgctgcg gtacagccgc
    23161 cacatcctct tggacgaaat cggcatcgaa gggcagcaaa agctttccga cgcgcatatt
    23221 ttggtcgtcg gatgcggcgg tttgggtgcc gccgcactgc cctaccttgc cgcttcgggt
    23281 gtcggcacgc tgaccatagc cgatttcgac acggtcgaac tgcacaacct gcaacgccaa
    23341 gtcgcatttg acgagggcga tgtcggcaaa ctcaaaaccg aagccttggc aggccgcctg
    23401 aaacgcatca accataccgt caacgtccgc gccgtcaacg aaaaactcga cggctgccgc
    23461 ctgaccggtt tggttcaagc cgccgacatc gttttagact gttgcgacaa ctacgccacg
    23521 cggcaagccg tcaaccgtgc ctgcgtgcaa acgaaaacac cgctggtttc aggggcggcg
    23581 gtacgctttg aagggcaact tgccgtgtac cgacccgact tgcccgactc gccatgttac
    23641 gcctgcctgt tcgacggcgg atcggcttca gacggcatct gttccctttt cggcgtattc
    23701 tcgccgctgg tcggcatcat cggctgcacg caggcagcgg aggcactgaa aatcctgctg
    23761 gatgcaggcg aaccgtcgca cggcaggctg gcggtttacc gtgctttgga agggggctgg
    23821 cgatatttcg acctgccgcg caaccctgaa tgcccggttt gtggcgcggc acgataaacc
    23881 ctgccgccgt ttcagacggc ataaaaacgg attcggcgga acggttttaa aaatcggcgg
    23941 tgttttgaca tagtgtcgat aagcatactt atcttcaata cactcaacag gataattgtt
    24001 tgcctaagca aaaaaataat cgccttttcc ctgacgggcg ggcgaaatcc acggattgca
    24061 aaaacacaac ctgaaaattt acatttcttt gcaaaaaaaa aaaaaaaaaa ataaacttac
    24121 cttataatta cggatgtctt agcaatatct gcttcgcaga ctcattgagt aagatatttt
    24181 ccccgtaatg tgtttggccg tctgtcccct ttgggttcgg atggcttttt tttttgactg
    24241 tgtttgaata cccggttgat ttcgtctgtt tgcaggggga aagccgctta tttccgttcg
    24301 ggtggaaaac ggttccatcg gataaaaggc attttgtccg actgattaaa gttacatctt
    24361 agagccgcat cggcggaata ttcaaacaca gcttttttaa ggaaatccgg atacggcggc
    24421 gcatcaataa tgcggcggaa tctcgtcgcg cagggaatac ggctcttgcg cgtcgggatt
    24481 cctgtcctgc attttttgat acagcagcct caactgagcc tgttgcaaat ccagcgtctg
    24541 ccgcaattcc gccaccatcg cgttcaggcc ggcgattacg tcctcctgaa gcgcggattg
    24601 gatttccagc tctgtgatgc ggtgttccaa ctcttgaacc gcgtccattt acagcaccat
    24661 cgcggcaatc cagccggcaa tcagcagcgg gatgttgtag tggatgaaga tcgggataac
    24721 ggaatcgcgg atgtggtcgt gctgcccgtc ggcgttcagc cccatcgtcg gacccagcgt
    24781 ggaatcggac gcaggcgaac cggcatcgcc caacgccccc gccgtgccga caatggcgac
    24841 ggtggcaagc ggcgaaaaac ccaaaccgac acacaaaggc acataaatcg cggcaataat
    24901 cggcaaagtg gaaaaggacg aaccgatgcc catcgttacc aaaagtccca ccaccagcat
    24961 cgccaacgcc gccatacctt tgctgttgcc gaatatcgcc atactgcttt ccaccagcgg
    25021 ctggatatgc ccggtcgcat tcatcacggc ggcaaaaccc tgcgcggcaa tcataatgaa
    25081 gccgaccatc gccatcatct tgataccttc gccgaatacg tcgtttgcct tgtcgcggtt
    25141 gatgaccccc aacatcataa atacggcgaa accgagcatc gcgcccaaca ccagcgagtc
    25201 ttcatacatc aactggatgg caaaacatac ggcaatggcg gcggcggcgg ccaggctgcg
    25261 gtaggcggac ggctgcggac ggtttgccgc atcggcgttg cccgccgtat cggcattgtt
    25321 gctttggtac aggcgcggtt tgcggtaatg gacaaacgcc agcaggagtc cggtcagcat
    25381 tcccaacgcg ggaatcgcca ttgccgccat tacgttgatg tttttcacat caagctgcgg
    25441 cgcggcggaa tggatgttgc ccaacaggat ttcgttcaaa aaaatcgcgc cgaagccgta
    25501 aggcaggaac atataggtcg taaccagccc gaaagtaatg acgcacgcaa tcagacggcg
    25561 gtcgattttc aggcggttga acaccaaaag cagcggcgga acaatcatcg ggataaaggc
    25621 gatgtggata ggtatgatgt tctgactcat aatgcccatc acaaggatga tggaaagcag
    25681 cagccatttg accgcgccct cgcccgaacg cacgctgtcg ggcataccgc cccggttcag
    25741 cttgcggacg accgcgccgg caagctgctg cggcaggccg gaatgggtaa tcgccattgc
    25801 aaacgcgccg agcatcgcat aagaaagcgc aatcttcgca ccgccttcca aacctttgtt
    25861 gaacacgggg ataatccccg cctgactgac ctgtcccgcc gcatcggcaa tgttttgcag
    25921 cggcataccc gccaccgcgc cgccgacaaa cgcgccgacc gtcaggctca ataccacgtg
    25981 cacgcgcgac agcgacagca ccagcataac gattacggca actacgactg cattcattgt
    26041 ttatcgctcc aaacctataa atgtttacat atcgaaacac atcataaccc aataatggaa
    26101 aacccgccaa ttttgcaaac aattatttca aatgcttcat atacttcccc agcgtaaccc
    26161 tgtccaaacc cgccaagtcc ggcagggttt ccactcccga aaaaccattc tccgccaaca
    26221 cgccgcgcac cgccgcgccc tgatcgaaac cgtgttccag caataaaaaa ccgccttccg
    26281 ccaaacggtc gggcgcgcct tgcgccaagg tgcggatgca gcttaggccg tctgaaaagt
    26341 cggtcagcgc gatttgcggc tcaaaccgca aatcgccttg caacaaatgt ttatcgccgt
    26401 tttcgatata gggcgggttg gacacgatga tgtcccattt cccttcagac ggcatatcgg
    26461 tgtcgaacca cgaaccgtgt gcaaattcga cccgcgcgcc caaatctgcc gcatttttcc
    26521 gcgccgtttc aagggcgggc gtgctgatgt cggatgcgcg cacaaacgca tcggggcgtt
    26581 cgagtgcgac ggttacggca accgcgccgc tgcccgtccc caaatcccac acgcgcccgt
    26641 tttcgggcag gcgcgccaat acggcttcga ccaagtgttc ggtttcgggg cgcggaatca
    26701 gcacgctcgg attgactgta aagcgtctgc cataaaattc gcgcacacct aaaatatagg
    26761 caaccggctc gccgttcaga cggcgttgcg ccagcctgtc cgcccgctgt cggacttcgt
    26821 ccggcatttc ttccccgccc cgcgtcaaca actgcacgcg cgtatattcc gaaacatatt
    26881 gcagcagcat tcttgcttca attttaggca gttttgacaa gcccaaccat ttatcaaacg
    26941 tcatttttat cccgtctgcc gctgatgcgg cttttctttc cttattcttt ccggcaaacg
    27001 taccgatggt ggcaaccgca aatgcggcat accacaaata aaatcctgca ccgtagcgca
    27061 caatatccga tgtattccct gcttcatcga cgtatacggc tttcacactg aaagccacca
    27121 acgccaagcc ccaaagtgcc gcatggacag gcacgacctt cttccgcaac gccagcaaaa
    27181 caatggccgc caaccaaaca taattcgcat agaccgcaca atacctgata tccaaagaag
    27241 caaatatcga ccccaaaatc aaaacggtca aaccctccat gcttccatga ttgcccaaat
    27301 aaaatgcaac attggataaa gacgctatcc acagggcaac cgacaccagc aacatcacta
    27361 tgggaaaact tgatttccga ttctgttcct gcatggtttt atcc
//
LOCUS       DAWKOG010000009        26296 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.009, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000009 DAWKOG010000000
VERSION     DAWKOG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26296
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.009"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            814..2262
                     /gene="gnd"
                     /locus_tag="WJK66_000311"
     CDS             814..2262
                     /gene="gnd"
                     /locus_tag="WJK66_000311"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HEZ7578080.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKGTDIVGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFIEQLLPLLDEEDIIIDGGN
                     ANYPDTTRRTHYLAEKGILFVGAGVSGGEEGARHGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGKDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2325..3596
                     /gene="waaA"
                     /locus_tag="WJK66_000312"
     CDS             2325..3596
                     /gene="waaA"
                     /locus_tag="WJK66_000312"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEZ7578081.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMKECRRTGVPLFLANARLSEKSLN
                     GYLKVRSLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFEKRIGDRQVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTVFE
                     TVKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVQVESADAWREAVEKTLSSEGGGMQMQ
                     ARVDGFIAQHRGASARIAEAVREAVCGHRGR"
     gene            3637..4086
                     /locus_tag="WJK66_000313"
     CDS             3637..4086
                     /locus_tag="WJK66_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HEZ7578082.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSCEARRE
                     VEKAMSYRAVRVMPFVVGLLFASGIVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHTVVFTHMLLIVFLAKAMFYISW"
     gene            4119..5093
                     /locus_tag="WJK66_000314"
     CDS             4119..5093
                     /locus_tag="WJK66_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ7578083.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNSSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFKLAGNPGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVQQFEWILYLFGAFLLYTG
                     IHMMKPEGDEEGDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLGFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKQSDK"
     gene            5162..5237
                     /locus_tag="WJK66_000315"
     tRNA            5162..5237
                     /locus_tag="WJK66_000315"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5195..5197,aa:Lys,seq:ttt)
     gene            5457..6710
                     /gene="murA"
                     /locus_tag="WJK66_000316"
     CDS             5457..6710
                     /gene="murA"
                     /locus_tag="WJK66_000316"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEZ7578084.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGV
                     DELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVERLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
     gene            6791..7969
                     /locus_tag="WJK66_000317"
     CDS             6791..7969
                     /locus_tag="WJK66_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEZ7578085.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASI
                     KYCLDNGASVIVMTHLGRPTEGEFHSEDDVAPVAAHLGGLLGKDVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDEMILDIGPKSAAALADLLKAADTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKRGE
                     "
     gene            complement(8037..8285)
                     /locus_tag="WJK66_000318"
     CDS             complement(8037..8285)
                     /locus_tag="WJK66_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ7578086.1"
                     /translation="MLTSEQVKAMIEGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWNAKAQ"
     gene            complement(8376..9293)
                     /gene="ftsX"
                     /locus_tag="WJK66_000319"
     CDS             complement(8376..9293)
                     /gene="ftsX"
                     /locus_tag="WJK66_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HEZ7578087.1"
                     /translation="MSIIHYLSLHVESARTALKQLLRQPFGTLLTLIMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITVYMETAAAQSDSDTVRSLLVRDKRLDNIRFISKEDG
                     LEELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESANMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSAVRPLVDAIFKPYGLNIGWRFFYPGEIA
                     LVFGFVIALGIFGAWLATTQHLLGFKAKK"
     gene            complement(9290..9940)
                     /gene="ftsE"
                     /locus_tag="WJK66_000320"
     CDS             complement(9290..9940)
                     /gene="ftsE"
                     /locus_tag="WJK66_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEZ7578088.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKILFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPSLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(10135..10617)
                     /locus_tag="WJK66_000321"
     CDS             complement(10135..10617)
                     /locus_tag="WJK66_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ7578089.1"
                     /translation="MKYLNLAAITLAATFAAHTASADELAGWKDNTPQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTEANSRNFMKSYGNNVGVLPFTVVEAPKCGYKQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            10741..11094
                     /gene="arsC"
                     /locus_tag="WJK66_000322"
     CDS             10741..11094
                     /gene="arsC"
                     /locus_tag="WJK66_000322"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEZ7578090.1"
                     /translation="MPEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLASARGMMRVKDDLYKELGLDNPDLDNDALLRAIAEHPALLERPIVLANG
                     KAAVGRPLENIEAIL"
     gene            11091..11747
                     /locus_tag="WJK66_000323"
     CDS             11091..11747
                     /locus_tag="WJK66_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEZ7578091.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            11863..13257
                     /locus_tag="WJK66_000324"
     CDS             11863..13257
                     /locus_tag="WJK66_000324"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEZ7578092.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMEWVGLDYDNADNVVYQTRRFDRYKEVIAELLAKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWTDL
                     VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIGATLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPNGKLAELVKPRLA
                     LRDIHETEKPALEDVLALVKDRAQDLNTLADECLYFYVKQTPTEADVQKHWDDEAAAR
                     MLRFAERLEGLEDWNAEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            13278..13679
                     /locus_tag="WJK66_000325"
     CDS             13278..13679
                     /locus_tag="WJK66_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578093.1"
                     /translation="MPSDVGMRLQTAFKRKLNMKKILYFLMFVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQAAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDHAWNAVYGAGERRRRGIDR"
     gene            13676..14167
                     /locus_tag="WJK66_000326"
     CDS             13676..14167
                     /locus_tag="WJK66_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ7578094.1"
                     /translation="MNSLFVDNTVFITRLKAVHIGRLVQALFEEWHGFEPWSSVDKIH
                     AYYGRCLKDGELPLAFAAVDDSGILLGSATVKRHDMESFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWVVVGQHFHNGEWVTVMRLDV
                     DKV"
     gene            complement(14291..16885)
                     /gene="mutS"
                     /locus_tag="WJK66_000327"
     CDS             complement(14291..16885)
                     /gene="mutS"
                     /locus_tag="WJK66_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEZ7578095.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQVDGEPVKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCATHMGSRLLALWLHHPLRNRAHIRARQEAVAALESQYKPL
                     QCRLKNIADIERIAARIAVGNARPRDLASLRDSLFELAQIDLSANGSSLLETLKAVFP
                     ENLSTAEQLRQAILPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTTLPQLQKAAKAAAALDVLSTFSALAKERNFARPEF
                     ADYPVIHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPFDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPEAHAAAVNMHL
                     SALEQGQDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNELENQAAANRP
                     QLDIFSTMPSEKGDEPNVDSFVDKAEEKHFEGILAAALEKLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            17327..18331
                     /gene="gap"
                     /locus_tag="WJK66_000328"
     CDS             17327..18331
                     /gene="gap"
                     /locus_tag="WJK66_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ7578096.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHDIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            complement(18401..18520)
                     /locus_tag="WJK66_000329"
                     /pseudo
     CDS             complement(18401..18520)
                     /locus_tag="WJK66_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(18589..19269)
                     /gene="tsaA"
                     /locus_tag="WJK66_000330"
     CDS             complement(18589..19269)
                     /gene="tsaA"
                     /locus_tag="WJK66_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEZ7578097.1"
                     /translation="MTYTIVPIATARSPYKQKFGIARQPGLVSAAEVCIELNPEFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLNRIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEIRFQIEENRATV
                     IDLSPTPL"
     gene            19384..20019
                     /locus_tag="WJK66_000331"
     CDS             19384..20019
                     /locus_tag="WJK66_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEZ7578098.1"
                     /translation="MIVSIDVDAQKTFTPLCPKELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMVAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPSAKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDMVIVGGLATDYCVKTTVLQL
                     LKGSNWQVIVNEAACRGIAPDTIETAWQEMRSAGAVILKNAEEIKKYINNQ"
     gene            20056..21024
                     /gene="waaC"
                     /locus_tag="WJK66_000332"
     CDS             20056..21024
                     /gene="waaC"
                     /locus_tag="WJK66_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEZ7578099.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEAGFADI
                     ARLHPFVKKIHVMKWRQWRKHLFQAETWREMGHLKQTLRQEAFNFVLDSQGLIKSACF
                     AKMAKSPIYGLDKNSAREGLAALAYAKTYAVPKGKNAVWRNRELFAQVFGYAMPETQV
                     FGLTVPEAGRLKNLAQPYYAALHATSRDSKLWPVENWRSLLQKLNEEQQCNIYLPWGN
                     EDEKTRAKQIADGLPFAIVCDKMNLLQAAYLLKHAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQALMDCVAADKG"
     gene            21251..22000
                     /locus_tag="WJK66_000333"
     CDS             21251..22000
                     /locus_tag="WJK66_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEZ7578100.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDTKLESLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVHIEGGEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLAPADLATDILPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            22011..22946
                     /locus_tag="WJK66_000334"
     CDS             22011..22946
                     /locus_tag="WJK66_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEZ7578101.1"
                     /translation="MSVLIIVEHDNKQLNPTTLHAVTAAAKLGKVDLLVAGSGASAVV
                     ESAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTIFVRPIYAGNAFETVQVDSEKLVLTFRATAFDAAPVQGGH
                     AEVINVEAIPAQNLSRFVNRKLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEVLKN"
     gene            23009..23620
                     /locus_tag="WJK66_000335"
     CDS             23009..23620
                     /locus_tag="WJK66_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEZ7578102.1"
                     /translation="MITTYKTITSSTQAEFKDKGSRFIVFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGVQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVPGLIHAYKEATAQALAVAEVVEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     VLERDLQLDCRLTVSLSLVNYEACVAAWKSTRQIEVNTEKPFE"
     gene            complement(23672..23989)
                     /locus_tag="WJK66_000336"
     CDS             complement(23672..23989)
                     /locus_tag="WJK66_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEZ7578103.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKHAVALIRDMDNFYT
                     HEYQACHRAIEDGAEVDLRTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            24147..25418
                     /gene="purD"
                     /locus_tag="WJK66_000337"
     CDS             24147..25418
                     /gene="purD"
                     /locus_tag="WJK66_000337"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEZ7578104.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVASGNAGTAIEPKLQ
                     NIDLTAHQDLIEFCRKENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARIVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPHTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGKLDSVTAEWSPQTAVGVVL
                     AAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            25452..26054
                     /locus_tag="WJK66_000338"
     CDS             25452..26054
                     /locus_tag="WJK66_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEZ7578105.1"
                     /translation="MTDVLQTVFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
BASE COUNT         6306 a         6045 c         7401 g         6544 t
ORIGIN      
        1 aaagtgggaa tccagaacgc aaaatctaaa gaaaccgctt tcccgataag tttccgtacc
       61 gacgaggctg gattcccgcc tgcgcgggaa tgacggcgga gcggtttctg ttttttccga
      121 taaattccta aaactcaaaa tttcatcatt cccactttca tgggaatgac gggatgtagg
      181 ttcgtgggaa tgacgcggta caggtttccg ttacgtatcc ccatccgaaa gtttatttat
      241 acacaagcaa tccaaagtta tcagactgaa atattgtgag atttacccgc aaatctcatt
      301 ttttccacca cttatacaca aattcggatg cgcactctgc cgatatgcag aaataagaca
      361 ttcgtgccga cacctgacca tttccacaag ttccaaacac gcttccggct taaatctcca
      421 cagtattccc gttcttaaag gcgccagcag ccattcggta aagaaaagta ggatgccatc
      481 tgcacaacaa gcaaactatc cacaaaactt attccccata aaaaacaatc cacaggtatg
      541 cacaaaaggg aatccgcctt acctacaccc taaaaaaagg taatcctgat gatttttaaa
      601 ataaaaaaaa gttacccaca gaaaatttct cctatcaata taatcatcgt ttttttattt
      661 aatttttata ttttatttat aagccttcag ggaaacgcac ggtaaagcgt aaaagcatct
      721 tttcaaaaaa ttaaaaagtt aaggattgtt aaacaagtgt aaatcaggta gagttgcggt
      781 cgataaatta aatctataac ggagcttcct gcaatgaaag gcgatattgg cgtaatcggt
      841 ttggcggtta tggggcaaaa cctgattttg aatatgaacg attgcggatt taaggttgtc
      901 gcctacaacc gaacaatcgg taaggtggac gaatttttaa acggcgcggc taagggaacg
      961 gatattgtcg gcgcgtattc cctgcaagac ttggtcgata agctggcaaa accgagaaaa
     1021 atcatgatga tggttcgcgc aggttcggtc gtcgacgact ttatcgaaca actgctgccc
     1081 ctgttggacg aggaagacat cattatcgac ggcggcaatg ccaattaccc cgacacgaca
     1141 cggcggacac attaccttgc cgaaaaaggg attttgtttg tcggggcggg cgtatccggc
     1201 ggcgaagaag gcgcgcggca cggcccgtcc attatgccgg gcggggataa gcgcgcttgg
     1261 gaagccgtca aaccgatttt tcaggcgatt gccgccaaaa cgccgcaggg cgaaccgtgt
     1321 tgcgactggg tcggcaagga cggcgcgggg cattttgtca aaatggtgca taacggcatc
     1381 gaatacggcg atatgcagtt gatttgcgaa gcgtaccagt ttatgaaaga cggcttgggg
     1441 ctgtcttacg acgaaatgca ccgcgtgttt gccgagtgga acaaaaccga gctggattct
     1501 tatctgattg aaatcacagc ggcaattttg ggctataagg acgaaggcgg tgaaccgctg
     1561 gtcgagaaaa tcctcgatac ggcagggcaa aaaggcacgg gcaaatggac gggcatcaat
     1621 gccttggatt tgggtattcc gttgacgctg atttccgaag cggtattcgc ccgctgcgtt
     1681 tcgtcgttca aagaacagcg cgtgcagacc ggcaagctgt tcgcccgaac cgttacgccc
     1741 gtcgaaggcg gcaaacaaga atgggttgag gcgttgagac aggccctgct tgcatccaaa
     1801 atcatttcct acgcacaagg ctttatgctg atccgcgaag cgggcgaaag ctacggctgg
     1861 gatttggact acggcaacac tgcgctgctg tggcgcgagg ggtgcatcat tcgcagcgca
     1921 tttttaagca atatccgcga tgcctatgaa aacaatcccg atttggtgtt cttgggtgcg
     1981 gatccgtatt tcaaaaatat tttggaaaac tgcctgccgg cgtggcgcaa ggtggttgcc
     2041 aaggcggtcg aatgcggcat ccctatgccc tgcatggctt cggcgattac gttcttggac
     2101 ggctacacga ccgaacgtct gcctgccaac ctcttgcagg cacagcgcga ctacttcggc
     2161 gcgcacactt acgaacgcac cgacaaaccg cgcggcgagt ttttccatac caactggacg
     2221 ggcaagggcg gagataccgc ttcgacaacc tacgatattt gattccgttg ccgaaatgcc
     2281 gtccgaaccg ttcggacggc atttgttttg caggaaatag aaagatgttc caatggcttt
     2341 atgatgtatt gtggctgctt gcgccgatat ggatacggcg ttatttggac aaacgctccg
     2401 gaagtgcccc ggcatatcgg gcgcatcggg acgagcgttt cggcaagccg tatccgaatc
     2461 ccgttaccgg agcggtttgg atacacgccg tttcggtcgg ggaaacgcgt gccgcccaac
     2521 ctttgatacg cgagttgcgg cagcgttttc ccgatgcgcc gctgctgatg acgcagatga
     2581 ccccgacggg acgggaaacc gcgcaagttc tgtttcctga tgcacaatgc cgctatcttc
     2641 cgtatgacaa aaaaacgtgg gtacggcagt ttttgcgcga acaccgcccg atgttcggca
     2701 ttttgatgga aaccgaaatc tggcccaatc tgatgaagga atgccggcgc acgggtgtgc
     2761 cgctgttttt ggcgaatgcg cggctgtcgg aaaaatcgct aaacggttat ctgaaagtcc
     2821 gcagcctgat ccgtcctgcc gccgcttcgc tgacgggctg tctggcacag acggaggcgg
     2881 atgcggcgcg gttggcgaaa ttgggcgcgg catccgtgca ggtgtgcggc aataccaaat
     2941 acgacctgat gccgtcggaa cagatgaaaa cgctggcggg gcagtttgaa aaacgcatcg
     3001 gagaccggca ggttgccgtg tgcggcagca cgcgcgttta tcggggtgaa gacgaggcgg
     3061 aaaaactgct ggcggcgtgg caacaatatc gcggcgatgc cctgctggtc gtcgtgccgc
     3121 gccatcccga gcattttcag acggtatttg aaacggtaaa acgcttcggg tttaaggttc
     3181 agcggcgcag cgacggtttg ccggtcgaac ccgatacgca ggtgtggata ggcgacagta
     3241 tgggcgagct gtatgcgtat tacctgtgcg ccgatgtcgc ttttgtcggc ggcagtctgg
     3301 tcgattcggg ctgtcagaac atcatcgaac cgctttcctg cggcgttccg acgatattcg
     3361 gcttttcgac ctacaatttt tccgaagcct gccggcacgc cttggcatcg ggtgcggcgg
     3421 ttcaagtcga atcggcggat gcgtggcgtg aagccgttga aaaaacctta tcgtccgagg
     3481 ggggcggaat gcagatgcag gcgcgcgtgg acggctttat cgcacaacat cgcggagcga
     3541 gcgcgagaat cgccgaggcg gtgcgggaag cggtatgcgg acatcggggg cgatagtgat
     3601 acaatccgta tccgagtttt ccggttggag catcgtatga gtatttatgc cgtcgcgcac
     3661 atcgtccacc tgtattgcgc catcgccttt gtcggcggcg tgttttttga agtgctggtt
     3721 ttgtccgtcc tgcatacggg acgggtgtcg tgcgaggcgc ggcgcgaagt ggaaaaggca
     3781 atgtcttacc gcgccgtcag ggtgatgccg tttgtggtcg gactgctgtt cgccagcggc
     3841 atcgtgatgg cggcaaaccg ctatctttct atattgggcg aaccgtttgc cacttccttc
     3901 ggtacgatgc tgacgctgaa aatcctgttg gcgttcagcg tgttggcgca cttcgccatc
     3961 gccgtcgtca aaatggcgcg ttccacactg accgtcggct ggtcgaaata catacacacc
     4021 gtcgtcttta cccatatgct gctgattgtc tttttggcaa aagcgatgtt ttatatcagc
     4081 tggtaacaca actacatttc aaatcaggag attgaaccat gacagaatat cccggcatcg
     4141 ggtcgccgtt gttttacggc gttttttttg cggcagtgct ggtcatgatt gccttggata
     4201 tgttttcgct gaagaaaaac agcagccata aagtcggcgt caaagaagcc ttggcatgga
     4261 gcggcttgtg ggttgccgta tcctgcctgt tcgcaggctg gctgtatttc aaactcgccg
     4321 gcaatcccgg ctacggcgcg gcagtcgcaa aagaaaaagt attggaattc tttaccggct
     4381 acattttgga aaaatcgttg gcagtcgaca atattttcgt gttcctgatg atattcggct
     4441 acttcaaagt cgcaccgcag tttcagcacc gcgtgctgct gtacggcgta ttgggcgcat
     4501 tggtattgcg cactgtcatg attttcgtcg gcgcggcact ggttcagcag ttcgagtgga
     4561 ttctgtatct gttcggcgcg ttcctgctct atactggtat acacatgatg aagcccgaag
     4621 gcgatgagga aggggatttg gcaaacagca ggctgctgaa tgccgtcaag aaagtcgttc
     4681 cggtcggcac ggaatttcac ggcgagaaat tttttactgt cgaaaacggt aaaaaaatcg
     4741 ccacgccgct gtttttggtg ctggtcatga ttgaattgag cgatgtcgtg tttgccgtgg
     4801 acagcatccc cgccgtcttt gccgttacca ccgatccgtt tatcgtgctg acttccaata
     4861 ttttcgctat tttgggcttg cgggcgatgt atttcctgct ggcggatgtg gcggaacgct
     4921 ttatcttcct gaaatacggc ttggcattcg tgttgggttt tatcggtgtg aaaatgctgg
     4981 taatgcattg ggtgcatatc ccgatttccg tttcgctgtc ggtcgtgttc ggcgcgttgg
     5041 gtgcatcgat actgacctcg ttaatttata cgaaaaaaca gtccgataaa taaaatgccg
     5101 tatgagcgcc gacaaaatgg ttgacagcat caggcttgat gtatagaatt tcgagcttat
     5161 cgggtcgtta gctcagtcgg tagagcagcg gacttttaat ccgttggtcg agcgttcgaa
     5221 tcgctcacga cccaccagat aaaacaaaaa aagctgagtt ttcaaaactc ggcttttttt
     5281 gtcttccgac aatccttgtt cggaaagcgc ggcatttcgt caaattcttt ggtgcggctt
     5341 ccgtattttt cccgaaaatc ccttataatt ccgcctctgt aaccttcatc gggtgatgct
     5401 ttatgcgttg tcccgagtat tgaaatatta caagtaccca ctaggaaaga acaaacgtgg
     5461 acaaactgaa aatctccgca aacggcccgc tcaacgggga aataacggtc tcgggcgcga
     5521 aaaacgcggc attgccgctg atgtgcgcgg gtttgctgac atcgggtacg ttgcgcctga
     5581 aaaacgtccc tatgctggca gatgtgaaaa ccacacaaaa gctgcttcag gggatgggcg
     5641 cgcgcgtcct gaccgacaat atcagtgaat ttgaaatcaa cggtggtacg gtaaacaaca
     5701 cctgcgcccc ttacgaattg gtccggacga tgcgcgcctc gattttggtg cttggcccga
     5761 cgctggcgcg tttcggcgag gcgcaagtca gcctgccggg cggctgcgcc atcggttcgc
     5821 gccctgtcga ccagcatttg aaaggcttag aagcgatggg tgctgagatt gttatcgaac
     5881 acggttacgt caaagccaag ggtaagctta aaggcacgcg tgtggcaatg gatgtcgtta
     5941 ccgtcggcgg cacggaaaac ctgctgatgg cggcgacgct ggcggaaggt acgacggttt
     6001 tggaaaactg cgccatcgag cctgaagtgg tcgatttggc ggaatgcctg gtcaaaatgg
     6061 gcgcgaaaat cagcggcatc ggtacgtcca caatgattgt ggaaggtgtg gacgagctgc
     6121 acggctgcga acacagcgtt gtccccgacc ggattgaagc ggggacgttc ctgtgtgcgg
     6181 tggcgataac cggcggcagg gtggttttgc ggaatgctgc gccgaaaacg atggaagtgg
     6241 tgttggacaa actggttgag gcaggtgcgg tgattgaggc gggcgacgat tggatcgcca
     6301 tcgatatgcg gcagcgtccg aaggcggtgg acatccgcac ggtcgtccac cccggcttcc
     6361 ccacggatat gcaggcgcag tttatggcat tgaatgccgt ggcggaggga agctgccgcg
     6421 tggtggaaac gatttttgaa aaccgcttta tgcacgtccc cgagttgaac cggatggggg
     6481 cgaacattac aaccgagggc aatacggcat ttgtgcaggg tgtggaacgg ctttccggcg
     6541 cagtcgtcaa ggcgacggat ttgcgtgcgt ccgcaagcct cgttatcgcc ggtttggcgg
     6601 cgcgcggcga aaccgtggtc gaacagattt accacttgga tcgcggttat gaaaatattg
     6661 aaaaaaaact cggcagcgtc ggcgcgaaaa tcgagcgcgt atccggctga tcgggttgcg
     6721 atgccgtctg aagccttcag acggcatcaa ttgtttctgt atcgcaaaca ctgattgaaa
     6781 aggactgatt atggcatttt taaaactgac cgaacaaaac gtgcagggca aaaccgtcct
     6841 cattcgcgcc gatatgaacg tgccgttcaa agacggcaaa atcagcgacg acacccgtat
     6901 ccgcgcctca cttgcgtcca tcaaatactg cttggacaac ggcgcgtccg tcatcgtgat
     6961 gacccacttg ggtcgcccga ccgaaggcga attccattcc gaagacgatg tcgcgcccgt
     7021 tgccgcgcac ttgggcggtc tgttgggcaa agacgtgaaa gtattgaacg actggcgtga
     7081 aaacaaaccg gctttgaacg cgggcgatgt cgtgatgctg caaaacgtgc gtatcaacaa
     7141 aggcgagaag aaaaacgatt tggaactggg caaagcctac gcttctttgt gcgacgtgtt
     7201 cgtcaatgac gcgttcggca ccgcgcaccg cgcccaagcc tcgaccgaag ccgtcgccca
     7261 agccgctccc gttgcctgcg ccggcgtatt gatggcgggc gaactcgacg ctttgggcaa
     7321 agccttgaaa cagcccgcgc gcccgatggt tgccatcgtt gccggcagca aagtgtccac
     7381 caagctgacc atcctcgaat cgctggcgga caaagtcgac caactcatcg tcggcggcgg
     7441 tatcgccaac accttcctgt tggcggaagg taaagccatc ggcaaatctt tggcggaaca
     7501 cgatttggtg gaagaatcca aaaaaattat ggcgaaaatg gcggccaaag gaggttctgt
     7561 gccgctgccg accgatgtcg tcgttgccaa agcctttgcc gccgatgccg aagcggtggt
     7621 aaaagacatt gccgacgttg ccgaagacga gatgattttg gacatcggcc cgaaatctgc
     7681 cgccgcgctt gccgatttgc ttaaagccgc cgacacggtc gtttggaacg gaccggtcgg
     7741 cgtgttcgag tttgaccagt tcgcaggcgg tacgaaagcc cttgccgaag ccattgccca
     7801 aagcaaagcg ttctccattg ctggcggcgg cgacacgctg gcggcgattg ccaaattcgg
     7861 cgttaccgac caaatcggct acatctccac cggaggcggc gcgttccttg agttcttgga
     7921 aggtaaagag ttgcctgccg tagccgcttt ggaaaaacgc ggcgagtaaa cggtttgata
     7981 taaaaaaatg ccgtctgaaa cgttgtgctg tttcagacgg catttttgtg tggcggttat
     8041 tgtgctttgg cattccattc tgccggtgtg gcggctaccg aaatggaaag tgcgtgcagt
     8101 tcgttgcttt ccagttgggc tttaagcccg tctttaatca ggcggtggcg cgcgaggcgt
     8161 gccttgcctt caaattctga tgaaacgatg acggcgaaaa aatgatgtcc gtcgccttct
     8221 acttcgatgt gttcgcattt tgccacgcct tcaatcatgg cttttacttg ttccgaggtc
     8281 agcatggtag gttccttgtt ggaaaaatgc gccattatac tggcaaattg aaatgccgtc
     8341 tgaaaacggg ttcggacggc atttttgacg gtgttttatt ttttggcttt gaagccgagc
     8401 aggtgctggg tggtggcaag ccacgcgccg aatatcccca gtgcgatgac gaagccgaac
     8461 accagtgcga tttctcccgg gtagaagaaa cgccagccga tattaagtcc gtagggtttg
     8521 aagatggcat cgaccaaggg gcgcacggca gagagcagcc aaccgcaaag ccccaagctg
     8581 acggcggcgg aaaggatgct ctgccacatg gcttgataaa ggaatgggcg gcggataaac
     8641 gacgcgggcg cgcccaagag tttggtgatt tcgatttctt cttttcggct gaggatttgc
     8701 agtcggatgg tgttgtgcgc gacaaggacg aacgccatcc ccagcgtcag ggaaagaaac
     8761 cataagattt tacggatgaa ttcgttgatt tggtacagtg tctgtaccca ttccgtatcc
     8821 atgttggcgg attcgaccat aggcagtttg gtaatgtccc ggtagattgc ctgcatttgg
     8881 gcgggcgtgg ttgccgggtc gggggtaacg ataaagacat ccggcagggg gttaccgtca
     8941 agcatggaaa tcagattttg gtcgagattg gactgtaatt cttccaaacc gtcttctttg
     9001 ctgataaagc ggatgttgtc gagccgtttg tcgcgcacca gcaggctgcg gacggtatcg
     9061 ctgtcgcttt gtgcggcggc ggtttccata tagacggtga tttgcggcga ctcattgagt
     9121 ttgcccaaca cgctttgccc gctttggatg cccaagtaca taaacagcgg cagggtcatg
     9181 gcgacggcga gcattatgag ggtaagcagt gtgccgaaag gttggcgcag aagctgtttg
     9241 agcgcggtgc gcgcggattc gacgtgcagc gagaggtagt ggatgatgct catgcgagtc
     9301 gtcctttcga gaggcgcagg atgcggtgtc cgtagtccgc catcagggtt tcgtcatgtg
     9361 cggcgacgat gacggttgtt cccgcttcgt ggaaggtttt gaacaattcc ataatatcga
     9421 gcgcgtaggc gcggtcgagg ttggcggagg gttcgtcggc aatcagcagg ctgggctggt
     9481 gaacgacggc gcgggcgatg cacaggcgtt gttgttcacc gccggagagg gttacgggat
     9541 cgtccaattc tcgtcctttc aggccgactt tttcgatggc gatgcgggca cgctcttcgg
     9601 ctttgcgcgg cggatagccg ataatccgaa gcggcaggat gacgttttgc aggacgttgc
     9661 ggtcgtagag gattttgtgg tcttggaaca cgatgccgat gtgttggcgc ataaagccga
     9721 tttggttgtc ggacaatgtg ccgagatcct gcccgttaaa caggattttg cccctgctcg
     9781 gcttggtgat gcccgaaatc agcttgagga cggtggattt gcccgaaccg gagtgtcccg
     9841 cgataaatat catttcgcct ttgttgattt ggaagctgac gtttttcagg gcttcaaaac
     9901 cgccgggata ggttttggaa acttgttcga aacggatcat aggaggtcct gtaaggaagg
     9961 gcggcatatc gtttcgggcg gttgcgtccg cagggatatg ccgtctgaac aataggtaag
    10021 acagtgccga ttataaagat tccgcctgtt tttagatagt ggataagttt ggcggatacc
    10081 gaaggtcggg cgggaaaaat gccgtctgaa gcggcttcag acggcatccg gcgtttaacg
    10141 gcattttgaa tgggcgagtt tgacggcttc ggtcaggctt ttttcgttca cttccccggt
    10201 aatggtttgt ttgtacccgc atttcggggc ttcgacgacg gtaaagggca gtacgccgac
    10261 attgtttccg taggatttca taaagtttcg gctgttcgcc tcggtgtaac gccaaatcgg
    10321 gtaggaaaca ggagtttgtt tgaggaagtt gccgatattg tcggatgtgt cgagcgcgat
    10381 gccgaccata tcgacgctgc cttttttctg cgctttgtac catttggaca tggcgggcat
    10441 ctcttttcgg cacgggccgc accaggttgc ccaaaggttg acgatgcgta cgggggcttt
    10501 gagtgattgc aggctttgtg gggtgttgtc tttccatccg gccagttcgt ctgccgaggc
    10561 ggtatgtgcg gcaaatgtgg cggcaagggt gattgcggca agattcaagt atttcatcgt
    10621 tttgtccttt gtatgtcgat tcgggtattg taaaccgttt ggtacggttg taggcgggat
    10681 attgagtaaa accctgtgta agccgataat aaggggattg tcgagaagca aggaaatgtc
    10741 atgcctgaaa tcaaaatttt ccacaatccg cgttgcagca aatcgcgtgc cgccttgtcc
    10801 ctattggaag aacgcggcat tgctgccgaa gtggtcaaat atttggatac gccgcccgac
    10861 ttgtccgaat tgaaggatat tttcaacaaa ttgggcttgg catcggcgcg cgggatgatg
    10921 cgcgtgaaag atgatttgta taaggaattg ggtttggaca accccgattt ggacaatgac
    10981 gcgctgctgc gtgccatcgc cgagcatccc gccctgttgg agcgtccgat tgttttggcg
    11041 aacggtaagg cggctgtcgg cagaccgctg gaaaatatcg aagcgatatt gtgaacaaac
    11101 ggctgttttg cagccgcaac ggcttgcgct attacctgtt gggcggtttc tgtttgagtg
    11161 tttttccgct tttgctggtg tttgcctcat ccgtttgggc ggtttatcgg acgggcgggc
    11221 aggttttgcc gccgtatgtc cgggcggatg ccgcgctggt gttgggtgcc gccgcatggg
    11281 acaaacgccc ttccccggtt tttcgggaac gcatcaacca cgccatcgcg ctttaccaaa
    11341 gccgcagggt aggcaaaatc gtgtttacgg gaggccggac caaaaaaggc tatatgaccg
    11401 aagccgaggt cgggcggcgg tacgcgctca aacagggggt tccggcgcgc aacatcctgt
    11461 ttgaaaacac ttcgcgcaac acttatgaaa acctgaacaa catccgtccg gttttgcgtg
    11521 ccaacggtat tgccagtgtg gtgattgtca gcgatcccta ccatttggcg cgggcggcgg
    11581 aaatggcgga ggatttgggt gtccgcgttt atatgtctgc cacgccgacc acgcgctttg
    11641 atgcgggaaa taagaaaaaa atctttatgc tgcaagaggg atatgcgtta tccctctacc
    11701 gtttggaaaa atggggcagc cgctttttgg gatggctgtc tgattgaagc ggtagaaaat
    11761 gccgtctgaa acttatccgc aggcagggtt cggacggcat ttttaatgcc caagcacggt
    11821 aaaaacagta aaataagccg ttttaaagat tctggagcca tcatgaccgt caaaacccgt
    11881 ttcgccccca gccctaccgg ctacctgcac atcggcggcg ttcgcaccgc cttgttttcg
    11941 tgggcgtttg cccgccatca taaaggcgaa tttttattgc gtatcgaaga caccgacttg
    12001 gcgcgttcca ccgccgaatc tgtcaatatc atccttgacg gtatggaatg ggtcggtctc
    12061 gattacgaca acgccgacaa cgtggtgtac caaacccgcc gcttcgaccg ttataaagaa
    12121 gtcatcgccg aactcttggc aaaaggcgat gcctactact gctattgcag caaagaagag
    12181 ctggaagcga tgcgcgagaa agccgaaaaa gaaggcacgg cgacttacga ccgccgctgg
    12241 cggccggaag caggcaaaac cctgcccgaa atccctgccg gcgtgcaacc cgtcgtccgc
    12301 ttcaaaacgc ctttggatgg cgtaaccaag tggacggact tggtcaaagg cgaaatctcc
    12361 atccccaacg aagcactcga cgacctgatt atcgcccgcg ccgacggcac gccgacctac
    12421 aacttctgcg ttgtcgtgga cgactacgac atgggcgtta cccacgttat ccggggcgac
    12481 gaccatgtga acaacacccc gaaacaaatc aacatcttaa aagccatcgg cgctaccctg
    12541 cctgaatacg gccacctgcc gatgattctt aacgaacaag gcaaaaaaat ctccaaacgc
    12601 agcggcgata ccgttgccat taccgatttc ggcgcgatgg gcatcctgcc cgaagccatg
    12661 ctcaactatc tggcacgttt gggctgggcg cacggcgacg atgagttctt cacgatggaa
    12721 caatttatcg aatggtttga tttgaaagac gtttctccat ctccaagccg catggatttg
    12781 aagaaactct actggattaa cggcgaacac atcaaaatca cgcccaacgg caaactcgcc
    12841 gaactcgtca aaccgcgtct cgctctgcgc gatatccatg aaactgaaaa acctgcattg
    12901 gaagacgtgt tggcactggt caaagaccgc gcccaagacc tgaacacact tgccgacgag
    12961 tgcctctact tctacgtcaa acaaacgcct actgaagcgg acgtgcagaa acactgggac
    13021 gatgaagccg ccgcccgtat gctgcgcttt gccgaacgcc tcgaagggct ggaagactgg
    13081 aatgccgaag ccatccacga cctcttcaaa cccttctgcg acgaagaagg cattaaaatg
    13141 ggcaaactcg gcatgcccct gcgccttgcc gtctgcggta cggcgaaaac ccccagcgtc
    13201 gatgctgtgt tggcattaat cggcaaagaa gaagtgttga aacgcatccg cgcttaagtt
    13261 atccacaggt tcgggtaatg ccgtctgatg tcggaatgcg gcttcagacg gcattcaaac
    13321 ggaagttgaa catgaaaaaa atattgtatt ttctgatgtt tgttttttct acaagcgtat
    13381 gggcagggga tgctgaagac aatttgctca gtatccaatc cggttaccgt gccttattgc
    13441 aaaagcaaaa caatctggac ggaaaaatca tcgggatgca gtcggatttg gaagatgcgc
    13501 gccggcgttt gcaggcggct caggcagaca tcgcccgttt ggaagcggaa attcctgcag
    13561 caatggcgca aaaagcccgg caggctgaag agctgaggca aatcggagtg cgtttggacc
    13621 atgcttggaa tgcggtttac ggcgcagggg aacgaaggcg tcggggaatt gaccgatgaa
    13681 ttccctcttt gtagacaata ctgttttcat tacacggctg aaagccgtgc atatcggcag
    13741 gttggttcag gcgttgtttg aggagtggca cggatttgaa ccgtggtctt ctgtggataa
    13801 gattcatgcc tattacggca ggtgtttgaa ggatggcgaa ctgccactgg catttgcggc
    13861 tgtggatgac tccggaatcc tgttgggttc ggctacggtc aagcggcatg atatggaaag
    13921 ttttccacag tatgaatatt ggttgggtga tgtctttgtt ttacctgaat atcgcggaaa
    13981 aggcattggc aggaggctgg tcgcccactg cataggcgca gcgcgttcgc tggggataaa
    14041 gttcttgtat ctttatacgc ctgatgtgca aatattttat gaatcattcg gctgggtggt
    14101 tgtcgggcaa catttccata acggtgaatg ggttacggtt atgcgtttgg atgtggataa
    14161 ggtttaagcc gaatggacag atgccgtctg agctgttcgg acggcatttt ttatatttgg
    14221 aaatttcctg tcagataatg tttatccaca gatttttcca tatggtttga tgctgttccg
    14281 acaacggaaa ttaagatacg gatttgcaca aatctttcag acggtacagt tctgacaatg
    14341 cttcgcgcgg ggtcaggctg tcgggatcga gtttctccag ggctgctgcc aatatacctt
    14401 caaaatgttt ttcctctgct ttgtccacaa agctgtccac attcggttca tctccttttt
    14461 cagacggcat ggtactgaaa atatccagtt ggggacggtt cgcggcggct tggttttcca
    14521 gttcgttcaa atgcttttgg gcggatttca atgcgcgtac aggcaggccg gcgagtttgg
    14581 cgacggcaat gccgtagctt ttaccggcgg gacccggttg gatttggtgc aggaaaacga
    14641 tgtcctgtcc ctgttcgagc gcggaaaggt gcatattgac ggcggcggcg tgggcttcgg
    14701 gcaggtaggt cagctcgaaa tagtgggtgg caaacaggct gaaggatttg tttttttgca
    14761 gcaggtgttc agcaacggcg tgcgcgaggg cgaggccgtc gaaagtggaa gtaccacgtc
    14821 cgacttcgtc cattaaaaca aggctttgtt cggtggcgtg atgcaggatg taggcggttt
    14881 cgctcatttc gaccatgaaa gtggagcggt tggaggcgag gtcgtccgat gcgccgatgc
    14941 gggtgaagat ttgatcgaag ggcccgattg tggcggcatc ggcaggcaca aaacagccgg
    15001 tgtgtgccaa taaaacaatc agcgcgactt ggcgcatgta ggtggatttg ccgcccatat
    15061 tggggccggt gagcagcatg aggcggtgtt tgtggtcaag gtcggtgtgg ttggcggtga
    15121 agtggcgtac ctgctgttcg acaacgggat ggcggccgtt ttcgatgtgg ataaccggat
    15181 agtcggcaaa ctcggggcgg gcgaagttcc gctcttttgc caaggctgaa aatgtggaca
    15241 atacgtccag cgcggcggcg gctttggcgg ctttttgaag ctgcggcaat gtcgtctgaa
    15301 ggtttttcaa tacgccgtca aagagttgtt tttctaaggc gagggcttgc tcttgagcag
    15361 tcagcacttt gtcttcaaag gctttcaact ccggcgtgat gaagcgttcg gcgtttttga
    15421 gggtttgccg gcgttggtag tcggcaggtg cttgttcggc ttgggttttg gacaattcaa
    15481 tgtaaaagcc gtgaacgcgg ttgaactcga ctttgagtgt ggacaaaccg gtacgttcgc
    15541 gttccttggc ttccaaatcc agcaaaaatt cgtcgccatg gttttgaatg cggcgcaatt
    15601 cgtccagttc gggatgaaaa ccgtggttga tgacattgcc gtctttcagc cagacggaag
    15661 gttcgggcaa aatggcttgg cggagctgtt cggctgtgga taggttttcc gggaaaacgg
    15721 ctttgagggt ttccaacagg ctgctgccgt ttgcggacaa gtcgatttgt gccaactcga
    15781 acagactgtc gcgtaaagat gccaaatcgc gcgggcgggc gttgccgacg gcgatgcggg
    15841 cagcaatgcg ttcaatgtcc gcaatgtttt tcagacggca ttggaggggt ttgtattggc
    15901 tttccagcgc ggcaacggct tcttggcgcg ctcggatgtg ggcgcggttg cgtaaagggt
    15961 ggtgcagcca gagagccaag aggcggctgc ccatatgggt agcgcaaagg tcgagcgtgg
    16021 acatcagggt cggcgatttt ttgccggaga gggtttgcgt gatttcgaga ttgcggcgcg
    16081 tggcggcgtc catgccgata tattggctgt cggtttcgag cgacaggccg tccaaatgtt
    16141 gcggcatcag gttttgcgtc aggcggatat agttcaacag cgcacctgcc gcgccaatcg
    16201 caacggcgtg ttctttgccg tccaaaccga agccgcgcaa atcctggcag ccgaaatatt
    16261 ccgtcagcag tttttccccc gcgtcggcgg caaactgcca cgcgttcagg cgcgtaacac
    16321 ccgatgccgt ctgaagttgc ggtgcgtttt tactgtccgg caacagaatt tccgccgcct
    16381 gcaggcgcgc cagttcgtcg tccaatttat ccacagttgt cagcttggtt ttgaattcgc
    16441 cgctttgcag cgatgcccac gccaaaccga tgtatttttt gtcgggggac acggcaacga
    16501 tacggttggt ttccttgtct tccagcaatg cggaatcggt cagcgtgccg ggcgttacga
    16561 tgcgcacgac tttgcgctcc acaggccctt tgcccgcgcc gacttcgccg acctgttcgc
    16621 aaatcgccac gcttttgccc aacttgacca ggcgcgccag atattgttcg gcggcgtgaa
    16681 acggcacgcc tgccattttg accggctcgc catccacctg tccgcgcgtg gtcagggtaa
    16741 tatccaaaag ttttgccgct tctaccgcat cgtcgaaaaa catctcgtaa aaatcgccca
    16801 tacggtaaaa caccagtttg tcggtatgtt gcgctttgat gccgaggtat tgctgcatca
    16861 ttggggaaac ggcggatttg gacatggggc tgccttatca ttaggaaaac ggtattgtag
    16921 caaaaggttt tgtcttcgac ggaatgagac tgaccgtaag cctgtcaata aggttgtgga
    16981 gacaactagg aaaacagcaa agttatccac agaaatatta ttaagtcaaa aaacatccac
    17041 gcttatctgt cttatacagc aagtcttaaa ctcaaactta tttgacaaaa agataatgaa
    17101 aatattggat atttgctact tacgcacaag caggttggca atcatcaaaa tcatcattaa
    17161 aatttaaatt caaatattga tggtaaaggt tttggaaatc tgtgcaaaca gcaaaattca
    17221 tctatatcaa ataagcgtca agtagttaga tgtaatagtg gaatgtaact ttatttcaga
    17281 atacaatgca ggcatcggac tttatttcac aacctggaga caaaatatga gcattaaagt
    17341 agcgattaac ggtttcggcc gcatcggccg cctcgcattg cgtcaaattg aaaaagccca
    17401 cgacatcgaa gtcgtcgccg tcaacgacct gacccctgcc gaaatgctgc tgcacctttt
    17461 caaatacgac agcacgcaag gccgtttcca aggtactgcc gaattaaaag acgatgccat
    17521 cgtggtaaac ggcaaagaaa tcaaagtttt tgccaatccg aatcccgaag aattgccttg
    17581 gggcgagttg ggtgtggatg tcgtcctcga atgtaccggc ttctttacca acaaaaccaa
    17641 agccgaagcc cacatccgtg ccggcgcgcg caaagttgtg atttccgcac ctggcggcaa
    17701 tgatgtgaaa accgtcgtat atggcgtaaa ccaagacatt ttggacggca gcgaaaccgt
    17761 catttccgcc gcttcctgca ccaccaactg ccttgccccg atggcggcag tcctgcaaaa
    17821 agagttcggc gtagtcgaag gtctgatgac caccatccac gcctacaccg gcgaccaaaa
    17881 cacccttgac gcgccgcacc gcaaaggcga tttgcgccgc gcccgagccg ccgcgctcaa
    17941 catcgtgccc aacagcaccg gtgccgccaa ggccatcggt ctggttatcc ccgaattgaa
    18001 cggcaaactc gacggctccg cccaacgcgt ccctgttgcc accggctcgc tgaccgaatt
    18061 ggtttccgtc ctcgaacgcc ctgtaaccaa agaagaaatc aacgccgcga tgaaagccgc
    18121 cgccagcgaa tcttacggct acaacgaaga tcaaatcgtt tcttccgacg ttgtcggcat
    18181 cgaatacggc tcgctctttg acgcaaccca aacccgcgtg atgaccgtgg gcggcaaaca
    18241 actggtgaaa accgttgcct ggtacgacaa cgaaatgtcc tacacctgcc aactcgtccg
    18301 cactttggaa tacttcgcag gcaaaatcta aggcttgaac aaacctgtgg ataagtaaat
    18361 gccgtctgaa cacattgcgt tcagacggca ttgtttatct gtatagtgga ttaaatttaa
    18421 accagtacgg cgttacctcg ccttgccgta ctatttgtac tgtctgcggc ttcgttgcct
    18481 tgtcctgatt taaatttaat ccgctataac ttgatattgc ggcttatggc gaacaaacaa
    18541 cagccgtttg ttcaactgct atgcggcaaa accggatttt tgcccgattt aaagcggggt
    18601 tggggaaaga tcaataacgg ttgcacggtt ttcctcgatt tgaaatctga tttcgtaatc
    18661 cgcaatattc atcacataaa tccgttcggg aatattctga taggcggggc gcggatcttg
    18721 ggcaatgctt tggctgataa ggtttttggt gtttgcagat aaattttccg cgccgatgtt
    18781 ttcctgccaa acgacttcca actctacggg tttgccgctg acgaaaccag atgcggcatc
    18841 gggtttggat tcgacaaagg ggatataagg cttgatgtcc acaatcggcg tgccgtcgag
    18901 caggtctgcg ccgctgcaat aaaggcggac gggtttgccg gtttcgatgc ggttgagttt
    18961 taagagtgat agtccgagat ggttggggcg gtgggggctg cgcgtggcga atacgcccat
    19021 tttttgtttg ccgccgagcc gtggcgggcg caccatttgt gcccagcctt catccaatac
    19081 gccgtgaaaa ataaaactta tccacacata atcgaaatct tccagcccgc gcacgctgtc
    19141 tgcggtaaat tcgggattca gctcgatgca tacttctgcg gcggagacta aaccgggctg
    19201 gcgggcgatg ccgaatttct gtttgtaggg cgatcgggcg gtggcgatag ggacgatggt
    19261 gtaagtcatg aggattgtgg ataagttttt cggaaaaacc ttatcttatc acagcctgtt
    19321 ttaccgcttt tgggcagtaa tttgttataa tacagggcat tttaattgcg ttgaaatgat
    19381 gagatgattg tttccattga tgttgatgca caaaaaactt ttacgccttt gtgtcctaaa
    19441 gagctgcctg taaacgaggg gcatttgatt gtcgaagaat tgaatgccca agccgctttg
    19501 gcggatttgc gcgtgatgac gaaagatgcg catcatatgg tagcgaaatg gcttgtggat
    19561 aaccctgttg atatgttgaa gccgacaggt ttgcctgatg cggatttgac ttgggtggct
    19621 catgcgatgg tcggtacgcg cggctatgaa ttattggacg gactgccttc tgccaaagaa
    19681 tacgattatt gcgtttggaa aggtgttgat cctgaattgc atccttacgg cgcgtgtttt
    19741 cacgatattg aggaaaaact gagcactgga ttgattgagt ggctgcgttg tcaaaatacg
    19801 gatatggtca ttgtcggcgg tttggctacg gattattgtg ttaaaacaac ggttttgcag
    19861 ttgctcaaag gtagcaattg gcaggttatc gtcaatgaag cggcttgtcg gggtattgcg
    19921 ccggatacca ttgaaacggc atggcaggaa atgcgttctg ccggtgctgt catcttaaaa
    19981 aacgccgaag aaattaaaaa atatattaat aatcaataag ttaaaatcgt ttcacgtgaa
    20041 acatacttct taaatatgaa aattttgctt gtccgcttgt ctagtatggg cgatttaatt
    20101 cacactttgc ccgcaatcga agatttggcg cgacaatgtc ctgatgtaga actgcattgg
    20161 ctatgtgagg ctggatttgc agatattgcg cgcctgcatc cgtttgtaaa aaaaatccat
    20221 gtgatgaaat ggcggcaatg gcgcaaacat ctctttcagg ctgaaacttg gcgggaaatg
    20281 ggtcatctga aacagacttt gcggcaggaa gcatttaatt ttgtattgga cagtcaaggt
    20341 ctgattaaaa gcgcgtgttt cgctaaaatg gcgaaatccc ctatttatgg tttggataaa
    20401 aatagcgcgc gcgagggact ggctgcgctg gcgtatgcga aaacatacgc tgtaccgaaa
    20461 gggaaaaatg ctgtttggcg caatcgtgag ctgttcgccc aagtatttgg gtatgcgatg
    20521 cctgaaacgc aggtatttgg cttgactgtt cctgaggcag gccgtctgaa aaatttagcg
    20581 cagccgtatt atgcggcttt gcatgcgacc agtcgggaca gtaagttatg gcctgtggaa
    20641 aactggcggt cgttgctgca aaagttgaat gaagaacagc aatgcaatat ttacctgcct
    20701 tggggaaatg aagatgaaaa aacgcgtgcc aaacaaattg cagacggact gccgtttgct
    20761 attgtgtgcg ataaaatgaa tttattgcag gcagcgtatc tgctgaaaca cgcggtcgga
    20821 attgttggcg tggataccgg tttgctgcat ttggcaaacg ccttggaaaa acctgtggtc
    20881 ggcatttata ccgataccga tccgattaaa acaggcgttc aggtttcgcc aattgcaaaa
    20941 aatttgggca atatcgggca gattccgacc gcagatttgg tttatcaagc gctgatggat
    21001 tgtgttgcag cagataaagg ctaaagggtc gtctaaaact gatatggcgg catgatattt
    21061 tccaatttat tagatacagg aatgtatatc cgctagtctg agtaaatgct ttaaaaatgg
    21121 tataatcggc aagcattatc cgaaagattt ttgaatgaat tcgtttttaa ggaaaacgga
    21181 tttggtttta tagggaagaa ggtttggttt cgctggatgg tgaaagcagc gttcttttta
    21241 ggagggttca atgaaagcac tggtcgcagt aaagcgcgta gtggactaca acgtcaaagt
    21301 tcgtgtaaaa gccgatggtt cggatgtgga tatcggcaat gtcaaaatgt cgatgaatcc
    21361 gtttgacgaa atcgctgtgg aagaagctgt ccgtttgaaa gaagccggaa aagtaagcga
    21421 aatcgtagcg gtttctttgg gtgagaaaaa atgcgaagaa accttgcgta cagctttggc
    21481 gatgggtgcc gaccgtgcca ttcatgttga aaccgatact aaactggagt ctctggccgt
    21541 tgctaagttg ctgaaagccg ttgcggacaa agaaaatccg caaattttct ttttgggcaa
    21601 acaagcgatt gatgatgatg ccaatcaagt ggcgcaaatg ctggcagctt tgctgaatgc
    21661 ggcgcaaggt acgtttgctt caaaagtaca cattgaaggt ggtgaagtac agattgtgcg
    21721 cgaaattgat ggcggcgaag aaaccatagc attaaaactg cctgctgtta tcagtgctga
    21781 tttgcgtttg aacgagccgc gctttgtcaa actccccaat attatggcgg caaagaaaaa
    21841 acctctggaa aaactggcgc ctgccgattt ggctaccgat attttacctc gtctgaaaac
    21901 ggtgaaattt gccgaaccta aggcgcgtca ggcaggcgta aaagtagcaa gcgttgccga
    21961 attggttgaa aaattgaaaa acgaagccaa agtgatttga ggaggctgaa atgagcgtat
    22021 tgattattgt cgaacacgac aacaaacagt tgaatcctac cactttgcat gctgttaccg
    22081 ctgccgccaa gctgggtaaa gtcgatttat tggttgccgg aagcggtgca tctgccgtag
    22141 tagagtccgc aaaacaagtg gcgggtgtgg aaaaagtttt ggttgcagat gctgcccatt
    22201 atgccgaagg tttggctgaa gagttggctc cgctggttgt taaattggca gcggattacc
    22261 gctatgttgc agcaacggca actacattcg gtaaaaacct tttgcctcgc gtagcagcct
    22321 tattagatgt accgcaaatt tctgatttga ccgagattgt ggataacacg atttttgtgc
    22381 gccccattta tgcagggaac gcatttgaaa ccgtgcaagt tgattcggaa aaattggtgc
    22441 tgaccttccg tgcgacggct tttgatgcag ctcctgtaca aggtggtcat gccgaggtaa
    22501 ttaatgttga agcaatccct gctcaaaacc tgagtcgttt tgtgaaccgc aagctttctc
    22561 attccgatcg tcctgaactg actcaggcaa aagtgattgt ttccggtggt cgtgcgttgg
    22621 gtagtgcgga aaaattcaat gaagtgctga caccgttggc ggatgtttta ggtgcggcaa
    22681 tcggtgcatc ccgtgcggcg gttgatgccg aatatgcgcc aaacgatgct caagtcggac
    22741 aaaccggtaa agtagttgcg ccgcaactct attttgcaat cggtatttca ggtgcgattc
    22801 aacacgttgc cggtatgcaa gacagtaagg tgattgttgc aatcaataaa gatgctgatg
    22861 cgccgatttt caatgtagcc gattacggat tggttggcga tttatttgaa atcgttccac
    22921 aattaacaga agtattgaaa aattaatgtt tcacgtgaaa cgttcagacg accttgttat
    22981 taatgaggtc gtctgaaaac atttcagcat gattacgact tataaaacca ttacctcctc
    23041 gacgcaggct gagtttaaag ataaaggcag ccgttttatt gtatttgcct atccgattcg
    23101 gacattggct gatgtgaaaa aatacctcga tccgttaaag gaagagcatc ataaagcacg
    23161 acactggtgc tatgcctatc gtttgggtgt ggatggtgtg caatttcgtg ccaacgatga
    23221 tggagagcca tcaggaagtg ctggacgacc aattttggga cagattgatt cggtgggtat
    23281 taccgacgtt ttggtcgtgg tcgtccgcta tttcggtggt actttgttag gtgttccagg
    23341 tctgatacat gcgtacaaag aggcgacggc tcaagcgttg gcagttgcag aagtagttga
    23401 aaagaatatt gaaaaaactg tttggctgaa atgtgaatat ccgtttttga atgaagcgat
    23461 acgcattgct aaacaatatc aggcggatgt attggagcgg gatttgcagt tggattgccg
    23521 attgacggtc agtttgtcgt tggtaaatta tgaggcctgt gttgcggctt ggaagagtac
    23581 acggcagatt gaagtaaata cggaaaagcc ttttgaatga aaggtcgtct gaaatgcttt
    23641 gtgatggtat ttcagacgac ctcttcgctg actaatcttc ttgtgtatat ttgtctaagg
    23701 catcgacgct ggagcgcaaa tataaccaag ccagttgatg gaattcgccc agcgcgttcc
    23761 atagtgcatc ttcactcata atgctgtatt cgccttctgt gcgcaaatcc acctccgctc
    23821 catcttcaat agcccgatga caagcctgat attcgtgggt ataaaaattg tccatatcgc
    23881 gaatcaaagc gacggcatgt ttccaacggc ggatgtcttc ttccaattgc ggtagtaaat
    23941 gacaccattc gcggtatttg ctttggattt catcaatacg tttttgcatg gttgcttgtc
    24001 cttttttaat ttatgaataa gttgaatagg ttgcagagtg gaatgaagcg gtcttttgtt
    24061 tgattctact aaaatttctt cagatgggat aaaagtccct ttgtatcgag ttttcgttaa
    24121 aagtacatat ttgtcaagga gatggcatga aactgctggt tatcggtaat ggcggtcgcg
    24181 aacacgcgct ggcttggaaa ttggcgcagt cgcctaaagt ggaaacagta tttgttgcgt
    24241 ctggtaatgc cggtacagcg attgaaccca agctgcaaaa catcgacttg actgcgcatc
    24301 aggatttgat tgaattctgt cgtaaagaaa atattgtttt taccgtcgtc ggccctgaag
    24361 cgcctttggc ggcaggtgtt gtggatgatt tccgtgctgc aggactaaaa atattcggtc
    24421 cgacacaata tgcggcgcag ttggaaagtt ccaaagattt cgccaaagca tttatggcga
    24481 aatacaatat tccgaccgcg caatatcaaa cctttgaaaa cgccgatgcc gcacatgatt
    24541 acgtcaatca gaaaggtgcg cctatcgtta tcaaagccga tggtttggcg gcaggtaaag
    24601 gcgtgattgt ggcgatgact ttggatgaag cgcatgctgc gattgatgat atgctgctgg
    24661 acaacaaaat gggcaatgcc ggcgcgcgta ttgtgattga agatttcctg caaggcgaag
    24721 aagcgagttt tatcgtcatg gttgatggca ataatgtact gcctatggca accagccaag
    24781 accacaagcg tcttttagac ggcgacaaag gtctgaatac gggcggtatg ggtgcgtaca
    24841 gccctgcgcc tgtggtaacg cctgttgtgt acgaacgcgc gatgaatgaa attattttgc
    24901 cgaccgtagc agggatgaaa gcggaggggc atgagttcac cggtttcctg tacgcaggtt
    24961 tgatgattga tcaaagcggt gcaccccata cgattgagtt taactgccgt ttcggcgatc
    25021 ctgaaaccca gccgattatg agccgactga acagcgactt atcggatttg gttgaagcgg
    25081 caatagacgg caaactcgat agcgtaaccg cggaatggag cccgcaaact gccgtgggcg
    25141 tggtactggc ggcgcaaaat taccccgaaa cgcccaaaaa aggcgatgtt atttccggtc
    25201 tggatgctgc caaccaagta ggcaaagttt tccatgcagg tacaacggca aacgagaaag
    25261 gtgacgtatt gaccaacggc ggacgcgtat tgtgtgttgt gggattgggc gataatgtgg
    25321 cgcaagcgaa agctaaggca tatggcgcgt tggaaaaaat cagtttcgac gggatgcaat
    25381 accgtaaaga cattgccgat aaagcgatta accgttaatt agaaagagga tgatgccgtc
    25441 tgaagccaag tatgactgat gtgcttcaga cggtatttta tattatgtta tttccgagaa
    25501 ttatcgcagc atcagcgcaa cgtaagttaa gcgtttattt gaaaaaaggc ggtttggtcg
    25561 cttatccgac cgaatcttgt tatggcttgg ggtgtcttcc gacattagcc aaagcactcg
    25621 gtaagttggc gcatttgaaa aaaagaccgc aacacaaggg tatgattgtc atcgggaatc
    25681 aatttgagca attgcagcct ttacttcaaa tgccgtctga aaatctgcag gatatgctta
    25741 gaaaagaatg gcccgcccct aaaacgttcc tattatccgc caaatcctgt gtcttacctg
    25801 agttgcgcgg gaaacagcgc agcaagttgg cggtcagggt tcccgcccat gtaggtgcgc
    25861 gccgcttgtg tcaggctttg caaacgcctt tggtctcgac ttcgtgcaac cgcgcaggca
    25921 aacgcgcgtg tcggacggag agggaagtca gaaggcaatt cggacgcgat gtgtggattg
    25981 ttggtgggag aatcggcagg cagaaatcgc cgagtcagat tattgacggg gaaacaggta
    26041 aacgtttgcg ttagatatgg gtacttgaat gaaaactttt tttgcataca caaagaaaaa
    26101 atttttcgga tagcatttct ctacatgatt gttattgcac agagataaag gtagaaggac
    26161 gcaacgtctg ctttaatttt gaggacggtt ttgtcgttcc ggacaataat ccgaacaatc
    26221 aagcgggcaa gcatctgaaa acagattttt cccgtgttgt tttaatccat aaaaatgaaa
    26281 acgccgaaga tgatta
//
LOCUS       DAWKOG010000010        23663 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.010, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000010 DAWKOG010000000
VERSION     DAWKOG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23663
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.010"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(66..905)
                     /locus_tag="WJK66_000339"
     CDS             complement(66..905)
                     /locus_tag="WJK66_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ7578106.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADGAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1190..1265
                     /locus_tag="WJK66_000340"
     tRNA            1190..1265
                     /locus_tag="WJK66_000340"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1223..1225,aa:Arg,seq:ccg)
     gene            complement(1330..2523)
                     /locus_tag="WJK66_000341"
     CDS             complement(1330..2523)
                     /locus_tag="WJK66_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ7578107.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVSRAETARAAAPAPSPYLPMEGLDTYRSAVQHLLFGKDNRALVQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFTYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(2544..3632)
                     /gene="trmA"
                     /locus_tag="WJK66_000342"
     CDS             complement(2544..3632)
                     /gene="trmA"
                     /locus_tag="WJK66_000342"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HEZ7578108.1"
                     /translation="MSDYTQQLQDKKDHLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKAGGASLIRCDRFEAASEAVNRLMPELIAVAAQSAELRNRW
                     YAVEFLSTLSGEMLVTMIYHKRLDNEWMQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSVSAAQWNIEANRIGNIKIARLSAEEFTEAYTGKREFKRLK
                     DGGIALTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRANLDTLAETH
                     AVERAALFDQFPFTHHIESGVLLKKKIL"
     gene            3775..4875
                     /gene="aroC"
                     /locus_tag="WJK66_000343"
     CDS             3775..4875
                     /gene="aroC"
                     /locus_tag="WJK66_000343"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEZ7578109.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLALTESDIQAD
                     LDRRKPGTSRHVTQRREADQIEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIAQSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFEGCEHISQNPFFAANHSQIAELENYMDSVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDICVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVAVDTPDIARSDK"
     gene            4966..5391
                     /locus_tag="WJK66_000344"
     CDS             4966..5391
                     /locus_tag="WJK66_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HEZ7578110.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDSALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAVAETPSVEAEAAESSAAE"
     gene            complement(5465..7450)
                     /gene="parE"
                     /locus_tag="WJK66_000345"
     CDS             complement(5465..7450)
                     /gene="parE"
                     /locus_tag="WJK66_000345"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEZ7578111.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPLFSCENYISDEHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVNNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVATVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDSILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(7517..8041)
                     /locus_tag="WJK66_000346"
     CDS             complement(7517..8041)
                     /locus_tag="WJK66_000346"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEZ7578112.1"
                     /translation="MLDREGYRPNVGIILTNNRDEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLPQHVKIVGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIEFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQPSGNR"
     gene            8206..9501
                     /gene="serS"
                     /locus_tag="WJK66_000347"
     CDS             8206..9501
                     /gene="serS"
                     /locus_tag="WJK66_000347"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEZ7578113.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYDFDTARFDALEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPAGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLKHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDESKTTQYLIPTAEVTLTNTVAGSILDEGGLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMG
                     FGATKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(9579..10577)
                     /locus_tag="WJK66_000348"
     CDS             complement(9579..10577)
                     /locus_tag="WJK66_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ7578114.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEAEKIGGRYVELNELYARSDIITLHCPSTPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSSAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10702..11778)
                     /gene="prfA"
                     /locus_tag="WJK66_000349"
     CDS             complement(10702..11778)
                     /gene="prfA"
                     /locus_tag="WJK66_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEZ7578115.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIGALDTELQKLLLP
                     KDADDDKNIFIEVRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIIGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PVDLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(11882..12661)
                     /locus_tag="WJK66_000350"
     CDS             complement(11882..12661)
                     /locus_tag="WJK66_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ7578116.1"
                     /translation="MNFTDTHCHLADAALRENLPHILTAAREAGVGRFIVPATRPQDW
                     QDVADLAQRLSENPLWGNIHIALGIHPWFSDDVSEPDFQLLEQTLKAHPTAWVGEIGL
                     DFYDKTQTPPQRERQIQVFVRQLEIAQTLRRRVIIHNLKATADIAAAVKQTGFAQGGI
                     VHAFSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLK
                     KEINTPANIPGIAKIAAEIRGTCVEEIAKVTERNADALLGR"
     gene            complement(12776..13765)
                     /locus_tag="WJK66_000351"
     CDS             complement(12776..13765)
                     /locus_tag="WJK66_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEZ7578117.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVGQALAPFSNGMDFD
                     WHVCGTLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANLFALALQGL
                     DKPIVLTGSQWPYNAVGSDAPRNLATAVAAFPLGLKQTLIAFDGKLYPAVGSSKVSTE
                     TAAGFDNPHFGALAEWDETQGWHNVRIPNQDAADVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAHALILQSYGHGNTPADEGFIRAVQDFTQQGKLLLNISQVRQGR
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSQGWGKEEVEKELARLKL"
     gene            complement(13832..14515)
                     /locus_tag="WJK66_000352"
     CDS             complement(13832..14515)
                     /locus_tag="WJK66_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ7578118.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLVGDGIMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(14704..16038)
                     /gene="glmM"
                     /locus_tag="WJK66_000353"
     CDS             complement(14704..16038)
                     /gene="glmM"
                     /locus_tag="WJK66_000353"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEZ7578119.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVIQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEIELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPSHSDLRGLKLVVDTANGAGYGVAPKVFHELGAQVVSIGDEPNGYNINEK
                     CGATYTKTLQAAVLQHEADYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQILNQDLATVCADWQPYPQTMINVRIQKGQKWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWAREGAERIASAIGNI"
     gene            complement(16168..17025)
                     /gene="folP"
                     /locus_tag="WJK66_000354"
     CDS             complement(16168..17025)
                     /gene="folP"
                     /locus_tag="WJK66_000354"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEZ7578120.1"
                     /translation="MARHVWQAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRAGADYVSPEEEWARVEPVLAEVAGWGVPVSLDTRR
                     TVVMEKALALGGIDIINDVAALTDEGAVELLARQTDTGICLMHMQGLPENMQINPKYQ
                     DVVGEVARYLKARAAECVAAGIAPQRITLDPGFGSGFGKTLQHNIALMRHLPELMAET
                     GLPLLVGVSRKSTIGELTGEADAAARVHGSVAAALASVARGAQIVRVHDVKATADALK
                     VWEALGINL"
     gene            complement(17165..18058)
                     /locus_tag="WJK66_000355"
     CDS             complement(17165..18058)
                     /locus_tag="WJK66_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEZ7578121.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVRIAAEP
                     SVWRNADAVFLDPFFTSANLYDTSSADEGRGRCRRLVEQTASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLSHTKDHPYRLDVESIVKVRQISHLPVIANITGLWNRDMPQEILY
                     GLAKAAGACGIVGTCFEKAGG"
     gene            18149..20263
                     /locus_tag="WJK66_000356"
     CDS             18149..20263
                     /locus_tag="WJK66_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEZ7578122.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAVGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDQTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDSQKRQASVNRIIVENSTVRLNF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSDGIGTPKISPF
                     HFEASTSLDGHGITISTTGTPAIRFNAGGADAAGLGLRADTSFRNLHLTAQIPALALR
                     NNSIKIETVNGAFTAGGEYAQWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLDAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKDHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            complement(20327..21805)
                     /gene="ubiD"
                     /locus_tag="WJK66_000357"
     CDS             complement(20327..21805)
                     /gene="ubiD"
                     /locus_tag="WJK66_000357"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEZ7578123.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRIAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPVKPDGTRYGYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGENVDLYKLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDHFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVIGMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETEREWGRVIKKDPAVTAKIDGIWGELGL"
     gene            complement(21825..22340)
                     /locus_tag="WJK66_000358"
     CDS             complement(21825..22340)
                     /locus_tag="WJK66_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HEZ7578124.1"
                     /translation="MYTCTSCGEKHEDMPAIGFTAPYPYNQLSEEERIAYQAECGGDF
                     CIIRYPDQTDRFIRAVLLIPIIGHEETLEYGVWVSVSEKSFNDYQSHFHDNPENVVYF
                     GMICNRLPPYETDTFGLHCNVVTQPDGQRPLLQLHQGSEHPLVRDFYHGIEYAEAQAR
                     IGAVSESDCSG"
     gene            complement(22347..22847)
                     /locus_tag="WJK66_000359"
     CDS             complement(22347..22847)
                     /locus_tag="WJK66_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578125.1"
                     /translation="MVFVNPLYYGREAGWFVWLAAAMCGLWLLRALTAKLPQQRYAAL
                     FITLFFAAVLVFGRHDSMYWYPVLVNLMMLAVFGGSLFAGQTVIEKLARLQQPDLPEK
                     AVRYTRRVTQVWCVFFIANGTLAALLARLGRYDWWAVYTGVIAYVLMGMPFAGEWLYR
                     KLVLKV"
     gene            complement(23079..23648)
                     /locus_tag="WJK66_000360"
                     /pseudo
     CDS             complement(23079..23648)
                     /locus_tag="WJK66_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691695.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
BASE COUNT         5367 a         6783 c         6179 g         5334 t
ORIGIN      
        1 tcccataaaa aaaacggagc agatacctgc cccgttttta tttaatccga aattttaatc
       61 taaatttaga attttgcacc ggattggttt gccatatagt caacagccgc tttgacttcg
      121 tcatcgctca aacctgcatt gccgcctttg gcaggcatcg cgttaaagcc ttcaagggcg
      181 tgtttgtgca aggtttcttt gccttttttg atacgcggtg cccaatcgtc ttttttgcct
      241 atgccgggaa taccgggaat cgaaccgccg tggcacacct gacaggttgc ttcgaagact
      301 tttttaccgt caacgccgac cgcaggggct gccgcaccct tgtcttctgc cttcgcttct
      361 gccggagctg caccatcggc aggagcagaa gctgttcctg aagcggcatt gtcggcaggc
      421 gcagcctcat caggattcgg gaaagaaccg ccgcttttgt tcgccatgta agtaatcgcc
      481 cgtttgagtt cctgatcggt caggtctgcc gcaccgcctt tggcaggcat ggcgttaaag
      541 ccgttcagcg cgtgttggaa caaggtatcg aagccttgcg cgatacgcgg tgcccaatcg
      601 ccgttgtgtt ccagtttcgg agcgttcggc acattgctgt ccgccgcgtg gcattggata
      661 cagattttgc cgaaaatctg ttcgccttgg cgttcgccga cggggatgcc gtcgcccatc
      721 gtcaattgtc cgacgggctg gatacgggtc tgcgttgctg cttccgtagt ggcatcgaca
      781 tcgccgaacg agccgctgcc cgccagctta atcaggaaat aaaggactgc aataacaata
      841 acgataccgc tcacaagggt aaacagtgca gagccttggg ctttgttgtc gcggagttgt
      901 ttcatttggt aggcctcgcc gtcaggttag gttgtgctgt aaattatagt ttggtgtgtt
      961 aaacgcagtt aacaatattt tgctggatta tactgaattc acagggtctt tccaatcgct
     1021 atcattgaaa atatgaaaaa atttgccaac ggtatctgta taaaacaaac aatcctttga
     1081 aaataatggt ttatcctcaa gaaaactctc cttatgccgc catacgccgc ctgccggcgc
     1141 aagataacct ttgccaattt gcagaattta cgttaacctt acgttttacg cacccatagc
     1201 tcagttggaa gagtgtcagt ttccgaagct ggaggtcaca ggttcgatcc ctgttgggtg
     1261 cgccaattat aaagaggccg tctgaaagat aaatattttt cagacggtct tttgacttac
     1321 ttcaaactct tatttcaaga cttccgcaaa tgcgcgggca acatagtcgg tattcgacgt
     1381 attcagtccg gcgacgcaca tcctgccgga atccagcagg taaacggcaa attcgtcgcg
     1441 cagcctgcgg acttgttcca cgctcaatcc tgtgtagccg aacatgccgc gctgtttgat
     1501 gaaataagtg aaatcgcgat cggggatttg cgcagttaag acatcataaa gtttctgccg
     1561 catcgcacgg atgcggtcgc gcatcatata aacctcgttt tgccacaagg cgtaaagttc
     1621 ggggctgttc atcacgtcgg cggcgatata cgcgccgtgc gcgggcgggc tggagtagat
     1681 gcggcggacg gtgaatttga gctgtccgaa caccaaatcc gcttcttcct tattcgggca
     1741 aaccacgctt aagccgccga cgcgctcgcc gtagagcgac aggttttttg agaaggaatt
     1801 gctgacgaac aagggcaatt ccatttccac cgctttgcgg acggcgtagg catcgctgtc
     1861 caaatcgccg ccgaagcctt ggtaggcaat gtccataaac ggaatcagtt tgcgcgtttt
     1921 gatgatgtgc aacacttcgt cccattgttg ttccgacata tccacgccgg tcgggttgtg
     1981 gcagcaggga tggaggatca ggacgctgtt ttcgggcagg gtgttgaaaa acgcggtcat
     2041 ttcgtcgaat ttcacgccga cagtggcagg gtcgtaatat gggtaagtgc cgacctcgaa
     2101 acctgcgcct tcaaaaatgc cgcgatggtt gtcccaagtc gggtcgctga cgtaggcgcg
     2161 cgcttcggga aaccagcggt gcaggaagtc cgccccgact ttgagtgcgc ccgagccgcc
     2221 caaggtttgc acggtaacga tgcgtccttg cacaagcgcg cggttgtctt tgccgaacaa
     2281 taaatgctgt accgcgctgc ggtaagtgtc caagccttcc atcggcaggt agggcgacgg
     2341 cgcaggcgcg gcggcacggg cggtttcggc gcggctcacg gattccaaca cgggcatttt
     2401 gccttcgtcg tcgaaataaa tgcctatgct caaattgact ttttcggggc gcgggtcgtt
     2461 tttgaaggtt tcgaccaaac tcaaaatcgg gtcgccggga tagtattcga tgtgtcggta
     2521 catagtcctt accttttact ttttcaaagg attttctttt tcaacaatac accactttcg
     2581 atatggtgcg taaacgggaa ttggtcgaac agggcggcac gttcgaccgc atgggtttcc
     2641 gccaaggtgt ccaaattggc acgcagcgtt tcgggattgc aggaaatgta gatgatgttg
     2701 tcaaactgcg acaccagctt caaagtttcc tcatcgatac cggcacgcgg cggatcgacg
     2761 aaaatagtgg aaaatgcgta atccgtcaaa gcaataccgc catccttaag gcgtttaaac
     2821 tcacgttttc cggtataggc ttcggtaaat tcttcagcag acagacgggc gattttgatg
     2881 ttgccgatgc ggttggcttc gatattccat tgcgccgcgc tgacggaggt tttggagatt
     2941 tcggttgcca aaacacgttc aaacttttca gataaaggaa gcgtaaagtt gccgtttccg
     3001 caatacagtt cgagcaaatc cccatccaat ccttctgctg caccgcaggc ccattcaagc
     3061 attttctgac acacggcggc attcggttgg gtaaaactgc cttcaatttg ccgataacgg
     3121 aaatcccggt tgccgacctt caaagtttcc gttacatagt cctgttttaa gactattttc
     3181 tgtcccctgc tccgcccgat gacggaaata tccaactgtt gctgtaacgc ttgcgccgcc
     3241 tgcatccact cattatcaag ccttttgtgg taaatcatgg taaccagcat ttccccgctg
     3301 agcgtagaca aaaattcgac ggcataccaa cggtttctca gttcggcaga ttgggcggca
     3361 acggcaatca attctggcat caggcggttg acggcttcgg aagccgcttc aaaacggtcg
     3421 caacggatca ggcttgcccc gccagccttt tgtccttttt caaacatggc ataaaacatt
     3481 tcaccgcctt cgtgccaaat acggaactcg gcacgcatac ggtaatgttt gtccggagat
     3541 tcgtacactt cccactcagg aacatccaaa cctgcaaaaa gggttttaag gtggtctttt
     3601 ttatcttgaa gctgctgcgt gtagtcgctc atatcgctat cctgaaaaat cagaccggat
     3661 tataagccga ttcactgcaa aacacgcaac ccctttaccg gcatataacc cgttttcctc
     3721 caacttttat gctaaaatcc gccgtctcca acataccgcc gacagaagga aaatatggca
     3781 ggcaacactt tcggacaact cttcaccgtg accacgttcg gcgaaagcca cggcgcaggt
     3841 ttgggctgca tcatcgacgg ctgcccgccc ggactggcat taaccgaatc agacatccaa
     3901 gccgacctcg accggcgcaa acccggcacc agccgccacg ttacccaacg ccgcgaagcc
     3961 gaccaaatcg aaatcctctc cggcgtattc gaaggcaaaa ccaccggcac gcccatcgcc
     4021 ctcttaatcc gcaataccga ccagcgcagc aaagactacg gcaacatcgc ccaaagtttc
     4081 cgccccggcc atgccgacta tacctattgg cacaaatacg gcacgcgcga ctaccgcggc
     4141 ggcggcagga gctccgcccg tgaaaccgcc gcccgcgttg ctgccggagc cgttgccaaa
     4201 aaatggttga aagaaaaatt cggcacggaa atcaccgcct acgttaccca agtcggcgaa
     4261 aaagaaatcc ggtttgaagg ctgcgaacac atttcccaaa atcctttttt tgccgccaac
     4321 catagccaaa ttgccgagct ggaaaactat atggacagcg tgcgcaaatc cttggattcc
     4381 gtcggcgcga agctgcatat tgaagccgcc aacgtccccg tcggcttggg cgaacccgta
     4441 ttcgaccgcc tcgatgccga aatcgcctac gccatgatgg gcatcaacgc cgtcaaaggc
     4501 gtggaaatcg gcgcaggttt tgacagcgta acgcaacgcg gcagcgagca tggcgacgag
     4561 ctcacgcctc aaggcttcct atccaaccac tcaggcggca tcttaggcgg catcagcacc
     4621 gggcaagaca tctgcgtcaa tatcgccatc aaacccacca gctccatcgc caccccgcgc
     4681 cgcagtatcg acatcaacgg caaccccatc gaactcgcca cgcacggcag acacgatccc
     4741 tgcgtcggac tgcgcgccgc gccgattgca gaagccatgc tcgcgttagt cctcatcgac
     4801 cacgccctgc gccatcgcgc gcaaaatgcg gatgttgccg tggatacgcc cgatatcgcc
     4861 cgctcggaca agtaaaaaga tttagccaaa acacgggctt tatactaaaa taccgaccat
     4921 tgccgtgccg tctgaacgca cgattcttag aggaatacaa ccgaaatgac acaagaaacc
     4981 gctttgggtg cggcactgaa atccgccgtc caaactatga gcaaaaagaa acagaccgaa
     5041 atgatcgccg accacatcta cggcaaatac gatgtgttca aacgcttcaa accgttggca
     5101 ctcggcatcg atcaggattt gattgccgct ttgccgcaat acgattccgc actgattgca
     5161 cgcgtcctcg ccaaccactg ccgacgtccg cgctatctga aagccttggc gcgcggcggc
     5221 aaacgtttcg atttgaacaa ccgtttcaaa ggcgaagtta cccccgaaga acaggcgatc
     5281 gcgcaaaacc atcctttcgt gcagcaggct ttgcaacagc agtccgccca agctgtcgcc
     5341 gaaacgccgt ctgttgaagc cgaagcagcc gaatcttccg cagcagaata aatccccaaa
     5401 cgaaatgccg tctgaaaacc gatttggttt cagacggcat tttttcgtat gcggcaatca
     5461 cggttcaaat atcgagttcc gccgtatcgc cttcgcgttc catccaggca cggcgggcgg
     5521 cggcttcgcc tttgcccatc agtttgacga agatgtcgcg cgtctcgtca tccgcgcctt
     5581 cggggatttg cacctgcaac aggcggcggg tgtcagggtg catggtggtg tctttgagct
     5641 ggtcggggtt catttcgccc aagcccttga aacggctgat ggaataggcg gtttctttga
     5701 cgccttcttt ttgcagccgc tccaaaatgc tgtcgagttc gttttggtcg agggcgtaga
     5761 atttgcgggc aggtttgctt ttgccttgtg cgttgacatc gacgcggaac agcggcggct
     5821 gggcgacgta gatgtgtccg tcggcaacca gtttcgggaa gtggcggtag aataaggtca
     5881 gcagcaaaac ttgaatatgc gagccgtcca cgtcggcatc ggacaggatg gcgattttgc
     5941 cgtagcgcag gccgcttaaa tcgggatggt cgttaatacc gtgcggatcg acgccgatgg
     6001 cgacggaaat gtcgtggatt tcggcgttgc cgaagagttg gtcggggtgg acttcaaagc
     6061 tgttgagcac tttgccgcgc aggggcagga tggcttgggt ggctttgtcg cgggcgagtt
     6121 tggcggagcc gccggcggaa tcgccttcga caaggaagag ttcgttttcg cggatgtctt
     6181 cgctttcgca gtcggtcagc ttaccgggca ggacggcgac gccgctgcct tttttctttt
     6241 caatcttttt aaccgaacgc atccgcgcct gtgcctgacg gatagcgagt tcggcgattt
     6301 ttttgccgaa gtccacattt tggtttagcc acaattccaa aggatcgccc gatacagtgg
     6361 cgacaagttt cagcgcgtcg cggttggtca gcttgtcttt ggtctgacct tggaactgcg
     6421 ggtcgaggac acgggcagag aggacaaagg cggttttgcc gaacacgtcg tcgctttgta
     6481 ctttcacgcc gcgcggcaag aggttgtgca gattgatgaa attgttgacg gcgttgaaca
     6541 cggcttgttt caagcctgct tcatgcgtgc cgcccaaagg ggtggggatg aggttgacat
     6601 agctttcgtt ggcacacgag ccttcttcca gccaagtcag ggcaaacgcc gctccttcgc
     6661 cgatgctgaa atcgccgttg tgttcgtctg aaatgtagtt ttcgcaagag aacagcggca
     6721 cagcttcttg cgcgtcggca atcaggtcgg tcagatagct tttcaggccg tcggggtagt
     6781 gccaggtttg ggtgtgcgct tcgtcttcgc ctttgaccgg acgggtcagg gaaacgcgca
     6841 cacccggcag cagcacggct ttggcacgca gcaggcgttc gagttcggga atgctgtaat
     6901 tcgggctttc aaaatatttg ccgtccggcc agacgcgcac ttcggtgccg ctgtctttga
     6961 cggcgcattt gcccacttgt gccaacggtt cgaccacgtc gccgccggca aacacgatac
     7021 ggtggatttt gccttcacgt ttgaccgtta cctcaaggcg ggtggagagg gcgttggtga
     7081 cggatacgcc cacgccgtgc agtccgcctg aaaaggcata cgcgctgccg ccgtcttttt
     7141 tgttgaactt gccgcccgcg tgcagacggg tgaacacgag ttcgactacg ggtacgcctt
     7201 cttcagggtg caggccgacg ggaataccgc ggccgttatc acggacggaa agcgaaccgt
     7261 cttcatgaat ttgcacgtcg attgcagtcg cgaaaccacc caacgcctcg tccgccgcat
     7321 tatcaatcac ttcctgacag atgtgggtcg ggctgtcggt gcgggtgtac atgccgggac
     7381 gttctttgac cggctccaag cctttgagga cggtgatgct ggattcgctg tattggttgt
     7441 ttttagccat aggaataatc tgaaagtaag aaaaacaacg ctttcagacg gcctgaaagc
     7501 gttgcgttcc gttgttttac cggttgccgg aaggttggcg ggcggcaaag tcttcataac
     7561 tttccatacc gcgcaggaag cgggaagaga gttctttcaa cgcccccaaa taaacgtcgc
     7621 gtttgaactc gatgacttcg tcaacgggcg cccaatattg atgccaacgc cagccgtcaa
     7681 attcggggtg gcgggtggcg cgcaggttga catcgcaatc ccggccggtc aggcgcagga
     7741 gataccaaat ctgcttctgc ccgcgataag agccgcgcca ttcgcggcgc acccagttgt
     7801 tcggcacgtc gtaacgcagc cagtcgcgcg tccgcccgac gattttgacg tgttgcggca
     7861 gaagtccgac ttcttcgtaa agctcgcggt acatcgcggt ttcggggctt tcgcccggct
     7921 tgatgccgcc ttgcggaaac tgccaagaat gttcgcgcac gcgcttaccc caaaagactt
     7981 cgtcgcggtt gttggttaag atgataccga cattggggcg atagccttct ctgtccaaca
     8041 cggtgtcgcc ctccgttaaa ttcaatcttg ggattttccc acaaatcgaa cggttttgac
     8101 aaatcagacg gtatggttca agcatgtcga aacacagacg gactcgggaa aatatcttaa
     8161 atttagttta caataatacg tttcaaatta ttcgggaatc agaccatgtt agatatccaa
     8221 ttgctccgca gcaacaccgc cgccgttgcc gaacggcttg cacggcgcgg ttatgacttt
     8281 gataccgcac gttttgacgc actggaagaa cgacgcaagt ccgttcaggt gaaaactgaa
     8341 gaactacaag cctcgcgcaa cagcatttcc aaacaaatcg gcgcactgaa aggtcagggc
     8401 aaacacgaag aagcgcaggc ggcgatggat caagtcgccc aaatcaaaac cgatttggaa
     8461 caggctgccg ccgatttgga tgccgttcaa aaagaattgg acgcatggtt gttgagcata
     8521 cctaacctgc cgcacgaaag cgtacctgcc ggtaaagacg aaaccgaaaa cgtcgaagtc
     8581 cgcaaagtcg gcaccccgcg cgaatttgac tttgaaatca aagaccatgt cgatttgggc
     8641 gaacctttgg gtttggattt cgaaggcggc gcaaaactct ccggcgcacg atttaccgtt
     8701 atgcgcggac aaatcgcccg tctgcaccgc gcgctggcac aattcatgct cgatacgcac
     8761 acactgaaac acggctacac cgagcattac acgccctata ttgtggacga caccaccctg
     8821 caaggtacgg gccagctgcc gaagtttgcg gaagatttgt tccacgttac ccgtggcggc
     8881 gacgagagca aaaccacgca atatctgatt ccgacagctg aagttaccct gaccaatacc
     8941 gttgcaggca gcattttaga cgaaggcgga ctgccgctga aactgaccgc ccactcgccc
     9001 tgcttccgca gcgaggcggg ttcgtacggc aaagacacgc gcggtctgat tcgccagcac
     9061 cagttcgaca aagtggaaat ggttcaaatc gttcatcccg aaaaatcata cgaaacgctg
     9121 gaagaaatgg tcggccatgc cgaaaacatc ctgaaggctt tggaactgcc ctaccgcgtg
     9181 attaccctgt gtaccggcga catgggcttc ggcgcaacca aaacgtatga cttggaagtt
     9241 tgggtaccgg cgcaaaacac ctaccgcgaa atctcaagct gctccaactg cgaagatttc
     9301 caagcccgcc gcatgaaggc gcgtttcaaa gacgaaaacg gcaaaaaccg cttggtacat
     9361 actttgaacg gctccggctt ggctgtcggc agaacgctgg tcgccgtatt ggaaaaccat
     9421 caaaacgccg acggcagcat caacatccct gccgcactgc aaccgtatat gggcggcgtt
     9481 gccaagttgg aagtgaaata agtttgcaag ctgcctgaac gtcaaatgcc gtctgaaacc
     9541 tggttcagac ggcatttcct ttaaactttt aaaacacatc aaccgtcggc acgaaccgca
     9601 ttgccgcaat cgccggtctg tccgacctcg cggatattgg acagcgtaac ttccgaaata
     9661 ttacccaacg cctcttccgt caaaaatgcc tgatggccgg taaacagcac attatgacaa
     9721 gacgacaggc ggcggaacac gtcgtcggta atcacatcgt tggatttgtc ttcaaaaaac
     9781 aattcgcgtt cgttctcgta cacgtccata cccaatgcgc cgattttccg gcgtttcaac
     9841 gcctcaatcg cggcagaact gtcaatcaga ccgccacggc tggtgttgat aatcatcacg
     9901 ccgtctttca ttttgtcgaa cgccgcttcg ttcagcatat agtggttttc cggcgtggag
     9961 gggcaatgca gcgtgatgat gtccgaccgg gcatacagct cgttcaactc cacatacctg
    10021 ccgccgattt tttccgcttc ggggttgcaa aacggatcgt aagccagcag gttcatgccg
    10081 aaacccttta aaatccgcat ggttgcaata ccgattttcc ccgtgccgat aacgcccgcc
    10141 gttttgccgt acatattgaa accggtcaga ccttccagcg aaaaattcgc atcgcgggta
    10201 cgctgatagg ctttgtggat acggcggttc aacgtcagca tcagaccgac cgtatgttcc
    10261 gcaaccgatt cgggcgaata ggcaggcacg cgcacgactt tcaagcccaa ctcttcagcc
    10321 gcctttaaat ccacattatt gaagccggca caacgcaacg ccacagtttt cacgccaatt
    10381 tgcgccaatt tttccaacac gggccggctg ccgtcgtcgt tcacaaaaat acagaccgcc
    10441 tccgcacctt ccgccatttt cgccgttttc gcatccagca taaaatcaaa aaactccagc
    10501 tcgaagccga aatgccggtt ggcgcgggta aaatgttcgc ggtcatagct tttcgtaccg
    10561 taaatcgcaa tcttcatcaa tatgtccagt tgtcgtctat ggttgagaaa cggcataata
    10621 cactgaattc aaacaaatca gtagaatatg gtggattaaa attgattgca tgcacggcat
    10681 ttccatttca aaacacaaaa ctcaatcgcc cattgccgcc agaagctccg cctgatgctc
    10741 ggcaatcagg gcatcggtga tttctgccaa atcgccgtcc atcacaaaat ccagcttgtg
    10801 cagggtgagg ttgatgcggt ggtctgttac cctgccttgg ggatagttgt aggtgcggat
    10861 gcgttcgctg cggtctccgc tgccgatgag ggatttgcgt tcggcggctt ctttggcttg
    10921 ggcttcgcgt ttttgcgcgt cgttcaggcg ggcggcgagg actttcatcg cctgcgcttt
    10981 gttggcgtgt tggctgcgtc cgtcttggca ttcgaccacc atgcctgtgg gcaggtgggt
    11041 gatgcggacg gcggaatcgg ttttattgat gtgctgcccg cccgcgccgg atgcgcgaaa
    11101 ggtgtcgatg cgaaggtcga cggggttcaa ctcgatgtct tccagttcgt ccgcttcggg
    11161 catgacggca acggtgcagg cggaggtgtg gatgcggcct tggctttcgg tggcggggac
    11221 gcgctgcacg cggtgtccgc ccgattcaaa tttcaaacgg ctgtacgcgc ctaagccgat
    11281 aatacgggcg ataacttctt tatagccgcc caactcgctt tcgttggcgg acacgatttc
    11341 aacctgccag cggttgcgtt cggcgtagcg gctgtacata cgcagcaaat cgccagcaaa
    11401 cagcgcggct tcgtcgccgc ccgttccggc gcgtacttcg ataaagatgt ttttgtcgtc
    11461 gtcggcatct ttgggcagca gcagtttttg caattcggta tcgagtgcgc cgattttggc
    11521 tttcgccgct tcgatttctt cggcggcaaa gtctttcatt tcggggtcgg acaacatttc
    11581 ttcggcatcc gccaagtcgc tttgggcaag ccgatagttt tggaacactt cgacgacggg
    11641 ggtcagttca gcgtgttcgc gcgtgagctt gcggtagttg tccatatcgg acgtggcttc
    11701 gggttgccct aaaaggtggg taacttcttc caaccggtcg ccgagttgtt gcagtttttc
    11761 taagatagac ggcttcataa ttcttccata acaaacgccg cctgaatttc agacggcatc
    11821 aacactgaat tattataata ggttttccgg acattcaaaa agataatctc agatggataa
    11881 cctaccgtcc caacagggca tcggcattgc gctccgttac cttggcaatc tcttccacac
    11941 aggttccgcg gatttccgca gcaatctttg caatacccgg aatattggca ggcgtattaa
    12001 tctctttttt cagcataaac gggctgtcgg tttccaatac gaaatccccg tcgttcaagg
    12061 ctttaagcgt atcgcgcact ttacgcgcgt tcggattgag caacaacgaa ccgatgccga
    12121 ttttgaaacc cagtttcgtc aacacgcgcg cttcttccgc gctgcctgag aaggcgtgaa
    12181 cgatgccgcc ttgggcaaag cctgtctgtt tgacggcggc ggcgatgtcg gcggtggctt
    12241 tcagattatg gataatcacg cggcggcgca gggtttgcgc gatttcaagc tggcggacga
    12301 aaacttggat ttgccgttcg cgctgcggcg gcgtttgggt tttgtcgtaa aaatccaagc
    12361 cgatttcgcc gacccatgct gtcggatgcg ctttcaatgt ttgttccaaa agctggaaat
    12421 ccggttcgga aacgtcgtct gaaaaccaag gatggatgcc gagcgcgata tggatattgc
    12481 cccaaagcgg attttcagac aacctttgag ccaaatccgc cacgtcctgc caatcctgcg
    12541 gccgagtcgc gggaacgata aaccgcccta cccctgcctc ccgcgctgcg gtcaggatgt
    12601 gcggcaggtt ttcgcgcagg gcggcatcgg caaggtggca gtgggtgtcg gtgaagttca
    12661 tttcgattcc gcaatgcggc aggttcacat aaaaataagg cagggcggca agggatgcag
    12721 tgtacgggcg gcgcataagg cgcggtcaac gccccgtcat tgccatttca atccgctata
    12781 acttcagtct tgccaactct ttttccacct cttccttacc ccagccttgc gatacggcga
    12841 gggtcataag cgcggtggcg gtttcgaggt tgcatttgcc gccgttgatg atgcctgagt
    12901 tgcggaacgc gttgccttgc gcgtaaacgg cagcggttct gccttgtcgg acttggctga
    12961 tgttgagcag cagtttgcct tgctgcgtaa agtcttgcac ggcgcggatg aaaccttcgt
    13021 ctgcgggcgt gttgccgtgt ccgtaacttt gcaggataag ggcgtgggcg ggaagctgtc
    13081 cgagtccgtc ggcaagttct tggacggcaa agccggggat aagcgtgcgg acggcgattt
    13141 ttgcctgcgg gtcgggataa cggattttga ggccgtctga aacatctgcc gcgtcctgat
    13201 tcgggatgcg gacattgtgc cagccctggg tttcgtccca ctccgccagt gcgccgaaat
    13261 gcggattgtc gaaaccggcg gcggtttcgg tgctgacttt gctgctgccg acggcgggat
    13321 aaagtttgcc gtcaaaggca ataagcgtct gcttcaatcc gagtgggaat gcggcaacgg
    13381 cagtcgccag attgcgcggc gcgtcgctgc ccacggcgtt gtacggccat tgggagccgg
    13441 tcaggacgat tggcttgtcc aaaccctgca gggcgagggc gaacagattg gcggtatagg
    13501 cgagcgtgtc cgtgccgtgc agcaccaaaa cgccgtcgta ctgcggcagc gcggcggcaa
    13561 tgatgtccag ccaagcgcac cagttttgca gttggacggc ggaagaatca atcagcgtcc
    13621 cacaaacgtg ccaatcgaaa tccatgccgt ttgaaaacgg cgcgagtgcc tgcccgacca
    13681 gcgcggtatc ggggcgcagg ccttcgctgc tttgcgtcat gccgatggtg ccgccggtat
    13741 aaaggacaaa aattttctgt ttcatggctc atcctgaaaa tgcaaagacc gcctgaaacg
    13801 cgaaatgcgt ccggacggtc gaaaatctga tttactgctg cttctgtgcc gcttttttcg
    13861 ctgccttttc cgccttgcgt ttggcgcgtt tttcgctcaa ttgtgcgcgg taaagctgat
    13921 aatgtcggcg tttgcgccac cagaaccacg ccagcgccgc cgccagcacg cccaatgcga
    13981 tgaagatgcc ggattgcagg ctgtgcattt tcgccatcag ccaatcgatg ttgtgcgcgc
    14041 cgtactcgcc caagtaaatc caaacgggca cggaaatcag cgcggcaagc ccgtccataa
    14101 tcagaaagcg cagatacgat actttgcggc tgatgccggc ggtaacgaaa acggcagtcc
    14161 gcaaacccgg caggaagcgg gcgacaaaca gaacccagtt gccgtatttg tcgaattttt
    14221 cctgaacctg tgcgtaacgt ttcggcgtca tgatgcgcgc aataggtttg aacttgagga
    14281 ttttctgccc ccagatgcgt ccggcggcga acatgatgcc gtccccgacc aatacgccga
    14341 gcataccgac tgcaaacata atatgcggat tggtataacc catacccgaa atcacgccgc
    14401 ctgttaccaa ggtcaaatcc tcgggaatcg gcacgccgaa accgcagatg accaaaacga
    14461 aaaacacggc cgcatagccg tattcgacaa aaaaggcttc caaaagggca aacatagcgg
    14521 atattccatt gtcggagata aaaagtcaga acaagccgaa acattttctg cgcgaaacgg
    14581 cattctatca aagattatgc cgtctgaaag cggcaagggg cagattcgcg ccctctgccg
    14641 gacgttcaga cggcatttaa acaggaaaac cgccgctgtt gaaacggttt ttcctgattt
    14701 tgcctaaatg ttgccgatgg cggaagcaat acgttccgcc ccttcgcgcg cccaatcggc
    14761 ctgtcgtgcc tcaaccatca ctcgcacgac gggttcggtt cccgaagcgc gcaacacaac
    14821 ccttcctttg ccttcgagtt ctttttccac ttcggccaac acgtctttcg aagcctcctg
    14881 ccatttctga cccttttgga tgcgcacgtt aatcatagtt tgcggatacg gctgccagtc
    14941 ggcgcaaacg gtggcgaggt cttggttcag gatttgcagc gccgccaaaa cttgcagcgc
    15001 ggaaataatg ccgtcgccgg tattgtgttt gtccatgcac aaaatatggc cgctggcttc
    15061 gccgccgatg agccagccgc gttggttcag ctgttccaac acatagcggt cgccgacttt
    15121 ggctcggcag aaatcgacgc cctgctcttt cagggcgatt tccatcgcca tattggtcat
    15181 gaccgtgccg accacgccgc cgatgttgat accttctcgg gctcgggctt tggcgatgac
    15241 gtaaatcagg ctgtcgccgt cgtaaacctt accgttttta tcgaccatca tcaggcggtc
    15301 gccgtcgccg tctaaggcga tgccgtagtc ggcttcatgc tgtaaaacgg ctgcctggag
    15361 tgtcttggta taagtcgcac cgcatttttc attgatattg tagccgttgg gttcgtcgcc
    15421 gatgctgacg acctgtgcgc ccagttcgtg aaacaccttg ggtgcgacac cgtaccccgc
    15481 gccgttggcg gtatcgacaa ccaacttcaa accccgaagg tcggaatggc tggggaaggt
    15541 ggatttgcaa aattcgatat agcggtcgtc cgcaccgctg atgcggcgtg cgcgaccgag
    15601 gcgtgcagaa ggctgggttt tcatttcttc gtcgattttg gcttcgattt ccaactcgat
    15661 ttcatcggaa agcttcacac caccctcggc aaagaatttg atgccgttgt cggaataggc
    15721 attgtgcgac gcggaaatca tcacgccggc ggacaaacgc agcgcacggg tcagataagc
    15781 cacgccgggc gtaggcagcg gaccggtttg gataacgttg acacctgcgg cggtaaaacc
    15841 tgccaccaaa gcggcttcca gcatataacc ggaaatacgc gtatccttgc cgattaaaac
    15901 ggtcggtttc tggtcggtgt cgtgctgaac caaaacctgc cccgccgcat agccgagttt
    15961 caatacaaaa tcgggcgtaa tcggaaactg cccgacttcg ccgcgcacgc cgtccgtgcc
    16021 gaaatatttt tttgccatgt gttgctccga gaatgtgaac cgttgtccga gattatacag
    16081 tcagtttgtg ccttgctgtc tgcaccgttg atgccgtctg aagccgcccc gcccttttca
    16141 gacggcataa agtatgcgaa ccgctgttta cagattgatg cccaacgctt cccacacctt
    16201 caacgcatcc gccgtcgcct tcacatcatg cacccgcacg atttgcgcgc cgcgcgccac
    16261 ggaagccagc gccgccgcca cgctgccgtg tacgcgcgcc gccgcgtctg cctcgccggt
    16321 cagctcgcct atcgtgctct tgcgcgacac gccgaccagc agcggcaaac cggtttccgc
    16381 catcaattcg ggcaaatgcc gcatcagcgc gatattgtgt tgcaaggttt tgccgaagcc
    16441 ggagccgaaa ccggggtcga gtgtgatgcg ttgcggtgcg atgcctgccg cgacacattc
    16501 cgccgcccgc gccttcaaat accttgccac ttcgccgaca acatcctgat atttcggatt
    16561 aatctgcatg ttttccggca aaccctgcat gtgcatcagg caaatgcccg tgtccgtctg
    16621 gcgcgccagc aattcgaccg cgccttcgtc ggtcaacgcc gccacatcgt taataatatc
    16681 gatgccgccg agtgccaacg ctttttccat aaccaccgtg cggcgcgtgt ccaaactgac
    16741 gggaacgccc caccccgcca cttctgccaa tacaggttca acccgcgccc attcttcttc
    16801 aggcgaaaca taatccgcac ctgcccgcgt cgattcaccg ccgatgtcga gaatgtccgc
    16861 accctctttc aaaagccgtt cggcatgcgc caaagcggtt tgggcgtttt gcgaatacgc
    16921 gccgccgtcg gaaaaagaat cgggcgtgag attcacgata cccatgattt tcggtttgtc
    16981 caaaccgatt tcaaaccgtc ctgcctgcca aacgtgtcgt gccatctgaa ctcctcccaa
    17041 aataaaaaac agattatatg ccgtctgaaa ccgtcttgtg cgcttcagac ggcaccgcta
    17101 ttcgggcggc agacggcatg ttgtccgaat gtctgctccg cctttgaatc tgccggtatg
    17161 cctgctatcc gcccgccttt tcaaaacagg ttccgacgat tccgcacgcg cctgccgcct
    17221 ttgccaagcc gtacaggatt tcctgcggca tatcgcggtt ccataatccc gtaatattcg
    17281 caatcacggg cagatggctg atttggcgga ctttcacgat ggattcgaca tccaaacggt
    17341 agggatggtc tttggtatgg ctcaacacgc ccgactcgcc cagaatcagg tggcggttgc
    17401 cgcgcgagac gacatattct gcggcattca accaatcttc ggcagaatgg tgcttgtctt
    17461 tacacagcac caagggaatg ttcaggcttc cggcttcatt caatacggca agatcggaca
    17521 tcagcccgcc gcccaaatac aggatgtccg cccccgcatt caaagccgct tcgacatggc
    17581 ggacgttgcg gacgcgcacc aatacgggtt tccctgcgcc atgcgccgat gcggtctgtt
    17641 ccaccaaccg tctgcaccgt ccccgccctt catccgcact tgaagtgtcg tataagtttg
    17701 ccgaagtgaa aaacggatcc agaaacactg catccgcatt gcgccatact gacggttctg
    17761 cggcgatacg gacggtttcc ccgccgccga aagccacgcc tttggcggca acgcggctgt
    17821 cttccgcccg attttcccga ctgacggttt tccatgtatc caaaatgcgg acggctttct
    17881 cgacctccgg cagcgtctgc acctccctga cgctcaaaac cctatcgtcg ccgattgcgc
    17941 cgatgacagt acgctcgtcg ccgtgagaaa tgtgttctcg cagacctctg ctgcggataa
    18001 aggcgacaac gccggcaatg tccgcttcgg cggcacgcct gctcatgaca ataatcatat
    18061 ttcctcctga cacaagaaac ggcctgccca aaataggatt tttgcaagcc gtgttatact
    18121 gtggcgtgtt ttacagattg ttcgggctat ggatttatta tcggttttcc acaaataccg
    18181 tctgaaatat gcggtggccg tgctgacgat actgcttttg gcggcagtcg ggctgcacgc
    18241 ttccgtatat cgcaccttca cgcctgaaaa catccgcagc cgcctccaac aaagcattgc
    18301 acacacacac cggaaaatct cgtttgatgc ggacattcag cgcaggctcc tgccccggcc
    18361 gaccgtcatc ctgaaaaacc tgaccattac cgaacccggc ggcgaccaga ctgccgtttc
    18421 cgtccaagaa accaaaatcg gattgagctg gaaaaacctg tggtcggatc agatacagat
    18481 tgaaaaatgg gtggtttcga gtgcggaact tgccctgacg cgtgacggga aaggtgtttg
    18541 gaacatccaa gacctgatcg acagccaaaa acgccaagcc tcagtcaacc gcattatcgt
    18601 cgaaaacagc accgtccgcc tcaatttcct gcaggaacag cttatcctga aggaaatcaa
    18661 cctcaacctg caatcccccg attcgtcggg gcagccgttt gaaagttcgg gcatactggt
    18721 ttggggaaag ctgtccgtcc cgtggaaaag cagggggctg ttcctttcag acggcatcgg
    18781 cacgcccaaa atctcaccgt tccattttga agcttccact tcgctggacg gacacggcat
    18841 caccatttcc accaccggca cacccgccat ccgtttcaac gccggcggag cggatgccgc
    18901 cggcctcggc ctgcgtgcag acacttcctt ccgcaacctc cacctgaccg cgcaaatccc
    18961 cgcgctggca ctcaggaaca acagcattaa aattgaaacc gtcaacggcg catttaccgc
    19021 cggcggcgaa tatgcccaat gggacggttc gttcaaactc gacaaagcca acctgcactc
    19081 cggcatcgcc aacatcggca acgccgaaat ctccggcagc ttcaaaacac cgcgccacca
    19141 gaccaacttc tccctcaatt cgccgctcgt atggacggaa aacaaagggc tggacgcgcc
    19201 gcgcctgtat gtatcgaccc ttcaggatac cgtcaaccgc ctgccgcaac cccgtttcat
    19261 cagccggctc gacggttcgc tgtccgtacc gaatctgcaa aattggaatg ccgaattaaa
    19321 cggcacattc gaccgccaaa ccgttgccgc gaaattcaga tacacacatg aagacgcacc
    19381 gcatctggaa gccgccgtcg cactgcaaaa attgaacctg accccctatc ttgacgacgt
    19441 gcggcaacaa aacggcaaaa tatttcccga caccctcgcc aagctgtccg gcgacatcga
    19501 ggcgcacctg aaaatcggaa aagtccaact tcccggcctg caactggacg atatggaaac
    19561 ctacctccac gccgacaaag accatatcgc gctcagccgt ttcaagtcag ggctttacgg
    19621 cggccatacc gaaggcggca tcagcatcgc caacacccgt cccgccactt accgcctgca
    19681 acagaatgca agcaacatcc aaatccaacc gctgctgcaa gacctgttcg gcttccacag
    19741 cttcagcggc aacggcgacg cggtcatcga cctgaccgcg ggcggcgaaa cccgaaaaga
    19801 gcttatccgc tcgcttcagg gcagcctgtc gctaaatatt tccaacggtg cgtggcacgg
    19861 tatcgacatg gacaatatcc tgaaaaacgg catttcgggc aaaactgccg acaatgccgc
    19921 acccagcaca cccttccacc gattcacgct caacagcgaa atttcagacg gcatcagccg
    19981 ccacatcgat accgaactct tctccgacag cctctatgtt accagcaacg gctataccaa
    20041 tctggatacg caggaattgt ctgaagatgt ccttatccgc aacgccgtcc atccgaaaaa
    20101 caaaccgatt cccctgaaaa tcaccggtac agtggacaaa ccgtccatta ccgtcgatta
    20161 cggcaggctg accggcggca tcaattcgcg caaagagaaa cagaaaatcc tcgaagacac
    20221 cctgctggaa caatggcagt ggctcaaacc taaagaaccg taaacaccct gcgtacaaaa
    20281 atgccgtctg aaacacatta agcttcagac ggcattaccg taaaacctac aaccccaatt
    20341 ccccccaaat cccatcaatc ttagccgtaa cggcagggtc ttttttgatt acacgccccc
    20401 attcgcgttc ggtttcgccc ggccacttat tggttgcatc caaacccatt ttgccgccga
    20461 gtccgctgac ggggctggcg aagtcgaggt agtcgatggg cgtgttttct accaaaacag
    20521 tgtcgcgcac ggggtccatg cgcgtggtga ccgcccagat gacttctttc cagtcgcgca
    20581 cgttcacatc gtcatccacc acgataatga atttggtata cataaactgg cgcaggaacg
    20641 accagcagcc catcatcacg cgcttggcgt gtccggcgta ctgttttttc atgccgatca
    20701 ccgccatgcg gtaggaacag ccttcgggcg gcaggtagaa atcggtgatt tcggggaact
    20761 gcttttgcaa aagcggtacg aacacttcgt tcaatgccac acccaaaacg gcaggctcat
    20821 cgggcggttt gcccgtgtag gtcgaatggt aaatcgggtt ttcgcgcatg gtgatgcgtt
    20881 cgaccgtaaa cacagggaaa tgatcctgct cgttgtaata gccggtgtgg tcgccgtacg
    20941 ggccttccaa cgcggtttcg ttcggatgga tgacgccttc caacacgatt tctgcgcggg
    21001 caggcacttg caaatcgttg ccgatacatt tcaccagctc cgtccgcgaa ccgcgcagca
    21061 gtccggcaaa ctggtattcg ctcaaggtat cgggaacagg cgttaccgcg cccaaaatgg
    21121 tggcggggtc gcagccgagt acgacggcga cgggatacgg cgtatcggga ttgagtttgc
    21181 ggaattcctg ataatccaac gcgccgccgc gatgcgacag ccagcgcata atcagcttgt
    21241 ttttgccgat gagttgttgg cggtaaatgc cgagattttg gcgttttttg tgcggcccgc
    21301 gcgttacggt caagccccat gttaccagcg gcgcaacgtc ttccggccag caatgctgaa
    21361 tcggaagttt atacaaatca acgttttcgc cttcccacac gatttcctga cacggcgcgt
    21421 ttttcaccac gttcggcgcc atgctccaaa tgtctttcag tagcggcagt ttggaaaacg
    21481 catctttgat gcctttgggc ggttcgggtt ctttcaaata cgccagcgtc tgcccgattt
    21541 cacgcagctt ggacacgctg tccgcgccca tgcccatcgc cacacgttcg ggcgtgccga
    21601 acaggtttgc caacacagga taaccgtagc gcgtaccgtc gggcttaacc gggttttcaa
    21661 acagcaacgc cggcccttcg gcgcgcagca cgcggtcggc gatttcggtc atttccaaat
    21721 gcggggaaac ggggtgcgcg atgcgtttga gtttgccctg ctgctcgagc atggcgatga
    21781 agtcgcgcag gtctttgtat ttcatattca tcctttttgt ccttttatcc tgagcaatcc
    21841 gattcggata ccgcccctat ccttgcctgc gcttcggcat attctatgcc gtgataaaag
    21901 tcgcgtacca gcggatgttc gctgccttga tggagttgca acaaaggacg ttgaccatcg
    21961 ggttgggtaa cgacattgca atgcaaaccg aaggtgtcgg tttcataagg gggcagccgg
    22021 ttgcagatca tcccgaaata aacgacattt tccggattgt cgtgaaagtg gctttgatag
    22081 tcgttaaaac tcttttcgct gacggacacc cacacgccgt attccagcgt ttcctcatgc
    22141 ccgataatcg gaataagcag caccgcgcgg ataaaacggt cggtttggtc gggatagcgg
    22201 atgatgcaga aatcgccgcc gcattcagct tggtaagcaa tgcgttcttc ttcactgagt
    22261 tgattatagg gatagggggc ggtaaagccg attgcgggca tatcttcatg tttttcgccg
    22321 caggaagtgc aagtgtacat aaggtttcag actttcaaaa cgagtttgcg gtaaagccat
    22381 tcgccggcaa acggcatccc catcaataca taggcaatca cgccggtata aaccgcccac
    22441 caatcatatc gccccaaccg tgccaacaaa gcggcaagcg tcccgttggc aataaagaat
    22501 acgcaccaaa cctgcgttac ccggcgggta tagcgcacag ctttttcagg caggtcgggc
    22561 tgttgcagcc gcgcaagttt ttcaatcacc gtctgcccgg caaacaggct gccgccgaac
    22621 accgccaaca tcatcagatt gacgaggacg ggataccaat acatcgaatc atgccgcccg
    22681 aacaccaata ctgcggcaaa aaacagtgta ataaacaaag ccgcataacg ctgttggggc
    22741 agtttggcag tcagggcgcg cagcagccac aacccgcaca tcgccgccgc cagccagaca
    22801 aaccagcccg cctccctgcc gtaatatagt ggattaacaa aaaccagtac agcgttgcct
    22861 cgccttgccg tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta atccactata
    22921 ccacaaagcg ggataggcaa tgcttaatac ggtcagaaaa atatgtccga aaaaaccggg
    22981 tttcattttg aatccgcaca aatgttttca gacggcatcc gataaaaaca tgccgtctga
    23041 aaaataatta gaaatacccg attagcccgc ctgaatcttc aataccgcct gtaccacgtc
    23101 gttgacggtg cggacattgc ggaaatcttc agcctgcagc ttgcgggctg ccacagccat
    23161 tcacgattgc gatatacccg gcggcattcc cgactgccgt tcagcagaaa gcgcacccat
    23221 tccaaaccgc tccaatatgc ctcgggcagc ataccggctt cagacggcat atcgtccgaa
    23281 gcagataaag tcaggctgta acgcgtccct ttggtcagaa ccatcgccaa agcataagca
    23341 aacggcgcgc gcgttgccga tacggcatat ccttccggca gcggatcgtc cgccgccaaa
    23401 accaaaaccg acccgcatcc ctcttccaac agtgatgccg cttccgccaa tgccgtttcc
    23461 acaccgtcgg cacacacggc caatgccgtc tgctcgctca tatcccgcct caatatcgac
    23521 cattaccccg ccgttgcatt gtgcaccgac aaaccgaacg aagtcggcga cacggtttgc
    23581 gatttcaaca gttccaacca caaatcgaaa ctgcgcgcca tttccccgtc gtgcgaggca
    23641 taaactatag tggattaaat ttt
//
LOCUS       DAWKOG010000011        23326 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.011, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000011 DAWKOG010000000
VERSION     DAWKOG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23326
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.011"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            258..1100
                     /locus_tag="WJK66_000361"
     CDS             258..1100
                     /locus_tag="WJK66_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEZ7578126.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     SDRVAVIATLPLTRDESWSQLAVDELVMFREGNIVRHDRPENPVYMSVEEGLETARAA
                     GVAV"
     gene            1130..1594
                     /gene="nrdR"
                     /locus_tag="WJK66_000362"
     CDS             1130..1594
                     /gene="nrdR"
                     /locus_tag="WJK66_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEZ7578127.1"
                     /translation="MKCPFCAHPDTRVADSRLMDERNAVRRRRYCPNCGKRFGTLETA
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     IPSAALADMVLKELLRQDTEAAVRFAALHKRFDNPADFASWLAQAVKTDRKA"
     gene            1626..2735
                     /gene="ribD"
                     /locus_tag="WJK66_000363"
     CDS             1626..2735
                     /gene="ribD"
                     /locus_tag="WJK66_000363"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEZ7578128.1"
                     /translation="MFSETDISMMQNALKLAALGRFSTSPNPRVGCVIAHGSQIVGQG
                     FHVKAGEPHAEVHALHQAGEMAKGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPLVAGKGLALLESAGIKTECGLLENKARELNRGFLSSIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPLDSHLLTDGQSPTYIATLERNEDRLHPYREHAHIRILMPSETAGGK
                     IDLNHLMRLLADEGFGEIMVEAGSELTSAFLAEDLADEIVLYRSPKILGSGKDLLSLP
                     ENRAALSAPPLWTPVSSEILGHDIKTVFRKNDNAF"
     gene            2930..4351
                     /locus_tag="WJK66_000364"
     CDS             2930..4351
                     /locus_tag="WJK66_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEZ7578129.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYATADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVSKLAILLLLPLT
                     VGLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRRAPFSSAVLHRGLRYGIP
                     IALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFR
                     AIEANAPPARLSATAESAAALLASALCLTGIFSPLASLLLPENYAAVRFIVVSCMLPP
                     LFCTLVEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGARGAAVACAASFW
                     LFFVFKTESSCRLWQPLKRLPLYMHTLFCLASSAAYTCFGTPANYPLFAGIWAAYLAG
                     CILRHRKDLHKLFHYLKKQGFPL"
     gene            4348..5421
                     /locus_tag="WJK66_000365"
     CDS             4348..5421
                     /locus_tag="WJK66_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ7578130.1"
                     /translation="MKIVLTTSMAGLGGTETATVRLGRLLRQHGHDIMLASSDGPLLK
                     EAQALGIRWQPVDFYRGGLFGYLKGMIAYARMLRREQPDIIDCQMARVVPACAIAAKI
                     VSPKTKIIYHSHGLDAATYPKIAKLFDKLGVYIIGNCKHEREKLIRHGFPAGRIAYAY
                     NALPPPPEFHFRKTEKECAVLGTLSRLDTVRAVHLMLDIFKKMVDRGMPVRLNVAGIG
                     EEMDNLKAQAKRLGIEDKVTFLGGIRDLTAYFKDVDILVNTPHCIGDHGAGVGNNILE
                     AGLYDTPVVTYDMAGISEMVVNGETGYCIPFGDEEAFIAAVDTLVRQPLLREAMGKRL
                     HAHVAALCSDDEIYRTTMAAYTM"
     gene            5441..6609
                     /gene="pglH"
                     /locus_tag="WJK66_000366"
                     /pseudo
     CDS             5441..6609
                     /gene="pglH"
                     /locus_tag="WJK66_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminyl-a1,3-N,
                     N'-diacetylbacillosaminyl-a1-diphospho-di-trans,
                     octa-cis-undecaprenol synthase PglH"
     gene            6602..7843
                     /locus_tag="WJK66_000367"
     CDS             6602..7843
                     /locus_tag="WJK66_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HEZ7578131.1"
                     /translation="MNKFFKRLFDIIASASGLIVLSPVFLILAYLIRKNLGSPVFFIQ
                     ERPGKGGKPFKMIKFRSMRDAVDKYGNPLPDSERLTDFGKKLRAASLDELPELWNVLK
                     GDMSLVGPRPLLMQYLPLYDNFQNRRHEVKPGITGWAQVNGRNATSWDERFACDIWYI
                     DHFSLCLDIKILLLTVKKVLIKEGISAQGEATMPPFTGKRKLAVVGAGGHGKVVAELA
                     AALGTYGEIVFLDDRVQGSVNGFPVIGTTLLLENSLSPEQFDIAVAVGNNHIRRQIAE
                     KAAALGFALPVLVHPDATVSPSATVGQGSVVMAKAVVQAGSVLKDGVIVNTAATVDHD
                     CLLDAFVHISPGAHLSGNTRIGEESWIGTGACSRQQIRIGSRATIGAGAVVVRDVSDG
                     MTVAGNPTKPLAGKNTETLRS"
     gene            7921..9096
                     /locus_tag="WJK66_000368"
     CDS             7921..9096
                     /locus_tag="WJK66_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEZ7578132.1"
                     /translation="MLNTFLSPWPCFTQEEADAVSKVLLSNKVNYWTGSECREFEKEF
                     AAFAGTRYAVALSNGTLALDAALKAMGIGAGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAVLTPNTKAIIVVHLAGMPAEMDGIMALAKEHDLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKTLWSKMWSYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAAIGRIQLKRLPEWTACRQAHAAKLAESLG
                     KFKSIRLVEVADYIGHAQYKFYAFVKPEHLKDGWTRDRIVNELNARKVPCYQGSCSEV
                     YLEKSFDNTPWRPKERLKNAVELGDTSLMFLVHPTLTDGEIAFCKEHIEAVLTEATR"
     gene            9144..11054
                     /gene="pglD"
                     /locus_tag="WJK66_000369"
     CDS             9144..11054
                     /gene="pglD"
                     /locus_tag="WJK66_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HEZ7578133.1"
                     /translation="MTLETLLALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSNEW
                     FDPANWQSFLLTAPLTIGLFVRMGLYRAVTRFISFRILSTALAGSLASAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGRSGRQLLEA
                     VKQMREYSAAAFVDDDPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPGATQEQ
                     RRRIINKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDDRLMSAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAIEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATTSGVRTFVLISTDKAVRPTNTMGASKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPLTLTHPEITRYFMTIPEAAQLVIQAGAMGTGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPNTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TAHPRIMTADEAMLPWHELSVLLDRIRSACDRYDQQAIRTLLINAPTGFAPSDGICDL
                     LWVRETHRKNAV"
     gene            11110..12402
                     /locus_tag="WJK66_000370"
     CDS             11110..12402
                     /locus_tag="WJK66_000370"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HEZ7578134.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAAEHAVENIGNYSNPQGDAALIDALTAFLNREYGWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFNLSDGTSAEKAILLPLAPEYIGYADVHVEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDSEMARLD
                     ALAQEHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTR
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(12454..13290)
                     /locus_tag="WJK66_000371"
     CDS             complement(12454..13290)
                     /locus_tag="WJK66_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEZ7578135.1"
                     /translation="MIDLHCHSTVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLAQVRQGRLKRLE
                     AIAAKLEKRGISGAYDGALALAANKEMVSRTHVAEFLIRVGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGLAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYALLAERFGMLASAGSDFHRLNDFSGGILGACPELPENCRPVWAHFS
                     RY"
     gene            13299..13541
                     /locus_tag="WJK66_000372"
     CDS             13299..13541
                     /locus_tag="WJK66_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578136.1"
                     /translation="MCVVNGGQGVSILSGGVAAVISKNKCMANAKEKPLCRHSRAGGN
                     PDFDLLEMFKVNRYFKLPDSCFRGNGDMTVFNFNLL"
     gene            complement(13583..13792)
                     /locus_tag="WJK66_000373"
     CDS             complement(13583..13792)
                     /locus_tag="WJK66_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578137.1"
                     /translation="MDTTLKYKAEALLGEPLLEEPVRPELWECCGSDCGEACIQTIYW
                     ADKARYDAQRRKLKEAGWAEDENAV"
     gene            complement(13770..14084)
                     /locus_tag="WJK66_000374"
     CDS             complement(13770..14084)
                     /locus_tag="WJK66_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ7578138.1"
                     /translation="MCCRFCGNFGYGGGRCYNNGLFLQGNIMEATVYLEDNEYIALCD
                     LLKLAGLAESGGQAKAFIAEGLVLRNGETETRKTAKIRGGEVIEFDGARLEIADGYDP
                     EV"
     gene            14542..17976
                     /gene="dnaE"
                     /locus_tag="WJK66_000375"
     CDS             14542..17976
                     /gene="dnaE"
                     /locus_tag="WJK66_000375"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEZ7578139.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKTCRGAGIKPIGAADVWIGNPNAPDKPFRAMLIIRNDAGYLRLSELLT
                     AAYVGKDRNIHHAELNPEWLENGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDAFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMSRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPGQFFIPPETMAERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSLDDYLIKLSNEGLQERMVQLYPDEAERAEKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVREKYGAEAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLGLDDAMKAEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTTGDIVDVGKIPLDDQAAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPNMKIRYALGAIKGTGEAAVESIIAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMNNADQKAANANQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPQKLSK
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRILANQVMTLQTARERYARSLSLALAPHHDIGELVQLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            18254..21502
                     /gene="cas9"
                     /locus_tag="WJK66_000376"
     CDS             18254..21502
                     /gene="cas9"
                     /locus_tag="WJK66_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEZ7578140.1"
                     /translation="MAAFKPNPINYILGLDIGIASVGWAMVEIDEDENPICLIDLGVR
                     VFERAEVPKTGDSLAMARRLARSVRRLTRRRAHRLLRARRLLKREGVLQAADFDENGL
                     IKSLPNTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNEGETADKELGALLK
                     GVADNAHALQTGDFRTPAELALNKFEKESGHIRNQRGDYSHTFSRKDLQAELILLFEK
                     QKEFGNPHVSGGLKEGIETLLMTQRPALSGDAVQKMLGHCTFEPAEPKAAKNTYTAER
                     FIWLTKLNNLRILEQGSERPLTDTERATLMDEPYRKSKLTYAQARKLLGLEDTAFFKG
                     LRYGKDNAEASTLMEMKAYHAISRALEKEGLKDKKSPLNLSPELQDEIGTAFSLFKTD
                     EDITGRLKDRIQPEILEALLKHISFDKFVQISLKALRRIVPLMEQGKRYDEACAEIYG
                     DHYGKKNTEEKIYLPPIPADEIRNPVVLRALSQARKVINGVVRRYGSPARIHIETARE
                     VGKSFKDRKEIEKRQEENRKDREKAAAKFREYFPNFVGEPKSKDILKLRLYEQQHGKC
                     LYSGKEINLGRLNEKGYVEIDHALPFSRTWDDSFNNKVLVLGSENQNKGNQTPYEYFN
                     GKDNSREWQEFKARVETSRFPRSKKQRILLQKFDEDGFKERNLNDTRYVNRFLCQFVA
                     DRMRLTGKGKKRVFASNGQITNLLRGFWGLRKVRAENDRHHALDAVVVACSTVAMQQK
                     ITRFVRYKEMNAFDGKTIDKETGEVLHQKTHFPQPWEFFAQEVMIRVFGKPDGKPEFE
                     EADTPEKLRTLLAEKLSSRPEAVHEYVTPLFVSRAPNRKMSGQGHMETVKSAKRLDEG
                     VSVLRVPLTQLKLKDLEKMVNREREPKLYEALKARLEAHKDDPAKAFAEPFYKYDKAG
                     NRTQQVKAVRVEQVQKTGVWVRNHNGIADNATMVRVDVFEKGDKYYLVPIYSWQVAKG
                     ILPDRAVVQGKDEEDWQLIDDSFNFKFSLHPNDLVEVITKKARMFGYFASCHRGTGNI
                     NIRIHDLDHKIGKNGILEGIGVKTALSFQKYQIDELGKEIRPCRLKKRPPVR"
     gene            21569..22483
                     /gene="cas1"
                     /locus_tag="WJK66_000377"
     CDS             21569..22483
                     /gene="cas1"
                     /locus_tag="WJK66_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEZ7578141.1"
                     /translation="MTWRSLLIQNGGKLSLQRRQLLIQQNGESHTVPLEDIAVIIIEN
                     RETLITAPLLSALAEHGATLLTCDEQFLPCGQWLPYAQYHRQLKILKLQLNISEPLKK
                     QLWQHIVRQKILNQAFVADETGNDLAAKRLRTLASEVRSGDTGNREAQAAALYFQALF
                     GEKFTRNDNNAVNAALNYTYAVLRAAVARALTLYGWLPALGLFHRSELNPFNLADDFI
                     EPLRPLADLTVIHLYEQGRLKTELTLGIKQHLIKILYYQTSIERQHFSTLAAIDKMIS
                     SFQAGVTDKNAKQLKLPEILPLKEYQYE"
     gene            22476..22802
                     /gene="cas2"
                     /locus_tag="WJK66_000378"
     CDS             22476..22802
                     /gene="cas2"
                     /locus_tag="WJK66_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEZ7578142.1"
                     /translation="MSEAKFMRIIVFFDLPVITAAKRKAANQFRQFLLKDGYQMLQLS
                     VYSRIVKGRDSLQKHHNRLCANLPQEGSIRCLEITEKQYAAMKLLLGELKTQEKKVNS
                     DQLLLF"
     repeat_region   22866..23297
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=22866..22901
                     /rpt_unit_seq="attgtagcactgcgaaatgagaaagggagctacaac"
BASE COUNT         5765 a         6981 c         5797 g         4783 t
ORIGIN      
        1 aaattcttga ggcattgaaa ttccagattc ccgcctgcgc gggaatgacg gctcaaaagt
       61 tacggaacga aaaacaacca aaaccggaca agtcggattc ccgcctgcgc gggaatgacg
      121 gaatcttaag tttccgtctt tgttttctgt tttctgtttt cgagggaata acggggaaca
      181 agccgtattt cagacggcat tttcagttcg gggtataatc cgaatacttg cgaccatctg
      241 aatcattggg acaaaccatg tgtcaactgc tgggcatgaa ctgcaatacg ccgaccgata
      301 ttatgttttc ctttgaaggc ttccgccgca ggggcggcat taccgaccac catgccgacg
      361 gtttcggcat cggctttttc gaaggcaaag gcgtgcgcct gttccatgac gacaaaccca
      421 gtgccaattc gcccgtcgcc gacctcgtgc gcgcctacca gattaaatcg gaaaacgtcg
      481 tcgcccatat ccgcaaagca tcgcaaggac aaacctcgct ggcgaacacc catcccttta
      541 tgcgcgaaat gtggggtggc tactggctgt ttgcccacaa cgggcatttg attgattttt
      601 tccccgaaca gggcgaattt ttccaccccg tcggcacaac cgattccgaa cgcgcgttct
      661 gccacatcct caaccgcctg cgcacccgct ttgccgcccg tcccgacgac gacacgctgt
      721 ttgacgcgat tgcggggctg acgcacgaaa tccgcaagtt cgggctgttt aactttatgc
      781 tttcagacgg cattgccctg tttgcccacg ccagcacact gctgcactac atcgtccgcc
      841 aagccccgtt cggcaaggcg cgcctgctcg acgacgacgt gatggtcgat tttgccgaag
      901 taaccacgcc ctccgaccgc gtcgccgtta tcgccaccct gccgctgacc cgcgacgaat
      961 catggtccca acttgccgtg gacgaactgg tcatgttccg cgaaggcaac atcgtccgac
     1021 acgaccgtcc cgaaaacccc gtttatatga gtgtcgaaga aggtttggaa accgcgcgcg
     1081 ccgccggcgt cgccgtctga acttcagacg acataggagg acgaacccga tgaaatgccc
     1141 gttttgcgcc caccccgaca cccgcgttgc cgattcgcgc ctgatggacg aacgcaacgc
     1201 cgtccgccgc cgccgatact gccccaactg cggcaaacgc ttcggcacgc tcgaaaccgc
     1261 cgaactcaaa atgcccgccg tcatcggtcc ggacaaaaaa cgttcgccct ttaatgcaca
     1321 acgcctccgc aacgacctga ccgccgccgc ccgaaaatcc gccctgacac ccgaacagat
     1381 cgacgaaacc gtccgcctga cggaacacag gctctacact tcgggtcagc gcgacatccc
     1441 ctctgccgca cttgccgaca tggtgctaaa agaactgctc cgacaagata cggaagccgc
     1501 cgtccgtttc gccgccctgc acaaacgctt cgacaatccg gcagactttg cctcgtggct
     1561 ggcgcaagcc gtcaaaacag accgcaaagc ctgattcccc caacccatac tgatacggta
     1621 tccccatgtt ttccgaaaca gatatttcta tgatgcaaaa cgccctcaaa cttgccgctt
     1681 tggggcgttt ttccacttcg cccaatccgc gcgtcggctg cgttatcgca cacggcagcc
     1741 aaattgtcgg gcagggcttc cacgtcaaag cgggcgaacc gcacgccgaa gtccacgccc
     1801 tgcatcaggc gggggaaatg gcaaaaggcg cgaccgcctt tgttaccctc gaaccgtgca
     1861 gccattacgg gcgtaccccg ccctgtgccg aagctctgct ccgttccggc gtaacacgcg
     1921 tcgttgccgc catgcgcgac cccaacccgc tggttgccgg caaagggctt gccctgctcg
     1981 aatcggcagg catcaaaaca gaatgcggac tgcttgaaaa caaagcacgc gaactcaacc
     2041 ggggattttt atcgagcatc gaacgccgcc gcccctttgt ccgcctcaaa tgcgccgttt
     2101 cgctggacgg caaaaccgcc ctttcagacg gcagcagctt ttggattacc ggcgaagacg
     2161 cgcgtgccga cgtacaggtt ttgcgtgccg aaagctgcgc ggtgctgacc ggcatcggca
     2221 cggtgttggc ggacaatccc cggctcaacg tccgcgcttt tccaactttg cgccaacccg
     2281 cacgcatcgt gttagacagc cgcctgcgcc tgcccttgga cagccacctt ctgaccgacg
     2341 gacaatctcc aacctacatc gccacactcg aacgcaacga agacagactg cacccctatc
     2401 gggaacacgc acacatccgc atcctgatgc cgtctgaaac ggcaggcggc aaaatcgacc
     2461 tgaaccacct gatgcgcctc cttgccgacg aaggtttcgg agaaatcatg gtcgaagcag
     2521 gctccgaact cacatccgca tttctggcag aagatctggc ggacgaaatc gtgctgtacc
     2581 gttcgcccaa aatcctcggc agcggcaaag acctgctttc cctgcccgaa aaccgcgccg
     2641 ccctttccgc accgcccttg tggacacccg tttcaagcga aatcctcgga cacgacatca
     2701 aaaccgtgtt ccggaaaaac gataacgcct tttaaagggt ttgcgccgtt tcactatata
     2761 ataacgccga taaaaaacgg ccccgttcag accgccgccc gcccggaaaa gcgcaacccg
     2821 gactgccgca ccccgccggc agccgcaacg gactgaaagc cgtcaaattc cgatcaagaa
     2881 aggcttcaga cggcacaggc agcatcccgc cgccgcccgg acatcaaaaa tggacacaaa
     2941 agaaatcctc ggctacgcgg caggctcgat cggcagcgcg gttttagccg tcatcatcct
     3001 gccgctgctg tcgtggtatt tccccgccga cgacatcggg cgcatcgtgc tgatgcagac
     3061 ggcggcggga ctgacggtgt cggtgttgtg cctcgggctg gatcaggcat acgtccgcga
     3121 atactatgcc accgccgaca aagacacctt gttcaaaacc ctgttcctgc cgccgctgct
     3181 gtctgccgcc gcgatagccg ccctgctgct ttcccgcccg tccctgccgt ctgaaatcct
     3241 gttttcgctc gacgatgccg ccgccggcat cgggctggtg ctgtttgaac tgagcttcct
     3301 gcccatccgc tttctcttac tggttttgcg tatggaagga cgcgcccttg ccttttcgtc
     3361 cgcgcaactc gtgtccaagc tcgccatcct gctgctgctg ccgctgacgg tcgggctgct
     3421 gcactttccg gcgaacaccg ccgtcctgac cgccgtttac gcgctggcaa accttgccgc
     3481 cgccgccttt ttgctgtttc aaaaccgatg ccgtctgaag gccgtccggc gcgcaccgtt
     3541 ttcatccgcc gtcctgcatc gcggcctgcg ctacggcata ccgatcgcac taagcagcat
     3601 cgcctattgg gggctggcat ccgccgaccg tttgttcctg aaaaaatatg ccggcctaga
     3661 acagctcggc gtttattcga tgggtatttc gttcggcgga gcggcattat tgttccaaag
     3721 catcttttca acggtctgga caccgtatat tttccgcgca atcgaagcaa acgccccgcc
     3781 cgcccgcctc tcggcaacgg cagaatccgc cgccgccctg cttgcctccg ccctctgcct
     3841 gaccggcatt ttctcgcccc tcgcctccct cctgctgccg gaaaactacg ccgccgtccg
     3901 gtttatcgtc gtatcgtgta tgctgccgcc gctgttttgc acgctggtag aaatcagcgg
     3961 catcggtttg aacgtcgtcc gaaaaacacg cccgatcgcg ctcgccacct tgggcgcgct
     4021 ggcggcaaac ctgctgctgc tggggcttgc cgtaccgtcc ggcggcgcgc gcggcgcggc
     4081 ggttgcctgt gccgcctcat tttggctgtt ttttgttttc aagaccgaaa gctcctgccg
     4141 cctgtggcag ccgctcaaac gcctgccgct ttatatgcac acattgttct gcctggcctc
     4201 ctcggcggcc tacacctgct tcggcacgcc ggcaaactac cccctgtttg ccggcatatg
     4261 ggcggcatat ctggcaggct gcatcctgcg ccaccggaaa gatttgcaca aactgtttca
     4321 ttatttgaaa aaacaaggtt tcccattatg aaaatcgttt tgaccacatc tatggcaggc
     4381 ttgggcggca cggaaaccgc caccgtccgc ctcggcaggc tactcaggca gcacggacac
     4441 gacatcatgc ttgcctcttc ggacggaccg ttgctcaaag aggcgcaagc cttgggcata
     4501 cgttggcaac ccgtcgattt ctatcgcggc ggccttttcg gctacctcaa aggtatgatt
     4561 gcctacgccc gaatgctgcg gcgcgaacaa cccgacatca tcgactgcca aatggcgcgc
     4621 gtcgtacccg cctgcgccat tgccgccaaa atcgtttcgc cgaaaaccaa aatcatctac
     4681 cactcgcacg gtttggacgc ggcaacctac cccaaaatcg ccaaactctt cgacaagctg
     4741 ggcgtgtaca tcatcggtaa ctgcaaacac gagcgcgaaa aactcatccg ccacggtttc
     4801 cccgccggcc ggattgccta cgcctacaac gcactccccc cccccccgga atttcatttc
     4861 cggaaaaccg aaaaagagtg cgccgtactc ggcacgcttt cccgtttgga caccgtccgc
     4921 gccgtgcatc tgatgttgga tattttcaaa aaaatggttg accgcggtat gcccgtccgc
     4981 ctgaacgtgg cgggcatagg cgaagaaatg gacaacctca aagcccaagc caaacgtttg
     5041 ggtattgaag acaaagtcac cttcctcggc ggcatccgcg atttgaccgc ctacttcaaa
     5101 gacgtggata ttttggtgaa cacgccccac tgcatcggcg accacggcgc aggtgtgggc
     5161 aacaacatct tagaagccgg actttacgac acacccgtcg ttacctacga tatggcgggc
     5221 atttccgaaa tggtcgtcaa cggggaaacg ggttactgca tccccttcgg cgacgaagaa
     5281 gcctttatcg ccgccgtaga tacgctcgtc agacagccgc tgttgcgtga agcaatgggc
     5341 aaaaggctgc acgcacacgt cgccgcactc tgttccgacg acgaaatcta ccgtaccacc
     5401 atggcggcgt ataccatgta atgccgtctg aaagccgccg atgaacatca ccatagtcgc
     5461 cccttattgc tcgctgccgt ccgaaccgta tttcaaccgc ttttggtatc tggcagaacg
     5521 attgtcgcaa tcgcacgacg tgttgctgat taccagccgt ttccgccact acgacaaatc
     5581 cttcagacgg catgaagatg ctgccgccac ttcaaacggc aggctgcgcg tcaagctgct
     5641 ggacgaaccc ggctaccgaa aaaacgtctc cctcgcccgc gtcgccagcc accgcgtatt
     5701 cgtccgcaat ttggcacgct ggctgcacag cccgcaggcg gcggaacagg acatcgtcta
     5761 ttctgcctat ccgctgatgg cgaccaacct gctgttgggc aaacacaaag cgcgtttggg
     5821 ctacaaactg attgtcgatg tgcaggacgt atggccggag tctttctcct cggtcgtacc
     5881 attgttgaaa aaagtaccgc acaagctgct gccctttgct tcacgcgcca accgtgccta
     5941 ccgctgcgcc gacgcgctgg ttgccgtatc gcagacttat ctcgaccgcg ccaaagaagc
     6001 caatccgaac gtacccggcg aagccgtcta tatcggtacg gattttgccg ccatcgcccc
     6061 ccccccccgc gattccgctc caaaaccacc cgccttttct acttgggaac gctcagttac
     6121 agctacgacg tggaaaccgt gtgcaaaggt gttcggaaac ttttagacga cggcgaaaac
     6181 gtcgaattgc acattatggg cggaggaccc gatttggaga aactcaaaca atatgaaaat
     6241 cgcgccatca agttttacgg ttatttaccc tattctgaaa tgatgtcgat tgccaaaggc
     6301 tgcgacattg ccgtcaacgc catccatagc cacgccatgc agtcggttac caacaaactc
     6361 tccgactata tggctttgca aaaacccgtg ctgaacagcc agaccaatgc cgaagtcctc
     6421 gacctcatga atctgctgcc gcacgaaaac taccgttccg gcgatgtgga cagcttcgtt
     6481 caagccgcca aaaatatttt gaaacgcaaa gacgaccccg ttcagtccga cgaaatcgtc
     6541 cgccgcttca ggcgcgacgt tgcctatcaa aaaatcgcca acctgattga aagattagct
     6601 gatgaataaa tttttcaaac gcctgttcga catcatcgca tccgcatcgg ggctgattgt
     6661 cctctcaccc gttttcctga ttttggcgta cctcatccgc aaaaatctag gctcgcccgt
     6721 gttcttcatc caagagcggc cgggcaaagg cggcaaaccg tttaaaatga ttaaattccg
     6781 ctctatgcgc gatgcggtcg ataaatacgg caacccgctg ccggacagcg aacgcctgac
     6841 cgatttcggc aaaaaactgc gtgccgccag cctggacgaa ctgcccgaac tgtggaacgt
     6901 cctcaaaggc gacatgagcc tggtcggccc gcgcccgctg ctgatgcaat atctgccgct
     6961 gtacgacaac ttccaaaacc gccgccacga agtgaaaccc ggcattaccg gctgggcgca
     7021 ggtcaacggg cgcaatgcca cttcatggga cgaacgcttc gcatgcgaca tctggtatat
     7081 cgaccacttc agcctgtgcc tcgacatcaa aatcctactg ctgacggtta aaaaagtatt
     7141 aatcaaagaa gggatttccg cacagggcga agccaccatg ccccctttca caggaaaacg
     7201 caaacttgcc gtcgtcggtg cgggcggaca cggcaaagtc gttgccgagc ttgccgccgc
     7261 actcggcaca tacggcgaaa tcgtttttct ggacgaccgc gtccaaggca gcgtcaacgg
     7321 cttccccgtc atcggcacga cgctgctgct tgaaaacagt ttatcgcccg aacaattcga
     7381 catcgccgtc gccgtcggca acaaccacat ccgccgccaa atcgccgaaa aagccgccgc
     7441 gctcggcttc gccctgcccg tcctggttca tcccgacgcg accgtctcgc cttctgcaac
     7501 agtcggacaa ggcagcgtcg ttatggcgaa agccgtcgta caggctggca gcgtattgaa
     7561 agacggcgta attgtgaaca ctgccgccac cgtcgatcac gattgcctgc ttgatgcttt
     7621 cgtccacatc agcccgggcg cgcacctgtc gggcaacacg cgtatcggcg aagaaagctg
     7681 gataggcaca ggcgcgtgca gccgccagca gatccgtatc ggcagccgcg caaccattgg
     7741 agcgggcgca gtcgtcgtgc gcgacgtttc agacggcatg accgtcgcgg gcaacccgac
     7801 aaaaccattg gcaggcaaaa ataccgagac cctgcggtcg taaacgatta aaataaacag
     7861 cctaagcaac agacggcaga aatgaggaaa tgccgtctga aaaccgacgg accaaacacc
     7921 atgctgaaca ctttcctttc cccgtggccc tgcttcaccc aagaagaagc cgatgccgtt
     7981 tccaaagtcc tgctgtccaa caaagtcaac tactggacgg gcagcgaatg ccgcgaattt
     8041 gaaaaagaat ttgctgcttt cgccggcacg cggtacgccg tcgccctttc caacggcacg
     8101 ctggcactcg atgccgcgct caaagcaatg ggcataggcg cgggcgacga tgtgattgtt
     8161 acttcgcgca ccttcctcgc ctccgcctcc tgcatcgtga atgcgggcgc aaatcccgtg
     8221 tttgctgatg tggatttgaa cagccaaaac atcagcgcgg aaaccgtcaa ggccgtgctg
     8281 accccgaata ccaaagcgat tatcgtggtt caccttgccg gtatgcctgc cgaaatggac
     8341 ggcattatgg ctttggcaaa agaacatgat ttgtgggtga ttgaagactg cgcccaagcg
     8401 cacggcgcaa aatacaaagg caaatccgtc ggctctatcg gacacgtcgg cgcgtggtcg
     8461 ttctgccaag acaaaatcat gaccacgggc ggcgaaggcg gcatggttac caccaacgac
     8521 aaaaccctgt ggagcaaaat gtggtcgtac aaagaccacg gcaaaagcta tgacgccgtg
     8581 taccaccgcg aacacgcccc tggcttccgc tggctgcacg aaagcttcgg cacaaactgg
     8641 cgtatgatgg aaatgcaggc ggcaatcggg cgcattcagc tcaaacgcct gcccgaatgg
     8701 actgcctgcc gtcaagcgca cgccgccaag ctggcggaaa gcctaggcaa attcaaaagt
     8761 atccgcttgg ttgaagtcgc cgactacatc ggacacgcgc aatataagtt ctacgccttc
     8821 gtcaaacccg aacacctcaa agacggctgg acgcgcgacc gcatcgtcaa cgaactgaac
     8881 gcgcgcaaag tcccctgcta tcaaggtagc tgctccgaag tgtatttgga gaaatccttc
     8941 gacaacacgc cttggcgtcc gaaagagcgg ctgaaaaatg ctgtcgagtt gggcgacacc
     9001 agcctgatgt tcttagtaca tccgacgctg accgacggcg aaatcgcgtt ctgcaaagaa
     9061 cacatcgaag ccgtattgac cgaagccaca cgataaccct tcagacggca tatgccgcct
     9121 gaaagcacat accgccccac gatatgactc tggaaaccct gcttgcccta ccgcgcaaca
     9181 tcaagaaaat ctgtttcctc atacacgatt ttctgatgat tttcattgcc ttttggttta
     9241 cccaaagcct gaaggccgac tactcgaacg aatggttcga ccctgccaac tggcagtctt
     9301 tcctattgac cgcgccgttg accatcggcc tgttcgtcag aatggggctc taccgtgccg
     9361 ttacacgctt catcagcttc cgcatcctct ccaccgcgct ggcaggcagc ctcgcctccg
     9421 ccgtattgtt cttcctcaac acattgattt ttgaagaaag gctgcgcctc gccctgccga
     9481 ttgtctattt cttactgctg tttgtttccg tgaccggctc gcgtatggtt ttgcgcggac
     9541 tgttgtccga acaccccaaa aaacagatga tccctgtcat catttacggc gcgggacggt
     9601 cgggcagaca actgcttgag gccgtcaaac aaatgcgcga atattccgcc gccgcctttg
     9661 tagacgacga ccccaaactg tggcacaccg tcatctacga ccttgccgtt taccagcccg
     9721 atgccatcgc cttcctcatc gaacgctacg gcgtggaaaa aatcctgctc gccatccccg
     9781 gcgcgaccca ggaacaacgc cgccgaatca tcaacaaact ggaagcctat ccgtgcgaag
     9841 tgttgaccat tcccggaatg aaagacctga tggacggaaa aatcagcatc ggcacgctca
     9901 aaaaaatctc tgtgtccgac ctgctcgggc gtgattccgt cgcgcccgac gaccgcctga
     9961 tgagtgccga catcgaaggc aaaaccgtca tggtaaccgg cgcgggcggc tccatcggtt
    10021 cggaactctg ccgccagatt atccgccgcc gccccgaaaa gctgctgctg ttcgagttat
    10081 ccgaattcgc cctgtacgcc atcgaaaaag aattgcgcga aacctgcatc caaaaacgcc
    10141 tcgacaccga aatcctgccc tttctcggtt cggtgcaaaa ccgcacgctg ctcgaacacg
    10201 tcatgaccgc cttttccgtt gcgaccgtct atcacgccgc tgcctacaaa cacgtcccca
    10261 tggtcgagtt caacaccgtc gaaggcatac gcaacaacat cttcggcaca ctcgagtgcg
    10321 cgcttgccgc cacgacatcg ggcgtaagaa ctttcgtcct catctccacc gacaaagccg
    10381 tccgccccac caacaccatg ggtgccagca aacgcatggc ggaactctgc cttcaggcac
    10441 tcgccgccga acccggacaa aaaacccgct tcagcatggt acgtttcggc aatgttttag
    10501 gttcgtccgg ctccgttgtc ccgctgtttg aaaaacagat tgcagaaggc ggcccgctta
    10561 ccctgaccca ccccgaaatc acacgttatt tcatgaccat acccgaagcc gcccaactcg
    10621 tcatacaggc aggcgcgatg ggtacgggcg gcgacgtatt cgtcctcgac atgggtgaat
    10681 ccgtcaaaat catcgacctt gcccgccaaa tgattaccct aagcggcctc aaacccaaca
    10741 cacccgaaca acccgacggc gacatcgaaa tcctcattac cggactgcgt cccggcgaaa
    10801 aactctacga agagctgctc atcggcgaca acgtccgcaa aaccgcccat ccgcgcatta
    10861 tgacggctga cgaggcgatg ctgccgtggc acgagctctc tgtcctgctc gaccgcatcc
    10921 gttcggcctg cgaccgttac gaccagcagg caatccgcac cctgctcatc aacgccccga
    10981 ccggctttgc cccgagcgac ggcatctgcg acctgctttg ggtacgagaa acacacagaa
    11041 aaaatgccgt ctgaaccttc agacggcata acgtacaaac caacctacct tacacacacg
    11101 gagtttgaca tgcagttctc agcattcggc gaaaaattca cgcaacacag cggcatcctc
    11161 caactgatgg acgacctcgg cgacgcgctc aaaagcgaca agcccgtcaa catgctcggc
    11221 ggcggcaatc cggcgcgcat tccggaaatc gatcgggcgt tcgccgacat attctccaaa
    11281 ctggcggcgg aacacgctgt cgagaacatc ggcaactact ccaatcccca aggcgatgcc
    11341 gcgctgattg acgcgctgac cgccttcctc aaccgcgaat acggctggaa cctgaccgcc
    11401 gacaatatcg cgctgaccaa cggttcgcaa aacgcgtttt tctatttatt caacctcttc
    11461 ggcggcaaat tcaacctttc agacggcaca tccgcagaaa aagccatttt gttgccgctc
    11521 gcgcccgaat acatcggcta tgccgacgtg catgtcgaag ggcagcactt cgtttccgtc
    11581 aagcccaaaa tcgaaaacgt cgaacacgaa ggcgaagcag gcttcttcaa ataccgcgtg
    11641 gactttgacg cactggaaaa cctgcccgaa ctcaaagcgg gcaaaatcgg cgcgatttgc
    11701 tgttcgcgcc cgaccaaccc gaccggcaac gtgctgaccg acagcgaaat ggcgcggctg
    11761 gacgctttgg cgcaagaaca cggcattccg ctgattatcg acaacgccta cggaatgccg
    11821 ttccccaaca tcatttacag cgacgtaacg ctgaattggc atgaaaacat catcctctgc
    11881 ttcagccttt ccaaagtcgg tctgccgggc gttcgcaccg gcatcatcgt cgccgcgccc
    11941 gaagtcgtca aagccgtcag cagcctgaac gcgattgtga accttgcccc cacgcgcttc
    12001 ggcgcggcca tcgccgcccc gctgctggaa agcggcgaga tgaaacggct tgccgaccaa
    12061 gtcatccggc cgttttaccg caatcaggcg caaaccgccg tctcgctgct caagcgcgag
    12121 ctgggcgcgt acccgatgaa aatccacaaa cccgaaggcg cgattttcct gtggctctgg
    12181 tttgaaaacc tgcccgtttc ttcgcaaacc ctgtacgaaa tgctcaaagc cgaaggtacg
    12241 ctgattattc cgggcgagca tttcttcgtc ggcatcgaca cgcgggatta cccgcacgcg
    12301 ggcgagtgca tccgcatgag catcgcgcag gacgctcaaa cgctggaaaa aggcatcgcc
    12361 gccatcggca aaaccgtccg aaaactgtac gacaacgttt aaaccgcaaa aaatgccgtc
    12421 tgaaaggttt tcagacggca tttttatctg cattcaatat cgggaaaaat gcgcccacac
    12481 cggcctgcag ttttccggca gttcgggaca cgcgccgaga atgccgccgc taaagtcgtt
    12541 taaacggtgg aagtcgctgc ccgcgctggc gagcataccg aagcgttctg ccaaaagcgc
    12601 gtagttgagg cggtcgtttt tgcagcagtt gccgctgtgg acttcgatgc ctgcgccgcc
    12661 gaggttttta aattcttcaa acagattgcg cttggcggtg gcggacaaat cataacgcat
    12721 cggatgggcg atgaccgcca acccgcccgc gccgttgacg gcggagacgc agtcttccag
    12781 cgtcgcccat tcgtggcgga cggcgcagga tttgccgtcg cccaagtatt tggtgaacgc
    12841 ctgctgcttg tttttgacgt gtcccacccg aatgaggaac tcggcgacgt gtgtgcggct
    12901 gaccatttct ttgtttgccg ccagcgcgag cgcgccgtcg tatgcgcccg aaatgccgcg
    12961 tttttccagc ttggcggcga tggcttcgag gcgtttcaga cggccttgcc gcacttgcgc
    13021 caacaggttt tgcaggtttt cgtcttgttc gtcaaagtct aagccgacaa cgtgtatggt
    13081 acgcccgcgc cacgttacgg agatttccac gccgttaatc aggcgcaaac cgagcctgtc
    13141 ggcttcggca cgcgcttcgg cgatgccgcc ggtgtggtcg tggtcggtca acgccagcag
    13201 cgtgcagccg ttttgatgcg cgaggcgcac aacttcggcg ggggagagca taccgtcgga
    13261 aacggtggaa tggcagtgca ggtctatcat aaggcgttat gtgcgtggta aatggtgggc
    13321 agggtgtttc aatattgtcg ggcggtgtgg cggcggtgat ttcaaaaaac aagtgtatgg
    13381 caaatgcaaa ggaaaagccg ctatgccgtc attcccgcgc aggcgggaat ccagactttg
    13441 atttattgga aatgtttaag gttaaccgct atttcaaact tccggattcc tgctttcgag
    13501 ggaatggcga tatgaccgtt ttcaatttta atctactata aaagactgtc tgaaaacgtg
    13561 gttttatcaa gccgaactgg tttcagacgg cattttcgtc ttccgcccaa cccgcttctt
    13621 tcaacttccg ccgttgcgcg tcgtagcgtg ccttatccgc ccagtaaatc gtctgaatgc
    13681 acgcctcgcc gcagtcgctg ccgcagcatt cccacaactc agggcggacg ggttcttcta
    13741 aaagcggctc gcccaaaagg gcttcggctt tatacttcag ggtcgtatcc atcggcgatt
    13801 tccaagcgcg cgccgtcaaa ctcgatgact tcgccgccgc gtattttggc ggttttacgg
    13861 gtttcggttt cgccgttgcg caacaccagc ccttcggcga taaatgcttt tgcctgtccg
    13921 ccgctttcgg caagtccggc caatttcaag aggtcgcaca gggcgatgta ttcgttgtct
    13981 tcgagataga cggtggcttc cataatgttc ccttgcagaa agaggccgtt attgtagcac
    14041 ctgccgccgc cgtacccaaa attaccgcaa aaccggcagc acatcccaca ccagctgttc
    14101 cgtaaaagta aaaatgccgt ctgaaacccc atatgccgcc atccgatcaa agaaatcctg
    14161 tccaacggca agctgcaaat cctgttcccc gacgaatccg cattgacgct gatgcacatc
    14221 ctcaaacgcg aactgcccga tacaccggca atcggcatca aaacgaaatc aaaaacctgt
    14281 ttgaacgtaa aatcgattta actgcttgcc atccgtccga ataatgtata tagttcttta
    14341 taactagttt gatagtcctt tatatctatc aatactcctt gggaagcctc caccacacgg
    14401 taggaggcat ttttttgccg tcgtaaaagc tcaaaaacat ttgcaggtca tgccgtctga
    14461 atccgaaaca gcattaccta caccgccatc taaagacaac cctgctacaa tacgcctttt
    14521 attgttcacg ccgattttgc catgaccgag ccgacctaca tccccctgcg cctgcatacc
    14581 gaattctcga ttaccgacgg tatggtgcgg attaaaaaac tgattgccaa agcgcaggaa
    14641 tacggtttgc ctgctttggg catcagcgat ttgatgaacg aattcggttt ggtgaaattc
    14701 tataaaacct gccgcggcgc agggattaaa cccatcggcg cggcggacgt gtggataggc
    14761 aatccgaatg cgcccgacaa gccgttccgc gccatgctga ttatccgcaa cgatgcgggc
    14821 tatctgcgct tgagcgagct tctgacggcg gcttatgtcg gcaaagaccg caatatccat
    14881 catgcggaac tcaatcccga atggctggaa aacggcgaca acagcggctt gatttgttta
    14941 agcggcgcgc attacggcga agtgggcgtg aatctcttga acggcaatga agacgcggcg
    15001 cgtgcggcgg cgttgaaata tgcggcatgg tttcccgatg cgttttattt agagctgcaa
    15061 cgcctgcccg aacgccccga atgggaggct tgcgtttccg gcagcgtgaa gctggcggag
    15121 gaactgggtt tgccggtggt ggcgacccac ccgacgcagt ttatgagccg cgacgatttc
    15181 aacgcgcacg aggcgcgggt gtgtatcgca ggcggctggg tattgacgga caaaaaacgt
    15241 ccgcgcgatt tcacgccggg ccagtttttc attccgccgg aaaccatggc cgaacgtttc
    15301 gccgatttgc ctgaagcctt ggaaaacacg gtagaaattg ccaaacgctg caacctgcac
    15361 atcacattgg gcaaaaactt cctgcccctt ttccccacgc ccgacggttt atcactcgat
    15421 gactatctca tcaaactctc caacgagggt ttgcaggaac gcatggttca gctttatccc
    15481 gacgaagcgg aacgcgcaga aaaaatgccg gaatatcagg aacgtttgga ttttgagctg
    15541 aacatcatca tccagatgaa atttcccggc tatttcctta tcgtacaaga ctttatcaac
    15601 tgggcaaaaa cgcacggctg tccggtgggg ccggggcgcg gttcgggcgc aggctcgctg
    15661 gtggcgtatt cattgaagat taccgacctt gatccgctca aatacgcgct gctgtttgaa
    15721 cgtttcttaa accccgaacg cgtctccatg cccgacttcg acgttgactt ctgtcaggca
    15781 aaccgcggcc gcgtgattga gtatgtgcgc gaaaaatacg gcgcggaggc ggtcagccag
    15841 attgttacct tcggcacgat gtcatctaaa gcggtcatcc gcgacgtggg gcgcgtgtta
    15901 gagctgccgt ttatgctgtg cgacaaactg tccaagctga ttccgttgga agccaacaaa
    15961 cctctgggtt tggacgacgc gatgaaggcg gagccgcaga ttcaggaatt aatcgaagcg
    16021 gaagaagcgg acgaactcat tacgctggcg aaaaagctgg aagatttgac gcgcggtctg
    16081 ggtatgcacg cgggcggcgt gttgattgcg ccgggcaaga tttccgatta cagccccgtg
    16141 tatcaggcgg atgaatccgc ctctcccgta tccatgtacg acaagggcga cgtggaagac
    16201 gtgggtttgg tgaaattcga ctttttaggt ctgcgtaacc tgaccattat cgaaatggcg
    16261 cagaacaaca tcaaaaacac caccggcgat atcgtcgatg tcggcaaaat tccgctcgac
    16321 gaccaagccg cctaccaaat cttccgagat gcgaacacca ccgccgtgtt ccagttcgag
    16381 tcgaccggca tgaaaaaaat gctgaaaacg gcgcatacga ccaagtttga agaactgatt
    16441 gccttcgtat cgctctaccg ccccggcccg atggacaaca tccccgactt cgtcgcgcgc
    16501 atgaaaggcc aggaattcca atacatccat ccgctgctcg aaggcatcct cgcgccgacc
    16561 tacggcatta tggtgtatca ggaacaagtg atgcaggcgg cgcagattat cggcggctac
    16621 tcgctcggcg gcgcggactt gctgcgccgc gcgatgggta agaaaaagcc cgaagaaatg
    16681 gtgaaacacc gcgaaatctt cgccgaaggc gcggcgaaac aaggcatttc gcgcgaaaaa
    16741 tccgacgaaa tcttcaacta catggaaaaa ttcgccggct acggtttcaa caaatcccac
    16801 gccgccgcct acgccctgat ttcctaccag accgcatggc tcaaggcgca ctaccccgcc
    16861 gaatttatgg cggcgacgat gtcgtccgaa ttggacaaca ccgaccagct caagcatttc
    16921 tacgacgact gccgcgccaa cggcatcgag ttcctgccgc ccgacatcaa cgaatccgac
    16981 taccgcttca cgccgtatcc gaacatgaaa atccgctacg cactcggcgc gattaaaggc
    17041 acgggcgaag ccgccgtcga atccatcatc gccgcgcggc aaagcggcgg caagtttacc
    17101 ggcctcttgg acttctgcga gcgcgtcggc aaagaacata tgaaccgccg caccctcgaa
    17161 gccctgatac gcggcggcgc gttcgacagc atcgaaccca accgcgccat gctcttggca
    17221 aacatcgacc tcgccatgaa caacgccgac caaaaagccg ccaacgccaa tcagggcggg
    17281 cttttcgaca tgatggaaga cgccatcgaa ccggtgcggc tcattgacgc gccgatgtgg
    17341 agcgaatcgg aaaaactcgc cgaagaaaaa accgtcatcg gcttttacct gtccggccac
    17401 ccgttcggcc cgtatgccca agaagtccgc caaatcgccc cgcagaaact gtcgaaactc
    17461 aaaccgcaag acagcgtgcg cctcgccgga ttcgttaccg ccgtgcgtac gatgatgggc
    17521 aagcgcggca aaatcgcctt cgtcagcctt gaagatttga gcgggcaggt tgaaatcatg
    17581 gtcggcggtc agacgttgga aaactgcgcc gactgcctca aagccgacca agtgctgatt
    17641 atcgaatcca aagtcagccg cgacgactac ggcggcggcg acgggctgcg tattctggca
    17701 aaccaagtca tgaccctgca aacggcgcgc gaacgctacg cccgcagcct cagcctcgcc
    17761 ctcgccccgc atcacgacat cggcgaactg gtacaactgc ttgccgccca ccaactgccc
    17821 gacacgccgc gcatcccgct gcaactgtcg tatgccaacg aaaaagcgtc gggcaggctt
    17881 caagtgccgc cgaaatggac ggttacaccg agctccgcat tgttcggcga actggaaaca
    17941 ttgctcggca gccggtcggt gcgcgtcaac tggtaaccca aaatataaat gccgtctgaa
    18001 gcccaaaatc ggtttcagac ggcatttttt aaccgaaata aacgatgccc cttaaagcag
    18061 aagctttaag gggcagagcg ttgcggcaca tcttttcaga cggccttatt gtagcaacgg
    18121 ttctcatttc gcagtgcgac aatatgtttg ggattctagc cgttgtgaga tgataagtca
    18181 ataatctatt tgtatataaa taagacaata agatatatta tcattatttg caacgttgtt
    18241 tacggagtaa taaatggctg ccttcaaacc taatccaatc aactacatcc tcggcctcga
    18301 tatcggcatc gcatccgtcg gctgggcgat ggtagaaatt gacgaagatg aaaacccaat
    18361 ctgcctgatt gatttgggcg tgcgcgtatt tgagcgtgcc gaagtaccga aaacaggcga
    18421 ctcccttgcc atggcaaggc gtttggcgcg cagtgtccgc cgcctgaccc gccgtcgcgc
    18481 ccatcgcctg cttcgggccc gccgcctatt gaaacgcgaa ggcgtattac aagccgctga
    18541 ttttgacgaa aacggcttga tcaaatcctt accgaataca ccatggcaac ttcgcgcagc
    18601 cgcattagac cgcaaactga cgcctttaga gtggtcggca gtcttgttgc atttaatcaa
    18661 acaccgcggt tatttgtcgc aaagaaaaaa cgagggcgaa actgccgata aagagcttgg
    18721 cgctttgctc aaaggcgtag ccgacaatgc ccatgcctta cagacaggcg atttccgcac
    18781 accggccgaa ttggctttaa ataaatttga gaaagaaagc ggccatatcc gcaatcagcg
    18841 cggcgattat tcgcatacgt tcagccgcaa agatttacag gcggagctga ttttgctgtt
    18901 tgaaaaacaa aaagaatttg gcaatccgca tgtttcaggc ggccttaaag aaggtattga
    18961 aaccctactg atgacgcaac gccctgccct gtccggcgat gccgttcaaa aaatgttggg
    19021 gcattgcacc ttcgaaccgg cagagccgaa agccgctaaa aacacctaca cagccgaacg
    19081 tttcatctgg ctgaccaagc tgaacaacct gcgtatttta gagcaaggca gcgagcggcc
    19141 attgaccgat accgaacgcg ccacgcttat ggacgagcca tacagaaaat ccaaactgac
    19201 ttacgcacaa gcccgtaagc tgctgggttt agaagatacc gcctttttca aaggcttgcg
    19261 ctatggtaaa gacaatgccg aagcctcaac attgatggaa atgaaggcct accatgccat
    19321 cagccgtgca ctggaaaaag aaggattgaa agacaaaaaa tccccattaa acctttctcc
    19381 cgaattacaa gacgaaatcg gcacggcatt ctccctgttc aaaaccgatg aagacattac
    19441 aggccgtctg aaagaccgta tacagcccga aatcttagaa gcgctgttga aacacatcag
    19501 cttcgataag ttcgtccaaa tttccttgaa agcattgcgc cgaattgtgc ctctaatgga
    19561 acaaggcaaa cgttacgatg aagcctgcgc cgaaatctac ggagaccatt acggcaagaa
    19621 gaatacggaa gaaaagattt atctgccgcc gattcccgcc gacgaaatcc gcaaccccgt
    19681 cgtcttgcgc gccttatctc aagcacgtaa ggtcattaac ggcgtggtac gccgttacgg
    19741 ctccccagct cgtatccata ttgaaactgc aagggaagta ggtaaatcgt ttaaagaccg
    19801 caaagaaatt gagaaacgcc aagaagaaaa ccgcaaagac agggaaaaag ccgccgccaa
    19861 attccgagag tatttcccca attttgtcgg agaacccaaa tccaaagata ttctgaaact
    19921 gcgtctgtac gagcaacaac acggcaaatg cctgtattcg ggcaaagaaa tcaacttagg
    19981 ccgtctgaac gaaaaaggct atgtcgaaat cgaccatgcc ctgccgttct cgcgcacatg
    20041 ggacgacagt ttcaacaata aagtactggt attgggcagc gaaaaccaaa acaaaggcaa
    20101 tcaaacccct tacgaatact tcaacggcaa agacaacagc cgcgaatggc aggaatttaa
    20161 agcgcgtgtc gaaaccagcc gtttcccgcg cagtaaaaaa caacggattc tgctgcaaaa
    20221 attcgatgaa gacggcttta aagaacgcaa tctgaacgac acgcgctacg tcaaccgttt
    20281 cctgtgtcaa tttgttgccg accgtatgcg gctgacaggt aaaggcaaga aacgtgtctt
    20341 tgcatccaac ggacaaatta ccaatctgtt gcgcggcttt tggggattgc gcaaagtgcg
    20401 tgcggaaaac gaccgccatc acgccttgga cgccgtcgtc gttgcctgct cgaccgttgc
    20461 catgcagcag aaaattaccc gttttgtacg ctataaagag atgaacgcgt ttgacggtaa
    20521 aaccatagac aaagaaacag gagaagtgct gcatcaaaaa acacacttcc cacaaccttg
    20581 ggaatttttc gcacaagaag tcatgattcg cgtcttcggc aaaccggacg gcaaacccga
    20641 attcgaagaa gccgataccc cagaaaaact gcgcacgttg cttgccgaaa aattatcatc
    20701 tcgccccgaa gccgtacacg aatacgttac gccactgttt gtttcacgcg cgcccaatcg
    20761 gaagatgagc gggcaagggc atatggagac cgtcaaatcc gccaaacgac tggacgaagg
    20821 cgtcagcgtg ttgcgcgtac cgttaacaca gttaaaactg aaagacttgg aaaaaatggt
    20881 caatcgggag cgcgaaccta agctatacga agcactgaaa gcacggctgg aagcacataa
    20941 agacgatcct gccaaagcct ttgccgagcc gttttacaaa tacgataaag caggcaaccg
    21001 cacccaacag gtaaaagccg tacgcgtaga gcaagtacag aaaaccggcg tatgggtgcg
    21061 caaccataac ggtattgccg acaacgcaac catggtgcgc gtagatgtgt ttgagaaagg
    21121 cgacaagtat tatctggtac cgatttacag ttggcaggta gcgaaaggga ttttgccgga
    21181 tagggctgtt gtacaaggaa aagatgaaga agattggcaa cttattgatg atagtttcaa
    21241 ctttaaattc tcattacacc ctaatgattt agtcgaggtt ataacaaaaa aagctagaat
    21301 gtttggttac tttgccagct gccatcgagg caccggtaat atcaatatac gcattcatga
    21361 tcttgatcat aaaattggca aaaatggaat actggaaggt atcggcgtca aaaccgccct
    21421 ttcattccaa aaataccaaa ttgacgaact gggcaaagaa atcagaccat gccgtctgaa
    21481 aaaacgcccg cctgtccgtt aaattaatct atccctgttt caggcggcct gaagcaggga
    21541 ttttttatat aaaaataagg ggaaaaacat gacttggcgc agcctgctta ttcaaaacgg
    21601 cggcaaacta tccttgcaac gccggcaact gctgattcaa caaaacggcg agtcccacac
    21661 cgtaccatta gaggacatcg ccgttatcat catcgaaaat cgcgaaaccc tgattaccgc
    21721 cccgctccta tcagccttag ccgagcatgg tgcaaccctg ctgacctgcg acgagcagtt
    21781 cctgccctgc ggacaatggc tgccttatgc ccaatatcac cgccaactta aaatcctcaa
    21841 actgcaactg aacataagcg aacccttaaa aaagcaactc tggcaacata tcgtccgcca
    21901 aaaaatcctg aaccaagcat ttgtcgcaga cgaaacaggc aatgatcttg cagccaaacg
    21961 gctgcgcact ttggcatctg aagtccgttc aggcgataca ggaaaccgtg aagcccaagc
    22021 tgccgccctc tactttcaag ccctctttgg cgaaaaattt acccgtaatg acaacaacgc
    22081 cgtcaatgcc gcattgaact acacctacgc cgtactccgt gccgcagttg cacgcgccct
    22141 gaccttatac ggttggttac ctgcattagg cttgtttcat agaagtgaac tcaatccatt
    22201 taatctcgcc gatgacttta tcgaaccctt acgcccattg gccgacctga ccgtgataca
    22261 tttgtacgaa caaggccgtc tgaaaactga actgacgctc ggcatcaaac aacacctcat
    22321 caaaatcctc tactatcaaa ccagcatcga acggcaacat ttcagcacct tggctgccat
    22381 cgacaaaatg atttcgtcat ttcaagccgg cgtaaccgat aaaaacgcca aacaactgaa
    22441 actgcctgaa atcctaccat tgaaagaata ccaatatgag tgaggccaaa tttatgagga
    22501 ttatcgtctt cttcgaccta ccggttatca cggcggcaaa gcgcaaagcc gccaatcaat
    22561 tccgccagtt tttattaaaa gacggatacc aaatgctgca actttccgta tacagccgta
    22621 tcgtcaaagg ccgcgattcg ttgcaaaaac accacaacag actgtgtgca aaccttccgc
    22681 aagaaggctc aatccgctgt ttggagataa cagaaaagca atatgccgcc atgaaactgc
    22741 tgttgggcga gctgaaaacc caagaaaaaa aggtcaattc agaccaatta ttgttatttt
    22801 aagcccattt tttcataaca aataaaacgg gaaaccctta tgaaataagg atttcccgtc
    22861 gaagtattgt agcactgcga aatgagaaag ggagctacaa caccaacagg atgccgatga
    22921 tttgggtatg aattgtagca ctgcgaaatg agaaagggag ctacaacgtt ttgtggcaat
    22981 ggtttccgcc gtgggtaatt gtagcactgc gaaatgagaa agggagctac aacgatggta
    23041 attatcgttg ccatcatgcg caaattgtag cactgcgaaa tgagaaaggg agctacaaca
    23101 tgctcgagtt tccatcatcg acgaatacta ttgtagcact gcgaaatgag aaagggagct
    23161 acaacaccac cgcgtttgct atctgaaacg aatttattgt agcactgcga aatgagaaag
    23221 ggagctacaa caagcacctt gaatcaatac aaaagcgaaa tattgtagca ctgcgaaatg
    23281 agaaagggag ctacaacctg taccagttgc ccaccgtcat atccgg
//
LOCUS       DAWKOG010000012        22465 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.012, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000012 DAWKOG010000000
VERSION     DAWKOG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22465
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.012"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            56..619
                     /locus_tag="WJK66_000379"
     CDS             56..619
                     /locus_tag="WJK66_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ7578143.1"
                     /translation="MRHLENNGYANVAGLERILAVKTDNYKEKENLLHEIFSKSRIGD
                     TELFAVDENLVKRLFLSLRGEIVFPKNETAESEFEKSVHERRQEGNAGSGRKQLLDLV
                     RRGHREYPYALPRLLAGAASYKPKKSKIRLFKEAYFGKSGTRLTDEIADGIHIYTCFS
                     RADLEKAYSEYLELFKSESDAEGRKPQ"
     gene            656..1417
                     /locus_tag="WJK66_000380"
     CDS             656..1417
                     /locus_tag="WJK66_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HEZ7578144.1"
                     /translation="MMGDSVIYYVEQADKPVNRAGERARKTFKYFWRELFWERRRIIP
                     ALDFAMVKAPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGELTNVEQGESVCV
                     PVDDISDWMFVCNGIPYGGFTIQAMRGQMTEEERTEHDAAWGIDFGDPGQVLLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLAGWQNVADLLEPRH"
     gene            1472..1813
                     /gene="fdx"
                     /locus_tag="WJK66_000381"
     CDS             1472..1813
                     /gene="fdx"
                     /locus_tag="WJK66_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEZ7578145.1"
                     /translation="MPKITVLPHTTLCPEGAVIEDAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(2100..2687)
                     /locus_tag="WJK66_000382"
     CDS             complement(2100..2687)
                     /locus_tag="WJK66_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEZ7578146.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHYRSRRGLIALDKAAAGCDAAMLRAQRWRIYTQIAERLAGSGGGFT
                     VTAESVSAACPELEGRYLELVRRTATSFGFVQVQEYPAGGQEETQAVPDAASDHSVRK
                     PIKINIEAKVERYPAYRPTAALKVTRRRFFRLQNR"
     gene            2900..3097
                     /gene="iscX"
                     /locus_tag="WJK66_000383"
     CDS             2900..3097
                     /gene="iscX"
                     /locus_tag="WJK66_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEZ7578147.1"
                     /translation="MKWTDTRRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(3165..3566)
                     /locus_tag="WJK66_000384"
     CDS             complement(3165..3566)
                     /locus_tag="WJK66_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ7578148.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTATLREERKQL
                     DQFSRITSAYPDGRPTKRDRRQLDRLKKGDW"
     gene            3777..4736
                     /locus_tag="WJK66_000385"
     CDS             3777..4736
                     /locus_tag="WJK66_000385"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEZ7578149.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQVSQVSRHPQRPYTLDYIDALFTDFEELHGDRHFADDHAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLHVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            4804..6114
                     /gene="tilS"
                     /locus_tag="WJK66_000386"
     CDS             4804..6114
                     /gene="tilS"
                     /locus_tag="WJK66_000386"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEZ7578150.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKVCVEKNGLGIEAAAR
                     QKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPTVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNSDTAYLRNRFRHRILPELSAQIPHFGRHVLN
                     NVRALQEDLALLDEIIGQDCRWVCEEGSFDTVRWQAFSPRRKTHILRYFLKENGIPMP
                     NQNALADIARVLTEAKTGRWNLQGFELHHYAGRLFVFRLEKTDKLRFLKDRQISGNLR
                     EILTGQGFVLKRHPFGLPEHLLEQDGILRTVAVSDTLAVGGIHKDVKKILQGKRVLPV
                     LRPIWPLVADSGNRPLALANCCADFQISVSDGILPVHPDFPILF"
     gene            6188..6973
                     /locus_tag="WJK66_000387"
     CDS             6188..6973
                     /locus_tag="WJK66_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ7578151.1"
                     /translation="MRHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGGMPVGVYIPEGKMPSEEIRRLRAVVPEGRIFSVSDGILKKISSLSCADDILALIDI
                     PLGGTLPDKGDCVVLDGVQDPGNVGTVLRSAAAAGVGTVVLGRGCADAWSPKVLRAGM
                     GAHFLSGIYPQADLEIWLARYKGRVFATALREEKQAVLYGEDLCGPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRSAY"
     gene            6983..7180
                     /locus_tag="WJK66_000388"
     CDS             6983..7180
                     /locus_tag="WJK66_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578152.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILLSNTVAAAVLAWVWWKWF"
     gene            7442..7795
                     /locus_tag="WJK66_000389"
     CDS             7442..7795
                     /locus_tag="WJK66_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578153.1"
                     /translation="MKNCEFFYDPTRAIYDSGADYLTREKHRLVVIANSAWGLLLNLS
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSALKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEER"
     gene            7807..8112
                     /locus_tag="WJK66_000390"
     CDS             7807..8112
                     /locus_tag="WJK66_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578154.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEHVQNPKGNAQKDGSDAGIRPH"
     gene            8174..9550
                     /gene="mpl"
                     /locus_tag="WJK66_000391"
     CDS             8174..9550
                     /gene="mpl"
                     /locus_tag="WJK66_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEZ7578155.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFKVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFKADVYVIGNVAKRGMDVVEAILNRGLPYISGPQWLSENVL
                     RHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFSVSARLPQTPRQDPN
                     SKSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIICNGQQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     TAGRVKWDLMGRHNRMNALAVIAAARHVGVDIQTACEALGAFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRIGGARILAVLEPRSNTMKLGTMKSALPESLKEADQ
                     VFCYAGGVDWNVAEALAPLGGRLHVGKDFDAFVAEIVKNAEAGDHILVMSNGGFGGIH
                     TKLLDALR"
     gene            9757..10809
                     /gene="bioB"
                     /locus_tag="WJK66_000392"
     CDS             9757..10809
                     /gene="bioB"
                     /locus_tag="WJK66_000392"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEZ7578156.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLDLIYQAA
                     EIHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRQHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFEPE
                     GEVAEVEKASGIKVDY"
     gene            complement(10887..11522)
                     /locus_tag="WJK66_000393"
     CDS             complement(10887..11522)
                     /locus_tag="WJK66_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ7578157.1"
                     /translation="MNIKYSGIENRFETAILKKNGVRSGFVSFAPNLAAVKLNDYAKV
                     KKLIAKTKQKAEIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGAD
                     VVFGQGPHVTRAVELYRDRFISYSGGNFATYGAINTSGISGIAPIFKIITDKQGKFIS
                     GTIIPITQADDKIPKIDPEKTVIGRIIYLNRSDFPKGNGLDVSPDGSITRR"
     gene            11845..12105
                     /locus_tag="WJK66_000394"
     CDS             11845..12105
                     /locus_tag="WJK66_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ7578158.1"
                     /translation="MTKAVRRKNVQPPKICYAFRTPSAYGQYLADAGFDYLSFANNHS
                     NDFGAQGITATAASGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            complement(12168..14027)
                     /gene="ilvD"
                     /locus_tag="WJK66_000395"
     CDS             complement(12168..14027)
                     /gene="ilvD"
                     /locus_tag="WJK66_000395"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEZ7578159.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNISNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHVGRKELFLEAGRMIVEITKRYYEQNDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEKWDVTNPENTHAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEDAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIRLVISDE
                     ELAARRAEMEARGSKAWKPENRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(14200..15969)
                     /gene="cysI"
                     /locus_tag="WJK66_000396"
     CDS             complement(14200..15969)
                     /gene="cysI"
                     /locus_tag="WJK66_000396"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HEZ7578160.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARKNSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAISDGNGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPIRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVPEGGKARIEQLARSYGLIHDGVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIGELDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVG
                     RYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDFAIRTGI
                     VKPVLNAPVDFWDASKAVAIARA"
     gene            complement(15996..17810)
                     /locus_tag="WJK66_000397"
     CDS             complement(15996..17810)
                     /locus_tag="WJK66_000397"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HEZ7578161.1"
                     /translation="MSEHDMQNINPPLPPMPPEITQLLSGLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPAAEPFSVTVLSASQTGNAKSVADKAADSLKAAGIQVRRAELKD
                     YKAKNIADERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCQAGKDFDRRFEELGAKRLLERVDADLDFTASANAWTDNIAALLKEEAAKNRATP
                     APQAMPPADLQTVPEGRYCKADPFPAALLANQKITARQSDKDVRHIELDLSGSDLHYL
                     PGDALGVWFDNDPALVREILDLLGIDPATEIQAGGKTLPVASALLSHFELTQNTPAFV
                     KGYAPFADDDELDRIAADNAVLQGFVQSTPIADVLHRFPAKLTAEQFAGLLRPLAPRL
                     YSISSSQAEVGDEVHLTVGVVRFEHEGRARTGGASGFLADCVEEDGTVRVFVERNDGF
                     RLPEDKSKPIVMIGSGTGIAPFRAFLQQRAAENATGKNWLFFGNPHFAADFLYQTEWQ
                     QFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAKD
                     VEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            complement(17807..19093)
                     /locus_tag="WJK66_000398"
     CDS             complement(17807..19093)
                     /locus_tag="WJK66_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HEZ7578162.1"
                     /translation="MTAQHQTPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKEAIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLNCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAEQLGLKDVHFVPVSALLGDNIVYPGGNMPWYKGEPLLSIL
                     ENLPGEDEVSRTADDFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVEQTVRIEPNG
                     LTAKVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARRYLLKHGTQTLPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            complement(19132..20055)
                     /gene="cysD"
                     /locus_tag="WJK66_000399"
     CDS             complement(19132..20055)
                     /gene="cysD"
                     /locus_tag="WJK66_000399"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEZ7578163.1"
                     /translation="MTKTEPNNAQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTLLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPTEIIRET
                     AVADISERSATRLDDQASEAAMEKRKKEGYF"
     gene            complement(20093..20833)
                     /locus_tag="WJK66_000400"
     CDS             complement(20093..20833)
                     /locus_tag="WJK66_000400"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEZ7578164.1"
                     /translation="METTLFKPALWQIPHIGSGGETALTEKTETLKQRLHRIVGSHRD
                     ARFASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHTETLNLLDRLGRAYPHLRIKRF
                     RPVREDADRYVESKGRFAFYDSVEARRECCRIRKIEPLNRAIAGADAWLTGQRREQSA
                     TRTELPFAEYDAGRGIGKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCT
                     RPVKAGEDIRAGRWWWEGRNSKECGLHK"
     gene            complement(20844..22295)
                     /gene="cobA"
                     /locus_tag="WJK66_000401"
     CDS             complement(20844..22295)
                     /gene="cobA"
                     /locus_tag="WJK66_000401"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ7578165.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGAVAARKISLLLKAGAEVRVAAKH
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VDDRDHCSFIFPSVIDRNPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAVKGKLKSVTERRRFWEKQFNGRFATLAKNRQNTLAERELAGQLEQSRQNDQGGS
                     VSLVGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGE
                     RTPQEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAA
                     VGATAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALI
                     AERLQQHGRSPDTPAAVISQGTLPAQKTATGTLANLAELAETAPNPALIVIGEVVGLH
                     EKLAWFGENGEGENRVGQAYPALGGLNAGQRAA"
BASE COUNT         4846 a         5822 c         6469 g         5328 t
ORIGIN      
        1 tgttaattca ctatataaaa tcggcatttc ggatgtaagc aattttgaag gcagaatgcg
       61 ccatttggaa aacaacggct atgcgaacgt tgccggattg gaacgcatcc tcgccgtcaa
      121 aaccgacaat tacaaagaaa aagaaaacct gctccatgaa attttcagca aaagcaggat
      181 aggcgatacc gaattgttcg ccgtggacga aaaccttgta aaacgtttgt ttttatcgct
      241 tcgcggcgaa atcgtgtttc cgaaaaacga aacggcggaa tcggaatttg aaaaaagcgt
      301 ccacgaacgc aggcaggaag ggaatgccgg gtccggccgc aaacaactgc ttgatttggt
      361 acggcgcgga caccgggaat acccttacgc gctgccccgg cttttggcgg gcgcggcatc
      421 ctacaagccg aaaaaatcga aaatccgcct ttttaaagaa gcatatttcg gcaaaagcgg
      481 cacgaggctg accgacgaaa ttgcagacgg catccatatt tacacctgtt tttcgcgggc
      541 ggatttggaa aaagcctatt ccgaatattt ggaacttttc aaatccgaat cggatgccga
      601 aggcagaaag ccgcagtaag gtgcaaacag ataccgtaca cgttgaggag cagatatgat
      661 gggcgattcc gtcatttatt atgtagaaca ggcagacaaa ccggtaaacc gtgccggcga
      721 acgcgcccgt aaaacattca aatatttttg gcgcgagctt ttttgggaac gccgccgcat
      781 cattcccgcc ttagattttg ccatggtcaa agcccctttt ttccaagacg gcgaagacgg
      841 cgaaatttgc gaacatatgt ggatcgacga tatttatttt gacggtcttt acatttatgg
      901 cgtgctgaac aatgaacccg gcgaactgac caatgtcgaa caaggcgaaa gcgtttgcgt
      961 tccggttgac gacatcagcg actggatgtt cgtgtgcaac ggcattccct acggcggctt
     1021 taccatacag gcaatgcgtg gacagatgac ggaagaggag cgtaccgaac acgatgccgc
     1081 atggggaatc gatttcggcg atcctgggca ggtattgctg gtgtatgaag aaaaagaaca
     1141 tcccgaaaat ctggaagagc atccgatgtg ccggaactgt attgacgatt ttcggcaaca
     1201 gttgtcccaa aatccggatt ttctgcatga gcaggacgaa gacggctata cgccgcttca
     1261 tcatgaagcc atcgcaggaa atgcacttat ggttcaagcc atgcttgaat acggcgcaaa
     1321 tcctgcctca aagacatcgg aaggctatac cgccctcgat tttgcccgcc tggcgggctg
     1381 gcaaaatgtt gccgacctgc tcgaaccgcg acattaggcg gacagttttc cgaaaacgaa
     1441 cacaaacact ttttacagaa agacaataaa aatgcccaaa atcaccgtac ttccacacac
     1501 gacattatgc cccgaaggcg cggttatcga agacgcgccc gaaggcaaaa ccgtccttga
     1561 cgtgctgctc gaccatgata tcgaagtcga tcacgcctgc gaaaaatcct gcgcctgcac
     1621 aacctgccac gtgattatcc gcaaaggttt cgacagcctg gaagagccga ccgaattgga
     1681 agaagacctg ctcgatcagg cttggggttt ggaagccgat tcgcgcctga gttgtcaggc
     1741 ggttgtcgcc ggcgaggatt tgattgtgga aatccccaaa tacaccatca accacgcgcg
     1801 agaagaacac tgaaaacagg ccgtctgaag ccggcacgct tcagacggca ttgttgcgcg
     1861 gataaggcgc aatcgcccga aaacaggcgt tcgtacaggc ggaactttcg attctatagt
     1921 gaattaacaa aaatcaggac aaggcggcga gccgcagaca gtaccaatag tacggcaagg
     1981 cgaggcaacg ctgtaccggt ttaaatttaa ttcactatat attgattttt atcggttttc
     2041 tgacggaacg gattggtgcg gcatccgagg cggattactc gggcgcgatg cacgggtatt
     2101 tatcggtttt gcagccggaa aaaccgccgg cgggttactt tcagggcggc ggtcgggcgg
     2161 tatgccggat agcgttccac tttcgcttct atattgattt tgattggttt tctgacggaa
     2221 tgatccgatg cggcatccgg gacggcttgt gtttcttcct gcccgcctgc cggatattcc
     2281 tgtacttgca cgaaaccgaa agaggtggcg gtgcggcgga caagctcgag atagcgtcct
     2341 tcaagctccg gacaggcggc ggacacgctt tctgccgtaa ccgtgaagcc gccgcccgac
     2401 ccggcaaggc gttccgcaat ctgcgtgtag atgcgccaac gttgggcgcg caacattgcc
     2461 gcatcgcaac cggcagccgc tttatccaag gcaatcagac cgcgcctgct ccggtagtga
     2521 aggcaaaccg ttactctgga gaggatatgt tgcccgtcca atatttgata cagtttgcgt
     2581 atcagcagaa tcaggcggtc aaactcctcc atctccgaca gggaatcagg atggtcggaa
     2641 gcggtataaa ccagtttgga caattttgcg gcaaacatat tgttcatcaa tcttccttgt
     2701 cggttgaaac cccgctcttc ggggcggtag aatcagattt gtttgggagg gacaactcct
     2761 cccagatcag gacgacacat aggctggtgc ttgatgtgtt gtccggcgag ttgaaacatt
     2821 cagccatcct caaggtgcgg cagttttgcc gaaacatatt ctacacggct tcaatgccgg
     2881 acgataaaag gaaattcata tgaaatggac cgacacccgg cgtatcgccg aagaactcta
     2941 tgacctgcac ggcgaaacca tcgatcccag aaccgtgcgc tttacccaac tgcgcgacct
     3001 gattatggca ttgcccgaat ttgacgacga ccccgcccgt tgcggcgaac gcatcctcga
     3061 agccgtgcag caggcatgga tagacgaggc ggaataagtt tcgggaatgc cgtctgaaat
     3121 gcggcggtac gcggttcgtg cttctgtttg cagcgggaat ggttttacca gtctcctttt
     3181 ttcagcctgt ccagttggcg gcggtcgcgc ttggtcggtc tgccgtcggg ataggcggaa
     3241 gtgatgcggc tgaattggtc gagctgtttg cgctcttccc tcaatgttgc cgttttcgcg
     3301 tcctcttcat acagaagccg cgcctcggat gccgggcggc gttggtggtt caaaccttta
     3361 accttgattt tatagggaag ggaattgagc gtcaggtcga taatatcgcc gatgtctatg
     3421 gttttactgt ttttgacctt cgagccgttt acttgaaccc tacccagttc gatgtgcttt
     3481 tgcgcaaggg aacgggtctt gaaaaaacgt gccgcccaaa gccatttgtc cagccgcatg
     3541 gcggaagaat cgtgcttgtc tttcatacga ttttgtttga aataattgaa tttgtttcga
     3601 gtttagcata agatacgccg cctataacta gtatatatgc actaatccac tgttttccat
     3661 gctgtccgaa cacaaaaaga gggtgtggaa aagccgtttt ggacaataaa ttaactgcgg
     3721 aatatgcaca aatagcgtat gatagcggca gaatctgttg atgagagctt cattctatga
     3781 aacctgtttt tttggatttt gaacaaccca tagccgaact gaccaacaaa atcgatgagc
     3841 tgcgtttcgt ccaagacgag tctgccgtcg atatttcgga cgaaatacac cgtttgcaga
     3901 aaaaaagcaa cgacctgacc aaatcgattt tcagcaaact tacgcccgcc caggtttcac
     3961 aggtttcccg gcatccgcag cgtccctata ctttggatta cattgatgcg ctgtttactg
     4021 attttgaaga gctgcacggc gaccgccatt ttgccgacga tcatgcgatt gtcggcggat
     4081 tggcgcgttt caacggacaa agcgtgatgg tcgtcgggca tcagaaaggg cgcgacacca
     4141 aagaaaaaat ccgccgcaac ttcggtatgc cgcgccccga gggctaccgc aaagccctgc
     4201 gcctgatgaa aacggctgag aaattcggct tgcccgtcat gacctttatc gatacgccgg
     4261 gcgcgtatcc cggcatcggc gcggaagaac gcgggcagtc ggaagccatc ggcaaaaacc
     4321 tgtacgaact gacgcgcctg cacgtccccg ttttgtgtac cgtcatcggc gaagggggct
     4381 cgggcggtgc gttggcggtc gccgtaggcg attacgtcaa tatgctgcaa tactcgacct
     4441 attctgttat ctcccccgaa ggctgcgctt cgattttgtg gaaaaccgcc gaaaaggcgg
     4501 cggatgcggc tcaggctttg ggcattactg ctgaccgcct gcaaaagctg gacttggtcg
     4561 ataccgtcat caaagaacca ttgggcggcg cgcatcggga tttcgggcaa accatgaaaa
     4621 acgtaaaagc cgttttggaa aaacaactgc acgaagcgca aagcatcccg cttgccgatt
     4681 tgctttcgcg ccgtttcgac cgcattatgg cttacggcaa attttcggaa caataattca
     4741 ggtagaacaa gcagcaagca gtttgtctga aactgcttgc tttttcttta tcgggacgga
     4801 accgtgctga ctttagatgc gtttgagcaa tgcttgaagg attgttttcc tcaaggtctg
     4861 aatggaaaaa aaacagcagt ggcattaagc ggcggcttgg attccgtcgt tttgctgcat
     4921 ctgcttgtcc gcgccggaaa aaagggcggt tttattccgg atgcattgca tatccatcac
     4981 ggcttgagtc cccgtgccga cgattgggca gatttctgcc aaaactattg cgatatgctc
     5041 ggggtggggc tggaaacggt taaggtctgc gtggaaaaaa acggtttggg catcgaggcg
     5101 gcggcgcggc aaaagcgtta tgccgcgttt gccgaaaaag gctttgacgt tttggcgttg
     5161 gcgcaccaca gggacgatca aatcgaaacc tttatgctgg cagtcgctcg cggcggcggt
     5221 ttgcgcgctt tggcggctat gcccaccgtc cgcccttttg gggaaaaagg catcatctgg
     5281 cggcctttgc tgcctttttc gcgccaagac atatgggatt atgcccaaaa acacggtttg
     5341 ccgaatatcg aggacgaaag caatagcgat acggcttatt tgcgaaaccg cttccggcac
     5401 cgtattttgc ccgaactttc ggcgcagatt ccccatttcg ggcggcatgt actgaacaat
     5461 gtccgcgctt tgcaggaaga tttggctttg ttggacgaaa tcatcggtca ggactgccgt
     5521 tgggtctgtg aggaaggcag tttcgatacg gtgcgttggc aggcgttttc cccacgccgg
     5581 aaaacccata ttttgcggta ttttttgaag gaaaacggca tccccatgcc gaatcagaat
     5641 gcccttgccg acattgcccg ggttttgacg gaggcaaaaa ccggacgttg gaacttgcaa
     5701 ggctttgaat tgcatcatta tgcaggcagg ctgtttgtgt tccgactgga aaaaacggat
     5761 aaactgcggt ttttgaaaga caggcagata agcggaaatt taagggaaat attgacgggg
     5821 cagggatttg ttttaaaacg tcatccgttt gggcttcctg agcatctttt ggagcaggac
     5881 ggaattttga ggacggtagc ggtatcggat acgttggctg tcggcggcat ccataaggat
     5941 gtgaaaaaaa tccttcaggg gaaacgggtt ttgcctgtcc tgcgcccaat ttggccgctt
     6001 gttgccgaca gcggaaaccg tccattggcg ttggcaaact gttgtgcgga ttttcaaatc
     6061 tcggtttcag acggcatttt gcccgtccat cctgactttc ccattttatt ttgataatat
     6121 cgcaaacaga tttcggcggc gttcagtcgg gtattgtccg gttgcatatt tctaaaaggc
     6181 ttgtgaagtg agacacatca gttcgaccaa taatgaacac atcagacacc tgcaccgcct
     6241 gttgtcgcaa ggaaagttca gacggcaata cgcccaaacc gttttggagg gcgtgcacct
     6301 gcttcaggtt ttcctgcaat ccggcgggat gccggtcggg gtatatattc ccgagggcaa
     6361 aatgccgtct gaagaaatcc gcaggctgag ggcggttgtg ccggaaggca ggattttttc
     6421 cgtttcagac ggcatattga aaaaaatcag tagcttgagt tgtgcagacg atatacttgc
     6481 actgattgat atcccattgg gcggaacctt gccggacaaa ggcgactgtg tggttttgga
     6541 cggcgtgcaa gatccgggca atgtcggcac cgtcttacgg agtgcggcgg cggcaggggt
     6601 aggtacggtc gttttgggca ggggttgcgc ggacgcgtgg tcgcccaagg tattgcgcgc
     6661 ggggatgggc gcgcatttct tgtcgggcat ttatccgcag gcggatttgg aaatatggtt
     6721 ggcgcgctat aaaggccgtg tgtttgccac cgccttgcgc gaggaaaagc aggcggtttt
     6781 gtacggcgaa gatttgtgcg gaccgacagc ctgggtgttt ggcaacgaag gcgcgggggt
     6841 cggtaaagcg gttttggaca gggcggacaa gtgtgtcagg attccgatgc acgatgcaac
     6901 cgagtcttta aatgtcgcga tggcggcgac aatctgcctg tttgaacaga tgcggcagcg
     6961 gtcggcgtat tgaggaaaag aaatgccgtc tgaaaaaatc tattacggcg tattgatttt
     7021 cttatgtatc gcttctatgc tgctgtcgcc gtttttttat gcgggtgctt tgaagcccaa
     7081 gaaggcggca ttgcggaagg acgggcagtg gaaactcatc ttattgtcca ataccgtggc
     7141 ggcggcggtt ttggcttggg tgtggtggaa atggttttga cagatattgc tcaaaatcgt
     7201 gctaatggaa tccgaacaaa taaagaattt ggtaaaaaat ttgttaaatc aacgaattaa
     7261 agttttgtgg aaaacaaaac agctctaagc aaatagggcg tttgtcggta aatacggaag
     7321 agttgcggca ttatcgggca tctttaacaa gtagtgccgt cttggcaggc aatcggtttt
     7381 tatgggcagc ttgcaaaatc gcggatataa aattgcgaat cggttaaagt gtggggacgc
     7441 tatgaaaaat tgcgaatttt tttatgaccc gacaagggca atctatgata gcggtgcaga
     7501 ttacttaact agggaaaaac atagattagt cgtaattgca aatagtgctt gggggctatt
     7561 acttaattta tcttgttatt atgacgaggt tttggaaaag cggaaaatac cgttcggcaa
     7621 acaggaaatt gatgacgata tggacaaagt gtccgccctt aagcggaagt ttaaagatat
     7681 ttctgaaatc aaagtagggg atggttggga atacccgttc aattatgagc agggaatgaa
     7741 agaattagat gaagtgctat tgaaatacat tccctttttt gaagaagaac gataaaggag
     7801 gttgatatgc gcgtatctaa aataattgga agtatgttgc ttgttacagc ggttcagacc
     7861 gtattttcgg caaatgttta cgcgtgccgc cataatggta aaaccagtta cagccaaact
     7921 ccgggaaaac attgtaccaa cgcgggtttg gggcgggacc gggtgtacag ttcggttaga
     7981 ccggcagtaa aagacagggc ggaagacgca ggggtcggcg attattcgga cacggtgagg
     8041 gacgaacacg tccaaaatcc gaaagggaat gcacagaaag acggttcgga tgccggcatc
     8101 aggccgcatt gattgaaacc gaatcagcca tgacggcgtt gaataataga atttgaacga
     8161 ttagggaact ctgatgaaac acatccacat tatcggtatc ggcggcacgt ttatgggcgg
     8221 gattgccgcc attgccaaag aagcggggtt taaagtcagc ggttgcgacg cgaagatgta
     8281 tccgccgatg agcacccagc tcgaagcctt gggcataggc gtacacgaag gcttcgacgc
     8341 ggcgcagttg gaggaattta aagccgacgt ttacgtcatc ggcaatgtcg ccaagcgcgg
     8401 gatggatgtg gttgaggcga ttttgaaccg cgggctgcct tatatttccg gtccgcaatg
     8461 gctgtcggaa aacgtgctgc gccatcattg ggtactcggt gtggcgggga cgcacggcaa
     8521 aacgaccacc gcgtccatgc tcgcatgggt tttggaatat gccggactcg cgccgggctt
     8581 ccttatcggc ggcgtaccgg aaaactttag cgtttccgcc cgcctgccgc aaacgccgcg
     8641 tcaagacccg aacagcaaat cgccgttttt cgtcatcgaa gccgacgaat acgacaccgc
     8701 ctttttcgac aaacgctcca aattcgtgca ttaccgtcca cgtaccgccg tgttgaacaa
     8761 tctggaattc gaccatgccg acatctttgc cgatttgggc gcgatacaga cccagttcca
     8821 tcacctcgtg cgcaccgtgc cgtctgaagg cttaatcatc tgtaacggac agcagcaaag
     8881 cttgcaggat actttagaca aaggctgctg gacgccggtg gagaaattcg gcacggaaca
     8941 cggctggcag gccggcgaag ccaatgccga cggctcgttc gacgtgttac tagacggcaa
     9001 aaccgccgga cgcgtcaaat gggatttgat gggcaggcac aaccgcatga acgcgctcgc
     9061 cgtcattgcc gccgcgcgtc atgtcggtgt cgatattcag accgcctgcg aagccttggg
     9121 cgcgtttaaa aacgtcaaac gccgcatgga aatcaaaggc acggcaaacg gcattaccgt
     9181 ttacgacgac tttgcccacc atccgaccgc catcgaaacc acgattcaag gtttgcgcca
     9241 acgtatcggc ggcgcgcgta tcctcgccgt cctcgagccg cgttccaaca cgatgaagct
     9301 gggcacgatg aagtccgccc tgcccgaaag cctcaaagaa gccgaccaag tgttctgcta
     9361 cgccggtggc gtggactgga acgtcgccga agccctcgcg cctttgggcg gcaggctgca
     9421 cgtcggcaaa gacttcgatg ccttcgttgc cgaaatcgtg aaaaacgccg aagcaggcga
     9481 ccatattttg gtgatgagca acggcggttt cggcggaata cacaccaaac tgctggacgc
     9541 tttgagatag cccgggcgat gccgtctgaa agcccttcag acggcatcgc ccggctgcgc
     9601 ggcacaaagg cggaaaaacc gtttgccccg tattttcaaa cgcgttacac ttgccgccgc
     9661 tgttttcagc catttgatta cccgcaaccg ccgtcattgc gccggcggtt tgcctgtcag
     9721 cgtcattgcg ccgctgtaaa tacgaaagaa cacattatga ccgtatcccc cgtcgccttg
     9781 cgccgtaaga ccgagtgcaa gcctcatccc accgcgcgct attggaaaaa atgcgatgtc
     9841 gaagccctgt tcggacttcc cttcctcgac ctcatttacc aagccgccga aatccaccgc
     9901 caaaatttca acccgcgcga aatccagctt tccacgctgt tgtccatcaa aaccggcggt
     9961 tgtcccgaag actgcgccta ttgtccgcaa tcggcgcacc acaataccaa tctgggcaaa
    10021 gagcagatga tggatgtgga tgaaatcgtc gaaaaagcca aaatcgccaa atcgcgcggc
    10081 gcaagccggt tttgtatggg cgcggcgtgg cgcggcccta aacccaaaga cgtggagacg
    10141 gtttccgcaa tcatcaaagc cgtcaagggc ttgggtatgg aaacctgcgg cacgttcggt
    10201 atgctcgaag aaggtatggc ggaagacttg aaagaggcgg gcttggatta ttacaaccac
    10261 aacctcgaca ccgaccccga ccgctacaac gacatcatcc acacccgcca acacgaagac
    10321 cgaatggaca ccttgggcaa agtccgcaac gccggtttga aagtctgctg cggcggcatc
    10381 gtcgggatga acgaaacccg cgccgaacgt gccgggctga ttgccagtct cgccaatctc
    10441 gacccgcagc ccgaaagcgt gccgattaac cggttggtca aagtggaagg cacgccgctt
    10501 gccgatgccg aagatttgga ctggacggaa tttgtccgca ccatcgccgt ggcgcggatt
    10561 acgatgccgc aaagttatgt ccggctgtcg gcagggcgca gcaatatgcc tgaagcaatg
    10621 caggcgatgt gctttatggc gggcgcgaac tcgatttttt acggcgacaa gctgctgacc
    10681 acgggcaatc ctgatgagga cggcgacaga atcctgatgg aaaagctcaa cctgtatccc
    10741 ttgcagtttg aaccggaagg cgaggtcgcc gaagtggaaa aagcctctgg gattaaagtg
    10801 gattattgac gattgaaaaa tgccgtctga aacccggaaa aaggctttca gacggcattt
    10861 gtccggacgg catttccaat atctttttac cggcgcgtga tgctgccgtc gggcgagaca
    10921 tccagcccgt tccccttggg gaaatcgctg cggttcagat aaataatccg cccgataacg
    10981 gttttctcgg ggtcgatttt ggggatttta tcgtcggctt gggtaatagg gatgatagtt
    11041 ccggaaataa atttaccctg tttgtcggtg ataattttga aaatcggcgc aatgccgctg
    11101 atgccggagg tgttgattgc gccgtaggtg gcaaagttgc cgccgctgta ggagatgaag
    11161 cggtcgcggt aaagttcgac ggcgcgtgta acgtgcggcc cctgcccgaa tacgacatcc
    11221 gcgccggaat cgacggcaag ccgtgcaaac tcgacgacgt tgcccctgtt ttccccatag
    11281 aagatttcgg tatcgaacgg caggtgttcc gcctgtttcc cttccgcgcc gccgtggaac
    11341 atcacaataa cgatctcggc tttttgtttg gttttggcaa tcagtttttt gactttggca
    11401 tagtcgttca gtttgacggc ggcaaggttg ggggcgaagg agacgaagcc ggatcttacg
    11461 ccgtttttct tcaggatggc ggtttcaaac ctgttttcga tgcccgaata tttgatgttc
    11521 aattcgtcaa ggttcgccct ttatgccgtt tccgcaccgc aaaccgccgg ggtcaagccc
    11581 tcggcgcatc ctgccggaac ggaatccccg tgctgccgat tgacggttca aaccccgcgc
    11641 ccgtttcaaa taccggcgat gtggacggac aggatgcgcc tgacgaaaag acagccgata
    11701 ccgtttccat tatcggcgtg ggcgacatta tgctcggcag caattatccg gtcgattacc
    11761 tgcccgatac caatattctg aaaaacgtcg aatctgcctt gcaagacgcg gacattaccg
    11821 tcggcaacct cgaaggcacg ctgtttgacg aaggcggtac gccgaaaaaa tgtgcaaccc
    11881 cccaaaatat gctatgcatt ccgaacgccc tccgcatacg ggcaatacct tgccgacgcg
    11941 ggattcgact acctcagctt cgccaacaac cacagcaacg acttcggcgc gcaaggcatc
    12001 acggcaacgg cggcgagcgg cagctctttt acatactcga tcgcgctaaa gccgctgccg
    12061 ataacgaggc caaaattgcg gaaaataccg ccatcgccca gataaatttg tccatcatca
    12121 gacctttact gttcagacga gacagcattt gccgcacgtt ttggggctta tctttcgatt
    12181 tgcgctacgt cgcgcaccgc gcctttgtcg gcggaagtcg ccatcgcgcc gtaagctctt
    12241 aatgcggcgg agacgtagcg gtcgcggttt tccggtttcc acgctttgct gccgcgtgct
    12301 tccatttcgg cacggcgggc ggcgagttct tcgtcggaaa tgacaaggcg gatgctgcgg
    12361 ttggggatgt cgatttcgac ggtatcgcct tcgtgtacca aaccgatcgc gccaccttcc
    12421 gccgcttcgg gcgaggcgtg tccgatggac aaacctgatg tgccgccgga gaagcgtccg
    12481 tcggttaaga gagcgcaggc tttgccgagg cctttagatt tcaggtagga agtcggatac
    12541 agcatttcct gcatgcccgg gccgcctttc gggccttcgt agcggatgat gacgatgtcg
    12601 ccggcgacga tttggttgcc caaaatgcct tctactgcgt cttcttggct ttcaaacacg
    12661 cgggcgcggc cggtgaattt gaggatgctc tcgtccacgc ctgcggtttt taccacgcag
    12721 ccgcgctcgg cgatgttgcc gaacaagacc gccaaaccgc cgtcttgcga gtaggcgtgt
    12781 gccacgtcgc ggatacagcc tttttcgcgg tcgaggtcga gggttttcca catacggttt
    12841 tgcgagaacg cttgggtggt gcgtacgccg ccgggcgcgg ctttgaagcg ttcgatggcg
    12901 tgggtgtttt cagggttggt tacgtcccat ttttcaatcg cgtctttcag cgtcggcgcg
    12961 tggatagtgt gcacgtcggt gtgcagcttg cccgctttgt ccaattcttt caggatggcg
    13021 aagatgccgc cggctcggtg cacgtcttcc atatagtagt cgtggttgtt gggtgcggtt
    13081 ttgcagatgc agggcacaac gcggcttaag cggtcgatgt ctgccatttt gaaatccacg
    13141 cccgcttcgt tggcaacggc gagtaaatgc aaaatagtgt tggtgctgcc gcccatggca
    13201 atgtccatgg tcatagcgtt ttcaaacgct tttttggtgg caatgctgcg cggtaacacg
    13261 gtttcatcgt tttgctcgta atagcgtttg gtgatttcga caatcatacg gccggcttcg
    13321 aggaacagtt ctttgcggcc gacgtgggtg gcgaggtaag aaccgttgcc cggcagggac
    13381 agcccgagtg cttcggtcag gcagttcatc gagtttgccg taaacatacc cgaacacgag
    13441 ccgcaggtcg ggcaggcgtt ttgttcgact tcttcaactt gccggttgct gatattgtcg
    13501 tccgccgatt caatcatcgc gtcaatcaag tccaaacggc gttcgggctg gatgtttgcc
    13561 acgccgataa ctttacctgc ttccatcgga ccgccggaaa cgaagatggt cgggatgttc
    13621 aggcgcatcg cggcaatcag catgcccggg gtgattttgt cgcagttgga aatgcacacc
    13681 agcgcgtcgg cgcagtgggc attgaccata tattcgatgg agtcggcaat caaatcgcgg
    13741 ctgggcaggg aatacagcat accgctgtgt cccatagcga tgccgtcgtc gatggcgatg
    13801 gtgttgaatt ctttggcgat ggcgccggct ttttcgattt cgcgggcaac cagctggccc
    13861 atattgtgca ggtggacatg acctggcacg aattgggtga acgagttggc aacggcgata
    13921 atcggtttgc cgaagtcggt ttccatcacg ccggtggcgc gccacaacgc gcgcgcgccc
    13981 gccatattgc gaccgtgggt ggaggttttg gagcggtatt ctggcatagt gtgtttcctt
    14041 gtgcctatac cgtctgaaag acagggctgt ttcagacggt atcgggtacg gttttttaga
    14101 gtgggaaaag agggtatttt ataccaagta tcggaatttt gcgggattga aacggcgtgc
    14161 ggcaaaaaag aaaatccccg caggaatgcg gggacgggtt caggcgcggg caatcgcgac
    14221 ggctttggat gcgtcccaaa aatcaacggg tgcatttaat acgggtttga cgatgcccgt
    14281 ccgtatggcg aaatcgccga aaccttcgcc gatattgcgt tctgccgccc atttgccgat
    14341 caggtcgtcc aattcggcaa ggatttcggg cagggtgatg ttttctttgt aaagacgggg
    14401 gatgcgtacg ccttcacggt cgccgccgat atggaggttg tagcgtccga cggctttgcc
    14461 gaccagtccg atttccgcca acatcgcccg tccgcagccg ttcgggcagc cggtaatgcg
    14521 ggtaacgatg tagtcgtccg acgtgccgtg tttcgccata atcttatcca gctcgccgat
    14581 gaagtccggc agcacgcgtt cggcttccgc cattgccagc gggcaggtcg ggaaggaaac
    14641 gcacgacatc gcattttcgc gcagtttgct gacaccgtcg tgtatcagcc cgtatgagcg
    14701 ggcgagctgt tcgatccggg ctttgcctcc ttcgggtaca tttgccacga tgaggttttg
    14761 gttggcggtg atgcggaagt cgcctttgtg gattttggcg atttccaaca cgccggtcag
    14821 aagctgtttc ccgccttcgt caaccaaacg cccgctttcg atgaaaaggg tcaaatgcca
    14881 gttgccgtct atgcctttca cccagccgat gcggtcgccg cgcccggtaa atttgaacgg
    14941 gcgtatgggt tcgaacggca tacccatacg gcgttcgact tccgcgcgga agttgtccaa
    15001 gcccatattt tgaatggtgt agcgggtgcg ggcgtttttg cggtcgctgc ggttgccgaa
    15061 gtcgcgctgt gtggttacca ccgcttcggc ggccttcagc gcgtgttccg gaggcacgaa
    15121 acccagttcc agtgaaatgt tcggataggt tttggtgttg ccgtgttcca tcgaaagccc
    15181 gccgcccgcc aaaacattga atccggcaag ctgtccgttg ccgtctgaaa tggcgacaaa
    15241 atccaagtcg ttgccgtagc agtccacatc atttaagggc gggatgacga ctgcggtttt
    15301 gaatttgcgc ggcagatagg ttttgcccaa aatcggctcg tcttcttgaa ggaagtcgtc
    15361 ggaactttga acttttttgc cgtccaccca cacatccaga taaccgcgcg tgcgcggcag
    15421 caggtgttcg gaaatctttt tcgcgtattc gtaagcctgc cggtgcagtt cggactcgat
    15481 cgggttggac gtgcaaagca cgttgcggtt catatccgcc gccgtggcga tggaatccaa
    15541 acccagtttg tgcaagaggc ggtgcatcgt ctgcaacttg gctttcggca cgccgtgaaa
    15601 ttggaaggtt tgccggttgg tcagccggat ggagcggtaa tgactgtttt tccgggcaaa
    15661 tttgtccagt tctatccatt gggacggttt gatgatcccg cccggcagcc ggcagcgcaa
    15721 aagcataaat ttcaagggct cgagttttgc ctcggcgcgt tcggcgcgga tgtcgcggtc
    15781 gtcctgctca tacataccgt ggaagcggat gagttggaag ttgtcgcctt tgaagccgcc
    15841 cgtgagcggg tctttcaaat cgtccaaaat cgtgccgcgt aaaaaattgc tttcggtttt
    15901 cagacgttcg ttgtcggata gcggtttttc ttgccacgcc aaaccttttg tcttggtctg
    15961 tacggtcatt ttgtgttcct cccgattata tttaatcaat aaacatcacg ctgatagcgt
    16021 ttttcttcgc gcaacatatc caaatattct tctgcgccct cttcgtccaa atgtcctgcc
    16081 ccgataatca catccagcaa ggcggcttcc acgtcttttg ccatttttgc cgcatcgccg
    16141 cacacataga tatgcgcgcc ttcctgcagc cattgccaaa gtccttccgc ctgttcgcgg
    16201 attttgtcct gcacatagat tttttcttcc tgatcgcgcg accaggcgaa atcgtatctg
    16261 tgcaggaagc cgtctttggc aaactgctgc cattcggtct gatagaggaa gtcggcggca
    16321 aaatgcggat tgccgaaaaa cagccagttt ttgcctgttg cattttctgc ggcgcgttgt
    16381 tgcaaaaaag cgcgaaacgg tgcaatgccc gtaccggagc ctatcattac aatgggtttg
    16441 cttttgtctt cgggcagtct gaagccgtcg ttgcgctcca caaatacacg caccgtgccg
    16501 tcttcttcga cgcaatcggc aagaaaaccg gatgcgccgc ccgttctggc gcggccttcg
    16561 tgttcaaaac gaaccacgcc gacggtcagg tgcacttcgt cccccacttc cgcctgcgac
    16621 gaggaaatcg aatacaggcg cggcgctagc gggcgcagca gaccggcgaa ttgttccgcc
    16681 gtcagttttg ccgggaagcg gtgcagcaca tcggcaatcg gcgtgctttg cacaaagcct
    16741 tgcaaaacgg cgttgtcggc agcaatacgg tcgagttcgt catcatcggc gaacggggca
    16801 tagcctttga caaaggcagg ggtgttttgc gtgagttcaa aatgggataa cagtgcggag
    16861 gcaaccggca gggtttttcc gcccgcctgt atttccgttg ccggatcgat gccgagcagg
    16921 tctaggattt ccctgaccag tgccggatcg ttgtcaaacc aaacgccgag cgcgtcgccc
    16981 gggaggtagt gcaaatccga accgctcaaa tcaagttcga tgtggcgcac gtctttatcg
    17041 gattggcggg cggtgatttt ctgattggcc agcagggcgg cgggaaaggg gtctgccttg
    17101 cagtacctgc cctccggtac cgtctgaagg tcggcggggg gcattgcctg tggcgcgggt
    17161 gttgcccggt tttttgcggc ttcttctttt aagagtgcgg cgatattgtc tgtccaggcg
    17221 tttgcagagg cggtaaagtc caaatccgca tcaacgcgtt cgagcaggcg tttcgccccc
    17281 aattcttcaa aacgccggtc gaaatcttta cctgcctgac agaaattcgg ataggagctg
    17341 tcgcccaagc ccagtacggc aaattggagt ttgtccaatt tcggggcttt tttgccgttc
    17401 agcagtttgt gcagcacgac ggcttcttcc ggcggttcgc cttcgccctg ggtggacgta
    17461 accagcagca ggcggcgttc gtctgcgatg tttttcgcct tgtagtcttt cagttcggcg
    17521 cgcctgactt gtatgccggc ggctttcagg ctgtccgccg ctttgtcggc aacggatttc
    17581 gcattgccgg tttgcgaggc ggaaaggacg gtaacggaaa agggttctgc cgccggcaat
    17641 gctgtctgaa gcgcgggcag ccctgcggat gccccgtttc ctgcttttgc ccaagcatag
    17701 ccggacagcc acgcccattg tgccgcgtcc agccccgaca ggagctgcgt gatttcgggc
    17761 ggcataggcg gtaatggcgg atttatgttc tgcatatcgt gttcactcat aaaatcatac
    17821 ctgccgcaac agtgccgtat gtcgcttcgt ctatcaggat aaacgaaccg gcggcggtat
    17881 tttccgcata aggcgttgcc gtaacgggtt tttgaaggtt gatgcggact ttggcgatgt
    17941 cgttcatctt caaggattcc gcgccggcct cttgttccag cgtgcggaca tccaaaacgc
    18001 tttcaatttc cccgactttt gccggcaggg tttgcgtgcc gtgcttgagc aggtatctgc
    18061 gcgcggtatt gagcggacgt tcgtcaaacc agcaaagcgt ggcttccaga tgtttttgcg
    18121 gggcgagcgg ggaattttta tcgacaaaaa ggtcgccgcg cgaaacatcg atgtcgcgat
    18181 ccagccggag ggttgccgcc tcgccggcaa aagcctgcgc cacttcgcct ttgggtgtga
    18241 tgatttcaga cactttggcg gtcagcccgt tcggttcgat gcggacggtt tgttcgacgg
    18301 cgaccgaacc gcgttcgatg cgcccctgat agcctcggaa atcatcggcc ttgtcggcat
    18361 cttggcggac gaccagttgc acggggaaat aaaaatcgtc ggcggtgcgg ctgacttcgt
    18421 cctcccccgg caggttttcc aaaatggaca ataagggttc gcctttatac caaggcatat
    18481 tgccgccggg gtaaacgatg ttgtcgccca agagtgcgga caccggtacg aaatgcacgt
    18541 ctttcaaacc gagctgttcg gcaagtcggc ggtatgcctc cacaatggcg ttgaatttgt
    18601 cttcgctgta atccagcaga tccattttgt tgaccgccac cacaatatgc gggcagttga
    18661 gttggcggag gatggcggaa tggcgtttgg tctgcggcag aagctgcaag ggctgcgcgc
    18721 cgaaatccag ttgggatgcg tcaaccagta cgactgccgc cgaagcggtg cttgcgcccg
    18781 taaccatatt gcgcgtgtat tgttcgtgcc ccggcgtgtc ggcgatgatg aatttccgtt
    18841 tcgccgtgga aaaatagcgg tatgccacat cgatcgtaat gccctgttcg cgttcggctt
    18901 ccagtccgtc ggtcaggatg gagaagtcta tggcttcttt caaacctttg cttttgccgg
    18961 attccaaggt tttgatttgg tcggacagca gggctttgct gtcgtagagc agtcgtccga
    19021 ttaaggtgct tttgccgtca tcgacgctgc cggcggtaat gaagcggagc ggggtctggt
    19081 gttgtgccgt catattttct tcctcatatc tgcttaaagg gttcttgaaa tttagaaata
    19141 gccttctttt ttgcgttttt ccattgccgc ctcgcttgcc tgatcgtcca gccgggtcgc
    19201 gctgcgttcg gaaatgtcgg caaccgctgt ttctctgata atctccgtcg gcgtggacgc
    19261 ggtgctttct accgggcagg tgcagctgat gtcgccgacg gtgcggaagc ggacatcaag
    19321 gatttcggag gtttcagacg gcattttcgg ggtgagcagc gttacgggga ccagcagccc
    19381 cctgcgtctg accacttcgc gcctgtggct gtaataaatc ggcggcagct cgaggttttc
    19441 gcgggcgatg tattgccaaa tgtcgagttc cgtccagttg gaaatcggga agacgcgcat
    19501 attttcgcct ttgtgcagcc tggtgttgta cagcgaccac agctcggggc gttgcgcctt
    19561 cggatcccat tgtccgaact cgtcgcggaa cgagaaaatc cgttctttgg cgcgggcttt
    19621 ttcttcgtcg cgccgcgcgc cgcccataag cgcgtcaaaa ccgtttgcct cgatggtttc
    19681 caacagcgta accgcctgtg ccgcattgcg cgaatcggtt tctttgcgta agaccaccgt
    19741 gcctttggca atggagtctt ccacgcgccc cactatcagg cgggcattga gttttgccgc
    19801 ctgcgcgtcg cggaaggcaa tcacttcggg gtagttgtgc cccgtgtcga tatgcaccag
    19861 cgggaagggc agttttaccg gcctgctgcc cagccggaag gctttgcagg cgagggcgag
    19921 caggaccacg gaatctttgc cgccggaaaa gagcagggcg gggttttcgc attctgccgc
    19981 cacttcgcgg atgatgtgga tggattcgga ttccaaccag tcgagttggg cgttgttcgg
    20041 ttcggttttc gtcataccat attccttatt tcttctgtct gatatttatg aattatttgt
    20101 gcagcccgca ttctttgctg tttctgcctt cccaccacca ccgcccggcg cggatgtctt
    20161 cgcccgcctt gacggggcgg gtgcaggggt cgcagcctat gctgggaaat ccttgccggt
    20221 acaaatcgtt gtaaggcaca ttgttggcga ggatgtatgc ccacacgtcg tgttccgacc
    20281 agtcgaaaat cgggttgtat ttgccgatgc cccgtccggc atcgtattcg gcaaacggca
    20341 gttccgtgcg tgtggcggat tgttcgcggc gttgcccggt aagccaggcg tccgcgcctg
    20401 caatggcgcg gttgagcggt tcgatttttc ggatgcggca gcattcgcgg cgcgcttcaa
    20461 cgctgtcgta aaaggcaaac ctgcctttgc tttccacata acggtcggca tcttctcgaa
    20521 ccggccggaa acgctttatc cgcaaatggg gatatgcgcg tccgagcctg tccagcaggt
    20581 tcagggtttc cgtgtggagc agccccgtat ccaaggtaaa aatgccgata ttgaggtttt
    20641 cgccggcgat aaggtcggta atcaccatat cttctgccgc aaggctgctg gcaaaccgtg
    20701 catcccggtg gctgccgaca atccggtgca ggcgttgttt gagggtttcc gttttttccg
    20761 taagggcggt ttcgccgccg gatccgatat gcggtatctg ccacagggcg ggtttgaaca
    20821 gtgtcgtttc catttttccc gccttatgcc gcccgttgtc cggcattcag tccgcccaat
    20881 gcgggatacg cctgcccgac ccggttttct ccttcgccgt tttcaccgaa ccaggcgagt
    20941 ttttcgtgca gccccaccac ttcgcctatg acaatcaatg ccggattcgg cgcggtttcg
    21001 gcgagttcgg caaggttggc gagcgtgccg gttgcggttt tttgagccgg cagcgtgcct
    21061 tggctgataa cggctgccgg cgtgtcgggc gagcgtccgt gctgttgcag ccgttcggca
    21121 atcagggcgg ctttgagcgc acccatataa atcaccaagg tctggcggct gcgggcgagg
    21181 gtctgccatt cgatgtcggg cgcatccgcc ttgcggtggc cggttacgaa aaccgcactt
    21241 tgggcataat cgcggtgcgt gagcgggatg ccggcatagg cggtcgcgcc gacggcggcg
    21301 gtaatgccgg ggacgaccga aaacggaatc tgatggcgtg ccaaggtttc caattcttcg
    21361 ccgccgcgtc cgaacacgaa agggtctccg cctttcaaac gcaccacgcg cctgccttcg
    21421 cgggccagcc tgaccataag cgcattggtg tcctcttgcg gggtgcgctc gccccgggcg
    21481 cgcttgccga caaaaatccg ttccgcatcg cggcggacga gggacagtat gccgtctgaa
    21541 accagcgcgt cgtaaagcac cacgtctgcc tgctggattt cctgcagccc tttgagcgtc
    21601 agcagccccg catcgccggg acccgcgccg accagcgaga cggagccgcc ttgatcattt
    21661 tgacgacttt gttccaattg gcctgccaat tcccgttcgg caagggtgtt ttgccggttt
    21721 ttggcgaggg tggcgaaacg tccgttaaac tgcttttccc aaaagcggcg gcgttcggta
    21781 acggatttca gtttgccctt gacggcatcg cgccaccttc ctgaaatttc cgccatatcg
    21841 cccaaagacg gcggcagcag ggcttccagc ctttcacgca gcagtcgggc gaggacgggc
    21901 gcgctgccgg agctggaaac ggcaatctga accgggttgc ggtcgataac cgacgggaag
    21961 atgaagctgc aatggtcgcg gtcgtccacc acgttgaccg gcttttggca gctttcggca
    22021 agatggaaaa cgcgccggtt gagggcttgg tcgctgcttg ccgcaatgat gaggaaaacc
    22081 gtgcggatgt gttcggcacg aaattcttcg gcaagccaca ggattttgtt ttccgccgcc
    22141 aacgcggaga gttcggcatt caggtgtttt gcggcaaccc tgacctctgc gcccgccttc
    22201 agcagcaggc tgattttgcg tgcggcgacc gcgccgccgc ctacgaccaa tacggggcgg
    22261 ccggcgaggt tggcgaaaat agggaaataa ttcactggct gactcctttg ctgtttgccc
    22321 gcaccttgtt tccgatacgg tgcgtcgcgg catttttgtc ggaatgcggg tcattttaga
    22381 caaaaggatt ttcccggtta aataataaaa aggcatttgt tagaagctga aagctatatg
    22441 agggcggctg cggatgcggc ggttt
//
LOCUS       DAWKOG010000013        22251 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.013, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000013 DAWKOG010000000
VERSION     DAWKOG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22251
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.013"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(120..1034)
                     /locus_tag="WJK66_000402"
     CDS             complement(120..1034)
                     /locus_tag="WJK66_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ7578166.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEHFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1290..2759
                     /locus_tag="WJK66_000403"
     CDS             1290..2759
                     /locus_tag="WJK66_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HEZ7578167.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVAAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAATVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FTGRVLIPVVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3011..3382
                     /locus_tag="WJK66_000404"
     CDS             3011..3382
                     /locus_tag="WJK66_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578168.1"
                     /translation="MNKTLSILPVAILLGGCAAGGGNTFGSLDGGTGMGGSIVKMAVE
                     SQCRAELNKRSEWRLTALAMSAEKQAEWENKICACVAQEAPNQLTGNDVMQMLDPSTR
                     NQALAALTAKTVSACFKHLYR"
     gene            3455..4864
                     /locus_tag="WJK66_000405"
     CDS             3455..4864
                     /locus_tag="WJK66_000405"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEZ7578169.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLSYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDENIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFKATQSNDEQVSFCVPSGNFGNVCAGHIAKQMGLPIR
                     RLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWAEVAAGKGFDLRFALEKVGGKYGFTSGKSTHPDRLATIRQVYEQDQELIDPH
                     TADGVKVAREVREEGETVVCLETALAAKFDATIREAVGDAAIPRPAALEGLETLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            4911..5705
                     /locus_tag="WJK66_000406"
     CDS             4911..5705
                     /locus_tag="WJK66_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578170.1"
                     /translation="MTLPMQETRFSILLDELAAKQEAAIAPHLLADGSKVWIRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRAAGIAVPELLAVRK
                     NALMFGNLEGIPLDTQIRQEAEAGKSDAWLAGLEAIARVHKKRQFLSQAFARNMMSDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            5893..6669
                     /locus_tag="WJK66_000407"
     CDS             5893..6669
                     /locus_tag="WJK66_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ7578171.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            complement(7327..9798)
                     /locus_tag="WJK66_000408"
     CDS             complement(7327..9798)
                     /locus_tag="WJK66_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEZ7578172.1"
                     /translation="MKKTCFHCGLDVPEHLHLTVRYENEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRAGMDTPIAVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHHMPDYPDTQETCEAAVVKLK
                     AGDIVLVKPGETIPVDGTVLEGSSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGNPAVRRIELLGSMTEAQVLAVAQSLEQQSEHPLARAILNCRISGGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVWQLAGKNLTLHILSGDRETAVAETARALGITHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMPSEKMPSEQ"
     gene            complement(9795..10973)
                     /gene="hflX"
                     /locus_tag="WJK66_000409"
     CDS             complement(9795..10973)
                     /gene="hflX"
                     /locus_tag="WJK66_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEZ7578173.1"
                     /translation="MTGRTGRNGSTQAQPERVMLVGVMLDKDGTGSSATRLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKILQCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLANLKKQRALRRKSRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLREIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAESCAAAPNTDETEMP
                     "
     gene            complement(10978..12258)
                     /locus_tag="WJK66_000410"
     CDS             complement(10978..12258)
                     /locus_tag="WJK66_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEZ7578174.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHASYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGSGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
     gene            complement(12411..13130)
                     /locus_tag="WJK66_000411"
     CDS             complement(12411..13130)
                     /locus_tag="WJK66_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEZ7578175.1"
                     /translation="MDTTRIVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAMLAS
                     GIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAKGM
                     EFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGRRAAAP
                     YDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPIKL
                     GVLYYGDESTQYEPAPIRVDK"
     gene            complement(13201..13878)
                     /gene="radC"
                     /locus_tag="WJK66_000412"
     CDS             complement(13201..13878)
                     /gene="radC"
                     /locus_tag="WJK66_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEZ7578176.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTSRSVCSF
                     RQLGLMP"
     gene            complement(14001..15350)
                     /gene="gshA"
                     /locus_tag="WJK66_000413"
     CDS             complement(14001..15350)
                     /gene="gshA"
                     /locus_tag="WJK66_000413"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEZ7578177.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWSVHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNIYALGEILRSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     TAEFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVECVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            15610..17019
                     /gene="leuC"
                     /locus_tag="WJK66_000414"
     CDS             15610..17019
                     /gene="leuC"
                     /locus_tag="WJK66_000414"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEZ7578178.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAIEFGGEAIRSLSMESR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLEAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLTPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            17116..17370
                     /locus_tag="WJK66_000415"
     CDS             17116..17370
                     /locus_tag="WJK66_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578179.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            17432..18073
                     /gene="leuD"
                     /locus_tag="WJK66_000416"
     CDS             17432..18073
                     /gene="leuD"
                     /locus_tag="WJK66_000416"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEZ7578180.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYQLSIDLAEQTLT
                     TPGGETFTFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            18243..19514
                     /locus_tag="WJK66_000417"
     CDS             18243..19514
                     /locus_tag="WJK66_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ7578181.1"
                     /translation="MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFD
                     VARILKAKKPKGFILENVEGLVTHDRKDPTQKIGRTLTVILETLETLGYYVSWKVLNA
                     KDFGIPQNRKRIYLTGSLKSKPDLSFETTLSPKLKNILESGLPTESSPFIKKLLKKFP
                     PSELYGKSVKDKRGGKNNIHSWDIELKGTVTEEEKQLLNMLLKERRKKKWASEIGIDW
                     MDGMPLTKAQISTFYKHPNLQNILDSLTDKGYLVLEHPKQKIGGRRIKDESLPKGYNI
                     VSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSF
                     DIPKKDKYDLLGNTVAVPVIKAVSERLLHTL"
     gene            19530..20261
                     /locus_tag="WJK66_000418"
     CDS             19530..20261
                     /locus_tag="WJK66_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ7578182.1"
                     /translation="MVKLTAQQIFDKLLNEEKILSVNGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGGWLRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNAGPGFDIA
                     DFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWAINLQ
                     VKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKKKFEE
                     AYKKHYNRSISIPRWHEIAHKYKKK"
     gene            20295..21365
                     /gene="leuB"
                     /locus_tag="WJK66_000419"
     CDS             20295..21365
                     /gene="leuB"
                     /locus_tag="WJK66_000419"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEZ7578183.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDISYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTSD
                     IYEEGTKLVSCSEMGDAILAAL"
     gene            21528..22091
                     /locus_tag="WJK66_000420"
     CDS             21528..22091
                     /locus_tag="WJK66_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HEZ7578184.1"
                     /translation="MKKIIFAALAAAAVGTASAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQHFTDHLKSADIFDAAQYPDIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMAKTEVCGGDFSTTIDRT
                     KWGVDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         5363 a         5856 c         5781 g         5251 t
ORIGIN      
        1 ggcatttcca aagggcattt taagccgtat ttgaaagaat gcggatggcg ttttcagtgt
       61 tttcagcgtg ataaaattca aatttctatt ttaaaacaat cagcaaagca ggatttattt
      121 cagttgtccg agacaacggg cggctgcacc gcctgcactc cccatacttt ttcaatgcac
      181 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
      241 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt tgccgccgct
      301 tcggctttgt ttcccggcac cattgccaca cctataccgc ctgcaaccaa atcgataatg
      361 gtttgggggt agtcgcacaa gatctgtttt ttgggtgaga gccggttgct gcgccaaaac
      421 tggtgcaggt gcttcctgct tccggacacg cccgacattt ctatccatac gcattcttgc
      481 aggctctgcg gaagggagcg ggtcagatgg ggatattggc tttgcgggca aatcagcgcg
      541 taggtcaggt tttgcaggaa tacgctgcgg atgccgcgtt gggcggcgtt gccgagtata
      601 aagccgccgt gcagggtttt gtgttggatg cgcgagagga tttctccgct cattccgtat
      661 tggatgtgca ggcgcgtttt gggggctgtt tgaccgatat tgtccgtcag cgcgacgagc
      721 tttgccgaat cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc
      781 agcgttttgg caaaatgctc cagcttgtgt ttgtattgca gcagggattc cgcttcgggc
      841 aacagtattt cgcccgcccg cgtcaatacc atgcctttcc ccgtgcgcct gaacagcggc
      901 gtgccgacat attcttcaag ggctttaatt tgggcagaaa cggcaggctg ggaaaggaaa
      961 agtcgtttgg cggcttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat
     1021 tgtacggcat ccatatatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat
     1081 cttttaggtg gattgttgct gaaaattaac tttttaatca agtggtttgt aaattgtatc
     1141 agttttccgg atgatggtta tcaaaaaaaa agattggttt tgttgcctgt ttgggcttta
     1201 aatggggttg cggcttccga acgcagcccg tatcaaaaag aaaagtcatg cgcccctttt
     1261 acgaggcgcg atatataagg aggaaggtta tggaaaaaca taatgggact tatcgggatt
     1321 tgcaccgtcc agcttcggaa tttgcgacgc gggacgaata tttggaacat gaattgcaga
     1381 ttatgcaacc aaaacgctgg cggcccaacc tgccctttcg cgattaccgc ttcgagtggg
     1441 aggatttgat tcctgcgatg gcgggaacga ttggaaaagt ggtgatggtg ggggcggtgg
     1501 cggcggcgtt tgccgcacct ttggggctgc ctgacagctt tgtactggaa aatgtgcgct
     1561 atgagctttt aatcgccgcc gcgtttatct tattggtatc gggctttttt ctgcccggcg
     1621 ccaacctgcc cggtacgcac gggccgctga ttccgatgat tcccatcgtt gtgtcggcag
     1681 gcgggcatcc tttggcgttc ggcatttcga ttgcggtttt aggtctgctg atggctttat
     1741 ttcgcggcgg cagtattatg gcgaagctga caagcaacgg cgtatgcggc ggattattac
     1801 tctatttggg ctttatcggc acgacggggc aggtaaaaaa attgttttcg tgggcaggcg
     1861 gttttaatat gccctacatc gcttttaccg tcattattgt aacgattgtg atgtacgctt
     1921 tgttggagca ttggaaaaaa cgctggttag ccgtgccttt gggatgcttg attgccggtg
     1981 tggcggcatt tgcattgggt gcgccgtttg agtttcacac cgcccccggc ctgcctccaa
     2041 tgagtcctgc ttattggtgg ggtgaaaaca gcggctggca tctggggttg ccgacggcag
     2101 aaagtttttt ggttgtcttt ccatttgcgg tattggctgt tgcaatgtgg tcgcccgatt
     2161 ttttaggaca tcaagtgttc caaaaaatca gctatccgga aaaaaccgat aaggtattga
     2221 tgaatataga cgacaccatg acaagttgtt ctgtccgtca agcagtgggt tctattttag
     2281 ggggtgcaaa ttttacctct tcttggggaa cttatatcgt accggcatcg attgccaaac
     2341 gccccattcc gggcggtgcg gttttaacgg cggttttatg tattatcgct gggttgtggg
     2401 gctatccgat ggacttggcg atttggcagc cggtattgag cgtagccttg gtcgtaggcg
     2461 tatacttacc gcttttggaa gcgggcatgg aaatgacgcg caaaggcaaa accacccaat
     2521 ccgccgccac cgtggtgttc tcttccgcct tggtcaatcc ggttttcggc tgggcgttga
     2581 cgatgctgtt ggataatttg ggcttaatcg gctgcaaaga gcgcagcgcg caattaggtt
     2641 tcaccggacg cgtattgata cccgtagtag gtttcttgat cttgtgtgtg gcgatgggtg
     2701 cggtcgggat gctgcccggt atcccgccgt ttttggagca cttcaaatct ttgggctagg
     2761 ctgaaatcgg aaatgccgcc tgaaccgctt tcagacggca tttttgcaaa caggcaaaat
     2821 gacggcggcg ggatttttta ttttcccgat tgaagtataa tgttgccggg cttcaaccgg
     2881 atattcaaaa cggtttgttc caacactcgg aacggcgcat aaaacgccgc ccttcgcgtt
     2941 atcccgaacg gggcggctaa tcagattcta tcgccataaa aggcggggtt tcaaccgaaa
     3001 aggaattgag atgaataaaa ccttgtctat tttgccggtg gcaatcttac tcggcggctg
     3061 cgccgccggg ggcggtaaca cattcggcag cttagacggc ggcacaggta tgggcggcag
     3121 catcgtcaaa atggcggtag aaagccaatg ccgtgcggaa ttgaacaaac gcagcgaatg
     3181 gcgtttgacc gcgctggcga tgagtgccga aaaacaggcg gaatgggaaa acaagatttg
     3241 cgcttgcgtc gcccaagaag cacccaacca gctgaccggc aacgatgtga tgcagatgct
     3301 ggatccgtcc acgcgcaatc aggcacttgc cgccctgacc gccaaaacgg tttccgcctg
     3361 cttcaaacac ctgtaccgct aaccttcttc agacggcatg aggcagatgc cgtctgaagc
     3421 cgttttcctg caatttcgac agacgagaaa aatcatgaaa tacatcagca cgcgcggcga
     3481 aaccgcacac aaaccgttca gcgaagtttt attgatgggg cttgcccccg acggcggcct
     3541 gatgctgccg gaacattatc cgcaaatcgg gcgcgaaacc ttggacaaat ggcgcggttt
     3601 gtcttatccc gaattggcgt ttgaaatcat gcgcctgttt gttacggata ttccggaaga
     3661 cgatttgcgc gacattctga accgtactta cacggaagcg gcgttcggta ctaaggaaat
     3721 cacccccgtc cgcacgcttt cagacggcat caaaatccaa gccttatcca acggccccac
     3781 tttggcattc aaagacatgg cgatgcagtt tttgggcaat gcgtttgaat atgttttaaa
     3841 caaagaaggc aaaaaactca atatcttggg cgcaaccagc ggcgatacgg gttcggctgc
     3901 ggaatatgcc ttgcgcggca aaaaaggcgt gaacgtattt atgttgtcgc ccgacggtaa
     3961 aatgagcgcg ttccaacgcg cgcaaatgta cagcctgcaa gacgagaata tccacaatat
     4021 cgccgtgaag ggaatgtttg acgactgtca ggacattgtg aaggcggtgc aaaacgatgc
     4081 cgcgttcaag gaaaaatacc atatcggtac ggtcaattcg atcaactggg gacgcatcgt
     4141 cgcgcaagtg gtttattact ttgcaggcta tttcaaagcg acgcaaagca atgacgagca
     4201 agtcagcttc tgcgtgccga gcggcaactt cggcaacgtt tgcgcgggac acatcgccaa
     4261 acagatgggt ttgcctatcc gtcgcctgat tgtcgcgacc aatgaaaacg atgtgttgga
     4321 cgagtttttc aaaaccggtg cataccgccc gcgcaacagc gcgcatactt atgttacctc
     4381 cagcccgtcg atggacattt ccaaagcgtc caacttcgag cgtttcgtgt tcgacctgat
     4441 ggatcgcgat cctcaggaaa tcaatacgct gtgggcggaa gtagcggcgg gcaaaggctt
     4501 tgacctgcgg tttgctttag aaaaagtcgg tggcaaatac ggttttacct ccggcaaatc
     4561 cacccacccc gaccgcctcg ccaccatcag acaggtttat gagcaagacc aagaactcat
     4621 cgacccgcac actgccgacg gcgtaaaagt cgcccgcgaa gtgcgcgaag aaggggaaac
     4681 ggttgtgtgt ttggaaaccg cgttggcagc gaaattcgat gcgaccatac gcgaagccgt
     4741 cggcgatgcc gccattccgc gccccgccgc gctggaaggt ttggaaacat tgccgcagcg
     4801 cgtgcaaacc gtgccgaaca gtgcggatgc ggtaaaaggc atcatcgaac aaacccttgc
     4861 ctgatgttcc gtatgggcgg cattccgtcc gaacttaaga ttgaaagaaa atgaccttac
     4921 ctatgcagga aacccgtttt tccatactgc tggacgaatt ggcggcaaaa caggaggcag
     4981 ccatcgcccc ccacctgctt gccgacggga gcaaggtatg gatacgcaaa gcaggcaggc
     5041 acaatgcacg atggcgttac gcgctgctcg gtatggttgc ccgatatttg aaactggggg
     5101 tgttgaagcc ggtccccagc ctcggcggcg agcctgccat tgcaaccgaa tcaaaacgcc
     5161 tgtacgaatt gcgcgcggcg gggatagccg ttcccgaatt gctcgccgtc cggaaaaatg
     5221 cccttatgtt cggcaatttg gaaggcatcc cgctcgatac gcaaatccgc caagaagccg
     5281 aggctggaaa gtcggacgct tggcttgccg gtttggaagc cattgcacgc gtgcataaaa
     5341 aacggcagtt cctcagtcag gcgtttgcgc gaaatatgat gtcggacgga aaaaacatca
     5401 gctttttgga ttttgaagac gacccttccg aagtcctgac catagcgcaa tgccaagccc
     5461 gcgattggct ctgctacatc cattcgaccg cgctgatatt gaaaaacggc ggactgctgg
     5521 aagcggctgc ggaaaaatgg ggcggcgtat tgtcagatca gcctgccgaa atacaaaagc
     5581 tgattgccgc caccgtcaaa ccgatcctcc cgatacgcag gctggaacac ccccgctggg
     5641 gacgggacgc gctcaggctg gcagcctcga tttccctgat ttcccttgcc gatatgccgc
     5701 cgtaagccgc cgcccgcccc acgatgccgt ctgaagccct tcagacggca tataaccaac
     5761 gcgcatcaag cattgtcgca accatccgtt tcgtttagaa ttttggcttt accccgaatc
     5821 gggcagacat tttcactctg ccgcacatcc cgaacaagca ctatccattt cttttactga
     5881 caggaaaccc aaatggcagc attcaatacc caaaaagtat tgtccgtaca ccactggaca
     5941 gacgcatatt ttacctttac ctgcacccgc gacgaatcgt tgcgctttga aaacggacag
     6001 ttcgttatgg tcggattgat ggtggacggc aagccgctga tgcgtgcata cagcgtcgcc
     6061 tccgccaact gggaagaaca cctcgaattt ttcagcatta aagtccaaga cggcccgctg
     6121 accagccgcc tgcaacacct caaagtcggc gacgacgtgt taatcagcaa aaaaccgacc
     6181 ggaactctag ttgccggcga cctgaatcca ggcaaacacc tttacttgtt gagcaccggt
     6241 accggcatcg cccctttctt gagcatcacc aaagaccccg agatttacga gcaatttgaa
     6301 aaaatcatcc tcgtacacgg cgtgcgctac aaaaaagatt tggcgtacta cgaccgcttt
     6361 accaaagaat tgcccgaaca cgaatacctc ggcgacttgg ttaaagaaaa actgatttac
     6421 tacccgattg tttcccgaga agaattcgaa caccacggcc gcctaaccga cctgatggtg
     6481 agcggcaaac tgtttgaaga catcggcctg cccaaaatca atcctcaaga cgaccgcgcg
     6541 atgctgtgcg gcagccccgc gatgctgaaa gacacctgca aagttttgga cgatttcgga
     6601 ctaaccgttt ctccgaaaac aggcgtgcgc ggcgattacc tgattgagcg cgcgtttgtg
     6661 gatcaataag ctgcttgaaa atgccgtctg aacggcatca gcgataaaaa aacccgaatc
     6721 ctgccaatca ggattcgggg tttttatagt gggtaaaaat tagaaatgca catttccgtc
     6781 attccaacac aggtgggaat ccggacttac ctgtttcatt atcgtttgaa aataaaagag
     6841 aaaccaaaca tatagattcc cgcttgcgcg ggaatgacga cggtgttttg ccgtagccaa
     6901 agcagaaaaa catccctatt gtcatttcat catgatatga caataggatg ttaaaaatta
     6961 attatcatta tatgattgat atataagaaa aactgtccca ataagaaatg ttatgcagaa
     7021 aataaaatga aaaggatggt tttcattgaa aatggttgtt ctgccaccgt catacacaga
     7081 accggtattt ggaaaaaaaa taatccatat tgacatcatt attccattta tcgcataaat
     7141 ccacttttta attttttttc ctcattaata taatctctta tttaataaaa tcaaatacct
     7201 agcaattcga tctcaaattt taaacgccaa cgtgtttgag cggtaacaat gcatacttta
     7261 agtttgtaat ccttcatcgt ctcatttttg ttttaatcga ctatacgtct aaagcaacgc
     7321 cgtccgtcat tgttcggacg gcattttttc agacggcatt ttcccccgtt tgtgaaggcg
     7381 cagggcgttg cccaaaaccg ccagcgaact gaagctcata cccagtgcgg ctatccacgg
     7441 ttggacatag cccaaaacgg caagcggtac ggcaatgata ttgtacgcgc ccgcccatat
     7501 caggttttgc cggataatat ggcgggtgcg ccgcgcctga tcgagcaggt gggcgacggt
     7561 acgcaaatct tcgttcaata acacaatgtc cgcgccgtcc ctcgcaatat ccgtcccgcc
     7621 cgctgcggcg gcggatacgt ctgcctgcgc caaaacgggc gcgtcgttga tgccgtcgcc
     7681 tatcatcagc acttttttcc cttctttttg caaggctttg acgtattcca gtttgtcctc
     7741 gggcatggct tgggcgcggt agtgcgtgat acccagggcg cgggcggttt cggcaacggc
     7801 ggtttcgcgg tcgccgctga ggatgtgcag ggtcaggttt ttgcctgcca actgccacac
     7861 cgcctccgcc gcgctgtctt tcaaggggtc ggtcaggtag aacacggctt ggaaaccgct
     7921 ttgactgccg aggtaaaccg cgctgccgcc gccttctgtt tgcggttctt tacctgaaat
     7981 ttcggcgaca taggatgccc tgcccaatgc ccaaacctgt gtttcgccgt tgacggtcag
     8041 ttgcgcgccc acgccttcgc cgatgcggtt gaggcgttgt ttaatagcaa tgtcggggac
     8101 gctgccgcct gaaatgcggc agttgaggat ggcgcgcgcg agcgggtgtt cggactgctg
     8161 ttccaaactt tgcgccactg ccagcacttg ggcttcggtc atgctgccga gcagttcaat
     8221 ccggcggacg gcgggattgc cttgggtcag cgtgccggtt ttgtcgaaaa tgatgtcggt
     8281 ggtttgggcg agggtttcga ttgcctgctt tccgccgatt aaaatacctt cgcgcgccag
     8341 cgtaccggta gaagctgcca gcgcggtcgg cgtggcaagc gataaggcgc aggggcaggt
     8401 aatgaccagc agggcgacgg taatccacaa tgcggtgtgc gcatcggcgt acaacgtcca
     8461 gccgatgaag accgggacgg caagcagtag ttcgccgaat atgaaagacg aggcgtattg
     8521 ttccgccaac tcggcggtgc gcggtttttg cgccaaggcg cggtcgagca agcggacgat
     8581 gtgcgacagt cgcgtgccgc cgccggtgcg gtcggtgcgt ataatcaggg ggctttgcgt
     8641 gttgagtgtg ccggcggtta ctttttcaga cggcattttg gcgacgggca ggctctcgcc
     8701 ggtcagcata gattcgttga cggcactgct tccttccagc accgtgccgt caacggggat
     8761 ggtttcgccc ggtttgacca gcacgatatc gcccgccttc aatttgacga cagctgcctc
     8821 gcaggtttcc tgcgtatcgg ggtaatcggg catatgatgg caaaacgcag gaatcagctt
     8881 caccagcctt tcggcagcat cgcctgcctt acggcgggca atgtgttcca taaagcgtcc
     8941 gcccagcagg aaaaacagca gcatggctat ggattcgaaa tacatcccct gccccgcatt
     9001 ggtcgcaagg ctgtaaacgc cggcgataaa ggtcatgatg atggcgacgg caatcggcgt
     9061 atccatgccg gcgcggcggt ttttcaagtc gcgcagcgcg ccttgataaa acgggacggc
     9121 gcaatagaat acgacgggca gcaccattaa aaagccgccc caatggagga tttgcaggaa
     9181 atcgggttcg atgtcgccgc cgtaaaggta ggtcggcagc gcgaacatca tcgtctgcat
     9241 catccccagc ccggcaacgg cgaggcggac gatgtattgt ttgcgttctt tttggttggc
     9301 ggcttcgatt ttttgcgcgt catagggtgc ggcggtgtag cctatctgcc tgattttcaa
     9361 cagaatgtcg gaaaggcgga ttttgccgtc gtcccagacg acgcggcagc ggtgcgtgct
     9421 gtaattgagg tcgatgcgga cgatgccgtc tgtacgcaaa agctgctgtt cgatcagcca
     9481 gacgcaggcg gcgcaggtga tgccgccgag cattaaaacc gcctcgcgcg tgccgccgtg
     9541 ggtttccaca aagtcggact ggacttcggg caggtcgtac aggcggattt ggtcgaggat
     9601 ttcttggggc ggcagctcgg ttttttgcgc gtcggcggtg cgttgtttgt aataactgcc
     9661 caagcccgcg tcaataatgc tttgtgcgac cgcctgacag ccggcgcagc aggtttcgcg
     9721 gtcttcgttt tcgtaacgga cagtcaggtg gaggtgttcg ggaacatcca gcccgcagtg
     9781 gaaacaggtt tttttcatgg catttcggtt tcgtctgtgt ttggtgcggc ggcacaagac
     9841 tcggcaatgg cttcgcgcag tgcgtctata ccggtatttt cagcaacgga aatgcggacg
     9901 gcggcaattt ttcccgcagc gtcgcgccat atgcccgtgt tttgttcttc agacggcagc
     9961 aggtcggttt tgttgtacac cttgatgcac ggaatatcgc cggcatggat ttctcgcagt
    10021 acgttttcca cgtcttcaat ctgctgtccg ctgttcggag cggcggcatc gacgacgtgc
    10081 agcagcacat cggcttgcgc ggtttcttcc agcgtggcgg aaaaggcgga aatcagtttg
    10141 tgcggcagat cgctgacgaa tccgacggta tcggtcagga tgatgctgca ttcgggactg
    10201 atgtacagcc gccgcgccgt ggtgtcgagt gtggcgaaaa gctggtcttt cgcatatatg
    10261 cccgacttgg tcagccggtt gaacaggctg gatttgccga cattggtata gccgaccagc
    10321 gcaaacgttt tgattgtgcc cgattcgcgg gacttgcggc gcagggcgcg ctgttttttg
    10381 aggttggcaa gctgtttttt caaggcgttg attcgatggg cgatcaatcg gcggtcggtt
    10441 tccagtttgg tttcgcccgg ccctttcatg ccgataccgc cgcgctggct ctgcagatgg
    10501 ccgtaaccgc gtatcaagcg tcccgccaaa tggctcaatt gcgccaactc gacttgcagc
    10561 ctgccttcct gcgtgcgggc gcggcgggcg aaaatcgcca aaatcaaccc taccctgtcc
    10621 aatacgcggc attggaggat tttttccaaa ttgcgttcct gcgtgggcgt aagttcgtgg
    10681 ttgaatacga ccaaatcgat gccgtctgcg gcaactgctt ccgacagctc cgccgccttg
    10741 cccgtgccga caaacagcgc ggtgtgcgga cggtcgcgtt tggcagtctc cacgcgcacg
    10801 gaatcgccgc ccgccgcttt gaccagctcg acagcttccg ccaatgccgt ctgaaaaccg
    10861 ttcagacggg tggcactgct gcccgtgcca tctttgtcca acattacgcc caccagcatg
    10921 acgcgttcgg gttgcgcttg ggtactgccg ttgcggcctg ttctgcctgt catgtattca
    10981 atcctgtaaa taaagggtta cgcctcgttc cgtttctcct tccggtgcaa gcgtgatgcg
    11041 gctgcccgcc attccgtcaa cgctgaagac gatgccgccc gcctcgtgcc ttcctatgga
    11101 caggcggctg ccgggtcgct gccggtaggt ataacgggta cacgcttcca aacaccagcg
    11161 tccgcgccag tggatgaaat atttttcgcc ttttctaacc gcaagtgtct cgccgccgtt
    11221 tacctcaaaa taagggtaat cgggttttcg ggttctaaac tgccatttcg cctgcgctcg
    11281 ccgtccgttg cggacatagt cgaataccgc cgtataaagc gtgccgtatt ccaaaggctt
    11341 gagcggaaaa agcgcgaatt ggtacgcggt cagcctgccg ttggggtcgt tgccggcggt
    11401 taaaaccctg acggggcgga tttcgttttt accctgatac agcttgaaac ttttcatcgt
    11461 aattttgcct gccgcctcgg aaaaatcaat gctggcagga ttgcccgtga tttcatattc
    11521 cggcacgggg tctggacgct cgccgtggaa ataaggcagt gcgccgctgc cgacgggata
    11581 ggctgtatag agcagctcct gtgcgggcat ggcttcgtcg gtgtacacga ccgcaccgtt
    11641 atggcaggcg ttgcggtaat atttccgtcc tgcttccggc tgatttctgc cttgggcgca
    11701 atgccgctca aacctgccgt tgccctgatt gaatacgaga acggttttac cgttttcgcg
    11761 cacaaatgcc gctcctgcct catccgtatg gcggtcaagt agggaaaggc ggtggtagat
    11821 tgcgctcatt aatccgtcca cttggcgttg ctgcgtgcgg atgtcgctgt cggacgattc
    11881 ggcggcttct tcttccgtgc tgatgttttc atgcacgccg ttgtagagat acccggcaag
    11941 gcgtgtgcgt tcggtcagct tttgtgcggt gtagtgcgga ttgtcgggat ggtgttcgcc
    12001 gtgtccgtct tcgggattga gcgtgaggta gcttgcgtgc ctgcgggcgg agttttccaa
    12061 aaccggcgcg tgtgccagct tgtgcaaacc gatttgggct cggatgcggt tgaggtagtg
    12121 cagcgcatcg ccgccgtccc tgatttgcgg tgcggacgga tagacaagtt cgcccgcggg
    12181 cagggattgg ttttgggtat ggtagaaaac gcctgccgcc gaacccaaca ggagcagccg
    12241 aataaaaagg gatttcataa tgccgtcctc ccgacgcggc aacggggtgt tttatcaaaa
    12301 cggcaaactt gccaatgcgg tttccgattg cagattgcca aaccggcaaa cctttccccg
    12361 tgccgatgcc gaacccgatg ccgtctgaac cttcagacgg catcgggtgt ttatttgtcc
    12421 acccggatgg gggcaggttc gtattgtgtc gattcgtcgc cgtaatacag cacgccgagt
    12481 ttgatgggaa tgcgtccctg cgatttgcgg tgggcattgg aatcgcgcaa ggaataggcg
    12541 caaccgcaat attcctgctg gtagaagttt tcacgtttgc tgatttcaat catgcgcgcg
    12601 ctgccgccgc ctttgcgcca gttgaaatcc caatacacca catcatcgta aggcgcggcg
    12661 gcacggcggc cgcagtcgtt gatttgcgcc atatttttcc agcgtgaaat gcccagcgaa
    12721 ctggtaaaga cggggaaccc gtgttcatgc gcgtattgcg ccgccttttc aaaacgcata
    12781 tcgaaacaca tggtgcagcg gatgccgcgt tccggctcaa actccatgcc tttggctttg
    12841 gcaaaccatt ctttgcggtc gttttcgtag tcgtcgtctt tatcgatgaa aggaatgccg
    12901 aacttttccg caaagcgcac gttttcctct tttcggagca tatactcttt gtgcggatgg
    12961 atattgggat tgtaaaaata aatggtgtag tcgatgccgg aggcaagcat cgcctccatc
    13021 acttcgccgc tgcacggggc gcaacaggaa tgcagcaaga cttttttatg tccgccgggc
    13081 gggacgagta tggggcggtc gatgtcggta acgatgcgtg ttgtgtccat agtttcaaat
    13141 agatgttctg tttggtgcaa acggctattt tatcagagcc gccgcatgtg aaacagagtg
    13201 tcagggcatc aacccgagct gcctgaacga gcagacgctc cgggaggtta cgataaaatg
    13261 gtcgagcagc gacacatcga ccagcgacat ggcctgtgcc agccgccttg tgaacatgat
    13321 gtcttcctgc gaaggttcgg gcgagccgcc cggatggttg tgtgcaataa tcaggctgtc
    13381 ggcatattcg tccaatgcca gtttgacgat ttcgcggatg taaatcgtgt tttccgcaac
    13441 cgtaccgcgc gacagctctc tgaccgcaat cagttggttt tggcggttca gcagcagcgc
    13501 gacgctgact tcgacttttt cctgccccaa atgaaagcgt aaataatcgg caacggtatc
    13561 cggatcggac aggacgatgc tctcctgcaa ttcttccgcc aatatccgcc gcccgatttc
    13621 cctgactacg gcaaactgtg tgaaacttgc cgtccccatc cctttgtatg ccgacagttt
    13681 gccgacctcc gcgctcatca gcctccccaa actgccgaac tcctgcagca aataacgcgc
    13741 caaatcgacc gcactcattc cgcgcgtgcc gacgcgcagc aggattgcca aaagttcggc
    13801 atcactcaaa gccgccgccc cgcgttccaa cagcttttcc ctgggtcttt cgccttccgg
    13861 ccattgcttg atgctcatgt tttccctttt ttaaatagtc tgagatgccg tctgaaacaa
    13921 atgttcccac acgccaaata tccgaaatgc cgtctgaacc ggatttccgc ttcagacggc
    13981 atctgtattt caaaacagtc ttacgccgtt tcttccaact cgattgaagc cgccagaagc
    14041 gacaggcgtg ccattacgcc gtaaacgtag aggcggttgt gttcgttgtc cacgtcgcag
    14101 tctttttcag agcgcggcat acccagcgag tcccattgtt cgaatacgcg cttgcaggct
    14161 tcgggcgctt cttgggagtt gtcgccgttg ccggtgggga tgctgttgga cagcggaaca
    14221 aacaccatac cgccggcgtt gaggttttcg tccgcaccgc gcccttcgtg tacgcggaaa
    14281 aagccgccga tgacgaagcg gtccatcata tacacgacgg gttcgcacac cgcgccgttt
    14341 aaggtttcat aagtataaat cccttcttgg acaatcactt cgctgacttc caaaccttct
    14401 ttgactttcg ccattttatt gcggttttta cggttcaatc cgcgcacttc gtcggcggat
    14461 ttgacgctca tcacgcccat gccgtaagtg ccggcatcgg ctttgacgat gacgaaaggt
    14521 ttgtcggtaa tgcccgattc gtcgtatttg gcttgaattt tcgccagcac acattctacc
    14581 gcttccgcca acgcgtcttc gccttcacgc ccttggaagt ccaaaccgcc gattttttca
    14641 aaataagggt taatttgcca ttcgtcgatg ccgattaact tggcaaattc ggcggtaact
    14701 tggttgtacg cgccgaaatg atttgttttg cggcgcgtcg tccaaccgcc gtgcaacggc
    14761 ggcaaaacgg tttggctgat gcctttgagg atgtcgggaa tgccggcaga caaatcgttg
    14821 ttcaacaaaa ccacgcaagg cgaaaagccg tctgcaagat ggacgcggtc gcgggtacgc
    14881 aataaaggtt ccaacaggat tttgtcgccc aatgcagttt caaattcggt cggttcggtt
    14941 acttccggat tcaggctgcc caagcgcact tcataccctg ccgaacgcaa aatctcgccg
    15001 agggcgtaaa tgttttgcag gtaaaacgta ttgcgcgtgt ggttttcggg aataatcaat
    15061 acggatttcg ccgtttcgca ggcgcgttgc accgcatctt gcgcggcaac cgccgccagc
    15121 gggataaagt tgggattcag attattgaag ccgccgggga acaaattcat atcgatagac
    15181 gaaattttgt aaccggcatt gcgtatatcg accgaaccgt aaaacggcgg gcggtgtacg
    15241 ctccactgcg tgcggaacca cgcctcgatt ttggcgtgat tggacaggat ttttgcctca
    15301 aacgcctgaa gttgcgccga atgttcgggc gacatcaccg gtaatttcat ctttcgtctg
    15361 cctctgtgtg ttttttgaca agatgcagat gataatgcct ttgaaagaat gacgacaagt
    15421 attctttcgt atcgaatcat gctttagata taactgttta ctgaaatgga caatacgtct
    15481 aattttttcg ttaaaattgt ttgcaaaata acaccggctc aaaaattaga caaaatttgt
    15541 tgcgcggtat aaagaatacg tctaaaatcc gcccaatccg cattaccatt tatccaaaag
    15601 aacaacatca tgacagcaca aaccctctac gacaaacttt ggaacagcca cgtcgtccgc
    15661 gaagaagaag acggcaccgt cctgctctac atcgaccgcc atttggtgca cgaagttacc
    15721 agccctcagg catttgaagg cttgaaaatg gcggggcgca agctgtggcg catcgacagt
    15781 gtcgtctcca ccgccgacca caacaccccg accggcgatt gggacaaagg catccaagac
    15841 ccgatttcca agctgcaagt cgatactttg gacaaaaaca ttaaagagtt tggcgcactc
    15901 gcctattttc cgtttatgga caaaggtcag ggcatcgtac acgttatggg ccccgaacaa
    15961 ggcgcgaccc tgcccggtat gaccgtcgtc tgcggcgact cgcacacttc cacacacggc
    16021 gcattcggcg cactggcgca cggcatcggc acttccgaag tcgagcacac gatggcgacc
    16081 cagtgcatta ccgcgaaaaa atccaaatcc atgctgattg ccgttgacgg caaattaaaa
    16141 gcgggcgtta ccgccaaaga cgtggcactt tacatcatcg ggcaaatcgg cacggcaggc
    16201 ggtacaggct acgccatcga gtttggcggc gaagccatcc gcagcctttc tatggaaagc
    16261 cgcatgactt tatgcaatat ggcgatcgaa gccggtgcgc gctcaggcat ggttgccgtc
    16321 gaccaaacca ccatcgacta tgtaaaagat aaacccttcg cacccgaagg cgaagcgtgg
    16381 gacaaagccg tcgagtactg gcgcacgctg gtgtctgacg aaggtgcggt attcgacaaa
    16441 gaataccgtt tcaacgccga agacatcgaa ccgcaagtca cttggggtac ctcgcctgaa
    16501 atggttttag acatcagcag caaagtgccg aatcctgccg aagaaaccga tccggtcaaa
    16561 cgcagcggta tggaacgcgc ccttgaatac atgggcttgg aagccggtac gccattaaac
    16621 gaaatccccg tcgacatcgt attcatcggc tcttgcacca acagccgcat cgaagacttg
    16681 cgcgaagccg ccgccatcgc caaagaccgc aaaaaagccg ccaacgtaca gcgcgtgtta
    16741 atcgtccccg gctccggttt ggttaaagaa caagccgaaa aagaaggctt ggacaaaatt
    16801 ttcatcgaag ccggttttga atggcgcgaa ccgggctgct ctatgtgcct cgccatgaat
    16861 gccgaccgcc tgaccccggg tcaacgttgt gcctctactt ccaaccgtaa ctttgaaggc
    16921 cgtcaaggca acggcggacg tacccacctc gtcagccctg ccatggcagc cgccgccgca
    16981 gtgaccggtc gctttaccga catccgcatg atggcgtaag tattttcagg cggtctttaa
    17041 gaatttgacg cggccgtctg aaatatgtcc cgtaaggcag aaacaccttt aatattcaaa
    17101 ccaaggagtt taaccatgaa caaacttttc attaccgccc tgtccgccct cgccttgtcc
    17161 gcctgcgccg gcacttggga gggcgcgaaa caagacaccg cccgcaacct tgacaaaaca
    17221 caggccgccg ccgaacgcgc cgccgaacaa acaggcaacg ccgtcgaaaa aggctgggac
    17281 aaaaccaaag aagccgtcaa aaaaggcggc aatgccgtcg gacgcggcat ttcccatctc
    17341 ggcggaaaaa tcgaaaacgc caccgaataa ccgattcgcg atgccgtctg aaaccgcttc
    17401 agacggcatc gtcagcacaa gacgcaaacc catgaaagcc tttaccaaaa ttaccgccat
    17461 cgtcgccccg ctcgaccgca gcaacgtcga taccgacgcc atcatcccca aacaattttt
    17521 aaaatccatc aaacgcagcg gcttcggccc caatgccttt gacgaatggc gttacctcga
    17581 ccacggcgaa ccgggcatgg acaacagcaa acgcccgttg aaccctgatt tctcgctgaa
    17641 ccagccgcgc tatcaaggcg cacaaatcct gttgacgcgt aaaaacttcg gttgcggctc
    17701 ttcacgcgaa cacgcccctt gggcattgga cgactacggc ttccgcgccg tcatcgcccc
    17761 cagcttcgcc gacatcttct tcaacaactg ctacaaaaac ggccttttgc ctatcgtttt
    17821 gaccgaagaa caggttgacc ggcttttcaa agaagttgaa gccaacgaag gctatcagct
    17881 ctccatcgac cttgccgaac aaaccctgac cacccctggc ggcgaaacat tcacattcga
    17941 cattaccgaa caccgcaaac actgcctctt aaacggcttg gacgaaatcg gactgaccct
    18001 gcaacatgcc gatgaaattc acgcctttga agaaaaacgc cgccaaagcc agccttggct
    18061 gtttaacggt taaaacctat accgtctgaa ggccgttcag acggcattcc tgataacata
    18121 taattgctgc aactaatgta aacagagggg ctataaattg ctttcatctt tcaagcgggc
    18181 ttagcaacgc tctaaattaa tgcaatacag ctctatttca aataaatttt tagattttta
    18241 atatgcaaca gattaaattt attgacttat tttccgggat gagcggtatc aggaaaggat
    18301 ttgaacaagc ctgtcggaaa cagtcggttg cttgcgaatg tgtttttacc tcagaaatca
    18361 aaccggcagc tttggaagta ttgaagcaaa actaccctga tgaagtgccg tatggagata
    18421 taacgaaaat tgaaacggga gatattcccg attttgacat cttgttggca ggcttccctt
    18481 gccaggcttt ttctttcgcc ggaaaaagat tgggctttga agatacgcgg ggaacgcttt
    18541 tctttgatgt ggcaaggatt ttaaaggcaa aaaaaccaaa aggttttatt ttggaaaatg
    18601 tggaaggatt ggtgacgcac gatagaaaag atccgacaca aaaaatagga cgcaccctga
    18661 ccgttatttt ggaaaccttg gaaacattgg gctactatgt ttcttggaaa gttttaaatg
    18721 caaaagattt cggcattccc caaaacagga agcgcattta tctgacaggc agtttgaaat
    18781 ccaaacccga cttatctttt gaaacaaccc taagtccgaa attaaaaaat attttggaat
    18841 cgggactgcc taccgaaagc agtcctttca tcaaaaaatt gctaaagaaa tttcccccgt
    18901 ccgaactgta cggaaaatca gtaaaggaca aacggggagg gaaaaacaat atccacagtt
    18961 gggatattga attaaaaggc acagtaaccg aagaagagaa gcaattatta aatatgcttc
    19021 taaaagaacg gaggaagaaa aaatgggctt cagaaatcgg catcgattgg atggatggga
    19081 tgcctttgac aaaagcgcaa atttcaactt tctataaaca ccccaatctt caaaatattt
    19141 tggacagcct gacagataaa ggctatttgg ttttggagca tccgaaacaa aaaattggcg
    19201 ggcgaagaat caaagatgaa tccctgccca aaggctacaa tattgtttca ggaaaaaaat
    19261 catttgaaat caataaaata ttagacccaa atgatgttgc gccaacctta gttgcaatgg
    19321 atatggagca ccttttcgtc gttgacaacg gcggtttgcg cacacttacc ggaaaagaag
    19381 gtttacgctt attcggctat ccggacgatt attcgtttga tattcctaaa aaagacaaat
    19441 acgatttatt gggcaatacc gttgccgttc ctgtgattaa ggcggtatct gaaaggcttc
    19501 tgcatacttt ataaaccgat agggataaaa tggtaaaact tactgcacaa caaatatttg
    19561 ataagttgct gaatgaggaa aaaatcttat cggtcaatgg tcaaattaga tttttcttgg
    19621 gcgatgtgga tattatcgtc aaacaaaaag atgtcgtcgg caacatcatt caagaatggc
    19681 tcggcggatg gttgagaaaa agggaaattg aatttgatgt ttcaaccaat acccaaatgc
    19741 cccccgattt ctttttaaat aaaaaagacc gcagtagaga attgttggaa gtaaaggcgt
    19801 tcaaccgaaa tgccggcccg ggttttgata ttgcagattt taaaatgtat tctgatgaaa
    19861 tcattcataa accctatatg ctggatgtag attatttaat attcggttac gatatggacg
    19921 acaacggcaa tgtaaccatc aaggatttat ggcttaaaaa agtatggcaa attacgagaa
    19981 gcatggatgg gtgggcaatt aatcttcaag tcaaaaaagg cgtggtgcat aaaatccgcc
    20041 cgggtgtttg gtacagcata aataaaaaga atatgcctat gtttgaatgc ttggaagatt
    20101 ttgtttccgc aattgaagaa accgtttatc aaaacccggc aacacggcat aacgcatcct
    20161 tatggaaaaa gaaatttgag gaagcctata aaaaacacta caaccgatcg atttccatac
    20221 ctcgttggca cgaaattgca cacaaataca aaaagaaata acccttcaac catcaaccca
    20281 taaggaaact tgaaatgacc aaacatatcg ccatcctccg aggtgacggc atcggtcccg
    20341 aaatcgtcgc cgaaaccgtc cgcgtactcg acaaactcat cgcccaaggc ttggacatca
    20401 gctacgaata cgccccattg ggtggtgaag cctacgacga atacggccat ccttatcccg
    20461 aatttacgca aaacctctgc cgcaaagccg atgccgtcct gcttggtgca gtcggttctc
    20521 ctcaatacga caacctcgac cgtccgttgc gccctgagcg cggcctgttg gcaatccgta
    20581 aggatttgaa cctgtttgcc aatttgcgtc ctgctgtttt atatcccgaa ttagccaacg
    20641 cctccacgct gaagcctgaa atcgttgccg gtttggatat tttgattgtg cgcgaactca
    20701 ccggcgacat ctatttcggc gaaccgcgcg gcatccgcgt tctggaaaac ggcgaacgcg
    20761 aaggctacaa caccatgaaa tacagcgaaa gcgaaatccg ccgtattgcc catgttgcct
    20821 tccaatccgc ccaaaaacgc agcaaaaaag tctgttccgt aggcaaagcc aacgttttgg
    20881 aaaccaccga actgtggcgc gaaatctttg aagaaatcgg caaacaatat cccgatgtcg
    20941 aactctccca tatgtatgtg gacaacgccg ctatgcagct cgtgcgcgcg cccaaacaat
    21001 ttgacgtgat tgccaccggc aacatcttcg gcgatatttt atcggacgag gcctctatgc
    21061 tgaccggctc catcggtatg ctgccttctg cttctttgga cgaaaacggc aaaggcctgt
    21121 acgaaccgtc tcacggctcc gctcccgaca tcgccggaca aaacaaagcc aacccgctgg
    21181 ccaccatcct ctcgctcgca atgttgctgc gttacagcct gaacgacgaa gcgcgcgcgc
    21241 aacaagttga aaacgccgtc caaaaagtgc tgcaacaagg cttgcgtacc agcgacattt
    21301 acgaagaagg cacaaaactc gtttcctgct ccgaaatggg cgatgcgata ctcgccgcct
    21361 tgtaaaaaaa caaagcgcgg atcacaccgc gctttttcat ctgccctttt ccgcccgcac
    21421 cgttcgtcaa ccgcctgcaa acccaccgtt taccggcagg aaatgccata aatccccgcc
    21481 tatgttagga taaacacctt tatcggccca cacaaaggat tatcatcatg aaaaaaatca
    21541 tcttcgccgc actcgcagcg gcagccgttg gcactgcctc cgccgccacc tacaaagtgg
    21601 acgaatatca cgccaacgcc cgtttcgcca tcgaccattt caacaccagc accaacgtcg
    21661 gcggttttta cggtctgacc ggttccgtcg agttcgacca agcaaaacgc gacggtaaaa
    21721 tcgacatcac catccccgtt gccaacctgc aaagcggttc gcaacacttt accgaccacc
    21781 tgaaatcagc cgacatcttc gatgccgccc aatatccgga catccgcttt gtttccacca
    21841 aattcaactt caacggcaaa aaactggttt ccgttgacgg caacctgacc atgcacggca
    21901 aaaccgcccc cgtcaaactc aaagccgaaa aattcaactg ctaccaaagc ccgatggcga
    21961 aaaccgaagt ttgcggcggc gacttcagca ccaccatcga ccgcaccaaa tggggcgtgg
    22021 actacctcgt taacgttggt atgaccaaaa gcgtccgcat cgacatccaa atcgaggcag
    22081 ccaaacaata aatcttgatg cgaatcagca caaatgccgt ctgaacgtcc ttcaaacggc
    22141 atttgtgccg tcttcatcaa accgccccgc caacggtaag ccgatatggc ggagcacaat
    22201 ccgccgaaag atatttcagc ggcaggcagg gcttgccgga aagtccggaa a
//
LOCUS       DAWKOG010000014        21860 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.014, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000014 DAWKOG010000000
VERSION     DAWKOG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21860
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.014"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(27..1226)
                     /locus_tag="WJK66_000421"
     CDS             complement(27..1226)
                     /locus_tag="WJK66_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HEZ7578185.1"
                     /translation="MSTFPNHTMHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDF
                     NSHSLKNGLPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSG
                     VRSLLRLQPEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHN
                     GKEALTAIDTALKLFDKVNIDLMYALPNQTVQTALDDVQTAIATGATHISAYHLTMEP
                     NTPFGHTPPKGLPQDEAALDIEDAVHGALESAGFVHYETSAFAKPAMQCRHNLNYWQF
                     GDYLGIGAGAHGKISYPDRIERTVRRRHPNDYLALMQNRPSEAVERKTVAAEDLPFEF
                     MMNALRLTDGVPTAMLQERTGVPSAKIMAQIETARQKGLLETDPAVFRPTEKGRLFLN
                     DLLQCFL"
     gene            complement(1259..3784)
                     /gene="ligA"
                     /locus_tag="WJK66_000422"
     CDS             complement(1259..3784)
                     /gene="ligA"
                     /locus_tag="WJK66_000422"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEZ7578186.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHIRHLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALERNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGKPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNIKTVSNIPLRLHGENTPELIEVRGEVLMQKADFAALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARQQGGFVAEEHIQELAYF
                     QALGFSLPNGNFGCFKNIDEVLAFYEQMQQKRPELPYEIDGMVVKVNSLAQQRELGFI
                     SRAPRWAIAHKFPAEEALTIVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMQETAVAVSDGIGHRQDDLFAEA
                     PAANQTQSVPLHKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVLHFADLYRLDIPTLQKMKETADKTVAESDQMPSEG
                     SSVGASGKHKKQPVKWAENILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVARSIAHFFAQAEQQAMIDELLAAGVAPQTQAVTIPP
                     ARHAEPQRWIARLPDFKISENKAQALWELAGKNIEGLQNDKALPADWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSILSEAELLTLLG"
     gene            complement(3932..5218)
                     /locus_tag="WJK66_000423"
     CDS             complement(3932..5218)
                     /locus_tag="WJK66_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEZ7578187.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGPVMMPKPQPAVKKTAKPQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPVPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFTEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            complement(5412..5984)
                     /gene="ampD"
                     /locus_tag="WJK66_000424"
     CDS             complement(5412..5984)
                     /gene="ampD"
                     /locus_tag="WJK66_000424"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEZ7578188.1"
                     /translation="MDNPATAPWQDGWLQSVRHIPSPNFGPRESKEEVSLVVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLRVSSHFLIKRDGETVQFVSCDNMAYH
                     AGVSSFRGREKCNAFSIGIELEGCDFEPFTEAQYRSLEALLDAICRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIRGKGFPVDRNAV"
     gene            6068..7063
                     /gene="mltG"
                     /locus_tag="WJK66_000425"
     CDS             6068..7063
                     /gene="mltG"
                     /locus_tag="WJK66_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEZ7578189.1"
                     /translation="MLRKLLKWTAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLRIYQTAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLVEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            7123..7743
                     /locus_tag="WJK66_000426"
     CDS             7123..7743
                     /locus_tag="WJK66_000426"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEZ7578190.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQTREKDRFEQEQAD
                     FFMRVRSVYLNRAAACPERYAIIDSNRSLDEVRNSIEKVLDGHFGC"
     gene            7965..9245
                     /locus_tag="WJK66_000427"
     CDS             7965..9245
                     /locus_tag="WJK66_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HEZ7578191.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKQFYAIEDNGQRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADFEAYAAKLGEWKL"
     gene            9328..9599
                     /locus_tag="WJK66_000428"
                     /pseudo
     CDS             9328..9599
                     /locus_tag="WJK66_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9639..10673
                     /locus_tag="WJK66_000429"
     CDS             9639..10673
                     /locus_tag="WJK66_000429"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEZ7578192.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNAASRAEDAGDEPLLLFSKTGAPTAVGSSRAEVGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPAADTGRTDLDLLPNGNLREPLSRLDSVDTVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            10879..11460
                     /locus_tag="WJK66_000430"
     CDS             10879..11460
                     /locus_tag="WJK66_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEZ7578193.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAEAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PAFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            11529..11711
                     /locus_tag="WJK66_000431"
     CDS             11529..11711
                     /locus_tag="WJK66_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEZ7578194.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARTLSEEELKA"
     gene            11708..12469
                     /gene="kdsB"
                     /locus_tag="WJK66_000432"
     CDS             11708..12469
                     /gene="kdsB"
                     /locus_tag="WJK66_000432"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEZ7578195.1"
                     /translation="MTEFVVLIPARLDSSRLPRKALADIHGKPMVVRVAEQAAKSKAA
                     RVVVATDHPDIQTVCQAHGVEVVMTSNRHESGTTRLAEASAALKLPPHLVVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYPRDAMRAGKREMPSETAVLRHIGIYAYRAGFLQRYAEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRVRAVFQTV"
     gene            12492..12809
                     /locus_tag="WJK66_000433"
                     /pseudo
     CDS             12492..12809
                     /locus_tag="WJK66_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            12914..13111
                     /locus_tag="WJK66_000434"
     CDS             12914..13111
                     /locus_tag="WJK66_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578196.1"
                     /translation="MAHGHKARADGLAQQPDKAAEANEEEYLTKALSQNLLSTLDVAL
                     ARFPEDAWFQEIKQDAQKHFA"
     gene            13268..13360
                     /locus_tag="WJK66_000435"
     tRNA            13268..13360
                     /locus_tag="WJK66_000435"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13302..13304,aa:Ser,seq:gct)
     gene            13785..14570
                     /locus_tag="WJK66_000436"
     CDS             13785..14570
                     /locus_tag="WJK66_000436"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEZ7578197.1"
                     /translation="MSRIRQAFAALDGGKALIPYITVGDPDIGATLAIMHSMVENGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAMAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESA
                     RKIGRVADAVIVGSRIVKEIENNAGNEAAAVGALVKELKDAVR"
     gene            14607..15479
                     /locus_tag="WJK66_000437"
     CDS             14607..15479
                     /locus_tag="WJK66_000437"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEZ7578198.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEVAGNVKPTDPLKFKDGKKYPDRLSAA
                     RKLTGEDDALVVMKGKMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGVRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(15539..16150)
                     /locus_tag="WJK66_000438"
     CDS             complement(15539..16150)
                     /locus_tag="WJK66_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEZ7578199.1"
                     /translation="MRRTALLLLALAAGTSLAAGFSQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNDKLSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGGVPADTKGLVQRLKERAGRYAPSIKPHDK"
     gene            16259..16483
                     /locus_tag="WJK66_000439"
     CDS             16259..16483
                     /locus_tag="WJK66_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEZ7578200.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVQHK"
     gene            16484..17089
                     /locus_tag="WJK66_000440"
     CDS             16484..17089
                     /locus_tag="WJK66_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578201.1"
                     /translation="MPSEMRMSRLMLLLSAFVLAACNAQNRQSGVRHQDFARVAVDLF
                     RSACVLTQGSFESVSKFAAVGHFVPVGKEDLSHLPPTVAEPDVQALWTLERDGGIYYL
                     SLTRDSCSVKAERADSAALLEHFDGLVRQPPKNANIELRAEQSVQASFEIRQVSYAWR
                     EAGSSEETVLTAQTADSPDLPVQAVLNLTHRSHNGKPLILP"
     gene            17149..18183
                     /gene="pyrC"
                     /locus_tag="WJK66_000441"
     CDS             17149..18183
                     /gene="pyrC"
                     /locus_tag="WJK66_000441"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEZ7578202.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDQATPALVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAATVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAIELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENTDTITLVKQSQTVPASIPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(18549..18974)
                     /gene="nusB"
                     /locus_tag="WJK66_000442"
     CDS             complement(18549..18974)
                     /gene="nusB"
                     /locus_tag="WJK66_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEZ7578203.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMPDFAKADE
                     ELFNKLFFGTQTNAAEYIRQIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(19052..19528)
                     /locus_tag="WJK66_000443"
     CDS             complement(19052..19528)
                     /locus_tag="WJK66_000443"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEZ7578204.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNFASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            complement(19575..19898)
                     /locus_tag="WJK66_000444"
     CDS             complement(19575..19898)
                     /locus_tag="WJK66_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HEZ7578205.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNQPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADITKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPKD"
     gene            20065..20784
                     /gene="rnc"
                     /locus_tag="WJK66_000445"
     CDS             20065..20784
                     /gene="rnc"
                     /locus_tag="WJK66_000445"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEZ7578206.1"
                     /translation="MKDDVLKQQAHAAIQKKLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFAERVRRVDFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVISCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            20831..21754
                     /gene="era"
                     /locus_tag="WJK66_000446"
     CDS             20831..21754
                     /gene="era"
                     /locus_tag="WJK66_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEZ7578207.1"
                     /translation="MDIETFLAGERAADGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRFTDADRVVLKQLPKHTPVILVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELIKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
BASE COUNT         4829 a         5359 c         6462 g         5210 t
ORIGIN      
        1 acgccgtact ggtttttgtt aatccactat aaaaaacact gcagcaaatc gtttaaaaac
       61 aagcgtcctt tttcggtcgg gcggaatacg gcggggtcgg tttccagcag gcctttttgc
      121 cttgccgttt cgatttgcgc catgattttg gcactcggta cgcccgtgcg ctcctgcaac
      181 atcgcggtgg gtacgccgtc ggtcaggcgc agggcgttca tcatgaattc gaacggcaaa
      241 tcttcggcgg cgacggtttt gcgttcgacg gcttcgctcg gtcggttttg cattaaggcg
      301 aggtagtcgt tggggtggcg gcggcggacg gtgcgctcga tgcggtcggg ataggaaatt
      361 ttgccgtgcg cgcccgcgcc tatgcctaaa taatcgccga actgccagta gttcaaattg
      421 tggcggcact gcatggctgg tttcgcaaaa gccgatgttt cgtagtggac aaaacccgcg
      481 ctttccagcg cgccgtgtac cgcgtcttcg atgtcgaggg cggcttcgtc ttgcggcaaa
      541 cctttcggcg gcgtatgacc gaacggcgtg ttcggttcca tcgtcagatg atacgcgctg
      601 atgtgggtcg cacccgtagc gatggcggtt tgtacgtcgt ccaatgccgt ctgaacggtt
      661 tggttcggca gggcatacat caagtcgata ttgactttat caaataattt caaggcggta
      721 tcgatagcgg ttaaggcttc cttaccgttg tggacgcgcc ccagccttga gagcatatcg
      781 tcgttgaaac tctgtacgcc aatagaaagc cgcgtgatgc ctgcgtcttt aaatccttga
      841 aacttctcga tttcaaacgt ccccggattg gcttccaacg taatttccgc ttccggctgc
      901 aagcgcaaca gcgaacgcac gccgctcaac aaacggtcaa tcgattccgc ctgaaacagg
      961 ctgggcgtac cgccgccgaa aaagatcgtt tccaccggtc tgccccaaat attgggcaat
     1021 tcaagctgca agtcggtcag cagcgcgtcg atataggcgg cttcgggcaa tccgtttttc
     1081 aggctgtggg aattgaagtc gcaatacggg cattttttga tgcaccacgg gatgtggatg
     1141 taaagcgaca ggggcggcag ggcggtgagt cgggtgcggt ttggaaagga aatggtgtgc
     1201 atggtgtggt tcggaaaagt ggacaatgcc gtctgaaggc ggttcagacg gcacaggttc
     1261 agccgagcag ggtaagcagt tcggcttcgc tgaggatgga aacacccaag gcattggctt
     1321 tttccagctt gctgcccgcg gcttctccag cgacgacgta atcggttttt ttggaaacgc
     1381 tgccggaaac tttgccgcct gcggcttcga ttagggattg ggcttggtcg cgtttgaggg
     1441 tgggcagtgt gccagttaac acgaaggttt tgcccgctac ggctttattg atgccgtctg
     1501 aaccttgcgc cgcctcgtct tcagacggca tttgcgcgaa gaaggttttc aggttttcga
     1561 gtagggcggc gttttgtggt tcgctgcgcc atgcttgcca gtcggcgggg agggctttgt
     1621 cgttttgtag accttctatg tttttaccag cgagttccca taaggcttgg gctttgtttt
     1681 cgctaatttt gaagtcgggc aggcgggcga tccagcgttg cggttcggcg tggcgggcgg
     1741 gcgggatggt aacggcttgg gtttgcgggg caacgcctgc ggcgagcagt tcgtctatca
     1801 tcgcctgctg ttcggcttgg gcgaagaagt gggcaatgga acgcgccact accgtaccga
     1861 tgtcgggcag gcaggcgagg acgggttcgg gtgcgcggcg gacgcgttcc aatgtgccga
     1921 atgcctgtgc cagcgttttg gcggtgcgtt cgccgacgtg gcggatgccg agcgcgaaca
     1981 ggaagcgcgc gagttcgggc gttttgccgg attctatgcc tgcgaggatg ttttccgccc
     2041 acttgaccgg ttgtttttta tgtttgcccg acgcgccgac cgaactgcct tcagacggca
     2101 tttgatccga ttcggcaacg gttttgtccg ccgtttcctt cattttttgc agggtcggaa
     2161 tatcgaggcg gtagaggtcg gcgaagtgga ggacgaggtc ttgggcgacc agctgttcga
     2221 tttgtttttc acccaagccg tcgatgtcca tggctttgcg cgaggcgaag tggattaagc
     2281 cttgcgcgcg ttgtgcctga caaagcatac cgccgctgca tcgggcgacg gcttcgcctt
     2341 cttcgcgttc gatttcgctg cggcagatgg ggcaatgggt cggcaggcgg tagggcttgt
     2401 ggagcggaac ggattgggtt tgatttgcag ccggtgcttc ggcaaacaaa tcgtcctgcc
     2461 gatgcccgat gccgtctgaa acggcaacgg cggtttcctg catcgggcgg cgttcaaaaa
     2521 tcacacgcac gacttcggga atcacgtctc cggcgcggcg cacgacgacg gtatcgccga
     2581 cgcgcacgtc tttacgcgat acttcgtcct gattgtgcag ggtggcgttg gtaacggtta
     2641 cgccgccgac gaatacgggt tgtaatcggg caaccggcgt taccgcgccc gtcctgccga
     2701 tttgcacgtc aatcgcttcg acaatggtca gggcttcttc ggcagggaat ttgtgggcaa
     2761 tcgcccagcg tggcgcgcgt gagatgaagc cgagttcgcg ctgttgcgcc aagctgttga
     2821 ctttgaccac cataccgtcg atttcgtagg gcagttcggg gcgtttttgc tgcatttgtt
     2881 cgtaaaacgc caatacttcg tcgatatttt tgaaacagcc gaaattgcca tttggcagac
     2941 tgaagccgag tgcttggaaa taggcgagtt cttggatgtg ttcttccgcg acgaaaccgc
     3001 cttgctggcg ggcgacggag taggggaaaa agtgcagttt gcgttgcgca gtgatgcgcg
     3061 aatcgagttg gcgcaggctg ccggcagcgg cgttgcgcgg attggcaaag ggtttttgcc
     3121 cgttttcggc ttgtctttta ttgagggcgg caaaatcggc tttttgcatc agtacttcgc
     3181 cgcgtacttc gatgagttcg ggcgtatttt cgccgtgcag ccgcaagggg atgttggata
     3241 cggttttgat gttttgggta acgtcttcgc ccgtcgtgcc gtcgccgcgc gttgccgcct
     3301 gtaccaatac gccgtcgcgg tagagcaggc tgatggcgag gccgtcgaat ttgggttcga
     3361 taacgtattc gggtttgccg ccgtccaaac cgtcgcgcac gcgttggtcg aaagcgtaca
     3421 tttcggcatg gtcgaacacg ccgttttcat cttgcgggga aaaagcgttg gtcagcgaca
     3481 gcatcggcac ttcgtggcgt acttcggcaa atcccgccaa aggctcgccg ccgacacgct
     3541 gggtcgggct gtcgggcagc ttgagctcgg gatggtttcg ttccaacgct tcgagttcgc
     3601 ggaataattt gtcgtattcg gcatcgggta cgctgggcgc gtcgagggtg tagtattcgt
     3661 aggcatagcg gttgagcagg tcggtgaggt ggcggatgtg ttgtgcggca aattgtttta
     3721 tatcactatc agacggttta agaagattgg taaagttagt gttatgtttt gagtttggat
     3781 tcatgagaga aggttttcag acgacctttg tctgatacgg gatgaaacgg gcaaaggtcg
     3841 tctgaaaaat gataggttga aaacagctga attttacccg aaaaaaagcg gatatgccgt
     3901 aacgacatat ccgctttgat tgcattcgat tttaggagaa caggcgcagg gcggttttgc
     3961 cgcccggttc gataccgact ttgagcatct cggactgacg cgccaataca taagtgcgta
     4021 cgtctttgag ccattgggtc gaaacttctt ccattttgtc gttgaccaga ttcaggttca
     4081 actggccgga caggcgtacc gccaaatcca taaacaaatc gtcgaaggtt ttttcgcctg
     4141 ccggagagtg cgggatgtcg agcagcatac tgaagccttt gtaggactgg ttgtccaaaa
     4201 gggcattggt aaacggctcg ttgttgagcg agcagatgga gaacatggtc gagcccgacg
     4261 tgtcggtata gtggaacgcg ccgtcgtctt ccaaaacgaa acccacgccc gttacggcgg
     4321 aacgcagttc tacgccgctg atgctggtcg gggaaaccaa atggatggcg atggtctggt
     4381 cgacgcgcgc gcagaatgcg tccagcgcgg aagccacttc ggtaaaggcg gcaaggtcgg
     4441 tatgcagcgt ctgaccgccc atgctttgtg cgaatgcgtc cacctggcgg ttgaaggcgg
     4501 agagttcttc ctgcgaggca agtccgttgc ggctgactgc ctgaataccc acgataaatg
     4561 cctgatagcg gatgcccggg atgggttcgg caatctggaa atggtcgtcc atggtgcagc
     4621 cgacaatttg gtagcggcag cggttggaaa ggcgcggcag tgcgtgcagt tctttggctt
     4681 cggtcagcgc gatataagag atgaagtcga agcgcacgtc aaaccagggc agctcgacct
     4741 tcgacagctc tttgagcgta atcagcggtt ttgccggcgt ttgcggaacg ggaacgggtt
     4801 ttgccggcgc gtcggcaggt ttcggtgcgg aatgtccggt ttggggttcg gaaacggtgt
     4861 gggcggagtt gccgataatg ccgctttctt ccaaggcggt ttcgatttcg gttttgaacg
     4921 gggaggcttt tgcctgtttc tgcttggcga tgtagacggc atcctgttct tgcaggttgc
     4981 gcatggcggg gtcttggggt tttgccgttt ttttgaccgc cggttggggt ttcggcatca
     5041 tgactggccc gccggacggt ttgccgtcgc ggacatggct ggttttgctg ttgagcaggg
     5101 catctttgtc ggagtgcccg aactggtcgc gcactttttt gcggtattgg ttttcctgat
     5161 acatattgta ggcgacaacg gcgaggacgg cggcgaggaa cagtacgatg taaatcatgg
     5221 caatcacttt aaatttcggg atgcaggatg cgcaaagtgc gggtactgcg gttaaatcgg
     5281 gcttgcgttt ccggcagtct gacggaacgg ccggttataa cgtttgaatt ataacgaaaa
     5341 ttgcagggtc tgacagcagt gtgtcgaaat aagcggaaat tttccgaaat gccgtctaaa
     5401 atctgtggtt ttcagacggc atttctgtcc acgggaaacc ctttcccccg tatccgccgc
     5461 cagtcgaaaa aatgaccggg gtcggttttg cggccgggcg cgatgtcttg gtgccccgtt
     5521 accgccgtga tggggtagcg gcggcagatt gcgtccaaca aggcttcgag cgaacggtat
     5581 tgcgcttcgg taaagggttc gaaatcgcag ccttccagtt cgatgccgat ggaaaatgcg
     5641 ttgcattttt cccgtccgcg aaacgaggat acgccggcat ggtatgccat attgtcgcag
     5701 gaaacgaact gtaccgtttc gccgtcgcgt ttgattaaaa aatggctgga tacgcgcaaa
     5761 gtgtgtatca ggctgaagaa cggatgtccg tcggggtcga gccggttggc aaacagcttt
     5821 tccaccgcat ccgtaccata ttcgaacggc ggcagcgaga tattgtgcaa tacgacaagg
     5881 gaaacctctt ctttggactc cctcgggccg aaattcggcg acggaatgtg gcgcacgctt
     5941 tgcagccaac cgtcctgcca gggtgccgta gcgggattgt ccatgatgtc ctttctgtct
     6001 tatggggtaa tttgggttat actgtcgccc gaattttaag atgtattccg atattgggaa
     6061 tcctaccatg ttgagaaaat tgttgaaatg gactgccgtt tttttgaccg tatcggcagc
     6121 cgttttcgcc gcgctgcttt tcgtccctaa agacaacggc agggcatacc gaattaaaat
     6181 tgccaaaaac cagggtattt cgtcggtcgg caggaaactt gccgaagacc gcatcgtgtt
     6241 cagcaggcat gttttgacgg cggcggccta cgttttgggt gtgcacaaca ggctgcatac
     6301 ggggacgtac agattgcctt cggaagtgtc tgcttgggat atcttgcaga aaatgcgcgg
     6361 cggcaggccg gattccgtta ccgtgcagat tatcgaaggt tcgcgttttt cgcatatgag
     6421 gaaagtcatc gacgcaacgc ccgacatcga acacgacacc aaaggctgga gcaatgaaaa
     6481 actgatggcg gaagttgccc ctgatgcctt cagcggcaat cctgaagggc agtttttccc
     6541 cgacagctac gaaatcgatg cgggcggcag cgatttacgg atttaccaaa ccgcctacaa
     6601 ggcgatgcaa cgccgcctga atgaggcatg ggaaagcagg caggacgggc tgccttataa
     6661 aaatccttat gaaatgctga ttatggcgag cctggtcgaa aaggaaacgg ggcatgaagc
     6721 cgaccgcgac catgttgctt ccgtcttcgt caaccggctg aaaatcggta tgcgcctgca
     6781 aaccgacccg tccgtgattt acggcatggg tgcggcatac aagggcaaaa tccgtaaagc
     6841 cgacctgcgc cgcgacacgc cgtacaacac ctacacgcgc ggcggtctgc cgccaacccc
     6901 gattgcgctg cccggcaagg ccgcgcttga tgccgccgcc catccgtctg gcgaaaaata
     6961 cctttatttt gtttctaaaa tggacggtac gggcttgagc cagttcagcc atgatttgac
     7021 cgaacacaat gccgccgtcc gcaaatatat tttgaaaaaa taaaccatgc cgtctgaaaa
     7081 gtttgtgttt tcagacggca tacccttacc ggaactgcaa gcatgaaacc gcaattcatc
     7141 actttggacg gcatagacgg tgccggcaaa tccaccaacc ttgccgtcat caaggcatgg
     7201 tttgaacgga gggggctgcc cgtgctgttc acgcgcgaac cgggcggaac gccggtcggt
     7261 gaggccttgc gcgaaatcct gctcaaccct gaaaccaaag ccggtttgcg tgcggaaaca
     7321 ctgatgatgt tcgccgccag aatgcagcat attgaagacg tgatattgcc cgcgctttca
     7381 gacggcatac acgtcgtatc cgatcgtttt accgatgcga ccttcgccta tcagggcggc
     7441 gggcggggga tgccgtctga agacattgaa attttggaac attgggtgca gggcggtttg
     7501 cgccccgatt tgaccctgct gttggatgtg ccgctggaag tgtcgatggc gcgtatcggg
     7561 cagacgcgcg agaaagaccg attcgagcag gaacaggcag atttcttcat gcgtgtgcgc
     7621 agcgtttatc tgaaccgtgc cgccgcctgt cccgaacggt acgccattat cgacagtaac
     7681 cgcagcttgg atgaagtcag aaacagcata gaaaaagtgt tggacggaca tttcggctgt
     7741 taatgcggca aatattgaaa caagcgcatc cacccgcgcc gaaaatcaaa cggcagtgcc
     7801 gcaggtgaaa atggcggtat gcgccaaact ttcggcatga tagaattacg ctcggttaca
     7861 aggcaggatg cgtcggcaat attaacgaac cgcccgtaac atgatgaccc gaaagcgttt
     7921 cggacagtcc gattcaaatc tttttctcgc aacaggattg acacatggaa aactcattga
     7981 aagaagccgc cctcaagttc cacgaattac ccgttccggg caaaatttcc gttaccccga
     8041 ccaaatcttt ggcgaccgac aaagatttgg cgttggcgta ctctccgggc gtagccgctc
     8101 cttgtatgga aatccatgcc gatccgcaaa atgcctacaa atacaccgcc aaaggcaact
     8161 tggtcgccgt catttccaac ggtacggccg ttttgggctt gggcgacatc ggcgcgctgg
     8221 cgggcaaacc cgtgatggaa ggtaaaggcg tattgttcaa aaaattcgcc ggtgtggacg
     8281 tgttcgacat cgaaatcgat gaaaaagacc cgcaaaaact cgtggacatc atcgccgctt
     8341 tagagccgac cttcggcggc atcaacctcg aagacatcaa agcacccgag tgtttctaca
     8401 tcgaacgcga attacgcaaa cgctgcaaaa tccccgtatt ccacgacgac cagcacggca
     8461 cggccatcat taccgccgcc gccgtattga atgccctgcg ttttaccggc cgtaaaatcg
     8521 aagaagcgac tttggtgtgt tccggcgcag gtgcggccgc gattgcctgc ttgaaccaat
     8581 tgctggattt gggcttgaaa cgcgaaaacg tgaccgtttg cgactccaaa ggcgtgattt
     8641 accaaacccg cgaagacaaa gaccgcatgg atgagtctaa acagttctac gccattgaag
     8701 acaacggcca gcgcgtgctt gccgatgccg tcaaaggcaa agacatcttc ttgggcctct
     8761 ccggcgcgaa cctgctgacg cctgaaatgc tgaacaccat gaacgaaaaa cccatcgtgt
     8821 tcgccatggc caacccgaat ccggaaattc tgccgccgct ggcaaaagaa acccgtccgg
     8881 acgtggttat cggtaccggc cgttccgact tcccgaacca agtcaacaat gtattgtgct
     8941 tcccgttcat cttccgtggc gcgttggatg tcggcgcgac caccatcaac gaagaaatga
     9001 aacgcgcctg cgtgtatgct ttggcggatt tggcgatgga agaagtgacc gaagaagtgg
     9061 ttgccgctta cggtaagaaa tttgaattcg gcgcggaata cctgattccg actccgttcg
     9121 attcccgcct gctgccgcgc gtcgctacgg ctgccgccaa agcagcgatg gaaagcggcg
     9181 ttgcaacccg tccgattgcc gactttgaag cctacgccgc caagctgggc gaatggaagc
     9241 tgtaagccgt ttgcggttta aaatgccgtc tgaactgttt tcaggcggca ttttgctgtc
     9301 agattgataa atgaaagata ctggaaaatg aaagagatga aacctgtccg ttatcatatt
     9361 ggcgatatgc ccgagacttc aaaacaaacc gcctcccgtc atgacgacag ggcagtgggt
     9421 gttgacgatg attgttttca tgattccttt ggtcaatttt tgtttgggtg ttcggcagag
     9481 gcaacccgaa ccgcgccaat ttctgtaaag cgcagttgct tatttacctg attggttcgc
     9541 ttatcggttt ggtcttcgcg ttgtttatag gtgggtctgt atcaggtacg catgattaat
     9601 gccccgggct gattttgctt cgaggatttg tatcgaatat gccgaattgt ttcaaatttc
     9661 ataccgttat cgaacggcat tggcaaaaac cttatccggt tttgtctttt ctgcttaagc
     9721 cgctctccgg gctgtttgcc aaaattgcgg caaaacggcg gacggatttt ttatcgggaa
     9781 aacggcaaag cgaaaagctg cccgtgcctg tggtcgtggt cggcaacatc cacgcgggcg
     9841 ggacggggaa aacgccgatt gttgccgcgc tggtgtcggg tttgcaggaa aagggcgtca
     9901 aggtcggcat catcagccgg ggctacgggc gcaagagcaa ggcggttcat gtattgaatg
     9961 ccgcaagccg agcggaagat gcgggcgacg agcctttgct gctgttcagc aaaaccggtg
    10021 cgccgacggc ggtgggcagc agccgtgcag aggtaggcag ggcattgctg gcggcgcatc
    10081 ccgacatcgg actgattgtg gcggacgacg gtttgcagca ttacgccctg cggcgagatg
    10141 tggaaatcgc ggtgtttccg gcggcggata cggggcgcac ggatttggat ttgctgccca
    10201 acggcaattt gcgcgaacct ttgtcgcggc tggattcggt ggatacggtc gtcgtcagcg
    10261 gcggcaaggc ggatgcgctg tttaggccgt ctgaaaatat gtttcacagc cgtatcgaag
    10321 cgggacgaat ttaccgtttg aacaatccgt ccgaaatact ggacacaggc cgtctgaaaa
    10381 atcaaaccgt cgtcgccgtg gcaggtattg ccaagccggc gcgttttttc gattcgttgc
    10441 ggaatatggg cattaccgtg aagcgaaccg tcgcgctgcc cgaccacgcc gacatttcgg
    10501 cggcagattt gcccgatgcg gacgcggtca ttattacgga gaaagatgcg gtcaaatttt
    10561 cagacggcat ttgcaccgat aatgtttggg tgttgcccgt ttgtgcgata atcgaacctg
    10621 atttggcggc gtttgtgttg gagcggttgg aagatgtacc gaaggccgtc tgaaagcacg
    10681 gtttgggcgg agtgattacg gatttgaata agaacgcctc gcgccgtcat tcccgcgcag
    10741 gcgggaatct aagtctcgaa ttttcaggaa tgcctaggag gcttcagaaa tcccaaatct
    10801 ccggatttcc acttggacag gaatgagaaa atcggtcgta ttttttatct gcattaatca
    10861 ttcgttaaag gattgaatat taaactgaaa accttgttat tgcccttcgc cacgctggca
    10921 ttgtgcacca acgcttttgc cgccccgccc agcgacgcgt cgttggcgcg ttggctggat
    10981 acgcagaatt ttgaccggga tatagaaaaa aatatgattg agggctttaa tgccggattt
    11041 aaaccgtatg cggacaaagc ccttgccgaa atgccggaag cgaaaaaaga tcaggcggca
    11101 gaagccttta accgttatcg tgagaatgtt ttgaaagatt tgattacgcc cgaagtgaaa
    11161 caggctgtcc gtaatacttt attgaagaat gcccgtgaga tatacacgca agaagaaatt
    11221 gacggcatga ttgcctttta cggttcgcct gtcggtcagt ccgtcgttgc caaaaatccg
    11281 cgcttaatca agaaatcgat gagtgaaata gcagtatctt ggactgcatt gtcagggaaa
    11341 atcgcgcgac atcatctgcc cgcgtttacg gaagagttgc ggcgcatcat ctgcggtggt
    11401 aaaaatcccg atgcgggctg taaacaagcc ggacaggttg ggaaaaggca tcagaaataa
    11461 atgatagccg tctgaaatat tgaagagggc atccgattga ttgaaccatc aaacccgaaa
    11521 gcaaccctat ggaaaaaaaa ttcttagaca tcctcgtctg ccccgttacc aaaggcaggc
    11581 tggaatatca tcaggacaaa caggaattgt ggagccgtca ggcgaagctt gcctatccga
    11641 ttaaagacgg cattccctat atgctggaaa acgaagcgcg aacgttgagc gaagaggaac
    11701 tcaaagcatg accgaattcg tcgtattgat tccggcgcgg ctggattcgt cgcgcctgcc
    11761 cagaaaagcc ttggcggaca tccacggcaa accgatggtc gtgcgcgttg ccgaacaggc
    11821 ggcaaaaagt aaagctgcgc gcgtcgttgt tgccaccgac catcccgata ttcagacggt
    11881 ctgtcaggcg cacggcgtcg aagtcgtcat gacttcaaac cggcacgaaa gcggcacgac
    11941 gcgccttgcc gaagcctctg ccgcgctgaa gctgccgccg catctggtgg ttgtcaacgt
    12001 acagggcgac gagccgctga ttgcccccga actcatcgac cgcactgccg aagtcttggt
    12061 ggaaaacaac gtacagatgg caactgcggc acacgaattg cacgatttcg acgaattgat
    12121 gaatcccaat gccgtcaaag tcgtcctcga caaaaaccgc aacgccatct acttcagccg
    12181 cgccccgatt ccctatccgc gcgatgcgat gcgtgccgga aaacgcgaaa tgccgtctga
    12241 aaccgccgtc ctgcgccata tcggcatcta cgcttaccgc gccggcttcc tgcaacgcta
    12301 tgccgaaatg agcgtttcgc cgctggaaac catcgaatcg ctggaacagc tgcgcgtcct
    12361 gtggcacggt tatcccattg ccgtcgaaac cgccaaagaa gcccccgccg ccggtgtgga
    12421 tacgcaggaa gatttggata gggttcgcgc tgtatttcag accgtataaa acaggttcaa
    12481 agggaaaaga tatgcagcaa catattgaaa agtggcaaca cttgagccgg gaagaacaga
    12541 aaatccttgc cgaagtatgg ggtctcgtgc aaaacgatga tcaggaggtt cactatgaaa
    12601 tgcttaaatt gaacgcaccc gacgaagcca gtggcgaatt ttggttcaga atggcagaaa
    12661 cactcagcac cctgccgccc aaccgttccc tcgaccttag aatgaacggc ggcaggctgg
    12721 cgaccgccgt atccatcctt tccgtcatga ttgaagacaa tcccgacata ccgcagcttt
    12781 gggcgcaaaa aattaccgcg ctcaattata gtggattaaa tttaaaccag tacggcgttg
    12841 cctcgccttg tcgtactatc tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt
    12901 taatccacta tatttggcac acggacacaa agcccgtgcc gacggtttgg cacaacagcc
    12961 cgacaaagcg gcagaagcca acgaggagga atacctgacc aaagccctgt cgcaaaacct
    13021 gctgtcaaca ttggatgtcg cgcttgcacg ttttcctgaa gacgcgtggt ttcaggaaat
    13081 caaacaggat gcacaaaagc attttgcttg aggatgtggc agtcaggaat atttccattc
    13141 aggaagaaaa gaagtgcctg attgggtata atcagggtaa atcttatttt atttcaaaag
    13201 attaatattt gctttctgtt tttccttgac ggtatcggaa aagttgatta tagttacagc
    13261 ttccttagga gtaatggctg agaggctgaa ggcacttccc tgctaaggaa gcatgtgggg
    13321 tcaacctgca tcgagggttc gaatccctct tactccgcca gataaaaaat agacgctgtg
    13381 ttttacagcg tctatttttt atgcaatttt atagcgggtt ggtgcaaaac cagtatggta
    13441 ttgccgtgtc ttgattctga attttgttat agtggattaa caaaaaccag tacggcgttg
    13501 cctcgcctta gctcaaagag aacgattctc taaggtgctg gagcaccaag tgaatcggct
    13561 ccgtactatt tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt taatccacta
    13621 taatccgaga tgcttgccgt ttatttccgc ctcgttcaaa cggcggctct gatttgcgcg
    13681 gtttctgttt gccgtattcg cctatccgta ccgcaaatgt tatactggga aaaatttact
    13741 gattgtgttt tacggcatat ttgccgatag gatggaagag acaaatgagc agaatccggc
    13801 aggcttttgc cgctttggat ggcggaaagg cattgattcc ctatattacg gttggagacc
    13861 cggatattgg ggcgactttg gcaataatgc acagtatggt ggaaaacggt gcggatattt
    13921 tggagttggg cgtgccgttt tccgatccga tggcggatgg accggttatt cagcgtgcgg
    13981 cggagcgggc gttggcgaac gggatttcgc tgcgcgatgt cttggatgtc gtcagaaaat
    14041 tccgtgaaac cgacacgcaa actccggttg ttttgatggg atatttgaac cctgtacata
    14101 agatgggtta tcgggagttt gctcaggaag ccgcaatggc gggtgtggac ggcgtgttga
    14161 cggtggattc ccctgtcgaa accatcgatc cgctctatcg cgagctgaag gataacgggg
    14221 tcgactgtat tttcctgatt gcgccgacga cgacggaaga ccgtattaaa accattgccg
    14281 agctggcagg cggatttgtc tattatgttt cgctcaaggg cgtaacgggc gcggcaagtt
    14341 tggatacgga tgaggtttcg cgtaaaatag agtatttgca tcagtatatc gatattccca
    14401 tcggtgtcgg tttcggcatc agcaatgcgg aaagcgcgcg caaaatcggc cgggttgccg
    14461 acgcggtcat tgtcggcagc cggattgtga aagagattga aaacaatgcg ggcaacgagg
    14521 ctgcggccgt cggtgctttg gtcaaagagt tgaaggatgc cgtgcgctga ttctgtcacg
    14581 cattctaaat attttaggag ttgtccatga gctggttaga taaaatcctg ccacccaaaa
    14641 tcaaaaaccg cagcaaagac ggttcttcca atgttcccga gggtttgtgg cacaaatgtc
    14701 cgtcttgttc ggcaaccgtt tattcgaccg agttgcagca gaacaatcag gtttgtccga
    14761 aatgcaacca ccacaatccg ttgtcggcac gacaacgcct gaatctgctt ttggatgagg
    14821 atggcagaga ggaggttgcc ggaaatgtca aaccgacaga tcctttgaag tttaaagacg
    14881 gcaaaaaata tccggatcgt ttgagtgcgg cacgcaagct gaccggggaa gatgatgctt
    14941 tggtggtaat gaaaggcaag atgaacggtc tgcccgttgt ggttgctgcg tttgagttcc
    15001 gctttatcgg cggttcgatg ggttcggttg tgggcgaacg attcgtacag ggcgtccgcc
    15061 gggcggttgc cgacaattgt ccgttcgtct gtgtggcggc ttccggcggt gcgcgtatgc
    15121 aggagggtgt gaactcattg atgcagatga cgaaaaccag tgccgcgctg catttgctga
    15181 cggaaaaacg cctgccgttt atatcggtgt tgaccgatcc gactatgggc ggcgtatccg
    15241 ccagcttcgc atttttgggt gatgttgtgc ttgccgaacc gaacgcgctg atcggttttg
    15301 ccggtccgcg cgtgattgag cagacggtgc gcgaaacgct gccggaaggc ttccaacgcg
    15361 ccgagttcct gctggaaaaa ggcgcaatcg accagattgt cgaccgccgc gatatgaagc
    15421 ggcgcatcag tgatttgatt acgctgttgt gccgtcagga caaagtttct gccgcctgat
    15481 ggctgatgaa tcgaataccg tctgaaaccg atgtttcaga cggtattttt gtgtctggtt
    15541 atttgtcgtg cggctttatc gacggggcat agcgtccggc acgttctttc aggcgttgta
    15601 ccaaaccttt cgtgtcggcg ggtacaccgc cctcgcagaa tgcctgatac aggacggtgc
    15661 gcagtgcgtc gttgcggctt aatgtaccgc ctatcggttt ccattcggcg tttcggggct
    15721 gtatccagcg gcggttgacc gtgtcgccgt agccgaacac tttgtaggag gaaagtttgt
    15781 cgttgccgaa cctgatggag aagcgggcgc agaatatgcc ttcggcagtc aggttgtcgt
    15841 agcctttgtc ggagcggata ttgagaatgt agcggatgct gccgtcgggc gcgggcataa
    15901 tttgcaggct gtcgagcagg attttcggct gtttgccgta attttcatcc acataaatgt
    15961 cgaaccagcc gtccgagtgc gtatcgggca gcggcggcag tttggcggta tgttctttaa
    16021 attcgcgggc ggcggcctct tcgggcgttt cgcggtagcg ggtgttgatc ggcgtgtctt
    16081 tttggctgaa gccggcagcg agggacgtgc cggcggcgag tgcgagcagc aggagggcgg
    16141 tgcggcgcat aagtttcttc aaattgaaaa cggcgttatt ttatgggttg gcaaaggggg
    16201 ctgcaagcaa ctggggtata atctcccccc gattcccatt ttttaaacgg tacaaacgat
    16261 gaacagcgaa actttagacg taaccggatt gaaatgtccc ctgccgattt tgcgggcgaa
    16321 aaaggctttg gcgcaaatgc agcagggcga cgtgttgacc gttctggcaa ccgacggcgg
    16381 cgcaccgggg gattttgagg ctttttgccg ccaaaccggt catgtgctgt tggatgcttc
    16441 cgaacaggac ggcgtgttca cgctggtcgt ccaacacaaa taaatgccgt ctgaaatgcg
    16501 gatgtcccga ttgatgttat tgctgtcggc atttgtgctt gctgcgtgta atgcccaaaa
    16561 ccggcaatcc ggtgtacggc atcaggattt cgcgcgtgta gccgtcgatt tgtttcggag
    16621 tgcctgcgtc ctgactcaag gcagttttga atcggtttcc aagtttgccg ctgtcggaca
    16681 ttttgtccct gtcggaaagg aagacctgtc ccatctgccg cccactgttg cagaacccga
    16741 tgtacaggcg ttgtggacgc ttgaacgcga tggcggcatt tattatttga gcctgacgcg
    16801 cgacagttgc agtgtgaaag ccgagcgggc agacagtgcc gcgctgttgg aacattttga
    16861 cggacttgtc cgacagccgc cgaaaaatgc caatatagaa ttgagggcgg aacagtcggt
    16921 gcaggcatca tttgaaatcc gccaagtcag ctatgcctgg cgggaagcgg gcagctccga
    16981 agaaaccgta ttgaccgcac aaaccgccga ttccccggat ttacccgtac aggcagtttt
    17041 gaacctgacg caccgttccc acaacgggaa acccctaatt ctcccctaac ttcagacggc
    17101 ataaagtcgg catgccgtct gaacattcaa ataaaacaaa ggaaaaccat gcaaaccctg
    17161 accattatcc gccccgacga tatgcacctg cacctgcgcg acggcgacgc gctcaaagcc
    17221 gttgctcctt ataccgcccg ccaaatgggg cgtgccgtca ttatgcccaa cctcaaaccg
    17281 cctgtcgtca gcgttgccga cgcgcttgcc tacaaagcgc gcatcatggc ggcattgccc
    17341 gaaggcagcg cgtttgagcc gttgatgacg ctttacctga ccgaccaagc cacgcctgca
    17401 cttgtgcgcg aagccaaagc cgccggcatc gtcgccttca aactctaccc tgccggcgcg
    17461 accaccaatt ccgattccgg cgtaaccgac ctgttcaagc tcatccccgt gttggaagaa
    17521 atggcgaaac agggcatttt gttcctcgtt cacggcgaag taaccgaccc cgaaatcgac
    17581 atcttcgacc gcgaagccgc ctttatcggg cgcgtgatga aacccgtttt ggcgcaagtg
    17641 ccgaatctta aagtcgtgtt cgaacacatc accaccgccg aagccgcccg cctggttttg
    17701 gaagcaggcg acaacgtagc cgccaccgtt accccgcaac acctcctgct caaccgcaac
    17761 gacctcttgg tcggcggcgt gcgcccccat catttctgcc tgcccgtact caaacgcgaa
    17821 acccaccgtc aggcattggt tgccgccgtt accggcgaga aggcgcataa attcttcctc
    17881 ggcaccgact ccgcgccgca cgccaaatcc gccaaagaaa acgcctgcgg ttgcgccggt
    17941 atgttcagcg cgatgaccgc catcgagctt tatgccgaag tgtttgaaaa agcaggcgcg
    18001 ttggacaaac tcgaagcctt cgcctcgaaa aacggcgcaa ggttttacgg cattcctgaa
    18061 aataccgaca cgatcaccct cgtcaaacaa agccaaaccg tccccgcaag tattccctac
    18121 ggcgacggcg aacttgtccc gatgcgcgcg ggcggcgaaa tcggctggac ggtacagtat
    18181 tgatggggct ggaaacaaaa tgccgtctga aaggtttttc agacggcatt tgtgttgttg
    18241 actgattgat acgtcaacgg cgtttgagtt tgttacgttt cggttatttc cgataaattc
    18301 ccacaatttt caaatttcgc cattcccacg aaggcaggaa tccggaaatt cgatgcgacc
    18361 agagtttatc aaaaacggca gcaactcaaa aaaccggatt cccgcctgcg cgggaatgac
    18421 gagattgaag tttcagaatt tatttgaaat acccaaaatt caaaaaacca aattcccacc
    18481 tgcgtgggaa tgacgaaaca aagaaagcag aaataaggac atagaacttt ctttaaattt
    18541 gtgatgcatc aacggcgttt gggctcgtcg ggacggattt gggcggcgag tttgtcgagg
    18601 atgccgttga cgaatttgtg cccgtccgtg ccgccgaagg ttttggttac ttcgatggct
    18661 tcgttgatga tgacggggta gggcgtttcg ggcatggcgg acagctcgtg gcaggcggtc
    18721 agcaggacgg cgcgttcgat ggggttgagg tctttttcgt cgcggtcgag cagggggcgg
    18781 atttgtcgga tgtactctgc cgcattggtt tgcgtgccga agaaaagttt gttgaacaat
    18841 tcttcgtctg ccttggcaaa gtcgggcatt tcgcggatgt ttttagcaat ctcgggcgcg
    18901 gcggtgcggt tgataaggga ttggtaaacg gcttgtacgg caagctcgcg ggaacggcgg
    18961 cgggctgttt tcatgatttt tccttgaaac ggttgggcgg cacggtatgc cgtctgaaac
    19021 ggaaagggcg tatcggtgta cgccctgttt gttattcttc gtcttcaaac tgttcttcga
    19081 gcaggaggtt gacgaggttg gcgcattcta cggcgacttt ggcggcatcc gaggcttttt
    19141 cttcaatccg ttcgattgcc tgtgcgtcgt tttccgtggt caggacggca ttggcaatcg
    19201 ggatgttgta gtcgagtgcg acgcggctga cccctgctcc ggattcgttg gaaaccagct
    19261 cgaaatggta ggtttcgcca cggataacga cgccgatggc aatcagtgcg tcaaattttt
    19321 cagaagaggc aaagttcatc agcgcgatgg ggatttcaag cgcgccgggt acggtggcga
    19381 cggtaatgtt ttcgtctgcc acgcccaatt cttggagggt gcggcagcag actttgagca
    19441 tttcgctgcc gatttcgttg gtgaagcgtg cctgtacgat gccgatgcgg aggtgtttgc
    19501 cgtcgaggtt gggggcgatg gtgttcattg agtgtccttt ggtattcgga ggtttcggaa
    19561 tgccgtctga agggtcagtc cttaggttgc cagtcggcga cggtttggaa tgtgccgtct
    19621 tctgccaatt cccacgcgct gccttcaggt tgggagagca gtgcggcggt ttcagggttg
    19681 gttttggtga tatcggcgag gctgacgatg ctgaagttgt ccgggtcgtc ggtgtattcg
    19741 tcggtctcgt cgccgctgaa gaaacgccag ccgctgtcgt tttcaaaaac gggggcttcg
    19801 cggtagagga agccgacggg ctggttttgt ttggcgacgg tgttggtggc gatgcagcgg
    19861 tcgagtgccg aggaaagtgc ttgtgcaaat gcgttcattg cgggaatacg ttgggggaaa
    19921 acttgcggat tttaccacga ttcttgcgtt gtcggcagac ggcggcggtt tggtggtaca
    19981 atgtgcgccg tttgcagcct taaggtgttt ctgtattttt ggagtatgga aacgcattcg
    20041 ggctgttttt tgcggaagac ggtaatgaaa gacgatgttt tgaaacagca ggcacacgcg
    20101 gcgatacaga agaaactggg ctacgcgttc cgcgatattt cgcttttgcg gcaggctttg
    20161 acgcacagga gccatcatgc gaagcacaac gagcggttcg agtttgtcgg cgattcgatt
    20221 ttgaattata cggtggcgcg gatgctgttt gacgcgtttc cgaagttgac cgagggcgag
    20281 ttgtcgcggt tgcgggcaag tctggtcaat gagggcgtgc tggcggaaat ggcagcggaa
    20341 atgaatgtcg gcgacggcct gtacttgggg gcgggcgagt tgaagagcgg cggcttcaga
    20401 cggccttcga tactggcgga cgcgatggag gcgatgtttg cggcggtcag cttcgatgcg
    20461 gatttcaaca cggcggaaaa ggtggtgcgc catttgtttg cagaacgcgt ccggcgagtc
    20521 gatttccaaa atcaggcaaa agacggcaaa actgctttgc aggaggcgtt gcaggcgcgc
    20581 cgtttcgcct tgccgaaata ccgtatcgaa gagcaaatcg gccatgccaa cgacagtatg
    20641 tttgtcattt cctgcgattt gggcgaactg ggtttcgtgt gccgtgccaa agggacgagc
    20701 cgcaaggcgg cggagcagga agcggcgaaa gaggctttga aatggctgga agagaagctg
    20761 ccgctgaaga agaaaaagaa atgaggcggc gcgtgaatat gccgtctgaa atatggatac
    20821 gaaagcaaat atggatattg aaaccttcct tgcaggggaa cgcgccgccg acggataccg
    20881 ttgcggcttc gtagcgattg tcggccgtcc gaatgtcggc aaatcaacgc tgatgaatca
    20941 tctcatcggt cagaaaatca gtattaccag caaaaaggcg cagacgacgc gcaaccgcgt
    21001 aacggggatt tataccgacg ataccgcgca gtttgtgttt gtcgatacgc ccggttttca
    21061 aaccgaccac cgcaacgcgc tcaacgaccg tttgaatcaa aacgttaccg aggcactcgg
    21121 cggcgtggat gtggtggttt tcgtcgtgga agcgatgcgt tttaccgatg ccgaccgcgt
    21181 cgtgttgaaa caactgccca agcacacgcc ggtcatttta gtggtcaaca aaatcgataa
    21241 ggacaaggcg aaagaccgtt acgcgctgga ggcgtttgtt gcccaggtgc gcgccgaatt
    21301 tgaatttgcg gcggcggagg cggtcagcgc gaaacacgga ttgcggattg ccaacctgtt
    21361 ggagctgatt aagccgtatc tgcccgaaag cgtgccgatg tatcccgaag atatggttac
    21421 ggacaaatcg gcgcgttttt tagcgatgga aatcgtgcgt gaaaaattgt tccgctattt
    21481 gggcgaggaa ttgccttatg cgatgaacgt cgaagtggag cagtttgaag aggaagacgg
    21541 tttgaaccgc atctatatcg ccgttttggt cgataaggaa agccaaaagg cgattttaat
    21601 cggcaaaggc ggggagcgtt tgaagaaaat ttccaccgaa gcgcggttgg atatggaaaa
    21661 actgtttgat accaaagtat ttttgaaggt ctgggtcaaa gtcaaatccg gttgggcgga
    21721 cgacatccgc ttcctgcgcg agctgggttt gtagtttttc ttgctgaact ttacgcaaat
    21781 gccgtccgaa caggtttcag acggcatttt gtttcaatcg ggaatatctt tgttaaaaac
    21841 gggttgatat tatctgtgca
//
LOCUS       DAWKOG010000015        21790 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.015, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000015 DAWKOG010000000
VERSION     DAWKOG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21790
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.015"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(161..532)
                     /locus_tag="WJK66_000447"
     CDS             complement(161..532)
                     /locus_tag="WJK66_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578208.1"
                     /translation="MRFYIDNKECDYIFFRKAVNISDDFIQSGFITPEGLITKNSNPK
                     LFNQYSKMVSKNKCQYEMVTFLSDEMKNIIELLSNDDAYIEFAYSEDFYVLPEIEIDK
                     RTLKSLNFYIDFGVKYIINKI"
     gene            complement(666..1109)
                     /locus_tag="WJK66_000448"
     CDS             complement(666..1109)
                     /locus_tag="WJK66_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578209.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQTFENQVFHTQADAPMQLA
                     ELSQKEMKETEGAAAPLVAIGILHAGRFLAQRWVTQRVAAQALSRGANVYARTSQQAR
                     AVANQAWGRQNVIRHGQKEIHSNYSHFQNSSQKIRGHAFYGNKHR"
     gene            complement(1152..2021)
                     /locus_tag="WJK66_000449"
     CDS             complement(1152..2021)
                     /locus_tag="WJK66_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HEZ7578210.1"
                     /translation="MKRIFLPALPAILPLSTYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENNRAELAAPVYIQTGATSFIPIPTEIQENGSNTDMLVGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNGKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDGIRYKSGNYLLLNPNISFAAN
                     DRISLTGGIQWLGTQPDRIDGKKETARNTSTYAHFGTGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            2185..3108
                     /locus_tag="WJK66_000450"
     CDS             2185..3108
                     /locus_tag="WJK66_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ7578211.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGATLDEHTLFEPGKVMFYYRETSSEDEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLVRGEKFFTTQEAEGE
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            complement(3186..3827)
                     /locus_tag="WJK66_000451"
     CDS             complement(3186..3827)
                     /locus_tag="WJK66_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ7578212.1"
                     /translation="MLKPETIHISGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARIPDLLLLIGAAVCHYTGRPEPSAVPNVAKTLMIHG
                     AEDEVVEIGKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(3882..4673)
                     /locus_tag="WJK66_000452"
     CDS             complement(3882..4673)
                     /locus_tag="WJK66_000452"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEZ7578213.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGEKFEIIEADGAEYIKVFRHNTDVILVDGFDGEQIIDALVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            4839..4914
                     /locus_tag="WJK66_000453"
     tRNA            4839..4914
                     /locus_tag="WJK66_000453"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4872..4874,aa:Thr,seq:tgt)
     gene            4949..5024
                     /locus_tag="WJK66_000454"
     tRNA            4949..5024
                     /locus_tag="WJK66_000454"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4982..4984,aa:Thr,seq:tgt)
     gene            5166..5957
                     /gene="panB"
                     /locus_tag="WJK66_000455"
     CDS             5166..5957
                     /gene="panB"
                     /locus_tag="WJK66_000455"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEZ7578214.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVDVLLVGDS
                     LGMAVQGRQSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDDAGAAVVLMECVPAELAKKVTESVSCPTIGIGAGVDCDGQVLVMHDMLG
                     IFPGKTVKFVKNFMQGQSSIQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            6080..6916
                     /locus_tag="WJK66_000456"
     CDS             6080..6916
                     /locus_tag="WJK66_000456"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEZ7578215.1"
                     /translation="MQIIHTIQELRAWRKNAGTVAFVPTMGNLHEGHLALVREAKKRA
                     DNVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLEAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFLGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYRELKAVAESLAQGSLDYA
                     GLEKRAVQSLTEYGWVVDYVEIRRVDTLEVARAGDKKLVVLAAARLGTTRLIDNLEIK
                     LP"
     gene            7081..8919
                     /locus_tag="WJK66_000457"
     CDS             7081..8919
                     /locus_tag="WJK66_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578216.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADAKPPKEVGKVFRKQQR
                     YSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGEAQKRAGWLRNVLRERGNQHLDGLEEV
                     LAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSVQG
                     REKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTGEQRGRAAMTAAMIYADRRDYTKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMFALSKLPDKREALRGLDKIIEKPPAGSNT
                     ELQAEALVQRSVVYDRLGKRKKMISDLERAFRLAPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            9059..9640
                     /locus_tag="WJK66_000458"
     CDS             9059..9640
                     /locus_tag="WJK66_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEZ7578217.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHISSFAAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            9650..10495
                     /gene="ispE"
                     /locus_tag="WJK66_000459"
     CDS             9650..10495
                     /gene="ispE"
                     /locus_tag="WJK66_000459"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEZ7578218.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKSRDDGKIILHNPVDGMPQEADLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQRQLIDSGAALGADVPFFIFGKNAFASGIGEKLI
                     GMDIPKQWYVIVKPPVHVSTAKIFTHEGLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRNSAYNIYRQVSDLYEAYLAEGLSKH
                     PLLSV"
     gene            10503..10578
                     /locus_tag="WJK66_000460"
     tRNA            10503..10578
                     /locus_tag="WJK66_000460"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10536..10538,aa:Gln,seq:ttg)
     gene            10587..10662
                     /locus_tag="WJK66_000461"
     tRNA            10587..10662
                     /locus_tag="WJK66_000461"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10620..10622,aa:Gln,seq:ttg)
     gene            10829..11812
                     /locus_tag="WJK66_000462"
     CDS             10829..11812
                     /locus_tag="WJK66_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEZ7578219.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            11879..12451
                     /locus_tag="WJK66_000463"
     CDS             11879..12451
                     /locus_tag="WJK66_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEZ7578220.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEDQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTSVEVVALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(12883..13101)
                     /locus_tag="WJK66_000464"
     CDS             complement(12883..13101)
                     /locus_tag="WJK66_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578221.1"
                     /translation="MRAVEICGFFFAGDEGAGSGALQVEIGGGFARPIELITFARTGK
                     VFCAEDEFELFKVNLVFTKDFGKELFEF"
     gene            13174..14733
                     /locus_tag="WJK66_000465"
     CDS             13174..14733
                     /locus_tag="WJK66_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ7578222.1"
                     /translation="MIYIDPPYNTGNDSFVYPDKFSESREEYARRVGDTDDAGYLKRD
                     GVFQGAWRKNGKDSGHYHSNWLSMMLPRLHLAKTLLREDGVIFISIDDNEQAQLKLLC
                     DEVFGAENFVGQFPWRKRTAKSDVPFGVSADYEYLLAYARSNAFIASIDAEERRYFET
                     DDFPNRPWRTHDLTTQRNASERPNSFFTMVNPKTGKEYPANPNATWRVTKDTFQEYYN
                     AGKIVFPDDYDFLKIKNPVMRYFKDDDMKKDGERFGRKAVSTRLPENVGMSLNGTKEI
                     TELFKSKLFDFPKPSSLIKYLLQILHVSDGLILDFFSGSGTTAHAVMQLNAEDGGCRR
                     FICVQLPEETDEKSEARKAGFNTIAEIAKERIRRAGRQISDGLQEGAEIDTGFKVFKL
                     AESGFKQWRQPEQSDTEALQGELSLNIDSVLSEMPSENLLYELMLRLGLKLTCKISLS
                     DGVYFVEDEDTGGLYAFLLERVNQDLIDAVLAKHPVKAVVLDRLFDGDDALKGNTVLQ
                     MKDAGVMFECV"
     gene            14968..17619
                     /locus_tag="WJK66_000466"
     CDS             14968..17619
                     /locus_tag="WJK66_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEZ7578223.1"
                     /translation="MELKFEELPYQLDAVNAVANLFAGQPNHARTFDLTSQGTGRFVG
                     NGLDLDWETLGRNLNAVQKQNGQPETEIGTHGLNFSLEMETGTGKTYVYLRTIYELNR
                     RCGWKKFVIVVPSVPIREGVLKSLEITKAHFDGVFGRPVMRYAEYSSTRLSDLRAFAV
                     NDHIEILVINIQSFEKDGNVINKVNESGEAPIKFIQETCPIVIIDEPQNMETEGRLNA
                     LESLNPLFTLRYSATHKKPYHKVYSLNPVQAYNLKLVKQIEVLPVLAENDVNGAFAEL
                     LEVKQAARGRLKADLNIHYQDKKETKKKKVSVKSGDDLFEKSGGNEAYRHGFIIDGMD
                     FENQELAFSSGKTITRGGDGDAVRDEIMKNQIRETVREHLAKEKRLNPSGIKVLSLFF
                     IDRVENYRTEDGTAGKFALWFEEIYRELSGNTDTAGVHDGYFSQDKKGRLKNTDGTTQ
                     ADNDTYRLIMRDKETLLSFGSPLRFIFSHSALKEGWDNPNVFQICMLNETRSPLKKRQ
                     EIGRGLRIAVNQEGRRVRDENVNILTVVPNESYELFAANLQKEYEDECGIKFAAENIK
                     NGNKKKKQLFRKDFPLDPEFQAIWERLKYKTRYRVRFDTEELIKQASAAVAGLPEIQK
                     PKIRIRKAQLKQSQTYGIETVEIASRSRDMETTWEIPDILGEIQKKTGLTRRTVYEII
                     IQSGRLKDVPGNPQRFIDLTAEVVNRSLHALMAAGIEYERTGEVYDQKLLEDMAREGI
                     EFYENNFTFAVGNPQKTICENYIPLDSGTEQAFAQNCEDYDAKNEGGVAFYFKLPSWF
                     KIPTPLGNYNPDWAVVKQVQDKVYFVAETKNTGKSIQAGVDAGKLRENERLKIECGER
                     HFEALGGLAYCVVEKVGDLKLEKEEQA"
     gene            complement(17783..18952)
                     /locus_tag="WJK66_000467"
     CDS             complement(17783..18952)
                     /locus_tag="WJK66_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEZ7578224.1"
                     /translation="MTAHKILPVLLSIILGVSHATAASPAPNKPAVHAAPTFQTPETL
                     TAAHIVIDLQSKQILSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIRSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLALLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILAITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQIYGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQVLGKIKIRQNGYTIAEKEIVALEDVEKKNWWKKLWARLTGQ"
     gene            19100..20626
                     /gene="ilvA"
                     /locus_tag="WJK66_000468"
     CDS             19100..20626
                     /gene="ilvA"
                     /locus_tag="WJK66_000468"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEZ7578225.1"
                     /translation="MNTPLPYSNYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEQEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTDDEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            complement(20683..21756)
                     /locus_tag="WJK66_000469"
     CDS             complement(20683..21756)
                     /locus_tag="WJK66_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEZ7578226.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGKILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKVHAVGALTHILVKHGKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
BASE COUNT         5352 a         5000 c         6027 g         5411 t
ORIGIN      
        1 atccactata tatatccatt catatagaga aaaattctta tacccaatat aaaacaaggt
       61 ctttcattat attttgaaaa acctagaagc tttgtatcta ttggtttatc aaaattaata
      121 attattgcag aagacatgta ggctaataat cttgatttat ctatatttta ttaattatat
      181 atttaacccc gaagtcaata tagaaattta gtgattttaa agttctctta tcaatttcaa
      241 tctcagggag tacataaaaa tcctcagaat aggcaaactc tatatatgca tcatcattcg
      301 ataataattc aataatattt ttcatttcat ccgaaagaaa tgtaaccatc tcatattgac
      361 atttattttt agataccatt tttgaatatt gattaaataa ttttggatta ctatttttag
      421 taattaatcc ttccggagta ataaaaccac tttgtataaa gtcatcactt atattaacag
      481 ccttccgaaa aaaaatataa tcacactctt tgttatcaat ataaaatctc atactcttta
      541 ataatgaaat ataagaattt ccagaattac aagcaatttg aaaaaacatc attactccta
      601 aaactcataa atagtaagga atatatatat atgcatattc cttactattt tttcacaaat
      661 tatgcttatc tatgcttatt tccataaaaa gcatgccctc gaattttttg acttgagttc
      721 tggaaatgag aataatttga atggatttcc ttctgtccat gtctaataac attttgtctt
      781 ccccaagctt ggtttgctac cgctcttgct tgttgagatg ttcgtgcata gacattagct
      841 cctcttgata aagcttgtgc tgcaacacgc tgcgtaaccc aacgctgcgc aaggaatctg
      901 cccgcatgca aaattcctat tgccaccaac ggtgccgcag ccccttcagt ttctttcatc
      961 tccttttgag aaagctccgc caactgcatc ggcgcatctg cctgcgtgtg gaacacttgg
     1021 ttttcaaatg tctgattgtc caagccgttt gccattgcgg gggcaatcat agacagcatc
     1081 attacggctg cggtgatttg ttttttcata ataactcctt tggattacaa ggttggaaaa
     1141 tcaaaacctg cttaaaacgt atgctgtacg ccaaatttca gttcggaact gctttgccct
     1201 gaaacgttga aacgtgcgga tgcgtttaaa gccgtggttt tggtgaaacc gaaacctgtg
     1261 ccgaaatgag cgtaggtgga tgtgtttctt gcggtttctt ttttgccgtc tatgcggtcg
     1321 ggctgtgtgc ccagccattg gatgcctccg gtcaggctga ttctgtcgtt ggcggcaaat
     1381 gagatgttgg ggttgagcag caggtagttg cccgatttgt agcggatgcc gtctgaaagg
     1441 gttttgctgc cgttgatgcg gtaggcggcg gtgagggaaa ggacaatcgg atctatggct
     1501 ttgtaggtgg tggcgccgat gagccaggat tttcccgacg aggctttgtt gcgcgatttt
     1561 tcgtaaaccg tgctttcaag aaagctgatt agggcggggt ttttgtcgtc tttaaggaaa
     1621 gtgtggctga tgccgaggga tacgtcggac atccgtttgt tgcgggtttt gccgttgccg
     1681 tcgagtttgc gttcttcgtg ccacagatag ctgccgctgc cgtaaatgtc ggtattcccg
     1741 gtcagtccgt agcgcaaacc gagcgtgccg acgagcatat cggtattgct gccgttttct
     1801 tggatttcgg tcggaatggg gataaacgag gttgcgccgg tttgaatgta aaccggtgcg
     1861 gcaagttcgg cgcggttgtt ttcgctgttc aggtaggtaa gggaagtttc cagtttccat
     1921 tttcccttgt cggtcattat gtcttcaatc gtcaagggca ggtcggcata agtggataaa
     1981 ggcaggatgg cgggcaaggc gggcaaaaag atgcgcttca tatttctcct attgatatta
     2041 attcttattt actgataata atgaatatcc gataatcttg tctaaataaa tttttaaatc
     2101 ataaaaaaca atatgtttgt ttttgtcaat tatgtttcga tatgccgtct gaaaagtttg
     2161 tgaaaaacgg ttacaatccg ccgcatgaaa aaacgcaata atcctcttcc tctgttgaac
     2221 ggtgtcaaac ccagttattt ggtgctgccg catgaaaagc agttttacgg gctgccgctg
     2281 ctgcattttc tgtgcatccg ctttcctttt gtgggcgcgg acgattggcg caggcggttg
     2341 aacagcggtt ttgtggtcgg ttcggatggt gcgacgttgg acgaacatac tttgttcgag
     2401 ccgggtaagg tgatgtttta ttatcgggaa accagcagtg aggatgagcc gcgtattccg
     2461 tttgaagaaa agattttgca tattgatgag catttgattg tggtggacaa gccgcatttt
     2521 ctgcccgtca tccccagcgg caggtttttg cgggaaaccc tgctcacgcg cctgcgtttg
     2581 cggcctgaat tgcagcattt gaatgttgag gacattacgc cgctgcaccg cttggacaag
     2641 gatacggcag gcgtgatgct gctgtcgcat aatcctgcca cgcgcggagc ctatcagacg
     2701 atgtttcaaa acaaaacggt atggaaaacg tatgaggcgc ttgcgccgac aaggacggat
     2761 ttgccgtatc cgctcgatgt ggtttcgcgt ttggtgaggg gtgagaaatt ttttacgacg
     2821 caagaggcgg aaggcgaacc aaacgcacat acgacggtcg aactgattga aaacaggggg
     2881 gaattcagcc tttaccgcct tacgccgcat acgggcaaga aacaccagtt gcgcgtgcat
     2941 atgatgggtt tgggtatgcc gctgttgaat gacgcgctct atcccgtgcc gtctgaagcg
     3001 ggcagcgagg attatcggaa acctttgaag ctattggcaa aaaagattgc gtttgcagat
     3061 cctttgtcgg gtcgggaaag ggtgttttgc agcggatttt gcctataatg agagggaacg
     3121 caaacccgta tcggcggatg ccgccgatac gggttttaca ttatcgatgc cgtctgaaag
     3181 gggttttatc cgttcaggca tacgggcgcg aagcggagga tggtgtcgcg caggacgatg
     3241 agtttgccgt gaaagaaatg cgtggaaccg gcgatggtaa tgacgggcaa atcttgcggt
     3301 tccgcccatt tcagtgcttt cccgatttcg acgacttcgt cttccgcgcc gtgtatcatc
     3361 agcgttttgg caacattggg aacggcggac ggctcggggc ggccggtata gtggcagact
     3421 gccgcgccga tgagcagcaa taaatcgggt atgcgcgctt gtgcggcaaa tgtggcgaca
     3481 taaccgccga aggagaagcc ggataaggca aattcggggg cttcgggatg ttgggcacgg
     3541 gcatagtcga tgacggcgag gcagtcttgc gtttcgccgc gtccgtaatc gtgtgtgcct
     3601 tcgctgccgc ctacgccgcg aaggttgggt aggtagcagt ggaagccgag tttgcttaag
     3661 gctttggcgg cagtttggat aactttgttg gtgtttgttc cgccctggag ggggttggga
     3721 tgattgatga cggcaacacc gcgtgccggt acttgttcgg acgggatatg gatggtttct
     3781 aaaatgccgg caggaccgga tatgtgtatg gtttcgggtt tcagcataat gatttcctct
     3841 tgattttccg catgttccaa tgcagccgtg aagatagttg cttaaaggtg gaaatgcttg
     3901 ccgttgttgg ggttggacgc tttcaggccg gcaagcatac ggtggaagtc caatccgtat
     3961 gcgttgctca gtttgtcggc acgttttttg agtttgtcta tgttttgctc tttggggctg
     4021 ctttggaagg ccattaccgc gacattgccg tggctttcgg caggaagttc caagacgcgc
     4081 ccttcaaaaa cgctcaacaa ccgttcgatg aagcgttggt agcgtttgtc gccgctccac
     4141 cagttggtta cgaatatgcc gtctgaagag agtgcgtttc ggcagtctcg gaaaaacggt
     4201 tcttcaacca gcgcatcgat gatttgttcg ccgtcgaacc cgtccaccaa aatcacatcg
     4261 gtgttgtggc ggaagacttt gatatattct gcaccgtctg cttcaataat ttcaaatttc
     4321 tcgccctcga aaggcaactc aaacaggttg cgggcaatgg cgatgacctg aggattgatg
     4381 tccacggcgg tttggcgcgt gtcgggcagg taggtgtcta tccagcgtgc aaacgagccg
     4441 ccgcccaagc cgatttgggt gatatgctgc ggaagtgcgt cggtaaacag cagccagccc
     4501 atcatcgcgc ggctgtaaga gagtaccagc tcggacgggt ggtcgaggtt cattgagctt
     4561 tgaacggtgt cgctgcccaa gtgaagcgaa cggatattgc cttcttcgga aatgccgact
     4621 tcgggaaagc cggattttgc aggacgcagg cggcggtagg gatgtcttgc catcggtgtg
     4681 aacggtcggt ttgaaaggcg gatattttat cagaatgccg atgcttgtcc tgcttcaaat
     4741 taatttcttt tcaaataaat tacttattcg gatttgccgg ggctttcgga taaattcctt
     4801 gccaaagtgt gaagttgcct gcataattcg ccttctttgc cggtatagct cagttggtag
     4861 agcacctgac ttgtaatcag ggggtcccga gttcgactct tggtgccggc accatttctg
     4921 ctgatggtgt tgcaaaacat ttcagtaagc cggtatagct cagttggtag agcacctgac
     4981 ttgtaatcag ggggtcccga gttcgactct tggtgccggc accagatttg acagtccgat
     5041 tgtgtaaaac agtcgggctg ttttgcattt gcggcacggt cggtggcagg gctgcgggca
     5101 tttcactata atagcccgtt tcaaactgaa aaggccgtct gaaaagggcg gggtaacaat
     5161 atctgatgat tactgtgaac acactgcaaa aaatgaaggc ggcgggcgag aaaatcgcta
     5221 tgctgaccgc ttacgaatcc agttttgccg cgctgatgga cgatgccggc gtggatgtgc
     5281 tgctggttgg ggattctttg ggaatggcgg ttcaagggcg gcagtcgacg ctgcccgtca
     5341 gcctgcgcga tatgtgttat cacaccgaat gtgtagcacg cggtgcaaaa aatgcgatga
     5401 ttgtcagcga tttgccgttt ggtgcatatc agcagagtaa ggagcaggcg tttgccgccg
     5461 ccgccgaact gatggctgcc ggcgcgcata tggtcaaact cgaaggcggt gtgtggatgg
     5521 cggaaacgac cgaatttctg caaatgcgcg gtattccggt ctgtgcccac atcggtctga
     5581 ccccgcaatc cgtgtttgcc ttcggcggat acaaggttca ggggcgcggc ggcaaggcgc
     5641 aggcgttgct taacgatgcc aaggcgcatg atgatgcggg ggcggcggtc gtgctgatgg
     5701 agtgtgttcc tgcggaactg gcaaaaaagg taactgaatc cgtttcctgt ccgaccatcg
     5761 gcatcggcgc gggtgtcgat tgcgacgggc aggttttggt gatgcacgat atgctcggca
     5821 ttttcccggg taagacggtg aaattcgtca aaaactttat gcaggggcaa agcagcattc
     5881 aggcggcggt tcgggcgtat gttgccgaag tcaaagccaa aaccttccct gctgcggaac
     5941 atatttttgc agactgaaca aggtttgctg ccgatgccgt ctgaaagccg tttcagacgg
     6001 cattttgttt tgccgtgcgc ggcgcgtata atcggcgcgt tttgtcgggc aggaagcccg
     6061 aaggataagg attaccgtta tgcaaatcat acataccatt caagaactgc gcgcgtggcg
     6121 taaaaatgcg ggaacggtgg catttgtgcc gaccatgggc aatctgcacg aagggcatct
     6181 tgccctcgtt cgcgaagcca aaaaacgcgc cgacaacgtt gttgtcagca tattcgtcaa
     6241 ccgcctgcaa ttcggtcagg gcgaggattt cgacaaatat ccgcgtactt tgcaacagga
     6301 tgcggacaag ctggaagccg agggtgttgc cgttgttttc gcgcccgatg agaaagaact
     6361 ctatccgaac gtggaacagc gttacaacgt cgaaccgccc aatctgcaaa atgagttgtg
     6421 cggcaagttt cgtccgggac atttcctcgg ggtggcgacg gtggtgtcca agctgttcaa
     6481 tattgtcgcg ccggatgttg cctgtttcgg caaaaaagac tatcagcagc ttgccgtgat
     6541 taaaggtttg accgaggatt tgaattttga tgttgaaata gtgcctgtcg atacggggcg
     6601 cgcggaagac ggtttggcac tctccagccg caaccagtat ttgagtgcgg cggaacgcga
     6661 cgaagcaccg cgcctgtacc gcgaattaaa ggctgttgcc gaatccttgg cgcagggcag
     6721 tttggattat gcaggtttgg aaaaacgtgc cgtccaatcc ctgacagaat acggctgggt
     6781 ggtcgattat gtcgaaatcc gccgcgtcga tacgctcgaa gtggcgcggg cgggagataa
     6841 gaaactggtg gtcttggccg ccgcccgtct ggggacgacg cgcctgattg acaatttgga
     6901 aataaaactc ccttaaaccg caagcgtcgg gaatgccgtc tgaagcggat ttgcgtttca
     6961 gacggcattt atttttttga acggggtttc gcaaatctac aaacgattct gcttgtgata
     7021 aagttacgcc tgattatatg ccgtctgaaa gttcggacgg ctgtcggata aaggaagatt
     7081 atgttacccg cccgtttcac cattttatct gtgctcgcgg cagccctgct tgccgggcag
     7141 gcgtatgccg ccggcgcggc ggatgcgaag ccgccgaagg aagtcggaaa ggttttcaga
     7201 aagcagcagc gttacagcga ggaagaaatc aaaaacgaac gcgcacggct tgcggcagtg
     7261 ggcgagcggg ttaatcagat atttacgttg ctgggagggg aaaccgcctt gcaaaagggg
     7321 caggcgggaa cggctctggc aacctatatg ctgatgttgg aacgcacaaa atcccccgaa
     7381 gtcgccgaac gcgccttgga aatggccgtg tcgctgaacg cgtttgaaca ggcggaaatg
     7441 atttatcaga aatggcggca gattgagcct ataccgggtg aggcgcaaaa acgggcgggg
     7501 tggctgcgga acgtgctgag ggaaagagga aatcagcatc tggacggact ggaagaagtg
     7561 ctggctcagg cggacgaagg acagaaccgc agggtgtttt tattgttggc acaagccgcc
     7621 gtgcaacagg acgggttggc gcaaaaagca tcgaaagcgg ttcgccgcgc ggcgttgaaa
     7681 tatgaacatc tgcccgaagc ggcggttgcc gatgtggtgt tcagcgtaca gggacgcgaa
     7741 aaggaaaagg caatcggagc tttgcagcgt ttggcgaagc tcgatacgga aatattgccc
     7801 cccactttaa tgacgttgcg tctgactgca cgcaaatatc ccgaaatact cgacggcttt
     7861 ttcgagcaga cagacaccca aaacctttcg gccgtctggc aggaaatgga aattatgaat
     7921 ctggtttccc tgcacaggtt ggatgatgcc tatgcgcgtt tgaacgtgct gttggaacgc
     7981 aatccgaatg cagacctgta tattcaggca gcgatattgg cggcaaaccg aaaagaaggt
     8041 gcttccgtta tcgacggcta cgccgaaaag gcatacggca gggggacggg ggaacagcgg
     8101 ggcagggcgg caatgacggc ggcgatgata tatgccgaca gaagggatta caccaaagtc
     8161 aggcagtggc tgaaaaaagt gtccgcgccg gaatacctgt tcgacaaagg tgtgctggcg
     8221 gctgcggcgg ctgtcgagtt ggacggcggc agggcggctt tgcggcagat cggcagggtg
     8281 cggaaacttc ccgaacagca ggggcggtat tttacggcag acaatttgtc caaaatacag
     8341 atgttcgccc tgtcgaagct gcccgataaa cgggaggctt tgagggggtt ggacaagatt
     8401 atcgaaaaac cgcctgccgg cagtaataca gagttacagg cagaggcatt ggtacagcgg
     8461 tcagttgttt acgatcggct tggcaagcgg aaaaaaatga tttcagatct tgaaagggcg
     8521 ttcaggcttg cacccgataa cgctcagatt atgaataatc tgggctacag cctgctttcc
     8581 gattccaaac gtttggacga aggcttcgcc ctgcttcaga cggcatacca aatcaacccg
     8641 gacgataccg ctgtcaacga cagcataggc tgggcgtatt acctgaaagg cgacgcggaa
     8701 agcgcgctgc cgtatctgcg gtattcgttt gaaaacgacc ccgagcccga agttgccgcc
     8761 catttgggcg aagtgttgtg ggcattgggc gaacgcgatc aggcggttga cgtatggacg
     8821 caggcggcac accttacggg agacaagaaa atatggcggg aaacgctcaa acgtcacggc
     8881 atcgcattgc cccaaccttc ccgcaaaccc cggaaataat gcaggtccat cctttcagac
     8941 ggcataaggt ttgccgggaa gctgcggcat tcgggcaaac ggcacgcagt tcgcacgcgt
     9001 tttgcacggc acgccgaacc cgccggccgg caggatggca cccgttaagg aaattctgat
     9061 gaaacacacc gtatccgcat cggtcatcct gcttttgacc gcttgcgcgc aattacctca
     9121 aaataacgaa aacctgtggc agccgtccga acacatcagc agttttgcag cagaagggcg
     9181 gttggcagtg aaagcggaag ggaaaggttc gtatgcaaat ttcgattgga cataccaacc
     9241 gcccgtggaa accatcaata tcaatacccc tttgggcagt acgctcgggc agttgtgtca
     9301 agacagggac ggcgcattgg cagtggacgg caaaggaaat gtctatcagg cggaaagtgc
     9361 ggaagaattg agcaggcagc tggtcggttt caaactgcca atccaatatc tgcatatctg
     9421 ggcagatggc aggcgtgtgg cgggcgcgcc ttaccgcatc ctgccggacg gcatattgga
     9481 acaatacggt tggactgtcg gcagaaccgc cgacagtggg gggcaagtcc gaacgttgca
     9541 actgaataac ggaaatttga acatcaggct ggttttcacc gaaatcggta tgccgtctga
     9601 aactgaaacc ccggaacgct gtgcggcgcg cacgagataa ggcggacaga tgaatattgc
     9661 ggacggacgg caggcgtttc ccgcacctgc aaaactgaat ctcgatttga ggattaccgg
     9721 caggcgggaa gacggttatc acaatatcga aagcatattc tgcctgatag atttgcagga
     9781 taccgtatat ttgaaatcga gggatgacgg caaaatcatc ctgcacaatc cggttgatgg
     9841 aatgccgcag gaagcggatt taagctaccg tgccgcatcg ttgctgcaaa aatatgcgcg
     9901 caaccccgcc ggcgtggaaa tatggctgga caaaaaaatc ccgacagggg cgggtttggg
     9961 cggcggaagc tcggatgcgg caaccgtttt gctggtgttg aaccgttggt ggcagtgcgg
    10021 tctgacgcag cggcagctca ttgattcggg cgcggctcta ggggcggacg taccattttt
    10081 tattttcgga aaaaatgctt ttgcaagcgg gataggtgaa aaactgatag gaatggatat
    10141 tccgaaacag tggtatgtca tcgtcaaacc gcccgtccac gtttccactg caaaaatttt
    10201 cacacacgaa ggcttgacac gaaattccgc ctcaagcata atgccgactt tccaaaacct
    10261 gcaaccgttt agaaatgaca tgcaggcagt tgtatttaaa gaataccctg aagtttggaa
    10321 agcctattcc gaattgtccc gatatggatt tgccttaatg acaggttctg gtgcgtgtgt
    10381 attcacggcg tgtcaagata ggaatagcgc atacaatata taccgacaag tttcagattt
    10441 gtacgaggca tatttggcag agggtctttc aaaacatcct ttgttgtccg tataaacatt
    10501 gttggggagt cgtcaagcgg ttaagacact ggattttgat tccagcatgc gaaggttcga
    10561 atccttcctc cccagccagc cctttttggg gagtcgtcaa gcggttaaga cactggattt
    10621 tgattccagc atgcgaaggt tcgaatcctt cctccccagc caaataaaag cgtgtaagcc
    10681 tgcttacacg cttttatttc atagaaataa aaatattgaa atgcctttgt ttgtgtcgga
    10741 tgttgcaggt ataatgtcgg gcttggtaca agcagaggga agcattgtgt tttctgagcg
    10801 gaagttaaac ataaaatcag gtgagaatat ggctgcgtac gacagtttga tggtatttac
    10861 aggcaatgcc aatcccgaat tggcacaacg tgttgtcagg catttggaca tttctttggg
    10921 caatgcttcc gtatccaagt tttcagacgg cgaagttgcc gtcgaactgt tggaaaacgt
    10981 acgcgggcgc gatgttttca tccttcagcc gacctgtgcg ccgaccaatg acaacctgat
    11041 ggaaatcctg acgatggcgg atgcactgaa gcgtgcttcg gcaggtcgta ttaccacagc
    11101 cattccgtat ttcggctatg cgcgccaaga ccgccgtccg cgttccgtcc gcgttccgat
    11161 ttctgccaaa ctggtggcaa atatgctgta ttcggcaggg atcgaccgtg ttttgactgt
    11221 cgatttgcat gccgaccaga ttcaaggttt cttcgatatt ccggtggaca atatttatgc
    11281 caccccgatt ctgttgaacg acatcaaaca acagcggatt gaaaatctga ccgtcgtcag
    11341 cccggacatc ggcggtgtcg tccgcgcccg cgccgtggca aaatccctga atgccgactt
    11401 ggcaatcatc gacaaacgcc gcccgaaagc caatgtggcg gaagtcatga acatcatcgg
    11461 cgatattcaa ggcagaacct gtctgattgt ggacgatatg attgacaccg caaatacgct
    11521 gtgcaaagcc gccgtcgctc tgaaagagcg gggggcggaa cgtgtcttgg catatgccag
    11581 ccacgccgta ttctccggag aggcagtcag ccgtattgcc tcatccgaaa tcgaccaggt
    11641 ggtcgtaacc gataccattc ctttgtctga agcggctaaa aactgcgacc gtatccgtca
    11701 ggtaacgatt gccggtctgt tggccgaaac cgtccgccgc attagcaatg aagaatccgt
    11761 ctcatatctt ttcaatgaag aagtgatgac aggcagcatg ttgctgccat aagcccgaag
    11821 ccgtcttaag ctggtcgcgg ccgatgacgg cgattttacc taacttggag tatttaacat
    11881 gacttatgaa attcaagctt ctgttcgtga agcacaaggc actggtgcga gccgccgcct
    11941 gcgtcgcgaa ggccaaatcc ccggcattct gtacggtgaa gaccaagagc ctgttgcaat
    12001 cgctgtggat cacaaaaccg tattctacgc attggaaaaa gaatctttcc atactgcgtt
    12061 gattaagttg tctctgaacg gtgaaaccaa agacgttatc gtccgcgatt tccaaatgca
    12121 cccgttccgc cgcgaagttc aacacatcga cttccaagct gtgaaagccg atcaacttgt
    12181 acgcatccgt gttcccctgc acatcgttaa cgctgaaaat tcccaagcgg tcaaactgca
    12241 aggcggccgc gtatctctgt taaacacttc tgttgaagta gttgctttgc ctgccaacat
    12301 ccctgctttc ttggatttgg attgtgccga agtggttgcc ggcgacattc tgcacttgtc
    12361 agacatcaaa ctgcctgaag gtgtagaaag cgtttccctg aaacgtaacg aaaatctggc
    12421 tgttgctacc gttaccggta agaaacgcta attgattgaa aagcaggcgg tacgtatatg
    12481 atgcgtaccg ccttattgtt tttggctgtg tttcaatatt tggttgattt catccgttta
    12541 taagggaaaa ccgcttattt ccgttcgggt ggaaatttat ccagacgtat tgcgcgacaa
    12601 cggatggttg gctcaaaaac ggcatttcgt accggatgaa aatgaagaag atatgtcgtt
    12661 cacattccgg tagctgtccg gctcgaaccg gctatcagag atagtcggtc aattttcacc
    12721 ctgaaaatca cttttctttt aacttgcaac atattccgat aaaatggaca ctttgtaagt
    12781 aatcattgat ggaagacgca attatggcaa ccgaaccgaa ccgaaccgaa ccgaaccgaa
    12841 attatacacg atttagaaga taatttcaaa caaaacaata tattaaaact caaacaactc
    12901 tttcccgaag tcttttgtga agaccaaatt gactttgaaa agctcaaact cgtcctcggc
    12961 gcagaaaacc ttgccggtgc gggcgaacgt tatcaactcg attgggcggg caaaaccgcc
    13021 tcctatctca acctgcaaag ccccacttcc cgcaccctcg tcccctgcaa agaagaatcc
    13081 gcagatttcg acggcacgca aaacgtgttt atcgaagccg aaaacctcga agcactgaaa
    13141 atcctgcaaa aatcctacgc cggcagcgta aagatgattt acatcgaccc gccctacaat
    13201 accggcaacg attccttcgt ctatcccgac aaattctccg aaagccgcga agaatacgcc
    13261 cgccgcgtgg gcgataccga cgacgcgggt tacctgaagc gcgacggcgt gttccagggc
    13321 gcgtggcgca aaaacggcaa agacagtggc cattaccaca gcaactggct ctctatgatg
    13381 ctgccgcgcc tgcatctggc gaaaaccctg ctgcgcgaag acggcgtgat ttttatcagc
    13441 attgacgaca acgaacaggc gcagttgaaa ttgttatgtg atgaagtgtt tggggcggag
    13501 aattttgtcg gtcagtttcc ttggcgcaaa cgcacggcaa aatcggacgt gccgttcggc
    13561 gtttccgccg attatgaata tctgcttgcc tatgcccgca gtaatgcctt tattgccagt
    13621 atcgatgcgg aagaacgcag atattttgaa accgatgatt tcccgaatcg cccgtggcgg
    13681 acgcatgatt tgactactca aagaaatgcc agtgaacgac caaatagttt tttcactatg
    13741 gttaatccta aaacaggcaa agaatatccg gcgaatccaa atgcaacttg gcgtgttact
    13801 aaggatacat ttcaagaata ttacaatgcg ggaaaaatcg ttttccccga tgattacgat
    13861 ttcctcaaaa ttaaaaaccc cgttatgcgt tattttaaag atgacgacat gaagaaagac
    13921 ggagagcgct tcggacgtaa ggcagtcagt acacggctac ctgaaaatgt aggtatgtcg
    13981 ctgaacggta caaaagaaat taccgaactg tttaaaagca agttgtttga ttttcccaaa
    14041 ccgtcatccc taattaaata tttactgcaa atcctccatg tttcagacgg cctcatccta
    14101 gacttcttct ccggcagcgg cactaccgcc catgccgtga tgcagcttaa cgccgaagac
    14161 ggcggctgcc gccgtttcat ctgtgtccag cttcccgaag aaaccgacga aaaatccgaa
    14221 gcgcgcaagg cgggtttcaa cacgattgcc gaaattgcca aagaacgcat ccgccgcgcg
    14281 ggcaggcaga tttcagacgg ccttcaagag ggagcggaaa tagatacggg ttttaaagtt
    14341 ttcaaacttg ctgaaagcgg tttcaaacaa tggcggcagc ccgagcagtc cgatacggaa
    14401 gccctgcaag gcgagttgtc cttgaacatt gattccgtgt tgtctgaaat gccgtctgaa
    14461 aacctgctct acgaattgat gttgcgttta ggtttgaagc tgacttgcaa gatttcgctt
    14521 tcagacggcg tgtatttcgt ggaagacgag gatacgggcg gactgtacgc cttcctgctg
    14581 gaacgcgtga atcaggattt gattgatgcc gtgctggcga agcatcccgt caaggcggtg
    14641 gttttggaca ggctgttcga cggcgacgac gcgctcaaag gcaataccgt attgcagatg
    14701 aaggacgcgg gcgtgatgtt cgaatgcgtg tagtcagccg aaagcgggaa gtgccgtctg
    14761 ccgtcattcc cgcgcaggcg ggaatctaga aactcaaagt tgcaagaatt tatcggaaat
    14821 gactgaaacc caacgaacct agattcccac tttcgtggga atgacgatat atcagtttct
    14881 gcgccgacag gtctggattc ccgcctgcgc gggaatgacg gcaaagaggt agattttccg
    14941 cgcgacagcg gatagaagga aataaaaatg gaactgaaat ttgaagaact gccctatcag
    15001 cttgatgcgg taaatgccgt tgcgaatctt tttgcagggc agccgaacca cgcccggaca
    15061 tttgatttga ccagtcaggg gacggggcgg tttgtcggca acggcttgga tttggattgg
    15121 gaaacgctgg gtcggaatct gaatgcggtg caaaagcaaa acggacagcc tgaaacggaa
    15181 atcggcacac acgggctgaa tttttctctg gaaatggaaa cgggtacggg caaaacctat
    15241 gtctatctgc gcacgattta cgaattgaac cgccgttgcg gctggaagaa atttgtgatt
    15301 gtcgtgccga gtgtgccgat acgcgaaggc gtgttgaaaa gcctggaaat tacgaaggcg
    15361 cattttgacg gagtgttcgg caggcccgtg atgcgttatg cggaatattc aagcacgcgg
    15421 ctgtcggatt tacgcgcttt tgcggtaaac gaccatatcg aaattttggt aatcaatatc
    15481 cagtcgtttg aaaaagacgg aaacgtgatc aataaggtaa acgaaagcgg ggaagcaccg
    15541 attaagttta ttcaggaaac gtgtcctatc gtcattatcg acgagccgca gaatatggaa
    15601 acggaaggcc gtctgaatgc cttggaatcg ctcaatccgc tctttaccct gcgctattcg
    15661 gcaacgcata aaaaaccgta tcacaaggtg tacagcctga atcccgtgca ggcatacaat
    15721 ttgaagctgg tcaagcaaat cgaagttttg ccggtattgg cggaaaacga tgtgaacggc
    15781 gcatttgcgg agctgctgga agtgaaacag gcggccagag gccgtctgaa agccgatttg
    15841 aatattcatt atcaggataa aaaggaaacc aagaagaaaa aggtatccgt caaatcggga
    15901 gacgatttgt ttgaaaaatc gggcggcaac gaagcctacc gccacggttt cattatcgac
    15961 ggcatggatt tcgagaatca ggaattggcg ttctccagcg gtaaaaccat tacgcgcggc
    16021 ggagacggcg atgcggtgcg cgatgaaatt atgaaaaacc aaatccgcga aacggtaagg
    16081 gagcatttgg cgaaggagaa acggctgaat ccgtcgggga ttaaggtgtt gtcgctgttc
    16141 ttcattgacc gcgtggagaa ttaccgcaca gaagacggaa cggcaggcaa gtttgccctt
    16201 tggtttgaag aaatttatcg ggagctttca ggcaataccg atacggcagg cgtacacgac
    16261 ggctatttct ctcaagacaa gaaaggccgt ctgaaaaaca ccgacggcac gacgcaggcg
    16321 gacaacgata cctaccgcct cattatgcgc gacaaggaaa ccctgctttc cttcggcagc
    16381 ccgctgcgct ttatcttttc ccattccgcg ctgaaagaag gctgggacaa ccccaacgtt
    16441 ttccaaatct gcatgctcaa cgaaacccgc tcgccgttga aaaagcggca ggaaatcggg
    16501 cgcgggctgc gtatcgccgt caatcaggaa ggacggcgcg tgcgcgatga aaacgtgaac
    16561 atactgaccg ttgtgccgaa cgaaagctac gaattgtttg ccgcaaatct gcaaaaggaa
    16621 tacgaagacg aatgcggtat caaatttgcc gccgaaaaca tcaaaaacgg caataagaag
    16681 aaaaaacagc tattccgcaa agattttccg ctcgatcctg agtttcaggc aatttgggag
    16741 aggttgaaat acaaaacgcg ctaccgtgtg cgcttcgata cggaagaatt gattaaacag
    16801 gcttcggcgg cagtagcagg cctgcccgaa atccagaagc cgaaaatccg catccgtaag
    16861 gcgcaattga aacaatctca aacctacggt atcgaaacag tcgaaattgc cagccgcagc
    16921 cgcgatatgg aaaccacatg ggagattccc gacatactcg gcgagatcca aaaaaagacg
    16981 gggctgacgc ggcgcacggt gtacgaaatc atcatacagt caggccgtct gaaagatgtg
    17041 ccgggcaacc cgcaacgctt catcgacctt accgccgaag tggtaaaccg cagcctgcac
    17101 gcgttgatgg cggcaggaat cgagtatgag cgaaccggcg aagtgtacga ccaaaagctg
    17161 ttggaagata tggcgcgcga aggcatcgag ttttatgaaa acaactttac gtttgcagtc
    17221 ggtaatccgc aaaaaaccat ttgcgaaaac tatatccctc tggattccgg cacggaacaa
    17281 gcctttgccc aaaattgcga ggattatgat gcgaaaaatg aaggcggcgt ggcgttttac
    17341 ttcaaactgc ccagctggtt caaaatccca accccgctgg gcaactacaa tcccgattgg
    17401 gcggtggtga aacaggttca ggacaaagtg tatttcgttg cagaaaccaa aaataccggc
    17461 aaaagcattc aggcgggggt agatgcgggc aagctgcgcg aaaacgaacg gctgaaaatc
    17521 gaatgcggtg aacggcattt tgaagcactg ggcggtttgg catactgtgt tgtggaaaaa
    17581 gtgggggact taaagttaga aaaagaggag caggcttaaa acgaacaggt gtaatcatag
    17641 tatggtgatg tagtgcttgg ggcgggcggc aactgaagat gcggatgcgg caattacctt
    17701 gattcactaa accgattggg ggttgaagat tgtttcagac ggcattttaa ttctcctatc
    17761 ttgggcatgg aaatatagaa gcttactgtc ccgtcaggcg tgcccagagt tttttccacc
    17821 agtttttctt ttctacgtcc tccagtgcga caatttcttt ttcggcaatg gtgtatccgt
    17881 tttgtctgat tttgattttt cctaggacct gccctttttt taccggggcg ggaatcggct
    17941 gtatggtttc caaaatctgt tctgccattt tcgcttcctt atgtggcaga gtgatgtagg
    18001 cttctttgag gaagcctgcg cggacggttt ttttgctgcc tccgtaaatt tggatttggg
    18061 caacggtttt tcctttcgga tatattttgg gcgtatcgaa ggcctgcaat gcccagttca
    18121 gcagcttgct gttgtccgat gcgcgtgttt ccgccgattc cgaacccaat gtgatggcaa
    18181 ggatgtgcct gccgttgccg gagtatgaca cggcaaggtt gtagccgccg ctttctgtgt
    18241 gtccggcttt cagaccgttt acattgttgt ccctatataa aaggatattg cggttgtttt
    18301 gttctatatt tttgaatttg aaagatttga tggaaaacag cgggtaatat tccggaaagt
    18361 cgcgcatcaa tgcttcagac agcagggcga ggtctttggc ggtggaaacc tgtccttctc
    18421 tactcaagcc tgtcgggttt ttgaatacag tgttcttcat gcccaagcgt cgggcttctt
    18481 tgttcatttg ttgcacaaaa ttttcaatcg agccgttgcc cagccggccg gcaagggtta
    18541 gggcggcatc gtttgcggat agtgcaatca tgccttttaa gagtttgtcg gtgctgaccg
    18601 tatcgccggg acgtacaaac attctgcttc cttctgaagc ccatgcggat tcgggtattt
    18661 ttaagttttc ttcagatcgg atatttcccg atttcatgtt tttgaaaacc agatatgcgg
    18721 tcatcagttg ggttagtgcc gccggttcga caggggtatt gatgtttttg gcggataaaa
    18781 tctgtttgct ttgaaggtcg ataacgatgt gtgccgctgt gagggtttcg ggtgtttgga
    18841 acgtgggggc ggcgtgtacc gccggtttgt tgggcgcggg cgatgcggcc gttgcgtgag
    18901 aaacgcctaa gatgatggaa agcaggacgg gcaggatttt atgtgctgtc atgaaatatt
    18961 ctaattgtgt gcgtgtttca gtctgccgat tatacgctta gggtgtctga tcgggcggat
    19021 ttttcttgct ttcgcgccgt cttgggcgta tggttttggg ttttgcgatt ttaataaacc
    19081 gattatccca tattgaatta tgaacacgcc ccttccttat tccaattacc tcatccgcat
    19141 cctgacggca tctgtctatg atgtcgcggt cgaaacgcct ttggaaccgg cacgcagcct
    19201 ttctgtacgt ttgaaaaaca acatcctttt gaaacgcgaa gatttgcagc cggttttttc
    19261 gttcaaaata cgcggcgcgt acaacaaaat gtccaagttg ccgaaagatg cgctcgcttg
    19321 cggcgtgatt gcggcaagcg cgggcaatca cgcacaaggc gtggcattgt ccgcacagcg
    19381 tttgggttgc cgtgccgtta tcgtcatgcc ggagactacg ccgaaaatca aagtggatgc
    19441 ggtcaaaagc cgtggcggcg aggtggtttt gcgcggtgtt tcatacaacg atgcttacga
    19501 ttatgcgatg gagctggcgg aacaggaagg gctgacctat atcgcaccgt ttgacgatcc
    19561 tgatgtgatt gcaggacaag ggacggtagg gatggaaatt gtcagccagc atcccgatcc
    19621 aatccgcgcc gtattcgtac cgataggcgg tggcggtttg gcggcgggcg tggcggcatt
    19681 tatcaagcag gtccgtcccg aaatcaaagt tatcggcgtt cagaccaacg attcctgctg
    19741 tatgaagcag tcggtcgaag cgggtgaaat cgtccatttg aaagatgtcg ggctgttttc
    19801 agacggcacg gcggtcaaag tcgtcggaaa cgaaaccttc cgcctctgca aagaactttt
    19861 ggatgaaatc attacagtcg ataccgatgc ggtttgcggc gcggtcaagg atattttcga
    19921 tgacacgcgc agcattaccg agccggcggg cgcgttggcg ttggcgggtc tgaaagccta
    19981 tatcgcccga gaaggcgcgg aaaaccaaac cctgattgcc gttaccagcg gtgcgaatat
    20041 gaattttcac cgtttgcgcc acgtttcgga acggagcgaa ttgggcgagg gcaacgaagg
    20101 tatttttgcc gttaccatcc ctgaagaacg cggcagcttc cttaagtttg tcaatatatt
    20161 gggaaatagg aatattaccg agttcaacta ccgctacggg gacgatgaaa aagcgcatat
    20221 ctttgtcgga cttcaagcgg caggcccgca ggatttggcg gttatcggca gccggttgga
    20281 tgaggcggga ttgcccaatg tcgacctgac cgatgatgag attgccaaaa tccatatccg
    20341 ctatatggtc ggagggcgga cggacaaagt agaaaacgag cgtctggtca gttttgagtt
    20401 tccggagcgt ccgggggcgt tggcgcgttt tttgaaccat atgcagggcg gttggaacat
    20461 tacgctcttc cattaccgca accacggtgc ggattacggg cggattttgg tcggtatcga
    20521 tgtgccgccg cacgatgccg ccgcatttga cggtttcttg gaaagtctgg gatacagcta
    20581 tcacgaggaa acgcaaaatg ccgcgtacaa gctgtttctt gcctgacgct tgaaagcaca
    20641 atgccgtctg aaagcctttc agacggcatt gcgctttcat ggttaaatcg aatattcaat
    20701 cagttcgttt tgagagaaga cataaacctg tttcggaatc agcttcaatt ctttgccttc
    20761 ggcgattggg taacgcgcgg catcgctgcc agccagcgtg atatgcacgt cttgtttgcc
    20821 gtgtttcacc aaaatatgcg tcaatgcccc gacggcgtgg actttttcga tttcggcaca
    20881 aatcatcggt gtttcgtgtt cggcggcgat ctgccattcg tgcgggcgga tatagccggt
    20941 ggcggtttgt tcctgccatt tgtattgtgc gtccaatttc cacgcgaagc cgttataatg
    21001 ccagaagcct ttttcgatgc gtccttcaaa agcgtcggtt tcgccgagga actcggtaac
    21061 gaaggcattt tcgggtttgc ggtaaatagc ttcggcgctg ccggtttgtt cgattttgcc
    21121 gtggttcatc acgacgattt cgtcggaaac ttcgagggct tcttcttggt cgtgcgtaac
    21181 cagaatgctg gttacaccca ggttgtgatg gatgtcgcgc agccaggtgc gtaattcttt
    21241 gcgtactttg gcatccaacg cgccgaaggg ttcgtccaaa agcaggagtt tcggttcgac
    21301 cgcaagcgca cgggcgaggg cgatgcgctg gcgttgcccg ccggagagtt ggtgcggata
    21361 ggattttgcc aaatgcgaga gctgcacgag cttgagcaat tcttcgactt tggcgcggat
    21421 ttgtcctttg gacgggcgtt cggacttggg caatacggtc aaaccgaagg caacgttgtc
    21481 aaacacgttc atatggcgga aaagggcgta gtgttggaac acgaagccga ctttgcgctc
    21541 gcgcacgtgc ttggcggtta cgtcttgccc atcaaacaga atcttgccgc cgtcggcgtt
    21601 ttccagtccg gcgataatgc gtaaaagcgt ggttttgccg cagccggacg ggccgagcag
    21661 ggaaacgagt ttgccggtgg ggacgttgag gttgatgttt ttcagcgcgt gaaaattgcc
    21721 gaagtgtttg tttaagtttt ggatggtgat actcatattg cattcctttc ggcggcggcg
    21781 agttttttgt
//
LOCUS       DAWKOG010000016        21637 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.016, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000016 DAWKOG010000000
VERSION     DAWKOG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21637
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.016"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(66..1247)
                     /gene="odhB"
                     /locus_tag="WJK66_000470"
     CDS             complement(66..1247)
                     /gene="odhB"
                     /locus_tag="WJK66_000470"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEZ7578227.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPAAAPAEAAPAAAPAA
                     AQNNAAMPAAAKLAAETGVDVNALQGSGRDGRVLKEDVQNAAAKPAAAAAPAVTLPAG
                     ARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGV
                     KLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDAD
                     QMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAIL
                     GMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPARLLLD
                     L"
     gene            complement(1347..4175)
                     /locus_tag="WJK66_000471"
     CDS             complement(1347..4175)
                     /locus_tag="WJK66_000471"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEZ7578228.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(4380..5663)
                     /gene="gltA"
                     /locus_tag="WJK66_000472"
     CDS             complement(4380..5663)
                     /gene="gltA"
                     /locus_tag="WJK66_000472"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ7578229.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPPGER"
     gene            complement(5775..6023)
                     /locus_tag="WJK66_000473"
     CDS             complement(5775..6023)
                     /locus_tag="WJK66_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEZ7578230.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            complement(6027..6734)
                     /locus_tag="WJK66_000474"
     CDS             complement(6027..6734)
                     /locus_tag="WJK66_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEZ7578231.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            complement(6855..8618)
                     /gene="sdhA"
                     /locus_tag="WJK66_000475"
     CDS             complement(6855..8618)
                     /gene="sdhA"
                     /locus_tag="WJK66_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEZ7578232.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPANAGELTRQRIERLDSQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(8621..8962)
                     /gene="sdhD"
                     /locus_tag="WJK66_000476"
     CDS             complement(8621..8962)
                     /gene="sdhD"
                     /locus_tag="WJK66_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEZ7578233.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSIKVIWG"
     gene            complement(8956..9333)
                     /gene="sdhC"
                     /locus_tag="WJK66_000477"
     CDS             complement(8956..9333)
                     /gene="sdhC"
                     /locus_tag="WJK66_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEZ7578234.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(9612..11015)
                     /locus_tag="WJK66_000478"
     CDS             complement(9612..11015)
                     /locus_tag="WJK66_000478"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ7578235.1"
                     /translation="MKKIQADVVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMQRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHTVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(11264..12001)
                     /locus_tag="WJK66_000479"
     CDS             complement(11264..12001)
                     /locus_tag="WJK66_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEZ7578236.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAIYCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEELGDPFKVSDADTM
                     LQFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(12280..14556)
                     /gene="metE"
                     /locus_tag="WJK66_000480"
     CDS             complement(12280..14556)
                     /gene="metE"
                     /locus_tag="WJK66_000480"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEZ7578237.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQAAANADYVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGAVEFDRLSLLPKLLPVYVEILTELAAAGAEWIQIDEPALAVDLPQAWL
                     DAYQDVYATLSQVNAKILLSTYFGSVAAHAGRLKALPVAGLHIDLVRAPEQLDAFADY
                     GKVLSAGVIDGRNIWRANLNNVLATLEPLKAKLGDRLWISSSCSLLHTPYDLSVEEKL
                     KVNKPALFSWLAFTLQKTQELRVLKTALNQGREAVAAELADSQAAADSRANSTEIHRA
                     EVAKRLADLPADADQRKSPFAERIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSDADYEAAMKKEIALVIEEQEKLNLDVLVHGEAERNDMVEYFGELLEGFAFTQY
                     GWVQSYGSRCVKPPVIYGDVSRPNAMTVAWSTYAQTLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLAWAS
                     EAFRLSSTGCEDSTQIHTHMCYSEFNDILPAIASMDADVITIETSRSDMELLTAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPFERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTRKLRAELAK"
     gene            complement(14694..15572)
                     /gene="metF"
                     /locus_tag="WJK66_000481"
     CDS             complement(14694..15572)
                     /gene="metF"
                     /locus_tag="WJK66_000481"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEZ7578238.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPPGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            15773..16048
                     /locus_tag="WJK66_000482"
     CDS             15773..16048
                     /locus_tag="WJK66_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEZ7578239.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            16048..16173
                     /gene="ykgO"
                     /locus_tag="WJK66_000483"
     CDS             16048..16173
                     /gene="ykgO"
                     /locus_tag="WJK66_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEZ7578240.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRKGKVYVICKSNPRFKARQR"
     gene            complement(16281..16481)
                     /locus_tag="WJK66_000484"
     CDS             complement(16281..16481)
                     /locus_tag="WJK66_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578241.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRRQKRALCEVAEISAWVLLG
                     AAAAMLFWYLFMLYFKHIPDSY"
     gene            16631..17770
                     /locus_tag="WJK66_000485"
     CDS             16631..17770
                     /locus_tag="WJK66_000485"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEZ7578242.1"
                     /translation="MSQNASVGIVTPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPVVTVKDWVKSQAALADYLGIGQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSHNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKECQQ"
     gene            17767..18348
                     /gene="metW"
                     /locus_tag="WJK66_000486"
     CDS             17767..18348
                     /gene="metW"
                     /locus_tag="WJK66_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEZ7578243.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTDSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            18465..19310
                     /locus_tag="WJK66_000487"
     CDS             18465..19310
                     /locus_tag="WJK66_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="d-alanine-poly(phosphoribitol) ligase"
                     /protein_id="HEZ7578244.1"
                     /translation="MKNKTSSLLLWLAAMMLTACSPSKEDKTKENGASAASSTASAAS
                     SSAPQTDLQPAASAPDNVKQAESVPPSNCTDLHPATGIDDLMQQIAEHIDSDCLFALS
                     HHELETRFGLPGGGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDA
                     LMEQERRLREATLLLIQGSQETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYI
                     GQLPGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNP
                     NLGRR"
     gene            complement(19360..19920)
                     /gene="efp"
                     /locus_tag="WJK66_000488"
     CDS             complement(19360..19920)
                     /gene="efp"
                     /locus_tag="WJK66_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEZ7578245.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKIEIDTRTGEFRKRA"
     gene            complement(19963..21111)
                     /gene="earP"
                     /locus_tag="WJK66_000489"
     CDS             complement(19963..21111)
                     /gene="earP"
                     /locus_tag="WJK66_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEZ7578246.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWTD
                     DVSALRALCPDLPDVPCVHQDIHVRTWHSDAADIDTAPVPDVVIETFACDLPENVLHI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYCEA
                     VRFDSEALRQRLMLPEKNAPEWLLFGYRSDVWAKWLEMWRQAGSPMTLLLAGTQIIDS
                     LKQSGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYTPETVSAHRRLSDDLNGGGALSAT
                     QRLECWQILQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(21130..21408)
                     /locus_tag="WJK66_000490"
     CDS             complement(21130..21408)
                     /locus_tag="WJK66_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578247.1"
                     /translation="MSRNLLVRWLVVCLIPLATLAVFAANPPEDKPQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMLLFIPIILLIVYLFHYFGAF"
BASE COUNT         5356 a         5512 c         5426 g         5343 t
ORIGIN      
        1 aatttaatcc actataaaaa ttcagacggc cttttcatta acaaataaaa ggccgtctga
       61 aacgattaca gatccaacaa caggcgggct gggtcttcca acgcgtcttt aatggctacc
      121 aaggtcaata cagcttcgcg gccgtcaatg atacggtggt cgtaagacag agccagatac
      181 atcattggac ggacaacaac ttggccgttt tcaaccacag cgcgctcttt agtggcgtgc
      241 atacccaaaa tcgcagattg aggcggattg atgatcgggg tggacatcat agaaccgaaa
      301 gtaccgccgt tggtaatact gaatgtaccg ccggtcagat cttcgatagc gattttgccg
      361 tctttggctt ttttcgcgta atcaacaatt gcttgttcga tgtcggcaat gctcatttgg
      421 tcggcatcgc gcaggattgg tacaaccaaa ccgcgtgggc tgccgatggc gataccgata
      481 tcgaagtagc cgtgatacac gatgtctttg ccgtcaacag aagcattcac aaccggatat
      541 tttttcaggg cggcaaccgc ggctttaacg aagaaggaca taaagcccag ttttacgccg
      601 tgttctttct cgaatttttc tttgtacttc gcacgcaagt ccatgattgg cttcatgttg
      661 acttcgttga atgtagtcag aatggcgttt tcttgttgag aagccaagag acgttctgca
      721 acacgggcac gcaggcggct catcggtacg cgttcttcgg gacgtgcgcc ggcaggaagt
      781 gtaacagcag gagcagcggc tgcggcaggt ttggcagcgg cattttgtac atcttctttc
      841 aatacgcgac cgtcacggcc ggaaccttgc aatgcgttca cgtcaacacc ggtttcggca
      901 gccagtttgg cggcggcagg catagcggcg ttgttttgtg cagcagcagg agcggcggcc
      961 ggggcagctt cggcaggagc ggcggcaggt gcttcagcag cggcagtagc agcggtatcg
     1021 atacgtgcca aaacttggtc ggcaacaacg gtttcaccgt cttgcgctac gatttcaacc
     1081 aatacgccgg cttgtggaga aggtacttcc aaaaccactt tgtccgtttc gatatcgatc
     1141 aggatttcgt cacgggcaac tgcttcgcca acttttttct tccattccaa gagcgtgcct
     1201 tcagatacgc tttcagacag cataggtact tttacatcaa taatcatttt gtgtctccaa
     1261 tggccctttc agacggcctg ttgtgtgttt ttcattctgc atatgggtgt tccgcctgag
     1321 cagcctcaga cggaatgcta cttggcttac aatgccaaag cgtcttcaac caattgtttc
     1381 aattgagcaa tgtgtttgct tgagtagccc actgcaggcg atgcgctgct tggacgaccg
     1441 gcataagaca gtttttgctc ttcgctgata acgtcttcga tgcggtggcg gatttggtag
     1501 aacgcgcctt ggtttttcgg ctcttcttgt gcccaaacca cagattttgc gttcggatat
     1561 ttcgccagtt cggctttaac ctcgtcgtat gggaacggat acagctgctc aacgcggaca
     1621 atagcgacat catcttccag tttacgttcg gctcgaccgg cttccaagtc atagtaaacc
     1681 tgaccggcac acaataccac gcgtttcacg ctgtcgttgc ttgcgcgttc ggcggtatcg
     1741 ccgataaccg gacggaaagt cgaaccttcg gtgaagtttt ccagcgggct cattgcccct
     1801 ttgaatcgca acaggcgttt ggacatgaaa atcactagcg gtttgcggta tgaacctaag
     1861 acttgacgtt gcaagaggtg gaacatttgc gacgcttcag acggcatgat gacttgcata
     1921 ttgttctcag aacacagttg caaccaacgt tctacgcgtg cagaagagtg ctcgggacct
     1981 tgaccgtcgt agccgtgcgg aaggatggtg gtcagaccgc acaaacgacc ccatttggtt
     2041 tcgcctgaag acaggaattg gtcaatagtc acttgcgcgc cgttggcgaa gtcaccaaat
     2101 tgagcttccc aaatggtcag cttgtcaggc gcggagcagg caaagccgta ctcgaacgcc
     2161 atcacggctt cttcgttcaa aatggagtcg ataaccaaga actcgcccat accttcgccc
     2221 atatggcgca gaggaacata agtaccgtca tcccattttt cacgtttttg atcgtgcaat
     2281 actgcatgac ggtgtgagaa tgtaccgcgc ccggagtcct caccggagat acgcacgcca
     2341 tgacctttgg ttacaaggct tgcgtatgcc aaggtttcgg ccatacccca gtcgatggct
     2401 tgtttgccgg atgccatggc tttacgcgct tcaatcacac gttttgcagt cggatgcagg
     2461 gcaaagcctt ccggtacggc ggtaaatttc tcggtaagac gctcaatatc cgcggcaggc
     2521 aaaccggttt cgatgtgttc gcgccaatct ttaccttggt atttgctcca gtcaatttgt
     2581 gtgcgttgga agttgctcaa cgttgtttgc tcgacgtgtt cgcctttatc caaagcatca
     2641 cggtaggctt ggatgtaacc gtcagcctcg gcttgggtta ccacgccttc ggcaatcagt
     2701 tgctcggtgt acaaagcacg cgcacccggg tgttgcgata cttttttgta catcatcggt
     2761 tgggtcaagg tcggatcgtc gccctcgttg tggccccatt tacggtagca gacaacgtcg
     2821 atcacgatgt ctttatggaa ttttttgcgg taatccaaag cggcttggat ggcaaagcaa
     2881 acacgctcag gatcatcgcc gttcacatgg ataaccggag cggaaaccat ttttgcgata
     2941 tcggtacagt gtacggttga acgggtatcg cggatatcgg aagtggtaaa gccgatttga
     3001 ttgttgatga cgatatgaac cgtaccgccg gtggtataac cgcgcgtttt agacagattg
     3061 aatgtcgctt ggttgacacc caaaccgata aatgcggagt cgccgtgaat caatacgggc
     3121 aagactttat cgcggccgtt ttcgcccaaa cgtttctgct tggcgcgtgc agaaccttcg
     3181 actaccgggt taacgatttc caagtgtgac gggttgaacg ccaaagaaac gtgcatcgga
     3241 ccatgtggag tggcgatgtc ggagctgaag cccatgtggt atttcacgtc gccgctaggc
     3301 aatttgattt cggcacggcc ttcaaactca gcgaacaaat ctccgggttt tttacccaaa
     3361 atgttcacca aaacattcag acggccacgg tgcgccatac cgatgatgac ttcttccacg
     3421 ccgtctttac cggcgttttg aatcaggtag ttcaaaccgg caatcgcgct ttcgccgcct
     3481 tcgacaccga aacgtttctg accgacatat ttggtatgca gataacgttc caaagtctcg
     3541 gcagcagtca tctctttcaa gatacggcgt ttttgatcgg cattgtaatg cggtgtggac
     3601 aatacgcttt caaaataatt gcgtacccag cggcgctctt cggtattggg aatatagata
     3661 tattccaatg cgatgtggcc gcagtaggtt tgtttgaggt tgctgatgat ttgggacaaa
     3721 ggcagtttgc cgcgattggc aaaatcgccc tcgcccatat tgaattgaag cgccatatcg
     3781 gcatctgaca gaccgtggaa tttcggatcg agggcttcaa tatcgcgcgg ggggatacgt
     3841 ttgagcggat caagttgggc tgcacccacg ccttggatac gataggcgga aatcagccgt
     3901 aaaacgctga cttgcttttt cagcattgcc tcatccgcac cgcccgcaac ggcagatgca
     3961 attttctttt tcgccaaagt aacaaatgat tcgcgaatcg gtgtgtgtgc gacatcgaca
     4021 gcaaccgtcc ccggctgttt gctcaaatcg gtgaaatact gcttccattt ttcatcaacc
     4081 gcatcggggt tttccaaaaa agcctcgtac aattcctcaa tgtaaggtgc gtttgaaccg
     4141 aacaggtaag agaaattgag tttttcgtcc atcatggcgt gcgctctttt ctttaaaata
     4201 taaacaaagg gtagaagtcc gcgtcctgac ggctaaactg atgccgcctg aaaagacatt
     4261 ttattctacc ctattttaaa ccggattgct tgtcttgatt tcatccgact acaatcagac
     4321 ggattcggga aacaatccgg ttctctgata ttgcctgttt gacaatatat taatgtatgt
     4381 tacctctcgc ctggcggcac ataatcgcgg cgttccgaac cagtataaag ctggcgcggg
     4441 cggccgattt tcagcgaagg atcgctaatc atctcgtgcc agtgcgaaat ccagcccacg
     4501 ctgcgcgaca gggcgaagat gacggtaaac atttcggtcg ggatgcccag cgcggacagg
     4561 acgatgccgg aatagaaatc gacgtttgga tacagtttgc gttcgataaa gaacgggtct
     4621 ttcagcgcaa tctgttccaa ttccatcgcc agtttgaatt tcggactgtc ttccaagccc
     4681 aattccttca aaacttcata gcaggtttcg cgcataatgc tggcacgcgg atccatattg
     4741 cggtacacgc ggtgaccgaa gcccatcaga cggtatttgc gttgtttcac accttccatg
     4801 tatgcggcaa cattagacac atcgccgatt tcgtccaaca ttttcaacac ggcttcgttc
     4861 gcaccgccgt gcgaagcacc ccacaggcag gcgataccgg cagcaataca ggcaaacggg
     4921 ttcgcacccg aagaccctgc cagacggacg gttgaagttg aggcgttttg ctcgtggtcg
     4981 gcatgcaaaa taaagatgcg gtcgagcgcg cgtgccaaaa cgggattggg tttgtagtct
     5041 tcacacggcg tggcgaacat catatgaagg aagttttcgg aataagaaag attattcttc
     5101 ggataattga acggcagacc gtttgaatag cggtagcaca ttgccgcaat ggtcgggatt
     5161 ttagaaatca ggcggtaaat cgcgattttg cggtgttcgg gattgctaat gtccaagctg
     5221 tcttggtaga acgcagacag tgcgccgacc acgccgacca tcatcgccat cggatgcgcg
     5281 tcgcggcgga acccccggaa gaaccaagtc agctgttcat gcaccatcgt gtggcggcgg
     5341 acggtattgt caaattctgc cttttgctcg ggagtcggca gttcgccgta aatcaacagg
     5401 tagcagactt ccaaataatc ggacttttcg gccagctgct cgatggggta tccgcgataa
     5461 taaagcaagc cttgatcgcc gtcgatgtaa gtaattttag actcacagct tgcggttgaa
     5521 acaaatccgg ggtcgaagga aaacaaacct gtattttttg tcagcccccg aatgtcaacc
     5581 acatcgtgcc cgatgctggc ttccaatacc ggcagctcca aacctgcctg acccggtacg
     5641 ttgagtttga ttgatttgga catattattg ctcctttgca agttataacc cacgttactg
     5701 catccgcagc acgttctttg ccttcagacg gcttttgcat tttgaaatct gcctgcaggc
     5761 agatttcaga tgcctcatgc ccgtctgatt ttttcaagca tcgggataag gtgccctttg
     5821 tccgtttccg aatgcccgtt aatcaaggca agcaattctt gatcttgaaa ttcaaggatt
     5881 tcggaaaact cggacagctc tttatcgctc aaatgctcga attctttttc cataaacctg
     5941 ccgaagatta aatctaattc caacaatccc cggcgggttt gaaaacggat tttccgtttg
     6001 gcaatatcgt caaaaaccat catttcttaa acggcccgtt tcaacataat ctctttaatc
     6061 ttaccgatgg ctcgggtcgg attcaagtgt ttaggacata cgtctacgca gttcataatg
     6121 gtgtggcaac ggaacaaacg gtatgggtcg ttcagattat ccaaacgctc attagtgatg
     6181 gtatcacggc tgtccgcaat gaaacggtaa gcattcagca aaccggacgg accaacgaat
     6241 ttatcagggt tccaccaaaa tgacgggcag gcagtcgaac agcaggcgca caaaatacac
     6301 tcgtacaaac cgtccaactc tttacgctct tcctgagttt gcagacgctc tttgtccgca
     6361 tcaatcggat tatcgttgac aacataaggt ttgacggaat ggtattgttt gaagaactgg
     6421 gtcatatcca caatcaggtc gcggataaca ggcagacctg gcagaggacg gattttaact
     6481 ggctgtttca agccacgcag atcggtcaaa cacgccaagc cgtttttgcc gttgatgttc
     6541 ataccgtccg atccgcaaat gccttcgcgg caggagcggc ggaaagacaa ggtatcgtct
     6601 tgtgctttca ggcgtaccaa agcatccaaa agtttcacgt cggtcggttc caattccaac
     6661 tcgtaacgct gcatataagg cttggcatca acatccgggt tgtaacggta aatttcaaaa
     6721 ctcatttttt ccatgagaag tgttcctttt ctcatcaact gcccaatgat tttgtattat
     6781 aaattcaagc agttcaatgt gggtatggtt agatttcaga cgagcttatg aggcgaaaac
     6841 cgtctgaaaa cgcatcaata aacgcgcttg gccggtttga tgtattccac gctcaaaggc
     6901 ttggtatgta ccggtttgta agacaaagta ttggtatctg aatggtacag cgtatgtttc
     6961 atccagtttt catcatcgcg ctcaggatgg tcgtctgaag cgtgcgcacc gcgtgattct
     7021 ttacgtgctt cggcagacac caaagtcgct ttcgccactt caattaggtt atccaattcc
     7081 aaagcctcga tacgcgcggt attccacact ttgctcttgt ctttgatttc ggtacgtttc
     7141 acacgctcgg caatcgccat gacttctcga acgcctttgc tcagaatctc atcagtacgg
     7201 aacacgccgg cgtgcaattg tacggagcgt tgcagttcgc ggcgcaatgc atcaacgttt
     7261 tcaccatcgg tttggctgtc caaacgctcg atacgttggc gggtcaactc accagcatta
     7321 gcaggcaaag gtttccagtc gctttgctct ttgatgaatt taatcatgct gtcgccggca
     7381 gctttaccga ataccaccaa gtccagcagg gagttcgtac ccaaacggtt cgcaccgtgt
     7441 acggaagcac aggcgcactc acctgcggca tacaggcctt ttacaggcac ttcgtattcg
     7501 tctccttgcg gcacaacgac ttcgccgtgg taattggtcg gaatgccgcc catcatatag
     7561 tgggtagtcg gcacaacggg aatcgggtct ttaatcggat cgataccagc gaactgaatg
     7621 gaaatctcgc ggatgcccgg cagtttttcc ataatttttt ctgcgccgat atggtcgatt
     7681 ttcagtaaga catggtcttt gtttttaccg caaccgcgac cttcgtagat ttccatcgcc
     7741 atcgcgcggg aaacaacgtc gcgagaagcc aagtctttta cggtcggcgc atagcgttcc
     7801 ataaagcgtt cgccgtcggc attcaacaga ataccgccct cgccgcgtac gccttcggta
     7861 atcaacacgc ccgcacctgc cacgccggtc gggtggaatt gccagaattc catgtcttcc
     7921 aacgggatac ctgcacgcgc acaaataccc aaaccatcgc cggtattcat ataggcattg
     7981 gtagaagacg cataaatacg gccgccgccg ccggtagcaa acatcacagc tttagcgtgg
     8041 aaaatataaa cttcgccggt ttccatttcc atggcggtta cgccgacgac atcgccgttt
     8101 tcatcacgaa tcaaatcttg tgccgtccat tccacaaaga attgcgtatt ggcacggacg
     8161 ttttgttggt acaaagtatg cagcatcgca tgacctgtac ggtcggcaac cgcacaggcg
     8221 cgttctaccg cgcgtttacc gtgttcggca gtatggccgc cgaaaggacg ctgataaatt
     8281 ttaccgcttt ccacacggtc aaaaggcata cccatgtgtt ccaactcaat tacggcttca
     8341 ggcgcggcgc ggcacataaa ctcaatcgca tcttggtcgc ccaaccagtc ggaacctttc
     8401 acggtatcgt acatgtgcca gtcccaacgg tcttcctgca cattacccag agaggcggaa
     8461 ataccgccct gcgccgctac ggtatgcgaa cgggtcggga acactttaga caaaacggca
     8521 caattcagac cggatttgga taattggagg gctgcgcgta aacctgcacc accaccgccg
     8581 acaatcacgg catcaaactt gcgaacagga aaacccatac ttacccccaa attactttaa
     8641 ttgaatacac caagcagccg accagccaga cgatggtggc aacctgcaaa aacaaacgca
     8701 cgccgaaggg tttgatatag tccatccaca aatcgcggat acccacccaa gcgtgcaaga
     8761 atacggcgat gaagctcact tgggtaaata cttttaccca agtttgacta aaaaatgcct
     8821 gccatgccga atattcttta ggcagggaaa atagaaccac taaaagtgca acggtataaa
     8881 tcaacataat aaccgcagtc gcacgttgca tcgcccaatc gcgcaaaccg taatgggcac
     8941 cggtcaattt acgttctacc ataacaacgc tcccaaaacg acagtcaaaa ccaatgcaga
     9001 agcaaatacg gctttagcgg tattgcgcgc agtattcagc tcaaggcctt tgtgtgcatc
     9061 caaaaataaa aagcggatac cggcgagaga atggtgcaga taagcccaca atacaccgat
     9121 taaaaccagc ttgaccaaag gatgggaaac aatggcacgg taagtttcaa atgcagactc
     9181 ttgactcagg gtaccggaca ggaaatacag caggaaaggc agcataataa acagcccgac
     9241 cccgctgatg cggtgaagga tggaaactat cccgggtatc ggcagacgga tgttcggcaa
     9301 atccagataa acaggacgcg gtttggcaga catagtcaat tcctctgtag ttatatatcg
     9361 actcaaaaac cgtaaaccca ttgctacact gggttacata aacgttaatt tacctgtttt
     9421 atcccatttt gaacagtgta ttttaaaaac aaactgatag attttgacaa acacgcgtgg
     9481 aaacagcaga ttaaaagaaa gatttttagt tttgccgccc ccttcccgac acggtatcag
     9541 catccgaccc catccccaaa aacaaaatgc cgtctgaaaa aatttcagac ggcattgttt
     9601 tgccatatcg gtcaggcttt caatttcgca tcggcatcgc gcaacgcggt acgcagacct
     9661 tcctcgataa cgggatggta gaacggcata tccagcattt gcggaacggt catcttcatt
     9721 tgatgcgccc atgccaacaa gtgcgccaaa tgttcggcgg cagggcctac gatttccgcg
     9781 ccgataaagc ggccggtggc tttttcggca tacaggcgca tatggccttt gttcaccagc
     9841 atcacgcggc tgcggccttg gtttttgaac gatacttcgc cgatgacaaa ttcgtcggct
     9901 tggtattgcg cggcaacctg cgcgtatttc agaccgacaa agccgatttg cggactggta
     9961 aacaccacgc cgatggtgct gcgccgcaaa ccgccgccga tattcgggta acggcctgca
    10021 ttgtcgccgg caatcttgcc ttggtcggca gcttcgtgca gcaggggcag ttggttggac
    10081 gcgtcgcccg caataaagat atgcggaatg ctggtctgca tggtcagcgg gtcggcaacg
    10141 ggtacgccgc gcgcatcttt ttcgatattg atattttcca aaccgatatt gtcaacgttc
    10201 ggacggcggc ccacggctgc cagcatatat tcggcaacaa atacgccttt ttcgccgtcc
    10261 tgctcccaat ggacttctac attgccgtct gcatcgagct tgacctcggt tttagcatcc
    10321 aaatgcagtt tcaattcttc gccgaacacg gctttcgcct cgtctgaaac gacggggtcg
    10381 gaaatgccgc cgatgattcc gcccaaaccg aaaatttcaa ctttcacgcc caaacggtgc
    10441 aatgcctgac ccagttccaa accgataaca cccggcccga acacggcaac gcgcttaggc
    10501 agcgtatccc atgagaaaac gtcatcgttg ataatcaaac gattgcccaa agactgccat
    10561 tgcggcagga tgacgggacg cgaaccggta gcaatcacga aactttttgc cgtaatttga
    10621 gtatgctcgt cgatttggac ggtatgctcg tcgataaatt tagccgaacc catgatgcgc
    10681 ttgtcggcag gccactcttc cacatcggca acgacaaagc cgacaaaacg gtcacgctcg
    10741 gatttgacgc gctgcatgac ctcttcgccg ttgacgacga tgctgtcttt gtccaaatgc
    10801 acgccgaacg ggtcggtatg caatgcgtga tgacgcgcct ctgcggcggc aatcaagagt
    10861 ttggaaggca tacagcccac gcgcgcgcag gtcgtgccga acacattgtt ttcaatcagg
    10921 taaacattat ccgaatgtaa acgggcattg cgaaacgcac ccataccggc agtaccgccg
    10981 ccgattacga cgacatccgc ttgaattttt ttcataatct ttgtccttcc gttttcgtct
    11041 gaaacattgc cgacacagaa catatcggtt tcagacggca tttaaatatt ttcccaatca
    11101 aaaacatcat tcagataaat aaaaacaaaa tttcgattga gtgcaacaat atccaaattc
    11161 tcttataaat atagagttat tcggagcggc agtgtagaac cgcccgaata actctatccg
    11221 gtatatcaga cagtttaatc aaaccgcctt aagcaaacag ctatcagttt ttagccaagt
    11281 aagcttccaa atcttcgctg ccgccgatgt atttgccgcc gatgaagact tgaggggcag
    11341 tcatcttgcc ggtaatggcg cgaacggaag taacggttgc atctttgccc aatacgattt
    11401 cttcgtaaga caaacctttg tcttgcaaag cttgtttggc tttagcgcag aattggcaac
    11461 ctggtttagt gaaaattgcc acagactctt gagccttcca atcgggagca acgaattgca
    11521 gcatagtatc tgcatcggat actttgaacg gatcgcccag ttcttcaggt tcgatgaaca
    11581 ttttttcaac cacgccgtcg ttaaccagca tggagtaacg ccaagagcgt ttaccgaagc
    11641 ccaagtcttc tttaccgacc agcataccca taccttcggt aaattcgccg ttgccgtcag
    11701 gaatcatgta gatgttgtcg gattcttctt cggcagccca agcgttcatt acgaacgtat
    11761 cgtttacaga tacgcagtag attgcgtcaa cgccgttttc tttgaacgcg ccgaacaatt
    11821 cgttgtaacg cggcaggtgt gaagaagaac aagtcggggt aaatgcaccg ggcagggaga
    11881 atacgactac ttttttgcct ttgaacaaat catcggtaga cacatctttc caagtgtcgc
    11941 cgacgcgggt gcggaatact acggaaggta ctttttgacc ggtacgatct tgcaaagcca
    12001 ttttaagctc cttaatagat tggtttgaga aataattgtg aatcgctggg cattctaccc
    12061 catgcaaaat gatttgtata atttatagtt caaataatat aaattattta aaactatcaa
    12121 acacaactcc gatttgcctg tttcccacat ataaagccga attagaaaaa aacaagctgc
    12181 aatgaaaaga ttttccggca actgcgccct tgcgccgcaa atcaggacaa aataccgtct
    12241 gaaaggcttt cagacggtat ttattcatac ggtctcggct tatttcgcca attcggcacg
    12301 cagttttcgg gttacgttca tcatcacttg gagttgttcc agagtttctt tccagccgcg
    12361 tgttttcagg ccgcagtctg ggttaaccca cagacgttca aacggtacaa cctcgatggc
    12421 tttgcgcaac aggtgctcaa cttcggcttc agtcggtacg cgcgggctgt ggatgtcgta
    12481 aacgcccggg ccgatgtcgt tcgggtattt gaattcgccg aatgcggtca acaattccat
    12541 gtcagaacgt gaagtttcga tggtaatcac gtccgcgtcc atgctggcga tggccggcag
    12601 gatgtcgttg aattccgagt aacacatatg ggtgtggatt tgggtactgt cttcgcaacc
    12661 ggttgacgac aggcggaagg cttcgcttgc ccaagccaag taggcatccc aatcggcacg
    12721 tttcaacggc aggccttcgc ggatggccgg ttcgtcgatt tgaatcactt taataccggc
    12781 tttttccaaa tccaatactt cgtcatttaa ggccaaggcg atttgtttgc acacggtgct
    12841 gcgcggaata tcgttgcgca caaatgacca ttgcagaatc gttaccggac cggtcaacat
    12901 gcctttcatc gggcgtttgg tcagggtttg ggcgtaggtt gaccaagcca cagtcatcgc
    12961 gttcgggcgg ctaacatcgc cgtaaatcac cggtggtttc acgcagcgtg agccatagct
    13021 ttgtacccag ccgtattggg taaaggcaaa gccttccagc aattcgccga aatattccac
    13081 catgtcgtta cgctcggctt cgccgtgtac caacacatcc aaattgagtt tttcttgctc
    13141 ttcaatcacc aaggcgattt cttttttcat cgcggcttcg taatcagcgt cagacaattc
    13201 gccttttttg aacgcggcac gcgcttggcg gatttcagtg gtttgcggga acgaaccgat
    13261 attggtggtc ggcagcaacg gcaggttcag ccatgcttgt tgcgctttaa tgcgctcggc
    13321 aaacggtgat ttgcgttggt cggcatctgc cggtaaatca gccaagcgtt tggccacttc
    13381 ggcgcggtgg atttcggtgc tgttggcgcg tgaatcagca gcggcttggc tgtcggccaa
    13441 ttcggcggca acggcttcgc ggccttggtt caatgcagtt ttcaacacgc gcaattcttg
    13501 cgttttctgc aaggtgaaag ccaaccatga gaacaaggca ggtttgttga ctttcagctt
    13561 ttcttctacc gacaaatcgt atggcgtgtg cagcagcgag catgagctgg aaatccacaa
    13621 gcggtcgccc aatttggctt tcaacggctc aagcgtggct aatacgttgt ttaaattggc
    13681 gcgccaaata ttacggccat caatcacacc ggcagacaac actttgccgt aatcagcaaa
    13741 tgcgtccaat tgctcgggcg cgcgcaccaa atcgatgtgc aaaccggcca caggcaaggc
    13801 tttcagacga cctgcgtgtg cggcaaccga accgaaataa gtgctcaaca agattttggc
    13861 attcacttgg ctcaaagtgg cgtaaacgtc ttggtacgca tccaaccacg cttgcggcag
    13921 gtcaaccgcc aatgctggct catcgatttg aatccattcg gcaccggcag cggccaattc
    13981 ggtcaggatt tcaacataaa ccggcaataa ttttggcaac aggctcaagc ggtcaaattc
    14041 gacagcgcct ttttctttac caacccacag gaaggtcaac ggaccaacaa cggtcggttt
    14101 ggctttcagg cccaaagctt gggcttcttg cagctgttga acgtagtgtt tggcgttcgc
    14161 tttgaattcg gtatcggcgt ggaattcagg caccaagtag tggtagttgg tgtcgaacca
    14221 tttggtcatt tcgatagcga attggtcttt attgccgcgt gccagttgga agaattgttc
    14281 caaagacagg ttttggctgt cgaagccgaa acgggcaggg atggcaccgg tagcgacttg
    14341 caggtcgagg atgtggtcgt agaaagtgaa atcgcctaca gcaacgtaat cagcgttggc
    14401 agcggcctgg tgtttccagt ttttctcgcg caagtcttta gcaacagcca gcaattcttg
    14461 ctcgctgatt tctttgcgcc agtatttttc ttgtgcgaat ttcaattcgc ggaaggcgcc
    14521 gacacgcggg aagcctgaga aatgtaatgt tgtcatgttg aactctccag ttggattttg
    14581 taaggaccgg ttcaatccga atcggccggt attgtaaatc agaatggctc ggatggggcg
    14641 gtgttttgct ttttagacac cttagatgcc gtctgaaact gtttgatacg gctttaaggg
    14701 cgcacgccta aaatatggca aatggcgtaa gtcagctcgc tgcggttaag cgtatagaag
    14761 tggaaatctt tcacgccttc gcgggacagg actttgacca tatcgatggc gatacttgcc
    14821 gccaccagat tgcgcgtacc ttggtcgtcg tccaaacctt catacatttg cgacagccag
    14881 cttgggattt tgacgttggt tacttgagcc attttaccga gctgcttgaa gttggtaaca
    14941 ggcaaaatac cggggacgat ttccacatcg atgcccaaca tcacgcagcg gtcgcggaag
    15001 cgcaggtagc gttccacatc gaagaagaat tgggtgatga cgtggttcgc gcccgcatcg
    15061 attttgcgtt tcaaattaat caggtcggct tgtgcggatt tcgcttcggg atgcacttcg
    15121 ggatatgctg ctacagaaat gtcgaagtcg gcgacggagc gtaatagctt aaccaagtct
    15181 tcggcataaa acggtttttt ctcgtaaccg ggcggctcgt cgccgcgcag ggcgacaatg
    15241 cggcggatgc cgctgtccca ataatctttg gcaatttggc gcaattcgtc gggagaagcg
    15301 tcgatgccgg tcaggtgcgg cgcggcttcc aagccggttt cttgcttaat gcgtttgacg
    15361 atgccgtgcg tgcggtcgcg ttcgccggaa tttgcgccgt aggttacgga tacgaacttg
    15421 ggatgcaggg tttgcagacg gtggatggaa tcccacagca tcgtttccat ttgttcgttt
    15481 ttcggcggga aaaattcaaa cgaaacgttg atgtcgcctt tcaaatcgga aaggctgttg
    15541 tttaacgcat tgatttcttt tgcgtaattc atggctgcgt accttttatt atcttttata
    15601 tgatatttca ggcaacataa taaaattgca ccgaatatga gtcaatttca aaattttcag
    15661 gtttatcatg aaaatttcta atatattaca aaaatagtta aatttccttg tacaagcctt
    15721 ctaaatttat gataaagttt gttatgttat aacaatccaa ctaaggcgat atatgaaacc
    15781 gaatattcat cctgacaatt atcgaaccgt cctttttttc gacagcagcg ccaatgaagg
    15841 ctggctgatt cgttcttgcg ccgaaacaca cggaaaaacg atggtttgga cagacggcaa
    15901 agaatatcca ctgttttcgc tggatacttc ttccgcatcc catcccgtct ataccggcaa
    15961 gcaacgcaac gtcaacaccg aaggccgtgc cagcaaattc aaccaacgtt tccaatccgt
    16021 catgtcatcg tttagaaagg acaaataatg caggttctgt cttcactcaa aaccgccaaa
    16081 caacgccacc gcgactgtca gattgtcaga cgcaaaggca aagtttacgt catctgcaaa
    16141 agcaatccgc gttttaaagc gcgccaacgc tgaccccctg cccttcctgc ttcggttgta
    16201 aatcccctcc taccgcaagc ccttttcatc aaatatgccg tctgaaacag aaaaatgcgt
    16261 ttcaggcggc attttttccg tcaatacgaa tccggaatgt gtttgaaata cagcataaac
    16321 agataccaaa acagcatcgc ggcggctgca ccgagcagta cccatgcgct gatttcggcg
    16381 acttcgcaca gggcgcgttt ttgcctgcgg ctgaaaaggc ggttgataat caacgcggca
    16441 agtgcgaaaa agaaaaaaat acggaaaagt ctgcctatca tgtcgggaag cggtggaggt
    16501 cgtctgaaaa agtatgattc gtgtgcggcg gatgagggtc aaacgcgtcc ggctgcttgt
    16561 ccgaccgtgt caaaaaatca tgccgtttgc gatataataa cgcttttcgc cgcatttgtt
    16621 ggaacaggat atgagtcaaa atgcctcggt gggcattgta acgccccaaa aaattccgtt
    16681 tgaaatgccg ctggttttgg aaaacggtaa aactttgccg cgtttcgatc tgatgattga
    16741 aacctacggc gagctgaatg ccgaaaaaaa taatgcggtt ttaatctgtc atgcgctgtc
    16801 aggcaaccat catgttgcgg gcaggcattc ggcggaggat aaatatacgg gctggtggga
    16861 caatatggta ggacccggca aaccgattga tacggaacgt tttttcgtgg tcggtttgaa
    16921 caatctgggc ggctgtgacg gcagcagcgg acctttgtcg atcaatcctg aaacgggtag
    16981 ggaatacggc gcggattttc cggtggttac ggtgaaggac tgggtaaaat cacaagccgc
    17041 gcttgccgac tatctcggca tcgggcaatg ggcggcggtt gtcggcggca gtttgggcgg
    17101 tatgcaggct ttgcagtggg cgatttccta tcccgaacgc gtgcgccatg ccttagtgat
    17161 tgcgtctgcg ccgaaactgt ccacgcaaaa tatcgcgttc aacgatgtgg cgcgtcaggc
    17221 gattttgacc gaccccgatt tcaacgaagg acattaccgc agccataata ccgttcctgc
    17281 acggggcttg cggattgccc gcatgatggg gcacattacc tatcttgccg aagacggttt
    17341 gggcaaaaaa ttcggacgcg atttgcgttc caacggctat caatacggtt atggcgttga
    17401 atttgaagta gaatcctatc tgcgctatca gggcgacaaa ttcgtcgggc ggtttgatgc
    17461 caacacctat ttgctgatga ccaaagcttt ggactatttc gatccggcgg cggatttcgg
    17521 caacagcctg acccgcgccg tgcaggatgt gcaggcaaaa ttctttgtcg ccagcttcag
    17581 caccgattgg cgtttcgcgc ccgaacgttc gcacgaactg gtcaaggccc tgattgccgc
    17641 ccaaaaatcc gtgcagtata tcgaagtcaa atccgcacac gggcacgatg cctttttaat
    17701 ggaagacgaa gcctatatgc gtgcggtcgc cgcctatatg aacaacgttt ataaggaatg
    17761 tcagcaatga acctgcgcga tgatttgcaa ctgatttacg accggatacc cgaaggcagc
    17821 cgcgtcttgg acttgggctg cggcgacggc gaactgcttg ccgcattggt cgaacacaaa
    17881 aaatgcagcg gctacggcat cgaaatcgac accgacagcg tgattgccgc gatgtcgcgc
    17941 ggcgtgaatg ttatccaagc cgatttggaa gaaggtttaa ccgcattcaa cgatcaaagt
    18001 tttgatgtga ttgtgttgag ccaaaccatc caagcgatgc agaataccga aaaaatcctg
    18061 cgctgcctga tgcgcgttgc caaacaggcg attgtcagct tcccgaattt cggctactgg
    18121 cgcaaccgcg tccaaatcgc actcggcgga catatgccgg tttccgaacg tatgccctac
    18181 cattggtacg acacgcccaa tatccattgg tgcacgctca aagactttga tttgttgtgc
    18241 gccaaaaaca acatccgcgt cctcgaacgc gccgtgatga cgggcaacag acaggtcaaa
    18301 catttcccca accttttggg cagccttgcg ttttaccgcg tcggctaaga caggttgaga
    18361 accttgtcgg caataacagg ttcttcggtt ctattaacaa caggccgtct gaaaaatgaa
    18421 acggtaggtt ttcagacggc ctctttaata ctggaaacaa tagaatgaaa aacaaaacct
    18481 catcacttct cttatggctt gccgcaatga tgctgaccgc gtgttccccg agcaaagaag
    18541 ataaaacgaa agaaaacggc gcatccgccg cctcgtccac ggcatccgcc gcttcgtctt
    18601 ccgcgcccca aaccgatttg caaccggccg catccgcccc tgataacgtc aagcaggcag
    18661 aaagcgtgcc gccgtcaaat tgcaccgacc tgcaccccgc caccggcatt gacgatctca
    18721 tgcagcaaat cgccgaacac attgactcgg actgtctgtt tgccctttcc catcacgaac
    18781 tggaaacccg tttcggctta cccggcggcg gctatgacaa catacagcgg ctgctgtttc
    18841 ccgacatccg ccctgaagat cccgactacc atcagaaaat catactggca attgaagact
    18901 tgcgttacgg aaagcgcacg atcagccggc aggcacaaga tgccttgatg gaacaggaac
    18961 gccgcctccg agaagcgacg ctgttgctga tacagggcag tcaagaaacc cgcggacaag
    19021 gcgaggagcc gaaacgcacg cgttattttg aagtttcggc aacccctgcc tattcgagcc
    19081 ggcacaacaa cggacttggc ggcaatttcc aatacatcgg ccaattgccc ggctatctga
    19141 aaatacacgg agaaatgctt gaaaaccaat cactcttccg gctgtccaac cgtgaacgca
    19201 atcccgacaa accgttttta gacatccatt ttgacgaaaa tggcaaaatc acgcgtattg
    19261 tcgtttacga aaaaaacatc tacttcaatc caaacttggg gcgaagataa aaaaatgccg
    19321 tctgaaacgt tttcagacgg catttttcaa tgcaacaaat caagcgcgtt tgcggaattc
    19381 gccggtgcgg gtatcgattt cgattttgtc gccgttttcg atgtaagaca tcacttggat
    19441 ttcggtgccc ccgaccaggc gcgcggtttt catcactttg cctgaagtat cgcctttgac
    19501 ggcaggctcg gtatactcga cttcgcgtac gatgatggtg ggcagttcta cggagatggg
    19561 gttgccttcg tagaaggtaa cttcgcattg gtcttccatg ccgtcaacga tgaatttcaa
    19621 cgcgtcgccg atgttgtcgg cttcgatttc gtattggttg aattcttcgt ccataaagac
    19681 gtacatcgga tcggcaaagt agctgtacgt acagtttttg cgggacagga tgaccacgtc
    19741 gaatttgtcg tcggctttgt aaatggtttc ggaagccgcg ccggtcagca ggtttttcag
    19801 tttcatgctg actttggcgg aagagcggcc gcctttgatg tattcggttt tttgaacgac
    19861 cataggatcg ttgccgacca taaatacatt gccggcgcgc agttcttgtg ctgttttcat
    19921 tgtgattttc caatcaaatc ggttgtcgta aaacgcacta ttctagcgta ttttttgatg
    19981 ctttgaaaca aaggcggcga gtttttcgga tgcggaaggc tgcccgaaaa gataacggct
    20041 ccaatcctcc gcgccttgcc gccagccgtt ttgatgttgt tgcaggattt gccaacattc
    20101 gaggcgttgc gtggcggata aagcccctcc gccgttgagg tcgtccgaaa gacggcggtg
    20161 tgccgacacg gtttcgggcg tgtagaaacc gtgtgcctta tcccaaaagg cgtggagttt
    20221 gtcgagatgg acattctcgt cttgcgggta gatgtgccaa aagaagggtt tgccggcaag
    20281 ctgggcgcgc acgaaactgt cttcgccgcg tatcacggcg cagtcggcga ggtgcagcag
    20341 tttgtcgaag tcctgttgcg gcacgaaagg gattttgacg aggcggacgg atgccgtctg
    20401 aaaaacatcg ccgtcgtttt gcagggcatc ttgcggaata acgccgcttt gtttgaggct
    20461 gtcgatgatt tgcgtccccg ccagcaacag tgtcatcggg ctgcctgcct gtcgccacat
    20521 ttccagccac tttgcccaaa catcgctccg atagccgaaa agcagccatt cgggggcgtt
    20581 tttttcggga agcatcagcc gctgacgcag ggcttcgcta tcgaaacgga cggcttcgca
    20641 gtaatcgcgt tcgcgtatca acccgccgct tttttcgctg aaacccataa accaaaaata
    20701 tttttgaaca ccttcctgcg gcgaaggcat caggtgcagc ctttcattgc tttcctccgc
    20761 gctcaaatat tcccaattca gccaaagcgg cttgtgtcgg cggataatgt gcagcacatt
    20821 ttcgggcagg tcgcaggcaa aagtttcgat gacgacatcg ggaacaggcg cggtatcaat
    20881 atctgccgca tcggaatgcc aagtgcggac atgaatatcc tgatgaacgc agggaacatc
    20941 gggcaaatca gggcaaagcg cacgcaaggc ggacacatcg tccgtccaca aatgcacctg
    21001 ccaaccgagt tcgcggtgca aaacacgggc gagccgccac gaaacgccga tgtcgccgaa
    21061 attgtcgatg accttgcaaa aaatccaaca gacaaaagga ggagtattca tagaaataac
    21121 ggaaacgggc taaaacgcgc cgaaatagtg gaacaaatac acaatcagca aaataatcgg
    21181 aataaacagc aacatagttt gacgtttcaa atcaaactct tgcttcaaat gatgcttgat
    21241 ggtgtcgaaa aggacgaatt tgaacaaaaa cgtcgcttcg caggcaagga tgatgccgtt
    21301 aatcagatgc tggggtttgt cttcgggcgg attggcggcg aaaacggcaa gcgtcgccaa
    21361 gggtatcagg cagacgacaa gccagcggac aagcaggttt cgggacattg caaggctttc
    21421 aaaaaagcaa aacgccatta tcgccccaag cgcacgcctt gtctaacgga tgcgccggag
    21481 atgccgtctg aagccttgcc gcaggaaaac accgcaccgc cgaaccttta aatacgcccg
    21541 agacgggcgt tccccgaaaa atcggacgct gccgccgatg cctgaggaca aggcggcagc
    21601 aggcacgggc atattgaaaa cccggcgcgt cggtttg
//
LOCUS       DAWKOG010000017        21585 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.017, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000017 DAWKOG010000000
VERSION     DAWKOG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21585
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.017"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(135..2021)
                     /gene="uvrC"
                     /locus_tag="WJK66_000491"
     CDS             complement(135..2021)
                     /gene="uvrC"
                     /locus_tag="WJK66_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEZ7578248.1"
                     /translation="MPSEAVFQIIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GKVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSEAEALILENNLI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYRDSVRQAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGIVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFSVPDALKEALEGEHGKQMQFVTKTIGERKVWLKMAEQNAQMAIAQRRLQQSNQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKMQEAEANGETVKWPDAVLIDGGKGQIGIAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     EHLH"
     gene            complement(2076..4253)
                     /locus_tag="WJK66_000492"
     CDS             complement(2076..4253)
                     /locus_tag="WJK66_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEZ7578249.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVKSAGVNFASEEAQ
                     VTFDDSKTSAADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLLAINIPFLIG
                     MVGMMLKGLNWTRHDWMLSPLLQFALASVVQLWLAVPFYKSAWASIKGGLANMDVLVT
                     IGTVSIYLYSVYMLFFSPHAAYGMAHVYFEVGIMVIGFVSLGKFLEHRTKKSSLNSLG
                     LLLKLTPTQVNVQRDGEWRQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHL
                     TGESNPEEKKAGGKVLAGALMTEGSVVYRAAQLGSQTLLGDMMNALSEAQGSKAPIAR
                     VADKAAAVFVPAVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAI
                     MVGIGKAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGSPQVAAVYCVPDSGFDED
                     ALYRIAAAVEQNAAHPLARAIVSAAQARGLDIPAAQNAQTVVGAGITAEVEGVGLVKA
                     GKAEFAELALPKFLDGVWDIASIVAVSVDNKPIGAFALADALKADTAEAIGRLKKHNI
                     DVYIMSGDNQGTVEYVAKQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDA
                     PALAAANVSFAMKGGADVAEHTASATLMQHSVNQLADALSVSRATLRNIKQNLFFAFF
                     YNILGIPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRVKID"
     gene            4392..5342
                     /gene="trxB"
                     /locus_tag="WJK66_000493"
     CDS             4392..5342
                     /gene="trxB"
                     /locus_tag="WJK66_000493"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEZ7578250.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            5498..5866
                     /gene="rpsF"
                     /locus_tag="WJK66_000494"
     CDS             5498..5866
                     /gene="rpsF"
                     /locus_tag="WJK66_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEZ7578251.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAVAQ"
     gene            5867..6169
                     /gene="priB"
                     /locus_tag="WJK66_000495"
     CDS             5867..6169
                     /gene="priB"
                     /locus_tag="WJK66_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEZ7578252.1"
                     /translation="MGFNNLVSLAALIEKVFPIRYTPAGIPVLDIILKHKSWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            6176..6406
                     /locus_tag="WJK66_000496"
     CDS             6176..6406
                     /locus_tag="WJK66_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEZ7578253.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            6423..6875
                     /locus_tag="WJK66_000497"
     CDS             6423..6875
                     /locus_tag="WJK66_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEZ7578254.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARVRQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            6889..6996
                     /locus_tag="WJK66_000498"
     CDS             6889..6996
                     /locus_tag="WJK66_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HEZ7578255.1"
                     /translation="MPSETLPIVGMWFQTVFVLFFVYSAKIGMVVFIVD"
     gene            complement(7663..8469)
                     /locus_tag="WJK66_000499"
     CDS             complement(7663..8469)
                     /locus_tag="WJK66_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ7578256.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQAVDWKTVFTMMPGMVFGVFAGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTDPQTASRPLPGLPGLTAVSTLFGTMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(8470..9216)
                     /gene="pssA"
                     /locus_tag="WJK66_000500"
     CDS             complement(8470..9216)
                     /gene="pssA"
                     /locus_tag="WJK66_000500"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEZ7578257.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            9799..10282
                     /locus_tag="WJK66_000501"
                     /pseudo
     CDS             9799..10282
                     /locus_tag="WJK66_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
     gene            complement(10334..11545)
                     /locus_tag="WJK66_000502"
     CDS             complement(10334..11545)
                     /locus_tag="WJK66_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HEZ7578258.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQVNWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGIKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            11768..14206
                     /locus_tag="WJK66_000503"
     CDS             11768..14206
                     /locus_tag="WJK66_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEZ7578259.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVMLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAGAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDNPEPLEKLPMIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHHVVAFVKEQAPANYVEGLLTGEAAQETAN
                     IVSPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSPSD
                     LNGSRKILAHKDHL"
     gene            14414..15724
                     /locus_tag="WJK66_000504"
     CDS             14414..15724
                     /locus_tag="WJK66_000504"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEZ7578260.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFHDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGASQMLPEFEAGVVGMKAGESKDVTVNF
                     PEDYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVKKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YAEPSRLQAPTSLAIESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            15820..16434
                     /gene="clpP"
                     /locus_tag="WJK66_000505"
     CDS             15820..16434
                     /gene="clpP"
                     /locus_tag="WJK66_000505"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEZ7578261.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQVLENRASLQL"
     gene            complement(16487..17752)
                     /gene="ispG"
                     /locus_tag="WJK66_000506"
                     /gene_synonym="gcpE"
     CDS             complement(16487..17752)
                     /gene="ispG"
                     /locus_tag="WJK66_000506"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEZ7578262.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDNIATEFL
                     AIVEEYVKTNYGENGSKRNQNKIIPIQSL"
     gene            complement(17768..18529)
                     /gene="pilW"
                     /locus_tag="WJK66_000507"
     CDS             complement(17768..18529)
                     /gene="pilW"
                     /locus_tag="WJK66_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEZ7578263.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLIGQ"
     gene            complement(18532..19626)
                     /gene="rlmN"
                     /locus_tag="WJK66_000508"
     CDS             complement(18532..19626)
                     /gene="rlmN"
                     /locus_tag="WJK66_000508"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEZ7578264.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFNE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVTDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(19770..20195)
                     /gene="ndk"
                     /locus_tag="WJK66_000509"
     CDS             complement(19770..20195)
                     /gene="ndk"
                     /locus_tag="WJK66_000509"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEZ7578265.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPSEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            complement(20254..21405)
                     /locus_tag="WJK66_000510"
     CDS             complement(20254..21405)
                     /locus_tag="WJK66_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1 family ATPase"
                     /protein_id="HEZ7578266.1"
                     /translation="MSNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
BASE COUNT         5192 a         5328 c         5872 g         5193 t
ORIGIN      
        1 agcaccttag agaatcgttc tctttgagct aaggcgaggc aacgccgtac tggtttaaat
       61 ttaatccact atatatcggt ataccgacct gctgtatcat attttttaca gacggttttt
      121 gcttgggcag catactaatg cagatgctcg taaatggttt ccgccaatgc cttgctgatg
      181 ccttccactt tttccaagtc ctcgcggctg gcggcaatca cgccgcgcag accgccgaag
      241 cgggtgagca gggcttggcg gcgtttgctg cctacgccgg ggatgtcgct taaggaggag
      301 gtaacgcggg ctttgtcgcg ttttttgcgg tggccggtaa tggcgaaacg gtgcgattca
      361 tcgcgtacgg tttgcaatag atgcaaggcc gggctgttgg gcggcaggcg gaagacttcg
      421 ccggtaaaag gcagtatgag ttcttccata ccggctttgc gttcggggcc tttggcgata
      481 ccgaccaaag gaatgtgcag cccgagttct tcccataccg atacggcgat gccgatttgc
      541 cctttgccgc cgtcaattaa cacggcatcc ggccatttga cggtctcgcc gttggcttcg
      601 gcttcctgca ttttgccgta acggcgcgtc aacacttcgc gcatggcggc gtagtcgtcg
      661 ccgggtttgg cggtcgtgat gttgtagcgg cggtattgcg agggctggat gttttgctca
      721 tcgtacacga cgcaggaggc gatggtggct tcgccttggg tgtggctgat gtcgaagcat
      781 tcgaggcggt tgaggccgtc tgaatccata ccgaggattt tggcgagttc gtcgatgcgg
      841 tgttgctggt tgctttgttg caggcggcgt tgtgcaatcg ccatttgtgc gttttgttcc
      901 gccattttca accaaacttt gcgttcgcct atggtcttgg tgacaaattg catttgtttg
      961 ccgtgttcgc cttccaaagc ctctttcagc gcatcgggaa cggaaaagtt gctaataatg
     1021 atgtcgggtt tgcttttgcc caaataatgt tgggcgacga aggcttcggc gtaatcttgt
     1081 ccgtttggct cgggatcgtt tttggtgtcg gggaagaaac ttttgtctcc gacgtgccgc
     1141 ccaccgcgta tgctgaccca gtgtacgcag acgatgccgt ctgaaaccgc cagcgcgagc
     1201 aaatcgatat cgttggggtt gttcgggttt ttgctgtcga tgaactggtt gctctgcata
     1261 atgccgagtg cttggatttg gtcgcggtag cgggcggctt cttcaaattg cagattcgcg
     1321 gcggcagttt gcattttgtg ttgcagggtg cgggtcagtt cgtcggtttt gccattgaga
     1381 aaagtggcgg cttgacgcac gctgtcgcgg tagtcttctt cgctgatgtg tccgacgcag
     1441 ggcgcggtgc agcgtttgat ttgatacagc aggcaagggc ggtcacgatg ctcgaatacg
     1501 ctgtcttcgc aggtacgcag cataaagact ttttgcaaca cttgaatgct gtcgcgcacg
     1561 gcgttgctgt tgggatacgg gccgaagtat tggtttggct ttttcagcgt gccgcggtaa
     1621 tacgccatct gcggatattg atggccgctg agcatcaaat aaggatagct tttgtcgtcg
     1681 cggaaaagga tattgtattt cggcgacaag gctttgatga ggttgttttc gagaatcagg
     1741 gcttcggctt cggaacgcgt gatggtggtt tcgatatgat gaacctgttt taccatcaat
     1801 gcgatgcgcg gcgaatggtc gtttttttgg aaatagccgg acacgcgccg cttgaggttg
     1861 accgctttgc cgacgtataa gactttgccg ccttcgtcaa aaaaacggta cacgcccggc
     1921 agcttgggca ggtttttgag gaagagcggg atatcgaatt gttccggaga acggttttct
     1981 gtattcatat caattatttg aaaaacggct tcagacggca tatcggcagc ggcagcctta
     2041 tccgttcgga aaagctgtcg ggtggttata tgcggtcaat cgatttttac ccgtttcagg
     2101 cgcaaggcgt tgctcaacac ggaaaccgag cttgccgcca ttgccgcgcc tgcgatgaca
     2161 gggtttaaaa aaccgagcgc ggcaagcgga atgcccaaaa tattgtagaa gaaggcgaaa
     2221 aacaggtttt gcttgatgtt tctcaacgtc gcccgcgata ccgatagggc atcggcgagt
     2281 tggttgaccg aatgttgcat cagcgtggcg gatgcggtgt gttcggcaac gtccgcgccg
     2341 cctttcatgg cgaagctaac gttggcggcg gcgagcgcag gtgcgtcgtt gatgccgtct
     2401 ccgaccatcg ccacggtttt gccggcggct ttgagtttct gcacttcggc ggctttgtcg
     2461 cgcggactca tattaccgaa ggcgtgtgcg atgcccagtt gtttggcgac gtattcgacc
     2521 gtgccttggt tgtcgccgct cataatatag acatcgatat tgtgtttttt cagacggcct
     2581 atggcttcgg cggtatcggc tttcaacgcg tcggcaagtg cgaatgcgcc gatgggtttg
     2641 ttatcgactg agaccgcaac aatgcttgca atatcccaaa cgccgtctaa aaacttcggc
     2701 aaggccagtt cggcaaattc ggctttgcct gctttcacca aacccacgcc ttccacttcg
     2761 gcggtaatgc ctgcgccgac aacggtttgt gcgttttgtg cggcgggaat gtccaaaccg
     2821 cgcgcttggg cggcggagac gatggcacgg gcgagcggat gggcggcgtt ttgttcgacg
     2881 gcggcggcga tgcggtacaa agcgtcttcg tcaaagccgc tgtcgggaac gcaataaacg
     2941 gcggcaacct gcgggctgcc ttcggtcagc gtaccggttt tgtccaacac gacggcatcg
     3001 acgtgggcgg cttcctccat tgctgccgcg tctttaaacc aaataccgtg tttcaccgct
     3061 ttgccaatgc cgaccataat cgcggcgggt gtagccagac cgagcgcaca cgggcaggca
     3121 atcaccaaaa cggcgacggc gtgcatcagc gcaaccgtcc aatcgccctt aatcagccaa
     3181 gtaacaataa aagtcaacaa cgcgatgccc acgacggcag gcacgaacac cgccgccgcc
     3241 ttgtccgcca cgcgcgcaat cggtgcttta ctgccttgcg cttcagagag tgcgttcatc
     3301 atgtcgccga gcagggtttg gctgccgagc tgcgcggcgc ggtacaccac gctgccttca
     3361 gtcatcagcg cgcccgccaa tactttgccg cctgcctttt tctcttcggg attggattcg
     3421 ccggtaagat ggctttcgtc cgcccagccg ctgccgcttt ctatgatgcc gtcggcggca
     3481 atgcgttcgc cgtgattggc gcggattagg tcgccgattt gcacttggtc gatgggtagc
     3541 tgccgccatt cgccatcgcg ttgcacgttg acttgggttg gcgtgagttt gagcagcaag
     3601 cccaagctgt tcaggctgga ttttttggtg cggtgttcca aaaatttacc cagtgacaca
     3661 aaaccaatca ccattatgcc tacttcaaaa tacacatgcg ccataccgta cgccgcgtgc
     3721 gggctgaaaa acagcatata gacggaatac aggtaaatcg agaccgtgcc gatggtaacg
     3781 agtacgtcca tattcgccag cccgccttta atgctcgccc acgcgctttt gtaaaatggc
     3841 accgccagcc aaagctgcac cacactcgcc aatgcaaact gcaacaaggg cgacaacatc
     3901 caatcatgcc gtgtccaatt cagccctttt agcatcatcc ctaccatacc gataaggaac
     3961 gggatattga tggccagcaa aagccacaac ctccagccga tatggtgttc tgcttcgggt
     4021 tgcggcaatg tatcttccgt cttttccttc gcgccgtaac cggttttctc aatgattttg
     4081 gcaatgtcgg cagcggaagt tttgctgtcg tcaaacgtaa cctgcgcctc ctcgctggcg
     4141 aagttcactc ccgctgattt gacaaagtct tttttgttca acactttttc aatgcgcgaa
     4201 gcacatgcct gacaggtcat gccttcgatt tggaaacgga ttttttgttg cataagcgtg
     4261 attcctgctt tgatattcag acggcagtta aggctgcggg atgccgtctg agcgccggta
     4321 tggtcgaaat ttctcaaacg cgccgataat tcgagtagaa tccgaaaact tgacacagca
     4381 aaggaaaaac aatgtcccaa caccgcaaac tgattatttt gggttccggc cccgccggat
     4441 acaccgccgc cgtctatgcc gcgcgtgcca atttaaaccc cgtcattatt acaggtatcg
     4501 cgcaaggcgg gcaactgatg accaccaccg aagtggacaa ctggcctgcc gatgccgacg
     4561 gcgtgcaagg gccggaattg atggcgcggt ttctcgccca cgccgaacgt ttcggaacgg
     4621 aaatcatttt tgaccaaatc aacgccgtcg atctgcaaaa acgcccgttt acactcaaag
     4681 gcgatatggg cgagtacact tgcgatgccc tgattgtcgc aacaggcgcg tccgccaaat
     4741 acctcggttt gccgagtgag gaagcgtttg caggaaaagg cgtttccgct tgtgccacct
     4801 gcgacggttt cttctacaaa aaccaagatg ttgccgtagt tggcggcggc aatacggcag
     4861 ttgaagaggc actctacctt gccaatatcg ccaaaaccgt taccctgatc caccgccgca
     4921 gcgagttccg tgccgaaaaa atcatgatcg acaaactgat gaaacgcgtg gaagagggca
     4981 aaatcatcct caagctggaa agcaacctgc aagaagtact gggcgacgat cggggcgtaa
     5041 acggcgcatt attaaaaaac aacgacggtt ctgagcaaca aattgccgtc agcggcattt
     5101 ttatcgccat cgggcacaag ccgaataccg atattttcaa aggtcagttg gaaatggacg
     5161 aagccggtta cctgaaaacc aaaggcggca cggcggacaa tgtcggcgca accaatatcg
     5221 aaggcgtatg ggcggcgggc gacgtaaaag accataccta ccgtcaggcg attaccagcg
     5281 cggcttccgg ctgtcaggcg gctttggacg cggaacgctg gctgggcagc caaaacattt
     5341 gaatgccgtc tgaaaccgaa attttcggcg gaatgcaagt ttttcgcacc aaatccttga
     5401 ttccgtcggc aaaccccaat ataatgcccg ttctttgaac cttgccttct gctttcgcat
     5461 ggcagaaggc tgtttttacc atccataagg agataacatg cgtcattacg agatcgtgtt
     5521 tatcgttcat cctgatcaaa gcgagcaagt gcccgctatg gttgaacgtt acaaaaccat
     5581 gattgctgaa gcaaacggta agattcaccg tttggaagat tggggtcgcc gccaactggc
     5641 ttacccgatt aacaaaatcc ataaagccca ttacgttttg atgaacatcg aaaccactcc
     5701 cgaagtggtt gaagagctgg aaaccgcatt ccgcttcaat gatgccgtat tgcgccatct
     5761 aaccatcaaa accaaacacg ccgttaccga agcatcccct atgttgggtg gtgaaaaggc
     5821 taagaacctg ttgagcggtg cgtctgaaga agcggtcgcc caataattgg gattcaataa
     5881 tcttgtttcg cttgccgcgt taattgaaaa ggttttccct attcgatata cgcctgccgg
     5941 aatccctgtt ttagatatta ttttaaagca caaatcgtgg caggaggaaa acgggcagca
     6001 atgccttgtc caattggaaa ttccggcacg gattttaggc aggcaggcgg aagagtggca
     6061 gtatcggcaa ggtgtatatg ttcacgtcga aggtttttta gctcaaaaaa gcagacgttc
     6121 ccttatgccg atgctcagga tacaaaatat tcaagaatat aaaggttaaa cgacaatggc
     6181 tcgtcaatca ttcaaacgta gaaaattctg ccgtttcacg gctgaaaaaa tccaagaagt
     6241 cgattacaaa caagttgatt tgctgaaaga ctttatctct gaaaacggta aaatcattcc
     6301 tgcacgcatc acaggaacga aggcattcta ccaacgccaa ttggctgttg ccgtaaaacg
     6361 cgcacgcttc ctggctctgc tgccttacac cgaccaacac aaataatttt ggagattgaa
     6421 tcatgcaaat tattctgtta gaaaaaatcg gcggtctggg caacttgggc gacatcgtaa
     6481 ccgttaaaaa cggctacgcc cgcaactttc taattcccgc aggtaaggca aaacgtgcga
     6541 ccgaagcgaa tatgaaagag tttgaagcac gccgtgccga attggaagcc aaacaggctg
     6601 aaattttggc agatgcccga gtccgtcagg aaaaattgga cggtcaaacc gttaccgttg
     6661 ctcaaaaagc cggtgtggac ggccgcctgt tcggttccgt taccaatgcc gacattgctg
     6721 ctgcaatcgt tgctgccggc atcgaagccg tgaaagcaaa tgtacgtctg ccgaacggtc
     6781 ctctgaaagc cgttggcgag tacgaagtgg aagtggcttt gcacactgat gccgttgcta
     6841 aaattaccgt tgccgtcgtt gccgcaaccg agtaattgaa attatcagat gccgtctgaa
     6901 accctgccga ttgtcggaat gtggtttcag acggtatttg ttttgttttt cgtatattct
     6961 gcaaaaatcg ggatggttgt ttttatagtg gattaaattt aaaccagtac agcgtacctg
     7021 gcttcaatat actcaatagg ataggtctct tcctaaccga aaaaataatt gtcttttccc
     7081 ttgcaaataa ttgaaatcaa cagattacta aaacacagcc aaaatattca attgacaaaa
     7141 acttttctga ttttgagaaa tctgtgaaaa tcaggggatt atttattttg aaaatggtat
     7201 cgggcataaa atttggctgt gtttcaatat ggtgctgata acatactttg tttcaaaatg
     7261 ttcaatagga taacttcctg attgaccgaa aaataatggc cttttccctt gcaagcagtt
     7321 gaaatcaaca gattgtcaaa atacatccta aaaatttaat aacttatata tatgattgca
     7381 aaggttacgg cttgtatgcc ggtcattatg gcggaaatac cgtttgggag gcagccgcat
     7441 cgcgtggaat cggcacggca ggcgatgcgc cgatgccttc gggcgtgaag gcggggcaat
     7501 cggcagtttg gaaaaaggca atggaatctg tgggcagaag aagttaaggg taaaagtcgg
     7561 gcgcacggtc aggttgtctg tccttttgtc ggaacaggag actgggcagc tttgtcctga
     7621 ttaattgctt gtgcaaacgg taaaaaggtt ttttcgtgtg ttttaaagca ggttgtacag
     7681 catttttccg gcaatcaaaa gcaacataat gccgaaggat tttttgagtt tggcagaaga
     7741 aagtttgtgg gcggttttga caccgagcgg ggcaaaggca atggttgccg cgctgaggac
     7801 ggcgacggcg ggcaggtaaa ggaagcccag tgacccttcg ggcaatcctg caatattcag
     7861 gccgttgagc agatacgata ttgcgccgga gagtgcaatc ggccaggcaa ggccggatga
     7921 tgtgccgatg gctttatggg cggggaagcc gcagtggatt aagaagggga cggaaagtga
     7981 accgccgcct atgccgaccc agctcgacat tgtgccgaac agtgtggaaa ccgcagtcag
     8041 tccgggcagt ccgggcagcg ggcgggatgc cgtctgaggg tcggtatgca gtgttttgaa
     8101 tgcgacggcg gttaaaaaca ggatgaagaa aatttgaagc ccgaacgctg ggatatattt
     8161 tgcggagagt gcgccagcga atacgccgaa taccataccc ggcatcatcg taaatacggt
     8221 tttccagtcg accgcctgtt ttttgtgctg ccccagcata ctggaaaagg cggtgaagac
     8281 catgacggcg aaggatgtgc cgacggcgag gtgttgcgcg taaggatgtt gtgccaaacc
     8341 ctgcaaatca agcacccata aaacgacagg gacaatcagc gtgccgccgc ctacgccgaa
     8401 caggccggca ataaaacctg ccgcactgcc tacggcaagc aggattaaga taatgtccca
     8461 atgccacatt taatccgcct ttctgtattt tttccagaat cggcgtgccg ccataatgta
     8521 gccggacagg ctgtatccga gaaagaacag gaagaggacg agcgacggtt cccaagtgac
     8581 cagaagcagc agtaagacgg caagcagcat tccgacaaag gggacttgtc tgcggatgtt
     8641 gatttcttta aaactccaaa aagggatttg gacaatcatc gacaggccgg caaacagtgt
     8701 gatgcccaat gcccaccagt ggacggcggg gaatttttcg acgctgtggt tgacccaaat
     8761 cagcccgaca atcagcgcgg cggcagtcgg actgggcacg ccgataaacc agcgtttgtc
     8821 caccttgccg atgagcgtgt tgaacagcgc aaggcgcagg gcggcgcagg cgcagtagat
     8881 gaaggcgacg gaataaccga ttttgccgaa ctgccaaagc tgccatttgt aggcaatcag
     8941 agcgggagca acgccgaagc tgaccatatc ggcaaggctg tcgagctgct ccccgaacgc
     9001 gctttggctg ttggtcagcc gcgccacgcg cccgtccata ccgtccagca acatagagat
     9061 gaataccgcg atggcggcgg tttcataacg tccgtgcatg gattgggtga ttgcgtaaaa
     9121 tgcggaaaac agcgcggcga tggtaaagga gttgggcagc aggtagatac tgttttgacg
     9181 cagggagcgt tgcggggtgt tggtcggggt ttccatagct gaagtgtctc acggacgtgg
     9241 agaatcgata ggggattata acccaaacca ttatcgaaca atacgccctt taggatgaag
     9301 ccctcggagg cggtttgttt ttctgatggc ggatgctttg cggggcggtt tgactctgcc
     9361 tgccaccggg tgtgttgttt cggatttttg ccggatggat attgttgccg ggcatttctg
     9421 ggcttgccgg aaggtatcgg ggggagggca tcggaatatg cggcgcaccg ttcaaaccgg
     9481 ggcggcgttt ccctattttc agggggttgc aggtttcaac aaattgaaag atattttttg
     9541 catttccgac cgtatgctga ttttgaagcg ggataaatta taggacaagg tttttcgccc
     9601 gcggcaagga ttgcccgcaa tgccgtctga aaacaacggc ttgccgctaa ggcggcggta
     9661 tgcggatttg cgctgccgaa atcggtttgc catacccggc ggtgcaatct gcggggaggg
     9721 ataagccgat aaggcgggac ttatcctgtt ttttgtttat aatgcaacag aatttggacg
     9781 aaccgtcggg aatttatcat gatgaaaaag atactggccg tatcggcact atgcctgatg
     9841 actgcggcgg cacgggctgc cgatacttac ggctatctcg ccgtttggca gaatccgcag
     9901 aatgcaaacg atgttttgca ggttaaaacc acaaaagaag attcgacgaa aagcgaagcg
     9961 tttgccgagt tggaagcttt ctgcaaaggt caggacacgc ttgcgggcat tgccgaagac
    10021 gagccgaccg gatgccggtc ggtcgtgtcg ctgaacaata cctgtgtcgc gctggcatac
    10081 ccgaaagcct tgggcgcgat gcgcgttgaa aacgccgttg tgattacttc tccgcgtttt
    10141 acgagcgttt atcaggtcgc actcaaccag tgcatcaaaa aatacggcgc acagggacaa
    10201 tgcggcttgg aaacagtgta ttgcacgtct tcttcttatt acggggggaa ctgtgcgctc
    10261 tttgattcaa aatctcaaat aaaacgggaa atgccgtctg aaagatgttc agacggcatt
    10321 tctatatcga cggtcaggat tctttcggat cgggcagcag gctgttcaac ataatggcaa
    10381 gtacggcgca caagcccacg ccggcaaagc tgaagctgcc caatttgagc gtcatgccgc
    10441 cgatgcctgt ggtcagtacc gagctgacga tgaccaggtt tttcggcagc atcaaatcga
    10501 ctttggcatc aatcagggtt ttgatgccta aagaagcaat cgtgccgaac agcagcagca
    10561 taatgccgcc cattactggc atcggaatgg aagccaaaaa cgcattgaat ttgccgaaaa
    10621 acgccatgca gacggcaaaa accgccgccc aagtcatgat gacggggttg ctgtttttgg
    10681 taatcatcac cgcacccgtt acttcgccgt aggtcgtaac cggcgggccg ccgatcagac
    10741 ccgcaacgca tacgcccaaa ccgtcgcctg caagggtttt gtccaagccc gggtctttcg
    10801 tatagtcttt ccccgtcaca ttgccgattg ccatgatgcc gccgatgtgt tcgatggcgg
    10861 gggcgacggc aacgggcagc ataaacagtg cagcctgcca gttgacctga ggcgtttcaa
    10921 aatggggaac ggcgaaccag ggcgcgtgtg caatgcttgc cgtgtccacc agtcccatca
    10981 gcagtgccaa aacataaccc gaagcgacac cgatcaagat aggaatcagc ttcatcatcc
    11041 tgctgccgaa aaccgatacg atggcggtaa cggcaaaggt aaagccggaa agaatcagcg
    11101 aatcggtata gtcgatgacc tgtttgccgt ccgcctgacc cattgccatg ctgcttgccg
    11161 ccacggcaac agacagaccg atgaccatga tgacggggcc gatgacgaca ggaggcagca
    11221 gtttgtgtac cgccgccagt ccgcgccaac ggatcagcgc ggcaaacaca aaatacataa
    11281 agccggcggc aaacagtccg aacatggtgg aaggcagccc ccattcgccg acggagtaga
    11341 taatcggtgc gataaaggca aacgaagaac caagaaaaat cggcactttg cgtttggttg
    11401 tgatttggaa cagcagcgtt cccaagcctg cgcccaaaag cgcaagagcc ggattcagac
    11461 cggtcagcag gggaaccagc accattgcgc cgaatgccac aaataaaatc tgcgcgccgg
    11521 aaacggcaag tttcagttgg ttcataccat cttctttcaa tcggttcaaa cggcacggat
    11581 tatactggtt tgccgagggt ctgcgtaaaa atacatatgg cgggcaacag atatgccgtc
    11641 tgaaaggcaa tgtctgtctg ttgcaggatg aaagcaggag aaatggagta taatccgaca
    11701 ctttccatat tcaatccgca agccgtccgg cttccttatt tcagacggca ttttccggta
    11761 atacggcatg acagaaaaat cccataaaaa aacagcaaaa ggcagggcgg gaagcccgtc
    11821 cccaacgtcc gcacgcaaca agaaagccga caacggcgca cggggcaata aagtttccga
    11881 gcggctcaaa gcggtcaaag agttgcagaa aaccgaaacc aaaaaggcgc gtcccgaaca
    11941 tgtcgtcaac cttatcggcg acgcactgtg gctgatgggt ttggcggcaa ccttgtattt
    12001 ggcgatttcc ctgatcagtt tcgatatggg cgatccgtct tggtcgcaca gttcgccggt
    12061 tgtggaagat gtcgccaatt ggggcggact gttcggcgcg tatgttgccg atgtcggcta
    12121 ttatcttttc ggctggtcgt tctggtggtg gatagcggct gcctgcgtca tgctgtataa
    12181 aaattttcgt ctgcacgcaa aacagacgga aaacgaggca tacaaccaca aaatcgctgc
    12241 cgccgcgctg tttgtcctga cggtcttcag ccccgtcttg gagtattttg tgctgggcgg
    12301 aaaatatgcc gactccctgc ctgtcggagc aggcggtatg gtcggcatac gcgtcggcgc
    12361 agtgtttgcg tggctgctgg ggaaatcggg cagcctgctg attatcttgg ttgttctgct
    12421 gttgtcgttg tccctgctgg tgcagatttc atggctggaa tttttgaacg gtgcgggcag
    12481 ggcggttcaa aaccgcctga gtgccttatc cggcaaggtc atggctttgg gaaaacgccg
    12541 gccgaatacc aaaacagacg gtgtcgatac ccaaaataca cggcgcatgg taaaagaagc
    12601 caagaatatt acggccaaac ccgttgcctt gcccgaaggc agcagcagca accgcaaatc
    12661 cgtcgcggtt tccgtcgcgc cgccgcccaa aattcaggtt tctctgtttg aagatgacga
    12721 acctcggcag gcgggcgaat accacaagcc tacattgaac ctattgcgga ttcctgacag
    12781 cgaacccgtc agcatcaatc ccgccgaatt ggagcgcact gccgaactga tcgaatccaa
    12841 actggcagaa ttcggcatcg gcgtacaagt cgtatccgcc acatccggtc ccgtcatcac
    12901 gcgctacgaa atcgaacccg cgcaaggtgt taaaggcagc caaattgttg ccttgtcgaa
    12961 agatttggca cgctctatgt cgctgcagtc cgtgcgtatc gtcgaaacca tcgcaggtaa
    13021 aaacacgatg ggcatcgagt tgcccaacga caaacgccaa gacgtgatgt tgagcgaaat
    13081 cttgtcctcg cccgtgtttg ccggagccaa atccaagctg accgtcgcgc tgggcaaaga
    13141 cattgccggt acccccgttg tcggcgactt ggcgaaaatg ccgcaccttt tggtcgccgg
    13201 tatgactggt tcgggcaagt ccgtcggcgt gaacggcatg attatgtcta tgcttttcaa
    13261 agctacgccc gacgaagtcc gcttcattat gatagacccg aaaatgctcg agttgagcat
    13321 ttacgacggc attccgcacc tgctctgtcc cgtcgtgacc gatatgcgcg aagcagggca
    13381 ggcgttgaac tggtgcgtcg ccgaaatgga aaaacgctac cgcctgcttt cccacgccgg
    13441 tgtacgcaat ctggaaggct tcaaccaaaa agtcgaagcc gcaaaagcgg caggcaagcc
    13501 gctgctcaat ccgttcagtc tgaaccccga caaccccgag cctttggaaa aactgccgat
    13561 gattgtggtc gttatcgacg aacttgccga cctgatgatg accgaacgca aagccgtcga
    13621 gcagcaaatc gcccgtctcg cccaaaaagc gcgcgccgcc ggtatccata tgattgtcgc
    13681 cacccaacgt cccagtgtcg atgtcgttac cggcctgatt aaagccaaca tcccgacgcg
    13741 tatggcgttt accgtgcaaa gcaaaatcga cagccgtacc atcctcgacc aaatgggcgc
    13801 ggacgaactg ctcaaatatg gcgattcgct gttccttcag cccggcagtg ccgaaccgac
    13861 ccgcctgcaa ggcgcgtttg tttccgatga tgaagtccac cacgtcgtcg ctttcgtcaa
    13921 ggagcaggca ccggcaaatt atgtagaagg gctgcttaca ggcgaagcgg cacaggaaac
    13981 ggcaaatatc gtcagcccca atgcagacag tgacgaattg ttcgatcagg ctgtcgccta
    14041 tgttttggaa agcaaaaaaa cctccatttc gtctttgcag cggcagttgc gtatcggcta
    14101 taaccgcgcg gcaaacctga tggaggcact ggaaaatgca ggtgtcgttt ctccctccga
    14161 tcttaacggc agccgtaaaa ttttggcgca caaggaccat ttatagccca tatcgcaaaa
    14221 tgccgtctga acggtggaat tggcgtttca gacggcatat tatgtttcag gcgaaacatt
    14281 gtgatatact tgccagctaa aatttcccct ttgcggcaat gcggttcaaa tatcgtaccg
    14341 ttgcgctgtt tgcttcccca tgtagggaag aaagtttatc attttatcaa cacaacaaat
    14401 ttaagggctt atgatgagcg taactgttga aactttagaa aatctggaac gcaaagtagt
    14461 gttgtccctg ccttggtccg aaatcaacgc agaaaccgat aaaaaactga aacaaaccca
    14521 acgccgtgca aaaatcgacg gtttccgtcc gggtaaagca cctttaaaaa tgattgccca
    14581 aatgtacggt gcgagcgcac aaaacgacgt gatcaacgag ctggtgcaac gccgcttcca
    14641 cgatgttgcc gttgcccaag agttgaaagt ggcaggcttc ccccgttttg aaggcgttga
    14701 agaacaagac gataaagaat ctttcaaagt tgccgccatt tttgaagtgt tccccgaagt
    14761 cgttatcggc gatttgtctg cacaagaagt tgaaaaagta accgcttccg tcggtgatgc
    14821 cgaagtggac caaaccgtag aaatcctgcg caaacaacgc acccgcttca accatgtcga
    14881 acgcgaagcc cgaaacggcg accgcgtcat cattgacttt gaaggcaaaa tcgacggcga
    14941 accttttgcc ggcggcgcat ccaaaaacta cgccttcgta ttgggcgcaa gtcaaatgct
    15001 gcctgaattt gaagccggcg tagtcggcat gaaggcgggc gaaagtaaag acgttaccgt
    15061 caatttccct gaagactacc acggtaaaga cgttgccggt aaaactgccg tgttcaccat
    15121 tacgctgaac aacgtttccg aagcgactct gcctgaagtc gatgcagatt ttgcaaaagc
    15181 cttgggtatt gcggatggcg acgttgccaa aatgcgcgaa gaagtgaaga aaaacgtaag
    15241 ccgcgaagtg gaacgccgcg taaacgaaca aaccaaagaa tccgtaatga acgcgctgct
    15301 caaagccgta gagctgaaag cacctgttgc tttggtcaat gaagaagccg cacgcttggc
    15361 aaacgaaatg aaacaaaatt ttgttaacca aggtatggct gatgctgcca acttggatct
    15421 gcctttggat atgttcaaag aacaagccga acgccgcgtg tctttgggtc tgattttggc
    15481 caaactggtt gatgaaaaca aactggaacc gactgaggag caaatcaaag ccgttgtcgc
    15541 caacttcgca gaaagctacg aagatcctca agaagtaatc gactggtact acgcagagcc
    15601 ttcccgcctg caagccccga cttctttggc gatagaaagc aacgtcgttg atttcgtttt
    15661 gggtaaagcc aaagtaaacg aaaaagcttt gtcttttgac gaagtgatgg gcgcgcaagc
    15721 ctgattatcc tgaaaatgcc gtcttagggc agtttttgaa agcaccgaag ccgcttttaa
    15781 agcccgcttt ggtgctttta catcatgaaa ggagacgaaa tgtcttttga taactatctt
    15841 gtccctaccg ttatcgagca gagcggtcgc ggtgagcgtg cattcgatat ctattcccgg
    15901 cttttgaaag agcgcatcgt attcttggtc ggcccggtaa ccgacgagtc cgccaatctg
    15961 gtggttgccc aactgttgtt tttggaaagt gagaatccgg ataaggatat tttcttctat
    16021 attaactcgc cgggcggttc ggtaacggcc ggtatgtcga tttacgacac catgaatttt
    16081 atcaagcccg atgtatcgac tttgtgcttg gggcaggcag caagtatggg cgcgttctta
    16141 ttgtcggcag gcgaaaaagg caaacgtttc gccctgccca acagccggat tatgattcac
    16201 cagcctttaa tcagcggcgg cttgggcggt caggcatccg atattgaaat tcacgcacgc
    16261 gaactgttga aaatcaaaga aaaactcaac cgcctgatgg cgaaacattg cggccgcgat
    16321 ttggcagatt tggagcgcga caccgaccgt gataatttca tgtctgccga agaagcaaaa
    16381 gaatatggtt tgatcgacca agttttggaa aaccgcgctt ctttgcagct ttaaaccttg
    16441 tgaagtagac aaatgccgtc tgaaaacagt tcagacggca tttgtcttat agggactgta
    16501 ttgggatgat tttgttctgg ttgcgtttgg agccgttttc gccgtagttg gttttgacat
    16561 actcttcaac aatagccaaa aattccgttg caatgttgtc gcctttcagc gttactttgc
    16621 gttcaccatc tacataaaca ggcgcgacgg gtgtttcccc cgtaccgggc aggctgatgc
    16681 cgatgtcggc caatttgctt tctccgggcc cattgacaac gcagcccatt acggcaacgt
    16741 tcagggattc aaccccagga taaagggtac gccatataga cattttttgg cgcaggtaat
    16801 tttgaacatc ttgtgccagc tcttgaaata cggtactggt ggtacgcccg cagccggggc
    16861 aggcggtaac catcggcgta aacgaacgca atcccatagt ttgtaaaatc tcttgcccga
    16921 cgacgacctc ctgagtacgc gggctgccag gttccggagt cagtgaaatg cggatggtgt
    16981 cgccgattcc ttcttgaagc aagacggata atgccgccgt tgatgcgaca atgcctttgc
    17041 tgcccatacc ggcttcggtc aaacccaaat gcagcggata ggcgcaacgg ctgcccagtt
    17101 cgcggtaaac ctgaatcaaa tcctgaaccg cgctgacttt gcacgacagg atgattttgt
    17161 cttcgggcag tcccaataga acggcttttt cggcagattc caaagcggag acaatcagtg
    17221 cttccttcgt cacttcttcg ggcggtttcg gcgcggaaga agcgaggttg gcatccatca
    17281 tacgtttggc gaggctttga tccaaagaac cccagtttac gccgatgcgg acggctttat
    17341 cgttttcagc agcagtccga atcataaagg caaatttttc atcgcctttt acgcctttgc
    17401 cgacattgcc gggattgatg cggtatttgg acaatgcttt gccgcattct ggaaattccg
    17461 ccaacaggcg ttcgccgttg aagtggaaat cgccgataag cggtgtggca tagcccatat
    17521 cgtccaagcg gcggcggatt tcggcaactt tggacgcggc ttcggggctg ttgacggtaa
    17581 tacgcaccat ttcggatccg gcgtcgctca attccttaat ctgcaatgcg gtggcttttg
    17641 catcggcagt gtcggtgttg gtcatagatt ggataacgac gggtgcttct gaaccgacgg
    17701 taatatgatc gatgcggact tgatgcgtct tgcggcgttg gagtgtgttc atatggtttg
    17761 aatctgttta ttgaccgatg aggacggttt gcaattcttc cgagtagggg aaattcgcct
    17821 gcaattgtgc ttcatattcg tatgccgcct gtgcgttgcc gagggctttg gcaattttcc
    17881 agcctagcag caaatcatcg gcctgaagga cttctaccct gctttggtat tttttaaagt
    17941 agtaatcggc atcgcccaac tgcccggcca gcattttggt gcgcgccagt tctttaaatg
    18001 cgggtgggaa ctgcggctgg gcggcgaggg aacgtttcaa ataggcttcc gccaatccga
    18061 attgcccctg ttttgcgctg catatgcctt tattcaggtt ggcaatataa ggggtcgggt
    18121 aggtggggtc ggccagggct ttgtcgaaat atgccataga ttcggcaggg cggttgagcc
    18181 tgccgcacag gaaccagccg tagttgttgt tgatttcggc actgtcgggt ttgatggaga
    18241 gggcttgccg gaaactttcc tgcgccttgt cgttaacttt caggtattga tagatttcgg
    18301 cacggaccag ccaggcaagc tcgtttttag ggtctgattt caaggcgtct tcaatacttg
    18361 ccgtcgcctg acggtagtcc tgaccgcgca tatattccat tgccaactgg gttttgatat
    18421 tggaaacctg attggctttt tctgcccgcg aggggcggta ggaagtgctg cacgcgccca
    18481 aggcaaggac aagtaataaa gagattcgtt tggatggctt aaaaggcata attacccctg
    18541 ttgtccgatt aaaatctgct gccatttttg ttggcggcgc gttttatcct gaacctgccc
    18601 cgccaactgt ccgcaggcgg catcgatgtc gtcgccgcgc gtttttcgta cggtaacgac
    18661 aaatcctgcc tgctgcaaaa tatcgcggaa cacacggatg ttctcattgc tggagcgttc
    18721 gtatccggag tttgggaagg gattgaacgg aatcagattg aacttgcagg gaacatctgt
    18781 gaccagttcg atcagttcgc gcgcatgttg cgccttatcg tttattccgt ccaacatgac
    18841 gtattcgaaa gtgatgaaat ccctgggtgc tttgaccaga tagcgttggc atgcggccat
    18901 caattctttc aagggatatt ttttgttcaa cggtacgatt tggttgcgga cttcgtcatt
    18961 ggaagcgtgg agggaaaccg ccaaagccac cggcatgaca tcgcgcaacc tgtccatttg
    19021 gggaaccata cccgaagtgg aaacggttac gcggcggcgg ctcaaaccgt agccgtggtc
    19081 gtccagcatg atgcttaagg cggtaacgac attgtcgaag ttcgccatcg gctcgcccat
    19141 acccatcatc actacattgg aaattacgcg ttcgtttttc ggtgtaacac ccatcgcttt
    19201 gtttgcccac cacaattgcc cgatgatttc ggcagcagtc aaattgcggt tgaagccctg
    19261 ccggccggtc gaacaaaatg tacattccaa agcgcagccg acttgtgagg aaatgcagag
    19321 cgtgccgcga tccgattcgg ggatgaagac ggtttccacc ccgttgcccg taccgacatc
    19381 caaaagccat tttcgagtgc cgtctgaaga tttttgagac atcatcagct tgggaatttc
    19441 gatgcctgcc tgttcgttca gtttatggcg caacgatttt gccaaatcgg tcatttcgtt
    19501 aaaattttgc gcgccggatt ggtgcatcca acgcataacc tgtttggcac ggaagggttt
    19561 ttcgcccata tcggcaaaat gtcgggtcag cccttgaagg tcgtagttga gcagattggt
    19621 tttcatgtgt ttgtgttttt cttaaatcaa ggcttcaaat aaaaaattgc agggcaggca
    19681 agtttgattt gaagcctttt gcaacagagt cttcagacgg catggatata ttttatgccg
    19741 tctgaagagg agttgggcgg tgtattgtat caacgggggc agatttcggt ttggctgaaa
    19801 aagtaggcaa tttccaaagc ggcattttcc acgctgtcgg aaccgtgtac ggcattaatg
    19861 ctgaccgaag tggcaaagtc cgcgcgtatc gtgccttcgg cggcttcgga aggattagtt
    19921 gcacccatca gttcgcggtt tttcaggacg gcgttttcac cctctaatac ctgaatcata
    19981 accggaccgc cggtcataaa ttcaaccaat ccggcgtaga aggggcggtc tttatgaacc
    20041 gcataaaatt cttgcgcctc tttgagggta agctgcttca ttttggcggc aacgattttc
    20101 agaccgttct cctcaaagcg gctgtatatt ttgccgataa cgtttttgcc gacggcatcg
    20161 ggtttgatga tggagatggt acgttcaatc gccatgctat atccttattt ttacttaaga
    20221 agaatcaaat cggtattcta tcaaaaaaat aacttatccg cctgaagcct tgggagatag
    20281 ggtcaggtgc ggctgttcca aataaacttc ggactgcatt tcgaccatcc ggctggcggt
    20341 gcgggtaaat tcttcggcaa aatcgccttc cgtatagata tggttgacat ctaccgcgcc
    20401 ggtggcacac agtttgaccc ggaaatcgta gagtacgtca atcagccaag tcagccgccg
    20461 cgcctccgcc ttttcttgcg gtgagagttg ttccaaacct gaaataaaaa ccatttcata
    20521 atgttcggcc aaatacagat agtcggactg tgagcggggg ccgaagcaca gtgcgcggaa
    20581 atcaaaccat atggcacggc cggactcggc tttgtgggga atctcccgac catggatggt
    20641 gctgatgccg gggttcaaat cggtaatgcc tgtcatttct ttgaacagtt ttgccagttt
    20701 tgcctcattt tcttcattgg caggcgtaaa gaaaatctcg gcggggcgga gggtacgcag
    20761 tcggtagtct tcaccgccgt caacgtttaa gacggtcagg ctggactcga tgagcgcgat
    20821 tgtgggaaga aaactgctcc ggttttgacc ttgcgggtag agttcggaag gcgcgtagtt
    20881 tgaagtcgcc accaaaacaa cgccctcgtt aagcaggttt tccagcagac ggcctaaaat
    20941 cattgcatcc gcaatatcgc tgacatgaaa ttcgtcaaaa cacaatacgc gggtttcttt
    21001 ggcaatctcg gcggcaacgg atttcaacgg gttgctttcg cttttcaggg ttttcagcct
    21061 ctggtggatt tctgccataa aggcatgaaa gtggacgcgg cgtttgcggc ggtaagggag
    21121 gcagccgaaa aaagcgtcca tcagaaagct tttgccgcgt ccgaccccgc catagaaata
    21181 aagccctttg gggacttgcg gggaacgcaa actcctgcct aaaaaacggt ttcttttgcg
    21241 tttgaacatc atcaattcgg tccaaagccg atcgaggtgt tcgatggcgg ctgcctgcgc
    21301 gtcgtcgcgg atgaagttgg gcagttgtga ggcagcctga taccaagtca gcgggctgtg
    21361 attttcaaac ggcggggctt taaaaagttg gtctctatta ctcatattta accttgttat
    21421 acttcttgcg gcggagaaag aaaatgctta ccgtcggtat tataaatgat ttccttcaaa
    21481 cttggcgttt tccgtggaaa agaataatga cgcgcacaag ggggggtatg agcaaaggcg
    21541 gcgtttcaat atggtgcaga tatgagctca aatgcctttt atagt
//
LOCUS       DAWKOG010000018        21436 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.018, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000018 DAWKOG010000000
VERSION     DAWKOG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21436
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.018"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(64..2037)
                     /locus_tag="WJK66_000511"
     CDS             complement(64..2037)
                     /locus_tag="WJK66_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEZ7578267.1"
                     /translation="MHPIETPDKTFHDGDGVSELGTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVTTGNQEIGGQKTFTAQTQFPSGIHLSANQTNWNGGHK
                     AYIGADADNAHIVFGDNTLRLHGANNRISYNNHDIFHKANKPRFAEDIEGKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGIYSLPTAGGSSNLPVENTACHIQVIAGTKHGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPVGAIVSFPKAVRNPAGYLRADGTTF
                     AQNTFPDLYRALGNSNRLPDLSRTDIGITAWFPSDQIPTGWLAFDDIRTRVTKTAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAGNSLAVGTKQEDEIKRHVHKVFSHWVNHPHAA
                     ALGYEDHNERQRSSLVSTWTDENLSDNGFLTPRPDSKMATGGDENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETINAGALDAGTLAQNLQDKADRDHTHIAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSGRVTGDRRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRESGRTSTGPRNRRRRDGLLEALQD"
     gene            complement(2048..2608)
                     /locus_tag="WJK66_000512"
     CDS             complement(2048..2608)
                     /locus_tag="WJK66_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HEZ7578268.1"
                     /translation="MSYQDILRGLLPPVSYARNAPRVRAQAEIDGAALDAVVESAQSV
                     ADAVDPRSAGQMLADWERVLGLDGTGKNRQRRVLAVMAKLNETGGLSIPYFVRLAEAA
                     GYQIQIDEPQPFRAGVNRAGDRLAPQEIMWVWHVNVRGGNNRITRFRAGISAAGDRLT
                     DYSDAVIESLFNRLKPAHTAIRFTYR"
     gene            complement(2605..3123)
                     /locus_tag="WJK66_000513"
     CDS             complement(2605..3123)
                     /locus_tag="WJK66_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ7578269.1"
                     /translation="MPAIRRPPAGGNRYDYKNWALSVDGVTSAYVYPLRRGLGTVDIA
                     ITSADGVPSEETVRRVQAYIDEMRPVTAKNALVLKPTVTAVPVTVQVKLDGIDLDDAK
                     RRIRTALKEYFDTLIPGDGLTVSQIEAAISNVDGVIDRRLTAPTANRAADTVNRIEWF
                     KAGAINVTEMPS"
     gene            complement(3074..3307)
                     /locus_tag="WJK66_000514"
     CDS             complement(3074..3307)
                     /locus_tag="WJK66_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HEZ7578270.1"
                     /translation="MSLSKLAKKTAQTAKNIGETLRAAFRGKITLVVSSEPIQRVQLS
                     GLADETLQDLEHLQEYGFASHPPTARRRQPLRL"
     gene            complement(3304..4449)
                     /locus_tag="WJK66_000515"
     CDS             complement(3304..4449)
                     /locus_tag="WJK66_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ7578271.1"
                     /translation="MQNNSYGYAVSVRVGGKEHRHWERYDIDSDFLIPADSFDFVIGR
                     LGPEAAIPDLSGESCEVVIDGQIVMTGIIGSQRHGKSKGGRELSLSGRDLAGFLVDCS
                     APQLNVKGMTVLDAAKKLAAPWPQIKAVVLKAENNPALDKIDIEPGETVWQALTHIAN
                     SVGLHPWLEPDGTLVVGGADYSSPPVATLCWSRTDSRRNIERMDIEWDTDNRFSEVTF
                     LAQSHGRSGDSAKHDLKWVYKDPTMTLHRPKTVVVSDADNLAALQKQAKKQLADWRLE
                     GLTLTITVGGHKTRDGVLWQPGQRVHVIDDEHGIDAVFFLMGRRFMLSRMDGTQTELR
                     LKEDGIWTPDAYPKKAEAARKRKGKRKGVSHKGKKGGKKQAETAVFE"
     gene            complement(4439..5770)
                     /locus_tag="WJK66_000516"
     CDS             complement(4439..5770)
                     /locus_tag="WJK66_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HEZ7578272.1"
                     /translation="MSGWHTLLQDASYKGVGFDIEVVDESNGKALAEHARPFVQGIDL
                     EDMGMTGRQVQINAVFWGKGYAGRLKKLLDALEQPGGGVLVHPVWGRMHNMIAASWSY
                     RHEADYVDYAGIDITFREAAEAQEIFVFENAFLVELEALIADIDTYREAAIGFVDAVL
                     AVDAGVSALWGSALGIWSAASGTFGAVRRLFDLDKIAFPDRGGYSAAAFKNGSAKLFA
                     DISVMVDTGIRREAGLADNAMHHAGWSPRQRFDGAAAVADRAAAIPDNLLTGRFSDGL
                     QNRLNRLTAKQVQPVAQAVRLLSTSSLLSVATALIEAHGEEMTAPDLIEVNRAMRRRM
                     QAEIAALRAVQTAAAESGGLTANAVYTEAYQTAESLRAAAGRLNALVAAVINQKPPLI
                     VRQAPIDGTIHQIAHEFYGDIARAAELVRLNPHIHHPAFIKRGTLVNSYAK"
     gene            complement(5770..7941)
                     /locus_tag="WJK66_000517"
     CDS             complement(5770..7941)
                     /locus_tag="WJK66_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein F"
                     /protein_id="HEZ7578273.1"
                     /translation="MANGNMKLSLVLTARDDGARRLLADTQRQLDRTAKSRAQLERQS
                     HTYALTGIRSEKQIQREIMLTQAAFNRLARSGKASQNDLARAAVATRNRIRELNAELK
                     QGTGFADKMGKIGRFGAAAVAGGAAAYTVLKPAMDNRKQLDENINRVSRQAFIEDNSK
                     SAAWIATEGAQQIKDLALELVEKNGGTHDKALDLISGMMTTSLNFAQTKNEAQAAYAF
                     ALASEGSGEDTAKLIKTLKDGGMSGKDLQLGLEHVLQSGLDGTFEVRDMVRELPSLLP
                     AAQQAGMNGVGGLDYLLSLLQSAANKSGSPAEAATNVQNLLSKTLSPYTIGRLKKMAN
                     PNDPKKGVDWIGSVVQGKQNGENAVQVLSRLADAMLVKDKQYQDYKKRAAAGDKTAAE
                     QANMLKGALLAQLLPDLQAKQGLLAATDMTQIREYMASLAGVTLDNGKIAKNNEARML
                     SAAAQQEQQESLAMLRESLTGTLVDMETSFKKLAAEYPNATLALQALTTAATAASAAM
                     LLTAGGGKGAGFLKDVGSKALVWGKASAGGVAAGATAAGGKLLSWGKSAGSGLMNNPA
                     LVKRAGLLGMLLYSESLGDGTLPKGLRGTKTTPEMINRLKNNGIRFEPAPKREQARGG
                     VPQYLAAPSAQPTDKMLSPLFSTQTAAYQAAIQQQTAAYQAALAQDTAAVTTGLAQVQ
                     SAMASASQTINTNVSLNIDGRVIANEVSRYQVAMFGRGAGQ"
     gene            8096..8678
                     /locus_tag="WJK66_000518"
                     /pseudo
     CDS             8096..8678
                     /locus_tag="WJK66_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249360.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8722..8991)
                     /locus_tag="WJK66_000519"
     CDS             complement(8722..8991)
                     /locus_tag="WJK66_000519"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578274.1"
                     /translation="MPKTVRRYGEPRRAVGGGAVKRSYLTAHKSYRQAVILMAKAGIG
                     ADAVRDMCHAELSAWFEDILSSLGIKTPKEEGVIVSKRLRKPEGK"
     gene            complement(8931..9296)
                     /locus_tag="WJK66_000520"
     CDS             complement(8931..9296)
                     /locus_tag="WJK66_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578275.1"
                     /translation="MITVKLTHGLTYNGKVVSELRLKPLTVGGELAAFALIDDLPELP
                     ENATKAELLQRDVLETLTYWSQQIEAQGIPSDILTAQWLMENLSTEDYHTVMAAQEDL
                     CLKPSAATASPDAPSAAEQ"
     gene            complement(9300..9677)
                     /locus_tag="WJK66_000521"
     CDS             complement(9300..9677)
                     /locus_tag="WJK66_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ7578276.1"
                     /translation="MSDATYTGAVIMEVNGRDIEIVSIKPQTTTGRKPVKTMNRNGRV
                     NGYCDGVTEHKLSVTAAIPIDGTEIDWDNITKAKITIYPINDEGRRTSYLDCFTVDTS
                     EQYEVDNEARIDIEMIALHKIKE"
     gene            complement(9742..11151)
                     /locus_tag="WJK66_000522"
     CDS             complement(9742..11151)
                     /locus_tag="WJK66_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HEZ7578277.1"
                     /translation="MPHIDFDTIPGSIRVPGQYIEFNTRNAVQGLPQNPQKVLMVAPM
                     LTAGIQPALEPVQLFSDAEAADLFGQGSLAHLMVRQAFANNPYLDLTVIGIADHSAGV
                     QATATVTLSGTATAPGVVEITIGGKQVSTAVNTGETAATVAGRLKTAITAADVTVTAS
                     GSGAAVTLTAKHKGEIGNESGLTVSTGNTGLTYQANVFTGGAKNADIATALSKVAGKH
                     YHIICSPFSDDANAKALSNHITNVFNAIEQRGCIGVLGMSAALSTATTATGKINDGRI
                     TCAWYKGAVEPNGIIAAGYAAVLAFEEDPAKPLNTLEIKGLAVTPDAQWPWFAECNNA
                     LYNGLTPLTAVNNRVQIMRAVSTYTKSANNTDDPALLDITTIRTLDYVRRSVKERIAL
                     RFPRDKLSDRLLPKVKSEILDVLIKLDQAEIIENAEANKGKLVVARAQNDPNRVNAII
                     PADVVNGLHVFAGRIDLIL"
     gene            complement(11154..11351)
                     /locus_tag="WJK66_000523"
     CDS             complement(11154..11351)
                     /locus_tag="WJK66_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HEZ7578278.1"
                     /translation="MSKIKVTAADGLRVPTEHNPHEYIGQEPVEVDGNSLYYRRMIDD
                     GDLVVVEDAAPNTKTRNTKGE"
     gene            complement(11348..11998)
                     /locus_tag="WJK66_000524"
     CDS             complement(11348..11998)
                     /locus_tag="WJK66_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HEZ7578279.1"
                     /translation="MITDIEQAITDRLKRGLGRMVRTVKSYNGEADDLAGQIHTLPAV
                     WVTYGGSKVEPASTGGVCGRYQDTAEFVVMVAARNLRNEQAQRQGGIDSREIGSNDLI
                     RAVRRLLDGQRLGFADSRGLVPKAVRAIANHVLVQNAAVSIYAVEYAIRFNTCGLEND
                     RYPERTDNPDDPNHIFTKYQGTLSEPWPDFEGLDGKIYDPQSADEIPVNLTLKDKQ"
     gene            complement(11988..12410)
                     /locus_tag="WJK66_000525"
     CDS             complement(11988..12410)
                     /locus_tag="WJK66_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 family protein"
                     /protein_id="HEZ7578280.1"
                     /translation="MAYATVEDMVARFGELEVLQLTDRNNEGLIDREVAQTALVDATA
                     EIDAYLGRFRRPFEDLPPILVRLCCDIARYRLTAAQGVLITDEIRNRYKIDVLDLLRA
                     MAKGEVQLGVDDSGEEVAAGEDGIVFVNGKNKVFGRDH"
     gene            complement(12410..12808)
                     /locus_tag="WJK66_000526"
     CDS             complement(12410..12808)
                     /locus_tag="WJK66_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578281.1"
                     /translation="MAKTNNKPETAETAAPSFEDIKAELDAVQAELAAARNDVEMLTT
                     ALEKAEDDKKALSAELAELKVQHTQRAADALADSRDVMLVSTGADGKEFWRGGLLFDG
                     GWREVKRAEVGEAVWKAICAEPMLQRKAVE"
     gene            complement(12873..13781)
                     /locus_tag="WJK66_000527"
     CDS             complement(12873..13781)
                     /locus_tag="WJK66_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HEZ7578282.1"
                     /translation="MDKSAILTAITAAFRKEFQTGLESVKPDYTAVAMTIPSNTATNT
                     YAWLGKFPQMREWVGSRQIEKMNKQAMSLDNKKFEATVGVARTDIEDDQVGMYRPMMA
                     AMGESAAALPDTLVWGLLKKGKTTVGYDGQYFFDTDHPVYEKSDGTGQNTPHSNLTTG
                     TDNDAPTFYVVDDTKTLKPLIFQNRTDSEFETKFDPSKSDRVFMEDEYLYGSRRRCNA
                     GFGLWQLMHMAEKTALTRENLAAIIVKMQKIKADGGYVLNVKPSLLVVPPELEDKARE
                     LLEADKINGTTNTFKGRLKLHVCVHL"
     gene            complement(13781..14791)
                     /locus_tag="WJK66_000528"
     CDS             complement(13781..14791)
                     /locus_tag="WJK66_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578283.1"
                     /translation="MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPA
                     WYLTEENGHDVALLANSSRNQLVVDYEHQTLYKEKNGQPAPAAGWMRWLEFTPKGMFA
                     EVEWTDKAAAAIAAKEYRYISAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASA
                     QILKPETEQNPMKELLQQLFGLPDAGEEELKAALSALVEAKPKDVALSADVFAQLAEK
                     DSRIAALTAQTAKPDLTKYAPISVVQELQSKVAALTSGKLLPAQKEWAEGVLKQPGGL
                     AFLTGFIENAQPVAALAGSQTGGKAPDERVAALTAEEAAAAKMLGMSGEEFVKIKESE
                     GK"
     gene            complement(15022..15438)
                     /locus_tag="WJK66_000529"
     CDS             complement(15022..15438)
                     /locus_tag="WJK66_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HEZ7578284.1"
                     /translation="MVDVKIDNIFVVLNQIERLGNGIENRYLLMRRLSETMHTAVKLN
                     FRYAGRPKWLGLKYRDGKPLSDSGRLKDSFSTLSDNDTALVGTNIVYAAIHNFGGMAG
                     RNRKVRIPQREFLTLTDDDKQALMDDVQDYFSGLIP"
     gene            complement(15566..16891)
                     /locus_tag="WJK66_000530"
     CDS             complement(15566..16891)
                     /locus_tag="WJK66_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEZ7578285.1"
                     /translation="MPAPDLGFALSLPPKKAIEWLESKKVTAESYRNLTASEIAKVYT
                     IARMTDLDMLNDIKTSMVESAKSGQSFDDWRKGILNLLSNKGWLHPNGHNGKDIIDPA
                     TGEVFGSPRRLETIYRTNMQTAYNAGQYQGYMANIDARPYWMYDAVGDSRTRPAHSAI
                     DGLVYRYDDPFWATFYPPNGYNCRCSVIALSERDVERQGRIVGQSTSDNLVETHKIYN
                     KKGDTYLTLAYKAPDGSLYTTDRGFDYNAGRMNYRPDLDKYDRALAHQFAKAEMGGAD
                     FKTSFKQLEKEFYEVKQRLNIDGKPDKEQKIKIRNALSRQLKFAAGVLSKEMQELAGM
                     TRATVWLSDDTLVKQVDSREGQSFGTDLYAMLPDLIHEPEYIFYDKSRGKESFVLLGK
                     PLEGNEKYRLMAVLKYLPQYDSIFLDSFRRADEKEIVKSKKRYEMKKGY"
     gene            complement(16878..18446)
                     /locus_tag="WJK66_000531"
     CDS             complement(16878..18446)
                     /locus_tag="WJK66_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HEZ7578286.1"
                     /translation="MAKKNNKTKIQKPEAALQTDVAQITATGRVIAEHPSNFITPQKM
                     RALFEDAESGDIRAQHELFADIEERDSDIAANMGTRKRALLTLNWRVAPPRNATPEEE
                     KLSDQAYEMMDSLPTLEDLIMDLMDAVGHGFSALEVEWVFSDGLYLPRNFIHRPQSWF
                     KWDKDNGLLLRTRENPEGEALWPLGWVVHTQKSRSLQQARNGLFRTLSWLYMFKHYAV
                     HDFAEFLELYGMPIRIGKYGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAA
                     SGTMATSNPFLQMADWCEKSAARLILGQTLTSGADGKSSTNALGNIHNEVRRDLLVSD
                     AKQVAQTITSQIIGPFLQINYPNADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQ
                     IPESWVRDKLVIPDVQEGEAVLVRQVPDNPINRTALAALSAHTVPSKATGRHQEILDG
                     ALDDALVEPDFNSQLNPMVRQAVAALNACNSYEEADAALNALYPNLDNAKLRTYMQQA
                     LFISDILGQDHARA"
     gene            complement(18446..20068)
                     /gene="terL"
                     /locus_tag="WJK66_000532"
     CDS             complement(18446..20068)
                     /gene="terL"
                     /locus_tag="WJK66_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEZ7578287.1"
                     /translation="MKSKEFLKSLAEYAAQLRQIIEAEVDGFDASPKAIAARRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSELHAFLFSRLPEIIRSPKGENEAVGAPRGEGKSTQ
                     VTQLFTLWCIVTGQKHYAVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFPEVCGQGRV
                     WQAGTIVTANDVKVQVAGSGKKLRGLRHGPYRPDLTVLDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYGSVLNRTLNNPFWHGIKFKAMKRWPDRMDLW
                     DRWEELFRNDGETVAEAFYLANKDEMERGAQTSWAARGVLALMKIRARDGHATFDSEY
                     QNDPVSGEDAPFAKSMKFWNDLPSDLVYFGALDPSLGKAGASRDPSAIIIGGYQRETG
                     KLYVVEAQIKKRLPDLIIEDVIRLHRQYRCKLWFVETVQFQEFLKDELVKRSAARGIP
                     VPARAVKPVSDKLLRIETLQPHMANGLILLNESQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGAVANCVPIEWQSPTDNDFDDEIKSKWSR"
     gene            complement(20089..20595)
                     /locus_tag="WJK66_000533"
     CDS             complement(20089..20595)
                     /locus_tag="WJK66_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ7578288.1"
                     /translation="MAHPKEIREKLRRLYVSDGQTLEIAAMMCEIPIATARSWKRAAK
                     ESSDDWDKVRAAYTLAGGGIEDLSRSLLAGFLVQYQSTMTMLQDTSIEGLMPSERAKL
                     LTSLSDAFTKTVAANAKVMPETSKLATAIEVLELFGEVVRERYPQHLQAYVELVEPLG
                     VEIEKKYR"
     gene            complement(20597..20794)
                     /locus_tag="WJK66_000534"
     CDS             complement(20597..20794)
                     /locus_tag="WJK66_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578289.1"
                     /translation="MSDPILEVLARIEAKQDDMLANQARMDEELQQIKKDCKKSAAVY
                     GGLGGVIVTTGWELLRAKFGG"
     gene            complement(20791..21057)
                     /locus_tag="WJK66_000535"
     CDS             complement(20791..21057)
                     /locus_tag="WJK66_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578290.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKQDAKADSTNIAAALERIENKLEKVNEKLDRKADKS"
     gene            complement(21069..21397)
                     /locus_tag="WJK66_000536"
                     /pseudo
     CDS             complement(21069..21397)
                     /locus_tag="WJK66_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213615.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4339 a         6106 c         5579 g         5412 t
ORIGIN      
        1 atctctacag ccatctctac agccatctct acaaccatct ctacaaccca tctctacagc
       61 ccgctagtct tgcagtgcct cgagcaagcc gtctcggcgg cggcggtttc ggggtccggt
      121 tgatgttctt ccactctctc tttgcggttc ttcgttggtt tcaggtattt cggggcctat
      181 cggggagctg gcggcgtttc ccgaggcggc tttgccgatg gccatccagt cgcaactgcc
      241 ttcgtaccag ttggtaataa tggcggcggc gtggtttttt gcgcggataa acagccagtt
      301 gtgttgcctt cgatcgccgg taacgcgtcc cgaatggcgt tcggatacga agcatttgac
      361 gttgccgtcg gcaaaggcga cggggaagac gacttcgttt tcgatggggc cgccgcttct
      421 tgtaaacctg taactgccgg tctgtatcat tgtgccgtcg gggaatctga caatatccac
      481 gccgccgatg gtttggcgtg tgaattgcgc ggcaacggcg gtgctgattt tttcgtccag
      541 cccttggatt tgggcggcga tgtgggtgtg gtcgcggtcg gctttgtctt ggaggttttg
      601 cgccagcgtg cccgcgtcca gcgcgcctgc gttgatggtt tcgccgtggg actttatcca
      661 gaacacggct tcgcccaagg tgtcggcggc tttgatgcac agttttaaaa ccagggcttt
      721 ggggcggttt tcgtcgccgc ccgttgccat tttgctgtcc gggcgcgggg ttaaaaagcc
      781 gttgtcgctt aaattttcgt ccgtccaagt cgatacgagc gagcttctct gcctttcgtt
      841 gtggtcttcg taaccgaggg ctgccgcgtg cgggtggttt acccagtgtg aaaatacttt
      901 gtggacgtgc cgtttgattt cgtcttcctg cttcgttccg actgccaagc tgttgcctgc
      961 gttgcggata aagcggtctt ccgcctgcgg gacgttttgg atgctgccgt attttccggt
     1021 cagcaggcgg tacagctcgg gataagcggt tttggttacg cgcgtgcgga tgtcgtcaaa
     1081 cgccagccag ccggtcggga tttggtcgga cggaaaccac gcggtgatgc cgatgtcggt
     1141 acggcttaaa tcgggcaggc ggttgctgtt gcccaaggcg cggtaaaggt cggggaaggt
     1201 gttttgcgca aaggttgtgc cgtctgcccg caaatagcct gcggggtttc ggacggcttt
     1261 gggaaaggat acgatcgcgc cgacggggat gccgtctgaa ttgagttttt tccatgcgga
     1321 ccagttgtcg tttgcgctgc tcgtttggtg gcgttcgtac acgtcggacg tgtaggcggg
     1381 ataacccaac tgcctgcacc agccgtgttt cgtgccggcg atgacttgga tatggcaggc
     1441 ggtgttttca acgggcaggt tggagctgcc gcccgccgtc ggcagggaat agatgccgtc
     1501 tgttttgagg gtgttcaaat cgccccggaa tgtttcgact ttgaaattgc cgatgccgta
     1561 tccggacagt gtgttcggtt tgccttcgat gtcttcggca aaacgcggtt tgttggcttt
     1621 gtggaagatg tcgtggttgt tgtaggaaat gcggttgttt gcgccgtgca ggcggagggt
     1681 gttgtcgccg aagacgatgt gggcgttgtc ggcatccgcg ccgatgtagg ctttgtggcc
     1741 gccgttccag ttcgtctggt tggcggataa atggatgccg cttgggaact gggtttgggc
     1801 ggtaaaggtt ttttggccgc cgatttcttg gttgccggtg gtaactacag ccagtctatt
     1861 cagtgctgcc agtaattgat taggctgcga cttatccggc tggataccgg ccgcagtcag
     1921 cacggccagc agctcggatt gcacttggtt gagccaccac gcgggcagga tggtgcccaa
     1981 ttcggacacg ccgtcgccgt cgtggaaggt cttgtcgggg gtttcgatgg ggtgcatggt
     2041 ttagtcctta tcggtaggta aatcggatag cggtgtgggc gggcttgagg cggttgaaca
     2101 ggctctcgat cacggcatcg ctgtaatcgg tcagcctgtc gcccgccgcc gagataccgg
     2161 cgcggaatcg ggtaatgcgg ttgttgccgc cgcgcacgtt aacgtgccac acccacatga
     2221 tttcctgcgg cgcaagacgg tcgcccgcac ggttgacacc ggcgcggaac ggctgcggtt
     2281 cgtcgatttg gatttgatag cccgccgcct cggccaaacg cacaaaataa ggaatactca
     2341 agccgcctgt ttcgtttagc ttggccatga cggccaacac acggcgctgg cggtttttgc
     2401 ccgtaccgtc caaacctaat acgcgctccc aatcggccag catttggccg gcgctgcgcg
     2461 ggtcgacggc atcggcaacg ctttgagccg attccactac cgcatccagc gctgcgccgt
     2521 ctatttctgc ctgcgcccgc acacgcgggg cattgcgggc atacgacacg gggggcaaca
     2581 gaccccgcaa gatgtcttga tagctcatga cggcatctcc gttacattaa tcgcgcccgc
     2641 tttaaaccac tcgatgcggt taaccgtatc ggcggcacgg ttggccgtcg gcgcagtcag
     2701 acggcggtcg atcacaccat ccacattgct gatagccgcc tcgatttgcg acacagtcag
     2761 gccgtcgccg gggatcaggg tgtcgaaata ttcttttaga gccgtccgta tgcggcgctt
     2821 ggcatcgtcc aagtcgatgc cgtcgagctt gacttgcacg gtaacaggca ccgccgttac
     2881 ggttggcttg agtaccagcg cattttttgc cgttaccggg cgcatctcgt cgatataagc
     2941 ctgtacgcgg cgcacagttt cttccgacgg cacaccgtcg gcggaggtaa tggcaatatc
     3001 caccgtaccc aagccgcggc gcagcggata aacatatgcg ctggttacgc cgtcaacact
     3061 caacgcccag tttttatagt cgtaacggtt gccgcctgcg ggcggtcggc ggatggctgg
     3121 caaagccgta ttcctgcaaa tgttcaaggt cttgcagggt ttcgtcggcc aagccgctca
     3181 actgcacgcg ctgtatcggc tcggacgaca ccaccagcgt gatttttccc cgaaaggccg
     3241 cgcgcagggt ttcgccgata tttttagcag tttgtgccgt ttttttcgcc aatttactca
     3301 aactcattca aacaccgccg tttctgcttg ttttttgccg ccttttttgc ccttatggct
     3361 cacgcctttg cgtttgcctt tgcgcttgcg cgccgcctcg gcctttttgg ggtaagcgtc
     3421 gggtgtccaa ataccgtcct ctttgagccg cagctcggtt tgcgtgccat ccatgcggga
     3481 tagcataaac cgccgcccca tcagaaaaaa caccgcatcg ataccgtgct cgtcgtcgat
     3541 cacatgcaca cgctggccag gttgccacaa tacgccgtcg cgggttttat ggccgcccac
     3601 ggttatcgtg agtgtcaatc cctccagccg ccagtcggcc agctgctttt tagcctgctt
     3661 ttgcaatgcg gccaaattgt cggcatcgga caccaccacc gttttagggc ggtgcagcgt
     3721 catcgtcggg tctttgtaca cccactttaa atcgtgtttg gcgctgtcgc cgctgcggcc
     3781 gtgcgattgc gccaaaaaag taacctcgga aaagcggttg tcggtatccc actcaatgtc
     3841 catgcgctcg atattgcggc ggctgtcggt gcggctccaa cacaatgtcg ccaccggcgg
     3901 gctgctgtaa tccgcaccgc ccaccaccaa cgtgccgtcc ggctccagcc acggatgcag
     3961 cccgaccgag ttggcaatat gggttaatgc ctgccatacg gtttcgcccg gctcgatgtc
     4021 gattttgtcc aaagcggggt tgttttcggc cttaagcacc accgctttaa tctgcggcca
     4081 cggcgcggcc agctttttgg ctgcatccaa taccgtcatg ccctttacat tgagctgcgg
     4141 cgcggagcaa tccaccaaaa aaccggccaa atcacgcccg ctcaagctca actcgcggcc
     4201 gcccttgctt ttgccgtggc gctggctgcc gatgatgccc gtcatcacga tttgcccgtc
     4261 tatcactacc tcgcagctct ctccgcttaa atcgggtatg gccgcctccg gccccaacct
     4321 gccgatgaca aaatcgaagc tgtcggcagg gattaaaaag tcgctgtcga tgtcgtagcg
     4381 ctcccagtgg cggtgctctt taccgcccac gcgcaccgac acggcatagc cgtatgaatt
     4441 attttgcata gctgttgacc aaagtgccgc gcttgataaa cgcggggtgg tggatatggg
     4501 gattgagccg caccagctct gctgcgcggg ctatatcgcc gtaaaactcg tgggcgattt
     4561 ggtgtatcgt accgtcgatt ggggcttggc gcacaatcag cggcggcttt tggttgatga
     4621 ccgccgcaac caacgcattc agacggcctg ccgccgcgcg cagggattct gccgtttggt
     4681 aagcctcggt atacacggcg ttggccgtca gcccaccaga ctcggcagca gccgtctgca
     4741 ccgcccgtaa ggcggcaatc tcggcctgca tacggcggcg catggcgcgg ttaacctcaa
     4801 tcaaatcggg cgcggtcatc tcttcgccat gcgcctcgat taatgccgtt gccaccgaca
     4861 acagcgatga cgtggataac aggcgcaccg cctgcgctac cggctgcacc tgtttggcgg
     4921 ttaaacggtt caggcggttt tgcaggccgt ctgaaaagcg gccggtcagc aaattatcag
     4981 ggatagcggc ggcacggtcg gcaacagccg cagccccgtc aaaccgctgt cgcggcgacc
     5041 aaccggcatg gtgcatggca ttatcggcca aacccgcctc acggcgtatg ccagtatcta
     5101 ccatgaccga tatatccgca aacagcttgg ccgagccgtt tttaaacgcc gctgcgctgt
     5161 atccgccccg atcgggaaag gcaattttgt ccaaatcaaa caaacggcgc accgcaccaa
     5221 acgtacccga tgccgcactc caaatgccca gcgcgctgcc ccataaagcc gatacgcccg
     5281 catccaccgc caacaccgca tcaacaaagc cgatagccgc ctcgcggtag gtgtcgatat
     5341 cagcaatcaa cgcctcaagc tcgaccaaaa aggcgttttc aaaaacaaag atttcctgcg
     5401 cttcggccgc ctcgcggaaa gtaatatcga tgcccgcata atccacataa tcggcctcat
     5461 gtcggtaact ccatgatgcc gcaatcatgt tgtgcatccg cccccaaaca gggtgcacca
     5521 gcacgccgcc gcccggctgc tccagcgcat ccagcagctt tttcagacgg cctgcatagc
     5581 ccttgcccca aaacaccgca ttaatctgca cctgccgccc ggtcatgccc atgtcttcaa
     5641 ggtcgatacc ctgcacaaac ggccgcgcat gctcggccaa tgccttgccg ttgctctcgt
     5701 ccaccacctc aatatcaaag ccgacgccct tgtaagatgc gtcctgcaat aaggtatgcc
     5761 atccgctcat tattgacccg ctccacggcc gaacatggcc acttgatacc gcgatacctc
     5821 attcgcgata acacgtccgt cgatattcag gctcacattg gtattgatgg tctgacttgc
     5881 cgacgccatc gcactttgca cttgtgccaa acctgttgta actgcagccg tatcctgcgc
     5941 caatgctgcc tgatacgccg ccgtctgctg ctgaatggct gcctgatacg ccgccgtctg
     6001 agttgaaaac aacggagaca acatcttatc ggtaggctgc gctgacggag cagccaaata
     6061 ctgagggaca ccaccccgcg cctgttcccg cttcggcgca ggttcaaatc ggataccgtt
     6121 gtttttcaga cgattaatca tttcaggagt tgtcttggta ccacgcaaac cctttggcaa
     6181 tgtgccgtca cccaaagact cggaatacag caacatacct aacaaacccg cccgtttaac
     6241 taacgctgga ttattcatga gcccgctacc ggcagatttt ccccatgaca gcaacttacc
     6301 gcctgccgct gtggcacctg ctgccacgcc gcctgcggaa gccttacccc ataccaacgc
     6361 tttactacct acatctttca gaaagcctgc acctttaccg ccaccggcgg ttaataacat
     6421 tgcggcagac gccgctgttg ccgccgtcgt caatgcttgc agggctagag tggcattagg
     6481 gtattccgct gccagctttt taaacgaggt ttccatatcc accaatgttc ccgtcagact
     6541 ttcccgcaac attgccagcg attcctgttg ctcttgttgc gccgctgccg acaacattcg
     6601 cgcctcgttg ttcttagcaa tttttccgtt atccaacgtt acgccagcca acgaagccat
     6661 atattcacgg atttgcgtca tatccgttgc agccagcaaa ccttgttttg cctgcaaatc
     6721 aggcagcagt tgcgccaaaa gcgcgccctt aagcatattt gcctgctccg ccgccgtctt
     6781 atcgcctgca gccgcgcgtt tcttataatc ttggtattgc ttatccttta ctagcatggc
     6841 atcggcaaga cgggacaaca cctgcactgc gttttcgccg ttttgcttgc cttgcacaac
     6901 cgagcctatc caatcgacac ctttcttcgg gtcatttgga tttgccatct tcttcagacg
     6961 acctatcgtg taaggcgaca gagttttact caaaagattt tgcacattag tcgccgcttc
     7021 ggcaggactg cccgatttat tcgccgcaga ttgtaagagt gagagcaggt agtccaaacc
     7081 gccgacacca ttcatccctg cctgttgcgc ggcagggagc agactcggca gctcccgaac
     7141 catatcccgc acctcgaaag tgccgtctaa acccgattgc aagacgtgct caagcccgag
     7201 ttgcaggtct ttaccgctca tgccgccatc tttcagggtt ttaatcagtt ttgccgtatc
     7261 ctcgccactg ccttctgagg caagtgcaaa agcatatgcc gcctgcgctt cattcttggt
     7321 ttgggcaaaa ttcagactgg tggtcatcat gccgctgatt aaatccaaag ccttatcgtg
     7381 ggtcccgcca tttttctcga caagttcaag tgccaaatcc ttgatctgtt gcgcaccttc
     7441 agttgcaatc cacgctgccg atttactgtt atcctcaata aatgcctgtc tggacacgcg
     7501 gttgatgttc tcatcaagct gctttctgtt gtccatagca ggcttaagca ccgtatacgc
     7561 tgccgcgcca ccagccaccg cagctgcacc gaatcttccg atttttccca tcttgtccgc
     7621 aaatcccgtg ccctgtttca gttccgcgtt cagctcgcga attcggttac gcgtagcgac
     7681 cgccgcccgt gccaaatcat tttgtgatgc cttgccgctg cgcgccaaac ggttaaacgc
     7741 agcctgtgtc agcatgattt cgcgttgaat ctgtttttct gagcggatgc cggtcaacgc
     7801 ataagtatgg ctttgccgtt caagttgcgc ccgcgatttc gcggtacgat ctaattgtcg
     7861 ttgagtatca gccagtagcc gtctcgctcc gtcatctcgg gcggttaaca ccaacgacag
     7921 tttcatgttc ccgtttgcca taaaaagcag cctttaaacg tattaactca aggtcaatta
     7981 tcgtttaaag gctgtttaag ggaggcggta tgggcttcaa ccccgttttc gtgctttatt
     8041 tgtttgttgc gttataatac cagaatgata accattttat tcaaagggat attttatgaa
     8101 tatgaaaaaa ttgatttccg caatttgtgt ttcaattgtt ttatcagcct gcaaccaaca
     8161 atcaaaaacg gcacaagccg aagaacctgt ccaaagtatc caggctgctg attgtaccgc
     8221 cccaatggac atcacagttg aacaatatct catcaatttg gagcaagcat ttaaaactca
     8281 gaacgtctca acaaaaatcc ataataaaaa tattgtcaag accgattgtg gttatgacct
     8341 tactttggta atggattttg gggcgattgc gctcaaactg gacgagcagc aaaaaattag
     8401 agctatctca gtaggctaca ttttaaaaac cgacggagag aaaggacaaa atctagtcaa
     8461 taatgccata aatggattac acagtattca ggcagttctg tctttaacta ccacagacaa
     8521 attgggcgaa tcggaagcag gaaaacaact tcgtcaaaga atccaatcag acaggagcaa
     8581 cagcgcaaaa agacgttccg gcagatggta ttttatatag cgttgttttt gaaaaagaaa
     8641 caaacaccat tgcaataatc ggcagaaaac aaccctaacc gtcaaataaa aaaaaggtcg
     8701 tctgaaactc agacgacctt tttattttcc ctccggcttt ctcagccgtt tcgacacaat
     8761 cactccctct tccttcggcg tttttatgcc caggctcgac aggatgtctt caaaccatgc
     8821 cgacagctcg gcgtggcaca tatcgcgcac tgcgtccgcc cctatccccg cctttgccat
     8881 caggatgacc gcctgacggt agcttttgtg tgccgtcagg tagctgcgtt ttactgctcc
     8941 gccgccgacg gcgcgtcggg gctcgccgta gcggcggacg gttttaggca caaatcctcc
     9001 tgagccgcca tcacggtatg gtagtcttcg gtagagaggt tttccatcag ccactgcgcc
     9061 gtcaggatgt cggatgggat accttgggct tcaatctgct gcgaccagta ggtcagcgtc
     9121 tctaggacgt cgcgttgcag cagttcggct tttgtggcgt tttcgggcag ctcgggcaag
     9181 tcatcaatca gggcgaacgc cgccagttcg ccgccgacgg tcagtggctt gaggcgtaat
     9241 tcagatacga ctttgccatt gtaggtcagc ccgtgggtca gtttgacggt aatcacgcat
     9301 tactccttga ttttgtgcaa agcaatcatc tcaatgtcga tgcgtgcctc gttatcgact
     9361 tcatattgct cgctggtatc gacggtaaag cagtcgaggt aggaagtgcg gcgaccttcg
     9421 tcgttgatgg ggtaaatcgt gattttcgcc ttggtgatgt tgtcccagtc gatttccgta
     9481 ccgtcgatcg gaatggcggc ggtaacgctt aatttgtgtt cggttacgcc gtcacaataa
     9541 ccgttgactc ggccgttgcg gttcatcgtt ttgaccggct tgcggcctgt agtggtttgc
     9601 ggtttgatgc tcacaatctc gatatcgcgg ccgttcacct ccataatcac cgcgccggta
     9661 taggtagcgt cgctcatgct ttatcctttt ttaaggtttc agacggcctt taaacctgtt
     9721 ttaaacgccg tctgaaaagg gttacaaaat caaatcaatg cgcccggcaa agacgtgcag
     9781 gccgttgacc acatcggcgg ggataatggc attaacacgg ttggggtcgt tttgcgcacg
     9841 cgccaccacc agcttgcctt tgttggcctc ggcgttttcg atgatttcgg cttggtcgag
     9901 cttaatcagc acgtccaaaa tctcgctctt aaccttgggc agcaggcggt cgctcaattt
     9961 gtcgcgcgga aaacgcaggg caatgcgctc tttaacgctg cggcgcacat aatccagcgt
    10021 gcgaatggtg gtaatgtcga gtagtgccgg gtcgtcggtg ttgttggccg acttggtata
    10081 ggttgatacg gcacgcataa tctgcacgcg gttgttgacc gctgtgagcg gggtcaagcc
    10141 gttgtacagc gcattgttgc attctgcaaa ccacggccat tgcgcatcag gtgtaacggc
    10201 cagccctttg atttccagcg tgttcagcgg cttggcaggg tcttcttcaa aggccaacac
    10261 cgccgcataa cctgcggcga tgatgccgtt tggctctacc gcacctttgt accaagcaca
    10321 ggtaatgcgg ccgtcgttga ttttgccggt agcggtggtg gccgtgctca aggccgcact
    10381 catacccaat acgccgatac agccgcgctg ctcgatggcg ttgaatacgt tggtaatatg
    10441 gttgctcaag gctttggcgt tggcgtcatc gctaaacggg ctgcaaataa tgtgataatg
    10501 cttgcccgcc actttggaca aggccgtggc aatgtccgca tttttggcac cgccggtaaa
    10561 gacattggct tgataagtca ggccggtatt gccggtgctc acggttaagc cgctctcgtt
    10621 gccgatctcg cctttgtgtt tggccgtcag cgtaacggct gcgccgctgc cggatgcggt
    10681 aacggttaca tcggcggcag tgatggcggt tttcagacgg cctgccactg tggcggcggt
    10741 ctcgccggtg ttaacggccg tgcttacctg cttgccgcca atcgtgattt ccaccacgcc
    10801 cggcgcggtg gccgtgccgg aaagggtaac ggttgcggtt gcctgcacgc ctgcgctgtg
    10861 gtcggcaata ccgataacgg tcaaatccaa ataagggttg ttggcaaatg cttggcgcac
    10921 catcaaatgc gccagcgagc cttgtccgaa caaatcggcc gcctcggcat cgctaaatag
    10981 ttgcaccggc tctaaggcgg gctgtatgcc cgcggtcagc atgggtgcaa ccatcaatac
    11041 cttttgcgga ttttgcggca aaccttgcac ggcattgcgg gtgttaaatt caatatactg
    11101 cccgggcacg cggatgctgc ccggaatcgt gtcaaaatca atatggggca tcattactct
    11161 cccttagtat tgcgggtttt ggtatttggg gcggcatcct caaccaccac caaatcgccg
    11221 tcatcaatca tgcggcggta atacaggctg ttgccgtcca cctccaccgg ctcttggccg
    11281 atatattcgt gcgggttgtg ttcggtcggc acacgcaggc catctgccgc cgttactttg
    11341 attttgctca ttgcttatcc ttaagggtta ggtttacagg tatttcatcg gcggattgcg
    11401 ggtcgtaaat tttgccgtcc aacccctcga aatcaggcca cggctcgctc aatgtaccct
    11461 gatacttggt aaagatatgg ttggggtcgt cgggattgtc ggtgcgttcg gggtagcggt
    11521 cattttccaa cccgcaggtg ttaaagcgga tggcatactc aaccgcatat atgcttactg
    11581 cggcgttttg caccagcaca tgattggcaa tcgcgcgcac cgctttgggc accaagccgc
    11641 ggctatcggc aaaaccgagc cgctggccgt caagcaggcg gcgaacagcg cggattaaat
    11701 cgttgctgcc gatttcgcgg ctgtcgatgc cgccttgccg ctgcgcctgc tcgttgcgca
    11761 gattgcgggc cgccaccatc accacaaatt cggcggtatc ctgataacgt ccgcatacgc
    11821 cgccggtgct ggcaggctca actttgctgc cgccatacgt tacccaaacc gcaggcagcg
    11881 tatggatttg ccccgccaaa tcgtcggcct cgccgttgta gcttttaacc gtgcgcacca
    11941 tgcgacccaa gccccgtttc agacggtctg ttatcgcttg ctcaatatca gtgatcacgc
    12001 ccgaacacct tatttttacc gtttacaaac acaataccgt cttcgcccgc ggccacttct
    12061 tcgccgctat catccacgcc cagctgcact tcgcctttgg ccatagcacg cagcaggtcg
    12121 agcacgtcga ttttgtagcg gttgcggatt tcgtcggtaa tcaacacgcc ctgagccgcc
    12181 gtcagacggt agcgggcaat gtcgcagcaa aggcgcacca agatgggcgg cagatcctca
    12241 aaaggtcgtc tgaaccgccc cagatacgcg tcgatttcgg cagtggcgtc caccagcgcg
    12301 gtttgtgcga cctcgcggtc aatcagcccc tcgttgttgc ggtcggtgag ctgcaagact
    12361 tccagctcac cgaaacgcgc aaccatatcc tcaaccgtcg cgtatgccat tactcgaccg
    12421 ccttgcgttg cagcataggc tcggcgcaga ttgccttcca caccgcttcg ccgacttcgg
    12481 cgcgcttcac ttcgcgccag ccgccgtcaa acagcaggcc gccgcgccaa aattctttgc
    12541 cgtctgcgcc ggtactgacg agcatcacat cgcggctgtc cgccaaagcg tcggcggcgc
    12601 gttgcgtatg ctgcactttg agttcggcaa gttcggcgga cagtgccttt ttgtcgtctt
    12661 cggctttttc caaggctgtg gtcagcattt cgacatcgtt tcgggcggcg gcaagctctg
    12721 cctgcacggc gtccaattcg gctttgatgt cttcaaacga cggggcggcg gtttcggcgg
    12781 tttctggttt gttgttggtt tttgccatga tgactccttg tttcagacgg cctttaaata
    12841 ggtgttaaag gtcgtctgaa aacggttcgg ggttacaggt ggacgcaaac gtgcagtttc
    12901 agacggcctt tgaaggtatt ggtcgtgccg ttgattttgt cggcttccaa taattcacgg
    12961 gctttgtctt ccagctcggg cggaacgacc aagaggctgg gcttcacatt caacacatag
    13021 ccaccgtcgg ctttgatttt ctgcattttg acgataatgg cggcgaggtt ttcgcgggtc
    13081 agcgcggttt tttccgccat gtgcatcagt tgccacaaac cgaaaccggc attgcagcgg
    13141 cggcgcgatc cgtacaggta ttcgtcttcc ataaacacgc ggtcggattt ggacgggtcg
    13201 aatttggttt caaactcgga gtcggtgcgg ttttggaaaa tcagcggctt cagggtcttg
    13261 gtgtcatcga cgacgtagaa ggtcggcgcg tcgttgtccg taccggtcgt cagattggag
    13321 tgcggcgtat tctgacccgt gccgtccgat ttctcgtaaa cggggtggtc ggtatcgaag
    13381 aaatattggc cgtcataacc gacagtggtt ttaccttttt tgagcagacc ccacaccagc
    13441 gtatcgggca gggcggcggc ggactcgccc attgccgcca tcatcgggcg gtacatgccg
    13501 acttggtcgt cttcgatgtc cgtacgcgct acgccgacgg ttgcttcaaa ctttttgttg
    13561 tcgaggctca tcgcctgctt gttcattttt tcgatttggc gcgagccgac ccattcgcgc
    13621 atttggggga atttgccgag ccacgcataa gtattggttg ccgtattgga cggaatggtc
    13681 atcgcaacgg cggtatagtc gggcttgacg gattccaaac ccgtttgaaa ttctttgcgg
    13741 aatgctgccg tgattgcggt caaaatcgct gatttgtcca ttacttacct tcgctttctt
    13801 tgatttttac aaattcttcg ccggacatgc ccagcatttt tgctgcggct gcctcttccg
    13861 cagtcagtgc ggcgacgcgt tcgtcgggtg ctttaccgcc cgtttgcgag cctgccagtg
    13921 cagcgaccgg ctgggcgttt tcaataaagc cggtcaaaaa tgccaagccg cccggctgtt
    13981 tcaatacgcc ttctgcccac tccttctgag caggcagcaa tttgcctgaa gtcagcgcgg
    14041 cgactttgct ttgcagctct tgaaccactg agataggcgc gtatttagtc aaatcaggct
    14101 tggcggtttg cgccgtcaat gccgcgatgc ggctgtcttt ttccgccagc tgcgcgaaca
    14161 cgtcggcaga caatgccacg tctttcggct tggcttccac gagcgcggac aatgccgcct
    14221 tcagttcttc ttcgcccgca tcaggcagac cgaacagttg ctgtaacaac tctttcatag
    14281 ggttttgctc cgtttccggt tttaaaattt gcgccgacgc tgccgccagc acctcgtcca
    14341 taccgtccaa cgcggggaaa tttgtcagcg cggcgtgaaa aattttgctt acatatccct
    14401 ttgtgtcata ggaaaacaca gcagagatgt agcgatactc ttttgcggca attgccgcag
    14461 ccgccttgtc cgtccactcc acttcggcaa acatgccttt aggcgtgaac tccagccaac
    14521 gcatccaacc ggcggcaggt gcaggttgtc cgtttttctc tttgtagagc gtctggtgtt
    14581 cataatcgac aaccaactga ttgcgcgagc tgttggccaa caacgcgaca tcatgaccgt
    14641 tttcttcggt cagataccac gcagggacat cagtcggacg accgtctact gcgcgaaatt
    14701 cgccatatgg cagcagttgg attcgcccgt cttttggctg cacctcgaaa ctgcacacgg
    14761 caagtagggt tttttgtgca tttttggaca tgtgtacctc tctcaatacg gtcattttgc
    14821 ctatttgaaa aaacaacaaa gagtaggcgt gcttcaaacc ctcccgccat ttttttaaaa
    14881 cctgtccgcg cgatacacaa ttaatcgttt tcaggggctt tttagaagcg tctaggattg
    14941 atttttatat cttgggcagg ggtttgtatt atccgcataa aaaaacgcgc taaaaagcgc
    15001 gtttttgagg gttttataaa ttcacggtat cagacccgaa aaataatcct gcacatcgtc
    15061 catcaaagcc tgtttgtcgt cgtccgtcag cgtcaaaaat tcccgttgcg gaatccgaac
    15121 tttgcggttg cgccccgcca taccgccgaa gttgtggatg gcggcataga cgatattcgt
    15181 accgacaagg gctgtatcgt tgtctgacag tgtggaaaaa ctgtctttca gacgacccga
    15241 atccgaaagc ggcttgccgt cgcggtattt tagccccaac catttcggac ggcctgcgta
    15301 gcggaaattg agcttgaccg ccgtgtgcat ggtttcggac agtcggcgca tcagcaggta
    15361 gcggttttcg atcccgttgc caagccgctc gatttggttt aggacgacaa agatattgtc
    15421 tattttgaca tcaaccatgg tattatcctg cctaggctac taaaaaagcg gtgaatttgt
    15481 ctgccctccg cagcccttat gcagtcctcg gactgggatt ggtatccgtt acataagcga
    15541 gagtgtgtcg ggattgcagt ccgacttagt agcctttttt catttcatag cgtttcttac
    15601 ttttcacaat ttctttttca tccgccctgc gaaacgaatc caaaaaaatc gaatcgtact
    15661 gtggcagata tttcaaaact gccatcaatc tgtatttttc gttcccctcc aacggttttc
    15721 cgagcaaaac aaaactttct tttcctctgc ttttgtcata aaaaatatat tcgggctcat
    15781 gaatcagatc ggggagcatg gcatataaat ctgttccaaa cgattgcccc tcccgactgt
    15841 ccacctgttt gaccaatgta tcatcagaca gccacaccgt cgctcgagtc atacctgcca
    15901 gttcttgcat ttccttgctc aatacacccg cagcaaattt aagctgtctt gatagcgcgt
    15961 ttcggatttt gattttctgc tctttatcgg gcttgccatc aatattcaaa cgttgcttga
    16021 cttcataaaa ctctttttca agctgtttaa agctggtttt aaaatccgca ccacccattt
    16081 ccgctttggc aaattgatgc gccaacgcac ggtcgtactt gtctaaatcg gggcggtagt
    16141 tcattcgtcc ggcgttgtaa tcaaatcctc gatcggtcgt gtacagactg ccatccggtg
    16201 ctttataggc aagggtcaga taagtatcgc ctttcttatt gtagatttta tgggtctcaa
    16261 caagattgtc cgacgtgctt tgcccgacaa tccgcccctg gcgttccaca tcccgctccg
    16321 acagcgcgat gaccgagcaa cggcagttgt agccgttggg cgggtaaaac gttgcccaaa
    16381 acgggtcgtc gtagcggtac accagcccgt ctattgccga atgcgccgga cgggtgcggc
    16441 tgtcgcctac cgcgtcatac atccaataag gtcgtgcatc aatatttgcc atatatcctt
    16501 gatattgacc ggcgttgtag gcagtttgca tgttggtacg gtaaatcgtc tccaacctcc
    16561 gcggcgaacc gaatacctcg ccggtggctg ggtcgatgat atccttaccg ttatgcccgt
    16621 tcggatgcag ccagcccttg ttgctgagca gattcaagat accttttcgc caatcgtcaa
    16681 acgactgtcc actttttgcc gattcaacca tcgaagtttt gatgtcgttg agcatatcca
    16741 agtcggtcat gcgggcaatc gtatagactt tggcaatttc ggaggctgtc agattgcggt
    16801 agctctccgc cgtaaccttt ttactttcca gccactcgat tgcctttttt ggcggcagac
    16861 ttaaggcaaa tcccaaatca ggcgcgggca tggtcttgtc ccaaaatatc gctgataaac
    16921 aaggcctgct gcatataggt acgcagtttc gcgttgtcca aattcggata aagcgcattc
    16981 agtgcggcat ctgcctcctc gtagctgttg caagcattaa gtgcggcaac cgcctgacgc
    17041 accatcgggt tgagctgaga attgaaatcg ggctcaacca gcgcgtcatc caacgcgccg
    17101 tccaatattt cctgatgcct gcccgtagcc ttagatggta cggtgtgggc ggataaagcc
    17161 gccaatgcag ttctgtttat cggattgtcc ggtacctgcc gcaccaacac agcctcaccc
    17221 tcctgcacat ctggaatgac cagtttgtcg cgcacccagc tttcggggat ttgtacgccg
    17281 acatccacca gtttcgggat agcgtcggca aagaccgcga tgtctttcgg ctcgcgcgtg
    17341 tcaaattcaa atttcggcac gcggtttggg tcggcattgg gatagttgat ttgcaggaac
    17401 ggtccgatga tttggcttgt gatggtttgc gccacctgtt ttgcgtccga caccagcaaa
    17461 tcgcggcgta cctcgttgtg gatattgccc agcgcgttgg tgctggattt tccgtccgca
    17521 ccgctggtta gcgtttgccc caaaatcagc cgcgccgccg atttttcgca ccagtcggcc
    17581 atctgcaaaa acggattgct ggttgccatc gtaccgcttg cagcgttgtg cagctcgatt
    17641 tccatacctt ctggcatgat gcctgccgcg ttgtgaccga tttccgccac cgctcgaagc
    17701 agggtgtttt tttcctcttt ggttgcgccc gcgccgtatt tgccgatacg gatgggcatg
    17761 ccgtacagct ccaaaaactc ggcaaaatcg tggacggcgt agtgtttgaa catatacagc
    17821 caggaaagcg tgcggaaaag cccgttgcgc gcctgctgga ggctgcgcga tttttgggta
    17881 tgaacgaccc agcccagcgg ccacaacgct tcgccttccg gattttcgcg ggtacgcagc
    17941 agcagcccgt tgtctttgtc ccatttgaac cagctttgcg ggcggtggat aaagtttcgg
    18001 ggtaggtaaa ggccgtctga aaatacccac tcgacctcca acgcagaaaa tccgtgccct
    18061 accgcgtcca tcaaatccat aatcaggtct tcgagggtag gcaggctgtc catcatttcg
    18121 taggcttggt cggacagctt ttcttcttcg ggcgtcgcat ttcgcggcgg ggcgacgcgc
    18181 cagttgagcg tcagcagcgc gcgtttgcgc gtccccatat ttgccgcgat gtcgctgtcg
    18241 cgctcctcaa tgtccgcgaa aagctcgtgt tgggcgcgga tgtcgccgct ttccgcatcc
    18301 tcgaagagag cccgcatctt ttgcggtgta ataaaattgg acggatgctc ggcgataacc
    18361 cgaccggtcg ccgtaatttg agccacgtcc gtctgcaatg cagcttcggg cttttggatt
    18421 ttagttttat tgttcttttt tgccattatc ggctccattt actttttatc tcgtcatcaa
    18481 aatcgttatc ggtagggctt tgccattcta tcggcacaca attggccacc gcccccgacc
    18541 agagcatatg cactgcatca ggaccatcat catgatcagc ctttggaaaa tggcggaact
    18601 gctgtatcag cgtctgttgg ctctcattca acagaatcaa accgttcgcc atgtgaggct
    18661 gtaaagtctc aatccgcaag agcttgtccg ataccggctt gaccgcccgc gccgggacag
    18721 gtattccacg cgccgcgctg cgcttgacca gctcatcttt caaaaattcc tgaaactgaa
    18781 ccgtctcaac aaaccacagt ttgcaacgat attgacggtg caatctgata acgtcctcaa
    18841 taatcaaatc agggagacgt tttttaatct gagcttccac gacatacagt ttgccggttt
    18901 cacgttgata accgccaatg ataatcgccg acgggtcacg gctcgccccc gctttaccca
    18961 gcgacgggtc gagcgcaccg aaatacacca aatcggacgg caggtcgttc caaaacttca
    19021 tcgacttggc aaacggcgca tcttcgccgc tgaccgggtc gttttgatat tcgctgtcaa
    19081 atgtcgcatg accgtcacgc gcacggattt tcatcagcgc gagtacgccg cgagccgccc
    19141 agcttgtttg cgcaccgcgc tccatctcgt ctttattggc gagataaaac gcctcggcca
    19201 cagtctcgcc gtcgtttcgg aaaagttcct cccatctgtc ccacaaatcc atgcggtcgg
    19261 gccagcgttt catcgcctta aacttaatac cgtgccaaaa cgggttattc aaagtgcggt
    19321 taagtacgct gccgtaatgc aaaatcgtgc cgatatatat cacatcgtat ttctggccga
    19381 caccgcccaa aggcaataca gtcttagtca gccacgcatt gagcttgtcg cgctgttcgg
    19441 ggttgcggac ttgctcgtca ttctcaatat cgtccaaaac agttaagtca ggacggtaag
    19501 ggccgtgacg caaaccgcgc agctttttac cgctaccggc cacttggact ttaacgtcat
    19561 tggccgtcac aatcgtaccg gcctgccata cgcggccctg cccgcatact tccggaaagt
    19621 cggttttcaa gcgcggatta aattccaatt ccgccttgat ggcttcgagc atcgggtatg
    19681 cctgatctat gctgtccatc acaataacgg cataatgttt ttggcctgtc acaatacacc
    19741 acagcgtaaa caactgagta acctgcgtcg acttaccctc gccacgcggc gcacccaccg
    19801 cctcattttc ccctttgggg gagcggataa tctccggcag acggctgaat aaaaacgcat
    19861 gcagttcgga tttttcaggg gagcggatat aatgcggaaa gtaggtgttg acgaaatact
    19921 cgtaaccgcc taccgggtca aacaccttgg cacggcgtgc agcaatagcc ttgggcgacg
    19981 catcgaagcc gtccacctct gcctcaataa tttggcggag ttgggcggca tattcggcaa
    20041 gtgactttaa aaactctttg gacttcattc aattacctta tgcagttgct acctgtattt
    20101 cttttcaatt tccacaccca acggctcgac cagctcgaca taagcctgca agtgttgcgg
    20161 gtatcgctcc ctgaccactt cgccgaacaa ttccaacacc tcgattgccg tcgccagttt
    20221 tgacgtttcc ggcatcactt tggcgtttgc cgccacggtc ttggtaaacg cgtcggacag
    20281 gcttgtcaac agtttggcgc gctcggacgg catcagccct tcaatcgacg tgtcttgcaa
    20341 cattgtcatc gttgactggt actgcaccaa aaaacccgcc agcagcgaac ggctcaagtc
    20401 ttcgatgccg ccgcctgcca aggtataggc ggcgcgcact ttgtcccaat cgtcgctgct
    20461 ttccttggcg gcgcgtttcc agctgcgggc ggtcgcaatc gggatttcgc acatcatcgc
    20521 cgcgatttca agcgtctgcc cgtcgctgac gtacaatcgg cgcagctttt cgcggatttc
    20581 cttcggatgc gccatatcag cccccgaact tggctctcag cagctcccag ccggtcgtta
    20641 caatcacacc gccgagaccg ccgtaaaccg ccgcagattt cttgcagtct ttcttaattt
    20701 gctgcaattc ctcgtccatt cgagcctgat tggcgagcat gtcatcctgc ttggcttcga
    20761 tacgcgccaa gacttccaaa atcgggtcgc tcatgatttg tccgctttcc tgtccaattt
    20821 ttcgtttact ttttccaact tgttttcaat tcgttctaag gctgccgcaa tattagtgct
    20881 gtctgccttg gcatcttgct tggtgtgata agagagcttg accgcgtgca gctcctcttt
    20941 aaggtcgtca atgcgcttgt ccgcctcttt cagacggcct gaaatgccgt tgacccaaaa
    21001 ccaaaacgcc gccgttgcaa tcggccacag ggttctgaaa ccaaattcaa agtccattta
    21061 aaaccccttt aaaccggcac atcgccaaat acgatacgga ccgcgtagcc ttcgggatga
    21121 cgactcgccg cctcgacctt ttggccgtct gaaaagactg agtaatattt ccgcaaaata
    21181 ccaatcacat cagcaggggc gtcgcggaaa actccacaca aaaggtcgtc tgaaaatcct
    21241 tatccatgcg taccgcgtaa tcgataccgg ccttatccaa caaatcggaa acatgaatga
    21301 caaacggctc ttgctcgcgt gcgcggctca atcccagctc taaatccgca tggcggcagg
    21361 cgaccgtgcg ttgcaccaac tcacgataag tcgtcatcgc gcaccctccg aaccgtcaac
    21421 tgccgcttga ctgttg
//
LOCUS       DAWKOG010000019        20880 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.019, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000019 DAWKOG010000000
VERSION     DAWKOG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20880
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.019"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(97..1116)
                     /gene="lepB"
                     /locus_tag="WJK66_000537"
     CDS             complement(97..1116)
                     /gene="lepB"
                     /locus_tag="WJK66_000537"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEZ7578291.1"
                     /translation="MNIMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNVFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRNKVLTVNGKPASDIPDGTYRYPDDTDP
                     SEIHNTDMFHSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            complement(1259..3052)
                     /gene="lepA"
                     /locus_tag="WJK66_000538"
     CDS             complement(1259..3052)
                     /gene="lepA"
                     /locus_tag="WJK66_000538"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEZ7578292.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPAGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     IANPASEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKNGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            complement(3196..3897)
                     /locus_tag="WJK66_000539"
     CDS             complement(3196..3897)
                     /locus_tag="WJK66_000539"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEZ7578293.1"
                     /translation="MSLKTVAVIGAMEQEIKLLREMMENIKAVSFGRFSAYEGELAGK
                     RMVLALSGIGKVNAAVATAWIIREFAADCVINTGSAGGLGKGLKVGDVVIGTETAHHD
                     VDVTAFGYVWGQVPQLPAVFVSDGLLVGKAKQAAEVFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPKVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AASSAKMVAEIVKSL"
     gene            4083..5195
                     /locus_tag="WJK66_000540"
     CDS             4083..5195
                     /locus_tag="WJK66_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ7578294.1"
                     /translation="MTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDE
                     PLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLLDTSRFRVNAMIQRGATALVFRA
                     ITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNENSFGHII
                     TIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYA
                     IAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRD
                     GGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGDI
                     SLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            5229..6206
                     /locus_tag="WJK66_000541"
     CDS             5229..6206
                     /locus_tag="WJK66_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEZ7578295.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TVSGFRLRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            6210..6560
                     /locus_tag="WJK66_000542"
     CDS             6210..6560
                     /locus_tag="WJK66_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HEZ7578296.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            6565..7344
                     /locus_tag="WJK66_000543"
     CDS             6565..7344
                     /locus_tag="WJK66_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ7578297.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     SEVFAIAEAHEHIYCTIGVHPDSKEAEEFSIAEMVAAAASPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRTV"
     gene            7370..7585
                     /locus_tag="WJK66_000544"
     CDS             7370..7585
                     /locus_tag="WJK66_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HEZ7578298.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREVKKD
                     ESGGGYALCHLPDSRIVEEWEYFRSQH"
     gene            7686..7997
                     /gene="grxD"
                     /locus_tag="WJK66_000545"
     CDS             7686..7997
                     /gene="grxD"
                     /locus_tag="WJK66_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEZ7578299.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            8103..8729
                     /gene="upp"
                     /locus_tag="WJK66_000546"
     CDS             8103..8729
                     /gene="upp"
                     /locus_tag="WJK66_000546"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEZ7578300.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLAIELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPVSYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            8752..9072
                     /locus_tag="WJK66_000547"
     CDS             8752..9072
                     /locus_tag="WJK66_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HEZ7578301.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLDKEAAEQGLIWFAEHVADARAHPGKHPNIDLLEKVVQSGETLLLKP
                     LAAQ"
     gene            9323..9745
                     /locus_tag="WJK66_000548"
     CDS             9323..9745
                     /locus_tag="WJK66_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578302.1"
                     /translation="MKKMFLSAVLLLSAAAQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRRATMKFQNGEFMYTVWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPQTAQANFNDADFSR"
     gene            9763..10524
                     /locus_tag="WJK66_000549"
     CDS             9763..10524
                     /locus_tag="WJK66_000549"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEZ7578303.1"
                     /translation="METVKPIMLIVRPSGRAKDDVETCRRAGWQAEVLSPIEIEADAA
                     GLELLSEQYARADAVFWVSPTAVETAVPYLNLSDGIKMHISVGQGSRRVLERCLGRTV
                     IAPEDGNDSEAVLRLTVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEVAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            10539..11873
                     /locus_tag="WJK66_000550"
     CDS             10539..11873
                     /locus_tag="WJK66_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ7578304.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAVCALVLAALGLGTSGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQAAIQSELDRLDGNVKANGEQILEMQKSYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFDQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLILDGVLKPGVQVKNEAASASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNSEVYQGDLNNAEAAVRQYFDAKS
                     PATQSWLKELAELKALDVRMTADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NENTASEPAAASDVKTIEAPSLPSERKPEQPAKKQPVPEKAGRSPSAKGERA"
     gene            11870..13087
                     /locus_tag="WJK66_000551"
     CDS             11870..13087
                     /locus_tag="WJK66_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HEZ7578305.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVFNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLVNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDETGEPQKAEAQRNLVLA
                     SVAEENRPSAETH"
     gene            13162..14226
                     /locus_tag="WJK66_000552"
     CDS             13162..14226
                     /locus_tag="WJK66_000552"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEZ7578306.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTELATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHEDDIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGSS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAEIGADALGLDWTCNIGE
                     ARRRVGKQVALQGNFDPFALFGTPESIRTEVARILADYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            14415..15794
                     /gene="radA"
                     /locus_tag="WJK66_000553"
     CDS             14415..15794
                     /gene="radA"
                     /locus_tag="WJK66_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEZ7578307.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSAVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTDGVNLLAEIRMEAIQAA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICRDSRE"
     gene            complement(16070..16549)
                     /locus_tag="WJK66_000554"
     CDS             complement(16070..16549)
                     /locus_tag="WJK66_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEZ7578308.1"
                     /translation="MKHILPLIAASALCISTASAHPASEPSTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTVGE"
     gene            complement(16791..17150)
                     /locus_tag="WJK66_000555"
     CDS             complement(16791..17150)
                     /locus_tag="WJK66_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEZ7578309.1"
                     /translation="MNIKHLITAALIASAAFAAQAAPQKPVSAAQTAQHPAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            complement(17189..20803)
                     /gene="recB"
                     /locus_tag="WJK66_000556"
     CDS             complement(17189..20803)
                     /gene="recB"
                     /locus_tag="WJK66_000556"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HEZ7578310.1"
                     /translation="MSASIQAFDPLTVPISGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRGRLDDVLQVLESKGIAKLGDDTLSDGIAAYCAE
                     HHEGDTFLPELLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVEMTEEDGDRLLIPAQDFWRERVSNDPVLAALALKRKAVPQTVLAQISRYLSR
                     PYLNFRRPQADLKQAQRNAETSWQTICRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHDRLLKLASDKLEAGLKKGKTPDAAVFAELQKLADFGRD
                     LNALEEAEEATMIRLQLDLIEYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHADT
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPERPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAARGRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSSIFGYDAQQLHDFNQNESEILHWAESARTALDNWNKYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLGDQISLAENNSGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQGANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSTNTADNPLAYLIEGSPQ
                     DSRETVRRAYACEKDGIAMLKRNWRRVADNAPSGTNFAFTENAPPPAAYRSNAGQTAE
                     FAANSIPERGFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAGQETLIADKLKKYGFEEIWLPAVAEM
                     AEACRKTPLTGAYDLSDISPECRCPEMGFTLHTEDFSLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFVDMVCQDPDGNICVIDYKSNHLGTDASAYTQQAMDEAVAHH
                     HYYLQALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK
                     "
BASE COUNT         4785 a         5444 c         5861 g         4790 t
ORIGIN      
        1 gtaggaaaat ccgacaacgt tagatccgcc tgcaaaagtg aggaatagca aatgccgtct
       61 gaaactattt tcagacggca ttcttggctt cctggcctaa cggattgccg taccggccct
      121 gccgaaatcg ccgaagttca tcaaaatgaa cattgccttg ccgacaacca gcttgtcatc
      181 cacaaatccc cagtagcgcg aatcggcact gttgtcgcgg ttgtcgccca tagcgaaata
      241 gcgtccttcg ggaactttgc acacgaaacc gctgccgtcg tcggcatatt ggcagtgttc
      301 caaaccgctt tgctctatgg aatatccgtt ttcagacata atatcggagg tatatttgcc
      361 caatacgggc agggaaacgg caggctgtcc ttcttttttc agaatattga aggatttgcc
      421 gtccaaaccg ctgtggaaca tatccgtatt gtggatttcg gatgggtcgg tgtcgtcggg
      481 ataacggtat gtgccgtcag gaatatcgga agcaggtttt ccgtttaccg tcaaaacttt
      541 attccgatat tcgaccacat cgcccggaat gccgacaata cgcttgatgt aggtcatctc
      601 cggttgcaaa ggataattaa aaacgaccac atcgccccgt tcgattttgc ccgtggggat
      661 gaatacattg tttaaaacgg gtacgcgcag gccgtaggaa aatttgccga ccaaaatgaa
      721 atcgccctta atcaggcccg ggcgcatcga gctggacggg atttggaacg gttcggcaat
      781 aaacgaccgg atgaggaaca ataccaaaac ggtagggaag aaactgccga aataatcgcc
      841 gaagtggctg ctttccgaga tttcgggatg agtcttcagg cggtatttgt atacccccca
      901 agccgtaccg cacaatacaa cgaaaatcag gaaaacggcg gtaaagctca taaacaggga
      961 caaagcggca aacacgccga ccgccgtcag gatataggcg tattccagac cggaactcca
     1021 ttcaccgttt tcctgccgct ttttgtcgct tttgaaataa aggatgatgc cggcaagcag
     1081 cgcggcagcc gcgcccgaca ttagcataat gttcattgtt gttccttaac ggttaaaaac
     1141 ccgcccgtcc gtgcaaccgt ttttaagagg cggtaaatca caaagtttgt tggcggacgt
     1201 gctctcttac aatcagggcg gtttaagggg catgatgcac tgccccgtgt gccggatatt
     1261 atttgtcgct cacctgcaaa attgccaaga acgcgctttg cgggatttcc acattgccca
     1321 cttgtttcat acggcgtttg cctgcttttt gtttttcaag cagttttttc ttacgcgtaa
     1381 tatcgccgcc gtaacatttt gccaaaacgt ttttacgcag ggctttgacg ttctcacggg
     1441 cgataatctg actgccgatg gcggcttgga cggcaatatc aaacatttgg cgcggaatca
     1501 gctcgcgcat tttcgatgcc agttcgcggc cccggtgaac cgcgctttga cggtggacaa
     1561 tcaggctcag cgcatcgact ttttcaccgt tgaccatgat atccagctta atcaaatcag
     1621 acggttggaa ctctttgaaa tggtagtcca acgaagcata gccgcgcgaa gtagatttga
     1681 gtttgtcgaa aaagtccatc accacttcgt tcatcggcaa gtcgtaagtc agcatcactt
     1741 ggcggcccat atattgcata ttgacttgca cgccgcgctt ttggttgcac aaagtcatga
     1801 cgttgccgac atattcctgc ggcacgagga tggttgcagt gataatcggc tcgagaatgg
     1861 tttcgatgct gccgatgtcg ggcagtttgg acggattttc gacttcgatt ttctcgccgt
     1921 ttttcaacac gacttcataa accaccgtcg gcgcggtggt aatcaagtcc atgtcgaact
     1981 cgcgttccaa acgttcctgc acgatttcca aatgcaacag gcccaagaag ccgcaacgga
     2041 agccgaaacc caatgcttgg gaaacttcag gctcgaattt caacgaagca tcgttaagct
     2101 gcaatttttc caaagcgtcg cgcaaggctt cgtagtcgtg gctttccacc ggataaagtc
     2161 cggcgaatac ctggctttgc acttcttgga agccgggcag cggctcggag gcggggttgg
     2221 caatcaaagt aactgtatcg ccgacttttg cctgtcccaa ttcttttacg ccggtaatca
     2281 aaaagcccac ttcgccggct ttgagttctt gtttttgaac tgatttcggc gtgaatacgc
     2341 ccagctgctc aacctgcgtt tccgccttgg tgctcataaa gcgcactttg tctttcagct
     2401 tgatggtgcc gtttttcaca cgaatcagca taaccacgcc gacatagttg tcaaaccacg
     2461 aatcgacaat caccgcttgc agcggcgcat tttcatcgcc ggccggtgcg gggattttgg
     2521 caacgatttc ttccaaaacg tcttccacgc cgatgccgct tttggcggaa cattgcaccg
     2581 cgccgacggc atcgatgccg atgatatctt cgatttcctg ttccacgcgt tcggggtcgg
     2641 cggcgggcag gtcgattttg ttcaaaacgg gcacgacttc aacgcccaaa tcaatcgcgg
     2701 tgtagcagtt cgccacggtt tgcgcttcca cgccttgaga tgcgtcaacc accaaaagcg
     2761 cgccttcgca ggccgacagc gaacgggaaa cttcgtaaga gaagtcgacg tgtcccggcg
     2821 tgtcaatcag gttgagctga tacacctgcc cgtcgcgcgc tttgtagttg agcgcggcgg
     2881 tttgcgcttt gatggtgatg ccgcgctctt tttcgatgtc catagaatcg agtacctgcg
     2941 tactcatttc gcgcaaatcc aagccgccgc agtattggat aaaacggtcg gcaagcgtcg
     3001 atttgccgtg gtcgatgtgg gcaatgatgg agaaatttcg gatatttttc attagagtta
     3061 ttttgaatgt cggacagtgg gttttggaaa tgccgtctga acaaacggcg ctgcgtccga
     3121 atgtcgggcg caacgggata aacagcccga cattctagcg gaaaaccgct gttttggcat
     3181 gatttgataa aggttttata aagatttgac gatttctgcc accatttttg cagaacttgc
     3241 cgccgccgtt ttcaaaaatt cgtcaaagct gatgtctgcc ttttcgtccg ccgaatcgga
     3301 aaccgcgcgg atgatgacga aaggcgtttc caactgatga caggtttggg caatcgccgc
     3361 cgcttccatt tccactgctt tgactttggg gaagtgcttg cggatttccg ccacgccttc
     3421 gctgctgtgg acgaagcggt cgccgctgac aatcaggcct tgttctaccg ccgcgccttc
     3481 aaacacttcc gccgcctgtt ttgctttgcc gaccaagagg ccgtctgaaa caaatacggc
     3541 gggcagttgc ggcacttgtc cccaaacata gccgaatgcg gttacgtcga catcgtggtg
     3601 tgcggtttcc gtgccgatga ctacgtcgcc gactttcaaa cccttgccca aaccgcccgc
     3661 gctgccggtg ttgatgacgc agtccgctgc gaattcacgg ataatccaag ccgttgcaac
     3721 cgccgcgttg accttgccga tgccgctcaa tgcaagcacc atgcgttttc ccgccaattc
     3781 gccttcatag gcggaaaatc tgccgaaaga gacggctttg atattttcca tcatctcgcg
     3841 caaaagcttg atttcctgtt ccattgcgcc gataacggct actgttttca aagacatatt
     3901 gctgacctgc tgtgaatttc ggatagaatg ccgaattata cacgctaaca cggcaggatt
     3961 gagtggaggt ggtttgtccg tgccgtctga aacggtttca gacggcacgg cgggtttttg
     4021 gtagaatggg aaggtacaga ttgtttgaag attaggggac gaggatgttt accgatgaaa
     4081 atatgaccgc aaaggaagaa ctgttcgcat ggctgcgcca tatgaacaaa aacaaaggtt
     4141 ccgacctgtt tgtgacgacc catttcccgc ccgcgatgaa gctggacggc aaaatcaccc
     4201 gcatcacgga cgaaccgctg acggcggaaa aatgtatgga aatcgccttt tcgattatga
     4261 gtgcgaagca ggcggaagaa ttttcatcga ccaacgagtg caacttcgcc atcagcctgc
     4321 tggacaccag ccgcttccgc gtcaatgcga tgatacagcg cggcgcgacg gcgttggtat
     4381 tccgtgcgat taccagcaag attcccaagt ttgaaagcct gaacctgccg ccggtcttga
     4441 aggatgtcgc gctgaaaaaa cgcgggctgg ttatttttgt cggcggcacc ggctcgggca
     4501 aatcgacttc gcttgcctcg cttatcgatt accgcaatga aaattcgttc ggacacatca
     4561 tcaccatcga agacccgatc gagtttgtcc acgaacacaa aaactgcatc atcacccagc
     4621 gcgaggtcgg cgtggatacg gaaaactgga tggcggcgtt gaaaaacacg ctgcgtcagg
     4681 cgccggatgt catccttatc ggcgaaatcc gcgaccgcga aacaatggac tacgccattg
     4741 cctttgccga aacggggcat ttgtgtatgg cgacgctgca cgccaacagc accaatcagg
     4801 cactcgaccg catcatcaac tttttccccg aggagcggcg cgaacaattg ctgacggatt
     4861 tgtcgctcaa ccttcaggcg tttatttcgc aacgcctcgt tccgcgagac ggcggcaagg
     4921 gcagggtggc ggcagtcgag gtgctgctca attcgcccct gatttcggag ttgattcaca
     4981 acggcaacat ccatgaaatc aaagaagtga tgaaaaaatc cactaccctg ggtatgcaga
     5041 ccttcgatca acacctttac caattgtatg aaaaaggcga tatttccctg caagaagcat
     5101 tgaaaaatgc cgattccgca cacgatttgc gtttggcggt acagttgcgc agccgccgcg
     5161 cgcaaagttc cagccccgat ttggaactgc tctgatggcg gtatggattt ccggacggat
     5221 ggtttgaaat gatttatccg tggcataatg agcaatggcg gcagattgcg gaacattggg
     5281 agcgtcgtcc caatgcatgg ctgtttgccg gcaaaaaaga tacggggaaa actacatttg
     5341 cccgctttgc ggcgaaggca ctgttgtgcg aaacccctgc accgggctgc aaaccctgtg
     5401 gcgaatgtat gtcctgccat ctgtttggac agggaagcca tcccgatttt tacgaaatca
     5461 cccccttgtc ggacgaaccc gaaaacggac gcaaactgtt gcagatcaaa atcgatgccg
     5521 tcagggaaat catcgataat gtgtacctga cttcggtacg gggcggtttg cgcgtgattc
     5581 tgattcatcc tgcggaaagt atgaatgtcc aagccgccaa cagtttgttg aaagtgttgg
     5641 aagagccgcc gccacaagtg gtctttttgc tggtcagcca cgcggcggac aaggttttac
     5701 cgaccattaa aagccgctgc cggaagatgg ttttgcccgc gccttcccat gaagaggcat
     5761 tggcatatct gcgtgaaagg ggtgtggcgg aacctgagga acgtctggct ttccattccg
     5821 gagcgccgct gtttgatgag gcggacggtg tccgtgcgtt gcggattaaa ctgttggata
     5881 ttttggcaga accaaggttg ttgaagattt tggattacgc cgcgcttttc gataaggaaa
     5941 aacttccgct cgccgtattt gtcgggtgga tgcagaaatg gctggtcgat ttgggattgt
     6001 gcctgcaaca catgaaaccc gtctattatc ccgcttatga agacaggctg cttcagacgg
     6061 tatccggttt ccgcctgcgc aatgtatttg cggcggagga tatgctcaaa cagcttgccc
     6121 cctacgggtt tcatacttta aatgtcaaaa tgcagatcga gcatctgctc atcaactatt
     6181 tggaattgaa gaaagagaac gggtgaatta tgtcagacgg acaaaatatt ccggcaaaaa
     6241 tgatgtcgtt gcagctgaaa gacatgaatc tgctgtacag ctcctacatg ccgtttttgg
     6301 aacacggcgg tctgtttgtg cagaccaacg acgtattttc catcggggac gatattctgc
     6361 ttgccgtaga aatcctcaac ttccccaaac tgttcctgcc gaccaaagtc gcctggatca
     6421 atcctgcgcg tacttcctcc aaacccaaag gggtggggct ggcattcaca aaacacgaaa
     6481 actgcctgaa agtcaaagac cagatcgaag tcgaactggg caacacaatc ggcggcagca
     6541 gacctacgtt taccatgtaa cgccatgcat atcatcgatt cgcactgcca cctcaatttt
     6601 gaaggtttga aagaacgcct gcccgaagtt ttgtccaaca tggaagcaaa cggcgtgggg
     6661 caggcgctcg ccatcagcgt cagtagggaa agcttctccg aagtctttgc catcgccgaa
     6721 gcgcacgaac acatctattg caccataggc gtacatcccg acagcaagga agccgaagaa
     6781 ttttctattg cggaaatggt tgccgcagcc gcctcgccga aagtggtcgg cattggcgag
     6841 acgggtttgg attattactg gtgcaaaggc gatttgtcct ggcaacacaa acgctttgca
     6901 gaccacatcg aagcagccaa tcaaaccgga ctgcccgtta tcgtccatac gcgtgatgcg
     6961 gcggcggaca ccttgtctat cctgaaagaa tgccgggtta attcgggcgt tatccactgt
     7021 ttttccgaag acatcggttt tgcccgtgca gcaatggatt tggggcttta tatttctttc
     7081 tcgggaatcg ttacctttaa aaacgcaccc ttggttcagg aggcggcaaa atacgttccg
     7141 gacgaccgca ttttggtgga aaccgatgcg ccgttcctcg cccccgttcc caaacgcggc
     7201 aggcagaacg aaccggcttt tgtgcgccat accgccgaac atatcgccaa attgcggaac
     7261 caaacattgg aacaggttgc ggcatatacg acggaaaact tttaccggct gtttaaaaaa
     7321 gtacccgata tgcggaccgt ctgaccctgt accgacgata aggaaaacca tgaaggcaat
     7381 tcatccgtat gcatgtccgc gctgctgccg gctgcctgcc aacacgtttc ggacaggcat
     7441 ggcaaattcc gcttccaaat tctgcattgc caaaggcggc agacgggaag taaaaaaaga
     7501 cgaaagcggc ggcggatatg ccctgtgcca tttgccggac agcaggattg tcgaggagtg
     7561 ggaatatttc cgttcacaac attgatactg cgcgatatac ggcaaatatt gtgggaagtt
     7621 tccgcttttg cgtataatgc gtcctacctg acaaattttg tcaactttat caaaaggaat
     7681 aagcgatggc ttccatccac gaccaaatta aagaagtagt aacgacacac cgcgtcgtat
     7741 tgtttatgaa aggtacgaag cagtttccgc aatgcggttt ctcttcccgc gccgtgcaaa
     7801 tcctgaacgc ggcaggctgc accgattacg ttaccgtcaa cgtattggaa aatcccgaag
     7861 tacgccaagg cattaaggaa tacagcgact ggccgaccat cccccaactt tatgtgaacg
     7921 gcgagtttgt cggcggttcg gacatcctga tggaaatgta tgaggcaggc gagctgcaag
     7981 agctgctgaa agcctgatgg attcggcaat gccgtctgaa cgtgtttcag acggcatttt
     8041 cttttccggc aaatcaaaaa aaagtataat ggcgcgtctc aaaatcacac tggaacaccg
     8101 cgatgaacgt taatgttatc aaccatccgc tcgtccgcca caaattaacc ctgatgaggg
     8161 aggcggattg cagcacctac aaattccgga cgcttgccat cgagctggcg cgcctgatgg
     8221 catacgaggc aagccgtgat tttgaaatcg aaaaatacct tatcgacgga tggtgcggtc
     8281 agattgaagg cgaccgcatc aagggcaaaa cattgaccgt cgttcccatc ctgcgtgcag
     8341 gtttgggtat gcttgacggc gtactcgacc tgattccgac tgccaaaatc agcgtagtcg
     8401 gactgcagcg cgacgaggaa acgctgaaac ccgtttccta cttcgagaaa ttcgtggaca
     8461 gcatggacga acgtcctgcc ctgattatcg acccgatgct ggcgacgggc ggttcgatgg
     8521 ttgccaccat cgaccttttg aaagccaagg gctgcaaaaa catcaaggca ctggtgctgg
     8581 ttgccgcgcc cgagggtgtg aaggcggtca acgacgcgca tcccgacgtt acgatttaca
     8641 ccgccgcgct cgacagccac ttgaacgaga acggctacat catccccggc ttgggcgatg
     8701 cgggcgacaa gattttcggc acgcgctaac tgactgattt tcggagttga tatgaatttt
     8761 caagactatc tcgccacatt tccttcaatc gaccatctgg gcggtttgga cgttcaggat
     8821 gcagatggca aaacggttca ccacattcct gccgttcagg gtaagctcgg ttcgctcaag
     8881 ttgtacaacg ctttggcaga acgttttgac ggaaaattgg acaaagaagc ggcagaacag
     8941 ggtttgatat ggtttgccga acacgttgcc gacgcgcgcg cccatcccgg caagcatccc
     9001 aatatcgacc tgcttgaaaa agttgtgcaa agcggcgaaa ccttgctgct caagccgctt
     9061 gccgcgcaat aattttcgac catgccgtct gaaatccgtt tcagacggca ttttgtcgga
     9121 aagaagaccg taaaacgggc attttctttt ctatttcagg atacgggcaa tgatgtttca
     9181 acacacagga cgacacataa agcgttgccc tatgtgttgc cctaatttgg aaggggttac
     9241 acccctctca aataacatct gatgctgctg ccacgaagga cggatgtcca aatggcggag
     9301 tttcaaccat taaggaaata cgatgaaaaa aatgttcctt tctgctgtat tgcttctgtc
     9361 ggctgccgcc caaacggtgt gggcggatac ggtgttttcc tgtaaaacgg acaacaacaa
     9421 atatatagaa gttcaaaaaa tcaaccgcaa tctttacgaa tattcgttcg gcagtgcggc
     9481 aaaaaaagaa attgccatac gcaacagcaa agctgacctg ttggggcgtt ccgacaggtg
     9541 gcaaggcatg ggtagcggac gccgggcaac gatgaaattc caaaacggcg aatttatgta
     9601 caccgtatgg acgggcttcg attccgtgac tcatacggaa agcagcggtg ttgttgtgga
     9661 gcgtaggggc aaggaagtcg cacgggttgg ctgtacgccg caaaccgcgc aggcgaattt
     9721 caacgatgct gatttttccc ggtaatcggg gcggataagg cgatggaaac agtgaaaccc
     9781 atcatgctga ttgtccgacc gtcgggcagg gcgaaggacg atgtggaaac ctgccgtcgt
     9841 gccggatggc aggcggaagt gttgagcccg attgaaatcg aagcagatgc tgccggactg
     9901 gaacttttgt ccgaacaata tgcccgtgcg gatgccgtgt tttgggtcag tccgaccgcc
     9961 gttgaaaccg ccgtcccgta ccttaacctt tcagacggca taaagatgca catttccgta
    10021 gggcagggca gccgccgcgt attggaacgc tgtttgggca gaacggtcat cgcgcctgaa
    10081 gacggcaacg acagcgaggc ggttttgcgc ctgacggttt ggaacagtct gcccgaaggt
    10141 gcgcgcgtat tgtttgtgcg cggacacggc gggcgggatt ttctgatgaa tgccttgcag
    10201 gagaaaggtt ttcggacgga ggtggcagaa gtctatttca gacggcataa acctttgaac
    10261 tttcaaaatt tccaaaccga aaatattgcc gccgcctata ttacgtccac agaactcgtg
    10321 cgcttgctgt tcggacagct tccgccgcaa ttttcccgat tcttcaaatc cttgctatac
    10381 tttacccatc atccgcgcat tgcggaggca ttgaagcgcg aaggcgtgtg ttcggtcgaa
    10441 accgtcccta cgctggaagc cgcgctttcc cattcttcca tttccgtttc agacggcatg
    10501 gtctttcccg gaacctcaaa ttaataagga gcaaaacggt gggcgaacct gaaaacaaat
    10561 catccgaacc cgtacgcgag atacaggcat caaaagaaat gccgtctgaa acctcttccc
    10621 cacgcaaaga aaacgaaaca gaagtacaca ttcctgccgc tccttttatc gtcaaacagt
    10681 ccggcagcaa cgctttggca gtctgcgccc tggtattggc ggcattgggt ttgggtacaa
    10741 gtggtttttt gtttgtccaa ggacagaatg tcttgaaaaa ccaagagctg gcattcaacc
    10801 aaaaaatcga caaagccgcc ttgggcgagt cggaaaacgc cgccctgttg aaagacaacc
    10861 tcaaccggca agccgccata caatcagagc tcgaccgttt ggacggaaac gtcaaagcaa
    10921 acggcgaaca aatcttggaa atgcaaaaat cctatcgcga gttgaccaaa ggacgcgccg
    10981 attggctggt ggacgaaacc gagaccatac tcaatctggc ggcgcaacag ctggtgttga
    11041 ccggcaatat ccaaacggca gtcggcgtat tggagcatat cgacagccgc ctgtcccgtt
    11101 tcgatcaggc agagcttctg ccgatcaaac aggcagtcag cagcgacttg gcggaactga
    11161 aaaaccgtcc ctatgtcgat atttccggca cggcattgcg cctcgacagg ctggaaaccg
    11221 ccgtatccgg actgccgctg attttggacg gcgtattgaa gccgggcgta caggtgaaga
    11281 acgaagccgc ttctgcttca tggtggcaga acgtatggga aaaatccctc ggcacattga
    11341 aggggctggt cgaaatccgc cgtttggaaa acaacgatgc catgctgatt tctcccgaac
    11401 aggcatattt tgtgcgtgaa aacctgcgcc tccgcctttt ggatgcgcgg actgcattaa
    11461 tgcagcgcaa cagcgaagtc tatcagggcg atttgaacaa tgccgaagcc gccgtcagac
    11521 agtatttcga tgccaagtct cccgccacgc agtcgtggct gaaagaactg gcggaattga
    11581 aggcgttgga tgtgcgcatg acggcggatg acggtttgaa aaacagccta aatgccgtcc
    11641 gcgcctatcg cgacggtacg cgcatgacgg cggcggaaaa tcaagaagca gaacaggcgg
    11701 cttccgaacc ggcaaacgaa aacacagctt ccgaaccggc tgccgcatcg gatgtgaaga
    11761 ccatagaagc accgtccctg ccttcggaac gcaaaccgga acagcctgca aaaaaacagc
    11821 ccgtaccgga aaaggcaggg cgttcgccgt ccgctaaagg agaacgcgca tgaaaacggt
    11881 agtctggatt gtcgtcctgt ttgccgccgc cgtcgggctg gcattggctt cgggcattta
    11941 caccggcgac gtgtatatcg tactcggaca gaccatgctc agaatcaacc tgcacgcctt
    12001 tgtgttaggt tcgctgattg ccgtcgtggt gtggtatttc ctgtttaaat tcattatcgg
    12061 cgtattcaat atccccgaaa agatgcagcg tttcggttcg gcgcgtaaag gccgcaaggc
    12121 cgcgcttgcc ttgaacaagg cgggtttggc gtattttgaa gggcgttttg aaaaggcgga
    12181 actagaagcc tcacgcgtgt tggtcaacaa agaggccgga gacaaccgga ctttggcatt
    12241 gatgttgggc gcacatgccg ccgggcagat ggaaaacatc gagctgcgcg accgttatct
    12301 tgcggaaatc gccaaactgc cggaaaagca gcagctttcc cgttatcttt tgttggcgga
    12361 atcggcgttg aaccggcgcg attacgaagc ggcggaagcc aatcttcatg cggcggcgaa
    12421 gatgaatgcc aaccttacgc gcctcgtgcg tctgcaactt cgttacgctt tcgacagggg
    12481 cgacgcgttg caggttctgg caaaaaccga aaaactttcc aaggcgggcg cgttgggcaa
    12541 atcggaaatg gaacggtatc aaaattgggc ataccgccgc cagctggcgg atgctgccga
    12601 tgccgccgct ttgaaaacct gcctgaagcg gattcccgac agcctcaaaa acggggaatt
    12661 gagcgtatcg gttgcggaaa agtacgaacg tttgggactg tatgccgatg cggtcaaatg
    12721 ggtcaaacag cattatccgc acaaccgccg ccccgagctt ttggaagcct ttgtcgaaag
    12781 cgtgcgcttt ttgggcgaac gcgaacagca gaaagccatc gattttgccg atgcttggct
    12841 gaaagaacag cccgataacg cgcttctgct gatgtatctc ggtcggctcg cctacggccg
    12901 caaactttgg ggcaaggcaa aaggctacct tgaagcgagc attgcattaa agccgagtat
    12961 ttccgcgcgt ttggttctgg caaaggtttt tgacgaaacc ggagaaccgc agaaggcgga
    13021 ggcgcagcgc aacttggttt tggcaagcgt tgccgaggaa aaccgacctt ccgccgaaac
    13081 ccattgaaac cttcagacgg catcgttgaa atgccgtctg aaaaccattt cacttgacaa
    13141 acagactgac agaaaacccg tatgactctt ttaaaaaacg ataccttcct ccgcgccctg
    13201 cttaaacagc ccgtcgaata cacgccgatt tggatgatgc gtcaggcggg gcgttatctg
    13261 cccgaataca aagccacacg cgcgaaagcg ggcagcttcc tcgatttgtg caaaaacacc
    13321 gaattggcga ccgaagttac catccaacct ttggaacgtt tcgatttgga cgcggcgatt
    13381 ttgttttccg acatcctgac cgtccccgac gcaatgggct tgggactgta ttttgccgaa
    13441 ggcgaaggcc cgaaattcaa acgcgccctg caacacgagg acgacattgc caaattgcac
    13501 gtccccgata tggaaaaact gcaatacgtt ttcgacgcgg taacttccat ccgtaaagca
    13561 ttggacggcc gcgtaccgct tatcggcttc tccggcagtc cgttcacgct cgcctgttac
    13621 atggtcgaag gcggcagcag caaagaattc cgcaccatca aaaccatgat gtactcgcgc
    13681 cccgatttgc tgcacaaaat cctcgatacc aacgcccaag ccgttaccgc ctacctcaac
    13741 gcccaaatcg acgcgggagc gcaggctgtg cagattttcg acacttgggg cggcgtcttg
    13801 agcgatgcgg cgtttaaaga attcagcctc aaatacatcc gccaaattgt tgccggactg
    13861 aaacgcgaaa gcgaaggcag gcgcgtgcct gtcatcgtgt ttgccaaagg cggcgggctg
    13921 tggctggaaa gcatggcaga aatcggcgca gacgcattgg gcttggactg gacgtgcaac
    13981 atcggcgaag cacgccgccg cgtcggcaag caagtcgccc tgcaaggcaa cttcgacccg
    14041 tttgctctct ttggcacacc cgaatccatc cgcaccgaag tcgcgcgcat cctagccgac
    14101 tacggacacg gcagcggcca cgtcttcaat ctcggacacg gcatcaacca acacgccgac
    14161 cccgaacacg ccaaaatctt agtcgatacc gtacacgagc tgtctcggca gtatcacggc
    14221 gggtaagccg gcaggaaacc gcccgatatg ccgtctgaag ccgagagatg gccggttagg
    14281 gtaaaaataa ggcaatgcgg caatatccgc cgtgtatgga tagtacatga cggcggcggc
    14341 gttgtcgtat tggcgcaatc ccaaccgtcc ctattttcag acggcatttt tgttttcaga
    14401 tgcagggaaa accgatggca aaaacgctta aaacccttta ccaatgcacc gaatgcggcg
    14461 gcacctcccc gaaatggcaa ggcaaatgcc cgcattgcgg cgagtggaac acgcttcagg
    14521 aaagccttgc cgcgcccgag ccgaaaaacg cccgtttcca atcttgggcg gcggacactt
    14581 cgaccgtcca atccctctcc gccgttaccg caaccgaagt gccgcgcaat ccgaccggta
    14641 tgggcgaact cgaccgcgta ttgggcggcg gtttggtcga tggtgcggtc atcctgctcg
    14701 gcggcgaccc cggcatcggc aaatccacgc tgctgttgca aaccatcgcc aaaatggcgc
    14761 aaagccgtaa agtgctatac gtttccggcg aagaatccgc ccaacaagtc gccctgcgcg
    14821 cgcagcgttt ggaactgccg accgacggcg taaaccttct tgccgaaatc cgcatggaag
    14881 cgattcaggc ggccttgaaa cagcatcagc ccgaagttgt cgtcatcgac tctatccaaa
    14941 ccatgtattc cgaccaaatc acgtccgccc ccggctccgt gtcgcaggtg cgcgagtgtg
    15001 ccgcccaact gacgcgcatg gcgaaacaga tgggcatcgc catgatactg gtcggacacg
    15061 tgaccaaaga cggcgcgatt gccggcccgc gcgtgctgga acacatggtt gataccgtgc
    15121 tgtatttcga gggcgaccaa cattccaact accgcatgat acgcgccatc aaaaaccgct
    15181 tcggcgcggc aaacgaactg ggcgtgttcg cgatgacgga aaacggtttg aaaggtgtgt
    15241 ccaacccgtc cgccatcttc ctcgccagct accgcgacga tacgcccggc tcgtgcgttt
    15301 tggttacaca ggaaggcagc cgcccgcttt tggtcgaaat tcaggcattg gtcgatgacg
    15361 cgcacggctt cacgcccaaa cgcctcaccg tcggactgga acaaaaccgt cttgcgatgc
    15421 tgcttgccgt gttaaaccgc cacggcggca tcgcctgttt cgatcaggat gtgttcctca
    15481 acgccgtcgg cggcgtgaaa atcggcgaac cggcggcgga tttggcggtc atcctcgcga
    15541 tgctttccag cttccgcaac cgccctatgc ctgaaaaaac cgtggttttc ggcgaaatcg
    15601 gcttaagcgg cgaagtccgc cccgtcgcac gcgggcaaga gcggctcaaa gaagcggaaa
    15661 aactcggctt caaacgcgcc atcgtcccca aagccaatat gccgcgcaac gccaaagagt
    15721 ttccgaacct gaaaatctac ggcgtttcga gtttgcagga agccatcgat atttgccgcg
    15781 acagcaggga ataaacggaa atgccgtctg aaatcgggtt tcagacggca tttggtttgt
    15841 ggcggattga aacaagaagg cataccggcg acagataaga tttgcggcaa agttgcctgt
    15901 gatgtggcaa aaacacacac gcccgtcatc cccgcaaggg tgggaatccg gaatcgtccg
    15961 tttcggcaat gattgaaaat cacggtaacc caaccgattg gattcccgac ttcgtgggaa
    16021 tgaggggcgt gtgcatttga tttccatccg ccatatgtcg gcgacgggct tattcgccta
    16081 cggttttttg tatcagtttt tcggcgtttg ccaaagtgtt tgccacttcg tcgaaaccga
    16141 tgcggctgcc ggcgatgagg gcgcgcgtat cggtataggc ggcgcgtact ttgccgtccg
    16201 tttcggtaac gaggacgcgt aggggcagtt gcagggcgaa ggcggggtct ttgaccatca
    16261 gcggcgtgcc ggctttgggc gtgccgaaga cgatgacttt tgccggctgc atcgttaagc
    16321 cgtttcggcg ggcggcttcc tgatggtcga tgacggcaaa aatgtccatc cctttgcttt
    16381 ttatggcggt ttcaaggcga ctgacggttt cgtcaaaact gtattttgag gtgagggtat
    16441 gcgtggtcat agcggtttcg ttttgggtgg acggttcgct ggcaggatgt gccgaagcgg
    16501 ttgaaatgca gagtgcggat gcggcaatca gggggagtat gtgtttcatc gtatttcctt
    16561 tttccttttt ggttgaaacg gtagaatcag actttattcg ggaggggtgt aaccctttcc
    16621 aaatcagggc aacacatagg gcggtgcttt atgtgtcgtg aaacatcatt gttccttatc
    16681 ggtttgttta tcaggcttcg gacggggcgg cgcgcgccgc gcccgatgtt gcgccgcgtg
    16741 ccggaacgcc gtatgccgtc tgaaagcctg tcctttcaga cggcattgcg tcatttcatc
    16801 ccttttttga gcaggtcttc ataaccgccg tgattggcaa catttgtata acctgctttt
    16861 ttcagctctt gaagggcggc ttcggcacgc cgtccgctgc ggcagtagag gttgaccggc
    16921 gtgtctttgt cgggcgcggc ttcgtgtatg cggcggacga tttggtcgac ggggatgttg
    16981 accgcgttgt gcaaatgccc ttcgctaaat tcctgttcgg aacggacatc gatccaaacg
    17041 gccggatgtt gcgcggtttg ggcggcggat acgggttttt gcggggctgc ctgcgcggca
    17101 aaggcggctg aggcaatgag tgcggcggta atcaggtgtt tgatattcat agggttttcc
    17161 tgcggttgtt gtccgaaagg acgggaagtt attttatctg ttccaaagcg gcggcatcta
    17221 tgtcccaacg ccaaacgccg ccgcctttgc catccaatcc gcgcaaaaac aggtagcgga
    17281 cggaaacggc ggcgggcggt tgtccgcgca gcttgaagta gcgtgcggcg gcaacggcgt
    17341 aaatcagtgc ctgaaggtaa tagtggtggt gtgcgacggc ttcgtccatt gcctgttgcg
    17401 tgtaggcgga tgcgtccgta ccgaggtggt ttgatttgta gtcgatgacg cagatattgc
    17461 cgtcggggtc ttggcagacc atatcgacaa agccgtttaa aaagccgttg acggtgtgga
    17521 agtcgagcgt ttcggcagcg gcacggcaga cttcgggcag cctgatgtcg tcgcgggcaa
    17581 accagtcgcg caggcgtttg aggctgaagt cttcggtgtg gagggtaaag cccatttcgg
    17641 gacagcggca ctcgggtgag atgtcggaca ggtcgtatgc ccccgtcagc ggcgttttgc
    17701 ggcaggcttc cgccatttcg gcaacggcgg gcagccatat ttcttcaaaa ccgtattttt
    17761 tcagcttgtc ggcaatgagg gtttcctgtc cggcggctgc ttgtccgaat ttgaaatctt
    17821 caagaatttc gtgcaggcac agccccgcct gcgtgccttt cggaaaatcg tgtatcgata
    17881 tgccgtctga agccgtcggc gtttcagacg gcatcgccgg caccgaggtt tcggcggcat
    17941 ccaaggacgg gcaggcatct tcttcgccgc cgtcgggcgt ttgggtatgg cggcttaagg
    18001 cggtaaagct agtgtggcgg acaaatcgga atccgcgttc gggaatgctg tttgcggcaa
    18061 attcggcggt ttgaccggcg ttgctgcgat aggcggcagg gggcggcgca ttttcggtga
    18121 aggcgaaatt tgtgccggaa ggggcgttgt ccgccacgcg ccgccagttg cgtttgagca
    18181 tcgcgatgcc gtctttttca cacgcatagg cacggcggac ggtttcacgg ctgtcttggg
    18241 gcgagccttc aatcaggtag gcgagggggt tgtcggcagt attggtggag tacgcggcgt
    18301 agatgttgag ctgttcctcg gcacgcgtca gcgcgacata aagcaggcgc aggcgttccg
    18361 ccatttcttc atcggcgtat tgtttctgtt cgtcttccga cagttgcgcc tttgccaaca
    18421 gttcggttcg gtttgcgcct tggtggagga tttgccagtc ggacggtccg gtatcttgcg
    18481 cgtcccacgc aaacgggcag tacaccagcg gatactgcaa acctttcgag gcgtgcatgg
    18541 taacgatttt gaccaaatct tcgtcgcttt ccagacggat ggcgcggttg tcgccgctgt
    18601 tgttttcggc aaggctgatt tggtcgccca gccatttgtg cagcgcggcg gggttgcggt
    18661 tttgcgcgtc ttcggcggca agcagttcga gcagttggaa ataattggtc agactgcgcc
    18721 cgttgttccg gcttaagagg cgcgtttcga tgccgtgtgt ttgggaaaat tgctgcatag
    18781 cggcgaaaat gccgtattta ttccagttgt cgagtgcggt tcgggcagat tccgcccaat
    18841 gcaaaatctc gctttcgttt tggttgaagt cgtgcaattg ctgcgcgtca taaccgaata
    18901 tgctgcttgt caggacaaaa cgcagcgttc cggcgcggcg cggttcgagc cagaagccga
    18961 tgagtgcgga cagggcggcg gcttcgggcg aggcgaacac ggattcgcgc gaaagcagga
    19021 cgctttgcac ctgccgtttt ttcagggcgg cggaaaccat caccgcctcg ttgtgcgtgc
    19081 gtaccagcac ggcaatatcg cccgactgca acgggcagcc tttgaaattc agacggcctc
    19141 tggcggcttc gttgagcgcg tgggcgattt cgtcggcgca atagtcggcg gcacggcggc
    19201 gcaaaacgtc tttgttggct ttttcattgt cgttttcgtg cagccaacga acctgtacgg
    19261 caggacgttc gggggacagc ctgctttcgg cacgcgccgc accgacttcc gaatagccga
    19321 tgttttccaa aacgaacggg cgttctttga ggcggaacag cgcgcctatg ctgccgataa
    19381 gcgcggcgtg gctgcggtag ttggtggcga gcgtgtagcg gtgccgcgcg tcttccgccg
    19441 cctgaaggta ggcgtaaatg tccgctccgc gaaagctgta aatcgcctgt ttgggatcgc
    19501 cgacgaggaa cagcggtcgg ttttgggcga tgaaaatctt ttggaagatt tcgtattgca
    19561 gcgggtcggt gtcttggaac tcgtcgatca gcgcggtttc ccagttttcg gcaacggcgc
    19621 gggcgagagt gtcggcgtgc ggattgtcgg tcagcgcggt gtggacatcg agcagcaggt
    19681 cgtcgaaacc gcgttcgcgg cgcgattttt tcatctcggc aaggctgcgg ttgaggtatt
    19741 cgattaaatc cagttgcagc cggatcattg ttgcttcttc cgcttcttcg agtgcgttca
    19801 aatcgcgccc gaagtctgcc agtttctgca attcggcaaa tactgccgca tcgggcgttt
    19861 tgcctttttt cagtccggct tcgagtttgt cggatgcaag tttcaagagt ctgtcgtgtg
    19921 tgtctttgtc cagaaagggc agttgtccgg cggcggattt ttgtgccagt tctttaaaaa
    19981 ggttgccgaa gctgtttttg cggtaactgt tgccgttgag gtcgggatga atgcgccaaa
    20041 agccggcttc cagttctggc agcaggcggc agatggtttg ccatgaggtt tcggcgttgc
    20101 gctgcgcctg tttcaaatcc gcctgcggac ggcggaaatt caggtacggg cgggaaagat
    20161 agcgcgaaat ttgggcaagg acggtttgcg gcacagcttt gcgtttaagc gccaatgcgg
    20221 caagcaccgg atcattgctg acgcgttccc gccaaaaatc ttgcgccggg ataagcaggc
    20281 ggtcgccgtc ttcttcggtc atttcgacat cgaacggtgc ttggcacagg aaggcgtagt
    20341 cgcgcaggat gcgctggcag aagccgtgga tggtatagat ggcggcgttg tcgaattgcc
    20401 cgatggcggc cttgaggcgg acaatcagac gcgtccggcc ctctttttgc aaagcctgtt
    20461 ttaagagttc gggcaggaag gtgtcgcctt cgtggtgttc ggcgcagtag gcggcaatgc
    20521 cgtctgaaag cgtgtcgtct ccaagtttgg caattccttt gctttctaaa acttgtaaca
    20581 catcgtccaa acgcccgcgc aggcgtgttt tcagctcggc ggtggcggct ttggtgaagg
    20641 ttacgaccaa tacgcgttcg acgttttttt gttctaatac gatcaggcgt gtaaacaggg
    20701 cggcaatgcc gtaggttttg ccggtgccgg cagaggcctc aatcaggttg gtgccggaaa
    20761 tggggacggt tagcgggtcg aatgcttgga tgcttgcaga catagtgcgc gctcggaaaa
    20821 cggttggacg gtatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt
//
LOCUS       DAWKOG010000020        20869 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.020, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000020 DAWKOG010000000
VERSION     DAWKOG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20869
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.020"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(25..716)
                     /locus_tag="WJK66_000557"
                     /pseudo
     CDS             complement(25..716)
                     /locus_tag="WJK66_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(975..1694)
                     /locus_tag="WJK66_000558"
     CDS             complement(975..1694)
                     /locus_tag="WJK66_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578311.1"
                     /translation="MLAIYSINILIYNEKMFFRVTLYFVKNFVSFSKTRKSNEMMQKH
                     KCLIDFILTVASQIAYADLPLSLEELLTDKGKFKLESSISYINTERNQSEFANPIYVQ
                     TSATKIATVMAMMITPVMAQNLDSQVFDSQNVKAIQLSQAEMKETQGEFVPIIAAAAF
                     GGALGAWGYHGANLYNHGKLGTAQGAATAAGIGAATGVAATGLAAAAGGGLAGNLAWR
                     PGIHALGFGANAANNAVNSRK"
     gene            complement(1821..1979)
                     /locus_tag="WJK66_000559"
     CDS             complement(1821..1979)
                     /locus_tag="WJK66_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578312.1"
                     /translation="MKTPLPICTLSALAACTLSGQGGSRVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(1976..2818)
                     /locus_tag="WJK66_000560"
     CDS             complement(1976..2818)
                     /locus_tag="WJK66_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ7578313.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPILVDSIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNNPADEHVMLTQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKVQRPQGYRLLGIFEEGKHNAVAVCGFREACTLASGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CAPK"
     gene            2884..3027
                     /locus_tag="WJK66_000561"
     CDS             2884..3027
                     /locus_tag="WJK66_000561"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578314.1"
                     /translation="MPSEKYGIRAVKLCGGKCAIIRAVLPLLRSCSANIRAAWTTYNR
                     IPA"
     gene            3057..3977
                     /gene="pyrB"
                     /locus_tag="WJK66_000562"
     CDS             3057..3977
                     /gene="pyrB"
                     /locus_tag="WJK66_000562"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEZ7578315.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLAHAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            3987..4445
                     /locus_tag="WJK66_000563"
     CDS             3987..4445
                     /locus_tag="WJK66_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HEZ7578316.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            4510..5175
                     /locus_tag="WJK66_000564"
     CDS             4510..5175
                     /locus_tag="WJK66_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578317.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALELGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(5238..6455)
                     /locus_tag="WJK66_000565"
     CDS             complement(5238..6455)
                     /locus_tag="WJK66_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ7578318.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWGRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSVLEQRSKQAGE
                     RPKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFNRSE"
     gene            6597..7142
                     /gene="def"
                     /locus_tag="WJK66_000566"
     CDS             6597..7142
                     /gene="def"
                     /locus_tag="WJK66_000566"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEZ7578319.1"
                     /translation="MKNRRRIRRRKEKIMALLNILQYPDERLHTVAKPVEQVDERIRK
                     LIADMFETMYESRGIGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYE
                     EGCLSVPGIYDTVTRAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVER
                     LSQLKQGRIKTKLKKRQKHTI"
     gene            7229..8155
                     /locus_tag="WJK66_000567"
     CDS             7229..8155
                     /locus_tag="WJK66_000567"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEZ7578320.1"
                     /translation="MKVIFAGTPDFAAAALKAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVAQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQEVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLRKEEARIDWSES
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGTKL"
     gene            8234..9493
                     /gene="rsmB"
                     /locus_tag="WJK66_000568"
     CDS             8234..9493
                     /gene="rsmB"
                     /locus_tag="WJK66_000568"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEZ7578321.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLREREKLASSCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNITAALQSHPPMTLRVNRRHGNAESYLEKLAAEGIPARALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            9459..10058
                     /locus_tag="WJK66_000569"
     CDS             9459..10058
                     /locus_tag="WJK66_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEZ7578322.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVLLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            10058..12175
                     /locus_tag="WJK66_000570"
     CDS             10058..12175
                     /locus_tag="WJK66_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEZ7578323.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAITVQIDLISTASLSGNMGRVLEHY
                     ANGFTQLSLYNAASGKIEKSINPHKLDQPFPDKARWEKIQQAGSVRDLESIGGVLYAQ
                     GWLSAGTHNGRDYALFFRQPVPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATLL
                     IASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTKL
                     FNHMTEQLSIAKEADERNRRREEAAKHYLECVLEGLTTGVVVFDEQGCLKTFNKAAEQ
                     ILGMPLTPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKILL
                     GKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAERL
                     AWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSPSLKLENQDLNALIG
                     DVLALYEAGPCRFAAELAGEPLMVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVKS
                     EAGQDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRIS
                     LSNQDAGGACVRIILPKTVKTYA"
     gene            12168..13445
                     /locus_tag="WJK66_000571"
     CDS             12168..13445
                     /locus_tag="WJK66_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEZ7578324.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QVRPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGALDF
                     LEKPISLQKLLSAVENALKHGAAKIETGPVFDKLGNSAAIQEMNREVGAAVKCASPVL
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQAGIAFIVGKAERCRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPAAFSEDALAVLTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAAAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            13536..14723
                     /gene="dprA"
                     /locus_tag="WJK66_000572"
     CDS             13536..14723
                     /gene="dprA"
                     /locus_tag="WJK66_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEZ7578325.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLMRRFGSAQNALSAPAEQV
                     AALIRHKQALEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLSGNVQLLHKPSAAIVGSRHATPQAMRIAKDFGKSLGGQNIPVVSGMASGIDT
                     AAHQGALEAEGGTIAVWGTGIDRIYPPSNKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLVECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEG
                     AAGGTAVGGILDKMGFDPIHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            14749..15210
                     /locus_tag="WJK66_000573"
     CDS             14749..15210
                     /locus_tag="WJK66_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HEZ7578326.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            15282..17588
                     /gene="topA"
                     /locus_tag="WJK66_000574"
     CDS             15282..17588
                     /gene="topA"
                     /locus_tag="WJK66_000574"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEZ7578327.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGHSKFTAKLAQYNGA
                     KLEQFDLSNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPSRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAESCPKCAWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(17661..18356)
                     /locus_tag="WJK66_000575"
     CDS             complement(17661..18356)
                     /locus_tag="WJK66_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578328.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLMICGYEWGWSKADETAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFAPEMN
                     RILSDFKTTRGFK"
     gene            complement(18376..18927)
                     /locus_tag="WJK66_000576"
     CDS             complement(18376..18927)
                     /locus_tag="WJK66_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578329.1"
                     /translation="MEFLSLHPWWSFFIILTIILSYIGFCAHLHIQNRAVFFYSYRDV
                     TIIFLTPVILIALSYLIKNKEILSACILCITLIAFSWAWIITYRSNTKRFFLSLLIVW
                     GKLLLSTIVWAILAISLGLAVITGNSKRKKYERRDRHAERNEKAFIGALLVGFELSRN
                     LLNLCCLHKDFSTPSKNIEVNRS"
     gene            19046..19424
                     /locus_tag="WJK66_000577"
                     /pseudo
     CDS             19046..19424
                     /locus_tag="WJK66_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            19513..20082
                     /gene="rsmD"
                     /locus_tag="WJK66_000578"
     CDS             19513..20082
                     /gene="rsmD"
                     /locus_tag="WJK66_000578"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEZ7578330.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVCSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGTL
                     PDIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            20205..20456
                     /locus_tag="WJK66_000579"
     CDS             20205..20456
                     /locus_tag="WJK66_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEZ7578331.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEYPETHDELMAKYEKIIQFK"
     gene            20553..20636
                     /locus_tag="WJK66_000580"
     tRNA            20553..20636
                     /locus_tag="WJK66_000580"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20587..20589,aa:Tyr,seq:gta)
     gene            20667..20740
                     /locus_tag="WJK66_000581"
     tRNA            20667..20740
                     /locus_tag="WJK66_000581"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20699..20701,aa:Gly,seq:tcc)
     gene            20749..20823
                     /locus_tag="WJK66_000582"
     tRNA            20749..20823
                     /locus_tag="WJK66_000582"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20781..20783,aa:Thr,seq:ggt)
BASE COUNT         5259 a         5279 c         5658 g         4673 t
ORIGIN      
        1 aataaatggg caaaaatcgg tttattatcg tttttgccgc atttggattt gttctaccgt
       61 aaaacgtgtt tgacgaacgg gattcttatt aaaaaacatc tgatttctaa caaaatcagt
      121 attttttggc acgatggcta aaatttttcc ttccatttcg ccatcacgtg ttttccatgc
      181 gctcaagaat tgtgatttgc tcattgagac gtgccccagc gatggatcag ccagcaaaac
      241 agtttctccg ttaataccgt tcaataccga aaaatggttg tttttacggt attttaaata
      301 cacaattaca ggaattttta gttgtaccaa ctgttcaaat ggcaaagcat aaccttgtgc
      361 ttcaaaaccc agttcgggca ttatgcgttg catatcatca aaagaagcac gcatttgggt
      421 tttatccatt ttgtccaaga tttccgcttc agaataatgt ctgccataaa aattattcag
      481 taacgtggca atcgaagccg cgccgcaaga aaaatccaaa tcttgtttta ctatgccgga
      541 atctcgccgt gctttccaac tccgtacatg gatgttttgg taagaagcgg ggggtcaaca
      601 aacatgggcc aagcaaaaac tatatttggg gcgaaaccaa tcaaagccgc ataatttatc
      661 aatttataaa gattttttat cataatatgt atacgcggaa taaacgcatg aaacataaaa
      721 aaataaaatc atatgcaatt ttattgcatg aataaatatg aataaaatag ataatgatgg
      781 gagtaaatac gccatgtatt ttggaagttt aaatttatta ataataaaat aatttatcgt
      841 agcgcaaata aatcccaaaa ttggaacgat tagaaaaaaa attaatacac ccatcatcat
      901 gcaaactcta tattaataaa tagctaacta attctattgt ggaaattagt tagctaacta
      961 aaagttatta atgattattt tcgagaattg actgcattgt tggcagcatt ggcaccaaaa
     1021 cctagtgcat gaatacccgg tctccatgcc aaattcccag ccaatccgcc tccggcagca
     1081 gcagccagcc ctgttgccgc tactcctgtt gccgcaccga ttcctgccgc agtagccgcg
     1141 ccttgcgcag ttcctaattt accatgatta tacaaattag caccatgata cccccatgca
     1201 cctaatgcac cgccaaaagc agcggctgca ataatgggaa caaattcacc ttgtgtttct
     1261 ttcatttcag cctgtgataa ttgaattgct ttcacatttt ggctgtcaaa aacttggctg
     1321 tctaaatttt gcgccattac aggtgtaatc atcatagcca ttacagttgc aattttcgtt
     1381 gcgctggttt gcacataaat aggattagca aattcgcttt gattgcgttc agtgttgatg
     1441 tagctaatac tgctttctag tttgaattta cccttgtcag tcaataattc ttccaaactt
     1501 aaaggtaagt cagcataagc aatttggctg gcaacagtca aaataaaatc tattagacat
     1561 ttgtgttttt gcatcatttc gtttgatttt ctagtttttg agaatgatac aaagtttttt
     1621 acaaagtaaa gagtcactct gaaaaacatt ttttcattat aaatcaaaat attgatagaa
     1681 taaatagcga gcatcgattc acggtgtact ttagtgcaaa gcgtacaacg cgagcctgaa
     1741 ccaccagccg caacaggaaa agaaagccga tagagtgcaa tgcttgccaa cgtgcaagcg
     1801 agcttgcaag aacgcttggc tcaacgagag caggcaagac agaaagcaga aagcaggata
     1861 ggagcggtaa cgcaaaggtc tcgggctttg atttcgccgt aaaccctgct gccgccttgt
     1921 ccggaaaggg tgcaggcggc gagtgccgac agggtgcaga tggggagggg tgttttcatt
     1981 tgggggcgca acggaagtgg tatgcgcaga tttcaaaacc gtttttgaaa tacaggcggt
     2041 gcgcgtcggc acggtcgtgg ttgacgtgga cgttgaggtg gattttggtt acccctgttt
     2101 ccgcgccgat tttgcggact tcttccagca ggcgcgaggc gtagcctttg cggcggcttt
     2161 gcggcagggt aacgatgtca tcgatgtgga tgtggcggcc gctggcgagg gtgcaggctt
     2221 cgcggaagcc gcagacggcg acggcattgt gtttgccttc ttcaaaaata cccagcaggc
     2281 ggtagccttg ggggcgttgg actttgttga tctgttcggt aaagcggttg atgtcggtca
     2341 gggcggaacg caaaacgctc aaggctgcaa aggcggtggc ggtgtcgtcc gcgccgattt
     2401 cgcgcaaaac gtaggatgcg cccgaggcgg tctgttcctg tgctttctcg gcggcgtgtt
     2461 tttcttcgat tgcctgtgtc agcatgacgt gttcgtcggc agggttgttt tgtccgccct
     2521 gttcgcgttc ttcgagcagg gctttgcagt cgatgacgcg caggtcgttg tcggcggcaa
     2581 agtccatcag gaagcggaac atttggggat tgtcggtttc cagtttttta tcgacggcga
     2641 cgcagcggat gctgtccacc agtatggggc gcacccattt cgaataggaa agcctgtggt
     2701 ctttggtgaa cgaggacaga atgccgcaca ggcgttccgc ccagtcgctg ggacggaaaa
     2761 tcttgccgga actcgttgtg ccgtggatga cgacttcgta ggggttgcag actaacatgg
     2821 cggcttcctg aaaagaaatg tctggctcga ttatacctta tgcttatgca ggcgtgtttg
     2881 gatatgccgt ctgaaaagta cgggattcgt gcggtaaaac tttgcggcgg caaatgtgcg
     2941 ataatacgcg ccgtattgcc gcttttgcga agctgttccg caaacatacg ggcggcgtgg
     3001 acgacgtata accggatacc cgcctgacgc gggtttttta cggaaggggg gcaaaaatgc
     3061 ctaatccgct ttacagacag catatcatct ccatttcgga tttgtcgcgc gaacagttgg
     3121 aatgcctgct tcagacggca ttgaagctga aggcgcatcc gcgcggcgat ttgttggaag
     3181 gcaaacttat cggttcgtgc ttctttgaac cgtccacgcg cacgaggctg tcgtttgaaa
     3241 cggcggtgca gcggctgggc ggcaaggtca tcggcttttc ggacggcgcg aataccagtg
     3301 ccaaaaaagg cgagacgctt gccgataccg cccgcatcat ttccggctac accgacgcaa
     3361 tcatccaacg ccaccccaaa gacggcgcgg cgcgcgtggc agcggagttt tcgcgcgtcc
     3421 ccgtcatcaa cgccggcgac ggcaccaacc agcaccccag ccagaccttg ttggatctgg
     3481 taacgattta tgaaacgcag gggcgtttgg acaagctcaa aatcgccatg gcgggcgact
     3541 tgaaatacgg gcgcaccgtg cattcgcttt gtcaggcgtt gaaacgctgg ggctgcgaat
     3601 ttgcctttgt ttcgccgccc agcctcgcga tgcccgacta cattaccgaa gagttggacg
     3661 aagccggctg ccgataccgt atcctcggca gtttggaaga ggcggcggaa tgggcggaca
     3721 tcctgtatat gacccgcgtc cagcgcgaac gtttcgatga acaggaattt gccaaaatcc
     3781 aaggcaaatt caacctcgaa gcgtctatgc tcgcccacgc caaaccgaac ctgcgcgtgc
     3841 tgcacccttt gccgcgcgtg gacgaaatcc atcccgatgt cgatgccacg ccgcatgcct
     3901 attatttcga gcaggcaacc aacggcgttt atgcgcgtat ggcgatattg tcgctggtgt
     3961 tgaacgaaga agtgtgagga accgatatgg aaaccccgaa actcagtgtc gaagccattg
     4021 aaaaaggtac ggttatcgac catattcccg ccggcagggg gctgaccatc ctgcgccagt
     4081 tcaaactttt gcactacggc aacgcagtaa ccgtgggctt caacctgccc agcaaaaccc
     4141 aaggcagcaa agacatcatc aaaatcaaag gcgtgtgctt ggacgacaaa gccgccgacc
     4201 gccttgccct gtttgcaccc gaagcagtgg tcaacaccat cgacaatttc aaggttgtgc
     4261 agaaacggca tttgaacctg cccgacgaaa tcgccgaagt gttccgctgt ccgaacacga
     4321 attgcgccgg ccacggcgag ccggtcaaaa gccggtttta tgttaaaaaa cacaacgggc
     4381 agacgcggct gaaatgccat tactgcgaaa aaacctacag ccgggattcg gtggcggaag
     4441 cctgacggat tcccttaaac cgagcgggcg gcatttcgtc tgccgcttgt tttgccaatc
     4501 tgaaatggaa tgatgatgca cgcttctgtc caaagccgtt tcgcaccgat actttatgtt
     4561 ttgattttct ttgccggttt tttgaccgcg caaatctggt tcaatcagaa agcctatact
     4621 gaagagctgc ctccgcttct gtccgcattg tccgccgtcg cgctggtgtg gctggcgtgg
     4681 gcgttcgtgt cggcgcgttc aaaggctaag gcggaaaagt tctaccgcga aaaaatgata
     4741 cagaacgaaa gcatacaccc cgtcctgcac gcttctttgc aacacttgga acacaagccg
     4801 caaatgctcg ccctgctggt caaaaaccac ggcaaaggta tggcggagca ggtcaggttc
     4861 aaggcagaag tgctgcccga cgacgaagac gcgcgcacga ttgccgccga gttggcaaaa
     4921 atggatatgt tcgcattggg gacggacgcg gtcgcctcgg gcgaaaccta tggacgcgtg
     4981 ttcgccgata ttttcgagtt gtcggcggct ttggaagggc gcgcgttcaa aggaatgttg
     5041 aaactgacgg cggaatataa aaacatcttc ggcgatgcct gccgttcgga aacggcgttg
     5101 gagttgggcg cgctcaatca ggcgttgcag gagatttcaa aaacatcgga aaagtccaaa
     5161 cggatatttt attgaagatg gaaaaatgcc gtctgaaacg gaaggtgttt cagacggcat
     5221 ttttgtcgga tgattaatta ttcggagcgg ttgaagccaa acttcacgcg gctgcggccc
     5281 tgatccggta tattgtccaa atcgcgtccc ggattggcgg cggtgtcgcc tacggaaata
     5341 tcggagatgt tttccaaaat gatggcggac gacaggtgtt cggaggtgcg gtaaaccatt
     5401 gccaagccca cttcttcggc aggagtggaa atcagctcga cggtatccct gcttttgaaa
     5461 ttgttggaga ggtcgacctg catcgttttc ttgcgtttgt agaggctcaa aaccgtgcct
     5521 ttgtccaaac cgtccgcctc gcctttgtcg atggtgatgg tttgaaactg gccggcaatc
     5581 cttgtgcctt caaacacgga aacgatttta gcctgaaccg ggcgggacgg ttcgtgcggc
     5641 atcatgttga agcggtcggt gtcttccggc attttcatca ggtagtcgcc ctgctgtatt
     5701 tcggaaatgg cggtttcgac caccagcggc tgtatcgaag gggtgcgcag cggggtaatc
     5761 aaaggatggg tgcgggtatg gtattcgttg tctttcggcc gttctccagc ctgtttcgag
     5821 cgttgttcga ggacagagtc ggtatagtcg agggagcgca cgatgccgct gaatgcgact
     5881 tcctgcccga ggaatttacc cgtatccgga tcggtgatgt ttttattgat tcggtaggtc
     5941 aggtagcgac ccggctcttt caggcctttg gtgtaaaccc tggtgccttt ggtgtacagc
     6001 agcctgcctt ccgggcccga gagcaggcgc ggcgcggcag cggtttcttt gcgggaaacg
     6061 atttgcgggt gccgcataaa gatgcggtag aagttgacat cgatggcggg aataccgtat
     6121 ccggacactt ccttatccgg actcattttg acgacgggga tgccgtctgt ctgttccaag
     6181 ccgaggcgcg gttcgccgtc aacgtggcgc aacaccaata cctggtccgg ataaatcagg
     6241 tcgggattgt ggatttgatc ccggttcgcg tcccacaggc ggccccattg ccacgggctg
     6301 tacaggtatt tgcccgaaat gccccacagg gtgtcgccct gtttgaccgt gtagcgttcc
     6361 ggcgcgttcg ggcgcacctc caaatttgcc gccaaagttt gtgttgagaa tgccatacct
     6421 gccgcgcaga gcagggttat aatacgacgt tgcataaccg ttccccttat ctgataaatt
     6481 tcggtttgtc ttgcttgatt gggttggaaa aagcggcggc agcccctcgg gatgtgccgc
     6541 gtgataaaaa atgttccgca ttttaacatc gaattatccg caccatcacg gtaattatga
     6601 aaaacaggcg gcgtatccgc cgaaggaaag agaaaattat ggctttattg aatatcttgc
     6661 aatatcccga cgagcgtctg cacacggtgg caaagcctgt cgaacaagtc gacgagcgca
     6721 tccggaagct gattgccgat atgtttgaaa cgatgtacga atcgcgcggc atcgggctgg
     6781 cggcgacgca ggtcgatgtg cacgagcgcg tggtcgtgat ggatttgacc gaagaccgca
     6841 gcgaaccgcg cgtgttcatc aaccccgtca tcgttgaaaa agacggcgaa accacttacg
     6901 aagagggctg cctgtccgtg ccgggcattt acgacaccgt aacccgcgcc gaacgcgtca
     6961 aggtcgaggc tttgaacgaa aaaggcgaaa agttcacgct ggaggcggac ggcttgctgg
     7021 cgatttgcgt gcagcacgag ttggaccacc tgatgggcat cgtgtttgtc gaacgcctgt
     7081 cgcagctcaa gcagggacgc atcaaaacca agctgaaaaa acgtcagaaa catacgattt
     7141 gacccttttg ccgtgccgtc tgaacgctgc aaagttttca gacggcacgg tcttgtccga
     7201 caaccttacg catacgcagg aatatgctat gaaagtcatc tttgccggca cgcccgattt
     7261 tgccgccgcc gccttaaaag ccgttgccgc cgccggtttt gaaatcccgc tggtgctgac
     7321 ccagcccgac cgtccgaaag ggcgcggtat gcaactgacc gccccgcccg tcaaacaagc
     7381 cgcgctggaa ctcggtttgc gcgtggcaca gcccgaaaaa ctgcgtaaca acgccgaagc
     7441 cctgcaaatg ctcaaagagg tcgaggcaga cgtaatggtg gttgccgcct acggtttgat
     7501 tctgccgcag gaagtgttgg atacgccgaa acacggctgc ctcaacatcc acgcttcgct
     7561 gttaccccgt tggcgtggcg cggcgccgat tcaacgcgcg attgaagccg gcgatgccga
     7621 gacaggcgtg tgtattatgc agatggacat cggtttggac accggcgatg tggtcagcga
     7681 acaccgctac gccatccaac cgaccgatac cgccaacgaa gtccacgacg cgctgatgga
     7741 aatcggtgcg gcggcggttg ttgccgattt gcaacagctt caaagcaaag gccgtctgaa
     7801 cgcggtcaaa cagcccgaag aaggtgttac ttacgcccaa aaattgcgca aagaagaggc
     7861 gcgtatcgat tggagcgaaa gcgcggcggt tatcgaacgc aaaatccgcg ccttcaaccc
     7921 cgtgcctgcc gcgtgggttg agtatcaggg caagccgatg aaaatccggc gggcggaagt
     7981 ggtggcgcaa caaggcgcgg caggcgaagt gttgtcctgt tcggcggacg gtttggtcgt
     8041 tgcctgcggc gaaaacgcgc tgaagattac cgaattgcag cctgccggcg gcaggcgtat
     8101 gaatatcgcg gcgtttgcag caggacggca tatcgaagca gggacgaagc tgtaaacctt
     8161 tttcagacgg catttgatcc gcaaacggga atgccgcctg aaaccatcag ttgaagaaag
     8221 cgaatcacat aatatgagta tggcacttgc ccaaaaactt gccgccgaca gcattgcggc
     8281 ggttgccgaa ggacgcaacc ttcaggacgt gttggcgcaa atccgcaccg cgcatcccga
     8341 ccttacggcg caggaaaacg gcgcgttgca ggacatcgcc tacggctgcc agcgttattt
     8401 gggcagtttg aaacatatgc tcgcgcagat gctgaaaaag ccgattgaca acccgcagct
     8461 cgaaagcctg cttttggcgg cgttgtacca actgcattac acgcgcaacg cgccgcacgc
     8521 cgtggtcaat gaggcggtgg aaagcatcgc gaaaatcggg cgcgggcagt accgttcgtt
     8581 tgccaacgcg gttttgcgcc gttttttgcg tgaacgtgaa aaattggcgt cttcctgcaa
     8641 aaaagacgat gtggcaaaac acaacctgcc gctgtggtgg gtggcttact tgaaaaacca
     8701 ttatccgaaa cactggcaca acatcaccgc cgcgctgcaa tcgcatccgc cgatgacgtt
     8761 gcgcgtcaac cgccgacacg gcaatgccga aagctatttg gaaaaactgg ctgcggaagg
     8821 tattccggcc agggcgttgg acgaatatgc ggttacgttg gaagaagccg tgccggtaaa
     8881 ccgtctgccc ggtttttcag acggcattgt ttcggtacag gacttcggcg cgcagcaggc
     8941 ggcgtatctc cttaacccga aagacggcga acggattttg gacgcgtgcg ccgcgccggg
     9001 cggcaagacg gggcatattt tggaactggc ggattgccgc gttaccgcct tggacatcga
     9061 tgcaggccgt ctgaagcgtg tggaggacaa tatcgcccgt ttgggctttc agacggcatc
     9121 ggcggcgtgt gccgatgcgc gggatttggc ggcgtggtat gatggaaagc ctttcgacgc
     9181 aatccttgcc gacgtgccat gcaccgcttc cggcgtggcg cggcgcaatc ccgacgtgaa
     9241 atggctgcgc cgtccgaccg acgcgctcaa aaccgcccgc cagcaggaag ccctgctgga
     9301 cgcactttgg cagacgctga aacagggcgg cagaatgctg cttgccacct gttccgtgtt
     9361 cgtcgaagaa aacgacgggc aattgcaaaa attcctcaac cgccatgccg atgccgaacc
     9421 gatcgaatcg cgggtgcttt taccgaacaa acaccaagat ggcttttatt acgcgcttat
     9481 tcaaaagcat taaacaatgg cttgtgctgc tgccgatgct ttccgttttg ccggacgcgg
     9541 cggcggaggg gatagatgtg agccgcgccg aagcgaggat aaccgacggc gggcagcttt
     9601 ccatcagcag ccgcttccaa accgagctgc ccgaccagct ccaacaggcg ttgcgccggg
     9661 gcgtgctgct caactttacc ttaagctggc agctttccgc cccgataatc gcttcttatc
     9721 ggtttaaatt ggggcaactg attggcgatg acgacaatat tgactacaaa ctgagtttcc
     9781 atccgctgac caaccgctac cgcgttaccg tcggcgcgtt ttcgacagac tacgacacct
     9841 tggatgcggc attgcgcgcg accggcgcgg ttgccaactg gaaagtcctg aacaaaggcg
     9901 cgctgtccgg tgcggaagca ggggaaacca aggcggaaat ccgcctgacg ctgtccactt
     9961 caaaactgcc caagccattt caaatcaatg cattgacttc tcaaaactgg catttggatt
    10021 cgggttggaa acctctaaac atcatcggga acaaataatg cgccgttttc taccgatcgc
    10081 agccatatgc gccgtcgtcc tgttgtacgg actgacggcg gcaaccggca gcaccagttc
    10141 gctggcggat tatttctggt ggatagttgc gttcagcgca atgctgctgc tggtgttgtc
    10201 cgccgttttg gcacgttatg tcatattgct gttgaaagac aggcgcgacg gcgtattcgg
    10261 ttcgcagatt gccaaacgcc tttctgggat gtttacgctg gttgccgtac tgcccggcgt
    10321 gtttctgttc ggcgtttccg cacagttcat caacggtacg attaattcgt ggttcggcaa
    10381 cgatacccac gaggcgcttg aacgcagcct caatttgagc aagtccgcat tgaatctggc
    10441 ggcagacaac gcccttggca acgccatcac cgtgcagata gacctcatca gcaccgcctc
    10501 cctatccggc aatatgggca gggtgctgga acattatgcc aacgggttta cacagttgtc
    10561 cctttacaat gccgcaagcg gcaaaatcga aaaaagcatc aacccgcaca agctcgatca
    10621 gccgtttccg gataaggcgc gttgggaaaa aatccaacag gcgggttcgg tcagggattt
    10681 ggaaagcata ggcggcgtat tgtacgcgca gggctggctg tcggcgggta cgcacaacgg
    10741 gcgcgattac gccttgtttt tccgtcagcc ggttcccaaa ggcgtggcag aggatgccgt
    10801 cttaatcgaa aaggcaaggg cgaaatatgc tgagttgagt tacagcaaaa aaggtttgca
    10861 gacctttttc ctggcaaccc tgctgattgc ctcgctgctg tcgatttttc ttgcactggt
    10921 catggcactg tatttcgccc gccgtttcgt cgaacccgtc ctatcgcttg ccgagggggc
    10981 gaaggcggtg gcgcaaggcg atttcagcca aacccgtcct gttttacgca atgacgagtt
    11041 cgggcgtttg accaagttgt tcaaccacat gaccgagcag ctttccatcg ccaaagaagc
    11101 agacgagcgc aaccgccggc gcgaggaagc cgccaaacat tatcttgaat gcgtgttgga
    11161 ggggctgacc acgggcgtgg tggtgtttga cgaacaaggc tgtctgaaaa ccttcaacaa
    11221 agcggcggaa cagattttgg ggatgccgct tacccccctg tggggcagca gccggcacgg
    11281 ttggcacggc gtttcggcgc agcagtccct gcttgccgaa gtgtttgccg ccatcggcgc
    11341 agcggcaggt acggacaaac cggtccatgt gaaatatgcc gcgccggacg atgccaaaat
    11401 cctgctgggc aaggcaaccg tcctgcccga agacaacggc aacggcgtgg taatggtgat
    11461 tgacgacatc accgttttga tacacgcgca aaaagaagcc gcgtggggcg aagtggcaaa
    11521 acggctggca cacgaaatcc gcaatccgct cacgcccatc cagctttccg ccgaacgtct
    11581 ggcgtggaaa ttgggcggga agctggatga gcaggatgca caaatcctga cgcgttcgac
    11641 cgacaccatc gtcaaacagg tggcggcatt gaaggaaatg gtcgaagcat tccgcaatta
    11701 tgcgcgttcc ccttcgctca aattggaaaa tcaggatttg aacgccttaa tcggcgatgt
    11761 gttggcattg tatgaagccg gtccgtgccg gtttgcggcg gagcttgccg gcgaaccgct
    11821 gatggtggcg gcggatacga ccgccatgcg gcaggtgctg cacaatattt tcaaaaatgc
    11881 cgccgaagcg gcggaagaag ccgatgtacc cgaagtcagg gtaaaatcgg aagcggggca
    11941 ggacggtcgg attgtcctga cggtttgcga caacggcaaa gggttcggca gggaaatgct
    12001 gcacaacgcc ttcgagccgt atgtaacgga caaaccggcg ggaacgggat tgggtctgcc
    12061 tgtggtgaaa aaaatcattg aagaacacgg cggccgcatc agcctgagca atcaggatgc
    12121 gggtggggcg tgtgtcagaa tcatcttgcc aaaaacggta aaaacttatg cgtagcagcg
    12181 atattttaat tgtagacgac gaaatcggca tccgcgacct gctgtcggaa atcctgcagg
    12241 acgaaggtta ttcggtcgca ttggcggaaa acgccgaaga ggcgcgcaag ctgcgccatc
    12301 aggtacgccc ggcgatggtg ttgctggata tttggatgcc cgattgcgac ggcatcaccc
    12361 ttttgaagga gtgggcgaaa aacgggcagc tcaatatgcc ggtggtgatg atgagcgggc
    12421 atgccagcat cgataccgcc gtagaagcga ccaaaatcgg cgcgctcgac tttttggaaa
    12481 aaccgatttc cctgcaaaag ctgctgtctg ccgtcgaaaa cgcgttgaaa cacggtgcgg
    12541 cgaaaatcga aacggggcct gtattcgaca agctgggcaa cagtgcggcg attcaggaaa
    12601 tgaaccgtga ggtaggggct gcggtgaaat gtgcctctcc cgtacttttg acgggcgagg
    12661 caggttcgcc gtttgaaacg gtggcacgct atttccataa aaacggtacg ccgtgggtca
    12721 gcccggcaag ggtcgaatat ctgatcgata tgccgatgga actgttgcag aaggcggagg
    12781 gcggcgtttt gtatgtcggc gacatagccc aatacagccg caacatccaa gccggcattg
    12841 cctttattgt cggcaaggca gaacgctgcc gcgtcagggt ggtcgcatcg ggcagcaggg
    12901 cggcaggttc agacggcatt gcctgcgagg aaaagctggc ggaactgctg tcggaatcgg
    12961 tcgtccgtat tccgccgctg cgtatgcagc atgaagatat tcccttcctg atacagggga
    13021 ttgcctgcaa tgtggcggaa agccaaaaga ttgcgcccgc cgcattcagt gaagatgcgc
    13081 ttgccgtgct gacccgttac gactggccgg gaaatttcga ccaactgcaa agcgtcgttg
    13141 caacgctgtt gttggaggcg gacggacagg aaatcggcgc ggcggcggtt tcttcccttt
    13201 tggggcagaa tgtgcctgcc gagggagcag aagatatggt gggcgggttt aatttcaacc
    13261 tgcccctgcg cgaattgagg gaggaggtgg agcggcgtta tttcgagtac cacatcgccc
    13321 aagaaggtca gaatatgagc aaagtggcgc agaaagtcgg tttggaacgc acgcaccttt
    13381 accgcaaact caaacagctc ggcatcggcg tttcgcgccg ggcgggggaa aaaaccgaag
    13441 aataggcccg gacggccggt tttccggctg cgggcttttg ttttcagacg gcatttggtg
    13501 caaatgccgt ctgaaatcgt aaggggacgg attttatgac agaggacgaa cgtttcgcgt
    13561 ggctgcaatt ggcgtttacg ccctatatcg gcgcggaaag tttcctgctg ctgatgcgcc
    13621 gtttcggcag cgcgcaaaat gccctgtccg caccggcgga acaggtggcg gcactgatac
    13681 ggcacaaaca ggcgcttgag gcttggcgca atgcggaaaa acgcgctctg gcgcggcagg
    13741 cggcagaagc ggcattggaa tgggaaatgc gggacggatg ccgtctgatg ctgcttcagg
    13801 atgaagattt tcccgaaatg ctgacgcagg ggctgaccgc gccaccggtt ttgtttttga
    13861 gcggcaacgt acaactgctg cacaaacctt ccgccgccat tgtcggcagc cgtcatgcca
    13921 cgccgcaggc aatgcggatt gccaaagatt tcggcaagtc gttgggcggg caaaatatcc
    13981 ccgttgtgtc gggtatggct tccggtatcg atactgccgc ccatcagggc gcactggagg
    14041 cagaaggcgg caccatcgcc gtgtggggga cgggtataga ccgcatttac ccgccgtcca
    14101 ataaaaacct tgcctatgaa atcgccgaaa aaggattgat tgtcagcgag tttcccatcg
    14161 gtacgcgccc gtatgccggc aattttccgc gccgcaaccg cctgattgct gccctgtcgc
    14221 aagtaacgct ggtggttgaa gccgccttgg aatccggttc gctgattacc gccggcttgg
    14281 cggcagaaat ggggcgcgaa gtgatggcgg tacccggttc gatagacaat ccgcacagca
    14341 aaggctgcca caaactgatt aaagacggcg caaaattggt ggaatgcctg gacgacatcc
    14401 tgaacgaatg cccggggcta ttgcaaaata cgggtgcttc atcatattct ataaataagg
    14461 atacgcccga cacgggccgc cgcgccgttc agacggcata cgccccgccg cctgccgcaa
    14521 aaatgccgtc tgaaggggcg gcaggcggca cagccgtcgg cggcatactg gataaaatgg
    14581 gtttcgaccc catccatccc gacgtgcttg ccggacagtt ggctatgcct gccgcagatt
    14641 tgtatgccgc actgttggaa ttggaattgg acggcagcgt tgccgcaatg cccggcggca
    14701 gataccagcg tatccgaact tgaacgcact ttatattaag gaacacgaat gaccgaagtc
    14761 atcgcctacc tcatcgaaca tttccaagat ttcgatacct gtccgccgcc cgaagacttg
    14821 ggtatgctgc ttgaagaagc gggtttcgat acgatggaaa tcggcaacac cctgatgatg
    14881 atggaagtat tgctcaacag ctccgaattt tccgccgaac ctgccgacag cggcgcattg
    14941 cgcgtgtaca gcaaagaaga aaccgacaac ctgccgcagg aagtgatggg gctgatgcag
    15001 tatctgattg aagaaaaagc cgtcagctgc gaacagcggg aaatcatcat ccacgcgctg
    15061 atgcacatcc ccggcgacga aattaccgta gataccgcca aagtgctgac cctgctgctt
    15121 ttatgggcaa acaagagcga gctgcccgtg ttggtcggcg acgagctgat gagcgcgctt
    15181 ttactcgaca acaaacccac gatgaactga agcggcttca gacggcccgc ccgaggccgt
    15241 ctgaaacgcc ggcatcaaaa ccaccatcca gagaacgaca aatggcgaaa aacctattaa
    15301 tcgtcgaatc cccgtccaaa gccaaaaccc tgaaaaaata tttgggcggc gattttgaaa
    15361 tccttgcatc ctacggacac gtccgcgacc tcgtccccaa aagcggcgcg gtcgatcccg
    15421 acaacggctt tgcgatgaaa taccaactca tcagccgcaa cggcaaacac gtcgatgcca
    15481 tcgtcgccgg tgccaaagaa gctgaaaaca tctacctcgc caccgacccg gatagggaag
    15541 gcgaagccat ttcctggcat cttttggaaa tcctcaaatc caaacgcggc ttgaaaaaca
    15601 tcaagccgca gcgtgtcgtg ttccacgaaa tcaccaaaaa cgccgtgctc gatgccgttg
    15661 cccatccgcg cgaaatcgaa atggacttgg tcgatgcaca acaagcccgc cgtgctttgg
    15721 actatttggt cggctttaac ctttcgccat tgttatggaa aaaaatccgc cgaggtttga
    15781 gcgcggggcg tgtgcaaagc cctgctttgc gcctgatttg tgagcgcgaa aacgaaatcc
    15841 gcgcgtttga agcgcaggaa tattggacgg tgcatctcga cagccacaaa ggccacagca
    15901 agttcaccgc caaactcgcc caatacaacg gcgcgaaact cgaacaattc gacctgtcga
    15961 acgaagccgc gcaagccgac gtattgaaag aactcgaagg caaagaagcc gtcgttaccg
    16021 ccattgaaaa gaaaaagcgc agccgcaatc ctgccgcgcc gttcaccact tctaccatgc
    16081 agcaggatgc cgtgcgcaaa ctcggcttca ccaccgaccg caccatgcgt accgcccaac
    16141 agctttatga aggtatagac gtagggcagg gcgcgatcgg tctgattacc tatatgcgta
    16201 ccgacagcgt gaatttggcg gatgaagcgt taaccgaaat ccgccattac attgaaaaca
    16261 aaatcggcaa agaatatctg ccgagtgccg ccaaacaata caaaaccaaa tccaaaaacg
    16321 cccaagaagc ccacgaagcc atccgtccga cttccgtgta ccgcacgccc gaaagcgtca
    16381 aacccttcct gagcgcagac cagttcaaac tctatcaaat gatttggcag cgtaccgtcg
    16441 cctgtcagat gacgcctgcc aaattcgacc aaaccaccgt cgatattacc gtcggcaaag
    16501 gcgtattccg cgtaaccgga caagtgcaaa ccttcgcagg cttcttgagc gtttatgaag
    16561 aaagcagcga cgatgaagaa ggcgaagaca gcaaaaaact gcccgaaatg agcgaaggcg
    16621 ataaattgcc cgtggacaaa ctctacggcg aacaacactt taccactccg ccgtcgcgct
    16681 acaacgaagc cacgctggtt aaagccctcg aagaatacgg catcggccgc ccctcgacct
    16741 acgccagcat catctccacg ctcaaagacc gcgaatacgt tacccttgag caaaaacgct
    16801 tcatgcccac cgacacaggc gacatcgtca acaaattcct gaccgaacac ttcgcccaat
    16861 acgtcgatta ccacttcacc gccaaactcg aagaccagct tgacgaaatt gccgacggca
    16921 aacgccaatg gattcccttg atggacaaat tctggaaacc gttcatcaaa caagtggaag
    16981 aaaaagaagg catcgaacgc gccaaattta ccacgcagga acttgatgaa acctgcccga
    17041 aatgcggcga acacaaactg caaatcaaat tcggcaaaat gggtcgtttt gttgcgtgtg
    17101 ccggttatcc cgagtgcagc tacacgcgca atgtcaacga aaccgccgaa gaggccgccg
    17161 agcgcatcgc caaagccgaa gccgaacagg ccgaactcga cggacgcgaa tgtcctaaat
    17221 gtggtggtcg cctagtgtac aaatacagcc gaaccggcag caaattcatc ggctgcgcca
    17281 actatccgaa atgcaaacac gtcgagccgc tggaaaaacc gaaagatacc ggcgtccagt
    17341 gtccgcaatg caaaaaaggc aacctcgtcg agcgcaaatc ccgctacggc aaactgtttt
    17401 acagttgcag cacctatccc gactgtaact acgccacatg gaacccaccc gttgcagaaa
    17461 gctgtccaaa atgcgcatgg ccggttttaa ccatcaaaac caccaaacgc tggggcgtcg
    17521 aaaaagtctg cccgcaaaaa gaatgcggct ggaaagaaca gattgaaccg cctgcgccga
    17581 aagagtgaga ttaggttggt ttgaaagaga aaaggtcgtc tgaaaaattt tcagacgacc
    17641 tttgcttttc tgtgattggt ttatttgaat ccgcgtgttg ttttaaagtc cgataaaatc
    17701 cggttcattt caggcgcaaa caaggcgatg taatcgtaag atagaccgcg actggcactg
    17761 ggatggggaa agcagacgac ttcgcaatct tcaaacgatt ggaatttgac attgaaacgt
    17821 gtaccgtcaa attctttttg caccgtctcc agcggtttgg tctgcttacc gaccaactgc
    17881 tcgaagcgtg gcagtacatt ttggttgttc agaaaatccg ccaacctgct gcccatgaag
    17941 aggatgactt tcggacgcag tttttcgatg tggtagagaa aattatcgat gtgctcgggt
    18001 tgtgtgaact tgtcgggatt gtcgatagtg ttgccctgtg tagcagccca gttggtttga
    18061 accagggatt tttcaaatgc accgcccaat ccattttcgt ctaaggggtg tccccacatt
    18121 tcaaaccaat tttttatcgt attgtcgtaa cgccactttt ttgcctgctc tccgaaatag
    18181 agggatttgt ttgcaaatgt atggtcgatt ttgttttcag ggagtttgta ttcacctgct
    18241 acataagcag tctcatcggc tttactccaa ccccattcat agccacaaat cattaagcca
    18301 tgtttgtcgt tgtagccttt gaacaggctg ttgctcaaat tcaaatcctt catcatgaac
    18361 tcttcctttt aaaatttaag agcgattgac ttcaatgttt ttagatgggg tggaaaaatc
    18421 cttgtgtagg caacataaat tcaataaatt tcttgataat tcgaaaccta ctaatagcgc
    18481 acctataaaa gctttttcat tacgttcagc atgacggtca cgtcgttcat attttttacg
    18541 cttgctgttc cctgttatta cagctaagcc aagtgatatg gcgagaattg cccaaacaat
    18601 agtacttaat aacaattttc cccatactat caataaggaa agaaaaaacc ttttagtatt
    18661 agatcgatag gttataatcc atgcccatga aaatgcaata agagttatac ataagataca
    18721 agctgacagg atttctttat ttttaattaa ataacttaga gcgatgagga tgacaggtgt
    18781 caagaaaata atagttacat cccgatagct ataaaagaaa actgccctat tttgaatgtg
    18841 gagatgtgca cagaatccaa tatagctaag gataatagtt aatataataa aaaaagacca
    18901 ccaagggtga agagatagga attccatgtt ttcccttttt ttgtaaaaag gaaaaaatct
    18961 tatctaatag ttaattgcct aaccagccag aagaagttta aaatctatcc caataattca
    19021 accatctata cagaaagttc agcttatgga aacccacgaa aaaatccgcc tgatgcgcga
    19081 attgaataaa tggtcccagg aggatatggc ggaaaagctg gcgatgtcgg caggagggta
    19141 tgccaaaatc gaacggggcg aaacgcagtt gaatatcccg cgtttggagc agttggctca
    19201 gattttcaaa atcgatatgt gggacttgct caaatcgggc ggtggtggga tggtgtttca
    19261 gattaatgaa ggtgatagtg gtggcgatat ttgcgttgta tgcgtcgggt gatgtttcga
    19321 tgaaaataga atttttaaaa atggagttga aacactgcaa agaaatgttg gaacaaaaag
    19381 acaaagaaat cgagctgctc cgcaagctga ccgaaaccgt ttaaacagat atgccgtctg
    19441 aaaaaagttt tcagacggca tattctttga caggtcttgt ataataccgt ttgaacttac
    19501 aggtttttga ttatggcggc aggcaaacat accaaacaca gcaaccgggt acgcattatc
    19561 ggcgggcaat gccggggcag gaaattgagt ttcacatccg ccgacggact gcgtccgaca
    19621 cccgacagcg tgcgtgaaaa gctgtttaac tggctgggac aggatttgac gggtaaaacg
    19681 gttttggatc tcttcggagg cagcggcgca ctcggtatag aagccgcttc gcgcaacgcc
    19741 aaacgcgtgc tgatttcgga taacaaccgc caaaccgtgc agaccttgca gaaaaacagt
    19801 cgcgaactgg gtttggggca ggtgcaaatc gtctgttcag acggcatcgc atatttgaag
    19861 accgtatccg aacagtttga tgttgtcttt ctcgacccgc cgtttgcatg gcaggactgg
    19921 caaatcctgt ttgatgcctt gaagccgtgc ctgaaccccc gggcattcgt ctatctcgag
    19981 gcgggtacgc tgccggatat tcccgattgg ctgacggaat atagagaggg gaaatcgggg
    20041 cagagtacat ttgaattaag ggttttccaa gtggctgaat aatatgcgct ttgataatca
    20101 tttccgagtt gtaaacattc gtttgcaacc gtccggttca aaaaaacctt gtgctataat
    20161 ccgcgcccgc ccggttttga taatttagtg gaaaaggaaa agaaatgtcg ctttttatta
    20221 ccgacgagtg catcaactgc gacgtatgcg aacccgaatg ccccaatgat gccatttccc
    20281 aaggcgagga aatttacgaa atcaacccca acctctgcac gcagtgcgtc ggacactacg
    20341 atgagccgca gtgccagcag gtttgcccgg tggactgcat cctgattgac gaagaatatc
    20401 ccgaaaccca tgacgagttg atggcgaaat acgaaaagat tatccagttt aaataaattc
    20461 tttttaaaac atcaaattat gtctgttttg aaataaaatc aaaaaaaaac ttgacggaaa
    20521 agcaagccgc taataaacta acgttctctt ttggaggggt tcccgagcgg tcaaaggggg
    20581 cagactgtaa atctgttgcg aaagcttcga aggttcgaat ccttctccct ccaccaaaat
    20641 tcttacttgg ggcagtagcg agtaatgcgg gtgtagctca atggtagagc agaagccttc
    20701 caagcttacg gtgagggttc gattcccttc acccgctcca acaattaggc ccatgtagct
    20761 caggggtaga gcactccctt ggtaagggag aggccggcag ttcaaatctg cccatgggca
    20821 ccatctctcg attattcatt tctttaaggc ttagatatat aggatattg
//
LOCUS       DAWKOG010000021        19821 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.021, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000021 DAWKOG010000000
VERSION     DAWKOG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19821
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.021"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(232..307)
                     /locus_tag="WJK66_000583"
     tRNA            complement(232..307)
                     /locus_tag="WJK66_000583"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(272..274),aa:Asn,seq:gtt)
     gene            complement(400..759)
                     /locus_tag="WJK66_000584"
     CDS             complement(400..759)
                     /locus_tag="WJK66_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEZ7578332.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            complement(782..1426)
                     /locus_tag="WJK66_000585"
     CDS             complement(782..1426)
                     /locus_tag="WJK66_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEZ7578333.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADA
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            complement(1428..1964)
                     /locus_tag="WJK66_000586"
     CDS             complement(1428..1964)
                     /locus_tag="WJK66_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEZ7578334.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            complement(1976..3073)
                     /locus_tag="WJK66_000587"
     CDS             complement(1976..3073)
                     /locus_tag="WJK66_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ7578335.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLASVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            complement(3076..4512)
                     /locus_tag="WJK66_000588"
     CDS             complement(3076..4512)
                     /locus_tag="WJK66_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEZ7578336.1"
                     /translation="MENSLIIGLALAVLLMLLVMRAKQGKKTPKRKSQGVGNTQGQTP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGPATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQHADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            complement(4732..5514)
                     /gene="bioC"
                     /locus_tag="WJK66_000589"
     CDS             complement(4732..5514)
                     /gene="bioC"
                     /locus_tag="WJK66_000589"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEZ7578337.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKANGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQTWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFSMPSGKVHLTYHPLFFIARYSAAGRQ"
     gene            complement(5502..6149)
                     /locus_tag="WJK66_000590"
     CDS             complement(5502..6149)
                     /locus_tag="WJK66_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HEZ7578338.1"
                     /translation="MKTKFYNHQGEHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRLKFERRMCGDKVSFEDYQQFPARPFDEIHQELTALFEMIGQDRR
                     ADLIRWTNALVGSGDKIFMPANQHRYWTPRCTVREIDGGHYLFSRFTHWSALWNH"
     gene            complement(6159..7301)
                     /gene="bioF"
                     /locus_tag="WJK66_000591"
     CDS             complement(6159..7301)
                     /gene="bioF"
                     /locus_tag="WJK66_000591"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEZ7578339.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENRKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEATLAK
                     AEYLQRQGYYCLPIRPPTVPKGTSRIRLSLTADMTMDEVRQFAACL"
     gene            7524..8600
                     /locus_tag="WJK66_000592"
     CDS             7524..8600
                     /locus_tag="WJK66_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEZ7578340.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCVRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            complement(8807..10273)
                     /locus_tag="WJK66_000593"
     CDS             complement(8807..10273)
                     /locus_tag="WJK66_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEZ7578341.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFFAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(10471..11394)
                     /gene="speB"
                     /locus_tag="WJK66_000594"
     CDS             complement(10471..11394)
                     /gene="speB"
                     /locus_tag="WJK66_000594"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEZ7578342.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLTAPQVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(11493..13385)
                     /gene="speA"
                     /locus_tag="WJK66_000595"
     CDS             complement(11493..13385)
                     /gene="speA"
                     /locus_tag="WJK66_000595"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEZ7578343.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVCPNPSQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(13552..13836)
                     /locus_tag="WJK66_000596"
     CDS             complement(13552..13836)
                     /locus_tag="WJK66_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ7578344.1"
                     /translation="MKKLLLAAVVSLSAATAFADDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVHDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(13987..15795)
                     /gene="aspS"
                     /locus_tag="WJK66_000597"
     CDS             complement(13987..15795)
                     /gene="aspS"
                     /locus_tag="WJK66_000597"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEZ7578345.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSARNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALNVDLGDFPRMPYAEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIARTGAE
                     NGDIIFFGADKTKVVNEAIGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKVAETKEA"
     gene            complement(15853..16554)
                     /locus_tag="WJK66_000598"
     CDS             complement(15853..16554)
                     /locus_tag="WJK66_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEZ7578346.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPK
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLHVKTL
                     AGPMPSEKAELPEQQ"
     gene            complement(16697..17845)
                     /locus_tag="WJK66_000599"
     CDS             complement(16697..17845)
                     /locus_tag="WJK66_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEZ7578347.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPTVSAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            complement(18006..18269)
                     /gene="rpsT"
                     /locus_tag="WJK66_000600"
     CDS             complement(18006..18269)
                     /gene="rpsT"
                     /locus_tag="WJK66_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEZ7578348.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            18572..19714
                     /locus_tag="WJK66_000601"
     CDS             18572..19714
                     /locus_tag="WJK66_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7578349.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LNPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
BASE COUNT         5114 a         5115 c         4584 g         5008 t
ORIGIN      
        1 agaatcgttc tctttgagct aaggcgagcc aatgccgtac tggtttttgt taatccacta
       61 taaaatcgag tttatcctag ttgtccagga cagcccctat tttttcataa ccatcacgaa
      121 aggaattttg caaagatttt attccatctc aaaacaatca tctcaaaaat gcgtttctga
      181 ccgccggtaa aacaaaaccc tctaagaaaa tacttagagg gttttgaaat ttggctcccc
      241 gacctgggct cgaaccaggg acctgcggat taacagtccg tcgctctacc gactgagcta
      301 tcggggaata agcgcgaata ataaaacaga aataccgaac gtgtcaatat aaattgacaa
      361 tcgattgcac tttatctgca agcccgcaat ctttatatgt tatatggctt ccgcaatatg
      421 ccttgccgca cgtttggcat ccaataggat taaaccaagt ctgccggttt cttttgccac
      481 cagcaccaac acggcatctt tacccgcctg acttaaaagg atatagcctg attttccttt
      541 aatcatcact tgttccaatt ccccgcaggc gagttcctgc accgagcgac tccccaaagc
      601 aagcaaagtg gcagaaatcg cccctaccct gtccgaattc aaatgtgaag gaagcattgt
      661 cgccatcggc aatccgtcgg ttgagataac ggcagacgcg ataatatccg tagatgtatt
      721 gtttaaatct tcaaggattg aaatcaataa ttgctgcata acccctcctc tctcccaagt
      781 tttacacgcg gttgctgtaa cggcggtata aaatccttac caaagtaaca aatgcctctt
      841 tgcccaaatc gggaatgccg ccgataacca aaataaattt ggttgaaccg atatacaatg
      901 ggaaaaatgt caattcgctc tgaccggaag gatcgcaaac gccccaagcg ttattgttga
      961 tatacaggtt gttcttaatc agcagccggt atttcttttc catctgtgcg acttctgccg
     1021 ccaacaaccc caactcttcc gccgcctcat gatggaaatt ggcgttggca agatacagac
     1081 cgttccgatc gaccaataac gccttaccgc tgccggacaa ttgttccatc agcaacggca
     1141 attgctcgtc cgacaaattg atgccgtctg aatgaccgtt ttcatcgcca tagaggaatt
     1201 cgagtttttg caaacggtac aacaggttca aagcggtatc gatgtcggcg gtgtccgccc
     1261 aggtaagcag cttctcactg ctgaccattt cgtccgcatc cgctttcaac aggctgtgca
     1321 acaaagtttt accggcactg ggggcatcgc tggatacggc ataaaatgca ccggcaggag
     1381 tcaggcgggg atataaattt gcttgtagtg aaagtgttga ttccatatta aacctccagt
     1441 cccggatcaa tagaaaataa cattgcgcta accaattgtt ttacgtcatc ttccttacgg
     1501 gcatcaattt caaaaaccgg aacattaaga ttatgttttg caagatattt gtgatacacg
     1561 tcgataccgg gctgagagcg tatatccatc ttggtaatac cgacaacgac gggtgccttc
     1621 tccagcagcc ctcgaaacga atgtaaaaag aattccaaat ctttcaacgg attggttcgg
     1681 gcattatcta aaagcaagac caaacccata ctgccttggc ttaagatttc ccacataaag
     1741 ttgaaccgtt cctgaccggg cgtaccatat aaatggactt tggtatcctc atccaagctg
     1801 atggccccgt agtccatcgc cactgtcgta ttccttttcc tatccaaagt catatcggat
     1861 gcggaagcat cggtctgaac gagtgcttcg tccgaaatag ccgcaatggc agtggttttc
     1921 cctacgccga caggtcctgt gaaaataatt ttattttctc tcatctctcc gcctcttagc
     1981 tgcccaacag ttttttcatc agcctttgca gaaggccgcg cgactgggat tgggatggtg
     2041 ttgtgatttt ttccgctttt ttcatcattt cactatcaga ggcagaatcg gctccgatat
     2101 ttattttatc cgccatatcg gagtatgcct gttgtgaaac cgtttttaaa tctaccgaca
     2161 aaaacccggt tgtataggtt gccgcaagat aattcagaat atcattgagg tttaaaggca
     2221 tcactttata caacacgtta aggttgacgg atgccttggt cagaaatgcc gacaagcgta
     2281 tcgaccccgg cacacttgcc aaccgggtca ggtttggcca agatttcaac gtaaacggag
     2341 tatcgggaga aatcggataa atcaacctgc cctgcgccgt ccaaatggaa aactgccaca
     2401 tacaggacat aatgcctact ttagcctttt cgcgccattg cgggttatcg ggaacagtct
     2461 tgcagctgac ctgcaaattt tcgtctttgc acaattcttc gagtttttgc acactttctg
     2521 tcagcaaaac ccgttgtatc gaggggaaaa caataaggac cggcttattt ccatgcaaga
     2581 tagcgatgtc ctgcctgttc ttttccgcaa accgcaacgc ccccaataat cctttattcg
     2641 ggttaaactg gcgtatcgta accgtacgct gcacattccc gttatttttc gccgaccggt
     2701 ctgcgggtgc aataaacgat ttcccataaa cattctcgcc ctgcaacaat ttgcggagca
     2761 taggaaacaa tgtttcaaac cgaatcggtt tgggcaggta gggaacttca gaatcgggaa
     2821 ctttctccga acagacggcg acgggtatat ccttataacg ctcggcaagc tctttccaaa
     2881 gttcaaaacc gccctcggca tcggtatccg ccaaaaccaa atcgggcacg gcactgccgt
     2941 ctgaagggga tactgtttca taacgggtgg tattgtgcat tttgaatgcc attttgaaaa
     3001 cggattcctg ctgcgccgtc atccccgcca acattacgcg tactgtttta attttcggca
     3061 gttgaacttc catttttatt ttccgtaccg ttttattttt ttaatatttt gattcatacg
     3121 ctgcagcagc cggctcatca gcataacgac ctcttcagga agcctgtccg cacgttccct
     3181 caataccctt aaaaactgcc ccaacctatc ccaatcttca gtacgttcat aaatatcgat
     3241 caacgtaata taaagctggg actcgtcggg atatttcaat accgcctgct ccaacacatc
     3301 cattgccgct tcaatctgac catacatcag caacgactct acttctttaa ccgcatcgtc
     3361 tgccggagac gaaccggtgt tgatcaacga agaatcttga agcaccaaat cccgatgttg
     3421 cgatttgaat ttctgtatat ttttcggcag atacccgtgc cccataccga tgtctttgat
     3481 ttgccggtcg ttcggccctt tttccaaatc atcgaaaact tcatggtaac ccaagctgta
     3541 cccccacccc agcatccgct ctttaacctg cctgccgtag ttgcccaacg tttggtaaag
     3601 tttccacaaa tgtccggcaa aacggtctat gtccgcgtgt tggtaatcga gtttcaacgc
     3661 atcgataatc aggtttgcag gtttttcgga agtttggatg gcacggtcgt attgtttcga
     3721 tgccgtttca tagctgactt tgtctttaag gattttcgca ccttgatcgg cacggaccaa
     3781 accggcaatc gcaccgattt cctcttgact gattccggac acgtcttttt tgccccgcac
     3841 aatcgggatg cgcttgattt cttcggcttc ataagcctta ccgccggcat ccggcggggg
     3901 cgatgcggaa gctgccgtcg caggaccgcc ttccgcacgt ttttcaatct cctgagtacc
     3961 ccatcccaaa ccttcttccg ccaagacgcg gatacgcaaa tggtttgaat cgcgctgtaa
     4021 tgcctgttcg atatattttg ccaaaagttc ggaaggaatc agtttgccgt atttttgcag
     4081 attgtctgcc aaaacatcga catcccccac ttcgagattg atatcgagca gctcgcggat
     4141 aaggttttca ggtttcgctt caccatccgg aatgccgtcc agataggcag ccaaagattc
     4201 ggcagccttg ccctgataac cgaattgctt ataaacctga tactccgtaa gcggatcgac
     4261 ttcttgcgcg gatacggcgg cggatgcggt ctcggcactt tcgttccaag accagtcggg
     4321 ttgcgtgccg tctgaaaccg attgtccaat ctgatcgacc cagtccgaat cattgcttcc
     4381 gggtgtctgc ccctgcgtat tgccgacacc ttgggatttg cgcttcggtg ttttcttacc
     4441 ctgcttcgca cgcataacca aaagcatcag caataccgcc agtgccaaac cgataattaa
     4501 tgagttttcc aaaggatact cccgataccg taacgagcgg atagctgacc gcctgcaacg
     4561 gccattgtcc gcttttatta ttaacgattt actgaaatag acttgtaagt tttaaatcat
     4621 accataattt aacgtttaac aatatgcctt ctgcacaagc ctcgccatat taccttttac
     4681 ccacatcagt atcaataccc gatataaaaa taactttgcc cataagctgc cttattgcct
     4741 gcccgccgca gagtagcgcg cgataaaaaa taacggatga taagtcaggt gcactttgcc
     4801 cgacggcata ctgaacgccg acaaataatc gccaataaat ccattgagat tttttcttgt
     4861 ccaatttttt tggttcgtgg ctgttacgcc tgtatattta aggtgtttga gtacatctaa
     4921 cggcgtatca aagtctagta ttaccttaaa atcctcacac cataaaagct caaaatcttt
     4981 ggctaaccaa gtctgccatt gggataaagt cgggtaattt aagcctatat ttgtaatttg
     5041 gcggacttct tttaaattgt ctttgccaaa ggttgcaacc gccaataatc cgtttgcttt
     5101 caagcctgtt ttgcaatggg cgataaaagc gtcgggttga tgaaaccatt gcacggcaga
     5161 tgcgcttgcg attaagtcaa attgtcgttg aaaaggaaag ttttccgcat cgccgcaata
     5221 aaaatcaaag gattgcggca gtttttcagc cagttggggc tgcacatcgc acaaatcatt
     5281 aaataaccaa taattcgctg aaatctgttt ttgcagcaag gcactcaaca ttcctgagcc
     5341 gcagcccaat tccaacacgt tttccaatgg catatccggc aaataatctt gcaaatgcgc
     5401 cattaaatta atcgtcattt tttgttggat taaggcgtgc cggtcataat cgtttaatgc
     5461 tttttggaaa gcctgccgaa tgcgcgattt atttatggca gtcagtgatt ccatagtgcc
     5521 gaccaatggg tgaatcttga aaacaggtaa tgtccgccgt caatttcccg aacggtgcaa
     5581 cgcggtgtcc aatatcggtg ctgattggca ggcataaaaa ttttatcgcc cgatccgacc
     5641 aaggcatttg tccagcgaat caaatctgcg cgcctatctt gcccgatcat ctcaaaaagt
     5701 gcggtaagtt cttgatgaat ttcgtcaaac gggcgtgcgg gaaattgttg gtaatcttca
     5761 aaagatactt tatcgccaca cattctgcgt tcaaatttta aacgggtgtt ttccgtgagg
     5821 ttctccaatg tgcctttaaa aacggcgcaa gggataccga aattatcatc gcaaggcaaa
     5881 cctgtgccat tcactgccgt tgcggatttt aatcttattc cttgcaatgc cctctctgcc
     5941 gcccaaacgc ccattgacca cgccaccaaa cggatgtgcc gataggcgga aaaatcaaaa
     6001 tccaaattta aatcttgata atcatagcaa atcaataaat cgtgattttc cggcaaaatc
     6061 aaatgattta cagcatcggg cggcgttccc caacctgcaa aatacaggat taaatgttcg
     6121 ccttgatgat tgtaaaattt tgttttcata tcatatcctt acaggcacgc cgcaaactgc
     6181 cgcacttcat ccattgtcat atctgccgtc aaagacaatc gtattctgga tgtacctttt
     6241 ggtactgtcg gtggtctgat gggcaggcaa taataaccct gcctttgcag gtattccgct
     6301 ttggcaaggg tggcttcatt cccgcctaaa atatagggga cgatacaggt ttggctcggc
     6361 attatttgcg tccgatgcgc cacttcccgc cgtaaaaatg cgcttaactg ctcaagatgg
     6421 cttctttctt ttgagaattg cggcaatcgt tcaaaaataa aataagtcca agccacatta
     6481 aacggcggca atgcggttga aaaaatcaat gggcgcattt gattaatcaa acattctttc
     6541 aatacttggt tgcagacggc atacgccccc accgaggcta aggctttacc gaaagtgccg
     6601 accaataaat caatctcggc aatcaaatta tcccgttcgg caatccccaa tccgttttgc
     6661 ccataaacac cgattgcgtg agcttcatcc acataaagat aagtattggg aaactgtttt
     6721 tttaattgga caagctgttt caaatccgcc acatcgccgt ccatactgaa aacagattcg
     6781 gtaacgataa aagtgcggtc aaattttccg acgttttttt caagcagatt tttcaaatgt
     6841 tcataatcat tatgacgata acggaaaaac gcacaccggc tcaaacggat gccgtcaatc
     6901 atactggcgt gaacaaattt atctgccaaa atcaaacttt tcgtcgtcgt caaagcaggc
     6961 aaaataccga gattggcgtg atagccgctg ttgaacaata acgcgctttc ccgttggaaa
     7021 cgttgtgcga caagctcttc caaatcggta taaataggaa agttgcccgt taataaacgc
     7081 gatgaagaac tggtaaaaga gggaaaatta ccgccgtatt gctgcaaaaa agaccggcgc
     7141 aagttttcat ctgatgccaa acccaaataa tcattagacg acatattcag cattttgcgg
     7201 ttttcccgcg taatataccg cccttgatga atcaaatccg gaatcgaacg atattggttt
     7261 tgcgcgccga gttgttcaag ctgttgttta aaaaccttca ttatgatgta aatattcctg
     7321 agttaaagcc tgaaccaacc cagcatcaaa caatcaagta atgccctttt aataaaaacc
     7381 gttttgccgc cgacctttgc cacacccctg ccttcataat ccaaggcgga aatttccgcg
     7441 acatttgttt ccgttgccat tacccaatcc gtccgcactg taccgaagcg catattttcg
     7501 cacaaattgg tacggtttca ggtatgattt gccgaaaacc tatcggacac acaccatatt
     7561 ttgatatgaa taaaacaatc tgccgcactg ctgcactatt gataagcggt ttttcatacg
     7621 caaacaccgt cattcccgat gtctcccccg tcgcacaagg tcaacatgtc ttcattaaca
     7681 tcccccagca acgcctgttc ctctacaccg acggcaaact gaccaaggtt tatcccgttg
     7741 cagtaggtcg ggcgatgacg caaaccaatc taggcgaaca taaaatcggt gcgaaagcct
     7801 ataaccctgt ttggtacatc cccaaatcca tacagaaaga acgcggcgac ggcgtaaaaa
     7861 ccatagccgc cggcccggac aaccctttgg ggccggtttt tgtccgctta ggcgacccta
     7921 aactcggttt gggcatacac gggaccaatg cgccggccag cgtccccggg gttcggagtc
     7981 acggctgcgt ccgcatgaaa tcgcccgacg cgctcgagtt tgccaaaacc atcgccagcg
     8041 gctcgcccgc ctccgtcatc tatcaaatgg cgggtctcaa tgaagatgcg gatcgcaacc
     8101 tgtggcttgc cgccttccgc gacccttacg gtaagaacaa ccttgacatc gcctctctga
     8161 aaaaaagtat tgcgcaatgg gcaaaaacac agggtaaaac catcgcgccc gagaaagtcg
     8221 atgctgtact caaagaccgc accggatcgg ccgtctgcct gacctgcggc aaaaacggca
     8281 agatgaagat gccgctcaaa tcgctggcgt ggatacaggg ttcttcctca tacagccaag
     8341 ctgaagtcat tgaacaaact gaggaaacca actctgccga aatatccgag acacgcacgc
     8401 ccgaagtgcc tgatgtacac acgcccgagg cacaaccgca tttaaatacc caatccgacg
     8461 gcacgccgac tgcctatacc gaaccggctg ccgattcatc gccgcaagta gaaacacctg
     8521 atcaggctgc ttccgagccg gttgatgtat tattttcaat agatgtgata cggcagggaa
     8581 atttgcgttt aggtaattga ataaccttct gattatttaa tgcttatgtt tatcagaagt
     8641 tgataggcgg tttggttttt gtttggcttt tcctacaccg ccgcctgccg ctttctgcaa
     8701 cattcaagcg cacaaatatg ccatctgaag gctttagacg gcatatttca cgataatagt
     8761 cgcccaatca aataaacaca gattggaaat ttattttatt tttcccttac caatacccca
     8821 gaactttcca ccaaatgctg ccgataacga aaaaaatcag aaaattgact acgctcatga
     8881 taaaacccgc cttccaccat tctcccattg tggtgtagcc cgaaccgaaa atcacaggcg
     8941 aagtaccggt cgcataatga gtgagggtca tcataatgtt agatgcggcc gccatcatca
     9001 gcgcggtcgg catcgccggg gcattcagtg aaacggcagc agcgaaaaat gcgccgaaca
     9061 tagcggtaat atgtgcagta gtactggcaa acatataatg cgcatacata taagcaagca
     9121 cgaggattac gcccgcagcc gtgccgctaa cgcccaaacc gccgacactt tccgccaaca
     9181 ctccggagaa ccatttaatc agtccgagtt tatttaaaaa tgcggccatc ataatcaatg
     9241 cgccaaacca aataatcgta tcccacgcac ttttttcttt caaaacatcg tcccaagtca
     9301 atacaccgga aagcaaaagc aggcttaatc cgataaatgc ggtggcggtg gcgttgatac
     9361 taaaagcgtg attgccggta ataagggcgg gaacatctgc ccacaacagc agcaagatac
     9421 cgaaaatgac cgccataatg atttcgtctg ccgacatttt acccatctcc ctcagacggt
     9481 ctttggcaaa ttgaacggca ttgggcgttt ctttaatttc aggcggatac aaaaaatata
     9541 aaatcaaagg cataacgaaa aaggcgataa cgccgggaac agccattgcc cacgcccacg
     9601 ccccccaaga aagacggaaa ctactgccta aattttcggc aatcaagttg acgattaaag
     9661 ggttgggggc agttgcagta ataaacatag ccgacgaaat gggattggaa tgatagttga
     9721 ccaaagccaa atatttaccc atcttgcctt ctgtgccttt tgcgggattg gagccgtaac
     9781 tgccggcaat cgactgcata atcggatgta taatgccgcc gccgcgcgcg gtattggaag
     9841 gggtaacggg agccagcagc agttcggaaa gagcgagact gtaaccgatg cccagcgttt
     9901 ttcttccaaa aacggcgata aacaaatatc cgatacgcat ccccagccct gttttgagca
     9961 aaccgcgcga aatcataact gcgatggcaa tcagccaaat caacggattg gcgaacgcac
    10021 tcaacgcatc gctcatcgcc gcgcccggtt tgtcggcggt tacgccggtt actgcgacca
    10081 acccgacggc aataatcgac agcgcgccca acggcatggc cttgccgata atggcggcaa
    10141 tcacaccgac aaacatggcc agcagcgtcc aagcctgagg cttgaccccg tcgggtacgg
    10201 gcagtgccaa aaccagggcg cacaatactg cggcaatggc gaggggtatc ggtttgaaac
    10261 ccaatttcat catattgacc tccgtaaaaa agaccgtccc gaaaaatcgg aaaaataata
    10321 tttaactaat tgttttataa gatatattca gatatttcac cgtctttccg atatgcggct
    10381 ccgggcaact tttgtttcag tatttgaatt ttcattagac tgaatacgcc gtttgaacgg
    10441 cacggcgaaa acccggggga tgccggacgt tcagtccttt ttcgcacctt gaaggtaaag
    10501 catttccaag gcaattgtgg cgccggccaa agcggtaatg tcggattggt cgtaagaggg
    10561 ggcaacttct acaacatcca taccgacgat gtcgagatcc gtcagcccac gtaggatttt
    10621 taatgccctg tcgctgctca agccgccgca tacgggcgta ccggtcccgg gggcgaacga
    10681 cgggtcgagg cagtctatgt cgaaagtcag gtaaacgggc atattgccga cggtttcttt
    10741 gattttacgg acggtctctt caacactgtc ttcattaact tggggggcgg tcaacacagt
    10801 aaaaggcaat tttttactgt gttcggtacg tatgccgatt tgtacggaac gggacgggtc
    10861 gatgaggcct tccttggggg cggtatagaa catcgtaccg tggtcgtatt cgctgccgtt
    10921 gtcgtaggtg tcggtgtgcg cgtcaaaatg aatcagtgcg agtttgccga aatagcgggc
    10981 gtgggcgcgc aacaacggga gggtaatgaa atggtcgccg cccaaactca aacagcgttt
    11041 gccggaagaa agtaatttgc cggcgtgcgc ttccattttt tcgacaaaat ccctgctgtc
    11101 gccaaaagaa aaaaccaagt cgccgcaatc aataatgttc aggcgttcgc gcacatcaaa
    11161 tgtccatgga aacctgcggt gctcccaagc gaggttgacg gaggcgcgcc ggatggcttc
    11221 aggaccgaaa cgcgcgccgg aacgccctga aaccgccata tcataaggca cgccggtaat
    11281 aacccaatcg gcatgacttt catacggcat aaaattaagc ggcaggcgca aaaacccgaa
    11341 attattggaa acgagggagt tgtcggtttg tcctgccagt gtgctgtatt gcatcgtaat
    11401 gattccttgt aattggtttc aatcggtcgt gatgattggt gtttgagtaa gaaaatcggg
    11461 cttcagacga catatccgat gccttgatgc gtctattcgt cttccaaata ggtataacca
    11521 ttaagccccg cttcgagttc ttttaagaaa gacatagcct gcgaggcagg aaggtctgaa
    11581 tgttcgattt gttcgcgata gcgtttcatc agctctttcg gatcttgata aacgtattcg
    11641 agcatatcgg caacggtgtt tccttcatcg taatcgatga cggtaaattg tccgtcttcc
    11701 cctacaacaa catcggcagt ggcagtgtcg ccgaaaagat tgtgcatatt gccgagtatt
    11761 tcctgatatg ctcccaccat aaaaaagcct aaaagcggcg gctcttcttc gggataatca
    11821 ggcataggca tcgtaccggc gatgccgtct ccgtcgatgt agtggtcaat cgtaccgtct
    11881 gaatcgcagg taatgtccaa caacacggcg cggcgcgcaa tcggttcatt caaaccggta
    11941 atgggacaaa cagggaaaag ttgatctatg ccccaagcat cgggcaaaga ttggaagagt
    12001 gagaaattga catacagctt atcggcaaaa cgttcttgca attcgtcaat aatggttcgg
    12061 tgagaccggt gtttttcatt aaacaattcg ccgacttcat gacagatatt taaatacagt
    12121 tgctccgccc acgcacgttg cgccaaactc aacagcccga cattatactg attatgcaca
    12181 tcggcaagat caaactgccc ttcgtgtatc cagctgcgta aggaacgttt ttcccgcgag
    12241 gcggaaatat ccgtccaagt ttcccacata ctgtgcaaca cacgcggcgc ttcgggcgat
    12301 ggcgcatcca gccggcgcgg tttgtaacgc tcaacgccta taacattagc aaccaaaacg
    12361 gcgtgatgtg cggtaatgcc gcgcccgctc tcggtgatga ttgtcggatg cggcagcccg
    12421 tgttcgagac aagcctgact gatgccccat acgactgtgg cggcatattc gttgaggctg
    12481 taattaacgg aacaatccga ttgtgtgcgg tttccttcgt aatccacgcc aagcccgccg
    12541 cctacatcaa aacagcggat atttaccccc agtttgtgca actcaacata aaaccgagcc
    12601 gattcgtgta cacctgtggc aacatcacgg atgttcccaa gctgcgagcc caaatggaaa
    12661 tgcaaaagct gcaggcaatc cagcctgttt ttttgtttca aaatatcgac cagttgcaaa
    12721 acttgggaag ccgacaagcc gaattttgat ttttccccac ccgaagactg ccattttccc
    12781 gaaccttggg aagccagtct ggcgcgcaca cccaaacggg gcttgatgcc gagtttttcc
    12841 gcctcttcca ataccatttg tatttcggac agcttctcaa tcaccaaata aacctgatgc
    12901 cccagttttt cgcccatcaa ggcgaaacgg atatattcac ggtctttata gccgttgcag
    12961 acgattaatg tttgccgggt gccggcgtgt gccaaaaccg ccatcagttc ggctttagaa
    13021 ccagcttcca aaccatgcgg ttgtccgctt gacataagcg attcgatgac gcggcggtgt
    13081 tggttgacct tgatagggta aaccaaacaa taaccgccct tatagccgca ctcttcccgt
    13141 gccgtctgaa aggcgcggtt aatgtcgcgg aggcggtgtt cgaggatttg cggaaaacaa
    13201 aacaaaacag gcaggcgcgc ctgatgtttt tgttgcacgg cttcagtcag tttttgcagt
    13261 gaaacagttt gattgtgttg cgagggattg gggcagacga tgatttcgcc ggaatcgtca
    13321 acatcataat aacctatgcc ccaatgatta atgttgcaca cttcacggat ggtaaggata
    13381 ggcataataa acctgctccg tctgtcgtgt tgaaaaggaa tgattataac aaatcagcgt
    13441 gaaatgtcat ttttttaata agaaaagcct gcctcatacc tgatgaggaa caggcaaaat
    13501 gtcgtctgaa cgcttcagac ggcattttgg ttcatctttc catcaaagga gtcagcgatc
    13561 gagctgctct ttgatgattt tcaggtcggg gtaagacaac acgatgtcgt attcgcggcc
    13621 gtctttatag gcttccactt ccaaaacagg tttgccccaa tggtcgtcgg catcgacatc
    13681 gtgaacctga taaccgcgct gttccaacat ttttacggct tttgtgcggt tttgttcaaa
    13741 atggggatca ccgtaaatct gacgctcggc agaatcgtcg gcaaatgcgg ttgcggcact
    13801 cagggaaaca acggcagcca ataacagttt tttcattttc agtcctttct taatcggttg
    13861 attgaataag atgtgttttt caatgctgcc attaaaacac agcaaaatta ggtttgaatt
    13921 agcaggaagt taagaagcgt aaatgccgtc tgaaaaaaac agagccgtca aacggctccg
    13981 ttttccttat gcttctttgg tttcagccac cttttgacgc aggcgcaagc tcaattcgcg
    14041 caactgcttg tcgtccacgc tgttgggcgc gttggtcagc aggcattggg cacgttgtgt
    14101 tttcgggaag gcaatcacgt cgcggataga ttcggcgccg gtcatcaggg taaccaaacg
    14161 gtcgaggccg aatgcaaggc cgccgtgagg aggcgcaccg aatttcaggt tgtccaagag
    14221 gaagccgaat ttctcttgtt gctcttcagg gctgattttc agcgcggcaa acactttctc
    14281 ttgtacgtct gcacggtgga tacggataga gccgccgccg atttcccagc cgttcaatac
    14341 catatcgtag gcgcgcgcca aacaatttgc cgggtcggaa accatcaggt cttcatgacc
    14401 ttctttaggc gcggtaaacg gatggtgtac ggcaacgtag cggtcggctt cttcgtcgta
    14461 ttcgaacatt gggaaatcga caacccacaa aggtttccat tcgtctgtga agtagccgtt
    14521 gtctttgccg tgttccaagc cgactttgat acgcaatgcg ccgatggctt cgttcacaac
    14581 tttggttttg tccgcaccga agaagatgat gtcgccgttt tccgcgccgg tacgcgcaat
    14641 gatttctttc agggcattct cagacaggaa ttttacgatt ggagattgca ggccgctgtc
    14701 ttcgccgttt gagaggttgc ccacatcgtt tactttgatg tatgccagac ctttcgcgcc
    14761 gtagatgccg acaaatttag tgtattcgtc gatttctttg cggctgaatt ttgcgccgtt
    14821 cggtacgcgc agggcgacta cacggccgcc tttcatatct gccgcgccac ggaagacttt
    14881 gaattcttcc gttttcatca ggtcggtcaa ctcggtaaat ttcaagttga tacgcatatc
    14941 cggtttgtca gagccgtagt agaacatggc ttcggcgtaa ggcatacgcg ggaagtcgcc
    15001 taaatctacg tttaaagcat ctttgaagac ttgtttcgcc ataccttcgg tgatgtccat
    15061 aatttcatcc tcgttcaaga acgaggtttc caagtcgatt tgagtgaatt caggctgacg
    15121 gtcggcacgc aagtcttcgt cacggaaaca tttggtaatt tgatagtaac ggtcgaaacc
    15181 ggccaccatc aacagttgct taaataattg cggtgattgc ggcagcgcga aaaactcgcc
    15241 cggatgaacg cggctcggca cgaggtagtc gcgcgcgcct tccggagtgg agcgggtcag
    15301 catcggcgtt tcgatgtcga tgaagccttg cgcgtccaga taacggcgaa cgcccatagc
    15361 gacttgataa cgcaggcgca ggttgcgttg catcaccgga cggcgcaagt cgataacgcg
    15421 gttggtcagg cgaacgtttt cgctgatgtt ttcatcgtcg atttggaacg gcggcgtggc
    15481 ggcggcgttc aagacttcga tttctttggc aaggatttcg attttgccgg aaatcatttt
    15541 atcgttggtc gtgccttcgg gacggttgcg tacgcggccg gtaatgctca aaacgtattc
    15601 gttgcgagcg gaatcggcag cggcaaacgc ttcgggcgtg tcgggatcga tcacgacttg
    15661 gacgatgcct tcgcggtcgc gcaggtcgat aaaaatcaca ccgccgtggt cgcgtcggcg
    15721 gtgtacccag cctttgacgg taacggtttg gtctaagtat tgctcgctga tcaggccgca
    15781 atagttggta cgcataaaat caccttttat tgatttaaac tgaaaacaga aaatgccgtc
    15841 tgaacggcgg ctttattgtt gttcgggcaa ctccgccttt tcagacggca taggtcctgc
    15901 caatgttttg acgtgtaggt cgtcagggat gaccataccc agcgaaatca catatttcaa
    15961 tgcttcgtcc acgctcatat cgagttcgcg cacatcgctt ttctttacca taatatagta
    16021 accgccggtc ggattcggcg tggtcggaac atacacggaa agataatcgc cgtccttcgg
    16081 caatgcggcc ttaaccgcat tcgacacctg accggacacg aatgcgattg tccaaatacc
    16141 cgattgggga aacggcacga gtactggtgt tttaaacgaa cggctgctgt cggacagcaa
    16201 cgattcggat acttttttca cactcgaata gatggacttc acaaccggaa tccgccccaa
    16261 caagctgtcc cacgcggcaa gaatctgccg gcccaatacg tttgcggcaa ataatccggt
    16321 tacaaacaat acagcaatgg caacgataac gcccagcccc gggatattaa accccaaaac
    16381 atattgcggc cgccattgct tcggcagcag gttgacgagc tgatcggacg cggaaacgat
    16441 ataggaaacc acccaaaccg ttaccgcaat cggcagccag accaaaatgc ccgtaatcag
    16501 atattttttt aacgccttgg cagctttacc gccttcggcc gcaggttccg tcatcttgct
    16561 tgattccgac aaagtccgta caaaccgcac attatacgcg tttgcccgga ttcaaacgaa
    16621 atttttatcc cgccccgcca aaccgccggc ggcttcagac ggcacggcaa cttgatatac
    16681 cgtctgaaca cgcggttcag atgccgtcca agtcgttaaa catcaacccg ataccgatac
    16741 cgttctgctt gtggttgtag tcgatcaggc tctcgccgta accgtggaat ccgcgtacca
    16801 cgcctttgag tttgccctta atcggaaacg tgtaggcggc ttcaatcgcg ccgtagcccg
    16861 ttttggggtt gtagcgcaat acggaataca cattctgcct gtcgttcagg cggtactgca
    16921 gcttcacgtc gccatacccc atatagtcgg caatatcggg attgtcgttt ttatcgccgc
    16981 tctgatcgaa cgcacgcacc cacacgcgcg gaatcaccgt caatttgccc cattccatgc
    17041 ctgccatggc gtaaatcctg ttccacgaac gcgattcggg acggctctgt ccgttggact
    17101 ggtggacaaa acccgcaccg agcatacgca gcctgccgcc gaacggcaaa tccgccttca
    17161 caggctgggt caggaaaatt tcaggtttgt aatccgtatt gcggaacggc gcggatttcc
    17221 tgccttggtt gtaaatctgc caatcggatc tttgggtgta gccgaaccac agatccgcgc
    17281 gggttttaaa caaatcttcg gcaattttgc ttttgaacga aacctgcaat ttggtttccg
    17341 cacgtttctg ctgtccgaat ttttcctgta cagtcgtacc gcgcgtcggc gaacccgggg
    17401 catagttggg cgaattgttg taccagagcg gcataaggta catcggattg tgttcgcgta
    17461 cgcccaacag cccgcgcaaa tcgtttttgt ccaagtcgta catcaggctc aaaggcgtat
    17521 agatgtcggc ggtttcgccc gcactgtcgg caggaagcgc atccccgcct ttttcaacaa
    17581 caatgaccgc ctcgccctta tccaggctgc tgcggacggt ttccgtcaga ttgagtacgg
    17641 ctttcgactc ctgcccttcc tgccctgccg aagacggaag ctgtgccgca aaaatcctgt
    17701 cgtaacacgc caaacgcgta acattgtccg tcaaagcggc gcattgcagc gcggtctctc
    17761 caaaagcgga taccgtcggc aacagtcctg tcaaaagaat atagcgcata ttccgtgtat
    17821 tcatctccgc ccccattgtc ggcatattgg ttttcaaacg gcattttata gcggattcgg
    17881 ataaaaaacc gcaccctttc cgccatttcg ggatttagcc cccgtaatac agaaaacccc
    17941 gaaaccgtcg ggcttcaggg ttttccgctt atcgcgtatc aaccgccttg gcggttttgc
    18001 aaaaatcaag ccagtgcttt tacttttgca gacaaacggc ttttgtggcg tgccgctttg
    18061 tttttgtgga acacgccctt gtcggcgatg cggtcgatga ctttgacgga ctcttggtaa
    18121 accgcttgtg cggcagcttt atcgcctgct tcgactgctt tcaatacttt tttcactgca
    18181 gtgcggaatg cggtacgcag gctagcgttg tgggcgcgtt gtttgacgga ctggcgggca
    18241 cgtttgcgtg cttgtgcgct gtttgccata ttggaatctc ctggaagaaa aataaattct
    18301 gtaaacgcgc aattttaaag ggggtttaca ttatatgcaa gccttttctc tctatccgcc
    18361 cgtcggacac cctgacagcg caaaaaaacg gcaaaatcgg gcaaccgttt tcctcccctt
    18421 ttaaaaacag gataattttt taggaaaatc agtccaatat cgctttgagt ttgcacaaat
    18481 ttactttaca atgcccaaac cttgcgccgg ggtgtttatg gggcgcgggg ttttcttttt
    18541 ttccaacctc tttcatcagg aatctgcccg tatgaaaaaa tccgtattag ccgttttggc
    18601 cgcgctgtct ctggcagcct gcggcggcag cgaaaaaaac gccgtgcaac ctcaagcaga
    18661 cgctgcgtct gctgccaatg ccgaagccgc tgccaccgat actctgaaca tctacaactg
    18721 gtcgaactat gttgacgaga gtacggtcga agactttaaa aaagccaaca atctgaagct
    18781 cacttacgac ttgtacgaaa acaatgaaac gctggaagcc aaaatgctga ccggcaaatc
    18841 cggttacgat ttggttgtgc cgggcatcgc cttcctaccg cgccaaatcg aggcgggcgc
    18901 gtatcaaaaa gtcaacaaag acctgattcc caactataaa aacatcgatc ccgaactctt
    18961 gaaaatgctg gaaaccgccg acccgggcaa ccagtatgcc gtcccctatt tctccggcgt
    19021 gaacacgatt gcgattacgg cgaaaggcaa agagctttta ggcggcaagc tgcccgaaaa
    19081 cggctgggat ttgctgttca aacctgaata cacccacaaa ctgaaatcct gcggcatcgc
    19141 cctgtgggac accccgagcg aaatgttccc gattttgctg aactacttgg gcaaagaccc
    19201 caaaggctcg aatcctgaag atttgaaggc ggcggcggaa gtgttgaagt ctatccgtcc
    19261 tgatgtcaaa cgtttcagcc cgtccatcat cgacgagctg gcgcgcggcg acatctgcct
    19321 ggcggcaggc aacggcggcg atttgaactt ggcgaaagca cgttccgaag aagtgaaaaa
    19381 caacgtcggc atcgaagtgc tgaatccgaa aggcatgggc ttctggattg agtcttggct
    19441 gattcccgcc gatgcgaaaa acgtcgccaa tgcccacaaa tacatcaact acacgctcga
    19501 ccccgaaatc gcggcgaaaa acggcatcgc cgtaaccttc gcccccgcca gcaaaccggc
    19561 gcgcgaaaaa atgcctgccg aactggtgaa cacccgttcc atcttcccga acgagcagga
    19621 tatgaaagac ggtttcgtga tgccgcaaat gagtacggat gcgaaaaaac tgtctgtcag
    19681 cctgtggcag aaaatcaaag tcggcaccaa ctgatttgaa gcattaaaaa tgccgtccga
    19741 acatcgttcg gacggcattt tatattggat tgaaatagaa atatttatat agtggattaa
    19801 caaaaatcag gacaaggcga c
//
LOCUS       DAWKOG010000022        18431 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.022, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000022 DAWKOG010000000
VERSION     DAWKOG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18431
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.022"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..163
                     /locus_tag="WJK66_000602"
     CDS             <1..163
                     /locus_tag="WJK66_000602"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578350.1"
                     /translation="APIAKAMMLNQGFCEAFIVVVLMGIAAIMRKRPSFSKPPHRFRV
                     CPSSRPDLQ"
     gene            200..412
                     /locus_tag="WJK66_000603"
     CDS             200..412
                     /locus_tag="WJK66_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEZ7578351.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILEGVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
     gene            560..2143
                     /locus_tag="WJK66_000604"
     CDS             560..2143
                     /locus_tag="WJK66_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HEZ7578352.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESKAFSQTAI
                     NALEARARMMGLQPAPAANAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTVDESFGKFDYIIVHGIWSWVPDAVKDKIFSICRNNLT
                     EHGIAYISYNVYPGWKRQEQLREIMYFAGRDLLEEPLEARTRKGLDALKALAEILEND
                     KGLGGGGKLPAIQKILNHNTYYVAHEYMEIFNDPIYVNGFIEWANRHRLAYIGDTDLH
                     VSFVSWMAEHTRERILALAGDDYIAKEFYSDILSDRQFRRSLLCREEVGDNIRRDESV
                     AVEVIESLNFRPARGETINFDENDTLLSGIRDVMKTGEPFKTEDVAENLARRFPGLEF
                     DRMKINSQLLLQTILGRFSVSSDNAGKPFFEDHKTYVPARFTNYAAAFVEHGAEAFVR
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQALIETVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSAD"
     gene            2227..3324
                     /locus_tag="WJK66_000605"
     CDS             2227..3324
                     /locus_tag="WJK66_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEZ7578353.1"
                     /translation="MNHTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEGLFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKAGGQVLFGFNATDGAFEVVPQVFLWQLSFTDNIAVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQESIPSYAEALKREGHNPFFSA"
     gene            3438..4553
                     /locus_tag="WJK66_000606"
     CDS             3438..4553
                     /locus_tag="WJK66_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEZ7578354.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNTLP
                     ACGQALLNLASVSTENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIVGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            4678..5133
                     /locus_tag="WJK66_000607"
     CDS             4678..5133
                     /locus_tag="WJK66_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEZ7578355.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKIRQSDAAAFDCIIAMDGKNLSELERTFGR
                     RSEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            5204..5680
                     /locus_tag="WJK66_000608"
     CDS             5204..5680
                     /locus_tag="WJK66_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEZ7578356.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            5668..6594
                     /locus_tag="WJK66_000609"
     CDS             5668..6594
                     /locus_tag="WJK66_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEZ7578357.1"
                     /translation="MSEMKKNKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVNPRQFINPDYAQQALYKDQIGICDVLVASKTD
                     LCTTEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIRWLDIPVIEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            6659..7216
                     /locus_tag="WJK66_000610"
     CDS             6659..7216
                     /locus_tag="WJK66_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ7578358.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDNTIFHRVIGGFVIQGGGLTEDLAQKASDKAVANESGNGLKN
                     TVGTIAMARTADPDSATSQFFINLADNDSLNYKNGQYGYTVFGRVESGMNTVSKIARV
                     KTATRGFYQNVPVQPVKIRRVVVGQ"
     gene            7590..9281
                     /locus_tag="WJK66_000611"
     CDS             7590..9281
                     /locus_tag="WJK66_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ7578359.1"
                     /translation="MNWLGKSYARLLRNLPPETLISEDKTHNAKPENAGSQNLLIRGD
                     NLEVLKHLKNAYTNSVKMIYIDPPYNTGSDGFVYQDDRKFTPAELACLANIDEDEAAR
                     ILDFTDKGSNSHSAWLTFMYPRLYIARELLKDDGVIFISIDDNEAAQLKLLCDEVFGE
                     GNFIKDLIVNTSEGGGNAKYVVNGHETVLVYAKDITHFDNLKRPKDIRGKKIVINDEL
                     YWIQEDAVREQFGKYGNLHYEDIIEIKGIEFKNKIDKDIENNEYILIPKSYGKTIIGK
                     LRKVSDDFSKFHSILNIGNISKNLTKDGIRELEELFETGKGYSPFETPKPLDLLKRLV
                     LSATFKGNQTDLILDFFAGSGTTAHAVMQLNAEGQNGNRRYICVQLPEKTAEKSEARK
                     AGYPTIFDITKARIEKAAAKIRVEHPDYTGDSGFKIFQTADNFRQHPDKDFSPEQPDL
                     PLNDELSEEQLQTLLTTWTLYDGAALTTPVEPVRLGAYTAYLCEKRLYLMNAGFTSAD
                     LLAFIRKLDDDADFNPNRVIVFGSNMASAMQHELDQAVRGYANKKEIELNVVIRV"
     gene            9294..12233
                     /locus_tag="WJK66_000612"
     CDS             9294..12233
                     /locus_tag="WJK66_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HEZ7578360.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRAADENPELLFAA
                     KQYSDNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTRQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSPSMTDTGDKALKDLFDNPVDALAAVRPFV
                     IVDEPHKFPTRESAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSDGKEAKFELNEKDKKQTFKLAKGEDLAKIHPAVSDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIHAEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLVLVNQLFSDGIIDDNENFTK
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFVDLFTAYLRENAAKFPQAGIRTAVNEAYINNGLMLSRRIDSIEDEDFIR
                     FNTMTYREFLEKLAQTAKIRMQTLHQAFYRIRNELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYRLVGSKIHPTKFTNKDGKPRAVKKADLGRFEDTEHRPAAGYLFDEIF
                     YDSDIEHENVANNQIEGVTVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTKSGEILNFV
                     IEAKGTDGAEDLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAG
                     GHSENGH"
     gene            complement(12327..13286)
                     /gene="ttcA"
                     /locus_tag="WJK66_000613"
     CDS             complement(12327..13286)
                     /gene="ttcA"
                     /locus_tag="WJK66_000613"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEZ7578361.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIEPGDKIMVCLSG
                     GKDSYALLDILRQLQASAPIDFQLVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATLFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFAGLERGQTLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIAPQKAERKVINILANKPKTCGA"
     gene            complement(13384..14247)
                     /locus_tag="WJK66_000614"
     CDS             complement(13384..14247)
                     /locus_tag="WJK66_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HEZ7578362.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINNLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATIKRLRMFVLRAKVVFEILEDYAVDAEL
                     EASAEPLAAQEPSLVFTAECVSDGICTVVLPHRGILHIAPKNALPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAVEAGTLLTADGEETGIVLDSVQDSENFTALAVIKFSAAQKELTAPNGSIFKA
                     VHPFFKTENAE"
     gene            complement(14282..14644)
                     /locus_tag="WJK66_000615"
     CDS             complement(14282..14644)
                     /locus_tag="WJK66_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ7578363.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWCVEIKITAVKKDAATA"
     gene            14719..15198
                     /gene="ybaK"
                     /locus_tag="WJK66_000616"
     CDS             14719..15198
                     /gene="ybaK"
                     /locus_tag="WJK66_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEZ7578364.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKHIEPATPVQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPRDLNILNPKTIQAAV"
     gene            15221..15943
                     /locus_tag="WJK66_000617"
     CDS             15221..15943
                     /locus_tag="WJK66_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEZ7578365.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPKAEE
                     KFKEIQRAYDTLSDLSKRMQYDASFRRHEERGRQEEAFRREQARREQFYREQMRREQA
                     FRQAFERQASRSCHTYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVIFAYMPIVG
                     VILAYMKRNSLDSIVYAAHTEYLIKTFWRTFWLYILGALTALLGIGVLIIIATNVWYF
                     YRIIAGFIRFNGGRAVAPEKWI"
     gene            15945..16820
                     /locus_tag="WJK66_000618"
     CDS             15945..16820
                     /locus_tag="WJK66_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ7578366.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDVGNIGAFLPTWWLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGRLIGLCLAFAALFCLLWKHSGGKKSGSAWRQAVLLLGVWAGYGI
                     IDILFKQLAKSGTAFAGNLLVAFVLAGVLMFACLFAKSVRWRVESVVGGIFLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWGEVKVLFGI"
     gene            complement(16892..18289)
                     /gene="aspA"
                     /locus_tag="WJK66_000619"
     CDS             complement(16892..18289)
                     /gene="aspA"
                     /locus_tag="WJK66_000619"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEZ7578367.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGELGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLVVLKNAFAAKAEEFKDVLKMGRTQLQDAVPMTTGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAINLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVSKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
BASE COUNT         4769 a         4427 c         4874 g         4361 t
ORIGIN      
        1 tgcgccgatt gccaaggcga tgatgctcaa ccagggtttt tgcgaagcgt tcattgttgt
       61 cgtcctgatg ggaattgccg ccattatgcg caagcgtccg tctttttcca aaccgccgca
      121 ccgtttccgc gtttgcccaa gttcacgccc cgacttacaa taacgcccgt cagattgcag
      181 ttccaacgaa aggaaaatga tggaaaccct tatcctcgac atcggcggca tgagctgcgg
      241 cggctgcgtc aaaagcgtta cccggatatt ggaaggcgta aaaggcgtgg caagcgtcga
      301 agtcagtctt gaaaacaaaa gtgcgaccgt cggatacgac cccgcgcaaa ccgatgcagg
      361 ggcgttgatt gaagccgttg aagacggcgg ctatgatgcg gcgttgaaat aaagcggcaa
      421 aaatgccgtc tgaagccttc gcgcctcccc gatgccgtct gaagtacgct ccgcgccttc
      481 agacggcatt tgatttagtt gaacgggtag gatgaattgg ttaaacttgc gggcaatagt
      541 gccttcagac aaacaaaata tgccgaacaa aaagaaaaaa gccgcctttg cgcgtgctgc
      601 aacggagaac ggtgtgccgg acgtgtccaa catcaaaaat tcatatgatg atttgatgta
      661 tgaatccaaa gcattttccc aaaccgccat caacgccttg gaggctcgcg cccgcatgat
      721 gggtttgcag cccgcgcccg ccgccaatgc caaggtcttg gagctgggct gttcgatggg
      781 cggcaacatc atcacgcagg ctctttatta cccggatgcg gaatttgtcg gtatcgactt
      841 gtccggcagg caggttgcgc agggcaacgc catcattgaa aaaatgggct tgaaaaatgt
      901 gcgtctggaa gaaaaagata ttttgaccgt cgatgagtca ttcgggaagt ttgactatat
      961 catcgtccac ggcatttggt cgtgggtgcc tgacgcagtt aaagacaaaa ttttttcgat
     1021 ttgccggaac aacctgaccg aacacggcat tgcctatatt tcatacaatg tttaccccgg
     1081 ctggaaacgg caggagcagt tgcgcgaaat tatgtacttt gccggcaggg atctgcttga
     1141 agaacccttg gaagcgcgga cgcggaaagg cttggacgcg ctcaaggcgc tggcggaaat
     1201 tttggaaaac gacaaaggct tgggcggcgg cggcaaactt ccggcgattc aaaaaatatt
     1261 gaatcacaat acttattatg tcgcacacga atatatggaa atattcaacg acccgattta
     1321 cgttaacggc ttcatcgaat gggcaaaccg ccaccggctg gcgtatatcg gggataccga
     1381 tttgcacgtg tcttttgttt cttggatggc ggagcatacg cgggagcgga ttttggcatt
     1441 ggcaggggat gattatattg ccaaagaatt ttatagcgat attttatccg accgccaatt
     1501 ccgccgttcg cttttatgcc gtgaggaagt cggggacaat atcaggcgtg atgagtcggt
     1561 tgccgtcgaa gtgatagaaa gtttgaattt ccgcccggca agaggggaaa cgatcaactt
     1621 tgatgaaaac gataccctgc tttcgggcat acgcgatgtg atgaaaaccg gagagccgtt
     1681 taaaacggaa gatgttgcgg aaaatcttgc ccgccgattt cccggtctag aatttgaccg
     1741 catgaaaatc aattcccagc ttttattgca aaccattctc gggcgttttt ctgtttcatc
     1801 ggacaatgcg ggcaaaccat tttttgaaga ccataaaacc tatgtgccgg cgcgttttac
     1861 aaactatgcc gccgcctttg tggaacacgg cgcggaagcg tttgtccggc ctgccaaccg
     1921 ttacaacgaa agcacgccct cattcgggta cgggcatttg tacattatgc ggcaattatc
     1981 gcggccgacg agcaaacagg cattgattga aacggttgcc gaaaacttga acatcgtcag
     2041 cactacgccc gacggtttga cattccatcc gcctgccgaa gtgtatgtgg aagaaatatt
     2101 ggcagacttg gcagacaggc attttctcgt ttcggcggat tgacgggcaa gtcccgacgg
     2161 caatccgtcc gatatatttt cagacaggaa agacacacac actgcgaaaa gcggtacacg
     2221 acacatatga accatacatt taccttgccc gatacgcgcc cgtatcccca aaatccgatt
     2281 aaaaaccacc tgctgctcaa tgcctaccag ttggcgcata attcttccca ggcttcgcgc
     2341 aaactctcgt ccggccaact tcaaaccgaa atcaggggga tgcttgagca aaaccactat
     2401 atcaaccttt ccctcgcgct gacgatgtcg cccgatgccg gaacttatgc cgcgctgctt
     2461 tccagtgtga acgcggtact cgattgcgag aaagaaggcg aagtgcagtg gttcgccctg
     2521 ccggtcgtgc tggtgtccgg ctgcaagaaa gaacgggcaa tcgagatgaa gctgccgacg
     2581 gagggattgt ttgcctgttt gcaaaactat ccgcacctgc gcgcgttgac gcaagagacg
     2641 caatggctgc cttatcttgt acattcctcc gatttgagtg cggtcgcgcc ggatgagtgg
     2701 tggcgtgcca aacaaaatac cgaagcggcg gcgcaacact tacgccgttt cgccccgcgc
     2761 cctttgcttt tgcccgaagg gcagtccgtc cacgtcgttt acgcgctggg tttcggcagc
     2821 ggcaaagttc agacggcatt gggtcagaac cttcttcagg caggtctgcc cttgatgcag
     2881 gtatggcagg aaaatcttgc atcggaaggc gttacgctgt ttgccaatcc gctttccccg
     2941 gattctccgg tacgcgcgct ttccgacggc agccacacgc gccagcgtat ggcgatggat
     3001 gtgtttgcgg caaacgcgat acgcgccgtc cgtatgcaga gtccgcgcgt cggcgtggtc
     3061 gctgcggcaa aggcgggcgg acaggtttta ttcgggttta atgcgaccga cggcgcgttt
     3121 gaagtcgtgc cgcaggtgtt tttgtggcag ctttccttta ccgacaacat cgccgtcatc
     3181 cagcaaaatt tcctcgacct gatggcggag tgccgcgtgg aacacgttta cctgttgcac
     3241 aatcccttgg gcgaacagga aagcatcccg agctatgcgg aagcattgaa acgggaaggg
     3301 cacaatccgt ttttcagcgc atagtgattt gtagtagtga taggctttct catttatata
     3361 ataaatcaaa ataaagagca caacactttt tcattctgtg ttgtgccttg agtgaaacga
     3421 aaggatgaat tataaatatg aagattgtaa ttgcaccgga ttcgtttaag gaaagcttga
     3481 cagctcaaca ggtagctgaa gcaataaaaa gaggcttcca acaatcgata gcagatgtgg
     3541 aatgtctcct ctgtcctgtc ggtgatgggg gagaaggcac tgtagatgct atccgacatt
     3601 ctcttgacct agaagaaaaa tgtcttcaag tgacaggacc ttttggacaa aaagaagtca
     3661 tgcgctattt tcaaaaagaa caactagccc tattcgaagt tgctgatttg gttggtcttg
     3721 gaaaaatccc gctagagaaa cgaaatccat tacaaatcca aactcgtggt attggagaat
     3781 tgattcgcca cctcattagt caagaaatta aagaaatcta tattggcgtt ggcggtacgg
     3841 ccagtaatga tggaggtatt gggattgctg ctggtttagg ttatcaattt tatgatgagg
     3901 atggaaatac cttgcccgct tgcggtcaag ccttactaaa cctagcttct gtttcaacag
     3961 aaaatcgcta tgaaattcct gaagatgttc acattcgtat tttagcagat gttgtgagtc
     4021 ccttatgtgg acaccaaggt gcgacctata cgtttggcaa gcaaaaaggg ttggattcta
     4081 ctatgtttga ggccgtagat caggcaatac aagattttta tgaaaaagtc tcccctgcaa
     4141 cattaaaact taaaggagca ggagctggtg gaggcatcgc tggtggtttg tgcgcctttg
     4201 ctcaggcaag tatcgtatct ggaattgaca cctgcctgga cttgattgac tttgataaga
     4261 aagtgtcaga tgttgacttg gttatcgttg gtgaaggaag actagatcgt caaagtttag
     4321 cagggaaagc gcctattggt gtagcaaaaa gaacccctgt cggagttcct gttgtcgcta
     4381 tttgtggcag tctcgttgaa gatttgcctt ccctgccatt tgaaaatatc caagctgcct
     4441 tttctatttt ggagaaaagt gaacctttag aagatagttt gaaaaatgcc agtctctatt
     4501 tggagcatac ggcttctaat attggacact tattaaatat gcctaagatt tagccaaacc
     4561 atttcttcca gatggatgtt ttggctggtt ctgccttatg cggttacgat taaccgaccg
     4621 atgccgtctg aaagggtttc cgcttcagac ggcatcgcgc cggaacaggg aggggatatg
     4681 aaaaaaccga aaatcctttt tgtctgcctc ggcaacatct gccgttcgcc gatggcggaa
     4741 tacattttgc gccgccgcgc cgccgaagcg ggcattcccc ttgaagcgga tagcgcgggg
     4801 acatcgggct ggcacgacgg cgaagatatg caccgcgaga cggcaaagat attgaaaaaa
     4861 cacggtatcg atgcttcagg ctttaccagc cgcaaaatcc gccaaagcga tgcggcggcg
     4921 tttgactgca tcatcgcgat ggacggcaag aatttgtccg aattggaaag aaccttcggc
     4981 aggcggtcgg aaaaaatatt caagctgacc gacctgatac ccgaaagcgg ttacgaccat
     5041 gtccccgatc cgtggtatac gggcgatttt gaagaaacct tcaggcttgc ggatgcgggc
     5101 tgtcgggcat tgttggaaaa gatttccaaa taaagcaatt gaatacaaat ataaaaccgc
     5161 ctcctgccgt atcggttgtt cagacggcat aaacagagaa tttatgaaaa caaatttcaa
     5221 acagaaaatt atcgaacagg cacgcagcga aggcttgcag gtaaccgctt tgcgcgagca
     5281 ggtattagat attgtcttgc agcaaagcgg cgtgattaaa gcctacaacg tcttgtcgca
     5341 gatgcagcag caaagcgagg gcgtgcttgc accgcctacc gcctaccgcg cccttgattt
     5401 ttgggcggag cagggcgttt tgcacaaagt ggcggcggtc aacggctata ttttgtgcag
     5461 ccacgcgcag cacgagtgcg acgaccattg ccacgaccac gaagaagccg aagcgcacca
     5521 cagcgcgttt attttggtct gcaccgaatg tggcacggcg gacgagcaaa ccctgagcca
     5581 cgagtgggcg gcactgcgcg caggcgttgc cgaaagcggc tttgcgctga aagaagaaca
     5641 cgttgtttta accggaatct gtaaaaaatg tcagaaatga aaaaaaacaa agtccacctg
     5701 atttcaggtt ttctgggaac aggcaaaacc accgcgctca aaagcctgat ggcgcaaaaa
     5761 gacccgaacg aaaaatgggt catcatcgtc aacgagttcg gcgagatcgg tattgacggc
     5821 gcggtattga gcgacaacgg catccctgtg gcagaaatcg ccggcggctg tttgtgttgc
     5881 accgccggcc cgcaaatggg cgtaaccgtg cagaaaatgc tgcgcgacgc caagcccgac
     5941 cgcctgatga ttgaagcaag cggactggct cacgcagcca gcgtcatcga cgaactcaaa
     6001 gccaaaccgc tggacagcct tttggaaatc ggcgccgtct ttaccgtcgt caatccgcgc
     6061 cagttcatca accccgatta cgcgcaacag gcgttgtata aagaccaaat cggcatctgc
     6121 gacgtattgg tcgccagcaa aaccgattta tgcaccaccg aacagcttgc cgaatttcac
     6181 gacaaagcgg caaaactgtt cccgcccaaa gccaaagtgg tcgaagtcca aaacgcacaa
     6241 ctcgacatcc ggtggctcga tattcccgtc atcgaaaaat cacgctaccg cctcaaagcc
     6301 ctgccggaca acaccatggg cttccagtcg caaggtttca cattccccgc cggacgcgat
     6361 ttcgacggcg aaaagctgac caactttttc aacgacctgc ccaaaatgac cgaaggactc
     6421 gtccgcgcca aaggcgtgtt tcaagtattg ggaacgtggg tgtggctcaa ctgggtagac
     6481 ggacagtggg gcgcgaacca agtctcatgg cggcgcgatt cgcgttttga attgattgcc
     6541 aaatcgtttg atgcggattt aatcgaacaa aagctcaaag acgcattgga atagcgttca
     6601 cgcgccttca gacggcatat gaaatgaaaa tgccgtccga aggtctgagg aggacgggat
     6661 gaaacccaaa ttcaaaaccg ttttaaccgc gctgcttttg gcggtttccc tgccgtctat
     6721 ggcggcaacc catgttttga tggaaaccga tatgggcaat atccgtttgg ttttggacga
     6781 atccaaagca cccaaaaccg ttgccaattt cgtgcgctat gcccgaaaag gcttttacga
     6841 caatacgatt tttcaccgcg tcatcggcgg cttcgttatc caaggcggcg gattgaccga
     6901 ggacttggca caaaaggcaa gcgataaggc cgttgccaac gaatccggca acggcttgaa
     6961 aaacactgtc ggcaccatcg ccatggcgcg gacggccgat ccggattccg ccaccagcca
     7021 attctttatc aatctggcgg acaatgattc actcaactac aaaaacggac aatacggcta
     7081 taccgttttc ggcagggtcg aaagcggcat gaacaccgtt tccaaaatcg cccgcgtcaa
     7141 aaccgccacg cgcggctttt atcaaaacgt acccgtacag cccgtcaaaa tccgtcgcgt
     7201 tgttgtcggg cagtaacacg cagacagatg ttcagacggc gtcgcccgtt tcacaaaaaa
     7261 cgccgtctga agtgaaaaaa tattttaaaa cagatagttg atattgacaa attcaaaccg
     7321 aggattttaa aatgctgcca acccaaccca acccaaccca acccaaccca acccaaccca
     7381 acccaaccca agcccctgat ctaatcgaca cagccggggt gaacaccgag gcaatctaca
     7441 cttcagacgg cattaccgcc aactctaccc aactcgaaca gctcaaaaaa ctgtttcccg
     7501 cctgttttga cgcagacgga aatttcctta tcgacagatt acaagccgaa attgcgccac
     7561 agaccgacat cggacgcgaa ttttacgaaa tgaactggct gggcaaatca tatgctcgcc
     7621 tgcttcgcaa cctgccgccc gaaaccctga taagtgaaga caagacgcac aacgccaaac
     7681 ccgaaaatgc aggcagccaa aacctactga ttcgtggcga caatctagaa gtgttgaaac
     7741 acttaaaaaa cgcctacaca aacagcgtga agatgattta catcgacccg ccctacaaca
     7801 ccggatcaga cggctttgtc tatcaggacg accgcaaatt cacaccagcc gaacttgcct
     7861 gcttagccaa tattgacgaa gacgaagccg cgcggatttt agatttcacc gacaaaggct
     7921 caaactcgca cagtgcatgg ttgaccttta tgtatccgcg cctgtatatc gcccgcgaac
     7981 tgttaaagga cgacggcgtg atttttatct cgattgacga taacgaagcg gcgcagttga
     8041 agttgttgtg tgatgaagtg tttggggaag ggaattttat taaagattta attgttaata
     8101 catcagaagg tggtggtaat gcaaaatatg ttgtaaatgg gcatgaaact gttttagttt
     8161 atgcgaaaga tattactcat tttgacaatc taaaaagacc aaaagatata cgcggcaaaa
     8221 aaattgtaat caatgatgaa ttatattgga tacaggaaga tgccgttcgc gaacagtttg
     8281 gcaaatatgg gaacttgcat tacgaagata ttattgaaat aaaaggaatt gagtttaaaa
     8341 acaaaattga taaagatatt gaaaataatg aatatatctt aattccgaag tcttatggga
     8401 aaacaattat tggaaaactt agaaaagtat ccgatgattt ttccaaattt catagcatct
     8461 taaatattgg aaatataagt aaaaatctta ctaaagatgg tattcgtgaa ttagaagagt
     8521 tgtttgaaac tggaaaaggt tattctcctt ttgaaacacc aaaaccatta gatttattga
     8581 aaagattagt tctttctgca actttcaagg gaaatcaaac tgacctaatc ctagacttct
     8641 tcgcaggcag cggcacaacc gcccacgccg tgatgcagct taacgccgaa ggacaaaacg
     8701 gtaaccgccg ctatatctgt gtacagcttc ccgaaaaaac cgctgaaaaa tccgaagccc
     8761 gtaaagcagg ctacccgacc atcttcgaca tcaccaaagc ccgcatagaa aaagccgccg
     8821 ccaaaatccg cgtcgaacat cccgattaca cgggcgattc gggcttcaaa atctttcaaa
     8881 cggcagacaa tttcaggcag catccggaca aggatttttc gcccgaacaa ccggatttgc
     8941 cgcttaacga tgaattaagc gaagaacagc tgcaaacgct tctgaccacc tggacgctgt
     9001 atgacggggc ggcactgacc acgccggttg agcctgtgcg gttaggggct tacacggcgt
     9061 atctgtgcga aaaacggctg tatctgatga atgccggttt tacttccgcc gatttgttgg
     9121 cgtttatccg caagctggac gacgatgcgg atttcaatcc caaccgcgtg attgtattcg
     9181 gcagcaatat ggcaagcgcc atgcagcacg aacttgacca ggcggttcgc ggttatgcca
     9241 ataaaaaaga gattgagttg aatgtggtta tcagggtttg acggaggcaa ttcatgagcg
     9301 gttttaatta cgagaaaaac cagccgcacc aaatgcgggc ggtttcggcg gttttgggcg
     9361 tgtttgacgg ggcaacgccc aaatatcggg cggcagacga aaatcccgaa cttttgtttg
     9421 ctgcaaaaca atactcagac aatatcctga aagtgcaaag ccaaaacggc atagacggcc
     9481 gattccccga ccgttcggac gaccaaaata tccttgatat ttccatggaa acgggcacgg
     9541 gcaaaaccta tacctacaca caaaccatgt tcgagctgca ccgttggctg ggcgtgttca
     9601 aatttatcgt ggtcgtgccg actttgtcca ttaaggcggg aacgcggcag tttttgcaaa
     9661 gcaaggcttt ggcagagcat tttgaacagg atttcggcgg cgattatgaa ggcgtacgcc
     9721 tgaaaaccta tgtggtggaa agcgcgaaaa agaataaggg caaaaagtcc aatgcgccca
     9781 taacgatcga gcaatttgtc aaagcggaaa acaaaaagga aatccatgtg ctgctgatta
     9841 acgcgggcat ggttaattcg ccgtccatga ccgatacggg cgacaaggca ttgaaggatt
     9901 tgtttgacaa tcccgttgat gcattggctg ccgtgcgccc gtttgtgatt gtggacgaac
     9961 cgcataaatt cccgacccgg gagagcgcga aaacgtgggg caatatcaaa cgcttaaaac
    10021 cgcaatatat tttgcgctac ggtgcgacat ttaacgatga atattacaac ctgctttacc
    10081 gtctgacggc tgtagacgcg tttaacgacg ggctggtcaa aggcgtgcgc gtgtttcagg
    10141 aagaaatgca gggcggcatg gatgcggcgg taaaactggt gtcgtcggac ggcaaagaag
    10201 cgaaatttga attaaacgaa aaggacaaaa agcagacgtt caaactggca aaaggcgaag
    10261 atttggcgaa aatccatccc gctgtttcgg atttgaaaat cgacaaaatg aataaaaccg
    10321 tggtggtgtt aagtaacggc ttggagttga aaacgggtgc cgtcatcaac ccttattcct
    10381 attcgcaaac ggtgcaggat gcgatgatgc agcgggcggt tgccgagcat ttcaagctgg
    10441 aacgtgcgct tttggcagaa cgcgcgccac agcccaaaat caagccgctg acactgtttt
    10501 ttattgacga tattgcgggc taccgcagcg gcaacgagct ttcaggcagc ctgaaagata
    10561 aatttgaaag ctggattcac gcggaagcgg cacgccgcct gaaaacggaa agcgacccgt
    10621 tttaccgcga ttacctgcaa aagacgttgg acgatgtatc cgcctgccac ggcggttatt
    10681 tttccaaaga caatacagac agcgacgata gaatcgagca ggaaatcaat gaaatcctgc
    10741 acgataagga aaaactgttg tctttggaca acccgcgccg ctttattttt tccaaatgga
    10801 cgctgcgcga aggctgggac aatcccaacg ttttccagat ttgcaaactg cgttccagcg
    10861 gcagcacgac ttccaagctg caagaagtcg gacgcggcct gcgcctgccg gtaaacgagc
    10921 tgatggcgcg ggtgcgcgat gtgccgtaca aactgaatta ttttgtcgat agcagcgaaa
    10981 aagactttgt gaagcagctt gtcggcgaaa tcaacgataa ttcttttcag gaagaaatct
    11041 ccaaaaagtt taccgaagag ctgaaacaaa aaatattgca aaaatacccc gatatcaaac
    11101 cgctggtatt ggtaaaccaa ctgttttcag acggcatcat tgacgacaat gaaaacttta
    11161 ccaaagacgg ctatgacaaa ttaaaagccg cctatcccga agccttcccc aaaggtttgg
    11221 acaaaggcaa agtcagcaac gccaaagacg agggcaaaga caccatcatc atgcgcgaag
    11281 gcaaatatga agaactcaag gccttgtggg agctgattca ccataaagcc gttttgcagt
    11341 acaaaatcaa ggatgaagcc gaatttgtcg atttatttac cgcctatttg cgtgaaaacg
    11401 ccgccaaatt cccgcaggca ggcatacgca cggcggtaaa cgaagcttat atcaacaacg
    11461 ggcttatgct ttcccgccgc atagacagta ttgaagatga agattttatc cgtttcaaca
    11521 caatgactta ccgagagttt ctggaaaaac tggcacaaac ggcaaaaatc cgtatgcaga
    11581 ctttgcatca ggcgttttac cgcatccgca acgaactgaa cattggcgat tttttgaata
    11641 tgcaaaccat cgcccaaatc aaaaacggct tcaaccggtt tttgcttcat cattccttcc
    11701 ataaattcga actggattac cggcttgtcg gcagcaaaat ccatccgacc aaatttacca
    11761 ataaagacgg caaaccgcgc gcggtgaaaa aagcagattt gggcagattt gaagatacgg
    11821 agcaccggcc tgccgccggc tatctcttcg acgagatttt ctacgattcg gatatagaac
    11881 atgaaaatgt cgccaacaac caaattgaag gcgtaaccgt atttaccaaa ataccgagaa
    11941 actccatcaa aatccctgtt gccggcggcg gcacgtattc gcccgatttt gcctatatcg
    12001 tgaaaaccaa aagcggcgag atcctgaact ttgtgattga agccaaaggg actgacgggg
    12061 cggaagattt gcgaaaaagc gaagagcgga aaatcaaaca tgccgaaaag ctgtttgccg
    12121 agatttccaa agaaatcaag gtggtgttca aaacgcagtt tgacggcgag aggatagccg
    12181 aactgatcgg gcaaaatatg cccgcaggcg ggcattctga aaacggacac tgagaaacaa
    12241 atgtttggaa gccgtctgct tcgggatttt aaaatgccgt ctgaaacgcc aaacgggctt
    12301 cagacggcat ttttgacggc ggggggttac gcgccgcagg ttttcggctt gttcgccaga
    12361 atgttgatga ctttgcgttc ggctttttgc ggcgcgattt tgatttcgct ttcgtcttct
    12421 tcgctgccgt ctgaaaaacg ttcgggcatt ttttcgctgt caaacgccaa atcgccgccg
    12481 tgtttcaggg tttgaccgcg ttccaatccg gcaaagtcga agagttcggt atcggcaagg
    12541 tgggaaggga cgacgttttg cagggcggag aacatcgatt cgatgcggcc gggaaagcgt
    12601 ttgtcccaat cgcgcagcat atcgccgatg acttggcgtt gcaggttggg ttgcgagccg
    12661 cagaggttgc acgggatgat ggggaattgt tttaattcgg cgtatttgat taagtctttt
    12721 tcttttacat acgccagcgg acggatgacg atgtgttcgc cgttgtcgct caccagcttg
    12781 ggcggcatgg ctttgagttt gccgccgtaa aacatattta aaaacaaggt ggcgaggatg
    12841 tcgtcgcggt ggtgtcccaa ggcgattttg gtgcagccca attcttttgc agtgcggtag
    12901 aggatgccgc ggcgcaggcg gctgcacagc gaacaagtcg ttttgccttc gtctaatacg
    12961 cgtttgacgg tggagtaggt gtcttcttca acaattttgt agggaacgcc gatgctttcg
    13021 agataggtcg gtaatacttc ttcggggaag cccggctgct tttggtcgag attgacggca
    13081 accagttgga aatcaatcgg cgcgctggct tggagctggc gcaggatgtc caagagggca
    13141 tagctgtctt ttccgccgga gaggcagacc atgattttgt cgcccggctc gatcatgttg
    13201 aaatcgttaa tcgcgtcgcc gacggcgtgg cgcaggcgtt tgctgagttt gttgttttcc
    13261 agttcttgtt tggttttttt ggacatggcg gtggtttgaa aaattgggga tgcggcattg
    13321 taaccgaaaa caggggcggt tttaaaatgc cgtctgaaaa aggttcagac ggcattagag
    13381 ggattattct gcattttcgg ttttaaagaa gggatgaacc gctttgaaga tactgccgtt
    13441 tggggcggtc agttcttttt gcgcggcgga gaatttaatc acggcaaggg cggtaaagtt
    13501 ttccgaatct tgaacgctgt cgagtacgat gccggtctct tcgccgtctg ccgtcagcag
    13561 ggttcctgct tcgacggccg aatttcccga caataccgcc aagccgcgtt tgacctgacc
    13621 gcgatactgg gcacgggcaa tgatttcctg tcccggatag cagccttttt tgaagtgtac
    13681 gccgccgatg atgtgctggt tgagcatttg ggcgacggcg gtttctttgg tagccgcgca
    13741 tatccacggg taaccgctgc ggatttcgtg cagccgccac gcgttttcgg cggcggcatc
    13801 ataaggcggc aaggcgtttt tgggggcgat gtgcaaaatg ccccgatggg gcaggacgac
    13861 ggtgcagatg ccgtctgaaa cgcattcggc ggtaaagacg agactgggtt cttgcgcggc
    13921 aagcggttcg gcggatgctt ccaattccgc gtcgacggcg taatcttcaa ggatttcaaa
    13981 aacgacttta gcgcgcagga caaacatccg caggcgtttg atcgttgctt caagcaagtc
    14041 ttgcgccata atcagcagca aatcgccgcc tcggttgacg acaatcatat tggcgatgac
    14101 gcggcctttg ggcgtgttgt aagtcgcata acacgcctgc ccggtctgaa gattattgat
    14161 gtcgttggaa agctgtccgt gcaggaaggt ttggcggtct tcgccgctga cgcgtaccac
    14221 gccgaaaaag ggcagtaagg ttttcatcat ttgcgtactc tgaaatataa aggaaatctg
    14281 tttatgcggt tgccgcatct ttcttcacgg cggttatttt gatttcgacg caccactccg
    14341 gacgggcgag ccgagcttcc acgcaggcgc gggcgggcgt tctgccggcg gcaacccaag
    14401 cgtcccacac gccgttcatt tccgcatagt cgcccatatc gcgcagatag atgaccgcat
    14461 ccaaaacgtg cgctttgtcc gagccgcatt ccgccagcca gcggtcgatt tgggcaagca
    14521 cgtcggcagt ctgttcggca gccgtttcac cgttttcggg aaccatgccg gagaggaaaa
    14581 tcaagccgtt tgcgccgacg gcttcggaat agcggggcgt tgtgccgaaa tatcggatat
    14641 ccatatcggt ttccttcgat aaaggggata tatggtaaca ttgcgcttga ccgatttcca
    14701 tgttttgcat gacgaaaaat gagtaaacac acttatccga taacacctgc cgtgcgcgtt
    14761 ttgcgtgaaa acggcatcga atttgaacct tttacctatg cctatgagga acacggcggc
    14821 acggcgcagt ttgcccgact attcggcaaa gacgaacact tggtcattaa aaccattgtt
    14881 ttgcaagatg aaaacggtaa ggggctgatt gtcttgatgc acggcgacaa gcagatttca
    14941 acccgcaatc tggcgcggca tttgggtgcg aaacacattg aacccgccac gcccgtacag
    15001 gcaaacaagt ggacgggcta tctggtcggc ggcacaacgc cgttcggcat ccggacaaag
    15061 ttggatattt acgtcgaaca gtcggtgatg gatttggaaa ccatctatat caacggcgga
    15121 aaacgcgggt tcattatcgg catccgtccc agagatttaa atattttgaa cccgaaaaca
    15181 atacaggcgg cggtttgacg ggaaagtata aaggaacaat atggacaaag atttgtatgc
    15241 cgtattgggc gtgtcgccgc aggcgggagc ggacgaaatc aaacgcgcct accgcaagct
    15301 ggcgatgaaa tatcatcccg accgcaatcc gggcaatccg aaggcggaag aaaagttcaa
    15361 agaaatccaa cgggcttacg atacgctttc cgacctgtcg aaacggatgc aatacgacgc
    15421 gtccttcaga cggcatgagg aacgcgggcg gcaggaagag gcattccgcc gcgaacaggc
    15481 gcgcagggag cagttttacc gcgaacagat gcgccgcgaa caggcgttca gacaggcgtt
    15541 tgaacggcag gcatcacgtt cgtgccatac ttacgaaccg tccggcggcg gaagcgggcg
    15601 caactatgtc ctcgccgcct acatcctgtt cggtttgggt gcaatcatgc tgttcatgcc
    15661 catagtcggc gtgattttcg cctatatgcc catagtcggc gtgattctcg cctatatgaa
    15721 acggaacagt ttggacagca ttgtctatgc cgcacatacc gaatacctga ttaaaacctt
    15781 ttggcgcaca ttttggcttt atattttggg tgcgctgact gcccttttgg gtatcggcgt
    15841 gctgattatt attgcaacga acgtctggta tttctaccgc atcatcgccg gctttatccg
    15901 cttcaacggc ggcagggcgg ttgcacccga gaaatggata tagtatggct tacctgttaa
    15961 tcagcatcgt gttcagcgtg tcggtttcca ttttgctgaa aatggcaagg aagaaaaaaa
    16021 tcgacatcgc gcaggcggtc gccgtcaatt atgtggtcgc ggtcatactg accctgctgg
    16081 tattgaagcc ggatgtcggc aatatcggcg catttttgcc gacatggtgg ctgtttgccg
    16141 cattgggcgt gctgctgccg tccgtatttg tcattatggg caaatccgta gaatccgccg
    16201 gtatcgtcaa atccgacgcg gcgcagcgtt tgtcgctgtt tttgccgatt gttgccgcct
    16261 tgacgctgtt tggcgaaaaa ctcagcgaag gcagactaat cgggctgtgc ctcgcatttg
    16321 ccgcactgtt ctgcctgctt tggaaacaca gcggtggcaa aaaatcagga agcgcgtggc
    16381 ggcaggcggt attgctgctg ggcgtgtggg caggttacgg cattatcgat atcctgttca
    16441 aacagcttgc caaaagcggc acggcatttg cgggcaacct gctggttgca tttgtgctgg
    16501 cgggtgtgct gatgtttgcc tgcctgtttg ccaaatcggt cagatggcgt gttgagagtg
    16561 tggttggcgg catattcttg ggcggtttga actttatgaa catcgtaacc tacatcaccg
    16621 cgcaccaaat gatgaaggat aatccgacct tggtttttgc cggtatgaat atcggcgtga
    16681 ttgttttggg tacgctttcg ggcgcattgt tctttaagga aaaaatcaac acaatcaata
    16741 cggcgggaat cgtgttggca ctgtgttcta tcgcctgcct gttttattgg ggggaagtca
    16801 aggtactgtt cggcatataa tccggctgat tgatataaca aaatgccgtc tgaagcagca
    16861 tccctgcttc agacggcatt tgtctgcaac gttacagatg ggggttcatc aggttttcag
    16921 gagagaggat gcggttgagt tcttcttcgc tcaacagccc gcgttccaag acaacctcgc
    16981 gcacgccttt gccggtttgg gcgcagattt tgctgaccaa atcgccgttg tggtggccga
    17041 tatacggatt cagataagta accagcccga tggagttgaa aacgtaacgc tcgcatattt
    17101 cgcggttgac cgtaatgcct ttgacgcatt tgtcggacag gttgattgct gcattgccca
    17161 agagggaaat ggtttcaaac atacattggg cgatgaccgg ctccatcacg tttaattgca
    17221 gttgcccggc ttcggcggcg aaggtaatcg tcgtatcgtt gccgatgact ttgaagcaga
    17281 cttggttgac cacttcggga atcacgggat tgactttggc aggcataatg gaagaaccgg
    17341 cctgtaattc gggcaggttg atttctttca agccggcacg cgggccggaa gagagcaggc
    17401 gcaagtcgtt gcagattttg gagagtttga ccgccgtgcg cttcaatgcg ccgtgtacca
    17461 tcacatatgc gccgcagtcg gaggtcgcct caatcaggtt ttcggtcagt ttgcaaggca
    17521 agccgctgac ttcggagagt tttttgacca ccagttcggc gtagcctttg ggcgtgttca
    17581 cgcccgtgcc gattgccgtt gcgcccaaat tgacttctaa aagcagttgg cgcgtgcggt
    17641 cgaggttgag gatttcttct tccaacaaca cttggaaaga ttggaattct tgtcctgtag
    17701 tcattggcac ggcatcttga agctgggtgc ggcccatttt caaaacgtct ttaaattctt
    17761 cggctttggc ggcaaaggcg tttttcagaa cgaccagttt gtcgagcaat tcgccgatac
    17821 tgtaatacac ggcaaggcgg aagccggtag ggtaggcatc gttggtggat tggctggcgt
    17881 tgacgtgatc catcggattg acgatgtcgt agcggccttt ttcgtatccc aaggcttcca
    17941 atgcgaggtt ggcgatgact tcgttggtgt tcatattaac cgaagttccc gcaccgccct
    18001 gatacacgtc ggaggggaat tggtcgaggc agcggttgtt cagcagaact tcgtcgcaag
    18061 ccttttcaat ggcggcggcg atttcgggtt ttactgcacc caattcgccg tttgcctgcg
    18121 cggttgcttt tttcaccatc accatactgc ggacaaactg cggcacgtcg gaaatttttt
    18181 gtgtggagat tttaaagttt tcaatggcgc gcagagtgtg gatgccccaa tacacttcgg
    18241 cgggaatctc gcggtcgccc aataaatcgt gttcgatacg gacagtcatg ttttaccttt
    18301 gtaagtcgga taattaatat tgaaaaaatg cgccatcgga aagatgccgc cgcaggacga
    18361 acactatacc ggccggatga aattgtccat atcgtatgcc gtctgaaaac gggaaacgtt
    18421 gttttcgggt g
//
LOCUS       DAWKOG010000023        18282 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.023, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000023 DAWKOG010000000
VERSION     DAWKOG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18282
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.023"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            175..1818
                     /gene="pgi"
                     /locus_tag="WJK66_000620"
     CDS             175..1818
                     /gene="pgi"
                     /locus_tag="WJK66_000620"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ7578368.1"
                     /translation="MNAFTRAWYALERHYQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCRLAETADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     DAVYADGRDVLPEIRRELNRALEFAHSLDDGSYQGTTGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHITVHFAANADPACLDAVLCRLNPETTVFCVASKSFKTPETLLNAQAV
                     KAWYRGAGFSESETACHFCAVSADTAAAAAFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAACKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPVTAQGVEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPYNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            1788..2018
                     /locus_tag="WJK66_000621"
     CDS             1788..2018
                     /locus_tag="WJK66_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578369.1"
                     /translation="MPSERRRRGIKVPPPFCIDSGAEKAIPAARPIPKRQCSATARVL
                     LTNMRLRGTIAHSFQMNILPENTGKRPTKHLT"
     gene            2028..2849
                     /gene="dapD"
                     /locus_tag="WJK66_000622"
     CDS             2028..2849
                     /gene="dapD"
                     /locus_tag="WJK66_000622"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEZ7578370.1"
                     /translation="MSLQNIIETAFENRADITPTTVTPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQASPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            3004..3789
                     /gene="fabI"
                     /locus_tag="WJK66_000623"
     CDS             3004..3789
                     /gene="fabI"
                     /locus_tag="WJK66_000623"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEZ7578371.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(4033..5031)
                     /gene="ilvE"
                     /locus_tag="WJK66_000624"
     CDS             complement(4033..5031)
                     /gene="ilvE"
                     /locus_tag="WJK66_000624"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEZ7578372.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLVPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALIE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            5341..5655
                     /locus_tag="WJK66_000625"
     CDS             5341..5655
                     /locus_tag="WJK66_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEZ7578373.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINTDAVTFSYLPGQKFDLPLIVV
                     LFGAFVVGIVFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESAK
                     QP"
     gene            5666..6835
                     /gene="lapB"
                     /locus_tag="WJK66_000626"
     CDS             5666..6835
                     /gene="lapB"
                     /locus_tag="WJK66_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEZ7578374.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVIDQQPQSYDLNLTLGKLYRQRGENDKAINMHQTLLD
                     SPDTTGAKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGKMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDDQTYQFEIAQFYCELAQAALFKSNFDVARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKCEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDMNP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            6860..7276
                     /gene="gloA"
                     /locus_tag="WJK66_000627"
     CDS             6860..7276
                     /gene="gloA"
                     /locus_tag="WJK66_000627"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEZ7578375.1"
                     /translation="MRLLHTMLRVGNLEKSLDFYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDAYEACERVKRQGGNVVREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(7449..9989)
                     /locus_tag="WJK66_000628"
     CDS             complement(7449..9989)
                     /locus_tag="WJK66_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV family protein"
                     /protein_id="HEZ7578376.1"
                     /translation="MKNNRQIKLIAASVAVAASFQAHAGLGGLNIQSNLDEPFSGSIT
                     VTGEEAKALLGGGSVTVSEKGLTAKVHKLGDKAVIAVSSEQAVRDPVLVFRIGAGAQV
                     REYTAILDPVGYSPKTKSALSDGKTHRKTAPTAESQENQNAKALRKTDKKDSANAAVK
                     PAYNGKTHTVRKGETVKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHI
                     PNLNRIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAV
                     RPEPVPAANTAASETAAESAPQEAAASAIDTPTDETGNAVSEPVEQVSAEEETESGLF
                     GGSYTLLLAGGGAALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAADDGIEITFAE
                     VETPATPEPAPKNDVNDTLALDGESEEELSAKQTFDVETDTPSNRIDLDFDSLAAAQN
                     GILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTPEPESVAQTA
                     ENKPETVDTDFSDNLPSNNHIGTEETASAKPASPSGLAGFLKASSPETILEKTVAEVQ
                     TPEELHDFLKVYETDAVAETAPETPDFNAAADDLSALLQPAEAPSVEENITETVAETP
                     DFNATADDLSALLQPSEVPAVEENAAEIVADDLSALLQPAEAPAVEENVTETVAETSD
                     FHTAADDLSALLQPAEAPAVEENVTKTVAEIPDFNATADDLSALLQPSEVPAVEENAA
                     EITLETPDSNTSEADALPDFLKDGEEETVDWSIYLSEENIPNNADTSFPSESVGSDAP
                     SEAKYDLAEMYLEIGDRDAAAETVQKLLEEAEGDVLKRAQALAQELGI"
     gene            10297..10827
                     /locus_tag="WJK66_000629"
     CDS             10297..10827
                     /locus_tag="WJK66_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEZ7578377.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLSDAVWAKLTYMWVGFLIFMGIANWFVFTRFEAQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            10830..11120
                     /locus_tag="WJK66_000630"
     CDS             10830..11120
                     /locus_tag="WJK66_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEZ7578378.1"
                     /translation="MEYFMLLATDGENVYEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAEAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            11120..11398
                     /locus_tag="WJK66_000631"
     CDS             11120..11398
                     /locus_tag="WJK66_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ7578379.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            11447..12313
                     /locus_tag="WJK66_000632"
     CDS             11447..12313
                     /locus_tag="WJK66_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ7578380.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            12384..13142
                     /locus_tag="WJK66_000633"
     CDS             12384..13142
                     /locus_tag="WJK66_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ7578381.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDAILEENGVKP"
     gene            13216..13707
                     /locus_tag="WJK66_000634"
     CDS             13216..13707
                     /locus_tag="WJK66_000634"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEZ7578382.1"
                     /translation="MRAVIQKTVGAKVDVVSEAGTETCGKIDGGFVVLLGVTHSDTEK
                     DAHYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADATSGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            13738..14748
                     /gene="dusA"
                     /locus_tag="WJK66_000635"
     CDS             13738..14748
                     /gene="dusA"
                     /locus_tag="WJK66_000635"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEZ7578383.1"
                     /translation="MNDNTQTLPPRRLSVAPMLDWTDRHYRYMARQITRNTWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEEYGYNEVNLNCGCPS
                     PRVQKGAFGACLMNEVGLVADCLNAMQDAVKIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EEIAQHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTRSPIEYADLVQRLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKNARTWRRMLSDATLLKDNDGGLILEAWKEVERANM
                     RE"
     gene            complement(14818..15705)
                     /gene="gluQRS"
                     /locus_tag="WJK66_000636"
     CDS             complement(14818..15705)
                     /gene="gluQRS"
                     /locus_tag="WJK66_000636"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEZ7578384.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAVASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVAYQSRRYALYEETLCRLKTAGLVYPCHCSRK
                     DWQAGARRGADGFVYNGRCRHPGQRPAPQGKQPAWRIRVPDRDIGFSDGIVGGYAQNL
                     AGDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLDVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLNLPEAPEADRPAELLD
                     WAVAHWDMGKVPKHAITAP"
     gene            complement(15722..16012)
                     /locus_tag="WJK66_000637"
     CDS             complement(15722..16012)
                     /locus_tag="WJK66_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578385.1"
                     /translation="MAKALEIISPNEIYSDLIFKDPVPPHTIYTELMKLVSRNASNER
                     IADRAFDFFSSGLMDGSATEEQYNALYDLTLLEEPGMELNKDEIMALINSLK"
     gene            complement(16166..17221)
                     /gene="tal"
                     /locus_tag="WJK66_000638"
     CDS             complement(16166..17221)
                     /gene="tal"
                     /locus_tag="WJK66_000638"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEZ7578386.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKQQDLTPKQRYETMAVADVRAACGVCLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIHVVASFFISRVDSALDTTLPDRLK
                     GKIAIALAKAAYQDWEQYFTAPEFAALEAQGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGVEEAQAQLAETAALGIDVETLATRLQED
                     GLKQFEEAFEKLLAPLV"
     gene            17317..>18282
                     /locus_tag="WJK66_000639"
     CDS             17317..>18282
                     /locus_tag="WJK66_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEZ7578387.1"
                     /translation="MAENGKYLDWAREVLHTEAEGLREIAAELDENFALAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAAR"
BASE COUNT         4025 a         4737 c         5288 g         4232 t
ORIGIN      
        1 tgcggctcgc cgccttgtcc tgatttttgt taattcacta tattttcgcc atatggaaat
       61 aaggtgctat tggacgcggc gggcggtgtt ccggagattc gccaaagccg ctgccgtttg
      121 ttaaactaca ttctgctaca ttttaatccg gttctgaaaa atcaaggaaa acagatgaat
      181 gcttttaccc gtgcatggta tgcgctcgaa cgccattatc aggatacgcg tcatgtcctt
      241 ttgcgcgacc gctttgcctg cgaacccgac cgttttgagc gtatgcatga gcgtttggac
      301 gggatgttgt tcgattacag caaaaaccgt ttgggcgaag atacgctgca actgctctgc
      361 cgtcttgcgg agacggcgga tttggaaggg aaaatgcgtg ctttgcggac gggtgcgaaa
      421 gtcaacggca gcgaggggcg tgccgcgctg catacggctt tgcgcctacc cgacggtgcg
      481 gatgccgttt atgcggacgg cagggacgtg ttgcccgaaa tccgccgcga gttaaatcgt
      541 gcgttggagt ttgcacacag tttggacgac ggttcgtatc aggggacaac cggaaaacgg
      601 attacggatt ttgtccacat cggcataggc ggatccgacc tcgggccggc aatgtgcgtg
      661 caggcacttg agccgttcag acggcatatc accgtccatt ttgccgccaa cgccgatcct
      721 gcctgcctgg atgcggtttt atgccgtctg aaccccgaaa cgacagtgtt ttgcgttgcc
      781 agcaagtcct tcaaaacacc ggaaaccctg ctcaatgcac aggcagtcaa ggcgtggtat
      841 cgcggtgcgg ggttctcgga atccgaaacg gcgtgccatt tttgcgcggt gtctgccgac
      901 actgcggcag ctgcggcttt tggtatcgcg gcggaacgcg tgtttgcgat gtacgactgg
      961 gtgggcggac gctattccgt ctggtcgccc gtcggtttgc ccgtgatggt tgcggtcggc
     1021 ggggcgcgtt tccgcgagtt gttggcgggg gcgcacgcga tggacagcca ttttttccac
     1081 acgccgccgc gccgcaacat tcccgttctg atggcactga ttgccgtgtg gtataacaat
     1141 ttccaacacg cggacgggca gaccgccgtt ccgtacagcc acaacctgcg cctgctgccg
     1201 gcgtggctga accagctcga tatggagagt ttgggcaaaa gccgcgcttc agacggcagt
     1261 cccgccgcgt gcaaaacggg cggcatcgtg ttcggcggtg aaggggtcaa ctgccagcac
     1321 gcctatttcc aactgctcca ccaaggcacg cgcctgattc cctgcgactt tatcgtcccg
     1381 gtgacggcgc agggcgtgga ggacggacgc agccgtttta ccgttgccaa cgcctttgcc
     1441 caagcggaag ccctgatgaa gggcaaaacc ttggacgaag cacgcgccga actggcagat
     1501 ttgcccgaag cggaacgcga acgcctcgcg ccgcataaag agttccccgg caaccgcccc
     1561 agcaacagca tcctgctcga ccgcctcacg ccctacaatc tgggtatgct gatggcggct
     1621 tacgaacaca aaaccttcgt ccaaggcgcg atatggaacg tcaacccctt cgatcagtgg
     1681 ggggttgagt acggcaaaca gttggcgaaa accatcatcg gcgaactgga aggcggcacg
     1741 tccgtacacg atgcctcgac cgaagggctg atggcgtttt accgcgaatg ccgtctgaaa
     1801 ggcggcggcg cggcataaaa gtgccgccgc cgttctgtat tgattcgggt gcggaaaagg
     1861 caatacctgc cgcccgcccg attccgaaac gccaatgttc ggcaaccgcc cgcgtattgc
     1921 tgacgaatat gcgtttgcgt ggcacaatag cgcattcatt tcaaatgaac atactgcccg
     1981 aaaataccgg caaacgtccc acgaaacatc tcacataagg aaacattatg tctttgcaaa
     2041 acatcatcga aaccgccttt gaaaaccgcg cggacatcac cccgaccacc gttactcccg
     2101 aagtcaaaga agccgtgttg gaaaccatcc gccagctcga ttccggcaaa ttgcgcgtcg
     2161 ccgaacgctt gggcgtgggc gagtggaaag tcaacgaatg ggcgaaaaaa gccgtgttgc
     2221 tgtccttccg catccaagac aacgaagtcc tcaacgacgg cgtgaacaaa tacttcgaca
     2281 aagtgccgac caagtttgcc gattggtctg aagacgagtt caaaaacgca ggcttccgcg
     2341 ccgttccggg tgcggttgcc cgacgcggca gctttgttgc caaaaatgtc gtgctgatgc
     2401 cgtcttatgt caacatcggc gcatacgttg acgaaggcgc gatggttgat acttgggcga
     2461 ccgtcggctc ttgcgcgcaa atcggtaaaa acgtccactt gagcggcggt gtcggcatcg
     2521 gtggcgtact cgaacccctg caagccagcc cgaccattat tgaagacaac tgcttcatcg
     2581 gtgcgcgttc tgaaatcgtt gagggcgtga ttgtcgaaga aggcagcgtg atttcgatgg
     2641 gcgtgttcat cggtcaatcc accaaaatct tcgaccgcac caccggcgaa atctaccaag
     2701 gccgcgtacc ggcaggttcg gtcgtcgtgt ccggcagtat gccttccaaa gacggcagcc
     2761 acagccttta ctgcgccgtc atcgtcaaac gcgtggacgc gcaaacccgt gcgaaaacca
     2821 gcgtgaacga attgttgcgc ggcatctgat gccttaaacc gtatttgaaa cgtccgatgc
     2881 cgtctgaaat ccgcttcgga cggcattgcc gtttgcatgc tgcaacgtga aaacacagaa
     2941 acagggacaa tttgctataa tcaacggttt agaatgaacc gaacactatt tgaaggatac
     3001 aaaatgggtt ttctgcaagg caaaaaaatt ctgattaccg gcatgatttc cgagcgttcc
     3061 atcgcttatg gcatcgccaa agcctgccgc gaacaaggcg cggaactggc gtttacctac
     3121 gttgtggaca aactggaaga gcgcgtccgc aaaatggcgg cggaattgga ttccgaactc
     3181 gtattccgct gcgatgtcgc cagcgacgac gaaatcaacc aagtgttcgc cgacttgggc
     3241 aaacattggg acggcttgga cggtttggtg cattccatcg gttttgcgcc gaaagaagcc
     3301 ttgagcggcg acttcctcga cagcatcagc cgcgaagcgt tcaacaccgc acacgaaatt
     3361 tccgcataca gcctgcccgc gctggcaaaa gccgcccgtc cgatgatgcg cggcagaaat
     3421 tccgccatcg tcgccctgag ctacttgggc gcggtacgcg cgattccgaa ttacaacgtg
     3481 atgggtatgg caaaagccag ccttgaggca ggcatccgct ttaccgccgc ctgtctgggc
     3541 aaagaaggca tccgctgcaa cggcatttcc gcaggtccga tcaaaacgct cgccgcctcc
     3601 ggcattgccg atttcggcaa actcttggga cacgtcgccg cccacaatcc gctgcgccgc
     3661 aacgttacca tcgaagaagt cggcaatacc gccgccttcc tgctgtccga cctgtcgtcc
     3721 ggcattaccg gcgaaatcac ttatgttgac ggcggttaca gcatcaatgc cttgagcacc
     3781 gagggataat ccgccgtttt caaatccgtg cgccgtccgt gccgcatatc ggtttcgggc
     3841 ggcgttttgc cgtctgaagc gtatttctag ggaaatgccc gacttacggc aggcgggatg
     3901 ggaaatgcgg acgcttgttt taaccgattg cctttgtgcc gacttgctgc aggtgcagcg
     3961 gaaacggttc ggatgcgaaa atgccgtctg aaacgccaaa cgggtttcag acggcatttt
     4021 ttatttaaag catcagcaca cttcaaccag ccagccgtat ttgtcttccg ccaaaccata
     4081 ctggatgtcg gtaatcgcct tacggatggc atagccgcgt tcttggcttt tcacttcgat
     4141 ttctttgccg ccgatgacga aggaagtaac gggcgagatg acggctgccg taccggtcag
     4201 aatcgcctcc gcaccgtttt ccaccgcagc tttgagttcg tcaaccgtga aattgcgttc
     4261 gctgacggta tagcccaaat ctttggcaac cgtcagtacg gaatcgcggg ttacgccgtg
     4321 caaaaattcg tcggtcagcg gtttggtaat gatttcatcg ccgttaatca ggataaagtt
     4381 ggacgcgccg gtttcctgca cgtcgccgtt cgggcagaag aggacttggt ttgcgccgta
     4441 ttcggctttc gccttcagca cccagtgcat ggcggaagcg tagttgccgc cgcatttgac
     4501 gcggcccata tgcggggcgc agcggatgtg ttcggtttcc actaaaattt tgacgggcga
     4561 gccgactttg aaatagtcgc cgacggggga agccaaaata tacagcaggg cggtttcgga
     4621 aggagaaccg gccttgccga tgacgggatc ggtaccgatt aaggtcgggc gcaggtacag
     4681 ggcggcaggc gcatcgggaa tttcgtcggc ggcacgtttg accaattcga ttagcgcgtc
     4741 gagataggct tgggtttcgg ggcggggcag gtgcaaaatg tccgcacttt gccgcatacg
     4801 cgcgatattg gcagtcggac ggaacagtac gattttaccg tctgcctgac ggaaggcttt
     4861 cagtccctcg aaacattcgc tgccgtagtg cagggcgtgc gcacccggta cgagggagag
     4921 gtcttgggaa gattgccatt cggtcggctg ccatttgcct tcgcggtagg cgaggacggg
     4981 catttgactg tgaaaaacgc tgccgaatac ggcgggtacg ggtctgctca tgatgtaaag
     5041 cctttcttat tctgatatgt ttcaatgaac ggtttgaatt tgaagattgt aaagatacgc
     5101 ctgcaaacag ggttttgaca agtgcgcggc gggtttttct gtcgatgcgg tgtccaatcc
     5161 gttatttttc aaatggaaag gaacggtgta tttggtaaaa ttgtcggcaa tcgcatactc
     5221 cgtatgtcgt ccgaacacgc tgccgcatcc tatccgaaac cgtgcaaatc gtttaaacta
     5281 gcgcaatctt ggttcagagt gcgaagctgt ccgggcggcg tttttattta cggagcaaac
     5341 atgaaactta tctataccgt catcaaaatc attatcctgc tgctcttcct gctgcttgct
     5401 gtcattaata cggatgccgt taccttttcc tacctgccgg ggcaaaaatt cgatttgccg
     5461 ctgattgtcg tattgttcgg cgcatttgta gtcggtattg tttttggaat gtttgccttg
     5521 ttcggacggt tgttgtcgtt acgtggcgag aacggcaggt tgcgtgccga agtaaagaaa
     5581 aatgcgcgtt tgacggggaa ggagctgacc gcaccaccgg cgcaaaatgc gcccgaatct
     5641 gccaaacagc cttgagaaag ccgatatgga caatgaattg tggattatcc tgctgccgat
     5701 tatccttttg cccgttttct tcgcgatggg ctggtttgcc gcccgcgtgg atatgaagac
     5761 tgtattaaag caggcaaaaa gcataccgtc gggattttat aaaagtctgg atgccttggt
     5821 tgaccgcaac agcggacgtg ccgccaggga attggcagag gtcatcgatc agcagcctca
     5881 gtcatacgac ctgaacctca ccctaggcaa gctttaccgt cagcgtggcg aaaacgacaa
     5941 agccatcaat atgcaccaaa cattgcttga ctctcccgat acaaccggag ccaagcgcgc
     6001 gcgcgtcctg tttgaattgg cgcaaaacta ccaaagtgcg gggttggtcg atcgtgccga
     6061 acagattttt ttggggctgc aagacggtaa aatggcgcgt gaagccagac agcacctgct
     6121 caatatctac caacaggaca gggattggga aaaagcggtt gaaaccgccc ggctgctcag
     6181 ccatgacgat cagacctatc agtttgaaat cgcccagttt tattgcgaac ttgcccaagc
     6241 cgcgctgttc aagtccaatt tcgatgtcgc gcgtttcaat gtcggcaagg cactcgaagc
     6301 caacaaaaaa tgcacccgcg ccaacatgat tttgggcgac atcgaacacc gacaaggcaa
     6361 tttccctgcc gccgtcgaag cctatgccgc catcgagcag caaaaccatg catacttgag
     6421 catggtcggc gagaagcttt acgaagccta tgccgcgcag ggaaaacctg aagaaggctt
     6481 gaaccgtctg acaggatata tgcagacgtt tcccgaactt gacctgatca atgtcgtgta
     6541 cgagaaatcc ctgctgctta agtgcgagaa agaagccgcg caaaccgccg tcgagcttgt
     6601 ccgccgcaag cccgacctta acggcgtgta ccgcctgctc ggtttgaaac tcagcgatat
     6661 gaatccggct tggaaagccg atgccgacat gatgcgttcg gttatcggac ggcagctaca
     6721 gcgcagcgtg atgtaccgtt gccgcaactg ccacttcaaa tcccaagtct ttttctggca
     6781 ctgccccgcc tgcaacaaat ggcagacgtt taccccgaat aaaatcgaag tttaaccacc
     6841 accgaaagga acacaaaaaa tgcgcttact ccatactatg ctccgcgtgg gcaatctcga
     6901 aaaatccctc gatttctacc aaaacgtttt gggtatgaaa ctgctccgcc gaaaagatta
     6961 tcccgaaggc agatttaccc ttgccttcgt cggttacggc gatgaaaccg acagcacggt
     7021 tttggaactg acgcataact gggatacgga acgatacgac ttgggcaacg cctacggaca
     7081 catcgcggtt gaagtggacg atgcctacga agcctgcgaa cgtgtgaagc ggcagggcgg
     7141 aaacgtcgtc cgcgaagccg gcccgatgaa acacggcaca accgtgatag ccttcgtcga
     7201 agaccccgac ggatacaaaa tcgagttcat tcaaaagaaa agcggcgacg attcggttgc
     7261 ctatcaaact gcctgatacc gccgccgcca atgccgtctg aagcctttag ggggttcaga
     7321 cggcattttg ttgtcggcgg tctgctgttt gagcctgtgc cgggtcaaac tttatccgtt
     7381 acaccgataa ggcaaaaaaa gatgccgtct gaaacggcat ccttgatctg cgaaagggca
     7441 gttgggaatc aaatacccaa ttcctgcgcc aatgcttggg cacgtttgag tacgtcgcct
     7501 tccgcttctt ccagcaattt ctgcactgtc tcggcagcgg catcgcggtc gccgatttcg
     7561 agatacattt cggcaaggtc gtatttcgct tcggaaggcg cgtcagaacc tacagattcc
     7621 gaagggaaac tggtatctgc attatttggg atattttctt ccgagaggta gatgctccaa
     7681 tctaccgttt cctcctcgcc gtctttcagg aagtcgggca aagcgtctgc ctcagaggtg
     7741 ttggaatcag gcgtttccaa agtgatttcc gctgcatttt cctcaacggc cggtacttca
     7801 gaaggttgaa gtaatgcgga caaatcgtct gcggtggcgt tgaaatcagg tatttcggca
     7861 acggttttcg ttacattttc ctcaacggcc ggtgcttcag caggttgcaa cagtgcggac
     7921 aaatcgtctg cggcggtgtg gaagtcggac gtttcggcaa cagtttccgt tacattttcc
     7981 tcaacagccg gtgcttcagc aggttgcaac agtgcggaca aatcatcggc aacgatttcc
     8041 gctgcatttt cctcaacggc aggtacttca gaaggttgaa gtaatgcgga caaatcgtct
     8101 gcggtggcgt tgaagtcggg tgtttcggca acggtttccg ttatattttc ctcaacggac
     8161 ggtgcttcgg caggttgaag caatgcggac aaatcgtctg cggcggcgtt gaaatcgggc
     8221 gtttcaggcg cagtttccgc gacggcatcg gtttcgtaca ctttcaggaa atcgtgcaac
     8281 tcttccggtg tttggacttc ggcaactgtt ttttccaaga tggtttcggg cgaggaagcc
     8341 ttcaggaagc ctgccagtcc ggagggtgag gcaggttttg cggaagctgt ttcttctgtg
     8401 ccgatatggt tgtttgaggg caggttgtcg gagaaatcgg tatcgacggt ttccggtttg
     8461 ttttcggcag tttgggcgac agattccggt tcgggcgtgt cgatgacgat ttcgacaggg
     8521 ttgtacgggt tgaaggtctc gggctcgtac acgctgtctg tggattcgat ggcgttccaa
     8581 tcggcatccg cgcgtttttg ggtttcttca tcctgcgtaa gtgcgccgga taaaatgccg
     8641 ttttgcgcgg ctgccaggct gtcgaaatcc aagtcgatgc ggttggaagg cgtatcggtt
     8701 tcgacatcga acgtttgttt tgccgacaac tcttcttcag attccccatc taaggcaagt
     8761 gtgtcgttta catcgttttt cggagcgggt tcgggcgttg ccggagtttc gacttcggca
     8821 aaggtgattt ctatgccgtc gtctgccgcg tcgtcaaggt caggctcttc ctcagggacg
     8881 gattcttcgg tacggcgcgc gcgtttggat tgggcaaggc gcaaaagcag cagcagggcg
     8941 atcaatgccg cgcctccgcc ggcaagcagc aaggtgtacg aaccgccgaa cagtccgctt
     9001 tcggtttctt cttcggcaga aacctgttcg acaggttcgg aaacggcgtt accggtttcg
     9061 tcggtcggcg tgtcgatggc agaagcggcg gcttcttggg gggcggattc ggcagcggtt
     9121 tccgatgcgg cagtatttgc agcgggtaca ggttcgggtc gaacggccgg tttttccgct
     9181 tttgcttcgg gcgcggcaac ttttgcttca ggtttttcaa ccggtttctc taccgttgcc
     9241 tgtttggacg gttcggacgg catggatgcg gtttcggctt tgggtttcgc cgtttgcggt
     9301 ttgggttgtt ccgctttgat cctgttcaga ttcggaatgt gaagcacgct gcccgcacgc
     9361 agtctgccgt gtgcggaaac atttgggttt gccttcagca gcgcatcggc aacctgttcg
     9421 agcgtcaggt gtttcggacg gatggcggcg gcaatctgtt tgaccgtttc gcctttgcgg
     9481 acggtatggg ttttgccgtt gtacgccggt ttgacggctg cgttcgcgct gtctttttta
     9541 tcggttttgc ggagggcttt ggcgttttga ttttcttggg actctgctgt cggagcggtt
     9601 ttgcggtgtg tcttgccgtc tgaaagtgca gatttggttt tgggcgagta gccgacagga
     9661 tcgaggatgg cggtgtattc gcgtacctgt gcgcctgcgc cgatgcggaa taccaggacg
     9721 ggatcgcgga ctgcctgttc ggaagaaacg gcaatgacgg ctttgtcgcc caacttgtgg
     9781 actttggcgg tcaggccttt ttcggaaacg gtaacgctgc cgccgcctag cagggctttg
     9841 gcttcttcgc cggttacggt aatgctgccg gaaaagggtt cgtcaaggtt ggactggata
     9901 ttcagtccgc ccagtccagc atgtgcctga aaggatgcgg caactgcgac ggaggcggca
     9961 atcagtttga tttgtctgtt gtttttcaag atgtatcccc tgtgggttgg cggctgaata
    10021 cggtttgacc gcgtacagtc tgtaaatttc gtcatcatcg ggcatcggcg gggcagtcgg
    10081 ccggcgggca tttaatatgt gaatgtaccg accgccgcca cattttaaac ggcaatcatt
    10141 cgccgttttt acaaattatg acatatctcc atcttttttc aaaaacatct gtgcatattt
    10201 gcatcaatca aaacaaaatt tgttggtttt gcaggtgcaa aaacagggtt ctgcctgtat
    10261 gattagcgtt tatttgattt gctttctcat ttggatatga aattcgtcag cgaccttttg
    10321 tccgtcatcc tgtttttcgc cacctatacc gttaccaaaa acatgattgc cgcaacggcg
    10381 gtcgcattgg ttgccggtgt ggttcaggcg gcttttctgt attggaaata taaaaagctg
    10441 gatacgatgc agtgggtcgg attggtgctg attgtggtat tcggcggcgc aaccattgtt
    10501 ttgggcgaca gccgcttcat tatgtggaag ccgagcgttt tgttttggct gggcgcgctg
    10561 ttcctgtggg gcagccacct cgccggtaaa aacggcttga aggcgagtat cggcagggag
    10621 attcagcttt cagatgccgt atgggcgaaa ttgacgtata tgtgggtcgg ttttctgatt
    10681 tttatgggta ttgccaactg gtttgtgttt actaggtttg aagcgcaatg ggtcaactat
    10741 aaaatgttcg gctcgactgc actgatgctt gttttcttta ttattcaggg tatttatctg
    10801 agtacctgtc tgaaaaagga ggattgactg tggaatattt tatgttgctg gcaacagacg
    10861 gggagaatgt gtatgaagcg cgtatggcgg cacgtcccga acacctcaaa cggctggaga
    10921 cgctgaagtc ggaaggccgg ctgttgacgg caggcccgaa tcctttgccg gaggactcca
    10981 accgcgtttc gggcagtttg attgtggcgc agttcgagtc tttggatgcg gcagaggcgt
    11041 gggcggaaga cgatccctat gttcatgcag gcgtgtacag cgaagtgctg atcaagccgt
    11101 ttaaagcggt gttcaaataa tgccggccgt cgatttgatc cgcgaacgcc tgcagacgct
    11161 cgatccgctg gtgttggaaa tcggcgatga gagccatctg cacaaaggac acgcgggcaa
    11221 taccggcggc ggacattatg ccgttttggt cgtcagcggc cgttttgaag gcgtaagccg
    11281 cctgaaccgc cagaaaacgg tcaaatcgct gctcaaagat ttgttttcag gcggcatgat
    11341 tcacgcgctc ggcatccggg cggctacccc tgacgagtat ttccatacgg cggactgaat
    11401 gaagtctgcc cgaacatttc aatttaaaat ttaaagagag aagattatga aagcaaaaat
    11461 cctgacttcc gttgcactgc ttgcctgttc cggcagcctg tttgcccaaa cgctggcaac
    11521 cgtcaacggt cagaaaatcg acagttccgt catcgatgcg caggttgccg cattccgtgc
    11581 ggaaaacagc cgtgccgaag acacgccgca actgcgccaa tccctgctgg aaaacgaagt
    11641 ggtcaacacc gtggtcgcac aggaagtgaa acgtctgaaa ctcgaccggt cggcagagtt
    11701 taaaaatgcg cttgccaaat tgcgtgccga agcgaaaaag tcgggcgacg acaagaaacc
    11761 gtccttcaaa accgtttggc aggcggtaaa atatggcttg aacggcgagg catacgcgct
    11821 gcatatcgcc aaaacccaac cggtttccga gcaggaagta aaagccgcat atgacaatat
    11881 cagcggtttt tacaaaggta cgcaggaagt ccagttgggc gaaatcctga ccgacaagga
    11941 agaaaatgca aaaaaagcgg ttgccgactt gaaggcgaaa aaaggtttcg atgccgtctt
    12001 gaaacaatat tccctcaacg accgtaccaa acagaccggc gcgccggtcg gatatgtgcc
    12061 gctgaaagat ttggaacagg gtgttccgcc gctttatcag gcaattaagg acttgaaaaa
    12121 aggcgaattt acggcaacgc cgctgaaaaa cggcgatttc tacggcgttt attatgtcaa
    12181 cgacagccgc gaggtaaaag tgccttcttt tgatgaaatg aaaggacaga ttgccggcaa
    12241 ccttcaggcg gaacggattg accgtgccgt cggtgcactg ttgggcaagg caaacatcaa
    12301 acctgcaaaa taattctgaa aacgggatat ggcggcaaga cgttcagaca ggcgttttgc
    12361 cgccgcgcag gacagggaat accatgaaac agaaaaaaac cgctgccgca gttattgctg
    12421 caatgttggc aggttttgcg gcagccaaag cacccgaaat cgacccggct ttggtggata
    12481 cgctggtggc gcagatcatg cagcaggcag accggcatgc ggagcagtcc caaaaaccgg
    12541 acgggcaggc aatccgaaac gatgccgtcc gtcggctgca aactttggaa gttttgaaaa
    12601 acagggcatt gaaggaaggt ttggataagg ataaggatgt ccaaaaccgc tttaaaatcg
    12661 ccgaagcgtc tttttatgcc gaggagtacg tccgttttct ggaacgttcg gaaacggttt
    12721 ccgaaagcgc actgcgtcag ttttatgagc ggcaaatccg catgatcaaa ttgcagcagg
    12781 tcagcttcgc aaccgaagag gaggcgcgtc aggcgcagca gctcctgctc aaagggctgt
    12841 cttttgaagg gctgatgaag cgttatccga acgacgagca ggcttttgac ggtttcatta
    12901 tggcgcagca gcttcccgag ccgctggctt cgcagtttgc agcgatgaat cggggcgacg
    12961 ttacccgcga tccggtcaaa ttgggcgaac gctattatct gttcaaactc agcgaggtcg
    13021 ggaaaaaccc cgacgcgcag cctttcgagt tggtcagaaa ccagttggaa caaggtttga
    13081 gacaggaaaa agcccgcttg aaaatcgatg ccattttgga agaaaacggt gtcaaaccgt
    13141 aatggcattt ccaataccga tgccgtctga agccttttca gacggcattg cacgttcagg
    13201 taaggaggac ggcttatgcg tgcggtcata cagaaaacgg taggtgcaaa ggtggatgtc
    13261 gtgtccgaag ccggcacgga aacctgtggc aaaatcgacg gcgggtttgt cgtgttactc
    13321 ggcgtaacgc atagcgacac agaaaaagat gcgcactata tcgccgacaa aatcgcccat
    13381 ttgcgcgtgt ttgaagacga agcgggcaag ctgaacctgt ctttgaaaga tgtcggcggc
    13441 gcggtgctgc tggtgtcgca gtttacactt tatgccgacg cgacaagcgg gcggcggcct
    13501 tcgttttcgc aagccgcacc tgcagaacag gcgcagcagc tttacctgcg aacggcggaa
    13561 ctgttgcgcg gacacggaat tcatgtcgaa acagggcgtt tccgcacgca tatgcaggtg
    13621 tcgctctgca acgacgggcc ggtaaccata ctgctggact ctttcatgac gcggatttcc
    13681 ccaaaaatga aggttgttcc ggattgaaat tgaatccgca atggtgaaat atcgacaatg
    13741 aacgacaaca cgcaaaccct tcctccccgc cgcctgtccg tcgccccgat gttggactgg
    13801 acggacaggc actaccgtta tatggcgcgc cagattaccc gcaatacttg gctgtacagc
    13861 gaaatggtca acgccggcgc gattgtttac ggcgacaaag accgcttttt gatgtttaac
    13921 gaaggcgagc agcccgtcgc cctgcaactg ggcggcagcg acccgtccga tttggcgaaa
    13981 gccgccaaag ccgccgaaga atacggctac aacgaggtca acctcaactg cggctgcccc
    14041 agtccgcgcg tccagaaagg cgcgttcggc gcgtgtctga tgaacgaagt cgggctggtt
    14101 gccgactgcc tcaacgccat gcaggatgcg gtcaagattc ccgttaccgt caaacaccgc
    14161 atcggtgtgg acaggcagac cgaataccaa accgttgccg atttcgtcgg cacgctgcgc
    14221 gacaaaaccg cctgcaaaac ctttatcgtc cacgcacgta acgcgtggct ggacggtctt
    14281 tcacccaaag aaaaccgcga cgttccgccc ttgaaatacg attatgttta ccgcctcaag
    14341 caagagtttc ccgggctgga aatcatcatc aacggcggca tcacgaccaa tgaggaaatc
    14401 gcgcaacacc tgcaacacgt tgacggcgtg atggtcgggc gcgaggcgta ccacaacccg
    14461 atggtgatgc gcgaatggga caggctgttt tacggcgata cccgcagccc gattgaatac
    14521 gccgatttgg tgcagcgtct ctacacatac agtcaagccc aaatccaagc cggacgcggc
    14581 acaatcttgc gccacatcgt ccgccacagc ctcgggctga tgcacggtct gaaaaatgcc
    14641 cgcacttggc ggcgtatgct ttccgatgca acgctcttga aagacaacga cggcggcctg
    14701 attctcgaag cgtggaaaga agtcgaacgg gcaaatatgc gcgaataggg cggggctgtg
    14761 tgtatgaaat gccgtctgaa ggtttcagac ggcatttgtg cgtttgtcgg gcggtgttta
    14821 gggggcggta atggcgtgtt tcggcacttt gcccatatcc cagtgtgcca ccgcccagtc
    14881 gagcagttcg gcagggcggt cggcttccgg tgcttcgggc aggttgaggt aacggaacac
    14941 ttggcggagg agttgttcgc ggcggtttaa atccaatgcg ggggcgagcg tctgtttcga
    15001 ccatttctgc ccttgtgcgt tggtcagcag cggcaggtgg gcatattgcg gtgtcggaac
    15061 gtccaaacac tgctgcaaat agatttggcg cggcgtagaa acgagcaggt cttgtccgcg
    15121 gacgatgtgg gtaacgccct gttcggcatc gtcggcaacg acggcgagtt ggtatgccca
    15181 gtaaccgtct gcacgaagca ggacgaaatc gccgatgtcg ccggcgaggt tttgggcgta
    15241 accgccgacg atgccgtctg aaaagccgat gtcgcggtcg gggacgcgga tgcgccacgc
    15301 aggctgtttg ccttgcggtg cagggcgttg accggggtgg cggcagcgtc cgttatagac
    15361 gaacccgtct gcgccccgcc ttgccccggc ctgccagtct ttgcggctgc aatggcaggg
    15421 atagaccagt ccggcggttt tcaggcggca tagggtttct tcatacaggg cgtaacggcg
    15481 gctctgatag gcgacttctc cgtcccactc gaatccgaat gcctcaagcg tgtgcaggat
    15541 atggcttgcc gcccccggca tttcgcgcgg cggatcgagg tcttccatgc ggaccagcca
    15601 tttgccgccg tttgagcgtg catcggcata ggaagcgacg gcggtcagca gcgagccgat
    15661 gtggagcagc ccggtcgggc tgggggcaaa acgtcctgtg tacatatctg gtacagcccc
    15721 tttatttaag actattaatc aaagccatta tctcatcttt attcagttcc atcccgggtt
    15781 cttcaagtaa ggttaaatca tatagggcat tatattgctc ttcggtagct gaaccatcca
    15841 taaggccgga cgagaaaaaa tcgaaggctc tatctgcgat tctctcatta cttgcatttc
    15901 tactaaccag tttcatcaat tctgtatata ttgtatgggg aggtaccgga tccttaaaaa
    15961 tcagatctga ataaatttca ttgggtgaaa tgatttcgag tgcttttgct atggttctat
    16021 ttccttttgt gttggcgggt aatgtcgtgc attaacttct tgcccattaa tatttttagg
    16081 gcgaatcctt gatatgccgc actgtgtccg gtcaaaaggg cgatgccgtc tgaaagcctt
    16141 tcagacggca tcggggatga aaagtttaaa ccaacggtgc gagcagtttt tcaaacgctt
    16201 cttcaaactg tttcaaaccg tcttcctgca aacgcgttgc caaggtttcg acatcgatgc
    16261 cgagcgcggc ggtttcggcg agctgcgctt gtgcttcttc tacgccttcg gtcagcgtgg
    16321 ctttggctgt gccgtggtcg ataaaggctt tgagcgtggc atcgggaacg gtgttgacgg
    16381 tgtgcgcgcc gatcaggctg tcaacgtaga gcgtgtcggg ataggctggg tttttcacgc
    16441 cggtagatgc ccataaaagc tgcacgcggt ttgcgccttg ggcttccagt gcggcaaatt
    16501 cgggggcggt gaaatattgt tcccaatctt gataggcggc tttggcaagg gcgatggcga
    16561 ttttgccttt gagccggtcg ggcagtgttg tgtccagcgc actgtccaca cgcgagatga
    16621 agaagctggc gacgacgtgg atatgggcaa cgctttgtcc ggctgccaag cgtttggcaa
    16681 tgccgcgcgc gtaggcggcg taggctttga gggtttgggc gcgtgagaac agcagggtca
    16741 ggttcacgct gatgccgtct gaaacgaggg tttcgagcgc atcgatgcct gcgtcggtgg
    16801 caggcacttt aatcatcgcg tttttgcacc cgatggcggc gtagaggcgg cgcgcttctt
    16861 caaccgtgcc ttgcgcgtct ttggacaatt cgggcgaaac ttcgaggctg acgaagccgg
    16921 ttttgccgcc ggtggattcg tgttcggcaa gacagacgcc gcaggcggca cgcacatcgg
    16981 caaccgccat cgtttcgtag cgttgtttgg gggtcaggtc ttgctgcttg agggcggcaa
    17041 cctcgtcggc ataaagcgcg tcgccggcga aggctttttg gaaaatggcg ggattggaag
    17101 ttacgccgca cacgccttgt ttcaacattt gcgccaattc gccgctttgc accagcgagc
    17161 gggaaaggtt gtccagccag atttgttgtc ctaatgcttt aacgtccgat aaaatagtca
    17221 tttctgattc ctttggatgg atggcggggt ttgaatgctt atgctacccc gattcggaaa
    17281 ttttgggtag ttttgttaca gcaaaggcgg atggcaatgg cagaaaacgg aaaatatctc
    17341 gactgggcgc gcgaagtgtt gcacaccgaa gcggaaggct tgcgcgaaat tgcagcggaa
    17401 ttggacgaaa acttcgccct tgcggcagac gcgttgttgc actgcaaggg cagggttgtt
    17461 atcacgggca tgggcaagtc gggacatatc gggcgcaaaa tggcggcaac catggcctcg
    17521 accggcacac ctgcgttttt tgtccaccct gcggaagcgg cacacggcga tttgggcatg
    17581 attgtggaca acgacgtggt cgtcgcgatt tccaattccg gtgaaagcga cgaaatcgcc
    17641 gccatcatcc ccgcgctcaa acgcaaagat atcacgcttg tctgcatcac cgcccgcccc
    17701 gattcaacca tggcgcgcca tgccgacatc cacatcacgg cgtcggtttc caaagaagcc
    17761 tgcccgctgg ggcttgcccc gaccaccagc accaccgccg ttatggcttt gggcgatgcg
    17821 ttggcggttg tcctgctgcg cgcccgcgcg ttcacgcccg acgacttcgc cttgagccac
    17881 cctgccggca gcctcggcaa acgcctactt ttgcgcgttg ccgacattat gcacaaaggc
    17941 ggcggcctgc ctgccgtccg actcggcacg cccttgaaag aagccatcgt cagcatgagt
    18001 gaaaaagggc tgggcatgtt ggcggtaacg gacgggcaag gccgtctgaa aggcgtattc
    18061 accgacggcg atttgcgccg cctgtttcaa gaatgcgaca attttaccgg tctttcgata
    18121 gacgaagtca tgcatacgca tcctaaaacc atctccgccg aacgtctcgc caccgaagcc
    18181 ctgaaagtca tgcaggcaaa ccatgtgaac gggcttctgg ttaccgatgc agatggcgtg
    18241 ctgatcggcg cgctgaatat gcacgacctt ttggcggcgc gg
//
LOCUS       DAWKOG010000024        17780 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.024, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000024 DAWKOG010000000
VERSION     DAWKOG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17780
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.024"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(10..1119)
                     /gene="mltB"
                     /locus_tag="WJK66_000640"
     CDS             complement(10..1119)
                     /gene="mltB"
                     /locus_tag="WJK66_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEZ7578388.1"
                     /translation="MKNRKILPLAICLAALSACTAMEARPPRANEAQAPRADEMKKES
                     RPAFDAAAVFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKAD
                     IVKIMHRPSTSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAV
                     IGIETNYGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGS
                     YAGAMGMPQFMPSSYRKWAVDYDGDGHRDIWGNVGDVAASIANYMKQHGWRTGGKILV
                     SATLAPGADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEY
                     YLGLNNFYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(1028..1279)
                     /locus_tag="WJK66_000641"
     CDS             complement(1028..1279)
                     /locus_tag="WJK66_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578389.1"
                     /translation="MPSDGIFGGGIRISGGARGNVRHSAKPPIAADAAFRAAPLCRFP
                     TYPICSEHIYEKQKNTAAGNLFGGFVCLYGDGGTPAPGK"
     gene            complement(1321..2748)
                     /locus_tag="WJK66_000642"
     CDS             complement(1321..2748)
                     /locus_tag="WJK66_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ7578390.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKYGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQVAQGLEMIFKGEGGGLGVTEPETSGAAIAREMQSYYEYIDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYTLENLERYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKIFIGKIIARLFVFVQ
                     AEHEDTAAFLLNDIRKDILDCRYCG"
     gene            2933..7045
                     /gene="mfd"
                     /locus_tag="WJK66_000643"
     CDS             2933..7045
                     /gene="mfd"
                     /locus_tag="WJK66_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ7578391.1"
                     /translation="MTYPIPKPREKSRWPNLSQGSLPLALARYLPHKQLKVVLTQDAE
                     QALRLQTAWLFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKSDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTDTQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAP
                     FVSLGDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQHLYLSADIFAGRLKNYGQVLP
                     DVSGKEHILPDLAVNRQSEEPLQALKDFQTAFEGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQTTSASEGEGDAVTDQTE
                     FSASATNPLPSPLPQEREQSAAVVSDSLKAAAVSTESRLPPGKSNLHGQIQQQPTPSP
                     VGEGWGEGKAVAAQTEFSATATNPLPNPLPHEREQSAAAVSDDLKAKSSLHLVASNLH
                     RQIRQQPTPSPVGEGWGEGKAVAAQTEFSASATNPLPSPLPQEREQSAAAVSDDLKAK
                     SSLHLVASNLHGQIRQQPAPSPVGEGWGEGKAVADQSAIAVITESDLYQYVARSRIHN
                     RRKKHAAVSDGLLRDLAEINIGDPVVHEEHGIGRYMGLITMNLGGETNEMMLLEYAGE
                     AQLYVPVSQLHLISRYSGQAHENVALHKLGSGAWNKAKRKAAEKARDTAAELLNLYAQ
                     RAAQSGHKFEINELDYQAFADGFGYEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGF
                     GKTEVALRAAFVAVMGGKQVAVLAPTTLLVEQHAQNFADRFADFPVKVASLSRFNNSK
                     ATKAALEGMADGTVDIVIGTHKLVQDDIKFKNLGLVIIDEEHRFGVRQKEQLKRLRAN
                     VDILTMTATPIPRTLSMALEGLRDFSLITTAPSRRLSVKTFVKPFSEGSVREAVLREL
                     KRGGQVFFLHNEVDTIENMRERLETLLPEARIGVAHGQLRERELEQVMRDFLQQRFNV
                     LLCSTIIETGIDIPNANTIIINRADKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITK
                     DAEKRLDAIAAADELGAGFTLAMQDLEIRGAGEILGEGQSGEMIQVGFTLYTEMLKQA
                     VRDLKKGRQPDLDAPLGITTEIKLHSPALLPESYCPDIHERLVLYKRLAVCETVQQIN
                     AIHEELVDRFGLPEQPVKTLIESHHLRLMAKELGIDAIDAAGEAVTVTFGKNNNVDPT
                     EIILLIQNDKKYRLAGADKLRFTAEMENIEVRINTVKNVLKTLQNRCLPK"
     gene            7194..7577
                     /locus_tag="WJK66_000644"
     CDS             7194..7577
                     /locus_tag="WJK66_000644"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEZ7578392.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTIAGKRGSGVICLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            7599..8441
                     /gene="kdsA"
                     /locus_tag="WJK66_000645"
     CDS             7599..8441
                     /gene="kdsA"
                     /locus_tag="WJK66_000645"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEZ7578393.1"
                     /translation="MDIKINGITVGNDAPFVLFGGINVLEDLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVVAMAKTGNVVNIKKPQFLSPSQMKNIVEKFHEAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            8456..8896
                     /locus_tag="WJK66_000646"
     CDS             8456..8896
                     /locus_tag="WJK66_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEZ7578394.1"
                     /translation="MMQTFRKISLYAATLWLGMQIMAGYIAAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGAVLAAAFAALTRRQTALLLFLLSALAANQFLVTPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSIVFMAAALLSIYLSWRLSGKDAV"
     gene            8944..10230
                     /gene="eno"
                     /locus_tag="WJK66_000647"
     CDS             8944..10230
                     /gene="eno"
                     /locus_tag="WJK66_000647"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEZ7578395.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLEGLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAADYPSKAAFYQLGK"
     gene            10244..10522
                     /gene="ftsB"
                     /locus_tag="WJK66_000648"
     CDS             10244..10522
                     /gene="ftsB"
                     /locus_tag="WJK66_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEZ7578396.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(10563..10853)
                     /locus_tag="WJK66_000649"
     CDS             complement(10563..10853)
                     /locus_tag="WJK66_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S ferredoxin-like protein"
                     /protein_id="HEZ7578397.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLSGEDEGLSDSLLK"
     gene            complement(11051..12184)
                     /gene="nrdB"
                     /locus_tag="WJK66_000650"
     CDS             complement(11051..12184)
                     /gene="nrdB"
                     /locus_tag="WJK66_000650"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEZ7578398.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPITVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            complement(12245..13432)
                     /locus_tag="WJK66_000651"
     CDS             complement(12245..13432)
                     /locus_tag="WJK66_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction enzyme"
                     /protein_id="HEZ7578399.1"
                     /translation="MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRPNDVGT
                     TGSHQSGFYIQKHFSDDLFDVVCQKGTNKTISIKINWQDGSVTNSNFKYYGQGTRNEA
                     RITGFGKNFEFLSDKYSGSLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSL
                     YLPKKQEVEDNLTKLFSLFPNFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELF
                     SIFEKREFEIIKSKITDLDSFINFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQ
                     AKTEGKKKPDFLFPGSEEYHAMDESGNFIFQTEKLTMLGSKTTCKDRWRQVLNEADRI
                     PHKHLFTLQEGISDTQIQEMSDENLTLVVPKESVKTFGTFGKTHVLTLENFIKYIKSQ
                     QVS"
     gene            complement(13425..14438)
                     /gene="dcm"
                     /locus_tag="WJK66_000652"
     CDS             complement(13425..14438)
                     /gene="dcm"
                     /locus_tag="WJK66_000652"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ7578400.1"
                     /translation="MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCV
                     FSSEWDDKARQTYQVNFNDIPYGDITLKETKAAIPSKFDVLTAGFPCQPFSIAGVSKK
                     KSLGRETGFLDKAQGTLFFDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLE
                     ELDYQIFYQVMNAKYYVPQNRERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILED
                     DVDNSFTLSDNLWLYLQNYAKKHKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIP
                     QKGKNPRKLTPRECSRLMGFPKDFVIDAVSKTAAYKQFGNSIAVPLVQAIAKQIINEL
                     KNE"
     gene            complement(14496..16775)
                     /gene="nrdA"
                     /locus_tag="WJK66_000653"
     CDS             complement(14496..16775)
                     /gene="nrdA"
                     /locus_tag="WJK66_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEZ7578401.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAADGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAAKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKETRLDYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPDIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCNEPLHYDWESLRADIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQAISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            17304..17615
                     /locus_tag="WJK66_000654"
     CDS             17304..17615
                     /locus_tag="WJK66_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HEZ7578402.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRDTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRSLPYKEQKTQCRAAYEAFDDFDGSRFR
                     R"
BASE COUNT         4460 a         4732 c         4239 g         4349 t
ORIGIN      
        1 taaaaaacat tacaatcccg ggccgccaag cgaattggca atgtccctga ccgccgttac
       61 atacatccga ctgtgattgt actgccatac cgtataaaaa ttgttcaagc ccaaataata
      121 ttcaaacacg ccgggtgcgg tttccagttt gaacaaaacc gccttttcat catcggcaag
      181 ctcttcgccg gggatgatgc cgtacgcctt caaatccgcc accgtccgcg tcagggcggt
      241 tttttcgcca atgattgcct gaacatccgc acccggcgcc aatgttgcag acaccagtat
      301 tttcccgccc gtgcgccaac cgtgctgctt catataattg gcaatcgatg ccgcgacatc
      361 gccaacattg ccccatatgt cccgatgtcc gtccccgtca taatccaccg cccatttccg
      421 gtagctcgaa ggcataaatt gcggcatccc cattgcgccc gcatagctgc ctttaaaggc
      481 gaaaacatcg ccgccttctt cttttgccag ctttaaaagc tcgaccaatt ctttttggaa
      541 aaacccggcg cggcgggggt aatcaaagcc taaggtcgcc aatgcgtccg ccacacggaa
      601 actgcccgta tttttgccgt aattcgtttc aatcccgata accgccacga taagttcggc
      661 aggcacgccg tatttttgcg ccacatcatc gataagcgcg cggttttccg cataaaaccg
      721 gcgcgcgccg cgaaatttcg ccttgcccga atttcccgtg cggaacacat accacggacg
      781 cgatgtggag gggcggtgca taatcttgac gatgtccgcc ttgtaagccg ctttgtcaaa
      841 aaaatcctgc cattccgccc gggaaaaatc ccctttcccg acttcatcgt ccacaaaacg
      901 gcggacattt gcattggcgg caaacccgct gtcggatacc ggtacggctg ccgcgtcaaa
      961 tacggctgcc gcgtcaaacg cggggcggct ttcttttttc atttcatccg cgcggggggc
     1021 ttgggcttca tttgcccggg gcgggcgtgc ctccatcgcc gtacaggcag acaaagccgc
     1081 caaacaaatt gccagcggca gtatttttct gtttttcata aatatgttcc gaacaaatag
     1141 ggtaagtggg aaagcggcac aagggcgcgg cgcgaaatgc ggcatccgcc gcaatcggcg
     1201 gctttgcgga atgccgcacg ttgcctcttg caccgcccga aatccgtatg ccgccgccga
     1261 aaatgccgtc tgaaggcact tcccccttca gacggcattg cctgccgccg tatttgcccg
     1321 ctatccgcaa tatcggcagt ccaatatgtc tttgcggatg tcgttcagca ggaaggctgc
     1381 ggtgtcttcg tgttccgcct gtacgaagac aaagagtcgg gcgatgattt tgccgataaa
     1441 gattttttgc agggcgcagg catcggtcag ggtgtgttcc tgcaggaagc tgcggatgtc
     1501 ttcgggcaaa gtgtagtcgc gggcgacggc ggcaaagcgg gcatcggttt gcaggcggtc
     1561 gagcagggtt tggtttttgt ccaacttcat gcctgcttct ttttcttcgg cggtggcgcg
     1621 gacaaactgg tcgtaaattt cggcataaag catatcgttc gggccttcaa aaatcgtgaa
     1681 ggggcggatg tcgatagcga tattgccggc ggtgtgtccg cgttcaaaac ccttcgcgcc
     1741 caagagtttt tgcaacattt gcgcggcggc gtaagtgtat tccgtggcga gggttttgac
     1801 gatgttcgcc tccatcagtt gatgggcgac gggtgcaacg ggcgaaacgg aatggcagac
     1861 gtagcggtaa agaatctcgg aaacctgatg gcggcgccgg atttcgcggc gttcgtaatc
     1921 gacgaatctg atgtcgttgc ggacgtatcg ttccaggttt tcaagggtgt attccataat
     1981 gccgtgcgtc atgccgatca gttgcaggcg gctgcggata aagatgtttt ggaacgcgcg
     2041 caaaccggca gcgtcgctct gggagagttt catcacggcg gttgcaggca tttcggcatc
     2101 gatgcggttg acggcgtaac ggacggcgcg caagccttcg gatgcgaggg tttcgcagcg
     2161 gatgtatgtt ttggggacga gcagcaggtc gatgactttg gcgagtttgc cgtttttgcg
     2221 ctctttggcg gcaacgagga ggaagtcgct ttgcgagttg ccctgccagt atttcgcggc
     2281 gttgacgtaa atggtttgtc cgtcgatata ttcgtagtag gactgcattt cgcgtgcaat
     2341 cgccgcgccg gaggtttcgg gttcggtaac acccaaaccg ccgccctcgc ctttgaaaat
     2401 catctccaaa ccttgcgcga cttgcgcttc atcgccgaac tcttgcagtg gctgcaacac
     2461 cagcgcgcct tcgatgccgg tacgcagcgt aacgggcacg ccgtaatgcc ccgcaatccg
     2521 caggacttct tggatttcaa actggctgcc cttgcgcccg ccgtattttt tgtcgaggaa
     2581 gggcaacagc aaacccgcct gcttcaaggc aagccatttg tcttcgggca ggtatcgcat
     2641 caggtcgata ccgtctgaaa aaatgcggcg gaatgcggat tcgatgtgct ttaaaaaagc
     2701 agccgtgtcc atagttgacg gctgcgcgct cggttcggtg tgtatcatcg gcttcctctg
     2761 tcggttccca ttaatcggcg gccggtcaaa ccgcctgcca cagtttagag ttgattttct
     2821 aaactttacc acaaagtgcg ccgggcaaca atccgccgac ctttcagacg gcatcgcgtc
     2881 ccctcccgtg ctaaaatgac cgtttgcatc actgtccgcc gattgccgca ctatgaccta
     2941 ccccatcccc aaaccccgtg aaaaatcccg ttggcccaat ctttcgcaag gctcgctgcc
     3001 cttggctttg gcgcgttatc tgccgcataa gcagctcaag gttgtcttga ctcaagatgc
     3061 ggaacaggcg ctacgccttc agacggcatg gctgtttttc cgtccgcacg atacggcggt
     3121 gttcttgccg gactgggaaa cgctgcctta cgagcgtttt tcgccgcatc aggatttagt
     3181 gtcggagcgc ttgtcggcat tgtggcagat taaaagcggc gcggcggacg tgttgttcgt
     3241 gccggttgct acggcgatgc agaagctgcc gcctgtgccg tttttggcgg ggcgcacgtt
     3301 ttggctgaaa acagggcaga ctttggatat aggccgtctg aaaagcgatt tggtggatgc
     3361 aggttataac catgtttccc acgttgttgc ggcaggcgag ttcgctgtgc gcggcggcat
     3421 tgtcgatttg ttccccatgg gcagcgaaat gccctaccgc atcgatttgt ttgacgatga
     3481 aatcgacagc atcaaaacct ttgatacgga cacgcagcgc accatctccc ccgtttccga
     3541 aatccgcctg ctgccggcgc acgaattccc caccgacagc gaggcgcaaa aaatcttccg
     3601 cagccgcttc cgcgaggaag tcgatggtaa tccgaacgat gcggctgtgt acaaagcagt
     3661 aagcaacggc cacttcggtg cgggcgtgga atattatctg ccgctgtttt ttgaaaacga
     3721 gctggaaacg ctgtttgact atatcggcga agatgcgccg tttgtttcat tgggcgacgt
     3781 tcacgccgag gcaaaccgct tttggagcga cgttaaatca cgttacgcca tggcgcaggg
     3841 cgacgaaacc tatccgcctt tgcttccaca gcatttgtat ctctctgccg atatttttgc
     3901 aggccgtctg aaaaactacg gacaagtgct gcccgatgtt tccggcaagg aacacatcct
     3961 gcccgacctt gccgtcaacc gccaatccga agagccgttg caggcattga aggattttca
     4021 gacggcattt gaaggacgga ttttgctgtg cgccgaaagt ttgggacggc gcgaaaccat
     4081 gctcggtttc ttgcagcaaa acggtttgaa agccaaacct gtgtccgact ggcagggctt
     4141 cttgtcggcg catgagccgc tgatgattac agttgcgccg ttggcatacg ggttcaaact
     4201 gggcggactg caatcgccga accaacagca aactacttcc gcctccgaag gagaaggcga
     4261 tgcggttacc gaccaaaccg aattttccgc atccgcaaca aaccctctcc ctagccctct
     4321 cccacaggag agggaacaaa gtgcagccgt cgtttcagac agtctgaaag cagccgccgt
     4381 ttcaaccgaa agccgcctgc cccccggtaa aagtaatctg cacgggcaaa tccaacagca
     4441 acctactccc tcccccgtgg gggagggttg gggagagggc aaagcagttg ccgctcaaac
     4501 tgaattttcc gcaaccgcaa caaaccctct ccctaaccct ctcccacatg agagggaaca
     4561 aagtgcagcc gccgtttcag acgacctgaa agccaaaagc agcctgcatc tcgtcgcaag
     4621 taatctgcac aggcaaatcc gacagcaacc tactccctcc cccgtggggg agggttgggg
     4681 agagggcaaa gcagttgccg ctcaaaccga attttccgca tccgcaacaa accctctccc
     4741 tagccctctc ccacaggaga gggaacaaag tgcagccgcc gtttcagacg acctgaaagc
     4801 caaaagcagc ctgcatctcg tcgcaagtaa tctgcacggg caaatccgac agcaacctgc
     4861 cccctccccc gtgggggagg gttggggaga gggaaaagca gttgccgacc aaagtgccat
     4921 cgccgtcatt accgaatccg acctctacca atacgtcgcc cgttcgcgca tccacaaccg
     4981 ccgcaagaaa cacgccgccg tttcagacgg cctgttgcgc gaccttgccg aaatcaatat
     5041 cggcgatccc gtcgtgcacg aagaacacgg catcgggcgg tatatgggct tgatcacaat
     5101 gaatttgggc ggcgaaacca acgaaatgat gttgctcgaa tacgcaggcg aagcgcagct
     5161 ttatgtgccc gtttcgcaac tgcatttaat cagccgctac tccggccagg cgcatgaaaa
     5221 tgtcgccctg cacaagctcg gcagcggcgc gtggaacaag gcaaaacgca aagccgccga
     5281 aaaagcgcgc gataccgccg ccgagttgct caacctctac gcccaacgcg ccgcccaatc
     5341 gggacacaag tttgaaatca acgagttgga ttatcaggcg tttgccgacg gcttcggcta
     5401 cgaagaaacc gaagaccagg cagccgccat cgccgccgtg attaaagatt tgacgcaggc
     5461 gaagccgatg gaccgcctcg tgtgcggcga tgtcggtttc ggcaaaaccg aagtcgccct
     5521 gcgcgccgcg tttgtggcgg taatgggtgg caaacaagtt gccgtactcg ccccgaccac
     5581 gcttttggtc gagcagcacg cgcaaaactt cgccgaccgt ttcgccgatt tccccgtgaa
     5641 agtcgccagc ctttcgcgtt tcaacaacag caaagccacc aaagccgcgc tggaaggcat
     5701 ggcagacggc acggtcgata ttgttatcgg tacgcacaaa ctggtgcagg acgacatcaa
     5761 attcaaaaac ttaggtttag tgattatcga tgaagaacac cgcttcggcg tgcgccagaa
     5821 agagcagctc aaacgcctgc gcgccaatgt cgacatcctc accatgaccg ccacaccgat
     5881 tccgcgcact ttaagtatgg cgttggaagg actgcgcgac ttctcgctga ttaccaccgc
     5941 gcccagccgc cgcctctccg tcaaaacctt tgtcaaaccc tttagcgaag gcagcgtgcg
     6001 cgaagccgta ttgcgcgaac tcaaacgcgg aggacaggta tttttcctgc acaatgaagt
     6061 ggataccatc gaaaacatgc gtgagcggct ggaaaccctg ctgcccgaag cccgcatcgg
     6121 cgtggcgcac ggacaactgc gcgagcgcga gctggaacaa gtcatgcgcg actttttgca
     6181 gcaacgattt aacgtgttgc tctgttccac catcatcgaa accggtatcg acattcccaa
     6241 cgccaacacc atcatcatca accgtgccga caaattcgga ttggcgcagc tgcaccagct
     6301 tcgcgggcgc gtcggacgca gccaccacca agcctacgcc tacctgctca cgcccgaata
     6361 catcaccaaa gacgcagaaa aacgcctcga cgccatcgcg gcagcagacg aactcggcgc
     6421 aggtttcacc ctcgccatgc aggatttgga aatccgcggc gcaggcgaaa tcctcggcga
     6481 agggcagtcc ggcgaaatga tacaggtcgg cttcacgctc tacaccgaaa tgctcaaaca
     6541 agccgtgcgc gatctcaaaa aaggccgcca acccgacctc gacgcgccgc tgggcatcac
     6601 caccgaaatc aaactgcaca gccccgccct actgcccgaa agctactgcc ccgacatcca
     6661 cgagcgtctc gtcctctaca aacgcctcgc cgtctgcgaa accgtgcagc aaatcaacgc
     6721 catacacgaa gaactcgtcg accgcttcgg cctgcccgaa caacctgtca aaacccttat
     6781 cgaaagccac cacctgcggc ttatggcaaa agaattgggt atcgatgcca ttgatgcggc
     6841 cggcgaagcg gtaacggtaa ccttcggtaa aaacaataat gtcgatccaa ccgaaatcat
     6901 cctgctgatt cagaacgaca aaaaataccg ccttgccggc gccgataagc tgcggtttac
     6961 cgcagagatg gaaaatatcg aggtcagaat caacaccgta aaaaacgttt taaaaacctt
     7021 gcaaaacaga tgcctgccca aataaagccg acaccgcaat gccgtctgaa acaccgtttt
     7081 ccttgtccga aagccgccat tatgaatttg aaggaaactc cactataata cggcattcag
     7141 atttccagac ggcatcgcgc ccgtcaaacc gcacacaaac caaaaggaaa tacatgttcc
     7201 gtactatgct tggcggaaaa atccaccgcg ccaccgttac cgaagccgat ttaaactatg
     7261 tcggcagtat taccgtcgat caagatctgt tagacgcggc aggcatctac cccaacgaaa
     7321 aagtcgccat cgtcaacaac aacaacggcg aacgttttga aacctatacc attgcaggga
     7381 aacgcggcag cggcgtgatt tgtctgaacg gtgctgcggc aaggctggtg cagaaaggcg
     7441 atatcgtcat catcatgtct tacgtccaac tctccgaacc cgaaatcgcc gcacacgaac
     7501 ccaaagtcgt cttggtagac ggaaacaaca aaatccgcga catcatctcc tatgagccgc
     7561 cgcacaccgt gctgtaaccc tgaaaacgga cattgaccat ggatattaaa atcaacggca
     7621 ttaccgtcgg taacgacgct ccttttgtac tgttcggcgg catcaacgtt ttagaagatt
     7681 tggattccac cctccaaacc tgtgcgcatt acgtcgaagt tacccgaaaa ctcggtattc
     7741 cctatatctt taaagcctct ttcgacaagg caaaccgttc ctccatccat tcttatcgcg
     7801 gcgtaggctt ggaagaaggc ttaaagattt ttgaaaaagt caaagcagag ttcggcatcc
     7861 ccgtcattac cgacgtacac gaaccccatc agtgccaacc cgtcgccgaa gtgtgcgatg
     7921 tcatccagct tcccgcattc cttgcgcggc agaccgattt agtggttgcc atggcaaaaa
     7981 ccggcaacgt cgtcaacatc aaaaaacctc agttcctcag cccctctcaa atgaaaaaca
     8041 ttgtggaaaa attccacgaa gccggcaacg ggaaactgat tttatgcgaa cgcggcagca
     8101 gcttcggcta cgacaacctc gttgtcgata tgctcggttt cggcgtgatg aaacagactt
     8161 gcggcaacct gccggttatt ttcgacgtta cccattccct gcaaacccgc gatgccggtt
     8221 ctgccgcatc cggcggtcgt cgcgcacagg ctttggattt ggcacttgca ggcatggcaa
     8281 cccgccttgc cggcctgttc ctcgaatcgc accccgatcc gaaactggca aaatgcgacg
     8341 gccccagcgc gctgccgctg caccttttag aagatttttt aatccgcatc aaagcattgg
     8401 acgatttaat caaatcacaa ccgattttaa caatcgagta acacggtttc gccttatgat
     8461 gcagactttc cgaaaaatca gcctgtatgc cgcaaccttg tggctcggta tgcagattat
     8521 ggcgggttat atcgccgcgc cggtgctgtt caaaatgctg cccaaaatgc aggcgggcga
     8581 aattgccggc gtattgttcg acatcctctc ttggagcggg cttgccgttt ggggcgcggt
     8641 actggctgcc gcctttgccg ccctaacccg gcggcaaacc gccctgctgc tttttttatt
     8701 gtccgccctt gccgccaacc aatttttggt tacccccgtt atcgaggcac tgaaatacgg
     8761 gcatgaaaat tggctgttgt cgtttgtagg cggatccttc ggaatgtggc acggtatttc
     8821 cagtattgtt tttatggcgg ccgccctact ttcgatttac ttaagttggc ggctttccgg
     8881 caaagatgcc gtctgaagcc ctcccatttt tttacctccc ttcacttcac ttggagaaca
     8941 ttcatgagcg caatcgttga tattttcgcc cgcgaaattt tggactcacg cggcaacccc
     9001 acagtcgagt gtgatgtatt gctcgaatcc ggcgtaatgg gacgcgcagc cgtaccgagc
     9061 ggcgcgtcca ccggtcaaaa agaggctttg gaacttcgcg acggcgacaa atcccgttat
     9121 tcgggcaagg gcgtattgaa ggcggtcgaa cacgtcaaca accaaatcgc ccaagccctc
     9181 attggtatcg atgccaacga gcaatcttat atcgaccaaa tcatgatcga attggacggt
     9241 actgaaaaca aaggcaattt gggtgcgaat gcgactttgg cggtttctat ggcggttgca
     9301 cgcgccgctg ccgaagactc aggcctgccg ctttaccgct acttgggcgg cgcaggcccg
     9361 atgtccctgc ccgtaccgat gatgaacgtc atcaacggcg gcgaacacgc caacaacagc
     9421 ctgaacatcc aagagtttat gattatgccc gtcggcgcaa aatctttccg cgaagcgttg
     9481 cgctgcggtg cggaaatttt ccacgccttg aaaaaactgt gcgacagcaa aggcttcccg
     9541 accacagtcg gcgacgaagg cggtttcgcc cccaacctga acagccacaa agaagccctg
     9601 caactgatgg tcgaggcgac cgaagccgcc ggctacaaag cgggcgaaga cgtattattc
     9661 gcattggact gcgcgtccag cgagttctac aaagacggca aataccactt ggaagccgaa
     9721 ggccgctcct acaccaacgc ggaatttgcc gaatatctgg aaggcctggt caacgagttc
     9781 cccatcatct ccatcgaaga cgggatggat gaaaacgact gggaaggctg gaaactgctg
     9841 accgaaaaac tgggcggcaa agtccaactc gttggcgacg acctcttcgt taccaacccg
     9901 aaaatccttg ccgaaggcat tgaaaaaggc gtggcaaacg cactattggt caaagtcaac
     9961 caaatcggta ctttgagtga aaccctgaaa gccgtcgact tagccaaacg caaccgctac
    10021 gccagcgtaa tgagccaccg ctccggcgaa accgaagaca gcaccattgc cgacttggca
    10081 gtcgccacca actgtatgca gatcaaaacc ggttctttga gccgttccga ccgcatggcg
    10141 aaatacaacc aactgctgcg tatcgaggaa gaattggcgg aagccgccga ctaccccagc
    10201 aaagccgcat tctaccaact gggcaaataa aaaaggttaa ggtatgaagt gggtaactgt
    10261 cgttttatcc ttcgcacttg tctgttgcca atacagcctc tggttcggca aaggcagcat
    10321 cggacgcaac agcagtctga gagaacagat tgccgttcaa gaagaaaaaa accagacact
    10381 cgccctacgc aatcattccc ttgccgccga agtctatgat ttggaaaacg gtcaagaagc
    10441 catttcggaa atcgcccggg tagaactggg ttatatccaa gacggtgaaa ccttttaccg
    10501 actcatcagg cataaccggt aataccgtca aaaagccgtc cgaaccaatg ttcggacggc
    10561 ttttatttca acaaactgtc agacagcccc tcatcctccc ccgacaaacc gcaatccagc
    10621 ctgacatccc cctcgacgca acagcagcac ggcagtatct cgtcccgccc caaaaaagcc
    10681 aaaggcggct cccgataagt aacgcttccc tccaaaatct tcactcggca cgatccgcaa
    10741 tatccgcttc ggcactgata ttccaccata tgccccgtcc gttccaagcc ttccaacagg
    10801 gtctcgccct ccaagagctc aaaaaaaccc ttattcgtac caatgcgcgc catttccgac
    10861 caatcaaaaa tatagtggat taaatttaaa ccagtacggc gttgcctcgc cttgtccgga
    10921 tttttgttaa tccactataa tccactataa aaggaacaat aaccgatcct acccgctgtt
    10981 tttcccatca tacaacatac aaatgccgtc tgaaacatcc ggcttcagac ggcatttttt
    11041 caaaaaacgt ttacaactca aaatcgccca aatcatccgt attcacttca gaatcgatct
    11101 gaccgatcaa ataagaggat atttccactt cctgcggcgc gacctgtacg ttatcggacg
    11161 acagccacgc attaatccac ggaatcgggt tttgatttgc gccttcaaat ccggccggca
    11221 accccaccgc ctgcatacgc agattggtaa tatattcgac gtactgggat aagatttctt
    11281 tgttcaaacc aatcatcgaa ccgtctttaa acaaatatgc cgcccattct ttttcctgtt
    11341 ccgccgcttt tttgaagagt tggaaacatt cgtcctgcaa ctcagcggca atttctgcca
    11401 tttcagaatc atcgacaccc gaacgcatca gattaagcat atgctgcgtg ccggtcaggt
    11461 gcagggcttc gtcgcgggca atcagtttga tgattttggc gttgccttcc atcaactcgc
    11521 gctcggcaaa agcaaacgag caggcgaatg aaacgtagaa acggatggct tccaacacgt
    11581 tgacgcacat caggcagaga tagagttttt tcttcaaccc gcgcaaagac acagtaatgg
    11641 gttttccgcc gacattgtgc accccttcgc ccaacagatt gtaatactgg gtgtattcga
    11701 ttaagtcgtc gtaatagcag gcaatgtctt cggcgcgggc ggtaatgtat tcgttttcga
    11761 caatatcatc aaacacgacc gacggatcat tcacaatatt gcggatgatg tgggtatagc
    11821 tgcgcgagtg tatggtttcg ctgaagctcc acgtttcaat ccacgtttcc aactcgggaa
    11881 tcgaaaccaa aggcagcaag gcaacattcg gactgcgccc ttggatggaa tcgagcagtg
    11941 tttggtattt cagattgctg atgaaaatat gtttttcgtg ttctggcagg ttggcgtagt
    12001 cgatacggtc gcgcgacacg tcgatttctt ccggccgcca aaaaaacgac aattgttttt
    12061 caatcagttt ttcaaatacc tcgtatttct gctggtcata acgggcgaca ttaaccggct
    12121 gaccgaaaaa catcggctca ttcagcgcgt cgtttttggt tttgggaaag gtgctgtatg
    12181 acatagttaa gtgttcgcag gacataatct attctctata ttaattaaaa atgatctgta
    12241 agttttaact aacctgctga gactttatat atttaataaa attttctaaa gttaaaacat
    12301 gggttttccc aaaagttcca aatgttttta cggattcttt agggacaact aaggttaaat
    12361 tctcatcact catttcctga atttgagtat cagatatacc ttcttgtaga gtaaataaat
    12421 gcttatgagg aattcgatct gcttcattta aaacttggcg ccagcgatcc ttacaagtag
    12481 tctttgaacc tagcattgtc agcttttcag tttgaaatat gaaattccct gattcatcca
    12541 tcgcatggta ctcttcactt ccaggaaata aaaaatctgg tttctttttc ccttctgtct
    12601 tcgcttgcgt ctcaaatttt aaggaaaatt cgtcaaatat tcttgataaa tgtaactcta
    12661 aagattttcc tgctcttgct tttctgcgat ttgtaaaaga atgtgcaaaa ttaatgaagc
    12721 tatccaaatc agtaattttt gatttaataa tttcaaattc acgtttttca aaaatggaaa
    12781 ataattcata ttcctttgaa acccattgtt ttaacacatt actaacgtta gttttattta
    12841 atgcaatatt ttcccttgcc aaaactgcca tttcttcagt tttggggaag ttaggaaata
    12901 gtgaaaataa ttttgttagg ttatcctcta cttcttgttt tttaggtaaa tataaacttc
    12961 ctggtaaaat gttagtttct gcgataaata tttcaatatc ttcatctgaa gataaaacaa
    13021 aagcatggaa taataaatct ttataacaag ctctgcacaa taccaataag gaaccactat
    13081 atttatcact taaaaattca aaattcttgc caaaacccgt aattctcgcc tcatttctcg
    13141 tgccttggcc gtaatattta aaattgctat tagtgacgct tccatcttgc caattaattt
    13201 tgattgaaat agttttattt gttccctttt ggcaaactac atcaaagagg tcgtctgaaa
    13261 aatgtttttg aatataaaat cctgattggt gactaccagt agtaccaaca tcattgggac
    13321 gaatataacg acagtatact gcaattgatt tatttgctgt ctgtattgct gaagctacta
    13381 agtcatcgga tttatttact acttgtattg tctgagctgc caagtcattc attttttaac
    13441 tcatttataa tttgtttagc aatagcttga accaacggta cagcaattga attgccaaac
    13501 tgcttgtatg cagctgtctt ggatactgca tcaataacaa aatctttagg aaatcccatt
    13561 aaacgcgagc actccctagg tgtcagcttc ctaggatttt ttcctttctg agggatgagt
    13621 atttcggaac catctttgta atatcgtgca gatagagttc gtgatattcc atctaaatca
    13681 actaatccaa aaccaaatcc attacccttt gccttatgtt ttttagcgta attttgaagg
    13741 taaagccata agttatcaga aagagtaaaa gaattatcta catcatcttc caaaatttgc
    13801 tttaattttg gttgtgattc tggtggggaa ggaaaattga aatttatttc cttattaaaa
    13861 tattgtctat caaaacctac aataaaaata cgctccctat tttgaggaac ataatatttt
    13921 gcattcataa cttgataaaa tatctgatag tcaagctctt ctaaagtccc tttaattact
    13981 ttaaatgtat ttcctttgtc atgcgaaaca aggtttttca cattctctaa aagaaaaatt
    14041 ttaggtcgat gttttccaat aatttcagca acatcaaaaa atagagttcc ctgcgcctta
    14101 tctaagaagc ctgtttctcg tcctaggctt tttttctttg aaacaccagc tatagagaat
    14161 ggctgacacg ggaatcctgc tgttaataca tcaaacttac ttggaatagc tgctttggtt
    14221 tcctttaatg taatatctcc ataaggaata tcattaaaat ttacttggta ggtttgacgg
    14281 gctttatcat cccattcact agaaaataca catcgcccac caacattctc cattgcaatg
    14341 cgaaaaccac ctattcccgc aaataaatca ataaaagtaa atttctcatt atttaaggta
    14401 actatattat ctaattgatt ttgtatttta tttttcatat tttatatttc agatgattta
    14461 ttaatctaaa ggccatctga aaactccccc tttcatcaaa tcttacaagc cccgcccgcg
    14521 cagccgtcat cctgaatatc ggtctgcgta tcgtccgcgc cgtcgcgggt gttatggtag
    14581 tacagggttt tgacgccgta tttgtaggcg gtcagcaggt ctttgagcat ttgtttcata
    14641 gaaactttgc cgccttcgaa tttgcccggg tcgtaggcgg tgttggcgga aatcgcctga
    14701 tcgacgaatt tttgcatcac gccaacaagt ttcagatagc cttcgttgcc gggaagctgc
    14761 cacagggttt cataggcatt tttcagggtt tcaaactccg gcacgacttg tttcaaaatg
    14821 ccgtctttcg atgctttgac cgttaccaat ccgcgcggcg gctcgatacc gttggtggcg
    14881 ttggcgattt gcgaactggt ttcagacggc atgagtgcgg ttagagtaga gttacgcagg
    14941 ccgtatttga cgatgtcggc acgcaggctt tcccaatcgt agtgcaaagg ctcgttacac
    15001 acagcgtcca agtctttttt gtaggtgtcg atgggcagtt tgccttgcga ataaacggtt
    15061 tggttaaaga gcgtgcacgc accgtattct ttggcaaggt ttgccgatgc tttgagcagg
    15121 taatactgta tggcttcaaa ggtacggtgg gtcagaccga gcgcggagcc gtcgctgtag
    15181 cggacaccgt ttttcgccaa ataatacgca tagttgatca cgccgatacc gagcgaacgg
    15241 cggcccatag tggaggtacg cgcggcttct accggatatc cctgataatc taaaagtgca
    15301 tcgagcgcgc gcacggtcaa atcggcaagc ccttccaatt cgtccaagct gtttaatgcc
    15361 cccaagttga aggcagacag ggtacacagg gcgatttcgc cgttcgggtc gttgatattg
    15421 tccaaaggtt tggtcggcag cgcgatttcc atacacaagt tggactgatg aacaggcgca
    15481 acgcgcggat cgaacgggct gtgcgtattg cagtgatcga cgttttgaat gtagatgcgc
    15541 ccggttccgg cacgctcctg catcagcgtg gaaaacaggt cggcagccgg aatgatgcgc
    15601 ttgcggatat cagggtcttg ctcgtatttc gtatagagcc gctcaaattc gtcttggtcg
    15661 gcaaaaaacg cttcgtacaa tcccggaacc tcgttgggcg aaaacagcgt aatgttgccg
    15721 cccttaatca ggcgggtgta cagcaggcgg ttgatttgca cgccgtaatc aagctgacgg
    15781 atacggttgt cttccacgcc ccggttgttt ttcaacacca gcaggctttc ggcttcgata
    15841 tgccacaagg ggtagaacaa ggttgccgcg ccgccgcgca cgccaccttg cgaacaggat
    15901 ttgactgccg cctgaaacat tttaaagaag ggaatgcagc cggtatgccg tgcttcgccg
    15961 ccccggattt cgctgtccag tccgcggata cgtccggcat tgatgccgat gcccgcacgc
    16021 tgggaaacgt atttcacaat cgcgctggtg gtggcgttga tggaatccag gctgtcgtcg
    16081 cattcgatca atacgcagct tgagaactgg cgcgtaggcg tacgcacgcc gctcataatc
    16141 ggggtcggca gcgatacttt aaatgtggaa acggcatcgt aaaaccgttt gacgtaatcg
    16201 aggcgtgttt ctttgggata tttgctgaaa agacacatcg ccaccagaac atataagaac
    16261 tgcggtgttt cataaatctg gcgggtaacg cggttctgta ccagatattt gccttcgagc
    16321 tgtttgacag cggcatagga aaaggacata tcgcgttcgt ggtcgatata ggcgttcagc
    16381 tcgtcaaatt cttcgcggct gtaatcctca aggatatgcc tgtcgtattt tgcggcatcg
    16441 gtaagttttt taacgtggtc gtaaaggtgc ggcggctcgt actcgccgta ggctatttta
    16501 cgcagatgga aaatcgccaa acgcgcggca aggtattggt agtccggggt atcttccgaa
    16561 attaaatcgg cagcggcttt gatgatggtt tcgtggatgt cgtcggtgcg gatgccgttg
    16621 tagaactgga tgtgcgattt caactcgacc tgagatacgg aaacattttc caatccgtcc
    16681 gccgcccaag tgacgacacg gtgaatctta tccaaatcaa tggcttctaa tcttccgtct
    16741 cgtttggtta ctttcaaatc agtcggtgta ttcatcgctt cctcttccac tcttgatatt
    16801 caagacacag tcttttcaaa taaattaagg cagacaatat agtggatttt tggcattttc
    16861 tccagtcttg acaacggttg tatttttcag tttcaggcaa tgcgcgataa aacccgcctg
    16921 tcgtattttt cctataatat ttgttttatc tgataattct ttaccgataa aaaacgggta
    16981 aattttttgc cttttgaccg gctccggcta caaggcggcg aaataaggat tttccgacga
    17041 aaaaggaaag cttcctgttt tctgcccctg caaattgtta aattttgcaa agtatgattt
    17101 tgccgcgccg ccgccgacaa attccatttt cttaccgatt ggaatttatt attgagatta
    17161 atgtgttatt tgaatccgca tatcaaacgg caagttttgt cggctgaatg agtctgaaac
    17221 tgccgacaat ttgcccgttc catccttcca tcctatactg gaaggaatga caaactgaaa
    17281 ggatcgtcat atctgtcaaa aagatgcttg ccatactgtt gtctgcaata ttgggactgg
    17341 tatcgacaac tgccgctgcc ggtacgtcag aacccgccca ccgcgatacc aaacatatcc
    17401 gcaaggcaaa caagcagatg ctgcaccccg aatgcaggaa atatttggaa cgccgtgccg
    17461 cgtggtaccg atcgcaaggc aacgtgcagg aattgcgcga aaacaaaaag gcgcgcaaag
    17521 cattccgctc cctgccttat aaggaacaga aaacccaatg ccgggcggct tatgaggctt
    17581 tcgatgattt cgacggcagc aggttccgcc gttaatccca tataaaatat gccgtctgaa
    17641 cacaggttca gacggcatat tttatgaata caacaaacta tccttacgcc atttcgtttg
    17701 aggtcctccc gttgccgcag ccttaatata gtggattaac aaaaatcagg acaaggcgac
    17761 gaagccgcag acagtacaga
//
LOCUS       DAWKOG010000025        17158 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.025, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000025 DAWKOG010000000
VERSION     DAWKOG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17158
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.025"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(49..1218)
                     /locus_tag="WJK66_000655"
     CDS             complement(49..1218)
                     /locus_tag="WJK66_000655"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEZ7578403.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSESGKVKRIDTVVLSTQHDPEIGYEELKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1401..1511)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1564..2460
                     /locus_tag="WJK66_000656"
     CDS             1564..2460
                     /locus_tag="WJK66_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEZ7578404.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGMNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMF
                     KAVHGWEHVQQALDKHEGLLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     AEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2504..3568)
                     /gene="tsaD"
                     /locus_tag="WJK66_000657"
     CDS             complement(2504..3568)
                     /gene="tsaD"
                     /locus_tag="WJK66_000657"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEZ7578405.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     DKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVRAENGADDIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGSMTVQIPTPTGKPKHPSEKVSVFFPPTAYCTDNGAMIAFAGAMHLKT
                     GREAGAFNVRPRWPLSEIVK"
     gene            complement(3685..4872)
                     /gene="ccsB"
                     /locus_tag="WJK66_000658"
     CDS             complement(3685..4872)
                     /gene="ccsB"
                     /locus_tag="WJK66_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEZ7578406.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEGKGGKLWLPPSALIDEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(4865..6817)
                     /locus_tag="WJK66_000659"
     CDS             complement(4865..6817)
                     /locus_tag="WJK66_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HEZ7578407.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTVSREGGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILSASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKGAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7094..7717)
                     /locus_tag="WJK66_000660"
     CDS             complement(7094..7717)
                     /locus_tag="WJK66_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HEZ7578408.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPMVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            7923..8552
                     /locus_tag="WJK66_000661"
     CDS             7923..8552
                     /locus_tag="WJK66_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEZ7578409.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPEEN"
     gene            complement(8673..11069)
                     /locus_tag="WJK66_000662"
     CDS             complement(8673..11069)
                     /locus_tag="WJK66_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEZ7578410.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRREKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11221..12336
                     /locus_tag="WJK66_000663"
     CDS             11221..12336
                     /locus_tag="WJK66_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEZ7578411.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQHTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12339..12938
                     /locus_tag="WJK66_000664"
     CDS             12339..12938
                     /locus_tag="WJK66_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEZ7578412.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            12939..13586
                     /locus_tag="WJK66_000665"
     CDS             12939..13586
                     /locus_tag="WJK66_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEZ7578413.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13604..14149
                     /locus_tag="WJK66_000666"
     CDS             13604..14149
                     /locus_tag="WJK66_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEZ7578414.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14157..16442
                     /locus_tag="WJK66_000667"
     CDS             14157..16442
                     /locus_tag="WJK66_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEZ7578415.1"
                     /translation="MTPLLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSEPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQAAAPAKQQAAAPAKQT
                     NIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKT
                     PVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKT
                     FTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQ
                     ARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKYKNVEEFRS
                     ILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQV
                     MIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKWGAETKINL
                     PITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIP
                     FTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKITKDSPAQCASGNQTIL
                     CISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFKTRGKKTDR
                     RELLIFITPRIMGTAGNSLRY"
BASE COUNT         4605 a         4863 c         3917 g         3773 t
ORIGIN      
        1 aatgccgtct gaactgttca gacggcattt ttgattttca aaccggaatt acagccccgc
       61 tgccgccctc aatgcagcag ctttgtcggt gcgctcccaa gtgaactcag gttcttcgcg
      121 gccgaaatgt ccgtaagcgg cggatttact gtaaatcggg cgcaagagat cgagcatttg
      181 gacgatgcct ttggggcgca ggtcgaaatg ttcgcgaact aaggtaatca gtttttcctc
      241 gctgattttg cctgtgccga aagtgtcgat ggaaatcgaa gtcggttcgg caacgccgat
      301 ggcgtaggaa acttggattt ggcattgggt tgccaaaccg gcggcgacga tgtttttcgc
      361 aacgtagcgg caggcgtaag cggcggaacg atccactttg gacgggtctt tgccggagaa
      421 cgcgccgccg ccgtgcggag ccgcgccgcc gtaggtatca acgatgattt tacggccggt
      481 caaaccgcag tcgccttgcg ggccgccgat gacgaagcgg ccggtcgggt tgatcaggta
      541 tttggtttcg tcggtcagca gttcagacgg caggacgggc ttgataatct gctcaatgac
      601 ggcgtttttc aactcttcat aaccgatttc cgggtcgtgt tgcgtagaga ggacgacggt
      661 atcaatgcgt tttactttgc cggattcgct atcgtaaacc acggtcagtt gggctttggc
      721 atcaggacgc agccacggca ggcggccgtc tttgcgcaat tcgctttggc gttgcatcag
      781 gcggtggctg taatagatgg caaacggcat cagggtcggg gtttcgtcgc aggcgtagcc
      841 gaacatcaga ccttggtcgc ctgcgccttg gttcaagtcg ataccttcgc cttcgtttac
      901 gccttgggcg atgtcggggg attgttggtc gtagtacacg ccgaccgcgc agccgttggc
      961 atcaaagccc agctcggagg agttgtagcc gatgcgtttg atggtttcgc gtgcgacttt
     1021 gatgtagtcc acttgggcgg tggtggtgat ttcgcctgcc aatacgcaca agcctgtgtt
     1081 gaccaaagtt tctgcggcga cacgcgcttt ggggtcttgc gccaagatgg catccaaaat
     1141 cgcgtcggat acttggtcgg caactttgtc cggatggcct tcggataccg attcggaagt
     1201 aaacagatat tcgctcattg agattttcct tgaaaaacaa accatcttct tcagacggca
     1261 tgttgtatga acataatgtc gacagcggga aatatagcaa aatttcccta ttcataccat
     1321 tcagttgaga aatattccca tttgaatggc actttggaat ctctgcccgt acgtttctta
     1381 caggcaaaaa aattcccgca tcaagcgggt ttggattgct ctggtgagcc acatcggctt
     1441 ttcaaccgtc caccttactt ttccttttga aaagcaggtt ggcatggaat tcccaactct
     1501 taatgcagcg cggatggtag cagaaaagcc tttccgccgc aatacttccc tttttcagac
     1561 ggcatgtttc gtttacaatt cgggctgttt ccccctttgc gaaccgccat gcacatcctg
     1621 ttgaccgccc tgctcaaatg cctctccctg ctgccacttt cctgtctgca cacgctggga
     1681 aaccggctcg gacatctggc gttttacctt ttaaaggaag accgcgcgcg catcgtcgcc
     1741 aatatgcgtc aggcaggcat gaatcccgac cccaaaacag tcaaagccgt ttttgcggaa
     1801 acggcaaaag gcggtttgga acttgccccc gcgtttttca gaaaaccgga agacatagaa
     1861 acaatgttca aagcggtaca cggctgggaa catgtgcagc aggctttgga caaacacgaa
     1921 gggctgctat tcatcacgcc gcacatcggc agctacgatt tgggcggacg ctacatcagc
     1981 cagcagcttc cgttcccgct gaccgccatg tacaaaccgc cgaaaatcaa agcgatagac
     2041 aaaatcatgc aggcgggcag ggttcgcggc aaaggaaaaa ccgcgcctac cagcatacaa
     2101 ggggtcaaac aaatcatcaa agccctgcgt tcgggcgaag caaccatcgt cctgcccgac
     2161 cacgtcccct cccctcaaga aggcggggaa ggcgtatggg tggatttctt cggcaaacct
     2221 gcctatacca tgacgctggc ggcaaaattg gcacacgtca aaggcgtgaa aaccctgttt
     2281 ttctgctgcg aacgcctgcc tggcggacaa ggtttcgatt tgcacatccg ccccgtccaa
     2341 ggggaattga acggcgacaa agcccacgat gccgccgtgt tcaaccgcaa tgccgaatat
     2401 tggatacgcc gttttccgac gcagtatctg tttatgtaca accgctacaa aatgccgtaa
     2461 cgaaaataaa aatgccgtct gaacaatttc agacggcatt ttgtcatttg acgatttccg
     2521 acagcggcca gcgcgggcgt acattaaatg caccggcttc cctacccgtt ttcaaatgca
     2581 tcgcgcctgc aaaggcaatc atggcaccgt tgtccgtgca gtatgccgtc ggcgggaaaa
     2641 acacgctgac tttttcggac ggatgtttcg gcttgcctgt tggggtcggg atttgcaccg
     2701 tcatgctgcc gaaagtttca cggagtttgc ggtttgcacc gaccccgccg gcgaccacta
     2761 cggttctgaa ccctgtctgc aacagggctt ttttcacttt cgccgccaac acatcgacca
     2821 ccgcatcttg aaacgcacgg cagatgtcgt tgcgtgtctg ctcaggaatg tcatccgccc
     2881 cgttttccgc gcgcactttc tcaacggcgg tcaatacggc ggttttcaaa cctgaaaaac
     2941 tcatctgcaa atcgtcggaa tgaatcatcg ggcgcggaaa aacaaacgct tcgggcctgc
     3001 ccgattccgc aagttccgac agtttcgcac cgcccggata cggcaagccc agcagtttcg
     3061 ccgttttgtc gaatgcctcg cccgccgcat catcgacgct ctcgcccaaa agcgcgtagt
     3121 cgcctatgcc cctgaccgcc ataatctgcg tatgcccgcc cgaaaccagc agcgcgacaa
     3181 aaggaaagtc gggtttttcc tccgccaaca gcggcgacag cagatgtcct tccaaatgat
     3241 ggacgggaat aacaggcttg tccaacgcta aagccagcgc gttggcgtag ctcgaacccg
     3301 ccagcagcgc accgcccaaa ccgggcccct gcgtaaaggc aaccgcgtca atgtcgccat
     3361 acgatgcgcc cgcctgcgcc aaacagcctt cagtcaacgg aacaaggcgg cggatatggt
     3421 cgcggcttgc caattccggc acaaccccgc cgtattcggc gtgcattgcc atttgagtgt
     3481 gcaggcagtg cgcccgcaat ccacgttccg tatcgtaaag cgcaacgccg gtttcgtcgc
     3541 aagaagactc gattcctaat accaacatgg tctgatgccg ttaaaaactg aaaaacgtat
     3601 tttagcggat ttcggcacga ctgccgtatc ccaaaaacgg aacatgccgt ctgaagaccg
     3661 ttcagacggc atcgtcgcac cgtatcaaag cgttccgtaa gaatgcagcc cgctcaaaaa
     3721 catattcacg ccgataaagg caaatgcggt tacgaacaaa ccgataatcg cccaccacgc
     3781 cagcactttg ccgcgccaac cggcaaccag ccgcaagtgc agccaaacgg cgtaattgag
     3841 ccagacgatg aacgcccacg tctctttcgg atcccaactc caatagcgtc cccaagcatc
     3901 tgccgcccac agcgcaccca aaatggtggc aatggtaaag aacagaaagc cgacggcaat
     3961 cgccttatac atcacctcgt cgatcaatgc cgacggcggc agccacagtt ttccgccttt
     4021 tccttccgca cgcagggaaa ccagttcggc aataccgagc atcgcggaaa tgcaaaacgc
     4081 gccgtaaccg ataaagtttg ccggaacgtg gattttcatc caccaagatt gcaatgccgg
     4141 aatcagcggc tggatggtat gcgcctcgcg ggacacgctg taccacaaga caaatccaac
     4201 cacgaccgcc ataaagccga acacgaagcc gcccaatttc tgtatggcga acttaccttc
     4261 ataataaaga tacatcagcg cggtaatcac caaaaacagg atgaacactt catacaggtt
     4321 ggaaaccgga atatgccccg catcgggacg gagcagatag ctttcgtgcc aacgtaccag
     4381 cagaccggta aagcctgcta cggcagacac ccatgcaaac acggttccca tacccaacag
     4441 cgtgttggtc ggcacatttt ttacgcttgc caaaaccgcg cccgaaatat aggcgaacag
     4501 ggcgaagaag acaaaggcgc actgccacat aatcgccgac tggctgctga ggaaataccg
     4561 caacaggaaa atctctgccg atttgatgtt gccgccatac agaccgacgg cggcataggc
     4621 aagcaatacg cttaaaggaa caaaccagcg catcggtttg aaaaaccaac ccaaaaacac
     4681 ggcaataccg gcacttgccc acaacatgac cgtttcgtaa atgtccatat gcataccgga
     4741 acgggtctgt acgaaaaccg tagccgcaaa aaccagcacg gcaaataccc aatcccaaag
     4801 attcagattg ctgatcaaag acttctgaat cagcagctcg tgttccggaa gggttttata
     4861 gtgttcagtc atgattcaag tccttgccga gccgttgcag actctcgacg tgttttggaa
     4921 attccttctg caaatcccgt tcgctgcggg ccgaagacat ggcaaaacgg attttgccgt
     4981 ctgaaaacaa tacccacgcc cgtttttcgc gcacataaaa catcaatacc gtacccaata
     5041 ccaacagcac cgagccgaga tagaccaaaa gcgcacccgg ggaacgggtc atctgcaaac
     5101 ccgacgaacg cacctcggaa aacccatcaa gttgcagcag cataggcgcg ggatattcgg
     5161 tcaaacccgt gtacgcatcc atactgtgca gcaggaaacg gttccgcgct tcatcctgct
     5221 gccattcggg caagccgtac cggcgtatgg tttcatccaa agcagcgttc atcacgccgt
     5281 aaagcatttc gtagaaatag ccctgcatct tatcctgctg ctctttcggg atattggacg
     5341 taataaattc gtccaatccc aaatagcctt tttgtgcaaa gatgttcagc gtgttttccg
     5401 cagccagcat gaattgttcg cggatttcgg caggtgcgcc tttggttgcg tcggcaacca
     5461 gacgtttgcg cccttcccca tctttcaaaa actcacgcaa tgccataaag gtgtccgctt
     5521 tcaactgctt gtccaagggg atacgcagcc agcggtattg ctgctgcaag ccgctgcgcg
     5581 tgccggtaat ccaaaaataa tcctgttcct gcaaaaccgg cagcatatag tttttatatt
     5641 cgaccgcctg ccctgccgca tcacggatac ggtaaacaat ggaagggccg atattggtgt
     5701 attttttacc ttcctgagta acggcgcgga catcgttcag cgtggatttc aggctttttt
     5761 cccgttccgc gccctcgctc atgtcctcca cattcataga agtgaactga tcgaactcaa
     5821 gacgatattt gtgtttgcca atttccaacg gaaactggtg tatggatgtt gccttcaaca
     5881 cgacaggctc gcgcgaagca tcacccaaat tccacgcctt gaatgtcaaa tccgaaccgc
     5941 cgtcggcaaa actcgcctga taaatcgtga tgccgtgcaa ggtcaaagga tggttcacgc
     6001 ggatggtgcg ctcgagtttc tcaccggttg ccttatccgt tacttcaata tcactggcaa
     6061 aatcgcgcgg catacccgta ttgtaaaaat cgatatggaa ttttttcagt ttgacttcaa
     6121 aaggcaagtc ctgaaccaat atcccgttgt cggcattcag gaaaaccaca tccgcactct
     6181 gcccctcgga aatattgacg ttgcccctaa atgagagatt ggacgcactc aaaatacttt
     6241 cgggcttgaa atccttggca taaaccgcct gattgtccgg aacaatccga ccggtcagca
     6301 tacccagttt caacagcagg ttactgtcta tcaacccgcc caggcaaatg acaatcaaag
     6361 caacatgggc aaagatatag ccccatttgt tcatcgtgcc ttttttggcg gcaatcagaa
     6421 ccgacccgcc ctcacggctg acggtttttc cctgaaaacc ctgcacctcc agataacgtt
     6481 tggcaacctc gggcgcaatt tttacatcca acagcgaaga atggcgcatc gccgccagag
     6541 atttttcttt aaccttttcc cgaaaagact tcatttcgcg ccagaacggc ggcacattgc
     6601 gaatcaggca caaactggta gaaaccacca aaaacatcat gataacgaca aaccatgccg
     6661 aagcatagac gtcatacagt cccagaaaac caaaaatctg cgcccaaaaa ggtccgaatt
     6721 tgaccaaata atccgtctgc ggctggtttt gctgcaacac cgtaccgata accgatgcaa
     6781 tacccagcag actgagcaaa gcgactgcaa agcgcatgga gctgaaaaaa gcgaaccacg
     6841 gacgggaaag aagtggggga gatctacggg atttactcat tgtgtgttta ttccgccatc
     6901 aggaatatgg gaaagcagaa ttgggcaaac agaaaacaac gtcccgattc tactgtcttg
     6961 atgcttttta tttcaagaca atgaagacag cctgcatcga ttccaacggt tgcgattgaa
     7021 aaaacttatc gcagaattgc ctgaagccgt ctgaaaactt ttcagacggc ctctaaaaca
     7081 gactattgcg gaattaacgc aaaccttgga taaagttggc gaccgctttc aaatcttctt
     7141 cagacatacg gtttgcaata tcttccatga tggtattttt acgctgaccg gacttgtagg
     7201 cattcatctg ttcaacaata tatgcctgat gctgaccgcc caaacgcgga taagcctgaa
     7261 tttcgcttcc gcctcccggc atacccgcac cgctcggacc gtggcaggac atacacgccg
     7321 gcactttttt atcgctcaaa ccgccgcgat agattttcgc gcccaattcg ggattttcct
     7381 taggattggc ttcaccggat ttgggctgct gtttggcata gaatgcggat acgttcaaaa
     7441 tatcctgatc gctcaaattc attaccatcg gtttcatcac agctgccgaa ccgtgggtgc
     7501 gtttgccgtc tcggataccg atggtttgat gatagatgta agcagtatgc tgtgccgcca
     7561 aacgcggata catcgcaatg ccgctgttac cgtctgctgc atggcaagcc gcacaaaccg
     7621 ttgcggcaac ctgtttgcct ttttccacgt ctgctttggg agaggcggaa accgcaccgg
     7681 cagccaaaac aaaggccaat aaagtcaatc gtttcatgga gtgctcctga ttacagcatt
     7741 ggataacgca acaatgctct ttttatattc aaatacggga tttttgaccc gattaaaacc
     7801 gatgattctg taaacgtgtt attctatact aaatttacat taaattacca ctgtgtttca
     7861 cataaaacca accgcatatt tttgctgtcg gacaaacggc ggcggaaaac aaggatatgc
     7921 ccatgaacct ttttcaaaac gccaaattct tcacgacggt caaccacctt aaagacctgc
     7981 ccgacacccc tctcgaaatt gcctttgtcg gcaggagcaa tgccggaaaa tccagtgcca
     8041 tcaataccct gaccaaccat gtccgtcttg cctacgtttc aaaaacaccc ggacggacgc
     8101 agcatatcaa cttcttcgag ctgcagaacg gcaattttat ggtcgatttg cccggctacg
     8161 gttatgccca agtccccgaa gcagtacgcg cacattgggt caatctgctc ggcgactatc
     8221 tgcaacagcg caaacagctt atcgggctgg ttttgattat ggatgcccgc catcctttaa
     8281 aagaactcga catccgtatg ctggattttt tccacacgac cggcagaccg gttcacatcc
     8341 tgctgtcaaa agccgacaaa ttatccaaaa acgaacagat aaaaaccctg tcccaagtca
     8401 aaaaactgct caaaccttat tccgacaggc aaaacatcag cgtacagctg ttttccagcc
     8461 tgaaaaaaca aggtattgac gaggccaacc gaactgtcgg aagctggttt gacgcagcag
     8521 atgccgccgc ttcctctcca gaggaaaact gaccccaatt atacggaaac cgtatttccc
     8581 ccacttgacc gaccgcaaac atttaaaaaa ttgccactgc caaatctaaa atgccgtctg
     8641 aaagactttt cagacggcat tttgcggagt ctttaaaaca gagaatccaa ctgctgctgt
     8701 ttggaaccag tattactcgg aagcaccggc gtttcctgca tatcttggcg gacttcgtca
     8761 tccgccgcct gccgtccgcc ttctgccgcg cccccgtcat cttctttttc ccgtcgggaa
     8821 ggttgcggcg caataccgct gttgtccagc gtcaagcccg gatcggttac catacgttcc
     8881 ttcatatagt attcgccatt gctgctgacc acaccttcag gcattttcat ccccttgccc
     8941 tgctttcctt tcaacgcaaa acgcatatag tccacccaaa ccggcaccgc aatcgtaccg
     9001 ccgtagccga cacgccccat actcttaggt ttgtcgaagc cgatatatac ggcagtaacc
     9061 acatcagggt taaaaccgac aaaccacgca tccttattgt cattggtcgt acccgtttta
     9121 ccggcaatat ccgttcttcc caacgcagct gccccccttg ccgtaccaac acggaccaca
     9181 tcctgcataa tcttatacat aatataggca ttgcgcggat cgattgcctg aggcgcattt
     9241 tgcccggcca ccaaaggttg catttgggcg cgcaacctgc cgtctctgtc ataaatctta
     9301 tcgattacgt gcgaagaaac cctatatccg ccgttcgcaa atacgctata tgcctccgcc
     9361 actttcaacg gcgttgtctc gcccgtacct aaagccatag acaggcttgc cggcagctcg
     9421 gacgacctga agccgaaacg ccggatatac tgttgcgcgt aaccgacacc gatagacatc
     9481 aaaatacgga tggaaaccat attcttggaa gccgtcagag cctgtctcaa agtaatgtag
     9541 ccggaatatc tgccgtctga atttttaggt gtccaaaccg aaccgttcgg ccctttcccc
     9601 ggcagggaaa tcggcgcatc gttaaccact gtggacgcgg tcatcccctt agataatgcc
     9661 gccgaataga caaacggctt aaaggtcgaa cccggctgcc gcattgcctg aacggcacga
     9721 ttgaatgttt tgctgtgaaa atcataaccg ccgaccagcg cgcgcacagc tccggttttt
     9781 gcatccagcg aaaccaaagc cccctgcagc aacggctctt gaaccaccgc ccaacgcccg
     9841 ccgttgtttt tgacacggat gaccgcgccc ctgcggatac ggtcctcccc cattttttca
     9901 ttattgaccg cgcgggccgc aaaacccaag gcgcgcctgt caagcgtaac ccgcctgccg
     9961 ccgggcagct gtatgacgac atttttcttt ttagtcacat ccaacacaac ggcgggaacc
    10021 attttatcga cggtatagag tcccgacaga tactggctga cagtctcctc gacatcttca
    10081 ctcttactca aatcgatata gttttccgca ccgcggtagc tgctgccgcg atcgaaattc
    10141 cgtagagcct tgcgcaatgc ctcggttgcc accttctgat gatcggcgcg gaccgtggta
    10201 taaaccttaa aaccctgcgt ataggcatct tcaccgtatt tctcatacag ttcctgacgc
    10261 accatttccg ccacatataa agcactctga tcgattttcc gaacaaaccg ctcgtaatgc
    10321 agttcctcat tcaacgcctg atcgcgctgt tgcacggtaa tcatcttctc ctcgagcata
    10381 ttgttcaaaa tatacttctg gcgcaacttg gcacgttctg gattaacaat cggattatag
    10441 gcagacggag ccttgggcag tcccgcaagc atggcggctt ccgccaaagt caaatctcgg
    10501 acattcttat tgaaatagat ttgcgcggca gatgcaaaac cataggcgcg ctgaccgagg
    10561 taaatctgat tgaaatacaa ctcgaggatt ttgtctttgc ttaaagactg ctcgatttta
    10621 taggcaagca acacctcatt gaatttgcgt gtgaacgttt tttcactgct caaataaaaa
    10681 tttttcgcca cctgctgcgt aatcgtactc gcacccgact gcacgctgcc ggacacgaca
    10741 ttgccgacgg cagcgcgggc aacaccccaa acatccaccc cccaatgccg gtaaaagcgt
    10801 ttgtcctcgg cggcgataac cgcattccgc aacacctctg ggaaatcgcc gatttttgta
    10861 aattcgcgcc gctgctcccc atacataccg atgacttccc catccgccga ataaatagtc
    10921 aacggcattt taggctggta atgctgcaaa gaatccaaag acggcagttt cggatacgtt
    10981 accaaaatag caatggcaac caaacccact ccaaatacac aaaacccaaa aaccaaacca
    11041 aaacaagtcg ttaaaatctt tttaatcata gctgaataat aatttaccat tattggtatt
    11101 aaataaagta aaatagcaac cgatttctac aaagcacggt ttcaatgtgc aaagaacaag
    11161 gaatccatta cggataccga aacggttact cactgtacaa ataaagcagg aactttcatc
    11221 atgcgcttgt ttaaaagctt gaaaaaccct aaaaaaacag atgccaagct ccctaaaaaa
    11281 tcttcgggac tcaataaccg cgcggcaatc ggcatcgata tcgaccagca ttccatcaaa
    11341 atggtccaat tgtcaggacg tagtttaaac caaattcaat tggaaaaata cgtcattgcc
    11401 aaattaccaa agaatatcat tcaaggcaat aaagtccaaa attacgatca acttgttaca
    11461 tatttgcaac aagcctatgc caaactgggt acttcgtgca aaaacatcat cgcgtccgtc
    11521 ccgcaaaatc tggcaaccat cgaacaattg acctacacag acaaagatgc agaattagac
    11581 ctgcaggggt tcgtggagtc ctccatctcc gaagtcagct cgatatcgct cgaagaagcc
    11641 aattacgact atcaggtctt gtcccaatcg gccgccggcg aagctgtgtt ggccgtcgca
    11701 tcgagaaagg atgaaatcga acccctgatt gacgcattca acgccgccgg tatgaaatta
    11761 tccgcgcttg atgtggacat tttcggacaa tacaacgcct acgcgctatg gataaaccat
    11821 ttcgcccccg agcttgcagc cgaaaaagtc gccattttcg gcgtatatgc cgcacagacc
    11881 tacgccttgg tcatccaaga cggaaaaatc ctatacaaac aggaaacctc cgtcagcgaa
    11941 gaacagctca accaactcat ccagcacacc tatcaggtaa cagaagaaaa agcggaagaa
    12001 atcatcaact ccccgcaaaa accttccgat taccaagaaa gcgtggcaaa ctatttcaac
    12061 cagcagatta cccaagaaat acaaagggtc ttgcagtttt attacaccac gcagaccgca
    12121 gacgatatga ccgacatcaa gcatatcctg ctgaccgggg aagcggcgcg ccaggaaggc
    12181 atcgcccaaa ccgtcgcctc acaaaccaat gcagatgtac aatgcgtcca tcccgcgcgt
    12241 tattttgcgg acaacctcaa aacagacaaa caacaattcg aacttgatgc gccgacactg
    12301 accagggcgt tcggtttggc ggtacgggga ttataattat gaacaattta atcaaaatca
    12361 acctcctccc ctacagggaa gagatgaaca agcgcaaaca gcagcagttt aaaacgctga
    12421 tgtacggtgc cgtgctgacg ggcgttgccg ccgttgcggc aacctacctg tttatcgaca
    12481 atatgatcaa taaccagtcg gaaagaaaca cgctgctgga aacctccatc gcacacttgg
    12541 ataccgagct gtcggaaata caaaagctca aacaggaaaa agatgccttc ctgattaaga
    12601 aaaacaaaat cgaggagctc cagctcaaac gcctccaagc cgcaaaaatc ctcgacagcc
    12661 tgaatgaggc cgtccccgga agcacctacc tgacctcgct ggatgccgtt accgccgact
    12721 cttatcggct cagcggcagg acatccagcg acaaccgcgt tgccgccatg atgagggcga
    12781 tgcccaatac cggcatattc aagcaacccg aattgttaag catcaagaaa aacaattcgc
    12841 atcaagaatt tacccttcag gcaacattac aacccatcgt aaaggcggcc gaatccaaag
    12901 agaatccggc ttcgggaaac gcacaggagg caaactgaat ggcttctaaa tcatctaaaa
    12961 ccaacttgga tctcaacaac cttcacctgc tcaaccttcc tgccaggctt tttatcgccc
    13021 tgctggccgt tgccgccgtg ctggggctcg gttatgccgg attgttcaaa agccagatgg
    13081 aatcccttga ggaatacgaa gcaaaagaaa ccgaactgaa aaacacctac aaacagaaaa
    13141 gtatcgacgc ggccagcctg aacaacctga gggacgaact tgcctcaatc cgctctgcct
    13201 tcgatatcat gttgaaacag ctgccgacag atgcagaaat tcccaatctg gttcaagagc
    13261 ttcatcaggc aggttcgagc aacggtctgc gcttggacag cgttatgccc caacctcccg
    13321 tagatgacgg ccccatcaaa agattaccct attccatttc cattaccgga aattacgaac
    13381 agatcagcca atttacccgc gatgtcggca gcctctcccg aatcattacc cttgagtcgc
    13441 tgaaaatcgc ccaatctccg gaaaacggcg gcaatcctga cggcaagagc agcatcctga
    13501 acctcagcgc cattgccacc acctaccaag caaaatccgt agaagagctt gccgcagaag
    13561 cggcacaaaa tgccgagcaa aaataactta cgttagggaa accatgaaac actatgcctt
    13621 actcatcagc tttctggctc tctccgcgtg ttcccaaggt tctgaggacc taaacgaatg
    13681 gatggcacaa acgcgacgcg aagccaaagc agaaatcata cctttccaag cacctaccct
    13741 gccggttgcg ccggtataca gcccgccgca gcttacaggg ccgaacgcat tcgacttccg
    13801 ccgcatggaa accgacaaaa aaggggaaaa tgcccccgac accaagcgta ttaaagaaac
    13861 gctggaaaaa ttcagtttgg aaaatatgcg ttatgtcggc attttgaagt ccggacagaa
    13921 agtctccggc ttcatcgagg ctgaaggtta tgtctacact gtcggtgtcg gcaactattt
    13981 gggacaaaac tacggtagaa tcgaaagcat taccgacgac agcatcgtcc tgaacgagct
    14041 aatagaagac agcacgggca actgggtttc ccgtaaagca gaactgctgt tgaattcttc
    14101 cgacaaaaac accgaacaag cggcagcacc tgccgcagaa caaaattaag aagaggatta
    14161 ctccattact gacaaaaatc atttccggtc tctttgtcgc aaccgccgcc tttcagacag
    14221 catcggcagg aaacattaca gacatcaaag tttcctccct gcccaacaaa cagaaaatcg
    14281 tcaaagtcag ctttgacaaa gagattgtca acccgaccgg cttcgtaacc tcctcaccgg
    14341 cccgcatcgc cttggacttt gaacaaaccg gcatttccat ggatcaacag gtactcgaat
    14401 atgccgatcc tctgttgagc aaaatcagtg ccgcacaaaa cagcagccgt gcgcgtctgg
    14461 ttctgaatct gaacaaaccg ggccaataca ataccgaagt acgcgggaac aaagtttgga
    14521 tattcattaa cgaatcggac gataccgtgt ccgcccccgc acgccccgcc gtaaaagccg
    14581 cgcctgccgc accggcaaaa caacaggctg ccgcaccgtc taccaagtcc gcagtatccg
    14641 tatccgaacc ctttaccccg gcaaaacaac aggctgccgc accgtttacc gagtccgtag
    14701 tatccgtatc cgcaccgttt agcccggcaa aacaacaggc ggcggcatca gcaaaacaac
    14761 aggcggcagc accagcaaaa caacaggcgg cagcaccagc aaaacaaacc aatatcgatt
    14821 tccgcaaaga cggcaaaaat gccggcatta tcgaattggc tgcattgggc tttgccgggc
    14881 agcccgacat cagccaacag cacgaccaca tcatcgttac gctgaaaaac cataccctgc
    14941 cgaccacgct ccaacgcagt ttggatgtgg cagactttaa aacaccggtt caaaaggtta
    15001 cgctgaaacg cctcaataac gacacccagc tgattatcac aacagccggc aactgggaac
    15061 tcgtcaacaa atccgccgcg cccggatact ttaccttcca agtcctgccg aaaaaacaaa
    15121 acctcgagtc aggcggcgtg aacaatgcgc ccaaaacctt cacaggccgg aaaatctccc
    15181 ttgacttcca agatgtcgaa atccgcacca tcctgcagat tttggcaaaa gaatccggga
    15241 tgaacattgt tgccagcgac tccgtcaacg gcaaaatgac cctctccctc aaagacgtac
    15301 cttgggatca ggctttggat ttggttatgc aggcacgcaa cctcgatatg cgccaacaag
    15361 ggaacatcgt caacatcgcg ccccgcgacg agctgcttgc caaagacaaa gccttcttac
    15421 aggcggaaaa agacattgcc gatctgggcg cgctgtattc acaaaacttc caattgaaat
    15481 acaaaaatgt ggaagaattc cgcagcatcc tgcgtttgga caatgccgac acaaccggaa
    15541 accgcaatac gcttgtcagc ggcaggggca gcgtgctgat cgatcccgcc accaataccc
    15601 tgattgttac cgatacccgc agcgtcatcg aaaaattccg caaactgatt gacgaattgg
    15661 acgtacccgc gcaacaagtg atgattgagg cgcgtatcgt cgaagcggca gacggcttct
    15721 cgcgcgattt gggcgttaaa ttcggcgcga caggcaagaa aaagctgaaa aatgatacaa
    15781 gcgcattcgg ctggggggta aactccggct tcggcggcga cgataaatgg ggggccgaaa
    15841 ccaaaatcaa cctgccgatt accgctgccg caaacagcat ttcgctggtg cgcgcgattt
    15901 cctccggtgc cttgaatttg gaattgtccg catccgaatc gctttcaaaa accaaaacgc
    15961 ttgccaatcc gcgcgtgctg acccaaaacc gcaaagaggc caaaatcgaa tctggttacg
    16021 aaattccttt caccgtaacc tcaatcgcga acggcggcag cagcacgaac acggaactca
    16081 aaaaagccgt cttggggctg accgttacgc cgaacatcac gcccgacggc caaatcatta
    16141 tgaccgtcaa aatcaccaag gactcgcctg cgcaatgtgc ctccggtaat cagacgattc
    16201 tgtgtatttc gaccaaaaac ctgaatacgc aggctatggt tgaaaacggc ggcacattga
    16261 ttgtcggcgg tatttatgaa gaagacaacg gtaatacgct gaccaaagtc cccctgttgg
    16321 gcgacatccc cgttatcggc aacctcttta aaacacgcgg gaaaaaaacc gaccgccgcg
    16381 aactgctgat tttcattacc ccgaggatta tgggtacggc cggcaacagc ctgcgctatt
    16441 gatgcgtcaa aataagggca tatgttttac ggcatatgcc ctttctttat gctttttgcc
    16501 gcgaccgaaa tgccgtcatt cccgcgcagg cgggaatcca acttgtccgg ttccggttgt
    16561 ttttcgtctc gtaacttttg agccgtcatt cccgcgaaag cggaaatcca gtccgttcgg
    16621 tttcggtcgt ttccgataaa ttcctgctgc ttttcatttc tagattccca ctttcgtggg
    16681 aatgacggtg gagaggtttt tgttgtttcg gagaagtttc tgcaacccta gaatctcgtt
    16741 atttccacaa aaaacagaaa cccgaaacag caacttaaaa cctcgtcatt cccgcaaaag
    16801 cgggaatccg gtccgttcag tttcggtcgt ttccgataaa ttcctgctgc ttttcatttc
    16861 tagattccca ctttcgtggg aatgacggcg gagaggtttt tgttgtttcg gagaagtttc
    16921 tgcaacccta gaatctcgtt atttccacaa aaaacagcaa cccaaaacgg caacttaaaa
    16981 ccccgtcatt cccgcgcagg cgggaatcta ggtctgtcgg ttcaggaact tatcggataa
    17041 aacggtttct ccaaccctgc gttctagatt cccactttcg tgggaatgac gggatatggg
    17101 tttccgtgcg gacgtgttcg gatttccgcc tgcgcgggaa tgacggcgac agatgccc
//
LOCUS       DAWKOG010000026        17058 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.026, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000026 DAWKOG010000000
VERSION     DAWKOG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17058
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.026"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(16..771)
                     /locus_tag="WJK66_000668"
     CDS             complement(16..771)
                     /locus_tag="WJK66_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEZ7578416.1"
                     /translation="MKFTRLLFLCAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASIGSS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCASR"
     gene            complement(951..2111)
                     /gene="nirK"
                     /locus_tag="WJK66_000669"
     CDS             complement(951..2111)
                     /gene="nirK"
                     /locus_tag="WJK66_000669"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase, copper-containing"
                     /protein_id="HEZ7578417.1"
                     /translation="MKRQALAAIIASMFALAACGGEPAAQTPAASAEAASSAAQTAAE
                     TPAGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMKMDDGVEYRYWTFDGD
                     VPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTSTFSFK
                     ALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKGKKGA
                     QGLQPFDMDKAIAEQPEYVVFNGHVGAIAGDNALKAKAGETVRMYVGNGGPNLVSSFH
                     VIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFRAFNKG
                     ALGQLKVEGAENPEIMTQKLSDTAYAGNGAAPAASAPAASAPAASAPAKSDY"
     gene            2481..4736
                     /locus_tag="WJK66_000670"
     CDS             2481..4736
                     /locus_tag="WJK66_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HEZ7578418.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQSTGGMEVGSILGHGAYQAPDWTADWLHRELSAWLDLTAQQ
                     TYGKKFDEVSPEEQAVLKTRLADEYRNQSRIKEDGSVVISDTRAKAIESILPYYHGVY
                     GDDPALQTTREHFAMKNNTLPSQEAREKLFDFFFWTSWSASTNRPGEVFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNAQFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGVIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAADLVFIGGAICVAI
                     QATKIVFGRDK"
     gene            complement(5000..5704)
                     /locus_tag="WJK66_000671"
     CDS             complement(5000..5704)
                     /locus_tag="WJK66_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7578419.1"
                     /translation="MDFLERLKSLWPDNAKPADFYNKIDMSASGFSRVWKDGAIPTAD
                     YLIKIQEVTGCDLNWLLTGQGSPYMNKGQAVEVRTHTDGTATDTLGNPINLDEFVFIP
                     RYDVYAAAGHGYPAEDDKPLFCMAFRRYWIENYVTRQLDKLSVIAVKGDSMEGVLNHG
                     DNILVNHAETTPRDGLYVIRIDNHLFVKQIQKLPGKLLVKSANPVYEPFEIDLTDDNQ
                     NVAIIGRVEWYGRTVN"
     gene            5921..6184
                     /locus_tag="WJK66_000672"
     CDS             5921..6184
                     /locus_tag="WJK66_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ7578420.1"
                     /translation="MQKNATPKNWHRADIVAALKKKGWSLRALSIEAGLSPNTLRSAL
                     AAPYLKGERIIAAAIGVEPEEIWPERYADRNLKPVFPKRVVNG"
     gene            6262..8307
                     /locus_tag="WJK66_000673"
     CDS             6262..8307
                     /locus_tag="WJK66_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ7578421.1"
                     /translation="MLISAVELAGLQLPKLPNSRQGIEYHAKKNNWPFEEVVGQARGG
                     KLKKYLVSALPSEIQAAIQEKQAATLLAKVPMLPAEVKKPLRQNKKMRQLGLIPCEEG
                     LARLDDRQTETAHARCAIVAYVLPLHELAGMPIKKAVAFVAAEAAAGRLPEDVAKLIP
                     LANARNNGERSLSEPTLYRWVRAYRAASDSMSRLLALAPVKTREKTPLLAIDWLPYFL
                     MFYQRPNKPTMMAAAKKLAQWYLEQGKIEQMPSYDQIQTVMKRLPEHMKERGRRTGSA
                     YKALLPYIDRDWTALKPNDVWVGDGHSFKAKIRHPMGYLFTPEVTMIVDGCSGAVVGW
                     SVALSETAVAVADALRHGMTHFPPPLGYYSDNGSGETGDMLDKETTGILPRIGIEHFT
                     GIPGNPQGRGKIERLWQTITIPLAKQYATYQGKDADAETLRKVSNALASAQKVQKKGK
                     ELSKAQEAALSAAPTWAQFIADLEEAVRRYNFEHEHRSLPKNPEAGRHFTPMKYYEYR
                     METDGMRVKTDVLSPLELDFMYRPEEERIPDRGAVSLHNNTYFHQELLDYSGQKVRVA
                     YDIHDADHVIVKDMQGKVICKAVFNGNKRAAFAETRMEQLAERRRKGQAKRLQDKMDL
                     IEAQRQSAAPIIEQQPDFGEFLKLETESGGLVEVENRPSESGRGKKKLRDFLWEDAG"
     gene            8349..9263
                     /locus_tag="WJK66_000674"
     CDS             8349..9263
                     /locus_tag="WJK66_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ7578422.1"
                     /translation="MIEKLRQFLETSGMSQNKAADRMGVSRSALSGYLNGKYDGDIAG
                     MDKKAALFLEQEGDRAELKKLDIPYVETGTAKKMKGWLGLAAWLGQLGIVYGGAGLGK
                     TTVLKQYAATNPLALLIEPDTGYTAKVLLQEICHALDLSERGNIHELTERIINCLKRD
                     KKSRSPRDAHRILLIDEAEQLPTRALESLRRIHDKSGVAVALVGMPKLLLNLKGPNSE
                     FKQLFSRVSVKMELGEMLPETDLKQIAAAVLKTNDEAVLQKVVKTAKGNARKLSKLLL
                     IVDYLLQVNPDVDLDDDVIEHAETYLIH"
     gene            9339..9581
                     /locus_tag="WJK66_000675"
     CDS             9339..9581
                     /locus_tag="WJK66_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578423.1"
                     /translation="MENLKMRHLLAIPAIVIAVTACANRCTEPVPAWSLYQNETSQQR
                     LMREKQNAADFAVLQAEKAYERMSDKERMKGVVYEN"
     gene            9571..9870
                     /locus_tag="WJK66_000676"
     CDS             9571..9870
                     /locus_tag="WJK66_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578424.1"
                     /translation="MKTETKICKKCGEERPLFRFKKDDAYAGGYRPVCYECLNARAKE
                     LKAVRDSMAERKIISKGISINGGKTHMEFPEEPEFMKNKYWTVTKTDYRRPLKGL"
     gene            9873..10025
                     /locus_tag="WJK66_000677"
     CDS             9873..10025
                     /locus_tag="WJK66_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578425.1"
                     /translation="MSKRLARRKMPKHELKRVKAFALKQAVEKIRARWGEKAIRLAST
                     DNKQTD"
     gene            10041..10205
                     /locus_tag="WJK66_000678"
     CDS             10041..10205
                     /locus_tag="WJK66_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578426.1"
                     /translation="MMNEMNAVAALLVIAILLAAVMTVLVEILVEQRVKERLGKLLDK
                     ACRPDDGWQE"
     gene            10186..10401
                     /locus_tag="WJK66_000679"
     CDS             10186..10401
                     /locus_tag="WJK66_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578427.1"
                     /translation="MTGGRNDKEGNVMNMVLTIYFWLVGLVCFARSAKETYYAESLYG
                     LLTGLLGVMCSAFVVSVSVARVMGWLK"
     gene            10398..10712
                     /locus_tag="WJK66_000680"
     CDS             10398..10712
                     /locus_tag="WJK66_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578428.1"
                     /translation="MNQLKMTIAKPETEDFEDAWAFIRMLNLVTYDLNPLKTDTDGEY
                     EYLADEDKSDVLDAVVEKFNECSLEWMLSALQALMSPEMGIINQDSDTLELHPKLKGG
                     TE"
     gene            10831..11208
                     /locus_tag="WJK66_000681"
     CDS             10831..11208
                     /locus_tag="WJK66_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578429.1"
                     /translation="MRYLTLFRPAEKDLSFNRVSKLVGEIAPMIRDGKIVRNRKTYPA
                     PREAWIWAATRCLEARDAGKLTPPLTSHGYLLENITFWSPEKTAGTAVLPSPQPSHTR
                     EVASTKLRSGLGGLMEWSNGGKQ"
     gene            11192..11647
                     /locus_tag="WJK66_000682"
     CDS             11192..11647
                     /locus_tag="WJK66_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578430.1"
                     /translation="MEENNSWLKKAIAQGFMMLAALNLKGRPASADLTAVAELWLVIL
                     SGRSWQPEHDGIRIQAAFRAIAASSSEWPNPADLIKHLPPGEVRMVPRLEKKHCPTEY
                     GKEQAAELKKIVGRLKNAPCMNRDWIHGQRHRSVDECKRIYAERQKGNK"
     gene            11619..11813
                     /locus_tag="WJK66_000683"
     CDS             11619..11813
                     /locus_tag="WJK66_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578431.1"
                     /translation="MPKGRREINSQMSTFFDNQESDLINEFLTEYWEEFEKFSANNGF
                     DDDLLKQISMELEGHRNHYE"
     gene            11806..12417
                     /locus_tag="WJK66_000684"
     CDS             11806..12417
                     /locus_tag="WJK66_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3164 family protein"
                     /protein_id="HEZ7578432.1"
                     /translation="MSNLDMKQYKQDAKGNLVPLANIKEIDLLRDELVQEIAAKARAV
                     QDNLMAFKREAMDDIAAFVQLSADRYDVSVGGKKGNISLHSFDGAYRVNLAIQDTLVF
                     DEGLIAAKALIDECINEWTEGSRTELKTLINAAFQVDKEGNISTARVLGLRRLQITDE
                     KWQRAMDALSDSLQVHISKPFVRVYQRGEDGEYQLMNLDVAKV"
     gene            12423..13073
                     /locus_tag="WJK66_000685"
     CDS             12423..13073
                     /locus_tag="WJK66_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578433.1"
                     /translation="MNANIMIYLIEVNDGPVAKALRSFQQDKAAMRQAWLDWAREHLP
                     SDALMREWSDGQVAGFAFPSGIPDGWKKPNKNGICWPRQNNPILKTMPLNKRFKRPEE
                     YLEEVGITAPTMIFEKNSDGETWASWGIGNFFNPVQFVWAGLEEDAPKGVVTPDYAYE
                     LREGAKRIRNGCTMQPPEDFDWQNLLPGCRVIPRYEWDYLVGKWQETRNDAEEQEA"
     gene            13074..13274
                     /locus_tag="WJK66_000686"
     CDS             13074..13274
                     /locus_tag="WJK66_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578434.1"
                     /translation="MAKIIIEIEDLPEGTDINFKGDLPAADAKDKTGAQQTAVLISKM
                     IQAAQMMASPSVNIRPSETANP"
     gene            13290..13598
                     /locus_tag="WJK66_000687"
     CDS             13290..13598
                     /locus_tag="WJK66_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEZ7578435.1"
                     /translation="MPHLNLNRSQKVNKSELIQAIADEAELSKRAAAEFVNAFVSVVT
                     QELKDGNDVTLVGFGTFHAAQSAERQGRNPKTGEPLTIAARKTPKFRSGKALKDAVNV
                     "
     gene            13666..14100
                     /locus_tag="WJK66_000688"
     CDS             13666..14100
                     /locus_tag="WJK66_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1018 domain-containing protein"
                     /protein_id="HEZ7578436.1"
                     /translation="MMNTNEQYKRKGLIAKIKIAQSQLGMEDDVYRAMLARVCGKTSC
                     TQMNLAELQAVAAEMKRMGFKQTAPKGKGIRPHLTQDRAALLNKLEALLTVGDKSWQY
                     ADGMAKRMFGKDLVRFLTPEQLYKLVQALQIHINKAKKAAEQ"
     gene            14097..14543
                     /locus_tag="WJK66_000689"
     CDS             14097..14543
                     /locus_tag="WJK66_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578437.1"
                     /translation="MIRVDEEDFEAVRHLLPESVLALITIIGLTETVELVKNLGGTTY
                     PLRQGRTKGSESRLAYLEEIVGGAAMEKMVEALAPCDLFIPKCEQALLELRDRHIRRR
                     FDAQTGKGVPAYEAVNDLALAHALTDRHIWRILKKPDNECEQGGLF"
     gene            14591..15232
                     /locus_tag="WJK66_000690"
     CDS             14591..15232
                     /locus_tag="WJK66_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578438.1"
                     /translation="MKTFYVLMLAAMLAACGGQENKPAAGESQTDNRAQQYQNQQKQI
                     AARLADEEYFIGENNLEQIRLHANLKERAQKLLSLLAQADKESRVWVLPGDAAKIKEF
                     NAAFAAVAKSAEESFGGPFLADKAGLYQCTAAANAAYDYFFARQRKDALTENYRQQYT
                     DNIAACQGQIRTPPAAQATVYARKGIRLPLNNCLPVLAGDEEFDTYTCPMEVK"
     gene            15364..15909
                     /locus_tag="WJK66_000691"
     CDS             15364..15909
                     /locus_tag="WJK66_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ7578439.1"
                     /translation="MSKIIVLTAGHSNTDPGAVNGSDREADLAQDMRNIVAAILRDDY
                     GLTVKTDGTGKGNMPLREAVKLIRGSDVAIEFHTNAAVSKAATGIEALSTVKNKRWCQ
                     VLSKAVAKKTGWKLRGEDGFKPDNAGQHSRLAYAQAGGIVFEPFFISNDTDLALFKTT
                     KWGICRAIADAIAMELGAARV"
     gene            15906..16112
                     /locus_tag="WJK66_000692"
     CDS             15906..16112
                     /locus_tag="WJK66_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578440.1"
                     /translation="MNIIGKLKEAASYFLTKLIGENPSNEQVNRALIQMPNVRPIHTY
                     PRPNLRNSGVAAAKRAARKRKNRR"
     gene            16115..16279
                     /locus_tag="WJK66_000693"
     CDS             16115..16279
                     /locus_tag="WJK66_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578441.1"
                     /translation="MGQVAFYEKMIGLWSAKSREASEQADLAAFEFAEGELANYQEML
                     KRHLQTKSVE"
     gene            16282..16518
                     /locus_tag="WJK66_000694"
     CDS             16282..16518
                     /locus_tag="WJK66_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578442.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTVKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSLVSVKRQEVENESRETAGE"
     gene            16490..16909
                     /locus_tag="WJK66_000695"
     CDS             16490..16909
                     /locus_tag="WJK66_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578443.1"
                     /translation="MNLVKLLANNWQPIAIIALVGTLLAVSHHQGYKSAFAKQQAVID
                     KMEKDKAQALLLSAQNYARELEQARAEAKKYEVKAHAVGMALAKKQAEVSRLKTENKK
                     EIENVLTQDRKNAGGGCIDGFGHHGLQLYKRALGYGN"
BASE COUNT         4409 a         3789 c         4905 g         3955 t
ORIGIN      
        1 ctataattga aggggttatc ggcttgcgca acggaagccc aagttgtgca agacatattt
       61 ggattgcagg ctggtgcgga tgccgtagcg gaggaaggcg gcatagttgg acgagtcgct
      121 cgacccaata gacgcgccgc tgcaaaacat ttgcgcattg gcatttccgg aggaaagcag
      181 gctgctgttg aaatcttccg tccattccca aataagtccg tgcatatcat aaacgcccca
      241 gtagttggga cggcccttgc cgacgtcatg caagcctttt cgtccgccgt cggcatacca
      301 atcgagaata gtgcggttgt agccgggttc gtttgagccg tttttctgcg tggcggaagc
      361 aagtccggca aattcccatt cgtcaatggt cggcaggcgt ttgccttgtg cggcgcaata
      421 ggcgttggcg gcaaaccagg aaacattggt taccggttgt tttaattcgc ccgccttcgg
      481 cgcatagctg cggctgccgt ttttcatcca atgcttcagg taagcgggtt ctgcctgttt
      541 ggaaccgatc ctgccttttt gccattgggg gtggctgttg acaaattcgg caaactcggc
      601 attggtaacg ggatatttat ccagtttgaa cggtttgact ttaatcaggc cggtatcttt
      661 tttcagataa agcgggcggt agctgccgcc ttcgatttga accatttcgg cagctgccgc
      721 ttgagtgccg gcgagtgccg cacagagaaa gagtaaccgg gtaaacttca tatcatgcct
      781 cctgacaggc agttaaaatt gatttttcag acggcatttt aaacaaaaag ccgcgaatgc
      841 cgtctgaaga atcaatcttc cgaaaggaaa gattggttgt taaaaaacca ctgccgtgcg
      901 taattaagta cggcggcagt ggttcgtttg cctaaaaatg ggtgttcaat ttagtaatcg
      961 ctttttgcag gagcagaggc tgcaggagca gaagctgcgg gagcggaagc agcaggagct
     1021 gcaccgttac cggcgtaagc ggtatcactc aatttttgag tcatgatttc agggttttct
     1081 gcaccctcta ctttcaattg acccagtgcg cctttgttga atgcgcggaa gatagagtgg
     1141 tcaaccaaag tgtagctgcc cgggatgtcg actttgaatt cgacgatggc agagccgccg
     1201 gcaggaacga tggtgctttg tacgttttcg ttaatcagtt tgccgccttc aacataaact
     1261 ttgtcgaaga tttcaccgat tacgtggaag gaagacacca agttaggacc accgttacca
     1321 acgtacatac gtacagtttc gcctgctttg gctttcaggg cgttgtcgcc ggcgatagca
     1381 cctacgtgac cgttaaatac aacgtattca ggttgttcgg cgatggcttt gtccatatcg
     1441 aacggttgca ggccttgcgc gccttttttg cctttggtgt agaagtcgcc ttggacgatg
     1501 tagaactctt tatccacttt cggcagacct tctttaggct cgaccaaaat cagaccgtac
     1561 ataccgttgg cgatgtgcat accgaccggc gcgacggcgc agtggtagat gtacagaccc
     1621 ggttgcaggg ctttgaagct gaatgtggaa gtacggcccg gagcggtaaa ggttgcggcc
     1681 gcgccgccac cttggccggt agctgcatgg aagtcaacgt tatggggaac ggtagaagaa
     1741 ggattgttag agaattctac ttctacggta tcaccttcgc gtacgcgaat catacggccc
     1801 ggaacgtcgc cgtcaaatgt ccagtagcgg tattccaccc cgtcgtccat tttcatggtt
     1861 ttttcgacgg tttccatttt tacgcgtact ttggcaggat agtcgcggtc gattgcggga
     1921 ggtacttcag gagcgtgggt ggtaactgca tcgatgacgg gcagttcgcc tgcaggcgtt
     1981 tcggcggcgg tttgtgcggc ggagcttgcg gcttcggcgg aagcggcagg ggtttgcgcg
     2041 gcaggttcgc caccgcaggc ggctaaagca aacatagaag cgataattgc ggctaaggct
     2101 tggcgtttca taatgttttc cttttgtaag aaaagtaggg agattggtta agaactggtg
     2161 tgattatgcg tttgccccat caccgacatt ttgatttaag tcaaataatt cataaaatat
     2221 gaacttaaaa tgatacttct aatactttat agctactaaa aattacaata aataggctga
     2281 tttgcttaag ggcttcggca aatcaggcaa aggcagggta gttgccgact aaagaataat
     2341 gatagttatt atcatatatg tatttgtttt atatgtgagt ttctttgaac aaatgtttat
     2401 ttgcgcggta aaccgtgcta caatctttaa cattcatatt ttgtgaattt taatccacta
     2461 tattaaaagg agctctcaaa atgggacagt acaagaagct gtggtacttg ctgtttgccg
     2521 ttctggcggt atgctttacc attcttggct atatgggcag cgaggtttat aagaaagccc
     2581 cgccttaccc cgaacaggtc gtttccgcat caggcaaagt gctgatgacg aaagacgata
     2641 ttttggcagg tcagtctgcg tggcagagta ccggcggtat ggaagtcggt tcgattctgg
     2701 gtcacggcgc atatcaggct ccggactgga cggcggactg gctgcatcgt gagttgtccg
     2761 cttggttgga tttgaccgcg caacagactt acggcaaaaa attcgatgaa gtttcccctg
     2821 aagaacaagc cgttctgaaa acccgactgg ctgacgaata ccgcaaccaa agccgtatta
     2881 aagaagacgg cagcgtcgtc atcagcgata cgcgcgcgaa agccatcgaa agcatcctgc
     2941 cttattacca cggtgtttac ggcgacgatc ccgcgctgca aaccacccgc gaacactttg
     3001 caatgaaaaa caacacattg ccaagtcagg aagcgcgtga aaaactgttc gacttcttct
     3061 tctggacttc ttggtctgct tcgaccaacc gtcccggcga ggtctttacc tacaccaaca
     3121 actggccgca cgagcctttg attaacaatg taccgactac tgaaaactac atgtggtcgt
     3181 ttaccagtgt tgtactgctc ttgatgggca tcggtttgtt gatgtggggt tattccttcc
     3241 tgaccaaaca cgaggaagtg gaagtgccgt ctgaagaccc gatttccaaa atccaactga
     3301 ccccttcgca aaaagcattg ggcaaatacg tcttcctgac ggttgccctg tttgtggtac
     3361 aagtattgct gggcggtctg actgcgcact acaccgttga aggtcagggt ttctacggca
     3421 ttgacgaggc gttgggcttt gaaatgtccg actggttccc ctacgccctg acccgtactt
     3481 ggcacatcca atccgccatc ttctggattg caaccggctt tttgacggcg ggtttgttcc
     3541 tcgcgccgat tgtcaacggc ggcaaagatc ccaagttcca acgtgcaggc gtaaacttcc
     3601 tctatatcgc cctcttcatc gtggtcggcg gttcttacgc gggcaacttc ttcgcgttga
     3661 cgcacatcct tccgccggaa tttaacttct ggttcggaca ccaaggttac gaatacctcg
     3721 atttgggacg tttctggcaa ctcctgctga tggtcggcct gctgttgtgg ctgttcctga
     3781 tgttgcgctg cacggtttcc gccttcaaag aaaaaggcgt ggacaaaaac ctgctggcaa
     3841 tctttgtcgc ttctatggtc ggtgtgggcg tattctacgc accaggcctg ttctatggcg
     3901 aaaaatcccc gattgccgta atggaatact ggcgttggtg ggtggttcac ctgtgggtgg
     3961 aaggcttctt tgaagtattt gccactgccg cttttgcctt tgtcttctac aatatgggct
     4021 ttgtccgccg cagtaccgcc acggcctcta ctctggccgc tgccgccatc tttatgttgg
     4081 gcggcgttcc gggtacgctg caccacctgt acttctccgg ctctacctcc gcctctatgg
     4141 caatcggtgc ctgcttctcc gctttggaag tcgtgccgct ggtgttgctg ggccgtgaag
     4201 catacgagca ttggtcttac caacacctgt ccgactgggc gaaacgcctg cgttggccgc
     4261 tgatgtgctt cgtggcagtc gccttttgga atatgatcgg tgccggtgta ttcggtttcc
     4321 tgattaaccc gccgatttcc ctgttctata tccaaggttt gaatacttct gctgttcatg
     4381 cgcacgccgc cttgttcggt gtgtacggtt tcttggcatt gggcttcgta ttgctggttg
     4441 cccgttattt gaaaccgaac gcgcagtttg acgacaaact gatgacttgg ggcttctggc
     4501 tgctcaacgg cggcttggtc ggtatgattg ccatcagcct gttgccggtt ggcgtgattc
     4561 aggcatacgc ctccatcacg cacggtctgt ggtatgcccg cagcgaagag ttcctgcaaa
     4621 tggaaatcct cgataccttg cgttgggtac gtacggcagc tgacttggtc tttatcggcg
     4681 gtgcaatctg cgttgccatc caagctacta aaatcgtatt cggccgcgac aaataagttt
     4741 tgcggtatga aatgagcaac ttatggggta ggtattattt tgacaccttt agaaagaaag
     4801 caaaagaagg cgaaaatttt aaaggaaaca aaaatttgaa cggaaattta ggaaaaagcg
     4861 gctttgacac ctttaggtgc aaagtaggta gaaagtgata taactttgat accttttttg
     4921 cacggttttg atgaggtttt gggttgattt tggttttatt tgaattgaac ggcgtttgga
     4981 atatccaaac gccgtttttt tagttgacgg tacggccata ccattctacg cgtccgatga
     5041 tggctacatt ttggttgtcg tcggttaaat ctatttcgaa aggctcataa accggattgg
     5101 ctgatttgac gagcagcttg ccgggtagtt tttggatttg tttgacaaac aggtgattat
     5161 cgatgcggat aacgtacagg ccgtcgcgcg gggtggtttc ggcgtgattc actaggatgt
     5221 tgtcgccgtg gttgagtacg ccctccatcg aatcgccctt tacggcgatg acggacagtt
     5281 tgtcaagctg gcgggtaacg tagttttcta tccaataacg cctgaatgcc atgcagaata
     5341 agggcttgtc gtcttctgcg ggatagccgt gccctgcggc ggcgtagacg tcgtaacgcg
     5401 ggataaagac gaactcgtct aagttgatgg ggttgccgag ggtatcggta gccgtaccgt
     5461 cggtatgggt acggacttcg accgcttggc ctttattcat gtatggcgag ccttggccgg
     5521 taagaagcca gtttaaatcg caaccggtta cttcttgaat tttaatcaga tagtctgctg
     5581 ttggaatggc accgtctttc cagacacggc taaaaccgga ggcagacata tcaatcttat
     5641 tgtagaagtc ggcgggtttg gcattgtccg gccacaaaga tttcaaacgc tctaaaaagt
     5701 ccatgattgt ccttttgctt agaatttcgc atttaagcaa aaaatacact tcttttgctt
     5761 aaaattaatt cgtcaaataa tcaaatagat ggctaatatt ttgctttttt gcgttatatt
     5821 tctaagcaaa agagttgcaa taaccttttt tgcttagtat tattcactca cttaatcaat
     5881 taacgaagta aatcacatta ctgaatggaa taagtcaatt atgcaaaaaa acgcaacgcc
     5941 gaaaaactgg caccgcgcgg atattgttgc tgctttgaaa aagaaaggct ggtcgcttcg
     6001 agcactttca atagaagcgg ggttgtcgcc gaatacgctt agaagcgcac tggccgcccc
     6061 ttatcttaag ggagaaagga ttattgccgc tgcaatcgga gtggaaccgg aagagatttg
     6121 gcccgaacgg tatgcagatc ggaatttaaa acctgttttc cccaaaagag tagttaatgg
     6181 ataagtggtt ttcgatttag ttccttaaat gctaaaggtt atacgcattt aagtaaaaca
     6241 caaaaagaaa gaaatagcaa aatgttgatt tcagcggttg agttggcggg cttgcaactt
     6301 ccaaagctgc cgaacagtag acaaggcatc gagtaccacg ccaaaaagaa taattggccg
     6361 tttgaagagg ttgtaggaca ggctcgaggc ggcaagctta agaaatattt ggtttccgcc
     6421 ctcccttcgg agattcaggc ggccatacag gaaaaacagg cggcaacatt gttggccaag
     6481 gtgccgatgt tgccggcaga agtaaaaaaa ccgcttcggc aaaataaaaa gatgcggcag
     6541 ttgggtttga taccgtgcga ggaaggtttg gcgcggttgg acgacaggca gacggaaacg
     6601 gcccacgcgc gttgtgcgat tgtcgcctat gtattgccgc tgcatgaatt ggcgggtatg
     6661 ccgattaaga aggcggtggc gtttgtggca gctgaagcgg cagcaggcag gttgccggag
     6721 gatgtggcca agctgattcc gttggcgaat gcgcggaaca acggcgaacg cagtttaagc
     6781 gaaccgactt tataccgatg ggtgcgggct taccgtgcgg catcggacag tatgagccgc
     6841 ctcttggcac ttgcgccggt gaaaacacgg gaaaagacgc cgctgctggc gattgactgg
     6901 ctgccttact tcttgatgtt ttatcagcgg ccgaacaagc cgacgatgat ggcggcggcg
     6961 aagaaactgg cgcaatggta tctggaacag ggaaaaatcg agcagatgcc gagctatgac
     7021 cagattcaaa cagtgatgaa acggttgccg gaacatatga aggagcgcgg caggcgcacg
     7081 gggtcggctt ataaggcttt gctgccttac attgatagag attggacggc gttgaaaccg
     7141 aacgatgtct gggtgggcga cggccacagc tttaaagcaa aaatccggca tccgatgggc
     7201 tatctgttta cgccggaagt gacaatgatt gtggacggtt gcagcggtgc ggtcgtgggt
     7261 tggagtgtgg cgctgtcgga aacggcggta gcggtggcgg atgctttgcg ccacgggatg
     7321 acgcatttcc cgccaccttt gggttattac tcggataacg gctcgggtga aacgggcgat
     7381 atgctggata aggaaacgac gggtattttg ccgcgtatcg gtatcgagca ttttacgggc
     7441 attccgggca atccgcaggg gcgcggcaag attgaacggc tgtggcagac cattacgatt
     7501 cctttggcca agcagtatgc gacttatcag ggcaaggatg cggatgccga aacattgaga
     7561 aaggtatcga atgcgctggc gagtgcgcag aaggtgcaga aaaagggcaa ggaactgagc
     7621 aaggcgcagg aagcggcgct gtcggcggca ccgacttggg cgcagtttat agcggatttg
     7681 gaagaagcgg tacgccgata caactttgaa cacgagcacc gcagcctgcc gaaaaatccg
     7741 gaagcgggac ggcattttac gccgatgaaa tattacgaat accggatgga aacggacggc
     7801 atgagggtga aaacggatgt attaagcccg ctggagttgg attttatgta ccgtccggag
     7861 gaagaacgca tccccgaccg cggagcggtg tctctgcaca ataatactta cttccaccaa
     7921 gaattgctgg attacagcgg tcagaaggta cgggtggctt acgacatcca cgatgccgac
     7981 catgtgattg tgaaggatat gcagggcaag gtgatttgca aagcggtatt caacggcaac
     8041 aaacgggcgg catttgcgga aacgcggatg gaacaactgg ccgaacgccg ccgcaaaggc
     8101 caggccaaac gtctgcaaga caagatggat ttaatcgagg cacaacgcca atcggccgcg
     8161 ccgattatcg agcagcagcc tgatttcggc gaatttttga agctggaaac ggaaagcggc
     8221 gggttggtag aggtggaaaa caggccgtct gaaagcggca ggggcaagaa gaagttacgc
     8281 gattttttat gggaagacgc cggttagtaa aacggcacat ctttgacact ttttaaggaa
     8341 aacgaaaaat gattgagaaa ttaagacaat ttctggaaac cagcggcatg agccaaaaca
     8401 aggcggccga ccgcatgggc gtatcgagaa gtgcattgtc gggctatttg aacggcaaat
     8461 acgacggcga tattgcgggg atggataaga aagccgcgct gtttcttgaa caggaaggcg
     8521 accgggcgga attgaaaaag ctggatattc cctatgttga aacgggcacg gccaagaaga
     8581 tgaaaggttg gctgggtttg gcggcatggc tggggcagct cggtattgtg tatggcggtg
     8641 cgggcttggg taaaaccacg gtattgaaac aatatgcggc cactaacccg ctggctttgt
     8701 tgattgagcc ggatacaggc tatacggcaa aggtgttgtt gcaggaaatc tgccatgcac
     8761 tggatttaag cgagagaggc aatatccatg agctgacgga gcggattatc aactgcctga
     8821 agcgtgataa gaagagccgc agcccgcgtg atgcacaccg tattttgctg attgacgagg
     8881 cggaacagtt accgacacgc gcactggaga gcctgcggcg gatacatgat aaatcgggcg
     8941 tggcggtggc gttggtgggt atgccaaagc tgctgctgaa tctgaagggg ccgaacagtg
     9001 aattcaagca gttgttttca agggtatcgg tcaagatgga attgggcgag atgctgccgg
     9061 aaacggattt aaaacagatt gcggcggcag tgttgaagac gaacgatgag gcggtattgc
     9121 agaaggtggt gaaaacggcc aaaggcaatg cgcggaaact gtcgaagctg ctgctgattg
     9181 tggactattt gttgcaggtt aaccctgatg ttgatttgga tgatgatgta atcgaacacg
     9241 cggaaaccta tttaatccac taaacctttg acagataagg caataatttt tttaccttgt
     9301 tattttgtta agttattgaa attaaaggaa aactaaaaat ggaaaatttg aaaatgcggc
     9361 atctgctcgc cataccggcg attgtgattg cggtaacggc gtgtgcgaac cgctgcaccg
     9421 agccggtgcc ggcgtggagc ctgtatcaga acgagaccag tcagcagcgg ctgatgcggg
     9481 aaaaacagaa tgcggctgac tttgcggtat tgcaggcaga aaaagcctat gaacggatga
     9541 gcgataaaga gcggatgaaa ggggtggttt atgaaaactg agacgaaaat ctgcaaaaaa
     9601 tgcggagaag agaggccgtt gttccgtttc aaaaaagacg atgcgtatgc gggcgggtat
     9661 aggcctgtat gttacgagtg tttaaacgcc agagcaaagg aactgaaggc agtccgagac
     9721 agtatggcgg aaagaaagat tatctctaaa ggcatcagca taaatggagg gaaaacgcat
     9781 atggaatttc ctgaagaacc tgaatttatg aaaaacaaat actggaccgt tacaaaaacg
     9841 gattatagaa gaccattaaa aggactttaa ccatgagtaa acggttggca cgacggaaga
     9901 tgccgaaaca cgaattgaaa cgggttaaag cgttcgccct gaaacaggcg gtcgagaaga
     9961 ttcgggcacg gtggggcgag aaagcgatac ggcttgcctc aacagacaac aaacagactg
    10021 attaaacaag gaataagaaa atgatgaatg aaatgaatgc ggtagcggct ttgttggtta
    10081 ttgccatatt gctggcggcg gtaatgactg tgttggtcga gatattggtc gagcaacggg
    10141 ttaaggaacg gctggggaaa ctgctggata aggcgtgccg accggatgac gggtggcagg
    10201 aatgataagg aaggaaatgt gatgaatatg gtactgacaa tttatttttg gttggtgggg
    10261 ctggtctgtt ttgcccgttc cgccaaagag acttattacg cggaatcttt atacggtctg
    10321 ctgactgggc tgttgggggt gatgtgttcg gcttttgtgg tgtcagtgtc ggtggcaagg
    10381 gtgatggggt ggttgaaatg aatcaattga aaatgacgat cgccaaacct gagacggaag
    10441 attttgaaga tgcctgggca tttatcagaa tgcttaattt ggtgacttat gatttgaacc
    10501 ctctgaaaac tgataccgac ggcgaatacg aatatttggc ggatgaagac aaatctgatg
    10561 tattagatgc cgttgtcgaa aaatttaacg aatgtagtct tgagtggatg ttgtcggcgc
    10621 tgcaagcact gatgtcgccg gagatgggga ttatcaacca agattcggat acgttggagt
    10681 tgcatcccaa actgaaaggc ggtacggaat gaaagtgcgc tgccctacct gcggtgcggt
    10741 gatgagctta gatgtattaa tcgcccatga tgatgcagcg aagctctgat tgccctgaca
    10801 ggcatttcag acgacctttt taaggcggta ttgcggtatc tgacgctgtt tcgccccgcc
    10861 gaaaaggatt taagttttaa ccgagtttca aagctcgtcg gcgagattgc gccgatgata
    10921 cgggacggaa agattgtccg caaccgcaaa acttatccgg ccccgcgcga ggcttggatt
    10981 tgggcggcaa cgcgatgcct tgaggcacgg gacgcgggaa aactgacacc gccactgacc
    11041 agccacggtt atttgttgga aaacattacg ttttggtcgc ctgaaaagac ggcgggaacg
    11101 gcggttttgc cctctcccca accatctcac acgagagagg tggcaagcac caaattgagg
    11161 agcgggttgg gcggcttgat ggagtggtca aatggaggaa aacaatagct ggctgaaaaa
    11221 agcaatcgcg cagggtttca tgatgctcgc cgccctaaac ctcaaaggcc gccctgcctc
    11281 ggcggatttg acggcagtcg ccgaactttg gttggtcata ctaagcggcc ggtcgtggca
    11341 gccggagcat gacgggatca ggatacaggc agcctttagg gctatcgcgg cgtcctcgtc
    11401 agagtggcca aaccctgccg accttatcaa gcacctgcca ccgggcgaag tcaggatggt
    11461 gccgaggctg gaaaagaagc actgcccgac ggaatacggc aaagagcagg ccgccgaact
    11521 gaaaaagatt gtcggcagat tgaaaaacgc accctgcatg aatagagatt ggatacacgg
    11581 gcaacgccac cggtcggtgg acgaatgtaa aaggatttat gccgaaaggc agaagggaaa
    11641 taaatagcca aatgagtacg ttttttgaca atcaggaatc cgacttaata aacgaatttc
    11701 tgacggaata ctgggaagag tttgaaaaat tctccgccaa caacggattt gatgacgatt
    11761 tattgaagca aatatcaatg gaattggaag gacataggaa ccattatgag taacttagac
    11821 atgaaacaat acaaacagga tgccaaaggc aatctcgtgc cgctggccaa tattaaagaa
    11881 atcgacctgc tgcgcgacga gctggtgcag gaaattgccg ccaaagcccg cgcggtacag
    11941 gataacttga tggcgttcaa acgcgaggcg atggacgata tcgcagcgtt tgtacagttg
    12001 agtgccgacc gctatgacgt atctgtcggc ggtaagaaag gcaatatcag cctgcacagc
    12061 tttgatggag cgtaccgcgt caaccttgcc atacaggaca cgttggtatt tgacgaaggt
    12121 ttgattgccg ccaaagccct gattgacgag tgtatcaacg aatggacgga aggcagtcgc
    12181 acggaattga aaacactgat taacgcggcc ttccaagtgg acaaagaagg caatatcagc
    12241 actgcccgcg tcctcggtct gcgccgcctg caaatcacgg atgaaaaatg gcaacgggcg
    12301 atggatgcgc tctccgacag tttgcaggtg catatcagca aaccgtttgt gcgggtatat
    12361 cagcgcggcg aggatgggga gtatcagtta atgaatttgg atgtggcgaa ggtgtaggaa
    12421 aaatgaacgc aaatataatg atttatttga ttgaagttaa tgatgggccg gttgcaaaag
    12481 cactgcgaag ttttcagcag gacaaggccg caatgcggca ggcgtggctt gattgggcgc
    12541 gagaacactt gccgtcagac gcgctaatgc gggaatggag cgatgggcaa gtggcagggt
    12601 ttgcgttccc ttcaggcata cccgatggat ggaaaaagcc caataagaac ggaatttgtt
    12661 ggccacggca aaacaaccca atcctcaaaa ccatgccact taacaaaaga tttaaacgcc
    12721 ccgaagagta tttggaagaa gttggcatca ccgcaccaac aatgattttt gaaaagaata
    12781 gcgacggaga gacttgggca tcttggggta tcggtaattt ctttaatcct gttcagttcg
    12841 tttgggcggg attggaggag gacgcgccaa agggcgtggt aactcctgat tatgcctacg
    12901 agcttagaga aggggctaag agaatcagaa acggctgtac gatgcagccg cccgaagatt
    12961 ttgattggca gaatctgtta ccgggctgcc gtgtcattcc tcggtatgag tgggattact
    13021 tggttgggaa atggcaggaa acacggaatg atgccgaaga acaggaggct taaatggcaa
    13081 aaatcattat tgaaatcgaa gacctgcccg agggcaccga cattaatttt aagggcgacc
    13141 ttccggcggc ggatgcaaaa gacaaaacag gtgcgcagca aacggctgtg ttaatcagca
    13201 agatgattca ggcggcgcag atgatggcat ccccatccgt aaacattagg ccgtctgaga
    13261 ctgcaaatcc ataacccgcg cggcacggtc tgccgcattt aaatctaaat aggagtcaaa
    13321 aagtgaataa atccgaatta atccaagcca tcgccgacga ggcggaattg agcaaacgcg
    13381 ctgcggcgga gtttgtcaat gcgtttgtca gcgtggtaac gcaagaactg aaagacggaa
    13441 acgacgttac gctggtcggc ttcggcacgt tccacgccgc ccaatccgcc gagcgtcagg
    13501 gacgcaatcc gaaaacgggc gaaccgctga ccatcgcggc gcgcaaaaca cccaagttcc
    13561 gctcgggcaa ggcgttaaaa gatgcggtaa acgtctgaaa ccccaaatcc aagcggattt
    13621 ttgaaaagtc cgcttcagtt tgaagtttta gcgacgagga aaaccatgat gaacacaaac
    13681 gaacagtaca aacgcaaggg gctgattgcg aaaatcaaaa tcgcgcaaag ccagcttggg
    13741 atggaagacg atgtttaccg tgcgatgctg gcgcgtgtgt gcggcaaaac atcctgcacg
    13801 cagatgaatc tagccgaatt gcaggcggtg gcggcggaaa tgaagcggat gggctttaag
    13861 cagaccgcac cgaagggcaa aggtatccgc ccgcatttga cacaggaccg tgccgcgctg
    13921 ttgaacaagc tggaagcgct gttgacggtg ggcgataaaa gctggcagta cgccgacggg
    13981 atggccaaaa ggatgttcgg caaggatttg gtacgatttt tgacacccga gcagctatac
    14041 aaactggtgc aggcgctgca aatccatatc aacaaagcga aaaaggcggc agagcaatga
    14101 tcagggtgga tgaagaagat tttgaggcgg tgcgccacct gctgcccgaa agtgtgctgg
    14161 cactgattac gatcatcggg ctgacagaaa cggtggagct ggtgaaaaac ttaggcggca
    14221 cgacctaccc gttgcggcag ggacgcacca aaggcagcga atcgcggctg gcttatttgg
    14281 aggaaatcgt cggcggtgcg gcgatggaaa aaatggtgga agcactggcc ccctgcgatt
    14341 tgtttattcc gaaatgcgag caggctttgc tggaattgcg tgaccgccat atccgccgcc
    14401 gcttcgatgc gcagacgggt aaaggcgtac cggcttatga ggcggtgaac gatttggctt
    14461 tggcgcacgc gctgaccgac cgccatatct ggcggattct gaagaagccg gataatgagt
    14521 gtgagcaagg cgggttgttt taaattataa tgccgtgggg ataacacggc attttttatg
    14581 ggaaatcaag atgaagacgt tttatgttct gatgttggcc gcaatgcttg cggcttgcgg
    14641 cggacaagaa aacaagcctg ctgccggcga aagtcaaacc gataatcggg cgcagcaata
    14701 tcagaatcag caaaaacaga ttgccgcacg tttggctgat gaagaatatt tcatcggtga
    14761 aaacaatttg gaacaaatcc gtctgcacgc caatttgaaa gagcgcgctc aaaagctgtt
    14821 gtctttattg gcgcaggccg acaaggaaag ccgtgtgtgg gttttgcccg gcgatgcagc
    14881 caaaatcaaa gaattcaatg cggctttcgc ggcggtggca aaatctgccg aggaatcttt
    14941 cggcggccct tttcttgcgg ataaggcggg gctttatcaa tgtaccgctg cggccaatgc
    15001 ggcttacgat tattttttcg cccggcagag aaaggatgcg ctgaccgaaa attaccgtca
    15061 acaatacacg gacaacatag ctgcctgcca agggcaaatc cgcaccccgc cagccgccca
    15121 agcgacagtg tatgcgcgta aaggtatcag gctgccgttg aataattgtt tgccggtatt
    15181 ggccggagat gaagaatttg atacctacac ctgcccgatg gaagtaaaat aggccgtctg
    15241 aaacccagcc cgagagcaaa agccctcggg ctttttgctg cctgtctgac atggggcagg
    15301 cgcggtgcca aataagcgga caggataatc aaggctcgat aacagactaa gcaggacgaa
    15361 tctatgagca agattattgt gctgaccgca ggccacagca acaccgaccc gggtgcggtc
    15421 aacggaagcg accgtgaggc ggacttggcg caggatatgc gcaatatcgt ggctgctatt
    15481 ttgcgcgatg actacggttt gactgttaaa accgacggca cgggcaaagg caatatgccg
    15541 ctgcgcgaag cggtcaagct gattcgcggc tcggatgtgg cgattgagtt tcacaccaac
    15601 gctgccgtca gcaaagcggc gacaggcatc gaagccttga gtaccgttaa aaacaaacgc
    15661 tggtgtcagg tgttgagcaa agccgttgcc aagaaaaccg gctggaaact gcgcggcgaa
    15721 gacggcttta aacccgacaa tgcgggccag cattcgcgcc tggcttatgc acaagccggc
    15781 ggcattgtgt ttgagccttt tttcatcagc aacgacactg atttggcctt gtttaagacg
    15841 actaaatggg gcatctgccg cgcgattgcg gacgcgattg cgatggaatt gggggcggca
    15901 agagtatgaa tattattggt aaattgaaag aagctgcttc ctattttctt acaaaattga
    15961 ttggagaaaa tcctagtaat gagcaggtaa accgcgcact tatacagatg ccaaatgttc
    16021 gtccgataca cacctatcca cgcccaaatt taagaaactc aggcgtggca gccgcgaaac
    16081 gcgcggcgcg caaacgcaag aatcgtcgtt aatcatggga caggttgcgt tttacgaaaa
    16141 gatgattggg ctgtggtcgg ccaaaagccg tgaggcaagc gaacaggcag acttggcggc
    16201 gtttgaattt gcggagggcg aactggccaa ttatcaggaa atgctgaaac ggcacctgca
    16261 aaccaaaagt gtggaatagc aatgcgtatt ttggatattt ttaaaaaccc agcgacaggc
    16321 aatgtgtcgc actcgaaact gtgggcaaac gttgcctgcg cggcggggac ggttaagttt
    16381 gtgatgctgc ccgacccgtc ggcggagatt tgggcggtgt atttgggcat tgtcggcggc
    16441 tatgcggtgg cgcgttcgtt ggtcagcgtc aaacgtcagg aggtcgagaa tgaatctcgt
    16501 gaaactgctg gcgaataact ggcaaccgat tgccatcatc gcgcttgtcg gcacgctctt
    16561 ggcggtgtcg caccatcaag gctacaagtc ggcttttgcg aagcagcagg cggtcattga
    16621 caagatggaa aaagacaaag cgcaagccct gctgttgtcg gctcaaaact acgcccgcga
    16681 gctggaacag gcgcgtgcgg aagctaaaaa atatgaagtc aaggcgcacg ccgtcggcat
    16741 ggctttggcg aaaaaacagg cggaagtcag ccgtctgaaa acggaaaata aaaaggaaat
    16801 cgaaaatgtc cttactcaag accgtaaaaa tgcaggcggc ggttgtattg acggctttgg
    16861 ccatcacggc ttgcagctct acaagcgcgc cctcggctac ggaaattaag gttgtcgaaa
    16921 aggcggtcat gccgacaccg cctgccgcgt tgatggtcgc gccggtgcgc ccgaatccgc
    16981 cgaaagacgg caagacggcc acgctgttgg aacacgccgc cgagtttggc ggctatgttg
    17041 ccgaacttga aaaccaaa
//
LOCUS       DAWKOG010000027        16830 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.027, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000027 DAWKOG010000000
VERSION     DAWKOG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16830
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.027"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            29..807
                     /locus_tag="WJK66_000696"
                     /pseudo
     CDS             29..807
                     /locus_tag="WJK66_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243629.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            848..1378
                     /locus_tag="WJK66_000697"
     CDS             848..1378
                     /locus_tag="WJK66_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEZ7578444.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGSTVLDDAVIEDEVMIGAGSLVPPRKRLAGGYLYIGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(1389..1528)
                     /locus_tag="WJK66_000698"
                     /pseudo
     CDS             complement(1389..1528)
                     /locus_tag="WJK66_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575226.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor"
     gene            complement(1536..3131)
                     /locus_tag="WJK66_000699"
     CDS             complement(1536..3131)
                     /locus_tag="WJK66_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEZ7578445.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVENI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIENPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPA
                     PKPRDATVRMVEPDEAKFSGFIFKIQANMDPKHRDRIAFLRVCSGRFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLTFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(3238..3633)
                     /gene="hisI"
                     /locus_tag="WJK66_000700"
     CDS             complement(3238..3633)
                     /gene="hisI"
                     /locus_tag="WJK66_000700"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEZ7578446.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRILMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGSAWETADAVLKDEKEIYGSTH"
     gene            complement(3664..4431)
                     /gene="hisF"
                     /locus_tag="WJK66_000701"
     CDS             complement(3664..4431)
                     /gene="hisF"
                     /locus_tag="WJK66_000701"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEZ7578447.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAVNPENTRWEIFTHGGRNPTGLDAVEW
                     AIEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGIFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(4444..5181)
                     /gene="hisA"
                     /locus_tag="WJK66_000702"
     CDS             complement(4444..5181)
                     /gene="hisA"
                     /locus_tag="WJK66_000702"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEZ7578448.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(5213..5851)
                     /gene="hisH"
                     /locus_tag="WJK66_000703"
     CDS             complement(5213..5851)
                     /gene="hisH"
                     /locus_tag="WJK66_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEZ7578449.1"
                     /translation="MQIAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPERVSR
                     ADKVIFPGQGAMPDCIAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLRDRQGCRLKVPHMGWNTVRQTQNHPLFKDIPQDTRFYFVHSYY
                     FAPEDPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(5982..7484)
                     /gene="pta"
                     /locus_tag="WJK66_000704"
     CDS             complement(5982..7484)
                     /gene="pta"
                     /locus_tag="WJK66_000704"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEZ7578450.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRVNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVGPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDIVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(7660..8718)
                     /gene="fbpC"
                     /locus_tag="WJK66_000705"
     CDS             complement(7660..8718)
                     /gene="fbpC"
                     /locus_tag="WJK66_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HEZ7578451.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(8739..10256)
                     /gene="fbpB"
                     /locus_tag="WJK66_000706"
     CDS             complement(8739..10256)
                     /gene="fbpB"
                     /locus_tag="WJK66_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HEZ7578452.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(10324..11319)
                     /gene="fbpA"
                     /locus_tag="WJK66_000707"
     CDS             complement(10324..11319)
                     /gene="fbpA"
                     /locus_tag="WJK66_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HEZ7578453.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALVTYSGAAVLKSSQNKDEAKKFVAFLASKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(11570..12040)
                     /locus_tag="WJK66_000708"
     CDS             complement(11570..12040)
                     /locus_tag="WJK66_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HEZ7578454.1"
                     /translation="MTDTDTQADRFEQMMWQAVDKLFEQHDGKLESMDGREQELVLIW
                     RAKADIGNGGILQFVCNWGFPAAEKTCSVLKKIGAIHSAMLIHRAADALRKEIRHLQS
                     EGKNLKEMWDITKHLDNKTASLPNSLDEQYWQDPDKLYLPGWQYYSGNPVQTLF"
     gene            complement(12098..13474)
                     /gene="argH"
                     /locus_tag="WJK66_000709"
     CDS             complement(12098..13474)
                     /gene="argH"
                     /locus_tag="WJK66_000709"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEZ7578455.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASISFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIRSLIQSLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRRRVNRMPLGAAALAGTTYPIRREITAKLLG
                     FEQICQNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADQAGVDLSELPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGTAPEQVRFQV
                     KRWREMLA"
     gene            complement(13493..14362)
                     /gene="galU"
                     /locus_tag="WJK66_000710"
     CDS             complement(13493..14362)
                     /gene="galU"
                     /locus_tag="WJK66_000710"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEZ7578456.1"
                     /translation="MKPIKKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQSAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYERSGNSILGV
                     ETVEPSQTGSYGIVETEQLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     NLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(14390..14989)
                     /gene="rdgB"
                     /locus_tag="WJK66_000711"
     CDS             complement(14390..14989)
                     /gene="rdgB"
                     /locus_tag="WJK66_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEZ7578457.1"
                     /translation="MSEKPEKIVLASGNVGKLEEFGNLFEPYGITVLPQSEFSIPECP
                     EPYSTFVENALAKARHVAGHSGLPALADDSGICAAALNGAPGIHSARYAGSNPKSDTA
                     NNLKLAAELAGKADKSCCYVCVLVFVRHKDDPRPIIAEGVWHGQWNGVPAGENGFGYD
                     PYFYLPGHGKTAAELDSEVKNRESHRAQALADLIRKLAL"
     gene            complement(14982..15215)
                     /locus_tag="WJK66_000712"
     CDS             complement(14982..15215)
                     /locus_tag="WJK66_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578458.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTAGSKAFAVAAFLFA
                     FGALFGQIGALALYLRHKSLHAAQSAAKENRYV"
     gene            complement(15327..15860)
                     /locus_tag="WJK66_000713"
     CDS             complement(15327..15860)
                     /locus_tag="WJK66_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEZ7578459.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESIERWNKQA"
     gene            complement(15962..16420)
                     /gene="nudB"
                     /locus_tag="WJK66_000714"
     CDS             complement(15962..16420)
                     /gene="nudB"
                     /locus_tag="WJK66_000714"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEZ7578460.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPKGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPVVLQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            16631..16707
                     /locus_tag="WJK66_000715"
     tRNA            16631..16707
                     /locus_tag="WJK66_000715"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16665..16667,aa:Pro,seq:ggg)
BASE COUNT         3482 a         4042 c         5117 g         4189 t
ORIGIN      
        1 cagataccga acaccaaaca tcaaatatat atatcaggat aaccaaggga cggcagaagc
       61 taggaatacc ggaataaaaa attcaaacgg aaaatatata gtctttattg attcggatga
      121 ttttattaat tgtcaggtct tgctggattt tcttagtaaa gatgatattg atatgccgga
      181 tgtggttttt taaatgcggt taaatatgat aagggaagtg tttcacattt tggcgaagat
      241 tatcagcctg aaaaaatact caatcaatcc aaagtcgaag ttttgaaagg attatgccga
      301 tttagaaaat ttccgggttc ggcgtgtaat aagattataa aaagggaatt gattatcaaa
      361 gaaaaactgt tttttgaaaa agaggtttat gccgaagata tcgaatggtc aatgagatta
      421 tttaatgcgg caacaacttt ttcttatttg gacggttgtt attactatta tcggcaggga
      481 agaaaagatt ctaaaacagg aactgtttcg gaaaaaagta taaagtcatt attatatatt
      541 ttggagaaaa atgcggaaat ggaatttaat agggatatat caagttatct ttattccttt
      601 ctttcctacg aatatctcgt tttgcttttt atcatgacga gtaaaaatat agagtgtgat
      661 gctgatataa aaagaagggc gtatcattta aggtttatgc tgttaaagtc caataaattg
      721 atatataagc tgatattccc gataatcaca ttattcgggg tcgatattac aggcagaatt
      781 ttaaaagtaa tcagagggaa tgtttaataa atcctttaac actatacatc ttaccgaagg
      841 aggaaaaatg aacgcaatcc gaactttcca aaaccgcacg cccgaaatcc acgaaagctg
      901 tatgatagac gaagcgtgcg tcgtcatcgg cgaagtgtcg cttgccgaag acgtttccgt
      961 gtggccgtgc gccgtgttgc gcggcgatgt gaacagcatc accgtcggcg cgcgcagcaa
     1021 tatacaggac ggcagcgtcc tgcacgtttc ccacaaaacc gccgccaaac ccgaaggatc
     1081 gccgctggtt atcggcgaag acgttaccgt ggggcacaaa gtgatgctgc acggctgccg
     1141 aatcggcaac cgcgtcttgg tcggtatggg cagcaccgtt ttggacgatg ctgtgattga
     1201 ggacgaagtg atgattggcg cgggcagcct cgttccgccg cgcaaacgct tggcgggcgg
     1261 ctacctttat atcggttcgc cggtcagaca agtgcgcgtg ctgaccgatg aggaaaaagc
     1321 ctttttgaaa tattccgccg cgcattatgt gaagctgtcg aaacagtacg ggatgtgaaa
     1381 tcacatcggc gttcttgcgt cagccccaaa ttcatgcgga cgggatgcat ccgataacgg
     1441 tatccgatgc gccttgattt tgaccgtctg cgtttgagtt gcaggcaaaa atgccgtctg
     1501 aagccctttt ttcgggttta gacggcattt tatcgttaca gtttgaccga atgttcgcgc
     1561 gtttcgtgga acacgatgtc cggccaacgt tcttgcgtga gtcccagatt cacgcggtta
     1621 ggggcgaggt aggcgaggtt gccgcccgcg tcgatggcga ggttgcccgc gttggctttt
     1681 tcaaattccg ccagtttttt cttgtcgtcg cacgataccc agcgcgccga ccagatggat
     1741 gcgctgtcga acacggcttc tacgccgtat tcgttggcca gccgcgaggt aacgacttca
     1801 aattgcaaca cgccgaccgc gcccaaaatc aaatccgcgc cgctcatcgg tttgaaaacc
     1861 tgtaccgcgc cttcttcgcc gagttgttgc aaaccttttt gcagttgctt gattttcagc
     1921 gggtttttga tgcggacgct gcggaacagt tcgggcgcga agaatgggat gccggtaaac
     1981 gtcagttgtt cgccttcgga gaagctgtcg ccgatttgga tgttgccgtg gtttgggata
     2041 ccgataatgt cgccggcgta ggcttcttca accagctcgc ggtcgtggga catgaaggtt
     2101 accacgctgg aggcggcgat ttcgcggttg atacgcaggt gtttcatctt cataccgcgc
     2161 tcgaatctgc cggagcagac gcgcaagaag gcgatacggt cgcggtgttt cggatccata
     2221 ttggcttgga ttttaaagat aaatccggaa aacttagcct cgtccggctc gaccatacgt
     2281 acggtcgcgt cgcgcggttt cggcgcgggc gcccagtcaa ttaatgaatt gaggatttcc
     2341 tgaataccga agttgttaat cgcagagccg aagaatacgg gcgtgagttc gccggcgagg
     2401 aattcgtcga gattaaactc gttcgatgct gcctgcacca attcgatttc gtcgcgcaac
     2461 tgttggattt ccaatggaaa gcgttgttcc aattcgggat tttctatgcc tttgatgatg
     2521 tcgaactcgt gcggcaggcg ttcgccgccc gcttcaaaga gatagatttc gtcgttcagg
     2581 atgtggtata cgcctttgaa gtttttgccc ataccgatcg gccaggtaac gggcgcgcag
     2641 cggatttgca ggatgttttc cacttcgtcc aagagttcca gcgaatcgcg cacttcgcgg
     2701 tcgtatttgt tcataaacgt aacaatcggc gtatcgcgca ggcggcagac gtttaagagc
     2761 ttgatggttt gcgcttccac gccttttgcc gcgtcgatga ccattaatgc gctgtccacg
     2821 gcggttaaaa cgcggtaggt gtcttcggag aagtcttggt gtcccggcgt gtccaagagg
     2881 ttgacggtgt ggtctttata gtcgaactgc atcacgcttg atgccacgga aatgccgcgc
     2941 tgcttctcga tgtccatcca gtcggaggtg gcgaatttgc cggttttctt gccttttacc
     3001 gtacccgcgc tttgaatcgc acctgaaaac agcaagagtt tttcagtcaa cgtggtttta
     3061 cctgcgtcag ggtgggagat gatggcaaac gtgcggcggc ggcgcacttg gtcgaggatt
     3121 tcttgggaca tggttttctt tgcaaaaagg ttcaggccgc ttttcagacg gcccggacag
     3181 tgtttgagac ggcgaaattg tacaaaaaaa tgcctgataa ttcaatgttg gaggcggtca
     3241 gtgcgtgctg ccgtaaatct ctttttcgtc tttcaggacg gcatcggcgg tttcccacgc
     3301 gctgccacgc cagactttgt aaaagcagct ttctcgcccg gtgtggcagg cgatgccgcc
     3361 gttttgggcg atgagcatca caatggcgtc gccgtcgcag tcgaggcgca gttcgcggac
     3421 tttttgcgtg tgtcccgact cttcgccctt catccattgt ttttggcgcg aacggctgta
     3481 atagtgggca aagccggttt cgacggtttt ttgcagggct tcggcgttca tccacgccac
     3541 cattaaaata cgtttggttt cggcatcttg ggcgatggcg caaaccaaac ctttttcgtc
     3601 aaatttgacg gcttcaagca ggtttttatc catatttcct ttcagacggc atggtcgagg
     3661 cggtcagagg cgcacttcga tgccggcttc gcgcatagcg cgtttggctt cgcggatggc
     3721 gatttccccg aaatggaaaa tgccggcggc aagtacggca tcggctttgc cttcggttat
     3781 gccttcaatc aggtgccgga cattgccgac cccgccggag gcgatgacgg gaatgtcgac
     3841 ggcttcggca acggcgcggg tcagcggcag gttgaaaccc tgtttcgtac cgtccctgtc
     3901 cataccggta agcaggattt cgcccgcgcc gcgtttttgc atttcgatcg cccattccac
     3961 cgcatccaaa ccggtcggat tgcgcccgcc gtgggtaaag atttcccagc gtgtgttttc
     4021 ggggttgacg gctttggcat cgacggcggc gacgatggct tgcgaaccga aaaatccggc
     4081 ggcttcgtca atcaggtcgg gacgggtaac ggcggcggtg ttgatgctga ctttgtccgc
     4141 gccggcattg agcaggcggc ggatgtcggc aacggtgcgt acgccgccgc cgacggtcag
     4201 ggggatgaag acttgtccgg caacctcttc gatgatgtgc aggatggtgt cgcggttgtc
     4261 ggatgaggcg gtgatgtcga ggaaggtcaa ttcgtccgcg ccttcgccgt tgtagcgttt
     4321 ggcggcttcg acagggtcgc ccgcgtcgcg caaaccgatg aagttcacgc ctttgacgac
     4381 gcgcccgtct tttacgtcga gacaggggat gatgcgtttt gccagtgcca taatcggatg
     4441 cctttagtcg agggaatctg ccagttgctg cgcttgggca aaatcgatgc tgccctcgta
     4501 aatcgcgcgg ccggtaatcg cgcctgctac gccatgtttt tcggcggcac acagggcgcg
     4561 gatgtcgtcc aagccggtca gtccgccgga ggcgatgacg ggaatgcgga cggcttgggc
     4621 gagtttgacc gtcgcgtcga tgttcacgcc gctcatcata ccgtcgcgcc cgatgtcggt
     4681 gtagatgatg ctgttgacgc cgtcgtcttc aaagcgtttt gccaaatcaa ttacatgatg
     4741 cccggttacg gttgcccagc cgtcgatggc ggccataccg tctttggcat ccagcccgac
     4801 aataatcctg ccggggaagg ctttgcacgc ctcgcgcacg aagtcggggt ttttgaccgc
     4861 cgccgtgccg ataatcacgt cgttcaggcc caaatccaaa tattgtccga tggttttcaa
     4921 atcgcgtatg ccgccgccga gctgtacggg gatgtctttg gcaacggcgg caaggatgtc
     4981 tttgatggcg ggcaggtttt gcggaacacc ggcaaacgcg ccgttcaaat ctaccagatg
     5041 caggcggcgc gcgccttgtt tgaaccagtg cagcgcggtt tcggcagggg agtcggaaaa
     5101 gacggtcgcc tcttccatca gcccttgttt caggcggacg cagcgtcctt ctttcaaatc
     5161 gatggcgggt atcagcagca tagtttttct ccttgtgggg ggcgtgtccg gcttaccagt
     5221 ttaaaaagtt tttcaacatc gtcagcccgg catcgtggct tttttcggtg tgaaattgtg
     5281 tggcgaatac gttgtctttg ccgacgatgc aggcaaacgg ggacgggtag tcgctttcgc
     5341 ccaatatggt ttcgggatct tcgggggcga aatagtagct gtggacgaag taaaaacgcg
     5401 tgtcttgggg aatatcttta aacagcgggt ggttttgggt ttggcgcacg gtgttccagc
     5461 ccatatgcgg gactttcaga cggcatccct gccggtcgcg gaggtcgcgc tcaaagcgtc
     5521 tgactttgcc gccgaaccag cccaagccgt cggtgtttcc ttcttcgctg tggtcgaata
     5581 aaagttgcgc gccgacgcag attccgaaaa agggtttgtt ttttaaggca tctttgactg
     5641 cctcgtccaa gccgccgcgc gtcagtgccg caatacagtc gggcatcgcg ccctgcccgg
     5701 gaaaaatgac tttgtcggcg cgggacacgc gttcggggtc gccgcttaaa aagatttcgg
     5761 tattttttcc ggcaagctgc cccgccgtcc ggacggattt caatacggaa tgcaggttgc
     5821 ccataccgta atcgataatg gcgatttgca tggcttcctc ctcttttttt gcaatatggc
     5881 tgcgatttta acaaacaaat gtgccgtgct gataaaaatg ccgtctgaaa acgggagtct
     5941 gtcttcagac ggcatagggt ttaaacccgg aaagccgttt gtcagccttc catttgtttt
     6001 gcctgaacgg cagttagggc gatggtgaac acgatgtctt ccaccagcgc gccgcgcgac
     6061 aggtcgttga ccggtttgcg caggccttgc agcagcgggc cgacgcttaa gacgttggca
     6121 ctgcgttgga cggctttata ggtgcagttg ccggtgttca ggtcggggaa gaccaaaacg
     6181 gttgcctgtc ctgccaccgg gctgcccgga gctttggatt tgcccacacc cggcacggtt
     6241 gccgcatcat attgcagcgg gccgtcgatg gcgaggtcgg ggcgtttttc ccgggcaagt
     6301 ttggttgctt cgatgacgat atcgacatcg gggccgctgc cggagttgac ggtggagtag
     6361 gaaatcatcg ccactttcgg gtcgatgccg aaggcttttg cggaatcggc agactggatg
     6421 gcgatgtcgg caagctgttg cgcggtcggg ttcggattaa ccgcgcagtc gccgaagacg
     6481 aggacttggt tgggcagcag cataaagaat acgctggaca cgaggcttgc gcccggtgcg
     6541 gttttaatca gttgcaaagc ggggcggatg gtgttggcgg tggtgtgaac cgcaccggat
     6601 accaaaccgt ccacatcatt ttgcgccatc atcatcgtac cgagtaccac ggtgtcttgc
     6661 agttgcttgc gcgcgtcttc gggtgtcagg cctttggatt tgcgcagttc gcacatcggt
     6721 ccgacgtatt gttcgaccaa tgaggcggga tcgatgattt ccaaagagtc gggcaggctg
     6781 ataccgcgtt ctttggcaac ggcttcgact tcttcgcgtt tggcaagcag gacgcagcgg
     6841 gcaatgcctt tttcgtggca gatggcggcg gcttggacgg tgcggggttc tgcgccttca
     6901 ggcaggacga tgcgtttgtc ggcttggcgg gcgaagtcga tcaggttgta gcggaattgc
     6961 gccggcgaca ggcgttttgc ttcgcggcct gccaatacgg atacgtcttt cagcgcgtcg
     7021 ctcgaaccga agaaggtcag gccggttttt tcggctgccg cttcggcaac ggaggctgcc
     7081 gcgccgtcca cgacaaaacc ttccaatacg cccggcgcgg cggcgaagaa ctgtttggca
     7141 aggttcaccc gatttgccag ttcgtcggca tcggcgttgt cggaacggac ggcgaagacg
     7201 gctgccgcgt caagggacaa tgccagttcg acgtttttgc ctgcgaggta gattttgtcg
     7261 gcatcgggcg cgatgccttc gatgacgagg ttggcggcat cgagtgcggc aactttgccg
     7321 accagtgcgt cgaaccagtc gtcgcttttg ccttgcgcga gcagggtttc ggcggttgcg
     7381 tcaacggctt ggaaaatttg tgcgtccagt gcttttgcaa aggcttgtgc ggcggcggag
     7441 gcgtccagtc cggcagatac gggtacgatg agtacttttg ccatgatata tcctttcgta
     7501 tgctgcggtg tgcggcatat gtggttggaa ggggcggcat ataggcagaa acggctgcct
     7561 gcgtgccgtg tttggcttga ggcgcgcagg ttgaatatag caaacaaatt ctgtttccaa
     7621 caagataaat atccgcaggc ttgtggatgc tgccgccttt cagagggtat ttccggggaa
     7681 gaacagggcg ggaccgtcca aatggaggac ggcggaaatg ccgtctgaca gggtgggggc
     7741 ggaagggagg ttgagcgtga ggacggtttg tccgacccgg agactgattt cggtatgccg
     7801 cgctttgggc gtggttttga gaaccacggc gtgaatggag gcggtgggtg cggaatgggg
     7861 gtgaaggctg aactgttccg gacggatgag cagtgtgccg cgtgtgcctg cgggtgcgcc
     7921 gctttgaacg ggcaggcggc ccaatccgca atcggcggtg ccgtcggcgt tgagcgcggc
     7981 ggggaacacg atgccttcgc cgataaacag ggcggcatca aggtcggcag gttgtcggta
     8041 caattcgtga gggcttgcag tttggaggat gcgcccctgt ttcatcacgg caatccggtc
     8101 ggcgtattgc agggcttctt cgcggtcgtg gctgacgaaa acggcagatt tgccgttggc
     8161 gcgcagggcg gcaatcatgt cttcgcgaat ctggcggcgc aactgttcgt ccagcgcgct
     8221 gaagggttcg tccaacaaaa tcagttcggg atcgggtgcg agggcgcggg cgagggcgac
     8281 gcgctgttgc tgtccgcccg aaagttcgtg cggatagcgt ccggcaagtt cggaaatgcc
     8341 ggtcaattcc aacatagctt cgatgcgctg ccgctcttgc gccgtcttgc ctttgccgtt
     8401 gcccagcccg taggcggtgt tgcggtaaac ggtcaggtgg gggaacagca caccttcctg
     8461 tacgacataa cccaaacggc gttcgcggac ggggaggttg gtatttttcg agaagatggt
     8521 tctgccggaa agcgaaattt cgccaaaatc gggttgttca aaaccggcaa ggcagcgtaa
     8581 aagggtggtt ttgccgcagc cggacgcgcc gacgataaag aggatttcgc ccgggtcgag
     8641 gctgagcgaa atgtcgttta aaaccggggt gttttgaaaa cttttggaca ggtgtccgat
     8701 gtgcagggcg gcggtcatgg cggtacttcc tcaagctgtt atttgaaggc gtatttcttc
     8761 agcaggaata cggggatgcc ggaaaataat accagcatca gcgcgtaagg ggtggcggcg
     8821 gcgtattgtg cgtctgatgt gtattcccaa acggcggtgg agagtgtgtg gacatcgtcg
     8881 gtggtcagca gcagggtggc ggtcagctct ttcatcagtt tgaggaagac gagtgcgaat
     8941 gcggcggtaa tgccgggcag gatggacggc agtaccaacg tcctgaaaat aaagaagtgt
     9001 ccgcgcccca atgttgcgcc gacctgttcc atcccttttg ggagttgttc caaggaagtc
     9061 ctcagggtgg tttgcgccat cggcaggtaa agcatgaaat aggcaaggat gacgacgata
     9121 aaggtttggt aaacggcagg ggtgtagttg atgctgaaat aaaccaagga tagggcgata
     9181 accaaaccgg ggacggcgtg cagtaaaaac ggcagcctgt ctatccaaac ggttaaaaaa
     9241 ttgcgatagc gaaccgatgc ccaaacaagg ggcaaggcac ataatatagt caaaatcgca
     9301 cctaaagccg atacgcttaa ggaacggata aaggcatcaa atacggatac gagcgcgaat
     9361 gtgccggaag tgccgaccat catccaatgt atcaatacgc caaaggggat aataatgccc
     9421 aaagtcaaca agctgcttaa aaaaacaatc gcgccgatct gaccgggcag tttgagggtt
     9481 ttgacgggat aaggacgggc aacgcctttg ccgctgtggt aaatcttggc tttgccgcga
     9541 aatatgcttt ctccaaatac gacgatgccg cacaccgcca ttaaaacagc ggaaagcagg
     9601 gcggcggtat tgttgttgta ggacatttcg tattcttgga aaatggcggt ggtaaaagtg
     9661 gggtagttca aaatggatac cgcgccaaat tcgaccaaca tatgcagggc aatcagtaac
     9721 acgctgctgc cgatggcggg tttgagctgg gggaggatgg cggaaaaaaa ggtttgcagg
     9781 cggcttttgc ccaaggacag gctgacttct tcgtaagaca ggctgatgcg tttgagtgcc
     9841 gcctcgacgg gcaggtaggc gagcgggaac gaggacaggc tcataatcat cactgtcccc
     9901 caaaagcctt cgacacggaa ggtcaggctg atccaggtga aacagctgac aaatgcgggg
     9961 atgcacaaag gcagggtgat tgccgtctga aaaaaggttt tgccgaagaa gcggtaacgt
    10021 tggaacaaaa gggcgcaggc aatgcccaaa acaatggaaa tcagggtaac gcccgccatc
    10081 atcgtcaagg tgttggagag caaatcccac atacgcgggc ggaacaacag ttcgacggcg
    10141 cggttgatgc cgacctgcca cgaacgcata gcgacatata aaaaaggcag ggtaagcggt
    10201 agggcaatca gtaggatgag gccggtaagc caaatgggta tttttttagg agacatagtg
    10261 tttttcatcg gcaaaacggg cggacagtat aaatgtccac ccgtttgaca atccgaaaac
    10321 ggcttatttc ataccggctt gctcaagcag ccgggtggcg tgttcttttt cggaaacagt
    10381 ggtggcggac acttggggtg cttccaactt ggcgatgggt tccaaattga aagtggatac
    10441 cacgtgcgga ttcaaaggat attcggcacg gacggcggtc agggcgcgct gtccttcctt
    10501 gctggcgagg aaggcgacga attttttcgc ctcatccttg ttttgggagg attttaacac
    10561 ggctgcgccg gaataggtaa cgagtgcgcc gggatctctg tggcggacga aattcaggcg
    10621 ggtgtggaca ttttgtacgc ctttttcacg cgcaaaagcg tgccagtagt agttgttgat
    10681 gagggcggca tcgatttcgc cgttttcaac cgcttgaagg gcgacggagt ttttagcgta
    10741 aggcttgccg tattctttca gacctttgag ccatttcaat gcggccgctt cgcctttcag
    10801 tttgacgatg gcgacaacct gttccaagaa cgcgccggaa gtgggggcgt aaccgatgcg
    10861 gtttttccat ttcggcgtgg cgtaattcag gacggatttt tccaaatctt tttcagacag
    10921 tttgcgggtg tcgtaaacga cgacgcgcga acgtccgctc agtgccaccc agtctttttt
    10981 ggcggcaacc ggcacgccct tgccgcgtgt ttcgttgatg gtggaggcgg gcaggggctc
    11041 taggaggttg gcggcggaaa gggtggcgag tgccgggatt tgttcggaat agaatacgtc
    11101 ggcggggctt cggctgcctt cttctttgat ttgaccggca agctggtcgc ctttggcgct
    11161 gttgagtttg actttgatgc cggtagcccg ggtaaaggca tctgcaacgg cttgtgctgc
    11221 ttctttgtgt tggccgttgt acacggtaat gtctgccagc gcgggggttg cggcggtcag
    11281 ggctgcggca agcagtgcgt atcggataga tgttttcata tcgattttct cctaatgaat
    11341 gagagtgtac accttgttaa aacataacgg tgtgtagtgt attccttctt ctttataaat
    11401 gcaaataatt attttttaaa tttgttatta tccgatccgg ttattgtttg ttctgacttg
    11461 tattttttcc gtgcatcgcg cccgtaaggc ggaagcggcg ggcaatgcgt ggcggaatgt
    11521 gggtaaaggc ggcattttga tttgtcggaa tgcttgagaa cccctctctt taaaacagcg
    11581 tctgaacagg gttgccggaa tagtattgcc atcccggcag atacagtttg tcggggtctt
    11641 gccaatattg ttcatccaga ctgttcggca gtgaggcggt tttgttgtcg agatgttttg
    11701 ttatatccca catttctttc aggtttttac cttccgattg gaggtggcgg atttcttttc
    11761 tcaatgcgtc tgccgcccga tggatcagca ttgcgctgtg tatcgcgccg atttttttca
    11821 gaacggaaca agtcttttcg gcggcgggaa aaccccaatt gcagacaaat tgcagtatgc
    11881 cgccgttgcc gatgtcggct ttcgcccgcc aaatcaaaac cagctcctgt tcccgcccgt
    11941 ccatgctttc gagtttgccg tcatgctgtt caaaaagttt gtccaccgcc tgccacatca
    12001 tctgttcgaa gcggtcggct tgggtatccg tatcggtcat cgtgttcttg ccttttaaaa
    12061 atgccgtctg aacatttctt cagacggcat ttgggggtta agccaacatt tcccgccagc
    12121 gtttgacttg gaaacggact tgttcaggcg cggtgccgcc caagtggttg cgcgcgttta
    12181 agctgccttc gggtgtcagc acgccgtaaa cgtcgtcggc aatcaaatcg ctgaaacctt
    12241 gtaagacttc gagcggcagt tcgctcaaat cgacgcccgc ttggtcggcg tggcgcacgg
    12301 cttgggcgac gacttcgtgg gcatcgcgga aaggcatgcc ttttttgacc agataatccg
    12361 ccaaatcggt ggcggtagcg aagccctgca tcacggcggc gcgcatattg tcgggtttga
    12421 cggttacgcc gcgcatcata tcggcgtaaa tccgcaacgt gtcgataagc gtgtcggcgg
    12481 tgtcgaacaa gggttctttg tcttcctgat tgtctttgtt gtacgccaag ggctgcgatt
    12541 tcatcagggt aatcaagccg ataaggtgtc cgatgactcg gccggatttg ccgcgcacga
    12601 gttcgggcac gtcgggattt ttcttctgcg gcatgatgga cgaacctgtg cagaaacggt
    12661 cggcgatgtc gataaagccg aaacgcggac tcatccacaa aatcaattct tcagacaggc
    12721 ggctcaggtg aaccataacc agcgaggcgg cggcggtgaa ctcgatggca aaatcgcggt
    12781 cggatacggc atcgagcgag ttttggcaga tttgttcaaa gcccaacagt ttggcggtaa
    12841 tttcgcgccg aatcggataa gtcgtaccgg caagggcggc tgcgccgagc ggcatacggt
    12901 tgacgcggcg gcggcagtcc gccatccgtt cgttatcgcg tccgagcatt tcgacgtagg
    12961 cgagcatatg gtgtccgaag ctgacgggct gggcgacttg caggtgggta aagcctggca
    13021 taacggtttc ggcgttttgt tccgccaaat ccagcaatgc cgtctgaagg ctttgaatca
    13081 ggcttcggat gacggtaatc tggtcgcgca gccaaaggcg gatgtcggtg gcgacttggt
    13141 cgttgcggct gcggccggtg tgcaggcgtt tgcccgcgtc gccgattttg tcggtcaggc
    13201 ggcgttcgat gttcatgtgg acatcttcca aatcggacga ccattcgatt ttgccgctgc
    13261 ggatttcttc gaggatttcc gccatgcccc gacggatgtc tgccaaatcg ccttcgtcca
    13321 acacgccggt ttctttcaac atttgcgcgt gggcgagcga gccttgaatg tcccattcgg
    13381 caagccgtcg gtcgaaactg atggaggcgg tgtattgttt gacgagttcg gaaacgggtt
    13441 cgttgaaacg tccggaccag gttttgtcgt gcataaggat tccttgatgg ggttattcgg
    13501 tgcggtattt ttccaaaagc cggcggaagg gttcgccggt ttcgggatgt ttcagaccgt
    13561 aggcgacggt ggcttcgagg tagcccagtt tgctgccgca gtcgtagcgc gtaccttcaa
    13621 aggggtgcgc caggacaaat tcgtgatcga gcagcttggc gatgccgtct gtaagctgga
    13681 tttcgttgcc cgcgccgcgc ggaagattgg ttaagaggtc gaaaatgcgc ggcgtgagaa
    13741 tgtagcgtcc gacgacggca aggttggagg gcgcgtcttc gggcttgggt ttttcgacaa
    13801 tgccggtaat gcgttggaac tgtttgagct gttcggtttc gacgatgccg tatgagccgg
    13861 tttgcgatgg ttcgacggtt tctacgccca aaatgctgtt gccgctgcgt tcgtacactt
    13921 cgaccatttg tttgagcgcg ccttttgggg catcgattaa gtcgtcagcc aaaatcacgg
    13981 caaagggttc gtcgccgatg gcggcgcggg cgcacaagac ggcgtgtccc aagcccagcg
    14041 cttccgcctg acggatgtag aggcaggtga tgttcggcgg caggatgttg cggacgtgtt
    14101 ccaacaattt gtctttatgg cgcatttcca actcggtttc gagttcgtat gccttgtcga
    14161 aatggtcttc gatgctgcgt ttgttgcgtc cggttacaaa caccatttcc gtgcagccgg
    14221 cttccacggc ttcttctacg gcggattgga tcagcggctt gtcgacgatg ggcagcattt
    14281 ctttcgggct tgccttggtg gcgggcagga agcgtgttcc catccctgcg acggggaaga
    14341 cggctttttt tatcggtttc attctttttc ctttgtattg ttttgatgtt taaagggcga
    14401 gtttgcgaat taaatcggct agtgcctgcg cgcggtggct ttcgcggttt ttgacctccg
    14461 aatccaattc ggcggcggtt ttgccgtgtc cgggcagata aaaatacggg tcgtaaccga
    14521 aaccgttttc accggcggga acgccgttcc attgtccgtg ccatacgcct tcggcaataa
    14581 tcggacgcgg gtcgtcttta tggcggacaa aaaccaatac gcagacgtag caacagcttt
    14641 tgtcggcttt gccggcaagt tcggcggcaa gtttcaggtt gttggcggta tcggatttgg
    14701 gattgctgcc ggcgtaacgt gcggaatgga tgcccggagc accgtttaag gcggcggcac
    14761 agatgccgct gtcgtcggcg agtgcgggca gcccgctgtg tccggcgacg tgccgggctt
    14821 ttgccagcgc attttcaaca aaggtggaat agggttcggg gcattcgggt atgctgaact
    14881 cggattgcgg caatacggta atgccgtatg gttcgaatag gttgccgaac tcttcgagct
    14941 tgccgacatt accgcttgcc aaaacgattt tttccggttt ttcagacata gcggttttcc
    15001 tttgcggcgg attgggcggc gtgtagggat ttgtgccgca aatagagggc gagtgcgccg
    15061 atttgtccga acagcgcgcc gaatgcgaaa aggaaggcgg cgacggcaaa ggctttgctg
    15121 ccggcggtca gcagataagc acccaagagg acgagcagca taaatgccag tgtgaacagg
    15181 gcaacagaca acagatagat ttttcggcgg ttcatggcgt tcggtcggaa acggtatgtt
    15241 cggattatat ccgattggga cggtattccc tagagcttgg aaaaatgccg tctgaagcgg
    15301 ctttcagacg gcagggcagg cggatgttat gcctgtttgt tccaacgttc gatggattct
    15361 ttaataactt ttttcgcttc ttccgcatcg ccccaggatt cgactttggt cgtaccggct
    15421 tttttcaggt ctttgtagtg gttgaagtgg aactcgattt gtttgatgag ctgctgcggc
    15481 aaatcggaca aagttttgta ggcgttgccg ttgttgcggt cgtcggcggg aacgcagacg
    15541 attttgtcgt ccacttcgcc gtcgtcaacg aatttcatca cgccgataac gcgcgcttcc
    15601 aagaatacgc cggttgccaa aggttgttcg gtaacgagca gcacgtccaa ttcgtcgccg
    15661 tcttcgtcca aagtttgggg aatgaagccg tagttggtcg gtttggcgaa gatggcgggt
    15721 tcgacgcggt cgagttggaa tgcggccagt ttgcggttcc actcgatttt gtggttgctg
    15781 ccggcgggga tttcgttgac gacgttgatg atgccgccgt ccacatcgcc gggggtcagg
    15841 atttggttga agtctgccat ttggtttcct ttgtttggga aagtttgaag tttgaaagta
    15901 tagcacaaac gtccggctga aaatgcaccc gatgccgtct gaaagggtgt acgggcgcgt
    15961 gttaccgttt gcccaaaaac ctgcccagtt ccagaatcgc gcgcctgttg gacggggaaa
    16021 acactttttc cgccgcttct tccaagccga accagccgta ggagacgtgt tcctcgggtt
    16081 gcaggacgac gggcgtatcg cgcgggattt cggcagagaa gaggtgttcg cggttttcaa
    16141 acacgccttt tggatagcgg tgccgccagt ggtggtagat ttcgtaaacc gtgctgtcgt
    16201 gccagtcttg aagctgcccg tccgccagca ggatgccggt ttcttcccaa acttcgcgcc
    16261 ttgccgtttg ggcgacggtt tcgcccggtt cgaggctgcc ggttaccgac tgccaaaatc
    16321 ctttcggatg cgtgcgttcg atgagcagga tgccgccgtc cccgctataa aggacgacca
    16381 gtgcggaaac ggggtatttg agcggttttg ccatcggcat ctttcggcgg gctgcggtaa
    16441 tgaaggggcg gattatagca aacgccgcac gttatggcgt ttatcctttt ccgtatcctt
    16501 ttttctgcac ggatgggacg cgccggtgtt tgccggtaaa ttttccgttg tgtcaaaaag
    16561 ataagggcgg ttgtgatttt aatgcttgcc aaagcgtcgg gcggaaacta taatccgaaa
    16621 cttatcgagt cggagtgtgg cgcagtctgg tagcgcactt gcatggggtg caaggggtcg
    16681 aaggttcgaa tcctttcact ccgaccaaaa attccgaaag ccgctttcaa aagcggcttt
    16741 tttgccgtcc gtatgattat gatgtagagt acgcggcgac agacattcaa atgccgtccg
    16801 aaaaccgttc agatggcatc tctttatctt
//
LOCUS       DAWKOG010000028        16536 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.028, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000028 DAWKOG010000000
VERSION     DAWKOG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16536
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.028"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(191..526)
                     /locus_tag="WJK66_000716"
     CDS             complement(191..526)
                     /locus_tag="WJK66_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEZ7578461.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFAQGL
                     PPGVGDFFR"
     gene            complement(607..2736)
                     /gene="dnaX"
                     /locus_tag="WJK66_000717"
     CDS             complement(607..2736)
                     /gene="dnaX"
                     /locus_tag="WJK66_000717"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEZ7578462.1"
                     /translation="MAYQVLARKWRPKTFADLVGQEHVVKALRNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCQSCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYEPAALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAENDVRQMIGAVDKQYLYELLTGIINQDGAALTAKAREMAACAV
                     GFDNALGELAILLQQLALIQAVPNALAHDDPDSDILHRLAQTIGGEQIQLYYQIAVHG
                     KRDLGLAPDEYAGFMMTLLRMLAFAPLAAASCDANAVIENTELQSPSVQTAEKEAAAK
                     KPQPRPEAETAQTSVQTASAAAMPSEGKTAEPVSNQENNDVPPWEDAPDEAQAAAGTA
                     QTSAKSIQTASEAETPPENQVSKNKAADNETNAPLSEVPSENPIQTTPNNEALETEAF
                     AHEAPAKPFNGYSFPDDDCPVEDNAEIPPPDWEHAAPADTAGGGADEEAEADGIGGNN
                     TPSAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARAT
                     ADKKRLDKIRDTLAQAYGLQLTLQTQDWQDESGRETPAMQDKRVQAEDRQKAQALLEA
                     DPAAQKILQAFGAQWQPESLELAANRP"
     misc_feature    complement(1508..1547)
                     /gene="dnaX"
                     /locus_tag="WJK66_000717"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            complement(2831..4807)
                     /gene="mutL"
                     /locus_tag="WJK66_000718"
     CDS             complement(2831..4807)
                     /gene="mutL"
                     /locus_tag="WJK66_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEZ7578463.1"
                     /translation="MSRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAIE
                     VELAGGGIRLIRVSDNGSGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQKDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSENTEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPAQSLHERIAAIVGDDFQT
                     ASLEIDSGNGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSQQVHQLVFHTLNKALADTRADLTE
                     SVGNAGEVLHEITGVTPAPMPSENDSENLFNRASDYPTGNKPDTRNAFGASGKTAPMP
                     YQAARAPQQRSLSLRESRAAMNTYAELYKKTDDIDLELSRFEQARFGNMPSETPAPQT
                     DTPLSDGIPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGRLQSQRLLIPVTFAASHEECAALADYAETLAGFGLELSDMGGNTLAVRAAPAML
                     GKSDVVSLARDVLGELAQVGSSQTIEEHENRILATMSCHGSIRAGRRLTLPEMNVLLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            4951..5136
                     /locus_tag="WJK66_000719"
     CDS             4951..5136
                     /locus_tag="WJK66_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEZ7578464.1"
                     /translation="MYHYQSDATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            5147..5815
                     /locus_tag="WJK66_000720"
     CDS             5147..5815
                     /locus_tag="WJK66_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEZ7578465.1"
                     /translation="MTTHLSNVAPDLQNYLNSIGEPEHPVLTRLREKTEHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEVGVFTGYSSTALALALPEHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIVQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDPRIVPITLPVGDGLTL
                     LLKK"
     gene            5815..6531
                     /locus_tag="WJK66_000721"
     CDS             5815..6531
                     /locus_tag="WJK66_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HEZ7578466.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRLEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGTLVRLSNEVETLNGKVKALEHAKTHPSSRAYVQKLDDRKLKEH
                     YLNTEGGSASAHTVETAQNLYNQALKHYKSGRFSAAASLLKGADGGDGGSIAQRSMYL
                     LLQSRARMGNCESVIEIGGRYANRFKDSPTAPEAMFKIGECQYRLQQKDIARATWLSL
                     IQTYPGSPAAKRAAAAIRKR"
     gene            6579..7064
                     /gene="purE"
                     /locus_tag="WJK66_000722"
     CDS             6579..7064
                     /gene="purE"
                     /locus_tag="WJK66_000722"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEZ7578467.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFQYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANENPELAQKLADFRAKQEQTVLNMELEQ
                     I"
     gene            complement(7247..8701)
                     /locus_tag="WJK66_000723"
     CDS             complement(7247..8701)
                     /locus_tag="WJK66_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HEZ7578468.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHVRQRSLSKLPALLEQLEENLTKLGVKVHWAETPAEACEII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(8698..9399)
                     /locus_tag="WJK66_000724"
     CDS             complement(8698..9399)
                     /locus_tag="WJK66_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEZ7578469.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREMGVSWGSEVERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALAGSNIE
                     PIVFEREIDTWKTEFFTNINAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPTDLEENA"
     gene            complement(9396..10175)
                     /locus_tag="WJK66_000725"
     CDS             complement(9396..10175)
                     /locus_tag="WJK66_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEZ7578470.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAITLIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVAKAQLDLFPENWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAKRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPNMPRPKHIASFLLERTGGKA"
     gene            10459..11865
                     /locus_tag="WJK66_000726"
     CDS             10459..11865
                     /locus_tag="WJK66_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ7578471.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADL
                     NESPLNMQLAVISYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRVYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDIKGSNISLDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSPLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLAISMGIVVGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FKRLHVSDGANLTRNTPS"
     gene            11977..13503
                     /locus_tag="WJK66_000727"
     CDS             11977..13503
                     /locus_tag="WJK66_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEZ7578472.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVFRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQTG
                     RIDWQSVQTRLISDDPAKGLDRDRRKSPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIESLL
                     "
     gene            13711..14244
                     /locus_tag="WJK66_000728"
     CDS             13711..14244
                     /locus_tag="WJK66_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ7578473.1"
                     /translation="MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
     gene            complement(14287..15588)
                     /gene="aroA"
                     /locus_tag="WJK66_000729"
     CDS             complement(14287..15588)
                     /gene="aroA"
                     /locus_tag="WJK66_000729"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEZ7578474.1"
                     /translation="MTESLRLPAASLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVEIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYRLHGVARMHERPIGDLADALRIAGADVEYLGKEHYPPLHIGERQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVIN
                     EGYRVFKIPADAHYHAPEHLHVEGDASSASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FACELEKIGADVVWGENFVEVSRPKERAVQAFDLDANHIPDAAMTLAVVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVAEEAEAIHITPPETPTPDAVIDTYDDHRM
                     AMCFSLVSLLDVPVVINDPKCTHKTFPTYFDVFSSLTETAE"
     gene            15980..16456
                     /gene="greA"
                     /locus_tag="WJK66_000730"
     CDS             15980..16456
                     /gene="greA"
                     /locus_tag="WJK66_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEZ7578475.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
BASE COUNT         3480 a         4473 c         4602 g         3981 t
ORIGIN      
        1 ctattcttgc cgtttttgcg ttcttatcat tctcactgtt tttaccgttc acgccgaatc
       61 ctgcaaactg atgccgtcat tcccgcgaag gcgggaatcc agacccgtcg gcacggaaat
      121 ttatcgagta aaacggtttc ttagattcta cgttctagat tcccgcctgc gtgggaatga
      181 cggtcgggga tcagcggaag aagtcgccca ctccgggggg gagtccttgg gcgaatgcgc
      241 ccattgtttt gtttgcggtt tcttcggctt tgccacgggc ggatttgagg gcggcgagga
      301 tgaggtcttc aagcatttct ttgtcgtcgg cggcttcttg aatcaaatcg gggctgatgt
      361 cgattttgcg tacttcgtgc gcgcaggtca ttgtgatttt gaccaagccg ttgcctgctt
      421 cgccttcgat ttcggtttcg gcaagtttgg cttgcgcttt tttcatattt tcctgcattt
      481 gctgcgcctg tttcatcagg ccgcctaatc cggcttttcc gaacatactg aatactcctg
      541 tttgtgttta aatgaatgag ggaaacggca atgccgtccg aagggttcgg gcggcattat
      601 atctgtttat ggccggtttg ccgccaattc cagtgattcg ggctgccatt gcgcgccgaa
      661 tgcttggagg attttttgtg cggcggggtc ggcttcgagc aatgcttgtg ctttttgcct
      721 gtcctcggct tggacgcgct tgtcctgcat cgcgggggtt tctctgccgg attcgtcctg
      781 ccagtcttgg gtttgcaggg tgagttgcag cccgtaggct tgggcaaggg tgtcgcggat
      841 tttgtcgaga cgttttttgt cggcggtggc gcgtgcttcg gcggtcattg ccaaaaccat
      901 caggccggtg tcgggatggt attccgtcca cgcggagtgt tgcgccggca tttgcgccgc
      961 gccgagtttg cgggcgaaat gccggacgat ggctgcccag ttttcggtgg aaaactcggg
     1021 cggtggggcg gacggtgtgt tgtttccgcc gatgccgtct gcttctgctt cttcgtccgc
     1081 gcctccgcct gcggtatcgg caggggcggc gtgttcccaa tcgggcggcg ggatttccgc
     1141 gttgtcttct acggggcagt catcatccgg aaagctgtaa ccgttgaaag gttttgcagg
     1201 agcttcgtgc gcaaatgctt ccgtttcaag ggcttcatta ttcggtgttg tctgaatggg
     1261 gttttcagac ggcacttcgg acaagggggc attggtttcg ttgtcggctg ccttgttctt
     1321 ggaaacttgg ttttcgggcg gcgtttcggc ttcggatgcc gtctgaatgc tttttgccga
     1381 cgtttgcgcc gtgccggctg cggcttgggc ttcgtctggc gcgtcttccc aaggcggaac
     1441 atcgttgttt tcttgattgg aaacgggttc ggcggttttg ccttcagacg gcattgccgc
     1501 tgcggatgcc gtctgaacgg atgtttgggc ggtttccgct tcagggcgcg gttggggctt
     1561 ttttgcggcg gcttcctttt cggcggtttg taccgatggg gattgaagtt cggtattttc
     1621 aatcacggcg tttgcatcac acgaagctgc cgccaagggc gcaaacgcca gcatacgcag
     1681 cagggtcatc ataaagccgg cgtattcgtc gggggcgagg cccaagtcgc gtttgccgtg
     1741 gacggcgatt tggtagtaaa gctggatttg ttcgccacct atggtttggg cgaggcggtg
     1801 caaaatatcg gaatcggggt cgtcgtgcgc caaggcattc ggcactgcct gtatcagggc
     1861 gagttgttgc agcagtatgg cgagttcgcc caaggcgttg tcaaagccga cggcacacgc
     1921 cgccatttcc cgcgctttgg cggtcagggc tgcgccgtct tggttgatga tgcctgtcag
     1981 cagttcgtaa aggtattgtt tgtcaaccgc gccgatcatt tggcggacat cgttttcggc
     2041 aactttgccc gaacccaggg cgatggcttg gtcgagcagg cttaaggcat cgcgcatcga
     2101 tccggcggcg gcgcgtccca aaagttgcag ggcggcgggt tcgtaggcga ttttttcgct
     2161 gtcgaggacg tgggcgaggt ggtcggcaac ctgctgcgcg gtcatattgc gcaagacgaa
     2221 ttgcaggcag cggcttaata cggtaacggg gactttgtgc ggatcggtcg tggcaaggat
     2281 gaatttgacg tgttcgggcg gctcttccag cgttttgagc atagcgttga acgcgctttt
     2341 ggaaagcata tgcacttcgt cgatgatata gactttgtat ttgccggcag tcggcgcgta
     2401 ttgggcgttt tccaagactt cgcggatgtt gtcgatgcct gtgttggagg cggcgtcgat
     2461 ttcgagcagg tcgacatagc gtccggcatc gatttgggtg cagctttggc acacgccgca
     2521 aggttcgccg tgttgcgcgt tttcgcagtt gaggcttttg gcaaggatgc gggcgatggt
     2581 ggttttacct acgccgcgcg taccggtcag caggtaggcg tggtgcagcc tgccttcgtc
     2641 cagcgcgttg cgcaaggctt tgacgacgtg ttcctgaccg actaagtcgg caaaggtttt
     2701 cggacgccat tttcgggcga gaacttgata ggccatgttt ttctcttggt ttcggtcgtg
     2761 atgtttctgt cggtgcttcg gaatgccgtc tgaacggcgg tctcgggcgg cgtattctag
     2821 cactttcggc ttattgtccg cgcaagaaca gtgcgtccaa ttctttcaaa gtcagtttga
     2881 cccacgtcgg cctgccgtgg ttgcactggt tgctgcgcgg cgtgttttcc atatcgcgca
     2941 gaaggacgtt catttcgggc agggtaagcc ggcggccggc gcggatcgag ccgtggcagg
     3001 acatggtggc gaggatgcgg ttttcgtgtt cctcgatggt ttggctgctg ccgacttggg
     3061 cgagttcgcc caatacgtcg cgggcgaggg agacgacatc ggatttgccc aacattgcgg
     3121 gtgcggcgcg gacggcgagg gtgttgccgc ccatatcgga taattccagc ccgaagcctg
     3181 ccagcgtttc ggcataatcg gcaagggcgg cgcattcttc gtgggacgcg gcaaaggtta
     3241 cgggaataag caggcgttgg ctttgcagcc tgccgttttc ctgacgctgg cgtttcattt
     3301 tctcgtagtt gacgcgttcg gcagcggcgt gcatatcgat gagcagcagg ctgtcttcgg
     3361 cttgggcaag aatgtagatg ccaagtaatt gggcaatggc gaaaccgagc ggcggcagtt
     3421 cggattggga ggggatgccg tctgaaagcg gcgtatctgt ttggggagca ggcgtttcag
     3481 acggcatatt gccgaaacgt gcctgctcga atcggcttaa ttcaaggtcg atgtcgtcgg
     3541 tttttttgta aagctcggca taggtgttca ttgccgcgcg gctttcgcgc agggacaggc
     3601 tgcgttgttg cggcgcacgc gcggcttggt agggcatagg cgcggttttg cctgatgcac
     3661 caaaggcatt gcgcgtatcg ggtttgttgc ctgtcgggta gtcggatgcg cgattaaaca
     3721 gattttcgct gtcgttttca gacggcattg gtgcaggcgt aactcccgtg atttcgtgca
     3781 acacttcgcc cgcgttgccg acgctttcgg tcaggtcggc gcgcgtgtcg gcaagggctt
     3841 tgttgagcgt gtggaacacg agttggtgta cctgctgact gtcgcggaag cggatttcgg
     3901 ttttggtcgg gtggacgttg acatctacgg cttcgggcgg caggtcgaga aagaggacga
     3961 aagcaggtgt cagcgcgttg tgcaatacgt cgcggtatgc ctgcttgaca gcatggagca
     4021 tgactttgtc gcggacaaaa cgatggttga caaagcagta ttgtttgtcg gttttgcctt
     4081 tggcgaaagt cggcttggca atcgcgccat agagccgcaa tgcgccattg ccgctgtcga
     4141 tttccaagga tgccgtctga aagtcatcgc cgacaatggc ggcaatccgt tcatgcaggc
     4201 tttgtgcagg gagtttgaac acttgtttgc cgtcgcgttt gagtgagaag gcgatgtgcg
     4261 gatgcgccag agcaagtcgt tcgagcatgg tggcgcagtg ggcgtattcg gtgttttccg
     4321 atttgaggaa cttgcgccgc gcgggggtat tgaagaagag ttcggcagct tcgatggtgg
     4381 tgccgacggg gtgggcggct gccgtagggc tgctgagttt gccgtcttcg gctttgactt
     4441 gggtcgcgtg cgaactgtct ttttgacggc tggtcagggt caggcggctg acggaggcga
     4501 tgcttgccaa gccttcgccg cgaaagccca tgctggcaac gtgttccaaa tcgtttaagg
     4561 ttttgatttt gctggtggcg tggcggtgga gcgcgagttc gatgtcgtcc ggatggatac
     4621 cgctgccgtt gtcgctgacg cgaatcaggc ggatgccgcc gcccgccagt tcgacttcaa
     4681 ttgctgttgc gcctgcatcg atactgtttt caacgatttc tttcagggcg ttggcagggc
     4741 gttcgaccac ttcgccggca gcgatttggt tgacaagatg gtcgggcagg gcggcgattc
     4801 gggacataag gcgggcttcc gttgcaaaaa acgtcttatt ctataataaa accccttatc
     4861 tttctgcccg tattcttgat aaaagccgtt tatcccgttt ggaaaatacc agtataatca
     4921 cgccatattc cgtaaaattg gagcacaaag atgtatcact accaatccga tgccacacaa
     4981 ttcctcaacc gcctgattga agaaaaacct gagttggaac agcagcgttt ggaaaacagg
     5041 gggcttttgt gggatgtcga actcaatccc gaagaacagg aaaacttcga ggcggcaaaa
     5101 gtagcgaaaa aaccttatac ctattaccaa gactgaaccc gccgaaatga cgacccatct
     5161 gtccaatgtc gcaccggacc tgcaaaacta tctgaacagc atcggcgaac ccgaacatcc
     5221 cgttttgacg cggctgcgtg agaagaccga gcatcaccgt atgggcaaaa tggcgattgc
     5281 gcgcgaacag gcggcggttt tggtttggct ggcaaagctg attcgcgcgg aaaaatatct
     5341 ggaagtcggc gtgtttaccg gatacagcag caccgcgctt gcattggcgt tgccagaaca
     5401 cgggcggatt accgcctgcg acatcaatgt aacctttacc gatacggcgc gtcaggtttg
     5461 gaacgaggca ggtgtggcac ataaaatcag cctgcacctg caacccgcat tgctgacatt
     5521 ggatgatttg attgtacagg gtgaagccgg aagctacgat ttggcactga tagacgcaga
     5581 caaaccgccc acgccgcaat atttcgagcg ttgcctcaaa ctcgtccggc aaggcggcat
     5641 cattgccatt gacaatattt tgttgaacgg aagggtgatg cgcgaagcgg cttccgacgc
     5701 gccgcccagt gtcggcatcc tcaaagattt caatcaaaac ctgccgaacg acccgcgcat
     5761 cgtccccatc accctgcccg tcggcgacgg tttgaccctg cttctgaaaa aataatgaag
     5821 accaaattac cgctttttat catttggctg tccgtatccg ccgcctgttc ttcccctgtt
     5881 tcccgcaata ttcaggatat gcggctcgaa ccgcaggcag aggcaggtag ttcggacgct
     5941 attccctatc ccgttcccac tctgcaagac cgtttggatt atctggaagg cacactcgtc
     6001 cgcctgtcga acgaagtgga aaccttaaac ggcaaagtca aagcactgga gcatgcgaaa
     6061 acacaccctt ccagcagggc atacgtccaa aaactcgacg accgcaagtt gaaagagcat
     6121 tacctcaata ccgaaggcgg cagcgcatcc gcacataccg tcgaaaccgc acaaaacctc
     6181 tacaatcagg cactcaaaca ctataaaagc ggcaggtttt ctgccgctgc ctccctgttg
     6241 aaaggcgcgg acggaggcga cggcggcagc atcgcgcaac gcagtatgta cctgttgctg
     6301 caaagcaggg cgcgtatggg caactgcgaa tccgtcatcg aaatcggagg gcgttacgcc
     6361 aaccgtttca aagacagccc aaccgcgccc gaagccatgt tcaaaatcgg cgaatgccaa
     6421 tacaggcttc agcaaaaaga cattgcaagg gcgacttggc tcagcctgat acagacctat
     6481 cccggcagcc cggcggcaaa acgcgccgcc gcagccatac gcaaacgata gtttttccat
     6541 ttttccgctt tttaccccat ccgcaccaag gaggatttat gatacaaatc ggcatcatca
     6601 tgggcagcaa cagcgattgg cccgttatgc ggcaggcagc gcagtttctt gaagagttcg
     6661 gcgtagaata tgaggcgcgc gttgtttccg cacaccgcac cccggatttg atgttccaat
     6721 acgccgaaac cgcacgggcg cgcggcatca aagccatcat cgcgggcgcg ggcggtgcgg
     6781 cgcatttacc cggtatggtt gccgccaaga ccaccgtccc cgttttgggc gtacccgtcc
     6841 ccagcaaata cctgcgcggc gaagattcgc ttttatcgat tgtacaaatg cccaaaggcg
     6901 tacccgtcgc cacattcgcc atcggcgagg caggcgcggc aaatgccgca ttgttcgccg
     6961 tttccatgct ggccaacgaa aaccccgaac tggcgcaaaa actggcagac ttccgagcca
     7021 aacaagaaca gactgttttg aatatggaat tggaacagat ttaaaacccg ccgtccgcga
     7081 atatatagtg gattaaattt aaaccagtac ggcgttacct cgccttgccg tactatttgt
     7141 actgtctgcg gcttcgtctc cttgtcctga tttttgttaa tccactatac aaatgccgtc
     7201 tgaagccctg tttcgggttt cagacggcat ttttgcgaac aactttttat gcctgttctt
     7261 tttggcgcat tttttctgcc atcagttcgt gcagggtttt cttcgccggt ttcatcggca
     7321 cgcggttttg cgtccaaccc aactgtttgc gcggggtcag gttgcggaac ttggtggctg
     7381 cccaaccgaa ggcgcggtag gttttgctgc cgctgaaaat accgttgaat gtgcgccacg
     7441 ccatttgttc gccgaaggta tgcgatgcgc cttgccctcg gatggggtgc ggtacggttt
     7501 cggtcggcga acgttgcgct tcaacgcgca aacgctgcat ttgttcggta atcgggatgc
     7561 gtaccggaca gacttccaca cacgcaccgc acatggtaca ggcggtcggc agatcgcggg
     7621 tggcatcaag acccaacagg tgaggggaaa taatctcgcc aatcgggccg ggataggttg
     7681 tgccgtatgc cgcgccgccg atgcgggtat aaaccgggca atggttcata cacgcgccgc
     7741 aacggataca ttgcagggtg cggcgcattt ggtcttcggc ataagcctgg ctgcggccgt
     7801 tgtcgagcag aaccaagtgc atttcttgcg gaccgtctaa ttcctcactg cggcgcggac
     7861 cggtaatcat gttgaaataa gtggtaatgt tctgaccgat ggcagaacgc ggcagcagac
     7921 tgtacaaggg tggaatatcc gacagcttgg caactacttt ttcaatgccg gtaatggcga
     7981 tatgcacggg cggaacggtg gtactcaagc gaccgttgcc ttcgttttcc accagacaca
     8041 gcgtacctgt ttcagcaacg gcgaagttca cgccactcaa accgacatcg gcagtgctgt
     8101 aaatatcgcg cagtgcttta cgggcgaagc cggtcagttg gtctacgtcg tccgttaacg
     8161 gtgtgccgag gttttggtgg aacagttcgc taacctgttc tttggtttta tggattgcgg
     8221 gcatcacgat atgggtcggt ttttcacctg ccatttggac gatgaactcg cccaagtcgc
     8281 tttccaccgc tttaatgcct tttgcttcaa gataatggtt cagctcgatt tcctcgctga
     8341 ccatcgattt gcctttgacc atcagcttgc cgtttttggc tgtgatgatg tcgtggatga
     8401 tttcgcaggc ttcggccggt gtttccgccc agtgtacttt tacgcccaac ttggtcaggt
     8461 tttcttccag ctgctccagc agggcaggca atttagacaa tgagcgctga cggacgtgtt
     8521 cgcacaaatc gcgcaagctt tgcagctctt cttcgtcggt caaaacagct ttgcgtttgg
     8581 tcatcagcat atccatcgcg gtacgcaggc ttttacgcaa aggcttgtct tgaagggaaa
     8641 ttgcggcgtt ttgcttgaaa gtttccggct tcatgtgaaa tttgatggtt tgcgtagtca
     8701 tgcgttttcc tccaaatcgg taggggaaat gtggtcgggc aggatggcga ggataaccaa
     8761 atcacgcgga ccgtgcgcgc cgtaagcaag cgtcagttgg atgtctgcgg ttttggacgg
     8821 accggaaatc aggaatacat tggtcggcat accgttttcc accagttttt cgccttcgac
     8881 ggcattatga aactcgttgt acatcttgga cgtatcgaac aggcagaaat gcacgggcgg
     8941 aacgagactc aaagtacgcg gttcttcggg gctggaaaac agcatcagcg tgccggtgcg
     9001 ggcgatgccg cattgcgcgc cgctgaagcc cgcattgatg ttcgtgaaaa actcggtttt
     9061 ccaagtatcg atttcgcgct cgaagacaat cggttcgata ttgctgcccg ccaatgcggc
     9121 acgggcaatt tgtccgtgtt cggtcgccaa gggcagcagg atgtttttca aacccttgcc
     9181 ttctgccgct tcgcggaaaa cctgcatcca attgcttttc gtcacccaat aaatttcggt
     9241 tttgaccgca cgcatagcgg cagcccaatg tttcagacgc tcaacttcgc tgccccaaga
     9301 aacacccatt tcacggtaat aatcaaaaac cgcaggttct tccatcggca atgcgtcggc
     9361 ttttttcagt tttgccaaaa tattttcgcg cgcgctcatg ctttgcctcc ggtacgttcc
     9421 aacaagaagg atgcgatatg tttcggacgc ggcatattcg gttcgtcctt agcaattttg
     9481 ccgccaatgt tcatcatgca gccgcagtcc gcgctgatga tttcggtcgc gccggtttct
     9541 ttcagcgcgg cgactttgtc tgttaccatt gcgccggaaa tatcggcttg tttgacggag
     9601 aatgtgccgc cgaagccgca acattcgctt tcgtggtcgt ggacgatgcg ttcgacgttt
     9661 tccataccgt caatcagttg ccagcctgaa agatggacat tcatttcgcg gcgggcggcg
     9721 caggaagtgt gaacggcgac tttgagcggt tcgcccttgt cttcgggttt gaaaccgatg
     9781 gcaagcagga aatgggtaaa ctcgatgatg cgcttggcgc aatccacagc cctttcctca
     9841 tactcactgc ctttaaacag cgtcggccaa tggtgcttca tcatgccgcc gcatgagccg
     9901 gacggtacga caatcggcca gttctcgggg aaaaggtcga gttgcgcttt ggcgacatcg
     9961 aaggcttcgg tcggatggcc ggatgaatag gcaggctggc cgcagcagct ttgcgccatc
    10021 gggaaatgga cgcgtatgcc ctgctgctcg attagggtaa tggcatccat gcctgcttcg
    10081 ggcataaaaa gatcaaggac gcaagtgccg aaaaaataga catcggtcgg attgctgtcg
    10141 tatcggatga tttttggggg aatggttgcg ctcatgtttt ttgataacgg gaaacccgtt
    10201 tttctctgta gaaaggtaag cgtttacttt aagtaattga ctgttgcggg tcaagtctaa
    10261 ttttaaaaaa taatccggtt tttcttacaa actgccccat aacgcttact gtaccttaat
    10321 ctgatggttt tcgataataa ttatcattac aatgcaatgt cggttcgttt gcttgtgaac
    10381 attcaagatg ccgactctga cggcattcag acagcatctg aaaacaataa cggcacaagg
    10441 atggggtaca atcgccctat ggaaaaaaac accctatccg cccgcgcacc atccccttgg
    10501 ctgccgcttc tgctggcaat tgccattttt atgcagatgt tggatgcgac cattttaaat
    10561 accgcactgc ctgaaattgc cgccgacctg aatgagtcgc cattgaacat gcagctggcg
    10621 gttatttcct acacgctgac ggttgccctg ctgattcctt tgagcggtta tttggcggac
    10681 aggttcggaa cgaaaaaagt ctttttcggt tcgattgccg tttttatgct cggatcggca
    10741 ttgtgcgccg catcgggttc gctgtttgaa ttgacgcttt cccgtgtcgt tcagggcatc
    10801 ggcggttcga tgctggttcc gataccgcgt ctgaccatct tgcgtgtgta cgacaagtcc
    10861 aagctgctca acgccatcaa ttatgcggtt atgcccgcat taatcggacc ggttttaggg
    10921 cctttggcgg gcggttatct ggtcgaatac gcttcgtggc actggatttt cctgctcaac
    10981 ctgcccatcg gtcttttggg tttcatattg ggacgcaaca tcatgcccga tattaaaggc
    11041 agcaatatct ctttagactt caaaggttat ctgatttttt ctgccgccgc gtgcctcttg
    11101 ttactttcgg cagaaagcct gtcgcacgcg ctgcctccgt attttgcact gttgccgctg
    11161 tgcggcggac tgctgtttgc acgccgttat ttccgacata tgaaaaccgc gtccaaaccg
    11221 atttattccg ccgacctgtt tctgatacgc actttccgtc tgggactggc gggcaatctg
    11281 ttcagccgtc tcggcatcag ctcgattcct tttctgatgc ccctgatgtt tcaagtcgct
    11341 ttcggcttcg gcgcaagcct gtcgggttgg ctggtcgccc ccgtcgccct gtcttcgctg
    11401 ctggtcaaac cgctgattgc accgctcatg aaacgtttcg gctaccgcac ggtactgctt
    11461 tggaacacca agctgcttgc cgccttcatc atgctgctcg ccctgcctga tggaaactcg
    11521 ccgctgtgga tttggatttt cctctcgctg gcgatcggcg cgtgcaactc cctacagttt
    11581 tctgccatga acacactgac cctcgccgat ttgcgcccgc aacagaccgg cagcggcaac
    11641 agcctgatgg cggtcaacca acagcttgcc atcagcatgg gcattgttgt cggcgcatta
    11701 atccttaaaa actggacatt tctgataccg gcttcttcag gtctgcattc cgccttccgt
    11761 atgaccctgc tcagtatcgg cggcattacc cttgcctcgt cgttggtttt caagcgtctg
    11821 cacgtttcag acggtgcaaa cctgacacgg aacacaccgt cctaaagcgg tccgcacgca
    11881 aaacttttac ccgtttcaac gtttggatta tgattccgca cttccatgcg cgccaacccc
    11941 aaaacacagg caatgccgtc tgaaaccata tccctgatga aaacacgcag cctaatttcc
    12001 cttttatgcc tccttctctg ttcatgttct tcatggttgc ccccactgga agaacggacg
    12061 gaaagccgtc atttcaatac ttccaaaccc gtccgcctgg acaacatcct gcaaatccgg
    12121 cacacccctc ataccaacgg gctatccgat atctatctgc tgaacgaccc ccacgaagcc
    12181 tttgccgccc gcgccgccct tatcgaatct gccgaacaca gcctcgattt gcaatactac
    12241 atctggcgca acgacatttc cggccgactg ctgttcaacc tcgtgtacct tgccgcagaa
    12301 cgcggtgtgc gcgtacgcct gctgttggac gacaacaaca cgcgcggatt ggacgacctc
    12361 ctgctcgccc tcgacagcca tcccaatatc gaagtgcgcc tgttcaaccc cttcgtcttc
    12421 cgcaaatggc gcgcactcgg ctacctgact gacttccccc gcctcaaccg ccgcatgcac
    12481 aacaaatcct ttaccgccga caaccgcgcc accatactcg gcggacgcaa tatcggcgac
    12541 gaatacttca aagtcggtga ggacaccgtt ttcgccgacc tggatatcct cgctaccggc
    12601 agcgtcgtcg gcgaagtatc gcacgacttc gaccgctact gggcaagcca ttccgcccac
    12661 aacgccacgc gcatcatccg cagcggcaac atcggcaagg gtcttcaagc actcggatac
    12721 aacgacgaaa cgtccagaca cgcgctcctg cgctaccgcg aaaccgtcga acagtcgccc
    12781 ctctaccaaa aaatacagac aggacgcatc gactggcaga gcgtccaaac ccgcctgatc
    12841 agcgacgacc ctgcaaaagg actcgaccgc gaccgccgca aatcgccgat tgccgggcgg
    12901 ctgcaagacg cgctcaaaca gcccgaaaaa agcgtctatc tggtttcacc ctatttcgtc
    12961 cccacaaaat ccggcacaga cgcactggca aaactggtgc aggacggcat agacgttacc
    13021 gttctgacca actcgctaca ggcgaccgac gttgccgccg tccattccgg ctatgtcaaa
    13081 taccgaaaac cgctgctcaa agccggcatc aaactctacg agctgcaacc caaccatgcc
    13141 gtccctgcca caaaagacaa aggcctgacc ggcagctccg taaccagcct gcacgccaaa
    13201 accttcattg tggacggcaa acgcatcttc atcggttcgt tcaacctcga cccccgttcc
    13261 gcgcgtctca acaccgaaat gggcgttgtt atcgaaagcc ccaaaatcgc agaacagatg
    13321 gagcgcaccc ttgccgatac cacccccgaa tacgcctacc gcgttaccct cgacaggcac
    13381 aaccgcctgc aatggcacga tcccgccacc cgaaaaacct acccgaacga acccgaagcc
    13441 aaactttgga aacgcatcgc cgcaaaaatc ctatccctgc tgcccataga aagtttatta
    13501 tagaaatata gcggattaac aaaaaccagt acgacgttgc ctcgccttag ctcaaagaga
    13561 acgattctct aaggtgctga agcaccaagt gaatcggttc cgtactattc gtactgtctg
    13621 cggcttcgtc gccttgtcct gatttttgtt aatccactat accgtctgaa acaccttcag
    13681 acggatatcc gaacccgcaa aggaaaaacc atgtttcccc ccgacaaaac ccttttcctc
    13741 tgtctcagcg cactgctcct cgcctcatgc ggcacaacct ccggcaaaca ccgccaaccg
    13801 aaacccaaac agacagtccg gcaaatccaa gccgtccgca tcagccacat cgaccgcaca
    13861 caaggctcgc aggaactcat gctccacagc ctcggactca tcggcacgcc ctacaaatgg
    13921 ggcggcagca gcaccgcaac cggcttcgat tgcagcggca tgattcaatt cgtttacaaa
    13981 aacgccctca acgtcaagct gccgcgcacc gcccgcgaca tggcggcggc aagccgcaaa
    14041 atccccgaca gccgccttaa ggccggcgac ctcgtattct tcaacaccgg cggcgcacac
    14101 cgctactcac acgtcggact ctatatcggc aacggcgaat tcatccatgc ccccagcagc
    14161 ggcaaaacca tcaaaaccga aaaactctcc acaccgtttt acgccaaaaa ctacctcggc
    14221 gcacatactt tctttacaga atgagccgcc cgccgcgccg cgccggaatc gcggcaaaat
    14281 gccgccttat tccgctgttt cggtcagcga tgagaacacg tcgaaataag tcgggaaggt
    14341 tttgtgggtg catttcggat cgttgatgac gacgggtacg tccaacagcg aaaccagcga
    14401 gaaacacatt gccatgcggt ggtcgtcgta agtgtcgatg acggcgtcgg gtgtcggcgt
    14461 ttcgggcggg gtgatgtgaa ttgcttcggc ttcttcggcg acttttgcac cgagtttgcg
    14521 caactcgttc gccattgcgg caatgcggtc ggtctctttg acgcgccacg aaccgatgtt
    14581 gcgaagcgtg caggtttgcc ctgtagcaag cgcgacgacg gcgagggtca tggcggcatc
    14641 ggggatatgg tttgcatcca aatcaaaggc ttggacggca cgttctttcg ggcgcgagac
    14701 ttcgacgaag ttttcgcccc aaaccacgtc cgcaccgatt ttttccagct cgcaggcaaa
    14761 ggcgacatcg ccctgtatgc tgtttgcgcc gataccggta acgcggacgg gcgtggcggc
    14821 aatcaaaccg gctgcgagga agtaggacgc gctggaggca tcgccttcga cgtgcaagtg
    14881 ttcgggcgcg tgatagtgcg catcggcggg aattttgaag acgcggtagc cttcattgat
    14941 aacctgtacg ccgaattgcg ccatcagttt taaagtaatg tcgatatagg gcttggaaat
    15001 taactcgccg accatacgga tttcaaacgc ctgcccggtc agcggcaacg ccattaaaag
    15061 ggcggtcaga aactggctgg atacattgcc tttaatcgga atcacgcgct cgccgttgtc
    15121 ttggcgttcg ccgatatgaa gcggcggata gtgttccttg ccgagatatt cgacatcggc
    15181 gccggcaatc cgcagtgcat cggccaaatc gccgatgggg cgttcgtgca tacgcgcaac
    15241 gccgtgcagg cgataatcgc cgcccaaaac ggcaagcgcg gcggtcagcg ggcggaacgc
    15301 cgtgcccgcg ttgcccaaaa acaaatcggc agtgcggttg gggaagcgtc cgcctgtgcc
    15361 gtgcactttc agacggtctt cggcaagata ttcgatttca acgccgagtt tatcgagtgc
    15421 ttcgagcata cggtcggtat cgtcggattt gagcagggaa tggatttcgc aagcattgtc
    15481 ggacaaggcg gcaagcagca gggtgcggtt gctgatgctt ttggagccgg gcagggcgac
    15541 agtggaaggt ttgagcgagg cggcagggag gcggagggat tcggtcatgg caaaacgtaa
    15601 atataaagat aaaaacagcc tgcattatac tggtgcaaat gccgtctgaa aaatctcagg
    15661 tttggcattt tcggttttaa agtccgtgaa tatggttttt tgtgccgaaa attgattatt
    15721 tttaaatttt ttgtttctaa aatttttttg ccggcatatt ttcggctttt gttgcggaca
    15781 tagcgtgttt gcggacggca cggcacgatt gccaatttca tagaatttgg tagaatagcc
    15841 gctgttcaac gacagacaag ccgccgattt tccgggcggc ttgtgttttt attttaatat
    15901 ccggacgcac aaaacaagac ggtatgcggt atgtttgctg ccgcaaaccg gtgtgccgga
    15961 atatggagaa aaaagaccga tgcaaaaaat ccccctgacc gtacgcggtg cggaattgct
    16021 gaaacaggaa ttgcagcagc ttaaaagcgt ggcgcgcccc gaagtgatcg aagcgattgc
    16081 tgaagccaga tcgcacggcg atttgtccga aaacgccgaa tatgaagccg ccaaagaacg
    16141 ccaaggcttt atcgaaggcc gcatttccga gctggaacac aaactttccg ttgcccacat
    16201 catcaatccg accgaaatcc acgccgaagg caaaatcgtg ttcggtacga cggttacgct
    16261 ggaagatttg gaaacggaag aacacgttac ctaccaaatt gtcggcgaag acgaagcgga
    16321 tatcaaacag ggcaaaatct atgtcggctc accgattgcc cgcgccttaa tcggcaagga
    16381 agagggcgat acggcggaag tccaagcccc gggcggcgtg cgcgaatacg acattatcga
    16441 agtccgatat atttgattcg gcttgatttc gatacacccg acacacgcag gaaattatag
    16501 tggattaata aaaatcagga caaggcgacg aagccg
//
LOCUS       DAWKOG010000029        16456 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.029, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000029 DAWKOG010000000
VERSION     DAWKOG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16456
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.029"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            209..1273
                     /locus_tag="WJK66_000731"
     CDS             209..1273
                     /locus_tag="WJK66_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEZ7578476.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIIRD
                     DVPTGEGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            1414..1737
                     /locus_tag="WJK66_000732"
     CDS             1414..1737
                     /locus_tag="WJK66_000732"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEZ7578477.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLAEKA
                     ARTES"
     gene            1809..2132
                     /locus_tag="WJK66_000733"
     CDS             1809..2132
                     /locus_tag="WJK66_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEZ7578478.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2179..2382
                     /gene="tatA"
                     /locus_tag="WJK66_000734"
     CDS             2179..2382
                     /gene="tatA"
                     /locus_tag="WJK66_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEZ7578479.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2386..3072
                     /gene="tatB"
                     /locus_tag="WJK66_000735"
     CDS             2386..3072
                     /gene="tatB"
                     /locus_tag="WJK66_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HEZ7578480.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKETGTDMEGNLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPFPDAANTLLDGISDVMPSERSYASAETLGDSGQTGSTAEPAE
                     TDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRDLRPKSR
                     AKPKLRVRKS"
     gene            3086..3856
                     /gene="tatC"
                     /locus_tag="WJK66_000736"
     CDS             3086..3856
                     /gene="tatC"
                     /locus_tag="WJK66_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEZ7578481.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGMMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLASVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKISVVTTGQLKRARPYVIVGAFVIAAIITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIRPPATT"
     gene            3909..4514
                     /gene="maf"
                     /locus_tag="WJK66_000737"
     CDS             3909..4514
                     /gene="maf"
                     /locus_tag="WJK66_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEZ7578482.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYHVIQLPAGIDESVKAGETPFAY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSDGIILGKPRSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGNAENRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRTPPFIRA"
     gene            4530..4883
                     /locus_tag="WJK66_000738"
     CDS             4530..4883
                     /locus_tag="WJK66_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEZ7578483.1"
                     /translation="MNRQTAYLLTSFSLIALIILSLAWELWIAPLRPGGSWLALKTLP
                     LCLPLSGILKKKIYTYQYSSMLILIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            4873..6687
                     /locus_tag="WJK66_000739"
     CDS             4873..6687
                     /locus_tag="WJK66_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase C-terminal domain-containing
                     protein"
                     /protein_id="HEZ7578484.1"
                     /translation="MPAETPVSGARPAAKLPIYILPCFLWIGIVPFTFALRQQPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPPISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWACRSLVAHYGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWEDTPL
                     FQNIIVYSGQGVIGHIGQRNNLGHYLMWGILASAYLNGQRKIPAALGVICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     NIHDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKHPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNAFSPATDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKALKYRPYSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            6877..7554
                     /locus_tag="WJK66_000740"
     CDS             6877..7554
                     /locus_tag="WJK66_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ7578485.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7568..8974
                     /locus_tag="WJK66_000741"
     CDS             7568..8974
                     /locus_tag="WJK66_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEZ7578486.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIIVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            9092..11518
                     /locus_tag="WJK66_000742"
     CDS             9092..11518
                     /locus_tag="WJK66_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEZ7578487.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGNADLIIAVTLPDSYDTLFKTCRKKQLRHIILIQ
                     DWDSLPAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSSDWLNRFGHSLHTKTAVLHHNP
                     EEDQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHCLARHCNFLISFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLNAHAPKTLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTARALIRLSEQTAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALAAANPSLLAEAL
                     HLPPYRHTTHNAVQFQFGSHPLYGDILTACCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTVIPEYRQHILGITLNLNGGQYTSDFLLKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     HLKTLSLKNNTTVTITPLLPEDAEAKQQFVRSLSPEARYTRFMTHTNELPAATLARLC
                     NPDYHCEAAWTAKDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLHTDHKIT"
     gene            11563..11808
                     /gene="rpsP"
                     /locus_tag="WJK66_000743"
     CDS             11563..11808
                     /gene="rpsP"
                     /locus_tag="WJK66_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEZ7578488.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            11824..12333
                     /gene="rimM"
                     /locus_tag="WJK66_000744"
     CDS             11824..12333
                     /gene="rimM"
                     /locus_tag="WJK66_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEZ7578489.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTISVTLEAGKVVNGELQVKFEGINDRDLAFSLRGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKT
                     ITADWGLDY"
     gene            12333..13082
                     /gene="trmD"
                     /locus_tag="WJK66_000745"
     CDS             12333..13082
                     /gene="trmD"
                     /locus_tag="WJK66_000745"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEZ7578490.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKTQSSQAAKVIYLSPQGKPLTHQKAVEL
                     AELPHLILLCGRYEGIDERLLQSSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLETIRQEQREIQS"
     gene            13098..13463
                     /gene="rplS"
                     /locus_tag="WJK66_000746"
     CDS             13098..13463
                     /gene="rplS"
                     /locus_tag="WJK66_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEZ7578491.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13785..14510
                     /locus_tag="WJK66_000747"
     CDS             13785..14510
                     /locus_tag="WJK66_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEZ7578492.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLSRQDIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFK
                     GINTAQKQRVHEALERVGMRQFARRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPSTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPDWCAS"
     gene            14545..15420
                     /locus_tag="WJK66_000748"
     CDS             14545..15420
                     /locus_tag="WJK66_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEZ7578493.1"
                     /translation="MNLYDLLLAPFAEFDFMRYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSSLTLITLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            15497..16411
                     /locus_tag="WJK66_000749"
     CDS             15497..16411
                     /locus_tag="WJK66_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEZ7578494.1"
                     /translation="MKHLKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQSLVGANQDTHAYHMTSSDIKKIRSAKLVLLNGLGLEAADVQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEFDPHVWNDPVLMSAYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHIEFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNIKALINAMKQ"
BASE COUNT         4267 a         5284 c         3612 g         3293 t
ORIGIN      
        1 ccgtctgaag ccgattcggt tttcagacga catcattatt tgacctgacg caaacagaaa
       61 gccttccgcg tgcttaaaca catctcttgt agtaaaatat ctaaacagat tcaactggaa
      121 acggtcaggc ttcttcacgc cattcccatt tcaaccgccg cctgccagat tcagacggta
      181 ttcctacttg aaaacaagga gcgttacaat gaaagcagca cgtttttacg ataaaggcga
      241 catccgcatc gaagacatcc ccgaaccgac cgtcgccccc ggcactgtcg gcatcaatgt
      301 cgcctggtgc ggcatctgcg gtactgacct gcacgaattc atggaaggcc cgattttcat
      361 tccgccttgc ggtcatccgc acccgatttc cggcgagtcc gcacccgtaa cgatgggaca
      421 cgagttctcc ggcgtggtct atgccgtcgg cgaaggcgtg gacgacatca aagtcggcca
      481 acacgtcgtg gtcgaaccct acatcatccg cgatgacgta ccgaccggag aaggcagcaa
      541 ctaccacctc tccaaagata tgaactttat cggcttgggc ggctgcggcg gcggtctgtc
      601 cgaaaaaatc gccgtcaaac gtcgttgggt gcatccgatt tccgacaaaa tcccgttgga
      661 tcaagccgct ttgatcgaac cgctgtctgt cggacaccac gcctatgtac gcagcggcgc
      721 gaaagcaggc gacgtcgcat tggtcggcgg cgcaggtccg atcggtttgc tgttggctgc
      781 cgtgttgaaa gccaaaggca tcaaagtcat catcaccgag ttgagcaaag cacgcaaaga
      841 caaagcgcgc gaatccggcg ttgccgacta catcctcgac ccgtccgaag tcgatgttgt
      901 cgaagaggtg aaaaaactga ccaacggcga aggcgtggac gtggcattcg aatgtaccag
      961 cgtgaacaaa gtgttggata ctttggtcga agcctgcaaa cctgccgcca atttggttat
     1021 cgtatccatc tggagccacc ctgcaaccgt caacgtccac agcgtcgtga tgaaagagct
     1081 ggatgtgcgc ggcacgattg cctactgtaa cgaccacgcc gaaaccatca aactggtcga
     1141 agaaggcaaa atcaacctcg aacccttcat cacccagcgc atcaagctgg acgaactggt
     1201 ttccgaaggc ttcgagcgtc tgatccacaa caacgaatcc gccgttaaaa ttattgtgag
     1261 tccaaacctg taagcaaatt acatttgcaa cagcacaaat gccgtctgaa acgctcagac
     1321 ggcattttcc aacgaagcgc aacaggcgta ttgcccgaga gcagccgttt agcctatcat
     1381 tccgaacaaa cagaaacaaa cagaaggaaa atcatgggag attccgtact atccgccatc
     1441 caacaaacca tcacccagcg aaaatctgcc aatccgtccg aatcttacgt cgcacagctc
     1501 ttgcataagg gcgaagacaa aatcctaaaa aaagtgattg aagaggcggg cgaagtgttg
     1561 atggcatcca aagacaaaaa cccgtcccac ctggtttacg aagttgccga cttatggttt
     1621 cacaccatga ttcttctgac acaccacgac ctgaaggcgg aagacgtatt ggacgaactt
     1681 gcgcgccgcc aaggtttgtc gggcttggcc gaaaaagccg ctcgcacaga atcttgaatt
     1741 tatattaaaa tccgcacttt cccacattca atccgtctga ccgctgttca gacggcatcg
     1801 gagccgttat ggacaactgt attttctgca aaatcgccgc caaagacatt ccggcgcaaa
     1861 ccgtctatga agacggcgaa atggtttgtt tcaaagacat caaccccgct gctccggttc
     1921 atctgctgct gattcccaaa gtccatttcg attcgttggc acacgccgcg cccgaacatc
     1981 agcccctttt gggaaaaatg atgctgaaag ttcccgaaat cgccaaagcg gcaggactgg
     2041 cagacggctt caaaaccctg atcaacaccg gaaaaggcgg cggacaagag gtcttccacc
     2101 tgcatataca catcatgggc acacccgtat aaaccgttat ttcacaatca acccctaata
     2161 cttacttaag gatacatcat gggcagtttt tctctgacgc actggattat cgtactgatt
     2221 atcgtcgttt tgatattcgg caccaaaaaa ctgcgcaacg tcggcaaaga cctcggcggt
     2281 gcggttcatg acttcaaaca ggggctgaac gaaggtacag acggcaaaga agcccaaaaa
     2341 gacgatgtaa tcgaacacaa aaaagacgaa gacaaagcgt aatttatgtt tgatttcggt
     2401 ttgggcgagc tggtttttgt cggcattatc gccctgattg tcctcggccc cgaacgcctg
     2461 cccgaggccg cccgcaccgc cggacggctc atcggcaggc tgcaacgctt tgtcggcagc
     2521 gtcaaacagg aatttgacac gcaaatcgaa ctggaagaac taaggaaggc aaagcaggaa
     2581 tttgaagctg ccgctgctca ggttcgagac agcctcaaag aaaccggtac ggatatggag
     2641 ggtaatctgc acgacatttc cgacggtctg aagccttggg aaaaactgcc cgaacagcgc
     2701 acgcctgctg atttcggtgt cgatgaaaac ggcaatccct ttcccgatgc ggcaaacacc
     2761 ctattagacg gcatttccga cgttatgccg tccgaacgtt cctacgcttc cgccgaaacc
     2821 cttggggaca gcgggcaaac cggcagtaca gccgaacccg cggaaaccga ccaagaccgt
     2881 gcatggcggg aatacctgac tgcttctgcc gccgcacccg tcgtacagac cgtcgaagtc
     2941 agctatatcg ataccgctgt tgaaacccct gttccgcata ccacttcgct gcgtaaacag
     3001 gcaataagcc gcaaacgcga tttgcgtccc aaatcccgcg ccaaacctaa attgcgcgtc
     3061 cgtaaatcat aaagagagca atccggtgtc cgaaacacaa aacgaacaac ccgtccaacc
     3121 gcttgtagag catctcatcg agctgcgccg ccgcctgatg tggacggttg tcggcatctt
     3181 agtctgcttt ttcggcatga tgccgtttgc ccaacaactc tatactttta tcgccgaccc
     3241 gctgatggca aacctgccca aagacaccag catgattgcc accgatgtca tcgcaccatt
     3301 tttcgtgccg gtcaaagtta ccctgatggc ggcattttta atttcgctgc cgcatacgct
     3361 ctaccaaatc tgggcatttg tcgcgcccgc actctaccaa aacgaaaaac gcctgattac
     3421 gccgctcgtc ctctccagcg tcagcctgtt tttcatcggc atggcatttg cctacttttt
     3481 ggttttcccc gtcattttca aattccttgc cagcgttacc cctgtcggtg tcaatatggc
     3541 gacagacatc gacaaatacc tgtcttttat cttaggcatg tttgttgcgt tcggcacaac
     3601 gtttgaagtt cccatcgtcg ttatcctgtt aaccaaaatc agcgtggtaa caaccggaca
     3661 gctcaaacgc gcccgcccct atgtgattgt cggcgcgttt gtcattgccg ccatcatcac
     3721 gccgcccgat gtgatttcac aaaccctgct tgccattccg ctgattctct tatacgaagc
     3781 aggtatttgg ttcggacgct ttttcacgcc acgttcagaa caggatggcg acatacggcc
     3841 gcctgcaaca acctgacact atgccgtccg aacctccgcc tcataccgca acagattaag
     3901 gaataccttt gaataccctc tatttaggtt caaacagccc gcgccgaatg gaaatcctga
     3961 cacagttggg ctaccacgtc atccaactgc ctgccggcat cgacgaatcc gttaaagccg
     4021 gcgaaacacc tttcgcttac gttcaaagga tggcagaaga aaaaaaccga accgccctga
     4081 ccctcttttg cgaaaccaac ggcacaatgc ccgatttccc cctgattacc gccgacacct
     4141 gcgtcgtttc agacggcatc atattgggca aaccccgctc ccaagccgaa gcaatcgaat
     4201 ttttaaaccg attgtccggc aaacaacata ccgtcctgac tgccgtctgc attcattatc
     4261 gcggcaatgc ggaaaaccgc gtccaaacca accgcgtcgt tttcaagccc ctgagttcgg
     4321 aagaaatttc cgcctatgtg caaagcggcg aaccgatgga caaagccggc gcctacgccg
     4381 tacaaggcat aggcggcatc tttatccaat ctatcgaagg cagcttcagc ggcattatgg
     4441 ggctgcccgt ttatgaaacc gtttcgatgt tgcaggattt gggataccgt accccacctt
     4501 tcatccgagc ctaacccgta aagaccgccg tgaacagaca aaccgcttac ctccttacct
     4561 ctttcagcct gatcgcactg ataatcctgt cccttgcttg ggaactgtgg attgcaccgt
     4621 tacgcccggg cggttcgtgg cttgcgctca aaaccctgcc cctctgcctg ccgctttcag
     4681 gcatcctgaa gaaaaaaatc tacacctacc aatacagctc catgttgatt ctgatttatt
     4741 ttgccgaagc cgtcatgcgc ctgttcgacg cctatccggc agaacaaatt tgcgccgcgc
     4801 tttccgcagt attcagcatc atcttcttca tatcctgcct gtcgttcgtc aaacaataca
     4861 aggaaacaaa caatgcccgc tgaaacgccc gtatccggtg cgcgccccgc cgccaaactg
     4921 ccgatttaca tcctgccctg cttcctttgg ataggcatcg tcccctttac cttcgcgctc
     4981 aggcagcaac cgtcgcccga cttttaccac gatgctgcag ccgcagccgg cctgattgtc
     5041 ctgttgttcc tcacggcagg aaaaaagctg tttgatgtca aaatcccacc catcagcttc
     5101 cttctgtttg caatggcggc gttttggtat cttcaggcac gcctgatgaa cctgatttac
     5161 cccggtatga acgacatcgt ctcttggatt ttcatcttgc tcgccgtcag cgcgtgggcc
     5221 tgccggagct tggtcgcaca ctacggacaa gaacgcatcg ttaccctgtt tgcctggtca
     5281 ctgcttatcg gctccctgct tcaatcctgt atcgtcgtca tccagtttgc cggctgggaa
     5341 gacacccctc tgtttcaaaa catcatcgtt tacagcgggc aaggcgtaat cggacacatc
     5401 gggcagcgca acaacctcgg acactacctc atgtggggca tactcgcctc cgcctacctc
     5461 aacggacaac gaaaaatccc cgccgccctc ggcgtaatct gcctgattat gcagaccgcc
     5521 gttttaggtt tggtcaattc gcgcaccatc ttgacctaca tagccgccat cgccctcatc
     5581 cttcccttct ggtatttccg ttcggacaaa tccaacaggc ggacgatgct cggcatagcc
     5641 gcagccgtat tccttaccgc gctgttccaa ttttccatga acaccattct ggaaaccttt
     5701 accggcatcc gctacgaaac tgccgtcgaa cgcgttgcca acggcggttt cacagacttg
     5761 ccgcgccaaa tcgaatggaa taaagccctt gccgccttcc agtccgcccc gatattcggg
     5821 cacggctgga acagttttgc ccaacaaacc ttcctcatca atgccgaaca gcacaacata
     5881 cacgacaacc tcctcagcaa cttgttcacc cattcccaca acatcgtcct ccaactcctt
     5941 gcagagatgg gaatcagcgg cacgcttctg gttgccgcaa ccctgctgac gggcattgcc
     6001 gggctgctga aacaccccct gacccccgca tcgcttttcc tgatctgcac gcttgccgtc
     6061 agtatgtgcc acagtatgct cgaatatcct ttgtggtatg tctatttcct catccccttc
     6121 ggactgatgc tcttcctgtc ccccgcagag gcttcagacg gcatcgcctt caaaaaagcc
     6181 gccaatctcg gcatactgac cgcctccgcc gccatattcg caggattgct gcacttggac
     6241 tggacataca cccggctggt taacgccttt tcccccgcca ctgacgacag tgccaaaacc
     6301 ctcaaccgga aaatcaacga gttgcgctat atttccgcaa acagtccgat gctgtccttt
     6361 tatgccgact tctccctcgt aaacttcgcc ctgccggaat accccgaaac ccagacttgg
     6421 gcggaagaag caaccctcaa agcactaaaa taccgcccct actccgccac ctaccgcatc
     6481 gccctctacc tgatgcggca aggcaaagtt gcagaagcaa aacaatggat gcgggcgaca
     6541 cagtcctatt acccctacct gatgccccga tacgccgacg aaatccgcaa actgcccgta
     6601 tgggcgccgc tgttgcccga actgctcaaa gactgcaaag ccttcgccgc cgcgcccggc
     6661 catccggaag caaaaccctg caaatgaccc gcgccggcgg atacggatac cgcccgaaat
     6721 gtaaagctcc atgcaagaca ttgcaaaaaa cagcaaaccg gtagggaaat acgctatcaa
     6781 aaaacattgc agccgtgtta agataaaccg tcaaacaatc ttttcacgcc ccgcccgaaa
     6841 cagggtcggg gcataccctt acgaaaagga aacaccatga gccgcgtatt actcgtagat
     6901 gacgatgccc tgctaaccga actgctgacc gaatacctga gcgccgaagg tctgaacgtc
     6961 cgcagcgttc ccgacgggga agcaggcgta caggaaatcc tgagcgggca gtacgatgta
     7021 gtcgtattgg attccatgat gcccaaaatg aacggcttgg atgtcttgaa aaacgtacgc
     7081 gcccgaagca ccgtccccat catcatgctg accgccaaag gcgacgacat cgaccgaatc
     7141 atcggcttgg aaatgggcgc ggacgactat gtccccaaac cctgcacacc acgcgaactc
     7201 ttggcacgca tcaatgccat cctgcgccgc gcacaacaca gcggcgaaca gaacaacgca
     7261 cccaacagca tctccgtcag cgatgtcgtc ctgtatcccg ccaaacgcca ggcatccgtc
     7321 aaagacatgc cgctcgaact gaccagcacc gaattcaacc tgctcgaagt cctgatgcgc
     7381 catgccggac aggtagtcag caaagaaacc ctgtccgtcg aagcactcga ccgcaagctg
     7441 gcaaaattcg accgcagtat cgacgtacac atctccagca tccgccacaa gttgggcgat
     7501 gcctctctga ttcaaaccgt acgcggcttg ggctacctgt ttgtcaaaaa ctgaaataaa
     7561 cagataaatg aaactgttcc aacgcatctt cgccacattt tgcgcggtta tcgtctgtgc
     7621 aatctttgtg gcgagttttt ctttctggct ggtgcagaac acccttgccg aaaaccagtt
     7681 caaccaacgc cgcaccatcg aaaccacttt gatgggcagc atcatttccg cattccgggc
     7741 acgcggggac gcgggtgcgc gcgaaatcct gacggaatgg aaagacagcc ccgtctcatc
     7801 gggcgtgtac gttatacagg gcgacgagaa aaaagatatc ctgaaccggt atatcgacag
     7861 ctataccatc gaacgcgccc ggcttttcgc cgccggacac ccgcattcca acctcgtcca
     7921 tatcgaatac gaccgcttcg gcgaagaata cctgttcttc accaaagact gggacaaact
     7981 ccaagcccgc cgcctgccca gccccctgtt gatccccggc ctgccgctcg ccccgatttg
     8041 gcacgaactc atcatattgt ccttcatcat catcgtcgga ctgctgatgg catatatcct
     8101 cgccggcaac attgccaaac ccatcagaat cttaggcaac ggcatggaca gggtggcaaa
     8161 cggagaactt gaaacccgta tctcccaaca ggtcgacgac cgcgacgacg aattgtccca
     8221 tcttgccatc caattcgaca aaatggtgga aaaactcgaa aaactcgttg ccaaagaacg
     8281 ccacctgctc catcacgtct cccatgaaat gcgttctccc cttgcgcgca tgcaggcaat
     8341 tgtcggactg attcaggcgc agccccaaaa acaggagcaa tatctcaaac ggctggaagg
     8401 cgaactgacc cgcatggata cgctggccgg ggaactgtta accctgtccc gtctcgaaac
     8461 ttccaatatg gctttggaaa aagaaagcct gaaactcctg cccttcctgg gcaacctggt
     8521 agaagacaat caaagcattg cccagaaaaa cggacaaacg gttaccctgt ctgccgacgg
     8581 aaaaatcccc gaaaacacaa ccatccttgc caacgaaagc tacctgtacc gcgccttcga
     8641 caacgtcatc cgcaacgccg tcaactacag tcccgaaggc agcaccatcc tgatcaacat
     8701 cggacaagac cacaaacact ggataatcga cgttaccgac aacggccccg gcgtggacga
     8761 aatgcagctc ccgcacatct tcaccgcttt ctaccgtgca gactccagtg ccaacaaacc
     8821 cggaacagga ctggggcttg cattgaccca acatattatt gaacagcact gcggcaaaat
     8881 catcgccgaa aacatcaaac cgaacggtct gcggatgcgc tttatcctgc ccaagaaaaa
     8941 aaccggttcc aaaacagaaa aaagtgcgaa ctgaccataa taccgtctga accggcttca
     9001 gacggcattg cacaaacagt taccccaaaa caaacacaca gcgggtgcat ctccaaaaat
     9061 cacgcacata ccgtccgcaa aggaatatat catgtcggca caaaccgatc cgggctactt
     9121 tttcatgccc aaccacatta ttctgatagg cgcgagcgaa cgtccgtaca gcctgggcga
     9181 acgcgtactc agcaacctgc tgagtacgcc ctttcaagga aaaatcaccc ccgtaaaccc
     9241 gcgccaccac accatagctg ggcttcccgc ctacaccagc ctcaacaaaa tccccggcaa
     9301 tgcagacctg attattgccg ttaccctacc cgacagttac gacaccctct tcaaaacctg
     9361 ccgcaaaaag cagctccgac acatcatcct catacaggac tgggacagcc tgcctgccgc
     9421 agaactgcac accgccgaaa ctgccatccg caaacaccac ggcaacggac tcaacatcac
     9481 cgcctgcacc accgcaggca tccaactgcc ctcactcgga cttaacatca gtacccaaga
     9541 cggatacgcc gcaggccata ccgccatact gaccggcaat gccgccgtca gccgccaaat
     9601 cgacaacatc ctgaacaaac tccgtcaagg cacatcccgc cacatcagcc tgcatcccgg
     9661 catcagcccc atcacttcct cagactggct caaccgcttc ggacacagcc tgcacaccaa
     9721 aaccgccgtc ctacaccaca accctgaaga ggatcagcgc aaactgttca gcgcaatccg
     9781 ccaatttacc cgccatacgc cgctgattct ccacatcacc tgccgcacga cagaaaccga
     9841 ccgtgccgta ctgcactgcc tcgcccgaca ctgcaacttc ctcatcagtt tcaacgccga
     9901 cgaccttgaa gccgcactgc gcgcccaact gtccgacctt ccacccctgt cccgactcga
     9961 catcctgtcc gacacgcctg ccgaatggct gaacgcgcac gcgccaaaaa ccctcgccct
    10021 ccactttccc aaccttcccc accacatccg caacggacac ctgaccggca cacccacacc
    10081 ctcaatctgc cacgacatcg cctcacgtca gcttgcccgc cccgacaccc aagccgtcct
    10141 gaccatcctc agcccctccg gacacgagga ttacaaaaaa acagcacgcg cccttatccg
    10201 cctgtccgaa cagaccgcca aacccctgct cgtcagcagc cccttttcag acggcataac
    10261 ccatttcgac acccccactc aggcaatccg caccctttcc taccgcaaca ccgccgccgc
    10321 cctgaaacag gcacagctcg acattgcacc gccgcagcca tgccgtctga aaacaccgca
    10381 accccaaaac atcaaaaaag cccttgcagc ggcaaatccc tccctgctcg ccgaagccct
    10441 gcacctcccc ccctaccggc acactaccca taacgccgtc caattccaat tcggcagcca
    10501 ccccctctat ggcgacatcc tgaccgcatg ctgcaacgga caaaccactg ccgtactccc
    10561 gccgtttacc acgctcgaca gccgccacct tgcccgcttt gccgaactcg acggcacaca
    10621 aaccctcgac cagttcctgc acacactgac cgtcattccc gaataccgcc aacacattct
    10681 cggcatcacc ctcaacctca acggcggaca atacaccagc gatttcctct taaagacacc
    10741 cgaaacacac gacacgccca aacgcaagaa cacaggcaaa gccgcccaaa ccctcgaaca
    10801 tgccgccgca aaaatgcaga gtgccgccgc atacctgaaa cacaaaaacc cgacagccgc
    10861 cgaatttctc cgccacacaa gcgaagccgc cgcagaactg ctcggcagca aaaccgaaac
    10921 cggagcagcc gtacccaacg tacttgcccc ctatcccgca gcacacctca aaacactgtc
    10981 cctaaaaaac aacacgaccg ttaccattac cccccttttg cccgaagacg cagaagccaa
    11041 acagcagttc gtccgcagcc tcagccccga agcacggtac acacgcttca tgacccacac
    11101 caacgaactg cccgcagcca cgttggcacg cctgtgcaac cccgattacc actgtgaagc
    11161 cgcatggacg gcaaaggatg ccgacagcaa catcgtcgcc gtcgtccgcc acagccgcct
    11221 gaaccgcaac gaatgcgaat tcggcatcac actggcggaa catatgcgcg gcagcggact
    11281 ggcacagaaa atgatggaac tcatcatcca aaccgccgca cagcaaggct accggactat
    11341 gagtgccgac attctcaaaa ccaatacccc catgatcaaa cttgctgaaa aatcaggatt
    11401 taccctcaag gaatcggaca ccgaaaaaaa cctgtaccgc gcatatctga accttgcggc
    11461 agacaaaaca acagaaaaaa caaataaaaa cttgcacacc gaccacaaaa taacctaaaa
    11521 tcggcagttt ccatatatcc gattttctca aaaggactca aaatggtagt tatccgtttg
    11581 gcacgcggcg gctcgaaaca ccgccccttc tacaacgtca tcgttaccga ttcgcgcagc
    11641 cgccgcgacg gccgcttcat cgaacgcgta ggcttctaca accccgtagc caacgaaaaa
    11701 caagagcgcg tccgcctcaa tgcagaccgc ctgaaccact ggattgcaca aggcgcgcaa
    11761 gtcagcgact ccgttgcaaa actgattaaa gaacaaaaag ccgcctaatt cacatttgcc
    11821 gccatgacag acactcaaaa ccgggtagcc atgggctaca tcaaaggcgt attcggcata
    11881 aaaggctggt tgaaaattgc cgccaacacc gaatattccg acagcctttt ggactacccc
    11941 gagtggcatt tggtcaagga cggcaaaacc atcagcgtta cccttgaagc cggaaaagtc
    12001 gtcaacggcg aactccaagt caaattcgaa ggcataaacg accgcgactt ggcattctca
    12061 ttgcgcggtt acaccatcga aataccccgt gaagcattcg ccccgacaga agaagacgaa
    12121 tactactgga cagacttggt cggcatgacc gtcgtcaaca aagaccatac cgttttaggc
    12181 aaggtaagca acctgatgga aaccggcgca aacgacgtat tgatgattga cggagaacac
    12241 gggcagattc tgattccgtt cgtttcccaa tatatcgaaa ccgtcgatac cggcagcaag
    12301 accattactg ccgactgggg tttggactac tgatgcttat ccaggcagtt accattttcc
    12361 ccgaaatgtt cgacagcatt acccgctacg gcgtaacggg acgcgcgaac agacagggaa
    12421 tctggcagtt tgaagcagtc aatccccgaa agtttgccga caacagattg ggctacatcg
    12481 acgaccgccc gttcggcggc ggcccgggaa tgattatgat ggctccgccg cttcatgcgg
    12541 caatagaaca cgccaaaaca caatcctccc aagctgcaaa agtcatctac ctcagccccc
    12601 aagggaaacc gctgacacac caaaaagcgg tagaactggc agaacttccg catctgattc
    12661 tgctgtgcgg acggtatgag ggcatagacg aaaggcttct gcaaagcagc gtcgatgaag
    12721 aaatcagcat cggagacttc gttgtttccg gcggagagct tcccgccatg atgctgatgg
    12781 atgcggtatt gaggctcgta cccggcgtat tgggcgatat gcagtctgcc gaacaggatt
    12841 cgttctcaag cggcattttg gattgccccc actacaccaa acccttagaa tttcaaggca
    12901 tggctgttcc ggaagtattg cgttccggca atcatggctt gatagcggaa tggcggttgg
    12961 aacaatcgct gcgccgcacc ttggagcgca gacccgatct tttggaaaag cgcgttttaa
    13021 tcccaaagga atcccgcctc ttagaaacca tccggcaaga gcaacgggaa atccaatcat
    13081 aatttaggaa aaaaacaatg aacctgattc aacagctcga gcaagaagaa attgcccgcc
    13141 tgaacaaaga aatccccgaa ttcgcaccgg gcgacaccgt agtcgtatcc gtacgcgtcg
    13201 tggaaggtac ccgcagccgt ctgcaagcct acgaaggcgt tgtcattgca cgccgcaacc
    13261 gtggtctgaa cagcaacttc atcgtccgta aaatttccag cggcgaaggt gtggaacgta
    13321 ctttccaact gtactcccct accgttgaga aaatcgaagt caaacgccgt ggcgatgtcc
    13381 gccgtgccaa actttactat ctgcgcggcc tgaccggcaa agctgcacgc atcaaagaaa
    13441 aactgcctgc acgcaaaggt tgattcaaac ccgttttccc caatgccgtc tgaaccttct
    13501 gacggcattt cttattgccg caacatccgc aacggaacag caccaagaag cacacccctt
    13561 ccggcacatc ccgaatcccc gtccgaaccg aacaaaattt acagaccaaa cgttatacag
    13621 tatcatatca gccatataat aataaccgct ttaccgcttt ccctttcaaa accgcaaatc
    13681 atcatgacac cttccctttt actatcagga ttgaccttcc gcctcgtcct tgccctgatt
    13741 gccgtatccc ttttatgggg cgtttacttt tgggcagtat ccgcatgagc atcattgtcg
    13801 aaaacctgac cgtcagctac cgccgccgtc ctgccgtaca ccacgttgac attacttttg
    13861 aagaacatag tatgtgggcg gttttcggtc ccaacggcgc agggaaatcc acctttctca
    13921 aatccttgat gggattgcag cctatcgata caggcagcat ccggctggat ggattgagcc
    13981 gccaagacat cgcctacctt ccccagcagt ccgatatcga ccgctcccaa cctatgactg
    14041 ttttcgactt ggcggcaatg gggctttggt atgaaatcgg ctttttcaaa gggataaata
    14101 ccgctcaaaa acaacgcgtt cacgaagcct tggagcgtgt aggaatgcgg cagtttgcac
    14161 gccgccaaat cgcccacctc tcaaacggac aattccaacg cgtccttttt gcccgaatgc
    14221 tggttcaaaa tgcaaaattc ctgcttttgg acgaaccttt caacgccgtt gatgcacgga
    14281 cgacctacga acttctcgac gtattgcaga aatgccattg cggcggacac gctatcattg
    14341 ccgtactgca cgattacgaa caagtccgtg cctactttcc cagtaccctg ctgctcgccc
    14401 gagaaaaaat tgcggcaggc gcaaccgaga ccattctgac agaaccctac ctcgcccaag
    14461 ccaacgccaa aatgcagcaa caggaaagcc ccgactggtg cgcctcataa atactgcccg
    14521 aaaccacgcc ccacaccaga caccatgaat ctctacgacc tgctccttgc cccctttgca
    14581 gaattcgact ttatgcgcta cgccctcgca tccgtcttct gcctgtccct cagtgccgca
    14641 cccgtcggcg tattcctcgt catgcgccgt atgagcctga taggcgacgc attgagccac
    14701 gccgtcctgc ccggtgccgc cgtcggctac atgtttgccg gcttgagcct gcccgccatg
    14761 ggtttgggcg gcgtagccgc aggcatgctg atggcactgc ttgccggact cgtcagccgc
    14821 ttcaccaccc tgaaagaaga tgccaacttt gctgcctttt atctcagcag cctcgccatc
    14881 ggcgtagtcc tcgtcagcaa aaacgggagc agcgtcgatt tgctccacct ccttttcggc
    14941 tctgtacttg ccgtcgatat tcctgccctg cagctcatcg ccgccgtctc cagcctcacg
    15001 ctcattaccc ttgccgtcat ctaccgcccg ctcgtactcg aaagcatcga ccccctgttt
    15061 ctcaaatccg tcggcggcaa aggcgggctt tggcacgtcc tctttctcgt cctggtcgtc
    15121 atgaacctcg tatccggctt tcaagccctc ggcacactca tgtccgtcgg actcatgatg
    15181 ctgccagcca ttaccgcccg cctgtgggcg aagcacatgg gcgcactcat cctcctatcc
    15241 gttctgacag ccctgctgtg cggcttgagc ggactgctca tttcctacca catcgaaatt
    15301 ccctccggtc ccgccatcat cctctgttgc agcgtccttt atctcttttc cgtcatactc
    15361 ggcaaagaag gcggcattct gaccaaatgg ctcaaaaacc accgccacca caccacctga
    15421 ccccgcaagc cgcttgaaca aacaggcagc ttgttttacc ataacagatg ttacgttata
    15481 tcacaggaga acagttatga aacacctcaa actcaccctt attgccgcat tgctaaccac
    15541 cgccgcaact gccgcccccc tgcccgtcgt aaccagcttc agcattttag gcgacgttgc
    15601 caagcaaatc ggcggagagc gcgtatccat acaaagtttg gtcggagcca accaagatac
    15661 tcatgcctat cacatgacca gtagcgacat taaaaaaatc cgcagtgcaa aactcgtcct
    15721 gctcaacggc ttaggacttg aagctgccga tgtacaacgt gccgtcaaac agagcaaagt
    15781 atcctatgcc gaagcgacca aaggcatcca acccctcaaa gccgaagaag aaggcggaca
    15841 ccatcatgac cacgaccatg accacgaagg acaccaccat gaccacggcg aatttgaccc
    15901 gcacgtctgg aacgaccccg tccttatgtc cgcctatgcc caaaacgtcg ccgaagccct
    15961 gataaaggcc gaccccgaag gcaaagttta ttatcaacaa cgcttgggca actaccaaat
    16021 gcagcttaaa aaactgcaca gcgacgcaca agccgcattt aacgccgtcc ctgccgccaa
    16081 acgcaaagtc ctgaccgggc acgatgcctt ttcctacatg ggcaaacgtt accatattga
    16141 attcatcgcc ccgcaaggtg tgagcagcga agccgagcct tcagccaaac aagtcgccgc
    16201 catcatccga caaatcaaac gcgaaggcat caaagccgtc tttaccgaaa acatcaagga
    16261 cacccgtatg gttgaccgta tcgccaaaga aaccggtgtc aacgtcagcg gcaaactgta
    16321 ttccgacgca ctcggcaacg cgcccgcaga cacctacatc ggcatgtacc gccacaacat
    16381 caaagccttg atcaacgcga tgaagcaata aaaccgtttg attggtttga atttgaaaag
    16441 gcaaaaggcc gtctga
//
LOCUS       DAWKOG010000030        16138 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.030, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000030 DAWKOG010000000
VERSION     DAWKOG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16138
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.030"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            6..81
                     /locus_tag="WJK66_000750"
     tRNA            6..81
                     /locus_tag="WJK66_000750"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Trp,seq:cca)
     gene            196..474
                     /gene="secE"
                     /locus_tag="WJK66_000751"
     CDS             196..474
                     /gene="secE"
                     /locus_tag="WJK66_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEZ7578495.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWFEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            476..1012
                     /gene="nusG"
                     /locus_tag="WJK66_000752"
     CDS             476..1012
                     /gene="nusG"
                     /locus_tag="WJK66_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEZ7578496.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            1113..1547
                     /gene="rplK"
                     /locus_tag="WJK66_000753"
     CDS             1113..1547
                     /gene="rplK"
                     /locus_tag="WJK66_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEZ7578497.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKEPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            1547..2242
                     /gene="rplA"
                     /locus_tag="WJK66_000754"
     CDS             1547..2242
                     /gene="rplA"
                     /locus_tag="WJK66_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEZ7578498.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKITRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            2472..2972
                     /gene="rplJ"
                     /locus_tag="WJK66_000755"
     CDS             2472..2972
                     /gene="rplJ"
                     /locus_tag="WJK66_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEZ7578499.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            3030..3401
                     /gene="rplL"
                     /locus_tag="WJK66_000756"
     CDS             3030..3401
                     /gene="rplL"
                     /locus_tag="WJK66_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEZ7578500.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            3592..7770
                     /gene="rpoB"
                     /locus_tag="WJK66_000757"
     CDS             3592..7770
                     /gene="rpoB"
                     /locus_tag="WJK66_000757"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEZ7578501.1"
                     /translation="MSYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVVRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNRLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            7922..12097
                     /gene="rpoC"
                     /locus_tag="WJK66_000758"
     CDS             7922..12097
                     /gene="rpoC"
                     /locus_tag="WJK66_000758"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEZ7578502.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRDGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIVTDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQG
                     VEQETAETQVTDE"
     gene            12388..12645
                     /locus_tag="WJK66_000759"
     CDS             12388..12645
                     /locus_tag="WJK66_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578503.1"
                     /translation="MKPRNLFFAGCLLTSATFAEDIGVPVELINVGNRIAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGDRMGMPINIG"
     gene            12642..12791
                     /locus_tag="WJK66_000760"
     CDS             12642..12791
                     /locus_tag="WJK66_000760"
                     /inference="COORDINATES: protein motif:HMM:NF016462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ7578504.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKINSNMPAFYSRSSKEWFVS
                     IEKTY"
     gene            12970..13341
                     /gene="rpsL"
                     /locus_tag="WJK66_000761"
     CDS             12970..13341
                     /gene="rpsL"
                     /locus_tag="WJK66_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEZ7578505.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            13459..13929
                     /gene="rpsG"
                     /locus_tag="WJK66_000762"
     CDS             13459..13929
                     /gene="rpsG"
                     /locus_tag="WJK66_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEZ7578506.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            13948..16053
                     /gene="fusA"
                     /locus_tag="WJK66_000763"
     CDS             13948..16053
                     /gene="fusA"
                     /locus_tag="WJK66_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEZ7578507.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKI
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
BASE COUNT         4289 a         3585 c         4182 g         4082 t
ORIGIN      
        1 tttagaggcc aatagctcaa ttggtagagt atcggtctcc aaaaccgagg gttgggggtt
       61 cgagaccctc ttggcctgcc aaataaaaaa ttaaccggcc ttgtgtcggt taattttttt
      121 gtatttgtta tttagtaaac tctcttgcca tttacatgga ttgagaatag acagatgcta
      181 tgatggataa ataatatgac agaacatacg cctgaaaaaa agaacgttaa agtggatcaa
      241 ctggttgttc aagataaaga atctgcatct aattccggta aggaagggtt ttttgcatat
      301 ttctcaaatt cttggttcga attcaaaaag gtggtttggc ctaagcgtga agatgctgtc
      361 agaatgactg tatttgttat agtgtttgtt gctgtgcttt ctatatttat ctatgcggca
      421 gatacagcaa tttcgtggtt attttttgat gtattgctga gaagggaagg ttgagatgtc
      481 gaaaaaatgg tatgttgtac aggcgtattc ggggtttgag aagaatgtcc aacgaatatt
      541 ggaagagcgc attgcccgtg aggagatggg agattatttc ggacaaattc tggtgcctgt
      601 agagaaagtt gttgatatcc gcaatggtcg taagactatt agtgaaagaa agtcatatcc
      661 tggttatgtg ctagttgaga tggaaatgac agatgactct tggcatcttg taaaaagcac
      721 cccccgtgtt tccggtttta ttggagggag ggctaataga cctacgccga ttagtcagag
      781 agaggctgaa attattttac agcaggttca gaccggcata gagaagccga aaccaaaagt
      841 tgaatttgag gtcggtcaac aggttcgtgt aaatgaaggg ccgtttgcgg attttaacgg
      901 ggtggttgag gaggtcaatt atgaacggaa taagttacgc gtgtctgttc agatatttgg
      961 tagagaaaca cccgttgagc tggagttcag ccaggttgaa aagattaact gatttttata
     1021 cttgaaaaaa aagcaataag aggatagaat caaaaattaa cttggggagc ggaaatggtt
     1081 ccgcgtctta cccgttttta ggagttcgtt aagtggcaaa gaaaattatc ggctatatta
     1141 aactgcaaat tcctgcaggt aaagccaatc catctcctcc ggttggtcct gctttgggtc
     1201 agcgcggttt gaatattatg gaattttgta aggcatttaa tgctgcaacc caaggtatgg
     1261 agcctggctt accgattccg gttgtgatta ctgcatttgc agataaatca ttcacatttg
     1321 tgatgaaaac cccgccagct tctatcttgt tgaaaaaggc tgccggtttg caaaaaggta
     1381 gttctaatcc tctgaccaac aaagtgggta aattgacccg tgcccagttg gaagaaattg
     1441 ctaaaactaa agagcctgat ttgactgctg ctgacttgga tgcggctgtc cgtactatag
     1501 caggttctgc tcgctcaatg ggcttggatg tggagggtgt tgtataatgg ctaaagtatc
     1561 taaacgcttg aaagctcttc gctcttctgt ggaagccaat aaattatatg caattgatga
     1621 agcaattgct ttggtaaaaa aagcagcgac tgctaaattt gacgagtctg ttgacgtatc
     1681 tttcaacttg ggcgttgatc cgcgtaaatc tgaccaagtt atccgtggtt cagtcgttct
     1741 gcctaaaggc accggtaaga taactcgcgt agctgtattt actcaaggtg caaatgcaga
     1801 agctgctaaa gaagctggtg cagatatcgt cggtttcgaa gatttggctg ctgaaatcaa
     1861 agcaggcaat ctgaactttg atgtcgttat tgcttctccc gatgcaatgc gtattgttgg
     1921 tcagttgggt actattttgg gtcctcgagg cttgatgcca aaccctaaag taggtactgt
     1981 tactcctaac gttgctgaag cagtaaaaaa tgcaaaagca ggtcaagtac aataccgtac
     2041 agataaagca ggtatcgttc atgcaacgat tggtcgtgct tctttcgctg aagctgactt
     2101 gaaagagaac tttgatgcgt tgctggatgc tatcgttaaa gccaagcctg ctgccgctaa
     2161 aggtcagtat ctgaaaaaag ttgctgtgtc tagcaccatg ggtttgggta ttcgcgttga
     2221 tacatcaagc gtaaataact aatcttaagg aattttcaag cagtttggtt ttctgggctg
     2281 cttgaatttg ggctacttaa aattaagtag atgtccaaga ccgtagggat cgtaagattt
     2341 aatcgtaact gccctacgca gacggtagtc ctgaaacaca ttgcaagatt gcttgtaaga
     2401 tgtcttttta ggttaccgcg ctggtgggat atcgttttgg tatcctgttt ataaacagtg
     2461 ggaggtagac cttgagtctc aatattgaaa ccaagaaagt ggcggtcgag gaaattagcg
     2521 cggcaattgc taatgctcaa accctcgtag tcgctgaata tcgcggtatc agtgtttcca
     2581 gtatgactga gcttcgtgcg aatgcacgta aagaaggcgt ttatttgcgc gttctgaaaa
     2641 atactttggc tcgtcgtgca gtgcaaggta cttcatttgc agaattggcc gatcaaatgg
     2701 ttggtccgtt ggtttacgct gcttctgaag atgctgttgc tgctgctaaa gtgttgcacc
     2761 aattcgcgaa aaaagatgac aaaattgtcg ttaaagccgg ttcttacaat ggcgaagtaa
     2821 tgaatgctgc tcaggttgct gagttggctt ctattccgag ccgcgaagag ctgttgtcca
     2881 aactgttgtt cgttatgcaa gctcctgtat cgggctttgc gcgcggtttg gctgctttgg
     2941 cagagaaaaa agccggcgaa gaagccgctt aatcgatttt gtttctgtta atcaattatt
     3001 ttttaataca atatttggag taaaatagca tggctattac taaagaagac attttggaag
     3061 cagttggttc tttgaccgta atggaattga acgacttggt taaagctttt gaagaaaaat
     3121 tcggtgtttc tgctgctgct gttgcagttg caggtcctgc tggtgccggt gctgccgatg
     3181 ctgaagaaaa aaccgaattt gatgtcgttt tggcttctgc cggtgatcaa aaagtcggcg
     3241 tgattaaagt tgtccgtgca attaccggtt tgggtctgaa agaagctaaa gacatcgttg
     3301 acggtgcacc taaaaccatt aaagagggtg tttctaaagc tgaagccgaa gacatccaaa
     3361 aacaactgga agaagccggc gctaaagtcg aaatcaaata atttgatgct tcttatgaag
     3421 gctggcagtt ttctgccagc cttattttgc ttcttaaaat aagcattaag tattgtttac
     3481 atttatttgc atagttttta tcaaatcatt gcaaataaat gtaaacatca gattgatgcg
     3541 tgccgttgct ttcagacggc ctattattga aaattacttt tcggagtgta tatgagctat
     3601 tcgtttaccg agaaaaaacg tatccgtaag agttttgcaa agcgggaaaa tgttttggaa
     3661 gttcctttct tgctggcaac ccaaattgat tcttatgcga agtttttgca gctggaaaat
     3721 gcttttgaca aacgtaccga tgacggtctg caggcggcat ttaattctat tttcccgatt
     3781 gtgagccata acggttatgc gcgattggag tttgtgcatt acacattggg cgagcctttg
     3841 ttcgatattc ccgaatgtca gttgcgcgga atcacttatg cagccccctt gcgcgcgcgt
     3901 atccgtttgg tgattttgga taaggaagca tctaaaccga cggtaaaaga agttcgtgaa
     3961 aacgaagtgt atatgggcga aattccgttg atgaccccga gcggttcttt tgtgattaac
     4021 ggcacagagc gtgtgattgt ttcccagttg caccgttcgc ccggcgtgtt tttcgagcat
     4081 gacaaaggta agacgcactc ttccggcaaa ttgttattct ccgcccgcat cattccctac
     4141 cgtggttcat ggttggattt tgaatttgat ccgaaagatt tgctgtattt ccgtatcgac
     4201 cgccgccgta aaatgccggt aacgattttg ttgaaggctt tgggctacaa caatgagcaa
     4261 atcttggata ttttctacga caaagaaacg ttctatttgt cttcaaacgg tgttcaaacc
     4321 gatttggtcg caggccgtct gaaaggcgaa actgccaagg tcgatatctt ggataaagaa
     4381 ggcaatgtat tggttgccaa aggtaagcgc attactgcga aaaatatccg tgatattacc
     4441 aatgcaggcc tgacccgttt ggatgtagaa ccagaaagcc tgctgggcaa agcattggct
     4501 gccgacctga ttgattcgga aaccggcgag gtattggctt ctgccaatga tgaaattaca
     4561 gaagagttgt tggccaaatt tgatatcaac ggcgtaaaag aaattacgac cctttacatc
     4621 aatgagttgg atcagggtgc ttatatctcc aataccttgc gtacggatga gactgccggc
     4681 cggcaggcgg cgcgtgttgc gatttaccgt atgatgcgtc cgggcgaacc gcccactgaa
     4741 gaggcggtcg agcaattgtt taaccgcttg ttcttcagtg aagacagcta cgatttgtcc
     4801 cgcgtaggcc gtatgaaatt taatacgcgc acatacgaac aaaaactgtc cgaagcccaa
     4861 caaaactctt ggtacggccg cctgctgaac gaaacgtttg ccggtgctgc cgacaaaggc
     4921 ggttatgtcc tgagcgtcga agatattgtc gcctcgattg cgactttggt cgagttgcgt
     4981 aacggccatg gcgaagtgga cgatatcgat cacttgggca accgccgagt acgttcggta
     5041 ggcgagctga ctgaaaacca attccgtagc ggtttggccc gtgtggaacg tgccgtaaaa
     5101 gaacgtttga atcaggcgga atcagaaaac ttgatgccgc acgatttgat taatgcaaaa
     5161 cctgtttctg ctgctattaa agaattcttc ggctccagcc aattgagtca gtttatggat
     5221 cagaccaatc ccttgtctga agtaacccat aaacgccgcg tatccgcatt gggtccgggc
     5281 ggtttgaccc gcgaacgtgc aggatttgag gtgcgggacg tgcatccgac ccactacggc
     5341 cgcgtatgtc cgattgaaac gcctgaaggt ccgaacatcg gtttgatcaa ctcactgtcc
     5401 gtttatgcgc gcaccaatga ttacggtttc ttggaaacgc cttaccgccg cgttatcgac
     5461 ggcaaagtaa ccgaggaaat cgattacttg tctgccatcg aagaaggccg ctatgtgatt
     5521 gcacaggcga atgccgattt ggattcagat ggcaatctga ttggcgattt ggttacctgt
     5581 cgtgaaaaag gcgaaaccat tatggcaacg cccgaccgcg tccaatatat ggacgtggca
     5641 accggccagg tggtatccgt tgcggcatcc ctgattccgt tcttggaaca tgatgacgcg
     5701 aaccgcgcat tgatgggtgc caacatgcaa cgtcaggcag tgccttgctt gcgtcctgaa
     5761 aaaccgatgg tcggtaccgg tatcgagcgt tccgttgccg ttgactctgc tactgcaatc
     5821 gttgcccgcc gaggcggcgt ggtcgagtat gtcgatgcca accgcgtcgt cgttcgtgtt
     5881 catgacgatg aagcgactgc cggcgaagtg ggtgtcgata tttacaactt ggttaaattt
     5941 acccgttcca atcaatctac taacatcaac caacgtccgg ctgtcaaagc aggcgatgtt
     6001 ttgcaacgcg gcgatttggt ggccgatggc gcgtccaccg atttgggcga attggctttg
     6061 ggtcaaaata tgaccatcgc cttcatgccg tggaacggtt acaactacga agactcgatt
     6121 ctgatttccg aaaaagtggc tgcggacgac cgctatactt cgattcacat tgaggaattg
     6181 aatgtcgttg cccgcgatac caagctgggt gcggaagaca ttacccgcga tattccgaac
     6241 ttgtccgagc gtatgcaaaa ccgtttggac gaatccggta tcgtttacat cggtgcggaa
     6301 gtagaagccg gcgatgtgtt ggtaggcaag gtaacgccta aaggcgaaac ccagctgacg
     6361 ccggaagaaa aactgctgcg cgccatcttc ggtgaaaaag catctgacgt aaaagatact
     6421 tcattgcgta tgcctaccgg catgagcggt accgttatcg acgttcaagt cttcactcgt
     6481 gaaggtattc aacgcgacaa acgtgctcaa tccattatcg attccgagtt gaaacgctac
     6541 cgtttggatt tgaacgacca attgcgtatt ttcgacaacg acgcattcga ccgtatcgag
     6601 cgtatgattg tcggtcagaa agccaacggc ggcccgatga agctggccaa aggcagtgaa
     6661 atcacgaccg aatatctggc gggtctgccg agcaggcacg attggttcga tatccgtctg
     6721 accgatgaag atttggccaa gcagttggaa ctgattaaag tgagcctgca acaaaaacgc
     6781 gaagaagcgg acgagttata cgaaatcaag aagaaaaaac tgacccaagg cgacgaattg
     6841 caacccggcg tacaaaaaat ggtgaaagtt tttatcgcca tcaaacgccg tctgcaagcc
     6901 ggcgacaaaa tggcgggccg ccacggtaac aaaggcgtgg tatcgcgcat tctgccagtg
     6961 gaagacatgc cttacatggc ggacggccgt ccggtagaca tcgtactgaa cccattgggc
     7021 gtaccttccc gtatgaacat cggtcagatt ttggaagttc acttgggttg ggcagcaaaa
     7081 ggtatcggcg agcgtatcga ccgtatgctg aaagagcaac gcaaagcagg cgagttgcgc
     7141 gagttcttga acagactcta caacggcagc ggtaagaaag aagatttgga tgccctgact
     7201 gatgaagaaa tcatcgaact ggcctccaac ctgcgcaaag gtgcatcttt cgcctctcct
     7261 gtattcgacg gtgcgaaaga gtctgaaatc cgcgaaatgc tgaacttggc ttatccgagc
     7321 gacgatcctg aggttgaaaa actgggcttc aacgacagta aaacccaaat cacgctgtat
     7381 gacggccgtt caggcgaagc atttgaccgc aaggttacag taggtgtgat gcactatctg
     7441 aaactgcacc acttggttga cgaaaaaatg cacgcccgtt ctaccggtcc gtacagtctg
     7501 gttacccagc agcctttggg cggtaaagcc cagttcggcg gccaacgttt cggcgagatg
     7561 gaggtttggg cattggaagc atacggcgcg gcatacacgc tgcaagagat gttgactgtg
     7621 aagtctgacg acgtgaacgg tcgtaccaaa atgtacgaaa acatcgtcaa aggcgaacac
     7681 aaaatcgatg ccggtatgcc cgagtccttc aacgtattgg tcaaagagat tcgctcactg
     7741 ggcttggata tcgatttgga acgctactga tacgggtttc agacggcata aggggagctg
     7801 ttctgcaggt atgcggggca gccgacaatg tttaaaaacg aaatgccgtc tgaaaacact
     7861 gtacctctat ccatatcgaa aatccgccat gcggtaaaaa tacttccttc aaggagcaaa
     7921 aatgaatttg ttgaacttat ttaatccgtt gcaaactgcc ggcatggaag aagagtttga
     7981 tgccatcaaa atcggtattg cctctcccga aaccatccgc tcatggtctt atggcgaagt
     8041 caaaaaacct gaaaccatca actaccgtac gttcaaacct gagcgtgacg gtttgttctg
     8101 tgccaaaatc tttggcccgg tcaaagacta cgaatgcttg tgcggaaaat acaaacgctt
     8161 gaaatttaaa ggtgtaactt gtgaaaaatg cggcgtggaa gtaaccctgt ccaaagtgcg
     8221 ccgcgaacgc atgggccata tcgaattggc tgcgcccgtc gcacatattt ggttcttaaa
     8281 atccctgcct tcccgcttgg gtatggtgtt agacatgact ttgcgcgata tcgaacgcgt
     8341 attgtacttt gaagcatttg tggtaaccga tcccggtatg actccgctgc aacgccgcca
     8401 attgctgact gaagacgatt actacaacaa gctggacgaa tacggcgacg atttcgatgc
     8461 caaaatgggt gcggaaggta tccgcgaatt gctgcgtacc ctgaatgtag cgggcgaaat
     8521 cgaaatcctg cgccaagagt tggaatcgac cggttccgac accaaaatca aaaaaatcgc
     8581 caaacgcttg aaagtattgg aagctttcca tcgttccggt atgaaactgg aatggatgat
     8641 tatggatgtg ctgccggtat tgccgcctga tttgcgtccg ttggttccat tggatggtgg
     8701 tcgttttgcc acttccgatt tgaacgattt gtaccgccgc gttattaacc gtaacaaccg
     8761 tctgaaacgt ctgttggaac tgcatgctcc tgacatcatc gtccgcaacg aaaaacgtat
     8821 gttgcaagaa gcagttgact cgctgttgga taacggccgt cgcggtaaag ccatgaccgg
     8881 cgccaacaaa cgcccgctga aatcattggc agacatgatt aaaggtaagg gtggccgctt
     8941 ccgtcaaaac ctgctgggca aacgtgtgga ctactccggc cgttccgtga ttaccgtagg
     9001 cccgtacctg cgtctgcacc aatgcggttt gccgaaaaaa atggctttgg aactgttcaa
     9061 accgttcatt ttccacaaat tggaaaaaca aggtttggcc tctaccgtta aagcagcgaa
     9121 aaaattggta gagcaagaag taccggaagt atgggacatc ttggaagaag tcatccgcga
     9181 acatccgatt atgctgaacc gtgcgccgac cctgcaccgt ttgggtattc aagcgttcga
     9241 acctatcttg attgaaggta aagcgattca gttgcaccca ttggtgtgtg ctgcgttcaa
     9301 cgccgacttt gacggcgacc aaatggcggt acacgttcca ttgagcttgg aagcacaaat
     9361 ggaagcacgc acgctgatgc tggcttcaaa caacgtattg tctcctgcca acggcgaacc
     9421 gattatcgta ccttcccaag acatcgtatt gggcctgtac tacatgaccc gcgaccgtat
     9481 caatgccaaa ggcgagggca gcctgtttgc cgatgtgaaa gaagtgcatc gcgcatacca
     9541 taccaaacag gttgaactgg gtacgaaaat taccgtacgt ctgcgcgaat gggtgaaaaa
     9601 cgaagcaggt gagtttgagc ctgtcgttaa ccgttacgaa acaaccgtcg gccgtgcatt
     9661 gttgagcgaa atcctgccga aaggcctgcc gtttgaatat gtcaacaaag cgttgaagaa
     9721 aaaagaaatt tctaaactga ttaacgcatc gttccgcctg tgcggcttgc gcgatacggt
     9781 tatctttgct gaccacctga tgtacaccgg tttcggattt gcggcaaaag gcggtatttc
     9841 cattgccgtt gacgatatgg aaattccaaa agaaaaagcg gccttgctgg ctgaagccaa
     9901 tgccgaggtt aaagaaatcg aagaccaata ccgtcaaggt ttggttacca acggcgaacg
     9961 ctacaacaag gtggtcgata tttggggtcg tgccggcgat aagattgcta aagcgatgat
    10021 ggacaacttg tccaaacaaa aagttatcga ccgtgacggc aacgaagtcg atcaagagtc
    10081 attcaactcc atttatatga tggcggactc cggtgcccgt ggttctgcag ctcagattaa
    10141 acagttgtcc ggtatgcgtg gcttgatggc aaaacctgac ggctcgatta ttgaaacgcc
    10201 gattacctca aacttccgtg aaggtctgac cgtattgcaa tactttattg cgacccacgg
    10261 tgcgcgtaag ggtttggcgg ataccgcatt gaaaaccgcg aactccggtt acctgactcg
    10321 tcgtctggta gacgtaactc aagatttggt cgttgttgaa gacgattgcg gtacttcaga
    10381 cggctttgtc atgaaggcag tggtacaagg cggtgatgtg attgaagcat tgcgcgatcg
    10441 tattttgggt cgtgttaccg cgtctgacgt tgtcgatccg tcaagtggcg aaaccttggt
    10501 tgaagccggt acgttgctga ctgaaaaact ggtggatatg atcgaccaat ccggtgtcga
    10561 tgaagtcaaa gtccgtacgc cgattacttg taaaacccgt cacggcctgt gtgcacactg
    10621 ttacggtcgt gacttggcac gcggcaaact ggttaacgcc ggtgaggcag tcggtgtgat
    10681 tgctgcacaa tccattggcg aaccgggtac ccagttgacc atgcgtacgt tccacatcgg
    10741 tggtgcggca tcccgtgcgg cagcagccag ccaagtggaa gccaaatcca acggtacggc
    10801 acgattcagc agccagatgc gctacgttgc caacaacaaa ggcgagttgg ttgtcatcgg
    10861 ccgttcttgt gaagtcgtga ttcacgacga tatcggccgt gaacgcgaac gccacaaagt
    10921 accttacggt gccatcctgc tggtacaaga cggtatggcc attaaagccg gtcaaacctt
    10981 ggcaacctgg gatccgcata cccgtccgat gattaccgaa cacgcaggta tggtgaaatt
    11041 cgaaaacgtg gaagagggcg ttaccgttgc caaacaaacc gatgatgtaa ccggtttgtc
    11101 cactttggtg gtgattgacg gtaaacgtcg ttcctctagt gcttccaaac tgctgcgtcc
    11161 gactgtgaaa ctcttggacg aaaacggcgt ggaaatctgt attcccggta cgtccactcc
    11221 ggtttcgatg gcattccccg ttggtgcggt gattaccgta cgcgaaggtc aggaaatcgg
    11281 taaaggcgac gtattggcgc gtatcccgca agcctcttcc aaaacccgcg acattaccgg
    11341 tggtctgccg cgcgttgccg aattgtttga agcacgcgtg ccgaaagatg ccggtatgtt
    11401 ggcggaaatt accggtaccg tttccttcgg caaagagacc aaaggcaagc aacgtctgat
    11461 tgttactgac gtggacggtg tagcatacga gaccttgatt tccaaagaga aacaaattct
    11521 ggtacacgac ggtcaagtgg taaaccgcgg tgaaaccatc gtggacggag ctgttgatcc
    11581 gcatgatatt ctgcgtttgc aaggtatcga agcactggca cgctacattg tccaagaggt
    11641 gcaagaggtt taccgtctgc aaggtgtgaa gatttctgat aaacacatcg aagtcatcat
    11701 ccgtcaaatg ttgcgccgtg tgaacattgc ggatgccggc gaaaccgggt tcattaccgg
    11761 agagcaggtc gaacgcggcg atgtgatggc ggccaatgaa aaagctttgg aagaaggcaa
    11821 agagccggca cgttacgaaa acgtattgct gggtattacc aaagcctccc tgtccaccga
    11881 cagtttcatt tctgccgcat cgttccaaga aacgacccgt gttctgaccg aagccgcgat
    11941 tatgggcaaa caagacgaac tgcgcggttt aaaagagaac gtcatcgtcg gccgcctgat
    12001 tcctgccggt accggtttga cttatcaccg cagccgtcat cagcaatggc aaggggtaga
    12061 gcaggagact gctgaaaccc aagtaacgga tgaataatct tcggcgcgtt tattcaatac
    12121 aaaaccgcaa gctttaagct tgcggttttt ctttgtccga ttaaggcaaa aacaagcgtt
    12181 ttcgtcattt tgaggcgtgt ggattattcc ttaggtattt tcgggccgga gaccaacgag
    12241 gtggcgggtg tcgtcggtac gtccggagac caaaataact ttgccaagga tgttggtttc
    12301 ggcggtcaaa aaagtagcgt cttaatgttt tccatttaaa caaatgtcgt ctgaaacttc
    12361 agacggcatt tcctttaaaa aataaatatg aaacccagaa atctcttttt tgcaggctgc
    12421 ctgctgactt cggcgacgtt tgccgaggat atcggcgtac ctgtcgaact gattaacgtc
    12481 ggtaatcgga ttgcgatgcc gtctgaaggg gaaagcctcg ccctcctgcc gtttgccgag
    12541 gatgtaccgc cggttcgcga tgcaatgccg tctgaagttc ctaaaagcgc ggcaggcggc
    12601 gatgttcggg gtgaccggat gggaatgccg attaacatcg gatgagcgcg gctttatggc
    12661 ataaaaaact gtcgtggaaa ggatttacac cccaaataaa tttccgttac aacaagatca
    12721 acagcaatat gcccgccttt tattcgcgca gcagcaagga atggtttgtc agtatagaaa
    12781 aaacgtattg acagtatttt ctccagccgt ccgactgatt gtgagggatg tcggtaaata
    12841 tttatcggca aacaagaaaa tcatctttct tcttgtcgtt atgcttgact gtatacttgc
    12901 aataaaaata taattccact cttgccgaca tggtgtcggc aagtatttaa ctcaacagga
    12961 cgagaaaata tgccaactat caaccaattg gtacgcaaag gccgtcaaaa gcccgtgtac
    13021 gtaaacaaag tgcccgcact ggaagcctgc ccgcaaaaac gcggcgtgtg cacccgtgta
    13081 tacacgacta cccctaaaaa acctaactct gcattgcgta aagtatgtaa agtccgcctg
    13141 accaacggtt ttgaagtcat ttcatacatc ggcggcgaag gccacaacct gcaagagcac
    13201 agtgtcgtat tgattcgcgg cggtcgtgta aaagacttgc caggtgtgcg ttaccacact
    13261 gtacgcggtt ctttggatac tgcaggtgtt aaagaccgta aacaagcccg ttccaaatac
    13321 ggtgctaagc gtcctaaata attactggga cttaaatagg cacgtcggcc gcctaagctg
    13381 aacaacggcc gagtaagtga atactcaatt gggtattcat gggaatagac ccgactgaat
    13441 agattaaagg aaattaaaat gccaagacgt agagaagtcc ccaagcgcga cgtactgcca
    13501 gatcctaaat tcggcagcgt cgagttgacc aaattcatga acgtattgat gattgacggt
    13561 aaaaaatccg ttgccgagcg tatcgtttac ggtgcgttgg aacagattga gaaaaaaacc
    13621 ggcaaagtag caatcgaagt atttaacgaa gccattgcaa acgccaaacc tatcgtggaa
    13681 gtgaaaagcc gccgtgtagg tggtgcaaac taccaagttc ctgttgaagt tcgtccttca
    13741 cgccgtttgg ctttggcaat gcgctgggtt cgcgatgcgg cccgcaaacg tggtgagaaa
    13801 tccatggacc tgcgtttggc aggcgaattg attgatgcgt ccgaaggccg tggcggtgcg
    13861 ttgaaaaaac gtgaagaagt acaccgtatg gctgaagcca acaaagcatt ctctcacttc
    13921 cgtttctaat tttgaaaggc taataaaatg gctcgtaaga ccccgatcag cctgtaccgt
    13981 aacatcggta tttccgccca tatcgatgcg ggtaaaacca cgacgacaga acgtattttg
    14041 ttctataccg gtttgaccca caagctgggc gaagtgcatg acggtgcggc tactaccgac
    14101 tacatggaac aagagcaaga gcgcggtatt accattacct ccgctgccgt tacttcctac
    14161 tggtccggta tggcgaaaca attccccgag caccgcttca acatcatcga caccccggga
    14221 cacgttgact ttaccgtaga ggtagagcgt tctatgcgtg tattggacgg cgcggtaatg
    14281 gtttactgcg cggtgggcgg tgttcaaccc caatctgaaa ccgtatggcg gcaagccaac
    14341 aaataccaag tgccgcgctt ggcgtttgtc aataaaatgg accgtcaggg tgccaacttc
    14401 ttccgcgttg tcgagcaaat gaaaatccgt ttgcgcgcaa accctgtacc tatcgtcatt
    14461 ccggttggtg cggaagacaa cttcagcggt gtggttgatt tgttgaaaat gaaatccatc
    14521 atttggaatg aagccgataa aggtacaacc tttacctatg gcgatattcc tgccgaattg
    14581 gtcgaaactg ccgaagaatg gcgtcaaaat atgattgaag ccgcagccga agccagcgaa
    14641 gaactgatgg acaaatactt aggcggcgac gagctgaccg aagaagaaat cgtaggcgcg
    14701 ttgcgtcaac gtactttggc aggcgaaatt cagcctatgc tgtgtggttc tgcatttaaa
    14761 aacaaaggtg ttcaacgtat gttggacgca gttgtagaat tgctgccagc tcctaccgat
    14821 attcctccgg ttcaaggtgt caacccgaat accgaggaag ccgacagccg tcaagccagc
    14881 gatgaagaga aattctctgc attggcgttc aaaatgttga acgacaaata cgtcggtcag
    14941 ctgaccttta tccgcgttta ctcaggcgta gtaaaatccg gcgataccgt attgaactcc
    15001 gtaaaaggca ctcgcgaacg tatcggtcgt ttggtacaaa tgactgccgc agaccgtact
    15061 gaaatcgaag aagtacgcgc cggcgacatc gcagccgcta ttggtctgaa agacgttact
    15121 accggtgaaa ccttgtgtgc ggaaagcgcg ccgattatct tggaacgtat ggaattcccc
    15181 gagccggtaa tccatattgc cgttgagccg aaaaccaaag ccgaccaaga gaaaatgggt
    15241 atcgccctga accgcttggc taaagaagac ccttctttcc gtgtccgtac agacgaagaa
    15301 tccggtcaaa ccattatttc cggtatgggt gagctgcact tggaaattat tgttgaccgt
    15361 atgaaacgcg aattcggtgt ggaagcaaat atcggtgcgc ctcaagtggc ttaccgtgaa
    15421 actatccgca aagccgttaa agccgaatac aaacatgcaa aacaatccgg tggtaaaggt
    15481 caatacggtc acgttgtgat tgaaatggaa cctatggaac cgggtggtga aggttacgag
    15541 tttatcgatg aaattaaagg tggtgtgatt cctcgcgaat ttattccgtc tgtcgataaa
    15601 ggtatccgcg atacgttgcc taacggtatc gttgccggct atcctgtagt tgacgtacgt
    15661 atccgtctgg tattcggttc ttaccatgat gtcgactctt cccaattggc atttgaattg
    15721 gctgcttctc aagcgtttaa agaaggtatg cgtcaagcat ctcccgccct gcttgagccg
    15781 attatggcag ttgaagtgga aaccccggaa gaatacatgg gcgacgtaat gggcgacttg
    15841 aaccgccgtc gcggtgttgt attgggtatg gatgatgacg gtatcggcgg taaaaaagtc
    15901 cgtgccgaag tacctctggc agaaatgttc ggttattcga ccgacctgcg ttctgcaacc
    15961 caaggccgcg ctacttactc tatggagttc aagaaatatt ctgaagctcc tgcccacata
    16021 gctgctgctg taactgaagc ccgtaaaggc taatcaggca aataggccgt ccgaaaggct
    16081 gaaatgattt ttcagacggc attgttcttt aatcgatctt taatgtaaag gaattagc
//
LOCUS       DAWKOG010000031        16118 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.031, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000031 DAWKOG010000000
VERSION     DAWKOG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16118
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.031"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(345..767)
                     /locus_tag="WJK66_000764"
     CDS             complement(345..767)
                     /locus_tag="WJK66_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEZ7578508.1"
                     /translation="MQVTSKWIDGMCFVGTTEGGHSVVMEGSAAEGAAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(885..1556)
                     /locus_tag="WJK66_000765"
     CDS             complement(885..1556)
                     /locus_tag="WJK66_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEZ7578509.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDAVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QAAKP"
     gene            complement(1572..2198)
                     /locus_tag="WJK66_000766"
     CDS             complement(1572..2198)
                     /locus_tag="WJK66_000766"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEZ7578510.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AGQGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSVLPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGIVPILDGDTA
                     DDVAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(2305..3123)
                     /locus_tag="WJK66_000767"
     CDS             complement(2305..3123)
                     /locus_tag="WJK66_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ7578511.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASSAQGDT
                     SSIGSTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGE
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEA
                     TFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(3382..3822)
                     /locus_tag="WJK66_000768"
     CDS             complement(3382..3822)
                     /locus_tag="WJK66_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEZ7578512.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGQLQEL
                     DKMLWQYEAESFIPHEIWETEEAMPSEPSVLLACGGNLPRIPEGMAVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEELADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(3885..5291)
                     /locus_tag="WJK66_000769"
     CDS             complement(3885..5291)
                     /locus_tag="WJK66_000769"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEZ7578513.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     NFADTKIPTGNGLQAVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNVDVHAFEEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFAEAVFHSAHEAAVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSVED
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAVVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLVDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEGYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            5461..6576
                     /gene="lptF"
                     /locus_tag="WJK66_000770"
     CDS             5461..6576
                     /gene="lptF"
                     /locus_tag="WJK66_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEZ7578514.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGGFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLDLRNGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            6573..7643
                     /gene="lptG"
                     /locus_tag="WJK66_000771"
     CDS             6573..7643
                     /gene="lptG"
                     /locus_tag="WJK66_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEZ7578515.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGLIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGIWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            7953..10538
                     /gene="acnB"
                     /locus_tag="WJK66_000772"
     CDS             7953..10538
                     /gene="acnB"
                     /locus_tag="WJK66_000772"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEZ7578516.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFTSRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            10680..11675
                     /locus_tag="WJK66_000773"
     CDS             10680..11675
                     /locus_tag="WJK66_000773"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEZ7578517.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFGQETVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEVFESPASIVFDQAENRMHTIKAVMVAALGD"
     gene            complement(11744..11845)
                     /locus_tag="WJK66_000774"
                     /pseudo
     CDS             complement(11744..11845)
                     /locus_tag="WJK66_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(11899..12912)
                     /gene="ilvC"
                     /locus_tag="WJK66_000775"
     CDS             complement(11899..12912)
                     /gene="ilvC"
                     /locus_tag="WJK66_000775"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEZ7578518.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVAANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
     gene            complement(12992..13285)
                     /locus_tag="WJK66_000776"
     CDS             complement(12992..13285)
                     /locus_tag="WJK66_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEZ7578519.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAA"
     gene            complement(13352..13843)
                     /gene="ilvN"
                     /locus_tag="WJK66_000777"
     CDS             complement(13352..13843)
                     /gene="ilvN"
                     /locus_tag="WJK66_000777"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEZ7578520.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            complement(13854..15581)
                     /gene="ilvB"
                     /locus_tag="WJK66_000778"
     CDS             complement(13854..15581)
                     /gene="ilvB"
                     /locus_tag="WJK66_000778"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEZ7578521.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNVLSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADGKA"
BASE COUNT         3779 a         4384 c         4084 g         3871 t
ORIGIN      
        1 caacgccgta ctggtttttg ttaatccact ataattcaat aaattaatat atggcttaaa
       61 ataacgggat tctcgcctcc cgcccgcccg cagaagcagg cgggtatcat tttaaaacgc
      121 ggcatttaaa atttgaccga aaattgttga caatccggaa tcaagtctgc acaatacccc
      181 gacaagtcca agtattataa aggctgaata aagaggaaac agcaggcaga tatattcggg
      241 aggtgcagtc cgaatatatc tgctttttta tgcgcctccg gattgcctgc cgcacctttc
      301 ccttcagacg gcatcagccg tttccccata atgccgcccg atgcctattt atctgccccg
      361 gcaatttcaa aactgtgggt aatctttgcc gctttgccca acataatcga agccgaacag
      421 tatttttcgg cagacatctg aacggcgcgc tcaatggccg attctttcaa atcatgcccg
      481 aatactttga aatggatgtg gatttcggta aacacgcgcg gcgcatcgtc cgcccgtttc
      541 gccgtaaccg tcgcacggca gtcagtcact ttctgacgct gtttttcggc aatcatcacc
      601 acatcgatgc tcgaacagcc cgccacgccc aacagcagca tttccaaagg gctgggcccg
      661 cgcttggccg caccttctgc cgccgacccc tccataacga cgctgtgccc gccttccgtt
      721 gttccgacaa agcacattcc gtctatccat tttgatgtaa cctgcatggt gtcattcctg
      781 aaaatagcgt taaaaccgct ttgcatatgg cgttattgta aacaatttca agcggcttat
      841 gcagaaatat ggacaaaacg gcaaaaaaac gcctgaaaat cgatttacgg tttggctgcc
      901 tggccgttga tctgcaccga tttgagtttc agcgtatagg ttttgccgtc gtcggtatag
      961 ccgatttgtg ccggaatatt gttcagggac ggtgcgaaga aatacattac cgcatcgtcg
     1021 ccgcgccgca cccgatattt gacgacttcg gtttccacgc cgcctatgct gtattttcct
     1081 gtacccgcct tattcaaacc gccgacggaa taaagttttt tgccgttggt gattttcagc
     1141 cccgggggga gtttcgcgtc atttgccgcc aactgccagg caagcgtgaa caaatccata
     1201 gccttggggc tttgctcggt tttgctctcg cccgctttgc cgtaggttac gctgccgtcg
     1261 gcgaatttgg cttccgcata cagtttgccc ctgcgtatgt ctctatagta ggtagggtgc
     1321 agggtattgc cgacaaccgt accgccggac tcgaaacgga tattgtatag cggcacttta
     1381 atcgtcgaaa cgattttgta agcattgccg ctgcgttcaa atgtcatcgt ggcgggaatg
     1441 ccgtagctgc cggaatagtg cagcacggcg gattggggca gccctgccgc atacgcgcac
     1501 ggcagggcgg cggacaaaat ggcggcggaa aatatatttt taaaagtctt catcatttgc
     1561 tcccgcccgg tttacgccgt caggaaacgg gcggcatcgg cgttttccga atttctgacg
     1621 cggtttccct cgataatcag gcggccggcg gcaaaatcgg caacggcttt cggataaagt
     1681 ttatgctcaa cagccaaaac ccgggcggca acatcgtctg ccgtatcgcc gtcgagtatc
     1741 ggcacaatcc cttgcgatac aatcgggccg caatccagtt cggcagtaac gaaatggatg
     1801 gtgcagccgg caacgcggca gcccgcctcc aaagcgcgtt cgtgcgtatg aagtccggta
     1861 aacgagggca ggacggacgg gtgaatgttc atcagcctgc cttcgtaatg ggcacagaac
     1921 tcaggggtca gaatccgcat aaaacctgcc aaaaccacca agtcgggttg atatgcgtcg
     1981 attttttcca tcatggcggt atcgaaggca agtcgtgatt caaagttttt atgattcagg
     2041 ctgtcggtcg gaataccctg accggccgcc cattgcaaac cggcagccgt ttcgctgttg
     2101 ctcaacacgg cggcaatacg gacgttggga atggcggcat tgacgattgc ctgcatattg
     2161 ctgccgcgtc cggaaatcag gatgacgatg tttttcataa tggtgcgctt ttgaaaggga
     2221 tgccatctga accgctgttt ggtggtttca gacggcattt gccgtaaaaa tgcccgaaaa
     2281 cctgtttcgg gcatggattc ggacttaatt tacttttttg atgtcgactt gagccggctg
     2341 cttggcgggc gcgttttcgg gtgcgccgat tttgaccagt ttcacatcaa ataccaaagt
     2401 ggcgttcggg ccgattttgt cgcccgcacc ctgttcgcgg taggcaaggt tggacgggat
     2461 gtagaacgtg gcttcgccgc cttctttcag aagctgtacg ccttcggtcc aacccggaat
     2521 cacttggctc aaagggaagg tgaccgggcc gccgttggct ttgctgctgt cgaataccgt
     2581 accgtcaatc aggcggcctt cgtattccac ggtaacgatg tcgtctttgg tcggctgttt
     2641 gccttcgccc tgtttggtga ttttgtattg caggccggaa gcagtggtct tcacgccgtc
     2701 tttggcggca ttttctttca gaaaggcttc gcctttttct ttattggcct tcgcgtccgc
     2761 cttgtgtttt tctacggctt tagcctgttg ttcctgaagg aatttcatca tgacttcctg
     2821 agcctgctct tcggtcattt tgatttcttt gccgtcatac actgcctgca tggcttcggt
     2881 aaagactttc aaatcgattt ccgcgccctg ttccttcatt tgcttcaggg agcgtccgat
     2941 gtccacgccc atcgcatagc ttgcctgctg catcgtgctg ccgatcgaag aggtgtcgcc
     3001 ctgcgcggaa gaagcggcgg caggttcgga tgcagatgcg ggggcggctt cttttttgcc
     3061 gcaggcggaa agtgccaaag cggcggaaag ggtcagtgcg ctgattttga aaatggtgtt
     3121 catgatggat cttcgctgtc gataaggtcg gaaaaacggg attatagccg agtttgaatg
     3181 tttcaacaca caggatgaca cataaagcgt caatcgtgtg ttgccctgtt ttggaaaaga
     3241 ttgaaccttc caaaataagt tttgattcta ccgccccgag ggacagatgt ccaagtggcg
     3301 gggttcaacc gataaggaaa ttttaatcaa atagaatcaa gcctgtttaa attttgtaaa
     3361 tgcggcattt cagacggcat tttatgcctt gccctccatg ccgtgatgtt cgatggcaaa
     3421 accgcttcgg cggtaggcgg taaagcgttc gcgcgcgtcg gcgagctctt ccaggctgtt
     3481 gccgacgatt tctaaaacgc gcgcgggcaa gaccggagcg gtgttccaaa aaccgtcgga
     3541 caggttcaaa acggccatgc cttcgggaat tcggggcaga ttgccgccgc aggcaagcag
     3601 gacggatggt tcagacggca tggcttcttc cgtttcccaa atttcgtgcg gaataaaact
     3661 ctcggcctcg tattgccaaa gcattttgtc caattcctga agctgcccga acgaatcgga
     3721 ccacaccagt atcctgccgc cgtcccgaat cgcacgggca atcaggcggc aggtgaaaat
     3781 cggaacttgg gcaacgtgcg tgtaaaaggt ggctttcggc atattgtttg agcatttggc
     3841 aggacgatgc cgtctgaaag gcttcagacg gcattgtggc aaaattaaag attccgcaga
     3901 tagttcagca gcaagggaac gggacggcca gtcgcacctt tttccgctcc ggatttccac
     3961 gccgtacccg cgatgtcaag gtgcgcccac gggtagcctt cggtgaagta agacaggaat
     4021 gttgcggcgg taatcgtgcc cgcgccgggc gtgccgatat tggggatgtc ggcaaagttg
     4081 gatttgagct ggtctttgta ggtttcaaag agcggcagtt gccacgcttt gtcgtccacg
     4141 ttgtaggaag cggcaagcag gctgtcgacc aaatcttgat tgttgcccat cacgccgctg
     4201 acatcgtgcc ccaaggcgac aatacacgcg ccggtcaggg tggcgacgtc gatgacggct
     4261 ttgggtttga actgttcggc gtaagtgagc gcgtcgcaca aaatcagacg gccttcggca
     4321 tcggtgttca acacttcgat ggtcagacct ttcatacttt tcacgacatc gcccggtttg
     4381 tttgccgcgc cggaaggcat attttcacaa gtggcgacga cggcaatcag gttaatcggc
     4441 agttgcagtt tgacggcggc gcagaaggtg ctgatgacgg ttgccgcgcc gcacatatca
     4501 aacttcattt cgtccatgtt caggccgggt ttgagggaaa tgccgccggt gtcgaaggta
     4561 atgcctttgc cgaccaatac cacaggcgcg gcttctttgt ccgccgcacc gaaatagctc
     4621 agttcgacca aatagggatc ttcgacactg cctttggcga ccgaccaaaa cgaacccatg
     4681 ttttctttga tgtagtcttt ttcaatgatt ttggcgtgtg cgcccaattt ttcggcttcg
     4741 gctttggcgg tgcgcgctaa aaattcgggc gtgcattcgt tgggcgcggc gttgcccaag
     4801 tcgcggcaga ggctttgtcc gtaaacctgc gcttcggcaa cgcgcagggc ttctttgacg
     4861 gcggcttcgt gtgcgctgtg gaacacggct tctgcaaatt tggcgggctt ggcttctttt
     4921 ttatagcggt cgaaacggta ggcggcattg ccgaaagcaa tcgcaaacgc ttcggcaacg
     4981 gctgcggctt gcgcttcttc aaaggcatga acgtccacat tgaccgtttc ctgattttgc
     5041 gcccatttgg cggcttcggc agcggccttg ttcaatgcgg cgcggccggt gcttttcaga
     5101 cagcatacgg caacagcctg caaaccgttg cctgtcggga tttttgtgtc ggcaaaattt
     5161 tgaccttctt caagcgaaga caaaagggca agggcggtcg gatggtttaa ttgcgcctct
     5221 tcggtgcaga caaataactg tgcgcctgcc tgctgttcct gcaagatttc ggtttttgtg
     5281 ctaaattcca cgtttattct cctgattgag acggttgtcg gtagttttcg gacggccttt
     5341 cgctcaaaag accgtctgaa gacggctggc acgattgtac cccatttgaa gcaccgtctg
     5401 aaaccttgcg cggacaatcc gcctgcgccg aaccgcttac cgccccctga ccgcgattct
     5461 atgatttatc aaagaaacct catcaaagaa ctctctttta ccgccgtcgg cattttcgtc
     5521 gtcctcttgg cggtattggt ctccacgcag gcaatcaacc tgctcggccg tgccgccgac
     5581 gggcgtgtcg ccatcgatgc cgtgttggca ttggtcggct tctgggtcat cggtatgacg
     5641 ccgcttttgc tggtgttgac cgcatttatc agtacgttga ccgtgttgac ccgctactgg
     5701 cgcgacagcg aaatgtcggt ctggctatcc tgcggattgg cattgaaaca atggatacgc
     5761 ccggtgatgc agtttgccgt gccgtttgcc gttttggttg ccgtcatgca gctttgggtg
     5821 ataccgtggg cagagctacg cagccgcgaa tacgctgaaa tcctgaagca gaagcaggaa
     5881 ttgtctttgg tggaggcagg cgggttcaac agcttgggca agcgcaacgg cagggtttat
     5941 tttgtcgaaa ccttcgacac tgaatccggc attatgaaaa acctgttcct gcgcgaacag
     6001 gacaaaaacg gcggcgacaa cattatcttc gccaaagaag gcaatttctc gctgaacgac
     6061 aacaaacgca cgctcgacct gcgcaacggc taccgttaca gcggtacgcc tggacgcgcc
     6121 gactacaatc aggtttcctt ccaaaaactc aacctgatta tcagcaccac gcccaaactc
     6181 atcgaccccg tttcccaccg ccgtaccatt ccgaccgccc aactgattgg cagcagcaac
     6241 ccgcaacatc aggcggaatt gatgtggcgc atctcgctga ccgtcagcgt cctcctactc
     6301 tgcctgcttg ccgtgccgct ttcctatttc aacccgcgca gcggacatac ctacaatatc
     6361 ttgattgcca tcggtttgtt tttaatttac caaaacgggc tgaccctgct ttttgaagcc
     6421 gtggaagacg gcaaaatcca tttttggctc ggactgctgc ctatgcacat catcatgttc
     6481 gtcatcgcaa tcgtacttct gcgcgtccgc agcatgccca gccagccctt ctggcaggct
     6541 gttggcaaaa gtctgacatt gaaaggcgga aaatgaacct gatttcacgt tacatcatcc
     6601 gtcaaatggc ggttatggcg gtttacgcgc tccttgcctt cctcgctttg tacagctttt
     6661 ttgaaatcct gtacgaaacc ggcaacctcg gcaaaggcag ttacggcata tgggaaatgc
     6721 tgggctacac cgccctcaaa atgcccgccc gcgcctacga actgatgccc ctcgccgtcc
     6781 ttatcggcgg actggtctct ctcagccagc ttgccgccgg cagcgaactg accgtcatca
     6841 aagccagcgg catgagcacc aaaaagctgc tgttgattct gtcgcagttc ggtttgattt
     6901 tcgccattgc caccgtcgcg ctcggcgaat gggttgcccc cacgctgagc caaaaagccg
     6961 aaaacatcaa agccgccgct attaacggca aaatcagcac cggcaatacc ggcatttggc
     7021 tcaaagaaaa aaacagcatt atcaacgtgc gcgaaatgtt gcccgaccat accctgctgg
     7081 gcattaaaat ctgggcccgc aacgataaaa acgaactggc agaggcagtg gaagccgatt
     7141 ccgccgtttt gaacagcgac ggcagttggc agttgaaaaa catccgccgc agcacgcttg
     7201 gcgaagacaa agtcgaggtc tctattgcgg ctgaagaaaa ctggccgatt tccgtcaaac
     7261 gcaacctgat ggacgtattg ctcgtcaaac ccgaccaaat gtccgtcggc gaactgacca
     7321 cctacatccg ccacctccaa aacaacagcc aaaacacccg aatctacgcc atcgcatggt
     7381 ggcgcaaatt ggtttacccc gccgcagcct gggtgatggc gctcgtcgcc tttgccttta
     7441 ccccgcaaac cacccgccac ggcaatatgg gcttaaaact cttcggcggc atctgtctcg
     7501 gattgctgtt ccaccttgcc ggacggctct tcgggtttac cagccaactc tacggcatcc
     7561 cgcccttcct cgccggcgca ctacctacca tagccttcgc cttgctcgcc gtttggctga
     7621 tacgcaaaca ggaaaaacgc taaaccaatg ccgtctgacc cccttcggac ggcattggtt
     7681 tttattatca tattcctgta gacagatagt cgttccctat tatagtggat taacaaaaac
     7741 cagtacggcg ttgcctcgcc ttgccgtact attgtactgt ctgcggcttc gtcgccttgt
     7801 cctgattttt gttaatccac tatacattac ctgtcataac agttccattt ttgttaaaac
     7861 tagtctatga tagcggtaca aatattgttt acaatattta acgcaaatca tttgcaaccc
     7921 gacaaaagaa aaacagaaaa aggaacaaag agatgttaga agcctatcgt aaagccgccg
     7981 ccgagcgcgc cgccctcggc attcccgccc tccctttgaa cgcgcagcaa accgccgatt
     8041 tggttgagct gctgaaaagc ccgcccgcag gcgaaggcga gttcttggtc gaactgcttg
     8101 cccaccgtgt tccgcccggt gtggacgatg ccgccaaagt caaagcctca ttcctggctg
     8161 ccgttgccga aggcagcgcg tccagcccgc tgatctcccc cgaatatgcg accgaactct
     8221 taggtacgat gctcggcggt tacaatatcc acgccttaat cgaactcttg gacgacgaca
     8281 aactcgcgcc cattgccgcc aaaggcttga aacatacgct tctgatgttc gattccttcc
     8341 acgacgttca ggaaaaagcc gaaaaaggca acaaatacgc gcaagaagtt ttgcaatctt
     8401 gggcagatgc cgaatggttt acctcacgcg ccaaagttcc cgaaaaaatc accgttaccg
     8461 ttttcaaagt tgacggcgaa accaatacag acgacctctc ccccgcgccc gacgcgtgga
     8521 gtcgtcccga tattccgctg cacgcgctgg ccatgctgaa aaacccgcgc gacggcatca
     8581 cgcccgacaa accgggcgaa gtcggtccga ttaaattgtt ggaagaactc aaagccaaag
     8641 gccatccggt tgcttacgtc ggcgacgtgg tcggtaccgg ttcttcacgc aaatccgcga
     8701 ccaactccgt catttggcat accggcgaag acattccgtt cgtgccgaac aaacgcttcg
     8761 gcggcgtatg tttgggcggc aaaatcgcgc cgattttctt caatacccaa gaagattccg
     8821 gcgcgctgcc gattgaagtc gatgtatctg ctctaaaaat gggtgatgtc gtcgatatcc
     8881 tgccttatga aggcaaaatc gtgaaaaacg gcgagactgt tgccgagttt gaattgaaat
     8941 cacaagtatt gctggacgaa gtgcaagccg gcggccgtat caacctgatt atcggccgag
     9001 gtctgaccgc caaagcgcgc gaagccctga aactgcctgc ctctactgca ttccgcctgc
     9061 cgcaagcgcc tgccgaaagc aaagccggtt tcaccttggc gcaaaaaatg gtcggccgcg
     9121 cctgcggtct gcccgaagga caaggcgtgc gcccgggtac ttactgcgaa ccgcgtatga
     9181 cgacggtcgg ctcgcaagac acgaccggcc cgatgacccg cgacgagttg aaagacttgg
     9241 cttgtttggg cttctccgcc gatatggtga tgcagtcttt ctgccacacc gccgcctatc
     9301 cgaaacctgt cgatgtaaaa acccataaag aactgcccgc ctttatttcc acccgtggcg
     9361 gcgtgtcact gcgtccgggc gacggcgtca tccactcgtg gctcaaccgc ctgctgctgc
     9421 ccgataccgt cggcaccggc ggcgacagcc atacccgttt ccccatcggt atttccttcc
     9481 ccgccggctc cggcttggtt gcctttgccg ccgcaacggg cgtaatgccg ctcgatatgc
     9541 ccgagtctgt attggtacgc ttcagcggca agctgcaacc gggcgtaacc ctgcgcgatt
     9601 tggtgaacgc catcccgctg tacgcaatca aacaaggttt gctgaccgtt gccaaagccg
     9661 gtaagaaaaa catcttctcc ggccgcatcc tcgaaatcga aggcctgcct gatttgaaag
     9721 tggaacaagc ctttgaattg accgacgcat ccgccgaacg ctccgccgcc ggctgtaccg
     9781 tgaagctcaa caaagagccg attatcgagt acatgaaatc caacgtcgtg ttgatgaaaa
     9841 acatgattgc caacggctat caagacccgc gcactttgga acgccgcatc aaagctatgg
     9901 aaaaatggct ggcaaatccc gagttgctcg aagcggataa agatgccgaa tacgccgccg
     9961 tgattgaaat caacatggac gacatcaaag agccgattat cgcctgcccg aacgacccgg
    10021 acgacgtgtg cttcatgtcc gaacgctccg gcaccaaaat cgacgaagta ttcatcggtt
    10081 cgtgtatgac caacatcggc cacttccgcg ccgcctccaa acttttggaa ggcaaggcag
    10141 acacccccgt ccgcctgtgg attgcgccgc cgaccaaaat ggacgcgaaa caattgtccg
    10201 acgaaggaca ctacggcgta ctcggacgtg ccggcgcgcg tatggaaatg ccgggttgct
    10261 ccttatgtat gggtaatcag gcgcaagtac gcgaaggtgc gaccgttatg tccacctcca
    10321 cccgcaactt cccgaaccgt ttgggtaaaa acacctttgt ttacctcggt tcggcggaat
    10381 tggcagcgat ttgctccaaa ctgggtaaaa tcccgaccgt tgaagaatat caagccaata
    10441 tcggcatcat caacgaacag ggcgataaaa tctaccgcta tatgaacttc aacgaaatcg
    10501 acagctacaa cgaagtagcc gagaccgtga acgtttaatc cccgtcatcc gtatgaagta
    10561 agggattgac cgcaatgccg tctgaacaac cttcagacgg cattgcaaca ttccgctaac
    10621 ccttctttcc gcaaacgctg caaatacggc gttcacgccc ccacataaag gaaacgacag
    10681 tgaacctgaa aaaccgccat tttctgaaac ttttagactt cacgccggaa gaaatcaccg
    10741 cctacctcga ccttgccgcc gaattgaaag ccgccaaaaa agcagggcgc gagattcagc
    10801 ggatgaaagg gaaaaacatc gccctgattt ttgaaaaaac ctctactcgg acgcgctgcg
    10861 cgtttgaagt cgccgcgcgc gatcaaggcg cgggagtgac ttatttagag ccgtccgcca
    10921 gccaaatcgg gcataaggaa agcatcaaag acaccgcccg cgtgttgggc aggatgtacg
    10981 atgccatcga atatcgcggt ttcggtcagg aaactgtcga agaattggcg aaatacgcgg
    11041 gcgtacccgt gttcaacggg ctgaccaacg agttccatcc cacacaaatg cttgccgacg
    11101 cactgactat gcgcgaacac agcggcaaac ctttgaacca aaccgcgttt gcctacgtcg
    11161 gcgacgcgcg ttacaacatg ggcaattccc tgctgatttt aggggcaaaa ttggggatgg
    11221 acgtgcgtat cggcgcaccg caaagcctgt ggccgtctga aggcattatt gccgccgcac
    11281 acgccgccgc caaagaaacc ggcgcaaaaa ttaccctgac cgaaaacgcg catgaagccg
    11341 tgaagaatgt tgattttatt cataccgatg tgtgggtcag catgggcgag ccgaaagaag
    11401 tctggcagga acgcatcgat ttgctgaaag attaccgcgt tacgcccgaa ctgatggcgg
    11461 catcgggcaa tccgcaagtc aaattcatgc actgcctgcc cgccttccac aaccgcgaaa
    11521 ccaaagtcgg cgaatggatt tacgaaacct tcgggctgaa cggtgtggaa gttacagaag
    11581 aagtattcga aagccccgcc agcatcgtgt tcgatcaggc ggaaaaccgt atgcacacga
    11641 ttaaagcggt aatggtcgcg gctctgggcg actgacagaa ctgtgcctgt ttaaattcat
    11701 ccgcaacaca gataccgtct gaacacgatg ttcagacggt atccatatat agtggattaa
    11761 atttaaacca gtacggcgtt gcctcgcctt gccgtactat ttgtactgtc tgcagcttcg
    11821 tcgccttgtc ctgatttttg ttaatccact ataaaaaaac tgcctacacg atgtgtaggc
    11881 agtcccgttt gaaaacaatc agtttttgtc ttggtcaacc aatttgttgg cagtaatcca
    11941 aggcatcatg gcacgcagtt gtgcgccgac tttttcaact tggtggtcgg cattcaaacg
    12001 gcggcgggca gtcatagacg cgtagttgac gttaccctct tggataaaca tttttgcgta
    12061 ttcgccggtt tggatgcgtt tcagagcatt gcgcatggct tctttgctgg aagcattgac
    12121 cacttcaggg ccggtaacgt attcgccgta ctccgcattg ttggaaatgg agtagttcat
    12181 attggcaata ccgccttcga aaatcaggtc aacaatcagt ttcatttcgt gcagacattc
    12241 gaagtaagcc atttcaggcg cgtaaccggc ttcggtcagg gtttcaaaac ctgctttgat
    12301 caactcgacc acgccaccgc acaatacagc ttgttcgccg aacagatcgg tttcggtttc
    12361 ttcgcggaaa gtggtttcaa tcacaccgcc tttggtgccg ccgttggcag ccgcataaga
    12421 cagggcgatg tctttggcct tgccggaatt gtcttggtaa acggcaatca gagaaggcac
    12481 gccgccgccg cgtttgtatt cactgcgtac ggtatgaccc gggcctttgg gggcaaccat
    12541 aatcacgtcc aagtcggcac gcggaacgat ttggttgtag tgaacgttga agccgtgtgc
    12601 aaatgccagc gttgcgcctt ctttcagatt ggctgcaact tcggcgtgat agacggcagg
    12661 catggtttcg tcaggcagca gcagcataac gacatcggct tctttggtcg cttcagcaac
    12721 agttttgacg acatggccgg ctgcttcggc ttttttccaa gaagaacctt ggcgcagacc
    12781 aatcaccacg tttacacccg aatctttcag gttggcagca tgggcatggc cttgcgaacc
    12841 gtaaccgatg atggcaacgg ttttgccttt gatcagggac agatcggcat ctttatcgta
    12901 atagacttgc atttgatttc ctttaaggta aatggttgtc gaagccttaa aatgttgagc
    12961 ggcttcggac gggttaaaca gagtgtgccg cttaagcggc aacttcattc atcaatacga
    13021 tttccaacgc ttcggttttg ccgtcgacgg actggacgaa ggcttggaaa tgcgcgctgg
    13081 cattgtgttc gtcaatagct gcttgggatt tccaattttc cacgaaaaca aaacggttcg
    13141 gtttgccgat ttcctgatgg agatcatagc tgatgttgcc ctcttccgca cggctggctt
    13201 tgaccagttc tttaaactgt gctgccagtg tttctgtgta ttccggttta acggtaacca
    13261 gtgcgacaat tttaatgttc gacataaatc tctcctgccg ttcgtttttc agacggcatt
    13321 caaataccgt gccgtctgaa aggttacggc gttaaatttt caaaatacgc tcaccgcgac
    13381 cgatgccggc cgcgcctgta cgtacggttt ccaaaatttg ggcgcgtccg accgtttcca
    13441 aaaaggaatc cagcttgtct gtcgagccgg taatttcaat cgtatagctg cggtcggtta
    13501 cgtcgatgat gctgccccgg tagatttcgg tcaagcgtaa aaattcgtcg cggtctttgc
    13561 cggcggcacg gacttttacc aacatcagtt cgcgttcgac aaaacggctt tcgttcaaat
    13621 cgaccacttt aatcacttca atcaatttat tgagttgctt ggtgatttgt tcgataacct
    13681 gctcgtcgcc gtgtgtaacg atggtcatac gggacagggt tttgtcttcg gtcggcgcaa
    13741 ccgccaaaga atcgatattg taatcgcgtg cagagaacaa accgaccacg cggctcatcg
    13801 cacctgattc gttttcgatc agaacagata agatatgtcg catttgcctc tccttacgcc
    13861 tttccgtccg cacgcatatg cggcggaagt accatttcgt ccaaaccttt gccgttgccg
    13921 accatgggca tcacattctg tttctggtcg gtcaggaagt cgataaacac cagcctgtct
    13981 ttttggttca atgcttccaa caacgcacct tccacatcag acttcttgtc cacgcggata
    14041 ccgatatggc cgtatgcctc ggcaagtttg acgaaatcgg gcaaagaatc gaaataggtt
    14101 tccgactctc gtccgccgta atatatttcc tgccactggc gtaccatacc gagataaccg
    14161 ttgttcagcg taatgacgtt aaccggaatc cgatattgga aacaggtgga cagctcttgg
    14221 atgttcatct ggatcgagcc gtcgccggta atacagaata cgtcttgatc cggggcggca
    14281 agttttgcac caatcgcata aggcagacca acgcccatcg tacccaaacc gccggagttg
    14341 agccattggc gcggacgttc gaagggataa tattgagccg caaacatttg atgctgccct
    14401 acatccgatg tgatgattgc cgaattgccg gtaatctcgg caagcttctg aatcacatat
    14461 tgtggcttga taatttcgct gccgttgtca aaccacaagc aatcgcggga acgccattcc
    14521 tctatggttt tccaccattt gcccaaagca tcttcagacg gcacggactc ttgtttttgc
    14581 cacagcgcaa ccatctcgga caaaacgttt ttcacgtcgc cgacaatcgg aatgtccgcc
    14641 ttcacgcgtt tggcgatgct ggaaggatcg acatcgatat ggataacctt cttcgccttc
    14701 tcgaaaaatt tggacggtac ggaaaccaca cggtcgtcaa aacgcgcacc tacggcaaga
    14761 acgacatccg cattctgcat ggcaaggttt gcctcgtaag taccgtgcat accgagcata
    14821 ccgaggaatt ggcggtcgcc ggaaggataa gcgcccaagc ccatcagcgt acccgtgcac
    14881 ggagcacccg tcattcggac aaatcgggtc agctcttcag aagcattacc caacaccacg
    14941 ccgccgccaa aatagacgac cggacgtttg gcagatgcca acatctgcac ggccttttta
    15001 atctgaccga tatgcccttg aacaaccggt tgatacgaac ggataaaaat gtcttcctga
    15061 ggatagctga atttcgccat cgcctgcgta acatctttcg ggacatcaac caccacgggc
    15121 cccggtcggc cgcttgcggc aatttggaac gcctttttaa tggtttccgc caactcattg
    15181 atgtccgtaa ccaggaaatt gtgtttgacg cacggacggg taatacccac cgtatcaact
    15241 tcttggaacg catccgtacc aatcagggaa ttgcctacct gcccgctgat gaccaccatc
    15301 ggaatcgaat ccgtataggc agtagcaata ccggtcagtg cattggtaac gcccgggccg
    15361 gatgtaacca atgccacgcc caccttaccg ctgacgcgcg catacgcatc tgccgcgtgt
    15421 actgccgcct gctcgtgacg ggtcagaatg tgcttgaatt tattgagttg gaaaagggca
    15481 tcgtagattt cgataaccgc accgccggga taaccgaaaa cgtactcgac accttcggct
    15541 ttgagactct gcactatgat ttgcgcgcct gatagctgca taacgacctc ttttatacgg
    15601 tttcaaacca atagggacaa accgctttgc cacagcacct gtaatgcaat tccaccaagc
    15661 agcgatttag ggtacgcgca ttgggggaac acggcaacag acggattatc caatcaattg
    15721 gaaaggaaca cagagtttgt gaaaaagagt agaaacgata acgcaaaccg acagttcaat
    15781 caagaaaaat ctttcatctt ttaattattt tttgaaagca gagaaattat tgattgattt
    15841 taaaagaata aaatcaggag tacctttttt gaaagatgga aattgttgac agtttgtgta
    15901 ggaggggcag atgtgaaaaa cccttcttcg atatcaagaa ttgtaaaatt tacagggttt
    15961 catcccaata aagactcggg atattgattg aacttgattt tatttttgat atatcaaaaa
    16021 tattcccaac catacttcct gaaaatggct cattgcaccg gactgtattg gacggcatta
    16081 acagaaccaa gagggctaac aacgacttaa tatattga
//
LOCUS       DAWKOG010000032        15363 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.032, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000032 DAWKOG010000000
VERSION     DAWKOG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15363
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.032"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(104..1888)
                     /gene="lpdA"
                     /locus_tag="WJK66_000779"
     CDS             complement(104..1888)
                     /gene="lpdA"
                     /locus_tag="WJK66_000779"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ7578522.1"
                     /translation="MALVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGAAAAPKAEAAAAPAQEAPKAAA
                     PAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKNGVVSRLTGGLAGMAKG
                     RKVDVIQGDGQFLDSHHLEVSLTAGDVYEQATPTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVA
                     AGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELAAKASGRKITKANFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1906..2343)
                     /locus_tag="WJK66_000780"
     CDS             complement(1906..2343)
                     /locus_tag="WJK66_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578523.1"
                     /translation="MGNFLYRGISCQQDEQNNGQLKPKGNKAEVAIRYDGKFKYDGKA
                     THGPSVKNAVYAHQIETGLYDGCYISTTTDKEIAKKFATSSGIENGYIYVLNRDLFGQ
                     YSIFEYEVEHPENPNEKEVTIRAEDCGCIPEEVIIAKELIEIN"
     gene            complement(2437..4029)
                     /gene="aceF"
                     /locus_tag="WJK66_000781"
     CDS             complement(2437..4029)
                     /gene="aceF"
                     /locus_tag="WJK66_000781"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEZ7578524.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKVGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAEAQPAPAAAPVA
                     AGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVV
                     KAVFLKVGDKVSEGSAIIEVETAGSAAAAPAQAAAPAPAAAPAPTAVAAPAPSAPAAA
                     KIDEAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIVGDDIKAFVKSVMQGGAAK
                     PAAAGASLGGGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPHVTVHE
                     EADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDNLVLKN
                     YFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGACFTI
                     SSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDHRVIDG
                     AAGMRFTVFLAKLLKDFRRITL"
     gene            complement(4175..6838)
                     /gene="aceE"
                     /locus_tag="WJK66_000782"
     CDS             complement(4175..6838)
                     /gene="aceE"
                     /locus_tag="WJK66_000782"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEZ7578525.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKTLVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRVGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAIEAERFNRLNPLETAKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRSFFEVDRYNVAVAALSA
                     LADQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7532..9244
                     /locus_tag="WJK66_000783"
     CDS             7532..9244
                     /locus_tag="WJK66_000783"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEZ7578526.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDAYCRIFTRLGLEFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANVELAPTLPLKGERTAAQAELVKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGEIVLLLLRGDHEFNDIKAEKLAGVKSPLTMASPAAIVEQFGANGG
                     SLGPVGFAGKVYADFATEKGADWVIGANEDGYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMASFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRDNEAQAIAIGEIVARVTA
                     SLNA"
     gene            9307..9963
                     /locus_tag="WJK66_000784"
     CDS             9307..9963
                     /locus_tag="WJK66_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEZ7578527.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGTGEGKDILGCVAGYGVGLDLTA
                     RDIQCRLKEKGLPWLKAKGFRHSACVSDFAAAGRIGNPENVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(9973..10428)
                     /gene="ruvX"
                     /locus_tag="WJK66_000785"
     CDS             complement(9973..10428)
                     /gene="ruvX"
                     /locus_tag="WJK66_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEZ7578528.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLATVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(10421..10969)
                     /locus_tag="WJK66_000786"
     CDS             complement(10421..10969)
                     /locus_tag="WJK66_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEZ7578529.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPFRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKILISIGYSSWGKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(10971..11531)
                     /locus_tag="WJK66_000787"
     CDS             complement(10971..11531)
                     /locus_tag="WJK66_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEZ7578530.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNILGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENPDKR"
     gene            11859..13664
                     /locus_tag="WJK66_000788"
     CDS             11859..13664
                     /locus_tag="WJK66_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ7578531.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSARKGREGD
                     KKSKAEHKKAHGKPVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAA
                     TKAQISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQ
                     KALAVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLE
                     QKGNEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRA
                     EMSNLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGV
                     FYSTAPATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAA
                     GSKIGSSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13784..15268
                     /locus_tag="WJK66_000789"
     CDS             13784..15268
                     /locus_tag="WJK66_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEZ7578532.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDRMVLKAVPEDYVYGMGGDSLAGIPAELKTIPMTVLVNSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
BASE COUNT         3713 a         3768 c         4226 g         3656 t
ORIGIN      
        1 ccggaggaaa tattttaatc cgctgtaaaa tagaaaatgc cgtctgaaca tttcagacgg
       61 catttgctca aataaacctt atcggctgtt tattcagtcg gatttatttt ttcttttgcg
      121 gaggaaggtc ggtacaagta cccaatgcca cttccgccgc cataccgatg gattcgccca
      181 aggtcgggtg cgggtggatg gttttgccga tgtctgccgc gtcgcagccc atttcgatgg
      241 caaggcagac ttcgccgatc atgtcgccac cgttcggacc gacaatgccg ccgccgatga
      301 tgcggccggt ttcggcatcg aaaatcagct tggtaaagcc gttgtcgcaa ccgttggcaa
      361 tcgcacggcc ggaagccgcc cacgggaagt tggctttggt gattttgcgg ccggatgctt
      421 tggcggccaa ctcggtttcg cccacccacg ccacttcggg ggaagtgtag gcaacgccag
      481 gaatcacgcg cgcgtcgaag taggctttgt ggccggcgca gttttcagca gcaacgtggc
      541 cttcgtgaac ggctttgtgt gccaacatcg gttgaccgac gatgtcgccg atggcgtaga
      601 tgtgcggcac attggtacgc atttgtttgt ccacttcgat gaagccgcga tcggtaacgg
      661 caacgcctgc tttttccgcg ctgatgagtt tgccgttggg cgcgcggccg gcggcaacca
      721 atacggcatc gtagcgttgc ggctctttag gcgcgtttgc gccttcaaag gtaacgtaaa
      781 cgccgtcttc tttcggctcg actgcaacgg ttttggtgtt gaccataatg ttgtcaaaac
      841 ggtattcgtt ttgtttttgc catactttta ccaaatcgcg gtctgcgcct tgcatcaggc
      901 cgtccatcat ttcaaccaca tccaaacgcg aacccagcgt gctgtaaacc gtacccatct
      961 cgaggccgat aatgccgccg ccgataatca gcagtttgcc cggtacttct ttcagagcca
     1021 atgcgccgct ggaatcgatg atgcgcggat cttcagggat aaacggcagt ttggttacgc
     1081 ggctgcctgc tgcaatgata cagtttttga aggcaacgat ttttttctcg ccggtaggcg
     1141 ttgcttgttc gtacacgtcg ccggcagtca gcgatacctc caagtggtgc gaatctaaga
     1201 attggccgtc gccttggata acgtccactt tgcggccttt cgccatacct gccaaaccgc
     1261 cggtgaggcg ggaaaccacg ccgtttttgt aggcgcgcag catatcgatg tcgagttcag
     1321 gctcggggta tttgatgccg tttgcagcca agtggcgcac ttcgtcgata acggcggcat
     1381 tgtgcaacag ggctttggaa gggatacagc cgacgttcag gcaaacgccg cccaaggttt
     1441 tataacgttc gacgatggcg actttcaagc cttcatcggc agcggcaaat gcagcggagt
     1501 aaccgccggg gccgccgccc aatacgacca cgtcgtactc ggcatcggca gaaccgccga
     1561 attgcgcggc ttgcggggca ggagcagcag ctttaggggc ttcttgcgcc ggggcggcag
     1621 ctgcttcggc tttaggggcg gcagccgcac cttcggcttc gacgacgaca atcaaaccgc
     1681 cttcggagat tttgtcgccg actttgactt tcacttcttt gaccacgccc gcaacttcgg
     1741 caggcacgtc catcgtcgct ttgtcggttt ccaaggtaat cagggtgtcg tcaacggcga
     1801 tggtgtcgcc cacgttcact tcaactgcga taatatctac attttcgtgt ccgccaatgt
     1861 cgggcacttt caattcaact aaagccatta tattgacctt tcaacttagt taatttctat
     1921 caactcttta gcaataatca cttcttcagg aatacagcca caatcttcag ctctgattgt
     1981 tacttccttc tcatttgggt tttctggatg ttcaacctca tattcaaaaa tagaatattg
     2041 accaaacaaa tccctattta aaacatatat atagccattt tcgatgccgg aacttgttgc
     2101 aaatttcttg gcaatttcct tgtctgttgt cgtagatata taacatccgt catatagacc
     2161 tgtttcaatt tgatgggcgt aaactgcatt cttcacactt ggaccatgtg tagctttacc
     2221 atcatattta aacttaccat cataacgaat tgcaacttca gctttattac ctttaggttt
     2281 taactgtcca ttattttgct catcttgttg gcaactaatg cctctatata agaaatttcc
     2341 cataagttat taatttaaag gttgaattta acaccattac tcatcaatct caagcaatcc
     2401 aaacattatc agactgcttg agagggatgt tttattttat aaggtaatgc ggcggaagtc
     2461 tttcaacagc ttcgccaaga atacggtaaa gcgcataccg gccgcaccgt cgatgacgcg
     2521 gtggtcgaag gacaggctca atgggcacat caggcgcggg gcaaattctt tgccgttcca
     2581 aaccggtttg atttgggatt tgcacacgcc caagatggcg acttcgggag cgttcacaat
     2641 cggcgtgaaa cctgtgccgc cgatgccgcc caagctggaa atggtaaagc acgcgccttg
     2701 catttcttgc ggtttgagct tgccttcgcg ggctttttta gacaattcgg tcaattcttg
     2761 gctaatttgt ttcaagcctt tttgatctac gtctttgatg acgggaacaa ccaagccgtt
     2821 cggcgtatcg gctgcgaaac cgatgttgaa gtagtttttc agcaccaagt tgtcgccgtc
     2881 cagagaagcg ttgaattcgg ggaaggcttt cagcgcggaa acggaggctt tgatgatgaa
     2941 cgccaacggt gacagtttca cgccttcgcg ttcccattct ttgttcagct gtttgcggaa
     3001 ttcttccagc tcagtcatat ccgcttcttc gtgtacggta acgtggggaa tcacaaccca
     3061 gttgcgggac aggttttgac cggaaatttt cttaatgcgg gacaattctt taacttcgac
     3121 attgccgaat ttggagaagt ccactttagg ccacggcagc aagtccagac cgccgcccaa
     3181 agatgcgccg gctgcggcag gttttgccgc accgccctgc attacggatt tcacaaaggc
     3241 tttgatgtcg tcgcccacga tgcggccttt caagccggta cctttgactt ggcccaaatc
     3301 cacgcccaat tcgcgcgcca gtttgcgtgc ggaaggaccg gcgtgtgctt tggcgaaagc
     3361 ggcctcgtcg attttggcag cggcaggtgc agaaggtgca ggcgcggcga cagcagtcgg
     3421 agccggggcg gcagccggtg cgggtgcggc ggcttgagca ggggctgctg cggcagagcc
     3481 ggcggtttct acttcgataa tggcagagcc ttcggacact ttgtcgccga cttttaagaa
     3541 tacggcttta acgacaccgg cagcggtaca aggtacgtcc attgtcgctt tatcggtttc
     3601 caaagtaatc agcgtgtcgt cttcggcaac ggtgtcgccc actttgattt caacggcgat
     3661 tacatccaca tcggtatggc cgccgatatc gggaacggct acttgaacgg ttgcaccgcc
     3721 tgcggcaacg ggtgcggcag caggtgcagg ttgtgcttcg gcagcagcag gagcggcttc
     3781 ggcagcggcg gcaccggttt caacggtcag aataacgccg ccttcagaga ttttatcgcc
     3841 cactttaact ttaacttctt taacaacacc ggctgcatca gcaggtacat ccatagtggc
     3901 tttgtcggtt tccaaagtaa tcagggtgtc gtcaacggca atagtgtcgc ctactttgac
     3961 ttctacggcg atgatgtcga cgttttcatg accgccgata tcagggactt tgatttctac
     4021 gatactcatt tgagttcctt taatattttc agtttgatgc cgtctgaaaa tttgtttcac
     4081 acggcatcgg tacagtttgt tcaaaccatt caagcattcg gttttcagac ggcctgatgt
     4141 ctgaatgaag caaacaggcc gtctgaaaca tcaattagcg tttccagcta ggagctgaat
     4201 cggctttgat accatatttt tcaatggctt gttgaacggt ttctttgctg actttgcctt
     4261 gatcggccaa tgcgctcaat gcagcaacgg caacgttgta gcggtcaact tcgaagaagc
     4321 tacgcaggtt ggcacggctg tcggagcggc cgaagccgtc agtacccaag acgtggtagt
     4381 cgttagggat gtaggcacgg atgcggtcag catagctacg gatatagtcg gtagcggcaa
     4441 tcaccgggcc gtcatgacct tgcagttgag aagtaacaaa cggtactttt gcagtttcca
     4501 aaggattcag gcggttgaaa cgttctgctt cgatagcatc gcgatgcaac aggttgaaag
     4561 atgggcaaga ccaaatgtct gcttccacgc cgaagtcggc tttcagcagc tcggcaccgg
     4621 caatcacttc ttgcaaaatc gtaccggaac ccatcagttg gactttcttg tcgcctttgc
     4681 cgccggcttt cagcaggtac atacctttca agatgtcttg ttccgcacct tcgggcatat
     4741 ccggatgggt gtagttctcg ttcatcaggg tgatgtagta gaacacgtct tcattattgg
     4801 catacatacg gcgcagaccg tcttgtacga tgacggcgac ttcgtattgg aaagtcgggt
     4861 cataagatac gcagttcgga atcaggtcgg cctggatgtg gctgtggccg tcttcgtgtt
     4921 gcaggccttc gccgttcagc gtcgtacggc cggcagtacc gcccagcagg aagccgcgcg
     4981 cgtgcatatc accggctgcc catgccaagt cgccgacacg ttggaaaccg aacatagaat
     5041 agtaaatgta gaacggaatc atcgcgaagt tgctgttggc gtagctggtt gcagccgcaa
     5101 tccagtcggc catcgcgccc ggttcgttaa taccttcttg caagatttga ccgtcaacgg
     5161 attctttata gaacatcagt tggtctttgt cttgaggggt atattgctga cctttcggat
     5221 tccaaatacc gtattggcgg aacatacctt ccatgccgaa agtacggctt tcgtcgggaa
     5281 cgataggtac gacgcgtttg ccgatttttt tgtctttcag caaagtggac aggatgcgga
     5341 cgaatgccat cgtggtcgag aactcgcgtt caccgctgga tttgagttgt gcgtcgaatg
     5401 ctgacagctc gggcacttcc aatacttcct gcgtcggttt gcgttgcggc aggtagccac
     5461 ccaaagcatc gcggcgtgcg tgcaggtatt tgtattcttc cgtatcgggg gcaaaagtca
     5521 ggtaaggcag atcgccgctt tcgatttgtt cgtcggtaac cggaatgtca aagcggtcgc
     5581 ggaattgttt cagggacgct ttgtccattt ttttggcttg gtgggcaacg ttctgacctt
     5641 cgccggatgc gcccataccg taacctttaa tggttttcgc caagatgacg gtaggtttgc
     5701 cgtcggcatg gttcgctgcg cggtcgtagg cgttgtacac tttttgcggg tcgtggccgc
     5761 cgcggttcaa tgcccagagt tgctcatcgg tcatatcggc aaccaatgtt ttcagttcgg
     5821 gcgtattgaa gaagtgttcg cgcacatacg cgccgtcttt ggatttgtaa gtttggtagt
     5881 cgccgtccaa acattcttcc atacgttggc gcaggatacc gtctttgtct ttcgccaaga
     5941 ggcggtccca gcggcggccc caaatgactt tgacgacatt ccagccggcg ccggcaaagt
     6001 tgccttccaa ttcttggatg attttgccgt tgccgcgtac cggaccgtcc aagcgttgca
     6061 ggttgcagtt gatgacgaaa atcaggttgt ccaagccttc gcgtgcagcc agtgcgattg
     6121 caccttgaga ttcgggttcg tccatttcgc cgtcgccgca gaaacaccat actttacggc
     6181 ctttggtttt tgccaaacca cgcgattcca agtatttcag gaaacgcgcc tgataaatcg
     6241 ccatgatggg ccccaagccc atggatacgg tcgggaactg ccaaaagtcg ggcaagaggt
     6301 gggggtgcgg ataggaaggc agaccgtgtc cgtccacttc ttggcggaag ttgttcagct
     6361 ggtcttcggt cagacggccc tcgacgaatg cgcgtgcata gatgcccggg gcgacgtgac
     6421 cttggaagaa gaccaaatcg ccttcttcgc cttcgccttt ggctttccaa aagtggttga
     6481 aaccgacttc gtacatggtg gcggcagatt ggaaagatgc gatgtgccca cccagttcca
     6541 aatctttctt gccggcgcgc aatacgatgg cggcggcgtt ccagcgcacg aatgcgcgga
     6601 tgcggtgttc gatgttttgg tcgcccggaa tgcctttttc gttttcaacc gaaacggtat
     6661 tcaaatacgg ggtggtcgtg ccgtgtggca tacgtacgcc cttgtcgcgg cagtatttga
     6721 ccaggttttc caagaggtat tgcgcgcgtt cgccgccttc atattcgagg acggagctta
     6781 acgcgtccag ccactcttgg gtttcgatag ggtcaacatc gtgtaattgg gtggacatag
     6841 tatctatcct ttatgttgag tgtttaatcg gcagggaggg tgtagccaac ccctttattt
     6901 tcgtttgtga aaaataataa gcaaaaacga aaatttctaa atcaaaccga aaaaaataat
     6961 ttaagtttaa tgttttcaaa aaattataaa agttttggaa acaagggaaa acttgaaaaa
     7021 aatgttcgat ctgctccaca cgaaatccta tcagcaatcg ggtctgctgg caaataacca
     7081 tgcctgtaaa agcaggtttt atactgaagt tggcgtttgg taaggtcaag tctttttgat
     7141 ttcgaaatat tctgtttgat tttggcgata ttttttcgga attgtggtat atcgaatcat
     7201 tatgtctctg tcggcaattt tcctattgta gaaaatctat acacaagctt tgtggataac
     7261 cgtgttgata agccacggct tgtgttaaaa aagtcttacg caacaaatgt acgcttattt
     7321 tgattaaaaa ataggcacat ttaaatctat aaaaatcatg tgcttatgtt caatatgatg
     7381 tttctggtca tgtttttgcc gtctgttatg ggatttgtcc cgcgcttggg gcagggggtg
     7441 aattttggag gttggtgcaa aacctgcttt tttcgcgtta caatctgcac ccgatttact
     7501 tttgaatttg cctgtttgga aaaatacttt tatgaaagcc agccaattct ttatctctac
     7561 tttaaaagaa gcccctgccg aagccgcgct tgccagccac aagctgatga ttcgcgccgg
     7621 cctgattaaa gccaacgcgt ccggccttta tacttggatg ccgatggggc tgcgcgtgtt
     7681 acgcaaagtc gaaaacgtcg tgcgcgagga aatggcgcgc gcgggcagcg tggagctgct
     7741 gatgccggtg gtgcagcctg ccgagctgtg gcaggaatcc ggccgctggg aattttacgg
     7801 taaagaactg ctgcgcctga aagaccgcca cgaccgcgat ttctgcatgg gaccgacctg
     7861 cgaggaagtc atcgccgaca tcgtgcgtaa agagatcaac agctacaaac aactgccgaa
     7921 aaacttttac cacatccaaa ccaaattccg tgacgaagtg cgcccgcgct tcggtgtgat
     7981 gcgtgcgcgc gagtttgtca tgaaagatgc ttattccttc catgccgact acgcctcgct
     8041 tcagacgacc tatgatgcca tgtatgacgc ttactgccgt atcttcaccc gtctgggctt
     8101 ggaattccgc cccgttgccg cagataccgg cagcatcggc ggtaccggct ctcacgagtt
     8161 tcaagtgttg gcggaaagcg gcgaagatgt gattgcatac agcgacactt ccgattacgc
     8221 cgccaatgtc gagctggcac cgaccttgcc gcttaaaggc gaacgcaccg ctgctcaggc
     8281 agaactcgtc aaagtacaca cgccaaacgt caaaaccatt gagtctttgg ttgaattcct
     8341 gaatattccg gttgaacaaa ccctcaaatc catcgtggtt gaaggcgaaa atgaaggcga
     8401 aatcgtttta ttgctgttgc gcggcgacca tgagttcaac gacatcaaag ccgaaaaact
     8461 cgcaggtgtg aaatcgccat tgacgatggc aagccctgcc gcgattgtcg aacagttcgg
     8521 cgcaaacggc ggctcgctgg gtccggtcgg ctttgcaggt aaagtttatg ccgatttcgc
     8581 cactgaaaaa ggcgcggact gggtcatcgg tgcgaacgaa gacggctacc actataccgg
     8641 cttcaacttc gggcgcgatg ccgccgagcc tgaattcgtc gatttgcgta atgtggtcga
     8701 aggcgacgaa agccccgacg ggcaaggccg tctgaaactg gcgcgcggca tcgaagtcgg
     8761 acacgtcttc cagttgcgcg acaaatacac ccaagccatg aacgtgagct tcctcgacaa
     8821 caacggcaaa tcgcaaatca tggaaatggg ctgctacggt atcggcatca cccgcgtcgt
     8881 tgccgccgcc atcgagcaga acaacgacga aaaaggcatc atctggacga aggcgatggc
     8941 gtcgtttgaa gtcgttatcg tgccgatgaa ctacaaaaaa tcagacaccg tacgcgaagc
     9001 cgccgacaaa atctatgccg aattgctggc ggcaggcgcg gatgtgctgc tggatgaccg
     9061 cgacgaacgc gcaggcgtat tgctgaacga ttccgagctg ttgggtattc cgcaccgcat
     9121 cgtcatcggc gaccgcgcct tgaaagaagg caatgtcgaa tacgccgaac gccgcgacaa
     9181 cgaagcgcag gcaattgcaa tcggagaaat tgttgcacgt gtaacagctt cattaaatgc
     9241 gtaagcttgg tcgaatgccg tctgaaaata atttcagacg gcatttttac ttagggggaa
     9301 agtgaagtgg cttcagtatt tttagaagga gaggcagtcg aggtcggcaa tattttctgt
     9361 atcggcagga actatgccgc acatatcgaa gagttaaaaa acgaaatacc ttccgaacct
     9421 gtggtgttta tgaagccgtc aggcagcatt ttgaacagcg gcggtacaat cttgctgccc
     9481 gagtttagta gggatgtgca gtttgagtgc gaactcgtgc tgctggtcgg taaggattca
     9541 gacggcacgg gcgagggtaa agatattttg ggatgtgtcg caggatatgg tgtagggctg
     9601 gaccttacgg cacgggatat ccaatgccgt ctgaaggaaa aagggctgcc ctggctgaag
     9661 gcaaaagggt tcaggcattc cgcgtgcgtg tcggattttg cggcggcagg cagaatcggc
     9721 aatcccgaga atgtgttgtt ttcattaaaa caaaacggcg tattgaaaca gcgcggggat
     9781 accggtttga tgatttaccc gattcgggaa attttacaca aactggcggc agattacggt
     9841 ttggggaaag gggatttggt gtttaccgga acgccctccg gcgtgggcgc aatcggtgcg
     9901 ggggacaatt tggcattgga gctggacggc ttggtgcgcg cctctttcac ggtagggtgt
     9961 taaccgcgcc gcttaacctt cacgcccgtt gaaacattcc gccggaccgc cctcgaaaaa
    10021 accgtgcaag atggcttgcg ccgccacttg gtcgagcacc gatttgcgtt ttttgccgaa
    10081 gacctgtgct tccgaaagca ggctttcggc atagacggac gacagccgtt cgtcaaccca
    10141 atagacgggg agattgaacc tgccgttcag cctgcgtccg aacttgcgcg acaggtgcgt
    10201 catttcatgt ttcgtgccgt cggtatgcac gggcagtccg acgacaaaat aacgcggctg
    10261 ccattcttga accagcttgg cgattgccgc gaacttttca tcgttgctgc cgccggtaac
    10321 ggtggccagc ggatgggaca gccccaattc cgcatcgccc tgcgccacgc cgatgcgcgc
    10381 ttcgccgaag tcgaatgcca gtgccgttcc ttttggaatt ttatgcatgg ccggctcccg
    10441 aaaacagggc gagcgggtcg atgccgagtt tggcgaatgc ggcggcgtaa cggtgttcgt
    10501 aggggatgtc gaacaggata tgttcgtcgg cgggaacggt cagccacgca ttgtcggcaa
    10561 gttcgcgttc gagctggcct tttccccagc ttgaatagcc gatactgatt aatattttgt
    10621 caaccgcgcc ttcgcgtgaa atattttcaa tcacgtctcg ggaagaagtc agcgcgatat
    10681 tgtccgaaac gccgatactg ctttgccagt tgccgatcgg ggtatgcacg acataacctc
    10741 gctcgacttg caccggtccg cccatcatca cgctgtcgtg ctgcattcgg aaagggatgt
    10801 ttttgccggt ggcggaaaaa atcatgtcca tcgtaatcgg agagggtttg ttgatggcga
    10861 tgccgagtgc gccgtcttca tcgtgtttgc agatatagac gaccgattgt gaaaaaaacg
    10921 cgtcttccat atcgggcatg gcgacgagaa agtggttgga gaggttcata tcagcgtttg
    10981 tcgggatttt ctatcgggtt ggaaatcatg caggcgccgg caagcaggtt gccggaagta
    11041 tcggcttgca ccccatcggt ttgcggtatg ccgccgccga aacaggaaac cgcgcttaag
    11101 gaatttttcg gcgagccttg gatgatttta tcgctgtaca ccaaataggc gaaggttttg
    11161 cgtttggggt cgtaataacg gacaatctgc cggctcttga acgcgaagct cgcaccgtgt
    11221 ttgaaaactt ctttcggttt gcgcacggcg gtttcgtcaa aagaaatcga agatgccgtc
    11281 tgaacgcacg aaaccgatgc gtcggacgcg tcctcttcca aattgaccat ttccttcaag
    11341 ccgccttttt ttgcatacga aatataacag gcaacccctt gaacgtcggg atcgtcgaat
    11401 ccttccactt cgatacggtc gtttttgccc agtatgttga aaacggtact tgcccgtccg
    11461 attttatcgg tttcgccgct gccgcaggca gtcagcagga cggcggcaga cagcagtagc
    11521 agtctgttca tcaaaatttc cttatcggtt gaatccccgt tttccgtgaa ggcggcaggg
    11581 cttgctttgt ttggaggggc gcacgttccc aaaatcgggc aacatacggt atgcggctta
    11641 atgtgctacc ctgtgtgtcg gaacacaata tccgttgcgg cattgaaaat atgacgcaca
    11701 atataaggct gtttgagccg attgcaagta tccgcgcaaa gaaggcgcaa gattgggcaa
    11761 acggcatttt cagatggcat aactgacagt ataatccaaa cgtccggctg ctttcgtgca
    11821 gccttcagta ttatccaact ttttccgaag gtcgaaccat gcgctacaaa ccccttctgc
    11881 ttgccctgat gctcgttttt tccacgcccg ccgttgccgc ccacgacgcg gcacacaacc
    11941 gttccgccga agtgaaaaaa cagacgaaga acaaaaaaga acagcccgaa gcggcggaag
    12001 gcaaaaaaga aaaaggcaaa aatggcgcag tgaaagataa aaaaacaggc ggcaaagagg
    12061 cggcaaaaga gggcaaagag tccaaaaaaa ccgccaaaaa ccgcaaagaa gcagagaagg
    12121 aggcgacatc caggcagtct gcgcgcaaag gacgcgaagg ggataagaaa tcgaaggcgg
    12181 aacacaaaaa ggcacatggc aagcccgtgt ccggatccaa agaaaaaaac gcaaaaacac
    12241 agcctgaaaa caagcaaggc aaaaaagagg caaaaggaca gggcaatccg cgcaagggcg
    12301 gcaaggcgga aaaagacact gtttctgcaa ataaaaaagt ccgttccgac aagaacggca
    12361 aagcagtgaa acaggataaa aaatacaggg aagagaaaaa tgccaaaacc gattccgacg
    12421 aattgaaagc cgccgttgcc gctgccacca atgatgtcga aaacaaaaaa gccttgctca
    12481 aacaaagcga aggaatgctg cttcatgtca gcaattccct caagcagctt caggaagagc
    12541 gtatccgcca agaacgtatc cgccaagagc gtatccgtca ggcgcgcggc aaccttgctt
    12601 ccgtcaaccg caaacagcgc gaggcttggg acaagttcca aaaactcaat accgagctga
    12661 accgtttgaa aacggaagtc gccgctacga aagcgcagat ttcccgtttc gtatcgggga
    12721 actataaaaa cagccagccg aatgcggttg ccctgttcct gaaaaacgcc gaaccgggtc
    12781 agaaaaaccg ctttttgcgt tatacgcgtt atgtaaacgc ctccaatcgg gaagttgtca
    12841 aggatttgga aaaacagcag aaggctttgg cggtacaaga gcagaaaatc aacaatgagc
    12901 ttgcccgttt gaagaaaatt caggcaaacg tgcaatccct gctgaaaaaa cagggtgtaa
    12961 ccgatgcggc ggaacagacg gaaagccgca gacagaatgc caaaatcgcc aaagatgccc
    13021 gaaaactgct ggaacagaaa gggaacgagc agcagctgaa caagctcttg agcaatttgg
    13081 agaagaaaaa ggccgaacac cgcattcagg atgcggaagc aaaaagaaaa ttggctgaag
    13141 ccagactggc ggcagccgaa aaagccagaa aagaagcggc gcagcagaag gctgaagcac
    13201 gacgtgcgga aatgtccaac ctgaccgccg aagacaggaa catccaagcg ccttcggtta
    13261 tgggtatcgg cagtgccgac ggtttcagcc gcatgcaagg acgtttgaaa aaaccggttg
    13321 acggtgtgcc gaccggactt ttcgggcaga accggagcgg cggcgatgtt tggaaaggcg
    13381 tgttctattc cactgcaccg gcaacggttg aaagcattgc gccgggaacg gtaagctatg
    13441 cggacgagtt ggacggctac ggcaaagtgg tcgtggtcga tcacggcgag aactacatca
    13501 gcatctatgc cggtttgagc gaaatttccg tcggcaaggg ttatatggtc gcggcaggaa
    13561 gcaaaatcgg ctcgagcggg tcgctgccgg acggggaaga ggggctttac ctgcaaatac
    13621 gttatcaagg tcaggtattg aacccttcga gctggatacg ttgatcggat tgccgaaacg
    13681 gtaggcttta tgcaaatcgt gccgatatgg tgcgctgttc cgtgaagttt gccgtacctg
    13741 tattttccgg tttttggaaa tcaagagaga aagttaaaga agaatgtcga aacctgtttt
    13801 taagaaaatc gcactttata ctttgggtgc aatcagcggc gtggccgtca gtctggcggt
    13861 gcagggtttt gccgccgaga aggacaggcg ggataacgaa gtcctgccgg tgcaatccat
    13921 ccgcacaatg gcggaggttt acggtcaaat caaggcaaac tactatcagg acaaacccga
    13981 tgccgatttg tttgaaggtg cgatgaaggg tatggtggcc ggtttggatc cgcattccga
    14041 atatatggat aaaaaaggtt atgccgagat aaaggagtcc accagcggcg aatttggcgg
    14101 cttggggatg gaaatcgggc aggaagacgg atttgtcaaa gtggtttcgc cgattgagga
    14161 cacgcctgcg gaacgggcgg gggtgaaaag cggcgatttc attgtgaaaa tcgataatgt
    14221 ttcgacacgc ggcatgacgg tcagcgaagc ggtgaagaaa atgcggggca agccgggtac
    14281 gaagattact ttgacgctgt cgcgcaaaaa tgccgacaag ccgatagtcg tcaacctgac
    14341 ccgtgccatt attaaagtga aaagcgtccg ccatcacctg atcgaacccg attacggcta
    14401 tatccgcgtg tcgcagttcc aagagcggac ggtcgaaagc gtcaataccg ccgcaaaaga
    14461 gctggtaaag gaaaataaag gaaaaccgct caaggggctg gtgttggatt tgcgcgacga
    14521 ccccggcggg cttttgactg gcgcggtcgg cgtgtcggcg gcatttctgc cgtctgaagc
    14581 agtcgtcgtc agcaccaagg gacgcgacgg caaagaccgc atggtactga aagccgttcc
    14641 tgaagattat gtgtacggga tgggcggcga ttcgttggcg ggcattcctg ccgagttgaa
    14701 aaccataccg atgacggtat tggtcaattc cggttcggct tccgcgtcgg agattgtcgc
    14761 aggtgcattg caggatcata aacgcgcggt catcgtcggt acgcagagct tcggcaaagg
    14821 ttcggttcag actttgattc ctttgtccaa cggcagcgcg gtcaagctga caacggcact
    14881 gtattatacg ccgaacgacc gttctattca ggcgcagggg attgttcccg atgtcgaagt
    14941 aaaagataag gaacgcattt ttgaaagccg cgaggcggat ttggtcggac acatcggcaa
    15001 tcctttgggc ggcgaggatg tgaacagtga aacccttgcc gtgccgcttg aaaaagatgc
    15061 ggataagccc gctgtaaaag aaaaaggtaa aaagaaaaag gacgaggatt tgtcttcaag
    15121 gcggattccc aaccctgcca aagacgacca gttgcggaaa gctttggatt tagtcaagtc
    15181 gcccgagcag tggcagaagt ctttggggct ggcggcgaaa aagccggttt caaataaaga
    15241 taagaaagat aaaaaagata agaagtaggt tttgacgatg ccgtctgaaa ggatttcaga
    15301 cggcatatta tgtttgaaag gacaggttat gagcgatttt ttaccggaat ttgaaccgtg
    15361 cgg
//
LOCUS       DAWKOG010000033        14903 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.033, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000033 DAWKOG010000000
VERSION     DAWKOG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14903
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.033"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(87..3572)
                     /gene="smc"
                     /locus_tag="WJK66_000790"
     CDS             complement(87..3572)
                     /gene="smc"
                     /locus_tag="WJK66_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEZ7578533.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNDEVHALQ
                     TAEQSQQQAVHELGNKRGVLREQIARLEEQIRHQQNLHQRIERDKQAAQAQMQRIHQE
                     QQQIRVQLEENELYVEEKQTELAEWAMQVAEHEERLPELEETQTALNAAFQTQQDEAN
                     RIRRELALKQQQLAHTGQTIAGHEEHKGRLKQENQALNLPDEAETAAAQEAAALLQNR
                     QEHYEEQIIAAEEALHAAREAFQTASSRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDHAAAPQLWQHITAPAEWQHALSVILAERLHARAVPQGFVPPEPLPQGQAAWL
                     SDDLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQSDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELDNLAPELSADEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIATLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYRQQIARLEQQTLDWQERQQELALAYETEFQNDEQHIKLEELS
                     EAVQSLDEEYIVVQEKLAQIQEQGREQYARVQTLQTKLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNSSIGSLSQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAITILEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCNLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            3821..4543
                     /gene="lpxH"
                     /locus_tag="WJK66_000791"
     CDS             3821..4543
                     /gene="lpxH"
                     /locus_tag="WJK66_000791"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEZ7578534.1"
                     /translation="MKPAYFISDLHLSEKQPELTALLLRFLRSSAARQARAVYILGDL
                     FDFWVGDDEVSELNTSIAREIRKLSDKGVAVFFVRGNRDFLIGQDFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDKAYQRFRKIVHRKRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFGAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKY"
     gene            4812..6398
                     /locus_tag="WJK66_000792"
     CDS             4812..6398
                     /locus_tag="WJK66_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ7578535.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     DDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYAVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(6567..6770)
                     /locus_tag="WJK66_000793"
     CDS             complement(6567..6770)
                     /locus_tag="WJK66_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEZ7578536.1"
                     /translation="MDNLNLQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            6886..7383
                     /locus_tag="WJK66_000794"
     CDS             6886..7383
                     /locus_tag="WJK66_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HEZ7578537.1"
                     /translation="MTALTLPEDIRQQEPSALLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGKTIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHIQSNWETFA
                     HIGQA"
     gene            7465..8658
                     /locus_tag="WJK66_000795"
     CDS             7465..8658
                     /locus_tag="WJK66_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ7578538.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PLVKAVKEAEKRLLESETTKNYLTIDGVADYNAQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVGLLKAKG
                     ASQNFDFIIKQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDNNIDYLCES
                     IVKVL"
     gene            complement(9062..9997)
                     /gene="hemC"
                     /locus_tag="WJK66_000796"
     CDS             complement(9062..9997)
                     /gene="hemC"
                     /locus_tag="WJK66_000796"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEZ7578539.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYTRLEEMPEGAVVGTSSLRREAQLRARYPHLLIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLKLDGRIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHGVTNACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVVLRADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            10116..10631
                     /gene="ybeY"
                     /locus_tag="WJK66_000797"
     CDS             10116..10631
                     /gene="ybeY"
                     /locus_tag="WJK66_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEZ7578540.1"
                     /translation="MKRTKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KNEYRRADISLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPEQHFAHLTIHGTLHLMGYDHIEDDEAEIMEAEEIRLMRAA
                     GFPNPYQEDGH"
     gene            10633..11457
                     /locus_tag="WJK66_000798"
     CDS             10633..11457
                     /locus_tag="WJK66_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEZ7578541.1"
                     /translation="MDGAQPKTNFFERLIARLAREPDSAEDVLNLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSILRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGEIEDEFDEDDSADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(11586..12965)
                     /gene="nhaC"
                     /locus_tag="WJK66_000799"
     CDS             complement(11586..12965)
                     /gene="nhaC"
                     /locus_tag="WJK66_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEZ7578542.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFHADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPSVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVILALMRVNAVVAMLFTVMVAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDVVKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GVIPSLLEAIRTFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDKLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(13256..14479)
                     /locus_tag="WJK66_000800"
     CDS             complement(13256..14479)
                     /locus_tag="WJK66_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HEZ7578543.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTSVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
BASE COUNT         3527 a         4209 c         3828 g         3339 t
ORIGIN      
        1 tttaaattta attcactata ccgtctgaaa acctgtatcg caacacgtca ttaagaacgg
       61 caagattcaa ccgttctgca acccgttcaa acggcttccg ccatttccaa cgcttgtttg
      121 atgtccacgg cgacgacgcg cgatacgcct ttttcctgca tcgttacgcc gacgagctgc
      181 tctgccattt ccatcgtcag gcggttgtgg gagatgtaga ggaactgggt ttgcgccgac
      241 atttctttga ccaggttgca gaagcgcgag gtgttggcgt cgtccagcgg ggcatcgact
      301 tcgtccaaaa ggcagaacgg agcggggttg aggctgaaca gagcgaacac gaggctcatg
      361 gcggtgaggg ctttttcgcc gccggagagg aggtggatgg tgctgttttt cttgccgggc
      421 ggacgcgcca taatggacac accggcggtc agtaggtcgt cgcctatcat tttgagagtg
      481 gcttcgccgc cgccgaacag ggtcgggaag aaggtttgga ctttgccgtt gacggcgtcg
      541 aaggtttctt tgaaacgcgc tttggttttg tcgtcgattt gggcgatggc ttcttccaaa
      601 atggtgatgg cggcctgcac gtcttcgctt tggctgcggt agtagccgtc gcgttcgcgc
      661 gcttcttcga gttcttgcag ggcggcgagg ttgaccgcgc cgagggcttc gatttgttgc
      721 gagaggctgc cgatgctgct gttcaatact ttcggcgatt ctttcgccaa cgcttcgagc
      781 gcatccaaat cggcggcgcg ttcggtcagg ttttgatggt agcgtttggc gttgatcagg
      841 gcttcctgtt gttgcaacag ggcggtttgg gtggcggcct gaagctgtgg cagcttggtt
      901 tgcagggttt gtacgcgggc gtattgttcc ctaccctgct cctgaatctg cgcgagtttc
      961 tcctgtacga caatatattc ttcgtccaaa ctctgtacgg cttcgctcaa ctcttcgagc
     1021 ttgatgtgct gctcgtcgtt ttggaactcg gtttcatagg caagggcaag ctcttgctgg
     1081 cgttcctgcc agtctagggt ttgctgttcg agtcgggcga tttgctgccg gtagttttgt
     1141 ttttgctggt tgagcttgtg gacggcgact tcggccagcc cgtattggcg gttggcttcc
     1201 aacagggcaa gctgcgcctg tttcagacgg ccttgctgct cttggcggct gtgtgcggtg
     1261 gtctgctgtt ggtgttcgag ttcggcggcg gcttcctgca aagtagcgat gtcgtctgaa
     1321 agcccgtcgg acgtgtgttg caacacggtc tgctcttccg ccaactgcgc cagttcgcgc
     1381 tcgatgtgtt cgcggcggat ttgcccttgg ttggtgcgcg ccaagagttc ggcggcgcgt
     1441 tgctgcgctt ggctgtattg gcgcgtgtgc tgctgttgct gctgcatcag gtttttatgt
     1501 tgcacttcgg acgagcgcac ggcggcttct gcctgtttga acgcggcttc gtcagcggag
     1561 agttcggggg ctaggttgtc cagttcggat gcgatgccgt cgaggcgcgc tttttgggca
     1621 atcaggcttt cctgcgcggg cttggcatag agcaggacgc tgaccttatc gacctgatga
     1681 ccttcgggcg tgagccagat ttgatgtgcg cccaaatcgc tttgatgcgc gagggcatag
     1741 ctcaaatcgg gcgcgcacaa tacgccgtcg agccagtggt gcaatgccgt ctgaaacggc
     1801 ggctgcgctt ggatttggtt cagcaatgcc tgtacgggca gggatttttt gatgccgcct
     1861 gagaggtcgt ctgaaagcca tgccgcctgc ccctgcggca aaggctcggg cggcacgaaa
     1921 ccttgcggca cggcgcgggc gtgcaggcgt tcggcaagaa tgacggacaa ggcgtgctgc
     1981 cactcggcgg gcgcggtgat gtgttgccac agttgcggcg cggcggcgtg gtcggttgcc
     2041 tgccagaaat cggcggcttc ctgctgttgc gacaggattt gcgacaacgc ctgctgctgc
     2101 gcctgcaagg taatgtgttg ctgcttcagg ctttggaagc ggctcgatgc cgtctgaaac
     2161 gcctcgcggg cggcgtgtag agcttcttcg gcggcgatga tttgttcttc gtaatgctct
     2221 tgccggtttt gcaacaaggc ggcggcttcc tgcgcggcgg cggtttcggc ttcgtcgggc
     2281 aggttcaggg cttggttttc ctgtttcaga cggcctttgt gctcttcgtg cccggcaatc
     2341 gtttgtccgg tatgggcaag ctgctgctgt ttcaacgcca gttcgcggcg gatgcggttt
     2401 gcctcgtcct gctgggtttg gaaggcggca ttgagcgcgg tttgggtttc ttccaattcg
     2461 ggcagacgct cttcgtgttc ggcaacctgc atcgcccact ccgccagctc ggtttgcttt
     2521 tcttcgacgt ataactcgtt ttcttccagc tgcacgcgga tttgctgctg ctcttgatga
     2581 atacgctgca tctgcgcctg cgccgcctgc ttgtcgcgtt cgatgcgttg gtgcaggttt
     2641 tgctgatggc ggatttgttc ttccaaacgg gcaatctgct cgcgcaacac accgcgtttg
     2701 ttgcccagtt cgtgtaccgc ctgctgctgc gactgttcgg cggtctgcaa ggcgtgtact
     2761 tcgtcgttta acgcctgaac ctgcgcggcg gtttcgtcct gctgcgcctg caaagactga
     2821 tgctgcgcgg tcgccttgtc ggcggcggca agcgattgct gccattgggc gtaatcgagc
     2881 aaatcctgtt gctggttcag ctgcgcggtc agggatttgt agcgttcggc ggtttccgcc
     2941 tgtttctcca gcttttccac ctgacgcgct aactcgttct gcaaatcgcc caaacgctgc
     3001 aaatgctcgc gcgtgtcttt cagacggcct tccgtctcct tacggcgttc cttatatttg
     3061 gacacgcccg ccgcttcctc gatataggcg cgcaattcct ccggccgcgc ttcgatgatg
     3121 cgcgaaatca tcccctgctc gataacggca taaccgcgcg cgcccacgcc cgtacccaaa
     3181 aacaaatcgg taatgtcgcg gcggcgcacg gtctgattgt tgatgaaata agtcgattcg
     3241 ccctgacgcg tcagctgccg cttgatgctc acctcggcat actgccccca cgccccctgc
     3301 aaactgtggt cgctgttgtc aaataccagc tccaccgaag ccctcggcgc aggacggcgc
     3361 gtcgccgcac cgttaaaaat cacgtcctgc atactctcgc cgcgaagctg cttcgccgaa
     3421 gcctcaccca acacccagcg caccgcgtca atcacattcg acttgccgca accgttcggc
     3481 ccgataaccg cgacaagctg ccccggaaca tgaatcgtgg tcgggtcggt aaaagatttg
     3541 aagccggaga gtttgatgtg ggtcaggcgc ataatggtcg gaaaaaataa aaaagaagcg
     3601 tattttaacg gaaatcccgc cgcaccaccc catatcttgc cggcaaaacc ttaccgcatc
     3661 ccgcacccgc gatgccgtct gaagccttca gacggcattt ttaacgcgcc gaaccccggt
     3721 tccgccaatg cccgatgctt ggcgcggcat ttgccgtatt gccccgccgc cgggcgcgtt
     3781 ataatccgcc acgacatccg cccgatttgg aaacctgcct atgaaacccg cctatttcat
     3841 ttccgacctg catttgagcg aaaagcagcc cgaactgacc gcgctgttgc tgcgtttttt
     3901 acgttcttcc gccgccaggc aggcgcgggc ggtttacatt ttgggcgatt tgtttgattt
     3961 ttgggtgggc gatgacgaag tttccgagtt gaatacttcg attgcgcgtg aaatcaggaa
     4021 attgtccgac aaaggcgttg ccgtgttctt cgtcaggggc aaccgcgact tcctgatcgg
     4081 tcaggatttt tgccggcagg cgggcatgac gctgctgccg gattactcgg ttttggactt
     4141 gttcggctgc aaaaccctca tctgccacgg cgacacatta tgcaccgacg acaaggcata
     4201 ccaacgtttc cgcaaaatcg tgcatcggaa gcggctgcaa aaactgttcc tgatgctgcc
     4261 cctaaagtgg cgcacgcgcc ttgccgccaa aatcaggcgt gtcagcaaaa tggaaaaaca
     4321 ggtcaagccc gccgatatta tggatgtcaa tgccgccttt accgcgcggc aggtacgcgc
     4381 cttcggtgcg gaaaggctga tacacggaca cacccaccgc gagcatatcc atcacgaaaa
     4441 cggctttacc cgcatcgttt tgggcgactg gcataacgac tatgcttcaa tcctccgcgt
     4501 ggacggggac ggcgcggtat tcgtgccgct ggaaaaatac tgaaaatgcc gtctgaagcc
     4561 tttcgggcgg ctttttttac acgccccttt cccacactcc ctccgccgca tcatcaccat
     4621 ccttaacccg cattaatagc cattatcgat aaaatgcttg accgattaca taagattacg
     4681 taaagtgtgc aaacgcataa caattggtct taccaattta tcttttaacc aaaatatcgg
     4741 gtgtacggga tttgaacgtc tttccaaacc tcgacgcgcc ttatgatgca aatgcctaaa
     4801 aaggattcgt tatggcactt ttcctcagca tattccccat cgtcctgctg atttggctga
     4861 tggtgaaaaa aaacagtatg ccctcctacg tcgcgctgcc gattaccgca gtgctgattt
     4921 acgccatcaa acttttctac ttcgacgatg cgggcatgct gctcaacgcc accgccgctt
     4981 ccggcctcgt caaaacgctc acgccgatta ccgtgatttt cggcgcgatt atgttcaacc
     5041 gtatgatgga aaccacgggc tgcatcgatg tcatccgcaa atggctggcg accatcagcc
     5101 cgaaccccgt agcgcaactg atgattatcg gctgggcttt tgcctttatg attgaaggcg
     5161 catccggctt cggtacgcct gccgcgattg ccgcgccgat tctgatgagc ttgggcttca
     5221 acccgttgaa agtggcgatt ttcactttgg tgatgaactc cgtccccgtc tctttcggcg
     5281 cggtcggtac gccgacctgg ttcggtttcg caccgctgaa cctgagtgcc gaagacatcc
     5341 tcgccatcgg caggcagacc ggcgtaatgc acttcttcgc aggtttcgtc atccccgtca
     5401 tcggcttggg cttcatcgta ccttggtctg aaatccgcaa aaacttgggc ttcgtcgcca
     5461 ttgccgtctt ctcctgcacc attccttatg tcgcattggc gatggtcaac gaagaattcc
     5521 cgtcgctcgt cgccggcgcg atcggcctga tggtgtccgt attcgccgcc aaccaaggct
     5581 ggggcttgag caaagaccac gccaaagacc cgaatgccga aaaagtgccg ttcgcccaag
     5641 tcgccaaagc actcgcccct ttgggtatgc tgatcggcat gctggtggtt acgcgcatca
     5701 aacagctcgg catcaaaggc attttgacca gcaaagaaga atggttcagc ttccaactgc
     5761 cgtttgattt gtccaaaatc accgtcagcg actccctgac gattactttc ggcaatattt
     5821 tcggacaaga tgtcagcgcg tcttaccaaa cgctgtacgt cccggcttgg attccgtttg
     5881 tgctgaccgt ttggatttgc atcctgctgt ataaaaccaa attcaaagat gcctggacga
     5941 tttatgccgt aactttcaat caaaccaaaa aaccgctgct tgccctgatg ggcgcgctga
     6001 ttatggttca gctgatgctg gtcggcggcg acaattcgat ggtgaaaatc atcggtaagg
     6061 aatttgccgc aatggcgggc gaacactggg tttatttctc gccgtatctg ggcgcgatcg
     6121 gtgcgttctt ctccggctcc aacaccgtgt ccaacctgac cttcggaccg attcagcagc
     6181 aaatcgcctt ggataccggc ctgtccgtta ccctgattct ggcgttgcag tccgtcggcg
     6241 gcgcgatggg caatatggtg tgcctcaaca acatcatcgc cgtatgtacc gtattggacg
     6301 tgaaaaattc cgaaggtgcg attatcaaga aaaccgttat cccgatggcg atttacggcg
     6361 tgattgccgt cgtcgcggca atgattttct tcctctaggc gaaacgccac catccatagc
     6421 ggcagcacgc ggggaacgga aaccgccgtt ccccgtccgg gcggcgcgtc gggcagaatc
     6481 ccggtgccgg aacaagcccg ccccgccaaa caaatgccgt ctgaaaccgg aaaaggcttc
     6541 agacggcatt tttccgccgc ttgcgttcag gcgtaatgat gatgcggcat cttgccgtca
     6601 aggcggtagc gcgtgccgca atacgggcag gcaacgctgc ccgattctcc ttcgcacaaa
     6661 ggcaaaaaca ctctcggatg cccgttccac tgctcgttgt cgggtcccga gcaatacagc
     6721 ggcagatttt ccggcaacac ggaaatttcc tgcagattca gattgtccat ttgattttcc
     6781 tttgcgtggt tggcgtgcgc ccattttacg ccatcggcag gctaaaggat attttcggcg
     6841 caaagccgca atccgctata atccccactt ttcagacggc ataccatgac tgcgcttacc
     6901 cttccggaag acatccgcca acaagagcca tccgccctgc tctataccct cgtttccgcc
     6961 tacctcgaac acaccgccca aaccggcgac gaatccctct cctgcctgtc cgacgaccag
     7021 cacacgctga ccgcattctg ctacctcgac agccaagtcg aagaaggcgg cttcgtgcaa
     7081 ctcatcgcct ccggttacgg cgaatatatt ttccgcaacc cgcttgccga cagcctgcgc
     7141 cgctggaaaa tcaaagccgt gccgaaagtc ttggacaaag ccaaagccct ctacgaacaa
     7201 cacggcaaaa ccatcgaaac gctcgccgac ggaggcgcag acatcgaatc cctgcgcaaa
     7261 cagttccccg actttgaaga atgggacggc gcatactacg aagccgccga acaagacctg
     7321 cccctgcttg cagaacacat acagtcaaac tgggaaacct tcgcccatat cgggcaggcg
     7381 tgattgcgtc tgtttccagc ccgtgtaaaa cagcgtaaaa tcggcaaacc cgaatgacct
     7441 tccgtcccca tcaaggagca agctatgttc ttcaagcaca tcgaagccgc ccccgccgat
     7501 ccgattctcg gtttgggcga agcgttcaaa gccgaaaccc gccccgaaaa agtcaacctc
     7561 ggcatcggcg tttataaaga cgcatccggc gcgacacccc tcgtcaaagc cgtcaaagaa
     7621 gccgaaaaac gcctgttgga aagcgaaaca accaaaaact acctcaccat cgacggtgtt
     7681 gccgactaca acgcgcaaac ccaaatcctg ctgttcggca aagaccacga aatcatcgcc
     7741 agccgtcgcg ccaaaacagc gcaaagcctc ggcggtacgg gcgcattgcg tattgcggcc
     7801 gagtttgcca aacgccagtt gaacgcgcaa accatctgga tttccaatcc gacttggccc
     7861 aaccacaacg ccatcgccaa agcggtcggt atccaagaca aaccttatcg ttactatgat
     7921 gccgccaaac acggtttgga ttgggacggc atgattgagg acttgagcca agcgcaaaaa
     7981 ggcgacatcg tcctgctgca cggctgctgc cacaatccta ccggcatcga ccctacgccc
     8041 gaacaatggg aaactttggc aaaactttct gccgaaaaag gctggttgcc gctgtttgac
     8101 tttgcctacc aaggcttcgg caatggtttg gaagaagatg cctacggctt gcgcgtgttc
     8161 ttgaaacaca atacagaatt gctgattgcc agctcttatt ccaaaaactt cggtatgtac
     8221 aacgagcgcg tcggcgcgtt cactttggtg gccgaagatg aagaaacagc agcccgcgcc
     8281 cacagccaag tcaaaaccat catccgtacc ttgtattcca acccggcttc acacggtgcg
     8341 aacaccattg cgctggtgtt gaaaaatgat gatttgaaag cacaatggat tgccgaactc
     8401 gatgaaatgc gcggccgcat caaagccatg cgccaaaaat ttgtcgggtt gctcaaagcc
     8461 aaaggtgcaa gccaaaactt tgatttcatt atcaaacaaa acggtatgtt ctctttcagc
     8521 ggcttgactc ccgaacaagt cgaccgcctg aaaaacgagt ttgccattta tgccgtccgc
     8581 tccggccgca tcaacgtcgc cggcattacc gacaacaaca tcgattatct gtgcgaaagc
     8641 atcgtgaaag tactgtaaaa agcacagcca tatgaaaatg ccgtccgaac cttaaccagt
     8701 tcagacggca tctgtttttt gttaaaaaac aataaaccat aacaatttca aacaagctaa
     8761 aaacatctat aaaatcaacc aaatatgatg aactcaaata aaaaatacga atatcatcat
     8821 tctcaaattc aaaatttgaa cgccacagta ttatccgaag ccacaaaaac ctttccgccg
     8881 tcattcccgc gcaggcggga atccgggaat ccaatgctgc aagaattgat tagaaacaac
     8941 tgaaaccgag caaactggat tcccgcctgc gcgggaatga cgaaataagg cgagtgaaac
     9001 tgatgaaaca aattgattaa aaacaataac ttatattatt ttatttaaat tttcgataaa
     9061 atcaattttc cgtattcaat actgctccaa tcaattcccg cgcaccgtcg tccgccaatt
     9121 tcttagcgac agcgcgtccg agcgcgtcgg catattcggc aggggcttgc gcgtccgccc
     9181 gcaacacaac cgaaccgtcg gggtgtccga ccaagccgcg caaggtcagc aagccgtttt
     9241 cttccgtgca atatgcggcc aaaggcactt ggcagcttcc gcccaaagcg cgtgcgaggg
     9301 cgcgttcggc ggtaacgcag gcattggtaa caccgtggtt caagggtttc aaaacttcat
     9361 acaaatcttc gcggtgcgcg gcaatttcga tacccaatgc gccttgtccg gcggcaggca
     9421 ggctgtcgga ttccgacaaa atcatgcgga tgcgtccgtc caatttcaga cgctgcaaac
     9481 cggcggcagc caagataatt gcgtcgtatt cgccgttatc gagtttggac aaacgggttt
     9541 gcacattgcc gcgcaaaggt ttgataagca aatgcggata gcgcgcacgc aactgggctt
     9601 cgcggcgcag gctggatgtg ccgacaaccg cgccttcggg catttcttcc aaacgcgtgt
     9661 attggttgga cacaaacgcg tcaaacggat tggcgcgttc gccgatggcg gcaagcgcga
     9721 aaccttcagg caaatccatc ggcacgtcct taatcgaatg caccgccaaa tcggcgcgcc
     9781 cgtcatataa agcctgttcc aactctttga caaacaagcc tttaccgccg acttttgaca
     9841 aagttttgtc caaaatctga tcgccgcgcg tggtcatgcc caaaatctcg acttcgcaat
     9901 cgggatacag agccttcaga cggccttgga tatgctttgc ctgccacata gcaagcaggc
     9961 tttcgcggct ggcgatgacg agttttttcg ggttcatgtt caatgcgttc aaagaatgga
    10021 aacggcgtga agtctatcat attttgaacg tttcttcaga cgacaccgtc aggtctatgt
    10081 tatatttaca cccacaaaac acgaaacccg ccacaatgaa acgcaccaaa aaataccctt
    10141 tcttaacgtt gcagcggcaa cgtttccatt tgaactttga aaacgcctct tccgccgccg
    10201 gcatcccagc cgaacgcgat ttctaccgct gggcgtggtc tgccttgaaa aatgaatacc
    10261 gccgcgccga catcagcctg attcttctgg acgaagaaga agcccgagcc tacaaccgcg
    10321 actaccgcgg caaagattac gccaccaatg tattgagttt cgcgctcaac gaaggcgaaa
    10381 tcctgccctg ccaagtttcg gaaaaactgt acggcgattt gattatctgc ccgcaagtgg
    10441 ttttgaaaga agccgccgaa caaggcaaaa cacccgagca gcattttgcc cacctgacca
    10501 tacacggtac tttacacctg atgggctacg accacatcga agacgacgaa gccgaaataa
    10561 tggaagccga agaaatccgc ctgatgcggg cggcaggctt ccccaacccc taccaagagg
    10621 acggacatta aaatggacgg cgcacaaccg aaaacgaatt tttttgaacg cctgattgcc
    10681 cgactcgccc gcgaacccga ttccgccgaa gacgtattaa acctgcttcg gcaggcgcac
    10741 gagcaggaag tttttgatgc ggatacgctt ttaagattgg aaaaagtcct cgatttttcc
    10801 gatttggaag tgcgcgacgc gatgattacg cgcagccgta tgaacgtttt aaaagaaaac
    10861 gacagcatcg agcgcatcac cgcctacgtt atcgataccg cccattcgcg cttccccgtc
    10921 atcggcgaag acaaagacga agttttgggc attttgcacg ccaaagacct gctcaaatat
    10981 atgtttaacc ccgagcagtt ccacctcaaa tccattctcc gccccgccgt cttcgtcccc
    11041 gaaggcaaat cgctgaccgc ccttttaaaa gagttccgcg aacagcgcaa ccatatggcg
    11101 attgtcatcg acgaatacgg cggcacatcc ggcttggtca cctttgaaga catcatcgag
    11161 caaatcgtcg gcgaaatcga agacgagttt gacgaagacg atagcgccga caatatccat
    11221 gccgtttctt ccgaacgctg gcgcatccat gcagctaccg aaatcgaaga catcaacacc
    11281 ttcttcggca cggaatacag cagcgaagaa gccgacacca ttggcggcct ggtcattcaa
    11341 gagttgggac atctgcccgt gcgcggcgaa aaagtcctta tcggcggttt gcagttcacc
    11401 gtcgcacgcg ccgacaaccg ccgcctgcat acgctgatgg cgacccgcgt gaagtaagca
    11461 ccgccgtttc tgcacagttt aggatgacgg tacgggcgtt ttctgtttca atccgcccca
    11521 tccgccaaac ataaaaatgc cgtctgaaac cgttgcaagt ttcagacggc atttcgctta
    11581 tccgcttatt ttttgctcaa agtcagcccc gtccaaccga ataacagggt caaagccaaa
    11641 ctcaaatagc agaaaaaggc atacggcaga tattcccaaa ccggcacgcc cagcgcgtgg
    11701 ctgatgaaca cgccgcatac gctccacggt acgagcgggt taatcaccgt ccccgcatct
    11761 tccagcgtcc gcgacagatt gcgcgaatgc agaccgagct tatcgtaaac gggtttgaac
    11821 gtttcacccg acaacaaaat actcaaatat tgctcgccga tcaggaaatt aaccccgacc
    11881 gaagtcatgg caacgctgaa cgtcgcgcgt ccggcattcg tcaagaaggt acggatggcc
    11941 tccaacaggg aaggaatcac accgagcgca aacaacagtc cgcccaaact catcccgaga
    12001 atcacgatgg tttgcgtgaa aaacatactt tccaagccgc cgcgcgaaat gagtttgaca
    12061 acatctttaa acgcttcgcc ttcgagtttg taaccgccgt aaaaccacgc gccgagctga
    12121 cgcagatcgg gcgtgctgtg cagatacgtt acggcaacgg caaccatgac ggtaaagagc
    12181 atggcgacca cggcgttgac gcgcatcaat gccaaaatga ccaacagcgc aaacggaatc
    12241 agcgaatagc cgtgcaccaa tcccgtggct tcaagctggc tgcggaagga ttcgacgctg
    12301 ttcaaatcct gcgcggcgac actgggaaga agccacagca tcagtgccgc actgatgagc
    12361 cacgcgggaa tggtggtgta catcatattt ttgatgtgct caaacaggtc gatgccgacg
    12421 atggacgcgg aaatgcccgt ggtgtcggaa agcggggaca ttttgtcgcc gaaaaacgca
    12481 ccggaaacaa tcgcgcccgc cgtcatcgcc atatcggcat gaaacgccgc cgccatcccc
    12541 ataaaggcaa cgccgacggt ggcgcaggtg gtcaggctgc tgccgatgga tacgccgatg
    12601 acggaacaca gcgcgaaagc ggaaaaataa aaataagtcg gggaaatcag cccgaaaccg
    12661 taatacatca gcgtcggaat cgcaccgctc atcatcagcg cgctgaccat cagcccgatg
    12721 aagaaaaaca ggtaaatcgc gcccataccc tgattcaacg cgcctatcat gccctgctgc
    12781 atatcgttgt atttcaaacc gcgcgccaag ccgtacaaaa tcagcacgac gatggcggca
    12841 ataatggaca tatgcggcag ccactccaat gaaatgatgg tatagcccat cgcggcaatc
    12901 agagcgacga cgacggcaag tgcctcaccg cgcggcatat cgagtaagga tttgaaagcg
    12961 aacatgattc tttgcccgat ttgatataaa gttaacggat tctaacacac tataccgaat
    13021 ccgcccgacg ttttcagacg gcattttagc gttttacaaa agccggacag aaacaaaacg
    13081 gcaattttct caatttaaat taattttttt gaaatataat tttttatttt tttgtttgtt
    13141 gacatataag acttttatgg attgaagtat taaaacctcg gcacacacgc cgcaaacacc
    13201 ggtacgcaaa aatgccgtct gaaagatgtt cagacgacat ttctcaagaa aaagattatt
    13261 tgtccgccct atatccgtac accgcaaaaa acacgatata cagataacag atgacggaaa
    13321 cgacaaacga agacatcagg gtgtaagtat ctgccaccca gccctgcacg acaggaacga
    13381 ccgcaccgcc gacaatcgcg gtacacagta caccggaagc cgcgttggta aattttccca
    13441 atcctttggt tgccaaagag aaaatcgtcg ggaacataat cgagttgaaa aaaccgatgg
    13501 caagcagcga ccacatcgcc acatccgcat tgccgctacc cgtcgccatc gcgacggcaa
    13561 gcagtacgac cgcagccgat gcgttaaacg ccaaataacg gttgggcgcg aatttcgcca
    13621 tcaccgccga accgaggaaa cgtccgacca tcgcgccgcc ccaatagaac gacaggtaat
    13681 gcgcggcaga agcatgatcc agccctttca gataacccaa tacgttgacc atcaacgaac
    13741 cgatagacac ctccgcgccg acatagcaga aaatgcctgc cgtgccgaac acgagatgtt
    13801 tgtattgcca tacgctggtt ttgccgtcgt ggttgtgcgc gctttcctcg gcggcaattt
    13861 tgcgcgcgtc gggcagccgg atcattttca cgaaaacggc aaggataatc agcagccccg
    13921 ccagtcccaa atagggaatc tgtacggaag aaatctgttc cgccttgctg acggtttggg
    13981 ttgcgtccgc cagaatcagg aacgcgccga tttgcggcgc aatggtcgta cccaaagcgt
    14041 taaacgcctg aaccagcgtc agtgttgccg attccttgcc aggtttcgcc agcagggtaa
    14101 cataaggatt accggcgacc tgaagcagcg ttacgccgga agccaaaata aacaacgcgc
    14161 ccaaaaatac cgcgtaagaa tggctacccg cagcaggata aaacagcagg catccgaccg
    14221 ccgtcagcag aaagccgccg ataacgccgt ttttgtagcc gactttgccg acaaaagccc
    14281 ccatcgggat ggacatcacc gcataggcgg taaagaaaca gaattggatc agcatcgcct
    14341 gaacgtaaga caggtcgaaa atttctttca aatgagggat aaggatgtcg ttcatgcagg
    14401 taataaaacc catcatgaag aacagcgtgg tcaagacgac caatggggac gtatgattgt
    14461 tttgtgattg cgcagacatg atttgccttt cgtggtgtcg gaatattacc cgtttcaaga
    14521 aaatagaaat tgacatttct taacggcaat gcaaatcata ctgatatttg aacattttgc
    14581 aaactttatc ggatgcaggc aatgcgccga tctgaaattc aagcagcaga aattaatatt
    14641 ttacagttgc ttgcatagat tttaatagat tttaaaaaat tccggcggac ttgcaaaaac
    14701 cgttcccaat aaaatgaaaa tccgccgccg tatccggaaa attcggaaat gccgcatcgt
    14761 ggcatcaaaa ccggagcgga aagaatgtcg tatgcccgac aaaaaagggg catacggctg
    14821 tgccgtattt tttgcaccgt cctccgccct tcggcggggg cgtgaaaata tagtggatta
    14881 acaaaaatca ggacaaggcg acg
//
LOCUS       DAWKOG010000034        14885 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.034, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000034 DAWKOG010000000
VERSION     DAWKOG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14885
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.034"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            319..882
                     /locus_tag="WJK66_000801"
     CDS             319..882
                     /locus_tag="WJK66_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ7578544.1"
                     /translation="MAIELNGNWTKGFALDHHMQKSILLGYDELGHPHFDSKRSVIGE
                     WVYQLKYRNQTQNVSLLVDYILQKFSGLESINLIVPAPFTTERINQPVQLIAKELSDR
                     LNIPYSPILRKSSSHTPLKNIEEKSEKLEILNNSITIDNIDLSNKNILVIDDLFDSGA
                     TLEISTEKLFSKNARSVIVLAMTKTRG"
     gene            885..1433
                     /locus_tag="WJK66_000802"
     CDS             885..1433
                     /locus_tag="WJK66_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578545.1"
                     /translation="MRTIFFSGSRSISRLNPQIRERINNILSNNFDIVIGDANGADKA
                     IQKFLQEQDYANVHIYFSGKIYRNNVGNWQFVQVDSKGTGRVFYTAKDKKMAEIADYG
                     FILWDGKSIGSLNNIAELLQLNKPSLVYHSQTKEFFKIKSSADLENILSNIEDDVLAS
                     ILEKGNTFLKSYVTKQPSLIQE"
     gene            1440..4655
                     /gene="carB"
                     /locus_tag="WJK66_000803"
     CDS             1440..4655
                     /gene="carB"
                     /locus_tag="WJK66_000803"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEZ7578546.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFKLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSSDKAEIRRELANPGPERMLFVADAFRAGFTLEEIHEI
                     CAIDPWFLAQIEDLMKEEKAVSDGILSDLDFAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEELQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQVLGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRS"
     gene            complement(4882..5781)
                     /gene="crgA"
                     /locus_tag="WJK66_000804"
     CDS             complement(4882..5781)
                     /gene="crgA"
                     /locus_tag="WJK66_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HEZ7578547.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMP
                     MVLHLLAPLATKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSTEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSDKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            6020..6598
                     /locus_tag="WJK66_000805"
     CDS             6020..6598
                     /locus_tag="WJK66_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ7578548.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYIDEVLTAGHGKLYQSDGRHSVN
                     PTEGYGTGGLLQGKKHMLSLTWNAPIEAFTREGDFFEGKGVDVLYMHFHKANEFLGMT
                     RLPTFLCNDVVKNPQVEKYLADYQAHLEKVFG"
     gene            complement(6807..8192)
                     /gene="pntB"
                     /locus_tag="WJK66_000806"
     CDS             complement(6807..8192)
                     /gene="pntB"
                     /locus_tag="WJK66_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HEZ7578549.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIEPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFVGINGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TLSSGSQEIGEYREVKAADIAEMLKGANNVIITPGYGMAVAQAQYPVAEITELLRKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(8258..8641)
                     /locus_tag="WJK66_000807"
     CDS             complement(8258..8641)
                     /locus_tag="WJK66_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEZ7578550.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRDFLARTQ
                     GTAARAHAAQQNGFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFACTVGLFVAAA"
     gene            complement(8645..10186)
                     /locus_tag="WJK66_000808"
     CDS             complement(8645..10186)
                     /locus_tag="WJK66_000808"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEZ7578551.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWACPLIYKVNAPSEGELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDVVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTHDGEITFPPPPIQVSAQPQQTPSEKAAPAA
                     KPEPKPVSLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQISQGNGFVTLLAFIAILIASINIFGGFFVTRRMLN
                     MFRKG"
     gene            10476..11309
                     /gene="serB"
                     /locus_tag="WJK66_000809"
     CDS             10476..11309
                     /gene="serB"
                     /locus_tag="WJK66_000809"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEZ7578552.1"
                     /translation="MPHALVLQFPSAEALPSDFPSRLPEPDYADEKRMRFIVEEGFSL
                     SEADAALLGSRQIDHAVLPDMDFDELGLIVSDMDSTLITIECVDEIAAGVGLKDKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDEQILADVYENVLKLSPGAEFLLDECKRHDVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANVLEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPMLKEAGIGVAYRAKPKAQAAADACINFGGLERVRGLF
                     R"
     gene            11373..12596
                     /locus_tag="WJK66_000810"
     CDS             11373..12596
                     /locus_tag="WJK66_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ7578553.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIQHTAYGYGADGAY
                     ISFREGVAQASGMRRVAMLTLCGAIAGGGWWLLKALGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFASAGGKRLGLDEGEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGMWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLAV
                     NTSLLWFSAVIGPILGATAVFFQSTAQKFPFIKRDNIKIIPLAVCMFALIGAIAVWFP
                     EVLGNGKAGNQLTFGGLTDWQHSLELTAVKWLVVLMALAAGAYGGLITPSMMLGSTIS
                     FAAAAAWNSVFPEMPSESAAVVGAAVFLGVSLNMPLTAAVFVLELTYAPAALLMPLCT
                     GMAGAVWTARKMGCK"
     gene            12760..14340
                     /gene="purH"
                     /locus_tag="WJK66_000811"
     CDS             12760..14340
                     /gene="purH"
                     /locus_tag="WJK66_000811"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEZ7578554.1"
                     /translation="MPSIKRALISLSDKTGAVEFAQTLHKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLPEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDEVLSGTPEIGEFPSQFNQSW
                     IKVQDMRYGENPHQRAAFYRDIDPAAGSLSAYKQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYVTDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDIHR
                     LNRADLKVVSKRQPTEQEWNDLMFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDAGLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTDVRHFRH"
BASE COUNT         3622 a         3890 c         4004 g         3369 t
ORIGIN      
        1 ccctccccca cgggggaggg ggcaggttgc agcggatttt gcggttgcag gcggtttgag
       61 aaagaatgcc cgaaatatca acagtgggaa tttttcaggc agcctttatc gcaaggcaga
      121 tgtaacaaac gccgcgagcg ttttttcaga cgacctttga atccgtcggc agagtgtgtg
      181 ccgcaaggcg cgcatgcggt gggttggggt tacagggaaa atgaagaacg cgtgcgtgcg
      241 taccgcacat accctacatg cgggctacgg cttgctagga ttgctcgtta tacttaattt
      301 tgacaaggag tcaatgaaat ggcgatagaa ctaaatggta actggacaaa aggatttgct
      361 ttagaccatc atatgcaaaa aagtattttg ttaggttatg atgaattagg gcatcctcac
      421 tttgatagca agagaagtgt gattggagaa tgggtttatc aattaaaata tcgtaatcaa
      481 acccaaaatg tttctttgct ggtagactat attttacaaa aatttagtgg tttagaatct
      541 ataaatttaa ttgttcccgc cccttttaca acagagcgta ttaatcaacc agttcaatta
      601 attgccaaag aactaagtga tcgcttgaat attccatatt ctcctatttt aagaaaatct
      661 agctcacaca caccactcaa aaatattgaa gaaaaatcag aaaaactaga aatactgaat
      721 aattccatca caattgacaa tatagatcta tccaataaga atattttagt aattgatgat
      781 ttatttgata gcggagcaac attggaaata tcaactgaaa aattgttttc aaaaaatgct
      841 agatctgtaa ttgttttagc aatgacaaag actagaggat aaaaatgcgc actattttct
      901 tttctggttc acgcagcatt tcacgactaa atccacaaat acgagaaaga attaacaata
      961 ttcttagcaa taattttgat attgttattg gagatgctaa tggggctgat aaagctatcc
     1021 aaaaatttct acaagaacaa gattatgcta atgtgcatat ttacttttct ggcaaaattt
     1081 accgaaataa tgtggggaat tggcagtttg tccaagtaga cagcaaagga actggaaggg
     1141 tattttatac agcaaaagat aagaaaatgg cagaaattgc tgattatggc tttattttat
     1201 gggatggtaa gagtattggt tcattaaata atatagcaga acttttacag ctaaataaac
     1261 cttctcttgt ataccattct caaacaaaag aattctttaa aattaaatca agcgcagatt
     1321 tagagaatat tttatctaac atagaagatg atgttttagc ttcaatttta gaaaagggaa
     1381 atacttttct taaaagctat gtaacaaaac aaccttcttt aattcaagag taaccaccca
     1441 tgcccaaacg taccgaccta aaatccatcc ttatcatcgg cgccggccct atcgttatcg
     1501 gtcaggcctg cgaatttgac tattcgggcg cacaggcctg taaggctttg cgtgaagaag
     1561 gctataaagt cattctggtg aattccaacc ccgccaccat catgaccgac cctgaaatgg
     1621 cggatgttac ctacatcgag ccaattatgt ggcagacggt ggagaagatt atcgccaagg
     1681 agcgtcctga tgcgattctg cccacgatgg gcggtcagac cgcgctgaac tgtgcgctgg
     1741 atttggcgcg caacggcgtg ctggcgaaat acaatgtcga gctgatcggc gcgacggaag
     1801 acgcgattga caaggcggaa gaccgcggtc gttttaaaga agcgatggaa aaaatcggcc
     1861 tctcctgccc gaaatctttt gtctgccaca cgatgaacga agctttggca gcgcaagaac
     1921 aggtcggctt ccctaccctg attcgtccgt ctttcacgat gggcggttcg ggcggcggca
     1981 ttgcctacaa taaagatgag tttttggcga tttgcgaacg cggtttcgat gcgtcgccta
     2041 cgcacgagct gctgattgag cagtctgttc tcggctggaa agagtacgag atggaagtgg
     2101 tgcgcgacaa gaacgacaac tgcatcatta tctgctcgat tgaaaacttc gacccgatgg
     2161 gcgtgcatac gggcgactcg attacggttg cgcctgcgca aacgctgacg gacaaagaat
     2221 accaaatcat gcgtaatgct tcgttggcag tattgcgcga aatcggcgtg gataccggcg
     2281 gctcgaacgt gcagtttgcg gtgaaccctg aaaacggcga gatgattgtg attgagatga
     2341 acccgcgcgt gagccgttcg tccgcgctgg cttccaaagc gacgggcttc ccgattgcga
     2401 aggtggcggc gaaactggcg gtcggcttta agctggacga gttgcgcaac gacatcaccg
     2461 gcggcaaaac ccccgcgtca ttcgagccgt ccatcgacta tgtggtaacc aaaatcccgc
     2521 gttttgcgtt tgaaaaattc cctgccgcag acgaccgcct gaccacgcag atgaaatccg
     2581 tgggcgaagt gatggcgatg ggccgcacga ttcaggaaag cttccaaaaa gccctgcgcg
     2641 gcttggaaac aggcttgtgc ggcttcaatc cgagaagctc cgacaaagcg gaaatccgcc
     2701 gcgaactggc aaaccccggc cccgaacgta tgctgtttgt ggcagacgcg ttccgcgccg
     2761 gcttcacgct ggaagaaatc cacgaaatct gcgccatcga cccttggttc ctggcgcaaa
     2821 tcgaagactt gatgaaggaa gaaaaagcgg tttcagacgg cattttgagt gatttggatt
     2881 tcgccgccct acgccgtctg aaacgcaaag gcttctccga caaacgtttg gcacaattgt
     2941 tgaacgtaag cgaaaaagaa gtgcgcgaac accgctacgc gctgaagctg catccggttt
     3001 acaaacgcgt tgacacctgc gccgccgagt tcgccaccga aaccgcctat ctttactcca
     3061 cttacgaaga agaatgcgaa tcccgtcctt ccgaccgtaa aaaagtgatg attctcggcg
     3121 gcggcccgaa ccgcatcggt cagggcatcg agtttgacta ctgctgcgtt cacgccgcac
     3181 tcgccctgcg cgaatcgggc tttgaaacga ttatggtgaa ctgcaaccca gaaaccgtgt
     3241 ccaccgactt cgacaccagc gaccgcctct atttcgagcc gctgacattg gaagacgtgt
     3301 tggaaatcgt ccgcaccgaa aacccgtggg gcgtgattgt gcattacggc ggtcaaaccc
     3361 cgctgaaact cgccaacgcg ttggttgaaa acggcgtaaa catcatcggc acgtccgccg
     3421 acagcatcga tgccgccgaa gaccgcgaac gcttccaaaa agtgttgaac gacttaggcc
     3481 tgcgccaacc gcccaaccgc atcgcccaca acgaagaaga agccctcgtc aaagccgaag
     3541 aaatcggtta tccgctggtc gtgcgcccgt cttacgtcct cggcggacgc gcgatgcaga
     3601 ttgttcactc tgccgaagag ttgcaaaaat acatgcgcga agccgtgcag gtttccgaag
     3661 acagccccgt gttgctcgat ttcttcctca acaacgcgat tgaagtcgat gtggactgcg
     3721 tttcagacgg caaagacgtg gttatcggcg gcatcatgca gcacgtcgaa caggcgggca
     3781 tccactccgg cgactccggc tgctcgctgc cgccctactc cttaagcgaa gaaatccaag
     3841 acgaaatccg ccgccaaacc aaagcgatgg cgtacgcgct gggcgtggtc ggactgatga
     3901 acgtgcagtt tgccgtacaa gacggcgtgg tgttcgtatt ggaagtgaac ccgcgcgcca
     3961 gccgcaccgt gcccttcgtc tccaaagcca ccggcgtgcc gctcgccaaa gtcggcgcgc
     4021 gctgcatggc aggcatttcc ctgaaagaac aaggcgtgga aaaagaagtc gtccccgatt
     4081 tctatgccgt taaagaggcc gtgttcccct tcatcaaatt cccgggcgtg gatacgattt
     4141 tgggaccgga aatgcgctcc accggcgaag tgatgggcgt gggcgcaagc ttcggcgaag
     4201 cctactacaa agcccaactc ggcgcaggcg aacgcctcaa cccgaccggc aaaatcttcc
     4261 tctccgtgcg cgaagaagac aaagaacgcg tcattaaaac cgctaaaaac ttccaagttt
     4321 taggctacgg catctgcgcc acgcgcggca cggcgcaata cctgaccgaa cacgggctga
     4381 ttgtacaaac catcaataaa gtccccgaag gccgcccgca catcggcgac gcgctgaaaa
     4441 acggcgaaat cgcactggtc gtgaataccg tttccagcga tccgcaatcc gtgtccgaca
     4501 gccacatcat ccgccaaagc gcattgcagc aacgtgtgcc gcaatacacc actactgcgg
     4561 gcggcgaagc gatgagcgaa ggcgcgaaaa gccgcgacca cttgggcgtg tacagcgttc
     4621 aagaactgca cgggcgtttg aaaaaccgca gctgatgcct gaatcgggtt gaaaatgccg
     4681 tctgaaacct ttttttacgg tgtcagacgg cattttgtca tttggaaagc cgatgttgcc
     4741 acacacaagc cgtacataag gaacagccct atcacgctcc ccatatagac tgccattgcc
     4801 gccgactata catcatctta tttatttttt ctcaaagtta ttaagtgagt aaaaacaatt
     4861 ttatggcagg tttttataga attatccaca gagattgttt cccagttcct ccactaaaaa
     4921 atccaaaaat acgcgtaagc ggagattgac ggctttatcg ctgtaataaa cagcattaaa
     4981 ggggtgtgtt ttatcggagg tttgttcggc gagcagggga attaactttc cttcagcgat
     5041 gtcgttgtca accaaaaaat ctgataagca agcaataccg caacctgaaa ggcacaacga
     5101 gcgtaagatt tcaccgctgc tggcggtaaa gtgcggtgaa atcttatagg gatttccctg
     5161 cgcatctaaa accgcccatg tatttagaga accgggttcg gtgaagccta aacattggtg
     5221 gccggcaagc tcttctgtag attgcggcgt gccgtgtttt gccaggtatt caggactggc
     5281 gattacgcgg aagcggctgt caaacagatg gcgtgcacgc agcccggaat cgtccaattc
     5341 tccggcccgt aaggcaatat cgactttgcg ttcaatcaga ttgatatagc cttcggaaga
     5401 aacgagcgaa agtcggatat gcggatagcg ttcgttgaat tttgttgcca gcggcgccag
     5461 cagatgcagc accatcggca tcgcggaatc cacgcgcaac acgccttgcg gtatttcgtg
     5521 cactgccagc atttcggttt ccgccgctgc catttcttgc aggattctct gcgcgcggcg
     5581 gaaatattgc gcgccttctt ccgtcagact gagttgccgc gtggtgcggt tgagcaggtt
     5641 cacacccaac ttttcctcca gccgtttgac gatgcggctt acggcagaat ttgccatcgc
     5701 caactgctcc gccgcacggc tgaagctgcc gctttccacc acttgaacaa atacggtcag
     5761 ttcttctgaa ttggttttca tcgtgtttcc ttttcggttg gaaccccgcc ctttagggcg
     5821 gcaggatcag actttatttg ggaggggtgt aaccccttcc gaatcaggac ggcacacagg
     5881 gcggtgcttt atgtgccatc ccgtgtgttg gaacatctga ttatttcatt tgacgcaaaa
     5941 gtgttttctt atttttgcac ttttaaatta taaagtaaaa cggcacaata cattcatcaa
     6001 ttcacaaacg aggtaacaaa tgaatatttt attattagac ggcggcaaag cgttcggaca
     6061 ttctcacggc gggttaaacc gcacgcttca caaaaaagcg aaagaagttt tgaccgcgct
     6121 cggacacaat gttcaagaaa ccgtgattga tgccggctat gatgttgagg cagaaatcga
     6181 aaaattcgtt tggatggatg ctgtgatttg gcagatgccg ggctggtgga tgcacgagcc
     6241 ttggacagtg aaaaaataca tagacgaagt attaaccgct ggacacggca aactctacca
     6301 aagcgacggc agacacagcg tcaatccgac tgagggctac ggcacaggcg gcttgttgca
     6361 aggcaaaaaa catatgcttt cactgacttg gaatgcgccg attgaggcgt ttacccgcga
     6421 aggcgatttc tttgaaggca aaggcgttga tgttttgtat atgcacttcc acaaagccaa
     6481 cgagtttttg ggtatgaccc gcctgccgac attcttatgt aacgatgtgg ttaaaaatcc
     6541 gcaagtggaa aaatacttgg cagattatca ggcacacttg gaaaaagtgt tcggctaatt
     6601 aaaaatccat cttcaacacg gagatggatt ttgtttgttt cgttgatttt gtgtcagttt
     6661 cagatgtagg tgcttattcg gacgggccgt ctgaaaatgt ttgccccaat gcaaaaaaaa
     6721 tcactgcaaa ccttcataaa cggggtttgc agtgattttt tcaaatcaaa cagattgaaa
     6781 acctgcgccg aattgttcag acggcattat tttttcagtt cggacagaat gtcatccacg
     6841 gttttcttcg catctccgaa acacatcacg ctgttttcgt tgaagaacag tgggttttgt
     6901 acacctgcgt agccggtatt catcgagcgt ttgaagacga cgacttcttt tgccttccac
     6961 acttccaaca cgggcatacc cgcaatcggg ctgttcgggt cggtttgggc ggcggggttg
     7021 acggtgtcgt tcgcaccgat gaccaagacc acatcggttt cggggaagtc gtcgttgatt
     7081 tcgtccattt ccaaaacgat gtcgtagggg actttggctt cggcgagcag tacgttcata
     7141 tgaccgggca ggcggccggc gacggggtgg atgccgaagc gtacttcggt gccgttttta
     7201 cgtaaaagct cggtgatttc ggcaacgggg tattgcgctt gtgcgactgc cataccgtag
     7261 cccggggtaa tgatgacatt gtttgcgcct ttcagcattt cggcaatatc ggcagctttg
     7321 acttctcggt attcccctat ctcttggctg ccggaagata atgtgccgct gtcgctgccg
     7381 aaaccgccgg caattaccga gacaaacgag cggttcatgg ctttgcacat aatgtaggac
     7441 agaatcgcgc cgcttgagcc gaccagcgcg ccggtaacga tgagcaggtc gttggagagc
     7501 atgaagcctg ccgctgcggc cgcccagccg gagtaggagt tgagcatgga cacgaccacg
     7561 ggcatatctg cgccgccgat ggaggcaacc aagtgccagc cgaatgcgag ggcaatcagg
     7621 gtcataatca gcaggatgaa gccgctaccg ttaatgccga cgaatacgag cagcaacaca
     7681 aacgatacgg cgagcgccag tgcgttgagc ttgtgtttgg cgggcagttg cagcgggctg
     7741 ctgctgattt tgccgttgag tttgccgaat gcgaccagcg agccggtaaa ggttaccgcg
     7801 ccgatgaaaa tgcccaaata gacttcgacc agatggatgg tgtgcatatc gtgcgaaacg
     7861 ttgcccggct cgatatagct gttgaagccg accaaaaccg ccgctaggcc gacgaagctg
     7921 tgcagcaggg caatcagttc gggcatttcg gtcatttcca cctttttggc tttgtagatg
     7981 ccgattgccg cgccgatgag catggcgatg atgatccagc ccagtccgtg ggtattgtcg
     8041 gaaaaaacag ttacaaaaag ggcgaccgcc ataccggcga taccggaata gcagccctgt
     8101 ttggcggttt cctgtttgga cagccccgcc agtgagaaga tgaataaaat tgcggcaacg
     8161 atatacgccg ctgttacgag tcctgaagac atggaaattc tccgattttc gatgatttgt
     8221 ttttaatgcc gtctgaactg ttcagacggc atctgtttca agcagccgcg acaaacagcc
     8281 cgacggtgca ggcaaatccg cccgcccaca tcagcgagcg caatgctgct ttgtctgcga
     8341 tgtagcacca gataaaggcg aggcggaaca ggatgaacag gccggcaagc gtgttgacgg
     8401 ttgcttgtcc ggcattgccg gttgcgtgtg ccgtcaaaac ggcggcggca aacggtgcaa
     8461 aggcttcaaa accgttttgc tgcgcggcgt gggcacgggc ggctgtgcct tgcgtgcgcg
     8521 ccagaaaatc gcgcggattg tggttgtctt taaaccggaa tccgcccgct tttttggcat
     8581 atgccgcaca aaaaagcggc aataagcagg caatcaggat gcaccaatag gcaaaagtca
     8641 tgctttatcc tttcctaaac atattcaaca tgcggcgcgt tacgaagaag ccgccgaaga
     8701 tattgatgct ggcaatcagg atggcgatga aggcaagcag ggtaacgaaa ccgttgcctt
     8761 ggctgatttg cagcagcgcg ccgacgacga tgatgccgga gatggcgttg gttaccgaca
     8821 tcagcggtgt gtgcagcgag tggctgacgt tccaaacgac atagtagccg atgacgcagg
     8881 caaggacgaa cacgataaag tggttcagga atgctgcggg tgcgaccgcg ccgacccaca
     8941 gcaccaatac ggcggcgatg acggcgggcg cgagtttttt ccacagggaa acaggtttcg
     9001 gctcgggctt ggcggcaggc gcggcttttt cagacggcgt ttgctgcggc tgggcggaaa
     9061 cttgaatcgg cggaggcggg aaggtgattt cgccgtcgtg ggtaacggtc atgttgcgga
     9121 taatcacgtc ttcgaagtcc aacgtgattt cgccgtcttt gttcgggctt aacagcttgg
     9181 tcaggttgac caagttggtg gcgtaaagct gggaagactg tccggcaagg cggtttgcca
     9241 tgtcggtgta gccgatgatt ttcacgccgt tgccggttac ggacaattcg cccgggcggg
     9301 tgagttcgca gttgccgccc gtcgccgccg ccaaatcgac gatgacggag ccggatttca
     9361 tgctttccac catttctttg gtaatcagct tgggcgcggg tttgcccgga atggcggcgg
     9421 tggtgatgat gatgtccact tctttcgcct gctcggcaaa gagcttcatc tcggctgcga
     9481 taaattcgtc gctcatcact ttggcgtagc cgtctccgct gccgcccgat tcttgtggga
     9541 agtcgagttt caggaacttg ccgcccatcg attcgatttg ttccgccact tccaagcggg
     9601 tatcgaacgc gcgcaccact gcgccgagcg agtttgccgt accgatcgcc gccaaacctg
     9661 ccacacctgc gccaatcacc aaaacctgcg cgggcggcac tttgccggcg gcggtgattt
     9721 ggccggtgaa gaaacggccg aaggcgttgg cggcttcaat tacggcgcgg tagccgctga
     9781 tgtttgccat cgaagacaaa gcgtccaaag cctgcgcgcg cgaaatgcgg ggcaccacgt
     9841 ccatcgccag cgcgttcact ttcttggcgc gcaaggcttc gaccaaagcc tcgttttggc
     9901 gcggccacag gaagctgacg atggtttgac cttcgttcaa aagcggcagc tcgccttcgg
     9961 acggcgcatt gaccttataa atcaaagggc aggcccaaac cgccgctttg tcggcaacgg
    10021 tcgcgcctgc tgtttggtaa gcggcatcgt ccaaacttgc cgccaaacct gcaccgcttt
    10081 cgacaacggt ttcaaagccc agtttgccca gcagggcgac ggtggcgggc gtgcaggcga
    10141 cgcgggtttc gccggataat gactcgcgtg ggataccgat tttcatctct gaatcctttt
    10201 tcgggtagtt tatatgtatc gtgggttaaa tttaaatcgg ggcagggcgg agcaacgccg
    10261 taccggttta aaaccgattc acttcaaatg ttaatatatt ttagataatc cccttataac
    10321 gaattttcat caggctggca atagttgcgg cattttcccg tgttgtccga cacatactgc
    10381 cgtttgcgtt tgaagtcatt ttccgattgc agtcccgccc gccgtaacat tctttgccgt
    10441 ttcactatat aatccgcatt ttttgagtta ccgccatgcc gcacgccctc gtcctccaat
    10501 ttccctccgc cgaagccctg ccttccgact tcccctcacg cctgcccgaa cctgattacg
    10561 ccgatgaaaa gcgtatgcgt tttatcgttg aagaagggtt ttctttaagc gaggcggacg
    10621 cggcgttgct tggcagccgt caaatcgacc acgccgtgtt gccggatatg gatttcgacg
    10681 aactcggttt aattgtcagc gatatggatt cgacgctgat taccattgaa tgcgtcgatg
    10741 aaattgcggc aggcgtgggt ttaaaagaca aagtagcgga aattaccgag cgttcgatgc
    10801 gcggcgaact cgatttcgaa cagtctttac gcagccgcgt tgcgctgttg gcgggattgg
    10861 acgaacagat tttggcggac gtttatgaaa acgttttgaa gctctcgccc ggtgcggaat
    10921 tcttgttgga cgaatgcaaa aggcacgatg tgaaattcct gctggtgtcc ggcgggttca
    10981 cattttttac cgaaaggctg caacaacgac tcggcttcga ataccaacac gccaatgttt
    11041 tggaaattga aaacggcagg ttgaccggtc gtctgaaagg cagaatcatc gacgcgcagg
    11101 caaaggcgga tttattgcgc gaataccgca gccgcctcgg attgcagccg catcaggtgt
    11161 tggcgatggg cgacggtgcg aacgatattc cgatgctcaa agaagcgggc ataggcgtgg
    11221 cttaccgtgc caaaccgaaa gcgcaggcag ccgccgatgc ctgtatcaac ttcggcggtt
    11281 tggagcgtgt acgcggcctg ttcaggtagg cggataggaa acggatgccg tctgaaaggt
    11341 tttcagacgg catttgaacg tcaggaacga cagtgggacg cagaaaactt tggtttgccc
    11401 tggcagcagc gggcgttatc ggcggtttag tcggcattgt gctgacagaa ctgatgcact
    11461 tcatacagca tacggcatac ggttatggcg cggacggtgc gtacatctcg ttccgcgaag
    11521 gtgtggcaca ggcttccggt atgcggcgcg tcgccatgct gacgctgtgc ggcgcgatcg
    11581 caggcggcgg ctggtggttg ctgaaagcct tgggcaagcc gcaaatcgaa atcaaagccg
    11641 ccttgaagca gccgttgcag gggctgccgt ttctgacgac ggttttccat gttctgctgc
    11701 aaatcataac ggtcggactc ggttcaccgc tcggacgcga agtcgcgccg cgcgaaatga
    11761 ccgccgcgtt tgcttcggca ggcggcaaac gcttgggttt ggacgaaggc gaaatgcgcc
    11821 tcttgattgc ctgtgcgtcc ggcgcgggtt tggcggccgt gtataacgtg ccgctcgcct
    11881 ccacactttt catcctcgaa gccatgctgg gcatgtggac gcagcaagcc gtcgccgccg
    11941 cgttgctgac ttcggtcatt gccaccgccg tcgcacgcat cggcttaggc gacgtgcagc
    12001 aatatcatcc cgccaacctt gccgtcaaca cctcattact ttggttttcc gccgtcatcg
    12061 gcccgatact gggcgcaacc gccgtttttt tccagagtac cgcccaaaaa ttccccttca
    12121 tcaagcgcga caacatcaaa atcattccct tggccgtctg tatgtttgca ctcatcggcg
    12181 cgattgccgt ttggtttccc gaagttttgg gcaacggcaa agcaggcaac caactgactt
    12241 ttggcggttt gaccgactgg caacacagcc ttgagttgac cgccgtcaaa tggctggtcg
    12301 tcttgatggc gctggcggca ggcgcatacg gcggactgat tacgccgtct atgatgctcg
    12361 gcagcaccat ttcttttgca gccgccgccg cgtggaacag cgttttcccc gaaatgccgt
    12421 ctgaaagcgc ggcagtcgtc ggcgccgccg ttttcctcgg cgtttccctc aatatgcctc
    12481 tgaccgccgc cgtctttgtt ttggaactca cctacgcccc cgccgctttg ttgatgcctt
    12541 tatgtacggg tatggcgggc gcggtatgga cggcaaggaa aatgggatgt aaataaccga
    12601 aggcaaaatg ccgtctgaaa ccggttttca gacggcattc ctttggggat tgtcaacaat
    12661 atctgcaaaa acctactgcc ggaaagtgga aaatagcgga taataccgcc ccgacaacat
    12721 taccgtttcc catcccaacg tcagaaaaag gaaatcccta tgccttccat caaacgcgcc
    12781 ctgatcagcc tgtccgacaa gacgggcgca gtcgaatttg cccaaaccct gcacaagctc
    12841 ggtgtcgaaa tcctctctac cggcggcacg gcgaagctgc ttgccgatgc gggcgttccc
    12901 gtgattgaag ttgccgacta caccggtttc cccgaaatgc tcgacggccg cgtgaaaacg
    12961 ctgcatccga aaatccacgg cggtattttg ggccggcgcg atttgcctga acacgtcgcc
    13021 aagatggaag aacacggcat cggcaacatc gacctcgtgt gcgtcaacct ttatcccttc
    13081 gccgccacca tcgccaaacc aaactgcaca ctggaagacg cgattgaaaa catcgacatc
    13141 ggcggcccga ccatggtgcg ttctgccgca aaaaactgga aacacgtcgc catcgttacc
    13201 gacaccgccg acttccccgc catcgccgcc gaactcgaag ccaacaacgg cgcgttgagc
    13261 gacaaaaccc gcttcaacct gtcgcgcaaa gcgttcagcc acaccgccca atacgacggc
    13321 atgatttcca actacctgac ctcgctttca gacgaggtct tgagcggcac gcccgaaatc
    13381 ggtgaattcc cgagccagtt caaccaaagc tggattaaag tgcaagacat gcgctacggc
    13441 gaaaacccgc atcagcgcgc cgcgttctac cgcgatattg accccgccgc aggcagcctt
    13501 tccgcctaca aacaactgca aggcaaagaa ttgtcttaca acaacatcgc cgatgccgat
    13561 gccgcatggg aagccgtcaa atcctttgac gcgccagcct gcgtgatcgt caaacacgcc
    13621 aatccgtgcg gcgtcgccgt tgcagccgat acattgaccg cctacaaact cgcctacgtc
    13681 accgacacca ccagcgcgtt cggcggcatc atcgccttca accgcgaagt ggacggtgaa
    13741 accgtcaaac agattaccga caaccagttt atggaagtct tgatggcgcc gaagtttacc
    13801 gccgaagccc tcgaaatcgc cgccgccaag aaaaacgtgc gcgtattgga agtgccgctt
    13861 gaggcaggcg caaaccgctt cgaactcaaa cgcgtcggcg gcggactgtt ggtgcaaacc
    13921 cccgatattc accgcctcaa ccgcgccgat ttgaaagtcg tttccaaacg ccaaccgacc
    13981 gagcaagaat ggaacgacct gatgtttgtt tggaacgtcg caaaatacgt caaatccaac
    14041 gccatcgtct tcggcaaagg cggtcaaacc tacggcatcg gcgcaggcca aatgagccgc
    14101 gtggacagta cccgcatcgc cgcccgcaaa gcgcaggacg caggcttgga cttaaacggc
    14161 gcatgtgccg cctctgatgc tttcttcccc ttccgcgacg gcgtggacgt gattgccgag
    14221 cagggcatca aagccatcat ccatccggca ggttcgatgc gcgatcagga agtcttcgac
    14281 gcagcggacg aacacggcat cgccatggtc gtaaccgacg tgcgccactt ccgccattaa
    14341 cgcagatgaa caaggcaatg ccgtctgaag ggctttcaga cggcattttg cgctattttg
    14401 tgaaggtagg gatgacggtt cgggtattcc tgacagggtg gattttcaag gtgttgtata
    14461 gggtgtagga ggattcgtaa aaggtgggat gcagggtgtg cttcagcccg ctgcatcaaa
    14521 aatttttgga gaatcggcgg gagtcggcgg ttttggtttc ggcggggacg gtggaaatgg
    14581 gtaacattga cggaatcgac ggaagcggtg ggcttcagcc caccgcttgt atattggaat
    14641 caccgtatca tagcgccacc cgcgcccacc caaggcacac aaccgttgcg tagctcaggg
    14701 agcggcaggg caacccatcg acacaaccgg acagttgccg gacaacacaa ccgaatgcaa
    14761 ggcaggttta tgatgagtac ccaataccat tacgcaggta tagtgaatta aatctaaacc
    14821 agtacagcgt tggttcgcct tagctcaaag agaacgattc tctaaggtgc tgaagcacca
    14881 agtga
//
LOCUS       DAWKOG010000035        14856 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.035, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000035 DAWKOG010000000
VERSION     DAWKOG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14856
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.035"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(120..815)
                     /locus_tag="WJK66_000812"
     CDS             complement(120..815)
                     /locus_tag="WJK66_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ7578555.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSAQSSSSAPAAPAE
                     LNEGVNYTVLSTPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
     gene            complement(986..1645)
                     /locus_tag="WJK66_000813"
     CDS             complement(986..1645)
                     /locus_tag="WJK66_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEZ7578556.1"
                     /translation="MKTAILIDGAFFIKRVRSLGLSQNPHDAKRIADLAFRYSMLHLK
                     DKINGTDQMDDLYRIFFYDCVPFEKKMHNPVSHRAIDFSKTPEAIFRCNLHTELKKKR
                     KLALRLGKLSDNTAWILKPQVMKNLLKNPSTQITENDVVLDVKQKGVDMRIGLDISSI
                     TLKKQADKIILFSGDSDFVPAAKLARREGIDFILDPMWNHVPPDLMEHIDGLKTKCPK
                     P"
     gene            complement(1843..3955)
                     /locus_tag="WJK66_000814"
                     /pseudo
     CDS             complement(1843..3955)
                     /locus_tag="WJK66_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(4141..4746)
                     /locus_tag="WJK66_000815"
     CDS             complement(4141..4746)
                     /locus_tag="WJK66_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ7578557.1"
                     /translation="MTRQSLQQAAESRRSIYSLNKNLPVGKDEVVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAAVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            complement(4795..5193)
                     /locus_tag="WJK66_000816"
     CDS             complement(4795..5193)
                     /locus_tag="WJK66_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ7578558.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            5301..6212
                     /locus_tag="WJK66_000817"
     CDS             5301..6212
                     /locus_tag="WJK66_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ7578559.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTGAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVALELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGSLVRLLPEYTVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            6202..7503
                     /locus_tag="WJK66_000818"
     CDS             6202..7503
                     /locus_tag="WJK66_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEZ7578560.1"
                     /translation="MPSETARPLYADTCAAHTLVWFRQNLRIRDNAALCAAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELNKHGIALTFVNDRAVFGKTDLIPDGGTAHTDFDRYRE
                     VWLDRFSKQPPAGPDLFAAYRQPFPENLSAPPPAALSDGIFLPQNSGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKHTSLMGAYLSAGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHTDDDPSDGNPEHTLRLTLWQQGRTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWRLAASRHTCPDIAAAAHKTDPDGT
                     FVRRHIPELAHLSADTVHTPWRFACSVDTHGYPAHPVAGIP"
     gene            7566..7862
                     /locus_tag="WJK66_000819"
     CDS             7566..7862
                     /locus_tag="WJK66_000819"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578561.1"
                     /translation="MTQYLPFFSLSIIRYAQITATQAATVPIVGKKCCQSVIGGTGQR
                     PHSISAPPNNRYSSISAIQSNNGYFKRCNQKTNHVRTIYLPPFRQRYCTASVQC"
     gene            7896..10046
                     /gene="dinG"
                     /locus_tag="WJK66_000820"
     CDS             7896..10046
                     /gene="dinG"
                     /locus_tag="WJK66_000820"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HEZ7578562.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LTREEGEEAPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELTFALAKGRGRYLCPYKLYQLTQNNAQQNLLGFEAPEVLWD
                     SKPKPEELNLLRDIADEFSARRFNGDRDTWPEKIDDAIWLKVTNDRHGCLKAACPNRA
                     ECPFYLARDMLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAASSLLDSLHEWQFHLAE
                     EPSLSLGPSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVIGLNDALS
                     AARREKEQDSALIDRLTGEFGLFISRIEQISAVWDLLSTVPIEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLMLRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAIIEWLPKLISPGEAIGTLVLFSSRK
                     QMQDVALQLPDDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRVKTQRYGQQLLAGLPPFKRIG"
     gene            10082..10696
                     /locus_tag="WJK66_000821"
     CDS             10082..10696
                     /locus_tag="WJK66_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEZ7578563.1"
                     /translation="MNFTRLLNQVLSTVQKKGNTFSDSPLNSFGGGALVAGVASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGETEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIEDIAAAVGNDEALAA
                     EAYLAARLVCADLSRKETVFLARLSQALELDDNLVENLERQLGF"
     gene            complement(10791..12659)
                     /locus_tag="WJK66_000822"
     CDS             complement(10791..12659)
                     /locus_tag="WJK66_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ7578564.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVMIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVELSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLRNISIILFLILTATSFLPSGFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTANGKRQLLSSLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFPARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLIKDIPNV
                     HWVDAQKYLPKNTVEIYGRYLYGDQDHLTYFGSYYMGREFHKHERLLKSSRDGALQ"
     gene            complement(12740..14110)
                     /gene="purB"
                     /locus_tag="WJK66_000823"
     CDS             complement(12740..14110)
                     /gene="purB"
                     /locus_tag="WJK66_000823"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEZ7578565.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIAEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKSLQAQEFLGKINGAVGNYNAHMV
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLAEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKGFIGLLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            complement(14182..14814)
                     /locus_tag="WJK66_000824"
     CDS             complement(14182..14814)
                     /locus_tag="WJK66_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEZ7578566.1"
                     /translation="MENTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFSCRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAASLFAISDDEDEIVAYLSEHGLQTA"
BASE COUNT         3543 a         4111 c         3828 g         3374 t
ORIGIN      
        1 ctatatattc tgatttaaaa ccataaggct ttaagcaatc atctcttcta taaagcctaa
       61 atacaaaagg ccgtctgaaa tcctattttc agatggcctt tactcattca atcctcaact
      121 tattgcggct ttttctgctc ttcgcgtact ttatccacca actggtcaat cgtggtcata
      181 ccggactgcc agtctttaaa ttcaacttgg tatttgccgc cgacaatcac agtcggtgtg
      241 ccgctgattt ggaatttatt ggtcaactct tccatttgag ccgcacgcgc ttggctttca
      301 ggagcctcaa atgcagccaa tacttttttg ccgtcaaacg ctgtttgctc ggacagccat
      361 tttttcaggg tatcggtatc ggccagattg attttttgat taaccatcgc atcgaaaata
      421 tggctgttgg ctttatctga ttcaccggcc atttccactg cggccgccaa acgtgccaaa
      481 ggtttcattt catcacccca cacgacatgc tcccggcgca tataggtatc gtctttaaac
      541 gttttgatgt gctcgctcaa gaccggctca agatgggcgc aatgcgggca gaagtagccg
      601 aaaaattcca atacttcgat tttaccggcc tgctgttgcg gaataggcgt agacaataca
      661 gtgtagttca caccttcgtt caactcagca ggggctgccg gagcagatga gctgctttgg
      721 gcgctgtctg ccggaacact ggtttcagcc tgtttgctac aagcggccaa tgccaacagg
      781 gtcaatgaag tcaaagctaa ggttttcagt ttcataggta tctcttgtgt gtcagattaa
      841 tgtgcggatt ttatggcatt ttattgaagg cgtgttcgat ttgagtgaaa aaatgtgttt
      901 agttttattt cagcctttat aaaggccgtc tgaaatatgg cttagataat tagccttatt
      961 ttcagacggc ctttgttcta gtaatttacg gtttcggaca ttttgtcttc aatccgtcga
     1021 tgtgttccat taagtcaggt gggacatgat tccacatagg atcaagaata aaatcgatac
     1081 cttcccgtct ggctaatttg gctgctggga caaaatcgga atcaccagaa aacaagatga
     1141 ttttatcggc ttgttttttt aaggtaatag atgaaatatc caagcctata cgcatatcta
     1201 cacctttttg tttaacatcg agcacgacat cgttttccgt aatttgagtt gacgggtttt
     1261 tcagaagatt tttcatgact tggggtttta atatccatgc tgtattgtcc gacagcttgc
     1321 ctaaacgtaa cgctaattta cgcttcttct tcaattcggt atgcagattg caacgaaata
     1381 tggcttccgg agtctttgaa aaatctatgg cacgatgaga tacgggattg tgcattttct
     1441 tttcaaacgg cacgcagtcg taaaagaaaa ttctatataa atcatccatt tgatctgtgc
     1501 cgttgatttt atctttcaga tgcagcatcg aatatcggaa agccaaatca gcaattcttt
     1561 ttgcatcgtg tggattttga gacaggccta atgaccgtac ccgcttaata aaaaatgcac
     1621 cgtcaatcaa aatggcggtt ttcatattgc ttcccctata tttgtcaaag atataaaaaa
     1681 gcccttggga tcggcatacc cgctatgatt taatgggtaa tgcgtactgc caagggcact
     1741 atttcttttt ggattatact gatgtagata aagcctgtca aaaagttcgt atgctgaatt
     1801 tacggattat caggattttc agacggcctt tgcctgattt catcagaaac ggtaatttac
     1861 ccgggcagta tagccgcgcg gattacccgg catagagtcc gaacgccaat atttttgatt
     1921 gaacagattg gctgcggcaa aggtaacgtt aacattttta tggttccagc caagcatggc
     1981 atcaactcgg gcaaagcctg gaagcgtagt cacttcttta tttcttgagt cgtaaccgta
     2041 gcgtttgcct gtaccggtta cgccgatttc gccgtagagg ttttcggtcg gggtataacg
     2101 gaaaaacagg ttgccggtaa cgttgctggt gttattcaaa tggatgccca ctcggtcggg
     2161 attttctttg tcttcaacga ctttcgcctg catcacgccc aacgaaccgc gcagatagag
     2221 tttttttggg gatgatttgc ccgatggcgg acaattccac gccgcgcgaa cggtgtttgc
     2281 cgctaaccgc ataaatataa gggttgtttt ttggatcggg gcggtagcgg atattgaagc
     2341 gttcgatttg gtaggcagac aacgtagtgc tgaggcggtc gtccagccaa ctgcttttca
     2401 cgccggtttc gtattggcgg gtgtactcgg ggtcggcgtt gaacacggcg gaagacaacg
     2461 tatcgatgct caaatagccg ccgcgtccgc cataaggcgc gaagcctttg ttatacgagg
     2521 cgtaaagtgt gtggacggga ttgatgttcc acactgcgcc gatgttgggg ctgaacgagt
     2581 gtccgctgta ttggcggctg ctgccggtga gtttgttttc ggaattaaag gtgtatttgt
     2641 cgtaacggcc gccgaggacg aatttcaaat cgggcgtggc ggagaagatg ttttgcacaa
     2701 agatgccgta ggagtcggct ttgtggcggt tttgggtcag aataggctgc aatctgcccg
     2761 aagccggcca gcttgcgcgg tcgtaggggt tgatggaggc ggaaaaggcg ctgctgaaac
     2821 ccaatgtcgg gttgcggtgt tcgcggctgt aatccatgcc tacggtcagg tggttttcaa
     2881 aacggccgat ggtgtagtcg ccgttgagcg ttaagttgga cgacagggtt ttgttgtcgg
     2941 tctgctgcca ggcgtagtta cgtttgatta agttgccatt ttcgctgcct gcatagaaat
     3001 gatcaaaatc ctgcgccgcc gtgcggtggg cgagctgcca ttgggcacgc catttgtcgt
     3061 tgaaggcgta ttcaaggtcg gaacgccaaa cttgcagctt gtctttgaca aaatcgttcc
     3121 ggtgggcgaa ccccatgcgg taaggcagtc cgaagcggtc gtacacggac ttggtcggac
     3181 tgcggtcggg cgtgcgctcc acattgtcgt aggtgtattg ccccgtccac ttcaagccgt
     3241 tgtcgagttt gacggtaatg ctgggcgaaa ccatgacatt tttgctgtct atgccgctgc
     3301 ggaacgaatt ggcgcgcccg acttcgccgg tgagacggat ggcgacgttt ttgttcagca
     3361 cttcgttgat gtccatattc aggctacggt ttgcccacga accataaacc gtaccgatat
     3421 tacggctttg tttgaagttg gcgtatttgc tgaccatgtt gatgacaccg ccgccgttgg
     3481 tacgcccata aagcacggag gacggacctt tcaggatttc cacgcgctcg atgttggcgg
     3541 tgctacggcg cacctgcccg ctttcgcgta cgccgtcgcg gtaaatatca gatgcgtcgg
     3601 cttgaaagcc gcgcaggaaa atgctttcgc cgcgcatatc gtaggcggcg tcgatgccgg
     3661 cattgccttc gaggatggaa ctcaaatcgt tcgtgccgta atttttgttt ttctggatat
     3721 tgagcgtatc gatggtttgc ggcgtttctt tgatgagctg tccgttgcgg gtaacggcgg
     3781 cttcgtcgta gttgatgtag cctttgagta cgctggtgtc ggactgtccg accacggaaa
     3841 cggtaggtag cgtggcggtg taatgttcac cattgtcctg cgtatcggcg gcagcaacag
     3901 ggaaggaagc aataatcagc gtgggtaata aagccaaatg aaatggtatt tgcattttta
     3961 tactcaattt aacaaaacaa ccgaattata ttgcctcacg gaggaaatga gaataatttc
     4021 ttttaactat attgaacatg atatttgtaa acaaaggtct cagaatgcgg aaaactcgcc
     4081 gcctgatact gaaaaatgcc gtctgaacag ggttcagacg gcattttttt gaccgcgaaa
     4141 ttatgcgccg aacactttca aacgttctgc aactggttca aaggtctttt cacctgccgc
     4201 cccttcaata ccgccgataa ccatttgtgc gcgcaacaac cagttttcgg ggatattcca
     4261 cgctttggca atcgccgcat cgggcaaggg attgtaatgt tgcaggtttg cacctacgcc
     4321 gaccgcggca agtgtcgtcc aaacggcata ctgcaccatc gcgttcgcct ggtccgccca
     4381 aacgggaaag ttggcggcat aagcagggaa ctgctcctgc aaacctttga cgacattttg
     4441 atcttcataa aacaaaatgg ttgccgcacc cgccttaaac aggttcaatt tttgcgcggt
     4501 cggttcaaaa ctgtcggcag gcacggcggc acgcagcgcg tcttcgacaa attgccacac
     4561 cttatcatgc tcttcgccaa acagcacgac cacgcgggca gattgggaat tgaacgaaga
     4621 aggtgtgtgc aaaacggcgt gttcgacgat ttggacaact tcatctttgc cgacgggcag
     4681 atttttattt aacgaataaa tggaacggcg gctttcggca gcctgttgca gagattgacg
     4741 ggtcatttct tttcctcaaa aacattcaac aaaatacact ttaggcagtc cgattcaggc
     4801 tttacgcttg aaaaacagcc tgtccagcga ccatgctccg ccgcccgccg ccgcgatata
     4861 gaggaatacg aagcagaaca gcactgcgga ctcgccgccg ttggcaatcg ggaacaaagc
     4921 atttccggaa gcgtgcgcca taaaataggc aaccgccatc tggccggaca aaacaaacgc
     4981 ggcagggcgc gcaaacaggc ccaacaccag caaaatgccg ccgacaattt ctaaaatacc
     5041 ggcaagcagc aacagcccgc cgggcgaacc gctgcccatt tcaatgggga aggcgaagat
     5101 tttcgacgta ccgtgcaaca aaaacaggta ggcggttacg atacgcaaaa cagaaagcaa
     5161 aaccggttgg atacggttgc agcaatcgga cataaaagct cctttgatat ttgtcttcat
     5221 ttcaggcggt agagtataga tgtttcccct tttcaaatat atactgcttt tattgataca
     5281 acttttccac acaagaaaat atggacaccc tgttcagcct caaggttttc cgccaagtcg
     5341 tccaaagcgg cggcttcacc cgcgccgccg acgcgctcgg catctccacc gcaatggcaa
     5401 gcaaacacgt cagccacttg gaaaacaccg tccaagccaa actcctgcac cgcaacagcc
     5461 gcaacctcag cctgaccgga gccggggaag aatactaccg gcaatgcagt tacgcgctcg
     5521 acacgctcga cgatgccgcg caaaaagccg ccggagggac ggaaaaaccg caggggctgc
     5581 tgcgcgtaac gatgccgctg tggtttgccg gcagccagat atgcaactgg ctggcggaat
     5641 accgcgaacg ttatcccgaa gtggcattag aactgatttt ggacaaccgc cacgtcgatt
     5701 tgattgccga aggcgtggat ttggcgttgc gcgtttccca aaccctgtcc ccgtcgctga
     5761 ttgcgcgccc actggcggaa atcgaatttg ccctgctcgc ctcgcccgat ttcctgaggc
     5821 gcaacggcgt gccggaaacg ccggaagaag tggcggggct gcccgccgtc ctgccgacct
     5881 acaccaacca gcagaaactc gacctcaccc gcaaatcgga cggcaaaaaa taccggcttg
     5941 aactgacccc cgtcatccgt accgacaaca cgctgatgat gcgcgaaatg attaaggcgg
     6001 gcgcgtgcat cggttatcag ccgctttggg cggcggaaca cgatttgcgc tgcggctcgc
     6061 tggtgaggct gctgcccgaa tataccgtcc cgaccgaccg cctgaatgcc gtttatgcgg
     6121 acagggcgtt cttaagcgcg aaagtccgca gcttcatcga ttttctgaac gaaaaaatcg
     6181 ccagcaggaa aggctgccga aatgccgtct gaaaccgccc gcccccttta tgcggacacg
     6241 tgcgccgcac acacgctcgt ctggttccgc caaaacctcc gcatccgcga caacgccgcc
     6301 ttatgcgccg ccgttgccga aggtttgccc gttatcggta tttggattga cgatgccgaa
     6361 acagacaacc ctcgccgcgc cgcgttctac cgccaatccg ccgccgaact cgcccaaggg
     6421 cttgcagggc gcggcatccc gctctacacg gcggcatcgc ctgccgagct cgtccggctc
     6481 gccgtccgcc tcaatatccg cgccgtcatc gccgacgaat cccatacttt tgccgacaaa
     6541 ctcgccgaca acgccctttg gcatgaattg aacaaacacg gcatcgcgtt aaccttcgtc
     6601 aatgaccgcg ccgttttcgg caaaaccgac ctgatacccg acggcggcac ggcacacacc
     6661 gatttcgacc gctaccgcga agtatggctc gaccgctttt ccaagcagcc ccccgccggt
     6721 ccggacctat tcgcggcata ccgccaaccc ttccccgaaa acctttccgc cccgccgcct
     6781 gccgcccttt cagacggcat cttcctgccg caaaacagcg gcgaaacggc ggcttggcgg
     6841 cagtggcggc ggtttctcga acaggcggat tcctactccg ttttaaagga tttcccctcg
     6901 cgcaaacaca cttcgctgat gggcgcgtat ttgagtgccg gctgcatctc gccgcgcctg
     6961 ctcgcgcggg aaagcctcga acgccgtctg aacgcgtggg cggacaacat catccgccgc
     7021 gattttttcc ttcaacttgc cttgcagcac acggatgacg acccttcaga cggcaatcct
     7081 gaacacaccc tgcgcctgac gctttggcag cagggccgga ccggcattcc gattatcgat
     7141 gccgcgatgc gctgtttgca caaaaccggc agcctccacc ccgccctgag acgcttgagc
     7201 gcggattttt tctgccacgt tttaaacctc ccccgccgcg aaggcgagat atggtttgcc
     7261 cgacagctga ccgatttcga tgcagcaatc aaccaaggca actggcggct tgccgcctca
     7321 cggcacacct gccccgacat tgccgccgcc gcacacaaga ccgaccccga cggcaccttt
     7381 gtcagacggc acattcccga gcttgcccac ctgtccgccg acaccgtcca cacgccttgg
     7441 cggtttgcct gttcggtcga tacccacggc tatcccgccc atcctgtcgc cggtattccc
     7501 taagcggtac ggcaacctaa aacaaatgcc gtctgaactt ccgtttcaga cggcattgcg
     7561 tccgaatgac tcaatatctt ccgttttttt cactttcaat catcaggtat gcccaaatca
     7621 ccgccaccca agccgccacc gttcccatcg tcggcaaaaa atgctgccaa tccgtcatcg
     7681 gcggtacagg gcaacggccg cattcgattt ccgcgccgcc caacaaccgg tacagcagca
     7741 tatccgcaat acaaagcaac aacgggtatt tcaaacgctg caaccaaaaa accaaccacg
     7801 tccgcacgat ttacctcccc ccattccgac aacgatattg taccgcttcc gtgcaatgtt
     7861 aaaataaccc cacttctacc gccgtacaaa acgccatgct caccgattta gaaaaaaacg
     7921 ccatccgcga ccattaccaa aacatcggca aaaacctgcc cggtttccgt ccgcgtgctt
     7981 cgcagcggga aatgattgcg gcggttgcca acgctttttc gcggacgttg acgcgtgaag
     8041 aaggcgaaga agcacccaaa cgtgagggcg agagcattgc cgtgatcgaa ggcccgaccg
     8101 gcgtgggcaa atcgttggcc taccttttgg ccggcggcat catggcgcaa acgcgcggca
     8161 aacggctgat tgtcagcagc gcgacggtgg ctttgcagga gcagttggtt gaccgagatt
     8221 tgccgtttct ggtcgaaaaa agcggtttgg agctgacctt tgcgcttgcc aaagggcgcg
     8281 gccgttatct ttgtccgtac aaactctatc aactgacgca aaacaacgcc cagcaaaacc
     8341 tgcttggttt tgaagcgccg gaagtgttgt gggacagcaa acccaagccc gaagaattga
     8401 acctgctgcg cgacatcgcc gacgaatttt ccgcccgacg gttcaacggc gaccgcgaca
     8461 cctggccgga aaaaatcgat gacgcgattt ggctcaaagt taccaacgac cgccacggct
     8521 gcctgaaagc cgcctgtccc aaccgcgccg aatgcccgtt ttacctggca cgcgatatgt
     8581 tggaaaccgt cgatgtcgtc gttgccaacc acgatcttct gcttgccgac atcagtatgg
     8641 gcggcggcgt gattctgcct gcgcccgaaa acagtttcta ttgcatagac gaagcgcacc
     8701 acctgcccaa aaaagccctc agccgttttg ccgccgaaca ttcatggaat attgccgttt
     8761 ggacgctgga aaaactgccg caactgaccg gcaagattgc cgcgctgacc gataaagccg
     8821 aacttgccaa ccttgccgac gaagccgcct catccttact cgacagcctg cacgaatggc
     8881 agttccattt ggcggaagag ccgtctttga gcttggggcc gtctgaaaac gacaggcgta
     8941 ccaacagcga accgacttgg ctgtgggaag acggcaaaat ccccgaaggc ctcgaaacca
     9001 ccgtttccaa caccgccatt gccgcacgca gcctgctcaa acacgttatc gggctgaacg
     9061 atgcgctttc cgccgcgcgc cgcgaaaaag aacaagacag cgcgctcatc gaccgcctga
     9121 ccggcgagtt cggccttttt atctcccgta tcgaacaaat tagtgcggtt tgggatttgc
     9181 tctccaccgt tcccattgaa ggcgaagagc cgttggcgaa atggataacc cgccgtgccg
     9241 acgataaaaa cgactatatt ttcaacgcca gccccatcag cagcgcgtcc caccttgcca
     9301 acagcctgtg gcggcgggcg gcaggcgcag tgttgacctc cgccaccctg caatccttgg
     9361 gcaactttaa cctgatgctg cgccaaaccg ggctgcaatg gctgcctgaa accaccaccc
     9421 tcgccctcaa aagccccttt gactttgaaa aacagggtga actctacatc cctcccatat
     9481 acgccagccc caaagacccc gaagcccaca ccgccgccat catcgagtgg ctgcccaagc
     9541 tcatttcgcc cggcgaagcc atcggcacgc tcgtcctgtt ttcctcgcgc aaacaaatgc
     9601 aggatgtcgc cttacaactg cctgacgact atctgccgct cttgctcgta caaggcgaat
     9661 tgcccaaagc cgttctcctg caaaaacacc accgggccat agaagaaggc aaagccagca
     9721 tcatcttcgg actcgacagc tttgccgaag gactcgacct gcccggcacc gcctgcgtgc
     9781 aagtcatcat cgccaaactt cccttcgcca tgcccgacaa ccccatcgaa aaaacccaaa
     9841 accgctggat agaacagcgc ggcggcaacc ctttcatcga aatcaccgtc cccgaagccg
     9901 gcatcaaact catccaggcc gtcggccgcc tcatccgcac cgaacaagac tacggccgcg
     9961 taaccatcct cgacaaccgc gtcaaaacac agcggtacgg ccaacaatta ttagccggcc
    10021 tgccaccgtt taaaaggata gggtaaacct accgccctgc ccgcaacaga aaggaaaaat
    10081 catgaacttc acccgactgc tcaaccaagt cttaagcacg gttcaaaaaa aaggcaacac
    10141 cttctccgac agcccgctca attcattcgg cggaggcgcg ctggttgccg gtgtcgcctc
    10201 catgctgctg aacggtaaaa accgcaaaac catcaccaaa atcggttcga ccgccgcttt
    10261 gggctacctc gcctaccggg gctatcagat gtggcagcaa aacaaagggc gggcaaccgt
    10321 aacacaaagc gatttccaac ctgccggaga aaccgaagaa acatacagcc gcaccgtatt
    10381 gcgcaccatg atagccgccg ccgcttcaga cggcatgata gacgaagccg aacgccggac
    10441 tatcgaacag gaaagcggca cagaccccga aactgctgca tggctcgccg ccgaataccg
    10501 cctgcccgca agcatcgaag acatcgccgc cgccgtcggc aacgatgagg cgttggcggc
    10561 ggaggcctat ctggcggcaa gactggtatg tgccgatttg tcgcggaaag aaaccgtctt
    10621 cctcgcccgc ctgtcgcagg ctttggaact ggatgacaat ctggtggaaa acttggaaag
    10681 gcaattgggg ttttgattca aaattccctc ccgcagggga aggaaaataa aaaccgccgc
    10741 cgccccgaaa ggcaaacggc ggcacaaacg atatcggacg gcaaggcagg ctactgcaat
    10801 gcgccgtcgc gagaagattt aagcaggcgt tcgtgtttgt gaaattcccg ccccatataa
    10861 taagaaccga aataggtcag gtggtcttgg tcgccgtaaa gatagcggcc gtatatttcg
    10921 accgtgtttt tgggcaggta tttttgtgcg tccacccaat gcacattggg aatatcttta
    10981 atcaaatcaa agaccgcctg attgctcttg ccgatgtcgc ccatagcctg aatggggcgg
    11041 agatattggt ttgcggcaaa tcttttcaat ttttcctccc tcaggggcga acggctgatt
    11101 gatgtgttgt ttgcaaaaac atagacgggt ttgacggcgg ctatcctttt gacggtttcc
    11161 ctgaatcggg ctgggaaccc gggtattagg aaggattgcg cttcaaatct cggcacgggc
    11221 tggccgccca tcctcaaatc atagaattgg gcaatgaaaa cggcttcggc tttttcaact
    11281 tcatcccggt attttcgaca taacgggttg tctgccagct tctcatctac ccaaaccaaa
    11341 cactccgaat cgagggacag gattttggct ttccaccctt cccggctgcc gacataatcc
    11401 agaaaccccc gcaggtgtcc ggcgtgcgag tcgccgaggg tcaggacggt ttccggaaaa
    11461 tgattttccg cagcaagggg cgcgccgggc aacgggcgga ggtgttcctg tttcaatatc
    11521 ccccttgcgt acaggttgta accgacaagt atcagggacg gggcgagata gaggcagaaa
    11581 aatgcctttt tgaaggtcat cttccgtttt ctaagcggct gttcaatcaa ataataactc
    11641 aacagggaaa atccggccgt caacgcggca accgccgata cggcaggcag tccgagctgt
    11701 ttgtcgcctg taatgtaatg ggcgaaagca ataaaaatcc aatggtacag gtatagggaa
    11761 taagagattt tgccgacaaa tacgatgggg cttgccgaca ggatgcgggt cggaagtgtc
    11821 ccgtattgca tactccggat aagcagtgcc gtcagcaggc agggaaggag cagggtcatt
    11881 cccgggataa acggattgtg tttgtcaatc acgaacaggc aggcaagcaa tgcgccgaag
    11941 cagagtgatg aaagcaactg ccgttttcca tttgctgttt gccgtctgcc gttttgcgtt
    12001 tgcccgtaaa ccgccagcag cgaacctgcc aacagctcgg gaaacctcag tgtcgaaagg
    12061 taataagtat tgggttggtt gagaatatcg gtataaaacc cgcttggcaa aaacgatgtg
    12121 gcagtcaaaa tcagaaatag gatgatgctg atgttacgca gcacccgtag cgattttgtt
    12181 tttttgcagc aaaatatcag caaaagagga tacaggaggt aatactgttc ctctactgcc
    12241 aaagaccaga tatgcagtac ggggttctcg tcggcactca aatcgaaata cccctgctga
    12301 aaccccagat aaatattgga caagaaaacc gcagaaagct ccacggtttt ccgcatttgg
    12361 ttgaaatctt cgtaaaggaa gatttgagag gcaatcaccg aagccagcga cacggccgca
    12421 ataaaagcag gataaatccg cttaatcctg cgggtataaa aatcccggaa agaaaaagaa
    12481 ccgttctgta tttcagaaag aatgatgccg gtaatgagga atcctgagat gacaaagaaa
    12541 atgtccaccc ccaggaatcc tccgggcagc cagcggttat tcaggtggaa aatcatgacg
    12601 gatagcacgg cgacggcccg caatccgtca atttccggtc tgtatcggac agcttgcata
    12661 aatatcgccc ctgtatgtcc cgttatctta aaaatgccgt ctgaacgcgc gttcagacgg
    12721 catcggtttc agtaaacgct caaatccgtt tcgccaacgc ttcagccttg cccacataca
    12781 gcgcgggggt cagctcaagc aatttggctt tggcttcggc ggggatttcc agcaatccga
    12841 taaagccttt cagcacttcg ggcgtgatgc cgcctttgcc gcgcgtcagg tctttcagct
    12901 tctcgtaagg attggccacg ccgtaacggc gcattacggt ttgaatcggc tcggcgagca
    12961 gctcccaagt ggcatccaaa tcggcggcaa gcgcggcggg gttaggttcg agcttgttca
    13021 gaccgcgcag gtgggcggcg aaacccaaca cggcatagcc cacgcccacg cccatattgc
    13081 gtaagacggt gctgtcggtc aggtcgcgct gccaacggga aatcggcagt ttttccgcca
    13141 aaaaacccaa tacggcgttt gccataccaa ggttgccctc ggagttttca aagtcgatgg
    13201 ggttgacttt gtgcggcatg gtggaagaac caacttcgcc cgctttgact ttttgtttga
    13261 agtaccccaa agaaatgtag ccccaaacgt cgcggttaaa gtcgatcagg atggtgttga
    13321 tgcggctgag ggtttggaag aattcagcca tgtagtcgtg cggctcgatt tggatggtgt
    13381 aggggttgaa ggtcaggcca aggctgattt cgacgaagtt gcggcagtgg gtttcccaat
    13441 cgacgtcggg ataggcgacc atgtgggcgt tgtagttgcc gaccgcgccg ttgattttgc
    13501 cgaggaactc ttgcgcctga aggcttttaa actggcgttg caggcggtac acgacattgg
    13561 cggtttcttt gcccaaagtg gtcggcgtgg cgggctggcc gtgggtgcgg ctcatcatcg
    13621 ggacggcggc aaggtcgtgc gccatagcgg tcagtttttc gatgatttct gccagtttgg
    13681 gcagcaaaac agcctcacgc gcttcttgca gcattaaagc gtgggacagg ttgttgatgt
    13741 cttcgctggt gcaggcgaag tggatgaact cactcacggc ggcgacttcc ggcacttcgg
    13801 caaaacgttt tttcagccaa tattcaatgg ctttgacatc atgattggtc gtggcttcga
    13861 tggctttgac ggcggccgcg tcttccaatg aaaagttttc aatcaccgtg tcgatttcgg
    13921 caagcgtttc ggcactgaag ggcggcactt cggcaatctt cggctcggcg gcgagggctt
    13981 tgagccagtt taattcgact ttgacgcgcg ctttcatcag gccgtactct gagaaaatcg
    14041 ggcgcaatgc ttcaacggat tgggcataac ggccgtctaa aggggaaagc gaggcgatgg
    14101 ggttgatcat ttcggcatcc tgttcgggaa agacatcaaa aattgttaaa ttgtttgcaa
    14161 ttatacatca ctctcaggac gctatgccgt ctgaagcccg tgttccgaca gatacgcaac
    14221 gatttcgtct tcatcgtccg atatggcgaa caaagaggct gccccttcgg aaatcagacc
    14281 gcgcgccaaa agctgcgcgt ttatccactc cgccaagccc gaccaaaacg cctttccgac
    14341 caaaacaatc ggacgcggcg gcactttgcc cgtctgcacc aaggtcagga tttcaaacaa
    14401 ttcgtccagc gtcccgaagc cgcccggcat cacgacatat gcttgggaat agcggaaaaa
    14461 caccgccttg cgttcggcaa aacgggaaaa ccgcaaggcg atgtcctgat acggattcgg
    14521 tttctgctcg tgcggcaaaa cgatgttcag ccccaccgaa accgacttcc cggcaaacgc
    14581 gcccttgttt gccgcctcca taatccccgg cccgccgccc gaaatgacgg caatgcccga
    14641 atccgacagc cgccgcgcca gacggcagga gaacgcataa tccgcatgat tctgcggcgt
    14701 gcgcgcgctg ccgaagatac tgactgccgg aaacacgccc gccaatgctt cgtctgcctg
    14761 cctgcgttcg gcatcataac gtgcctgctc cggcacacgg tttgtattct ccattccatc
    14821 ctccgttcaa aaacagcgat tgtacaccgt caaaaa
//
LOCUS       DAWKOG010000036        14576 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.036, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000036 DAWKOG010000000
VERSION     DAWKOG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14576
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.036"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..382
                     /locus_tag="WJK66_000825"
     CDS             <1..382
                     /locus_tag="WJK66_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7578567.1"
                     /translation="LERLAARDIIRFPGFVNNVSEVIKEHHIFVLPSYYREGVPRSTQ
                     EAMAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILAEKMIYFIENREAVRLMGNA
                     SYAIAKDKFDAEKVDLKLLDILKA"
     gene            complement(625..1476)
                     /locus_tag="WJK66_000826"
     CDS             complement(625..1476)
                     /locus_tag="WJK66_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEZ7578568.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVENWLSDNP
                     LLERKDTDEFSDAEFSHYTAPARQIGGDEGEDMLSNIAGEQDFKQYLHAQVCEHPLSD
                     QEAACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLQHALTALKKFDPAGVAA
                     ADLNESLILQIERLGECAAKPAALHIVRNALDSIDGNRSQTLARIKKHLPQTDSGTLE
                     AALDLIASLNPFPAAGFASSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESLEIPAA
                     HKRKTAE"
     gene            1825..3339
                     /gene="katA"
                     /locus_tag="WJK66_000827"
     CDS             1825..3339
                     /gene="katA"
                     /locus_tag="WJK66_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HEZ7578569.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGVGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQPGLL"
     gene            4328..4771
                     /locus_tag="WJK66_000828"
     CDS             4328..4771
                     /locus_tag="WJK66_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ7578570.1"
                     /translation="MKAIQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQMSEAL
                     TLAEGQKTAVVEYYSDNGVFPKNNASAGVASASEIVGKYVAKVDVSGNTITSTMKTSG
                     VNKDISGATLVLEGAQKSGSFTWTCKAGSSNGVKDKFLPSSCRAK"
     gene            5033..5533
                     /locus_tag="WJK66_000829"
     CDS             5033..5533
                     /locus_tag="WJK66_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ7578571.1"
                     /translation="MSNYRRDFTRGAHYFFTLALQDRTKDYLIRHIDRLRLAYRETKT
                     RYPFETAAICILPDHLHFIMRLPEGDHDYPIRIAYLKTRFSQLIPKECRAPNGSKTKK
                     REAGIWQRRFWEHLIRDEQDLNNHRDYIYFNPVKHGYAASVKEWRYSSFFKDVEKGIY
                     PLDWGG"
     gene            6009..6575
                     /locus_tag="WJK66_000830"
     CDS             6009..6575
                     /locus_tag="WJK66_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HEZ7578572.1"
                     /translation="MSLYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGT
                     VEALTPLAGWVGGFYAKPFISEWDHWAAFVLLGGLGLKMMHEGLSGEAEDARENKRES
                     WWLTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAIIGMATTVLVAVGLTVGRALGVLF
                     GRRAEFAGGLVLIAIGTWTLLSHLGLIG"
     gene            complement(6851..8887)
                     /locus_tag="WJK66_000831"
     CDS             complement(6851..8887)
                     /locus_tag="WJK66_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEZ7578573.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTGWANTVEPLTGITERVGRIWGVVSHLNSVADTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSESKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELSDDGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLGLADLQPWDLG
                     YASEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGKTIGGVYMDLYAREGKRGGAWMNDYKGRRRFSDGTLQLPTAYLVCNFAPPVG
                     GREARLSHDEILILFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YNVLAQMSAHEETGVPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKKVAVIQPPEYNRFTLSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHSGFDNAA"
     gene            complement(8975..9847)
                     /locus_tag="WJK66_000832"
     CDS             complement(8975..9847)
                     /locus_tag="WJK66_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ7578574.1"
                     /translation="MEIWNRLDTWLGAVPIRAEAVESVAAVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNYPAGSETL"
     gene            10117..12507
                     /gene="gyrB"
                     /locus_tag="WJK66_000833"
     CDS             10117..12507
                     /gene="gyrB"
                     /locus_tag="WJK66_000833"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEZ7578575.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLNV
                     VGDSDKKGTTVRFLASTETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPAEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIVSDSRTIEGEELANTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEVALERIEGHEGHRFIKITRKLHGNVVVSYLEPKFLNSKAYQTL
                     TQTAAALKGMVGKGAKLYKGDNEYDIDSFEGALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            12606..13991
                     /locus_tag="WJK66_000834"
     CDS             12606..13991
                     /locus_tag="WJK66_000834"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEZ7578576.1"
                     /translation="MVSIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQAVRIVIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLVGIPERLERIRTQHILRLNGQEIRFIP
                     DRDLNILGNQVLPKHPNGLRFTAYASDGTVLNEQVYYSVGGGFVVTEEDFDRQAETEK
                     AVPYPYTSCAELLARCRLNRLDISEVVLANEAALAGCGEAEIRRRAAAVAEVMEGCIK
                     RGLGADGELPGGLNVRRRAPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTAPTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
     gene            complement(14062..14160)
                     /locus_tag="WJK66_000835"
     CDS             complement(14062..14160)
                     /locus_tag="WJK66_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HEZ7578577.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
     gene            complement(14205..>14576)
                     /locus_tag="WJK66_000836"
     CDS             complement(14205..>14576)
                     /locus_tag="WJK66_000836"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578578.1"
                     /translation="VKEYQPEVGIFLFVCFEKCLFPDFAIPIRFCAVRCVLARTCSKS
                     DVVPVFEALIRDADFQYIFLSYNNEGLMSVGQVREIFERFGKYDLVQTEYRRFKADKT
                     ENRNHKANSTFEYLHILEKTF"
BASE COUNT         3451 a         3985 c         3949 g         3191 t
ORIGIN      
        1 tttggaacgc cttgccgccc gcgatattat ccgtttcccc ggttttgtga acaatgtttc
       61 cgaagtgata aaagaacatc atatattcgt attgccgtct tattataggg aaggcgttcc
      121 ccgaagcact caggaggcaa tggccgtcgg cagggcagtg attacgacgg atgtccccgg
      181 atgcagggaa acggtcgccg acaaggtcaa cggcttcctg atcgagcctt ggaatccccg
      241 catcttggcc gaaaaaatga tttattttat cgaaaacagg gaagccgtcc gcctgatggg
      301 gaatgcaagt tatgcgattg ccaaagataa attcgatgcc gaaaaagtcg atttgaaatt
      361 actcgatatt ttgaaggcgt aaacaaggct gcccgctttt gagtttcggt catttctgat
      421 aaacccccgt cattcccgcg caggcgggaa tccaggttcg tttagtttcg gtcatttccg
      481 ataaattctt gcagctttgc gtttctagat tccctctttc gcgggaatga cgggatgcag
      541 gtttccgcgc ggacggattc ggcattcccg ctttcgcaga aatgccggct tttggtttgt
      601 tgtctttata agattattca gcaattattc tgcggttttg cgtttgtgtg ctgccggaat
      661 ctcaagggat tctctgtatt tggcaatggt gcggcgagaa acctccatgc cgcggaaagc
      721 cagcaggttg gcgagtgcct cgtcagaata cggcgtgggt gtggacgagg caaaaccggc
      781 ggcgggaaag ggattgagcg aagcaatgag gtcgagtgcg gcttcgagtg tgccgctgtc
      841 ggtttggggc aggtgttttt ttattcgtgc gagggtttgg ctgcggttgc cgtcaatgct
      901 gtcgagggcg tttcggacga tatgcagggc ggcgggtttg gcagcacatt cgcccaatct
      961 ttctatctgc agtatcagcg attcgttcaa atcggcggcg gccacgcctg ccgggtcgaa
     1021 ttttttcaat gcggtcagcg cgtgttgcag cattgcttca tccaacatcc actctaaggg
     1081 cgtatggtcg aggatgtctt cgatgctgtc ggtcagataa ccctgctcgt caaggaaatc
     1141 gataaggatg tggacacagg cggcttcttg gtcggaaagc gggtgttcgc atacttgcgc
     1201 gtgcaggtat tgcttgaaat cctgctcacc ggcgatgttg gacagcatat cttcgccttc
     1261 gtctccgccg atttgacggg caggcgcggt gtaatggctg aattcggcat cggaaaattc
     1321 atccgtgtct ttgcgttcga gcaggggatt gtccgacagc cagttttcga cctcgcgttc
     1381 aagttcgata cccgacatct gcaatacgcg caaagattgt tgcagccgct ggttgagctg
     1441 ctgggtctgt ttgagcttta ttccgagtaa ggtcatgata atgtgttgga aattgttatt
     1501 ttcagcctgt cggcgcaaaa aaatgccgca aagcgtcatt gcattataaa tggttttaat
     1561 gagcgggttc ggattctgtc tgataacaaa aaacaaacga aaatcaaaaa ccgattgctt
     1621 ataacaatat taaatcgatt tcatagtttt aatagcgaaa atcttggcgt atagtcgcat
     1681 ccatagtttt tacaaaagga aaacaaaatg tcgattcaag aaatttattg caatcaagaa
     1741 accggttacg aatacgcttt ccgccaaatc gtactgtaag ccgtttaccc ccatttgacc
     1801 aacctgagaa aaggaacaag agcgatgact acctccaaat gccctgtaac ccatctgacc
     1861 atgaacaacg gcgcgcctgt tgccgacaat caaaacagcc tgaccgccgg tcctcgcggc
     1921 cctctgctgg cgcaggattt gtggctgaat gaaaaactcg ccgacttcgt gcgcgaagtc
     1981 atccccgaac gccgtatgca cgccaaaggt tcgggcgcgt tcggtacgtt taccgtaacg
     2041 cacgacatca ccaaatacac ccgcgccaaa atcttcagcg aagtcggcaa aaaaaccgag
     2101 atgttcgccc gtttcaccac cgtggcaggc gaacgcggcg cagccgatgc agaacgcgac
     2161 atccgcggtt ttgccttgaa attttatacc gaagaaggca actgggatgt ggtcggcaac
     2221 aacacgcccg tgttcttcct gcgcgacccg cgtaagttcc ccgacctgaa caaagccgtc
     2281 aaacgcgacc cgcgcaccaa tatgcgctct gccacaaaca actgggactt ctggacgctg
     2341 ctgcccgaag cactgcacca agttaccatc gtgatgagcg accgcggcat ccccgccggc
     2401 taccgccata tgcacggctt cggttcgcat acctacagct tctggaacga agcaggcgag
     2461 cgtttttggg tgaaattcca tttccgcacc caacaaggca ttaaaaacct gaccaacgaa
     2521 gaagccgcca aaatcatcgc cgacgaccgc gaaagccatc agcgcgactt atacgaagcc
     2581 atcgaacgcg gcgagtttcc gaaatggacg atgtacatcc aagtcatgcc tgaagcagac
     2641 gcggaaaaag taccttatca tccgtttgac ttgaccaaag tttggccgaa aaaagactat
     2701 ccgctgattg aagtgggcga attcgagttg aaccgcaatc ccgaaaactt cttcgccgat
     2761 gtggaacaat ccgccttcgc accgagcaac ctcgttcccg gtgtcggcgc cagcccagat
     2821 aaaatgttgc aagcccgttt gttcaattac gccgacgcgc aacgctaccg cttgggcgtg
     2881 aacttccgcc aaatccctgt caaccgtccg cgttgccctg ttcacagcaa ccagcgcgac
     2941 ggacaaggac gcgccgacgg caactacggc agcctgccgc actacgaacc caacagcttc
     3001 ggccaatggc agcagcagcc cgacttcgcc gaaccgcctt tgaaaatcaa cggcgacgcg
     3061 gcacactggg actaccgcca agacgatgac gactatttca gccaaccgcg cgccctgttc
     3121 aacctgatga acgacgcgca gaaacaggca ttgttcggca acaccgccgc cgcaatgggc
     3181 gacgcgcccg acttcatcaa ataccgccat atccgcaact gctaccgttg cgatccggca
     3241 tacggcgaag gcgtggcaaa agccctcgga ctgactgtcg aagacgcaca agccgcccgc
     3301 gcgaccgacc ccgcactcgg ccagcccggt ttgctgtaag ggggcattat gtggatggaa
     3361 attgaagaaa tcctgtcccg cgccgtccgc taccggaaat aaccgggcat aaaaatgccg
     3421 tctgaaacgt cgtttgaccg cttcagacgg catttccaca ccccaccccg catttttata
     3481 tggtcgcgca tcgcagtggg acgtagggtg ttcgtagggt gggcttcagc ccaccaatca
     3541 caccaatcac accaatcaca ccaatcacac caatccgcca cctcgacaaa tccgccacct
     3601 cgacaaatcc gacaaatccg acaatcttac cggtgccggc aaacctatgc cgtctgaagt
     3661 tggcggaggc ggtgggctga agcccaccct acagcccgcc attcccgccc cacagccaac
     3721 ttttccgcgt cattcccgcg aaagcgggaa tccaggttcg ttgagtttca gccatttcta
     3781 ataaattctt gcaactttga gtttctggat tcccgcctgc gcgggaatga cgaaatttca
     3841 gtttttgatt ttttgttttt gcgggaatga cgggatttga gattgcgggc atttatcggg
     3901 aaaacagcaa cctctcctcc gtcattcccg cgcaggcggg aatccggacc cgtccgcacg
     3961 gaaacttata tttcgtcatt ccgccaatcc caccccaccc ccaaaaaaac caaaaaaaac
     4021 ccaatctgac aaaatctgac aaaaaacgtc agcccgaaac tgacaaaaat tgtcacaatt
     4081 gacaaaaaaa cgtcagcccg ccgcaggccg cgccgcgttg atataaaaaa ataccaatac
     4141 caaacagaca aataaaaaaa tattctgtaa aatgccgagc tgaaccccgt tttggcacgc
     4201 cgcttgctgg aaagaaggcg aaccgaaaca aaaaagacca cggtcaaata cattacgggt
     4261 ttacgtttgc ggcggatgcc gcaaatttcc aatcaaactt aaacattttc aaacaggagt
     4321 catccaaatg aaagcaatcc aaaaaggttt cactctgatc gagctgatga tcgtcatcgc
     4381 catcgtcggt atcttggcag ccgtcgccct gcccgcatac caagactaca ccgcgcgtgc
     4441 ccaaatgtcc gaagccctga ctttggcgga aggtcaaaaa accgcagtgg tcgagtatta
     4501 ttccgacaac ggcgtgttcc cgaaaaacaa tgcttctgcg ggtgttgcca gtgcttctga
     4561 gattgtaggt aagtatgtgg ctaaggtaga cgtttcgggt aataccatta cgtctacaat
     4621 gaaaacttcc ggtgtaaaca aagatatcag cggagcaacc cttgtactgg aaggcgcgca
     4681 aaaatctggc tcgttcacgt ggacttgtaa agcgggttct tcaaatggtg tgaaagacaa
     4741 attcctgccg tcttcctgcc gcgccaaata aggacaatga ccgggtctga cccggccgtg
     4801 aaaacaaatg ccgcccgaac cgctcgggcg gcattttcta ttcccctttg ccgctgtagg
     4861 gtgggcttca gcccacccta cgaaacaccc tacgaaacac cctacagcct acacacccgc
     4921 cctacgaaac gccctaccca tttccctgca aatcccctaa aaatccgccc gcacgccctg
     4981 aaaacaagga ataatacagg tttccgccca tcgggctaaa aaggggataa atatgtcaaa
     5041 ttaccggagg gacttcacgc gcggggcgca ctattttttc acattggctt tgcaagacag
     5101 gacgaaggat tacctgatac ggcatatcga caggctgcgc ctcgcctatc gtgaaacgaa
     5161 aacacgttat ccgtttgaaa cggcggcaat ttgtattttg ccggatcacc tgcatttcat
     5221 tatgcggctg ccggaaggcg accacgatta cccgatacgg attgcatatt tgaaaacccg
     5281 gttttcccaa ctcatcccca aagagtgccg cgcccccaac gggagtaaaa caaaaaaacg
     5341 cgaagccggc atttggcagc gcaggttttg ggaacacctg atccgggacg aacaagattt
     5401 aaacaaccac cgggattata tttatttcaa ccccgtgaaa cacgggtatg cggcgagtgt
     5461 gaaggaatgg cgttattctt ccttcttcaa agacgttgaa aaaggcattt accctttgga
     5521 ttggggaggg taaagctccc gccgccgcca aaatccgcag gacggacgcg gggcagatga
     5581 gtcgggcggg acgtagggtg aggcgtaggt gcgtagggtg ggctgtaggg tggatgtgta
     5641 gggtgggctt cagcccacca atccaaccac ctccgccaat ccaaccattc ccaccaaccc
     5701 aaaccaacca ttccgccaat ccccgccaaa aaccgaaacc gccgaatccc gacggtttgc
     5761 caaaaaccct tgatgcggtg gaattggcgg gattggatga gttggtttgg cagaattggt
     5821 gggctgaagc ccaccctaca gcccacccta caacccgccc taagtcccac ctacgttcca
     5881 tccgacgcat ccgccctacg gttccggatt cccctgcgaa ccgccccgcg cacgaaaact
     5941 gctttacgcg gcgtttttcg cgttttgctg ttttgctata atccgccctt tttaaggacg
     6001 ggtgcggtat gagtctttac gctttgctct tgattgcgct ggggatgtcg atggatgcgt
     6061 ttgccgtcgc gctggcaaag ggtgcggcgg tcaggatgcc tccgcgcaaa attgcagcaa
     6121 cagccttggt gttcggcacg gttgaagcgc tcacgccgct ggcaggctgg gtaggcggtt
     6181 tttatgccaa gccgtttatc agcgaatggg accattgggc ggctttcgtc ctgctgggcg
     6241 ggctgggtct gaaaatgatg cacgaagggt tgtccggcga ggcggaggac gcgcgggaaa
     6301 acaaacggga aagctggtgg ttgacggttc tgactgcttt tggaaccagt attgattcca
     6361 tgatagtcgg ggtgggcttg gcgtttatgg aggtaaacat cgcctttgcc gccgcaatca
     6421 tcggtatggc gacgacggtg ctggtggcgg tcgggctgac ggtgggaagg gctttgggcg
     6481 tattgttcgg caggcgtgcg gaatttgccg gaggtttggt gttgattgcc atcggtacat
     6541 ggacgctctt gtcgcacttg gggctgatcg ggtaaggact gccaaaaaat gctgtctgaa
     6601 gcgttgtttg accgtttcag acggcatttt ttattttttg ggcgtagggc gaatccgtaa
     6661 ggtgggatgt aaggtgggat gtagggtggg cttcagccca ccctacgccc actgtttttg
     6721 attttttgtt tttgcgggaa tgacgggatt ttggcttact gtttttgtga aaatggcgaa
     6781 accgcgcaaa tgctgatgcc gtctgaaaca ctgtttgatt gtttcagacg gcattttttg
     6841 ttcacatcga tcaagccgcg ttgtcgaaac cgctgtggcg caagagtgcg tctatgctcg
     6901 gttcgcggcc gcggaaggct ttgaaggatt ctgccgcgct gcgcgatccg ccgacggcga
     6961 ggatttcctg ccaaaagcgt ttgcctgtgg cggcgacatc gtcgctttct tcaaaggcgg
     7021 cgtatgcgtc cgcgctcaat acttccgccc acgcgtagct gtaatagcct gcggaatagc
     7081 cgcctgcgaa gatgtggccg aagctcaagg tgaagcggtt gtattcgggc ggctggatga
     7141 cggcgacttt tttgcgcacg ctgtctaaaa cctgttgcca gtttttcaga cggccttcgt
     7201 cgtcttcgct gtaaatcatc atatcaaaga gggcgaactc catttgccgg acgaggaaca
     7261 tgccgcgttg gaagtttttg gcggcgagca ttttgtcgaa gagttctttc ggcaggggaa
     7321 cgccggtttc ttcgtgggct gacatttgtg ccaagacatt gtattcccaa acgaaatttt
     7381 ccataaactg gctgggcagt tcgaccgcgt cccattctac gccgttgatg ccggatacgc
     7441 ccagttcgtc cacttgggta agcaggtggt gcagcccgtg tccggtttcg tggaagagga
     7501 tgaggatttc gtcgtggctc aggcgggctt ccctgccgcc gacgggtggg gcgaagttgc
     7561 agacgaggta ggcggtgggc agttgcagcg tgccgtctga aaaacggcgg cggcctttgt
     7621 agtcgttcat ccacgcgccg ccgcgcttgc cttcgcgggc gtagaggtcc atatagacgc
     7681 cgcctatggt tttgccgttt tgttgcaatt caaaatagcg cacgtctttg tgccagacgg
     7741 ggacggtttt ttcggtaaat ccgatgccgt agagtttttt gatttgggcg aacagtccgt
     7801 ttaatacttt gccgacgggg aagtattttt tgacttcggt ttcgctgaac gcgtatttgg
     7861 cttcgcgcag tttttcgctg gcgtagccca agtcccacgg ttgcaaatcg gcgaggccga
     7921 ggctttcgcg ggcgaaggct ttgacttcgg cgaggtcttt ttcggcgtag ggtttggcgc
     7981 ggcgggcgag gtcgtgcagg aagtttaaaa cttgttcggg ggtgtccgcc attttggttg
     8041 ccagcgacaa ttcggcgtag tttttgaagc cgagcagttt ggcggtttgc agggcgtttg
     8101 cgagcgtgcg gtcgatgttg gcggtgttgt cgaatttgcc gtcgtctgac agttcgctgg
     8161 cgcgggtaac gtaggcgcgg tagatttgtt cgcgcagttc gcggttgtcg gcgtattgga
     8221 tgacggcgag gtagtgtgga atctgcaagc cgattttgta gcctgttttg ctttcgcttt
     8281 gcgcggcggc ggcaaacatg gcgagcgcgt cttcgggaat gccggcaagc ggtgcggcat
     8341 cgtcaaagta aatgccgaac gcgtcggtcg cgtctaggac gttttgggag aatttggcgg
     8401 aaagttgcgc gccttcggtt tgcagttttg ccagttctgc ctgctgttcg ggcggcagtt
     8461 ccgcgccgct gaggacgaaa tcgcgcagat cgtggttgag tttggttttt tgtgcggggg
     8521 agagggtgtc gaattcgggg gaatttttga tggttttgaa gcggttgtac agctcgatgt
     8581 cttgtccgat ttcggtgaag aagacggtga tttcgggcat cagttcgtta tagacggcgc
     8641 gcagttcggg cgtgtcggcg acggagttga ggtgcgacac cacgccccaa atcctgccga
     8701 cgcgttcggt gatgccggtc aggggttcga cagtgtttgc ccagccggtg tgcgtttggg
     8761 ctttgatggc ggcgatttgt tcgcgcgctt cggcgatggc ggtttgcagg gcgggtttga
     8821 tgtcttcggt tttgatttga tcaaaacggg gttcttcgcc caaatggagc agtgcgttgt
     8881 cagtcataag atgggtttcc tttgcgtgtt gtgcttcaga cagcatatcg gcaatatgcc
     8941 gtctgaagcc ggaaatgggg tcggtcgggg aaagttaaag tgtttcggag ccggcggggt
     9001 aatttaacag gcggtattgt acgcgcaaaa attcgtccat aaccgcctgt tggatttcca
     9061 gccgctttga aacgggggag gcgaagcgga cgatgatgcg gtatgccttg tcatcgtacg
     9121 gcacgcgggt aacgcgcggt ctggcggcgg gcgtgataaa cagtttttcc gcctgcacgt
     9181 tttccaaatg ccgttggatg gcggggatgt agggcgcgca caagggctcg agtacggctt
     9241 tcagacggca tacggcttca tccgaatcca aatggatggg gacggggatt tcgaccgtat
     9301 ggatgacata gtcgcccaaa atattgtcgc ggcgcacggg gtggctcaac aacaggctgt
     9361 tggggaaaga aacggtggtt cccgcaagct gtccgaccaa ggggttcgga ccgacctgca
     9421 tcatcagcgt gttcaacagg ttgatgtcga ccacgcgccc gcgcaggccg ttgatttcga
     9481 tatagtcgcc gaccgagtat tgctgggtgg cagaccttaa aatactgccc gacagacaca
     9541 taatcagttc cttcgtcgcc acgacgaccg ccgccgccac cgcaaacatc gacaaagcca
     9601 gcgtttggat ttgcgccgac cagataaatg ccagcgaaaa cagcaccaaa agcagcgtta
     9661 tattgcggct ggcaaccaaa aaccgccgct tgctttcgat gccgaaatcc ggatgccgtt
     9721 tgaagtggat attcaacaga agggcgcgcg ccagcagcaa agccgcaacc gccgccacgg
     9781 attcgaccgc ctccgcacgt atcgggacgg caccgagcca agtgtccaac ctattccata
     9841 tttccatttc cgcgcccctg tccgaaaagt atagggtgta gattttagtg gcaaaaaaac
     9901 gcttttgcca ctttacggga aaatccccga cctgatgccg gaggcaaacg taccgtttca
     9961 tcttttcccg gcggcacact gatgcggata acgctgaatt aggcggcaaa atcggctata
    10021 atacacaatt atatatgccg tcccgcccgc tttttccaca aggtgcggac ttgttttggc
    10081 ggcatcgcaa aatcttttca aaatccggca aaaaatatga ctgaacaaaa acacgaagaa
    10141 tacggcgccg acagcattca ggtgctcgaa ggcttggaag cggtacgcaa acgccccggc
    10201 atgtacatcg gcgacacgca ggacggcagc ggcctgcacc atatggtgtt cgaagtattg
    10261 gacaacgcca ttgacgaagc actcgccgga cattgcgaca aaatcacggt aacgatacac
    10321 gccgaccatt ccgtcagcgt cgccgacaac gggcgcggta tgcccaccgg catccacccg
    10381 aaagaaggac gctccgccgc cgaagtcatc atgaccgtat tgcacgcggg cggtaaattc
    10441 gacaacaaca gctacaaaat ctccggcggc ctgcacggcg tgggcgtgtc cgtcgtcaac
    10501 gcgctgtccg actgggtaac gctgaccatc taccgcgacg gcaaagaaca cttcgtccgc
    10561 ttcgtgcgcg gcgaaaccga agagccgctg aacgttgtcg gcgattccga taaaaaaggc
    10621 acgaccgtgc gcttcctcgc cagtacggaa accttcggca acgtcgaata cagcttcgac
    10681 atccttgcca aacgcatccg cgaactttcg ttcctgaaca acggcgtgga catcgaattg
    10741 accgacgagc gcgacggcaa acacgaaagc ttcgcccttt ccggcggcgt ggctggattc
    10801 gtgcaataca tgaaccgcaa aaaaacgccc ttgcacgaaa aaatcttcta cgcgttcggc
    10861 gagaaagacg gcatgagcgt cgaatgcgca atgcaatgga acgacagcta tcaggaaagc
    10921 gtgcagtgct tcaccaacaa catccctcag cgcgacggcg gtacgcacct gaccgcgttg
    10981 cgccaagtga tgacgcgcac catcaacagc tacatcgaag ccaacgaagt cgccaaaaaa
    11041 gccaaagtgg aaaccgccgg cgacgatatg cgcgaaggtt tgacctgcgt gttgtccgtc
    11101 aaactgcccg accccaaatt ttcgtcccaa accaaagaca aactggtttc cggcgaaatc
    11161 ggtcccgtcg tcaacgaagt catcagccaa gccctgaccg acttcctcga agaaaaccct
    11221 gccgaagcca aaatcatcac cggcaaaatc gtcgatgccg cccgcgcgcg cgaagccgcc
    11281 cgcaaagccc gcgaaatcac ccgccgcaaa ggcgtaatgg acggcttggg gctacccggc
    11341 aaactcgccg actgccaaga aaaagacccc gcattgtcag aactttattt ggtcgagggc
    11401 gattctgcag gcggttccgc catgcagggg cgcgaccgca aattccaagc gattttgccg
    11461 cttaagggca agattttgaa cgtggaaaag gcgcgctttg aaaaaatgct cgccagtcag
    11521 gaagtcgccg ctttgattac tgcgttgggt gcaggtatcg gcaaggaaga attcaatgcc
    11581 gagaagctgc gttaccaccg catcatcatt atgaccgatg ccgatgtgga cggcgcgcac
    11641 atccgcaccc tgcttttaac cttcttctac cgccaaatgc ccgagctggt cgaacgcggc
    11701 tacatctaca ttgcccagcc gcctttatac aaagccaaat acggcaaaca agaacgatat
    11761 ctcaaagacg aattggaaaa agaccaatgg ctgctcggtt tagccttgga aaaagccaaa
    11821 atcgtttcag acagccgcac catcgaaggc gaagagcttg ccaataccgc caaacaattc
    11881 ctgttggcca aaaccgtcat cgaacaggaa agccgcttcg tagacgaact cgtcctgcgc
    11941 gccatgctac acgcgtcgcc cattgatttg acgtcgtctg aaaacgccga taaagccgtt
    12001 gccgaacttt ccggtctgct tgacgaaaaa gaagtcgccc tcgaacgcat cgaaggtcat
    12061 gaaggacacc ggttcatcaa aatcacgcgc aagctgcacg gcaatgtggt ggtgagctac
    12121 ctcgagccta agttcctcaa cagcaaagcc tatcaaaccc ttacccaaac cgccgccgca
    12181 cttaaaggca tggtcggcaa aggggcgaag ctctacaaag gcgacaacga atacgacatc
    12241 gacagctttg aaggcgcttt ggacatcttg atgagcgttg cccaaaaagg tatgtccatc
    12301 caacgataca aaggcttggg cgagatgaac cccgaacagc tgtgggaaac cacgatggat
    12361 cccgccgtgc gccgcctgtt gaaagtgcgc atcgaagatg ccattgccgc cgacgaagta
    12421 ttcgttaccc tgatgggcga cgaggtcgaa ccgcgccgcg cctttatcga aaacaacgcg
    12481 ctgattgccc aaaatatcga cgcataagtg ccgttttaaa aaaggagacg ggcatcgtgc
    12541 cgcgtctcct tttggtttgt caaacggaag ccatgccgtc tgaaaccgcc tttcggagca
    12601 aaaccatggt cagcattttt gatattttca aaatcggtat cgggccttcc agttcgcata
    12661 cggtcggccc gatgaaggca gccgccgcct ttgcggcagg tttggatgca caggctgttc
    12721 gcatcgtcat cgacatttac ggctcgctcg ccctgaccgg atacggacac ggtacatttg
    12781 acgcgctgat gctcggtttg gaaggcagcc tgccgcacga catcccgctt gtcggcattc
    12841 ccgaacgcct cgaacgcatc cgcacgcagc acatcctccg gctcaacggg caagaaatcc
    12901 gcttcatccc cgaccgcgac ctgaacatac tcggcaatca agtgctgccc aaacacccca
    12961 acggcctgcg ttttaccgcc tatgcttcag acggcacggt attgaatgaa caggtttatt
    13021 attcggtcgg cggcggcttt gtcgttaccg aagaagattt tgaccggcag gcggaaacgg
    13081 aaaaagccgt tccctatccc tataccagtt gcgccgaact gcttgcccga tgccgtctga
    13141 accggctcga catctccgaa gtcgtgttgg caaacgaagc cgcgcttgcc ggatgcggcg
    13201 aagccgaaat ccgccgccgc gccgctgccg ttgccgaggt tatggaaggc tgcatcaaac
    13261 gcggcttggg tgcggacggc gaactgcccg gcggattgaa cgtccgccgc cgcgccccgc
    13321 agcttgccgc caagctcaaa gtcctgcgcg aaaccgaaat cgtcaacacc cagctctggc
    13381 cgatggtgta cgccatggcg gtcaacgaag aaaacgccgc cggcggacgc gtcgttaccg
    13441 ccccgaccaa cggggcggca ggcatcattc ccgccgtatt gcactatttc cgcaagttca
    13501 acccccacgc cacacaggaa cgcgtcgaaa acttcctgct caccgcaggc gcaatcggca
    13561 tcctctacaa gaccaacgcc tccatttccg gtgcggatgt cggctgtcag ggcgaagtcg
    13621 gcgtagcgtg ttcgatggcg gcgggcgcat acgccgaagt catcggcggc acgcccaaac
    13681 aagtggaaaa cgccgccgaa atggcgatgg aacaccattt ggggctgact tgcgaccccg
    13741 tcggcggact ggtgcaaatc ccctgcatcg agcgcaacgg catcgccgcc gaaaaagccc
    13801 tcaaactcgg cacgctcgcg cttttggaag acggcacgga caaaaaagtc tcgctcgacg
    13861 aagtcatccg aaccatgctg caaaccggcc gggatatgaa ggcgacctac aaagaaacct
    13921 cgcttgccgg actcgccgcc acgctccgga aaaaagccgt ccccgtatcc gtgcgcgtgg
    13981 tcgagtgctg aggatggaca gaaacgaaaa tgccgtctga aaacagtttt ttagacggca
    14041 tttcatttat ttcttgcggc tttaccgttt cagtgccagc gtcaacacgc ccgccgtaac
    14101 cagccccaag cctatccatt cctgcgtgtt cgggcgttcg tctaagaaaa ccaccgccat
    14161 caggaatgca aaccggcgtt gtttgcagca tccggcaaga atttttaaaa ggtcttttct
    14221 aaaatatgca gatattcgaa tgtcgaattt gccttatggt tacggttttc tgttttatct
    14281 gccttaaacc gccggtattc cgtttgaacc aaatcatatt tgccgaaacg ctcaaaaatc
    14341 tcccttacct gcccgacaga catcaagcct tcgttgttgt agctgagaaa aatatattga
    14401 aagtccgcat ccctaatcaa agcctcaaat accggaacca catccgattt tgagcaagtc
    14461 cgcgctaaaa cacatcgtac agcgcaaaac cggatgggaa tggcaaaatc cggaaacaaa
    14521 catttttcaa agcatacaaa caaaaaaata ccaacctccg gttggtattc cttaac
//
LOCUS       DAWKOG010000037        14476 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.037, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000037 DAWKOG010000000
VERSION     DAWKOG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14476
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.037"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(53..427)
                     /locus_tag="WJK66_000837"
     CDS             complement(53..427)
                     /locus_tag="WJK66_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578579.1"
                     /translation="MIIQNEFNLYPSNMLPERFCYPEKYVRISNDTSLIPYIQPHNFH
                     WWFENYGTEGAEVAYIFRNSILPDLNLIPFASNGEWEAYFDGNDVTGNPRVIVINLDN
                     IENHEFFNSFEEWLELAIKDTW"
     gene            complement(600..956)
                     /locus_tag="WJK66_000838"
     CDS             complement(600..956)
                     /locus_tag="WJK66_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mimic protein"
                     /protein_id="HEZ7578580.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITENQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTESLVKNIIH
                     ALEYAIKNEKTVIFHF"
     gene            complement(1099..1443)
                     /locus_tag="WJK66_000839"
     CDS             complement(1099..1443)
                     /locus_tag="WJK66_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578581.1"
                     /translation="MNLENYENILIKLLFYHNNLVNEYSYFIENKNLFKIVSRTKTSK
                     GFFFTIEKPLNFLSKKTYFEFNFKYLHSGKERFGSFMCWINTNLMEFEGVFFNDLLPD
                     NMIINNFFEIND"
     gene            complement(1602..2003)
                     /locus_tag="WJK66_000840"
     CDS             complement(1602..2003)
                     /locus_tag="WJK66_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578582.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINSDKVEVIAVGYRNTDKTIKIFIE
                     DVIHFRVVDESYFIDTFMDLISEDADRALLHENGGQSFFELLDECYAEWILKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIEG"
     gene            complement(2007..2747)
                     /locus_tag="WJK66_000841"
                     /pseudo
     CDS             complement(2007..2747)
                     /locus_tag="WJK66_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713004.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(2817..3134)
                     /locus_tag="WJK66_000842"
     CDS             complement(2817..3134)
                     /locus_tag="WJK66_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578583.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            complement(3131..3733)
                     /locus_tag="WJK66_000843"
                     /pseudo
     CDS             complement(3131..3733)
                     /locus_tag="WJK66_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253822.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(4159..4503)
                     /locus_tag="WJK66_000844"
     CDS             complement(4159..4503)
                     /locus_tag="WJK66_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578584.1"
                     /translation="MIIEHNGNIHKIARMTGNKNNFLEIILSDIHENIKIKPLTIKVK
                     GENVINILPEEVSFYVKQGVDLIYEKYKRKFFISEISFCQSDSRPSSIYAFLTFHLLE
                     DIIKNESPSNYT"
     gene            complement(4541..4864)
                     /locus_tag="WJK66_000845"
     CDS             complement(4541..4864)
                     /locus_tag="WJK66_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578585.1"
                     /translation="MFLDDVNIFLDDLNTNPITDEWYMSNFADKHIKILESYEAFDIL
                     KQFVDYMIEEYDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQEISQDIL
                     NEIFR"
     gene            complement(4867..5055)
                     /locus_tag="WJK66_000846"
     CDS             complement(4867..5055)
                     /locus_tag="WJK66_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HEZ7578586.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNVNKIEI
                     HYIENTRTGEKTDFKFKD"
     gene            complement(5125..5469)
                     /locus_tag="WJK66_000847"
                     /pseudo
     CDS             complement(5125..5469)
                     /locus_tag="WJK66_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(5553..5855)
                     /locus_tag="WJK66_000848"
     CDS             complement(5553..5855)
                     /locus_tag="WJK66_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578587.1"
                     /translation="MLNEIFEIYSRQGESLIGIGIREAALPVPIAIDILNLFINERIL
                     VLGGDIYIKKDNYFYQTYDNWYYEGSNLFNSIDKAMHYLSQIKLENAYVSFVLKFI"
     gene            complement(5878..6147)
                     /locus_tag="WJK66_000849"
     CDS             complement(5878..6147)
                     /locus_tag="WJK66_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578588.1"
                     /translation="MMTESHGYLSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYCWLGFDELPQSEKIKFLSYLNILSIHKEIQDETVNRVYTD"
     gene            complement(6168..6374)
                     /locus_tag="WJK66_000850"
     CDS             complement(6168..6374)
                     /locus_tag="WJK66_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578589.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKGLPIR"
     gene            complement(6340..7089)
                     /gene="mafB"
                     /locus_tag="WJK66_000851"
                     /pseudo
     CDS             complement(6340..7089)
                     /gene="mafB"
                     /locus_tag="WJK66_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257312.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(7195..7566)
                     /locus_tag="WJK66_000852"
     CDS             complement(7195..7566)
                     /locus_tag="WJK66_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ7578590.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
     gene            complement(7563..7814)
                     /locus_tag="WJK66_000853"
     CDS             complement(7563..7814)
                     /locus_tag="WJK66_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578591.1"
                     /translation="MDNTRIMAAREAGVKVEANVHNFNDRLSSKERIRFKHDGIEPQT
                     WGEAIQLRIRKQETQKGVPEGWSKRFPNGSIYDVKVLRK"
     gene            complement(7946..8152)
                     /locus_tag="WJK66_000854"
     CDS             complement(7946..8152)
                     /locus_tag="WJK66_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578592.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
     gene            complement(8162..9007)
                     /gene="mafB"
                     /locus_tag="WJK66_000855"
                     /pseudo
     CDS             complement(8162..9007)
                     /gene="mafB"
                     /locus_tag="WJK66_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(9104..9316)
                     /locus_tag="WJK66_000856"
     CDS             complement(9104..9316)
                     /locus_tag="WJK66_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578593.1"
                     /translation="MKLINFEESSVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKIKFFLWIKNLKKYMILGINVS"
     gene            complement(9392..9751)
                     /locus_tag="WJK66_000857"
     CDS             complement(9392..9751)
                     /locus_tag="WJK66_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ7578594.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLNIQD"
     gene            complement(9756..10472)
                     /locus_tag="WJK66_000858"
                     /pseudo
     CDS             complement(9756..10472)
                     /locus_tag="WJK66_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504008.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(10689..11072)
                     /locus_tag="WJK66_000859"
     CDS             complement(10689..11072)
                     /locus_tag="WJK66_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578595.1"
                     /translation="MMTEPHGYPSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYYWLGFDELPQSEKIKFLNYLNILSIHKEIQDKTVNRVYTDCFDIDKLHSLGRI
                     TTNECINGIKKIYQKNKLEFDNILK"
     gene            complement(11038..12483)
                     /gene="mafB"
                     /locus_tag="WJK66_000860"
     CDS             complement(11038..12483)
                     /gene="mafB"
                     /locus_tag="WJK66_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ7578596.1"
                     /translation="MKLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGNARGSVKNRVYAVQTFDATAVSPVLPITHERTGFEGIIGYETHFSGHGHE
                     VHSPFDNHDSKSTSDFSGGVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARD
                     IYSYYVKGTSTKTKTNIVPQAPFSDRWLKENAGAASGFFSRADEAGKLIWESDPNKNW
                     WANRMDDIRGIVQGAVNPFLMGFQGLGVGAITDSAVNPVTYAAARKTLQGIHNLGNLS
                     PEAQLAAATALQDSAFAVKDSINSARQWADAHPNITATAQTALSVAEAAGTVWRGKKV
                     ELNPTKWDWVKNNGYKKPAVRLMQTVDGEMAGGNKPPKPSTQQHSTHSDNNIGLPAPY
                     VKPDTSISPTGTIYVDSKGNAIPTPIGGYITGSPDARFIQVRDSNGNLTGARIDDAHH
                     ANTHQDPRALTGHAHVPGVTNDDGTPWLPIK"
     gene            complement(12523..13485)
                     /gene="mafA"
                     /locus_tag="WJK66_000861"
     CDS             complement(12523..13485)
                     /gene="mafA"
                     /locus_tag="WJK66_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ7578597.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPARSRTQSDGSGSRSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIRPYGNHTGNSAPSVETDNSHEGYGYSDEVVRQHRQGQP"
     gene            complement(13669..14388)
                     /locus_tag="WJK66_000862"
     CDS             complement(13669..14388)
                     /locus_tag="WJK66_000862"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEZ7578598.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDSSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVVTGENEGTLVHC"
BASE COUNT         3950 a         2944 c         2573 g         5009 t
ORIGIN      
        1 cgagtttatc ctagttatcc aggatggtcc catctctttt atagatagct gcttaccaag
       61 tatccttaat tgctaattca agccactctt caaaactatt aaaaaattca tggttttcta
      121 tattatctaa attaatgaca ataaccctag gatttcctgt tacatcatta ccatcaaaat
      181 aagcttccca ttctccatta ctagcgaatg ggataagatt taaatcaggc aggatagaat
      241 ttctaaatat ataagctact tctgcccctt ctgttccata attctcaaac caccagtgaa
      301 aattatgtgg ctgaatataa ggtattaaag atgtatcgtt agagatacga acatactttt
      361 caggataaca aaacctttca ggaagcatat tactaggata taaattaaat tcattttgaa
      421 taatcatttc gttaccttag gatcataaat actaccatta ggaaactggg tagaccaatt
      481 tggtggtgct agttcatttt caatttgttt tttaattcta aattgaatag cttctcccca
      541 tgtttgcggc atctaatgct gcttgctctg catcaatatt actaaaaatt ggattagtct
      601 caaaaatgaa aaattactgt tttttcattt tttatagcat attcaagtgc atgaataata
      661 tttttcacta aggactctgt agaaaaatct gaattttgta atttttcttt tacagccaaa
      721 atactttcct tatctattag ccaatccccc tcacttggtc caaataacca attatcggta
      781 atttcagaaa caagtctcca taatccacga ttttgatatt caataatttg attttccgta
      841 atttctacaa ctgtttcaga ttgaatgttt ttataagccc aatcaatcat ttcagtatat
      901 tcactaattg aaacattgtc ttttaaacag aaaattaaca ggttgtgttc attcatttgt
      961 ctaagttcct gatatgagtt tgttgttgta aataatcttt tgcttgctta ccttgtaaat
     1021 taaaggatta accgcacctt ggatgatgcc gcgaatatca tccatacggt taccccacca
     1081 atttttattg gtgtatcgtt aatcatttat ttcaaaaaag ttatttatta tcatattatc
     1141 agggagcagg tcgttaaaaa aaaccccctc aaattccatt aaattagtat ttatccagca
     1201 cataaacgaa ccaaagcgtt ctttccctga gtgtaaatat ttaaaattaa actcaaaata
     1261 agtttttttg cttagaaaat ttaatggttt ttctatagtg aaaaaaaagc ccttactcgt
     1321 tttcgttcga gatactattt taaataaatt tttattttca ataaaataag aatattcatt
     1381 tactaagttg ttatgataaa aaagtaattt tattaaaatg ttttcataat tttctaggtt
     1441 catttctagc tcaatttaaa atgatttcat tgttgccgta ccaatgcgaa atttaccatc
     1501 aacaaaattt cccttcactg atacaggtac accatcaact tgggtagttt tattttcagg
     1561 ctgcctgaaa gaaagatagg caacttatta ctcatcttat gctagccctc aataattgaa
     1621 tacgttgcac tagaaccaat tatttctatg aatgtttgct caaacataaa aatatagtat
     1681 ttaaagaatt ctctattcaa aggaaaataa ctttctttca atatccattc cgcataacac
     1741 tcatcaagaa gttcaaaaaa agattgacca ccattttcat gaagcaaagc tctatctgca
     1801 tcttccgaaa ttaaatccat aaaagtatct ataaaatacg attcatcaac aaccctaaaa
     1861 tgaataacat cttcaataaa aatttttatg gttttatccg tatttctata accaactgct
     1921 ataacttcta ctttatcaga gtttattgct gcattatcaa aatagaaata gccagttggg
     1981 aaatctaagc tttttaattt cataatttat cgtgtatttc catatctgat ttttattttt
     2041 cggttattac taattgttat ttcaagtgtt ggttctccac cactactatc attacgagca
     2101 ttaaccgcac gcccatcagg taaattgccc gttatcgttc cattaggacg tgtttgtaca
     2161 tttcgaacat tcagactatt aaaatcattt agtgcgtcat tgtaatcccc cactttatca
     2221 tactgaacag cgggaccttt tgttttactc ctttgagtag tattgctcaa aatgtctttg
     2281 atttgtaaat cactgcgcgc cttaccggct tctatgcgag ctgccacact actcgggcta
     2341 taggggtcgc tatcaatcgc aggattacca ccgctggttt tttgtccgct agcctgtttc
     2401 ccaacctgct gtactgtatc gccaatttca tctaccgtac ctaacgcctt agtctgcata
     2461 tggcgaacag caggtttttt atagccggta tttttaaccc aatcccattt ggtcgggtta
     2521 agttctactt ttttacctct ccaaaccgta cctgcggcct ctgctacggc aagggcagtt
     2581 tgggctgttg ctgttatatt cggatgggca tcagcccatt gtctggcgga attgatgccg
     2641 tcttttaccg caaaagcact gtcctgtaat atgctcgcgg cagcaagttg tgcttccggg
     2701 cttaattttc ctaaattatt aataccttgt agagtctgct gcgcagagat gtagcaactg
     2761 tatttttacc ccgtcgggta aaaatacagt tgctacggct gctgggaaag cagttactat
     2821 tttcccagtg gctcaaaatt aattgtttca gcagatattt ttataataat ccctacatca
     2881 ataataattt cactataaaa ttggtattta tatagctcta tttcgggtat aaacaaggga
     2941 aacaagctat cttccttatc ctcataatct acgaaattat ttgtatctaa tttaaaattc
     3001 aaaatattac tgcatttgat atatccgaaa taattacctt caattgtatc tgtaaaagat
     3061 aatgtaaggg tatttccatt atcggaaaag attatttcat aaaggcttac attgtgaaaa
     3121 atattttttt tcatttgata aaatatccag aatgatgacg attatttggc aatggattgc
     3181 cttcataatt ttgtcgttgc ggatattttc cctgtacacc atcggttcgc ggcactcttt
     3241 gggttcttat aacctctcca tttggacgaa tatctatttt cacgccatca ggacctttcc
     3301 atgtttcata ttgagcctca tctgaaccag tacgtctaaa accttgttct tgcaatgctt
     3361 gacgtaattc atctcgcgta gttggtctga tagatttaac aactggcttg tttcctcctg
     3421 ccatctcacc gtccaacgtc tgcatagggc gggcagcagg tgttttatag ccgttatttt
     3481 taacccaatc ccatttagtc gggttaagtt ctactttttt acctctccaa accgtacctg
     3541 cggcctctgc tacggcaagg gcagtttggg cagtagctgt aatgttcgga tgggcatcag
     3601 cccattgtct ggcggaattg atgccgtctt ttaccgcaaa agcactgtcc tgtaataggc
     3661 tcgcggcagc aagttgtgct tccggactta attttcctaa atcattaata ccttgtaaag
     3721 tttgctgcgc agagatgtag gtcggatatt tgtatccgac aaaaatattt gacgcatcta
     3781 tcgtttccga aatgccgctg ttggaaaatg tcggattcga gaatccgacc tacggctatt
     3841 tcgtttaagt cataaatagc cattcatgtt ccttctgaat ttgcttttgc tatccttaga
     3901 accaatcata taaacattat catcattaat taatgttcta agtttatgaa gcggtcaaat
     3961 ctgtcaaact accagagatg aagcatatcc tcctggaagt aattctgttc gtgctgtttc
     4021 catatcttct ttgccatcat cagttacttc tctgtctaaa taaaatctta cgataagttt
     4081 ttgtctaatt tcatcaaatt ctgccgtaat taatcttata ttgatatgaa tacacggttc
     4141 atacctgcta ttagccagtc aggtgtagtt ggatggggat tcatttttaa taatatcttc
     4201 aagcaagtga aatgtaagaa aagcgtagat acttgaaggc cggctatctg attggcaaaa
     4261 agaaatttcg gagataaaga atttccgttt atatttttca taaattaaat caacaccttg
     4321 ttttacataa aaactaactt cctcaggaag gatatttata acattctctc cttttacttt
     4381 tatagttaat ggtttgattt ttatgttttc atgaatatct gataggatta tttctaaaaa
     4441 attattttta tttccagtca ttctggctat tttatgtata tttccattgt gttcgattat
     4501 cattttattt tttccaagga tttccttcta tattgatagt ttatctaaag atttcattta
     4561 aaatatcctg actaatttct tgtttatata attctacaat tttctgtttc tgtgtatttg
     4621 tataaaattt ttcgttggta tctgcttgat attttaattg tctcaatatt tccatgattt
     4681 catattctga tttttcatca tattcttcaa tcatgtaatc aacaaattgt tttagaatat
     4741 caaaggcttc gtaactttcc aaaattttaa tatgtttatc ggcaaaattg gacatatacc
     4801 attcgtcagt gattggatta gtatttaaat catctaaaaa aatatttaca tcatctaaaa
     4861 acatgactaa tccttaaact taaaatctgt tttctcaccg gttcttgtgt tttcaatgta
     4921 atgtatttca attttgttta cgttttgaac acgcttaacc caaccgtctt ttgctaacca
     4981 accattttta gtatcggaca taggaggtat tctcgcagga gttttgccct gtgggtttgc
     5041 cttaacttct tccattgcta attgttcctt taaattatta ggtattgtac gaccggttga
     5101 tttttcaaca gattgtgtcc ctatttttaa tgatttgttt cccccagcca tttccccgtc
     5161 tacagtctgc atagggcggg cagcaggttt tttatagctg gtatttttaa cccaatccca
     5221 tttagtcggg ttaagttcta cttttttacc gcgccaaacc gtacctgcgg cctctgctac
     5281 ggcaagggca gtttgggcag tagctgtaat gttcggatgg gcatcagccc attgtctggc
     5341 ggaattgatg ccgtctttta ccgcaaaagc actgtcctgt aataggctcg cggcagcaag
     5401 ttgtgcttcc ggacttaatt ttcctaaatc attaatacct tgtagagtct gctgagcggc
     5461 tgtatctgag ccggtcagtg taggttggat taaaagccag cattttcaac atcttgatgt
     5521 tttactataa atctattctt gtgcttcctt tgttagataa atttcaacac aaaagatacg
     5581 tatgcattct ctaattttat ttgagataaa taatgcattg ctttgtcgat actgttaaat
     5641 aaattacttc cctcgtaata ccaattatca tatgtttgat aaaaataatt atctttcttg
     5701 atataaatat ctccccccaa tacaagtatt ctctcattga taaataaatt taatatatct
     5761 attgcaatag ggacgggtaa tgcggcttct ctaattccaa ttcctatcaa agattcccct
     5821 tgtctcgaat aaatttcaaa aatttcattt aacatgttaa tctctatcta ctatttttca
     5881 atcggtataa accctattca cagtttcatc ttgtatttct ttatgaatac ttaatatatt
     5941 aagatagctt aggaatttta ttttctcaga ttgaggcaac tcatcaaaac caagccaaca
     6001 ataaaaattg ttatatgatt ttaaaaaatc aacaattttt atttttccta aaaccaaatc
     6061 ttcgactaac tgggttgcat aaataatatc atttatgatt ttaacaggta aattatttga
     6121 taggtaacca tgggattccg tcatcattga tttctctagg tgcttattta tctgattggt
     6181 aagccttttg cgtaagcttc tttcgcctcc gcaagtgtca tttcattagg gccaccatct
     6241 atattagttt ctcctgtatt ttgccactga caaacgtcac agatatcata gtccataact
     6301 tcagttccgc aaacaggaca atgtagccat atttctccgt caatttccca tgtctttttt
     6361 gaattctcca tcataatact tctttcctaa gtttggttta aacattgttt tgattttgaa
     6421 tgtttttgga ttggccaaaa catagatatt gttattgata tcatagcgca ctcgtctatg
     6481 ttccgtctga taacctaata tcttgtccga tgtctgtttt aataacaaat cagacgccat
     6541 tttttggtag tcttcaatgg ttatatcgcc aaattctttt ccatgagctt tgaaatgtcc
     6601 atttaaagat ttctcagtag gaaacttgga ctttgtccat ctgatgcggt cttgaattgt
     6661 tcctgtcgga gaaatagatg tatcaggttt aacatatgag gcaggtaagc cgatattgtt
     6721 atcagagtgt gtagggtgtt gctgcgtact tggttttggt ggcttattcc cacctgccat
     6781 ctccccatct aaagtctgca tagggcgggc agtaggtgtt ttatagccgg tatttttaac
     6841 ccaatcccat ttggtcgggt taagttctac ttttttaccg ccccaaaccg tacctgcggc
     6901 ctctgctgcg gaaagggcag tttgggctgt tgcagttata ttcggatggg catcagccca
     6961 ttgtctggcg gaattaatgc cgtcttttac cgcaaaagca ctgtcctgta ataggctcac
     7021 ggcggcaagt tgtgcttccg gacttaaatt tcctaaatca ttaatacctt gtagagtctg
     7081 ctgcgcagag atgtaggtcg gatatttgca tccgacaaaa atatttgacg catctatcgt
     7141 ttccgaaatg ccgctgttgg aaaatgtcgg attcgagaat ccgacctacg gctgctacca
     7201 accatcattt tcagcttctt ttagccaagt atcaaaattt tcaaaagtct cgtaatattt
     7261 tgtattatct aaattaacta ctataatttt aggattacct gatttatctg aaatatcaaa
     7321 acaggcagcc cactcttggt ttctagcaaa aggtaatagg tttggaatgc cagttatttc
     7381 aaaataaatg tcaattactt ctgatatatt gctttctgca ttttcaaacc accaaggaaa
     7441 agaatattgt tcatcgtagt taatggcatg agtggattga gctaatttta aataactttg
     7501 cggatattta aatcctttag gaagcattgc ttcaggatac ggaacaaaac tattttgttt
     7561 tatcatttcc taagtacctt tacatcataa atacttccgt taggaaatct tttgctccac
     7621 ccttctggaa ctcctttttg tgtttcttgc tttctaattc gtagctggat agcttctccc
     7681 caagtttgag gctcaatacc atcatgctta aacctgattc tctcttttga tgataatcgg
     7741 tcattaaaat tatgaacatt cgcttccact tttactcctg cttcacgtgc tgccataata
     7801 cgcgtgttat ccatactagt aggtgctcca tcaggcatat taacaacatc aaccctgtcg
     7861 ccaacccatg atttttttct ttcatttcgg ctacaataga atcatagttg taatttgcac
     7921 taggctctga caaaaatttg ggttgttaag ttgtcatttt tttataaatt ccagcaaact
     7981 gttgttgtaa attatctact tcatgttttc taacaccaga tacattttca aattctcttg
     8041 aatctacaca tacgcccgca tatatctcat tcaagatagc tcgtaaaaca aaaacttcat
     8101 cttgagttag tgaaatagtt gagttttgat tatttatgga tagtatattc atggcttttt
     8161 cctataaatc atcataatat tttttacctg atgttggtct aaatgctgta cctccatcgt
     8221 cagaattttt atctcggata actattgtcc ctgttttatc atcccaatac gcagttctac
     8281 cgcgaggtaa ctctttcata tttgttggat ggctaacaat attttcaata tgccgagcaa
     8341 aatctgctgg tgaattgata tttaaatccg taaattcttg ttgtcttatg acatgcttgt
     8401 tataagcatg cccactacta atttgttctc caattagttg cgcttgtaaa gacggttgtg
     8461 tggaagcatc tgctttgctg ttggacgtta tagatttagg cggtctattc ccaccggcca
     8521 tttccccatc caaagtatgc atggtgcgaa cagcaggtgt tttatagccg gtatttttaa
     8581 cccaatccca tttggtcggg ttaagttcta cttttttacc gccccaaacc gtagttgcgg
     8641 cctctgctac ggcaagggca gtttgggctg ttgctgttat attcggatgg gcatcagccc
     8701 attgtctggc ggaattaatg ccgtctttta ccgcaaaggc actgtcctgt aataggctcg
     8761 cggcagcaag ttgtgcttcc ggacttaatt ttcctaaatc attaatacct tgtagagtct
     8821 gctgagcggc tgtatctgtg acagggctta ccgcactgtc tgtaattgcc ccaatcccta
     8881 ctccttgaaa acccattaaa aaaggattaa ccgcaccttg gacgatgccg cgaatatcat
     8941 ccatacggtt agcccaccaa tttttattgg ggtcgttttc ccatatcagt tttcctgctt
     9001 catcagcaca gttaggattg gcattgatgc ccacccagtt tcaggcagcc tgaaaaggat
     9061 ttttaatata cggcaaaagg tgggcaacaa gtgcccacct tacctagcta acatttatcc
     9121 caagtatcat atattttttt aagttcttta tccaaagaaa gaactttatt ttttcaacac
     9181 catgaattat ctcaaaatct ctacaatcca cgcatacgcc tgaataaatt tctcctacaa
     9241 ttgcttgaat aatatacaac tcatctttat cgagactaat tttaactgaa ctctcttcaa
     9301 aattaattaa tttcatgaat aattcctttt attttaattc gtcatcaaaa taccgtttgc
     9361 ctgtttttgg tctaaatgct gtccaccatc tttagtcttg gatatttaga ttataatcgt
     9421 caaattccca gtcaaaatat tttgccaaat ttgatataga cttatcttca gacgttgaat
     9481 tacatatttc caatatatca tttatcaatt ttaatatgtg ttcaatatta aaacggtttg
     9541 taacaattga gttttttaat gacatcggtc tatgtatatt tgaaagtaac cattcagtgc
     9601 tatatatata aacataaaaa taatcagcac cattactttc atcaaaacct attgataatg
     9661 tcaaaagtat acaaaaattt tctaacttgt cttgtgggaa atcttccaaa tcaaagtcag
     9721 aacaatcata attttttaat tttaatctca tataattacc taatatttaa atgtccattt
     9781 ttaattttat tcctttttcc atttgaatca atagtataag tttcaaaatt tgcttgaata
     9841 cctgtagttg caaattgtga atttttttct gttggtggcc gatattgtcg agtgccatct
     9901 atacttaacc atccgcctcc actagtttgt cttgcaccct caccaaccca aattttacct
     9961 agcctatctg cctcttcata agttgcagtt cctattggaa aatttttttc actcttatga
    10021 agagctagac tcaatcttgg atcttgagcc gcaatgtttt ttaagttttg cccagttaat
    10081 tgattaacta attgaggata agttgcagca ttggcttttc ctccagacgt tatagattta
    10141 ggcggtttat tcccaccggc catttcccca tctaaagtct gcataggtcg ggcagcaggt
    10201 gttttataat cggtattttt aacccaatcc catttagtcg ggttaagttc tactttttta
    10261 ccgccccaaa ccgtacctgc ggcctctgct atggcaaggg cagtttgggc tgttgcagtt
    10321 atattcggat gggcatcagc ccattgtctg gcagagttga tactgtcttt taccgcaaaa
    10381 gcactgtcct gtaataggct cacggcggca agttgtgctt ccggacttaa atttcctaaa
    10441 tcattaatac cttgtagagt ctgctgcgca gagatgtagg tcggatattt gtatccgaca
    10501 aaaatatttg acgcatctat cgtttccgaa atgccgctgt tggaaaatgt cggattcgag
    10561 aatctgacct acggctgctg actattttct tgtttatata attctacaac tgcaaactac
    10621 tatcaaatct ttttatctct ggctctacac tcagcaagaa tatttttatt ttacaaaaca
    10681 taggctagtt atttgagtat attgtcaaat tctaatttgt ttttttgata gatttttttt
    10741 ataccattaa tacattcatt agttgtaatt ctaccaagcg aatgtaattt atcaatatca
    10801 aaacaatcgg tataaaccct attcacagtt ttatcttgta tttccttatg aatacttaat
    10861 atattgagat agtttagaaa ttttattttc tcagattgag gtaactcatc aaaaccaagc
    10921 caataataaa aattgttata tgattttaaa aaatcaacaa tttttatttt tcctaaaacc
    10981 aaatcttcaa ctaactgggt tgcataaata atatcattta tgattttaac aggtaaatta
    11041 tttgatgggt aaccatgggg ttccgtcatc atttgtaact cctggtacat gtgcgtgtcc
    11101 tgtaagtgcg cgtgggtctt gatgggtatt tgcatgatga gcatcatcta ttctggctcc
    11161 agtcaagttt ccattactat ctcggacttg gataaaacga gcatcaggcg aacctgttat
    11221 ataaccgcct attggtgttg gtattgcatt tcctttgctg tctacataaa ttgttcctgt
    11281 cggagaaata gatgtatcag gtttaacata tggggcaggt aagcctatat tgttatcaga
    11341 gtgtgtagag tgttgctgcg tacttggttt cggtggctta tttcccccag ccatctcccc
    11401 gtctacagtc tgcataaggc gaacagcagg ttttttatag ccgttatttt taacccaatc
    11461 ccatttggtc gggttaagtt ctactttttt acctctccaa accgtacctg cagcctctgc
    11521 tacggaaagg gcagtttggg cagtagctgt aatgttcgga tgggcatcag cccattgtct
    11581 ggcagagttg atactgtctt ttaccgcaaa agcactgtct tgtaatgcgg ttgcggccgc
    11641 aagttgtgct tccggactta aatttcctaa attgtgaata ccctgtaaag ttttccgtgc
    11701 tgccgcatag gttaccgggt ttaccgcact gtctgtaatt gccccaactc ccaatccctg
    11761 aaaacccatt aaaaaaggat taaccgcacc ttggacgatg ccgcgaatat catccatacg
    11821 gttagcccac caatttttat tggggtcgct ttcccatatc agttttcctg cttcatccgc
    11881 acggctgaaa aaaccagagg cggcaccggc attttctttt agccaacggt ctgaaaatgg
    11941 ggcttgaggg acaatattag tctttgtttt tgttgaagtt cctttgacat aatagctgta
    12001 tatatccctt gctcctccgg ggggcggata atcgctgcct tgcggcccgt catatccatc
    12061 ctccggatga atttccgacc ctgtccgatg aagttggtaa acagtaaaac cgccgtctac
    12121 accgccgctg aaatcagaag tgctttttga atcatgatta tcgaacggac tgtgtacttc
    12181 atgtccatgt cctgaaaaat gggtttcata accgataatg ccttcaaacc ctgtccgttc
    12241 gtgtgtaata ggcagtacag gactgaccgc agttgcatca aatgtctgga cggcgtaaac
    12301 ccgattttta acactgccgc gagcattacc aaacagatgg tatttaccac ccggttcgta
    12361 gtgttttgcc tgcatatcat cgcgtaaacg ggcatccaaa ccgttcgcat gactaatggg
    12421 gatttgcagc aacgatattg ccgctgcaaa cagcatcatg aatttttgaa taggcaattt
    12481 cattttgttt tccttggtag caagcggtta tggtcgtgtg aatcaaggtt gcccttgtct
    12541 atgttgtcgc actacttcat cgctgtatcc atacccctca tgactgttat cagtctctac
    12601 ggatggggcg gagttacccg tatgattgcc gtatggtcgg atatcggaga aatcgaccat
    12661 taatccttcc gtcggtttaa ttcctttgct tactttatac ggccccatcc acaatgcgta
    12721 attttcttta taggcagctt caaacgcatt ggttttgggt ttgatgagca attttttatt
    12781 ggttctgtct actgcgaaat attccagttt tgtttgggct ttcagtgttt cggcattgta
    12841 taggtgcatt tcggttctgt tgcgtatcgt tccgaatacg tcgatgttaa taaacacatc
    12901 tgtatcggca ttggcaggag aaacaacgtc tatgccgcgc aggaaaaata cggtctgtac
    12961 caagtgggaa agaaaggcag tgtcgcgcgg gttagtcgtc aaggtttcat ttcgataatc
    13021 ccccatcccg ccaatattta agcccagact gctcctactt ccgctaccgt ctgattgggt
    13081 gcgcgagcgt gcaggggcat taagtgtaga taaagaagtg gttaaacctg tcaaaccgcc
    13141 tgatgttgtt tcagcggtgg tttcgtaacg tggataggtg taatcggtac ggacggcagg
    13201 gctgtttatg tattcgccac gaatcagtgc atcaatggag tagcgacccc ctgtcaaact
    13261 gcctgaacct tggtcgccca tagtggcaat gtacaatgca acttttcgtc cgtgtaatgc
    13321 ctgtaaatcc atgtctttaa cggcagctct ggcagaagcg gccacaagtt cttgttcgac
    13381 cgcaaagcgt ttacctccgc catgcgatgg aatacctgtc agtgtcccgc aggcggataa
    13441 aataaaaact gaaaaaagaa taggtatcag cagccgtgct tgcatagatt ttctcctttg
    13501 atgaaaaaca aattgtatca aaattgtaaa tatagtggat taaatttaaa ccagtacggc
    13561 aaggcgaggc aacgccgtac tggtttaaat ttaatccact ataatctcca tataataaat
    13621 ggctgtctga agcccatatg cgactatatc tgccgacact atggtcaatc agcagtgaac
    13681 cagcgtacct tcgttttcac cggttactac acgtttgagc gagccttctt tagcaatgcc
    13741 gaatactaca atattgagct tgcgttcgcg gcagagggcg aaagcggtgg catccatcac
    13801 tttgaggttt ttcaacaagg cttcgtcaaa agtaatggtt tcgtagcgcg tggcggatga
    13861 gtcttttttg gggtctgcgg tgtacacgcc gtcaacgttg gtggctttga gcatcacgtc
    13921 gcagttcatt tccgcaccgc gcaatgcggc ggcagtgtcg gtcgtgaaga acgggttacc
    13981 ggtaccggcg gcaaaaatca cgactttgcc ttcttccaaa tattggatgg ctttaggacg
    14041 ggcgtaagtt tcagcgattt gctgcataga cagtgcggat tgtacgcgcg ctttgatgcc
    14101 taaagtttca aatgcgtctt tgagtgccaa cgcgttcatc acggtcgcca tcatgcccat
    14161 gtagtcggcg gtggcgcgat ccatgctgcc tgcttgggcg gatacgcccc ggaaaatatt
    14221 gccgccgccg acaacaatac cgacttgcac gcccatttta acgacttcgg caatttcgcc
    14281 gacagtttga acgatggtat cgtgattgat gccgaacgga tcggaaccca tcagggattc
    14341 gccggagagt ttcagtaata cgcgtttgta tttgatttgc tgtgtcatgg gataccttgc
    14401 tttcttgaat gtcgtcggtc ggagtttggg ccgtctgaat tttcaaacgg tcggaattac
    14461 aatgatatgg tgtgtc
//
LOCUS       DAWKOG010000038        14429 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.038, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000038 DAWKOG010000000
VERSION     DAWKOG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14429
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.038"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(338..4507)
                     /locus_tag="WJK66_000863"
     CDS             complement(338..4507)
                     /locus_tag="WJK66_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HEZ7578599.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDRFETGKISMGKAFDKQTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGSAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPSAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGSFVIRQDGTVHIGNTSVALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDKKTGISAKGGAHGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLDIGNAFGGNMANAPLGGRITASLPDLGTLKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSD
                     KKDSAGNSKGK"
     gene            complement(4562..6409)
                     /locus_tag="WJK66_000864"
     CDS             complement(4562..6409)
                     /locus_tag="WJK66_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HEZ7578600.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     NKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLHSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKAYPLAKLGNTRAAVNPDTATADL
                     NVVVDSGRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDIDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGAFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNANVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6974..8203
                     /gene="sstT"
                     /locus_tag="WJK66_000865"
     CDS             6974..8203
                     /gene="sstT"
                     /locus_tag="WJK66_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEZ7578601.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALVVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAYTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            8315..9808
                     /locus_tag="WJK66_000866"
     CDS             8315..9808
                     /locus_tag="WJK66_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ7578602.1"
                     /translation="MFKRSVIAMACIVALSACGGGGGGSPDVKSADTLSKPAAPVVTE
                     DVGEEVLPKEKKDEEAVSGAPQADTQDATAGKGGQDMAAVSAENTGNGGAATTDNPEN
                     KDEGPQNDMPQNAADTDSSTPNHTPAPNMPTRDMGNQAPDAGESAQPANQPDMANAAD
                     GMQGDDPSAGENAGNTADQAANQAENNQVGGSQNPASSTNPNATNGGSDFGRINVANG
                     IKLDSGSENVTLTHCKDKVCDRDFLDEEAPPKSEFEKLSDEEKINKYKKDEQRENFVG
                     LVADRVEKNGTNKYVIIYKDKSASSSSARFRRSARSRRSLPAEMPLIPVNQADTLIVD
                     GEAVSLTGHSGNIFAPEGNYRYLTYGAEKLSGGSYALSVQGEPAKGEMLAGTAVYNGE
                     VLHFHMENGRPSPSGGRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAVIDGNGFKGT
                     WTENGGGDVSGRFYGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(9883..10098)
                     /locus_tag="WJK66_000867"
     CDS             complement(9883..10098)
                     /locus_tag="WJK66_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578603.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLKAELFFKITLTMAGL
                     FVIILAALLVFGQYFSEKKMKDDGFIN"
     gene            complement(10091..10307)
                     /locus_tag="WJK66_000868"
                     /pseudo
     CDS             complement(10091..10307)
                     /locus_tag="WJK66_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
     gene            complement(10382..11725)
                     /gene="argG"
                     /locus_tag="WJK66_000869"
     CDS             complement(10382..11725)
                     /gene="argG"
                     /locus_tag="WJK66_000869"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEZ7578604.1"
                     /translation="MNQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEIEPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEDAAFTPLDRIGQLTMRNLDITDTRVKLGIYSQSGLLSLGEGSVLPQLGNKQ"
     gene            11850..12650
                     /locus_tag="WJK66_000870"
     CDS             11850..12650
                     /locus_tag="WJK66_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEZ7578605.1"
                     /translation="MNAFNLIIIGDEILHGSRQDKHFVFFKSLLESKGLKLNQVQYLP
                     DEPDLLVKQLRRSFSDGMPTFVTGGIGATPDDHTRQAAAAALDLPVVRHAQAAGFIEG
                     VTLKRGEPLDSPEHAQRLKMADFPQGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLDTYYAGRFNQTQRGSRSVYVFEQPESRITPIMEHIEQTYLGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLEGLGAELKNRVN"
     gene            12690..13676
                     /locus_tag="WJK66_000871"
     CDS             12690..13676
                     /locus_tag="WJK66_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEZ7578606.1"
                     /translation="MQYRIRRENEAPEAKNAGETLWERDMMREVLLSAYRDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            14333..14429
                     /locus_tag="WJK66_000872"
                     /pseudo
     CDS             14333..14429
                     /locus_tag="WJK66_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         3080 a         3650 c         4334 g         3365 t
ORIGIN      
        1 gcgggaatga cggaaagtgg cgggaatgac ggttcgggca ttccttaaat tacctgtgta
       61 tcgttgtaaa tcttagagat ggcggaatat agcggattaa caaaaaccag tacagcgttg
      121 ccttgccttg gctcaaagag aacgattctc taaggtgctc aagcaccgag tgaatcggtt
      181 ccgtactatt tgtactgtct gcggcttcgt cgccttatcc tgatttttgt taattcacta
      241 tatcgattct gttcccttgc aggcggacgg cggcggcgga cggtggaaaa gcaggttttc
      301 aaatgtccgg tttggcggcg cgccgtctga aaaccgctta ttttcctttg ctgtttccgg
      361 cggagtcttt tttgtccgaa ccggagaagc ggtcgaaacg tatggtgtat gtcagctcgc
      421 cgcccgacga acggctgccg atacgggcaa ccgcctgtat ggcgcgggtc agccggtaaa
      481 tcagtttgac ggactgttcc gcgctggaga tgctgtattc gtagccgatg tagagtttgc
      541 cggtcagttg tttgccgacg gtcagcacct gttcggcggg gttgagttcg ccggtttgcg
      601 cgttgcggct gcgcttgctg gtaaagccca aatcatccac cagcccgatg cggtcgttga
      661 tttgcccggc aagcagcgcg ccggcggctg cggacagggc ggcattgtcg ccgctgctgc
      721 cactgccggc gcggttgagg atgagccagg agagcttgtc tttttcactc atcggttcgt
      781 ttgccgtcag cgtaatgcgc ggactgttga ggctgcccaa tatttccacg cccgcaccga
      841 cgggggaaag gcggcgttcg gcgcggatgt tgaggttggg gtcgttgagc gggccgacaa
      901 aggagaccgt gcctttggta atgtccaaat cctgcccgta tgccttataa cgccctttga
      961 tgacgcggac cgtgcccacg ccccgcacgc ttccgcccga ttgggcggtc agggtcagtt
     1021 tgccgcctat ggtaacgtcc gcgccgtagc cggcgaagcg gatgccgtca ttgaggtcta
     1081 aagtcaggtt catattgacg gggagcggtg ccgccgcctc ttttttgact tcgcctaata
     1141 cgacgacatc gtcgccgacg gacggcatcg aggatttttg cgaaccgaac agcccctgat
     1201 ccgttttaat catcccggta acggatatgc ctttttgcgg cgaatagcgc aggcgggtgt
     1261 tgccggaaac cgtcaggcgg cggttggggc gggacaggat gcggtatttg tcgaacaccg
     1321 cgccgatatc gacatcgggt ccgctgtttt ccataccgac cgtaccggag agttccgccg
     1381 tcccttcgtg ccggaatttc aggctgtcga ttacccattt cctgcccgcg atatgcgaac
     1441 gcagcgagcc gttgtccaag atgatgcctt gggtttggtt gcggtaatag agtttgtcgc
     1501 cgttgatgct gccgcccaag tgcggatcgg cgatgctgcc gccgagggtt acggcggcat
     1561 tcaggctgcc tttgacggtt tgtccgaccg gtaggaagtt gcggaatact tcggcatcgg
     1621 caacggtcag gttgagcctg ccgcccaaag gcgcggtatc gaaagagcgg ctttgcccga
     1681 cggtgatgtt gccgttgatt ttcccgtagt tgctgctgcc gttgacggcg gcattgacgg
     1741 acggagagcc gacccgtccg ccgatttgcg cggcggcatt caggctgccg gtaatgtttt
     1801 gcgcggcggc gggcagaaag ggcttcaatg tgcccaagtc gggaagggag gcggtaatcc
     1861 tgccgccgag cggtgcattt gccatattgc cgccgaaggc gttgccgatg tccaaatcgg
     1921 cgttaatccg cccgaaacgc gcgccgccgt caagcaggat tccgatacgg tcgttttgaa
     1981 agcgcgtttt cagggaaaat gcgttcaaac ccaaagcctg cccgccgggc aatacggcat
     2041 cgccgctttg ccggctgata ttgaggtagc cgcgcgcgtt tcgcccgtag gcgacatccc
     2101 agtcgccgtt taaaaccaga ttgtgttcga agggcggttt gaagaaattg tgcaactcgg
     2161 cgatatgcag accgtgtgcg ccgccttttg ccgatatgcc ggttttttta tcccaagaaa
     2221 agtgttgcag gttgaggctg ccgcccattg cctgccaatt tgccgcactt gccgccacgc
     2281 gttccgcacc ggcttcgagc gtcatacggt tttgcagctt gaggttgaat gcgccgccga
     2341 tgtcgaggat gccgatgctg cctttccatc gggtaagttc cctgttgatg ccgcctgaag
     2401 cgtccaaatc gaatttgaac ggtttgccat ccagcgtcat ggcggcgtgt gtgcggatgc
     2461 ggtgctgcac gcccgtgccg tccagcatca ggtcggcggt atcgacaacc gccgctccgc
     2521 ccgacagcga aaggcggctg cctttaatgt cggcgcgtat cgggcggctt gtgtcgggcg
     2581 aacctttgag cgtgaaatcg agcgaacgga tgtctgccgc cttgccgatg tgcaggttgc
     2641 gcgccgcgcc ggaaaggtcg gtttcaaagg ttcggatgcc accgtccaaa tcgccggaaa
     2701 ggtgtccgcg tacatttaaa gaccccgcga gtccgaaacc gaaacgggat aaatcgggtg
     2761 cggtgatatt gaggttaagc cggtcgcctt ttttgccgaa gccgccgtct gttttaataa
     2821 tgttccgccc cagccgcaaa tcgacggcgg cacgcggaag gtggcgggac tcgtaaacaa
     2881 tgtcggcact gccggcaatc ggtacgccgt tgaacgtgcc gggtaaaaac cgcattttgc
     2941 ctgtgaattt ttcttttgcc agttcgccgg caaggtttat tgagccgttg atattgcctg
     3001 ccggaagttg cggatcgatg cgcgaagggt cgaatgcgcg ggaacggatg tccagcttga
     3061 gcaggcggtc tttaaacagc tcgagatagc cttgcgcggt caggctgcct tgcccggcgg
     3121 cgatgttgac ggtgtcgagc accagtttcc gctgtccgtt tgcggggtcg cttgcaatgg
     3181 cgaggctgcc gtccgtgcgc gccgtgccga tgccgagttg ccaagagatt ttgggcgagg
     3241 cggtcgtgcc accgatgccg atgctgccgt ccaacctgcc tttgaacgcg gtttgcagta
     3301 cgtcttccgc gccgacggag ttgatgccta tatttaaatc gaggatgtct ttttcggtgt
     3361 cgattttgcc cgacagcctg atgccgcccc gtccgagcag ggcgacggac gtattgccga
     3421 tatgcaccgt gccgtcctgc cggatgacaa agctgcctaa aacctgacgg acggggatgc
     3481 cgttgcggtc ggcaaagccg gctttggtgt tttccaaatc gagcgagcct tccagcgcga
     3541 tgccgtctga aaacgacggg atggcggtca ggtcgaaatt cagcccggca tcgggcaggg
     3601 aaggcacgaa ggcggacgga ttgatgttga accctttgac cagtacttct tccaatgttt
     3661 tatccaatga ttcggcaaac gggtggatga cggattttcc cgagaggcgg atattgccgc
     3721 cgtcgatcgc cagttcggcg cgcacatcct tcaggctgcc gctcagccga gccgtactgt
     3781 gtatggtttt gccttcgagt ccgcctttgg tgtaaatggc ggtatcgagg gcaaacggtt
     3841 ttttcaagcc gaccgaggct gaccccgacg aactgctcca cggcgtgtcg gcagccttca
     3901 ggtcgaggcg gtgtcctttg cggtcgtaac ggtatgaagc atccagccgt tcgagataga
     3961 cggtttgttt gtcaaaggct ttgcccatgc tgattttgcc cgtctcgaag cggtcgagat
     4021 agacggcggc aggcaggtct atgctgtcgg gaaggctgag cggcgggcgt tcttctttag
     4081 gcggagtcgg tttggtaacg atggcgatgt cgccggcgga aatttcggta atgtgcaggc
     4141 tgcggcgcat cagttcggac ggtttccacg cgaagcggaa gcggctgatt ttaaggtctg
     4201 ccccctcggt ttctatcgac cagttgtcgc cgtcgaagcc gtcgagcagc gtgcctttga
     4261 ggttttggga ggaaatgttt acgccgaacc aagacgggat ttggtacagc ccgaagcgca
     4321 aacccgcttc cgtgccggcg agccagccga ggaaacatac tgccaaaatc agaacagaca
     4381 gcagtgccgc cgacagcttc agcagcgggc ggcgtttttt tgccggcggg gtagggcggt
     4441 gttcagacgg cattttgcgc gtgccgtttt cggtcggatc ggtatctgtc ggtgcggtat
     4501 cggtcataat gtctgtttca aaatggtttg ctgcaatgcc gtctgaagcg gccgtatcgg
     4561 tttagaagcg cgttcccaag ctgatgtgcc agcggatttt cttgtcgctg tgcccgtagg
     4621 cgatgtcgaa ggaaaacggc gcgagcgggc tgaaccagcg cacgcccagt cccgaaccgt
     4681 gtttcagctt catacgtttg aaattggcgg cggcgtcgcc catatcgtgg aacaccgcgc
     4741 cggaaagggt gcgcgtaaac ggcagttggt attccaggct gcccaccaag agggcgcgtt
     4801 cgggcaggac cgatccgttc gggccggcaa gcccgatgct gtcgagttcg taaccgcgca
     4861 cggaagacgc gccgccgctg cggaacatca gccctgaagg aacgttggca ttgtcgcggg
     4921 caacggtgta acccgcttgt ccgcgtatga tgaacgtgcc gagttttttg ttttcgggcg
     4981 tgaagaaata acctgcgcgg gcagaggtgc ggatcagcgc ggtggaggac aggaatgcgc
     5041 ccaaagtcgt accgattttg ccgtcgaggt aatggccgtt ttcgggatgc agcacgttgt
     5101 tgagcagctg gcgtttccaa gaggcggtca gcatcgtggc gtggctgttg cccaaatcga
     5161 tatccgagcc ggggattttc cggccttctg cgagaaactc cgcccccagc ctggcatcga
     5221 tgcccgcgcg gtcgcgcaca taccagatgc cgccggagaa ggcgcgtttt tcgaggtttt
     5281 gggtggtcga acggttgtag gaaacgttgc ttgtccagta gttgccccga tagttgcgcg
     5341 gctggctgat gccggcggca agcgtggttt cgtatttgtc catatcccag acgaccgaac
     5401 cgatatagcc tttgttgaag aggttgtaat agtcgtaggc gattttgccg cccaaaccgt
     5461 attccgaatc gaggcggatg ccggtttcga gcttgtggcg tttgacctcg gttacgctga
     5521 ctttgacggg gacgcggtcg ccttggaggc ggtcgaagtc ggcttgtacg gacgcgccgg
     5581 aataatgccc gttttgttcg agcgcctgtt ggaagtcgag cagcaggtcg aggtcgtagg
     5641 gcgtgcccgg ttggaagcgc gccaagccgg agacgatttg ttcggggtaa cgctgcgtgc
     5701 cggtaatttc aaagtcgccg aaggcgatgg ggcggccgct gtccacgacg acgttcaaat
     5761 cggcggtggc ggtatcgggg ttgacggccg cccgggtgtt gccgagcttg gcaagcgggt
     5821 aggctttgcg cgttaccgcg ccgaggacgg aagttttgct gttttcccaa ctgtcctgat
     5881 cgaaatcact gcctaccggc tgctgccagt tttccagcgc gttgcggtag tattcggcga
     5941 ggttgccgtc tgaaaggatg tcgccgagga tggcgacgcc gacgttggcg attttggtgc
     6001 gcgggcccgg tgtgatgtgt accgtataag ctccgtcttt ttccgtcagg ctgactttgc
     6061 tgctgaaata gcctttgctg tggagcattg ttttaacgtt gtccggtgct tcttcggcga
     6121 ggaagcccgt ctgttccttg tccaatactt cttcctgctg ctgcgtgatg agcggcaggt
     6181 gttcttcgac catatctttg atttcactat cctgcgtgtc gatgcggacg gggaatttgg
     6241 gttttaattt aactgattcg gtgtcggggc ttttgttttt gaacaatgcg aaacccgccg
     6301 ccttgttttc ggaaaggtcg gcggcaggcg cgtatgcgtg cggaaagaaa aataaagccg
     6361 gcaggagcag ggcggtcggt ttaatcatca tggtacgggt gtcgtgcatc ggtgtggcgg
     6421 ttttcgattc taacactgtt cggacagggt ttcgaacggc cgtttttgtg ttgttcgtac
     6481 ggggcgggac gcttcagacg gcatactgcc gccggtattt cggaggttgc ggcccgccgg
     6541 aaatgtatct gcttgtttta aggtattttg tttctaatcc gcatatggaa aacaggctgc
     6601 gtttcggaat agtgtcggaa aataaacggg attaaataaa tttattttcg ttcaataaat
     6661 taaaccgtcg cgccgaatga taaagcaaag cctgtccggc actgcatagg gcaaatcaac
     6721 gcaaccttca tagtggatta acaaaaacca gtacggcgtt ggctcgcctt agctcaaaga
     6781 gaacgattct ttaaggtgct gaagcaccaa gtgaatcggt tctgtactat ttgtactgtc
     6841 tgcggcttcg tcgccttgtc atgatttttg ttaatccact ataaaacaca gccgaaaaat
     6901 aaaaaaaaca cagagaatga gttttctttt aaaatacaac attttttaac atatcacaac
     6961 ataaggaaga attatggcgt tcggcaaatc cttatttcat gcgatcggcc gcgtcagcct
     7021 ggtcaggcag attgccgccg gtttggcgtt gggcatcgta atcggttcgg tttccccgca
     7081 actgggcttg gcggcaggct tgttcggcag cctgtttgtc ggcgcgctca aagcggtcgc
     7141 gccggttttg gtatttattt tggtggcggc cacaatcgcg cagcaccaaa aaggcaacaa
     7201 ggcgcatatc aggccgatta tcgtccttta cctcatcggt acgttttccg cagccctgac
     7261 cgccgtcatc gccggtatgg ttttcccgac gcacattgtt ttggcgggcg cgggcgatgt
     7321 gtccgccgcg ccgccttccg gcattgtgga agtattgaaa tcgctgctga tgaacctggt
     7381 cgccaacccg attaacgcga ttgccaatgc caactatatc ggcattttgg cttgggcttt
     7441 ggttttgggc gcggcgttgc ggaatcacgg ttcggacgtt acgcggcagg tcgttgccga
     7501 tttggcggaa gcggtttcca ccgtcgtgaa atggattatc cgttttgccc ctttgggcat
     7561 tttcgggctg gtgtcgtcca caatcgcgga gacgggtttc ggcgcgctgg cgggttatgc
     7621 gaagctgctt gcggtactgt tgggctgtat ggcgtttatc gcgctggtgg tcaatcccgc
     7681 catcgtgtgg tggaaaatcc gccgcaaccc ttatccgctg gtgtttacct gcctgcgcga
     7741 aagcggcgtg tatgccttct ttacccgttc ttccgccgcc aatatccccg tgaatatggc
     7801 tttggcgaaa aaactggggc tgcacgaaga cacttattcg atttccatcc cgttgggggc
     7861 gaccgtcaat atgggcggcg cggcgattac gattaccgtt ttggctatgg cggcggcgta
     7921 cacccaaggc atacaggttg attttgccac cgcgctgctg ttgagcctgg tggcgacggt
     7981 cagcgcgtgc ggcgcgtccg gtgtggcggg cggttcgctg ctgctgattc cgctggcgtg
     8041 cagcctgttc ggcatcagca acgatgtcgc tatgcaggtg gtcgccgtcg gctttatcat
     8101 cggcgtgatt caggattcgg cggaaacggc gttgaattct tcaaccgatg ttttgtttac
     8161 cgccgccgcc gatttgggca gacagagaaa ccgggcggaa taaaccaagc tatgccgtct
     8221 gaagcccttt tggcgttcag acggcatatt tttacaattt tatgggcata tggcggaaac
     8281 aataccggct gccttgaata tgaaggagat gatgatgttt aaacgcagtg tgattgcaat
     8341 ggcttgtatt gttgcccttt cagcctgtgg gggcggcggt ggcggatcgc ccgatgttaa
     8401 gtcggcggac acgctgtcaa aacctgccgc ccctgttgtt actgaagatg tcggggaaga
     8461 ggtgctgccg aaagaaaaga aagatgagga ggcggtgagt ggtgcgccgc aagccgatac
     8521 gcaggacgca accgccggaa aaggcggtca agatatggcg gcagtttcgg cagaaaatac
     8581 aggcaatggc ggtgcggcaa caacggataa tcccgaaaat aaagacgagg gaccgcaaaa
     8641 tgatatgccg caaaatgccg ccgatacaga tagttcgaca ccgaatcaca cccctgcacc
     8701 gaatatgcca accagagata tgggaaacca agcaccggat gccggggaat cggcacaacc
     8761 ggcaaaccaa ccggatatgg caaatgcggc ggacggaatg cagggggacg atccgtcggc
     8821 aggggaaaat gccggcaata cggcagatca agctgcaaat caagctgaaa acaatcaagt
     8881 cggcggctct caaaatcctg cctcttcaac caatcctaac gccacgaatg gcggcagcga
     8941 ttttggaagg ataaatgtag ctaatggcat caagcttgac agcggttcgg aaaatgtaac
     9001 gttgacacat tgtaaagaca aagtatgcga tagagatttc ttagatgaag aagcaccacc
     9061 aaaatcagaa tttgaaaaat taagtgatga agaaaaaatt aataaatata aaaaagacga
     9121 gcaacgagag aattttgtcg gtttggttgc tgacagggta gaaaagaatg gaactaacaa
     9181 atatgtcatc atttataaag acaagtccgc ttcatcttca tctgcgcgat tcaggcgttc
     9241 tgcacggtcg aggcggtcgc ttccggccga gatgccgctg attcccgtca atcaggcgga
     9301 tacgctgatt gtcgatgggg aagcggtcag cctgacgggg cattccggca atatcttcgc
     9361 gcccgaaggg aattaccggt atctgactta cggggcggaa aaattgtccg gcggatcgta
     9421 tgccctcagt gtgcaaggcg aaccggcaaa aggcgaaatg cttgcgggca cggccgtgta
     9481 caacggcgaa gtgctgcatt tccatatgga aaacggccgt ccgtccccgt ccggaggcag
     9541 gtttgccgca aaagtcgatt tcggcagcaa atctgtggac ggcattatcg acagcggcga
     9601 tgatttgcat atgggtacgc aaaaattcaa agccgttatc gatggaaacg gctttaaggg
     9661 gacttggacg gaaaatggcg gcggggatgt ttccggaagg ttttacggcc cggccggcga
     9721 agaagtggcg ggaaaataca gctatcgccc gacagatgcg gaaaagggcg gattcggcgt
     9781 gtttgccggc aaaaaagagc aggattgatg ctgatgccgc ctgaagcggg ggcgttcaga
     9841 cggcattgtg atgtttgggg tggggtccgg ttcaagtccg catcagttga taaacccgtc
     9901 gtctttcatt ttcttttcgg aaaaatactg gccgaatacc agcagggcag cgaggatgat
     9961 gacaaacagt cccgccatag tcagagtgat tttgaaaaac aattccgctt tgagaaaaga
    10021 aaaccagagt tgcacaatca gcagcagcac ccagccggca aacagtccga gacaggcata
    10081 aacgccgagc ttaaacacga tcccgctcca aaatgacgac gtaggcctcg cgtttgaaaa
    10141 acagcagcat acggcgcaaa tctttttcca tcgttacgac ccgccagcct tcgggggatt
    10201 tgtttgttga ggaacgcgct gaaattgacg gggttgacct ttgccgcgcc gaaaaacagg
    10261 ctggacaaca ggctttcctg ataaatgacg actttgtatt ctttcatagg attcctttta
    10321 acataaggga tcatcttttc agacgacccc ttaagttgct aatgatgtaa aacagcaaac
    10381 cttattgctt attgcccaac tgcggcaata ccgaaccttc gcccagcgag agcaaaccgc
    10441 tttgcgagta gatacccagt ttgacgcggg tgtcggtgat gtcgaggttg cgcatcgtga
    10501 gctgtccgat gcggtcgagc ggagtgaacg cagcgtcttc gactttttcc atactcaggc
    10561 gttcaggttg gtaggtcagg ttgggcgatt cggtgttcag aattgagtag tcgttgccgc
    10621 gccgcagttc gagggtaact tcgccggtaa cggctttggc aacccagcgt tgggcggttt
    10681 cgcgcaacat cagggcttgg ctgtcaaacc agcggccttg gtagagcaaa cggccgaggc
    10741 gcaggccgtt gatgcggtat tgttcgatgg tgtcttcgtt gtggatgccg gtcaccaagc
    10801 gttcgtaggc gatgtggaac aacgccattc ccggggcttc gtagatgccg cgcgatttgg
    10861 cttcgatgat gcggttttcg atttggtcgc tcatgcctaa gccgtggcgg ccgccgatgc
    10921 ggttggcttc gaggaagagt tcgacggggt cggcgtattc tttgccgttt aatgcgacgg
    10981 gcacgccttc ttcaaagcgc acgctgactt cttcgggttc gatttcgacg ttttcgtccc
    11041 aaaaggcaac gcccataatg ggtttgacga ttttgatgcc cgagttcaaa aattccaagt
    11101 ctttggcttc gtgggtcgcg ccgagcatat tggaatcggt ggagtaggct ttttcaaccg
    11161 acattttgta gttgaagccg ttggcaatca gaaattcgct catttcgtga cggccgccga
    11221 gttcgtcgat aaattgctgg tcgagccagg gtttgtagat tttcagcgcg ggattggtga
    11281 gcaaaccgta gcggtagaaa cgctcgatgt cgttgccttt gtaggtgctg ccgtcgcccc
    11341 agatgtttac gtcgtcttct ttcattgcgg aaacaagcat cgtgccggtt acggcgcggc
    11401 ccagaggcgt ggtgttgaaa taggcgatgc cgccggtgga aacgtggaac gcgccgcatt
    11461 ggatggcggc gatgccttcg tgtgccaact gcgcgcggca gtcgattaag cgggcgtttt
    11521 ccgcaccgta ttccatcgct tttttgggaa tggcgttgta gtcgtcttca tcgggctgac
    11581 cgaggttggc agtgtaggca taaggcagtg cgcctttgag tttcatccac aacagcgcgg
    11641 cggaggtatc aagtccgccg gagaaggcga tgccgacttt ctgaccgacg gggaggtttt
    11701 gtaaaatagt atggttttgg ttcatgaaaa aatcctcggt agcttgaaaa atcaaagcca
    11761 ttatcttata aataggaaat attgtcagcc atcatgccgt ctgaacgggt attccgccgt
    11821 ttcaggcggt atttaaaacc gaaaacatta tgaacgcgtt caaccttatc atcatcggcg
    11881 acgaaatctt acacggcagc cgccaagaca agcatttcgt ctttttcaaa tccctgctcg
    11941 aatccaaagg gctgaagctc aatcaggtgc aatacctgcc cgacgaacct gatttgttgg
    12001 tcaaacaact gcgccgcagc ttttcagacg gcatgccaac tttcgttacc ggcggtatcg
    12061 gcgccacgcc cgacgaccac acccgccaag ccgcagccgc tgctttggat ttgcccgtcg
    12121 tccgccacgc gcaagccgcc gggtttatcg aaggcgtgac ccttaaacgc ggtgagccgc
    12181 tcgattcgcc cgaacacgcc caacgcctga agatggcgga ttttccccaa ggcgcggaat
    12241 tggtgcctaa tccgtttaat aatatcgccg gattttccat tcgcgagcat tatttcttcc
    12301 ctggttttcc tgtgatggcg cacccgatgg cggaatgggt tttggatact tattacgccg
    12361 gccgcttcaa ccaaacccaa cgcggcagcc gcagcgtgta tgtgttcgag cagcccgaat
    12421 cgcgcattac gccgattatg gagcatatcg agcaaactta tctcggggtg cgttcgtaca
    12481 gcctgcccag cgtcggttgg acgcattcag acggcacgca ggtcaaaccg cacattgagt
    12541 tcggcatcaa agccgaagga gaagccgtca accttttgga cgcggcgtgg gcggaagtgt
    12601 tgcacagctt ggaaggattg ggagcagagc tgaaaaatcg ggtaaactga aaaacgtttg
    12661 cccaaatcgg acgatattta aggaaaacca tgcaataccg aatcagaaga gaaaacgaag
    12721 cacccgaagc aaaaaatgcg ggggaaacct tatgggagcg cgatatgatg cgcgaagtgc
    12781 tgctgtccgc ctatcgggac aggcgcaggg agcggatgtg gaaaaacatc tggcgcgccg
    12841 tcagcaccct gattctggtt gccctgattg caggcatttt ccgaaaagac gaagccgcat
    12901 tgcagttggc gggcaatacg ccgcataccg cagtcgtcaa cctgtacggc gaaatcggca
    12961 acggtgtaga agaccaggtc aaaaaactca aagacggtat ggaggccgcc tacaaaaacc
    13021 cgcaggcaaa agccatcgtc atccgcgcca acagccccgg cggttcgccc gtcgtctcca
    13081 acaccgcttt tgaagaaata cgccgtctga aggcgcagca ccccggcatt cccgtttatc
    13141 tcgtggcgga agatatgtgc gcgtccggct gctactacat cgcggcggcg gcggacaaaa
    13201 tctatgccga cccgtccagc atcgtcggca gcatcggcgt tatcggcagc agtttcgacg
    13261 cgaccggcct gatggaaaaa atcggcgtga aacgcagggt taaaattgcg ggcagcaaca
    13321 aaggcatggg cgatccgttt tcgcccgaaa cgcccgaaca gtcgaaaatc tgggaagaaa
    13381 tgctgaccgg catacacggc gaattcatca aagccgtcaa aaccggacgg ggcggccgcc
    13441 tcaaattccg acaatatccc gacgtgttca gcggtcgggt gtacacgggt gcggatgcgc
    13501 tgaaagtcgg gctggtggac ggactcggca atatttacag cgtggcgcga gacgtggtca
    13561 aagcccccga tgtggtggat tacactccga aggacgattt cggcagaatc ttgggcagac
    13621 gcttcggcgc agaactcaaa gcaagcgtgc gcgaagccct gcaggccgtc cggtagcgtt
    13681 gtttacagta tttctaaggg aataccaaaa taaaaatata cacaaaaaca ctcacctccc
    13741 cagtatgctg ccactaagtc agccgcgtca ctatttaccg tgcacttaaa gccgtaaaag
    13801 gcaggttgtc cccgggaggt tttgtgcatc ctgataccga tcggttgttg ttgctcaaag
    13861 gacagaaggc cgctgataaa cgagattacc tgtttgtcgc tattgacgat ttttatactc
    13921 tgccattttg ccagacaaaa ccgcagacag tgctgccaag tttctgaccg aacatctggc
    13981 cgacccctgc ttgtacctga ttgagtacgt ttactctgac aatgataggt aatataaaga
    14041 gccgtccaac atgctttcgg tgcagtttgt tatgataatg ggattggttg gaggcttgcc
    14101 cgatttgctt gtccgcagac caacggtaag gcggagcggg ttatccgtac cttgatggag
    14161 atgtggcatg aggaacagtc gtttgacagt ccggaacatc ggcaaaagga gttgtgctgc
    14221 tttattaatt taggggctgc cctagatcac tagggaaatt caaattaagt tagaattatc
    14281 cctatgagag aaagtcgtct aagccggtat aaacaaaata aactcatttt gttatcgtgg
    14341 ctatgatgta ttggatgcgg gcgaatttag ccatttccgt atcaatcaca gcacacattt
    14401 tgccgaacga caaaaccata ttaatggaa
//
LOCUS       DAWKOG010000039        13821 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.039, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000039 DAWKOG010000000
VERSION     DAWKOG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13821
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.039"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(228..551)
                     /locus_tag="WJK66_000873"
     CDS             complement(228..551)
                     /locus_tag="WJK66_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEZ7578607.1"
                     /translation="MDIVQLGSAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAAEIDG
                     NMMRY"
     gene            complement(633..1289)
                     /locus_tag="WJK66_000874"
     CDS             complement(633..1289)
                     /locus_tag="WJK66_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEZ7578608.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     ISDDIDAEQQNRAKAVLDGLGLDNIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
                     KEQLKDGGRMVVIVGRKPVQRALLITRRGDVFEEKVLFDTLVAHLDDKDADPFGGFQF
                     "
     gene            complement(1391..1894)
                     /locus_tag="WJK66_000875"
     CDS             complement(1391..1894)
                     /locus_tag="WJK66_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEZ7578609.1"
                     /translation="MHALHFSASDKAALYREVLPQIESVVADETDWVANLANTAAVLK
                     EAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGTVVVGDVDA
                     HPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGELAKILEKRF
                     EASRQAV"
     gene            2078..2851
                     /locus_tag="WJK66_000876"
     CDS             2078..2851
                     /locus_tag="WJK66_000876"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEZ7578610.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            2858..3208
                     /gene="secG"
                     /locus_tag="WJK66_000877"
     CDS             2858..3208
                     /gene="secG"
                     /locus_tag="WJK66_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEZ7578611.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            3222..3307
                     /locus_tag="WJK66_000878"
     tRNA            3222..3307
                     /locus_tag="WJK66_000878"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3256..3258,aa:Leu,seq:gag)
     gene            4108..4476
                     /locus_tag="WJK66_000879"
     CDS             4108..4476
                     /locus_tag="WJK66_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEZ7578612.1"
                     /translation="MRIFKNQWIVKFAKKHKINDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLILFKQADRAFFVYAFAKNDRENISDKELDVYRKAAAYYLK
                     YTRAELAALKEDGIITEIES"
     gene            4473..4784
                     /locus_tag="WJK66_000880"
     CDS             4473..4784
                     /locus_tag="WJK66_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEZ7578613.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(5121..5975)
                     /locus_tag="WJK66_000881"
     CDS             complement(5121..5975)
                     /locus_tag="WJK66_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ7578614.1"
                     /translation="MSFLPSFLPSFKRIVCLSAVISILGGCAVVADVYGHDSATMNAA
                     AAEDYMKTVELNKSAGNVDTTSRTARRVQAVFRRMLPYADAANNTGHRFDWKMTVFKN
                     DELNAWAMPGGKMAFYTGIVDKLKLTDDEIAAIMGHEMTHALHEHGKNKVGQQILTNT
                     AAQIGTQIILDKKPDTNPELVGLGMDILGTYGITLPYSRSLEEEADEGGMMLMAQAGY
                     HPAAAVRVWEKMNQENDQNGFIYAITSTHPTNNARIENLKRLLPTVMPVYEQSVRNKG
                     RVNKKRRR"
     gene            complement(6082..6195)
                     /locus_tag="WJK66_000882"
     CDS             complement(6082..6195)
                     /locus_tag="WJK66_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl-tRNA synthetase"
                     /protein_id="HEZ7578615.1"
                     /translation="MRDSDKPAASEGAVKFIEGNPAKKIIAVPGRLASIVV"
     gene            complement(6325..7116)
                     /locus_tag="WJK66_000883"
     CDS             complement(6325..7116)
                     /locus_tag="WJK66_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ7578616.1"
                     /translation="MNLFFDTELGRQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMQR
                     FANNKPVADLFCPNCHEQYELKSKNQKTIGNSVPDGAYRTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPNHIMCSINLAPLPESGKIFLIDDS
                     RIIEPETVLKKWQSNLFLRNQNAERKGWLLAVMKCIDQLPEEFTLSQMYEFEKKLSIQ
                     FPQNNHIRDKIRQQLQILRDQNTIEFIGRGLYKKIDKLPPTSKAF"
     gene            complement(7348..10080)
                     /locus_tag="WJK66_000884"
     CDS             complement(7348..10080)
                     /locus_tag="WJK66_000884"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEZ7578617.1"
                     /translation="MQEQYQPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYSLPKKQVLQRVTIGLSPEQEEAMGFGGGSGC
                     GAGYGDGSGSDSRDLPFMPNKWQAWYGVKDDFMVLINSGELDGMNYQTAFDAVAAKLQ
                     AIGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCEQCGDVPVPADQLPVVLPENVVPDG
                     MGSPLAKMPEFYETTCPCCGGAAKRETDTMDTFMESSWYFFRYMSPKFSDGMVDPAAA
                     KYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEGLVNVDEPFERLLTQGMVVCETYYR
                     ENDKGGKDWINPADVELTFDDKGRPVSAVLKVDGLPVVISGTEKMSKSKNNGVDPQEL
                     INAYGADTARLFMMFAAPPEQSLEWSDSGVEGAHRFLRRLWRTVYEYLKQGEAVNAFA
                     GSQDGLSKELKDLRHKLHSTIAKVSDDYGRRQQFNTAIAAVMELLNQYDKTDTGGEQG
                     RAVAQEVLEAAVRLLWPIVPHICETLWSELNGAKLWEAGWPTVDEAALVKSEIEVMVQ
                     VNGKLRGKITVAADTSKADLEASALATEGAVKFMEGKPAKKIIVVPGRLVNIVV"
     gene            10350..10865
                     /locus_tag="WJK66_000885"
     CDS             10350..10865
                     /locus_tag="WJK66_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ7578618.1"
                     /translation="MKIKQIVKPGLAVLAAGVLSACATKSNVKADGTTDNPVFPKPYS
                     VTLDNKRGTFPTYDELDQMRPGLTKDDIYKILGRPHYDEGMYGVREWDYLFHFHTPGV
                     GIDPENTSGVEDVTTCQYKVIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            10872..11315
                     /locus_tag="WJK66_000886"
     CDS             10872..11315
                     /locus_tag="WJK66_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HEZ7578619.1"
                     /translation="MPFRLFLGILCGLSMVAPVYAQGQPDAVNAYIQKKKVIVNTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            11484..13541
                     /gene="ppk1"
                     /locus_tag="WJK66_000887"
     CDS             11484..13541
                     /gene="ppk1"
                     /locus_tag="WJK66_000887"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEZ7578620.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELARESIHFYRRRNWTGAQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDLQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPTHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPDVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGIITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIIGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWTL
                     LGG"
BASE COUNT         3433 a         3905 c         3382 g         3101 t
ORIGIN      
        1 cagcctgcac attgaaaatg ccgcccaagc aaaccttcag tttgccagcc tcgtcctagc
       61 cctctcccac ggggagaggg gattggggtg caggctgcct ttaaggttca ggcaaatttt
      121 aacttcgttg ataccgcgct ttagcttcgc agaagctgca ctttcagacg accttttgga
      181 acaccacagg tacacgcatt taaggaatgc cgtctgaaat gcctgcctca ataacgcatc
      241 atgttgccgt caatctcggc cgcccatgca tcgatgccgc cctgaaggtt gtacaggttt
      301 tcaaaacccg cgtccgccaa atacatcgcc gtatgcagac tgcggatacc gtgatggcaa
      361 tacaccacaa gcggcacatc atccggcagc tcgttctgcc gcagcggaat cagattcatc
      421 gggatatgca gcgcatttgg cagcgaacaa accgccgttt cttcatccgt acgcacgtcc
      481 aacaaacaaa acatccgccc ttcgtccatc cacgccttca attccgccga cccaagttgc
      541 acaatatcca tcgcaccccc caaaaaaaaa aaccaagcaa aatgccgtct gaagccccaa
      601 acccgctttc agacggcatc gcaatccgaa catcaaaact gaaaaccacc aaacggatcg
      661 gcatccttat catccaaatg cgccaccaag gtatcgaaca gcaccttctc ttcaaacaca
      721 tcgcccctgc gcgtaatcaa aagcgcgcgt tgaacaggct tgcgtcctac gataaccacc
      781 atgcgtccgc catctttcaa ctgttctttc aacacttcag gcacaaggtt taccgcaccg
      841 ccgacataaa ccgcatcaaa cggcgcacct gcggaaagtt cggtcaaccc gttgttttgc
      901 acataatcga tattgtccaa acccaagccg tccaacaccg ctttggcgcg gttttgctgt
      961 tccgcatcga tatcgtccga aattaccctg cccgccaatt ttgccaacag cgcggtcgca
     1021 tagcccgaac ccgtgccgat ttccaaaacc gtatcgtttt tcgtcagctt caagccctgc
     1081 gccagccgcg ccacgacttt cggctccagc atcttatgac cgttggcaag cggcagcgcc
     1141 atatccgcat acgccaaacc ctgcaagtcc tcatcgacaa aaagctcgcg cggaatctcc
     1201 gccaaagcgt ccaaaacgtc aaaatccaat acatcccaag gacggatttg ctgttcgacc
     1261 atattgaacc gcgctttttc aaaatccatt tagtgctccg tcaaataatt gttccaacaa
     1321 tagcaggcat tataaaacca cactcccgcc gcgccaactt cggcacgcgc cgcccgcccc
     1381 tttccctcac tcaaaccgcc tgacgcgaag cctcaaaccg cttctccaaa atcttcgcca
     1441 gctcgcccaa atacaaagca tctgtctcat caaactgcgc caaatgttcg ctgtccgcgt
     1501 ccaacacgcc gatacagcgg ccgtctgaaa acagcggcac gacaatctcc gaacgcgaca
     1561 aagacgaaca ggcaatatgg tcgggatgcg cgtccacatc cccgacgacc accgtcccgc
     1621 ccttcgccca agcctgaccg cacaccccgc gaccgaacgg aatccgcgta cacgccaaag
     1681 gcccctgaaa cggtgccaaa accaattcgt ccgaacgcgt atcgaccaaa taaaaaccca
     1741 cccaaaacca accgaacgcc tccttcaaaa ccgccgccgt gttcgccaaa ttcgccaccc
     1801 aatccgtctc gtccgccacc acagactcaa tctgcggcaa cacctcccga taaagcgcgg
     1861 ccttgtccga agccgaaaaa tgaagcgcgt gcatcacatc tcctatagtt gcatacatat
     1921 caggcggcca ttataaaaca acctgcccga aacaacattc caaaccgccc ccgcagccgc
     1981 ccgccccaag ttgcgaaccc gccgcatatc cactaaactt cacattgcaa cgcgccacac
     2041 gcggcagaca gaaaaaacac gacacggagc aaaaaagatg tatcaccaaa tcggaatgtg
     2101 ggatcaaaaa tgggtcatcg gcaactggaa aatgaacggc cggctccaga acaacaattc
     2161 gctcatgcac cgcttccgca tcctccccac cgccgaacgc gtcctcatcg gactcgccgc
     2221 cccgaccgtt tacctgctgc aactgcacaa cgccatgcaa atcgtcttaa acaaccgcat
     2281 cctcacctgc gcccaagacg tgagccgctt ccccgacaac ggcgcgtaca ccggcgaagt
     2341 gtccgccgaa atgctcgccg acaccggcac agacatcgtc ctcatcggac actccgaacg
     2401 cagcctttat ttcggcgaaa aaaacgaaat ccaacgccgc aaaatggaaa acgtcctcaa
     2461 cgtcggactc atcccgttat tgtgcgtcgg cgaaagcctc gaagagcgcg aagccggcaa
     2521 agaacacgaa gtcatcgccc atcagctttc catcctgcaa gggctggata ccaaaaacat
     2581 cgccgtcgcc tacgaacccg tctgggcgat cggcaccggc aaagtcgcca ccgtcgaaca
     2641 gattgccgat atgcacgcat tcatctacaa agaaatcttg tctttgtgcg gaagcgatgt
     2701 taaaatccgc gtcctttacg gcggaagtgt gaaagcggac aacgcggccg acatcttcgc
     2761 agtaccttat gtggacggcg cactcgtcgg cggcgcgtca ttgtcgtacg actcctttac
     2821 cgccatcatc agtgccgcac aaaatgcgta gaaaaatatg gaagccttca aaaccctaat
     2881 ttggattgtt aatataattt ccgctttggc cgtcatcgtg ttagtattgc tccaacacgg
     2941 caaaggcgcg gatgccggcg cgactttcgg atcgggaagc ggcagcgcgc aaggcgtatt
     3001 cggctctgcc ggcaacgcta acttcctcag ccgctcgacc gccgttgcag caacattttt
     3061 ctttgcaacc tgcatggcta tggtgtatat tcacacccac acgacaaaac acggtttgga
     3121 cttcagcaac gtacaacaaa ctcagcaagc acccaaaccc gtaagcaata ccgaaccttc
     3181 tgcccctgtt cctcagcagc agaaataaca gtttttcaaa tgccgacatg gtgaaattgg
     3241 tagacacgct atcttgaggg ggtagtggcc gtaggctgtg cgagttcaaa tctcgctgtc
     3301 ggcaccaaca cacaaaacgc ttagaaattc taagcgtttt tttcgtttaa tcctgcctga
     3361 tgccatcaac caatcccaat caatacaagg ttttcaggct gattgttatc ctgccgcccc
     3421 cccttcctga ccgtacaatc ccgtccaatc cgccctaatt gaactaacct gattttttac
     3481 ggcatccgaa aataccgcga aaaatacgtg aaaataaagc tgaacgacca ccaaatcagg
     3541 aatgccaagc gggaaatagc ttgcggggaa tactgacgca ccgtagggaa tgagggggaa
     3601 accgtccaag atgcagagcg gttttttttg ggtttttgga aaaaacctat actatagtgg
     3661 attaaattta aactggtacg gcgttgcctc gccttgccgt accggtttaa atttaattca
     3721 ctataaaata agaaaaagat aaaaaattgg taacaaatgc ggtaacaaat ggtaacgaat
     3781 cggtaacaac ttttggggtt ttccggtttt tcaccgtctt ggcagtggga gcgtagcgga
     3841 atgaaaagcc aaaacgcacg gaaccgcgcc tattttgagc aggaatggcg gttaaaccac
     3901 ttggttatat atgggggcat aggaagacag cgaaacgcgc taaaaacgca atttgagcca
     3961 ttaaaagcga ttgattaaaa aataatcagg ttagccgcca gtgtttcagg cggcataaac
     4021 ggagaaattg cggggcataa aaaaggcagc ttgccgtgtt gtctgtctct ggtataattc
     4081 caagtatcac taatcaacgg ctacacaatg cggatattca aaaaccaatg gatagtgaaa
     4141 tttgccaaga agcacaaaat caacgattcc gagctgctgg aagcggtaga gcgggcggat
     4201 aacgggctga tagacgcaga tttaggcggc ggtgtgatta agcagcgcat agcaaggcaa
     4261 ggacaaggca gaagcggcgg ctatcgcagc ctgatattgt tcaaacaggc agatagggca
     4321 tttttcgttt acgcctttgc caagaacgac agggaaaaca tttcggataa agaactggac
     4381 gtttaccgaa aagccgccgc atattatctg aaatacacgc gggcagagct ggcggctttg
     4441 aaagaagacg gcattatcac ggagatagaa tcatgaaata caaaagcgag gcattagccg
     4501 ccattcatga aatgatggaa ggggcttaca acatcggcgc aatcgacaaa aagactatgc
     4561 gcggctttga caagtcatgc ctgaccgaaa tcaaaccgtt gagcggcgga gacatcaagg
     4621 caatcaggga gaaggaggca ctatcgcaag ccgctttcgc catctatctc aacgtgggaa
     4681 aaaatcacgt ttcggcttgg gagcggggcg ttaaaaagcc gagcggcgcg gcgttgaagc
     4741 tgctgaccat cgtcaaaaac aagggtatcg aagccattgc gtagccgact tggcaaacgg
     4801 caaaatcagc aagttcacaa tagacgcgct gctgaatatg cctgccaaaa caggcaagac
     4861 cgccgaactg aatatcaggg cgtagccgca taaatgcccc gaccgcatca aaccaagccg
     4921 aaacggcggc ggtgcagacg gcatagcccg acagcaaggc acggggattt ttttgcgccc
     4981 gttgcagggg gattggattt aagcggcgcg gacttgaagg caaaacgggc ggggcgcaga
     5041 actgtttaaa tgccgtctga atctcaaacg gattcagacg gcattttgga acaacggctc
     5101 aaattctcga tccccttccc ttaacgccga cgttttttat taacgcgccc cttatttctg
     5161 acactttgct cataaaccgg cataacggtc ggcaacaacc gttttagatt ttctatacgg
     5221 gcattgtttg tcggatgagt agaggtaata gcataaataa agccgttttg gtcgttttcc
     5281 tgattcattt tttcccaaac cctgacagcg gccgccggat gatagcctgc ctgcgccatc
     5341 aacatcattc ccccctcatc ggcttcttct tccaagctgc ggctataagg caaggtaata
     5401 ccgtacgttc ccaaaatatc catacccaat ccgaccaatt ccggattagt atccggtttt
     5461 ttgtctaata taatctgcgt gcctatctgc gccgccgtat tggtcaagat ttgctgcccg
     5521 accttatttt taccgtgttc atgcagggcg tgcgtcattt catgtcccat aatggcggca
     5581 atttcgtcat cggtcagctt gagtttgtcg actatccccg tataaaacgc cattttcccg
     5641 ccgggcatcg cccacgcgtt cagctcatcg tttttgaaaa ccgtcatttt ccagtcaaac
     5701 ctatggccgg tattatttgc cgcatcggca taaggcaaca tacgtcgaaa tactgcctgc
     5761 accctacggg ctgttctgga tgtggtatcg acattgccgg cagacttgtt taactcaacc
     5821 gttttcatat aatcttcggc agccgcagcg ttcattgtgg cggaatcatg accgtaaaca
     5881 tcagcaacga ccgcacaacc tcccaatatc gaaatgactg ccgacaggca gactatccgt
     5941 ttaaaggaag gaaggaagga aggaaggaag gaaatttcat attaggctta ccccttaaaa
     6001 aattaaattt aaaaaatgcc gtctgaatct aaaacggatt tcggacggca tcttaacatt
     6061 gtttgatgtt tttaaaaaga tttacaccac gatgctcgcc agtctgcccg gtacggcgat
     6121 gattttcttg gcaggattac cttctatgaa tttcactgcg ccttcggagg cggccggttt
     6181 gtcggaatca cgcataaatt gccaataatt ccccgacttt tttacggctg ctgccttttg
     6241 cggcaatatt gcgctgaact tcaactgttt tcagaatagc agaagaataa atatcccttg
     6301 tgaattcagt atcatgattt gaaattaaaa cgccttggaa gtcgggggta atttgtcgat
     6361 ttttttgtaa agtccgcgac caatgaattc gatcgtattt tggtcgcgca aaatttgcaa
     6421 ctgttggcgg attttgtctc tgatatggtt gttttgggga aattggatgg atagtttttt
     6481 ttcaaattca tacatttgtg acaatgtgaa ttcttcgggg agttggtcga tacatttcat
     6541 aacagccaaa agccagcctt tgcgctccgc attttggttg cgtaaaaaca ggttggattg
     6601 ccattttttc agaacggttt cgggttcgat aatgcgggaa tcgtctatta agaatatttt
     6661 tccgctttca ggcaaagggg cgagattgat ggaacacata atgtggttcg gccggttttt
     6721 aataccttta tttctgggaa taatcatatc cggcgtgatg aaatgtttgg gtacaagcac
     6781 caattgccgt atggagtaat ccgctttttt atatgcaaga aagaaaaagt tggggttggt
     6841 atctgaccgg atgcgctcca gcatggtgcg atatgcaccg tcaggcacgc tgttgcctat
     6901 ggttttttga tttttactct ttaattcata ttgctcgtgg caatttgggc aaaagaggtc
     6961 tgcaacaggt ttgttattgg caaatctctg catcggcttg cttccgcaac aggggcagta
     7021 gccgtttttt tccagccaag cctcgctcat tacacggatt ttgtgggttg ctttattttg
     7081 ttgccttccc aattcggtat cgaaaaataa attcatgttt tggattttga gattaagttt
     7141 gagtggatta acaaaaatca ggacaaggcg acgaagccgc agacagcagg gcaaggcggg
     7201 gcaacgccgt accggtttaa agttaatccg ctatatttga ggtctgtcat gcggacaact
     7261 ccatctgcct taaaaaggcc gtctgaaacc ctgtttccaa gtttcagacg gcctttccta
     7321 attacagcag tcaatgccga atcggtttta caccacgatg ttcaccagtc tgcccggtac
     7381 gacgatgatt ttcttggcag gcttgccttc catgaatttc accgcgcctt cggtggcgag
     7441 tgcggaggct tcgaggtcgg ctttggaggt gtcggcggcg acggtgattt tgccgcgcag
     7501 tttgccgttg acttgaacca tgacttcgat ttcggatttg accaaggcgg cttcgtcgac
     7561 tgtcggccag cctgcttccc acagtttcgc accgttcaat tcgctccaca gggtttcgca
     7621 gatgtgcggc acgatgggcc acagcaggcg tacggcggct tccaatactt cttgggcgac
     7681 ggcgcggcct tgttcgccac cggtgtcggt tttgtcgtat tggttgagca gttccatcac
     7741 ggcggcgatg gcggtgttga actgctgacg gcggccgtag tcgtcgctga ctttggcgat
     7801 agtggaatgc agtttgtggc gcaggtcttt gagttcttta gacaaaccgt cttggctgcc
     7861 tgcgaacgcg ttgactgctt cgccttgctt caagtattcg taaacggtac gccacaggcg
     7921 gcgcaggaag cggtgcgcgc cttcgacgcc gctgtcgctc cattcgaggg actgttcggg
     7981 cggtgcggcg aacatcatga acaggcgggc ggtgtctgcg ccgtaggcgt taatcagttc
     8041 ttgcggatcg acgccgttgt ttttggactt ggacattttt tccgtgccgc tgatgacgac
     8101 gggcagtccg tcgactttca ggacggcgga aacagggcgg cctttgtcgt caaaagtcag
     8161 ctcgacatcg gcggggttga tccaatcttt gccgcctttg tcgttttcgc ggtagtaggt
     8221 ttcgcaaacg accatgcctt gcgtcagcag gcgttcaaac ggttcgtcaa cattgaccaa
     8281 accttcgtcg cgcatcagtt tggtgaagaa acgcgcgtac aagaggtgca aaatcgcgtg
     8341 ttcgatgccg ccgatgtatt ggtcgaccgc gccccagtat ttcgcggcgg caggatcgac
     8401 catgccgtct gaaaattttg gcgacatgta gcggaagaag taccagcttg attccatgaa
     8461 ggtgtccatg gtgtcggttt cgcgttttgc cgcgccgccg cagcatgggc aggtggtttc
     8521 gtaaaactcg ggcatttttg ccagcggcga acccatgccg tcgggtacga cgttttcagg
     8581 caaaacgacc ggcaattggt cggcagggac gggtacgtcg ccgcattgtt cgcaatggac
     8641 gatgggaatc gggcagcccc agtagcgttg gcgcgaaatg ccccagtcgc gcaggcggta
     8701 ttgggttttc ggttcgccag ctccgatagc ttgtaattta gcggcgacag catcaaatgc
     8761 tgtctgataa ttcatgccgt ccagttcacc tgagttgatt aagaccatga agtcatcttt
     8821 gactccatac catgcttgcc atttattagg cataaacggc aaatctcttg aatcggaacc
     8881 actaccatca ccataaccag caccacatcc ggaaccgcca ccaaaaccca tagcttcttc
     8941 ttgctctggc gaaagtccga ttgttacgcg ttgtaagacc tgttttttcg gcaggctgta
     9001 tttggcggcg aactcgaaat cgcgttcgtc gtgcgccgga accgccatta ccgcgccgtc
     9061 gccgtagccc cacaatacat agttggcaat ccacacttcc agcttgtcgc cgttgagcgg
     9121 gttgacgacg tagcggccgg tcggcacgcc ttttttctcc atcgtcgcca tatcagcttc
     9181 ggcaaccgaa ccggctttgc attcggcaat aaatgcctgc aattcgggtt tgtcggcggc
     9241 tgcggcggtt gccagcggat gctcggcggc aacggcaaca taagtcgcgc ccatcagcgt
     9301 gtcgggacgg gtggtataaa cttgcaggaa tttcgcgtaa tcgccttcca agccttgttt
     9361 gctgtcgtct gaaacggcga agcgcacggt cataccgcga gatttaccga tccagttgcg
     9421 ctgcatggtt ttgacttgtt ccggccagtg ttccagcttg tccaagtcgt tgagcagttc
     9481 ttcggcgtaa tccgtgattt tgaagtaata catcgggatt tcgcgttttt cgatcaatgc
     9541 gccggaacgc cagccgcgtc cgtcgataac ttgctcgttg gcaaggacgg tttggtcgac
     9601 cgggtcccag tttaccgtgc cgtttttgcg atagacgatg cctttttcaa acagcttggt
     9661 aaacagccat tgttcccagc ggtagtattc gggtttgcag gttgcggttt cgcgcgccca
     9721 gtcaatcgca aaacccaggc ttttgagctg ggttttcatg tattcgatgt tatcgtacgt
     9781 ccaagcggca ggggcgacgt tgtttttcat cgccgcgttt tccgccggca tgccgaacgc
     9841 gtcccaaccc ataggctgca tgacgttgaa gccgtttaaa agtttgaagc ggctcaatac
     9901 atcgccgatg gtgtagttgc gcacatgccc catatgcagc ttgccgctgg gataggggaa
     9961 catggagagg caataatatt tgggtttgga agcgtcttcg gagacgttga aaatacgggc
    10021 gtcgtcccat tttttctgcg ccgcaggctc gatggcggcg ggttgatatt gttcttgcat
    10081 agtcattctg ttttcgctta aaaacgttgg aaaaataaag tcggcatcaa ttataacagg
    10141 ttgccggaag cggcgaatcg gcagattgcc ggcaggatgc gtaaattcgc acgcgcatta
    10201 ttccgtatgc cgtacaaata caccgcgttt attgatacgc acgtttttta tgctaatatt
    10261 acaaaccaaa atcaaatgtt taaaactctc ctgatgcggc tcttccgaac aaaaggcaga
    10321 cgggcatcgg gtaaaagagg attctgcata tgaaaatcaa acaaatcgtc aaaccgggct
    10381 tggcagtatt ggcggcgggc gttctgtctg cctgcgcaac caaaagcaac gtcaaagccg
    10441 acggcacgac cgacaatccg gttttcccga aaccctattc cgtaacgctc gacaacaagc
    10501 gcggcacatt cccgacttat gacgaactgg atcagatgcg ccccggcctg accaaagacg
    10561 acatctacaa aatcctgggc cgcccgcatt acgacgaagg tatgtacggc gtgcgcgaat
    10621 gggattacct gttccacttc cataccccgg gcgtaggtat cgaccctgaa aacacttccg
    10681 gcgtagaaga tgttactacc tgccaataca aagtgatttt cgataaagac aaatttgccc
    10741 gcagcttcta ctggaacccc gtcttcccga aagatgccgc ctgtccgccg cccgcaccca
    10801 aagccgagcc gcaagtcatc atccgcgaaa tcgtgccggc aaaaccgaaa cgtatccgcc
    10861 aataatccga catgccgttc cgcctgtttt tagggatatt atgcggcctg tcaatggttg
    10921 cccccgtata tgcacagggg caaccggatg cggtcaacgc ttatattcaa aagaaaaaag
    10981 tcatcgtaaa cacatccaaa gcggaactct gttttgccga tgacaggcag tgccaccccg
    11041 tcctcatcgg tgttgccacg cccaagggga cgttcgggct gacgctgaac agtaccgaca
    11101 agcctggata cggcggcgaa gtcatcggtt tcaagcagga gggtgatttt cttttcgccc
    11161 tgcaccgcgt ttggaatcag ataccgtcgg aaaggcggaa cgaacgcatc gcctccccgt
    11221 ccgtgtccga caggattatg accaacggct gcatcaacgt cagcgatgcg gtgtacgaaa
    11281 aactgcgtca ttattttgtg ttggaagtga tttgaaacag acggataccg cacgcgccgg
    11341 tatctgtttt cacattgccc cgatgcctga aacaggctgt ccgccacgtc atgccgtctg
    11401 aaaccggcgc agatgccgcc aagccttcag acggcattgc ctgcccgctc cgaccgaaca
    11461 acaaccatct ttgggagaac cttatgcccg aacaaaaccg catcctctgc cgcgaactaa
    11521 gcctgctggc attcaaccgc cgcgtgttgg cacaggcgga agacaaaaac gtcccccttt
    11581 tggaacgcct gcgcttcctg tgcatcgtgt cgtccaatct tgacgagttt ttcgaagtcc
    11641 gcatggcgtg gctcaagcgt gaaaacaagc tgcacccccg gcgcaggctg gacaacggca
    11701 aaatgccgtc tgaaaccatc gccgacgtta ccgaagcggc gcgctccctg atacggcacc
    11761 agtacgacct gttcaacaac gtccttcagc ccgagttggc gcgggaaagc atccattttt
    11821 accgccgccg caactggacg ggcgcgcaga aaaaatggat cgaagactat ttcgaccgcg
    11881 aactgctgcc gatcctgacc cccatcggac tcgacccgtc ccaccccttc ccgcgaccgc
    11941 tgaacaaatc gctcaacttc gccgtcgaac tcgacggcac ggacgcattc ggcaggcctt
    12001 cggggatggc gattgtgcag gcaccgcgca tcctgccgcg cgtcgttccc ctgccgtccg
    12061 aactgtgtgg cggcggacac ggcttcgtct tcctgtcgtc catcctccac gcccacgtcg
    12121 gcaaactctt cccgggcatg aacgtcaaag gctgccacca gttccgcctg acgcgcgaca
    12181 gcgacttgac cgttgacgaa gaagacctgc aaaacctccg cgccgccatc caaaacgaac
    12241 tgcacgaccg cgaatacggc gacggcgtgc ggctcgaagt cgccgacacc tgtcccgcct
    12301 acatccgcga ctttctgctc gcgcaattca aactgaccgc cgccgaactc tatcaggtca
    12361 aaggcccggt caacctcgtg cgcctcaacg ccgtccccga cctagtcaac cgccccgatt
    12421 tgaaatttcc cacacacacg ccgggcagac tgaaagcctt gggcaaaacc gcgtccatat
    12481 tcgatttggt gcgccaatcg cccatcctgc tgcaccaccc ctaccaatcg ttcgaccccg
    12541 ttgtcgaaat gatgcgcgaa gctgccgccg accccgacgt gcttgccgtc aaaatgacga
    12601 tttaccgcac cggcacgcgt tccgaactcg tccgcgccct gatgaaggcg gcactcgccg
    12661 gcaaacaagt aaccgtcgtc gtcgaactga tggcgcgttt tgacgaagcc aacaacgtca
    12721 actgggcgaa gcagctcgaa gaggcgggcg cgcacgtcgt gtacggcgtg ttcggctaca
    12781 aagtccacgc caaaatggca ctggtcatcc gccgcgaaga cggcgtactc aagcgttacg
    12841 cccacctcgg cacgggtaac taccaccaag gcacatcgcg catctacacc gacttcggca
    12901 tcattaccgc cgacgaacaa atcaccgccg atgtgaacac attgtttatg gaaatcacag
    12961 gcttgggcaa acccgggcgg ctgaacaaac tctaccaaag cccgtttacc ctgcacaaaa
    13021 tggttatcga ccgcatcgca cgcgaaaccg aacacgcaaa agccggcaaa ccggcgcgga
    13081 ttaccgccaa gatgaattcg ctcatcgaac cgaccgtcat cgaagccctg tatcgggcaa
    13141 gcgcggcagg cgtacaaatc gatttgattg tgcgcggtat gtgcaccttg cgcccgggtg
    13201 taaaaggctt gtccgaaaac atccgcgtcc gctccatcat cggcaggcag ctcgaacacg
    13261 cgcgcgtgta ttgcttccac aacaacggcg cggacgatac ctttatctcc agcgcggatt
    13321 ggatggggcg caacttcttc cgccgcatcg aaaccgccgc gccgattacc gcgcccgaac
    13381 tcaaaaaacg cgtcatccgc gaaggtttgg aaatggcatt ggcagacaat acgcacgcgt
    13441 ggctgatgca gcccgacggc ggctatatcc gcgccgcgcc cgccgagggc gaatccgaag
    13501 ccgacctgca aaacgatttg tggacactgc tcggaggctg acccgcaccg ccccaatcaa
    13561 aaaccatgcc gtctgaaacc tttccgtttc agacggcata gttttacagc aatctaaaca
    13621 gggcggaccg gagtcaaaaa cacaccttcg ccattcctgc acaagcactt cccctatacg
    13681 ctcccaaccc caagccgccg cattccagac ggcattatag tggattaaat tttaggggct
    13741 gtactagatt agcagatatg ttaccctggg aatatgaaga taacacactg caaattaaag
    13801 aaaaaagtac agaaagaact g
//
LOCUS       DAWKOG010000040        13774 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.040, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000040 DAWKOG010000000
VERSION     DAWKOG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13774
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.040"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(122..1447)
                     /locus_tag="WJK66_000888"
     CDS             complement(122..1447)
                     /locus_tag="WJK66_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEZ7578621.1"
                     /translation="MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPDRPV
                     GIPDPAGTTVGGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGKYMADKGYLKLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFF
                     RELTGSGNDGPVGALGTPLMGEYAGAVDQHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            1660..2499
                     /locus_tag="WJK66_000889"
     CDS             1660..2499
                     /locus_tag="WJK66_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HEZ7578622.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGIGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMILWMKKAARSMKRQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIVVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGEALVWLLYLIPVITWFLCGSRPSETLTRKE
                     ELK"
     gene            2496..3662
                     /locus_tag="WJK66_000890"
     CDS             2496..3662
                     /locus_tag="WJK66_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEZ7578623.1"
                     /translation="MRKFNLTTLSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGS
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLSEADRKALQAPINALAEDLAQLRGILGLK"
     gene            3806..5071
                     /gene="efeB"
                     /locus_tag="WJK66_000891"
     CDS             3806..5071
                     /gene="efeB"
                     /locus_tag="WJK66_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HEZ7578624.1"
                     /translation="MSTNNPTQPTRRTLFKTALAAGVGGAIGGYLGGKGLGETAERTA
                     ESQHSPQAYPCYGKNQAGIVTPQQAFSIMCAFDVTAQNAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPIHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTAQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGNTTPKDSHMRLANPR
                     DPEFLKKHRLFRRAYSYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(5141..5470)
                     /locus_tag="WJK66_000892"
     CDS             complement(5141..5470)
                     /locus_tag="WJK66_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEZ7578625.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAEEVR
                     DANGAALQNGDTVILIKDLKVKGSSMTIKQGTKVKGIRLQEGDHNISCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(5545..6915)
                     /gene="glmU"
                     /locus_tag="WJK66_000893"
     CDS             complement(5545..6915)
                     /gene="glmU"
                     /locus_tag="WJK66_000893"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEZ7578626.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHEIGGETMLGRVIDTAA
                     ALNPQNICVVVGHGKEQVLDTVKRDVVWVEQTEQLGTGHAVKTALPHLAAEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPADPSGLGRIIRDGSGSVTAIVEEKDASAA
                     QKAVKETNTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVQVRA
                     SYLAAGVNNKCQLAELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVIIDAN
                     CIFEGEIELGDNVEIGANCVIKNAKIGANSKIAPFSHLESCEVGENNRIGPYARLRPQ
                     ARLADDVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVNKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSTITRNVEDNKLALARARQTVIEGWVR
                     PEKDKQ"
     gene            complement(6970..7242)
                     /locus_tag="WJK66_000894"
     CDS             complement(6970..7242)
                     /locus_tag="WJK66_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HEZ7578627.1"
                     /translation="MRKTFLILMTTAALLSGCAWETYQDGNGKTAVRQKYPAGTPVYY
                     QDGSYSKNMNYNQYRPERHAVLPNQTGNNADEEHRQHWQKPKFQNR"
     gene            complement(7268..7948)
                     /locus_tag="WJK66_000895"
     CDS             complement(7268..7948)
                     /locus_tag="WJK66_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEZ7578628.1"
                     /translation="MQENPTVWLFDLDNTLHDADAGIFTLINRAMTRYMARRLKLSES
                     AASDLRQEYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDAILTKLHGMADTEHTLCR
                     LNGRKAVFSNGPSFYVRAVVGALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVWFGAKSHALSFIDACARDMAQLARYAETLSEH
                     RQTHYNTL"
     gene            8007..9008
                     /locus_tag="WJK66_000896"
     CDS             8007..9008
                     /locus_tag="WJK66_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7578629.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGLGEEGAFKWWVQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPKLAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            9102..9950
                     /locus_tag="WJK66_000897"
     CDS             9102..9950
                     /locus_tag="WJK66_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ7578630.1"
                     /translation="MDFKQFDFLHLISASGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            9976..10461
                     /locus_tag="WJK66_000898"
     CDS             9976..10461
                     /locus_tag="WJK66_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEZ7578631.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGMLAAAFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(10563..12131)
                     /gene="msrAB"
                     /locus_tag="WJK66_000899"
     CDS             complement(10563..12131)
                     /gene="msrAB"
                     /locus_tag="WJK66_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HEZ7578632.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAATLKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSYNMRRTEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            12277..13542
                     /gene="ftsY"
                     /locus_tag="WJK66_000900"
     CDS             12277..13542
                     /gene="ftsY"
                     /locus_tag="WJK66_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEZ7578633.1"
                     /translation="MFSFFRRKKKQETPAPEEAQVQETAAKAESEVTQIVENIKEEVE
                     SLTESVKGQVESAVETVSGAVEQVKEAVAEMLSEAEEAAEKAAEQVEAAKEAIAETVG
                     EAVGQVQEAVATTEEHKLGWVARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGESIDDLRPFDARAFVDALLD"
BASE COUNT         2957 a         3527 c         4123 g         3167 t
ORIGIN      
        1 aatttgatcc actataattc cgtcaaataa gaaaggaatt ttgtgcctgc ggtatcgcaa
       61 aacttagcct taatgcgccc gattgcctag ggatgggctt cagatggcat tgttttccgg
      121 tttacgggcg gtattcgggc ttcataccgt tgggtaggag ctgccagacg taacccgtgg
      181 ttttctgttt gccggcaagt tcgccggctt cgtcgccgta tccccaaaaa taatccacgc
      241 gcaccgcgcc tttaatcgcg ctgccggtat cctgcgccat aatcaggcgg ttgagggctt
      301 tgcgggtaac cggatgggcg gtggcgacaa ataagggcgc acccaaggta atgtagtgct
      361 ggtcgactgc gccggcatat tcccccatca gcggcgtgcc cagtgcgccg acagggccgt
      421 cattgccgct tccggtaagc tcgcggaaaa agatatagct ggggttttga cccaaaactt
      481 cggcgaggcg ttgcggattt tgccgcatat aagacttaat gccctgcatg gaggtttgtc
      541 cgagtttgag gtagccctta tccgccatgt attttccgat ggaaacatag ggatgctcgt
      601 ttttgtcggc atagccgatg cggatgtatt tgccggacgg ggttttcaga cggcccgagc
      661 cttggatgtg catgaaaaag agttcgacgg ggtcttcggc gtaaccgagt atcggtgctt
      721 tgccgtcaag cgcgccgccg ttgatttggt ttcgcgtgtg gtaagggagg aagcggcttc
      781 cttcaaacct gcccttgatt gccgttgtgc gtgcggtgat ggggaatcgg gagaggtcgg
      841 cggtatgtgt gccgccggta ttgtcgattg tgccgctgtt ttttcccgtc tgcctgatgc
      901 ggacaagggc ttttccgctc cgcaaaccgg caggcagggg gacggagata aaatcgtcgg
      961 gaataccgta aatcgggaag cgggcttgtg ccgtccgcct gtcgtcgccc ttcagcaccg
     1021 gctcgtaata gccggtaacc gtaccggcaa ggcttccgtt gcctgcaacc tgccacggcg
     1081 tgaaatagcg ttcaaaaaac tgttttgcct gaaaggaatg gacgggggtt tgaaaggctt
     1141 gggcgcacac atcctgccag ccttggcggt ttttcaaatt ggcgcagccg aggcggaagg
     1201 attgcaggct tttggcgaaa tcctgcgccg cccagtgggg cagggacagg tgcggcacaa
     1261 cggtataaac ggccccgccg ccgccgaccg tcgttccggc ggggtcgggg atgccgaccg
     1321 gccggtccgg gccgttgatg acggatgtgt cgggttgcgg aaaggtttgg atgctcttgc
     1381 tttggcaggc ggcgaggatg gcggcggcga tgccgcacag ggcggcgcgg aacaggtatt
     1441 ttttcataat ggacaatgtt gccggcagta ataaaaaaaa gatggtttcg ggcggcgttg
     1501 cggcagccgt ggagagggga ttttaacaca gggcgcagct gcagcctgcg gaactttccg
     1561 ccgcgcggta ctgcagataa aaataacttg catttgtatt tacaagcaat gaaaatattc
     1621 cgataatata ttattcatca tccttgttcg ttcgcgttta tgctggtcgc ttttttaatt
     1681 atgttgcgcg agggtatcga ggccgcgctc atcgtcggca tcgttgccgg ttttctgaaa
     1741 cagtccggac attccaaact gatgcctaag gtctggttcg gggtcgtcct tgcttctttg
     1801 atgtgtttgg gcatcggata cggcatccat tcggcaacgg gcgagattcc ccagaagcag
     1861 caggagttcg tcgtcggcat tatcggtttg gttgctgtcg cgatgctgac ttatatgatt
     1921 ttatggatga aaaaggcggc gcgttcgatg aagcggcagc ttcaggattc tgtacaggcg
     1981 gctttgaacc gtggcagcgg tcaaggatgg gccttggtcg gtatggcgtt tcttgccgtg
     2041 gcgcgcgaag gtttggagag cgtttttttc ctgcttgccg tattcaaaca gagcccgacg
     2101 tggcagatgc cggccggtgc ggtagcgggg gttttggctg ccgccgtgat tggcgcgttg
     2161 atttatcagg gtgggatgcg cctgaatctg gcgaagtttt tccgttggac gggggcgttt
     2221 ttgattgtcg ttgccgccgg cctgcttgcc ggctcgctgc gcgcgctgca cgaagcaggc
     2281 atttggaacg cgcttcagga cattgtgttc gactcatcaa aatatttgca cgaagacagt
     2341 ccgttaggtg tgctgctcgg cggatttttc ggctataccg accatccgac gcagggcgag
     2401 gccttggttt ggctgctgta ccttattccc gtcataactt ggtttttgtg cggcagcagg
     2461 ccgtctgaaa ctttaacccg taaagaggag ctgaaatgag aaaattcaat ttgaccacat
     2521 tgtccgtgat gcttgcctta ggtttgaccg cgtgccagcc gccggaggcg gagaaagctg
     2581 cgccggccgc gtccggtgag gcgcaaaccg ccaacgaggg cggttcggtc agtatcgccg
     2641 tcaacgacaa tgcctgcgaa ccgatggaac tgaccgtgcc gagcggacag gttgtgttca
     2701 atattaaaaa caacagcggc cgcaagctcg aatgggaaat cctgaaaggc gtgatggtgg
     2761 tggacgagcg cgaaaacatc gcccccggac tttccgataa aatgaccgtc accctgttgc
     2821 cgggcgaata cgaaatgact tgcggtcttt tgaccaatcc gcgcggcaag ctggtggtaa
     2881 ccgacagcgg ctttaaagac accgccaacg aagcggattt ggaaaaactg tcccaaccgc
     2941 tcgccgacta taaagcctac gttcaaggcg aggttaaaga gctggtggcg aaaaccaaaa
     3001 cctttaccga agccgtcaaa gcaggcgaca ttgaaaaggc gaaatccctg tttgccgaca
     3061 cccgcgtcca ttacgaacgc atcgaaccga ttgccgagct tttcagcgaa ctcgaccccg
     3121 tcatcgatgc gcgtgaagac gacttcaaag acggcgcgaa agatgccgga tttaccggct
     3181 ttcaccgtat cgaatacgcc ctttgggtgg aaaaagacgt gtccggcgtg aaggaaattg
     3241 cagcgaaact gatgaccgat gtcgaagccc tgcaaaaaga aatcgacgca ttggcgtttc
     3301 ctccgggcaa ggtggtcggc ggcgcgtccg aactgattga agaagtggcg ggcagtaaaa
     3361 tcagcggcga agaagaccgg tacagccaca ccgatttgag cgacttccaa gccaatgtgg
     3421 acggttcgaa aaaaatcgtc gatttgttcc gtccgctgat tgaggccaaa aacaaagcct
     3481 tgttggaaaa aaccgatacc aacttcaaac aggtcaacga aattctggcg aaataccgca
     3541 ccaaagacgg ttttgaaacc tacgacaagc tgagcgaagc cgaccgcaaa gcgttacagg
     3601 cccctattaa cgcgcttgcc gaagaccttg cccaacttcg cggcatactc ggcttgaaat
     3661 aagccttaag cgttcagacg gcatttagcg acagatgccg tctgaaattt tatctgcaaa
     3721 accggaaagg tattgaaaaa ggcggaggtt tccgtttccc gcgttccgcc gtatcggata
     3781 ccgagtttaa ataaaggata aaaacatgag tacgaacaac ccgacacaac cgaccaggcg
     3841 caccctcttt aaaaccgcac tcgcagccgg cgtgggcggc gcaatcggag gttatctcgg
     3901 tgggaagggg ctgggtgaaa ctgccgaacg caccgccgaa agccaacact cgccccaagc
     3961 ctatccctgt tacggaaaga atcaggcagg tatcgttacg ccgcagcagg cattttccat
     4021 catgtgcgcc tttgacgtta ccgcgcaaaa cgccaagcag ctggaaaacc tgttccgcac
     4081 gctgactgcc cgtatcgagt ttctcactca aggcggtgaa taccaagacg gcgacgacaa
     4141 acttccgccg gccggcagcg gcattttggg caaagccttc aaccccgacg gattgaccgt
     4201 taccgtgggg gtgggcagca gcctgtttga cggacgattc ggactcaaag acaaaaaacc
     4261 gattcatttg caggaaatgc gcgacttctc caacgacaag ctgcaaaaaa gctggtgcga
     4321 cggcgatttg agcctgcaaa tctgcgcctt tacccccgaa acctgccaag ccgccctgcg
     4381 cgacatcatc aaacacaccg cccaaaccgc cgttatccgc tggagtatcg acgggtggca
     4441 gcccaaatcc gaacccggcg cgatggcggc gcgcaacctg ttgggcttcc gcgacggcac
     4501 gggcaacccc aaagtttccg accccaaaac cgccgacgag gttttgtgga cgggtgtggc
     4561 cgccaacagc ctagacgaac cggagtgggc gaaaaacggc agctatcagg cagtccgcct
     4621 tatccgccac tttgtcgagt tttgggacag gacgccgctt caagagcaaa ccgacatttt
     4681 cgggcggcgc aaatacagcg gcgcgccgat ggacggcaaa aaagaagccg accaaccgga
     4741 ttttgccaaa gaccccgagg gcaataccac gcccaaagac agccatatgc gcttggcgaa
     4801 tccgcgcgat cccgaattcc tcaaaaaaca ccgcctcttc cgccgcgcct acagctattc
     4861 gcgcggactc gcctcaagcg gacagcttga tgtcgggctg gtgttcgtct gctatcaggc
     4921 aaaccttgcc gacggattca tcttcgtgca aaacctcctc aacggcgaac cgctggaaga
     4981 atacatcagc cccttcggcg gcggctattt cttcgtgctg cccggcgtgg aaaaaggcgg
     5041 cttcttggga caagggctgc tgggcgtata aacctgcctg acggacaaat gccgtctgaa
     5101 tccgataagg ggttcagacg gcattttagt gtttgggcgg tcaggctttt ttgacgaatt
     5161 cagattttaa attcatcgcg ctgccgtcga ttttgcagga gatgttgtga tcgccttcct
     5221 gtaggcgtat gcctttgact tttgtacctt gtttgatcgt catcgagctg ccttttacct
     5281 tgaggtcttt gatgaggatg acggtgtcgc cgttttgcag cgccgcgccg ttggcatcgc
     5341 gcacttcttc cgcaaggtcg gcggcggatt cggtttcatt ccattcgtgg gcgcattcgg
     5401 ggcagatgta ttgtccgccg tcttcatagg tgtattcgga ggcgcattgc gggcatgggg
     5461 gtaatgacat ggttttccgt ccttatcgga tgtttgtttt ggggtgccgt ctgaaacctg
     5521 aaaccggctt cagacggcat agctttattg tttgtctttt tcaggacgca cccagccttc
     5581 gatgacggtt tggcgggcgc gggcgagggc gagtttgttg tcttcgacat tgcgggtaat
     5641 cgtgctgcct gcgcctgtgg ttactttgtt gcccagtgtt acaggggcaa ccagtacgca
     5701 gtttgaaccg atgcggactt cgtcgccgat gacggttttg tgtttgttca cgccgtcgta
     5761 gttggcaata atcgtacctg cgccgaagtt ggttttgctg ccgacttcgg catcaccaat
     5821 gtaggtgagg tggttggctt tggtgccttt gccgatggcg gcgtttttga tttcgacgaa
     5881 gttgccgacg tgtacgtcgt ctgcaaggcg ggcttgcgga cgcaggcggg cgtatgggcc
     5941 gattcggttg ttttcgccga cttcgcagct ttcgaggtgg gagaaggggg cgattttgct
     6001 gtttgcaccg attttggcgt ttttgatgac gcagtttgcg ccgatttcga cgttgtcgcc
     6061 gagctcgatt tcgccttcaa agatacagtt cgcatcaatt atgacatctt gtccgtgttt
     6121 cagacggccg cgtaaatcga aacgtgccgg atcgcgcagg gttacgcctg ctttgagcaa
     6181 ttcttgtgcc tgttcggttt ggaagatgcg ttcgagttcg gcgagctggc atttgttgtt
     6241 cacgccggcg gcgaggtagg aggcgcgcac ttggacggga tggactttga tgccgtcggc
     6301 aacggctttg gcgatgaggt cggttaaata atattcgcct tgggcattgt tgctggaaag
     6361 gctgttcagc cagttttcca gtttggcgtt gggcaggacg agaatgcctg tgttggtttc
     6421 tttcacggct ttttgggcgg cacttgcgtc tttttcttcg acaatggcgg ttacgctgcc
     6481 gctgccgtcg cggatgatgc gccccaaacc tgacgggtcg gcgggcacgt cggtcaacag
     6541 cccgacttcg ttgcctgcgg cttcgagcag ggtttcgagg gtttcaacgt caattaaagg
     6601 aacgtcgccg tacaacacca gcgtgcggcc ttcggcggca aggtggggca gggcggtttt
     6661 gacggcgtgg ccggtgccga gctgttcggt ttgttcaacc cagacgacat cgcgtttgac
     6721 ggtgtccaaa acttgctctt tgccgtggcc gacgacgacg cagatgtttt gcggattcag
     6781 cgcggcggcg gtgtcgataa cgcgcccgag cattgtttcg ccgccgattt cgtgcaaaac
     6841 cttgggcatt ttggaataca tgcgcgtgcc tttgccggcg gcgaggatga cgatgtttaa
     6901 agtgttttgc ggcatagcgt tttcctgtgc aatgccgtct gaagcggctt cagacggcat
     6961 cgggcgggtt tatcggtttt gaaatttggg cttttgccag tgttggcgat gctcttcgtc
     7021 ggcgttgttg ccggtttggt tgggcaacac ggcatggcgt tcggggcggt attggttgta
     7081 gttcatattt ttcgagtagc tgccgtcttg gtaataaacg ggcgtgcctg cgggatattt
     7141 ttgacggacg gcggtcttgc cgttgccgtc ttgataagtt tcccacgcgc agcccgacaa
     7201 aagggcggcg gtggtcatca ggataaggaa ggttttacgc atggcttttc tttcgtattt
     7261 tcaggattta gagggtattg taatgagttt ggcggtgttc tgacaaagtt tctgcatacc
     7321 gagccagttg cgccatatcg cgtgcgcagg cgtcgataaa ggacagcgcg tgggattttg
     7381 caccgaacca gacggttttc atgcccaatg cttttgcctg atgcaggttg tccgcgctgt
     7441 cgtccaccat aatgcagcat tcgggcggta cgtccaaaag gcggcagaca ttgagatacg
     7501 cttgcggatt gggtttgtac agcagtccga aatcatccgt gccgaacagt gcgtcgaaac
     7561 ggttttccaa accgagtgcg ccgacaacgg cacggacgta aaacgacggg ccgttggaaa
     7621 aaaccgcctt gcgcccgttc agacggcata gggtgtgttc cgtatccgcc ataccgtgca
     7681 gcttggtcag gattgcatcg atcggatggc tttcgcgtaa aaattcggcg atgtcgattt
     7741 cgggatggtg gatttgcagt ccggcgagcg ttgcgccgta gcggtgccaa tattcctgac
     7801 gcaggtcgga cgcggcggat tcggagagtt tgaggcggcg tgccatatag cgtgtcatag
     7861 cgcggttgat gagtgtgaag atgcctgcgt cggcatcgtg cagcgtgttg tcgaggtcga
     7921 acagccacac ggtcgggttt tcttgcattt tgaaccgtga aaatttgtta gaatgttatt
     7981 ttacagcgaa tagaggagga ctcggaatga aacggaaaat ttggctgctg ccgctgctgg
     8041 cggtttcggc atacctgcag gcgcagacgg aagtcaggct ggcggtgcat aagtcgttca
     8101 gcctgcccaa agggctgatt gcgcgcttcg agcgggcaaa cgatgcgaag gtgtcgatta
     8161 ttcaggcggg cggcgcgaac gaaatgctca acaaactgat tttgagccgc gccaacccga
     8221 tcgccgacgc ggtgtacggc ttggacaacg ccaatatcgg caaggcgcgg gaaatgggca
     8281 ttttggcggc ggcgcaaccc gaatccgccc ccgtcgcggt cgggctgcct tcggctttgg
     8341 cggtcgatta cggctatgtg tccatcaatt acgacaaaaa atggtttgaa ggcaaaaagc
     8401 ttcctctgcc gcaaaccctg caggatttga cccgccccga atataaaaac ctattggtcg
     8461 tgccgtctcc cgccacgtcg tccccggggc tgggcttcct gatggcgaac atcagcggtc
     8521 tgggcgaaga aggcgcgttc aaatggtggg tacagatgcg gcagaacggc gtgaaggtcg
     8581 ccaaaggctg gagcgaggcg tattacaccg acttttcgca caacggcggc gcgtatccgc
     8641 tggtggtcgg ttatgccgcc agcccggcgg cggaagtgta tttttccaaa ggcaaataca
     8701 gcgagccgcc gacgggcaac ctgtttttaa aaggcggcgt attccgccag gtcgaaggcg
     8761 cggcagtctt gaagggcgcg aaacagccga agctggcggc aaaactggtg caatggctgc
     8821 aaagtcggga agtgcagcag gcggttccgt ccgaaatgtg ggtttacccc gccgtcaaaa
     8881 acacgcgcct gcccgacgtg ttccgcttcg cccaagcccc gacgcacacc accgcccccg
     8941 cgcagcgcga tattgatgcg aaccagcgcg gatgggtttc ccgttggatt agaacggttt
     9001 tgaaataaaa caaacatacc gccccgcagg gcttcatacg gcatttttac acctgtgccg
     9061 attacgccgc acggggcgga tgttcgatca agaggaaaac aatggacttc aaacaatttg
     9121 attttttaca cctgataagt gcttccggct gggagcatct ggctgaaaag gcgtgggcgt
     9181 tcgggctgaa ccttgccgcc gcgctgctta tttttttggt cggaaaatgg gcggcgaaac
     9241 gcattgtcgc cgtgatgagg gcggcgatga cgcgcgcgca ggtcgatgcc acgctgatta
     9301 gttttttgtg taatgttgcc aatatcggct tattgatttt ggtgattatt gccgcattgg
     9361 gcagattggg cgtttccaca acatccgtaa ccgccttaat cggcggcgcg ggtttggcgg
     9421 tggcgttgtc cttgaaagac cagctgtcca attttgccgc cggtgcgctg attatcctgt
     9481 ttcgcccgtt caaagtcggc gattttatcc gcgtcggcgg ttttgaagga tatgtccgag
     9541 agattaaaat ggtgcagact tctttgcgga cgaccgacaa cgaagaagtc gtgctgccca
     9601 acagcgtggt gatgggcaac agcatcgtca accgttccac actgccgctg tgccgcgccc
     9661 aagtgatagt cggcgtcgat tacaactgcg atttgaaagt ggcgaaagag gcggtgttga
     9721 aagccgccgt cgaacacccc ttgagcgttc aaaacgaaga gcggcaggcc gccgcctaca
     9781 tcaccgcctt gggcgacaat gccatcgaaa tcacattatg ggcttgggca aacgaagcag
     9841 accgctggac gctgcaatgc gacttgaacg aacaagtggt cgaaaacctc cgcaaagtca
     9901 atatcaacat cccgttcccg caacgcgaca tacacatcat caattcttaa acgccgtctg
     9961 aaaggggagt gggaaatgga cgcgctgcac accatcgccc gaaacctgac gaaaaaacgt
    10021 caaaccgtaa gctgtgccga atcctgtacg ggcggaatgc ttgccgccgc attcacaagc
    10081 gttgcaggca gttcgcaatg gttcgaccag agttttgtaa catacagcaa caaagccaaa
    10141 gaagaccgct tgggcgtgtt gcccgaaacc ctgctcgaac acggcgcggt cagccgccaa
    10201 accgtctatg agatggcgcg cggcgcgaaa gccgtggcgc aggcggatta cgccgtcggt
    10261 atttccggca tcgccggacc gggcggcggc agcgaaagca aacccgtcgg cacagtttgg
    10321 ttcgggtttg cctttccggg cggaagttgc gaagcaatgc gccgttttga tggcaaccgc
    10381 gaatccgtcc gcgcgcaggc ggtcgccttc gcgttggaac ggttggcggg gctgattgaa
    10441 aacggcggcg atgccgtcta aacaaaatct ccgtctggac aaaatccccg ccggataaaa
    10501 aataccgtct gaaacgttgc aggtttcaga cggtattttg tcgggttagg cggcggtgcg
    10561 gcttatttca ctttactctt caacgcgccg tagcctgccg cgtccatttg ttccagcggg
    10621 atgaatttca agctcgcgcc gttgatgcag tagcgcagtc cgcctttgtc gcgcgggccg
    10681 tcggggaaga cgtgtcccaa atgcgagtcg gcggcgtggc tgcgcacttc ggtgcggcgc
    10741 atgttgtagc tgaaatcatc gtgttcggta acggattttg catcaatcgg gcgcgtgaag
    10801 ctcggccagc cgcagccgga atcatatttg tcggcggagc tgaacaaagg ttcgccgctg
    10861 acaacgtcca cataaatgcc gggtttgaac aaatggtcgt attcgtggct gaaggcatat
    10921 tcggtcgcgc tgttttgggt cacttggtac tgctcttcgg tcagggtgcg tttgagttcg
    10981 gcgtcactcg gttttttata cgttgccgcg tcgaagcctt tgccttgcgg tgcggtcttg
    11041 gttttgcccg gcagcggttc gtcggctttg cggatatcga tgtggcagta gccgttgggg
    11101 tttttaatca agtagtcctg atggtattcc tcggcatcgt agaagttttt cagcggctcg
    11161 ttttcaacaa cgaggggcag ttggtatttt tgctgctcgc gtttgagggt ggcggcgatg
    11221 acggcttttt cggcggggtc ggtgtagtac acgccgctgc ggtattgcgt accggtgtcg
    11281 ttgccctgtt tgttgaggct ggtcggatca acgacgcgga agaaatattg caggatgtcg
    11341 tctaggctga gtttgtcggc atcgtaggtc actttgacgg tttcggcgtg gcccgtatgg
    11401 cggtaggaca cgtcttcata gctcggattt ttcgtgttgc cgttggcgta gccggatacc
    11461 gcgtcaacca cgccgtcgat gcgttggaaa taggcttcca agccccagaa gcagccgccg
    11521 gcgaggtaaa tggtgcgcgt gttcatgatt tttgaatcct ttttctgagt gtcgggtttg
    11581 tagaacgaat gtttcaaact gcccaaatcg gcattcgggt cgcggattaa cgccaacgcc
    11641 tgcgcttcgt tgatgctgcc tttgacgatg cgctgcacgt cgccgtcttt accgattaac
    11701 gcccacgaag ggtaaacgct gatattcagg ctttgggcga tcgtgccgcc gttgtcggtt
    11761 acgacgggca gcttgggata attcaaaccg gcataccatt tttggaagtc gccgtctttt
    11821 ttctcgtgca aaaagcccgg agacgcgacg gtaatcaggt tggcggagct gaattttgca
    11881 tcttgcgccc atttttcggt ctgtcccaat tcggacaaac acaaaggaca ccagctcgcc
    11941 caaaatttaa tcagcgtcgg tttgtctttt ttcaagtaaa cactggcggg gcggttgtct
    12001 gcggttttca aagtggataa agtgtgcggc acggtcgcgg ctccggcatc gacgattttg
    12061 ggcgaacaag cacccagcgc aagcaggcag ccgaacttgg cgcaaaggga aaagaaagta
    12121 cggtgtttca ttttaatgtt tcctgtgtgg acggtttgca tgattagacg tttgagatgc
    12181 cgaaacctta cagctcggat tttcagacag ccttgccgcg taaaatacgc tacaatacgc
    12241 cctatttcaa gtttctaaaa ttaaaaggaa aattcaatgt tcagcttctt ccgtcgcaag
    12301 aaaaaacagg aaacgccggc tcccgaggag gctcaagttc aggaaactgc agcaaaagca
    12361 gaatccgaag ttactcaaat agttgaaaat attaaagaag aagtcgaatc tttaacggaa
    12421 agcgtcaaag ggcaggtcga atcggctgtt gaaaccgtca gcggtgcggt tgaacaagta
    12481 aaggaagccg ttgccgagat gctgtctgaa gcagaggaag cggcggaaaa agcagcggaa
    12541 caagtcgaag cggcaaaaga agccattgcc gaaaccgtcg gcgaggctgt cgggcaagtt
    12601 caagaagccg ttgcaacaac tgaagaacac aagctcggtt gggtggcgcg tttgaaacaa
    12661 ggtttgacca aatcgcgcga caaaatggcg aaatcgctgg cgggcgtgtt cggcggcgga
    12721 caaatcgacg aagatttgta cgaagagctg gaaaccgtgc tgattaccag cgacatgggc
    12781 atggaagcca ccgaatacct gatgaaagac gtgcgcgacc gcgtcagcct caaagggctg
    12841 aaagacggca acgaattgcg cggcgcgttg aaagaagcct tgtacgacct gattaagcct
    12901 ctggaaaaac cattggtttt gcccgaaacc aaagagccgt ttgtcatcat gcttgccggc
    12961 atcaacggcg cgggcaaaac cacgtctatc ggcaaactcg ccaaatattt ccaagcgcag
    13021 ggcaaatctg tgctgcttgc cgccggcgat actttccgcg ccgccgcccg tgagcagctt
    13081 caagcttggg gcgaacgcaa caacgtaacc gtgatttcgc aaaccacggg cgattccgcc
    13141 gccgtgtgct tcgatgccgt ccaagccgcc aaagcgcgcg gcatcgacat tgtgctggcc
    13201 gacaccgccg gccgcctgcc cacgcagctt catttgatgg aagaaatcaa aaaagtgaaa
    13261 cgtgtgctgc aaaaagccat gcccgacgcg ccgcacgaaa tcatcgtcgt gcttgatgcc
    13321 aacatcgggc aaaacgccgt caaccaagtc aaagcctttg acgacgcatt agggctgacc
    13381 ggtttaatcg tgaccaaact cgacggcacg gcaaaaggcg gcatcctcgc cgcgcttgct
    13441 tccgaccgcc ccgtccccgt ccgctacatc ggcgtgggcg aaagcataga cgacctgcgc
    13501 ccgtttgacg cgcgcgcgtt tgtggacgca ctgctggatt gagccgaaat gccgtccgaa
    13561 aacagcagac cgatgccgtc attcccgcgc aggcgggaat ctagaccttg ggataacagc
    13621 aatattcaaa ggttataaaa gacccgtcat tcccgcgcag gcgggaatct agaaccatcg
    13681 gcacggaaac ttatcgggta aacggtttct tgagattccg cgtcctagat tcccactttc
    13741 gtgggaatga cgggatttta ggtttctgat tttg
//
LOCUS       DAWKOG010000041        13616 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.041, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000041 DAWKOG010000000
VERSION     DAWKOG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13616
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.041"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..512)
                     /locus_tag="WJK66_000901"
     CDS             complement(<1..512)
                     /locus_tag="WJK66_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEZ7578634.1"
                     /translation="MAFTLMRRAMIRKMPYTEDMRPGDTANPYGASKAMVERMLTDIQ
                     KADPRWSVILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVASGRLPQLSVFGGDY
                     PTPDGTGMRDYIHVMDLAEGHIAAMKAKGGLSGVHLFNLGSGRAYSVLEIIRAFEAAS
                     GLNIPYQIQPR"
     gene            complement(555..899)
                     /locus_tag="WJK66_000902"
                     /pseudo
     CDS             complement(555..899)
                     /locus_tag="WJK66_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228843.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
     gene            complement(892..1894)
                     /gene="dcm"
                     /locus_tag="WJK66_000903"
                     /pseudo
     CDS             complement(892..1894)
                     /gene="dcm"
                     /locus_tag="WJK66_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(1925..4198)
                     /locus_tag="WJK66_000904"
     CDS             complement(1925..4198)
                     /locus_tag="WJK66_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEZ7578635.1"
                     /translation="MNITQILSQELSATAAQITAAVELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQSQDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETEGEKFSDYFDHREPIRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     EMSKMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSRLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGISAAELIGNRERVKQIKVSDFTDERFGLPTILDILSELEKPGRDPRGEFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            complement(4262..4912)
                     /locus_tag="WJK66_000905"
     CDS             complement(4262..4912)
                     /locus_tag="WJK66_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ7578636.1"
                     /translation="MISVEHVSKRYLTRQGWRTVLHDISFKMEKGEKIGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMYQFEDMDKAYEYYNSLP"
     gene            complement(4909..5706)
                     /locus_tag="WJK66_000906"
     CDS             complement(4909..5706)
                     /locus_tag="WJK66_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ7578637.1"
                     /translation="MKELHKTSFLESLLIQKRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLLTLVMVLMWKFFRMHNVSALNIVAFTLTGYPMMMMWRNASNHAIGSISANTSLL
                     YHRNVRVLDTIFARMLLEIAGATIAQVVIMFALVIIGWIDVPADIFYMLLAWLLMAMF
                     AVGLGLVICSVAFHFEPFGKVWSTISFVMMPLSGVFFFVHNLPQQLQHYVLMIPMVHG
                     TEMFRAGYFGDSVTTYENPWYILLCNLVLLLLGLAVVARFSKGVEPQ"
     gene            complement(5706..6869)
                     /locus_tag="WJK66_000907"
     CDS             complement(5706..6869)
                     /locus_tag="WJK66_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export inner-membrane
                     protein CtrB"
                     /protein_id="HEZ7578638.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVQEYMQSR
                     SALDALRKKMPIRDFYEKEGDIFSRFNGFGLRGEDEAFYQYYRDKVSIHFDSVSGISN
                     LSVTSFNAGESQKINDALLKQGEVLINQLNERARQDTIRYAQEVVNSAEEQVKEASVQ
                     LTKFRVSNGIFDLKAQSDVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLIARE
                     KSLRKEISQQMKAISGGGEGSLSNQAAEYQRVYLENELAEKQLAAAMTSLESAKAEAD
                     RQQLYLEVISQPNKPDLAYEPNRLYNIVATFVIGLIVYGIVVLLSASIREHKN"
     gene            complement(6884..8047)
                     /gene="ctrA"
                     /locus_tag="WJK66_000908"
     CDS             complement(6884..8047)
                     /gene="ctrA"
                     /locus_tag="WJK66_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HEZ7578639.1"
                     /translation="MLKLKFCIVISFLILGSACSAIPSSGPSAKKVVSLGQQSEAQIP
                     EVELIDVNHAVAQSLYKAQVNQSFTQFGDGYASTGTLNIGDVLDIMIWEAPPAVLFGG
                     GLSSMGSGSAQQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKKMANQ
                     PQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNVQLTR
                     GNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSARGLSL
                     SEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVYRVNM
                     ADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            8259..9377
                     /gene="wecB"
                     /locus_tag="WJK66_000909"
     CDS             8259..9377
                     /gene="wecB"
                     /locus_tag="WJK66_000909"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEZ7578640.1"
                     /translation="MKVLTVFGTRPEAIKMAPVILELQKHNTITSKVCITAQHREMLD
                     QVLSLFEIKADYDLNIMKPNQSLQEITTNIISSLTDVLEDFKPDCVLVHGDTTTTFAA
                     SLAAFYQKIPVGHIEAGLRTYNLYSPWPEEANRRLTSVLSQWHFAPTEDSKNNLLSES
                     IPSDKVIVTGNTVIDALMVSLEKLKITTIKKQMEQAFPFIQDNSKVILITAHRRENHG
                     EGIKNIGLSILELAKKYPTFSFVIPLHLNPNVRKPIQDLLSSVHNVHLIEPQEYLPFV
                     YLMSKSHIILSDSGGIQEEAPSLGKPVLVLRDTTERPEAVAAGTVKLVGSETQNIIES
                     FTQLIEYPEYYEKMANIENPYGIGNASKIIVETLLKNR"
     gene            9379..11016
                     /locus_tag="WJK66_000910"
     CDS             9379..11016
                     /locus_tag="WJK66_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide phosphotransferase SacB"
                     /protein_id="HEZ7578641.1"
                     /translation="MFILNNRKWRKLKRDPSAFFRDSKFNFLRYFSAKKFAKNFKNSS
                     HIHKTNISKAQSNISSTLKQNRKQDMLIPINFFNFEYIVKKLNNQNAIGVYILPSNLT
                     LKPALCILESHKEDFLNKFLLTISSENLKLQYKFNGQIKNPKSVNEIWTDLFSIAHVD
                     MKLSTDRTLSSSISQFWFRLEFCKEDKDFILFPTANRYSRKLWKHSIKNNQLFKEGIR
                     NYSEISSLPYEEDHNFDIDLVFTWVNSEDKNWQELYKKYKPDFNSDATSTSRFLSRDE
                     LKFALRSWEMNGSFIRKIFIVSNCAPPAWLDLNNPKIQWVYHEEIMPQSALPTFSSHA
                     IETSLHHIPGISNYFIYSNDDFLLTKPLNKDNFFYSNGIAKLRLEAWGNVNGECTEGE
                     PDYLNGARNANTLLEKEFKKFTTKLHTHSPQSMRTDILFEMEKKYPEEFNRTLHNKFR
                     SLDDIAVTGYLYHHYALLSGRALQSSDKTELVQQNHDFKKKLNNVVTLTKERNFDKLP
                     LSVCINDGADSHLNEEWNVQVIKFLETLFPLPSSFEK"
     gene            11089..11832
                     /locus_tag="WJK66_000911"
     CDS             11089..11832
                     /locus_tag="WJK66_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEZ7578642.1"
                     /translation="MLSNLKTGNNILGLPEFELNGCRFLYKKGIEKTIITFSAFPPKD
                     IAQKYNYIKDFLSSNYTFLAFLDTKYPEDDARGTYYITNELDNGYLQTIHCIIQLLSN
                     TNQEDTYLLGSSKGGVGALLLGLTYNYPNIIINAPQAKLADYIKTRSKTILSYMLGTS
                     KRFQDINYDYINDFLLSKIKTCDSSLKWNIHITCGKDDSYHLNELEILKNEFNIKAIT
                     IKTKLISGGHDNEAIAHYREYFKTIIQNI"
     gene            11834..12697
                     /locus_tag="WJK66_000912"
     CDS             11834..12697
                     /locus_tag="WJK66_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEZ7578643.1"
                     /translation="MRKITFIIPIKQSLIKPDCFIRLFFNLFLLKKFSSKYGFSILVA
                     DNSNFLWKNIIKLITKFYKCNYISIKSHNTFYTPAKIKNAAAIYSFNTLNSNYILFLD
                     VDVLLSENFIQHLIKKTKTNIAFDWYPVSFLNKQFGIINFILFSYKGNLNIEESFIIQ
                     TGFVTGLQLFNSDFFYKTAGYNESFLGYGCEDIEMIHRATLLLNIRPAFNENHQYFTD
                     DRGYMPSKLTGFRNYFYYLKRDEFSNLQITPKHFWHKRKNKSKYLKNRYQNDVKMIQI
                     MKDFDRKFLKN"
     gene            12742..>13616
                     /gene="galE"
                     /locus_tag="WJK66_000913"
     CDS             12742..>13616
                     /gene="galE"
                     /locus_tag="WJK66_000913"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEZ7578644.1"
                     /translation="MKNILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPR
                     LKTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPMKYYDNNV
                     SGSLVLAEEMARAGVFSIVFSSSATVYGDPGKVPYTEDMPLGDTTSPYGTSKSMVERI
                     LTDIQKADPRWSMILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAAGKLPQLAV
                     FGDDYPTPDGTGMRDYIHVMDLAEGHVAAMQAKSNVAGTHLLNLGSGRASSVLEIIRA
                     FEAASGLTIPFEIKPR"
BASE COUNT         4057 a         3069 c         2701 g         3789 t
ORIGIN      
        1 cggggttgga tttgataggg aatatttaag ccggatgcgg cctcaaaggc acggatgatt
       61 tccaaaacgg aataggcgcg gcccgaaccc aagttgaaca aatgtacgcc ggaaaggccg
      121 cctttcgcct tcattgccgc gatatgccct tctgccaaat ccatcacatg aatgtagtcg
      181 cgcattcccg taccgtccgg ggtcggataa tcgccgccga ataccgacag ttgcggcagc
      241 ctgcccgaag ccacttggca gatatagggc aaaaggttgt tgggaatacc gttgggctgt
      301 tccccgatca agccgctttc gtgcgcgccg atcgggttga aatagcgcaa caaaatcacg
      361 ctccaacgcg ggtcggcttt ttggatgtcg gttaacatcc gttccaccat cgctttggat
      421 gcgccataag gattagcggt atcgcccggg cgcatatctt ccgtataggg cattttacgt
      481 atcatggcgc gacgcattaa agtaaacgcc aaagcgcgtt tatactggtt atccagtttc
      541 cctatatttt tacgttacaa atcaagttgt tggtattcat cgtgaaaata gaatttttcg
      601 gcataacgct gactcataaa accggcatgc gggctgcctg aaaaaatcat tgcgacatca
      661 tcgtcattta atgtttcatg gttgttttct attaatgcta aagcaatttg gtaattaatt
      721 gccaaaatat cgttagtaat cacacgatag ccgcgttttt tggcttcgta ggcaaaggaa
      781 cagcctccac taaatacatc tgcaactgta tctacatcag acggaagctg gtcacaaatc
      841 taggaagcta ttttctcttt attaccgata tagttaattt tcggatattg cttattcatt
      901 ttcactcatt tttaaaatac tttcagcaat agcttttgcc aataaaggcg gtacggcgtt
      961 accaacctgc tgctgctggg caattttgct gccgcaaaaa ataaaattat cagggaaaga
     1021 ttgtaaggca gctaattcac gaacggttaa cgcccgattc tgttcatagt gaaaaacttt
     1081 gcgcatatct cctgtaatac aaacggctgg ttttgttgct gttgtaacgg atgtatttac
     1141 ggatatcacc tgttttcgga cgtaatggtt caggaatatc gttacggtta cctccatttt
     1201 taacaaatgc cattttttct aacatttgtg ccgaatgatt catagcttca tgatttgcaa
     1261 cgtgtggatt gctttcgcca gcagccagtt ttggaaaatg tcctattgct gatccaacag
     1321 tctgatggga aatctgcaaa ggttcgggaa aggaaatttt gcctttatcc ctcctcccga
     1381 taaatatcac tcggctacgt atctgaggaa caccgaaatc ggctgcactc agtatcttac
     1441 attccaccga ataaccgata ttctgaaatg cttgaataat ctcaatacgt gttttacctg
     1501 aattgtgtgt atagagtcgc gctacatttt ccataacaaa aaaatatggt tggacaattt
     1561 taactattcg gacaaactct ttaaataaat ggttgcgtgg gtcatctgta aatgtccgtc
     1621 caatctttcc tgccatacta aaaccttgac aaggtggtcc tccaataatc aaatcaactg
     1681 cttgtccgtt aagacaattg attaaatctt gttcggttag tgtggttaaa tctttttgca
     1741 gtaattgatg atgggggaag ttggtacggt aagtctgaca ataatcagac tccatttcaa
     1801 cagaaagcaa ttgttggaat ccggcttgtt caaaacccaa ggataggcct cctgctcctg
     1861 agaaaaggtc aatataggta agtggtgtgt gcataagtca aaatccataa actcttcgat
     1921 tattttaccg cttcagcttc gcaaacgcat ccgccatcgc cgaattggct ggggcgcggt
     1981 cgttgcgttg gggtttgcgg ccggctgtcc gttcgcggct gcggttttca gacggcattt
     2041 tatgttttgc gccgcccggt tcgtcatcca agcgcatggt cagcgcgatg cgtttgcgtg
     2101 cagcatcgac ttccagcact ttcactttca ccacgtcgcc agctttcacc acttcgcgcg
     2161 ggtcttggac gaacttgttg gacagggcgg agatgtgcac caagccgtcc tgatggacgc
     2221 cgatgtccac gaacgcgccg aagttggcga cgttggaaac cacgccttcg agtatcatac
     2281 cgacttgcaa gtcgctgatt tcgtggatac cttcggcaaa cgatgccgtc tgaaactcgc
     2341 cgcgcggatc acggccgggt ttttccagtt cggacaggat gtccaaaatg gtcggcaggc
     2401 cgaagcgttc gtcggtgaag tcggacactt tgatttgctt cacgcgctcg cggttgccga
     2461 tgagttcggc ggcgctaatg ccttgttgcg ccagcatttt ggcgacgacg ggataggctt
     2521 cggggtggac ggcgctcgcg tccaacggct ctttaccgcc gttaatccgc aaaaagcctg
     2581 ccgcctgctc gaaggttttt tcgcccaaac gcggcacttt cagcaatttt ttgcggctgt
     2641 cgaacgcgcc gttttcatcg cggtaggcga cgatgttttg ggcaagagtt tgattcaaac
     2701 cagaaatccg cgccaagagc ggggcggagg cggtattcac gtccacgccg acggcgttca
     2761 cgcagtcttc gaccaccgcg tccagcgatt tggcgagccg gctttggttc acgtcgtgct
     2821 gatactggcc cacgccgatg gatttggggt cgattttgac cagctcggca agcgggtctt
     2881 gcagcctgcg ggcgatggac accgcgccgc gcagggaaac gtccaagtcg gggaactcgc
     2941 gcgctgccag ttcggacgcg gaataaatcg acgcgccggc ttcggacacg acgattttat
     3001 gcagccccat tttcgacatt tcacgcacca gttcgcccgc gattttgtcg gtttcacggc
     3061 tggcggtgcc gttaccgatg gcgatgagct tcacgccgtg ctgtttaatc aggcgcgaca
     3121 gcgttgccaa catattgttt tcttgatgca aatagacgat gacggtatcc agcagcttgc
     3181 cggtgtcgtc caccacggcg catttcacgc cgttgcggta gccggggtcg agacccagcg
     3241 tggtcagccg tccggcgggc gcagcaagca gcaagtcttt gagattgcgg gcgaacacgg
     3301 taatcgcgtc ggtgtcggcg gcttctttca aacgattgag agcttcaagt tccaacgaca
     3361 aaaagatttt cgcgcgccaa gtcaggcgca cggtgtcgcg cagccatttg tggccgtctg
     3421 aaactttgaa gcggcgggcg atgatttgct cgtattcgct ttgctgcgta atcggcgtgt
     3481 cgtcgggctg atatttgagc gcgatgttca acacgccttc gttgcgtccg cgcaaaaccg
     3541 ctagcgcacg atggctgggc atcgcacgaa taggttcgcg gtggtcaaaa taatcgctga
     3601 atttttcgcc ttcggtttct ttgcctccaa caacttgcgc gtggatttcg gcttcgttcc
     3661 acagcttgtc gcgcagcgtg ccgatgagtt ccgcgtcttc ggcaaactgc tccatcagaa
     3721 tcgcacgcgc gccgtccaac gcggctttgg catcggggac gttttcgttc aggtagcctt
     3781 gtgcggcggc ttccacgtcc tgcgactgct cggcaagcaa cacgtccgcc agcggctgca
     3841 aaccgtgttc gcgcgcgatt tgcgctttgg tgcggcgttt gggtttgtag ggcagataca
     3901 ggtcttccag cgcggttttg ttatcggcgg cttcgatttg cgccctgagg tcgtctgaaa
     3961 gcttgccttg ctcttcaatg ctttttaaaa caacggcttt gcgctcttcc aactcgcgca
     4021 gatattgcag ccgctcggca agccggcgca actgcgtatc gtccaacccg cccgtcgctt
     4081 ccttgcggta gcgggcgata aacggcacgg tcgcgccgtc gtccaaaagc tcgacggcgg
     4141 cggtgatttg cgcggcagtc gcggagagtt cttgggaaag aatttgagta atgttcatca
     4201 ataaaattcc aacggacagg ccgtctgaaa tttcagacgg cctgatttaa aaacaatcgc
     4261 tttaaggcag cgaattataa tattcgtagg ctttgtccat atcttcaaac tggtacatat
     4321 gccctttttc cagcaccatc gcattatcgc aatattgctt catggcgctg tggctgtgcg
     4381 acaccaagat gatggaacgg tctttgcgct tttcaaacaa ctcgtactta catttatcgg
     4441 caaaacgcga gtcaccaact gcaatcactt cgtcaatcaa atagcaatca aactctaccg
     4501 ccaacgacaa agcaaatgcc aaacgcgcct tcatacctga agaataacgc ttgaccggct
     4561 cgtaaagata ttgccccaac tcggaaaact cttcggtaaa gttcttcaca taatcgatat
     4621 cgacattgta aatccggcag atgaaacgca aattgtccat accggtcaga ctgccttgaa
     4681 acgcaccgga gaatgccaaa ggccaagaaa tactcattgt ccgcttgatt tcacccgtgg
     4741 tcggcggctc aacgccactg atcaaacgga tgagcgtcga tttacctgca ccgttgcggc
     4801 cgagaatacc gattttctcg cccttctcca ttttgaagct aatatcgtgc aagactgtcc
     4861 gccaaccttg gcgggtcaga tagcgtttgg aaacgtgttc aactgaaatc attgcggctc
     4921 aacccctttg ctgaatctcg ccaccaccgc caaaccaagc agcagcaaaa ccaagttaca
     4981 caacaaaata taccaaggat tttcgtaagt cgtgacgctg tcgccaaaat atccggcacg
     5041 gaacatttct gtaccatgca ccatcggaat catcagcaca tagtgttgca actgctgagg
     5101 caggttgtgt acgaagaaaa acacgccgga caaaggcatc atcacaaaac tgatggtact
     5161 ccaaactttg ccaaacggtt caaaatgaaa cgccaccgaa cagatgacca gccccaatcc
     5221 aacggcaaac atagccatca aaagccaagc caacaacata tagaaaatat ctgccggtac
     5281 atcaatccaa ccgatgataa ccaatgcgaa cataatcacg acctgcgcaa tggtggcgcc
     5341 ggcaatctcc aacaacatac gcgcaaaaat ggtatccaat acgcgtacat ttcggtgata
     5401 cagcaggctt gtgttggcag aaatcgaacc gatagcatga ttagaagcat tacgccacat
     5461 catcatcatc ggatagcctg tcagcgtgaa tgccacgata ttcaaagctg acacgttgtg
     5521 catacggaaa aacttccaca tcaacaccat aaccaaagtc agcagcaaag gttcgacaaa
     5581 caaccacaaa aaaccaatgt tattacgacc gtagcgagta ataatttcac gcattaagag
     5641 tgcgccgatt accctttttt ggataagcag cgactccaaa aacgatgttt tatgtagctc
     5701 tttcatcagt ttttatgctc acggatactt gcagacagca acacgacaat gccatagaca
     5761 atcaaaccga tgacaaacgt tgccacgatg ttgtacaaac ggtttggctc atatgccaag
     5821 tcaggtttgt tcggctggga aatcacttcc aaatacaatt gctgacgatc tgcttccgct
     5881 tttgcacttt ccaaagaagt catcgccgca gccaattgtt tttcggccaa ttcgttttcc
     5941 aaatacacgc gttgatattc cgccgcctga ttggacaacg aaccctcgcc accgccggaa
     6001 atcgctttca tttgttgcga gatttcttta cgcaaactct tctcacgcgc aatcaaacct
     6061 ggaatttgcg ggttttcagg cgtaacggct tttacctggt ccagctgggt ctgaatgaca
     6121 atcaactcgt cctgtaattt ggataccaaa cccatttgca catcggattg cgccttcaag
     6181 tcgaaaatac cgttagacac acggaatttt gtcaattgga cagaagcctc tttaacttgc
     6241 tcttcagcag aattgaccac ttcctgagca taacgaatcg tatcctgacg cgcacgttcg
     6301 ttcagctggt taatcaatac ctcaccctgt ttaagcaaag catcattgat cttttgagat
     6361 tcaccggcat taaacgatgt aacgctcaaa ttggaaatgc ctgagacaga gtcaaaatgg
     6421 atggatacct tatcacggta gtattgataa aacgcctcat cctcgccacg caggccaaaa
     6481 ccattaaaac ggctgaaaat atcgccttct ttttcataaa aatcgcgaat gggcattttc
     6541 ttacgcaacg catccaaagc cgaacgcgac tgcatatatt cctgcacggt ataaatatcg
     6601 tcttgcgcac gggcaaaacc tgtgccctgc aaaatggcac ccaggccatt gagagaagat
     6661 tggcttttag gcgagcgcac cacaaagctc gattgcgacg taaaacgatc ggaagcgaag
     6721 aagccgaaat acaccaacga aattaccgta gggataatca ccgttaccca aaataaaggg
     6781 cttagctttt taatccaact tttctttttc ggctttttac gctcggcttt ggtttcagtg
     6841 gcaactgcca caggaagttg ctcagacatc ttaattactc acattaatta gttaaattat
     6901 taatactgtt cgcgccactg gtaaccggcg agaacacaaa cgacaagaat ttctgcactt
     6961 cagccaacgg cgcattcgac acatacaata catctttatt cttcacagga aagcgctgca
     7021 tagaaaatag cgaatgcgca tcagccatat tcacacgata taccgttgga atctctgcct
     7081 cactgccata accttgagca atccatttat cctgacgttc tgccggcaat tccaccaatg
     7141 gcgtatagcg gaacacaaac acaccacgcg catcagaacg gcgatcttgc aaaccgccca
     7201 tacggccaat ggcttcagaa agcgataagc ctctggctga aaaaccgatt tcttgtgttc
     7261 tccccaccgc acccatagac gtaaaggtat agggattggt aatcatggta accacatcac
     7321 cgcgacgcag caaaatattt tgtcgcggat ttgcaactaa atcttccaag gcaacagttc
     7381 gtactacatt gccacgtgtc agctgcacat tcgtatcctg cacatttgcc gttgaaccac
     7441 ctaccgcagc caccgcatcc aacacacgct caccggctgc cgtcaacggc atacgcacac
     7501 tattgcctgc gcgaatcacc gatacatttg ccgcattatt ctgcaccaag cgcaccatca
     7561 cttgcggctg attggccatt tttttcaggc ggcctttaat aatttcctga acctgaccag
     7621 gcgttttacc gaccaccgaa atatcgccaa caaacggcac agaaaccgta ccacgtgccg
     7681 tcaccagttg ctccggcaac ttggtttgtt gcgcactacc cgagcccatc gaagaaaggc
     7741 caccaccaaa caataccgct ggcggcgctt cccaaatcat aatatccaat acatcaccaa
     7801 tatttagcgt accggtagaa gcataaccat cgccaaactg ggtaaaagac tgattcacct
     7861 gagctttata caaagactgc gctacagcat gattgacgtc aatcaactct acttcaggaa
     7921 tttgagcttc agactgttgc cccaatgaga caactttttt tgcacttggg ccagaggagg
     7981 gaatagcact acatgcactt cccaaaatta aaaaagaaat tacaatacaa aactttaact
     8041 taagcataaa atctcattaa gtatgattgt ttttaaataa atttaaaacc taccagagat
     8101 acaataccac tttattttgt agaacacaaa cgtgtataat atataacata aacatcatct
     8161 tcgaaataat attggggctt aggaagcaaa atcatcaaaa aacgtgataa gctcctaata
     8221 tttttaacac attactatat tacacatagg atattccaat gaaagtctta accgtctttg
     8281 gcactcgccc tgaagctatt aaaatggcgc ctgtaattct agagttacaa aaacataaca
     8341 caattacttc aaaagtttgc attactgcac agcatcgtga aatgctagat caggttttga
     8401 gcctattcga aatcaaagct gattatgatt taaatatcat gaaacccaac cagagcctac
     8461 aagaaatcac aacaaatatc atctcaagcc ttaccgatgt tcttgaagat ttcaaacctg
     8521 actgcgtcct tgttcacgga gacaccacaa caacttttgc agctagcctt gctgcattct
     8581 atcaaaaaat acctgttggc cacattgaag caggcctgag aacttataat ttatactctc
     8641 cttggccaga ggaagcaaat aggcgtttaa caagcgttct aagccagtgg cattttgcac
     8701 ctactgaaga ttctaaaaat aacttactat ctgaatcaat accttctgac aaagttattg
     8761 ttactggaaa tactgtcata gatgcactaa tggtatctct agaaaaacta aaaataacta
     8821 caattaaaaa acaaatggaa caagcttttc catttattca ggacaactct aaagtaattt
     8881 taattaccgc tcatagaaga gaaaatcatg gggaaggtat taaaaatatt ggactttcta
     8941 tcttagaatt agctaaaaaa tacccaacat tctcttttgt gattccgctc catttaaatc
     9001 ctaacgttag aaaaccaatt caagatttat tatcctctgt gcacaatgtt catcttattg
     9061 agccacaaga atacttacca ttcgtatatt taatgtctaa aagccatata atattaagtg
     9121 attcaggcgg catacaagaa gaagctccat ccctaggaaa accagttctt gtattaagag
     9181 atactacaga acgtcctgaa gctgtagctg caggaactgt aaaattagta ggttctgaaa
     9241 ctcaaaatat tattgagagc tttacacaac taattgaata ccctgaatat tatgaaaaaa
     9301 tggctaatat tgaaaaccct tacgggatag gtaatgcctc aaaaatcatt gtagaaactt
     9361 tattaaagaa tagataaaat gtttatactt aataacagaa aatggcgtaa acttaaaaga
     9421 gaccctagcg ctttctttcg agatagtaaa tttaactttt taagatattt ttctgctaaa
     9481 aaatttgcaa agaattttaa aaattcatca catatccata aaactaatat aagtaaagct
     9541 caatcaaata tttcttcaac cttaaaacaa aatcggaaac aagatatgtt aattcctatt
     9601 aattttttta attttgaata tatagttaaa aaacttaaca atcaaaacgc aataggtgta
     9661 tatattcttc cttctaatct tactcttaag cctgcattat gtattctaga atcacataaa
     9721 gaagactttt taaataaatt tcttcttact atttcctctg aaaatttaaa gcttcaatac
     9781 aaatttaatg gacaaataaa aaatcctaag tccgtaaatg aaatttggac agatttattt
     9841 agcattgctc atgttgacat gaaactcagc acagatagaa ctttaagttc atctatatct
     9901 caattttggt tcagattaga gttctgtaaa gaagataagg attttatctt atttcctaca
     9961 gctaacagat attctagaaa actttggaag cactctatta aaaataatca attatttaaa
    10021 gaaggcatac gaaactattc agaaatatct tcattaccct atgaagaaga tcataatttt
    10081 gatattgatt tagtatttac ttgggtcaac tcagaagata agaattggca agagttatat
    10141 aaaaaatata agcccgactt taatagcgat gcaaccagta catcgagatt ccttagtaga
    10201 gatgaattaa aattcgcatt acgctcttgg gaaatgaatg gatccttcat tcgaaaaatt
    10261 tttattgtct ctaattgtgc tcccccagca tggctagatt taaataaccc taaaattcaa
    10321 tgggtatatc acgaagaaat tatgccacaa agtgcccttc ctacttttag ctcacatgct
    10381 attgaaacca gcttgcacca tataccagga attagtaact attttattta cagcaatgac
    10441 gacttcctat taactaaacc attgaataaa gacaatttct tctattcgaa tggtattgca
    10501 aagttaagat tagaagcatg gggaaatgtt aatggtgaat gtactgaagg agaacctgac
    10561 tacttaaatg gtgctcgcaa tgcgaacact ctcttagaaa aggaatttaa aaaatttact
    10621 actaaactac atactcactc ccctcaatcc atgagaactg atattttatt tgagatggaa
    10681 aaaaaatatc cagaagagtt taatagaaca ctacataata aattccgatc tttagatgat
    10741 attgcagtaa cgggctatct ctatcatcat tatgccctac tctctggacg agcactacaa
    10801 agttctgaca agacggaact tgtacagcaa aatcatgatt tcaaaaagaa actaaataat
    10861 gtagtgacct taactaaaga aaggaatttt gacaaacttc ctttgagcgt atgtatcaac
    10921 gatggtgctg atagtcactt gaatgaagaa tggaatgttc aagttattaa gttcttagaa
    10981 actcttttcc cattaccatc atcatttgag aaataagtta aattatgaag aacctttgag
    11041 tgcaattcga aggttcttca ttcatattat tcatattttg gagaaattat gttatctaat
    11101 ttaaaaacag gaaataatat cttaggatta cctgaatttg agttgaatgg ctgccgattc
    11161 ttatataaaa aaggtataga aaaaacaatt attacttttt cagcatttcc tcctaaagat
    11221 attgctcaaa aatataatta tataaaagat tttttaagtt ctaattatac ttttttagca
    11281 ttcttagata ccaaatatcc agaagatgat gctagaggca cttattacat tactaatgag
    11341 ttagataatg gatatttaca aaccatacat tgtattattc aattattatc gaatacaaat
    11401 caagaagata cctacctttt gggttcaagt aaaggtggcg ttggcgcact tctactcggt
    11461 cttacatata attatcctaa tataattatt aatgctcctc aagccaaatt agcagattat
    11521 atcaaaacac gctcgaaaac cattctttca tatatgcttg gaacctctaa aagatttcaa
    11581 gatattaatt acgattatat caatgacttc ttactatcta aaattaagac ttgcgactcc
    11641 tcacttaaat ggaatattca tataacttgc ggaaaagatg attcatatca tttaaatgaa
    11701 ttagaaattc taaaaaatga atttaatata aaagctatta cgattaaaac caaactaatt
    11761 tctggcgggc atgataatga agcaattgcc cactatagag aatactttaa aaccataatc
    11821 caaaatatat aaaatgcgta agattacttt tattatccct ataaaacagt ctttaataaa
    11881 acctgattgc tttatacgcc tcttttttaa tttatttttg ctaaaaaaat tctcaagtaa
    11941 atacggattt tctatattag ttgcagacaa cagtaacttc ctttggaaaa atattattaa
    12001 attaattaca aaattttaca aatgtaatta tattagtatt aaatctcata atacttttta
    12061 tacgcctgct aaaattaaaa atgcagctgc catctatagt tttaatacct tgaattcaaa
    12121 ttacatttta ttcttagatg ttgacgtttt attatcggaa aattttattc aacatttaat
    12181 aaaaaaaaca aaaaccaata tcgcctttga ttggtaccct gtttcattct taaacaaaca
    12241 atttgggatt ataaatttta tattattctc atataaaggt aatctaaata tagaagaatc
    12301 attcattata caaacagggt ttgtaactgg cttacaatta tttaattctg attttttcta
    12361 caaaacagct ggatacaatg aaagctttct tggctatggc tgtgaagata ttgaaatgat
    12421 tcacagagca acattattat taaatattag acctgccttt aatgaaaatc atcaatattt
    12481 tacagatgat agaggatata tgccttctaa attaaccgga tttcgaaatt atttttatta
    12541 tttgaaaaga gatgaatttt caaacttaca gataactcct aaacatttct ggcataagcg
    12601 aaaaaataaa tcaaaatatc taaaaaatag atatcaaaat gatgtaaaaa tgattcagat
    12661 tatgaaagat tttgatcgaa aatttctaaa aaattaacga gctgtcttgc ccatatgaat
    12721 cctgattact ttaatttaat tatgaaaaat attctcgtta ccggcggcac cggttttatc
    12781 ggctcgcaca ccgttgtttc tttgctgaaa agcggccatc aagtcgtgat tttggataac
    12841 ctatgcaatt ccagcatcaa tatcctgcca cgcttgaaaa cgattaccgg ccaagagatt
    12901 ccgttttatc aaggcgatat ccgcgatcgt gagattttgc gccgtatttt tgcggaaaac
    12961 cgcattgatt cggtgattca ttttgccggc ttgaaagcgg tgggtgaaag tgtggccgag
    13021 ccgatgaaat attatgataa taatgtttcc ggcagcttgg tgttggcgga agaaatggcg
    13081 cgtgcgggcg tgtttagcat tgtgttcagt tcttcggcga cggtttatgg cgatccgggc
    13141 aaagtgcctt ataccgagga tatgccactg ggcgatacca ccagccctta cggcacatcg
    13201 aaatcaatgg ttgagcgcat tctcactgac attcaaaaag ccgatccgcg ctggagcatg
    13261 attttgttgc gttatttcaa tccaattggc gcgcatgaaa gcggcttgat tggcgagcag
    13321 ccaaacggta tcccaaataa tttgttgcct tatatctgcc aagtggcggc aggcaaactg
    13381 ccgcaattag cggtattcgg cgatgactac cctacccctg atggcacggg tatgcgcgac
    13441 tatattcatg tgatggactt ggctgaaggc catgtcgccg ccatgcaggc gaaaagcaat
    13501 gtagcaggca cgcatttgtt gaacttaggt tccggtcgcg cttcttcggt attggaaatc
    13561 atccgcgcat ttgaagcggc ttcgggttta accattccat ttgaaatcaa accacg
//
LOCUS       DAWKOG010000042        13416 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.042, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000042 DAWKOG010000000
VERSION     DAWKOG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13416
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.042"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(121..1185)
                     /locus_tag="WJK66_000914"
     CDS             complement(121..1185)
                     /locus_tag="WJK66_000914"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HEZ7578645.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLMEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMANRYAKGELNQIVK"
     gene            1379..2296
                     /gene="xerC"
                     /locus_tag="WJK66_000915"
     CDS             1379..2296
                     /gene="xerC"
                     /locus_tag="WJK66_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEZ7578646.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEAEGGVPQDLSRRDFTAALRRLSQRGLNARTLARKLSSWRQYCVWLVERGLLHTDP
                     AADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            2381..4294
                     /gene="dxs"
                     /locus_tag="WJK66_000916"
     CDS             2381..4294
                     /gene="dxs"
                     /locus_tag="WJK66_000916"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEZ7578647.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRSESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVAKLPKEGAAQMPSEKEPKPVAKPTYTQ
                     VFGKWLCDRAAADERLIAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCIPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGGKTAFIAFGSMVAPALAVAGKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDDLGLSAEAVER
                     RVRAWLSDRDAAN"
     gene            complement(4379..5707)
                     /gene="miaB"
                     /locus_tag="WJK66_000917"
     CDS             complement(4379..5707)
                     /gene="miaB"
                     /locus_tag="WJK66_000917"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEZ7578648.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLHDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKVVEA"
     gene            5910..6323
                     /locus_tag="WJK66_000918"
     CDS             5910..6323
                     /locus_tag="WJK66_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ7578649.1"
                     /translation="MKPAVWAALLLCACTSNFGDREHQFLRDQTEEKIVQFAVRGKTT
                     RAEVEARFGRQNPFGCYAYHEVSLPIYNFLPTNFIYMKSERRHWEWCVDYDGEGVVRD
                     YRFTHKVEKDERSVIRDAVGVIRKEAGKSLSQPEK"
     gene            6387..7670
                     /gene="hemL"
                     /locus_tag="WJK66_000919"
     CDS             6387..7670
                     /gene="hemL"
                     /locus_tag="WJK66_000919"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEZ7578650.1"
                     /translation="MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVVEAVREAALGGLSFGAPTEGEIAIAEQIAKI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPSEA
                     FVKALRELTEKHGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIRREGFYENLTARTEQLVQG
                     FQTAADAAGIEFAADSVGGMFGLYFASHTPRNYADMARSNIDAFKRFFHGMLDRGIAF
                     GPSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAA"
     gene            complement(8167..8730)
                     /gene="orn"
                     /locus_tag="WJK66_000920"
     CDS             complement(8167..8730)
                     /gene="orn"
                     /locus_tag="WJK66_000920"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEZ7578651.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAVHQSDELLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWVPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
     gene            complement(8748..9635)
                     /gene="prmA"
                     /locus_tag="WJK66_000921"
     CDS             complement(8748..9635)
                     /gene="prmA"
                     /locus_tag="WJK66_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEZ7578652.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQIAAQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEVPEGSAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGKDNAEQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETEESWARLSGTKR"
     gene            complement(9753..11114)
                     /gene="accC"
                     /locus_tag="WJK66_000922"
     CDS             complement(9753..11114)
                     /gene="accC"
                     /locus_tag="WJK66_000922"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEZ7578653.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAIYLAERDCSMQRRHQKVIEEAPAPFITEKERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIAAGLPLQYKQKDIQVEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
     gene            complement(11227..11652)
                     /locus_tag="WJK66_000923"
     CDS             complement(11227..11652)
                     /locus_tag="WJK66_000923"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEZ7578654.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPAVTPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQQVKAGDTLCI
                     IEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            complement(11634..11891)
                     /locus_tag="WJK66_000924"
     CDS             complement(11634..11891)
                     /locus_tag="WJK66_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HEZ7578655.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            12008..13048
                     /gene="queA"
                     /locus_tag="WJK66_000925"
     CDS             12008..13048
                     /gene="queA"
                     /locus_tag="WJK66_000925"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEZ7578656.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLHRLKDKGAVTAEVTLHVGAGTFQPVRVDKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATGRLKAGQGDTD
                     IFITPGYRFNVVDRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMVLGRNEGVVR"
BASE COUNT         2792 a         3428 c         4045 g         3151 t
ORIGIN      
        1 taaacatttt ggttatggta tctttttttg catactgcat caatgaggca ggtcaaagaa
       61 gcaaaaatca aatgccgtcc gaacaacggt tcagacggca ttttgtttac aagcaacctg
      121 ttatttgacg atttggttca attcgccctt ggcataacgg tttgccattt tttccaagga
      181 aaccggtttg attttgcctg cctgaccttc gcaaccgaac gcgaggtagc ggtcgaggca
      241 gatttgcttc atcgcttcaa tggttttgct caaatatttg cgcggatcga agtcggacgg
      301 gttttcggca aggtagcggc gtaccgcgcc ggtggaagca aggcgcaagt cggtatcgat
      361 gttgacttta cgcacgccgt gtttgatgcc ttcgacgatt tcttcaaccg gcacgccgta
      421 ggtttcgccg atattgccgc cgtattcgtt gatgactttc agccattctt gcggaacgga
      481 gctggagccg tgcatcacga tgtgtgtatt gggcagggct tggtggattt ctttgatgcg
      541 gtcgatacgc aacacgtcgc ctgtgggcgg acgggtgaat ttgtacgcgc cgtggctggt
      601 gccgacggca atcgccaatg cgtcaacgcc ggtatcttta acgaaacgca cggcatcttc
      661 gacgctggtg agcatttggt cgtgggaaag tttgcccact gcgcctacac cgtcttcttc
      721 gccggcttcg ccagtttcga ggttgcccaa tacgccgatt tcgccttcaa cggatacgcc
      781 gcaggcgtgg gagaaattaa ccacggtacg ggtggcgttg acgttgtatt cataagaaga
      841 aggggttttg ccgtcttcca tcagcgagcc gtccatcatc acggaggaaa agcccagttg
      901 gatggagcgt tggcacacgt cgggcgatgc gccgtggtct tggtgcatca cgacggggat
      961 gtgcggaaat tcttcgacag ccgccaaaat caggtggcgc aaaaacggcg cgcccgcgta
     1021 tttgcgcgca cctgcgctcg cctgtacgat gacgggcgcg ttgacttggt cggcggcttc
     1081 cataatggcg cgcatttgtt cgaggttgtt gacgttgaac gcgggcaggc cgtagctgtt
     1141 ttcggcagca tgatcaagca gttggcgcat ggatacgagt gccatttgtg tctccttggg
     1201 caataggtaa ataaggcgga ttataatgtt ttttgcggca aaaaaccata aacggctcat
     1261 tgattttatg ttaacgataa cggcgggcgg acgggtcgga ttttgggcgg ttcggggttt
     1321 tggtgtgtcg tttatgataa tgttttcact gtttttttga tatttgtgtg gggcggttat
     1381 ggttttggac gggtttgcgg cgcattttga cgcttatttg gaaaacatcg tgcgcgaggg
     1441 caagtcggag cacacagtgg cggcataccg gcgcgatttg gaagaactgt ttgcgctgtt
     1501 ggcacaaatg ccgtctgaag ctgaaggcgg cgtgccgcag gacttgtcgc ggcgcgattt
     1561 tacggcggcg ttgcggcggc tgtcgcagcg cggtttgaat gcgcggacgc tggcgcgcaa
     1621 gctgtcgtct tggcggcagt attgtgtttg gctggtggaa cgcggtctgc tgcataccga
     1681 cccggccgcc gacatcaaac cgccgaagca gcccgagcgc gtacccaaag ccctgccgca
     1741 ggaatggctg aaccggatgc tggatttgcc cgtggacggc ggcgacccgc tggcggtgcg
     1801 cgaccacgct ctgttcgagc tgatgtacgg cagcggtttg cgcgtgagcg agatacacgg
     1861 cttgaatgca gatgatgtct atttggacga ggcgtgggta cacgttaccg gcaaagggcg
     1921 caagcagcgg caggtgccgc tgaccggcaa aagcgtggaa gccctgaaaa actatctgcc
     1981 gctgcgtcag acggcatcgg acggcaaagc cctgtttacc ggcaggaacg gcacgcgcct
     2041 gagccaacgc caaatccaaa aacgtttggc gcaatgggcg gcgcaaaacg gcgacggcag
     2101 gcacgtttcg ccgcatatga tgcgccacag ctacgccgga cacctgttgc aggcttcgcg
     2161 cgacatcagg gcggtgcagg agctgctcgg gcacagcagc ctttcgacca cgcagattta
     2221 taccaagctc gatttcgacc acatcgcccg cctgtatgac gaagcccacc cgcgcgccaa
     2281 gcggcaggac gaatgacata cggcaaaatc agccgtcagc cgcacgctct tgatatataa
     2341 ttggccgttg cacccggacg acacataaaa aagacacacc atgaacccaa gccccctact
     2401 cgacctgatt gacagcccgc aagatttgcg ccgtctggac aaaaaacagc tgccgcgcct
     2461 tgccggcgag ttgcgcgcct ttctgctgga atctgtcggg cagaccggcg ggcatttcgc
     2521 cagcaatttg ggcgcggtcg aactgaccat cgccctgcac tatgtgtacg atacgcccga
     2581 agacaagctg gtatgggatg tcggacacca aagctatccg cacaaaatcc tgacaggcag
     2641 gaaaaaccag atgcacacca tgcgccaata cggcggttta gctggcttcc caaaacgcag
     2701 cgagtcggaa tacgacgcgt tcggcgtagg gcattcttcc acatccatcg gcgcggcgtt
     2761 gggcatggcg gcggcagaca aactcttggg cagcgaccgc cgcagcgtcg ccatcatcgg
     2821 cgacggcgcg atgacggcgg ggcaggcgtt tgaagccttg aattgcgcag gcgatatgga
     2881 tgtggatttg ctggtcgtcc tcaacgacaa cgaaatgtcg atttccccca acgtcggcgc
     2941 gctgccgaaa taccttgcca gcaacgtcgt gcgcgatatg cacggcctgt tgagtaccgt
     3001 caaagcgcaa acgggcaagg tattagacaa aatacccggc gcgatggagt ttgcccaaaa
     3061 agtcgaacac aaaatcaaaa cccttgccga agaagccgaa cacgccaaac agtcgctgtc
     3121 tttgtttgaa aacttcggct tccgctatac cggccccgtg gacggacaca acgtcgaaaa
     3181 tctggtggac gtattgaaag acttgcgcag ccgcaaaggc cctcagcttc tgcacgtcat
     3241 caccaaaaaa ggcaacggct acaaactcgc cgaaaacgac cccgtcaaat accacgccgt
     3301 cgccaagctg cctaaagaag gcgcggcgca aatgccgtct gaaaaagaac ccaagcccgt
     3361 cgccaaaccg acctataccc aagtgttcgg caaatggctg tgcgaccggg cggcggcaga
     3421 cgagcgtttg atcgccatca cgcccgcgat gcgcgagggc agcggactgg tggagtttga
     3481 acaacgattc cccgaccgtt atttcgatgt cggtattgcc gagcagcacg ccgttacctt
     3541 tgccggcggt ttggcgtgcg aaggcatgaa gcccgtcgtg gcgatttatt ccaccttttt
     3601 acaacgcgcc tacgaccaac tggtgcacga tatcgccctg caaaacctac ccgttttgtt
     3661 tgccgtcgac cgcgcgggca tcgtcggcgc ggacggcccg acccacgccg gtttgtacga
     3721 tttgagcttc ttgcgttgca ttccgaatat gattgtcgcc gcgccgagcg atgaaaacga
     3781 atgccgcctg ctgctttcga cctgctatca ggcggatgcg cccgccgccg tccgctatcc
     3841 gcgcggcacg ggtacgggcg cgccggtttc agacggcatg gaaaccgtgg aaatcggcaa
     3901 gggcattatc cgccgcgaag gtgggaaaac cgcattcatt gccttcggca gtatggtcgc
     3961 ccccgcattg gcggtcgccg gaaaactgaa cgccaccgtc gccgatatgc gctttgtcaa
     4021 accgatagac gaagagttga ttgtccgcct tgcccgaagc cacgaccgca tcgttaccct
     4081 tgaagaaaac gccgaacagg gcggcgcagg cggcgcggtg ctggaagtat tggcgaaaca
     4141 cggcatctgc aaacccgttt tgcttttggg cgttgccgat accgtaaccg gacacggcga
     4201 tccgaaaaaa cttttagacg atttgggctt gagtgccgaa gcggtggaac ggcgtgtgcg
     4261 cgcgtggctg tcggatcggg atgcggcaaa ttaagccgcc cggccgcgcc gtcgtctgcc
     4321 gatacaaggc aaaatgccgt ctgaaacgct tcagacggca ttttttcggc gtgagggttt
     4381 aggcttcgac aactttgccg cgcagggaga aggtgtaggc ctcggtgatt tccaaatcga
     4441 tcatttggtt aatcatgtcg ggcgtgccgg tgaagttgac gacgcggttg ttggcggtac
     4501 gggcttgcag ttggtcgggg tcttttttgg agatgccttc gaccaggcag cgttgaaccg
     4561 tgccgatcat ggtttggttg atgcgcgcgg tttcggcttc gatgacttcg ttcaaggctt
     4621 cgaggcggcg cactttttct tcgtgcggcg tgtcgtcgtg caggttggcg gcaggcgtgc
     4681 cggggcgcgg actgtaaata aacacgaagc tcaagtcgaa ggcaatgtct ttcaccagtt
     4741 tcaaggtttg ctcgaactcg cgttcggtct cgccggggaa gccgacgatg aaatcgctgc
     4801 tcaggcataa atcaggacgg atggcacgca gtttgcggat gatggatttg tattccaaag
     4861 cggtgtagcc gcgtttcatc gcgctcaata cgcggtcgga accgctttga atcggcaggt
     4921 gcaggtggga aaccaatttg ggcaggtcgc ggtagcactc gataatcgag tcggtaaact
     4981 cgcgcgggtg gctggtggtg aagcgcatac gttcgatgcc ggggatttcg tggacgatgc
     5041 gcagcagggt ggcgaagtcg cagatttcgc cgtcgtccat ttcgccgcga taggcgttga
     5101 cgttttgtcc caagaggttg atttctttca cgccttgctg ggcaaggttg gcgatttctg
     5161 tcaatacgtc gttgagcggg cgggagaatt cttcgccgcg cgtgtagggg acgacgcaga
     5221 atgagcagta tttggaacag ccttccataa tcgatacaaa tgccgcgccg ccttcgacgc
     5281 gggcgggcgg caggtggtcg aatttttcga tttcggggaa ggaaatatcg acttgcgaca
     5341 gcccgctggt ttctttgtcc acaatcattt tgggcaggcg gtgcagcgtt tgcgggccga
     5401 agaccacgtc aacataaggc gcgcgtttga tgatgttttc gccttcttgc gaggcgacgc
     5461 agccggcaac gccgatgatg aggccggggt ttttttcttt gagcggacgc acgcgcccca
     5521 aatcggagaa gactttttcc tgtgcttttt cgcgcacgga gcaggtgttg aacaagatga
     5581 tgtcggcttc gtcggcttgg gtaacctgtt cgatgccgcc gtgttcttcg gcgaggacgg
     5641 cgagcatttt gtcgctgtcg tattcgttca tctggcagcc gaaggtgcgg ataaatactt
     5701 ttttcatggt ttgtgtcttt ctcaggcagc cgtaatcgcg gggctgattg ttgttggaat
     5761 gaaaaaattt cagacggcat gacgatgccg tctgaaaatc ggtgcggatt atagcacgat
     5821 gtgggttttg taaacagaat attgttttaa aatatgaatt taatcggtcg ggacggctgt
     5881 ataatgtttg gctttaatgg gaggtgtgta tgaaaccggc tgtgtgggcg gcattgctgc
     5941 tgtgtgcgtg taccagcaat ttcggcgaca gggaacatca gttcctgcgt gatcagacgg
     6001 aagaaaaaat cgtgcagttt gcggttaggg gcaagacgac ccgggcggaa gtggaagcgc
     6061 gtttcgggcg gcaaaatccg ttcggctgtt atgcctatca cgaggtcagc ctgcctattt
     6121 ataatttttt gccgaccaat ttcatttata tgaaatcaga acgccggcat tgggagtggt
     6181 gtgtggatta cgacggggag ggagttgtcc gggactaccg ctttacacat aaagtggaaa
     6241 aagacgagcg ttccgtcatc cgggatgcgg ttggcgtcat ccgcaaagaa gcaggcaaat
     6301 ccttgtcgca acctgaaaaa tgataaaatg ggactttctg cttctaagcc cgaaacctgc
     6361 cgttcaggcg gcatttgagg ataaatatga accgtaacga aattttattc gaccgcgcca
     6421 aagccatcat ccccggcggc gtgaattccc ccgtccgcgc attcggcagc gtcggcggcg
     6481 tgccgcgctt catcaaaaaa gccgaaggcg cgtatgtttg ggacgaaaac ggcacgcgtt
     6541 ataccgacta tgtcggctct tgggggcctg cgattgtcgg acacgcgcac cccgaagtcg
     6601 tcgaagccgt gcgcgaagcc gcgttgggcg gtttgtcgtt cggcgcgccc accgaaggcg
     6661 aaatcgccat tgccgaacaa atcgccaaaa tcatgccgtc cgtcgaacgg ctgcgccttg
     6721 tcagctccgg taccgaagcc actatgaccg ccatccgtct ggcacgcggt tttaccggcc
     6781 gcgacaaaat catcaaattt gaaggctgct accacggcca ttccgacagc ctgttggtga
     6841 aagccggcag cggtctgctg acgttcggca acccgtcttc cgccggcgtg cctgccgact
     6901 ttaccaaaca cactttagtc ctcgagtaca acaatatcgc ccaactggaa gaagcctttg
     6961 ctcaaagcgg caatgacatt gcctgtgtga ttttggagcc ttttgtcggc aatatgaatc
     7021 tggttaggcc gtctgaagcc tttgtcaaag ccttgcgcga attgaccgaa aaacacggcg
     7081 cggtgttgat ttacgacgaa gtgatgaccg gtttccgcgt cgcgctcggc ggcgcacaat
     7141 cgctgcacgg catcacgccc gacctgacca cgatgggcaa agtcatcggc ggcggtatgc
     7201 cgcttgccgc gttcggcggg cgcaaagaca ttatggaatg tatttcccca ctgggcggcg
     7261 tgtatcaggc aggtacatta tcaggcaacc cgattgccgt cgccgccggt ttgaaaacgc
     7321 tggaaatcat ccgccgcgaa gggttttatg aaaacctgac ggcgcgtacc gaacagttgg
     7381 ttcaaggttt tcagacggca gcggatgcgg cgggcatcga gttcgccgcc gacagcgtgg
     7441 gcgggatgtt cggcctgtat tttgccagcc atacgccgcg aaactatgcc gatatggcgc
     7501 gatccaatat cgacgctttc aaacgcttct tccacggcat gctcgaccgc ggcattgcct
     7561 ttggcccgtc cgcttatgaa gcaggtttcg tttctgccgc gcatacgccc gagctgattg
     7621 acgaaacggt tgcggttgcg gttgaagtgt tcaaggcgat ggctgcatga tgttttgacg
     7681 gacagagttt ctctgttcga tttgtttggc agattgaagt aagaatgcac accgccgtca
     7741 ttcccgcgca ggcgggaatc cagacctttc agtttctgta atgattgaaa ataacgggcc
     7801 gaaatcagcc gcgcccggca ggaagccgcc gccacctgaa acaatgccgt ctgaagcctt
     7861 cagacggcat tttatcggaa gcgcatcaga aaaaacggat agcgacatat gcggtaaaag
     7921 caagggcaat tacatagata aaccccttta taaattcaaa agtctcatct atatcgctat
     7981 atttttcttt cagttcgtca gattttcgat aacgtttcat tttcaagacc acttcatgac
     8041 caacaagccc gaccttccgg attcccgcct gcgcgggaat gacgggcgtg tatatttttg
     8101 atttcaatct gctgtaaaaa tgccgtctga aatgcaaaca gcgtttcaga cggcattgtt
     8161 tgtttcttat tgcgcttcgg ctctcggggc ggaaatcaga aagtgttcgc ggtagtggcg
     8221 catttcttcg atgctctcca aaatgtcgtc caatgccttg tgcgaaccgc gtttgacgac
     8281 gcttttggca acgggcggat tccagcgttt ggcaagctct ttcagtgtgg aaacgtcaag
     8341 gttgcggtag tggaagtagt tttccagttt cggcatatat ttgaccataa aacgccggtc
     8401 ttggtggatg gaattgccgc acatcggcgt ggcgcgtccg ggtacccatt ccgacataaa
     8461 gtccagcagt ttctgttcga cttcggcttc ggtatgcgac gattcgcgta cgcgctgtgt
     8521 cagccccgtc ctgccgtgtg tggcggtgtt ccactcgtcc atattgtcga gcagctcgtc
     8581 actttggtgg acggcgtaaa cttcggattg cgccaataca ttcaaatccg agtcggtaat
     8641 aatcatcgcg acttcgataa tgcggtcggt ttcgggatta agccccgtca tttccatatc
     8701 gagccagcag aggttgtttt tgtcctgcac ggtgtttcct ttccgtttca gcgttttgtc
     8761 ccgctcaagc gcgcccagct ttcttcggtt tccgccgggt cgagatcgaa ccattggctg
     8821 taaatgccgc cgagttcttc ggcttgttcg tccaacaaac cggataacac gatgcggccg
     8881 ccttgtttgg tgcgggcggc gagcatttcg ccgagcatac gtaaagggtt ggcgaggatg
     8941 ttggcgacaa ctacgtcgaa ttgcccttga ggcagaccgt cgggcaggaa gaattgtgca
     9001 tcgacgttgt tctgttcggc gttgtccttg ccggcgcgga cggcttgttc gtcaatatcc
     9061 acgccgacgg cagaacctgc gccgagtttg agggcggcga tggtcaggat gcccgaaccg
     9121 cagccgtagt cgaggacgct ttcgccgttt ttgagttgcg tatccaacca tttgaggcag
     9181 aggcgcgtgg tcgggtggct gccggtgccg aaggcgagtc cggggtcgag gcggaggttg
     9241 acggcgctgc cttcggggac ttcgtgccaa gagggggtaa tccacaggcg gtcggaaatc
     9301 cggatggggt cgaattgcga ttgcgtgaga cgcacccagt cttggtcttc gatggtttcg
     9361 ccggtgtatg ccaagtcttt taacccgcat tcttgtgcgg cgatttggat gatggcggcg
     9421 gcttcgtcgt gttcgccgaa cagggcgatg actttgctct gctgccagat ttgttcggcg
     9481 ggcataccgg gttcgccgaa aatcgcctgt tcgttttgcg tgccggcgta ggcatcttcg
     9541 atggcggcgg agagtgcgcc gtgttccatc agcgcgtcgg cgaggcgttc ggcgacggca
     9601 tcgttgacgt tgacggtgat ttgttggtag ggcatagcgg gctttcttaa cagaccttcg
     9661 gaatagggtc gtactgtata aggggatggg tgtgtaacgg aaatgccgtc tgaaaacgga
     9721 caaactgttt cagacggcat atcggggacg gcttatttgt cctgtttggc tttgcgatct
     9781 tccagccagt gttccaaata gtggatgctg acgccgcctt tttggaaacc cgcatcggcg
     9841 aacaggtcgc ggtgaagcgg cgtattggtt ttgatgccgg ttaccgccag ctcggcgagt
     9901 gcgacgcgca ttttcgccat tgcctgttca cgcgttttgc cgtgtacgca gattttgccg
     9961 atcaggctgt cgtagtacgg tgggatgcgg tagccttggt aaatgtggct gtccacgcgg
    10021 ataccgaagc caccgggcag gtggcagctt tcaatcgggc ccgggctggg gatgaagttg
    10081 tacgggtctt cggcgttgat acggcactca aacgcgtggc cttcgacttg aatatccttt
    10141 tgtttgtatt gcaaaggcag gccggcggcg atgcggagtt gctcctgcac gatgtccacg
    10201 ccggtaatca gctcggtaac cggatgctca acctgaacgc gcgtattcat ctcgataaag
    10261 aaaaattcgc cgtcttcgta caggaattca aacgtacctg cgccacgata gccgatgcgt
    10321 ttgcaggcgt cggcacaggc gttgccgatt ttggcgcgtt ctttttcagt gatgaacgga
    10381 gccggtgctt cctcgatgac tttttggtgg cggcgctgca tggagcagtc gcgctcggcg
    10441 aggtagatgg cgttgccgtg ttcgtcggca atcacttgga tttcgacgtg gcgcggacgc
    10501 tgcaggtagc gctccatgta aaccatcggg ttgccgaatg ccgcgcctgc ttcggctttg
    10561 gtcatttcga cagactggag gaggtcttct tttttctcga caacgcgcat accgcgaccg
    10621 ccgccgccac cggaggcttt gataatgacg ggataaccga ctttatcggc gattttgagg
    10681 atttcttcgc cgtcgtccgg caatgcgccg tcagaaccgg ggacgcaggg tacgcctgcc
    10741 gctatcatcg cgtgtttggc ggagactttg tcgcccatca ggcggatggt gtcgggtttc
    10801 gggccgataa aggtaaagcc ggactgctcg acctgttcgg cgaaatcggc gttttcggca
    10861 aggaaaccgt agcccgggtg gacagcgtcc gcgcagctta cttcggcggc ggcgataatg
    10921 gcggggacgt taaggtagct ttgcgcggaa gcggcagggc cgatgcacac ggattcgtcg
    10981 gcgagtttga cgtgcaggct gtctttgtcg gcctcggaat gcacggcgac ggtggcaatg
    11041 cccatttcgc ggcaggcacg gagtacgcgt aatgcgattt cgcctcggtt ggcgattaaa
    11101 acttttttca gcatgatgac tgttcctgtg attccggcaa cccgtccgta aaaaaaaggt
    11161 gcgggaacgc cgaaggggga aagcatttca gacggcatcg gaagtttatg ccgtctgaaa
    11221 acaggattat ccgatgatga agagcggttc gccgaattcg acgggcgtac cgttttcgac
    11281 cagaatttct ttgaccgtgc cggatttttc ggcttcgatt tcgttcatca gcttcatcgc
    11341 ttcgatgatg cacagcgtgt cgccggcttt aacctgttgg ccgacttcga caaaaggcgc
    11401 ggcattcggg ccgggtgcgc ggtagaatgt gccgaccata ggcgattttt gcgcgtcgga
    11461 caaatcgcgg gcggcaggtg cggcggcagg cgttacggcc ggcgcggcgg caggtacggg
    11521 cgcggcgtaa acgggtgcgg cggcgatggt tcgggtgatg cggacttttt cctcgccttc
    11581 ggttacttcg atttcggcga tacccgattc ttcaaccaaa tcaatcagtt tttttaattt
    11641 gcgcaaatcc atttctgttc ctttaaaggc tgccggtgtt ccggcgggtt gttgcgttgg
    11701 tttttcagaa aacagccggc ttgcggcaac ggcgcaaacc gggcggaatg gtgttcggat
    11761 acggttttcc tgtggtggaa aacaaccgtg agaatgtgtt tattttcccg aagttgtcgg
    11821 taaatgtcca gtaaaatatc aaaaaacggc ggtttttggg ggaaatgtgc aggaagcttg
    11881 attttgcgca caagatgccc cgggtcaaga gtgtttattc taatctgttg gtttttcggg
    11941 caaagatgcc gtctgaaaaa gggctaaagt gcgtataatg gcggcttgcc cgaacgagag
    12001 tgtaaaaatg gatatttcag attttgattt taccctgccc gaaaagctga ttgcccagca
    12061 tccgcccgaa gtgcgcggca gcagccgcct tttggttgcg ttgcccgata tgccgctgca
    12121 agaccgggtg ttcggcgatt tgccggatta tgtcgaggcg ggcgacgttt tggtgttcaa
    12181 caacaccaaa gtcatgaagg cgcggctgtt tgggcagaaa gacagcggcg gcaggatcga
    12241 agccctgatt gagcgcgtgt tggacaacca taccgcactg gcgcacatcc gttcgtccaa
    12301 gtcccccaag cccggtatgg ggctggtgtt tgaaggcggc atccgtgccg tgatggtcgg
    12361 gcgtgagggc gaactgttct gcctgcgttt tgaaggcggt caaaccgttt acgaactttt
    12421 ggaacagaac ggccatttgc ctctgccgcc ttatatcgaa cgtgccgccg acgcggacga
    12481 cgacagccgt tatcaaaccg tttatgccaa atatcagggt gcggtcgctg cgccgacggc
    12541 gggcctgcat tttacggaag aacttttgca ccgtctgaaa gacaaaggcg cggtaaccgc
    12601 agaagtaact ctgcacgtcg gtgcggggac attccaaccc gtgcgtgtgg acaaaatcga
    12661 agagcataaa atgcacagcg aatggtttga agtgccgtct gaaaccgccg ccgccgttga
    12721 ggcggcaaaa gcccggggga acaaagtctg ggcggtcggc acgacttcca tgcgcgccct
    12781 cgaatctgcc gcgcgtgcga cgggtcgtct gaaagcgggg cagggcgaca ccgatatttt
    12841 catcacgccg ggctaccgtt ttaatgttgt cgacaggctg gttaccaatt ttcatttgcc
    12901 gaaatcgacg ctgctgatgc ttgtcagcgc gttttcgggt atgggtcata tccgcgccgc
    12961 gtatcgttat gcggtcgaac gcgaataccg tttcttcagc tacggcgatg cgatggtttt
    13021 ggggcggaac gaaggggtag tacgttagaa cagccccaag tctattgggg gaagacaagt
    13081 gccgtctgaa cggacgttcg gacggcattc ttttgttgat ttgaatgaaa ggaactgtat
    13141 gacatagatt caggcgcgag aggcgagaaa ctcagtcatt gttgatgacg cgcaatacgt
    13201 taaaattcat tccgttttgg tcgtataaac atattacata cgccattttt ttgtattcaa
    13261 ggctgcctga aaacagtggg caagaatatc taacccaacg aaaagctgaa aaagcaacaa
    13321 aaaataatct gcaccttagt cggttggtta gtccaacctt aggggtgcag accattcaat
    13381 ctttcaggct gcctttgttt gtttataaga taaatt
//
LOCUS       DAWKOG010000043        13387 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.043, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000043 DAWKOG010000000
VERSION     DAWKOG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13387
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.043"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(110..538)
                     /locus_tag="WJK66_000926"
     CDS             complement(110..538)
                     /locus_tag="WJK66_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family phosphatase"
                     /protein_id="HEZ7578657.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(603..2351)
                     /locus_tag="WJK66_000927"
     CDS             complement(603..2351)
                     /locus_tag="WJK66_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HEZ7578658.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDTYTTLLGLFSHEYFHAWNV
                     KSIKPAAFVPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTLMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKALYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPSAGDFGARFKQNTDHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            2678..4111
                     /gene="ccoN"
                     /locus_tag="WJK66_000928"
     CDS             2678..4111
                     /gene="ccoN"
                     /locus_tag="WJK66_000928"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEZ7578659.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4138..4749
                     /gene="ccoO"
                     /locus_tag="WJK66_000929"
     CDS             4138..4749
                     /gene="ccoO"
                     /locus_tag="WJK66_000929"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEZ7578660.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4754..4924
                     /locus_tag="WJK66_000930"
     CDS             4754..4924
                     /locus_tag="WJK66_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HEZ7578661.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            4948..6045
                     /gene="ccoP"
                     /locus_tag="WJK66_000931"
     CDS             4948..6045
                     /gene="ccoP"
                     /locus_tag="WJK66_000931"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEZ7578662.1"
                     /translation="MNTTSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPEGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSDKDGKAPVKKAEPAPTPAPAAEPAASAPA
                     EAAQAASEAKPAAAEPKAEEKAAPAAKADGK"
     gene            6085..6279
                     /locus_tag="WJK66_000932"
     CDS             6085..6279
                     /locus_tag="WJK66_000932"
                     /inference="COORDINATES: protein motif:HMM:NF024834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ7578663.1"
                     /translation="MPGIPHVGIKADWADRIKKGKDTLHKHAIEGFNTMPAKGGRGDL
                     SDDEVKAAVDYMVNQSGGKF"
     gene            6481..7890
                     /locus_tag="WJK66_000933"
     CDS             6481..7890
                     /locus_tag="WJK66_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ7578664.1"
                     /translation="MDTQIKTEAGQQNNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVSTMPRNQGWYYAMDEIHSDMMLGSTGDYLLETAASL
                     TIIMIISGLYLWWVKRRGIKAMLLPPKGKARSWWRNLHGAFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWILELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVVFCLAV
                     IFIGISGCVMWWKRRPSGAVGMVPPAQKIKLPVWWAMAVPLLLIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            7991..11200
                     /gene="recC"
                     /locus_tag="WJK66_000934"
     CDS             7991..11200
                     /gene="recC"
                     /locus_tag="WJK66_000934"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEZ7578665.1"
                     /translation="MFYLYQSNRLETLAALFARIQKVKPLKSALQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRRLG
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLESLSSDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVGTDDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPLFHALSCLFDLLESRFEVDKVLVLLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVRWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTAMFGRFAAFLETLSDIVRIWRQPATVDEWVGRCRGLLETLFQAEADDQKAVQNLE
                     NEWVKWQAETELAQFSGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMTGTNSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYATALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHADQIAETYIEARREGRDFAQTAARIGAESLLPSG
                     ELGRLWQQNFQTAAKQIDTAVLNSPKLPPLSYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAETRQTFIVQSGETEILAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLAAAEAFAQKQDWEAALKKAQTAYYGNKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLARLLADTLAAAEKREGAGAA"
     gene            complement(11270..12838)
                     /gene="mtrF"
                     /locus_tag="WJK66_000935"
     CDS             complement(11270..12838)
                     /gene="mtrF"
                     /locus_tag="WJK66_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HEZ7578666.1"
                     /translation="MSQTDTQRDGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAAG
                     AYFGLSVPDPRPVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSTII
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTLYPAP"
     gene            13172..13384
                     /locus_tag="WJK66_000936"
     CDS             13172..13384
                     /locus_tag="WJK66_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578667.1"
                     /translation="MKNEIQKIMDKYDPWHEDDFESYEDIAKDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQENVHAMIEEIKNAI"
BASE COUNT         3356 a         3199 c         3555 g         3277 t
ORIGIN      
        1 actgtaaaaa cggagaagga cagaggacgg cagtaaggtt tgccgtcctc tttttcaagt
       61 tgcggtggaa tgccgcttga aggtgtggtt taaacgtttc ggcttgtaat cagacgcggg
      121 cgttgtccag ccgctctctg aagttttcca aatttacgcc tgccgcttgg gcgcggcgga
      181 tgatttcgtc aaccggcata ccttctgccg cccggcggaa gccccacagg agggagcagc
      241 gcgtaccggt ccggcaatag gcaaggacgg gatattcggc ttgtccgatg agttggcgga
      301 aggtttcgac atcgtgtttt tggatgtcgc gtgcggtaac gggttggtga tggaatccag
      361 taacgcctgc ttgttccagc cactgtttga tttgggcgaa gtcgggttgc gattcttctt
      421 cgcggtcggg gcggttgcag atgacggttt tgatgcccag ttgcgcgatt tgttccgcat
      481 cggctttggt cagttgcgga gaaatataga ggtgttcgtc aagtttcaga atggccattt
      541 tgtttccttt actttgcggt tgtttttcag acggcatttt gtgtgcaatg ccgtctgaaa
      601 gtttaaccga acaaccagtt gtccaacagg ttccggtctg tgatatgtag gatggcagta
      661 tccgctgccg ctgcctgaac cgtcaagacg gtttgacgca atatgccggc acggaagaag
      721 tggatattga tttttgcatt gacgtggtat cgggcccatt gtgcggtaaa gtcggtgcag
      781 gcataaccgt ctaaagcaat gattttgtct tgcgggcaca gtgccgcaga ttccgcgctg
      841 ccgccgttga agacatgggt caggacgatg tggtcggtgt tttgtttgaa acgtgcgcca
      901 aaatcgcctg ccgacgggac ggggcagatg tgttctgcgt atccgccgcc gtgttgtcgg
      961 ggaagcggca ggaaggtcag tcccacgcct gcggttgcca ggcattcggc aagcggcaaa
     1021 tcttcggtac tgtataacgc tttttggaaa aaatcttcca aatccaagcc ggtaatttcc
     1081 tgacagcgga tttgccagtg tttttccgga atacccgagt gtgtgtccct ccactcccga
     1141 tagagtttgt ccattaacgt atcgagagaa tgtctgccgt tgcttcggtt gcgtattatc
     1201 agatcaaggc acaatgcggc aagcgcgcct ttctggtagt agctgacgat ggcgttgggg
     1261 ctgttttcat ccggtttgta aaatttgttc cacgcggtaa aactcgattc cgccaaggtc
     1321 tgcctcaaac ggccgcgggt ttgttgtacg cgcgtaatgc cttgtgccag caggtttaaa
     1381 taagattcgg gcgagatggt gcggctgcgt gccaaaaaca aatcgtcgta ataggatgta
     1441 ataccttcga atgcccatag ttgttcggta tagttttctt tgtcgaggtc ataagggacg
     1501 aacgcggcag gtttgatgga tttgacgttc cacgcgtgaa aatattcgtg ggagaaaagt
     1561 ccgagcaatg tggtgtaggt atcgtcggca tcggtcatac cgtacggcgg aaggctgtgg
     1621 cggtcggcga gcagggcggt gctgtcggtg tgttccaaac cgccgtaaat atggtcgccg
     1681 acgtggagca ggaacaaata tttttgaaac ggggcagggg aggaaaacac cgccagttct
     1741 gtttcgcaga tttttttgat atccgaaacc agcctgttgc ggtcgaaatc gggatatatg
     1801 ccgcttaagg caattgtgtg cggaatgcct gccgcctcaa aatctaaaaa ttcaatcaag
     1861 cccatctcga caggtcggtc aatcaattcg gcataagatg ccgcctgaaa gacaaacctc
     1921 cctgtttccg gcagcgttgt ggcaatacgc cattcggacg gaatacccgt caattccaag
     1981 cggtgcggca gcgtttccgt tccttcgact ttcaaaaaca ggcacgatcc gtcaaaaaaa
     2041 ccgcgttctg tcgtcaggaa agaacctcga accgacaaat cgaatgcata tacggtgtag
     2101 cggatttgcc actcgccgcg tacggcggca gcgtgccagc ggttttttgc aatttgttcg
     2161 agcggcatgg acgtgccgtt acaggatgca tggatagaag tgatgtggcg ggaaaaatcc
     2221 cgaatcagat agctgcccgg tacccaattc ggcaggctta tttctatagg aagatcatca
     2281 tcttgtgtga atgtcagcag gatgtgccac tcgtggtcga gaggggacgg ggttatttta
     2341 taatgaatca taactatcaa atatatcgtt aaacgggatg gattaaatac ctttaaaatg
     2401 cagcttaacc tacatcaata aagtgccaca aaaagggaaa attggaaaca aaagcaatgg
     2461 cggtcgggtt gtaatttatt ataacaatat gatttatatg taataaaaaa cagattaagc
     2521 aaagggctcg tcggcggcgc gaatgtaatt ccgcttggcg taataaggca gatatgttaa
     2581 atttagaaaa aattacgctt gcccggattg caccttaaac aggctgcgga agcggcagga
     2641 taaaaatctt tattcccata aattcatgga gactctcatg gacacacaaa cttacaacta
     2701 caaagtggtg cgccagttcg ccatcatgac tgtagtttgg gggattgtgg gtatgttggt
     2761 cggcgttatc gtcgccgccc agctttttgc tcctgccctc gacttgtcta atatcggacc
     2821 ttggttccac ttcggccgcc tgcgtccgct gcacaccaat gcggttattt tcgcatttgg
     2881 cggttgtggc ctgatcggca catcatacta cgttgtccaa cgtacttgta atacccgtct
     2941 tttcggcggt tggctgccgg catttacctt ttggggctgg caggcggtaa tcgttgccgc
     3001 cgtcgtcagc ttccctatgg gttggaccca aggtaaggaa tatgccgaac tggaatggcc
     3061 gatcgatatt ctgattactt tggtttgggt ggcttacgcc atcgtattct tcggtacgat
     3121 tgccaaacgt aagattaaac atatttacgt tgccaactgg ttctacggcg gctttatttt
     3181 ggcggtcgca cttttacaca tcgtcaacaa tatcagcatc ccggccggtt tgatgaagtc
     3241 ataccccgtc tattccggtg cgattgatgc tatggttcaa tggtggtacg ggcataatgc
     3301 cgtgggcttc ttcctgactg ccggcttctt gggtatgatg tactatttcg tacccaaaca
     3361 agcagcccgc cccgtttact cctaccgcct gtccgtcgtt cacttctggg cgttgatttt
     3421 cacctatatg tgggcgggtc cgcaccatct tcactacact gcgctgcctg actggacgca
     3481 atctttgggt atggttctgt ctttgattct gttcgcaccc tcttggggcg gtatgattaa
     3541 cggcatcatg accttgtccg gcgcgtggga caaactgcgt acagacccga ttcttaaatt
     3601 cctgattgta tccttgtcct tctacggtat gtctaccttt gaaggcccga tgatgtcgat
     3661 taaaacggtc aatgcattga gccactatac ggactggacc gtcgcgcacg ttcatgcggg
     3721 tgcgttgggc tgggtaggct ttgtaaccat cggttccgtc tattacatga ttccccgtct
     3781 gttcggcaaa gaacagatgc acagcaccaa gctggtagaa gcacattttt ggattgcgac
     3841 catcggcgtg gttctgtata tcgctgccat gtggattgcc ggtgtgatgc agggtttgat
     3901 gtggagttct ttgaacgatg acggtacgct gacctactcg tttgtcgaat ccgtaaaacg
     3961 caccatgcct tactacgtga tccgttttgc aggtggttta ttgtatctga gtggtatgtg
     4021 cattatggcg tacaacgtgt accgcacagc catcggtggt aaagcagtcg atgccgaaat
     4081 ccctgcggtt tcccaaacac agcaccacta aaatactaag aaagataggc taccaaaatg
     4141 aaattacaac aattggctga agaaaaaatc ggcgttctga ttgtgttcac gctgcttgta
     4201 gtcagtgtcg gtctgttgat tgaagttgtg cccttggcct ttaccaaggc ggcaacacag
     4261 ccggcaccgg gcgtgaagcc ttacaatgcc ttgcaggttg ccggacgcga tatttacatc
     4321 cgtgagggct gttacaactg ccactctcaa atgattcgtc cgttccgtgc ggaaaccgag
     4381 cgttacggtc attactctgt tgccggagag tcggtttacg accatccttt ccaatggggt
     4441 tccaaacgta ccggtcctga tttggcacgt gtaggcggcc gctattccga cgaatggcac
     4501 cgtatccacc tgctgaatcc ccgtgatgtc gtgcctgagt ccaatatgcc ggcattccca
     4561 tggcttgcac gcaataaagt cgatgtcgat gcaaccgttg ccaacatgaa ggctttgcgt
     4621 aaagtaggta ctccttacag tgatgaggaa attgcgaaag cgcctgaggc tttggcaaac
     4681 aaatccgagc tggatgctgt agtcgcctat ctgcaaggat tgggtctggc tttgaaaaac
     4741 gtaaggtaac atcatggata ttaacggtat tcgcgctctt ttcacggtat ggatctttat
     4801 ctgtttcctg ttggtactct atatcgtctt caacaggcgg aataagaaaa actacgataa
     4861 tgccgcaaac agcatttttg atgaaaacca agatgcgcaa gataagaaaa gcgaaaaccg
     4921 ttaatattgt gataacggag caaaacaatg aacacaacat cccaatttac cagtaatttc
     4981 tggaatatat atattgcagt tattgtctta ctgagcttta tcgctttggc ttggctgctg
     5041 ctgtctcaaa atgttgtcaa acgtccgaag aagggcgaag aagtacaaac tacgggtcat
     5101 gagtgggacg gcattgccga atacgacaat ccgctgcccc gctggtggtt ttggctgtgt
     5161 gttttgacgt ggctgttcgg tatcggttat ttggttatgt atccgggtgt cggcgactac
     5221 aaaggtctgc tgaaatggac cagccataac caatatgaaa aagaggtcaa aaaagccgat
     5281 gagcaatacg gcaaactgta tgccaagttt gcggatatgc cgattgaaaa agtggcaaaa
     5341 gaccctcaag ccaagcaaat cgcccaaaac ctgtttaaca cttattgtat ccagtgccac
     5401 ggctctgatg ctaaaggctc taaaggtttt ccgaatctga ccgatagcga ttggttgtgg
     5461 ggcggtgatc ccgataaaat ccacgagacc atcgaaaaag gccgtgttgc gactatgcct
     5521 gcctggggtc ctgctttggg cgaagaaggc gtgaaagacg ttgcccatta tgtgatgtcc
     5581 ctttcaaaac ccgaaggtca gtatgatgag gaacgtgccg cgcgcggaca agccttgttc
     5641 agcggtccgc ctgccaactg tttcacttgc cacggcgata agggacaagg tatccaaggt
     5701 ttgggtccga acctgactga tgacgtgtgg ttgtggggcg gtacgcaaaa atccattatc
     5761 gaaaccatta ccaacggtcg cagcagccaa atgcccgctt ggggacattt cttggataaa
     5821 gacaaactgc atattatgac tgcttatgta tggggtcttt ccgataaaga cggtaaagct
     5881 ccggtgaaaa aagccgagcc tgcaccgact cccgcaccgg cggcagaacc cgcagcctct
     5941 gctcctgcag aagcagcaca agccgcgtcc gaagccaaac ctgccgcagc agaacctaaa
     6001 gccgaggaaa aagctgcacc tgctgccaaa gcggacggca aataggttta tgaaaccgtt
     6061 tgtgccgcct gccatggcaa tgcgattccg ggtattcccc atgtaggcat caaagccgat
     6121 tgggccgacc gcatcaaaaa aggcaaggac acgttgcaca aacacgcgat cgaaggtttc
     6181 aatacgatgc ccgcaaaagg cggtcgcggc gatttgagcg atgatgaggt taaagctgcg
     6241 gttgactata tggtcaacca gtcaggcggc aaattctaac ttgactgagt ttccgatatt
     6301 atcggttatg aaaatccaga ttcagtattg aatctggatt ttttgtttta acgggcatcc
     6361 ggttttaaac gcagactgcc tatttgttgt aatgaatttt gactttagtc ataaagaatt
     6421 agtctttatt gtttataatc gcgcatcatc acgatagccc tattttcaaa aggacgatta
     6481 atggatacac aaatcaaaac tgaagccggt cagcagaata accggcgtta tctgaccgtt
     6541 tggcggtggc atttttatgc cggtctgctg gttatgcctt ttcttaccct gcttgccgtt
     6601 acgggtttgg gtatgctgct gtttgccaat attaccggta aggagggcga gcggattcat
     6661 gttgtgccgc aggcaacggt acaacctctg tctgttcagg cggaagcggc acgcagtgcc
     6721 gttaatccgg aaacttcgtc cgtcgttcag tatattgcgc cgcgtgccga tgatatggtt
     6781 gccgtgttcc gtgtcaacaa tgagggcaag gcaacgatgg tcgcggtcga tccttatacg
     6841 gcaaaagtgg tcagtaccat gccgcgcaat cagggttggt attacgcgat ggatgaaatc
     6901 cacagcgata tgatgctcgg ttcgacaggt gattatcttt tggaaacggc tgcatcgctg
     6961 acgattatca tgataatcag cggtttgtac ctttggtggg tgaaacggcg cggcatcaag
     7021 gcgatgctgc tgccgccaaa aggcaaggcg cgttcttggt ggcggaatct gcacggcgcg
     7081 tttggaactt gggtgtcgtt gattttactg ttgttctgcc tgtcgggtat tgcttgggca
     7141 ggtatttggg gcggcaagtt cgtgcaggct tggagtcagt tcccggcagg caaatggggt
     7201 gtcgaaccga accctgtttc agtcgtgccg acccatggcg aggtattgaa cgacggcaag
     7261 gttaaggaag tgccgtggat tttggagctt acgcctatgc ctgtttcagg gacgactgtg
     7321 ggcaaagacg gcattaaccc cgacgagccg atgacattgg aaaccgtcga ccgttttgcg
     7381 cgggaaatcg gtttcaaagg gcgttatcag ttgaatttgc ccaaaggcga ggacggcgta
     7441 tggactttgt cgcaagactc tatgagttac gatatgatta gtccgtttgc cgaccgcacg
     7501 gtgcatatcg accagtacag cggcaagatt cttgccgaca tccgttttga tgattacaac
     7561 ccgttcggca aatttatggc ggcgagcatt gcgctgcata tggggacttt gggctggtgg
     7621 agcgtgttgg cgaacgtcgt gttctgcctt gccgtcattt ttatcggtat cagcggctgc
     7681 gtgatgtggt ggaaacgccg tccgtccggc gcggtgggca tggttccgcc ggcgcaaaaa
     7741 atcaagctgc ccgtctggtg ggcaatggcg gtgccgctgc tgctgattgc attgcttttc
     7801 ccgaccgcgc tgcttgccat tgccgtgatt tggctgttgg atacgctgct gttgtcgcgg
     7861 attcctgttt tgaggagatg gtttaaatga ccaaatgccg tctgaaaggt tcagacggca
     7921 ttttgtttga aggcggacgg gggaaaggct atataatccc gaatactttt ccgcaacttt
     7981 tgtcgaaatc atgttttatc tgtatcaatc caaccgtctt gaaacgctgg cggcattgtt
     8041 tgcccgcatt caaaaagtca aaccgctgaa atcggcttta cagcccgaac agattattgt
     8101 gcagagtcag gggatgcgcc gctatctcaa tacctgcctc gcccgcgatt tgggcgtggc
     8161 ggcgaatttg tcgttcagcc tgcccgccgg cctgacgtgg aagctgatga aaaaactgat
     8221 tcccggtatt ccggaactca gcccgtttgc acccgaagtc atgcgctggc ggctgctgga
     8281 tttgttccgc agtgaagcat tccggaatac ggcagaattt gaagatgtga ggaatgtgct
     8341 gcaagactat ctgggcagcg gcgaatcggc agattaccag cttgcgggac agcttgcgga
     8401 catattcgac caatacctcg tgtaccgtcc tcagtggata gacgcttggc agcagggcag
     8461 gcggctcggt ttgggcgacg acgaaatctg gcagtccaaa ctgtggcgtt acctcgacga
     8521 tggcaggcag agcgcgccgc accgtgtcgc gttgtgggaa aagctgttgg aatctttaag
     8581 cagtgataag ctgcccgagc gttatttcgt gttcggcatt tccacgatgg cgccgatgta
     8641 tttgcagctt ttgcacaagc tgtccgaaca ttgcgatgtg ttcgtgtttg cactcaatcc
     8701 gagtgggatg tattggggca acgtcatcga agcggcgcaa atcctcaaag gcggcggcga
     8761 tcccgattta actcaggcag ggcatccgct gctcgcctca ttgggcaagc aggggcgcga
     8821 ctttttcgac tttttgaacg aaatggaaat agaaggagaa acgccggtat ttgaagaagg
     8881 cgggcgcgat acgcttttgc acgccctgca aaccgatatt caaaacctga aaatgccgtc
     8941 tgaaaatgtg ggaagcgtcg gtacggatga cggttcgata cgtatcgtct ccgcgcacag
     9001 ccctttgcgc gaattgcaga tactcaaaga caagctgttg aaaattctgc atgaacatcc
     9061 cgattggcag ccgcatgata tcgccgtatt aaccccgaat atcgaatcct atacgccttt
     9121 tatcgaggcc gtgttcggac aggcgcagcc cggtgcgcag gcattgccgt attccgtttc
     9181 ggacgtgaaa ataagccgcc gacaaccgct gtttcatgct ttgtcatgcc tgttcgactt
     9241 gttggaaagc cgatttgaag tcgataaagt gcttgtgctt ttagaaaccg cacccgtgtt
     9301 gcgccgtttc ggactgactg aggacgattt gccgcttttg cacgacatgg ttgccgattt
     9361 gaacgtccgc tggggtttgg acggagaaat gcgcggcggc acggatcagc tgtttacctg
     9421 gaagcaggcg gtagaacgca tgatattggg ctggatgctg cccgaaggcg gcaatccgat
     9481 gtggcaggat gtcagcgcgt ggtatgccga cgtgaatcaa accgccatgt tcggacgttt
     9541 tgccgccttc ctcgaaaccc tgtcggatat tgtacggata tggcggcaac ctgcgacggt
     9601 tgatgaatgg gtaggacgtt gccgaggttt gcttgaaaca ttgttccaag ccgaagccga
     9661 tgaccaaaag gcagtccaaa acctcgaaaa cgaatgggtc aaatggcagg cggaaacaga
     9721 attggcgcaa ttttccggac agttgccgca acaaaccgtc atccgccata tccgacgttt
     9781 cctcgacagc gaaagcgagg caggcttttt acgcggcggc atcacctttt gcagtatggt
     9841 gccgatgcgg agcctgccgt tcaaagtcat ctgcctgttg ggtttgaacg acggagattt
     9901 tccccgcaat accaaagccg ccgtattcga cctgattgcc aaacatcccg ccaaaggcga
     9961 ccgagcccgt cgcgatgacg accgctacct gttccttgaa gctctcatca gcgcgcgtga
    10021 aatcctgtat ttgtcctaca tcgggcgcga tatccgcaaa gacgaagagc ttgccccgtc
    10081 ttcgctgttg ggcgaactca tcgacacagt tgccgccatg accggcacaa acagccgcca
    10141 acttgcacaa aattggatag aacagcatcc gctgcaagcc ttctcgcgcc gatatttcca
    10201 agaaggcgga cgttcagacg gcatattcgg cacgcgtacc gactacgcca ctgcgctcgg
    10261 acaaacgccc gaaccgccgc aacccttttt cgaccaaccc gtagaaaacg ccgaacctgt
    10321 tgccgaaatc ggacaggacg aatttatccg tttctggcgc aaccccgtca aagtatggct
    10381 tcagcagcag cttgcgtgga gcgaacccca tatcggcgaa gcctgggagc ctgccgaacc
    10441 cttcgagccg caacacgccg atcaaatcgc cgaaacctac atcgaagcac ggcgcgaagg
    10501 acgggatttt gcccaaaccg ccgcccgcat cggggcggaa agcctcctgc cgtcgggaga
    10561 gttgggcaga ctttggcagc agaacttcca aactgctgcc aaacaaatcg acacggcggt
    10621 tttaaacagc cccaaactgc caccgctttc atatgccata ccgtcggacg ggcaaatcct
    10681 gaaaggcagc ttgggcaatc tgtaccgctg cggacaagtg ttttacgcct acggcaaacc
    10741 caacgcgccg caacgtatcg cttttctgct ggaacacctg atattttgcg ccgttatgcc
    10801 gtctgaagcc gaaacgcggc aaacctttat cgtccaatcc ggagaaaccg aaatattggc
    10861 ggaaatcgcg caagacaggg cattgcagct attgtcggaa tggatggcgt ttttcaatat
    10921 cgggcaaaac cgcccgctgc cgttttttgc caagacctcg cttgccgctg ccgaagcgtt
    10981 tgcccaaaaa caagattggg aagccgccct gaaaaaagcc caaaccgcct attacggcaa
    11041 caaagtcagc aaagggcaga aagactatac cgaagtcgcc ctcgtgttcg gcaacgcaag
    11101 ccaaaaccca ctcgaacaac ccctgtttga aaacctcgcc cgcctgcttg ccgacacgct
    11161 tgccgcagcg gaaaaaaggg aaggggccgg agcagcctga cgggatggaa aggcaaatgc
    11221 cgtctgaaaa cagcaaaaag gttttcagac ggcattttgt ttatcgtgtt taaggtgcgg
    11281 gatacaaagt gggtgcgccg ggaccgacgg gcaggcccaa aacaaatacc caaatgcaga
    11341 ataaggcaat ccatgcaatt aagaagaaag cggaatacgg caacatcata gaaatcagcg
    11401 tacccacgcc cgcatctttt ttgtatttga tcactgtcgc cataatcagc ccgaaataac
    11461 tcatcatcgg cgtaataata ttggtaacgg aatcaccgat gcggtaagcg gcttgaatga
    11521 cttcgggcgc gtagccggcc aacatcagca tagggacgaa aatcggcgca gttaccgccc
    11581 attgcgcgga ggcggagcct atcatcagat tgataaaagc acaaattaaa ataaaaccga
    11641 taaacaacac gctgccgccc aagccgactt cttttaagaa cgtcgcccct ttaacggcaa
    11701 tatattgccc aatattcgtc caattaaaaa atgcgacaaa ttgtgcggca aaaaagatga
    11761 tgaccaaata aagccccaga gtactcatcg attcggccat cgcattaacg acttcctgtt
    11821 cgccgcgcaa acttcgggtt acccggccat aaacaatgcc cggcagcgca aacaacaaga
    11881 aaataaaaac aacaatcgat tttaaaaacg gcgaaccgga aaccaatcct gtttcaggat
    11941 gacgcaaaat accgtcggca gggacgatgc tccaagccaa tagggcggat aaggcaacaa
    12001 acaccacgcc agcccaaatt aatcctttat attccaaagg cgtgatttca ttggaatgcc
    12061 gaatgtcttt ttcttcttgt gacaaatctg attgataagg gcccaattgc ggttcgacga
    12121 ttttttcagt aacaaaataa ccaatcaaag caatcacaaa cgtactggct accataaaaa
    12181 accagttggc ttcagggcct acgacgtagt cgggatggat gatttgcgcc gcctgttggg
    12241 tgatgcctgc caagagcgga tcgattgtgc ctaagaacag attggccgaa taaccgcccg
    12301 aaacgccggc gaaagccgca gccagaccgg caagcggatg gcggccgagg gaatgaaaga
    12361 tgatggtgga caaagggatt aggacgacat agcccaattc agaagcggta ttagataaaa
    12421 tccctgtaaa aacaaccata aaagtagtga gtttgcgtgg agattttgtg agcaataagc
    12481 gcattaatgc ggaaatcaag cccgattttt ccgcaatccc cacgcccaat aaagaaacca
    12541 acaccgttcc caacggcgcg aaaccggtga aatttttaac ggtatgcgtc aggattttga
    12601 tcaaaccgtc agcatcgagc aggctgacaa cgtgaatcaa accgtcatcg gcacgtcctt
    12661 tcgcaccaac agggcgcgga tcggggacgg atagtccgaa atacgcaccg gcggcagagg
    12721 caatcagcaa taacacaatg aaaataataa aaagcgtaac cgggtgcggc aacatattgc
    12781 ccagccattc gactgtgcgt aaaaatcgtc cgtcccgttg cgtatcggtt tgactcatct
    12841 gcttctcctt aaaaaaaata tattgagtgc attaaatttt tgaatgtaac atgaagtgtt
    12901 ttgtaagcaa actaaaataa tcgtatatgt atataaccgt aatagattaa aacagaaaat
    12961 gcccacatcg ttattctcat aaaggcggta atccgaaaac ttgacgttaa agccttatca
    13021 ggaatgactg gaactcaaca aagcggtttc ccgctttcgc ggaaatggcg ggattttcgg
    13081 tttttgtttg caaaaatgcc ggagaaaaat gcagtttaca ttttgaatgg tattgttatt
    13141 aattgtaaat aaatattgga aggtaaatga tatgaaaaat gaaattcaaa aaattatgga
    13201 caaatatgat ccatggcatg aagatgattt tgagtcgtat gaagacattg ccaaagatgt
    13261 atcgttgatg acagataaaa cgttcattga acattatttg ttagaagttt attcagaaga
    13321 aaacggacat tttgaccaag agaatgtcca tgcaatgata gaagaaatta aaaatgcaat
    13381 ttagtga
//
LOCUS       DAWKOG010000044        13254 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.044, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000044 DAWKOG010000000
VERSION     DAWKOG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13254
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.044"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            172..2318
                     /locus_tag="WJK66_000937"
                     /pseudo
     CDS             172..2318
                     /locus_tag="WJK66_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            2308..5088
                     /locus_tag="WJK66_000938"
     CDS             2308..5088
                     /locus_tag="WJK66_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEZ7578668.1"
                     /translation="MANNKTLFDWVEDRKSTLEEIEQTDFFALPEFVERNLKYPFFEW
                     QKSALENFVIFDRASKLKDFPDIKNRPTHLLFNMATGAGKTMMMAALILYYFEKGYRH
                     FLFFVNQNNIVDKTENNFIDPAHAKFLFTEKILQGDTVIPIRKVEMFSPHSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHKLNLVMLGDEAHHLNAQTKGKKQDELDLEVELKA
                     NAGNAEIERKGWEHMVLELLLNKNGNPGQNVLLEFTATLPENADVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSSTLNKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     IDESKADYSAFLNWAENVQADDFEFLDTFSGSLNNSDNANEQGKTRTEQALKFMQENG
                     FEFAHLANWVKQNYQKHNVIITNSETNKNKTEKTDSETEKLLNNLEAADNPIRAIFTV
                     DRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAFEG
                     KQPNKRKFDNDMQHELRILEELFYYTHDEQSRYISELKNELRKDGYLPEKDDDKVLAT
                     FKLKSEFADNKDFRELLIWANKKIPNPNAKSNNADSLQANPQTLSFQVHGNQLLQETQ
                     FTADENDETARQIGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFNRLQSKLD
                     IQNRNELQNNLLKDWQIEFLGLGQDKQVRLDDKLAGCLKILEMVEKHLNESDMPFIGT
                     KEFTPKKLWEIFGTPKQKWVKKDDVKTTIATQNDWYVMNNFAGTGLEEALIQFISARL
                     GDLKSKYDVHLIRNEEVFKLNNFADGEGFMPDFILLLKDKQKSSSNGVKDFLHYQIFI
                     EPKGEHLVETDGWKEEFLEAITVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFTES
                     FPLGAASLEKMK"
     gene            complement(5232..6404)
                     /locus_tag="WJK66_000939"
     CDS             complement(5232..6404)
                     /locus_tag="WJK66_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEZ7578669.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALV
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            6694..7140
                     /gene="iscR"
                     /locus_tag="WJK66_000940"
     CDS             6694..7140
                     /gene="iscR"
                     /locus_tag="WJK66_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEZ7578670.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            7169..8383
                     /locus_tag="WJK66_000941"
     CDS             7169..8383
                     /locus_tag="WJK66_000941"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEZ7578671.1"
                     /translation="MTVKTPVYLDYAATTTVDKRVAEKMIPYLTETFGNPASNSHAFG
                     WEAEEAVEKARADIAALINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILVSVMWANNEIGVV
                     QDIPAIGEICRERKIVFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDI
                     FLKGIEGIEEVYINGDLEHRVPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            8644..9030
                     /gene="iscU"
                     /locus_tag="WJK66_000942"
     CDS             8644..9030
                     /gene="iscU"
                     /locus_tag="WJK66_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEZ7578672.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            9117..9437
                     /gene="iscA"
                     /locus_tag="WJK66_000943"
     CDS             9117..9437
                     /gene="iscA"
                     /locus_tag="WJK66_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEZ7578673.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(9397..9618)
                     /locus_tag="WJK66_000944"
     CDS             complement(9397..9618)
                     /locus_tag="WJK66_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HEZ7578674.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRRE
                     AKKWRDGFDTVPPFLCLKHGSFRRSRRSL"
     gene            9700..10200
                     /gene="hscB"
                     /locus_tag="WJK66_000945"
     CDS             9700..10200
                     /gene="hscB"
                     /locus_tag="WJK66_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEZ7578675.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            10298..13048
                     /gene="gyrA"
                     /locus_tag="WJK66_000946"
     CDS             10298..13048
                     /gene="gyrA"
                     /locus_tag="WJK66_000946"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEZ7578676.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVMRGKTHIEPIGRNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKDKLLARPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
BASE COUNT         4049 a         3257 c         2910 g         3038 t
ORIGIN      
        1 ttccgcaaaa tcacgacaca ctcggacacc cgccccgctt atcgcaactt tgcgaacgcc
       61 cccggaaaca gcaaagacat caaataattg attttattag aatctatttg caaagccatt
      121 tgccgttaca caagaatggc acattaaaat aactgatgag gatttataac gatgaagaca
      181 gacattcaaa ccgaattaac ccaagcccta ctaccacacg aaaaagtatg ggcgaacgaa
      241 gaaaaaacca ttttagccaa aaacatcctg ttggatttgg tggaaaaaac cgacccgacc
      301 attatcggtt tgttattgag taatgatgag ttaaaacgcc atttctttgt ggaagtgaat
      361 ggtgtgctgg tgtttaaatt gcaggatttc cgttttttct tggacaaaca cagcgtcaat
      421 aattcctaca caaaatacgc caaccgcatt ggtttgacgg acagcaaccg ctttttgaaa
      481 gacagcagtg atattgtgtt ggattttccg tttaaagatt gtgtgttaaa tggcggacaa
      541 agcaccgagg aaggcgaaga aatttatttt aaacgcaata acagccagcc agccaattat
      601 acaccaaatt aacccgaaaa agacaagaaa tcttttttaa tcaaacgctt gcttttgatg
      661 aaattgaccg actttttgac gcaaaagcat tctcaaaatt ctctcgctat accgcagacg
      721 gcaaacaagc ggttggcgaa atcaaacgcc attcagacgg cacaccggcc gaaaatctta
      781 ttatcaaagg caataatctg attgccctgc attcgcttgc caagcagttt aaaggcaaag
      841 tgaagctgat ttatattgat ccgccttatt attttgttaa aaagaaacaa caagatagtt
      901 ttggttataa cactaacttt aaattatcta cttggcttac atttatgaaa aatcgtttat
      961 caattgccaa agaattatta actgatgatg ggattattgt tatttccatt gatgatgacg
     1021 gaaatgctta tttaaagatt ttgttagatg aaatttttgg atttgaaaat tttatcggaa
     1081 atttacccac tattatgaat ttaaaaggta ataatgatga atatgctttt gcaggcactc
     1141 acgaatacac actagtcttt gctaaaaata aagataaatc tactttttat gaatttccaa
     1201 tagatgaaga taatttttta gagaaatggg aagaagatga aattgggttt tataaaaaag
     1261 gagctccaat gcgagcaaca ggaaccgaag ataaaagaga agatagacct gaaatgttct
     1321 atcctttttt ggtaaaaaat aacacagtta gtacaattac agatgaagaa ttttctcaaa
     1381 tctataataa agatttagaa gtattcaatg atgattttat tcaaaaacta aaagagaaat
     1441 acgaaaatct tggatacaat tttattttac ctattgctaa taaacaatgg gggcgttggc
     1501 gttgggggta ttcaataaag aataaggcaa ggctgcagac ggatattatt gttagccaat
     1561 ctaaaaatgg aatttccttg tataaaaaac aaagacctga attagatgat ttacctacaa
     1621 aaaaacctaa aactattttt tataaacctg aatacagtag tggtaatgga actgaacaga
     1681 tgaaaaatct ttttggtgaa aaggcattta aaaatcctaa accagaagaa cttatccaag
     1741 attttattac catcacaacc aacgaaaacg acatcgtcct agactaccat ttaggcagtg
     1801 gcacaaccgc cgccgttgcc cacaaaatga accgccaata tatcggtatt gaacaaatgg
     1861 attatattga aacgcttgcc gttgaacgct tgaaaaaagt gattgatggc gagcaaggcg
     1921 gtatttccaa agccgtgaat tggcaaggtg gtggcgagtt tgtttatgcc gaacttgccc
     1981 catttaacga aaccgcaaaa caacaaattt tggcttgcga agattcagac ggcatcaaaa
     2041 cgctgtttga aggtttatgc gaacgctatt tcttgaaata caacgtcagc gtaaatgaat
     2101 ttagtcaaat cattcaagag cctgaatttc aatccttggc attagacgaa caaaaacaaa
     2161 tggtgcttga aatgttggat ttaaatcaaa tgtatgtttc attatccgaa atggatgatg
     2221 agcaatttgc aggttgctta aatgatgacg ataaagcgtt aagccgtgaa ttctatcaat
     2281 cagcaaaaaa tcaagcggag aaaaaagatg gcgaataata aaacgttatt tgattgggtg
     2341 gaagaccgca aatcaacgct ggaagaaata gaacagacag attttttcgc gctgcctgaa
     2401 tttgtagaac gcaatttaaa atatccgttt tttgaatggc aaaaatcggc tttagaaaac
     2461 tttgtgattt ttgaccgcgc ttcaaaatta aaggatttcc ctgacatcaa aaaccgccct
     2521 acccatttgc tgttcaatat ggcaacaggt gctggcaaaa cgatgatgat ggcggcgttg
     2581 attttgtatt attttgaaaa aggttatcgg cattttttgt ttttcgtcaa tcaaaataat
     2641 atcgtggata aaacggaaaa taattttatt gacccggcgc acgcaaaatt tttatttacc
     2701 gagaagattt tgcaaggtga tacggtaatt cctattcgca aagtggaaat gttcagccca
     2761 cattcagacg gcattgaaat taaatttacc agcattcaaa aactgtataa cgatatccac
     2821 accgagcgag aaaaccaaac cacattggca gatttgcaca aattgaacct tgtgatgctg
     2881 ggcgatgaag cacaccattt aaacgcacaa accaaaggca aaaaacaaga cgagttggat
     2941 ttggaagtgg aattaaaagc caatgcaggc aatgccgaaa ttgaacgcaa aggctgggag
     3001 catatggttt tggaattgtt gctcaataaa aatggcaatc ccggccaaaa tgtgctgttg
     3061 gaatttaccg ccacgctgcc tgaaaatgct gacgtgcaac aaaaatatgc ggacaaaatc
     3121 atcacaaaat ttggcttaaa agagtttttg caaaaaggtt ataccaaaga aatcaatttg
     3181 gtatccagta cgctgaataa gaaagagcgg gtgttgcacg ctttattatt tgcttggtat
     3241 cgccatcaaa tcgcgttgaa atacggcatt gccaatttca agcctgtgat gttgtttaga
     3301 agtaagacga ttgatgaatc aaaagcggat tattcggcat ttttaaattg ggcagaaaat
     3361 gtacaagcgg atgattttga gtttttagat acgttttcag gcagcctgaa taatagcgac
     3421 aacgccaacg aacaaggcaa aacccgaacc gaacaagccc taaaatttat gcaggaaaat
     3481 ggctttgagt ttgcacattt ggcaaattgg gtgaaacaaa attatcaaaa acataatgtg
     3541 attattacca actccgaaac caacaaaaac aaaactgaaa aaaccgacag cgaaaccgaa
     3601 aaattgctaa acaatttgga agcggctgat aatccaatcc gtgccatttt tacggtagat
     3661 agattaaccg aaggttggga cgttctgaat ttgtttgata ttgtgcgttt gtatgaaggg
     3721 caaaatggcg gtggttcaaa taaaaaatca ggcaaaacgg ctgccgctac cgtatcggaa
     3781 aagcagttga ttggtcgtgg cgtgcgttat tttccatttg cgtttgaagg taaacagccg
     3841 aataaacgca aatttgacaa cgacatgcaa cacgaattgc gtatcttgga agaattgttt
     3901 tattacacgc acgatgagca atcccgctat atttcagaac tgaaaaatga gttgcgaaaa
     3961 gacggttatt tgcctgaaaa agacgatgat aaggtgttgg caacatttaa actcaaatct
     4021 gaatttgccg ataataagga ttttagagag ttgttaattt gggcgaataa aaaaatcccc
     4081 aacccaaacg ccaaaagcaa taatgcagac agcctgcaag ccaatccgca aacgctttca
     4141 ttccaagttc acggtaatca actgttgcag gaaacgcaat ttacagccga tgaaaatgat
     4201 gaaacagccc gacaaatcgg cacacaaaat aattttactc aaaccataaa aatgagtgaa
     4261 atggagcggc acattttcaa taaagcatta catatcaaag ggaaaaatag tcaatcttta
     4321 ttccatttca accgtttgca aagcaagctc gacattcaaa atcgcaatga attgcaaaat
     4381 aacttgttga aagactggca aattgaattt ttaggattag ggcaagacaa acaggttcgc
     4441 ttggacgata aacttgcagg ctgcctgaaa atcttggaaa tggttgaaaa acatctgaat
     4501 gaaagtgata tgccgtttat cggtacaaaa gaatttacac ctaaaaaatt atgggaaatt
     4561 ttcggcacgc caaaacaaaa atgggtcaaa aaagatgatg taaaaacgac tattgccacg
     4621 caaaatgatt ggtatgtgat gaataatttt gccggaacgg gtttggaaga agcgttaatt
     4681 caatttattt cagcgcgttt gggcgatttg aaatctaaat atgacgttca tttaatccgt
     4741 aatgaagaag tgtttaaatt gaataacttt gccgatggtg aaggatttat gccggacttt
     4801 attttattgc tgaaagataa acaaaaatct tcttccaatg gcgtgaagga ctttttgcat
     4861 taccaaattt tcattgagcc caaaggtgag catttagtgg aaactgatgg atggaaagaa
     4921 gaatttttag aagccatcac tgtggaatat gggatggata agatcctgca aaaagataca
     4981 ccgcattatc gtttgatcgg tttaccgttt tttactgatc atcagaaaaa tggacaattt
     5041 acggagtcat tccctttagg tgcggcatcg cttgaaaaaa tgaagtagtg cattgcaggc
     5101 aaccccgttt gacaaaactt cctttacaaa agggcgtttt gtcagatatt taatcaacac
     5161 attattaaaa tacagccaaa ttttaatgcc gtccgaaccc tgtgttcaga cggcatcgta
     5221 tttttcagta tctaaaccgt ttccctgccc caatctttgc ctctcaaaat cgaagcatcg
     5281 acatcttgaa tatcgcggtg tcccgtaaac gccatagata tatccatttc tttatacagg
     5341 atttccagcg cacgggttac gccttcttca ccatacgcac ccaaaccata cagaaacgcc
     5401 cgacctatca ttgtaccttt cgcacccaaa gcccacgcct tcaaaatatc ctgaccgctg
     5461 cggatgccgc tgtccatcca aacttcgatg tcgctgccga ctgcactaac gatgtcgggc
     5521 aaggctttga tggcggacac ggtatcgtcg agctggcggc cgccgtggtt ggaaacgacc
     5581 aatgcgtccg caccgctttt cgctgctttt tccgcatctt caggttccat aatgcctttg
     5641 ataatcagct tgccacccca caaatcttta atgcgtgcaa cgtcgtccca gctcaggcgc
     5701 gggtcgaatt gttcggaagt ccatgaagac agcgaggaca aatcgccgac gtttttggcg
     5761 tgtccaacga tattgcggaa cgtgcggcgt tccgtgttca gcattttcat acaccattcg
     5821 ggcttggtcg ccagattgat taaattggcg atggtcggtt tcggcggcgc ggacaggccg
     5881 tttttgatgt ctttgtggcg ttgccccaaa acctgcaaat cagcggtcaa taccaatgcc
     5941 gaacatttgg catccttcgc gcgcttaatc aggttttcca taaactcgcg gtcgcgcatc
     6001 acataaagct gaaaccaaaa tggtgcggaa gtgttctcgg caacgtcttc aatcgagcag
     6061 atagacatcg tggacagcgt aaacggaatg ccgaacttct ccgccgcccg cgccgccaaa
     6121 atttcaccgt cggcgtgtgc catacccgtg aaacccgtcg gcgcaatcgc caccggcatt
     6181 ttcacatcct gcccgatcat tttggtctcc aggcttcggc cttccatatt gaccaatacc
     6241 ttttgtcgga agcggatgtc tttgaaatcc gaagtgtttt cacggtaggt agtttctgtc
     6301 cacgaacccg aatcgatgta atcgtaaaac atacgcggca ttttgcgctt ggcaacgcgg
     6361 cgcaagtctt cgatgcaggt cattttgctc aaatcacgtt tcatttgtcg ccccctgaat
     6421 acctgaataa ctttatatga aatagataat gtatatcaat attgattata aggcaaatca
     6481 tttcaacatt tactgcaccc gccgccgctc ccacttcaag cggcataagg tttagaattc
     6541 aaaaataata tattaaaatc aaaatattaa aaatcaatca atctgccgat ttaaacagcc
     6601 aatcgcgcaa tccgccccca tacttgactg aaacactcag atattggaca attccgccca
     6661 ctataaaaaa agccgacacg ggcaaccacc accatgagac tgaccaccaa agggcgtttt
     6721 gccgttaccg ctatgctgga tttggcgatg aacgcgcaaa ccggcgccgt caaactcagt
     6781 gccatcagcg aacgccaaaa catatccctc tcctatctcg agcaattgtt cggcaaactc
     6841 cgccgcgccg gacttgttga aagcctgcgc gggcccggcg gcggctacat cctcgccgca
     6901 ccggcagcac gcatcaacat cgcccaaatc atcgccgccg ccgaagaccg gctggacgca
     6961 acccaatgcg gcagcaaagc caactgccac cacggcgcgc cctgcctgac gcacgacctt
     7021 tgggagaatt taaacaaaac catcaacgac tacctcggca gcgttaccct gcaaagcatc
     7081 atcgaacaga aaaacaacgg cgacggcagc cgcgtcgtcc aatttacaca catccattaa
     7141 ataacacccg aaaaagaaag agcaaaccat gaccgtcaaa acccccgttt acctcgacta
     7201 cgccgccacc accaccgttg acaaacgcgt tgccgaaaaa atgattccct atctgaccga
     7261 aaccttcggc aaccccgcct ccaacagcca cgcattcggc tgggaagcag aagaggccgt
     7321 cgaaaaagcc cgcgccgaca tcgctgccct gattaatgcc gaccccaaag aaatcgtctt
     7381 caccagcggc gcgaccgagt ccgacaacct cgccatcaaa ggcgcggcaa acttctacaa
     7441 aaccaaaggc aaacacctca ttaccgtcaa aaccgaacat aaagccgttt tagatacaat
     7501 gcgcgaactc gaacgccaag gttttgaagt aacctacctc ggcgttcaag aaaacggttt
     7561 gattgatttg gaagaactca aagccgccat ccgcgacgac accatcctcg tttccgtaat
     7621 gtgggcaaac aacgaaatcg gcgtggtgca agacattccc gccatcggcg aaatctgccg
     7681 cgaacgcaaa atcgtcttcc acgtcgatgc cgcacaagcc tgcggcaaag tgcctgtcga
     7741 tgtcgaagcc gccaaaatcg acctgctctc gatgtccgcg cacaaagtgt acggccccaa
     7801 aggcatcggc gcgctgtacg tccgccgcaa accccgcgtc cgcctcgaag cccaaatgca
     7861 cggcggcggc cacgaacgcg gcttccgcag cggcacattg ccgacccacc aaatcgtcgg
     7921 tatgggcgaa gccttccgca tcgccaaaga agaactcgaa caagatatgg cgcactaccg
     7981 caaactgcgc gatattttcc tcaaaggtat cgaaggcatc gaagaagtct atatcaacgg
     8041 cgacctcgaa catcgcgtcc cgaacaacct aaacgtcagc ttcaacttcg tcgaaggcga
     8101 aagcctgatt atggcagtga aagaactcgc cgtatccagc ggctccgcct gcacctccgc
     8161 ctcgctcgaa cccagctacg tcctgcgcgc attgggcaga aatgacgaat tggcgcactc
     8221 gtccctgcgc atcaccttcg gtcgcatgac cactgaagaa gaagtgcaat ttgccgccga
     8281 actgattaaa tccaaaatcg gcaaactgcg cgaactgtcg ccgttgtggg aaatgttcaa
     8341 agacgggatt gatttgaact cgattgaatg ggcggcgcat taaagcgtac caacatgcca
     8401 tccgaacctt cagacggcat tccaaaaaca aagcaatcaa gagaaaaata tgaacgaaca
     8461 agatttagat ttggacaacc tcgacaacct gcttgacgat ttcgacggcg ttaccgtgga
     8521 aggcggcgtc gattcggaaa acgacgacgg ctgtgaaggc ggagcgtgca aaatctaact
     8581 caggccgtct gaaaaccggc aaacaaacca cttaaatcat caaaacatta aggaaaccac
     8641 atcatggcat acagcgataa agtaatcgac cactacgaaa atccccgcaa cgtcggtact
     8701 ttcgacaaaa acgacgaatc tgtcggcacc ggcatggtcg gcgcaccggc ctgcggcgac
     8761 gtgatgcgcc tgcaaatcaa agtgaacgat gaaggcatca tcgaagatgc gaaattcaaa
     8821 acttacggct gcggttccgc catcgcttcg tccagcctga ttaccgagtg ggttaaaggc
     8881 aaaagcctgg atgacgcgct ggcaatcaaa aacagcgaaa tcgccgagga gttggaattg
     8941 ccgccggtaa aaatccactg ctccatcttg gctgaagatg cggtaaaagc ggccgttgcc
     9001 gactaccgca aacgtcagga aaacagataa agcccttcag acggcatcgt cccgcaatgc
     9061 cgtccgaacc acccgccgct tcaggtccgt ccggggcggt acaacaagga agaaatatga
     9121 ttacccttac cgagaatgcc gcaaaacaca tcaatgacta tctcgccaaa cgcggcaaag
     9181 gcttgggcgt acgcttgggt gtgaaaacca gcggctgctc ggggatggcg tacaaccttg
     9241 aatttgtcga cgaagccgat ggcgacgacc tgattttcga aggacacggc gcgcgcattt
     9301 atatcgatcc gaaaagcctg gtttatctgg atggcacgca agtcgattac accaaagaag
     9361 gtttgcagga aggtttcaaa tttgaaaacc ccaatgtcaa agactcctgc ggctgcggcg
     9421 aaagcttcca tgtttaagac ataaaaatgg cgggaccgta tcaaaaccgt cccgccattt
     9481 tttcgcttcc cgcctgttgt agctgccttt gccttttctt ttccgttcca ccttgtgtcg
     9541 gaacaaatca gatttcacta aggcttttaa agcattgtcg cgtattttgc ctttattgtg
     9601 ctgcactttg ccgcccatat tcagtccttt cgtttaagaa gcggcagatt ataaggcaaa
     9661 aacagttttc tgccaaaatc ttacatttat catcctacta tgtcccaata tttcaccctc
     9721 ttccggattg aacccgcttt cgatatcgac accgaaaact tggaacaaac ctaccgcgcc
     9781 ttggccgccc gtttccatcc cgataaattc gcttcagctt ccgcctttga gcaaaagcag
     9841 gcagtgatga tgtcttccac catcaacgat gcctaccgca ccttgaaaaa ccccatcgac
     9901 cgcgccgcct acctgctgaa aacatcgggc atcgatgccg acgcgccgga gcataccgct
     9961 ttcgcccccg aattccttat gcagcaaatg gaatggcgcg aaacgctgat ggaggcacgg
    10021 gcaggcaacg accttgaatc cttgaaaaat ctcgacaacg aaatccgcga cgaacaagaa
    10081 aaactgttct gcggtctgaa acagtcgttt gcccgacaag attacgacac agccgcacaa
    10141 caagtccgcc aaggcaggtt tctcgacaaa ctccgcaacg aaatttcctc ggcattataa
    10201 tccgcaccgt gtttcagacg gcgtaaccgc cgcaccgttc cgcgtcaaaa tatgctaaaa
    10261 taagcaacaa ttttttgcca tacgaaacat tgaaaccatg accgacgcaa ccatccgcca
    10321 cgaccacaaa ttcgccctcg aaaccctgcc ggtaagcctt gaagacgaaa tgcgcaaaag
    10381 ctatctcgac tacgccatga gcgtcattgt cgggcgcgcg ctgccggacg ttcgcgacgg
    10441 tctcaagccg gtacaccgcc gcgtactgta cgcgatgcac gagctgaaaa acaactggaa
    10501 tgccgcctac aaaaaatcgg cgcgcattgt cggcgacgtc atcggtaaat accaccccca
    10561 cggcgatacc gccgtatacg acaccatcgt ccgtatggcg caaaatttcg ctatgcgtta
    10621 tgtgctgata gacggacagg gcaacttcgg atcggtggac gggcttgccg ccgcagccat
    10681 gcgctacacc gaaatccgca tggcgaaaat ttcccacgaa atgctggcag acattgagga
    10741 agaaaccgtc aatttcggcc cgaactacga cggtagcgaa cacgagccgc ttgtactgcc
    10801 gacccgtttc cccacactgc tcgtcaacgg ctcgtccggc atcgccgtcg gcatggcgac
    10861 caatatcccg ccgcacaacc tttctgatac cgtcaatgcc tgcctgcgcc tgctcgatgc
    10921 acccgacacc gaaatcgacg aactgatcga cattatccaa gcccccgact tcccgaccgg
    10981 ggcaaccatc tacggcttga gcggcgtgcg cgaaggctat aaaacaggcc gcggccgcgt
    11041 cgttatgcgc ggtaagaccc atatcgaacc cataggcaga aacggcgaac gcgaagccat
    11101 cgttatcgac gaaatcccct atcaggtcaa caaagccaag ctggtcgaga aaatcggcga
    11161 tttggttcga gaaaaaacac tggaaggcat ttccgagctc cgcgacgaat ccgacaaatc
    11221 cggtatgcgc gtcgttatcg agctgaaacg caacgaaaat gccgaagtcg tcttaaacca
    11281 actctacaaa ctgactccgc tgcaagacag tttcggcatc aatatggtgg ttttggtcga
    11341 cggacaaccg cgcctgttga acctgaaaca gattctctcc gaattcctgc gccaccgccg
    11401 cgaagtcgtt acccgacgta cgcttttccg gctgaagaag gcacgccatg aagggcatat
    11461 cgccgaaggc aaagcggtcg cactgtccaa tatcgatgaa atcatcaagc tcatcaaaga
    11521 atcgcccaac gcagccgagg ccaaagacaa actgcttgcg cgtccttggc gcagcagcct
    11581 cgttgaagaa atgctgacgc gttccggtct ggatttggaa atgatgcgtc cggaaggatt
    11641 ggctgcaaac atcggcttga aagagcaagg ttattacctg agcgagattc aggcagatgc
    11701 tattttacgc atgagcctgc gaaacctgac cggcctcgat caagaagaaa ttgtcgaaag
    11761 ctacaaaaac ctgatgggta aaatcatcga ctttgtggat atcctctcca aacccgaacg
    11821 cattacccaa atcatccgcg acgaactgga agaaatcaaa accaactatg gcgacgaacg
    11881 ccgcagcgaa atcaacccgt tcggtggcga cattgccgat gaagacctga ttccgcaacg
    11941 cgaaatggtc gttaccctga cacatggcgg ctatatcaaa acccagccga ccaccgacta
    12001 tcaggcgcag cgtcgcggcg ggcgcggcaa acaggcggct gccaccaaag acgaagactt
    12061 tatcgaaacc ctgtttgttg ccaacacgca tgattatttg atgtgcttta ccaatttggg
    12121 caagtgtcat tggattaagg tttacaaact gcccgaaggc ggacgcaaca gccgcggccg
    12181 tccgattaac aacgtcatcc agttggaaga aggcgaaaaa gtcagcgcga ttctggcagt
    12241 acgcgagttc cccgaagacc aatacgtctt cttcgccacc gcgcagggaa tggtgaaaaa
    12301 agtccaactt tccgccttta aaaacgtccg cgcccaaggc attaaagcca tcgcgctcaa
    12361 agaaggcgac tacctcgtcg gcgctgcgca aacaggcggt gcggacgaca tcatgctgtt
    12421 ctccaactta ggtaaagcca tccgcttcaa cgaatactgg gaaaaatccg gcaacgacga
    12481 agcggaagat gccgacatcg aaaccgaaat ttcagacggc atcgaagatg aaaccgccga
    12541 cagcgaaaac gcactgccga gcggcaaaca cggtgttcgc ccgtccggtc gcggcagcgg
    12601 cggtttgcgc ggtatgcgcc tgcctgccga cggcaaaatc gtcagcctga ttaccttcgc
    12661 ccctgaaacc gaagaaagcg gtttgcaagt tttaaccgcc accgccaacg gatacggaaa
    12721 acgcaccccg attgccgatt acagccgcaa aaacaaaggc gggcaaggca atattgccat
    12781 taacaccggc gagcgaaacg gcgatttggt cgccgcaacc ttggtcggcg aaaccgacga
    12841 tttgatgctg attaccagcg gcggcgtact tatccgcacc aaagtcgaac aaatccgcga
    12901 aaccggccgc gccgcagcag gcgtgaaact gattaacttg gacgaaggcg aaaccttggt
    12961 atcgctggaa cgtgttgccg aagacgaatc cgaactctcc gacgcttctg taatttccaa
    13021 tgtaaccgaa ccggaagtcg agaactgaaa atcatctccc gatgccgtct gaagattcag
    13081 acggcattta ttttatccct catccgtcat ccagcttctc acaatatagc ggattatagt
    13141 caattaaaaa caaggggctg tcctagataa ctagggaaat tcaaattaag ttagagttgc
    13201 ccctatgaga aaaagtcgtc taagccggta taaacaaaat aaactcattg aact
//
LOCUS       DAWKOG010000045        12433 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.045, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000045 DAWKOG010000000
VERSION     DAWKOG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12433
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.045"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(42..809)
                     /locus_tag="WJK66_000947"
     CDS             complement(42..809)
                     /locus_tag="WJK66_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ7578677.1"
                     /translation="MSSNKASFFTRLRRLCRLAVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGVLAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIRV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(897..1724)
                     /gene="mutM"
                     /locus_tag="WJK66_000948"
     CDS             complement(897..1724)
                     /gene="mutM"
                     /locus_tag="WJK66_000948"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEZ7578678.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQVNPDLGEI
                     LSGRQVLSCGRRAKYLIVRFQTGILLIHLGMSGSLRIFTPSDGRIGKPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEVFCADYLYARLKTQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPHRPANRLKKKECALLVETVKAVLQRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPQCGGLVVKETLGQRGTFYCPNCQK"
     gene            complement(1776..2441)
                     /locus_tag="WJK66_000949"
     CDS             complement(1776..2441)
                     /locus_tag="WJK66_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ7578679.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGRWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGIPSQILSEAHRILKPCGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRQLADVGFDIEFGKFMVYLPPVS
                     SIGQIRFWRFMEKAGDRWWPQCAAVYALVLVKRAAGVTPLPAWDGYLGGKALAAGAAR
                     VAD"
     gene            2619..4595
                     /locus_tag="WJK66_000950"
     CDS             2619..4595
                     /locus_tag="WJK66_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ7578680.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWSELRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIVPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRVRLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            4663..5079
                     /locus_tag="WJK66_000951"
                     /pseudo
     CDS             4663..5079
                     /locus_tag="WJK66_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
     gene            5079..5225
                     /locus_tag="WJK66_000952"
                     /pseudo
     CDS             5079..5225
                     /locus_tag="WJK66_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
     gene            5201..6409
                     /locus_tag="WJK66_000953"
     CDS             5201..6409
                     /locus_tag="WJK66_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ7578681.1"
                     /translation="MLVIGIPMILVENVIGRRKGVNALDAFGGSMNGKPVAKIWKLVG
                     WAGLLGAFGIMAYYMVLGGWVISYIVNIIGGNLNISSPVDGVVTKGFFTEHIENSPWE
                     IAFYTFLFVVVNQWILVKGVIGGIEKAAKYLMPLLFLFLIAMVVRNVTLPGAMEGITF
                     YLKPDFSKITTELFVFVLGQVFFALSLGFGVMITLSSYLDKNENLVQTAVITAITNTI
                     IAVLAGFMIFPSLFSFGVAPNSGPTLVFQSLPIVFSHMWAGSVFAVIFFSLLLIAALT
                     TSLTIYEVLITTIQEKTKIRRTAAITIVLAAIFIFGNIPSILSYGPWKDVSIFGKNIF
                     DAFDYISGNILFMLTALGSALFVGFVMKDEAKEELLYKGNHTTVNIWFAYVKYLVPLV
                     ILLIFVSNLF"
     gene            6653..7309
                     /locus_tag="WJK66_000954"
     CDS             6653..7309
                     /locus_tag="WJK66_000954"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEZ7578682.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVGWHDEENVSELAKKLPAVFSGSRILLGGEDVSDGIRTEAIGMGASAVAQLP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYREV
                     "
     gene            7465..9150
                     /gene="rpsA"
                     /locus_tag="WJK66_000955"
     CDS             7465..9150
                     /gene="rpsA"
                     /locus_tag="WJK66_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEZ7578683.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQDKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDESRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            9161..9475
                     /locus_tag="WJK66_000956"
     CDS             9161..9475
                     /locus_tag="WJK66_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEZ7578684.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(9826..10233)
                     /locus_tag="WJK66_000957"
     CDS             complement(9826..10233)
                     /locus_tag="WJK66_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEZ7578685.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            10381..11490
                     /locus_tag="WJK66_000958"
     CDS             10381..11490
                     /locus_tag="WJK66_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEZ7578686.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPSKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGVGQEISTRPFQLVTGRVWKGTAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINEAFELMHEGKSIRAVIHY"
     gene            11499..12326
                     /gene="fghA"
                     /locus_tag="WJK66_000959"
     CDS             11499..12326
                     /gene="fghA"
                     /locus_tag="WJK66_000959"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEZ7578687.1"
                     /translation="MKLIEQHQIFGGSQQVWAHNAQTLQCEMKFAVYLPNNPENRPLG
                     VIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVASDTSPRGEQVPNDDAYDLGQGAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEKHFPTNGKRSIMGHSMGGHGALVLALRNQE
                     RYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
BASE COUNT         3258 a         3440 c         2903 g         2832 t
ORIGIN      
        1 ccacaaaagc gaatcatact gccacattcc gacaaccggt ttcagacagc gatatccgca
       61 tcgtcggcga caacggcacg gatgctttct tcgattttat cttttaaagc ataacggtct
      121 tcgctttccg ccgcatccgc cacgcaaacg aaatcgactc ttatcgtcaa ttttttcata
      181 gacacgatgc gccacaggca ggtcggcaaa ccgacatcgg catatgaggg acgagccgtc
      241 ctttttcccg tttcgtcata ataacgcagc gcgaccgcca aaacctttgc ccccgcatcg
      301 atggcggatt ggaacagcgc ggctttgaac ggcaaaagcc ccaatccgga ggaagtccgc
      361 gcttcgggga aaaaactgac gttttgaccg cgttgcaagg tttcgcagac ggcgcggtta
      421 atcggttcga tgtcgcgccg cgaattgcgg ttgatgaaca ccgttcccgc gttctgcccc
      481 atcttgccca ataccggcca gcttttgatt tcctgcttgg cgataaagct gctcggataa
      541 accgcgctca tcgcgaaaat atccagccag gacacgtggt tggcggcaac caagacaccg
      601 ttcggatgtt cgggtgcggg tctgcccacc tccaatccga tatccaaagc cgccaaaacc
      661 cccctgccca actcgattac cgcccgattg cgcgactcgg ggcaaccgcc gtcaataccg
      721 cgcaggtttt tcccggtttt gaacagccag accgccaaac ggcacaagcg gcgcagacgt
      781 gtaaaaaatg aagctttatt tgaagacatg agcaattctt tcccgataat cggtgtttgt
      841 tgttgaaacg ttcggacggc ataccgataa aatgccgtct gaaaccgttt tcagtcctat
      901 ttctgacagt tcgggcaata aaacgtgccg cgctgcccca aagtttcttt cacaaccaaa
      961 ccgccgcact gggggcacgg ctgattgtgc cgcccgtaca ctgtatattc ctgttggaag
     1021 tagccgcttt tgccgtcgct gtccacaaaa tccctcaagg tactgccgcc cgtttcaatg
     1081 gcgcgctgca acaccgcttt gacggtttca accaaaagcg cacactcttt ctttttcagg
     1141 cggttggcag gacggtgggg cgaaatgccc gccctaaaca ggctctcgtt ggcataaatg
     1201 ttgcccacac cgaccacgac cgcattgtcc atcagggcca gcttgactgc ccgtttctgg
     1261 gttttcagcc ttgcatacag ataatccgca caaaatactt ccgacagagg ttcaggcccc
     1321 agtttttcca acagcggatg atgttcttcg attccctcat accaaagtat cgcgccgaac
     1381 tttctcggat cgcggtaacg catgaccgtg ccgtctgaaa acacaatatc gacgtgatcg
     1441 tgtctgtccg gcttgccgat acgtccgtcc gacggcgtaa aaatccgcaa actgcccgac
     1501 atccccaagt gaatcagcaa aatccccgtt tgaaaacgga caatcagata tttcgcccgc
     1561 ctgccgcaag acaaaacctg cctgccggac aaaatctccc ccaaatcggg attgacctgc
     1621 cagcgcagct tcaattggcg caataccacg gcttccaccg tttttccttc aatatgcggc
     1681 gcgatgccgc gcaacgtcgt ttccacttcc ggcaattcag gcataacccc tcccgacatt
     1741 ttttctgaca gatgccgtct gaaagacggc cgtccctaat ccgcaaccct tgccgcaccc
     1801 gccgcaaggg ctttgccgcc caaatacccg tcccacgcgg gcaggggcgt tacgcccgcc
     1861 gcccttttga ccaaaaccaa agcgtacact gcggcacatt gcggccacca acggtcgccc
     1921 gccttttcca taaaccgcca aaagcgtatt tgcccgatcg acgaaacggg cggcagatac
     1981 accataaatt tcccaaattc aatatcgaaa ccgacatccg caagctgcct tttcaactcg
     2041 ggcagcggca gacaaaaccg tttttccggc aggcgttcgc cgtcaaacca acggctgaat
     2101 ccccagagcg aatacggatt gaaacccgtc agcatcaagc gtccgcacgg tttcaatatc
     2161 cggtgcgcct cggacaggat ttgcgaaggg ataccgcctt caagcgtatg cggaaaaagc
     2221 agcatatccg cagaaacatc cgccaaagcc atattctccg ccgacatcga catatctcgc
     2281 ggcacacaga caacatcttc agacaggctc agccacctgc cgcccacctg aaccgcacac
     2341 attcccgaaa aacggtatga atccagatac cgcccgaaga aatcctgttc caattttgca
     2401 acataccgcc ccatcgccgt atcttcaaac catgcatcca tattgcgtcc gtttcaaaca
     2461 gattgcctgc cgatattcta ttccaaacag gatttctgtc aaaaaacaca tcggccgccc
     2521 atttccgaat ccgcataaag ttcctgtaaa acttgacgct ttttcagtca aacagtacca
     2581 tcggacgata aaatatgttt attccgccga tataaatcat gtccaaactc aaaaccatcg
     2641 ccctgaccgc gtcaggtctg tccgtttgtc cgggtttcct atacgcccaa aacacctcat
     2701 cacaccaaat cggtttggcg attatgcgct taaactcttc aatactcgac ctgcccccga
     2761 caaaacaata tttccaatcc ggcagcctgt ggagcgagct gcgccaaggc ttccggatgg
     2821 gcgaagtcaa tcccgaactg gtacgccgcc acgaaagcaa attcatcgca agccacagct
     2881 atttcaacag ggtcatcaac cggagtagac cctatatgta ccatatcgcc aacgaagtca
     2941 aaaaacgcaa tatgcccgcc gaagccgccc tgcttccctt catcgaaagc gcgttcgtca
     3001 ccaaagccaa atcacacgtc ggcgcatcgg gcctgtggca gttcatgccc gctaccggca
     3061 ggcattacgg cctggaaaaa acaccggttt acgacggcag gcacgacgtt tacgccgcca
     3121 ccgatgccgc actcaactat ctgcaatacc tctatggact gttcggcgac tggccgcttg
     3181 cctttgccgc ctacaactgg ggtgaaggca acgtcggacg cgccatcaac cgcgcccgcg
     3241 cccaagggct cgaaccgacc tacgaaaacc tgcgtatgcc caacgaaacg cgcaactatg
     3301 tccccaagct gctcgccgtg cgcaacatta ttgccactcc ccaatctttc ggcatgaata
     3361 tcagcgacat agacaacaaa ccctattttc aggcagtcga accggatcgt ccgctcgaca
     3421 acgaagccat cgcccggctt gccggcatca cgcaaagcga gctgctcgcc ctaaaccccg
     3481 cattcaacgt ccccgcgttt atccccaaaa gcaaacgcaa actgctgctt cctgtcgcgt
     3541 ccgtacaaac cttccaaagc aactacctca acgccgcacc cgacagcctg ttttcatggg
     3601 aagtctatac gcctgccgcc aaaaccagcc tgtccgacat ctcgacggca accggcatga
     3661 gcattgccga catcaaacgc ctcaacaacc tgaacggcaa ccttgtcaac gcaggacgca
     3721 gcatccttgt cgccaagaac ggcaaaaccc ttcagacggc atcggaatcc gtcgtttcca
     3781 tcgacatcga caatacaccc gacacctacc gttccaatat gccggcaggc acggtgaacg
     3841 tcggcattgc ccgaatccga cccgccgccg cacagacagc ggacattacc gtcgcacctt
     3901 tgccgcagaa aaccgtccgt acggaacccg atccccttgt ccgtattgcc gaacctgccc
     3961 ttgcgacagc cgcagcgcaa cctcaaaccg aaaaacagac cgccatgccg tctgaaaccc
     4021 aaaccgcaac actcgcgcag atcgtccccc aaaacgacat gcaggcggca gacgaactca
     4081 tgcagcttgt tgcccgaaac aacctgcgcc gacaggctga agaaaccatc tctgccgtca
     4141 tcggcacgcc tgacacagtt gccgaacaca acatttcctc atctccgcaa cataccgttg
     4201 ccgccgacgg caaacgccgg gtacgtttgg aaacgcgcgt agccaaagct gccgacggcg
     4261 aagccgaaat ctccccgctc catgccagca tccaccgcgt tgtagaaggc gacaccctgt
     4321 tcaacattgc caaacgctac aacgtcagtg tagccgacct gattgtcgcc aacaacatca
     4381 aaggcaacac catccaaaaa ggacaggtac tccgcctggc gcaggcagcc cctgcccaaa
     4441 cccgtattga aaaagtatcc tacaccgcgc gcaaaggcga caccttcaaa agtatcgccg
     4501 cgcgcttcaa tatccatatc gacgacatcc gccggctcaa tcccaacctg aacaccatca
     4561 atccgggaca gagggtcaaa ctgattggaa gctgattcgg atacggcaca tgacaggact
     4621 ttctcagtcc tgtctttttc atcccacatt tcccatacca tcatgaaact tatcaaatac
     4681 ctgcaatatc aaggcatagg aagccgcaag cagtgccaat ggctgattgc cggcggttat
     4741 gttttcatca acggaacctg catggacgac accgatgcag acatcgattc ctcatccgtc
     4801 gaaacgttgg atattgacgg ggaagcagta accgtcgttc ccgaacccta tttctacatc
     4861 atgctcaaca agcctgaaga ttacgaaact tcgcacaaac ccaagcacta ccgcagcgta
     4921 ttcagcctgt tccccgacaa tatgcggaac atcgatatgc aggcggtcgg caggctggat
     4981 gcggatacga ccggcgtatt gctgattacc aacgacggca aactgaacca caacctgact
     5041 tcgccgagca gaaaaattcc caagctatac gaagtaacca ccatcaagcc tttatcctga
     5101 ccattgccgg cgcgactgtc ggtttcggcg cgacgtggcg tttcccgtat ttggtcggtg
     5161 aaaacggtgg tgcgcgtatg tgtttttatt ctgtatcgcg atgctggtta tcggcatccc
     5221 gatgattttg gtggaaaatg tcatcggacg gcgcaaaggc gtgaacgcgc tggatgcgtt
     5281 cggcggttcg atgaacggca aacccgtcgc taaaatttgg aagctggtcg gctgggcagg
     5341 actgctcggc gcgttcggca ttatggctta ttacatggta ctcggcggtt gggtcatcag
     5401 ctatatcgtt aatattattg gcggaaatct gaatatttcc agccccgtcg acggtgtggt
     5461 tacaaaaggc ttttttaccg aacacattga aaacagccct tgggaaattg cgttctacac
     5521 tttcttgttt gtcgttgtga accaatggat tttagtcaaa ggcgttatcg gcggtattga
     5581 aaaagcggca aaatacctga tgccgctgct gtttttgttc ctgattgcca tggtcgtacg
     5641 caacgttacc ctgcctggcg caatggaagg gattactttc tatctgaagc ctgatttcag
     5701 caagattacc accgaactgt tcgtcttcgt tttgggacaa gtatttttcg ccttgagctt
     5761 gggtttcggc gtgatgatta ccttatccag ctatctggat aaaaacgaaa atctggttca
     5821 gacggcagtt atcacggcaa ttaccaatac catcatcgcc gtacttgcgg gctttatgat
     5881 tttcccgtcg ctcttcagct tcggcgtcgc gcccaattcc ggcccgactt tggtgttcca
     5941 aagtttaccg attgtgttct cacatatgtg ggcaggatct gtgttcgccg tcattttctt
     6001 ctctctactc ctgattgccg cgttgacaac ttcgctgacg atttatgaag tgttgattac
     6061 gaccattcag gaaaaaacca aaatccgccg caccgccgcg attacgattg tattggctgc
     6121 catcttcatc ttcggcaaca tcccgtccat tctgagctat ggtccgtgga aagatgtttc
     6181 catatttggc aaaaatattt ttgacgcttt tgactacatc agcggcaaca tcttgtttat
     6241 gctgaccgcg ctcggctccg cgctgtttgt cggttttgtg atgaaggacg aagcgaagga
     6301 agaattgctt tacaaaggca accatacgac ggtcaatatt tggtttgctt atgtgaaata
     6361 tcttgtgccg ttggtgattt tgctgatttt tgtcagcaac ctgttctaat ctgcagcaat
     6421 cgatgccgtc tgaaggccat acctctttca gacggcattg ccttgatgcc cgccacaata
     6481 cccgcaacgc cggaatccga accggcttgc cggcttctgt cggatgtttt cgggcaggcg
     6541 gcgttccgtc tgcttagaca atcgtccttt aaaacaggta gaatccgccc caacgggaaa
     6601 cacatccttc agacggcaaa acccataccc caaaccatca ggaatccccc ttatgaacag
     6661 acaaaaagtc atcgccatcg acggcccggg cgcatcgggc aaaggcacgg ttgccgcccg
     6721 cgttgccgcc gcattgggat acgattatct cgataccggc gcactctacc gcctgaccgc
     6781 cctatatgca caaaaacaag gcgtgggatg gcacgatgaa gaaaacgttt ccgaactggc
     6841 aaaaaaactg cccgccgtat tttcaggcag ccgcatcctg ctcggcggcg aagacgtttc
     6901 agacggcatc cggacagaag ccatcggcat gggcgcatcc gcagtcgcac agttgcctaa
     6961 agtccgcgcc gccctgctgc aacgccaacg cgattttctg accgaaaaag gactggttgc
     7021 cgacggacgg gacaccggat cggtcgtctt cccccaagcc gaacttaaaa tcttcctgac
     7081 ggcagaatcc aaaatccgtg ccgaacgccg cgccaaacaa atcggcatcc cctgcgaagg
     7141 tttggcattc gagcgcatcc tgtccgacat cgaagccaga gacgaggcag accgaaaccg
     7201 caaagttgcc cccctgaaac aacagcccga tgccctgctt ttggacacaa gccgcctgac
     7261 tatagaagaa actgtaaaaa aagtgcttga ttggtatcgt gaagtttaaa ttttcaggta
     7321 taatcgcaca aattacgttt cagacggcat aaaaatcccc catatgccgt ctgaaacctt
     7381 atgtacccgt ctgccctgcc aaggacggca gatatccacc aacccacccc gcaccccttg
     7441 gcggtgtacc gaaaagagtt atatatgtct atggaaaatt ttgctcagct gttggaagaa
     7501 agctttaccc tgcaagaaat gaacccgggt gaggtgatta ccgctgaagt agtggcaatc
     7561 gaccaaaact tcgttaccgt aaacgcaggt ctgaaatcag aatccctgat tgatgtagct
     7621 gaattcaaaa acgctcaagg cgaaattgaa gttaaagtcg gcgacttcgt taccgttacc
     7681 atcgaatccg tcgaaaacgg cttcggcgaa accaaactgt cccgcgaaaa agccaaacgc
     7741 gctgccgatt ggattgccct ggaagaagcc atggaaaacg gcaacatcct gtccggcatc
     7801 atcaacggca aagtcaaagg cggcctgacc gttatgatta gcagcatccg cgcattcctg
     7861 ccgggttctt tggtcgacgt acgtcctgta aaagacactt ctcacttcga aggtaaagaa
     7921 attgaattca aagtgatcaa actggacaaa aaacgcaaca acgttgttgt gtcccgccgc
     7981 gccgttctgg aagctacctt gggtgaagaa cgcaaagccc tgctggaaaa cctgcaagaa
     8041 ggctccgtca tcaaaggcat cgttaaaaac attaccgatt acggtgcatt cgttgacttg
     8101 ggcggcatcg atggtctgct gcacatcacc gacttggcat ggcgccgtgt gaaacacccg
     8161 agcgaagttc tggaagtcgg tcaagaagta gaagctaaag tcctgaaatt cgaccaagac
     8221 aaacaacgcg tttctctggg tatgaaacaa ctgggcgaag atccttggag cggcctgacc
     8281 cgtcgttacc cgcaaggtac ccgcctgttc ggtaaagtat ccaacctgac cgactacggt
     8341 gcgttcgttg aaatcgaaca aggcatcgaa ggcttggtgc acgtttccga aatggattgg
     8401 accaacaaaa atgtacaccc gagcaaagtt gtacaattgg gcgacgaagt cgaagtaatg
     8461 atcctcgaaa tcgatgaaag ccgccggcgt atctctttgg gtatgaaaca atgccaagcc
     8521 aatccttggg aagaatttgc cgccaaccac aacaaaggcg acaaaatctc cggcgcggtt
     8581 aaatccatta ccgatttcgg cgtattcgtc ggtctgcctg gcggcatcga cggtttggtt
     8641 cacctgtccg acctgtcctg gaccgaatcc ggcgaagaag ccgtacgcaa atacaaaaaa
     8701 ggcgaagaag tcgaagccgt cgtattggca atcgacgtgg aaaaagaacg catctccttg
     8761 ggtatcaaac aactggaagg cgatccgttc ggcaacttca tcagcgtgaa cgacaaaggt
     8821 tctttggtta aaggttccgt gaaatctgtt gacgccaaag gtgctgttat cgccctgtct
     8881 gacgaagtag aaggctacct gcctgcttcc gaatttgcag ccgaccgcgt tgaagatttg
     8941 accaccaaac tgaaagaagg cgacgaagtt gaagccgtca tcgttaccgt tgaccgcaaa
     9001 aaccgcagca tcaaactttc cgttaaagcc aaagatgcca aagaaagccg cgaagcactg
     9061 aactccgtca atgccgccgc caatgcgaat gccggcacca ccagcttggg cgacctgctg
     9121 aaagccaaac tctccggcga acaagaataa ggttgcagac atgacaaagt ctgagttaat
     9181 ggttcgtttg gcagaagtgt ttgccgccaa aaacggcacg catcttctgg caaaagacgt
     9241 agagtacagc gtaaaagtct tggttgacac catgactaga tcgcttgccc gaggtcaacg
     9301 catcgaaatc cgcggtttcg gcagcttcga tttgaaccat cgtcctgccc gcatcggtcg
     9361 caatcccaaa accggcgagc gtgtggaagt acctgaaaaa catgtacccc acttcaagcc
     9421 cggtaaagaa ttgcgcgagc gggtcgactt ggctttaaaa gaaaatgcca attaaacctt
     9481 agcatcaaaa cgccgctgtt acgcggcgtt ttttctgtgg tttaacttca tccgttgctt
     9541 caataccttg agccaagcaa gcaacggatt agagcgtgga tttttttata gtggattaac
     9601 aaaaaccagt acggcgttgc ctcgccttag ctcaaagaga acaattctct aaggtgctga
     9661 agcaccaagt gaatcggttc cgtactattt acactgtctg cggcttcgtc gccttgtcct
     9721 gatttttgtt aatccactat atcattgctt acaatccgct ttttaaacaa caaatttttg
     9781 atttctatta cgaacaggac aaaaatcctg cttattgcac taaaactaag ccgtttcagg
     9841 aatttgcggc aaatttacag cttttaccga gcctaatgct ttcgcttttt ggtaaaacgc
     9901 caatttgtat tcaagcaaat ctaaatagcg ttttaattcg gcaatttgac acttcacatt
     9961 ttctatttga ttttcaaaca aggaaaggcg ttcttcaatg gtatcgtcgc caatgacggt
    10021 acattccgca aagcgtttga tgtcttttaa gctcatcccc gtatttttca agcattgcaa
    10081 taagcccaac cattgcaaat cgttatcggt aaaacagcgg ttaccgtatt catcacgtcc
    10141 gatattgggc aacaaacctt ctttgtcgta aaaacgtagg gtgtgggcgg agatgcctat
    10201 tttttcggcg gctttggcag tagtataagt cattttccct ccttctaaac aaaaacagta
    10261 aaaaacactt gctttagagt taactctaaa gtgtaaactg ttgctatgtt gctcaggcaa
    10321 ggcaactttg tcaatgaatt aagaggaaag acaatggaaa tgaaacaagc cgattcaacc
    10381 atcaaatctc gtgcggcggt ggcattcgcc ccaaatcaac ccttacaaat tgtggaaatc
    10441 gacgtagaaa tgccgcgtaa aggcgaggtg ttaatccgca atacccacac tggcgtgtgc
    10501 catactgatg cgtttacgtt atcaggaagc gatcctgaag gcgtattccc tgtggtgctt
    10561 ggacacgaag gtgcgggtgt ggtcgttgct gtgggcgaag gtgtgtcaag cgtaaaaccc
    10621 ggtgatcacg tgattccgct ttacaccgcc gaatgtggcg aatgtgagtt ttgttgttca
    10681 ggtaaaacca acttgtgcgt ctcagtgcgt gatacacaag gtaaaggctt aatgccggac
    10741 ggcacgacgc gtttttctta tcaaggtcag ccgatctatc actatatggg ctgttcgact
    10801 ttcagtgaat actcagttgt tgcagaagtt tcactggcaa aaattaaccc ggaagcgaac
    10861 catgaacaag tatgtttgct cggctgcggc gttactacag gtattggcgc ggtgcataat
    10921 acggcaaaag tgcaagaagg cgactctgtg gcagtgtttg gcttgggagc gattggtttg
    10981 gcggttgtgc aaggtgcgcg tcaagccaaa gccggccgca ttatcgccat tgataccaat
    11041 ccctcaaaat tcgagttggc aaaacagttc ggtgcaacgg actgtttgaa cccgaacgat
    11101 tacgataaac cgatcaaaga tgtgttgtta gacatcaaca aatggggcat tgaccatacc
    11161 tttgaatgta tcggcaatgt aaacgtaatg cgtcaagcat tagaaagtgc acaccgtggt
    11221 tggggacaat ccattatcat cggcgtagca ggtgtaggac aagaaatttc aactcgtccg
    11281 ttccagttgg taacaggccg tgtgtggaaa ggcacggcat ttggtggcgt gaaaggccgc
    11341 tctgaactgc cgaaaatggt ggaagattca atgaaaggcg acattgagtt agaaccgttt
    11401 gtaacccaca caatgacact cgatcaaatc aatgaagcct ttgagttaat gcacgaaggt
    11461 aaatcgatcc gcgccgttat tcactactaa ggtatgcgat gaaactgatt gaacaacatc
    11521 aaatttttgg cggttcgcaa caagtttggg cacacaatgc ccaaacgctt caatgcgaaa
    11581 tgaaatttgc cgtctatttg ccaaataatc cagaaaatcg accgcttggt gtgatttatt
    11641 ggctttccgg cttgacgtgt acggaacaaa atttcattac caaatcaggc tttcagcgtt
    11701 atgcggcaga acatcaagta attgtggtgg cctccgatac cagccctcgc ggagagcaag
    11761 tgccgaacga tgatgcttac gatttaggac agggtgcagg cttttatttg aatgcgaccg
    11821 aacagccttg ggcggcgaat tatcaaatgt atgattacat tttgaacgag ctgccccgtc
    11881 tgattgagaa acactttcct accaacggca aacgttccat tatggggcat tcaatgggcg
    11941 gacacggcgc attggtattg gcgctgcgga atcaggaacg ttatcaaagt gtttctgcct
    12001 tttcgcctat tttatcgcca agcctcgtgc cgtggggaga aaaagccttt actgcttatt
    12061 tagggaaaga ccgtgaaaaa tggcagcaat atgatgcgaa ctcactcatt caacaaggct
    12121 ataaagtgca aggtatgcgc atcgatcaag gcttggaaga tgagtttttg ccgacacaat
    12181 tgcgtaccga agattttatc gaaacctgcc gtgcggcaaa ccagccggtc gatgtgcgtt
    12241 tccataaagg ctacgatcac agctattact tcatcgccag ttttattggc gagcatattg
    12301 cttatcacgc cgcgtttttg aagtaaacca aagagcgttc agtgttcaaa gcagttttgg
    12361 gatagccggc acgagggcgg taagaagtgc cggcataaac gtatgccgtc tgagccggaa
    12421 gaagccgact cta
//
LOCUS       DAWKOG010000046        12428 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.046, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000046 DAWKOG010000000
VERSION     DAWKOG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12428
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.046"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            555..1730
                     /locus_tag="WJK66_000960"
     CDS             555..1730
                     /locus_tag="WJK66_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEZ7578688.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIKRESKLQTNAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(2071..2619)
                     /locus_tag="WJK66_000961"
     CDS             complement(2071..2619)
                     /locus_tag="WJK66_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEZ7578689.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNAGSK"
     gene            complement(2622..4118)
                     /gene="ccoG"
                     /locus_tag="WJK66_000962"
     CDS             complement(2622..4118)
                     /gene="ccoG"
                     /locus_tag="WJK66_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEZ7578690.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     AAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            4646..6625
                     /gene="tkt"
                     /locus_tag="WJK66_000963"
     CDS             4646..6625
                     /gene="tkt"
                     /locus_tag="WJK66_000963"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEZ7578691.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEQGAKLEADWNELFAQYQAKYPAEAAEFVRRMDKKLPDNF
                     DEYVQAALKEVCAKAETIATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLVLHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVTWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLNDIKRGGYVISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVHVVSMPSTNVFDRQDTAYQAAVLPESLPRIAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            6766..8154
                     /gene="fumC"
                     /locus_tag="WJK66_000964"
     CDS             6766..8154
                     /gene="fumC"
                     /locus_tag="WJK66_000964"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEZ7578692.1"
                     /translation="MNTRTEHDTMGNVEVLSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGKLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNEHCAVGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(8219..9142)
                     /locus_tag="WJK66_000965"
     CDS             complement(8219..9142)
                     /locus_tag="WJK66_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ7578693.1"
                     /translation="MENQRPLLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKWRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAVGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAELAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYAGALVVVGGAVTAAVGDRLFKRR"
     gene            9314..9454
                     /locus_tag="WJK66_000966"
     CDS             9314..9454
                     /locus_tag="WJK66_000966"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578694.1"
                     /translation="MPSENSGDVRQASLPQNARTAQSQRSAQRKGRQQAGSDAKKRNP
                     AP"
     gene            complement(9514..10038)
                     /locus_tag="WJK66_000967"
     CDS             complement(9514..10038)
                     /locus_tag="WJK66_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEZ7578695.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
     gene            complement(10042..11424)
                     /locus_tag="WJK66_000968"
     CDS             complement(10042..11424)
                     /locus_tag="WJK66_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ7578696.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLIVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFLLTGILTVASIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFILGIACIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGVAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQVNQEVLYRALSELEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            complement(11535..12158)
                     /locus_tag="WJK66_000969"
     CDS             complement(11535..12158)
                     /locus_tag="WJK66_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ7578697.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            12218..>12428
                     /locus_tag="WJK66_000970"
     CDS             12218..>12428
                     /locus_tag="WJK66_000970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578698.1"
                     /translation="MLPTRKTARKIQARHIALPFSGKPQFYRPQERLSKQAQATPPGH
                     FRFHRLSVVRIMQQHHQRITLFGRQQ"
BASE COUNT         3055 a         3620 c         3076 g         2677 t
ORIGIN      
        1 attcccaatc cctgagatta tccctcgggg atttcttatt acctcgacaa ctcgttacca
       61 tatctttacc ccccttcatc agactctcag acgtaatcga atcatattca aacctttgcc
      121 atgcaaaccg atatcacgta accggatgcg gtgtccgtcc aacattttac ccgattgaaa
      181 cgcctgatat attgcacccc atcaacgtgg cattactttt cttaacaatc ccctttgaca
      241 gcaactgact agggcttttt tatgccatca tcaaatttat agtggattaa ctttaaacca
      301 gtacggcgtt gcctcgcctt tccgtactat ttgtactgtc tgcggcttcg tcgccttgtc
      361 ctgatttttg ttaattcact ataatatttt ctctcccgat tgaaacaggc gtaacagaat
      421 atgcccgaag ctccggctgc tttcttgttt accgccgcga tatttagagt ataataccaa
      481 atttgagcaa tagttctaaa acagttagaa ccatttttca tgagcctgac tgattcgtac
      541 actcggagaa actgatgcag aatatttttg accctttggt tattcgtgga aaatccctta
      601 cccccatcgt gcaaggcggt atgggggtcg gtgtttccgc atcgggttta tccagcgcgg
      661 tggcacgtga aaacggtatc ggaacgattg ccagtgtgga tttgcgccac cttcacgaag
      721 acctactcgc cgaatcacaa atcaatccga gtgaagagaa atatacatct ttgaactgta
      781 ccgcattaga cagggaaatc caaaaagcca aaagcgcttc agagggaaaa ggactgattg
      841 cggtcaacgt gatgaaggcg gtcaaagacc acgccgcgta tgtccgccag gcttgcgaat
      901 caggggcgga tgcggttgta atgggtgccg gtctgccttt agacctgccg gaaatgaccg
      961 agggctatca taaagatgtc gcgctgctgc cgattctgtc cgaatcgcgc ggtattaata
     1021 tcgtcttgaa acgttggatg aaaaaaggca tattgcccga tgcgattgta gtcgaacatc
     1081 ctgcccacgc ggccggacat ttgggtgcat caaccgttga aggcgtaaac gatgccaagt
     1141 tcgacttcaa acgcgtgatt gaggaaacgt ttgaagtttt caaaagttta gggctggaaa
     1201 gcgaaaaaat cccgcttatt cttgcgggag gcatggcaaa ttttgaaaaa gtcaaaaccg
     1261 ccctaaagaa ctggggagca tccgccgttc aaatcggtac ggcttttgcc gttaccgaag
     1321 aaggagatgc acaccttaac ttcaaaaaaa cgctcgccgg tgcggaaact gaaaaagtag
     1381 tcgaatttat gtctgttgcc ggtttgccgg cgcgcggtgt ccgcaccaaa ttcctagaca
     1441 gctacatcaa gcgtgaaagc aaacttcaga caaacgccaa agccgacccg cgccgctgta
     1501 cccaaggttt aaactgccta accagttgcg gtctgcgcga cgggctttcc aaagccggac
     1561 aattctgcat tgatatccag cttgccgccg cattccgtgg agaagtagat aaaggcctgt
     1621 tcttcagagg taaagacccg ctgcccttcg gcaatgccat ccgcaccgtc cgcgagacga
     1681 tacaatatct gctgacgggg agcgaacctg ttgcaacgct cggacgctga catcaggtag
     1741 gatttgattt gcaaacaaaa tgccgtctga agcctttcag acggcatttt tcaggctgcg
     1801 tttcggaata gtgtcgaaaa aacggagaga aataaaaacg tttttatgta aacaaaacgc
     1861 cttccgcatt gtgtcgggca tacaacacaa atgccgtctg aagcccttca gacggcattt
     1921 ttcaggctgc atttcggaat ggtgttaaaa aataaacggg ataagataca tttattttcg
     1981 tccgataaat cagaccatca cgccgaataa tcaaacaatg cctgcccgac attgcatctg
     2041 gcaaaccaat gcaatgaaaa cacggctttt ttatttgctt ccggcattat tgaaaagttt
     2101 atccatcggg gtcagatcca ccgcattgcc ttggctggta atccatttgt tctcgacgcg
     2161 ccacacgcct gccgcgtcct ccacgcgcac ataccagtgg ttggtcggcg aaagggtttt
     2221 gaacaccgcc tcatattccg ccctgccgtt ctgcgcgctg ccgacgggct tgagggcgac
     2281 ggtttgatcg tccgccttgc gggtcgggtg catcagcagc aggttcaaag gctgtttgcc
     2341 gtcaaactcg ccgccgacaa acacttttgc cgcattcata tcgggggaaa tgagaacctg
     2401 caccccgata tgccgtctga cagcctcttc atcccgatga agctggatgt cgatatgttt
     2461 gccgtcttta taataatcgt ccgtaaccaa atctgtcgcg tgctgctgtg cgacaaaaaa
     2521 catagcgacg ctggcgatga cgacaaaaat cggccccgcc atcaagatcc acggccagac
     2581 gtgtttgtac caaggtttga ttggagtgtt ttgagacacg gttattctcc gataaaggtt
     2641 gcatcttctt ccaagacgac cggcttgccg tcgggcgcgc cgctttcgcg gtattggaag
     2701 gtaaattcga tagggtggct gcctttgtcc gcgtattccg gaatggtgga cacttggacg
     2761 ggaagggtta ccgtttcgcg cggggcaacc ttgataccgc cttcgggcag cccggtcagg
     2821 gcgatttcgt caaagccttt gacacttgcg gtaatgagct gttctttttc acttttgttg
     2881 atgatacgca ggctgtatgc gttttccagc cagcctttgg cgttttcgcg caccagtacg
     2941 ccacggtctt tcaaaatatc gacctcgacc attttgcgcg tggacaaacc ggccaggaag
     3001 gcaatgataa ctaacgccaa caccgcgccg taacctgcca cgcgcggtct gagcagccgt
     3061 tttttaatgt ctttttcaga atattcgtgt tccagcgcgc tttcggtcgt ataacggatt
     3121 aatccgcgcg gatagcccat tttgtccata atctcatcgc acgcgtcgat acaggcggcg
     3181 cagccgatac attggtattg cagaccgttg cggatgtcga tgccgacggg gcagacttgg
     3241 acgcacatcg cacagttgat gcagtcgccc aaacccgcct cttccttatt gaccgttttc
     3301 ttgcgcgcgc cgcgcggttc gccgcgttcc gcgtcataag aaacaatcag cgtgtccttg
     3361 tcgaacatcg cgctttggaa acgtgcatac ggacacatat gcaggcatac tttttcacgc
     3421 ataatgtggg cgaagaagaa ggtcataaag ccgtaaaacg ctgcggcaaa catcgcgccg
     3481 ccacctgctg ctccagtgaa taaatcggga acgaactggc ggatagggac aaaccagcct
     3541 gcaaacgtga tgcccgtcca cgcgcagaca aggaaaatca gcaggtattt ggtggctttg
     3601 atgcggattt tagtgaaatt ccacggcgat ttttccagtt tcagccgttt gtttctatcg
     3661 ccttcgacca ggttgtcaat ccacagcata atttcggtgt aaaccgtttg cgggcaggaa
     3721 tagccgcacc acagtcgccc tgcaatcgtc gtccaccaaa acagcccgaa ggcgcaaatc
     3781 atcagcagca aggcaaggta aatcaaatcg cccaccccca acgacaatcc gaaaatgaag
     3841 aaatgccgtt cggggatatt gaaaacgacg gcctgcctgc cgctccagtt gaaccacgga
     3901 atgacgtaaa acacaaactg cgtcgccaat acggcggcga tacgcagttt ggcgaaccgt
     3961 ccttccgcct ttttgggatg gatgcgttcg ccttcgggat ggatttgaat cacgctggct
     4021 cgcggatcga atgttttttt tgctttcggc gcggcttttg tttgttcgga cgtgccgatt
     4081 ccggatgccg gactgccggc ttggttttcc gtggtcattc tgcattcctt agatttttga
     4141 ttgatggttt gcccgttacc gccgccgttt gcttttcaga cgtcattttt cttgtttttt
     4201 aaggcgttgt gtttcaagtt ttgagaaaat ccgtttttcc caaaatatat ttccgctatt
     4261 gtacaacttt atgcgccgtc cggatgtatg gggcggatac atttcccatc cgcatcaaaa
     4321 cgcctggatt ttaccttacc gcccgaacaa aatccgaata cggttaaaaa aaaagactaa
     4381 aaaaaccgac acccccatat cggcagaacc gacggcgcaa gctcataaac aaacgctatc
     4441 gacaatccgg cacacaatct ataacttttt atttcaaaag gaataatggc aggcttcgcc
     4501 cgcaaatcga aaatccttcc ccgcctgtcc cctgccgccg ccttcccacg cgtccgccct
     4561 tttcttgaaa gcataagcga atcgggcgat aatcaacgct ttccgattat ccacttatct
     4621 gaaacaccag caaggaaaat acaaaatgtc tcaactggca aacgcaatcc gcttcctctc
     4681 ggccgatgcc gttcaaaaag ccaattccgg ccaccccggc gcgcctatgg gtatggcgga
     4741 aatggcggaa acattgtgga cgaaattcct caatcacaac cccgccaacc ccaaattcta
     4801 caaccgcgac cgcttcgtcc tctccaacgg ccacgcgtct atgctgttgt acagcctgct
     4861 gcacctgacc ggctacaatc tgagcattga agacttgaaa aacttccgcc agctgcacag
     4921 caaaaccccc ggccaccccg aatacggcta caccgacggc gtggaaacca cgaccggccc
     4981 gttggggcaa gggattgcca acgcggtggg tatggcattg gcagaaaaaa tccttgccgc
     5041 cgaatttaat aaagacggtt tgaacatcgt cgatcattac acctacgtct ttatgggcga
     5101 cggctgtctg atggaaggcg tatcgcacga agcctgttcg ctcgccggca ccttgggctt
     5161 gggcaaactg attgttttgt atgatgacaa caatatttcc attgatggta aagtggacgg
     5221 ctggtttacc gaaaacatcc cgcaacgctt tgaaagctac ggctggcacg tcgttcccaa
     5281 tgtaaacggt catgacaccg ccgccattca agccgccatc gaagccgcac gtgccgaaac
     5341 cggcaaaccg tccatcatct gctgcaaaac cttaatcggc aaaggcagtg ccaacaaaga
     5401 aggcagccac aaaacccacg gcgcaccttt gggcgcggac gaaatcgaag ccacgcgcaa
     5461 acatttgggc tggacttacc ccgcctttga aatcccgcaa gaaatttacg atgcgtggaa
     5521 tgccaaagaa caaggcgcga aactggaagc cgactggaac gaactgttcg cgcaatatca
     5581 agccaaatat cctgccgaag ccgcagaatt tgtgcgccgt atggataaaa agctgccgga
     5641 caatttcgat gaatacgttc aagccgcatt gaaagaagtg tgcgccaaag ccgaaaccat
     5701 cgccacccgc aaagccagcc aaaacagcat cgaaatcttg gcaaaagagc tgcccgaact
     5761 ggtgggcggc tctgccgacc tgaccccgtc caacctgacc gactggtcaa acagcgtttc
     5821 cgttacccgc gacaaaggcg gcaactacat ccactacggc gtgcgcgagt tcggtatggg
     5881 cgcgattatg aacggtttgg tattgcacgg cggcgtaaaa cccttcggcg cgactttcct
     5941 gatgttcagc gaatacgagc gcaacgccct gcgtatggct gcgttgatga aaatcaaccc
     6001 tgtatttgtg ttcacccacg attccatcgg tttgggcgaa gacggcccga cccaccagcc
     6061 gattgagcaa accgccaccc tgcgcctgat tccgaatatg gacgtatggc ggccgtgcga
     6121 caccgccgaa tccttggtca cttgggcaga agccgtcaaa gccgccgatc acccgtcctg
     6181 cctgattttc agccgtcaaa acctgaaatt ccaagcgcgc agcgagcaac aactgaacga
     6241 catcaaacgc ggcggctacg tcatcagcga agcacaaggc aacgcccaag ccgtcatcat
     6301 tgccaccggt tccgaagtcg agctggcttt ggaagcgcaa aaagccctcg ccgcgcaaaa
     6361 catcgccgtg cacgtcgttt ccatgccgtc caccaacgta ttcgaccgcc aagacaccgc
     6421 ctatcaagcc gccgtcctgc ccgaaagcct gccgcgcatc gccgtagaag ccggacacgc
     6481 cgacggctgg tacaaatacg tcggactgaa cggcgcagtc gtcggcatca accgcttcgg
     6541 cgaatccgcc cctgccgatt tactcttcaa agcattcggc tttaccgtgg acaatgtggt
     6601 cgatacagtg aaatcagtgc tgtaacccca ctcctaaaca aatgccgtct gaacgctttt
     6661 cagacggcat ttttttgccc tcccgccttg atgcttcctc attccaccaa tctccattat
     6721 aatatttgcg aatcactctt attcacattt caaaaggaga aacgtatgaa cactcgcacc
     6781 gaacacgaca ccatgggcaa tgtcgaagtc ctgtccgaag cctattgggg cgcgcagacc
     6841 cagcgcagcc gcaacaattt caaaatcggc ggcgaaaccc tgccgcagcc gctgatttat
     6901 gctttggcgt tggtgaaaaa agccgctgcc gcgaccaatg tttccctcgg caggattaag
     6961 ccggaacagg cggatttgat tacgcaggcg gcggatgatg tgttgaacgg caagctcgac
     7021 ggacagtttc ctttagtggt ttggcagacc ggctccggca cgcagtccaa tatgaacatg
     7081 aacgaagttc tggcgaaccg cgccaacgaa atcgccggta cgggtttggc ggcgtatcag
     7141 cccgtccatc ccaacgacca tgtgaaccac gcgcaatcga ccaacgacgc attcccgacc
     7201 gctatccacg ttgccgccgc gattgaaatc aaccgccacc tcatccccgc cgtaaaagcc
     7261 ctgcgcgaca ccttggacaa aaaagcccaa gccttcgccc ctatcgtcaa aatcggccgc
     7321 acccacttgc aggacgcgac gccgctgact ttaggccaag aattttccgg ctacgtttcc
     7381 cagctcgacc acggcttagg ccgtctgaac gacgcgctta aagacttgta tgaacttgct
     7441 ttgggcggta cggcggtcgg cacgggtttg aacagccatc ccgaatacgc cgaaaaagcc
     7501 gccgccaaac tcgccgaatt gtccggcttg ccgtttgtca gcgcgccgaa caaatttgaa
     7561 gccttgggcg gacgcgatgc cgccgttgcc gcttcgggcg cattgaaaac gctggcggca
     7621 agcctgaata aaatcgccaa cgacatccgc tggctggcaa gcggcccgcg ctgcggtttg
     7681 ggcgaaatca aaatccccga aaacgagccg ggttcgtcca tcatgccggg caaagtcaac
     7741 ccgacccaat gcgaagcgat gaccatggtg tgctgccaag tgttcggcaa cgacgttacc
     7801 atcggtatgg cgggcgcgtc gggcaatttc gagctgaacg tctatatgcc cgtcatcgcc
     7861 tacaacctct tgcaatccat ccgcctgttg ggcgacgcgt gcaacagctt caacgaacac
     7921 tgcgccgtcg gcattgaacc cgtaccggaa aaaatcgact atttcctgca ccattccctg
     7981 atgctcgtta ccgcgttaaa ccgcaaaatc ggttacgaaa acgccgccaa agtcgccaaa
     8041 accgcctaca aaaacaacaa atcgttgcgc gaaaccgccg ttgagttggg cttgctgacg
     8101 ggcgaagaat ttgacgaact ggtcgttcct gccgatatgg ttcatccgcg ctaatctttc
     8161 cctcaaataa aatgccgtct gaaacctcgt tcggacggca ttttccgttg cctgcgaact
     8221 agcggcgttt gaacagcctg tcccccaccg ccgccgtaac cgcacccccg accacgacca
     8281 gtgcgccggc ataacccaaa ccgttcatat ccggcgcggc aaaagtatca ggcatcacat
     8341 aatgcccgag caaagaaaat attacggtaa acacggggag caaggttgtt accgcgctga
     8401 ctttggaagc ctcccaatgt ttcaacgcct cgccgaacga gccgtaaccg attaacgtat
     8461 tcaagcagca atacgcaaaa caaacccacg ccaacgtacc gtccaaactt ccgatgtgtg
     8521 ccagttcggc aaacggcagg aacacggcgg cacttgccgc ataaatcaac agcagaatct
     8581 gttgcggccc gaattgcgcc gacagcagct tttgcgccac ggcataacac acccatgcca
     8641 tactgcctac cgcacacagc aacacgccct tcgcatacgc gcccaaaccc gacaactcgc
     8701 cgaatttatc gttaaaaaac ataagcaaac cggcaagcag caaaaccaag ccgattttct
     8761 gagcggcagt catccggtct ttaaacacca acacaccgac aacaatcatc gtaaacggcg
     8821 aaatctgcca caaaacctgc gtcgtggtcg gcgaaatata atgcagccct tgggcaatca
     8881 gcacaaagtt tgccgaaatg cccgccacgc cgagcagcag cagcctgaat gagcaccaag
     8941 aaaaatcccg ccacttcggc agccgcccgc ccaatgccag caaaacaaac aataccgccg
     9001 ccgccacggt aaaacgcacc cacaccagcg tcggcgcatc gacaaacttc aatacctgcc
     9061 gcacggcaat cggcagcgtt ccccacgtca tcgccgccaa aagtgccaac gcgaagccta
     9121 ggagcggcct ttggttttcc atcctgattt tcctattttt aaacaaccgt attgccggac
     9181 gatgccggtt tgccgcatcg ggcaatgatg gttcaagcgt ttgacgtttg attccaaccc
     9241 tttgatttca aacaaaccgg ctgaagcccg actattgctt cgcgctattt ggaaacaccg
     9301 cctgaatttt aaaatgccgt ctgaaaacag cggggacgtg cggcaggcat cactgcctca
     9361 aaacgcccga acggcgcaat cgcaacgttc cgcccaacgc aaggggcggc aacaagccgg
     9421 ctcggatgca aaaaagagaa accctgcccc gtaaggttta aggtttctcc gtcctttatg
     9481 atttccctct gcgaggatgt ccggccgtaa aattcagaac gggatgtcgt cgtcaatatc
     9541 ctcgaccggg gcggcggcag gcacgggttg gcggcgcggc gcggctggtg cttcttgggg
     9601 atgggacggc gcgtcggagg cgggctgccg gctttgctgt gcggggcgtt ggtaagcctc
     9661 ctgactctga ccgtaacctt cctcgtaagg cgcaccgccg ctgttttcat tgcgcccgcc
     9721 caacattttc atttcgttgg cgacaatatc gtaagcggtg cgttcgatgc cgtctttgcc
     9781 ttggtatttg cggctttgga ttctgccttc cagataaacc agcccgcctt ttttgaggta
     9841 ttgcccggca atttccgcca gtttgcggta catcgtgatg ttgtgccact cagtacgctc
     9901 tacacgttgg ccgttgcggt cgttccaagt ttcgctggtg gcgacgctga aattacacac
     9961 cgcctcgccg ttgggcatat agcgcacttc gggatcgcgt ccgaggcggc cgataaggat
    10021 aactttgttc aatgacattt tttaaactcc tgtgatgatt ttttcagcgg cagcctgatc
    10081 gaaacccttc tgcaacactt tgagatagac ggtctgcccg tcgaaactga aaccgatgtc
    10141 ttccacaccc tcaagctccg acaaggcgcg gtataacact tcctgattga cctgccacac
    10201 gccgccgaca gggtaactga ggtttttgac gggcttgggc gcaggcgata aaacggcaat
    10261 caccagccac agcagcatca atatgctgca aaaggcaaac acgccggaaa agccgtattt
    10321 ttgaaacagc aaaccgcctg ccacgccgcc ggcaaacagt ccgagcgact gcatcgtgtt
    10381 gtacacgccc atcgccgtac ccttcaggtc ggacggcgcg attttggaaa ccatagacgg
    10441 caggctcgct tccaacacat taaaaccgat aaagtaaaca accaaataag cggtaatcaa
    10501 gcctaccgag cgcataccgg acagcaaacc gagctgtgcc gccgcgatgc aggcgatgcc
    10561 caagataaaa acctgtttga gcttgttgcg cgtctcgccg acgataatca gcggaaccat
    10621 caccaccaag cccgtaatgg tcgagggcag atagactttc caatgctgga ttttttccaa
    10681 accgagctgc gtcatcgcaa aaggcagcgc ggtaaacaat gccatttgcg cggcgtgcag
    10741 ggcgaaaatg ccgaaatcga gcgtcagcag cctacggttt ttcaaaactt cgcctatgcg
    10801 cgaaggctgc gcctgcgtat cttcgtgcag cttggaaacc tccgggtcgg gagtcatcca
    10861 cgccaccacg ccgatactgg caacggtcaa aataccggtc agcagaaaca agccgcgtac
    10921 gcccaccgcg tcggcaatca caggggcaac gacgaggctg accgaaaacg tcagaccgat
    10981 actcaaaccg atcatcgcca tcgcgcgggt acgtacgccg tcgcgcgtca aatccgccag
    11041 cagcgcggta accgccgcac tgaccgctcc cgcaccctgt atggcgcgtg cggcgaccag
    11101 catgggcagc gtatcggcgg cggcggcaag gaagctgccc gctgcaaaca caatcagccc
    11161 gacataaatg gttttcttgc gcccgaactt gtcggaagcg atgcccaaag gcagttgcag
    11221 cagagcctgt gtcagcccgt aaatgcccat tgccagcccg accagcgttt tgttgccttc
    11281 cgcgccgggc agcgaggcgg catacaccgc caatacgggc agcacgagga acatacccag
    11341 catacgcagc gcgtacacgc cggaaagcgt cgtactggcg cgccattcgt gcggaaacat
    11401 ttggatgcgg ttatctcttg ccatcatatt ttttcagacg gcatcaacag ttgcaatgcc
    11461 gtctgaactt ccagtgaaca gattttcgga ttatacagga ttcgccgtat ttcggttgcg
    11521 gcgcgggttc aaaatcaacg ccactgccag cggttgcgcc acgcgcccaa aacggcgttc
    11581 ggatatttat tgctgcccaa gctgccgtta aagcgggcaa gcgcgcggac gatgttgcct
    11641 ttttcaagat tccggtaatg gcgcaggatg gtacagccgt aacgcaggtt ggtgcggatg
    11701 tcgaacaggt tgtgcgccgg tttgccgatg tagtttttcc aaaacggcat aacctgcatc
    11761 aggccgcgcg cgccgacacc gctgattgca tactggcgga acgcgctttc cacctcaatc
    11821 agccccaaca caatctgcgt atccaaaccg gcccggctgc tttcgtactg gatattgacc
    11881 agcagcctgc gccgctcctc ctcctcgggg acgaaccttg ccaaacgtgc cgacatggca
    11941 gacaaccaac gttcgccctc tttcggattg tcaaacacca gcctcggcgg attgacgctg
    12001 ccgacagaac tcctcatcac ggaagccaca tcgtcggcaa gcgtttcctc acgttgcgcg
    12061 ccggcgtgcg ccagaggact gagcaacaac gcaccggcgg cacacaacag gcggcggcgt
    12121 tgcagattaa cgggtagggt atcggtcggt tttttcatag ggaacggggg cgcgtccgga
    12181 cgtttcagac ggcattaaat attcaaacag acaatgattg cttccaacgc gaaaaaccgc
    12241 gcgcaaaatc caagcgcggc atatcgccct gcccttttcg ggcaaacctc aattctaccg
    12301 ccctcaagaa cgcttgtcca aacaggcaca ggcaacaccg cccgggcatt tccgttttca
    12361 ccggttatcc gtcgtccgga ttatgcagca gcaccatcag cgcatcacgc ttttcgggcg
    12421 gcagcagg
//
LOCUS       DAWKOG010000047        12393 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.047, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000047 DAWKOG010000000
VERSION     DAWKOG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12393
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.047"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..309)
                     /locus_tag="WJK66_000971"
     CDS             complement(<1..309)
                     /locus_tag="WJK66_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEZ7578699.1"
                     /translation="MQTRILSAVLLAFSTAAFAGGAFTLQFDNPSEDGGFTQNQLLSA
                     PYGFGCSGGNASPALSWKNPPAGTKSFVLTVYDKDAPTGLGWMHRVVADIPADVHRRN
                     A"
     gene            620..1372
                     /locus_tag="WJK66_000972"
     CDS             620..1372
                     /locus_tag="WJK66_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEZ7578700.1"
                     /translation="MITLPSLNNLPSKWDEIRHCLETRVFECGVMPHPNLTECEAGQF
                     DRDFWDNIGCAPEEYVRIRRAIRLLEVRYPDSLNELVCAAIATPLGEMLAVFGNKGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSILIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            1563..1638
                     /locus_tag="WJK66_000973"
     tRNA            1563..1638
                     /locus_tag="WJK66_000973"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1596..1598,aa:Asn,seq:gtt)
     gene            1769..2779
                     /gene="waaF"
                     /locus_tag="WJK66_000974"
     CDS             1769..2779
                     /gene="waaF"
                     /locus_tag="WJK66_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEZ7578701.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAIIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSID
                     ERRREISVETFGLDIGKPVLAFCPGAEFGPAKRWPTRYFAELGKHYSAAGWQVWLFGS
                     QKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            2834..3280
                     /gene="smpB"
                     /locus_tag="WJK66_000975"
     CDS             2834..3280
                     /gene="smpB"
                     /locus_tag="WJK66_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEZ7578702.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            3646..5682
                     /locus_tag="WJK66_000976"
     CDS             3646..5682
                     /locus_tag="WJK66_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEZ7578703.1"
                     /translation="MYYETRSKQASKQKFKKSFIISLFFSILYTSPLLAVDYVYDNSK
                     LTNEQIDRLKKLRDKSSEYWKQETYLLKPTNGSSQNSLKVPALYSKTHQFENINNSKK
                     ISFYDKEYTEDYLVGFARGFGVEKRNGEEEKTLQQYFKDCVNTGNSNNDQCEQPSFGN
                     SGPRLIKSDIFALAPQIKNSHINSEILSVGNYIEWLRPTLNKLAGWEEHLYSGLDPFH
                     YITVTDNSHVIGQTISLDQFILENSLWEPRWDSDVGELKTTNADIRFNTKSESLLVKE
                     DYAGGARFRFAYDPKEAKNTALIFEKNVTGTSNIIFENPIDDLKSLDGHQIIKVNGTA
                     DKRAFRLSGKHQKGIYTLSLQQRPEGFFTKVQERDDIAIYAQQAQAANTLFNLRLNDK
                     NSDIFDRTLPRKGLWLRLISGHLSQDVQGKTAPVEGNRKGIQLGGDVFSLQNHNYQLS
                     VGLMGGQAEQRSTFRNPDTDNLTTGNVKGFGAGVYATWHQLQDKQTGAYADSWVQYQR
                     FRHRINTEDATERFTSKGITASIEAGYNALLAEHFTKKGNRVRFYLQPQAQLTYLGVN
                     GKFSDSENAHVNLLGSRQLQSRVGVQAKAQFSLYKNIAIEPFAAVNALYHNKPFGVEM
                     DGERRVINNKTAIESQLGVAVKIKSHLTLQATFNRQTGKHHQTKQGALNLQWTF"
     gene            complement(5863..7230)
                     /locus_tag="WJK66_000977"
     CDS             complement(5863..7230)
                     /locus_tag="WJK66_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ7578704.1"
                     /translation="MPDLHTEFSRLLPADEIAELSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGERVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKFPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWTLRENISASQRKLGTSIKHD
                     IAVPIGHVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPDILSNEAYRYED
                     DINSTVYRNVLACNGTVAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(7340..7636)
                     /locus_tag="WJK66_000978"
     CDS             complement(7340..7636)
                     /locus_tag="WJK66_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578705.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPATEAPAAEAPATEAPAA
                     EAPAAEAPAAEAPATEAPAAEAPAAEAAATEAPAAEAAATEAPAAEAPAAEAAK"
     gene            8109..8591
                     /locus_tag="WJK66_000979"
     CDS             8109..8591
                     /locus_tag="WJK66_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ7578706.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDSELVRIEDVGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            8678..9490
                     /gene="hpnC"
                     /locus_tag="WJK66_000980"
     CDS             8678..9490
                     /gene="hpnC"
                     /locus_tag="WJK66_000980"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEZ7578707.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLEGLRRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYGNFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEGQIAEGRADAAFQRLMAYECRCAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKRALWK"
     gene            complement(9561..10031)
                     /locus_tag="WJK66_000981"
     CDS             complement(9561..10031)
                     /locus_tag="WJK66_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEZ7578708.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSALEQKALRIVEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            10122..11963
                     /locus_tag="WJK66_000982"
     CDS             10122..11963
                     /locus_tag="WJK66_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEZ7578709.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPRAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFALGGILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
BASE COUNT         3063 a         2723 c         3412 g         3195 t
ORIGIN      
        1 cgcgttgcgg cggtggacat cggcgggaat gtcggcgacc acccggtgca tccagcccag
       61 tccggtcggc gcgtctttat cgtaaacggt caggacgaaa ctttttgtcc cggcgggcgg
      121 atttttccac gacagcgcgg gcgaagcatt gccgcccgaa cagccaaatc cgtaaggcgc
      181 gctcaaaagc tggttttgcg tgaagccgcc gtcttcggac gggttgtcga attgcagcgt
      241 gaatgcgccc ccggcaaagg cagcggttga aaaagccagc agtacggcgg agaggatgcg
      301 ggtttgcata gatgattcct ttgcttgatg aataagtgga ttcattatag gctgtcaacg
      361 gcggcaggaa tgtgccgaaa cggttggatg atgtgccaga atgcgcaaat gcaaaaatcg
      421 tggtttcaga cgagcctttg caggtcgtct gaagtcttta attcctttcc ctgaggcttt
      481 ggaaatatcg catatttagg cattgccgca acccgaccct caagatttac gtcatagccc
      541 gttcctgcat cggcgtgcgg gaatgacgag aattggggat tttgccgcca aatccatgat
      601 agagatacgg agcaaacgaa tgattaccct gccttcctta aacaatctgc cgtcaaaatg
      661 ggatgaaatc cgccattgcc tcgaaacccg ggtttttgag tgcggcgtta tgccgcatcc
      721 caatctgacg gaatgcgaag ccgggcagtt cgatcgggat ttttgggaca atatcggctg
      781 cgcgcctgaa gaatatgtcc gtatccgccg agccatccgt ctgttggaag tgcgttatcc
      841 cgacagcttg aacgagctgg tgtgtgccgc cattgccacg cctttgggcg agatgctggc
      901 ggtgttcgga aataagggat tgtgtctgct ggagtttgtc ggacagaagc atttggaaca
      961 ggaaattgcc gccgtccaaa aagccttgcg cggacggttt gtgtttcggg aggatgagcg
     1021 gacgcaactt ttgcggcagg aattggactt atacttcaag ggtcgtctga aaacctttgc
     1081 cacgcctttg gagcagattg gcaccgagtt tcaaaagcag gcatgggacg cgctcttggc
     1141 gattccttac ggcgaaacgc gcagctacaa ggagcaggcg cagcgtttgg gcaaccccaa
     1201 agccgtccgc gccgttgccg ccgccaacgg gcagaacaaa gtatctatcc tgattccctg
     1261 ccaccgcgtc atcggcagcg acggcaaact gaccggctac gcgggcgggt tgaaccgcaa
     1321 acagttttta ctggctttgg aacgcggcga agttcagacg gcattgtttt aagcggggga
     1381 gggcagaacg gctcatcggg cttaaatatg gtgtgatgac ggctgtggaa attcatacaa
     1441 cggcaatcct tttaaggcat cggtcttaat gtctttctga ttcatatgct tgtcgttttg
     1501 tcgcccattt ttccaaaatt aaggattgac acattttttc cggacgctat aatacgcccc
     1561 attccccgat agctcagtcg gtagagcgac ggactgttaa tccgcaggtc cctggttcga
     1621 gcccaggtcg gggagccaga attttgaagc ccgaacttga atgttcgggc tttttcatat
     1681 ttgcacgtac ggcgttcgga atgagacaag ataggttttc caacggtttc gcggtaaaat
     1741 tccgcctgtc tgatttcggt ttcttgttat gtccatcaaa atcctgatta tttcccccag
     1801 ttggataggc gactgcgtga tgacccagcc cttgttccgc cgtttgaagg aacttcaccc
     1861 cggttgcacg attgatgtgt tcgcaccgaa gtggtcgatg gcggtgtttg agcgtatgcc
     1921 ggaagtgaat gagattcttg aaaatccgtt cggacacggt gcgttggagt tgaaacgccg
     1981 ttggcgggtc ggtagggatt tggggcggcg cggatacgat caggttatcg tgttgcccgg
     2041 ttctttgaaa tcggcaatca tcgcgctggc gacaggtatc ggtaaaagga cgggttatgt
     2101 cggtgaaagc cgttattttc tgttgaacga tatacgcagg ctggataagg aacgtctgcc
     2161 tttgatggtg gacagatata cggctctcgc gcatccgagc caggaggatt ttgacgggca
     2221 ttcgggattc cccgagtttt ccattgatga acggcggcgg gaaatttctg tcgaaacctt
     2281 tggcttggat attggaaagc ctgttctggc tttttgtccg ggtgcggaat tcgggccggc
     2341 aaagcgttgg ccgacaaggt attttgccga gttgggcaaa cattattcgg cggcgggttg
     2401 gcaggtttgg ctgttcggtt cgcaaaaaga tgatgaaatt gccgaggaaa tcaactgcct
     2461 ttcagacggt atgtgtgtca atttgtgcgg caaaaccgat ttgtcgcagg caatggattt
     2521 gctgtcgttg gcggacacgg tcgtgtgcaa cgacagcggg ctgatgcact tggcggcggc
     2581 attgggcagg aaggtggtgg cggtttacgg ttcttccagc ccgacgcata cgccgccttt
     2641 gagcgacagg gcgaaaatcg tcagcctaca cttggaatgt tcgccctgtt tcaaacgtga
     2701 atgtccgttg gggcataccg actgcctcaa caggctgtat cccgagaaga ttgtgcaggc
     2761 ggttgaagag gcggtatgat gggttctgtt ttcgggttta tgccgtctga cggcggatat
     2821 tgataggatg tttatggcta ttgcgaacaa taaaaaagca tttcacgatt ttttcattga
     2881 agaccggatt gaagccggtt tggtcttgga aggctgggaa gtcaaagcaa tccgcgccgc
     2941 gcgcgtacag cttaaagaga gttatatcta ttggaaaaaa gacgcgtttt atctggtcgg
     3001 ctgccatatt acggctttgc ccacagcctc gacacacatt aaaccagatg ccgtacgtcc
     3061 gcgcaagctc ttgttgaacc agtcggaaat caacaaatta atcggcaaaa ccgaacgtgc
     3121 cggctacacc atcgtgccgc tggatttgca tttttcgcgc ggaaaaatca agatggaaat
     3181 cggtttggca aaaggtaaga aacagcacga caaacgtcag agtatgaaag aggcggattg
     3241 gaaacgcgag aaacagcggt tgattaagca tacgcgctga ttgtggaaac agtgcttcag
     3301 acggtatggg caaggctgcc gcctgatgtg taaacgttct acacctaaac cagcatccgc
     3361 tttaatcagt acggcatgat gccccaaacc ttcgtcaaag gtttgagcaa tttgatgata
     3421 aattggcatt agccaagggt aaagtgcggt catttttcct gaaatttttg taaataacga
     3481 gggcaattat acacattttc aaactggctt gacaaaatag caaaaagcaa acgtttactt
     3541 ttaataaata agaaataaat agagaaaaat gtgagctaaa tgacattttt tgttcaattc
     3601 tatgctaaaa tccacgcccc atttattttt acttgacaaa ggaaaattta ttatgaaact
     3661 cgaagcaagc aagcaagcaa gcagaagttt aaaaaatcat ttattataag tctatttttt
     3721 tctattcttt atacctctcc gcttttggct gttgattacg tttacgacaa ttctaaatta
     3781 acaaatgagc aaattgatcg cttaaaaaaa cttagagata aaagcagcga atactggaaa
     3841 caagaaactt atcttctaaa accgacgaat ggtagctctc agaactctct caaagttccc
     3901 gcactatact ctaaaacaca ccaattcgaa aacatcaaca actcaaaaaa gatttctttt
     3961 tatgacaagg aatacactga agattacctt gttggcttcg cccgaggttt cggggttgaa
     4021 aaaagaaatg gggaagaaga aaaaacactc cagcaatatt ttaaggactg tgtaaatact
     4081 gggaattcca ataatgatca gtgtgaacaa ccatccttcg gaaactctgg cccaagatta
     4141 ataaagagcg atattttcgc cttagctcca caaataaaaa atagccatat caacagcgaa
     4201 attttatctg taggtaatta tatcgaatgg cttcgcccaa cacttaataa gcttgctggt
     4261 tgggaagagc atctatattc tggtttagat ccattccact atattacggt tacagacaat
     4321 tcacacgtta tcggacaaac aattagtttg gatcaattca tattagaaaa ttctctctgg
     4381 gaaccccgtt gggattctga tgttggggaa ttaaaaacaa cgaatgcaga catccgtttt
     4441 aatactaaaa gtgaaagttt actcgtcaaa gaagattatg ctggcggagc tcgttttcgt
     4501 tttgcttacg atcctaaaga agcgaaaaat actgcattaa tttttgagaa aaatgttact
     4561 ggcacttcca acatcatctt cgaaaatcca atagacgatt taaaaagcct cgacgggcat
     4621 caaattatca aagtaaatgg cacagcagat aaacgcgcat tccgtctttc tggcaaacac
     4681 caaaagggaa tttatacact ctctttacaa caacgcccag agggcttttt taccaaagtg
     4741 caagaacgcg atgatattgc gatttatgca caacaggctc aagctgccaa tactttattc
     4801 aacctacgtt tgaacgacaa aaacagcgat atttttgacc gcactttacc acgcaaaggc
     4861 ttgtggttgc gtttaattag tggtcactta agccaagatg tacaaggtaa aaccgcacca
     4921 gtagaaggaa atcgtaaagg catacaactt ggtggcgatg ttttctcatt gcaaaatcac
     4981 aactatcaac tttccgttgg cttaatgggc ggacaagcag aacaacgcag tactttccgc
     5041 aacccagata cagacaatct tacaacggga aatgtgaaag gctttggtgc aggcgtttac
     5101 gccacttggc atcagcttca ggacaaacag acaggtgcgt atgcggatag ctgggtacaa
     5161 tatcaacgct tccgccaccg cattaacact gaagatgcta cagaacgttt tacttcaaaa
     5221 ggtattaccg cctcaattga agcgggttac aatgctttat tggcggaaca cttcactaaa
     5281 aagggcaacc gtgttcgttt ttacctacaa ccacaggcgc aattgactta tttgggggta
     5341 aacggaaaat tcagcgatag cgaaaatgcc cacgtgaatt tacttggctc tcgccaatta
     5401 caaagccgag tgggcgttca agctaaagct caattttcac tctataaaaa tatcgctatt
     5461 gaacctttcg ccgctgtgaa cgcgctttac cacaacaaac ctttcggtgt ggaaatggac
     5521 ggagagcgtc gagtgataaa caataagacg gcaattgaga gccaattagg tgtagcagtg
     5581 aaaatcaaat ctcacttaac cttacaagcc acattcaacc gccaaacagg caaacatcat
     5641 caaactaaac aaggtgcgtt gaatttgcag tggacgtttt aagttaagtt gtaacaaaat
     5701 aaaatcggca gggaaattca ctgccgattt ttttatttta aaaatgcggt catttttcgt
     5761 gtcatttcca ccaatttttt accgcactca cgagctcgcg ctaaaccgac aatcggatct
     5821 attgtccctt tctttgttgt gtatgcaaat cagaaatgcc ggttacggaa gcagtttgcc
     5881 cggattcata atgttatatg gatcaaggtg ttgtttgatg cttttcatca gggcgatttc
     5941 ggcaggcgtg cgtactttgt ccagccactg ttttttgatg atgcctatgc cgtgttcggc
     6001 ggcaactgta ccgttgcagg caagaacgtt gcgatagact gtgctgttga tgtcatcttc
     6061 gtaacgatat gcctcgttac tgagtatatc cggcagaaaa gtattgtagt gtaggctgcc
     6121 gtcgcccaga tgtccgaagc agacgatttg tatgcctttg aaattctgtt ccaaatcttt
     6181 ggcacaccgg cggacaaagt cggcaacgtg cccgatgggg acggcaatat cgtgtttgat
     6241 gctggtgccc agtttgcgtt gcgatgcgga gatgttttca cgcagtgtcc acatatggat
     6301 acgttcttgt tcgctttgtg ccaatacgct gtcggtaaat ccttttttat aaagaaattc
     6361 gacaagccga tcatcaagat tgctgtcggg taatgagtcg gtcaactcaa gtaaaatatg
     6421 ccattctgaa tgtgtcggca gggggaattt gctgaattcg gaagacaact cggcggcaaa
     6481 acggccgatc agctcaaaac tgcataggcg ttcggcaaag tgagcttggg tttcggtcag
     6541 caggcggacg gcggattcga tgtcgggtat gccgacccat gccgttgcct tgtccgaggg
     6601 acgggcaaaa agcttgagtg tggcggcagt gatgatgccc agtgtgcctt cgctgccgat
     6661 aaacagatgg cgcaggtcgt agccggtggt gtttttatgc aggggatgga gatgggaaac
     6721 ccgttcgccg ttggggagga cgacttccaa accgataacc aggtcgcgca tcgtgccgta
     6781 acgcaatacg ttcaaacctc cggcattgca ggcgatgttg ccgccgattt ggcacgagcc
     6841 ttcgctggcg agactgagtg gaaacagcct gtttgaggct tcggctgcct gttggacggt
     6901 ttggagtacg gaacctgctt cgacggttat gcagttgtct gacaaattga tgctgcggat
     6961 gcggttgagt ttggaaaggt tcagcaatac gccgttttcc gatactgccg cgccgcacaa
     7021 accggtattg ccgccttgcg gcgtaaccgg aatacggtgt tcgtaacaaa aacgcataat
     7081 ggtttgcacg ctttcaacgc tgcgcggctg caaaatgatg tctggtgcag acgtaaagcg
     7141 gttgcgctgg tcttttaaaa gcgtcggaga aagttcggca atttcatctg ccggcaggag
     7201 tcgggaaaat tcggtatgaa ggtcaggcat gtttgtcgga agggtaggga gaagattgag
     7261 gaagggaatc tgatgccgtc tgaaaaatca aaaaagcagg atactgaacg tatcctgctt
     7321 ttttgcaagc ggaaaatgct tattttgcag cttcggcagc aggagcttca gcggcaggtg
     7381 cttcggtagc ggcagcttca gcggcaggtg cttcggtagc ggcagcttca gcagcgggag
     7441 cttcggcggc aggtgcttca gtagcaggag cttcggcggc aggtgcttca gcagcgggag
     7501 cttcggcggc aggtgcttca gtagcgggag cttcggcggc aggtgcttcg gtagcgggag
     7561 cttcagcggc tttttcaccg ccgcaggctg ccaggaccaa agacaacaaa gcagcggcaa
     7621 acagggattt tttcatttta gtaaccttta gaaccaattt aaattattga aacaaatagg
     7681 tctttatcga ccatactaca aaccgggttg gaaattcatc gctgaatttg cgtaattatg
     7741 cactatggaa taagggattg caatgtcgta cagatgggaa aaaggtttga atgaaaagtt
     7801 ttctggcggg cggattgata aatcctgtaa aacggttttg ctgttaaaaa ttcgataggg
     7861 ataaatatta tagagattaa tataatttca gtatcttgta tgatttatta aggcggattg
     7921 ggaaagtcgg cagacaggtc ggaatggttt taataatgtt gtaaaaaagt tacatcgcaa
     7981 taaaataaca aaaatttaca ttgaaatatg tgctgtacag tttatgaaca ggttaacggt
     8041 aatcggccgg cgcggcggtt gtgttagaat gcgcctgtaa tcaatctgct gttcctgaaa
     8101 ggacaaatat ggctatcgtg aagaattccg tggtttcgct gcattatgag atgtatgatg
     8161 ccaacaatca gcttttggac aaaaccgaag aaccgattgc gtatctgcac ggcggttacg
     8221 acggcatttt ccctttggtg gaagaggcgt tgcacggtaa ggatgccggc gatacggtcg
     8281 atgtggcgct ttcgcccgac gatgcgttcg gcgagcagga ttcggagttg gttcgtattg
     8341 aagatgtggg cgtgttccct gttgaagtcg aagtcggcat gatgtttgaa gccgacgatc
     8401 ctgaaaccgg cgatgttgtc gtctatcgtg taaccgatgt tgccgacggc aaggcggtgg
     8461 tggacggcaa ccatcctttg gcaggcatga aaatccgttt taaggctacg gttgaaagcg
     8521 tgcgcgatgc atccgatgag gaaatcgcac acggtcatgt ccacggcccg cacggtcatc
     8581 accaccactg attgccggca gcttggaggt atctgatgcc gtctgaaaag ggtttgttcc
     8641 gtttcagacg gcattttgtt tgatggatgg agaaggtatg tcggttgggc attatgaaaa
     8701 ttttcccgtc ggttcgctga ttttgccgcg caggttgagg aagccggttc atgcggtgta
     8761 tgcgtttgca cggacggcgg acgatatggc ggacgagggc agtatgccgt ctgaagccag
     8821 gttggcgggg ttggagggtt tgcggcgcga gttggacgtg ttggcatcgg gcggccggtc
     8881 ggcgcatcct ttgattgcac gattggatgc cgaggcggtt gtgccgttcg gtttggattt
     8941 gcagccgttt tatgatttgc tttctgcatt ttctcaggat gtggttaaaa caaggtatgg
     9001 aaatttcggc gagctggccg attattgccg acgttctgcc aatcctgtcg gacgcattat
     9061 gctggctttg tacgggaaaa cggatgcggt gtgcgtggcg caaagcgacg gcatctgtac
     9121 ggctttgcaa ctggtgaatt tttggcagga tgtggctgtg gattggcaaa agggcagggt
     9181 ttatattccg caggacgatt tgttgaaatt cggtgtttcc gaaggacaga ttgcggaggg
     9241 tagggcggat gcggcgtttc agcggctgat ggcgtatgag tgccggtgcg catttcgtat
     9301 gctgaaggcg ggttcgcctt tagcgcgcga attgaaaggg cgtatcggtt tggaactccg
     9361 tatgattgtg ttgggggcgc agttgatttt gcagaaactg gacgcgtgcc gatacgatgt
     9421 gtttgcacaa cgccccgttt tggataagaa ggattggttg attatgctga aacgcgcgtt
     9481 gtggaaatga tgggggaaat gcatgccgtc tgaaaggggt tgttccgctt cagacggcat
     9541 tttgcaggcg gttggtatgg tcaggcgatt tcatgtgggt tcaacctggc ttgatcttct
     9601 gcacccaagc cgatgagttt gcgcagtcgg gtcatgtaga aattgacatc caaaccggtg
     9661 ccgtagcgtt gcgccgtcca cagtgtttcc gccagtgcct ccatcatttc gtgttcggct
     9721 tccagccagc cgcgtttggc gcacagggtg tcgtggattg cgcgtatgcc gtgcggctgg
     9781 tctatgcccg cctgttcttg gacggacaga tgcagcgaca tatgcaggaa ggggttgctt
     9841 tcgccgtttt cgggcagcca gtcggtgtcc agatggtctt cgatgcgttc gagatagcgg
     9901 tggtattcgg gatgggcttc gacaatgcgg agggctttct gttccagtgc gctcagttgc
     9961 agcggattga gccgctgctg ccacacgcgg gcgaaaaagc ggcggacatc gtgggtattg
    10021 acatcgtaca tggcgggttc gataagatgt ttatggaaat aggttcggat aatggcgggt
    10081 tttacataaa ttaaatccca ccgtcaacct taccatttat aatgcagaca aattatttgt
    10141 ctgaaacgga ttcgattatg aaaaaactga tttgcctgtt cgccgtattt ttgatgttgt
    10201 gcggacgagc tttcgcgctg gatgcgaacg atctgctgcc gccggaaaag gcattcgtgc
    10261 cggagcttgc cgttgccgac gacggtgtga acgtccgttt caggattgcc gacggatact
    10321 atatgtatca ggcgaaaatc gtcggcaaga ccgatccggc ggatttgttg ggacagcctt
    10381 ctttcagcaa gggcgaagag aaggaagacg agtttttcgg caggcagacg gtttaccatc
    10441 acgaagcgca ggttgccttt ccttatgcaa aggctgtcgg cgaaccgtat aaattggttt
    10501 tgacctatca gggctgtgcc gaagccggcg tgtgctatcc gcccgtggat accgagtttg
    10561 atatttccgg caacggcact taccatccgc aaaccgacga accggcatcc gccaaagacc
    10621 gctttttgca gccttcctct caaaacggca gcggggcgct gccgcccccg aagggggatg
    10681 agggcggcga cagccgtttc aagctgtctt gggatacgct caacgccaat cttttggcgt
    10741 tttttctcgc cggtttgggt ctgagtttta ccgcctgtat gtatcccctg ttgccgattg
    10801 tttccagtat tgtggtcggc gacaaaaagg cgggcaaggc gcgggcgttt gtgctgtccg
    10861 tcgtttatgt tcagggtttg gctctgactt atacgctggt cggcattgtt gccggactga
    10921 cgggcgcgct gctgaccgta tggttgcagc aggcttgggt cgtgttggcg gcatcggctt
    10981 tgatggtcgt cttggcactg tctatgttcg ggctgttcaa catccagctt cccaacgccg
    11041 tgcagtcgta tttccaaaac cagagcagca ggctttcagg cggtaaaatc gtttccgtct
    11101 ttattatggg catattgtcc gcgctgattg tcgggccgtg cgtcgccccg ccgctggcat
    11161 ttgctttggg ctacatcggt cagacgggcg atgcggtttt aggcggtttg gcactttaca
    11221 ctttggcgtt gggcaccggc gttccgctga ttgccatcgg cacgttcggc gggcatatcc
    11281 tgcctagggc aggcgattgg atgaatgccg tcaaatacgc tttcggcttt atcctgcttg
    11341 ccgtcgccgt ttacctcgcc acgccgcact tgccctatta tctcgtcgtc gcgctgtaca
    11401 cgctgctgat gctggttcct gcctgtatgc tgctggtcaa cggacgcagg cagaaacgcc
    11461 gtccgaaagc tgtggcattc gcattgggcg gcatattgct gataggcggc gcgtggttcg
    11521 gctggcaggg cgcaaacggc aaaacgaccg cgctgcacca tttcctgacc ctcaatccgc
    11581 cggccgaagc aggcaaatct tcggaacacg gaaaaatgtt tgccgatact gccgcgctga
    11641 aggcagcgat ggatacggcg ttgaaagaac atcccgacaa acccgtcgtt ttggattttt
    11701 atgccgactg gtgcatttcc tgcaaagaaa tggcggctta cacgctcaat cagccggaag
    11761 tgcatcaggc agtcgatatg gaacgctttt tccaaatcga cgtaaccgcc aacacgcccg
    11821 aacatcaggc gttgttgaaa gaatacggtc tgttcggacc gccgggcgtg tttgtcgtcc
    11881 gctccgacgg cagccgcagc gagccgctgc tgggttttgt caaagcagac aagtttatcg
    11941 agtggtatga acaaaaccgc tgattccgat gtttcaaatg ccgtctgaag ttctcagacg
    12001 gcatttttgt cagaaaggaa aacggcaata tccactatac acacggaagc agtccaaaga
    12061 tgtgaaaacc gtgtgtataa gttggtttgc gtattgaaat taaaagataa aaatacgatg
    12121 attaaaaaat aggcataaga aaagataaac acagccggtg tttgccggtg tgtgtatctt
    12181 ttgtggaaaa acatcttaat gccatgtatt ttatggaaaa taataacatg attaaaaaat
    12241 agtcaatcat taaggttcag gtgggcggct gcaaccgtca atgtctgaaa agaggcagtg
    12301 ctgtttaagt atggaaagtc ggatatagtg gattaacaaa aatcaggaca aggcgacgaa
    12361 gccgcagaca gtacaaatag tacggaaccg att
//
LOCUS       DAWKOG010000048        11969 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.048, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000048 DAWKOG010000000
VERSION     DAWKOG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11969
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.048"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            141..575
                     /locus_tag="WJK66_000983"
     CDS             141..575
                     /locus_tag="WJK66_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEZ7578710.1"
                     /translation="MNFALSVVMLTLASFLPVPPSGAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNDVNKYCR"
     gene            complement(624..1967)
                     /locus_tag="WJK66_000984"
     CDS             complement(624..1967)
                     /locus_tag="WJK66_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEZ7578711.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLASDGIPLAGVWLDEAGTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIALANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(1972..2625)
                     /locus_tag="WJK66_000985"
     CDS             complement(1972..2625)
                     /locus_tag="WJK66_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEZ7578712.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYGDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            2776..3066
                     /gene="gatC"
                     /locus_tag="WJK66_000986"
     CDS             2776..3066
                     /gene="gatC"
                     /locus_tag="WJK66_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEZ7578713.1"
                     /translation="MALTLADVDKIARLSRLHLTAAEKEKSLQELNDIFAMVEQMQNI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAVAPEVRDRLYIVPQVIEE"
     gene            3129..4574
                     /gene="gatA"
                     /locus_tag="WJK66_000987"
     CDS             3129..4574
                     /gene="gatA"
                     /locus_tag="WJK66_000987"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEZ7578714.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNLEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTA
                     LQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRTAQFG
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPSAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPIGV
                     QLIGNYFSEAKILGAAHQIQLNSDWHGKRPE"
     gene            4571..5503
                     /locus_tag="WJK66_000988"
     CDS             4571..5503
                     /locus_tag="WJK66_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HEZ7578715.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLTDIESKKRSMYRKRYE
                     MLDNMVAEMKDGLKTEARGISDGIQTEKPLRRTA"
     gene            5546..6976
                     /gene="gatB"
                     /locus_tag="WJK66_000989"
     CDS             5546..6976
                     /gene="gatB"
                     /locus_tag="WJK66_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEZ7578716.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHAGVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLNICDGNMAEGSFRVDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLEQAINYEAEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAHDYRYFPDPD
                     LLPVIISDDRLQKAKAEMPELPKEMAARFVADYGVSDYDARLLTASRVQAAYFEEAAK
                     ESGQGKLTANWMNGELAATLNKEGMELADSPITAPRLAALVGKIADGTLSSKLAKKAF
                     EAMWAEPEASIAEIIEKHGLQQMTDTGAIEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
     gene            7068..8078
                     /locus_tag="WJK66_000990"
     CDS             7068..8078
                     /locus_tag="WJK66_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEZ7578717.1"
                     /translation="MNHTITLPDQTTFAAGDGETVLAAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQMGGHSEQALSEAEKAQGKILMCCTTAQSDISINIPGYKADALPVRTLP
                     ARIENIIFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANPPDQEGILE
                     LHIRRRENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQGSSRVVHFYWGARHQDDLYALEEAQGLADRLKNACFTPVLSRPRECW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(8208..8840)
                     /gene="pdxH"
                     /locus_tag="WJK66_000991"
     CDS             complement(8208..8840)
                     /gene="pdxH"
                     /locus_tag="WJK66_000991"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEZ7578718.1"
                     /translation="MDLHNIREDYSKRELSEGDCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            9061..10110
                     /locus_tag="WJK66_000992"
     CDS             9061..10110
                     /locus_tag="WJK66_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ7578719.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKDETDKTASQPY
                     GQKASDGIKPQNLPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANILK
                     WAEMLLPGEYRRKKA"
     gene            complement(10179..11720)
                     /locus_tag="WJK66_000993"
     CDS             complement(10179..11720)
                     /locus_tag="WJK66_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEZ7578720.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNTFSNAASVGVAETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSIGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMAGLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMIFVKA
                     LDLFS"
BASE COUNT         3071 a         3612 c         2972 g         2314 t
ORIGIN      
        1 tttgggaatc tgttttaccc caatatataa agcaccatat taaggcggag tgtcttcccc
       61 actttgaccc gaacccggaa aagacaccgc ccaagccaat cctgatgctg ccccgacagc
      121 caaccattaa ggaaatccta atgaactttg ctttatccgt cgttatgttg accctcgcct
      181 ctttcctgcc cgtcccgcct tccggagccg ccgtctttac ttggaaggac ggcggcggca
      241 acagctattc ggatgtaccg aaacagcttc atcccgacca aagccaaatc ttaaacctgc
      301 ggacgcgcca aaccaaaccg gcggtcaaac ccgcccaagc cgacgcaggg aagcgcacag
      361 acggcgcggc acaggaaaac aatcccgaca ctgccgagaa aaaccggcag cttgaggaag
      421 aaaagaaaag aattgccgaa accgaacggc agaacaaaga agaaaactgc cggatttcaa
      481 aaatgaacct gaaggcggtg ggaaactcaa atgcgaaaaa caaggatgat ttgattcgga
      541 aatacaataa cgatgtgaac aaatactgcc gttgagcagc ctcatcgaaa accccatgcc
      601 gtctgaaacg gcacggggtt tgtttatttc tgccagtagg ttttgacgtt gacgaactcg
      661 tacagcccga attcggacaa ttcgcgcccg taaccggaat ctttgactcc gccgaaaggc
      721 aggcgcaaat cgctgctggt atggcggttg ataaacaccg atcccgcctg tattttttcg
      781 gcaaaccgcc aagcgcgttc ggtatcggcg gtataaatgc aggcacctag cccgaacggg
      841 gaatcattgg caagggcgat ggcatgttct tcgttttcgg cgcgcaaaat cagggcggcc
      901 ggcccgaata cttcttctct ccagacgcgg caggcaggat ttaccctgtc taaaaccgtc
      961 gcgggataaa accagcctcg cccttgtggg atttttccgc cggtcaggca taccgcgccg
     1021 tttgaaacgg catcttcaac ctgcccgtga accctgtccc gcaaatcttc gcggtgcagc
     1081 ggtgcaagcg tagtatcggg atgtttgggg tcgcccattt tcaatttggc gcattcggca
     1141 agaaacagcg tgataaaacg atcggctgcg gcttcggtta cgatgatgcg tttggcggcg
     1201 ttgcacgatt gccccgcatc gcggaaacgg gaataacagg cttctgcggc ggcgcgttcc
     1261 aaatcggcat cgggcatcac gataaaggcg ttgctgccgc cgagttccaa gacggttttc
     1321 ttgaggtttg cgcccgcgtg tgccgcaagg atgcgccccg tatgcgtcga gccggtaaac
     1381 gccattgcat cggtatcttc aaccgccttg agcgtgcccg cctcatccag ccacacgcct
     1441 gccaaaggaa tgccgtctga agccaaatcg aacagtgcct gactgacgcg tgccacgctg
     1501 ggcgcgggtt tgacggcgca cgcgttgccc gcgcacatag cgggaacggc gaaacgcaat
     1561 acctgccaga cgggatagtt ccaaggcatg acggcaaaca ccacgcccaa aggctcgaag
     1621 cgcacctgac tcaaactcgc ctgcgtcgcg atggttttgt gggcaagcag ttcgggggca
     1681 aggcgggcgt aatagcgtat cagttcgata gacttgccga tttccgcacg gcattcgtgc
     1741 aagcagcgtc cgacttcctc acacaccatt tccgcaaaac gctctttctc cgcctccaaa
     1801 cggtcggcaa atttttgcag gcgcgcggca cgttcggtta cgcccagttg cgcgaacgcc
     1861 ccgccgcgca ttttcaaatc cgccagccgc cgttcaaact ccgcataatc ttgagctgga
     1921 cggcggtaaa gcgtttcgcc cgtaaataca ttgacactgt gaaacatcga atcaacctgc
     1981 cagttgcggg aatatcgttt tcagtcccga cacaataatc tccaccgata ccgccgccag
     2041 catcataccc ataatgcggt ttaaaatcgt cagccccgtc gcgcccagca ggcggctgac
     2101 cttcccggca acgattaaaa tggcataaca aatcgcactg accaccaaac cggccgcgat
     2161 aatcaacgcg atgtcgccgt atgttttagc cgccgaagcg taaataatca cggtcgaaat
     2221 accgcccggg ccgatggtga tcggtatggc gatgggcacg acggcaatcg ctccggcatt
     2281 gcgggcgggg cgcgcctgcc ccgtttccgg ctgcgcgccg agattctgct tggcgggatt
     2341 gtcgttgccg ttcatcatag aaatggcaat cagcaacacc aaaattccgc cgccgacctg
     2401 aaacgaaccg acgctgatgc ccaaaacctt cagcagcgta ccgccgatca gcgcaaatac
     2461 cgcaatcacg gcaaacacgg caacggcggc cgtccgcgcg accttcctgc gctccttcgt
     2521 gctgtgcccg ttggtcaggt caaggtaaag cgacaacgcg ctaaacggat tgatcagcac
     2581 caaaaaagcc acaatcagct tgccgatttc catgcccaat cccattattt ccccctcctt
     2641 caaacccgtg cggcaggcat ccgatgctgc aaattgccgc cgcaacggat ttttccgtta
     2701 taattaaaaa ttcaagcaat acgccccatc atacccgaac gacggtatct ttaccatcag
     2761 acaaggatgc ttttcatggc actgacactt gccgacgtag acaaaatcgc ccgactctcc
     2821 cgactgcacc tgactgcggc agaaaaagaa aaatcgcttc aagaattaaa cgacattttc
     2881 gctatggtcg aacagatgca gaacatcaac acagacggca tcgaaccgat ggcgcatccg
     2941 cacgaggccg ccctgcgcct gcgcgaagac gaagtaaccg aaaccgaccg cgccgccgaa
     3001 tatcaggcgg tcgcaccgga agtacgcgac cgcctgtaca tcgtaccgca agttatcgaa
     3061 gaataatccg aatacgcttc agacggcatc agcaataccg cccgaagccc tttaaggatg
     3121 gaagatttat gacccaatac acattgaaac aggcaagcgt cctgttgcag tccaaacaga
     3181 tttccgccgt cgaactggca agcgcatacc ttgccgccat cgccgaaaaa aatcccgccc
     3241 tcaacggcta tatcaccatc gaccaagata aaacccttgc agaagcccgt gccgccgacg
     3301 aacgtatcgc gcagggcaac gcctccgcgc ttaccggcgt acccgtcgcc tacaaggata
     3361 ttttctgcca aaccggctgg cgcagcgcgt gcgcttccaa aatgctcgac aacttcatct
     3421 ccccctacac cgccaccgtc gtccaaaacc tgctcgacga aggtatggta acgctcggcc
     3481 gcaccaatat ggatgagttc gctatgggtt cgaccaatga aaactcattc tacggtgcag
     3541 ccaaaaaccc atggaatctt gagcacgtac ccggcggttc gtcaggcggt tccgccgccg
     3601 tcgttgccgc gcgcctcgcc cctgccgcgc tcggttcgga caccggcggc tctatccgcc
     3661 aacccgcatc gcactgcggt atcaccggta tcaaacccac atacggcacg gtttcccgct
     3721 tcggtatggt cgcctacgcc tccagcttcg atcaagccgg cccgatggcg caaactgccg
     3781 aagactgcgc gattctgtta aacgcgatgg caggtttcga cccgaaagac tccaccagcc
     3841 tcgagcgcga aaaagaagac tacacccgcg atttgaacca accgctcaaa ggtttgaaaa
     3901 tcggcctgcc caaagaatat ttcggcgaag gcaacagcgc cgatgttctg actgcattgc
     3961 aaaacaccat tgatttgctg aaagcccaag gcgcggaatt gattgaagtt tccctgccgc
     4021 aaaccaagct gtccattccg gcctactatg tcctcgcctc cgcagaagcc agtaccaacc
     4081 tttcacgtta cgacggcgta cgttacggac accgtaccgc ccaattcggc gatttggaag
     4141 aaatgtacgg caaaacccgc gccgaaggtt ttggcagcga agtcaaacgc cgcatcatga
     4201 tcggcactta tgtactgtcg cacggctact acgatgccta ctatctcaaa gcccaaaaac
     4261 tgcgccgcct cgttgccgat gattttcaga cggcatttgc acggtgcgac ctcatcctcg
     4321 cgccgaccgc accctccgcc gcacccaaaa tcggagcgga tgcttcgccg gttgaaacct
     4381 acttgagcga catttacacc atcgccgtca acctcgccgg actgcccgca ttgaccctgc
     4441 ccgcaggttt cagcggcggc ggactgccca tcggtgtaca gcttatcggc aactatttct
     4501 ccgaagccaa aatcctcggt gcggcgcatc aaatccaact caacagcgat tggcacggca
     4561 aacgacccga atgaagcaga accgcacctt taccttcccc gattttcgca ccgtttacag
     4621 ctatgcgcct ttatatcggc tgcaacattt aaaatacaca ttgcgaaaat ttttcggaaa
     4681 aaaagaaatt tacgccttcg agcagtttgt caacgcctcc cctatccgtc aggggctgtt
     4741 cctccactgc ccgcaaaatg cctatccgct gctgcgcgaa tttgttgaca ggcgttttaa
     4801 ctgcaaacgc cgtttagatg cgatgacggc agattttctt atggcggaaa aactgttcgg
     4861 cacagacatt ctgcaccaaa tggaagacta ccgcttccat ttggtcttgg cgcacctttc
     4921 agacggcatc agcttgtggc tcaaccgcaa cgacaactgc gtcgaagaag gcgcgtggtc
     4981 tttatctttg cgcgacgaag caggcaaccg gctgtatatg gcgaccttcg cctttgtcgg
     5041 cacacacctg ctgacagcct ccgtacaagg gccggcgggt gaagaagcca aagacaccgt
     5101 ccgccgcata accaaacaac tccacggctt gcgtccccaa caactgatgg taaccgccct
     5161 gcaatatttc gccgccgtac tcggcttgga cggcgcaatg ggcattgcac aaaaacatca
     5221 ggtcaaactg cgctggaaac ttaaaaagcg tgtcaaaatg aattacgacg cattctggca
     5281 ggaatacggc gcaagtttgg aacgggacgg ctactggcat ctcccccaaa cccccgcccg
     5341 caaagacctt accgacatcg aaagcaaaaa acgctcgatg taccgcaaac gttatgaaat
     5401 gctggacaac atggttgcag agatgaagga cggtctgaaa acagaagcac gcggcatttc
     5461 agacggcatc caaacggaaa aaccgctccg ccggacagcc tgacgcgaag actatcgaat
     5521 tgatatttta gagaaagaag ctcttatgac ctgggaaacc gtaatcggct tggaaatcca
     5581 cgtccaattg aacaccaaat ccaaaatctt cagcggcgca tcgaccgcat tcggcgcaga
     5641 acccaacgcg cacgccggcg tggtggaatg tgctttgccg ggcgtactgc cggtaatgaa
     5701 ccgagaagtc gttgaaaaag ccatcaaatt gggtttggct ttggatgcga aaatcaaccg
     5761 gaaaaacgtg ttcgaccgta aaaactactt ctatcccgac ttaccaaaag gctatcaaat
     5821 cagccagttg gacttaccga ttgtcgaaca cggcaaattg gaaatcgtag tcggcgacga
     5881 tgtgaaaacc atcaacgtaa cccgcgccca tatggaagaa gacgcgggca agtccgtaca
     5941 tgaaggcttg aacggcgcga ccggtatcga tttgaaccgc gccggcacgc ctttattgga
     6001 agtcgtatcc gaacccgaaa tgcgctccgc cgccgaagcc gttgcctacg ccaaagcctt
     6061 gcacagcttg gtaacctggc tgaacatttg cgacggcaat atggcggaag gttcgttccg
     6121 cgtcgatgcc aacgtatccg ttcgcccgaa aggtcaagcg gaattcggca cgcgccgcga
     6181 gattaaaaac ctcaattcct tccgtttctt ggagcaggcg attaattacg aagcagaagc
     6241 gcaaatcgag attttggaag acggcggcaa agtacagcag gcaacgatgc tgtttgatcc
     6301 cgaaaaaggc gaaacccgcg taatgcgcct gaaagaagac gcgcacgact accgctactt
     6361 ccctgacccc gatttgctgc ccgtcatcat ttcagacgac cggttgcaaa aagcaaaagc
     6421 agaaatgccc gagctgccaa aagaaatggc ggcgcgtttc gtggcggatt atggcgtgag
     6481 cgactacgac gcgcgcctgc tcactgccag ccgcgtgcag gctgcctatt ttgaagaagc
     6541 cgctaaagag agcggacaag gcaagctgac tgccaactgg atgaacggcg aacttgccgc
     6601 cacgctgaac aaagaaggca tggaacttgc cgacagcccg attaccgccc cacgcctcgc
     6661 cgctctggta ggcaaaatcg ccgacggcac attaagcagc aaattggcga aaaaagcctt
     6721 tgaagccatg tgggctgaac ccgaagcctc cattgccgaa atcattgaaa aacacggttt
     6781 gcaacagatg accgataccg gcgcgattga agcgatggta gacgaagtgc tggcaaacaa
     6841 tgcaaaagcc gtggaacagt tcaaatccgg caacgaaaaa gccctgaatg cgattgtggg
     6901 gcaagtgatg aaggccagca aaggcaaagc caatcccgcg caggttcaag agctgattaa
     6961 agccaaactt gcctgataag caagatgccg tctgaaaccc aaatccgacg ttcagacggc
     7021 attttcacag tctgaccaca ttaaaacagc ataaaaaaca atattccatg aaccacacca
     7081 ttacactgcc cgaccaaacc acctttgccg ccggcgacgg tgaaaccgtt ttagccgccg
     7141 ccgcccgtca aaacctcaat ctgccccatt cctgcaaaag cggtgtctgc ggacaatgca
     7201 aagccgaact ggtcagcggc gatattcaaa tgggcggaca ctcggaacag gctttatccg
     7261 aagcagaaaa agcgcaaggc aagattttga tgtgctgcac cactgcgcaa agcgatatca
     7321 gcatcaacat ccccggctac aaagccgatg ccctacccgt ccgcactctg cccgcacgca
     7381 tcgaaaatat tattttcaaa cacgatgtcg ccctcctgaa acttgccctg cccaaagccc
     7441 cgccgtttgc cttctacgct gggcaataca ttgatttact gctgccaggc aacgtcagcc
     7501 gcagctactc catcgccaat ccgcccgacc aagaaggcat tttggaactg cacatccgca
     7561 ggcgtgaaaa cggtgtctgc tcggaaatga ttttcggcag cgaacccaaa gtcaaagaaa
     7621 aaggcattgt ccgcgttaaa ggcccgctcg gttcatttac cctgcaggaa gacagcggca
     7681 agcccgtcat cctgctggca accggcacag gctacgcccc catccgcagc atcctgctcg
     7741 accttatccg ccaaggcagc agccgcgtcg tccatttcta ctggggcgcg cgtcatcaag
     7801 atgatttgta tgccctcgaa gaagcacaag ggttggcaga ccgtctgaaa aacgcctgct
     7861 tcacccccgt attgtcccgc cccagagagt gctggcaagg aagaaaaggc cacgtacaag
     7921 acatcgcggc acaagaccac cccgacctgt cggaatacga agtatttgcc tgcggctctc
     7981 cggctatgac cgaacaggca aaaaatctgt ttgtgcaaca gcataagctg ccggaaaact
     8041 tgtttttctc cgacgcattc acgccgtccg catcataatt ccccggtata aaaaagattc
     8101 aggctttccg ttcggaacac aaaaaacttc ccgtccgtat tttccccgtg aaaaaatgcc
     8161 gtctgaaacc cgattcctgt tttcagacgg catatgtttt ttcctgttca aggcgacagc
     8221 cgctcgcgta tccagccgcc atccagcaaa cggtattgga tgcggtcgtg cagcctgctc
     8281 ggcctgccct gccaaaactc aatcaaatcg ggaatcacaa tatagccgcc ccaatgcggc
     8341 ggacgcggca cgtgcaaagg atgtttgagt ccgaccgccg ccgcctttgc caccaatacc
     8401 gccttgttcg gaatcacttc gctctgcgcg cttgcccacg cacccaaacg gctctgatac
     8461 gggcggctct caaaatattc gtccgacaat ttctccgcca gcctttcaac acgcccttcc
     8521 acgcgcacct gacgctccag ctccggccaa aaaaacgtca tcgccgcaaa tggatgagca
     8581 tccagcgaac gccccttgcg gctgtgataa ttcgtaaaga aaacaaaacc ttcagaatta
     8641 acttccttca gcagcaccat acggctgttg ggtctgccgc gtccgtcgac cgccgccaca
     8701 ttaaccgccg tcggctcgtt aacctgtgcg cgtaccgcct cgtccaacca ccgctcgaac
     8761 tgctcgatcg gattgtcggc gcaatcgcct tccgacaatt cccgtttgct gtaatcttcc
     8821 cgaatattgt gcaaatccat ttaccgctcc tcttgttatt tgaaagattc tacccccgca
     8881 cgcaaaccga tttcaaccgt tgcacaaact ttgtccccaa agccgcagcg acgatttcat
     8941 ccgcaaaacc gccgcatcag gtacaatatc gaaccgtccg actgaggacg gcattttatc
     9001 aacccgtcct gccgcatacg ccgcagaaga accgctttat caagcgagtt aggaaaaatg
     9061 atgtccaaac agcccaccag caaacgccaa tggcgcgacg gcgcagcccc gtctgccaag
     9121 aaaaccgcca aaccgttcaa aagcaaagcc cgtcccaaag acgaaacgga caaaaccgca
     9181 tcccaacctt acggacaaaa agcttcagac ggcatcaaac ctcaaaacct ccccaaacag
     9241 cgcgccgcca aagccaaaaa actcgtcgtc cgcaatccca accaaaaaat tatggaacac
     9301 gcgcgcgatt taaaagaacg ccgcagcgac ctatcgcgca tggaacccga acgcctgcaa
     9361 aaagtgcttg ccgcgtccgg cgtcggctcg cgccgcgaaa tggaagaatg gatcaacaac
     9421 ggctgggtaa cggtcaacgg caaaaccgct caactgggcg acaaagtcac ccccgacgac
     9481 cacgttaccg tcaaaggcag catcatcaaa ctcaaatggg ccgaccgcct gccgcgcatc
     9541 atcctatatt acaaacaaga aggcgaaatc gtttcccgcg acgacccgca aggccgcgtc
     9601 agcatattcg accgcctgcc gcaggccgcc agcagccgct gggtcgccat cggacgcttg
     9661 gacatcaaca ccagcggact tctgattctc accacctccg gcgaactcgt ccaacgtttc
     9721 gcccacccca gcttcgaagt cgaacgcgaa tacgccgtgc gcgtactggg cgggctgacc
     9781 ggcgaacaaa tgcgcgtcct caccgaagaa ggcgtgatgc tcgaagacgg cttggcaaaa
     9841 gtcgaacgca tccgcgaaca aggcggcgaa ggcgcgaaca aatggtacaa cgtcgtgatt
     9901 aaagaaggcc gcaaccgcga agtgcgccgc atttttgaaa gccaaggact caccgtcagc
     9961 cgcctcgtgc gcgtcggctt cggccccatc ggactgccca accgcctcaa acgcgggcag
    10021 ttctacgaac tcaaccccgc cgaagtcgcc aacatcctca aatgggcgga aatgctgctg
    10081 ccgggcgaat accgccgcaa aaaagcctaa acccgccaaa acacaaaaat gccgtctgaa
    10141 acatctgctg tttcagacgg cattttgttc gggcgttttc aggagaaaag gtcgagtgct
    10201 ttgacaaaga tcatcaccac gccgtaggcg agcggtatga cggccaatgc ccaacgccac
    10261 cagaccgata tgccgccgcc ggaaactttt tcgcccacaa ggtaagcgtc ggatatggcg
    10321 gtttcgtcat cggggttgcc gctgtgtgcg gcggttttga tgtctttttc gtggtgtttt
    10381 tcgtgtacgg atttgacggc gaggttgcac aacaaaccga taatcagcag acccgccatg
    10441 atgtacatgg ttacgctgta tgcctgtgcc gccggtatgc cgctgtcgat ttggctttgg
    10501 cgtatgtaat tgaccagtac cgggccgatg acggcggcgg ttgaccaggc cagcaggatg
    10561 cgtccgtgaa tcgcgccgac ctgataggtg ccgaacaggt ctttcaggta ggcgggaatg
    10621 gcggcaaatc cgccgccgta catggaaata atcacgcaaa agccgatgat gaacagggct
    10681 ttgctgccgc cctcgccgat ggagggaacg gcgaaataca gcagcgaacc gagtacgaag
    10741 aagatggtgt aggtgttttt gcgtccgatt ttgtcggaaa cgctcgacca caaaaagcgt
    10801 ccgcccatgt taaacaggct caggaggctg acgaagcctg ccgccgcacc tgcgccgact
    10861 gccgcctgcc tgcctatgga ggtttcggaa aagagttcct gaatcatcac ggatgcctga
    10921 cccaatacgc cgatgccggc agttacgttc aggcacaata cccagaacaa cagccaaaac
    10981 tgcggcgttt tcatggcttg ggacacgttg acatgattgc tgctgaccag cttgttttgc
    11041 gttttcggcg cggtatagcc ttcaggtttc cagccgtcgg caggtacgcg gatggtaaac
    11101 gcgccgaaca tcatcagtgc gaggtaaagc agacccaata cggcgaaggt ttcggcaacc
    11161 ccaaccgaag cagcgtttga aaaggtgttc atcagtgata cggaaagcgg cgaggccagc
    11221 attgcgccgc caccgaaacc cataatcgcc aaaccggtcg ccatacccgg cttgtcggga
    11281 aaccatttca tcagtgtgga aaccggcccg atgtagccca aacccaagcc tacgccgccg
    11341 atgacgccgt tgcccaaata gagcaggaag aggttgtgcg tacgcacgcc gaatgcggat
    11401 acgaagaagc ccaggctgaa acagcaggcg gcggcaaata tggctttgcg cggccctacc
    11461 cgttccatcc acgtaccgaa cagggcggcc gacgcgccca gcatcgcgag tgcgatactg
    11521 aaaatccaac ctacggtcgt cagcttccaa tctccggccg ccgattcggt tatgccgata
    11581 agtttggtca gcggcgcgtt gaatacggaa taggcgtaaa tctgcccgat ggcaaggtgt
    11641 accgccaatg ctgcgggcgg tacgagccaa cggttgaaac ccggcttggc aatgcttgcc
    11701 tcacggtcta aaaacttcat aacatcctct ttctgtcagt tgaaaaataa aatttcattt
    11761 gctcaatgga aacttattga aaattataaa aaaatatcgg gtcgggtttt tatccgcccc
    11821 aagatgcgcc gtctgaaaca tttcgggtgt acggaaaggt ttctgttttt tccgacaaat
    11881 tcctgcggct tttcgcttcc ggattcccgc ttttgcggga atgacgaatt aaagattatc
    11941 ttaaggtcaa gggactggat tcccgcttt
//
LOCUS       DAWKOG010000049        11910 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.049, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000049 DAWKOG010000000
VERSION     DAWKOG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11910
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.049"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            160..1344
                     /gene="coaBC"
                     /locus_tag="WJK66_000994"
     CDS             160..1344
                     /gene="coaBC"
                     /locus_tag="WJK66_000994"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEZ7578721.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTSLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILRDKKILITAGATFEAIDPVRGITNISSGKMGVALAR
                     ACRAAGAEVSLIYGQLQTELPFGISNRVQTVSAEDMHRAVHRLIDKQDAFISVAAVSD
                     YKVKNRSTQKFKKDKNAKPLSIELDENPDILASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKPTNRITIIGDDEELSFPETSKDEAAMQIVERLAVYL
                     NK"
     gene            complement(1411..3567)
                     /locus_tag="WJK66_000995"
     CDS             complement(1411..3567)
                     /locus_tag="WJK66_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ7578722.1"
                     /translation="MPVPQPTAPYDPLTAEARTLLFHTASYLKPEEQAELEKAVAYAF
                     RAHDGQTRKSGEPYITHPIAVATQLAVWHMDVQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTIAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(3660..3866)
                     /locus_tag="WJK66_000996"
     CDS             complement(3660..3866)
                     /locus_tag="WJK66_000996"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEZ7578723.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDARNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            complement(3925..4542)
                     /gene="gmk"
                     /locus_tag="WJK66_000997"
     CDS             complement(3925..4542)
                     /gene="gmk"
                     /locus_tag="WJK66_000997"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEZ7578724.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEANGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSAEGVNALAAAGYDVIL
                     EIDVQGAAQVRDALPEAVGIFILPPSFDVLAARLNGRGTDSREVIQRRLAKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLVHIVNACRLKRSRQLGFIADLLENS"
     gene            4670..5269
                     /locus_tag="WJK66_000998"
     CDS             4670..5269
                     /locus_tag="WJK66_000998"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEZ7578725.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKINIVAGLDARGFIIGAALAYHLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            complement(5315..6103)
                     /locus_tag="WJK66_000999"
     CDS             complement(5315..6103)
                     /locus_tag="WJK66_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEZ7578726.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMVETGMDAVVTINGQFALLHGKTVHEVPMDAGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALQHIASDFFADKDYFSSKPVYQMLVFAEENEMPL
                     WSDIVEQEGLKTVRWHEEAVDLLPAGMSKTDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVALGNGEQAAKEAAKYVCPSVDEDGVLRGLQDLGVI"
     gene            6428..7783
                     /gene="yegQ"
                     /locus_tag="WJK66_001000"
     CDS             6428..7783
                     /gene="yegQ"
                     /locus_tag="WJK66_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HEZ7578727.1"
                     /translation="MKAPELLLPAGGLERMRTAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVSDMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQQCPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATENDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLSGHSTAKQSQYVGHVTEIDKNGWATIEVKNR
                     FAVGDSLEIIHPNGNQTIKLEQMARKGQPVDVAPGNGIQVKIPDMQGKEKALIARVLN
                     P"
     gene            7917..8393
                     /locus_tag="WJK66_001001"
     CDS             7917..8393
                     /locus_tag="WJK66_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HEZ7578728.1"
                     /translation="MLAVCQSGSAPKNAHTASPGTPAGESCKVIRGDTAGSGKNIIYR
                     CCNGQAAINAAIADGVLESGIPVSFGGGGVPVANGRNLVTRQAANGIGKSDSEAYERA
                     LINAARKFQQTAGKLGGRGVTGFHSYFDKQPLQGGQYDCQAGSFHVRVVMRANVVR"
     gene            complement(8646..8987)
                     /locus_tag="WJK66_001002"
     CDS             complement(8646..8987)
                     /locus_tag="WJK66_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEZ7578729.1"
                     /translation="MPQVKLIITRPAQSNLVRIYNFLNDLGAEHQANTVMRLLKNSFV
                     STQGSPSNGKPYDLSVAGETLQNVREVIVPYGKSGYSYLFWHDKSGKQILILTVRHFR
                     ENAYRLSFPFL"
     gene            complement(8975..9262)
                     /locus_tag="WJK66_001003"
     CDS             complement(8975..9262)
                     /locus_tag="WJK66_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578730.1"
                     /translation="MMLTNQEVTGVKLGYRKAVLQELARDFGGSVHSIVLDAVDGHIE
                     RLKAKQAYDKRAMQAFQDYRETGLHITLNEFADWVDGLGGDESKDMPECRK"
     gene            9332..9442
                     /locus_tag="WJK66_001004"
                     /pseudo
     CDS             9332..9442
                     /locus_tag="WJK66_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
     gene            complement(9632..11091)
                     /locus_tag="WJK66_001005"
                     /pseudo
     CDS             complement(9632..11091)
                     /locus_tag="WJK66_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250318.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
     gene            complement(11222..11851)
                     /locus_tag="WJK66_001006"
     CDS             complement(11222..11851)
                     /locus_tag="WJK66_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEZ7578731.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLKDLGEEPYKFDKELPYE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALGLSPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
BASE COUNT         2917 a         3245 c         2756 g         2992 t
ORIGIN      
        1 aatcccttcc tccccctcct ctgactggtg cgcctttgtg aatatgccgt ctgaaactcg
       61 gggactcaga cggcattttg tatccaaacg gtatctaatg tatccacact ttgttataga
      121 atggctgctg ttttttcttc gtaattagaa attgtcaaaa tgggcaaaca tattctttta
      181 ggtgtaacgg gcagtattgc ggcgtataag tcttgcgagt tggttcgtct gctgaaaaaa
      241 caggggcatt cggttacggt ggttatgagc cgctcggcaa ctgaatttgt ttctccgctg
      301 acttttcagg ctttaagcgg caatcctgtc ctgaccgaca cgcacggcgg caacggttca
      361 aacggtatgg aacatatcaa cctgacccgg aatgcggatg tttttctgat tgcgccggca
      421 agtatgaata ccgtggcaaa aatctgtaac ggcgtggcag ataacctact gaccagtctg
      481 gcagccgcac ggaaatgtcc tcttgccatc gctcccgcga tgaatgtgga aatgtggctc
      541 aaccctgcca accaacggaa tatcgcacaa ctggtttcag acggcattac tgtctatatg
      601 ccgggcttgg gcgaacaggc ttgcggagaa aatggtatgg gaaggatgcc ggaacctgcc
      661 gaattgctgg atttgcttcc agacttgtgg acaccgaaaa ttttaaggga caagaaaatc
      721 ttgattaccg caggtgcgac atttgaagcc attgaccctg tcaggggcat cacaaatatt
      781 tccagcggga aaatgggcgt agctttggcg cgggcgtgtc gtgccgccgg tgcggaagtc
      841 agcctgattt acgggcaact tcaaaccgaa ctgcctttcg gtatatccaa tagggttcaa
      901 accgtcagtg ctgaagatat gcatcgtgca gtgcatcgtt taatcgacaa acaagatgct
      961 tttatttctg ttgccgccgt ctcagactat aaggttaaaa acaggagtac gcaaaaattc
     1021 aaaaaagata aaaatgccaa accattatcc atcgaattgg atgagaaccc cgatattttg
     1081 gcttctattg cctcattgcc gaacccgccg ttctgcatcg gttttgccgc tgaaacggag
     1141 aatgtaatgg catatgcgcg agaaaaacgt attaagaaaa aaataccagt gatcgttgcc
     1201 aatgatgttt caatcgcgat gggcaaaccg accaaccgga ttaccattat cggggacgac
     1261 gaggaactgt cttttcccga aacaagtaaa gatgaagcgg caatgcagat tgttgaaagg
     1321 cttgccgtat atttgaacaa ataagcaatt gaacggataa accataaagc gggttgcctg
     1381 ttaatcaaaa ggcaacccgt tttacctgct tcaacttctg atgactttgc ggatatatgg
     1441 aatactatgc agattttgaa taatctgatt caattgattc agattcttga ctttcaataa
     1501 gaatttgaat tcgacaaaac cttccgttcc cgactgggat ttagacggtg tttcgaccga
     1561 ctcaatgtcc gcaccggaat cggaaatcgc ttgcgccatt aatgccagca agccgtggct
     1621 gtcttccgat tggacttgaa gcccgacacg gtagttctgc ccgttcatat tttcccagtc
     1681 tgcatccagc tgctgttcgg gatcggactt taacaacgtc gggcaggtat ccctatggat
     1741 aatcatgcct tttcccttaa ccaacagcaa acggatggaa tcgccgggaa ccggatggca
     1801 gcactctgca aaatgaatat gcccgctttc ctgaccatca actttaatgg aactgagcct
     1861 gacctcgctg ccgaaatgct cccctgccaa ctcggcaatg tgcatggcga cataaacagg
     1921 cagggtatgc cccataccga cgttgtacag cacttcctca aatgaagtct gtttgtcgtt
     1981 gagatcggca aggtattttt ctttaatgcc gtctgaaagc aggacatctt tgggcagcaa
     2041 actggacagg gctttttgta agaggctctc tcccaaaacg accgcatcgt gccggttaag
     2101 gtttttaata tattggcgta tggcactgcg cgccctgcct gacacggcga aattcaacca
     2161 tgcaggattg ggtttggcgt gttcggatgt gataatttca acagaatcac cggttttgag
     2221 cttcgtacgc aacggcatca tgatattgtt gatacgtgcg gcaacggttt tgtgcccgat
     2281 atcggtatgc accgcataag caaaatcgac aggcgttgcc cctttgggca aagttaggat
     2341 ttttcctttt ggcgtaagga tgtagatttc gttcggaaac aaatcgactt tgacgtgttc
     2401 gagaaactca atggcattgg cactgcttgc ctgcaaatct aagatatttt tcagccaccg
     2461 gtttgtgtga agcaccgcct gatcgaccgt cttagaatat gatttatagc tccaatgtcc
     2521 ggcgattcca ccttcggcaa cagcatccat ttccttggta cgtatctgaa cttcaatcgg
     2581 caagccgtaa gggccgacca aagtcgtatg cagactttga tacccgttgc ttttcggaat
     2641 ggcgatatag tctttgaacc gcccgggctt gggctgatag agggtgtgca atgcaccgag
     2701 tgcggcataa caggctggaa tgctgttgac aatgacgcgg aaaccgtaaa tatccataac
     2761 ctcggcaaag cgcagctttt tcgccatcat tttctgatgg atgccgtaca ggtttttttc
     2821 cctgcctttg attttggcct ctatattcgc gcctaccagc cgctggccga atgcgcgcaa
     2881 gactttgccg acaacgtcct gccggttctt ccggctcttg tccatcgctt tttttaaagt
     2941 ctcgtagcgg ttgggatgca ggttttggaa cgataaatcc tgaagctctt gatatgcgtt
     3001 attcaaacct atacggttgg caatctgtgc atagatttca agggtttccc ttgcaatccg
     3061 gcggcgtttg tccgggcgca tcgaaccgag cgtccgcata ttgtgcaggc ggtcggcaag
     3121 tttgacgacg atgacgcgca catctttggt catcgccaaa atcagtttgc ggaaactctc
     3181 cgcctgatgt tccgcatgat cttcaaattt gagtttttcg agtttggaca gcccgtccac
     3241 catctcggca atcgtattgc cgaacaccgc cgccatttcc ccttttgtca cgcccgtatc
     3301 ttccaatacg tcgtgcatta cacctgcaca aagaccctgt acgtccatat gccaaaccgc
     3361 aagctgtgtc gcaacggcaa tcggatgcgt aatgtatggt tcgccacttt tgcgggtttg
     3421 cccgtcgtgg gcgcgaaacg cataggcgac ggctttttca agctccgcct gttcctcggg
     3481 cttgaggtag gaggctgtat ggaaaagcag ggtacgcgct tcggcggtca gagggtcgta
     3541 aggtgcggtg ggttgaggga caggcatttc agacggcttt cggaacatat tttatttttc
     3601 gtttcaaacc gtcggactgc acggcggcag aatgtgccga cgtgcgtttc cggcagaatt
     3661 tatttattgc gcgtcaacag ttccgtaccg atatgcccgg cggcgatttc ccttaaggcg
     3721 gtaacggtcg gtttgttatt gcgggcatcg tccacaagcg gcgtgttgcc gttctcaagc
     3781 tggcgggcgc ggcgggccgc taccaatgtc aggtcaaaat ggttggaaat ttttccggta
     3841 cagtcttcgg tggtaatacg tgccatatta tttgctttct ttcaaaaata tttaaattgg
     3901 gaaaccgggt attttcgccg ttttctagga attttccaac aaatctgcaa taaatcccag
     3961 ttgccgcgac cttttcagac ggcaggcatt cacaatatga accaaatcct cctccgcccg
     4021 cgccaagtcg tcattgacca cgacaaagtc gaacaatacg gactgttcga tttcatgcct
     4081 tgccttcgca agcctccttt ggataacttc ccgactgtcc gtcccgcgtc cgttgagacg
     4141 cgcggcaagc acatcgaaag aaggcggcag gataaagata ccgacggctt cgggcagcgc
     4201 atctcgaacc tgcgccgcgc cctgaacgtc gatttccaaa atcacgtcat aacctgccgc
     4261 cgccaacgca ttcacgccct ccgcgcttgt gccgtaatag ttgccgaata cgtctgcgta
     4321 ttccaaaaaa gcttcctgcg cgataagcga ctcaaactct tctttggaaa caaagtgata
     4381 atgtacgccg tttgcttcgc cttcacgcgg cgggcgcgtc gtatgcgata cggaaacgcg
     4441 caaaccgtta tggtttgcca acagccgcga caccagcgtg gttttgcccg tgccggaagc
     4501 ggccgaaatg ataaagatgt tgccttttcg ataagcggac atatttttta cctgtatatt
     4561 ttccagccga ttgtatcaca acggacaccc agtttcctat ttgccgatgc ccatattttg
     4621 ccgctattgt tttgattgat ttggcaagcg gcaggctgac ggctacaata tggcgttaaa
     4681 aacatcaaac ttggaacacg caatgctggt tcatcccgaa gctatgagtg tcggcgcgct
     4741 tgccgacaaa atccgcaaaa tcgaaaactg gccgcaaaaa ggcatcttat tccacgacat
     4801 cacgcccgtc ctgcaaagtg cggaatactt ccgacttttg gttgatttat tggtttaccg
     4861 ctatatggat cagaaaatca acatcgttgc cggtttggac gcgcgcggct tcattatcgg
     4921 cgcggcactc gcctaccatc tcaacgtcgg ttttgtcccc atccgcaaaa aaggcaagct
     4981 gccttttgaa accgtatcgc aaagctacgc gctcgaatac ggggaagctg cggtggaaat
     5041 ccacaccgat gccgtcaaac ccggttcgcg cgtgctgctg gtcgatgatt tggttgccac
     5101 gggcggcaca atgcttgccg ggctggaact gatccgcaaa ctcggcggag aaattgtcga
     5161 agctgccgct attttggaat ttaccgacct tcaaggcggc aagaatatcc gtgcaagcgg
     5221 cgcgccctta tttaccctgc ttcaaaacga aggctgtatg aagggctgaa aaccgaccct
     5281 gccgtctgaa accggcaggg ttgttattat gcgttcaaat cacgcccaaa tcttgcaagc
     5341 ccctcaacac gccgtcttca tcaacgctgg ggcaaacata tttcgccgct tctttcgccg
     5401 cctgttcccc gttgcccagt gccacgccga acccgacttc agacagcatt tccacatcgt
     5461 tcaaaccgtc tccgaacgcc atcacgtccg ccatttccaa acccaatgct tcaaccacgc
     5521 ttctgatgcc gtctgttttc gacattcccg caggcagcag atcgaccgct tcctcgtgcc
     5581 agcgcaccgt tttcaagcct tcctgttcca caatatccga ccagagcggc atttcgtttt
     5641 cctccgcaaa caccagcatc tgatacaccg gtttgcttga aaaataatcc ttatcggcaa
     5701 aaaaatcgct ggcgatatgc tgcaaggcgc ggcacacgca ttccgacagc gcggacacgg
     5761 cgatcccctc tccgccgaca aacgcataat ccatgcccaa gccatccaaa tgcgcgcaaa
     5821 ccctgcccat caaaccggca tccatcggta cttcgtgcac agtttttccg tgcagcagcg
     5881 caaactgtcc gtttatcgtt accacggcat ccatacccgt ttccaccatc atgtccctga
     5941 ccttttcggg aatcgtcgcc aaagaccgcc ccgttgccaa cgccgtcaat atacctttgc
     6001 cgcgcaaagc cgccaccgcc gttttcacgg aaggacgcaa ggtatccgta tatttgcggt
     6061 acagcgtatc gtcaatatcg aaaaacacga ttttgggatt catcacattc tctctcgcat
     6121 tcaaattgcc gcattgtatc ccaagcaggc aaatacttga taaatcctta taaatttccc
     6181 ggcaaaattg accgcgaaca cgaaaaggcg gataatccgc ccatcctcaa acccttttca
     6241 gacggcattt gcagcaatgc cgtctgaaac atttttacaa agcacacaaa tcatgtttca
     6301 acacacagga cgacacataa agcgtcgccc tatatgttgc cctgattcgg aaagggttac
     6361 acccctccca aataaagtct gattctaccg ccctaaaggg cggggtttca accgaaaagg
     6421 aaacacgatg aaagcacccg aactcttatt gcccgccggc ggattggaaa gaatgcgcac
     6481 cgcctacgac tacggcgcag atgccgttta tgccggcagc ccgcgttact cactgcgcgc
     6541 ccgcaacaac gaatttgcca aactcgacgt tctcgagcaa ggcattaaag aagcgcacga
     6601 gcgcaacaaa aaattctttt tgaccgtcaa caccctgccg cacaactcca aactcaaaac
     6661 cttcgtttcc gatatggagc cgctgattgc catgaaaccc gacgcgctga ttatggcgga
     6721 tccgggtttg attatgaccg tgcgcgaaaa atggccggaa atgccgatcc acttgtccgt
     6781 acaggccaac accaccaact actggggcgt gaaattctgg cagaatatcg gcgtcgaacg
     6841 catcatcctg tcgcgcgaat tgagtatgga agaaatcgcc gaaatccgcc aacaatgccc
     6901 cgacatcgaa ctcgaagtct tcattcacgg cgcattgtgc atcgcctact ccggccgttg
     6961 cctactctcc ggctacttca accaccgcga ccccaaccaa ggcacctgca ccaactcctg
     7021 ccgttgggac tacaaagtcc acaacgctac ggaaaacgat gcgggcgatg cccagcttct
     7081 gcaaggtttt aactttgaaa aagcccaaga agaagccaac caaaactttg aaggcatcaa
     7141 cggtcaaaaa cgccatccct acgccgacaa agttttcctg attgaagagt ccaaccgccc
     7201 gggcgagatg atgccgatta tggaagacga acacggcact tacatcatga actccaaaga
     7261 cctgcgcggc attgaagtgg ttgagaaact cgccaaaatc ggcgtggaca gcctcaaagt
     7321 cgaaggccgc accaagtcgc tctattatgt cgcacgcgtc gcccaatcct accgcaaagc
     7381 gattgacgat gccgtcgcag gccgtccgtt tgattacagc ctgttgagcg aactcgaagg
     7441 ccttgccaac cgcggctaca ccagcggctt cctcgaacgc caccaaaccc aagactatca
     7501 aaactacctc agcggccatt ccaccgccaa acaaagccaa tacgtcggac acgttaccga
     7561 aatcgacaaa aacggctggg cgaccatcga agttaaaaac cgcttcgccg tcggcgattc
     7621 actcgaaatc atccacccga acggcaacca aaccatcaaa ttggaacaaa tggcccgaaa
     7681 aggccagcct gtcgatgtcg cacccggcaa cggcattcag gtcaaaatcc ccgatatgca
     7741 gggcaaagaa aaagccctca tcgcacgcgt gttgaacccc tgagccacaa tgccgtctga
     7801 accccgtttt cagacggcat tttgttgaat cttacggtac aatggcaatg atttcccacc
     7861 gcaagcaagg atatccgaca tgaaaaaaaa tcctcatact ctccgccgcc gccctgatgc
     7921 tggcagtctg ccaatccggc agcgcaccta aaaacgcgca cacggcatcc cccggcacac
     7981 cggccggaga atcatgcaaa gtcatccggg gcgacaccgc aggaagcggc aaaaacatca
     8041 tttaccgttg ctgcaacggt caggcggcaa taaatgcagc cattgcagac ggcgtattgg
     8101 aatcagggat tcccgtcagc ttcggcggcg ggggcgtacc ggttgcaaac ggcaggaatc
     8161 tagttacccg tcaggcggca aacggcatag gcaaatccga ctcagaagcc tacgaacgcg
     8221 ccctaatcaa tgccgcccgc aaattccaac agaccgcagg caaactcggc ggacgcggcg
     8281 ttaccggctt ccacagctac tttgacaagc aaccgctgca aggcgggcaa tacgactgtc
     8341 aggcaggctc gttccacgtc cgcgtcgtca tgcgcgccaa tgtcgtccgt taacatatcg
     8401 gcaatcaaaa aatgccgtct gaaacatttt tcagacggca tttttaatcc ccttgcctta
     8461 ttgtgcggca gattcagatc gggacactcc atacttccat atctgcaccg ttcaaacatc
     8521 ccgcctgcgg aacggaatca aagcggcagt ccggttatgt tcttgaaggc ggtttatccg
     8581 aacgtgccgc aacaaagccg aaacgcattg ccccgcacaa gtgaaagccg ccaaaccaaa
     8641 taccgttata aaaaaggaaa gctcaggcgg taggcatttt cacggaaatg cctcaccgtc
     8701 aaaatcaata tttgcttacc tgatttatcg tgccaaaaca aatagctata accgcttttg
     8761 ccataaggca cgattacttc ccttacattt tgcaaagttt cccccgccac cgacaaatca
     8821 taaggtttcc cattactcgg gctgccttgt gtagaaacaa aagaattttt aagcagccgc
     8881 atcactgtat ttgcctgatg ttccgcaccc aaatcattca aaaaattata aatcctgacc
     8941 aaattagatt gagcggggcg tgtaattatc agctttactt gcggcattcg ggcatatcct
     9001 tcgattcatc gccacccaag ccgtccaccc agtctgcaaa ctcattaagc gtaatatgca
     9061 atcctgtttc ccgataatcc tgaaaagcct gcatcgcccg cttatcgtag gcttgtttgg
     9121 ctttcaagcg ttctatatga ccatccaccg catccaatac gatactgtgc acactgcccc
     9181 cgaaatcccg ggccagctct tgtaaaacgg ctttgcggta tcccagtttg acaccggtta
     9241 cttcctgatt ggtaagcatc attattttcc cataaatcaa acgtctgaca cggcattata
     9301 aacacaatgc ggcatctgcc gccacccttg cggacgcggc gttaccggct tcctcagcta
     9361 tttcgacaag cagccgctgc aaggcggaca atacgactgt caggcaggct cgttccacgt
     9421 ccgcgtggtc atcgtccgtt aaacatatcg gcaatcaaaa aatgccgtct gaaacatttt
     9481 tcagacggca tagttcataa atcaaatcgg taatacagat aatcgttcaa ttaggattgc
     9541 ttctgcaggt gggacaacac atcaaggtgt tgcccgatgc cctgtcggaa ggaaacgcca
     9601 ctgttcccga ctgtgaaaca caggataaat atcaacggac gtttgcttct tttttgcctg
     9661 caaatacgcc gaagccgttg tcgctaaaac catcattttt atcaggggtt ttattgaaac
     9721 tgtatctgcc cgccatttca tctccattag agccataaaa cttcccttca acatctcctt
     9781 gttcttttgt atcccatttg cctttaaagc cacttccctc aatcttggca gtaaattccc
     9841 ttgttcccat atacaaacca tcgccactat tgattgtccc ttttacagac ttctgaccga
     9901 aatcgacttc tgctgcaaac gtacctgtcg tcctatatga atttccttcc tccaaccagt
     9961 ctgtttcatc agtaacattg cgggaacgga aatgcagcac cttaccttga tatttagctt
    10021 tgccttccgg cagattattt tctgtcggct gaccttgtac aaaaagagta tatgagccat
    10081 cttttaattt aacagcccca taaatcagat tcttatattg gttttgagaa accattacct
    10141 tatcaaaatc actcatcaaa ccggggtgat ttctacttac tggctcatta gctttcgtta
    10201 aatcaatagt ttctccgcca atcaccaacg ccgtgatttc tcctgtgccg tcaaaatatt
    10261 tctcgccaaa cacgcctgtt ttaggttgag tatcggcagc actttggact tgtttgtctt
    10321 gggaatccgg attttcactt acaaactttt cctccgtatc atcttgagat ttcgcaactt
    10381 tctcgacatc ttgattgccg ccatccgacc ctacggcatc actgccgggc atatcacggt
    10441 tgttttcagg ctgcttgccg tcggcagcac tttgggcttg tttgtctggg gaagacggag
    10501 ttacgcttac agcctttccc tccgtatcat cttgagattt cgcaactttc tcgacatctt
    10561 gattgccgcc atccgaccct acgggatcac tgtcgggcat acgttctttt tcaggctgct
    10621 tgccgtcggc agcactttgg gcttgtttgt ctggggaaga cggagtttcg cttacagcct
    10681 ttccctccgt accatcttga gatttcgcat ctttctcgac atcttgattg ccgccatccg
    10741 accctacggc atcactgtcg ggcatacgtt ctttttcagg ctgcgtgtcg gatgtgccgt
    10801 ttgccggcac attatcatta tgttggggcg catcattttg cactccgcca tcagcgcctg
    10861 cctgcgcggt ttccccttct ccctgcgtat ctttagacgg tatttctgtt ttggattccc
    10921 ctccgtcccc gcccgaggta ggatttaccg tttcagcggg gggggggtaa ctttatttga
    10981 cggcgtttct gaggatttga ctacaggtgt accccctccc cctgaaatac aggcggaaag
    11041 ggcgaggatg gatgccattg caagcaggct cggtttgaaa ggaatcgtca taattatctc
    11101 ctatggcata tttttaatga aaaatatgat aattatttat gtttaatggt ttaaaaatac
    11161 cgccatacgc ggcaggaaat acggctctgc ttcacaaatc ggggcagggt cgtaaaacac
    11221 attagtgcct gtgcggcatc attccttccg gtgcttcggc atcggctgcc aagccgaagg
    11281 tttcgcgcaa cacgactttg tagaatgcaa aggcttcgcg cgcgccttgg atggcttcgg
    11341 cttcggcttc ggggcttaag cccaatgcgt tcagatgctc gacgaaggcg cgccagtgtt
    11401 tgccgcgccc gtcgggatgc ggagcgaggt ggcgcgcgcc gtgttcgccg ttgtaatcga
    11461 gtttttgggc gtgtttgaac aaaaacgccg cgcccaaatt ggatccttcg gcgcaataaa
    11521 gccagccgat tgctttgttg ccagtttcgt atggcaattc tttgtcgaat ttgtaaggct
    11581 cttcgcccaa atctttcaag tcttgcgtta cggcatcgta tcgcgccatg tattccagct
    11641 cgggaatggc tttgtttaat tcggcatctt tatagatgtg gtcgacggcc ttgtggaaaa
    11701 cggattggag tttcaaaaat ttgatgtagt tttctttgct gacaaacggt tggacagaca
    11761 taacgaggtt atccacgctg tcgtgaaccg ccgtggtatc cgccttcaga cgtttggcaa
    11821 acgtcagggc ttgattttcg gtttcactca taagttttcc tttgtcggta agggataagg
    11881 atgcggggcg ggcaaaatcc gcacttgccc
//
LOCUS       DAWKOG010000050        11610 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.050, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000050 DAWKOG010000000
VERSION     DAWKOG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11610
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.050"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(133..468)
                     /locus_tag="WJK66_001007"
     CDS             complement(133..468)
                     /locus_tag="WJK66_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ7578732.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            597..1502
                     /gene="mtrA"
                     /locus_tag="WJK66_001008"
     CDS             597..1502
                     /gene="mtrA"
                     /locus_tag="WJK66_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HEZ7578733.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGSGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     MVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(1564..2052)
                     /locus_tag="WJK66_001009"
     CDS             complement(1564..2052)
                     /locus_tag="WJK66_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ7578734.1"
                     /translation="MKKYLIPLSIVAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            2199..3101
                     /locus_tag="WJK66_001010"
     CDS             2199..3101
                     /locus_tag="WJK66_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ7578735.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPCWRVVFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(3142..4272)
                     /locus_tag="WJK66_001011"
     CDS             complement(3142..4272)
                     /locus_tag="WJK66_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7578736.1"
                     /translation="MAKHLPLAVLTALLLAACGGSDKPPAEKPAPAENRNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPSHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            4477..7101
                     /gene="alaS"
                     /locus_tag="WJK66_001012"
     CDS             4477..7101
                     /gene="alaS"
                     /locus_tag="WJK66_001012"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEZ7578737.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAAFSME
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVKEMGDAYPELKEKQVQIEEALKNEESRFAQTLETGMALLENALAKGSKKLDGEI
                     IFKLYDTYGFPYDLTADICRERNIELDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGEQVDELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFSGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGRHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAVLANVAV
                     NAAIMSMEDAQKTGAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTNLTGKSDNA
                     VILLAAVNDGKVSLCAGVSKPLTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAG
                     KLPEMLVSVESWLCQKLS"
     gene            7163..7894
                     /locus_tag="WJK66_001013"
     CDS             7163..7894
                     /locus_tag="WJK66_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578738.1"
                     /translation="MNTKTELQKLLEEDISTLTETLICADALPPRYVRSIATPIVRRW
                     LIDKQLNILAKEIGLTIELPIFDTSLVFEKLSTLENKVNFYMAGGVYLGGEFISSIYH
                     SSQEFSGEPIIYAEPNIILCPAEKFLTLKRVFHNGNIFNMNQIITFLSNKQGGVHFDK
                     NYDKYKTWQVAIEKAANFLKLGNPYNEDKLSLSEEHDTILVVLPLEKGYEWNCLEIEV
                     LSAAQSLANIYCNKVRLIDGHVWKE"
     gene            8603..11014
                     /locus_tag="WJK66_001014"
     CDS             8603..11014
                     /locus_tag="WJK66_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ7578739.1"
                     /translation="MEELPEYITRGVFDFMFGIEAKVRQIQEEANIQAAATIGRLHDA
                     LKEEGIYEEHELRLFITRLLFLFFADDSAVFRRNYLFQDFLENCKEADTLGDKLNQLF
                     EFLNTPDQKRSKTQSEKFKGFEYVNGGLFKERLRTFDFTAKQHRALIDCGNFDWRNIS
                     PEIFGTLFQSVMDAQERREAGAHYTEAANIDKVINGLFLENLRAEFEAVKALKRDKAK
                     KLAAFYQKIQNLQFLDPACGCGNFLIVAYDRIRALEDDIIAEALKDKADGLFDSPSVQ
                     CRLKQFHGIEIDEFAVLIARTAMWLKNHQCNIRTQIRFDGEVACHTLPLEDAAEIIHA
                     NSLRTPWQAADYIFGNPPFIGSTYQTKEQKNDLESICGHIKGYGLLDYVCNWYVKAAG
                     IMAQHPQVQTAFVSTNSICQGQQVEILWGSLLNQGIEIHFAHRTFQWTSQAAGKAAVH
                     CIIVGFRQKPPMPSEKTLYDYPDIKGEPEKHAVANINPYLIDAPDLIIAKRSRPIHCE
                     PDMVNGSKPTEGGNLILSTAEKDALIAAEPLAEQYIRPFIGADEFLNGKTRWCLWFHG
                     VSDVKRNHDLKQMPQVQARIQAVKTMREASSDKQTQKDAATPWLFQKIRQPSDGNYLI
                     IPSVSSESRRFIPIGYLSFETVVSNLAFILPNATLYHFGILSSTMHNAFMRTVAGRLK
                     SDYRYSNTVVYNNFPFPESCRLPSENDRPDPLRAAVEAAAQTVLDARGQYRREAQEAG
                     LPEPTLAELYAPDAGYTALDKAHATLDKAVDKAYGYKTGKNTDDEAERVAFLFELYRK
                     AAAIA"
BASE COUNT         2967 a         3214 c         2736 g         2693 t
ORIGIN      
        1 tcacgcttac cccttgtttc tcaaatgccg tctgaaataa gcggcttaat atattgttta
       61 cagtattggg aagcataaca gacaaaatgc cgtctgaaat attttcagac ggcatttctt
      121 atccgaaacg gattattttt gcgtttcaac cgcttccaat gcacgcaggg cgtaagtgta
      181 agcggcaccg gcattcaggg cgatggcggt tgccaatgcg cctgcgattt cgctgtcggt
      241 cgcaccggct ttggcggcag cggcggcgtg aacgctgatg cagctctcgc aacgagtggt
      301 aatggcaacg gcgatggcaa tcagttcgcg cgttttggca tcaagtgctt cggcggcggc
      361 ggcctgttcc aatgcgccgt aggcctgcag cattttagga tgcgccttac ccagctcgcc
      421 gaacgatttt ttaaccaatg cggtatgttc tttccaatct ttaaacattt tcttttcctt
      481 tctcttgcgt ttaaccctga tacgcgcttg cgtatctgtt ttcgatgtgc gtattattgc
      541 aattattcag ttgtgtttct cgtttaatca tctcatttta tggttcaaaa agatttatgg
      601 acattctgga caaactggtc gatttcgccc aattgacggg cagtgtggat gtgcagtgcc
      661 ttttgggcgg acaatggtcg gtacggcatg aaaccttgca acgcgaagga ttggtacaca
      721 ttgttacatc gggcagcggc tatctctgca tcgacggcga aacttccccg cgtccggtca
      781 gtacagggga tattgtattt ttcccgcgcg gcttgggtca tgtgttgagc cacgacggaa
      841 aatgcggaga aagtttacaa ccggatatgc ggcagcacgg tgcgtttacg gtcaagcagt
      901 gcggcaacgg acaggatatg agcctgtttt gcgcccgttt ccgctacgac acccacgccg
      961 atttgatgaa cgggctgcct gaaaccgttt ttctgaacat tgcccatccg agtttacagt
     1021 atgtggtttc aatgctgcaa ctggaaagca aaaaaccttt gacggggacg gtttccatgg
     1081 tcaacgcatt gtcgtccgtc ctgctggtgc ttatcctgcg cgcctatctc gaacaggata
     1141 aggatgtcga actctcgggc gtattgaaag gttggcagga caaacgtttg ggacatttaa
     1201 tccaaaaggt gatagacaaa ccggaagacg aatggaatgt cgacaaaatg gtggcggctg
     1261 ccaatatgtc gcgcgcgcaa ctgatgcgcc gtttcaaaag ccgggtcgga ctcagcccgc
     1321 acgcctttgt gaaccatatc cgcctgcaaa aaggcgcgtt gctgctgaaa aaaaacccgg
     1381 attcggtttt gtcggtcgca ctgtcggtag gctttcagtc ggaaacgcac ttcggcaagg
     1441 cgttcaaacg gcaatatcac gtttcgccgg gtcaataccg gaaagaaggc gggcaaaaat
     1501 aaatcggggc ttcaaacgca aatgccgtct gaaaaggctt tcatacagca tttgcgtacc
     1561 gcgtcatttc aagggctgca tcttcatcac ttccatcaaa aagttggtaa atgcggggtt
     1621 gttgggtttg acatccatat ttttccaacg ctgctgccag ccgcgcaagg cattctggat
     1681 atacagcttg gactgttccg tattgattgc gccccgctgg ctgtctatcg ccgaacgcag
     1741 gtagatttca tacatactgt catcgacggc attgcgtccg accaggcgtt ttctgaagtt
     1801 gttcagatat tgcgccgcct gaaccttggt cattttaccg atacccacct gatagcccaa
     1861 gcgcgtcgct tcatcgctga ttttggcaac atccgtccaa tgcgaagagg caaggcggaa
     1921 accttttgca ggtgcttccg ttttgacggt attgatagga ttcacgggga tttccgtcaa
     1981 tgtgggcaca taaatagact ggcagccgga aagaactgcc acaatggaaa gagggataag
     2041 gtattttttc atgcccccat tataatcaag tttgccttga gaaaacaaat tgttcggcaa
     2101 gaaaaataaa atttcggcat cagaagcagg caaaaacaca ttccacaagc cttgccgcaa
     2161 ggtttacaat ccgaccgtcc ttatcgcaac gaccgtttat ggataccgca aaaaaagaca
     2221 ttttaggatc gggctggatg ctggtggcgg cggcctgctt taccattatg aacgtattga
     2281 ttaaagaggc atcggcaaaa tttgccctcg gcagcggcga attggtcttt tggcgcatgc
     2341 tgttttcaac cgttgcgctc ggggctgccg ccgtattgcg tcgggacacc ttccgcacgc
     2401 cccattggaa aaaccactta aaccgcagta tggtcgggac gggggcgatg ctgctgctgt
     2461 tttacgcggt aacgcatctg cctttggcca ccggcgttac cctgagttac acctcgtcga
     2521 tttttttggc ggtattttcc ttcctgattt tgaaagaacg gatttccgtt tacacgcagg
     2581 cggtgctgct ccttggtttt gccggcgtgg tattgctgct taatccctcg ttccgcagcg
     2641 gtcaggaaac ggcggcactc gccgggctgg cgggcggcgc gatgtccggc tgggcgtatt
     2701 tgaaagtgcg cgaactgtct ttggcgggcg aaccctgctg gcgcgtcgtg ttttaccttt
     2761 ccgtgacagg tgtggcgatg tcgtcggttt gggcgacgct gaccggctgg cacaccctgt
     2821 cctttccatc ggcagtttat ctgtcatgca tcggcgtgtc cgcgctgatt gcccaactgt
     2881 cgatgacgcg cgcctacaaa gtcggcgaca aattcacggt tgcctcgctt tcctatatga
     2941 ccgtcgtttt ttccgccctg tctgccgcat tttttctggg cgaagagctt ttctggcagg
     3001 aaatactcgg tatgtgcatc atcatcctca gcggcatttt gagcagcatc cgccccactg
     3061 ccttcaaaca gcggctgcaa tccctgttcc gccaaagata aaaaatgccg tccgaacatc
     3121 cttcagacgg catatcgggc tttatttccc cgccttcaca tcctgccact ggcgcaccat
     3181 aaacttcaat gccgccggct ggataggcac catgataaag ctgtttttca aatcctcctc
     3241 ggttgggaaa atcgtattgt cgtttttaaa ttcgtcttcc atcagctcac gcgccggctt
     3301 gctcgaaggc gcataagtaa cgaaattgcc gtttttcgcc gacacttccg ggtcgaggaa
     3361 gtcgttgatg tatttgtgcg cgttggcgac gtttttcgca tctttcggaa tcacgaaaga
     3421 atccacccaa atccccacgc cctctttggg catcatcacg cggatttttt ccttgccgcc
     3481 cgcttcttcg gcacggcgtt tggcgatgtt caaatcgccg ccgaaaccga ttgttacgca
     3541 ggtatcgccg cgcgccaaat catcgataaa gccggacgaa gtaaagcgtt tgatattggg
     3601 gcggtttttc ttgagcaggg cggttgcctc cctgatgtct tccgtattgc tgctgttcgg
     3661 gtttttaccc aaatagttca acaccatagg atagatttcc gccgcgctgt ctaaataact
     3721 gataccgcat tgtttgagtt tggacgtgta ttcggggtcg aacaccaaat cccactggtt
     3781 gtccggcagc ttgtccgtac ccaaagcctt tttcacgcgt tcggtattaa tggcgaaggt
     3841 atttgtcccc caataaaacg gcacggcgta ttcgtggctg ggatcgaccc cgtccatcag
     3901 cctcatcatt tcgggattga ggtgtttata attgggaatc agcgatttat cgattttctg
     3961 atacgcacct gccttaatct gcctgcccac aaacgcattg gacggcgcga caatgtcgta
     4021 accggatttt ccggtcagca ccttgctttc cagcgtttca tcgctgtcgt acacatcata
     4081 agtaaccttg atgccgtttt tcttttcaaa atcggcaacg gtttccggat cgacgtattc
     4141 cgaccagttg taaattttca atacgtttcg gttttccgcc ggcgccggtt tttcggcagg
     4201 cggtttgtcc gaaccgccgc acgctgcaag cagcaaagca gtcaggacgg cgagtggcag
     4261 atgtttggcc attatcattc cttgcatatc gggttggaga aagcggtcat tatagccgat
     4321 attggcaaca gggcttcaga cggcattcaa aatcccgcca cacccttccg aaaaccgccg
     4381 cttccatagc tagaaacagg gatttgcggt aagataccgc cgttcgtttt ccctgctttt
     4441 accatgacaa gacatttgag agacattgaa aaaattatga aaacctccga actgcgccaa
     4501 aaattcctaa aattttttga aaccaaaggc cacaccgtcg tccgctcttc cagcctcgtg
     4561 ccgcacgacg acccgaccct gctgtttacc aacgcgggca tgaaccagtt taaagacgta
     4621 ttcttaggtt tcgacaaacg cccgtacagc cgcgccacca ccgcgcaaaa atgcgtacgc
     4681 gcaggcggca agcacaacga cttggaaaac gtcggctaca ccgcccgcca ccacaccttc
     4741 tttgaaatga tgggcaactt ctccttcggc gactacttca agcgcgacgc gatccacttc
     4801 gcttgggaat ttctgacttc ccccgaatgg ctcaatatcc ccaaagacaa actgttggcg
     4861 accgtttacg cggaagacga cgaagcctac aacatctggt tgaacgaaat cggtatgccg
     4921 tccgagcgca tcgtccgcat cggcgacaac aaaggcgcga aatacgcgtc cgacaacttc
     4981 tggcaaatgg gcgacaccgg cccttgcggc ccctgctccg aaattttcta cgaccacggc
     5041 gaagaaattt ggggcggcat tcccggcagc cccgaagaag acggcgaccg ctggatcgaa
     5101 atttggaact gcgtattcat gcagttcaac cgcgacgaac aaggcaatat gaatccgctg
     5161 cccaagccgt ccgtcgatac cggcatgggc ttggaacgca tggccgccgt tatgcagcat
     5221 gttcacagca actacgaaat cgacttgttc caagacctgc tcaaagccgt tgcccgcgaa
     5281 accggcgcag ctttcagcat ggaagaacca agcctaaaag tcatcgccga ccacatccgc
     5341 tcctgctcat tcctgattgc agacggcgta ttgccttcca acgaaggccg cggctacgta
     5401 ttgcgccgca ttatccgccg cgccgtgcgc cacggttaca aactgggtca aagcaaaccg
     5461 ttcttccaca aactggtggc cgatttggtc aaagagatgg gcgatgccta ccctgaattg
     5521 aaagaaaaac aagttcaaat cgaagaagcg ttgaaaaacg aagaaagccg ttttgcccaa
     5581 actctggaaa ccggtatggc tttgttggaa aacgcgcttg ccaaaggcag caaaaaactc
     5641 gacggcgaaa tcatcttcaa actctacgat acctacggtt tcccatacga cttgactgcc
     5701 gacatctgcc gcgaacgcaa tatcgaactg gacgaagcag gcttcgagcg cgaaatggaa
     5761 gcccaacgcg cacgcgcacg cgccgcacaa agcttcaaag ccaacgccca actgccttat
     5821 gacggtcaag acaccgagtt taaaggttat agcgaacgcc aaaccgaatc caaagtcctc
     5881 gccctctaca aagacggcga gcaagtcgac gaattgaacg aaggcgacag cggcgcagtc
     5941 gtcatcgact ttaccccgtt ctatgcagaa tccggcggcc aagtcggcga tgtcggctat
     6001 atcttctcag gcgaaaaccg ctttgaagta cgcgataccc aaaaaatcaa agcggccgta
     6061 ttcggtcaat tcggcgtaca aacttcaggc cgtctgaaag tcggcgacag cgttaccgcc
     6121 aaagtggacg acgaaatccg caatgccaat atgcgcaacc acagcgcaac ccacttgatg
     6181 cacaaagccc tgcgcgatgt attgggcaga cacgtcgaac aaaaaggctc tttggttacc
     6241 gccgaatcca cccgtttcga catttcccat ccccaagcgg taactgccga agaaatcgcc
     6301 gaagtcgaac gccgcgtcaa cgaagccgtt ttggcgaacg ttgccgtcaa cgccgccatt
     6361 atgagcatgg aagacgcgca aaaaaccggc gccatgatgc tcttcggcga aaaatatggc
     6421 gacgaagtac gcgtattgca aatgggcggt ttctctaccg aattgtgcgg cggcacgcac
     6481 gtttcacgca ccggcgacat cggcctcttc aaaatcatca gcgaaggcgg tattgccgca
     6541 ggcgtgcgcc gtatcgaagc catcaccggc ctgaacgcac tcaaatgggc gcaagagcaa
     6601 gagcgtttgg tgaaagacat cattgctgaa accaaagccc aaaccgaaaa agacgtactg
     6661 gcgaaaatcc aagcaggcgc ggcacacgcc aaagcattgg aaaaagaatt ggcacgcgcc
     6721 aaagccgaac tcgccgtcca cgcaggcgca aaactcttgg acgacgcaaa agacttgggc
     6781 gcagccaaac tcgttgccgc ccaaatcgaa gccgacgcag ccgccctgcg cgaaatcgta
     6841 accaacctga ccggaaaatc cgacaacgcc gtgattcttt tagcggcagt aaacgacggc
     6901 aaagtctccc tgtgcgccgg cgtatccaaa ccgttgaccg gcaaagtgaa agcaggcgat
     6961 ctggttaaat ttgcagccga acaagtcggc ggcaaaggag gcggccgtcc cgacttggcg
     7021 caggccggcg gcacggatgc cggcaaattg cccgaaatgc tggtaagcgt ggaaagctgg
     7081 ctttgtcaga aattaagtta aatttaaatc ttaataagcc atttgagtct tgttcaaatg
     7141 gcttatattt tggattgtta gaatgaatac taaaacagag ttacaaaaac ttttagaaga
     7201 ggatatatca actttaacag aaacactaat ttgtgcagat gctcttcctc caagatatgt
     7261 acgtagcatt gcaactccta ttgtccgtcg atggttaatt gacaaacaat taaatattct
     7321 cgcaaaagaa ataggtttaa ctatagaatt gcccatcttt gatacaagtt tggtatttga
     7381 aaaattgtca accttagaga ataaagttaa tttttatatg gctggtggag tttatttagg
     7441 cggagaattt attagctcta tatatcattc atcacaagaa ttttctggtg aaccaataat
     7501 ctatgctgaa ccaaatataa ttttatgtcc tgctgaaaaa tttttaactt taaaacgagt
     7561 tttccataat ggaaatattt ttaatatgaa tcagataatt acttttttat ccaataaaca
     7621 aggaggagta cattttgata aaaattacga taaatataaa acttggcaag ttgctataga
     7681 aaaagcagca aattttttaa aactcggtaa tccatataat gaagataaat tatctcttag
     7741 tgaagaacat gatactattt tagtagtttt accattagaa aaaggatatg aatggaattg
     7801 tcttgagata gaagtactat ccgctgctca atctctagca aatatctatt gcaacaaagt
     7861 tcgcctcata gatggtcatg tttggaagga ataaaagaaa aagcatagtt ggtaattttt
     7921 agttacgtag ggtgggcttc agcccaccgt tttttcattt ttgattgttg attggtgggc
     7981 taaagcccac cctacggtta ctaaaatccc gtcattacct cgcaggtggg aatccggttt
     8041 gttcggtttc ggttttttag ggacagtgcc gcaacattaa atttccagat tcccactttc
     8101 gcacaaataa cagcgcaaat agggcaaaga taaggatatg ggtttttttc tttgaattta
     8161 ctatacaatt cgcccgtccc cgttatagaa acccaagccg atgaaaaccc tgctccaact
     8221 ccaaaccgcc gcacaaaact tcgccgccta ctacaaagac caaaccgacg aacgccgcga
     8281 gaaagacacc ttctagaacg aatttttcgc cattttcggc atcgaccgca aaaacgtcgc
     8341 ccacttcgaa taccccgtca aagaccctgc cgacaacacc caattcgtcg atatattttg
     8401 ggaaggcatc ttccttgccg aacacaaatc cgccaacaaa aacctgacca aggccaaaga
     8461 gcaggcggaa cgttatttac aggaaatcgg gcgcaccaag ccctccgcgc tgcccgaata
     8521 ttacgccgtc agcgattttg cccatttcca cctttaccgc cgcgtacctg aagaaggcgc
     8581 agaaaaccaa tggcagttcc ctttggaaga attgcctgaa tacatcacgc gcggcgtttt
     8641 cgacttcatg ttcggcatcg aagccaaagt ccgccaaatt caagaagaag ccaacattca
     8701 agcggcggcg accatcggca ggctgcacga cgcgctcaaa gaagaaggca tttacgaaga
     8761 acacgagctg cgcctcttca tcacgcgcct gcttttcctc ttttttgccg acgacagcgc
     8821 cgttttccgg cgcaactacc ttttccaaga ctttttagaa aactgcaaag aagccgacac
     8881 gctcggcgac aagctcaatc aactctttga atttctcaac acacccgacc aaaagcgcag
     8941 caagacccaa agcgaaaaat ttaaaggttt cgaatacgtc aacggcggtc ttttcaaaga
     9001 acgcctgcgc actttcgact tcactgccaa gcagcaccgc gccttaatcg actgcggcaa
     9061 tttcgactgg cgcaacatca gtccagaaat cttcggcacg ctcttccaat ccgtcatgga
     9121 cgcgcaagag cggcgcgaag cgggcgcgca ctacaccgaa gccgccaata tcgacaaagt
     9181 catcaacggc ctttttttag aaaacctgcg tgccgaattt gaagccgtca aagccctcaa
     9241 acgcgacaaa gccaaaaaac tcgccgcctt ctaccaaaaa atccaaaacc tgcaattcct
     9301 cgaccctgcc tgcggctgcg gcaacttcct tatcgtcgcc tacgaccgca tccgcgccct
     9361 tgaagacgac atcatcgccg aagccctcaa agacaaagca gacggcctgt tcgacagccc
     9421 gtccgtccaa tgccgtctga aacagtttca cggcatcgaa atagacgaat ttgccgtcct
     9481 catcgcccgc accgccatgt ggctcaaaaa ccaccaatgc aacatccgca cacaaatccg
     9541 cttcgacggc gaagtcgcct gccatacgct gccgctcgaa gacgccgccg aaatcatcca
     9601 cgccaacagc ctccgcacac cttggcaggc ggcggactac atcttcggca atcccccctt
     9661 tatcggctcg acctaccaaa ccaaagagca gaaaaacgac ctcgaaagca tctgcggcca
     9721 tatcaaaggc tacggcctgt tggattacgt ctgcaactgg tacgtcaaag ccgcaggcat
     9781 catggcgcag catccccaag ttcagacggc atttgtttcc accaattcca tctgccaagg
     9841 ccagcaggtc gaaatcctct ggggcagcct tttaaaccaa ggcatcgaaa tccactttgc
     9901 ccaccgcacc ttccaatgga cgagccaagc cgcaggcaaa gccgccgtcc actgcatcat
     9961 cgtcggcttc cgccaaaagc cgccaatgcc gtctgaaaaa accctctacg actatcccga
    10021 catcaaaggc gaacccgaaa aacacgccgt agccaacatc aatccttatc tgatcgatgc
    10081 gcccgatttg attatcgcca agcgcagccg tcccatacat tgcgaacctg atatggtcaa
    10141 cggaagcaaa ccgaccgaag gcggcaacct tatcctttca accgccgaaa aagatgccct
    10201 gattgccgcc gaacccttgg cggagcaata catccgcccc tttatcggcg cggatgagtt
    10261 tctcaacggc aaaacccgtt ggtgcctgtg gtttcacggc gtatccgatg tcaaacgcaa
    10321 ccacgacctg aaacaaatgc cccaagttca agcccgtatt caggcggtca aaaccatgcg
    10381 cgaagccagc agcgacaaac aaactcaaaa agatgcagca accccgtggc tttttcaaaa
    10441 aatccgccag ccttcagacg gcaattatct gattattccg agcgtgtcgt ctgaaagccg
    10501 ccgtttcatc cccatcggtt atctgtcgtt tgaaacagtt gtcagcaatc tggcatttat
    10561 ccttccaaac gccaccctct accacttcgg catcctcagc tccaccatgc acaacgcctt
    10621 tatgcgtacc gttgcaggtc gtctgaaaag cgattatcgc tactctaata ccgtcgtgta
    10681 caacaacttc cccttccccg aaagctgccg gttgccgtct gaaaacgacc gccccgaccc
    10741 gctccgcgcc gccgtcgaag ccgccgccca aaccgtcctc gacgcgcgcg gacaataccg
    10801 ccgagaagcg caggaagccg gtttgcccga gccgaccctc gccgaactct atgcgcccga
    10861 cgcaggctat accgccctcg acaaagccca cgccaccctc gacaaggcag tcgataaagc
    10921 ctacggctac aaaacaggca aaaataccga cgacgaggca gaacgcgtcg ccttcctgtt
    10981 cgagctgtac cgcaaggcgg cggcaattgc gtagtagttg cgtagtagtt gcgtagtagt
    11041 tgcgtaggat agttgcgtag ggtgggcttc agcccaccaa ctcccaccaa ctcccaccaa
    11101 ctcccaccat cttcacccta aaaccccgtc atctccgcaa aagcaattgc gtagggtggg
    11161 cttcagccca ccgtttttct tcattttcgg ttgttgattg gcaggctgaa gcccaccctg
    11221 cggttacaaa aatcccgtca ttcccgcgca ggcgggaatc cagacctttc ggggcggaaa
    11281 cttatcggat aaaacgattt cccctgcatc ctggattccc actttcatgg gaatgacggg
    11341 atataggttt ccctacggac gtgttcagat tcccgccttc gcgggaatga cggcggagaa
    11401 gtttctgtct ttccgataac ttaccgtaac ttaaaatccc gtcattaccg caaaagcagt
    11461 tgctacggtt acaaaaatcc cgtcatcccc acgaaagcga aaatcctgcc tgtcggattt
    11521 cggttttttc gggcgtttcg ggaacttata aatcgtcatt cccgcgcagg cgggaatcca
    11581 gaacgcggga cggcggcaat attcaaaggt
//
LOCUS       DAWKOG010000051        11257 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.051, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000051 DAWKOG010000000
VERSION     DAWKOG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11257
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.051"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(127..1989)
                     /gene="hscA"
                     /locus_tag="WJK66_001015"
     CDS             complement(127..1989)
                     /gene="hscA"
                     /locus_tag="WJK66_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEZ7578740.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRVIELHTRQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCRLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMAIDTSISRAEFHNLTQHLVMKTLEPVTQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKTDGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDGAITRMLKDSMDNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAKELQQI
                     RQGIADLQGRLKDGKAEDIRSAVAKLSRSTDNFAAKRMNRNIQRALTGQSVDNI"
     gene            2160..3032
                     /gene="hpnD"
                     /locus_tag="WJK66_001016"
     CDS             2160..3032
                     /gene="hpnD"
                     /locus_tag="WJK66_001016"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEZ7578741.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKRDAVTVLYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVRARYGSFEELKLYCRRVAGVVGCLIARILGFSDGKTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMQRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPAADKKAQKVGLVMAAVYYELLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            3111..4424
                     /locus_tag="WJK66_001017"
     CDS             3111..4424
                     /locus_tag="WJK66_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ7578742.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARTLAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRAPTAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNPLPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTVQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFANRAWADKAHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPYYPATLEAAVQSGFASAEACLQSLNDAV"
     gene            4508..5227
                     /locus_tag="WJK66_001018"
     CDS             4508..5227
                     /locus_tag="WJK66_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ7578743.1"
                     /translation="MATLTDKTILVTGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGYPEPFAICFDLISAEEKEFEHFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASAESKGRSGEIVYL"
     gene            5392..6063
                     /locus_tag="WJK66_001019"
     CDS             5392..6063
                     /locus_tag="WJK66_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEZ7578744.1"
                     /translation="MKTVSTAVVLAAAAVSLPGCATESSRSLEVAKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLNALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            6073..6441
                     /locus_tag="WJK66_001020"
     CDS             6073..6441
                     /locus_tag="WJK66_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HEZ7578745.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGDNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            6438..7085
                     /locus_tag="WJK66_001021"
     CDS             6438..7085
                     /locus_tag="WJK66_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HEZ7578746.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
     gene            7374..8517
                     /locus_tag="WJK66_001022"
                     /pseudo
     CDS             7374..8517
                     /locus_tag="WJK66_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246189.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(8627..8836)
                     /locus_tag="WJK66_001023"
     CDS             complement(8627..8836)
                     /locus_tag="WJK66_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578747.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPTYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(8833..9129)
                     /locus_tag="WJK66_001024"
     CDS             complement(8833..9129)
                     /locus_tag="WJK66_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HEZ7578748.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRGARL
                     AQKPKTRWKVLYTLINGVFFDRHHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            complement(9108..9713)
                     /locus_tag="WJK66_001025"
     CDS             complement(9108..9713)
                     /locus_tag="WJK66_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578749.1"
                     /translation="MTIYFKNGFYDDTLGSIPEGAVAVRAEEYAALLAGQAQGGQIAA
                     DSDGRPVLTPPRPSEYHEWDGKKWEIGEAAAAARFAEQKTATAFRLAAKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTPMLAQIAAARGVELDVLIEKVIEKSARLA
                     VAAGAIIGKRQQLEDKLNAIETAPGLDALEKEIEEWTLNIG"
     gene            9793..10098
                     /locus_tag="WJK66_001026"
     CDS             9793..10098
                     /locus_tag="WJK66_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEZ7578750.1"
                     /translation="MKIEFDSEKNQRNIEERNLPFESVGQIRWTTAVIVPDVCFDYPE
                     PRYVAAAYLGDTQRLHIVCFTPIKDGIRVISFRKANSFRKANKREVKKYATSILNKR"
     gene            10070..10372
                     /locus_tag="WJK66_001027"
     CDS             10070..10372
                     /locus_tag="WJK66_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEZ7578751.1"
                     /translation="MQRQSLTNADGEVRELAAEDFALARPIAEALPEDLAQVLFSHQK
                     QLEEKGVMQKRNTGKTPKQLVTIRLSADVVEKFRAGGKGWQTRINEVLRQYVAQLK"
BASE COUNT         2415 a         3175 c         3112 g         2555 t
ORIGIN      
        1 acgcccttga tgacggtttt catcgaatag acaatgcctg acttttccat atcgatgttt
       61 ttcaagtgtt ttcgagcctt cagacggcat caggttatat ctatgccgtc tgaaaccgtt
      121 taagtatcaa atattatcga cactctggcc tgtcaatgcg cgttggatat tgcggttcat
      181 gcgtttggcg gcgaagttgt cggtgctgcg gctgagtttg gcgacggcgg agcggatgtc
      241 ttcggctttt ccgtctttca gacggccttg caaatcggcg atgccttgcc ggatctgttg
      301 caattctttg gcatccagta aatcgctgtc caactcgagg gcggcgttga cggcatcggt
      361 caggctttcg gcttcgacta cggcttcggc gcgggcgcgt gccgccatat cttcggcggc
      421 gttgtccata ctgtctttaa gcattcgggt aatcgcgccg tcgtccaagc cgtaggaagg
      481 tttgacttcg atttgcgcct gtacgccggt gctttgttct tgggcggaaa cggacagcag
      541 cccgtccgca tcgatttgga aggttacgcg gatacgcgcc gcacccgccg ccatcggtgg
      601 aatgccgcgc agggtgaatt tggcaaggct gcggcagtca gaaacgagct cgcgctcgcc
      661 ttgcacgacg tgtatcgtca tcgcggtttg accgtctttg aaggtggtaa agtcctgcgc
      721 gcgcgcggtg gggatggtgg aattgcgcgg gatgattttt tccgccaagc cgccgtaggt
      781 ttccaaaccg agcgacaaag gtgtaacgtc cagcagcagc cattcgccgt cggttttgtt
      841 gcctgcgagg acgtttgcct gtatggcggc gccgagcgcg acgacttcgt cgggattgag
      901 gttgttcagc ggggtttgtc cgaagaaggt ggcgactgcc tgttggacgt gcagcatacg
      961 ggtcgaaccg ccgaccataa tcacgccttt gacttcgttt ttaccgacac cggcatcttt
     1021 caacgcctgt gtgaccggtt cgagcgtttt catcaccaaa tgctgcgtca ggttgtggaa
     1081 ctcggcgcga ctgatgcttg tgtcgattgc catgccgtct gaaagcgtcg cctgaatgcg
     1141 cgcttcggtt tgcgtggtta attgttcttt ggcggcgcgg acgagcgaga gcaggagttg
     1201 gctgtcttgt tcgttgagtt gggagagtcc gttttgttcg agcaggcggc agaacaggcg
     1261 gtggtcgaaa tcgtcgccgc ccaacgcgct gttgccgccg gtggctttga cttcaaacag
     1321 tcctttggtc agttgcaata cggatacgtc gaatgtgccg ccccctaagt cgtacacgac
     1381 aaacgtgcct tccgaggcgt tgtccagccc gtaggcgatt gcggcagcgg tgggttcgtt
     1441 gagcaggcgc aatacgttca aacccgccag acgcgcggca tctttggtgg cttggcgttg
     1501 ggcatcatcg aaataggcag ggacggtaat caccacgccg accaaatcgc cgcccaaggt
     1561 ttcttcggcg cgcgatttaa gggttttgag gatttccgcc gacacttcga caggcgtttt
     1621 caccccctgc cgcgtatgca gttcgataac gcgttgattg tcgccgaaac ggtaaggcag
     1681 gtaatgcgta ttttgatgca gatcggcaag cgtccgcccg ataaggcgtt tggcggagct
     1741 gacggtgttc agcgggtcgg ttttttgggc ggacagggcg gttttgccga cttcaatgcc
     1801 gccgttttcc agatagcgga cgacggaagg cagggtaacg cgcccttcgg catcgggcag
     1861 acaggcggca ctgccgctgc ggacggtggc gaccaagctg ttggtcgtac ccaaatcgat
     1921 gcctgccgcc aaacggtgcc ggtgcggggc ggcggacata cccggttctg aaatctgcaa
     1981 aagagccata aattctgcct gtcggtgcgg tgtgtaaagg aaggaaagcg gcattttaac
     2041 agaatttgcg ccgatagttg agcaaaccgc taaggattgg gtcgtgccgc cgaaattgcc
     2101 ctctaccgcc aaatgccgat aaaatccgcc tttcccgccc atcattagga atataaatcg
     2161 tgaaaggact cgactattgc cgccaaaaag cagaagaaag ccgctccagt tttttgtcgg
     2221 gcttccgttt cctgactcag gaaaaacggg atgcggtaac ggttttatat gctttttgcc
     2281 gcgaattgga cgatgtggtt gacgaatgtt ccaaccccga tgttgcacag gcgacattga
     2341 actggtggcg cggcgatttg gacaaggtat tcggcggcgc gatgccggaa caccccgtca
     2401 atcaggcctt gcggcaggtt aaggaaacct tcaagctgcc gaaatatgaa ctggaagcct
     2461 taatcgacgg gatgcagatg gatttggttc gggcgcgtta cggcagtttt gaagaattga
     2521 aactgtactg ccgccgtgtt gcaggtgtag tcggctgcct gattgcgcgg attttggggt
     2581 tttcagacgg caaaacgctg gaatacgccg acaagatggg acttgcgctg caactgacca
     2641 acatcatccg cgatgtcggc gaagacgcgc gcaggggacg gatttacctg ccgatggagg
     2701 aaatgcagcg gtttgatgtc cccgcaagcg tgattttgca atgcagcccg acgggcaatt
     2761 ttgccgaatt gatggcgttc caaatcaaac gcgcccgtga aacctaccgc gaagccgtat
     2821 cgctgctgcc ggctgccgat aaaaaagccc aaaaagtcgg actggttatg gcggcggttt
     2881 attacgagct attgaacgaa atcgaccggg acggcgcgca aaacgtcctc aaatacaaaa
     2941 tcgccctccc ttcgccgcgc aaaaaacgca ttgccctgaa aacctggtta ttcggattca
     3001 aaccgcgccc cggcacgccg gaacgggcat aaggcgcata ccgcccgccc ttcaggaaaa
     3061 cccaaccccc gccgccccga aaaacggggt ttcaaccatt ggaggaataa atgatgaaca
     3121 cgccgcatcc gcgcccgaaa atcgccgtca tcggcgcagg ctgggcagga ctgtccgccg
     3181 ccgtcacctt ggcgcggcac gccgacgtta ccctgtttga agccggccgg caggcgggcg
     3241 gcagggcgcg cacactggcc ggaaataccg acggtttcgg ttttttggac aacgggcagc
     3301 acattttgct cggcgcatac cggggcgtgt tgcgcctgat gaaaaccatc ggttcggatc
     3361 cccgtgccgc ctttttgcgc gtaccgctgc actggcatat gcacggcggt ttgcagttcc
     3421 gcgccctccc cctgcccgcg ccgctgcata ttttgggcgg cgtgctgctt gcccggcgtg
     3481 caccgactgc attcaaagcc aaactgcttg ccgatatgtc cgatttgcag aagtccgcac
     3541 gcctcggaca gcccgacacg acagttgcac aatggctgaa acagcggaac gtgccgcgtg
     3601 ccgccgtgat gcagttttgg cagccgctgg tgtggggtgc gctcaacacg cctttggaaa
     3661 ccgcaagcct gcgcgtgttg tgcaacgttt tgtccgacgg cgtgctgacg aaaaaatccg
     3721 gcagcgacta tctcctaccc aagcaggatt tgggcgcaat cgtcgccgaa cccgccttgg
     3781 cggagcttca acggctcggc gcggacatcc gcctcgaaac gcgcgtatgc cgtctgaacc
     3841 ccctcccgga cgggaaagtg ctcgtcaacg gcgaagcttt cgatgccgcc gtccccgcca
     3901 ccgcgcccta ccacgccgcc gcgctcctgc ccgaaggcac gcccgaacac gttcagacgg
     3961 catatcaaaa ccttcgctat cacgccatca ccaccgtcta tctgcgctac gccgaacccg
     4021 tccgcctgcc cgccccgctg accggccttg ccgacggcac ggtgcaatgg ctgctttgcc
     4081 ggggcaggct cggactgcct gaaaacgaag tgtccgccgt catcagcgtt tccgaccgcg
     4141 tcggcgcgtt tgcaaaccgg gcgtgggcgg acaaagccca cgccgacctc aaacgcatcc
     4201 ttccgcattt gggcgaaccc gaagccgtgc gcgtcatcac cgaaaaacgc gccacaaccg
     4261 cagccgatgc cccaccaccg gatttgtcgt ggctgcaccg gcaccgcatc ttccccgccg
     4321 gcgactacct ccacccatac taccccgcca cgctcgaagc cgccgtacaa tcaggtttcg
     4381 cttccgcgga agcctgcctg caaagcctga acgatgccgt ctgaaaacgc cggccggcat
     4441 caggacgctt tacaaggtgc ggcaaaacgc taaaataccc ccgaccgacc accaaaaagg
     4501 aaaaagtatg gcgacactga ccgacaaaac catcttagtt accggcgcat cgcaagggct
     4561 gggcgaacag gttgccaaag cctatgcggc agcaggcgcg actgtgattt tggttgcccg
     4621 tcatcagaaa aaactggaaa aagtgtatga cgcgattgtc gaagccggat accccgaacc
     4681 attcgccatc tgctttgacc ttattagcgc ggaagaaaaa gaatttgaac atttcgccgc
     4741 caccattgcc gaagccacgc aaggcaaact ggacggcatc gtccactgcg ccggctattt
     4801 ttacgccctc tcgccgctgg atttccaaac cgtcgccgaa tgggtcaacc aataccgcat
     4861 caacaccgtc gcacctatgg ggctgacccg cgccctgttc ccgctgctga agcagtcgcc
     4921 cgacgcgtcc gtcatcttcg tcggcgaaag ccacggcgaa acacccaaag cctactgggg
     4981 cggcttcggc gcgtccaaag ccgcgttgaa ctacctgtgc aaagtcgccg ccgacgaatg
     5041 ggaacgcttc ggcaacctgc gcgccaacgt cctcgtcccc ggccccatca attccccgca
     5101 acgcatcaaa tcccatccgg gcgaagccaa aagcgaacgc aaaagctacg gggacgtgct
     5161 gcccgcattt gtctggtggg caagtgccga aagcaaaggg cggagtggcg aaatcgttta
     5221 cctctaaatc cggaaaaggc aggcatcccg cccgcgccgc gattccgttt gaaaaatatc
     5281 cgtaccttcc gcgcgcatcc gtcccgcaca gacaaacacc ttgacttaaa tttacataag
     5341 gacaataatg ataactcgtt atttccttaa cttgacctta gggtaaattt tatgaaaacc
     5401 gtttccaccg ccgttgtcct tgccgccgct gccgtttcac tgcccggctg tgcgaccgaa
     5461 tcctcacgca gtctcgaggt agcaaaagtc gcctcctaca atacgcaata tcacggtgtt
     5521 cgtaccccga tttccgtcgg aacattcgac aaccgctcca gcttccaaaa aggcattttc
     5581 tccgacgggg aagaccgttt gggcagccag gcaaaaacca ttctggtaac gcacctgcaa
     5641 cagaccaacc gcttcaacgt actgaaccgc accaatttga acgcattaaa acaggaatcc
     5701 ggcatttccg gcaaagcgca taacctgaaa ggcgcagatt atgtcgttac cggcgatgta
     5761 accgaattcg gacgcagaga tgtcggcgat catcagctct tcggcatttt gggtcgcggc
     5821 aaatcgcaaa tcgcctatgc aaaagtggct ctgaatatcg tcaacgtcaa tacttccgaa
     5881 atcgtctatt ccgcacaggg cgcgggcgaa tacgcacttt ccaaccgtga aatcatcggt
     5941 ttcggcggca cttccggcta cgatgcgact ttgaacggca aagttttaga cttggcaatc
     6001 cgcgaagccg tcaacagcct ggttcaggct gttgacaacg gcgcatggca acccaaccgt
     6061 taaggagaaa tgatgaatcc gaaaaccttg agccgtttgt cgctgtgtgc ggcggtcttg
     6121 gctctgaccg cctgcggcga caacgggcaa aaatccctgt attattacgg cggctatccc
     6181 gataccgtct atgaaggttt gaaaaacgac gacacttcgt tgggcaagca gaccgaaaag
     6241 atggaaaaat actttgtgga agccggcaac aaaaaaatga atgccgcccc gggtgcgcac
     6301 gcccatctgg gactgctgct ttcccgttcg ggagacaaag agggcgcgtt ccgccagttt
     6361 gaagaagaga aaaggctgtt tcccgaatcg ggcgtattta tggacttcct gatgaaaacc
     6421 ggtaaaggag gcaagcgatg aaacctttga ttttagggct tgccgccgtg ttggcgctgt
     6481 ctgcctgcca agttcaaaaa gctcccgatt tcgactacac gtcattcaag gaaagcaaac
     6541 cggcttcaat tttggtggtt ccgccgctga acgaatcgcc cgatgtcaac ggaacatggg
     6601 gtgtactggc ttcgaccgcc gcgccgcttt ccgaagccgg ctattacgtc ttccccgccg
     6661 cagtcgtgga ggaaaccttc aaacaaaacg gcttgaccaa tgccgccgat attcacgccg
     6721 tccggccgga aaaactgcat cagattttcg gcaatgatgc ggttttgtac attacggtta
     6781 ccgaatacgg cacttcatat caaattttag acagcgtgac gaccgtatcc gccaaagcac
     6841 ggctggtcga ttcccgcaac ggaaaagagt tgtggtcggg ttcggccagc atccgcgaag
     6901 gcagcaacaa cagcaacagc ggcctgttgg gggctttggt cagcgcagtg gtcaatcaga
     6961 ttgccaacag cctgaccgac cgcggttatc aggtttccaa aaccgccgca tacaacctgc
     7021 tgtcgcccta ttctcacaac ggcatcttga aaggtccgag attcgtcgaa gagcagccca
     7081 aataagcata tgccgtctga acctctccga gcttcagacg gcatatgcga atcttgatac
     7141 ttgcaagccg caccgcccaa aatcgggcgg ccggtttcaa tatccgaaca aggttcgggt
     7201 tctgtatttt gcagaacccg aaccttttat tcaaatttaa acttaattcc ggcgtggttg
     7261 tatggtaaat taacacgcaa tatgccctga cctgcaaaac ttccccaaac ttcatcaaaa
     7321 aatcaactgt acccgtcttt ttcagacggc attgataccg taagaaattt cccatgcaga
     7381 aatggcaaac cgagctttat tccacaccgc tttggctttg tctgacactg aaaagcgcgg
     7441 tcaaagtctt gctgctgatt acggcgatga ttacgctggt gttaaccgaa atgcggctga
     7501 atgtgttgag tacctttatg tccaaagggc tttacgactc gatgcaggat ttgaaatgct
     7561 tcggcatttt ggatgtttgc tgcaatgaac gcgggcgtgg tgctgatacg ggcgtttaac
     7621 aacgtcgtca acgacttcct cgatcaaggc ttggcgatta aatggtcgga gcggctcaac
     7681 gaagtgctga cagcacgctg gcttgccgac aaaaactact accgcctgca aatgcgccgc
     7741 cacgcgccag acaacatcga ccagcgtatc caacaggacg cgcaggaatt tatcgcttcg
     7801 accatcgaat ttgtgcgcgg catggtcaat tcggtcgtta cttctctgga atttgccgtt
     7861 gttttgcggg gcttggctgg catcctgacc gtgttcggct tcgacattcc gcacggcatc
     7921 gtttggtttg tctatatatt tgtgattttg gcgaccttta tcgccatgtg gataggcaac
     7981 cctttgattc gttacaatta tgaaaacgaa aaactcaacg gcgactaccg ttattccctc
     8041 atccgcgtgc gcgaccacgc cgaaagcgtg gcgttttaca acggcgaaaa acatgaatac
     8101 gaccagcttt ccgaccgctt taaagccatt atccgcaacc gttggcgcat tgcgcggcaa
     8161 agcgtctgct taagcggctt taacgatatg ttcaccaacg gcatcaaact cttcccgatt
     8221 attttgcaag ctccgcgcct gtttgccgga caaatcaaaa tcggcgacat ccagcaaacc
     8281 gtccaagcct tcgcgcgact gcaaaacgcg ctttctttct ttcgaatgtt ctacaacaaa
     8341 ttcaccgcct accgcgcccg attggagcgt ctgtacggct ttttgctgag taccgaagaa
     8401 caacacagcg cgcggcaacc cgagattagc gaagtttcag acggcattgc actggaatgt
     8461 aaggcggcgg cagcttttgc acgaattggc ggcagctttt gcaccgccga agcgtaggaa
     8521 attgtattag cttttgcaca aatttatccc cgtttaatgg tctgttaaac ggggattatc
     8581 ttatgccttt aaaagtagta cgctacttgc ggtcagaact tataaatcag gtggtcgtcg
     8641 tagcttgaat tgctgttgat gtgctgctca cagtcaaggt ttggctgcct gcaaaacgct
     8701 cccatccggt caggcgcagt tctgccacga ggtcaagata agtaggcagt tcgctgcggg
     8761 tgccggagaa gaagacaaat ggcggccgca ccattgccat caggcgcaga aactctacca
     8821 tgccgaagta gttcatcgcg tccccttttc cccggcggcg cggctttggt tgaaccgcgc
     8881 gtcgtgctgc ctgcctttca gttcgctgat atacgcctgc cggcagtggt ggcggtcgaa
     8941 aaatacgccg ttgatcaggg tgtataaaac cttccagcgg gttttgggct tttgggcgag
     9001 ccgcgcgccg cgataggtgc ggcttgagag ggtttcgtcc gccgcgccgc cggttagggc
     9061 gttgaacagt tggtcgatgg ctaccaacag gtggtaaacg tattttttca gccgatgttt
     9121 agcgtccatt cttcgatttc cttttccagc gcgtccaatc ctggcgcggt ttcgatggcg
     9181 ttcaatttgt cttcgagctg ctgacgcttt ccgataatcg cgccggcggc aacagccagg
     9241 cgggcggatt tttcgataac tttttcaatc aaaacgtcca attccacgcc ccttgcggcg
     9301 gcgatttgcg ccagcatcgg ggtcggggcg ttgttgtccg cctgccgcgc gagggcttct
     9361 ttttcctgcc tgtaaaagct gtcgatttcc acttggggat agcccgccaa gaggctgttt
     9421 ttgagttcgt ccgcctttgc cgcgaggcgg aatgccgtgg cggttttttg ttcggcgaaa
     9481 cgggcggcgg cagcggcttc gccgatttcc catttcttgc cgtcccattc gtggtattcg
     9541 gacgggcgcg gcggggttaa aacggggcgg ccgtcggaat ctgcggcaat ctgcccgccc
     9601 tgcgcctgtc ctgccaaaag ggcggcgtat tcttcggcgc ggacggcaac cgcgccttcg
     9661 gggatgctgc ccaaggtgtc gtcgtaaaag ccgtttttga aatagatggt catttcgggg
     9721 tttccttttt tgtatttaca ttttggtttt ttgccgctta taatgtaacg acaaaatacg
     9781 ctgcaagctg caatgaagat tgaatttgac tccgaaaaaa atcaacgcaa tatcgaagag
     9841 cggaatttgc cctttgaatc agtcgggcaa atacgctgga caacggccgt tattgtgccc
     9901 gatgtctgct ttgattaccc cgaaccgcgt tatgttgccg ctgcttatct aggcgatacg
     9961 caacgcctgc acattgtctg ttttacgccg ataaaagacg gcatccgtgt catcagtttt
    10021 cgcaaagcga atagttttcg caaagcgaat aaaagagagg ttaaaaaata tgcaacgtca
    10081 atccttaaca aacgctgacg gcgaagtgcg ggaacttgcc gccgaagatt ttgcgcttgc
    10141 ccgccccatt gccgaagccc tgcccgaaga cttggcacag gtgctttttt cccatcaaaa
    10201 acaattggaa gaaaagggcg taatgcaaaa acgaaacaca ggcaaaaccc cgaaacaact
    10261 cgttaccata cgcctgtccg ccgatgtggt cgaaaaattc cgcgccggcg gcaagggctg
    10321 gcagacgcgc atcaacgagg ttttgcgcca atatgtcgcc caattgaaat aacgggggcg
    10381 gccgctgctt atcgggcgcg cccgactatc ctttcccgcg cggtttcggc cgtgcggttt
    10441 tttgttgccg gtgccgtccg aagggggcag cggctttttg cgcggtcatt cccgcctttc
    10501 gttattcccg ccactttccg tcattcccgc gaaggcggga atcccgaaac ccctgccgcc
    10561 gtcattcccg cgcaggcggg aatccagtcc gttcggtttc agccgtttcc gatagattcc
    10621 tgccgcgttt ggggtctgga ttcccgcctg cgcgggaatg acggcggcgg ggggtcggtt
    10681 ttttccgata gattcctgcc gcgtttgggg tctggattcc cgcctgcgcg ggaatgacgg
    10741 cggcgggggt ttctgttttt tccgataaat tcctgccgcg tttggggtct ggattcccgc
    10801 ctgcgcggga atgacggcgg cgggggtttc tgttttttcc gatagattcc tgttgcgtcg
    10861 gggtctggat tcccgctttc gcgggaatga cggcggcggg ggtttctgtt ttttccgata
    10921 gattcctgcc gcgtttgggg tctagattcc cgctttcgcg ggaatgacgg tggcgggggt
    10981 ttctgttttt tccgatagat tcctgccgcg tttaggggct ggattcccgc tttcgcggga
    11041 atgacgaaag cggcgggaat gacgaagagt ggcaggattg gcggggcata ggtttcgggg
    11101 gattggcggc gcggccggtt tcggtcggat tgcttggatc ggcgggctga agcccatctc
    11161 tacaactcgt ctctacaacc catctctaca acccatctct acaacccatc tctacaaccc
    11221 atctctacaa cccatctcta caacccatct ctacagc
//
LOCUS       DAWKOG010000052        11111 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.052, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000052 DAWKOG010000000
VERSION     DAWKOG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11111
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.052"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..123
                     /locus_tag="WJK66_001028"
     CDS             <1..123
                     /locus_tag="WJK66_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEZ7578752.1"
                     /translation="FYADPSYTKAQIGWQTQRDLSQMMEDSWRWVSNNPNGYDD"
     gene            239..1264
                     /gene="rfbB"
                     /locus_tag="WJK66_001029"
     CDS             239..1264
                     /gene="rfbB"
                     /locus_tag="WJK66_001029"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEZ7578753.1"
                     /translation="MRKILVTGGAGFIGSAVVRHIIRNTQDSVVNLDKLTYAGNLESL
                     TDIADNPRYAFEQVDICDRAELDRVFAQHRPDAVMHLAAESHVDRSIGSAGEFIQTNI
                     VGTFNLLEAARAYRQQMPSEKHEAFRFHHISTDEVYGDLSGTDDLFTETAPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYG
                     DGMQIRDWLFVEDHARALYQVVTEGVVGETYNIGGHNEKANIEVVKTICALLEELAPE
                     KPAGVARYEDLITFVQDRPGHDARYAVDTAKIRRDLGWQPLETFESGLRKTVQWYLDN
                     KTRRQNA"
     gene            1370..2236
                     /gene="rfbA"
                     /locus_tag="WJK66_001030"
     CDS             1370..2236
                     /gene="rfbA"
                     /locus_tag="WJK66_001030"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEZ7578754.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENFRAVSIEEK
                     PQRPKSDWAVTGLYFYDNRAVEFAKQLKPSARGELEISDLNRMYLEDGSLSVQILGRG
                     FAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLSDEKLEELARPMAKN
                     QYGQYLLRLLKK"
     gene            2290..2829
                     /gene="rfbC"
                     /locus_tag="WJK66_001031"
     CDS             2290..2829
                     /gene="rfbC"
                     /locus_tag="WJK66_001031"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ7578755.1"
                     /translation="MNIIDTAIPDVKLLEPQVFGDARGFFMETFRDEWFKTQVCERTF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMREGSPTFGKWAGATLS
                     AQNQCQLWIPEGFAHGFCVLGDAAEVVYKCTDYYKPEAEQVLIWNDPTVGIDWPLQTA
                     PLLSPKDLAGKVLAEAVTF"
     gene            2853..4967
                     /locus_tag="WJK66_001032"
     CDS             2853..4967
                     /locus_tag="WJK66_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HEZ7578756.1"
                     /translation="MFLFSDGLQSINNNNRRKRIVKNAYIPSRGIRKIPYLSTLLPEF
                     HICKDGKGAEAVIGWGLRPTTHKARAFATEHQLPFIALEDGFLRSLGLGVSGYPPYSI
                     VYDDIGIYYDTTRPSRLEQLILAADTMPSETLAQAQQTMDFILQHHLSKYNHAPELSD
                     DHPLRSPSKPETVLIIDQTFGDMAIQYGGADASTFELMFQTALNENPQADLWVKTHPD
                     VLCGKKQGYLTQLAQQHRVHLLAEDINPISLLQAVDKVYCVTSQMGFEALLCGKPLVT
                     FGLPWYAGWGVSDDRHPEINRLVQTQRRATRNLLQLFAAAYLQYSRYLNPNTGEAGSL
                     FDVIDYLATVKRKNDKLRGELYCVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLARVK
                     LSDDARILAWGNGKEAIVRFAEQHHIPLLRMEDGFIRSVGLGSNLVPPLSLVTDDMSI
                     YFNAETPSRLEYILQNQNFDDQDFQTALKLQKMLTENHISKYNVGSSDFTAPSTDKTV
                     ILVPGQVEDDASIRYGSPQIYRNLDLLRTVRERNPNAYIIYKPHPDVVSGNRIGHISP
                     EDAARYADQTAEQTDILTCLQYADEVHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLT
                     QDLLPIPRRSRRLELWQLIAGTLIHYPDYIHPETHQAINAETAAQILIRQKNMQKNNN
                     GLHRGYFAKKLGKIKQLYRSFK"
     gene            5104..6363
                     /locus_tag="WJK66_001033"
     CDS             5104..6363
                     /locus_tag="WJK66_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide modification protein
                     LipB"
                     /protein_id="HEZ7578757.1"
                     /translation="MKQTVLKNNLQNLLESAENILLLQGPVGDFFLRLADWLTANGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYITQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVHGGFTPMAKNAIRYYIELFRNPRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLSSFAEHAP
                     ADTNIIIKHHPMDRGFIDYWRDIKRFIKEHPELKGRVIYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAYYDIPGITDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKKTSNSSTPVI"
     gene            6731..8137
                     /locus_tag="WJK66_001034"
     CDS             6731..8137
                     /locus_tag="WJK66_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ7578758.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYTEAAFVLG
                     NHLLKNGHPEQALPWLEAAAAQRHPKALFTLLQQREHNGTPTGQLLNEYAWLSEQGHL
                     EAQLILMRYHTQRNDPQSLYWAELAAAQYAAPAYYHLARHHQRQGDVATAIEQYEKAA
                     ALGITAACWQLGQIYFYGTGVSPNHAQAEHYLEQAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAANKEQAEAQAKLAQYALTGELSERDPFQAARYAKAAAEKDHPEALKIMGD
                     LYRYGLGIKADNHIAHDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAEAQACAIGRPADYNAARKNYMEAAGFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAIGNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            8355..9569
                     /gene="gltS"
                     /locus_tag="WJK66_001035"
     CDS             8355..9569
                     /gene="gltS"
                     /locus_tag="WJK66_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEZ7578759.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     IAAIVLFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            9668..10684
                     /locus_tag="WJK66_001036"
     CDS             9668..10684
                     /locus_tag="WJK66_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ7578760.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSVALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDADVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            10695..10892
                     /locus_tag="WJK66_001037"
     CDS             10695..10892
                     /locus_tag="WJK66_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578761.1"
                     /translation="MNTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAV
                     RLGENGFTEEQMENIANLFGR"
     gene            10910..11059
                     /locus_tag="WJK66_001038"
     CDS             10910..11059
                     /locus_tag="WJK66_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578762.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFSCVAYADVSTHRTIQKTS
                     PYVPS"
BASE COUNT         2834 a         3199 c         2614 g         2464 t
ORIGIN      
        1 ttctatgctg acccttctta tacgaaggca caaatcggtt ggcaaactca gcgtgattta
       61 agccaaatga tggaagactc atggcgctgg gtgagtaata atccgaatgg ctacgacgat
      121 taagttgacc tgatacaggc cgtctgaaag agatgttttc agacggcctc tttatctgaa
      181 aaacacacat tctgtctgct ataatctgtt tatatttttt ggctatcctc tgaaatttat
      241 gagaaaaatc cttgttaccg gcggtgcggg ctttatcggc tctgccgttg tccgtcatat
      301 tatccgaaac acccaagatt ccgtcgtcaa cctcgacaaa ctgacctacg cgggcaatct
      361 cgaatcgctg accgacattg ccgataatcc ccgctatgct ttcgagcaag tggacatttg
      421 cgaccgcgcc gaactcgacc gcgtattcgc acaacaccgg cccgatgccg tgatgcactt
      481 ggcggcggaa agccatgtcg accgctctat cggttcggca ggcgagttta tccaaaccaa
      541 tatcgtcggc acattcaacc tacttgaagc tgcgcgtgcc taccggcagc aaatgccgtc
      601 tgaaaagcac gaagccttcc gtttccacca tatttctacc gatgaagttt atggcgattt
      661 aagcggcaca gacgatttgt ttaccgaaac cgcgccctac gcgccgtcca gcccttactc
      721 tgcctctaaa gcgtccagcg accacctcgt ccgcgcgtgg ttgcgtactt acggcttgcc
      781 gaccattgta accaactgct ccaacaacta cggtccttac cattttccgg aaaaactcat
      841 tcctttgatg attctgaacg cgcttgacgg caaaccgctg cctgtgtacg gcgacggtat
      901 gcaaatccgc gactggctgt ttgtcgaaga ccacgcgcgc gcactgtatc aggttgttac
      961 cgaaggtgtt gtcggcgaaa cctacaatat cggcggccac aatgaaaaag ccaatattga
     1021 agtcgtcaaa accatctgcg ccctgctgga agaactcgct cccgaaaaac cggccggcgt
     1081 ggcgcgttat gaagatttga ttactttcgt acaagaccgc cctggccacg atgcccgcta
     1141 cgccgtcgat acggcgaaaa tcagacggga tttgggctgg cagcctttgg aaacctttga
     1201 atccggcctc cgcaaaacag tgcaatggta cttggacaac aaaactcggc ggcaaaacgc
     1261 ataaaacggc agctatcgtt tggaacgttt aggcaccgga aaataagttt tcagacggca
     1321 tcccgacgca atgccatctg aaaacccatc gcaaaggaag aaagaaaaga tgaaaggcat
     1381 catactggca ggcggcagcg gcacgcgcct ctaccccatc acgcgcggcg tatccaaaca
     1441 gctcctgccc gtgtacgaca aaccgatgat ttattacccc ttgtcggttt tgatgctggc
     1501 gggaatccgc gatattttgg tgattaccgc gcctgaagac aacgcctctt tcaaacgcct
     1561 gcttggcgac ggcagcgatt tcggcatttc catcagttat gccgtgcaac ccagtccgga
     1621 cggcttggca caggcattta tcatcggcga agaatttatc ggcaacgaca atgtttgctt
     1681 ggttttgggc gacaatattt tttacggtca gtcgtttacg caaacattga aacaggcggc
     1741 agcgcaaacg cacggcgcaa ccgtgtttgc ttatcaggtc aaaaaccccg aacgtttcgg
     1801 cgtggttgaa tttaacgaaa acttccgcgc cgtttccatc gaagaaaaac cgcaacggcc
     1861 caaatccgat tgggcggtaa ccggcttgta tttctacgac aaccgcgccg tcgagttcgc
     1921 caaacagctc aaaccgtccg cacgcggcga attggaaatt tccgacctca accggatgta
     1981 tttggaagac ggctcgctct ccgttcaaat attgggacgc ggtttcgcgt ggctggacac
     2041 cggtacgcac gaaagcctgc acgaggcggc ttcgttcgtc caaaccgtgc aaaacatcca
     2101 aaacctgcac atcgcctgcc tggaagaaat cgcttggcgc aacggttggc tttccgatga
     2161 aaaactggaa gaattggcgc gcccgatggc gaaaaaccaa tacggccaat atttgctgcg
     2221 cctgttgaaa aaataatgtt tgaggccgtc tgaaactttt cagacggcct ttagatgaaa
     2281 gataaaaaga tgaacatcat tgataccgcc attcctgacg taaaactgct tgagccccaa
     2341 gtcttcggcg acgcgcgcgg cttttttatg gaaaccttcc gcgacgagtg gtttaaaacc
     2401 caagtctgcg aacgcacctt cgtgcaggaa aaccactcca aatccggcaa aggcgtattg
     2461 cgcggcctgc actaccaaac cgaaaacaca caaggcaaac tcgtacgcgt agttgtcggc
     2521 gaagtgttcg acgtggccgt cgatatgcgc gaaggttcgc cgactttcgg caaatgggcg
     2581 ggcgcaaccc tgtccgcgca aaaccaatgc cagctttgga tacccgaagg cttcgcgcac
     2641 gggttttgcg ttttgggcga cgcggcggaa gtcgtgtaca aatgcaccga ctattacaag
     2701 cctgaagccg aacaggtttt aatatggaac gacccgacag tcggcatcga ctggccgctt
     2761 caaaccgcgc cgctgctgtc gcctaaagac ttggcaggca aagtattggc cgaagcagtc
     2821 acgttttaag actattcagg ccgtctgaaa gaatgttcct cttttcagac ggcctacaat
     2881 ccattaataa caataatcga cgaaaacgca ttgtgaaaaa cgcctacatc ccctctcgcg
     2941 gcatccgcaa aatcccctat ctctccactc tattgcctga gtttcatatc tgcaaggatg
     3001 ggaaaggagc agaggctgtt atcggctggg gtttgcgccc gacgacacac aaagcgcgtg
     3061 cttttgccac cgaacaccag cttcccttta ttgctttgga agacggcttt ttacgctcgc
     3121 tcggactggg cgtctccggt tatcccccct actccatcgt ctatgacgac atcggcatct
     3181 actacgacac tacccgccct tctcgtttgg aacaactgat tcttgccgcc gataccatgc
     3241 cgtctgaaac cttggctcag gcgcaacaga cgatggattt catcctgcaa caccacctgt
     3301 ccaaatacaa ccacgcgccc gaactttcag acgatcatcc tttacgctcc ccatccaagc
     3361 ctgaaactgt cctcattatc gaccaaacct tcggcgatat ggccatccaa tatggcggcg
     3421 cagacgcctc tacgtttgaa ctgatgtttc agacggcctt aaatgaaaac ccgcaagccg
     3481 atctttgggt caaaacccac cccgatgttt tgtgcggcaa aaaacaaggc tatctgaccc
     3541 agctggcgca gcaacaccgc gtccatcttt tggctgaaga cattaatccg atttcgctgc
     3601 tgcaagcggt ggacaaagtg tattgcgtga cctcgcaaat gggttttgaa gccttgttgt
     3661 gcggcaagcc tttggtaacg tttggtttgc cgtggtatgc cggctggggt gtaagcgacg
     3721 accgccatcc tgaaatcaac cgccttgttc aaacccaacg ccgcgccacc cgcaacttgc
     3781 tgcagctctt cgccgcagcc tatctgcaat acagccgcta cctcaacccc aataccggcg
     3841 aagcaggcag cctctttgat gtcatcgact atctggcgac ggtcaaacgt aaaaacgaca
     3901 aattgcgtgg cgagttatat tgcgtcggta tgtctttgtg gaaacgcgcg gttgccaaac
     3961 cgttctttaa cgtaccctct tgccgtctga aatttatctc ttccacccaa aaactggcaa
     4021 gggtcaaact gtccgacgat gcacgcatcc tggcttgggg caacggcaaa gaggccatcg
     4081 tccgctttgc cgaacaacac cacatccccc tgctgcgcat ggaagacggc tttatccgct
     4141 cggtcggact cggctccaac ttagtgccgc cgctgtcgct cgttaccgac gatatgagca
     4201 tttatttcaa tgccgaaacc ccgtcccgtc ttgaatacat cctacaaaac caaaacttcg
     4261 acgatcaaga ctttcagacg gccttgaagc tgcaaaaaat gctgaccgaa aaccacatca
     4321 gtaaatacaa cgtcggcagc tcagacttca ccgccccgtc aaccgacaaa accgtgatcc
     4381 tcgttcccgg ccaggttgaa gatgatgcgt ctatccgcta cggttcgccc caaatctacc
     4441 gcaatctgga tttgctccgt accgtacgcg aacgaaaccc caatgcctat atcatctaca
     4501 aaccgcatcc cgatgtagtc agcggtaacc gcatcggcca tatttcccct gaagatgctg
     4561 cacgatatgc cgaccaaacc gccgagcaaa ccgacatcct gacctgtctc caatacgcag
     4621 acgaagtaca taccatgact tcgctgaccg gttttgaagc cttgttgcgc ggcaaaaaag
     4681 tcagctgcta cggcctgcct ttttacgcag gctgggggct tacccaagat ctgctcccca
     4741 tcccgcgccg tagccgcaga cttgagcttt ggcagctgat tgccggcacg ctcatccact
     4801 atcccgacta catccacccc gaaacccatc aggccataaa tgcagaaacc gcagcccaaa
     4861 tcctgatacg acaaaaaaat atgcaaaaaa acaacaacgg attacatcgc gggtactttg
     4921 ccaaaaaatt aggtaaaatc aaacaactat atcgatcttt caaataaata ccatcaaagt
     4981 taacgatgcg tcataaactt gcctctattg cggcatcatt gcctttgcat cgttaattct
     5041 cttggcgtat gcttgaaagt tcaacctaaa actattacat aaaaaacaaa accacattgc
     5101 aacatgaaac agaccgtcct caaaaataac ctgcaaaacc tgcttgaaag cgcagaaaat
     5161 atcctgctgc ttcaaggccc tgtcggcgat ttttttctgc gccttgccga ctggctgact
     5221 gcaaacggca aaaccgtaca taaattcaac tttaatgcag gcgacgacta tttttatccg
     5281 cccactcaag cgcataccgt tgtttttaac gacaactacg atgcctttcc tgagtttttg
     5341 caagaataca tcactcaaca tcacatccag gccgttgtct gctttggcga cacacgccct
     5401 tatcacgtca ttgcaaaacg cattgcaaac gaaaaccaag ccagtttctg ggcgtttgaa
     5461 gaaggctatt tccgccccta ctacatcacc ttagaaaaag acggcgtcaa cgcattttcc
     5521 ccgttgccgc gccgtgccga cttttttctt gaacaattcc ctaagcttgc ccagcaagaa
     5581 tataaagcgc caacgccggt acacggcggt tttacgccca tggcaaaaaa cgctatccgt
     5641 tactatatcg agttgttccg caatccacgc aaataccccg actacatcca ccaccgcgca
     5701 cccaatgccg gccattacct caaaccgtgg tcgctctcca tcctcaagcg tttgaactac
     5761 tatattgaag acatccaaat cgccaaacgt gtggaagcag gcaaatacgg caagtttttt
     5821 attgttccct tacaggtatt caacgacagc caagtccgta tccattgcga ctttcccagt
     5881 gtccgcagct tcctgctcca tgttttgagt tcatttgccg aacacgcgcc tgccgatacc
     5941 aacatcatca tcaagcatca tccgatggac cgcggtttta tcgactactg gcgcgacatt
     6001 aaacgcttta tcaaagaaca ccccgaactc aaaggccgcg tgatttatgt tcatgatgtc
     6061 cccctgcccg ttttcctgcg ccacggtctc ggcatggtca ccatcaacag caccagcggc
     6121 ctgtccggcc tgattcacaa tatgccagtt aaggttctcg gccgtgccta ttatgatatt
     6181 cccggcatta ctgaccaaaa taccttggca gaattttgga atcatccgac accgcctgac
     6241 aaagagctgt ttcatgccta ccgtatgtac cacctcaacg tgacccaaat taacggcaac
     6301 ttctacagtc aggtgttttt ccccaacaaa aaaacctcca actcttccac accagtaatc
     6361 tgacttagcg aaggaaagtt caggccgtct gaaaacattt cagacggcct gaaacaatca
     6421 ataccttagc tactgccatg taaataaaac acaaaaacct gcatttatca ttaacaataa
     6481 attacaaaaa cagtataatg accgagctgt catgagcgca taccgactca acctgagccc
     6541 tttgtaacac acaaaatatg gatgtatccc taggcaaaac aatataacaa gccaaacatc
     6601 ctaaagataa gccggcaagg caacacactc tacaaaaccg tgccaagcaa tatttttaca
     6661 aagccctcaa ccggtatcgc cgcccatatg ccgcagcatc cgtcttccac tttatatccg
     6721 cccgcaaacc atgaccgccg ctcctgatat cctctaccgg caagccgccg cccttttgga
     6781 acaatccaat accgcccaag ccctgcccct gctgcaacag gccgcagagc agggatatac
     6841 ggaagctgct tttgtattgg gcaaccatct gctgaaaaac ggtcatccgg agcaggcact
     6901 tccatggttg gaagccgccg cggcccaacg ccatcccaag gcactcttca ccctgctgca
     6961 acaacgcgaa cacaacggca ccccgaccgg acagcttctt aacgaatacg cctggctgag
     7021 tgagcaaggt cacctcgaag cccaattaat tctcatgcgt taccacacgc agcgcaacga
     7081 tccacaatcg ctctactggg cggagcttgc cgctgcccaa tatgccgcac ctgcgtatta
     7141 ccatttggca cgccatcatc aacgccaagg cgacgttgcg acagccatcg aacaatacga
     7201 aaaagcggca gcactcggca taactgctgc ctgctggcaa ctcggtcaaa tctacttcta
     7261 cggtacaggt gtcagcccca accacgcaca agccgaacac tatctcgaac aagcggcgca
     7321 agccggccac atcgccgcac aaaccctgct ggccgacctt cttgccgccc aacgcaaacc
     7381 cgaagcctta gaatggtatc gtcgtgccgc caataaggag caagcggaag cacaagccaa
     7441 gctggcccaa tacgccctga ccggcgaact ttccgaacgc gatccgttcc aagcggcgcg
     7501 ctatgccaaa gccgctgccg agaaagacca tcctgaagcc ctgaaaatca tgggcgacct
     7561 ctaccgctac ggcctcggca tcaaagctga caaccatatc gcgcacgatt actaccaccg
     7621 tgctgccgcg ctgggttctg ccgccgcagc acaaaaactc atcagcgacg ccgcgctgta
     7681 ccatccgcaa caatacgaac aaatcaaaac tgccgccctg caacaacaac aaaccgaaac
     7741 catctaccgt ttggcggaag cacaagcctg cgccatcggc cgtcccgccg actacaatgc
     7801 cgcgcgaaaa aattacatgg aagctgccgg gttccaccat aaaaacgcag cggcagcctt
     7861 aggccgcatc taccattacg gcctcggtac ggcgcaagat cctcgggcgg ctgcacactg
     7921 gtacgccatt gctgccgaac aaaaccaccc ttccgcccaa taccacctcg cctgttttta
     7981 ctatcacggg caaggtgtcg gctgtcatgt tccgaccgcc tgctactggc tgcaggccgc
     8041 catcggcaac ggccacactt cggccgaatc attaatatcc ctattagaac aatggcgacg
     8101 cgaagcacac catgccatcg gacaaaaggc cgtctgaaaa gatttacact cgcatttttt
     8161 gacaatcttt aactattccc ctaatatttg ccagttattt ttcacggaca cgccattgtt
     8221 ttcatttctt tctgaaaaca ccttgtccgc gcatcaatac catgacactc ggcggataac
     8281 gccaagcgtt gaaacacact acatccggaa caaaaacgga tgctcggaaa aatatttcta
     8341 ggaggtgaaa caacatggaa tgggaattca acagttatta cacactgatt gccgccacgc
     8401 tcgtgttgct ggttggtaaa tttctggttc aaaaaatcaa attcttacga gacttcaata
     8461 ttcccgagcc ggtagccggc ggtttgattg ccgctatcgt cctgttcgcc ctgcacgagg
     8521 cgtacggcgt gagcttcaaa tttgagaaac cgctgcaaaa tgcgtttatg ctgattttct
     8581 tcacgtccat cggcttgagc gcggattttt cccgtttgaa ggcgggcggt ttgccgctgg
     8641 tggtttttac cgcgattgtg ggcggattta tcttggtgca aaactttgtc ggggtcggac
     8701 tggctacggc tttgggtttg gatccgctca tcggtctgat taccggttcg gtgtcgctga
     8761 cgggcggaca cggtacgtca ggtgcgtggg gacctaattt tgaaacgcaa tacggcttgg
     8821 tcggcgcaac cggtttgggt attgcatcgg ctactttcgg gctggtgttc ggcggcctga
     8881 tcggcgggcc ggttgcgcgc cgcctgatca acaaaatggg ccgcaaaccg gttgaaaaca
     8941 aaaaacagga tcaggacgac aacgcggacg acgtgttcga gcaggcaaaa cgcacccgcc
     9001 tgattacggc ggaatctgcc gttgaaacgc ttgccatgtt tgccgcgtgt ttggcgtttg
     9061 ccgagattat ggacggcttc gacaaagaat atctgttcga cctgcccaaa ttcgtgtggt
     9121 gtctgtttgg cggcgtggtc atccgcaaca tcctcactgc cgcattcaag gtcaatatgt
     9181 tcgaccgcgc catcgatgtg ttcggcaatg cttcgctttc gcttttcttg gcaatggcgt
     9241 tgctgaattt gaaactgtgg gagctgaccg gtttggcggg gcctgtaacc gtgattcttg
     9301 ccgtacaaac cgtggtgatg gttttgtacg cgacttttgt tacctatgtc tttatggggc
     9361 gcgactatga tgcggcagta ttggctgccg gccattgcgg tttcggcttg ggtgcaacgc
     9421 cgacggcggt ggcaaatatg cagtccgtca cgcatacttt cggcgcgtcg cataaggcgt
     9481 ttttgattgt gcctatggtc ggcgcgttct tcgtcgattt gattaatgcc gcgattctca
     9541 ccggttttgt gaatttcttt aaaggctgat tttccgcctt tccgacaaag cacctgcaag
     9601 gtttaccgcc tgcaggtgct tttgctatga tagccgctat cggtctgcac cgtttggaag
     9661 gaacatcatg tatcggaaac tcattgcgct gccgtttgcc ctgctgcttg ccgcttgcgg
     9721 cagggaagaa ccgcccaagg cattggaatg cgccaacccc gccgtgttgc aaggcatacg
     9781 cggcaatatt caggaaacgc tcacgcagga agcgcgttct ttcgcgcgcg aagacggcag
     9841 gcagtttgtc gatgccgaca aaattatcgc cgccgcctac ggtttggcgt tttctttgga
     9901 acacgcttcg gaaacgcagg aaggcgggcg cacgttctgt atcgccgatt tgaacattac
     9961 cgtgccgtct gaaacgcttg ccgatgccaa ggcaaacagc cccctgctgt acggggaaac
    10021 cgctttgtcg gatattgtgc ggcagaagac gggcggcaat gtcgagttta aagacggcgt
    10081 attgacggca gccgtccgct tcctgcccgt caaagacggt cagacggcat ttgtcgacaa
    10141 cacggtcggt atggcggcgc aaacgctgtc tgtcgcgttg ctgccttacg gcgtgaagag
    10201 catcgtgatg atagacggca aggcggtaaa aaaagaagac gcggtcagga ttttgagcgg
    10261 aaaagcccgt gaagaagaac cgtccaaacc cacgcccgaa gacattttgg aacataatgc
    10321 cgccggaggg gatgcagacg taccccaagc cgcagaaggc gcgcccgaac cggaaatcct
    10381 gcatcctgac gacggcgagc gtgccgatac cgttaccgta tcacggggcg aagtggaaga
    10441 ggcgcgcgta caaaaccagc gtgcggaatc cgaaattacc aaactttggg gaggactcga
    10501 taccgacgtg caaaaagagt tggtcggcga acaacgcaag tgggcgcagg aaaaaatcag
    10561 caactgccga caagccgccg cgcaggcaga ccggcaggaa tacgccgaat acctcaagct
    10621 gcaatgcgac acgcggatga cgcgcgaacg gatacagtat cttcgcggct attccatcga
    10681 ttaggggcaa accgatgaat accgtcccaa aaagcaggat tcccgtcaaa ccgctgcccg
    10741 aaaaaaccac agacgaagcc aaagtcgaaa aatggcggca gctcggtgcg gaacacggtt
    10801 tgtcgggcga atgggcagtt gccgtcagat tgggcgaaaa cggttttacc gaagaacaga
    10861 tggaaaatat cgccaacctg ttcggcagat aaagagaaaa ttgacggaaa tgccgtctga
    10921 aaccctgtta tcggtttcag acggcatttt gaccaatacg gtacgcaggc gcaaaacagc
    10981 cggcttttcc tgtgttgcct atgctgatgt ttcaacacac aggacgatac aaaaaacgtc
    11041 gccctatgtg ccgtcctgat tcggaagggt tacgctcctt ccaaatatag tggattaaca
    11101 aaaaccggta c
//
LOCUS       DAWKOG010000053        10949 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.053, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000053 DAWKOG010000000
VERSION     DAWKOG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10949
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.053"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..546)
                     /locus_tag="WJK66_001039"
     CDS             complement(<1..546)
                     /locus_tag="WJK66_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7578763.1"
                     /translation="MFCTVSHLRRLKGHDVLLTAFARALAQCPQLRLNIGGSGQEEQR
                     LKQQAADLGITHAVTFLGALQPEAVLDLMRNSDAFVLASRTETFGVVYIEALSQGLPV
                     IATRCGGAESIVSDGNGYLVPVDDDDALADALIKMYEHHSDFEPARLRENCLNEFGEN
                     AVIGRLIGIFRQAIAEYGKKIP"
     gene            complement(578..961)
                     /locus_tag="WJK66_001040"
     CDS             complement(578..961)
                     /locus_tag="WJK66_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7578764.1"
                     /translation="MYFFPRFPFIDIDCIRWVCAGLKAFKHYIRENGLPDLIHAHRMN
                     YAGILAQKISQKYGIPYVVTEHSSTITRGLIRPHQWQPMEKAAAHASALLAVSRHFAH
                     VLQHKYGCEWQYLPQHTGRNIQPTV"
     gene            1330..2034
                     /locus_tag="WJK66_001041"
     CDS             1330..2034
                     /locus_tag="WJK66_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEZ7578765.1"
                     /translation="MLRSILAASLLAVSFPTAAEALNYNIVEFSESAGVEAVQDTMSA
                     RFQVTAEGRDKNAVNAEFVKKFNNFTRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDALNRFIADVQADAALEYTDFHVSRERRNEVIDQVSKDAVLRFK
                     ARAEKLAGVLGASGYKIVKLNLGHIGSHIAGGGAAQAKMLRAMPMAASVNMEGADSAA
                     PGVEEISISVNGTVQF"
     gene            complement(2098..2664)
                     /locus_tag="WJK66_001042"
     CDS             complement(2098..2664)
                     /locus_tag="WJK66_001042"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEZ7578766.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(2730..3146)
                     /locus_tag="WJK66_001043"
     CDS             complement(2730..3146)
                     /locus_tag="WJK66_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578767.1"
                     /translation="MLKHLAFLLPAMMFALPAASAVLTSYQEPGCTYEGDVGKDGKPA
                     GKGTWRCQDGRNYTGSFKNGKFDGQGVYTVAANREIFIEPFNSDSTKFRNMVLSGTFK
                     KGLAHGRFTVSQNGETLFIMKCENGMIKEVKLPKNK"
     gene            complement(3198..4097)
                     /locus_tag="WJK66_001044"
     CDS             complement(3198..4097)
                     /locus_tag="WJK66_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEZ7578768.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSIMLEELVSYLGGWQD"
     gene            complement(4128..5585)
                     /gene="der"
                     /locus_tag="WJK66_001045"
     CDS             complement(4128..5585)
                     /gene="der"
                     /locus_tag="WJK66_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEZ7578769.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEDIL
                     ETFPEPEKEEEEAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENADDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            complement(5739..6368)
                     /locus_tag="WJK66_001046"
     CDS             complement(5739..6368)
                     /locus_tag="WJK66_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HEZ7578770.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFAVLILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQSEINAELAKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            complement(6369..7664)
                     /gene="hisS"
                     /locus_tag="WJK66_001047"
     CDS             complement(6369..7664)
                     /gene="hisS"
                     /locus_tag="WJK66_001047"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEZ7578771.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVGRWTRTYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNFLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGEGADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAADLTNTLQQWKNA"
     gene            complement(7758..8300)
                     /locus_tag="WJK66_001048"
     CDS             complement(7758..8300)
                     /locus_tag="WJK66_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ7578772.1"
                     /translation="MQSWKERRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVL
                     KKLDKEQMRASFEDMRRIMPDLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVL
                     RGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKKAETISNKLFFT
                     HHPKRQTEFAVGQIRQARAE"
     gene            complement(8696..9232)
                     /locus_tag="WJK66_001049"
     CDS             complement(8696..9232)
                     /locus_tag="WJK66_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578773.1"
                     /translation="MKKIIDSKVRETNFNQAWRVIRAMQIDNISDEQVVIVRMSCYLK
                     KDEIFKNIERYGYQSSDIKKISPPFPLNILPVPKPYYFYLYDTHLNQIYDLAWSCNDF
                     LMFEIFILDRESIKYIDKDKLFKSGETLYSNFKSIKNQCKNYFFCGFDFDAPYFKSGV
                     SQVADYNFVPDAFSLFFI"
     gene            complement(9400..9600)
                     /locus_tag="WJK66_001050"
     CDS             complement(9400..9600)
                     /locus_tag="WJK66_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578774.1"
                     /translation="MQLICADWTGIGNFMKTFEKTWSAQYRDMEISVRNFWNLERTGA
                     EVYINGRRVYHNEAEMASASLR"
     gene            complement(9715..10134)
                     /locus_tag="WJK66_001051"
     CDS             complement(9715..10134)
                     /locus_tag="WJK66_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578775.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGEWLKHIYEFNRGSFIFSYVLLKKRESEENFAEIVLKSIKNN
                     KILTVKVRSGLHFDLRNIYRIEMYSGLNLNQYGVASP"
     gene            complement(10192..10557)
                     /locus_tag="WJK66_001052"
     CDS             complement(10192..10557)
                     /locus_tag="WJK66_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578776.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(10539..>10949)
                     /locus_tag="WJK66_001053"
     CDS             complement(10539..>10949)
                     /locus_tag="WJK66_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578777.1"
                     /translation="LSQKEMKETERAFLPLAILGGAAIGMWTQHGFSYATTGRPASVR
                     DVAIAGGLGAIPGGVGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHR
                     RVTDNTGKTLPGQGIGRHRPWESKSTDRSWKNRF"
BASE COUNT         2680 a         2624 c         2642 g         3003 t
ORIGIN      
        1 cggtattttc ttaccgtatt ctgcgattgc ctgtcggaaa atgccgatca acctgcctat
       61 aacggcattt tcgccaaatt cgttcagaca gttttcccta agtcgggcag gttcaaaatc
      121 agagtggtgt tcatacattt tgatgagtgc gtcggcaagg gcatcgtcgt cgtcaacagg
      181 aaccaaatat ccgttgccgt ctgaaacaat agattccgca ccgccgcagc gtgtcgcaat
      241 gacgggcaat ccttgggaca gtgcttcgat atagaccacg ccgaaggttt cggtgcggct
      301 ggcaaggacg aatgcgtcgc tgttcctcat caaatccaag actgcttcgg gctgcaatgc
      361 gcccaaaaat gtaacggcat gggtaatgcc caagtctgcc gcctgctgtt tcagccgctg
      421 ttcttcctgt ccgctgccgc cgatgttcag gcgcagttgc gggcattgtg ccaacgcccg
      481 ggcaaaggca gtgagtagga catcgtgtcc tttgagacgg cgaaggtgcg agacggtgca
      541 gaacacgaaa tgcggtttgt tgtttttttc tctttgttca aacggtcggt tgaatattcc
      601 gcccagtatg ttgggggagg tactgccatt cgcagccgta tttgtgttgc aggacgtgtg
      661 cgaaatggcg gctgacagcg agcagtgcgg aggcgtgcgc cgctgctttt tccatcggct
      721 gccattggtg gggacggatc agaccgcgcg tgatggtgct gctgtgttcc gtgacgacat
      781 aggggatgcc gtatttttgg gaaatttttt gggcgagtat gccggcatag ttcatgcggt
      841 gggcgtgaat caggtcgggc agcccgtttt cgcggatgta gtgtttgaaa gctttcaaac
      901 ccgcacacac ccagcggatg cagtcgatgt cgatgaacgg aaagcggggg aagaaatata
      961 tgccgtgcca tgcatagatg tccaaaccgc tttgccgata ttttgcaaaa ccgtaaggcc
     1021 ggtcaggatg cttgctgttt ctttccgcaa gtagcggaac atcggtgcaa gcacggcggt
     1081 tttgatgcct ttcctctgca atgccagtgc ctgattttga aaaaaatccc gtccacatcc
     1141 tgttcggatt gcggatacca tgaggggatg acgaggacgt gcaagggttc gggcatagtg
     1201 ggattccgta tcggaaaggc ggttattata agacagacgc agaccgaata tttaaattgt
     1261 tgccttacgc taatgcaatt tagcgcgccg atatgctaga ttggcggttt tataagtaag
     1321 gatacggata tgttgcgttc tattttggcg gcttccctgc tggcggtatc ttttccgacg
     1381 gcggctgaag cattgaatta caatattgtc gaattttctg aatcggcggg tgtcgaggcg
     1441 gttcaggata caatgtccgc acgtttccaa gtgacggcgg aaggacggga caaaaatgcc
     1501 gtcaatgccg agtttgttaa aaaattcaac aatttcacca gaaaatcaaa aaatggtagc
     1561 tttaaaaccg aattggtatc gcgcagtgcg atgccgcgct atcaatatac caacggcaga
     1621 cgcattcaaa caggttggga ggagcgtgcg gaatttaagg tcgagggtag ggattttgat
     1681 gcgttgaacc gttttattgc cgatgttcag gcagatgccg cgttggaata tacggatttc
     1741 catgtgtcgc gcgaacgccg caacgaggtc atcgatcagg tcagcaagga tgccgttttg
     1801 cgtttcaagg cgcgtgccga aaagttggcg ggcgttttgg gtgcgtccgg ttataaaatc
     1861 gtcaaattga atttgggaca catcggcagc catatcgcgg gagggggagc tgctcaggca
     1921 aaaatgcttc gtgccatgcc gatggcggca agcgtcaata tggagggtgc ggattccgcc
     1981 gcgcctggtg tggaggaaat cagcatcagc gtcaatggga cggttcagtt ctgatttgag
     2041 gtgaacggca aatgccgtct gaaacccgac gataagggtt cagacggcat ttatatttca
     2101 ggctttgggc agggtaacgc cggtttgccc catatatttg ccgttgcggt ctttgtatga
     2161 ggtttcgcag atttcgtcgc tctcgaagaa gaggacttgc gccacgcctt cgcctgcgta
     2221 gattttggcg ggcagggggg tggtgttgga aaattcgagg gtaacgtagc cttcccattc
     2281 cggttcgaac ggggtaacgt tgacgataat gccgcagcgg gcgtaggtgg atttgcccaa
     2341 gcagacggtc aggacgttgc gcgggatgcg gaaatattcg accgtgcgcg ccagtgcgaa
     2401 ggaattgggc gggatgatgc agcagtcgtc ttcaacggta acgaagtttt tcgggtcgaa
     2461 gtttttggga tcgacgatgg tgctgttgat gttggtgaag attttaaatt cgtttgcgca
     2521 gcggatgtcg tagccgtagc tggacgtacc gtaggagatg atgcgtttgc cgtcggcttc
     2581 tttgatttgg ttcggctcga aagggtcgat catgccgaat tcttcgctca ttcggcgtat
     2641 ccatttgtcg gacttgatgc tcataatgtt ttccttgttt cttgcagtgt tcggacaaag
     2701 cattggggga tgccgtctga aaacggggct tatttgtttt tgggcagctt cacttcttta
     2761 atcatgccgt tttcgcattt cataatgaag agggtttcgc cgttttgcga gacggtaaat
     2821 ctgccgtgtg ccaagccttt tttgaatgtg cccgagagta ccatgttgcg gaatttggta
     2881 ctgtcggaat tgaacggttc gataaatatt tcgcggttgg cggcaacggt ataaacgcct
     2941 tgtccgtcga atttgccatt tttaaacgaa ccggtatagt tgcgcccgtc ttggcagcgc
     3001 catgtgcctt tgccggcggg tttaccgtct ttgccgacat cgccttcgta ggtgcagccg
     3061 ggttcttgat aggaagtcag aacggcggac gcggcgggga gggcgaacat catggcgggc
     3121 agtaggaatg cgagatgttt aagcataagg gttattccat tggattttgg ctgacggtat
     3181 aaaagccgtc tgaaaaatca atcttgccag ccgcccaaat aggaaaccag ctcttccaac
     3241 atgatgctga cggattccgc catcaggatt tgcgaggcga aggcgagacc ggcggcatcg
     3301 tcgccgttgc tttcggcttc ttcctgcaat acgtcgaggt attggatgcg cttgagtgtg
     3361 aagtcttgcg tgaggataaa ggcaatttgt tcgcgccaca ccaagcccag ttgggtaacg
     3421 gttttaccgt ttttgacgtg ttgaaccact tcgtcggcgg ttaaatcttg tttggatact
     3481 ttgacgacgg gaacaatatc gcccgtgcct ttgagttcgc aatcgctgtc caattcaaaa
     3541 ccgccttcgc aatgcccttg caacagccag ccggtcatca gggaagaggg cgattgtttg
     3601 gtattcggca gcgaggcttc caaaccgccc aaagcttcgc gcagcttggt caggatgttt
     3661 tccgctttgg cggaagccgc gttattgacg agcaggtagc cgtggcgggt gttaaacacc
     3721 gcttctgtac ggctgctgcg ggtaaacgct cggggcagca ggtcgtctgt aatttgctct
     3781 ttaagctctt gtttttcttt gcgaccgaca ttgcgggctt cattgttttg gatttccgct
     3841 accttctctt ccaaaatatc gcggatgacg ccggcaggca ggactttttc ctcttttttc
     3901 agggcgacgc gcaaggtaaa gtcggcaggg aaaacgagtt ccggggagaa tgaaaccggt
     3961 gcggtaaagc cttcgctgaa ccagtctaag ccttggcaat gggtaaattc agcttcagca
     4021 agtttgtcgg caagtacgtc cgcctcaggc agcttttctt tgttgagcgg ataaaaacta
     4081 atctgcttga accacataat gtttcctgtt gtttgaaatg tcgggaatta tttgctgaat
     4141 tgttttttca cactgacttt ggttttcttc ttgaagcggt ttttctcttc tttgcggcct
     4201 tcgcgtttct caatacggtt gctcaggctg acgcggcgca gcggtttttt cttcggtttg
     4261 tcgtcggcgt tttcatacgg gttttccgaa acattgtatt gaatccgcaa cggcgtgcct
     4321 tgcaggttga aggctttgcg gaacgtttgg gtcagatagc gcgtatagct gtcggaaatc
     4381 gcgtgcagcg aattgccgtg taccacaatt acgggagggt tcatgccgcc ttggtgggca
     4441 taacgcattt tcggacgcac caagccggca cgcggcggtt gctgacgctc gattgcgctt
     4501 tgcaggacgc gcgtgatttt cggcgtcggc atcttaatca tcgccgcgtt gtaggcagcc
     4561 tgaatgctgt caaacaaacc gtctataccg cgctctttca atgcggaaat aaagtggaac
     4621 ttggcaaaat cgaggaaata cagtttgcgg ttgatatcgc gtttcacttg ctcgcgacgt
     4681 tcttcgctga tgccgtccca tttattgacc gccaccacca aagcacggcc tgcctccaaa
     4741 gcgaaacctg caatcgtcgc atcttggtcg gcgatgtcct gctgcgcgtc caataccaaa
     4801 acagcgacgt ttgccgcttc aaccgcctgc atcgctttga taacggagaa tttttccact
     4861 gcttcatcca ctttgccgcg acggcgcaca cctgcggtat cgatgatggt aaacggtttg
     4921 ccttcgcgct cgaaatcgat atggatgctg tcgcgcgtcg tacctgccat atcgaaggcg
     4981 atgacgcgct cttcgccgag aatggcgttt accaaggttg atttaccgac atttggacga
     5041 ccgataacgg caaaaacagg atgtttcgcc tcttcctctt ctttttcagg ctcagggaag
     5101 gtttccaaaa tatcttcaat cagataatac acgccgtcac cgtgcgcacc tgaaataaca
     5161 taagggtcgc ccaaagcaag ttcgtagaac tcggcggcaa gcacggccct gttgccgcct
     5221 tctcctttat tcacggccaa ataaacgggg cgcgggcttt ggcgcaaacg gtcggcaatg
     5281 attttatctt gcggcgtcag accggtacgg ccgtccacca aaaacacaac cgcatcggct
     5341 tcatcgacag cctgtaaggt ttgttttgcc atttcgtgca aaatgccgct gtccacaacc
     5401 ggctcgaaac cgccggtatc gatgaccaaa taaggtttgc tgccgacttt gccgtgtccg
     5461 taatggcggt cgcgggtcag gccgggcagg tcgtgtacga gtgcgtcttt ggtgcgcgtc
     5521 aaacggttga ataaagtaga tttgccgacg ttggggcggc cgacaagcgc gatggttggt
     5581 ttcatgattg agtctttctg tgtcaagtgc cgttcgggag aactgaacac gagcaggtgt
     5641 ccgttggaca cggtaaatag gttcttcacg gcaagaggcc ataaagaggg agggtaaaat
     5701 gccgtctgat aaataatgga cggcatttca gacggcattt atttcagcga atcaagtttc
     5761 atttgaacca attcgcgacc gacagaatct tgaggcattt tttctaaagc ctgtccgtag
     5821 ttttttaagg cttcctggct ttttccctgt gcggcataga catcgccttt ggtttccatc
     5881 agcagggggg cgaagtccgc ttcaaccggc gtgtcgagtg cggcaagcgc ggcatcgtat
     5941 tttttttgtt gcaacaacac aacgcccaga cgctgcgccg ccaacgcctg gatcaggctg
     6001 tctttttggt tggacaatac ccatttcaaa tggccttcgg caacatcgta acgctgcgcg
     6061 tcaaattcgg ttgctgccgc catcagcgtg gcttgggcgg cggaaatgga atgggggtag
     6121 ctttgttgga gcttggccaa ttcggcattg atttcgcttt gcggggcttt gttttgcgcc
     6181 ttttccacga tgtttgccag caccgccgcc gcttcctgat tttgggaagc cgcacggttt
     6241 tggtaaaccg tgtatcccaa gtagccgagt gccgccaaaa tcagcacggc aaacagccat
     6301 ttgcccgtgg ttttccaaaa atatttaaag ttgtccaact cttgttgttc ttccaaatgg
     6361 gctgccattt atgcgttctt ccattgttgt aaagtgtttg tcaaatcagc ggcggcgacg
     6421 gtttgctgac cgtgtgcgcc gttcatatct ttgagcgtaa ccgtaccgtt cgccagttcg
     6481 tcttgcgcga caatcagggc aaagcgtgcg ccgctgttgt cggctttttt catttgcgct
     6541 ttcaggctct gatagccgga atgctgcatc acattgaaac cttgcgcgcg caaggcttgt
     6601 gcgtatttca tcacctgcaa gtccgcccct tcgccttggt gcattgcata gacatcaggc
     6661 gcggcgttta cttccagcga gccgtattcg ctcaccaaaa gcagcagccg ctcgatgccc
     6721 attgcaaagc cgatagacgg cgcaggctta ccaccaagtt cctcaatcaa accgtcgtaa
     6781 cggccgccgc cgcacacagt tgcctgcgcg ccgagtttgt cggtcgtcca ctcaaaaacc
     6841 gtttgattgt aataatccaa accgcgaacc aagcgcggat tttcaatata ttggataccc
     6901 aaaccgtcca acatcgcttt gaagcgtgca tagtggtttt gcgaagcctc gcccaaataa
     6961 tccaccagac gcggtgccgc attgcagatt tcctgcaaat cagggttttt cgtatccaaa
     7021 acgcgcaaag gattggtttt cagacggcgt ttgctgtctt catccaattt atcttcataa
     7081 cgggtcagat attcaaccaa tgccgcacgg tgtgccgcgc gttcctcacg gttgcccaag
     7141 ctgttgattt ccaaagtcag gtattcgcgg atacccaatt tttcccacaa gtcggcagac
     7201 atcgcgataa tttccgcatc gatatccggc ccttcaaaac ccaaagcctc gataccgacc
     7261 tgatggaact gacgataacg gcctttttgc ggacgctcgc ggcggaacat cggccccata
     7321 taccacagct tttgcgggct gttgtacaga aagttgtgtt cgaccaccgc acgcaggcag
     7381 gaagccgtac cttcaggacg caagctcaaa ctcaaagaat cgtttgaatc ggagaaggtg
     7441 tacatttcct tgccgaccac atcggtttcc tcgccgatgg agcggacaaa caaaccggtt
     7501 tgctcgacaa tcggcgtacg gatttgctga taaccgtaag tgcgtgtcca gcggccgacc
     7561 gtatcttcaa acgcctgcca aaacgcagcc gtcagtttga aatctttttg ctcgacaggc
     7621 agaaggtcgt tcatgccttt gacggattgg attttttgtg ccatttcaag taagaatgct
     7681 taaatcaaat tgcgggcgat tatagcggat ttttaagggt ttgtgaggtt gggggtggtt
     7741 tgcggacggc atttgactta ctctgcacgt gcttgcctga tttgtccgac tgcaaactcc
     7801 gtctgccgct tgggatgatg tgtgaaaaac aatttatttg aaattgtctc ggcttttttc
     7861 ggcacgaccg ccaaaatctt acctgccaaa tttccctcac gggtttgcca agcatccaaa
     7921 aactgcgccc tgctcattga aacatgaccc agcgacgggt cggcaagcaa aaccgtattg
     7981 ccgtctatcc cgcgcaatac cgagaaatga tcatccttgc ggtatttcag atacacgatg
     8041 acggggattt tcaactgtgc gagctgctcg aaagacaggg catagccttt cgcttcaaaa
     8101 cccaaatctg gcataatgcg ccgcatatcc tcaaacgacg cgcgcatctg ctccttatcc
     8161 agctttttca acacttcttc ttccgtcagc gtttgcccgt aaaaattgtt caaaagcgtc
     8221 gccaccgaag ccgccccgca ggaaaaatcc aaatcctgct ttacaatatt gaaatcccgc
     8281 ctttctttcc aactctgcac tttgattttt ccgtaaacaa caggattatc gttaaacatc
     8341 ggtgcagcat tcaaacgata agataaagaa acgacaacac acgccaacag aaaaacatat
     8401 ttgaacttca tcatattgtc cacataaagg gcagcctgaa aatctttcag gctgcccttg
     8461 tcaaattatt cctagctttc ggcttttttg gcaaaccaaa caatccgatt acccgcataa
     8521 tactttccat ttattgaaat ccgacaagcc gcgcccaaaa aatgccatgc actgtcgatt
     8581 tccgcagtaa tctttgtatc gttttcttca aattccaaat attcacccaa taataaactt
     8641 gaaacagaac gcgtgggcag caagtgccca ccctacgcct attcaaataa tttgattaaa
     8701 taaagaataa agaaaatgca tcaggaacaa aattataatc tgccacctga ctcacaccgc
     8761 ttttaaagta aggggcatca aaatcaaaac cgcaaaaaaa ataatttttg cattgatttt
     8821 taatagattt aaaattcgaa tatagtgttt ctccagattt aaaaagttta tctttatcta
     8881 tatatttgat gctttcccta tccaaaataa atatttcaaa cattaaaaaa tcattacatg
     8941 accaagccaa atcataaatt tgattcaaat gggtatcata aagataaaaa taataaggtt
     9001 tgggaacagg taaaatattt aatggaaaag gaggactaat tttcttaata tctgatgact
     9061 gatatccata tctttctata ttcttaaaaa tttcatcttt cttaagataa caagacattc
     9121 tgacaatcac aacctgttca tcggatatat tatctatttg catggcgcgt ataacacgcc
     9181 atgcctgatt aaaattagtc tcccttacct tagaatctat tatctttttc aaagatgaaa
     9241 atgcccctta tcttgaactc tccagttaga acctggaagt ttcgtacctc ttaaatgtgt
     9301 attaatattt cttatagttt cattaaaatg ccacgcgctg ccgatttcaa cggtaatttt
     9361 cgtaccgctt tcttcaaatt ccaggtattc ccccattagc taacgcaaag aagcagacgc
     9421 catttcggct tcgttatgat aaacccgcct tccgttgata tagacttccg cccctgtccg
     9481 ctccaaattc caaaaattcc gtactgaaat ttccatatcc cgatattgtg cagaccatgt
     9541 tttttcgaag gttttcataa aatttcctat acctgtccaa tcggcacata tcaattgcat
     9601 tattatagtg gattaacaaa aatcaggaca aggcaacgaa gccgcagaca gtacaaatag
     9661 tacagaaccg attcacttgg tgtttcagca ccttagagaa tcgttctctt tgagctaagg
     9721 tgaggcaacg ccgtactggt ttaaatttaa tccgctatac atctcaatac gataaatatt
     9781 ccttaagtca aaatgcaagc ctgaccgtac cttaactgtc aaaattttat tattttttat
     9841 tgattttaaa acaatttctg caaaattctc ttcgctttct ctctttttta gaagcacata
     9901 agaaaaaata aaacttcccc gattaaattc ataaatatgt ttcaaccatt cgcctcctct
     9961 ttctgtaaga caagattcag tttcattctt ccttattgta taaatatttc cttcacaaaa
    10021 tctgaaataa atccataaat ccatcttatc cataattaaa gaaaaagttt cacctcgaga
    10081 ttttgtcaac aattcgcaag gttgcgatgt tgcaatcaaa tagccgaaag acatttttta
    10141 cctcatacat ggtcgaaatc agtttctgtt agttcagaat ccattttttc gtcaacaact
    10201 gaatccgcat ttttgaatta acgttttcat cagctgccgt ttatctaaac cggcaggttc
    10261 agtttcaaaa taagccttat atgaagactg taagcatttc agaaaaagat catcagaaga
    10321 catatctgcc gaatcaaata caactgtttt gattttggta cttacccaaa accctttttg
    10381 ctctttttct actatacgga aattcagaat atttccaacc gaatcaaaag cacggtaaac
    10441 atcatccatc aaatcctgcg gctctatttt cttttccaat tccgacaatc cttgaaatat
    10501 atccaaagac acatcttcaa atagaaaaaa aggaggagtt agaagcggtt tttccatgat
    10561 ctgtccgtag attttgattc ccaagggcga tgacgaccaa ttccctgtcc aggcaaagtc
    10621 ttgcccgtat tatccgtaac tcgacgatga taatggggaa attttccaat aggatgacct
    10681 gttctattac cgaaaggggc tatccgcata ttattgccga ttttaatctc acgtccatat
    10741 ttagcaaagg aaacaacctt tcctgcggcg cctacaccac caggaattgc gcctaatccg
    10801 ccagcaatag caacatctct aacagaagct ggtctgcctg tcgttgcata actaaaacca
    10861 tgctgtgtcc acataccaat ggcagcacca cccaagatag ccaatggaag aaacgccctc
    10921 tcagtctcct tcatctcctt ttgagaaag
//
LOCUS       DAWKOG010000054        10925 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.054, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000054 DAWKOG010000000
VERSION     DAWKOG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10925
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.054"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(81..1226)
                     /gene="dapE"
                     /locus_tag="WJK66_001054"
     CDS             complement(81..1226)
                     /gene="dapE"
                     /locus_tag="WJK66_001054"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEZ7578778.1"
                     /translation="MTETQSLELAKELISRPSVTPDDQNCQQLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKVPVVCFAGHTDVVPTGPVEKWDSPPFEPTERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARGELIDYC
                     IVGEPTAVGKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTHAGKLTDVARAAIAEICGVETELSTTGGTSDGRFIKA
                     IAKELIELGSSNATIHQINENVRLDDIPKLSAVYEGILARLLAGNAV"
     gene            complement(1340..1960)
                     /locus_tag="WJK66_001055"
     CDS             complement(1340..1960)
                     /locus_tag="WJK66_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEZ7578779.1"
                     /translation="MLTPKSCDLFNIPFFQFSQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPDYNRWLDNFDTEKYASFDMWHGAESEYDDYRTVAQQNESDR
                     KLQNDEDFFCIGKHIERDDLGRQDVAKWIAETVEDLLPLYEACHGK"
     gene            complement(1994..2503)
                     /locus_tag="WJK66_001056"
     CDS             complement(1994..2503)
                     /locus_tag="WJK66_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ7578780.1"
                     /translation="MNPFETKSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQIVLQTIRQIARYFNVAAPLHHEDEEENFFPLLLQYAPQAQESVDELLRQHVSLYGN
                     WDAVAAEFAKLEADNAYVPDEEAFKRFVAGYDVHLAIEEPLFDMGNTFIPKEKLTEIG
                     EIMAARRRK"
     gene            complement(2597..3148)
                     /gene="azu"
                     /locus_tag="WJK66_001057"
     CDS             complement(2597..3148)
                     /gene="azu"
                     /locus_tag="WJK66_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HEZ7578781.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAAEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKTSMGH
                     NIVIGKTEDMDGIFKDGVGAADTDYVKPDDARVVAHTKLIGGGEESSLTLDPAKLADG
                     DYKFACTFPGHGALMNGKITLVD"
     gene            complement(3429..3851)
                     /locus_tag="WJK66_001058"
     CDS             complement(3429..3851)
                     /locus_tag="WJK66_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEZ7578782.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLAPKPEKPPKTNT
                     LHLSKAALESFGSAAVKLEKRHPELAEALANLVRRHGA"
     gene            3982..6732
                     /gene="secA"
                     /locus_tag="WJK66_001059"
     CDS             3982..6732
                     /gene="secA"
                     /locus_tag="WJK66_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEZ7578783.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTNQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPIQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSHLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADEALSDEEKQAQISALEDGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMQEIR
                     SDVVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            6876..8648
                     /locus_tag="WJK66_001060"
     CDS             6876..8648
                     /locus_tag="WJK66_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEZ7578784.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMIVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPTAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEVGRILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMLTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAESIKNHAAVPETAQV
                     LEYMRGSPYEETITRIFHSTHQSEEMNSSSEEDCENFQIGMKKLLNELKYSQIEALKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            8835..10763
                     /gene="rpoD"
                     /locus_tag="WJK66_001061"
     CDS             8835..10763
                     /gene="rpoD"
                     /locus_tag="WJK66_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEZ7578785.1"
                     /translation="MSRNQNHEEYQDDTRPLSIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
BASE COUNT         2870 a         3038 c         2684 g         2333 t
ORIGIN      
        1 aatttaatcc actatatctc aaacccacat taggtctaat caaatggtcg gacatccata
       61 tccgacagcc cattttcttt tcagacggca tttccagcca acaagcgcgc caatatcccc
      121 tcatacaccg ccgacagctt cggaatatcg tccagccgca cgttttcgtt gatttggtgg
      181 atggtcgcat tggacgagcc taattcgatg agttcttttg caatggcttt gatgaagcgt
      241 ccgtccgaag tgccgccggt ggtggacaat tcggtctcta cgccgcagat ttcggcaatg
      301 gcggcgcgtg ccacgtcggt cagtttgccc gcgtgggtca ggaagggctg ccccgaacac
      361 gaccactgca aatcgtattg cacaccgtgt ttgtccaaaa tggcgtggac gcgttgtttc
      421 agccctgctt cggtggactc ggtggagaaa cggaaattga atttgacatt cagctcgccc
      481 ggaatgacgt tggtcgcgcc tgtgccgccg ttgatattgg aaatctggaa actggtcggc
      541 gggaagtatt cgttgccttc gtcccagact tcctgcgtca gctctaacaa ggccggggca
      601 aaagtatgga cgggattgat tgccaaatgc ggataggcaa tatgaccttg cttgcctttg
      661 acggtcaggt tgcccgacag cgagccacgc cggccgtttt taatcatatc gcccaatttg
      721 cccacggcgg tcggctcgcc gacgatgcag tagtcaatca actcgccgcg cgctttcaac
      781 acatcgacga ctttggtcgt accgtccagc gcgtcgccct cttcgtcgga agtaatcagg
      841 agcgcgatgc tgccttggtg gtcgggatgt tcggcaacaa agcgttcgca ggcggtaaca
      901 aaacaggcga tgctggtttt catatccgcc gcaccgcgcc cgtataatct tccgtcgcgc
      961 tcggtcggct cgaacggggg cgaatcccat ttttcaacag ggcctgtcgg cacaacgtcg
     1021 gtatgccctg caaaacagac gacgggaact ttcgtgccgc gtcgcaacca gatgtttttg
     1081 gtgtcgccga aatggagttc ttcagccgca aaaccgattt tgtgcaggcg ttcggccagc
     1141 agttgttggc agttttggtc atctggcgtc acggatggtc gggaaatcag ctctttggca
     1201 agctctaggg attgagtttc ggtcatattt gttcactttt gaaattagac cgtctgaaac
     1261 gttctgaatg tgattttcag acggcattta ggttaggttg gcatacgggg tgggtatttt
     1321 tacccgtcag tcttctgaat catttgccgt ggcaggcttc gtaaagcggc agcaaatctt
     1381 ccaccgtttc cgctatccat ttcgcgacat cctgcctgcc caaatcgtcg cgttcgatgt
     1441 gtttgccgat gcagaaaaag tcttcgtcgt tttgcaactt tcggtcggac tcgttttgtt
     1501 gcgcgacggt gcggtagtcg tcgtattcgc tttccgcacc gtgccacata tcgaaagaag
     1561 cgtatttttc ggtatcaaaa ttatccaacc agcggttgta atcaggcagc gcgatgggcg
     1621 acacgtcggc tttgtagcag tgccaatcca agctgacgct caaacggcgg cggttgagca
     1681 gtatcgacaa aatcgcggcg gaatttttat attgttcgta tttgaagtag gcaaagaaat
     1741 gggcgcgaac ctgccagccg ttacaccagc gttcgatgtg cggaggcgca aacggcgcac
     1801 ccaattcggc ggcaacctgc tgaatcagct gctgccatat ctgccagttt tctttatagt
     1861 cagccttgat ttgcggaatg ctttcaggct ggtatttttt aagctgggaa aattggaaaa
     1921 acgggatatt gaacaaatcg caacttttcg gggtcagcat aatatatcct tgagacgatt
     1981 gtttcagacg gcattatttg cgccggcgcg ccgccataat ttcgccgatt tcggtcagtt
     2041 tttcttttgg gataaaggtg ttgcccatat caaacagcgg ctcttcaatc gccagatgca
     2101 catcgtaacc tgccacaaaa cgtttgaacg cttcctcatc ggggacataa gcgttgtctg
     2161 cttcgagttt ggcaaattcg gcagccacgg catcccagtt gccatacagg ctcacatgct
     2221 gacgcaaaag ctcgtccacg ctttcttggg cttgcggcgc gtattgcagc agcagcggaa
     2281 agaagttttc ttcttcgtct tcatggtgca gcggcgcggc aacgttgaaa taccgggcga
     2341 tttggcggat ggtttgcaaa acaatctgat tgcagccgtt ttcggcgata tagtccgaca
     2401 gcatggcgac ttgtccgcaa aaacggcgta ctttgccatg gcaggcatac agcatttcaa
     2461 tcggttcggc aaaggtaacg cttttggttt caaacggatt catggttttt tagattttat
     2521 tcttaaatta acgtttccag attataacaa aacagcctgc tcaaggcagg ctgtctgttt
     2581 tcaagcttta ggcgaattag tcaaccaaag taattttacc gttcatcaaa gcaccgtgac
     2641 ccgggaaggt gcaggcaaat ttgtagtcgc cgtcagccaa tttggcagga tctagagtca
     2701 gggaagactc ttcgccgccg ccgatcagtt tggtgtgggc aacaacgcgc gcatcgtcag
     2761 gtttaacgta gtcagtgtca gctgcgccaa cgccatcttt gaaaataccg tccatgtctt
     2821 cagttttacc gatgacaatg ttgtgaccca tgctggtttt aggttgggta ccggtgtgtt
     2881 tcagggtgat ggtgaattcc ttacaagctt tgcttacttg gatgtcttta gtgttgaact
     2941 gcatattgtc gttggattcg acagttgccg cacagttgcc ggcagcaggg gcttcggcag
     3001 catctgcagg agcagcttcg gcggcaggcg cttcggaagc gggtgcttca gcagcaggag
     3061 ttgcctcggc agcaggcgcg gcaggttctt gagagcaggc agccaaaccg ataacggcgg
     3121 cagaaatcag agccagatac gctttcataa caaatctcca atcgataaaa taatattcgg
     3181 ttttacagaa atcaaagtgc aaccgccatt aacaaaacct tgaaaaagat tccgccgcgt
     3241 tgcacaaaca gatgtttcgg agcggcattt tgctacaaat ttcatttgaa atcaaagcct
     3301 gtctgcaagt ttacaatcgt ttactttaaa aggggcaatt ttaccccgaa cctatttctt
     3361 tagtattaga cccattatcc tttacttctt aatattaacg gatgtttaca caaattcccg
     3421 tatacatttt atgcgccatg ccttctaacc aagtttgcca atgcctccgc caattcggga
     3481 tgccgttttt ccaactttac cgccgccgaa ccgaaactct ccagcgcagc cttactcaaa
     3541 tgcagggtat tggttttcgg cggtttttcc ggtttcgggg ccagcctgac cgaaacagag
     3601 cgtatcgaag catcaagccc tgccaactgc ggcaataccg acggtgcaat cattttcaag
     3661 cgcgatgccg ccatattgtt tgccgccaaa aggacaagcc tgccgtcttc gatacatgcc
     3721 gtctgaaaat gcgggtgcag gttggcaggc agcagttttt tcacggcggc atccaaccgc
     3781 cgccactgtc ccgcctgttt caaaagtccg gaaagcagcg cgtcccgcct gcccaactgt
     3841 tccaaattca taaaacatac acccaaaaag attgaaatac cgcaaacgcg cctttatttc
     3901 agacggcatt agcactttgc acaaacgctt gtgttaaaat cgcgttttcg cccactatta
     3961 tatcaggcgc aggaattatt catgctgaca aacattgcca agaaaatctt cggcagccgc
     4021 aacgaccgct tgctgaaaca ataccgtaaa tccgttgcca gaatcaacgc gctcgaagaa
     4081 cagatgcaag ccctaagcga tgctgatctg caagccaaaa ctgccgaatt caaacaacgc
     4141 ctcgccgacg gtcagacttt ggacggcatt ttgcccgaag ccttcgccgt ctgccgcgaa
     4201 gcgtcccgcc gcaccctcgg tatgcgccac ttcgacgtgc agcttatcgg cggtatggtg
     4261 ctgcacgacg gcaaaatcgc cgaaatgcgt accggcgaag gcaaaaccct ggtcgccacc
     4321 ctcgccgtct atctcaacgc gctggccggc aaaggcgtgc acgtcgttac cgtcaacgac
     4381 tacctcgcct cgcgcgacgc aggcattatg gagccgctct acaatttcct cggcctgacc
     4441 gtcggcgtga ttatttcaga tatgcaaccg ttcgaccgtc aaaacgccta tgccgccgat
     4501 atcacctacg gcaccaataa tgaattcggc ttcgactacc tgcgcgacaa tatggttacc
     4561 aaccaatacg acaaagtgca gcgcgaattg aattttgccg ttgtcgatga agtggattcc
     4621 atcttgattg acgaagcgcg cactccgctg attatctccg gccaggcaga cgacaacatc
     4681 cagttgtacc aaatcatgaa caccgtcccg ccccacctcg tccgccaaga aaccgaagaa
     4741 ggcgaaggcg actactgggt agatgaaaag gcacatcagg tcatcctgag cgaagcaggt
     4801 cacgaacacg ccgagcaaat cctgacccaa atgggattgc tggcagaaaa cgactcgctc
     4861 tattccgccg ccaatatcgc cctgatgcac caccttatgg cggcattgcg cgcgcattcc
     4921 ctcttccaca aagaccaaca ttacgtcatc caagacggcg aaatcgtcat cgtggacgaa
     4981 ttcaccggcc ggctgatgtc cggccgccgc tggtcggagg gtctgcatca agccgtcgaa
     5041 gccaaagaag gcgtggaaat caaacgcgaa aaccaaaccc ttgcctccat caccttccaa
     5101 aactatttcc gcctgtacac caagctctcc ggcatgaccg gcacagccga taccgaagcc
     5161 ttcgagttcc aaagcatcta caacctcgaa accgtcatca tcccgaccaa ccgccccata
     5221 caacgcaaag acttcaacga ccagattttc cgttccgccg aagaaaaatt cgaagccgtc
     5281 gtcaaagaca tcgaagaatg ccacaaacgt gggcagcccg tcctcgtcgg caccaccagc
     5341 atcgaaaact ccgaactggt ctcccacctt ctgcaaaaag ccggactgcc gcacaacgtc
     5401 ctcaacgcca aagaacacga acgcgaagcc ctgattgtcg cccaagccgg caaagtcggc
     5461 gcgattaccg ttgccaccaa tatggcggga cgcggtacgg acatcgtttt aggcggcaac
     5521 ctgaagcacc aaaccgatgc catccgtgcc gacgaagcct tgagcgacga agagaaacag
     5581 gcacaaatct ccgcactcga agacggctgg caggcagaac acgacaaagt gatggaagca
     5641 ggcggtttgc acatcatcgg tacggaacgc cacgaaagcc gccgcatcga caaccagttg
     5701 cgcggacgtt ccggccgtca gggcgacccc ggatccagcc gtttctacct ctcgtttgaa
     5761 gacccgctgc tgcgcctatt cgcactcgac cgcgccgccg ccatcctcaa ccgcctcgcc
     5821 cccgaacgcg gcgtcgccat cgaacacaac ctgctgacgc gccaaatcga aggggcgcaa
     5881 cgcaaagtcg aaggcagaaa cttcgatatg cgcaaacagg ttttggaata cgacgacgtt
     5941 gccaacgaac agcgcaaagt catttacagc cagcgcaacg aaattctgac cagcaaagac
     6001 atcagcgacc tgatgcagga aatccgttct gatgtcgtca gcgacctcgt ggatacctat
     6061 atgccgcccg acagcatgga agaacaatgg gacatcccga ctttggagaa ccgtctggct
     6121 gccgaattca gactgcacga agacatccaa tcctggctga aggcggacaa tgcgattgac
     6181 ggtcaagaca tcaaagaacg cctgatcgaa cgcatcgaaa acgaatatgc cgccaaaacc
     6241 gaactggtcg gcaagcaggc aatggccgat ttcgagcgca acgtgatgtt gcaggtcatc
     6301 gacaaccaat ggcgcgaaca cctcgccgct atggactacc tgcgccaagg catacacctg
     6361 cgcagctatg cccaaaaaaa tccgaagcag gaatataaac gtgaagcctt taccatgttc
     6421 caagacctgt ggaacggcat caaattccat attgcctccc tgcttacctc ggttcaaatc
     6481 gaacaaaacc ctgtcgcggt ggttgaagag caacccatcg gcaacatcca gtccattcat
     6541 tccgaatcgc ccgatatgga agaacttttg ggtcagtcgc aaaccgatct ggttaccgaa
     6601 gcctttaatc ccgatgggac agatttcagc cccgaagcct tggaagcgcg ggggcaaatc
     6661 gtccaccgca acgacccctg cccctgcggc agcggtttga aatacaaaca atgccacggc
     6721 aaactggctt gagcgtttga acgcaaatgc cgtctgaaca tccggtttcc ggcttcagac
     6781 ggcattttgc ctgaatcgcc gtatccgacc gacaatccga aaactcctga tttgatacca
     6841 tccgaactaa aaacaggcat ccgccccgcc gaactatgat tccatccgac ttcattgacg
     6901 aacttctggc caaaaccgat attgtcgaca tcatcgacga acaggttccg ctgaaaaaag
     6961 gcggggcgaa ctatatggcg tgttgcccgt tccacaagga aaaaacgccg tcgttttcgg
     7021 tcagtccgac caagcagttt taccactgtt tcagttgcgg ggcgcacggt tcggcgattg
     7081 gttttgtgat ggaacatcag gggctgtcgt ttccggaggc agtgcagttc cttgccgacc
     7141 gcgtgggtat gattgtgcct aaagtgcgcg ggcagaacga taatcccgaa gtccgtgccg
     7201 aacgtaagaa aaagcagcag acgctggaag aaacgacagc ggcggcggct gatttttatg
     7261 cgcaacagtt gaaattcaat ccgacggcga aggcttattt ggacaagcgc ggcttgagcg
     7321 cggaagtcat cgcgcattat ggtttgggct acgcgcccga cggctggcag cctttggcgc
     7381 aagtattcca accgtacccg aataccgcgt tggtggatac gggtatggtg attgacaatg
     7441 aggggcggta ttacgaccga ttccgtcatc ggattatgtt ccccatccgc aatccgcgcg
     7501 ggcaggtcat cggtttcggc ggcagggtgc tggacgactc caaacccaaa tatctaaact
     7561 ctcccgatac gcctttgttt gataagggga aaaaccttta cggattgtat gaagggcgtg
     7621 ctgctgtcaa ggaagtgggg cggattctgg tggtcgaagg ctatatggac gtggtcgcgc
     7681 tggcacagtt cggcgtgggc tacggcgtgg cggccttggg cacggcaaca acagcggaac
     7741 acgtcaaaat cctgatgcgt caggcggaca gtatttattt ctgtttcgac ggcgacagcg
     7801 cggggcgcaa agcggcttgg cgcgcgctgg aaaacgcgtt gccgcagttg aaagacgaca
     7861 aatcgctgca ttttttgttc ctgcccgagg aacacgaccc cgacagttac atccgtgcct
     7921 acggcaaagc gcaatttgaa gacgcgcttc tgaatcaaag caagcctttg tcggagtatt
     7981 tctgggaaca cctttcagac ggcattcatc tcaatacgca ggaaggcaag gcggaattgg
     8041 tgaaaaccag ttcgccgctt ttggcgcaga ttaccgcgcc ggcattggct tatttgttaa
     8101 aacaacggct tagcgaactg gtcggcatcg accccgacaa cctcgcgcaa ctgctaggac
     8161 aggaagcgcc gaagcggcac gtcaaacaaa aaaactacaa actgcctccg atttccgtca
     8221 aacagcccgt catgctgacg ctggtacagc ggcaaatccg cagcctcttg ataaatccgg
     8281 attgggctgc atatatagac ctgcccgatt atctggcgtt ggacggtgat ttcgcctgcc
     8341 ttgccaatct tgccgaatcg attaaaaacc atgccgccgt acccgaaacc gctcaggttt
     8401 tagagtatat gcgcggctcg ccttacgaag aaacgataac ccgaatcttc cattcaacgc
     8461 accaatcgga agaaatgaac agcagcagtg aagaagattg cgagaatttc caaatcggca
     8521 tgaaaaaact gctcaatgag ttaaaataca gccaaatcga agcattgaaa caaaaaagcc
     8581 tgcaatccgg cttaaatgaa agcgagaaaa aacttttgct gtcgttgctg accgcaaaac
     8641 aaaattgacc ggcggattcc gccacccgta aacccgttat gccgtctgaa aagcacttac
     8701 cccggctgca acaacgacac ctgcagaaca cccatcccaa aaagccttca gacggcatca
     8761 gagtacccgc tctgccacgc cttcaggtgc gttcaaacgc aaaccgtcgg caccttacca
     8821 acagaaagca gacaatgtcc agaaaccaaa atcacgaaga atatcaagac gacacccgtc
     8881 cgttaagcat tgaagagcaa cgcgcgcgcc tgcgtcagct catcatcatg ggtaaagaac
     8941 gcggctacat cacctactcc gaaatcaacg acgccctgcc agacgatatg tctgatgccg
     9001 accaaataga caatatcgtc agcatgattt ccggtttggg catccaagtt accgaacacg
     9061 cccccgatgc ggaagacata ttgttaagcg acaatgccgc cgttaccgac gatgatgccg
     9121 tcgaagaagc cgaggccgcc ctttccagtg cagattccga gttcggcaga accaccgacc
     9181 ccgtccgtat gtatatgcgc gaaatggggc aggtcgacct gctgacccgc gaagacgaaa
     9241 tcatcatcgc aaaaaaaatt gaaaacgccc tgaaaaatat ggttcaggcc atctccgcct
     9301 gcccgggatc cattgcggaa atcttagaac tcatcgaaaa aatccgcaaa gacgaaatcc
     9361 gcgtcgacga agtcgtagaa gccattatcg acccgaatga agtattgctc aacgaattgg
     9421 gcttggggca cttggaaacc acagcgcccg agaaaccttc caacgacaat tcggacgaaa
     9481 acgaagacga cgaagaatcg gaagaagatg cggatgaaat ctcggcagcc aatctcgccg
     9541 aattgaaaca aaaagtcatc ggccactttg cccaaatcga aaaagactac aaaaaaatga
     9601 ttggccgttt ggaaaaacac cacagccggc acaaagacta tctcgcctac cgcgacgcga
     9661 ttgccaacaa actgctggaa gtccgtttcg ccacccggca aatcgacagc ctcagcagca
     9721 gcctgcgcgg gaaagtagaa aacatccgca aactcgaacg cgaaatccgc gacatctgcc
     9781 tcgaccgcgt ccatatggaa cgcgactact tcatccaaaa cttcctgccc gaaatcacca
     9841 atctagaatg gattgaagaa gaaatcgcca aaggcagggt ttggagcgac gcgctcgacc
     9901 gcttccgcca cgccatcctc gaaaaacaaa ccgagttggc ggatatggaa aaagaaaccc
     9961 gcatttccat cgaagagttg aaagaaatca acaaaaatat ggtgtcaagc gaaaaagaaa
    10021 ccgcagccgc caaacaggaa atgattcagg caaacttgcg cctcgtgatt tccatcgcca
    10081 aaaaatatac caaccggggc ttacaattcc ttgatctgat tcaggaaggc aacatcggtt
    10141 tgatgaaagc ggtcgataag ttcgaatacc gcagaggcta taaattctcc acctacgcaa
    10201 cctggtggat ccgccaggca attacacgct cgattgccga tcaggcgcgt accatccgca
    10261 ttccggtaca tatgattgaa accatcaaca agatgaaccg catctcgcgc caacaccttc
    10321 aagaaaccgg cgaagaaccc gattccgcca aacttgccga actgatgcag atgcccgaag
    10381 acaaaatccg caaaatcatg aaaatcgcca aagagccgat ttcgatggaa acccccatcg
    10441 gcgacgacga cgattcgcac ttgggcgact tcatcgaaga tgccaacaat gttgcgccgg
    10501 ccgatgcggc aatgtacacc agcctgcacg aagtaaccaa agaaatcctc gaaagcctga
    10561 caccgcgtga ggcaaaagtc ctgcgtatgc gtttcggcat cgatatgaac accgaccaca
    10621 cgctggaaga agtcggcaga cagtttgacg taacgcgcga acgcatccga caaatcgagg
    10681 caaaagcact ccgcaagctg cggcatccga caagaagcga ccgtttgaga agtttcttgg
    10741 acagcgaaga cagcaagctg taaaccaaaa aaccgcaggt ttcaaatacc tgcggttttt
    10801 tcttacacaa taaacaacgc ttccacatat cccacactcc tatcccgaga cctttgcaaa
    10861 atcccccaaa atcccctaaa ttcccaccaa gacatttagg ggattttcca tgagcacctt
    10921 ctttc
//
LOCUS       DAWKOG010000055        10779 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.055, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000055 DAWKOG010000000
VERSION     DAWKOG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10779
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.055"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            123..305
                     /locus_tag="WJK66_001062"
     CDS             123..305
                     /locus_tag="WJK66_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578786.1"
                     /translation="MFWYVIGFCSFVVALLSLWVNVGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPDTEEKQQ"
     gene            complement(367..1368)
                     /locus_tag="WJK66_001063"
     CDS             complement(367..1368)
                     /locus_tag="WJK66_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEZ7578787.1"
                     /translation="MIDGQTTEPKQKTRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIRDSVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRRILNAFEHEINV"
     gene            complement(1444..5277)
                     /locus_tag="WJK66_001064"
     CDS             complement(1444..5277)
                     /locus_tag="WJK66_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HEZ7578788.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVERFDSSFDETSQKRHQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEVAPLVRALIELDLVIIPRGGGTGYTGGAIPLDAMSAVIN
                     TEKLDKHRGVEFVELAGLDGRHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWQMVNPQGEWLRIERVRHNFGKIHDEETAIFD
                     VHTLDSDGLNVVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITSVAF
                     VLHKMPKYTRTVCMEFFGTVATATPSIVEIRDFLLAHESVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAISPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKARWDWLLANLDTPLADYKARYGAA
                     VHAAPEAKDNESCFTAFRDFRLRVSVKADVMKPLSEIFSGKTDTKIIEGLGKIHAKTV
                     RGRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLNGVISGEHGI
                     GITKLEFLSDEEMQPFWDYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSVKHFEELADIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     SMGMAFLNATGPKTIKTLRAAMIQTGFPAQNFAYKIGKLLPVGTKKQKAEPKATVGKA
                     PIKEQIIHFINRPLPKNVPAKTPRSLLGIEDDKSIPIIRNPASPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLNGVKGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYADDNNMPTDYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            5538..5634
                     /gene="ffs"
                     /locus_tag="WJK66_001065"
     ncRNA           5538..5634
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="WJK66_001065"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5801..7492
                     /gene="dld"
                     /locus_tag="WJK66_001066"
     CDS             5801..7492
                     /gene="dld"
                     /locus_tag="WJK66_001066"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEZ7578789.1"
                     /translation="MSASQLLSRLTQTVGEKYIITDPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKASFLPKHFSDKAMQFVSKYLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFAHHSGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPPEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGVGKTSKKKNWAE"
     gene            7624..7731
                     /locus_tag="WJK66_001067"
                     /pseudo
     CDS             7624..7731
                     /locus_tag="WJK66_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7787..8158
                     /locus_tag="WJK66_001068"
     CDS             7787..8158
                     /locus_tag="WJK66_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEZ7578790.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            8313..8861
                     /locus_tag="WJK66_001069"
     CDS             8313..8861
                     /locus_tag="WJK66_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ7578791.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDEELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLGQTEINPELQAYA"
     gene            8996..10087
                     /locus_tag="WJK66_001070"
     CDS             8996..10087
                     /locus_tag="WJK66_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ7578792.1"
                     /translation="MNAQTLIANVAEFVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNRAVKDK
                     YLADILQGKVLAGTGMSNTVKHLADIEKHNLQAERVEGGYTVNGALPWVSNIGEDHIW
                     ANTAQIGGGYVMFITGGQWEGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANVVNAEVNRYLDDGYDSLKTRLD
                     GAWAETERLAGLAWSGTPDNLTTLKLREAAAVLTLAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
     gene            10545..10715
                     /locus_tag="WJK66_001071"
     CDS             10545..10715
                     /locus_tag="WJK66_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEZ7578793.1"
                     /translation="MAQYMCGPCGWIYDEEHGDPEHGIAPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
BASE COUNT         2510 a         2956 c         2908 g         2405 t
ORIGIN      
        1 atttgtactg tcttcggctc gccgccttgt ccttatttaa atttaattca ctatatttgc
       61 ccgtgcgttt gtgataaatt cggcactgta tttgatatga ttttaacaaa tcgggagggg
      121 caatgttttg gtatgtaatc ggcttttgct cctttgtcgt cgcgctgctg tcgctgtggg
      181 tcaatgtcgg cgcattcggt atgcaggaag acgatacccc gcaatcggat tatgaaaaac
      241 gtttgggctt gggtgcgaag ctgaaaaaca aaaatacgcc ggatacggaa gaaaagcagc
      301 aataaagata tgaaatgccg tctgaaagat tttcagacgg catttgcttt gaagcatggg
      361 agacagtcaa acatttatct catgctcaaa tgcgttcagg attcggcgga cttcatccgc
      421 atccttaacc gttcggacgc ggtcaaacag cgtttgcgcc gggtcgaacg tttttttcat
      481 catgcccaac cattgcttaa gacgggcgat cgggtattta ttgtttgcct cttttgtcag
      541 gcacagctcg aaaaactgcc gtatccattt ggaaacttcg gcaaaatccg tgtctttgac
      601 cggcccgccg ttctcgtatt gcttgatttg ccgcgccaaa tcggggcgga tgaccgcgcc
      661 gcgaccgagc atcacgctgt tgcagccgct gattgttttg atgccgatat agtcttgcag
      721 gctgaaaacg tcgccgttgg cggtaacggg aatattgacg ctgtctcgga ttttccttat
      781 ccattcccaa tgcgccggcg gttcgtaacc ctcggctttg gtacgcgcgt gtacggtcag
      841 tccgcatgcg cccccttcgg caatcgcaca ggcgcattcc aaagcccggc ttttgtcttc
      901 gtaaccgagc cgcatttttg cggtgagcgg aatatgtgcg ggcaaacgtc cgcgcagcgt
      961 tttgacgatg tggaatatca gttccggctc ttttaaaagg attgcgccgc ctttgtgttt
     1021 gttgacggtg ggcgcggggc agccgaagtt caaatcgatt ttgttcgcac cgaagcggac
     1081 ggcttccagc gcattcgccg ccatattgtc cgcatcgctg cccaaaagtt ggacggtgca
     1141 gggcgtgccg gaaaacgttt tgtttccgtt ggcgatttcg gggacatatt ttaaccatat
     1201 ggatcgggaa tgcacggtat gggtaatgcg tacaaattcg ctgacgcatt cgtcgtagcc
     1261 gccaatacgc gtcagcaggt cgcgcatcac gtcatcgacc agaccctgca taggggcaag
     1321 gatgattcgg gttttttgtt tgggttcggt tgtttgtccg tcaatcatga tttgggtagg
     1381 tattgggttt aaacaatgcc gtctgaagac ttcggcgttc agacggcatt tccgtgtccg
     1441 ttgttacagc aatacttttt ccacgccgcc gttattggct tttttcacaa actcgtctag
     1501 ccagttttcg ccgaggatgt gtttcgccat ttcgataacg atgtagtcgg tgggcatatt
     1561 gttgtcgtcg gcgtagcggc tcaagccttg cagacacgcc ggacaggagg tcagcatttt
     1621 gacgggttcg ccctgcggca gctctttgag gtttttctca atttcctctt gtttgcggaa
     1681 tttgacttgg gtggcgatgt cggggcgttt gacggcaaac atgccggatt cgccgcagca
     1741 gcggtcgctt aagacgactt tctgccccat caggctgctg gccatttggg tggcgttcat
     1801 ggttttgatg ggcgtgtggc aggggtcgtg gtagaggtat tgctgacctt tcacgccgtt
     1861 cagtttcacg cctttttcga gcaggtattc gtggatgtcg atgatgcggc agccggggaa
     1921 gatttcttcg aagcggtatt tttcgagctg gtcgtagcag gtaccgcaac tgacgacgac
     1981 ggttttgatg tcgaggtagt tgagggtgtt tgccatacgg tggaaagcca cgcggttgtt
     2041 ggtgctcatt tcttcggctt ttgccttatt gccgccggcg tcctgcggat agccgcaaca
     2101 catatagccg ggcggcagga cggtttgcac gccgacgtgc cagagcatgg cttggacggc
     2161 gagtccgatt tggctgaaca ggcgttccga accacagccg ggaaagtaaa acacggcttc
     2221 ggcatcttcc ggagaagccg gattgcggat gatggggatg cttttgtcgt cttcgatgcc
     2281 caataaggag cgcggtgttt tggcaggcac gtttttgggc agcggacggt tgatgaagtg
     2341 gataatttgt tctttaatcg gggctttgcc gacggttgcc ttgggttcgg ctttttgctt
     2401 tttcgtgccg acgggcagca gtttgccgat tttgtaggcg aaattctgcg cggggaagcc
     2461 ggtttgtatc atcgcggcac gtagggtttt aatggttttc gggccggtcg cgtttaggaa
     2521 tgccataccc attgaagctg ccggcgcaaa gcgtttgtgg ccggaatcgg caaggtagtt
     2581 gcggacggct acggttacgt cgccgaagtc gatattgacg gggcagggtt tgacgcagcg
     2641 gtggcacacg gtgcagtggt cgccgatgtc tgccagttct tcaaaatgtt tgacggaaac
     2701 gccgcgacgg gtttgttctt cgtataaaaa ggcttcggtc aagagcccca cgccgaggat
     2761 tttgttgcgc gggctgtaca gcaggttggc acgcggaacg tgggtggagc agacgggttt
     2821 gcatttgccg cagcgcaggc agtctttgac ggaatcggca atcgtgccga ggtctgattt
     2881 ttccataatc agcgattccg cgcccaaaag ctcgaaagac ggcgtatagg cgttgcgcag
     2941 gtccgagcct ttcatcagtt tgtggcggtt gaaggtgtgt ttgggatcga cttggttttt
     3001 gtagtcccaa aacggctgca tttcttcgtc gcttaaaaat tcgagcttgg taatgccgat
     3061 gccgtgttcg ccggaaatca cgccgttaag cgaacgggcg attttcataa tgcgttccac
     3121 agaacggtat gccgtctgaa gcatttcggc atcgtctgaa ttgacgggga tattggtgtg
     3181 gacattgccg tcgccggcgt gcatatgcag ggcgacaaag actctgccgc gcacggtttt
     3241 ggcgtggatt ttgcccaagc cttcgatgat tttggtgtcg gttttgccgc tgaagatttc
     3301 agaaagcggc ttcattacgt ctgctttgac agacacgcgc aggcggaaat cgcggaatgc
     3361 ggtaaagcag ctttcattgt ctttggcttc gggcgcggcg tggacggctg cgccgtagcg
     3421 ggccttgtag tcggcaagcg gcgtgtcgag attggcgagc aaccagtccc aacgcgcttt
     3481 gacggcggaa acgtgggcca gggcgtgttt gccgcgttcg cccaacagtt cggcggtcgg
     3541 caggtcggtt cccattttgt cgatggggag tttgccggac agatattgct ccaaggcggc
     3601 acagagtttg agtttgtttt gaatggaaag ctcaatgttg atgcgttcga tgccgtctga
     3661 atactcgccg agcctttcga gtgggatgac cacgtcttcg ttgattttaa aggcgttggt
     3721 gtgtttggcg atggcggcgg tgcggctgcg gtcgagccag aaggttttgc gggcttcggg
     3781 agatatggcg ataaagcctt cgccgtcgcg ggcgcgggca agttcgcaga tgtgttcggc
     3841 ggctgcctct acggcggctt cgtcgtctga aaccacgtcc gccagcaaaa ccattttcgg
     3901 ccgtcccttg cccgccgctt tggtggcgta gccgacggcg cggacataac gccagtccaa
     3961 atgttccaaa cctgccagcc gtacgctttc atgggcaagc aaaaagtcgc ggatttcgac
     4021 aatagatggc gtggcggtgg caaccgtgcc gaaaaactcc atacacacgg tgcgcgtgta
     4081 tttcggcatt ttatgcaaca cgaaggcaac gctggtgatg atgccgtccg tgccttcttt
     4141 ctgcacgccg ggcaggccgc tcaagaattt gtcggtaacg tctttgccca aaccgacttt
     4201 gcggaatttg tgaccgggaa tttccaagcg ttcggttttg acgacattga ggccgtctga
     4261 atccagtgtg tgaacgtcaa atattgcggt ttcttcgtcg tggattttgc cgaaattgtg
     4321 gcgcacgcgt tcgatacgca gccattcgcc ttgagggttg accatttgcc agtaggcgag
     4381 gttgtccaac gccgtccccc acagcacggc ttttttaccg cccgcgttca tcgctacatt
     4441 accgcccacg caggacgcgt cggcagaagt cggatcgacg gcgaacacca aacctgcctg
     4501 atgcgcggtt tcttccactc gccgcgtaac cacgcccgcg ccgcaccgga taatcggatg
     4561 tctgccatcc aagcccgcca gttcgacaaa ttccacgccg cgatgtttgt ccagcttttc
     4621 cgtattgatg accgcgctca ttgcgtccaa aggaatcgcg ccgccggtat aacccgtacc
     4681 gccgccgcgc gggataataa ccaagtccaa ctcaattaag gcgcgcacca aaggcgcgac
     4741 ttccgcctcc gtgtcgggat tgacgacgac aaacggatat tcgacgcgcc agtcggttgc
     4801 atcggtaaca tgcgttaccc gcgccagccc gtcgaacata atattgtgcg gcttggtaat
     4861 tttgcttaag cgttccaaaa tttgatggcg tttttggctg gtttcatcaa aactgctatc
     4921 aaaacgctcg actgcctttt ccgctgccgc aaccaataca tccacttgcc gattatcgtc
     4981 gcggcgtttg cggatttcgt tcaaacggtg gcgcatttcc cgcaccagcg cggcgcggcg
     5041 tttcgggtgc tccaacaagt catcgaccag atatggattg cgcacaacta cccaaatatc
     5101 gcccaacact tcaaacagca tccgtgccga acgtccggtt ttacgctgtc cgcgcaagtc
     5161 ttgcagaatc tgccacgctt cgtcgcccag caggcggatg acgatttcgc ggtcggtata
     5221 agaagtatag ttatagggaa tttctcgaat acgctgaggg gcagtagtcg tggtcatggt
     5281 gtgtccgtgc ttggcagttt ttatttgaat gccgtctgaa aattcggttt caggcgagtt
     5341 tgaaaaggcg ataatgtagt tcaatttttg atatttttca atgccgaaac aggaaatacc
     5401 cttatagaaa caaaataacg ctttgagaaa ttgttgacaa tattatcaaa tataaatttt
     5461 aggtggtgtt tccggcgtat gaccgtatca aaacggttga gtttttcaga aaacccgtta
     5521 taatgccgtt tctctgaggc gggtctcccc gcatggcaaa tcggaacacc gggtcagggg
     5581 cggaagccag cagcccactc cggtgcgcca gtgccggggg tttggcccgc cgccctattt
     5641 gaaacgccgg agctttgatg ttccggcgtt cccgtatcgc accgttgccg tctgaaagcc
     5701 gttcagacgg cattttcttt actttgccat aacgtataac tacaatgtga aaacaattcc
     5761 aatgtgcaat gcacctgccg acaattttaa ggaaactgcc atgagcgcat cacaattact
     5821 gtcaagactg acccaaaccg taggcgaaaa atacatcatt accgaccccg cgaaaaccga
     5881 acaataccgc caaggttacc gcttcggcga gggtaaggcg ttggcggtgg ttcgccccgg
     5941 aagcattctg gaaatgtgga aaattctgca ggcgtgcgtc gaagcagacg tgattgtgat
     6001 tacgcaggcg gcgaataccg gactgaccgg cggttcgacc cccgacggca acgattacga
     6061 ccgcgacatc gtgattgtga acaccatgcg gatgaacatc atccaaacca tcaacaacaa
     6121 cgaacaagtc gtctgcctgc ccggctcgac cctgaaccag cttgaattgc tgctgaaacc
     6181 cttgggacgc gaaccgcatt cggttatcgg ctcttcctgc atcggtgcgt ccgtcttggg
     6241 cggcgtgtgc aacaactccg gcggcgcatt ggtacagcgc ggcccggcct acaccgaaat
     6301 ggcactgttc gcccaaatca acgaagaagg caggttggaa ctggtcaacc acttgggcat
     6361 agacttgggc gacacgcccg aagaaatcct gaccaacctg caaggtcatc attatcaaaa
     6421 caaagacatc aaacaagacg cgggcaaagg acacgaccac gcctattgcg aacacgtccg
     6481 ccaagtggac gaaccgaccg ccgcgcgttt caatgccgac cccgcccgcc attacgaagc
     6541 ctcgggctgc gcgggcaaac tgatggtttt tgccgtccgt ttggacacct tcccgcaaga
     6601 aaaacaaacc gccgtgttct acatcggcac gaacgacatc aacgagctga ccgacatccg
     6661 ccgcgccgcc ttgggcgaat ttgaaagcct gcccgtttcc ggcgaataca tccaccgcca
     6721 cgctttcgat attgccgacg tgtacggcaa agacacgttt tacgtcatca aaaaattcgg
     6781 tacgcaccaa ctgccgaaat tatttgactt gaaggcgcgc gtggacaggt tcggcaaaaa
     6841 agccagcttc ctgcccaaac atttttccga caaggccatg cagttcgtca gcaaatacct
     6901 gcccgaccac ctgcccaaat ccatgcgcga ttaccgcgac aaatacgaac accacctgat
     6961 tctgaaaatg ggcgggaaag gcgtggatga ggcgcgcgcg ttcttaaaag aatattttgc
     7021 acaccacagc ggcgcgtttt tcgagtgcaa cgccgaagaa acccaagccg cgatgctgca
     7081 ccgtttcgcc gtcgcctccg ccgccatccg ctaccgcgcc gtgcatgatg acgaagtgga
     7141 agacttggtc gcgcttgaca tcgccctgcg ccgcgacgac cgcgactggt ttgaaaaact
     7201 gccgcccgaa atcgacaata aaatcatcca caaactctac tacggacatt tcatgtgcca
     7261 cgttttccat caggattaca tcatcaaaaa aggcaacgac tgcatggcgt tggaacacga
     7321 aatgctgcat cttttagacc aacgcggcgc gcaatatccc gccgaacaca acgtcggtca
     7381 tttgtatgaa gccaaacccg cgctcaaaca gttttaccgc aaacttgacc cgaccaacag
     7441 cttcaacccg ggcgtaggca aaaccagcaa aaagaaaaac tgggcggaat aagcgtgtcc
     7501 gctttgaagg caggcaatgc cgtctgaagg caaaacgccg ttcagacggc attgtcgggt
     7561 ttgaagttcc ccgtcgggta atcgctttca aaccgtatgc cgacacacgg gcgcgcccct
     7621 cccaaatata gtggattaaa tttaaatcag gacaaggcaa cgaagccgca gacagtacag
     7681 atagtacggc aaggcgaggt aacgccgtac tggtttaaat ttaatccact ataaagtctg
     7741 atcctacctt cccaaagggc agggtttcaa ccgaaaagga aacacgatga aaccatacaa
     7801 aatctacacc catcccgccc tgccgcccca agccgtcaaa caaggctggt catggccggg
     7861 gctcttgttc ggcacgctgt gggcgtgttt caaaagaatg tggggtttgg ggctggggct
     7921 gacgggcgcg atattcgtgc tggctgtgtt tgcccaactg gtttacggcg acacgcccgc
     7981 cacagactcg gcgtttaacg tattgggctt ggcggtttcc gtctggttcg gcgcaaaggg
     8041 caacagcctt tatgcacgcc accttttatc gcgcggctat accgaattgc ccgaaacggt
     8101 tgaggcagcc aatccccaag ccgcattggc gcaatatttc gggcgcgggg gccgataagc
     8161 gcgttttctt ttccatcgtg ccgtctgaaa aatgttcaga cggcatttct tataaccttt
     8221 cgtttgcagc acataaccaa tcagtctttc ctattccgtt ccgaaatgca taccatgcgc
     8281 ccgtacccaa tatttgcgaa caaggaaaga aaatggcacg tttaaccgta cacaccctcg
     8341 aaaccgcccc cgaagccgcc aaagcgcgcg tcgaggcggt acttcaaaac aacggcttta
     8401 tccccaacct tatcggcgta ttatcaaacg cccccgaagc cttggcgttt taccaagaag
     8461 tcggcaagct caacgccgcc aacagcctga ccgccggcga agtcgaagta atccagatta
     8521 ttgccgcccg caccaaccaa tgcggcttct gcgtggcagg gcacaccaaa ctcgcaaccc
     8581 tgaaaaaact cctttccgaa caatccgtca aagccgcgcg cgctttggcg gcaggcgaat
     8641 ttgacgatgc taaactcggc gcgctcgccg cctttaccca agccgtaatg gcaaaaaaag
     8701 gcgcggtatc cgacgaggaa ctcaaagcat tttttgatgc gggctacaac cagcagcagg
     8761 cagtcgaagt cgtgatgggc gtagccttgg caactttgtg caactacgtc aacaacctcg
     8821 gacaaaccga aatcaacccc gaattgcagg cttacgcctg atacggcaaa ccgcccgaat
     8881 atcgggcggt ttttcaaaaa caccccctca aacaaaaaca agccgcctaa gcggcaggga
     8941 agcagccgcg catcaggcgc gcccccgatt tccccgcaaa ccttaaggaa caaagatgaa
     9001 cgcccaaacc ctgattgcca acgttgccga attcgtcaaa accaagctca aacccatagt
     9061 ggacgacatc gaccgcaaag gatactaccc cgaagcattt atgcgcgaac tcggcgcaat
     9121 cggtggattc ggcgcagtcg gtacagaagc cgaaggcggc aacggcttgg gtttggcaac
     9181 gcaaatcgcc gtattgcgcg aaatcggcaa agaatgcggc gcaacctctt tcagtgcgtg
     9241 gtgtcaggcg gcttgcgcgt ggtatctgca ccaaacgccc aaccgggccg tcaaagacaa
     9301 ataccttgcc gacatcctgc aaggcaaagt attggcgggt acgggcatgt ccaataccgt
     9361 caaacacctt gccgacatcg aaaaacacaa cctccaagcc gagcgcgtgg agggcggcta
     9421 cacagtcaac ggcgcgctgc cgtgggtgtc caacatcggc gaagaccaca tttgggcgaa
     9481 taccgcccaa atcggcggcg gctacgtcat gttcatcaca ggcggacaat gggaaggcgt
     9541 aagcctgcaa aactgccccg aattttgcgc cctcgaaggc acacgcacct tcagcctgaa
     9601 cttcaaagac gtatttatcc ccgacgaaga catcatcgcc gcgcccgaac agtttgccga
     9661 ctacatccaa agcatcaaag caggctttat cctcctgcaa atcggcatcg gcgcgggcgt
     9721 gatagacggc agcctcggca tcatccgcct cgccaacgtc gtcaacgccg aagtcaaccg
     9781 ctatctcgac gacggctacg acagcctcaa aacaaggctg gacggcgcgt gggcagaaac
     9841 cgaacggctc gccggattgg cttggagcgg cacgcccgac aaccttacca ccctcaagct
     9901 gcgcgaagcc gccgccgtac tgacacttgc cgccgcccaa tccgccgccc tgcattccgg
     9961 cgcaaaaggc tacctgatgc ggagtcccgc ccaaaggcgc gtccgcgaag caatgtttgt
    10021 cgccatcgta actcccgcga tcaaacacct ccgcaaagaa atagcggcaa tcgaagccgc
    10081 aaaataaaca ggcggaaaaa accgaaaccc ccatcgcgtc attcccgcga aagcgggaat
    10141 ccagccccca acgtaacagg aatccatcgg aaaaaacaga aaccctcaaa ccgtcattcc
    10201 cgcaaaagcg ggaatccagc cccccctaac gcaacaggaa ttcatcgaaa aaaccgaaac
    10261 ctccaaaccg tcattcccac ggaagtggga atccagtaat cgaaaaacca caggaatcta
    10321 tcgaaaaaaa accaacccca aaaaaaccgg gcggataccg caccattctc ccaaaccctg
    10381 atttatagcg gatgaacaaa aatcagggca aggcggcgaa gccgcagaca gtacaaatag
    10441 tacgggtgct tcagtacttt agagaatcgt tctcttcgag ctaaggcgag gcaacgccgt
    10501 accggttttt gttaacccac tatacaaaaa acaaggaaac acaaatggcg caatatatgt
    10561 gcggcccctg cggctggatt tacgatgaag aacacggcga ccccgaacac ggcatcgccc
    10621 ccgggacaaa gtttgaagac atccccgacg actggaaatg ccccgaatgc ggcgtgggca
    10681 aagaagattt ctacctgttg gatttcgtga tatagcggca gccgcaccct tagggaaaac
    10741 cccgtcatcc ggcgttccgc ctgcggcggc agggaacac
//
LOCUS       DAWKOG010000056        10415 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.056, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000056 DAWKOG010000000
VERSION     DAWKOG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10415
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.056"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(26..349)
                     /locus_tag="WJK66_001072"
     CDS             complement(26..349)
                     /locus_tag="WJK66_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ7578794.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IVPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(337..576)
                     /locus_tag="WJK66_001073"
     CDS             complement(337..576)
                     /locus_tag="WJK66_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HEZ7578795.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFSEIENEADKEWIW"
     gene            complement(699..1496)
                     /gene="truA"
                     /locus_tag="WJK66_001074"
     CDS             complement(699..1496)
                     /gene="truA"
                     /locus_tag="WJK66_001074"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEZ7578796.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALETALAQI
                     AGEAVSTTVAGRTDTGVHATAQVVHFDTTAARPQQAWIRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIEPMRQAAAYLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSAGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPQIPEWL"
     gene            complement(2461..2646)
                     /locus_tag="WJK66_001075"
     CDS             complement(2461..2646)
                     /locus_tag="WJK66_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578797.1"
                     /translation="MPEQARARPIQTAMTLCRRFGAGWRHTRRLPRFISHRSSETRNP
                     PGPTCGMKRLSWREVFY"
     gene            complement(2757..3449)
                     /locus_tag="WJK66_001076"
     CDS             complement(2757..3449)
                     /locus_tag="WJK66_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ7578798.1"
                     /translation="MTAFVHTLSDGMELTVEIKRRAKKNLIIRPAGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRQTLAKTPPPQTAENRLPESILFHGRQLALTAHQDTQILLMP
                     SEIRVPEGAPEKQLALLRDFLERQAHSYLIPRLERHARTTQLFPAPSSLTSAKTFWGV
                     CRKTTGIRFNWRLVGAPEYVADYVCIHELCHLAHADHSRAFWALTRRFAPYTPEAKQW
                     LKIHGRELFALG"
     gene            complement(3446..4189)
                     /locus_tag="WJK66_001077"
     CDS             complement(3446..4189)
                     /locus_tag="WJK66_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ7578799.1"
                     /translation="MLIIRNLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPHASGSEAELM
                     GKCEHLIETQQPLISGAGPFAAKMPSETA"
     gene            complement(4218..4781)
                     /gene="gmhB"
                     /locus_tag="WJK66_001078"
     CDS             complement(4218..4781)
                     /gene="gmhB"
                     /locus_tag="WJK66_001078"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEZ7578800.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGY
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADNCNCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            complement(4778..5350)
                     /locus_tag="WJK66_001079"
     CDS             complement(4778..5350)
                     /locus_tag="WJK66_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ7578801.1"
                     /translation="MNYKIYDGAKIIKKENLSIGDYSQIDDFVFFNCGEKSILGSFVH
                     ISSFTSIIGGGEFYMDHFSGLSAGCRIITGSDDFMGGGGLTNPTVPSKYTNVKKSSVR
                     IGKHAILGTNVTVLPGVTIGDGVAVGAGAIVRKNLEPWTIYVGVDCKPLKRRSQDKIL
                     ELEKHLVAEFNLDLCPIIPSPTRPNPKETR"
     gene            5520..7172
                     /locus_tag="WJK66_001080"
     CDS             5520..7172
                     /locus_tag="WJK66_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ7578802.1"
                     /translation="MVAYVFLFLFVTAALVLIIRSHYRWTYFFASALFVFLAGGMLML
                     TAQWQRALNFASVWFVVLILFHRLKIHYYKQPLLISDFLLIADWRNWETLFHYKEAVI
                     GMAGLLALAAYAVFGWSGADSLDVPWRWAGAVLFAAAFVSMRHFSKHPGAVKTWLDSL
                     PDDGRDVFLNLPMSCRAVFFQVPVFEGDGEAFARQMPSETRPCGMSDEKPDIVVTLME
                     STLDPHCFDFAAAKIPDLKMFGRQEDTVFSSPLRVHTFGGATWKSEFAFLAGVPSTDF
                     GALASGVFYSVVPHLQTGFVRNLREHGYFCVALSPFTKGNYNAKAAYDHFGFNLMFQP
                     QDLGYPAPMGKNLWHISSEEMMQYARMILEKRHPDLENVRQPMFVYVLTMKEHGPYRT
                     DTDNVFDLDAPDLNAKTVSALNDYIGRIADLDKAVESFDRYLHERGKPFVFGYFGDHQ
                     VPFEGVSVRKKWDYAQPDYVTQFAVRSNIAGGFVQRQNFLDLAFAGGVLMEAAGLEAK
                     DGFMRANMAMRGLCGGGLEDCPNWELVGNYRNYLYDVLKIAR"
     gene            complement(7242..8483)
                     /locus_tag="WJK66_001081"
     CDS             complement(7242..8483)
                     /locus_tag="WJK66_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HEZ7578803.1"
                     /translation="MPTKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRSWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGLGSAAGGNVSLTVGQLVFFGILAFCVWASARLVDRFTSVLIGGMVLTF
                     IWATGGLIADAKLPVLFDTQAPTGTNYWIYVATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSVSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIVNIAAQVLSQMELVPVFKG"
     gene            8635..9363
                     /gene="ubiG"
                     /locus_tag="WJK66_001082"
     CDS             8635..9363
                     /gene="ubiG"
                     /locus_tag="WJK66_001082"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEZ7578804.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLCGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACANLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            9400..10317
                     /gene="thrB"
                     /locus_tag="WJK66_001083"
     CDS             9400..10317
                     /gene="thrB"
                     /locus_tag="WJK66_001083"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEZ7578805.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYNAWWTEACARLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKVFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
BASE COUNT         2220 a         2446 c         3124 g         2625 t
ORIGIN      
        1 aacacaatcc attcagacta ttcaatcagg caaacatctc ctgcaatact gcaaacagtt
       61 tttcagccgt actgttgtct aaatttccaa gatgtttgac caatccggct ttatccacag
      121 ccctaatctg ttcgggcaaa agcaaaccgt ctttatcctg aaagcggaca ttgacgcgga
      181 acggggcagg acggcttccg ctcgtcatag gaacgatcag cacagtcttg agatagttgt
      241 gtatttcagg aggagatact acgacacaag gacgtgtctt tttgatttcg cttcctacag
      301 tcgggtctaa ggagaccaga tagattccgc cgcgtactac catatccatt ctttatccgc
      361 ttcgttttca atttcggaaa aaaaatgctc ctgctcggtt tcgacaagca ttgcggcagc
      421 ttctgcccat cctctgcgaa cggtaggaca gcttaaaata atattgccct tttcaactgt
      481 aacagccaag ctgtccactg cccctatttg acccaataat gatttgggca gaatcactcc
      541 ttgcgagttt cccattttgc gtatgttgag aatcatatgc gtaccatatt taccagttta
      601 tgtaataaca atgttattac cttgatgagg tagtgtcaat attgaaaaag attgcctgac
      661 agtttgtccg atttcaaaat ctccgcgaca agaatgtttt aaagccattc ggggatttgg
      721 gggcggatga tgccgtatgc ctcgggatag tcgacgccgg tcaggtaaag cccgtcgggc
      781 atgaaggtcg gtggggcttt gaggcggctg cgttcttgaa tgagggcggc gaagccttcg
      841 acgctgagcc tgccgctgcc gacataaacg agcgcgccca tgatgttgcg taccatatgg
      901 tgcaaaaagg cgttgccgtg caaatcgagg cggacgagtc ctgcgctttg ggtaaggtcg
      961 gcgcggtaaa tggttttgac gggggatttt gcttggcact cggcggcgcg gaagctggag
     1021 aagtcttgtt cgccaatcaa ataggcggca gcctgccgca tcggttcgat gtcgagtttg
     1081 aggtgtgtcc agcctgccct gtttttgagc aggggggaac ggacgggggc ggattcgagc
     1141 aggtagcggt agtgccgtcc gtatgcgtca aatcgtgcat gaaattcggg ggcgacctgt
     1201 cgggcgtgca aaacggcaat gccttcgggc aggtgggcat ttacgccgcg tatccatgcc
     1261 tgttggggac gggcggcagt tgtgtcgaag tggacgactt gggcggtggc atgcacgccg
     1321 gtgtcggtcc tgccggcaac ggtggtggaa accgcttccc ctgctatttg ggcgagcgcg
     1381 gtttccaatg ccgcctgaac ggtcggtacg ccgtcagcct gtttctgcca gccgtaaaag
     1441 cggctgccgt cataggatag ggttattgcc cagcgttgtt tttgtgcggt atccatcgga
     1501 tttgggattc ggataaatgt tcagactgca ttgtatcgca gattttgcag ggaaacggca
     1561 aacgcccagg gcgagcggcg ttgtttgggg agttgttggg gggggggatg cagttgctac
     1621 gaatcgctat cctgtgaatt caccctgtca ggagtgcccg aatcgtcatt cccgcgcagg
     1681 cgggaatcta ggacgtaaaa tctaaagaaa ccattttata gtggattaac aaaaaccagt
     1741 acagcgttgc ctcgccttag ctcaaagaga acgattctct aaggtgctga agcatcaagt
     1801 gaatcggttc cgtactatct gtactgtctg cggcttcgtc gccttgtcct gatttttgtt
     1861 aatccactat atccgataag tttccgcacc gacaaaacta gattcccact ttcgtgggaa
     1921 tgacgggatg caggttcgtg ggaatgacgc gaacagaaac ctcaaatccc gtcattcccg
     1981 cgcaggcggg aatctagacc ttagaacaac agcaatattc aaagattatc tgaaagtctg
     2041 ggattctgga ttcccacttt cgtgggaatg acggaatgta ggttcgtagg aatgacggaa
     2101 tgtaggttcg taggaatgac gggatgtagg tttccgtatg gatggattcg tcattcccgc
     2161 gcaggcggga atctaggtct gtcggtgcgg aaacttatcg ggtaaaacgg tttcttcaga
     2221 ttttacgttc tggattccca ctttcgtggg aatgacgcgg tgcaggtttt cttaaccccg
     2281 cgttctagat tcccactttc gtaggaatga cggcggtaga tttggcagat gcggcggatt
     2341 cggcaggtct caacccatcc tacaatccac cctgaccgcc ttttacgaat ccgccgccat
     2401 cctgggaaac gcaaaaaaat gccgtccgaa aacctttcag acggcatttt cgcgggcaaa
     2461 tcagtaaaag acttcgcgcc agcttaagcg tttcataccg cacgtcggac cgggcgggtt
     2521 tcgggtttcg gaagagcggt gtgaaatgaa acgcggcaga cggcgcgtgt gccgccatcc
     2581 tgccccgaac cggcggcaaa gcgtcatcgc ggtttgaatc gggcgggcgc gtgcctgttc
     2641 cggcacggat gccgtctgaa agcggcggca gcggtatgtg tcgccggctg tgatgctgct
     2701 ttccggatgt ccgtttggga ggaggtctga attggcggat gcctgcccgg gccgcgtcag
     2761 cctaaggcga aaagttccct gccgtggatt ttgagccact gtttcgcttc gggcgtgtag
     2821 ggtgcgaagc ggcgggtcag tgcccagaac gcgcggctgt ggtcggcgtg ggcgaggtgg
     2881 cagagttcgt gtatgcagac atagtcggca acgtattccg gtgcgccgac cagccgccag
     2941 ttgaagcgta tgcctgtggt tttgcggcac acgccccaga aggttttggc agaggtcagc
     3001 gaggaggggg cggggaacag ttgtgtggtg cgggcgtggc gttcgaggcg gggaatcagg
     3061 taactgtgcg cctgccgttc caaaaagtcc cgcagcagcg caagctgttt ttcgggtgcg
     3121 ccttcgggaa cacggatttc agacggcatc agcaggattt gcgtgtcttg atgggcggtg
     3181 agggcaagct gtctgccgtg gaagaggatg gattcgggca gccggttttc ggcagtttgc
     3241 ggcggcggtg ttttcgccag tgtttgccgc aggacggctt cgttttcata cagccagcgg
     3301 tttagagcgg agacggagaa gcagggtggg acgctgatgc ggacggtatg tgtgccggcg
     3361 gggcggataa tcaggttttt cttggcacgg cgcttgattt cgacggtcag ttccatgccg
     3421 tctgaaaggg tgtggacaaa ggcggtcatg cggtttcaga cggcattttg gcggcaaacg
     3481 ggcctgcgcc ggaaatgagc ggctgctgcg tttcgatgag gtgttcgcat tttcccatca
     3541 attcggcttc gctgccgctt gcgtgcggga tggtcggaca gatgacgacg gtgatttccc
     3601 ccggatattt cagaaaggag tttttcggcc aaaattcgcc gctgttgagg gcgacgggga
     3661 cgatgtccat ctcaaacatt ttcgccatgc gcgcgccgcc gagtttgtat ttgccgcgtt
     3721 ttccgggcgc aaggcgtgtg ccttcgggga aaatggtaat ccaatagcct tcgtttttgc
     3781 gcgccaaccc ctgttttatg agctgctcgt tggcttcgcg gcggttgttg cggtctatgc
     3841 ctatggtttt gaccagtttc aagccccagc cgaaaaaggg gattttgaac aactcgcgtt
     3901 tggcaacgta aacctgcggc ggaaaaatgt cctgaagggc gagcgtttcc cagccgcttt
     3961 ggtgtttggc gcagatgacg gcggggcggt cggggatgtt ttccgcgccg atgatgcggt
     4021 atttgagccc gacgatgtgt ttgagcgaga ggttgaggat tttgacccag acccgcgcca
     4081 tcttgtgcgc cccgtctcgg aaaggcgagg cgagcagcat aaagggaaag aggaaaatca
     4141 gggtggaaca gagtatcagc cagtaaatca ggttgcggat gatgagcatg gttttgcctt
     4201 gtcggaatgc ggtatgttca gtcggcttgc ggtgcggcgt tttcctgcat gatgtattgt
     4261 gagaaatcga gcaaggtatc gaaaacctgt gtgtgttcgg gtaattcgtg tccgtgttgg
     4321 gagagcgttt ttttgccttt tccggtcaga accagcgcgg gttttccgcc gacggcatcg
     4381 attgcctgca aatcgcgcag gctgtcgccg accagccagg tttccgaggc ttgggcgttg
     4441 aagcgtccga tgatgtcttc aatcataccc ggtttgggct tgcggcagtt gcagttgtcg
     4501 gcatcggtgt gcgggcagaa ccagatgccg ttgatttcgc cgcctgcctg acggacgagg
     4561 cggtgcattt tggcgtgcat ttcggtaagg ttttgaacgg taaaatattt gcgcccgatg
     4621 ccggattggt tggtggcaac ggcgacggtg tagcctgcct gcgtcagaaa tgccaccgca
     4681 tccatgctgc cttcgacagg tatccactcg tcaacggatt tgacgaagtc atcgcggtcc
     4741 tgattgatga cgccgtcgcg gtcgagaatg atgagtttca tcgcgtttcc tttgggttgg
     4801 ggcgtgtcgg ggatgggatt ataggacata aatctagatt aaattctgct acaagatgtt
     4861 tctctaactc caatatttta tcttgtgatc tgcgcttcag tggtttacaa tctaccccga
     4921 cataaatagt ccacggctca agattttttc tcactatagc ccctgctcca acagcaaccc
     4981 catcaccaat agtaacgcct ggtaatactg ttacatttgt tcctaaaatt gcatgttttc
     5041 caattctaac actagatttt ttaacattag tatatttaga tgggactgtt ggattggtta
     5101 accccccccc ccccataaaa tcatcgctac cggtaataat tctacaccct gcgcttaatc
     5161 ctgaaaaatg atccatataa aattctccac caccaataat actggtaaat gatgatatat
     5221 gaacaaaact tcctaaaata cttttctctc cacaattaaa aaatacaaaa tcatcaattt
     5281 gagaataatc cccaattgat aaattttctt ttttaataat ctttgcacca tcgtatattt
     5341 tataattcac tttttagttc ctctaaaagg gctcattgta acttcaccat tttcagtgat
     5401 tccatctcga ataattatac tgaaatatcg gcagtgatgc gcctgtgctg cattataatg
     5461 cacgctttcg ataataattc gcaacggctc aggtatgccg ttttgaggag tggggcggga
     5521 tggttgccta tgttttcctg tttttgtttg taacggcggc gttggtgctg attatcaggt
     5581 cgcactaccg ctggacgtat tttttcgcat ccgcgctgtt tgtctttttg gcgggcggta
     5641 tgctgatgtt gacggctcag tggcaacgtg ccttgaattt cgcttcggtc tggtttgtgg
     5701 tgctgatact gttccacagg ctgaaaatcc attattacaa acagccgctg ttgatttccg
     5761 actttttgct gattgccgac tggcggaatt gggaaacgct gttccattat aaggaagcgg
     5821 ttatcggtat ggcggggctg ctggctttgg cggcatatgc ggttttcggc tggagcggtg
     5881 cggattcttt ggatgtgccg tggcggtggg caggcgcggt tttgtttgcg gcggcgttcg
     5941 tgtcgatgcg gcatttttcc aaacatccgg gcgcggtaaa gacgtggctg gattcgctgc
     6001 cggacgacgg gcgcgacgtg tttttgaacc tgccgatgtc ctgtcgggcg gtgtttttcc
     6061 aagtacccgt gttcgagggc gacggggaag cgtttgccag gcagatgccg tctgaaaccc
     6121 ggccgtgcgg catgtcggat gaaaaacccg atattgtcgt taccctgatg gaatcgacgc
     6181 tcgatccgca ctgtttcgac tttgccgccg ccaagattcc cgatttgaaa atgttcggac
     6241 ggcaggaaga taccgtattt tcctcacctt tgcgcgtgca tactttcggc ggcgcaacgt
     6301 ggaagtccga atttgcgttt ttggcgggtg ttccgtccac ggatttcggc gcgttggcaa
     6361 gcggcgtgtt ttactcggtc gtaccgcatt tgcagaccgg ttttgtccgc aacctgcgcg
     6421 aacacggtta tttttgcgtg gcactctcgc catttaccaa gggcaactac aatgccaagg
     6481 cagcatatga ccatttcggc tttaatctga tgttccagcc gcaagatttg ggctatcccg
     6541 cgccgatggg caaaaacctg tggcatattt ccagtgagga aatgatgcag tatgcgcgga
     6601 tgattttgga aaaacgccat cccgatttgg aaaacgtgcg gcagccgatg ttcgtatatg
     6661 tgctgaccat gaaggagcac gggccgtatc ggacggatac cgacaatgtg tttgatttgg
     6721 atgcgcccga tttgaatgcg aaaaccgtat ccgcgcttaa cgactacatc gggcgcattg
     6781 ccgatttgga caaagcggtg gaaagtttcg accgttatct gcacgaacgc ggcaaaccct
     6841 ttgttttcgg ttatttcggc gatcatcagg taccgtttga gggcgtgtcc gtcaggaaga
     6901 aatgggatta cgcgcagccg gattatgtaa cgcagtttgc cgtcaggagc aatattgccg
     6961 gcggattcgt acagcggcag aatttcctcg accttgcctt tgcaggcggc gtgctgatgg
     7021 aggcggctgg tctggaagcc aaagacggct tcatgcgtgc gaatatggcg atgcgcggtt
     7081 tgtgcggcgg agggttggaa gactgcccga actgggagtt ggtcggaaat taccgcaact
     7141 atctgtacga cgttttgaaa attgcccgtt agctttttgc cgcatggctg caacggttgc
     7201 gaaaatgccg tctgaaaact cgttcagacg gcattttgcc tttatccttt aaatacgggg
     7261 acgagttcca tttggctcaa tacctgtgcg gcgatgttga cgatgccgaa aaggaagacc
     7321 caaaccatca gccacaagcc gccgtaaact ttataggttt tgcctgcgcc gaattttttg
     7381 cgcgaacggt agagcagcat ggcggggatg atgcctgtcc agacggttgc cgccaggccg
     7441 acgtagccga tggcggtaac aaagccggtg gggaagagca ggcaggaaat tagaggcggc
     7501 aggaaggtca gcgcggcggt tttggtgcgg ccggacacgc tgtcgttcca tttgaagatg
     7561 tcggcgatgt agtcgaagag tccgagcgtt acgcctaaaa acgaggtggc gatcgccata
     7621 taggaaaaca gggacaatat tttgtccata ttgccggttt gggcgaattt ggacagggtt
     7681 tcaatcagga cggagacttg cccttcggcg gcaatcacgg gggcgaactc gttgcgcggc
     7741 aggttgcctt ggatggcggt ttgccagagg acgtaaatta ccagcgcaat cagtgtgccc
     7801 gtccagatgg atttagccac tttgggcgcg tcgcctttaa agtatttgag caggctggag
     7861 acgttgccgt ggaaaccgaa tgacgcaagg cagacgggca gggcggtggc gacataaatc
     7921 cagtagttgg tgccggtagg ggcttgggtg tcgaagagga cgggcagttt ggcatcggca
     7981 atcaggccgc cggttgccca aataaaggtt aataccatgc cgccgatgag gacgctggtg
     8041 aatcggtcga ccaagcgtgc ggatgcccat acgcaaaagg cgagaatgcc gaagaagacg
     8101 agttgtccga cggtgagtga aacattgccg cctgccgcgc tgcctaagcc tttggcggtc
     8161 aggtcgccgc cgacgaagat ataagcgtaa gtaagcaggt ataaaacgaa ggcgacggcg
     8221 atgccgttga tgatgttcca gctccgtccg agcaggtctt taaccatggt gtcgaagctc
     8281 gcgccgtggg ggtagtgggt gttgacttcc aaaatcatca ggccgctgga gagcatggaa
     8341 aaccaggtgt acagcaacac ggccagcgag ccggtaaacc atacgccgga tgtggcggtc
     8401 gggttggcga gcatacctgc gccgatgacc gtgccggcga taatcatcgc gccgccgaac
     8461 agtgaagggg ttttggtggg catattttgt ctttctgcca gaaaaagcga gccgccatta
     8521 tgccgtaaag tgtaaggatt tgtaaggtat ttgcgccgcg ccgcccgaaa aggctttcag
     8581 acggcattgc gttccatagt ataatcttgg gttttggagt gggcggttcg tcagatggga
     8641 gggaaaatgt ccgacaaaaa atataatgtc gatgaggggg aaatcgccaa attcagccgg
     8701 attgccgaca aatggtggga caagtcgggc gagttcaaaa ccttgcacga catcaatccg
     8761 ctgcggctgg attatatcga cggacacgcg gatttgtgcg gcaaacgggt tttggacgtg
     8821 ggctgcggcg gcggcatctt ggcggaaagt atggcacggc gcggcgcggc gtttgtaaag
     8881 ggtatcgaca tggcggagca gtcgctggaa accgcccgcc tgcacgcggc tttgaacaat
     8941 gtcgccgata tcgaatacga atgtatccgc gtggaagacc ttgccgaggc ggaaccgcac
     9001 tcgttcgatg tggtaacgtg catggaaatg atggaacacg tccccgatcc cgccgccatc
     9061 gtgcgtgctt gtgccaatct ggtcaaaccg gacggcatgg tgtttttttc caccatcaat
     9121 aaaaacccga aatcgtacct gcatctgatt gtggcggcgg aatatctgtt gaagtttgtc
     9181 cccaaaggca cgcacgactg gaaaaaattc atcgcgcctg ccgagctggc gcggatgtgc
     9241 cgtcaggcag gcttggatgt ggcggatacg aagggtatga cttaccatgt gttgtcgcaa
     9301 acttatgccc tgtgcgattc gaccgatgtg aattatatgt ttgcctgccg tccggcgttc
     9361 tgacggcggg tttgcccgtt tttgagcaag tgagttgata tgtctgtcta taccagtgtt
     9421 tccgatgatg aaatgcgcgg cttcctgagc ggttacgatt tgggtgaatt tgtttccctg
     9481 cagggcatcg cgcaggggat taccaacagc aattattttc tgacgacgac ttcgggacgt
     9541 tatgtgctga ccgtgtttga agtgttgaaa caggaagagc tgccgttttt tctggagctt
     9601 aaccggcatt tgagtatgaa gggcgtggcg gttgccgcgc cggttgcgcg caaagacggc
     9661 cggcttgatt ccgttttggc gggcaagccg gcttgtttgg ttgcctgcct gaaaggttcg
     9721 gataccgcgc tgccgacggc tgagcagtgt ttccataccg gcgcgatgtt ggcgaaaatg
     9781 caccttgccg ccgccgattt ccctttggaa atggaaaacc cgcgttacaa tgcgtggtgg
     9841 acggaggcgt gcgcccggct gctgcccgtc ctgtcgcaag acgatgccgc gctgctgtgt
     9901 tccgaaatcg atgcgttaaa ggacaatctc ggcaatcatc tgccttcggg catcatccat
     9961 gccgatttgt ttaaagacaa tgtgttgctt gacggcggtc aggtatcggg tttcatcgat
    10021 ttctattatg cctgccgggg caattttatg tatgacttgg cgattgcggt caacgattgg
    10081 gcaaggacgg cggacaataa gttggatgag gcgttgaaaa aggtgtttat cggcggttat
    10141 gagggcgtgc gccccttgag tgccgaagaa aaggcgtatt tcccgaccgc ccaacgtgcc
    10201 ggctgcatcc gtttttgggt gtcgcgcctg ttggactttc attttccgca ggcgggcgag
    10261 atgacgttta tcaaagaccc gaacgcgttc cgcaacctgc tgttgagttt ggattgagtg
    10321 cgtccggcgt ttgacagaaa tgccgtctga aagggtttca gacggcattt ttatggctga
    10381 ttaaaacgaa aatgagacga tagccgggta ttttc
//
LOCUS       DAWKOG010000057        10308 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.057, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000057 DAWKOG010000000
VERSION     DAWKOG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10308
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.057"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(142..939)
                     /locus_tag="WJK66_001084"
     CDS             complement(142..939)
                     /locus_tag="WJK66_001084"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEZ7578806.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVTGVSTVLARLPLTAP
                     QIESEPESEPALQTAPVETAANPSAEQRQIEAAAAKIQAAVQDVLKD"
     gene            1094..3943
                     /gene="uvrA"
                     /locus_tag="WJK66_001085"
     CDS             1094..3943
                     /gene="uvrA"
                     /locus_tag="WJK66_001085"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEZ7578807.1"
                     /translation="MYKHHPQHSHDNDTIRIRGARTHNLKNVDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHNLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPAVRERKGEFVDFFADLQAQGFARVCVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDSEKVVAHPELSLASGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPAKIKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVGGEPLHEVSAWPLTKTHR
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCEQSITGQYLSSKKSIAVPSERTPVNPDRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGIPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGRIIACGSPEQVAKVKGSYTGKYLKVVLR"
     gene            3977..5572
                     /locus_tag="WJK66_001086"
     CDS             3977..5572
                     /locus_tag="WJK66_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="funZ protein"
                     /protein_id="HEZ7578808.1"
                     /translation="MKPLKTLEFGFVDAANYRRRENKDLFNRIFVKGEYLDELCEPNI
                     SFLIGEKGTGKTAYAVYLTNNFYKNIHATTKFVRETDYSKFIQLKKARHLTVSDFTSI
                     WKVILYLLISNQIKCKENGILSSIFNKFKALDEAINEYYYGAFDPEIVQAITLIENSK
                     EAAEMIFGKFVKLGEEESQQITFTESKFQANLGFIERKFKDALSQLKLKDNHILFIDG
                     IDIRPSQIPFDEYHECVKGLANAIWMLNNDIFPSIKDSKGRMRVVLLIRPDIFDSLGL
                     QNQNTKLQDNSVFLDWRTDYKSYRSSKIFGVFDHLLRTQQEKQDSLEKGNSWDYYFPW
                     NAPNLHDEYKNLTSFISFLRKSYYRPRDILQMLTLLQKNKKSKEDYVVAEDFDNTSFQ
                     REYSIYLLGEIKDHLLFYYSQSDYQNFLKFFEFLNGKDRFKYSDFLKAFERLKKHLQT
                     TSVEIPKFMSTANEFLQFLFDLNVIAYLDNPEDETKPYIHWCFKDRNYANISPKIKTE
                     TEYLIFSGLSKALDVGTPFKNKQ"
     gene            complement(6123..7013)
                     /gene="sucD"
                     /locus_tag="WJK66_001087"
     CDS             complement(6123..7013)
                     /gene="sucD"
                     /locus_tag="WJK66_001087"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEZ7578809.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            complement(7024..8190)
                     /gene="sucC"
                     /locus_tag="WJK66_001088"
     CDS             complement(7024..8190)
                     /gene="sucC"
                     /locus_tag="WJK66_001088"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEZ7578810.1"
                     /translation="MNLHEYQAKELLASYGLPVQGGILAHNGEEAAAAYDKLGGKFAV
                     VKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTDANGQPVNSVLVCEDM
                     YPVQTELYLGAVVDRSTRRVTFMASTEGGVEIEKVAAETPEKIFKVTVDPLVGLQPCQ
                     AREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLAVRENGALACVDGKIG
                     IDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIGCMVNGAGLAMATMDI
                     IKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVKGVLINIFGGIVRCDMIAEAIV
                     AAVKEINVDVPVVVRLEGNNAELGAKILNESGLKLTSADGLNDAAEKIVAAVNA"
     gene            complement(8289..8576)
                     /locus_tag="WJK66_001089"
     CDS             complement(8289..8576)
                     /locus_tag="WJK66_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578811.1"
                     /translation="MNKEIVGIFFIPAGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            complement(8671..10104)
                     /gene="lpdA"
                     /locus_tag="WJK66_001090"
     CDS             complement(8671..10104)
                     /gene="lpdA"
                     /locus_tag="WJK66_001090"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ7578812.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHEFAEHGITVGDVKFDVAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNSDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
BASE COUNT         2674 a         2635 c         2388 g         2611 t
ORIGIN      
        1 tgataaatca ccgtaacgtt aaatttccag attcccattt tcgtgaacat aacggtgtga
       61 acattagcaa aaattcaaaa aaccaaactt cctcattcgt gtgaatgatg gtactgatat
      121 tttctatttc agttcgcaaa attaatcttt caacacatct tgcacagccg cctgaatctt
      181 agccgccgct gcctcgattt gccgttgttc ggcagatggg tttgccgctg tttcaaccgg
      241 agcagtttgt aaagcaggct cagactcagg ctcggattcg atttgcggcg cagtcagcgg
      301 caaacgcgcc aatacagtgc taacgccagt tactttatcg ccaatcgcca cttgcgcctg
      361 cgcatcgaca ggcagataca tatccacgcg cgaaccgaag cggataaagc cataacgttc
      421 gccgcgggac agtttcgcac ctgcttgggt gtagcacaaa atacggcgcg ccaccaaacc
      481 ggccacttga acaaaagtaa tttcacgacc ggaagccgta gtcgccaaca ccgcattacg
      541 ttcgttttcc gtgctggctt tgtccaaatc cgcattcacg aatttgcctt tgttatagac
      601 cactttcgtt accgtacaat cggcaggcga tttttgcgaa tgcacgttga acacgttcat
      661 aaaaatactg attttcaaag catcgacatc acgatacgga tcgcgtgcgc gctcgaccac
      721 cacgatacgg ccgtcaaccg gactcaacac tgcttcagga ttttgcggaa tctcacgcgc
      781 agggtcgcgg aaaaactgca gggcaaatac ggtaaacacc caaaacggca aagaccacca
      841 gccgcaacac atcgacacca gcaggctcaa agccaaaccg ccgccaataa tcggccagcc
      901 ctcacgggcg ataatcgggt gggggtaaag acggttcatt tgtcatcctt ttgttcaagc
      961 gggttaaaat tgccgccatt ataacggaat ccgccgcgtt ccgccgacaa atgccgtctg
     1021 aacgcggctt cagacggcat ccgagaaagt gctacaatac cagttttcct ttatttttca
     1081 ggcggcatcc attatgtaca agcatcatcc ccaacattcg cacgacaacg acaccatccg
     1141 catccgcggc gcgcgcacgc ataatttgaa aaacgtcgat ttggacattc cgcgccacaa
     1201 gctcgtagtg gtaacaggct tatcgggcag cggcaaatcg tcgctggcat ttgatacttt
     1261 atatgccgaa ggtcaacgcc gttatgtcga gagcctttcc gcctatgcgc ggcagttttt
     1321 gcagatgatg gacaaacccg atgtcgattt gattgaaggt ctgtcgcctg cgatttctat
     1381 cgagcaaaaa tccaccagcc acaatccgcg ttccaccgtc ggcacggtta cggaaatcca
     1441 cgactacctg cgccttttgt acgcccgcgt cggcacgccg tattgccccg aacacaatct
     1501 gccgttgtcg agccaaaccg tatcgcagat ggtcgatgcc gtgttgaagc tgccggaaga
     1561 cacgcgcgtg atgattctgg cgccggcggt gcgcgagcgt aagggcgagt ttgtcgattt
     1621 ctttgccgac ttgcaggcgc agggttttgc gcgcgtgtgc gtggacggcg aggtctatca
     1681 gcttgacgaa gtgccgaagc tggaaaaaaa catcaagcac aacatcgacg tggtcatcga
     1741 ccgcgtgaaa gtgaaggcgg acatcaagca gcggctggcg gaaagttttg aaaccgcgct
     1801 gcgccacggc aacgagcgcg cgctggcgat ggagatggac agcggcgaag aacattggtt
     1861 ttccgcgcgt ttcgcctgcc ccgtatgttc gtacagcctg cccgaattgg agccgcgcct
     1921 cttttcgttc aacaacccga tgggctcctg cccgacttgc gacggcttgg gtaacaccaa
     1981 tttcttcgac tccgaaaaag tggtcgcgca tcccgaattg tcgctggctt cgggcgcgat
     2041 tgacggctgg gacaagcgca accagttcta tttccaaatg attcagtcgc tggcacggca
     2101 ttacggtttc gacgtgcagg ctgcttggga aaccctacct gcaaaaatca aaaaagtcgt
     2161 gttgcacggc tcgggcaaag aagtcattga tttcacttac ctgtccgaac gcggcaccac
     2221 cttcaaccgc agccacgcct tcgaaggcat catccccaat ctcgaacgcc gctaccgcga
     2281 aaccgacagc gaaaccgtgc gcgaaaaact gcgcgaatac caaaaccacc gcgcttgccc
     2341 gagctgcggc ggcgcacgtt tgcgcaaaga agcccgctac gtttacgtcg gcggcgagcc
     2401 gttgcacgaa gtttccgcct ggccgctgac caaaacccac cgattctttg aaacgctgga
     2461 tttggacggc aacaaaaaac aaatcgccga aaaaatcctc aaagaaatca ccgaacgtct
     2521 cggctttttg attaatgtcg ggctggatta cctgaatctc tcccgttccg ccgaaaccct
     2581 ttccggcggc gaagcccagc gcatccgcct cgccagccaa atcggcagcg gcctgaccgg
     2641 cgtgatgtac gttttggacg aaccctccat cggcctgcac cagcgcgaca acgaccgcct
     2701 gcttgccacc ctcaaacgcc tgcgcgattt gggcaacagc gtcatcgtgg tcgaacacga
     2761 cgaagacgcc atccgcgaag ccgatttcgt cgtcgatatg ggcccgggcg caggcgaaca
     2821 cggcggcaac gtactgattg ccgacacgcc cgaaaacgtc gccaaatgcg agcaatccat
     2881 taccggacaa tacctcagca gtaaaaaatc cattgccgtg ccgtctgaac gcacgcccgt
     2941 caatcccgac cggatgctcg tcctcaaagg tgcgcgcggc aacaacctca aaaacgttac
     3001 cctcgaactg ccgctcggtt tgattacctg catcaccggc gtatccggca gcggcaaatc
     3061 caccctgatt aacgacaccc tcgccaaaat caccgcccgc gaactcaacc gcgcccaaga
     3121 agaacccgcc ccatacgacg acatccgcgg tctcgaacat ctcgacaaag tcatcaacgt
     3181 cgaccaatcc cccatcggcc gcaccccgcg ctccaacccc gccacctaca ccggcctgtt
     3241 cacccccatc cgcgaactct tcgccggcat ccccctctcg cgcgaacgcg gctacaacgt
     3301 cggccgcttt tccttcaacg tcaaaggcgg ccgctgcgaa gcctgccaag gcgacggcgt
     3361 gattaaagtc gaaatgcact tcctgcccga cgtgtacgtc ccctgcgaag tctgccacgg
     3421 caaacgctac aaccgcgaaa cgctcgaaat ccaatacaaa ggcaaaaaca tcagccaagt
     3481 cctcgacatg accgtcgaag aagcccgcga atttttcgac gccgtcccca ccgtatcgcg
     3541 caaactgcaa accctgatgg acgtaggtct cggctacatc cgcctcggcc aatccgccac
     3601 caccctctcc ggcggcgaag cccagcgcgt caaactcgcc ttggaactct ccaaacgcga
     3661 caccggcaga acactctaca tcctcgacga acccaccacc ggcctacact tcgccgacat
     3721 cgccctgctg ctggaagtca taggtcgtct gaaaggcaaa ggcaactcga ttgtgattat
     3781 cgagcataat ctagacgtga ttaaaaccgc cgattggatt gtggacttag ggccggaagg
     3841 cggcgatggc gggggcagga ttattgcttg tggtagccct gagcaggtgg cgaaagttaa
     3901 ggggagttat actgggaagt atttgaaggt ggttttgagg taagtttaat tcaaaattaa
     3961 ataggagatt attaagatga aaccgttaaa aacattagaa tttggatttg tggatgctgc
     4021 aaactacaga agaagagaaa ataaagattt atttaaccga atatttgtaa aaggagaata
     4081 tttggatgaa ttatgtgaac caaatatttc gtttttaatc ggagaaaagg gaactggaaa
     4141 gacagcatat gctgtttatt taactaataa cttctataaa aacatacatg ccactactaa
     4201 gtttgttcgt gaaaccgatt attcaaaatt tattcagcta aagaaagcaa gacacttaac
     4261 tgtttcagat tttacaagta tttggaaagt cattttatat ctgttgatat caaatcaaat
     4321 caaatgtaaa gaaaacggaa tattatcttc aatatttaat aaatttaaag ccttagatga
     4381 ggctataaat gaatattatt atggcgcttt tgatccggaa attgtacaag caataacttt
     4441 aatagaaaat tcaaaagaag ctgcggaaat gatttttgga aaatttgtta aactaggtga
     4501 agaggaatcc caacaaataa cttttacaga aagtaaattc caagcaaatt taggttttat
     4561 tgaaagaaaa tttaaagatg ctttatctca gttaaagcta aaagataatc atattttgtt
     4621 tattgatggg atagatatta gaccatcaca gattccattt gatgaatatc atgagtgtgt
     4681 aaaaggtctt gctaacgcca tatggatgtt aaataatgat atcttccctt ccattaaaga
     4741 tagtaaggga aggatgagag ttgtgttatt gattagacct gatatctttg attcattagg
     4801 tttacaaaat caaaatacca aacttcaaga taattcagta tttttagact ggaggacgga
     4861 ttataaatct tatagaagtt caaagatttt tggcgttttt gatcatcttt tgagaaccca
     4921 gcaagaaaaa caagatagtt tagaaaaagg caactcatgg gattattatt ttccatggaa
     4981 tgctcctaat ttacatgatg agtataaaaa tttaacttca tttattagct tcctaagaaa
     5041 atcgtattat cgacctcgcg atattcttca gatgcttact ttgctacaaa aaaataagaa
     5101 aagtaaggaa gattatgtcg tagcagaaga ttttgataat acttcttttc aaagagaata
     5161 ctcgatatat ttacttggtg aaatcaaaga tcatcttttg ttttattata gtcaaagtga
     5221 ttatcaaaat ttcctgaaat tttttgaatt tttaaacggg aaagatagat ttaaatatag
     5281 tgatttttta aaagcatttg aacgtttgaa aaagcactta caaacaacat cagtggaaat
     5341 acctaaattt atgagtactg ctaatgagtt tttgcaattt ttatttgact tgaatgttat
     5401 tgcttattta gataacccag aagatgaaac gaaaccatat atccattggt gctttaaaga
     5461 tagaaattat gcaaatattt ctcctaaaat aaaaactgaa actgaatatt taatattttc
     5521 aggattatca aaagcccttg atgttggtac tccatttaag aacaaacagt aacttgtatc
     5581 ccaaccaacc gtgtgcgcta cgcacacggt tggtagctga cgtgttgcag tttcaacaga
     5641 gaaagccgta gcctgcatac ccaaccgacc gcgtgcgtgg ctgtgccaca cgccctacct
     5701 gtgaattaca cggaacttca aagccaatac gagagctttg caaaattcct ttccctcccg
     5761 acagccgaaa cccaaacaca ggttttcggc tgttttcgtt tcaaatatcc actaatttta
     5821 ccccaatacc cccttaatcc tcccaggata cccgataatc aggcatccgg gccgcctttt
     5881 aggcgcaaac aggcacactt agcccgagac ctttgcaaaa ttccccaaaa tcccctaaat
     5941 gtcttggtgg gaatttaggg gattttgggg aattttgcaa aggtctctga ctgccccaaa
     6001 agggaaaaaa taatgtgggt atgttgaggc tgcctctttt tgaaatgaca aacaaaatgc
     6061 cgtctgaaac ccgatttccg gtttcagacg gcatttgatg tttaatcagt tgtcaaacct
     6121 gattatgcca aaccttttgc cttcaacact tccagcatcg tcgtgcccaa ctcggcaggg
     6181 ctgcgagtat aggcgatacc ggctttttcg aaagcggcga atttttcttc ggcagtacct
     6241 ttaccgccgg aaataatcgc accggcgtgg cccatacgtt tgcctttagg agcggtaaca
     6301 ccagcaatgt aacctacaac aggtttggtt acgttggatt ggatgtattc ggctgcttct
     6361 tcttccgcag taccgccgat ttcaccaatc atgatgatgg cgtcggtatc tgggtcttct
     6421 tggaagagtt tcagtgcgtc gatttggttc ataccaggaa tcgggtcgcc gccgataccg
     6481 atacaggttg attgacccaa gcccagtttg gtggtttgtg ccacggcttc gtaagtcaat
     6541 gtaccggaac gggaaataat accgatgcgg cccggagtat ggatgtggcc cggcataatg
     6601 ccgattttgc actcgcccgg agtaatcacg cccgggcagt tagggccgac caagcgggta
     6661 ccgttgccgt tggtttccaa gtaacgtttg gctttgagca tgtccaaagt aggtacgcct
     6721 tcggtaatca caacgaccaa gcctacgcct gaatcaacgg cttcaacgat agaatccaac
     6781 acaaacggag cgggaacgta aattacggat gcatccgcgc cggtttcttt aacagcttct
     6841 ttcatggtgt tgaatacagg caggttcagg tgggtttgac cgcctttgcc cggggtaacg
     6901 ccgccgacaa ctttagtgcc gtaagccaaa gcttgttcgg agtggaaagt accgttttta
     6961 ccggtgaaac cttgaaccaa tactttggtg tctttattaa tcaatacgct cattcttttc
     7021 tccttaggcg tttacggctg caacaatttt ttcggctgcg tcattcaggc cgtctgcaga
     7081 agtcagtttc aggcctgatt cgttcaggat tttcgcgccg agttcggcgt tgttaccttc
     7141 caaacgaaca acgacaggaa cgtcgacgtt gatttcttta acggctgcca cgatggcttc
     7201 cgcaatcatg tcgcaacgta cgataccgcc gaagatgttg atcaatacgc ctttaacgga
     7261 tttgtcttcc aaaatcagtt tgaacgcttc aaccacgcgg tctttggttg cgccgccgcc
     7321 aacgtccaag aagttggcag gttgaccgcc tttgagtttg atgatgtcca tggtggccat
     7381 cgccaaaccg gcaccgttaa ccatacagcc gatgttgcct tccagggcaa catagttcag
     7441 gtcgaattca gaagctttca gttcacgttc gttttcttga gatttgtcgc gcaattcagc
     7501 gatttttggc aggcggtaga gcgcgttgct gtcgatgccg attttgccgt ccacgcaagc
     7561 cagtgcgccg ttttcgcgaa ctgccagcgg gttgacttca aacagggcga agtcgttttc
     7621 gacaaacgct ttgtacgcgc cggtcatcag tttgacgaac tcgttgattt gtttgtcttt
     7681 caagcccagt tggaaggcaa cttcgcgagc ttggcaaggt tgcaggccga ccagcggatc
     7741 aacggttact ttgaagattt tttctggagt ttcggcagca actttttcga tttctacgcc
     7801 accttcggta gaagccatga atgtaacgcg gcgggtagaa cggtcaacca ctgcgcccaa
     7861 gtacagctcg gtttgaaccg gatacatgtc ttcgcaaacc aaaacgctgt tgacaggttg
     7921 gccgttggca tcagtttggt aagttaccaa gttggtgcca atcaggcttt cagccacttc
     7981 tttagcttct tcgcggcttt taacgacttt tacgccgccc gctttaccgc ggccgccggc
     8041 gtgtacttgt gctttgacaa cagcgaattt gccgcccaat ttgtcgtaag ctgcagcggc
     8101 ttcttcgccg ttgtgtgcca aaataccgcc ttgtacgggc aaaccgtagc tagccagcag
     8161 ttctttagcc tgatactcgt gtaaattcat ggatttctcc tttagattag tggaatgccc
     8221 cgagtttgcc tccaaatggc aagcccgtgc gtttcagact acctttcggc ggtcggaaaa
     8281 tcgtcgtttc acttgctgaa aggcaaccac atccgcgcca gcagatacat ggctgcaccg
     8341 cctcccccga gaataaaaaa gacgatgcct tttttacgcg aattcggaca gagcagataa
     8401 acggcaaggc tgaaactgat aaacaacaat gccacgcgcc aaaccaattc tttatctttg
     8461 aaacggttga gcgtataagt ttcggtcatc atcacataca tctgccacaa tgcggccata
     8521 cacatgctga tgatgcccgc cggtataaag aaaataccga ctatttcttt gttcataatg
     8581 aatgtaaatg ctgtttgttt gtctgacgga attataaatc agaaaacgaa gctctcggaa
     8641 cattcaattt gtcggaggcc ttaatgtcta tcaaccgtgc aaagcgcgtt tgtcggccgc
     8701 caatgcagct tcgtgaacca cttcggacaa ggttgggtgg gcatggataa tgcgggcgat
     8761 gtcttcgctg ctggcgaaga attcgagcgc agtcacgcct tcggtaacca attcgctgac
     8821 aaccggacca atcatgtgta cgcccaagat gcggtcggtt ttggcgtctg ccaacacttt
     8881 aaccgtacct ttggctttgc ccattgccaa tgcgcgacca ttcgcaccaa aacctgaagt
     8941 accttttttg tactccacgc cttcggcttt aagctgttct tcggttttac ccacccaagc
     9001 gatttcagga tcggtgtaaa taacaaacgg tacgttgttg aaatcgatat gcggtttttg
     9061 accggcaatg cgctcggcaa cggcaacgcc ttcgtcgctg gctttgtgtg ccaacatcgg
     9121 gccgcgaacc acgtcgccga ttgcccatac attaggcagg ttggtgcggc attcgccatc
     9181 tactttgata aagccgcgct catctttttc caagccgacg gcttcagcgt tcaggccttt
     9241 ggtgtttgga atacggccga tggcaacgat cagtttgtcg aatacttcgg ttttggcttc
     9301 gccggcagca gtttcgtaag caacggaaac acctttgcct tcagacttga tgtcgccgat
     9361 tttcacgccc aattcgatgc tcagaccttg ctctttggtg aagtatttga aggcttcttt
     9421 ggcgatttgt tggtcggcgg cagccaggaa ggtcggcgcg gcttcaagaa tggtaacttc
     9481 cgcacccacg cggttccata cggaacccat ttccaaacca atcacaccgg aaccgatcac
     9541 gccgagtttg gcaggtactt cggtcaggtt caatgcgcct tcgttgtcca atacgttcac
     9601 attgtcgata gcgacttgtg gcagcggacg cggtacggaa ccggtggcta cgatgacgtg
     9661 tttggcttcg ataacggttt tctcaccttt gttatcgact tcgatttggt aagcatcgct
     9721 atttttgccg gcaaaggaag ccgtaccgaa caggctggtt actttgtttt tctggaacag
     9781 gaatttcacg ccgccggtca gtttggttac gatggcatct ttgcgctcaa tcattttggc
     9841 cacgtcgaat tttacgtcgc cgacagtgat accgtgttcg gcaaactcgt gttgcgctgc
     9901 gtggaaatgt tcgctggatt gcaacagggc tttagaaggg atacagccta cgttcaagca
     9961 agtaccgccc aatgcagggg cattgcctgc tttgttaacg cctgcatcga cacaggcagt
    10021 tttgaaaccc agttgtgcgg cacggatggc ggcaacgtat ccgcccgggc ctgcaccaat
    10081 cactactaca tcatattgag acatcgtgtc atcctttgct tttagcagta tctcaaaata
    10141 aaaacgatac ttttcgtttc ttttttattt tgaatgactg cgttttgatg gttttaagaa
    10201 aaagccgtct gaaagtccat atctgccgat tcattcgaat caaatgtttc agacggcctc
    10261 aatcgttcgt tgagcctatt gtagcacgat actacattac gctacatc
//
LOCUS       DAWKOG010000058        10094 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.058, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000058 DAWKOG010000000
VERSION     DAWKOG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10094
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.058"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..200
                     /locus_tag="WJK66_001091"
     CDS             <1..200
                     /locus_tag="WJK66_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ7578813.1"
                     /translation="DGIMRKACRNRPLSEAQTKRNRYLSKTRYVVEQSFGTLHRKFRY
                     ARAAYFGLICARCRLKGSPDA"
     gene            complement(456..668)
                     /locus_tag="WJK66_001092"
     CDS             complement(456..668)
                     /locus_tag="WJK66_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7578814.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAKPD
                     TETRRTDMSLEKWRQFLDWTEKTNAV"
     gene            complement(680..961)
                     /locus_tag="WJK66_001093"
     CDS             complement(680..961)
                     /locus_tag="WJK66_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578815.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDSEC
                     PGGHYRHSENGITLYDFIFFDGKPADEARFAAVLTETCRRAVKKICETK"
     gene            complement(999..1262)
                     /locus_tag="WJK66_001094"
     CDS             complement(999..1262)
                     /locus_tag="WJK66_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578816.1"
                     /translation="MADILPSDKRRQPPKTKGSTMNIIEQEMKHSSLRKVDAEIAKII
                     ADAHKINAESVKIAQESRWYPMMAATGLVTAIAAVLALIFKFA"
     gene            complement(1282..1854)
                     /locus_tag="WJK66_001095"
     CDS             complement(1282..1854)
                     /locus_tag="WJK66_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEZ7578817.1"
                     /translation="MGKPGKSSRPSALLSARWQDIDLTARLWHIPAADMKTKHPFTVP
                     LSDWAVEILRELHTQTGDNIHLFPGIRPRRKPAPHPDHISIKFAHNAIRRLGYDGSTP
                     GKSKHTMHGFRHLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHHYLTADSYR
                     LQERRELAEWYSRRHRQAYEAAAQNTCNKT"
     gene            1833..2144
                     /locus_tag="WJK66_001096"
     CDS             1833..2144
                     /locus_tag="WJK66_001096"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578818.1"
                     /translation="MTSPVSPSIRAASIFPLARSISTACRTDINRLPLRLIASTKTAE
                     INLASSGLSSVLPASLRRLRVGLTLTASSLRYSVARLMPNSCACCLRYAERAPRSIAS
                     A"
     gene            complement(2166..3041)
                     /locus_tag="WJK66_001097"
     CDS             complement(2166..3041)
                     /locus_tag="WJK66_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEZ7578819.1"
                     /translation="MNTEIEIIATDKVSNQAAMRSVLVMEQWGNGETYSEERWVERGR
                     QAVRQTMEGMFELGRALIILKEHTEHGRFMEIVKSQFGLGIAETSRLMSATRRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELAEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHGISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(3086..3376)
                     /locus_tag="WJK66_001098"
     CDS             complement(3086..3376)
                     /locus_tag="WJK66_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ7578820.1"
                     /translation="MAESKRVQRLLRVFIALDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRQQHERLQSKIAEIGKRVDVD"
     gene            complement(3388..3567)
                     /locus_tag="WJK66_001099"
     CDS             complement(3388..3567)
                     /locus_tag="WJK66_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ7578821.1"
                     /translation="MYGYTVEKLKENFAQNGQTLAQWARENGFKPRDVYLVVGGQRKG
                     NYGKGHEIAKKLGLK"
     gene            3681..4058
                     /locus_tag="WJK66_001100"
     CDS             3681..4058
                     /locus_tag="WJK66_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7578822.1"
                     /translation="MISKSLFGNRLKEKRKFLGLTQAQAAEKAGIERETWGKYERGVF
                     MPSGDVLLSFLNMGIDVSSLFAAEQGIRPSENSEISKEESELLGYYRQASDNGKFVIL
                     SVSKGAEKKAAENTQVSATHKTA"
     gene            4087..4332
                     /locus_tag="WJK66_001101"
     CDS             4087..4332
                     /locus_tag="WJK66_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578823.1"
                     /translation="MFDFEQQIKWGERAEEIVKEAATQNNIEIPEPLASALAKAVKVH
                     YLSQAGVFSLVEAYADTVNPTEKEVDYQAIGKELFEK"
     gene            4566..5534
                     /locus_tag="WJK66_001102"
     CDS             4566..5534
                     /locus_tag="WJK66_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578824.1"
                     /translation="MAKKTTKTIHYLRAQQNHSLFDLEVALRRVLSAAPTVQDTQIEQ
                     YNQLTQIMHRTLDPKDVKGNSIGGLLIHIGSGTKDEHIRTMSNRPVQQDDHGGTQAPP
                     SGYSFLRKEAFLYIVGHHVIFCGHGFLSASTVASYLSLLSNKLRENNPDIVQFNIEFK
                     AVGNCDKLSLIQQHGVKSILLDASAYQLSRNRLYQDSRSTIAKALGKVGSVFTSELSD
                     EELEAQSEIHINLEVLLNGNSRASIEAQSLMQEQAEEIIDDETVNQGFSITTQQGEVI
                     QPSDVKLSKSVRIGRYDEANSLLPDSAFTAISEYFLELQSRNLTEQ"
     gene            5531..6013
                     /locus_tag="WJK66_001103"
     CDS             5531..6013
                     /locus_tag="WJK66_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578825.1"
                     /translation="MKKYLPLLRFLLIACISAFLAWKGQPFVHGNEKAVDLIINVFAI
                     LAGFLIAIMTLFSDMRFDEDANWRQIQIREGVQEQRYIKHSLLFYTYLAVLVCVFIVI
                     LLAHKEEYKNGPAIFWLERSYLFLACISIFYSVFLPGNLIKSRKEEFKKLMEKKKPKI
                     "
     gene            6103..6606
                     /locus_tag="WJK66_001104"
     CDS             6103..6606
                     /locus_tag="WJK66_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HEZ7578826.1"
                     /translation="MSDEFNRFIERVLSHEGGYVNHPKDPGGETNWGITKRTAQANGY
                     NGSMRAMTREQAIGIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAARMLQRAAG
                     VPDDGIIGEISLKAINSLPENDLLLRFNAERLVFYTKLGTFTSFGKGWVRRVAQNLIH
                     AAADNAD"
     gene            6715..7047
                     /locus_tag="WJK66_001105"
     CDS             6715..7047
                     /locus_tag="WJK66_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578827.1"
                     /translation="MLDASPPQERGSRRETRSQKKASQITAFFRWLGGLVSNPATGKI
                     SHTKLWANVAAAAMTWKFVQAADAPEWLWWAYGAMVGGYALIKRGIAVVPQLAEVKKS
                     ANQEGGRQ"
     gene            7044..7454
                     /locus_tag="WJK66_001106"
     CDS             7044..7454
                     /locus_tag="WJK66_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEZ7578828.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAEQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLKAEMVALKKPDALIHGKEVRQKERNSSQG"
     gene            7469..8191
                     /locus_tag="WJK66_001107"
     CDS             7469..8191
                     /locus_tag="WJK66_001107"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ7578829.1"
                     /translation="MNCCWGLSFYYQLAVQSAEDKYADLKRHIHDIYRRHKGRYGYRR
                     IAAAIRHAGTPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKIAPNILRRCFHAEK
                     PNEKWVTDVTEFNVGGEKIYLSPIMDLFNGEIVSYRIQIRPTFDLAGEILKGAPEKPR
                     SSEKPILHSDQGWQYQMFFYSGLNLNQDKAMKPQTVQIVRQGEATPYWFKFNPLYNND
                     RIKLKLKGLSLVQYRIQSLKAA"
     gene            complement(8512..8874)
                     /gene="ssrA"
                     /locus_tag="WJK66_001108"
     tmRNA           complement(8512..8874)
                     /gene="ssrA"
                     /locus_tag="WJK66_001108"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(8937..9542)
                     /locus_tag="WJK66_001109"
     CDS             complement(8937..9542)
                     /locus_tag="WJK66_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEZ7578830.1"
                     /translation="MNTDFQNILKQLGKQAQKEAAEKQAAEKNKQKQEQDFDFSQAVG
                     QVSPLKNRQQYYAPPDKTPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
BASE COUNT         2450 a         2327 c         2566 g         2751 t
ORIGIN      
        1 tagacggcat tatgcgcaaa gcctgccgca accgtccgct gtcggaagcg caaaccaaac
       61 gcaaccgata tttgtcgaag acccgttatg tggtcgaaca aagcttcggt acgctgcacc
      121 gtaaattccg ctatgcgcgg gcagcctatt tcggactgat ttgcgcccgc tgccgcctaa
      181 aaggcagccc ggatgcctga ttatcgggta tccggggagg attaaggggg tatttgggta
      241 aaattaggag gtatttgggg cgaaaacagc tgaaaacctg tgtttgggtt tcggctgtcg
      301 ggagggaaag gaattttgca aaggtctcag agtgagttta ttttggggcg gcggcaggtc
      361 gggggcaagc ggcgtggggc ttggttgtgg tttttaggtt tgggggtggg taaaaaatgc
      421 cgtacccggc gggcaggctt cgcactctga aggtttcaga cggcgtttgt tttttctgtc
      481 caatcgagga actgccgcca tttttccagc gacatatcgg tccgccgcgt ttcggtatcg
      541 ggtttggctt cccagcggcc tacctgtatg tagtgcttca ccccgacgat ttgcgccaac
      601 tcggcctgtg tcagtttgca gcggttgcgc agggtgcgaa ggttgtaagg cgtgtagccg
      661 agttccatgt cgttgcggtt tattttgttt cgcatatttt tttgactgcc cggcggcagg
      721 tttcggtaag gacggcggca aatcgggctt cgtctgccgg ttttccgtcg aaaaaaataa
      781 aatcgtaaag ggtgatgccg ttttcggaat ggcggtaatg cccgcccggg cattcgctgt
      841 ctgtctgcct gtggattttg gcaatcaggc ggggatagcg gcaatgggta atgaaacggc
      901 ttgcgccgtc ttgcccgata atccattcgg ggtagcggtt gaatagtgcg gctctgttca
      961 ttttgttcgg gggataaagc ccctcgcggg gcttgtggtc aggcaaattt gaatatcagt
     1021 gccaacacgg cggcgatggc ggtaaccagc ccggtggccg ccatcatggg ataccatcgg
     1081 gattcttggg ctatttttac ggattcggcg ttgattttgt gcgcgtctgc aatgattttg
     1141 gcgatttcgg catctacttt tctcagactg gagtgtttca tttcttgttc gattatgttc
     1201 atcgtacttc ctttcgtttt tggcggttgc cgccgcttgt cggatggtag gatgtctgcc
     1261 atgtgtatat attgatacct tttaggtttt attgcaagtg ttttgggcgg cggcttcgta
     1321 tgcttggcgg tggcggcggc tgtaccattc ggcgagttcg cgccgctctt ggaggcggta
     1381 gctgtcggcg gtcaggtagt ggtgcaggct tgagaagccg gcgcgttgga gggcggcgcg
     1441 gctgatgtgg ccgagggcga ggtctgttgt gagggtgtct tttccggcgg tcaggctgat
     1501 gttggtgaag aggtggcgga atccgtgcat tgtgtgtttg gatttgccgg gggtgctgcc
     1561 gtcatagccc agtcgtcgga tggcgttgtg ggcgaatttg atgctgatgt ggtcgggatg
     1621 gggggcgggt ttgcgccgtg ggcggatgcc ggggaaaagg tggatgttgt cgccggtctg
     1681 tgtgtgcagc tctcggagta tttctaccgc ccagtccgac agtgggacgg taaaggggtg
     1741 tttggtcttc atgtcggcgg cggggatgtg ccataaccgg gcggtgaggt cgatgtcttg
     1801 ccagcgggcg gaaagcagtg cggacggacg ggatgacttc cccggtttcc ccatcaatcc
     1861 gcgccgcctc gatttttccg ttggcgcgca gcatttccac cgcctgccgc accgacatca
     1921 accgcttgcc gttgcgcctc atcgcctcca ccaaaaccgc cgaaatcaat ttggcttctt
     1981 ccggtttaag ctccgtcttg cccgcatcgc tgcgccgttt gcgcgtcggc ttgacgctga
     2041 ccgcctccag cttgcggtat agcgtggcaa ggctgatgcc caattcctgc gcctgctgct
     2101 taagatatgc agagcgtgcg ccgcgttcca ttgcttccgc ctgattctcg actgccttaa
     2161 gacgctcaat cattgccgga ttcatcgcct tctcccgttt caccgcccaa ccattccggc
     2221 acattgtctg tcggtgcttc agtcggcagg gcatagcttt cgcgcagttg ctcgcagtcc
     2281 aaaataattt gattgagcgt gccgaccatc tttgcctgat ggctgatccc gtgtgcctca
     2341 ctgtgcgcat taagttggtc gaacaaatct ttcagacggc tcacttgact gcggataccg
     2401 acctcaaggc ttgttaactg catcgccaac tcgctgccta cgtcttccgc cttcggctct
     2461 ctgacaacgg tttgcttctt agccagcttc tcggccagct catcaatttt ggctgttttg
     2521 gttttcatca cttcgtcttt ggcggcgagg ttttcgcggc tttcgcgcag ggcgacgcgc
     2581 agctcgcgca ccgtcattcg gtccacatcg tcaaaggtca tgccgttgac ttcttcccct
     2641 tcggccaaac ccaccagcgt aacgtcttct tcgaccagca gctcaagcag cttcgacttg
     2701 cccaaatcca tcagcttcgg cgcggctttc tgcatttgcg gggtcgcaaa gcggcgagtg
     2761 gctgacatca gacgtgatgt ttctgcgata cccagcccaa attggctttt cacaatttcc
     2821 ataaaccgtc cgtgttccgt atgctctttt aaaataatca gcgcacgtcc cagttcgaac
     2881 atgccttcca tcgtctgccg tactgcttga cgaccgcgtt caacccaacg ctcttcgcta
     2941 taagtctcgc cgttacccca ctgctccatc accagcacac tgcgcatcgc tgcctgattg
     3001 cttactttgt cggttgcgat aatttcaatt tctgtattca ttttttatct ccaaagtttc
     3061 cgacgtcgga aactttcaaa atccgttaat ccacatcgac ccgcttgccg atttcggcaa
     3121 tcttgctttg cagccgttca tgctgctgcc tgaaccgctc tgcgatttgc agggttttga
     3181 tgccgtaggc gtagttgccg ttttcaagtt tgatgaccaa tcccgaggca accaaatcat
     3241 caatatccct gctgacttgc gatggcgtca gccccagtcc gaccgataaa tccttattgc
     3301 tcagaccgat aatcggatgc tcgtcaagcg cgataaagac cctcaatagc cgttgtaccc
     3361 ttttactttc tgccatccgc atcctcctta tttcagtccc agcttcttgg caatttcatg
     3421 ccccttgccg taattgcctt tgcgctgtcc gccgaccacc agatacacat cgcgcggctt
     3481 aaagccgttc tctcttgccc actgcgccag cgtttggccg ttttgggcaa aattttcttt
     3541 tagtttttca acagtatagc catagatagc cctttcccat tgtgataaaa tagtgaacta
     3601 tttatgcact agaaattaat tagtgcatta ccaatgcgtg aattatgaga ttttaaaatc
     3661 tcttttgcaa ggattatttt gtgatttcta aatctctttt tggtaatcgt ttgaaagaaa
     3721 aaagaaagtt tttagggctt acccaagcac aagctgctga aaaagcaggt attgagcgcg
     3781 aaacatgggg caaatacgaa cgtggcgtat ttatgcctag tggagatgta ttgctctctt
     3841 ttttgaatat gggaatagat gtgagcagct tatttgctgc tgaacaggga attaggccgt
     3901 ctgaaaattc tgaaattagt aaggaagagt cagagttatt agggtattac cgccaagcca
     3961 gcgataacgg caaatttgtc attctcagtg tgtcaaaagg cgcagagaaa aaagcagccg
     4021 aaaatactca agtttcagcc acgcataaaa cagcgtgatt tttaaatcca aaatggagac
     4081 agtcttatgt ttgactttga acaacaaatc aaatggggtg agcgagcgga agaaatcgtt
     4141 aaagaggcag ccacccagaa caatatcgaa attcccgaac ctttggcttc ggcattagct
     4201 aaggcggtaa aagtgcatta tctaagccaa gctggtgtgt tttctttggt agaagcctat
     4261 gcagacacgg taaatccaac tgaaaaagaa gtagattacc aagcaatcgg caaagagcta
     4321 tttgaaaaat aaaaaatgcc gtctgaaatt ttcagacggc atttaggaca ctccgacagt
     4381 cgggctgctc cggtgttctg aaacggatat taagcaattg aagcggccgt gcattttaaa
     4441 acgcattaaa aactaccccg acatacaggt catcgggcag gtggttcagg tgttgaaaga
     4501 tttgaattga tgccgtctga atttattttt tcgacacaga tattaaaaga gaaaggactg
     4561 tacaaatggc aaaaaaaact actaaaacta ttcattattt acgtgcgcag caaaaccata
     4621 gcttgttcga tttagaggta gcattaagaa gggttttgtc agctgctcct actgttcaag
     4681 atacacaaat tgaacaatat aatcagctta cccaaatcat gcatagaact ttggatccta
     4741 aagatgtaaa aggcaattca attggtggat tactgataca cattggcagc ggtacaaaag
     4801 atgaacatat ccgcactatg agcaatagac ctgtacagca agatgatcat ggtggaacac
     4861 aagctcctcc aagtggatat tcctttttgc gtaaagaggc ttttttgtat attgttggac
     4921 accatgtaat tttttgcgga catggttttt tgtcagcatc aacggttgct tcttatttaa
     4981 gtttacttag caataaattg agagagaaca atccggatat tgttcaattt aatattgaat
     5041 tcaaagcagt aggcaattgc gacaagttgt cattgataca acaacatggg gtaaaaagta
     5101 ttcttttaga tgcttcggca tatcaattat ccagaaacag actgtatcaa gatagccgtt
     5161 caaccattgc taaagcatta gggaaagttg gtagtgtatt tacaagcgaa ttgagtgatg
     5221 aggaattgga agcacaatcc gaaattcata tcaatttaga agtcttgcta aacggtaatt
     5281 cgagagccag tattgaagcg caatcgctga tgcaggagca ggcagaggaa attattgatg
     5341 atgaaacggt taatcaagga ttttccatta ccacgcaaca aggtgaagtg attcagccct
     5401 ctgatgtaaa actgtctaaa tctgttagaa taggacgata cgatgaagcc aactcgttac
     5461 tccctgactc ggcatttaca gcaatcagtg aatatttcct agaactccaa agcagaaact
     5521 taactgaaca atgaaaaaat atctgccctt actacgtttt ttgttgattg cttgtatatc
     5581 agcattttta gcatggaaag ggcaaccttt tgttcatggg aacgagaagg ccgttgattt
     5641 aattattaac gtatttgcaa tcttagccgg ttttttaatt gccattatga cattgtttag
     5701 tgatatgagg tttgatgaag atgcaaattg gcgacaaatt caaattcgtg aaggcgtaca
     5761 agaacaacgg tatataaaac attccttact tttttacaca tatcttgccg tattagtttg
     5821 tgtttttatt gtcatcttgt tggctcataa agaagaatac aaaaatggac cagccatttt
     5881 ttggcttgaa cggagctatt tatttttagc ctgtatttcc attttttatt ctgtattctt
     5941 acctgggaat ttaattaaaa gccgaaaaga agagtttaaa aaattaatgg aaaagaagaa
     6001 acccaaaatc tagaacgagt tttaaccccc tttaaaatcc cattcagacg accttttcta
     6061 aaatccctgt attgattttt aattcaatac agggattttt ccatgtcaga cgaattcaac
     6121 cgatttatcg aacgcgtcct ctcccacgag ggcggttacg tcaatcatcc caaagacccc
     6181 ggcggcgaaa ccaattgggg catcactaag cgcaccgcac aggcaaacgg ctacaacggc
     6241 tccatgcgcg ccatgacgcg tgaacaggca atcggcattt accgtaaagc gttttgggag
     6301 cgttaccgcg ccgaccaaat gcccgaagcg gtcgcttttc aattttttga tgcctgcgtc
     6361 aaccacggtt acggcaatgc cgcccgtatg ctccaacgcg ccgcaggcgt gccggacgat
     6421 ggaatcatcg gcgaaatcag cctcaaagcc atcaattccc ttcccgaaaa cgacctttta
     6481 ttgcggttca acgccgagcg tctggtcttt tataccaagc tgggtacgtt cacctctttc
     6541 ggcaagggct gggtacgccg tgtggcgcaa aacctgattc acgccgccgc agacaatgcc
     6601 gattgaggag caaaccatgt caaaaaagtc cattctcgcc ctgatggccg cagccatgca
     6661 gcccgatttc agccacagcg acctaggcat tcgctacgcc atgccgactc agggatgttg
     6721 gacgcaagcc caccgcaaga gcggggtagc cgccgcgaaa cgcgcagcca aaaaaaagcg
     6781 tcacaaataa ccgccttttt ccgatggctg ggcggcttgg tctctaatcc ggccacaggg
     6841 aaaatcagcc ataccaaact ttgggcaaac gtcgccgcag ccgccatgac ttggaagttc
     6901 gtgcaggcgg cggacgcgcc cgaatggctt tggtgggcat acggcgcgat ggtcggcggg
     6961 tatgcattaa tcaaacgcgg catcgccgtc gtaccgcagt tggcggaagt caaaaaatcc
     7021 gcaaatcagg aaggggggcg gcaatgattg aatttgtccg agccaaaaaa cggctgcttt
     7081 gggcatttgt gcttttgctt gtgtggacgt gcggttaccg atacgccgcc gacaaggccg
     7141 aagcggaaca aaccgccctg attgccacct atcggcattc ttctatggtt gcggcggaac
     7201 aatatgcctt gcagcttaaa aaagcgcagg acgaaaggca gcggtggtac gacttttccc
     7261 aaaaacaagg aagaaagccc gtgaaaaaac agtatccgcc gcaaacgaaa aaagccggct
     7321 atctgaaaac caaggaagaa ctgcttgcgg aattggcttg ccttaaagcg gaaatggttg
     7381 ccctaaaaaa gcccgatgcc ttaatccatg ggaaagaagt gcggcagaaa gaacgcaact
     7441 cgtcgcaggg ttaaggcaat gccatccgtt gaactgctgt tggggattgt ccttctatta
     7501 ccaattggcc gtccaatcgg cagaagacaa atatgccgat ttgaaacggc atatccatga
     7561 tatttatcga cgacataagg gaagatacgg ctaccggagg attgcggcag ccatccgtca
     7621 cgcaggaaca ccggtcaatc acaagaaagt cagccgtctg atggcgaaga cggggctgaa
     7681 ggcagtgata cggcggcgca aataccgctc gttcaaagga gaagtcggca aaattgcgcc
     7741 gaatatcctg cgacgctgtt tccatgcaga aaagccgaat gagaaatggg taacggacgt
     7801 taccgagttc aatgtaggcg gagaaaagat atacctttct ccgattatgg atttgtttaa
     7861 cggggaaatc gtcagttacc gtattcaaat ccgcccgact ttcgatttgg ccggcgagat
     7921 actgaaaggt gcgccggaga aaccgagatc gtctgaaaag ccgatactgc attcggatca
     7981 aggttggcaa tatcagatgt ttttttatag tggattaaat ttaaatcagg acaaggcgat
     8041 gaagccgcag acagtacaaa tagtacggca aggcgaggca acgccgtact ggtttaaatt
     8101 taatccacta tacaacaacg atagaatcaa gttgaaatta aaagggctga gccttgttca
     8161 gtacagaatt cagtccctga aagccgcttg attaaactgt ccgacttttt ggggtcagtt
     8221 cggcttcggc atttttttat ccgtttgggg gtaacttgtt tggaaagctg caagcttata
     8281 aataaaggat tacatttaag ttttgggtaa cctttttaaa aaaatgcgtg atgacttttg
     8341 cattcttaag gcgttttttg gggtaattcg tgaaaagagt taccccaaaa gttaccccat
     8401 aaatggcgaa aactcaagca tacgccagca tattcaaggc aacaaaaaag ccttgaaact
     8461 gttgaagttc aaggcttttt tgtgtttcag gatactgctg aaaatagggt atggtggagg
     8521 cggggggaat cgaacccccg tccgaaagtc ctctacaaag cgttctacat acttagttgt
     8581 gtctatttga aaatcttatt tccatcatgc cgaccaacag gccttttgga aaccagttac
     8641 cttaaatctc atttcctgcc aagtaacccg gcaggaaacc agtcaatgta agatgacgtt
     8701 gcggtggctt tcgccacaca gcccattgac cgactgctgc aacggctagc cttaagcggc
     8761 taaagcgtaa gtttcgtcgt ttgcgactat ttgaattcag tgttttacgg gaatctgaga
     8821 ccccggtatg cccgcatctg cttcgcaacc cccgtcgaaa ccaaggtcgc ccccaaaaat
     8881 ggtttgcaaa ttatacggat attgtgcggt gctgccaagt ctgtcggaga aatttgtcag
     8941 tcttgctgcc ttaatttgcg tttgagcagg atgcggacgc agccgtcgtt gccttcccgg
     9001 ggttcggcgt aggcgagtac atcggggtgt tgcatcagcc agtttcgggt catatttttc
     9061 agaacgggtt tgtagccttt ggaacctaat ccgctgccgt ggatgatttc gccgcatacg
     9121 ccgcgttttt gggtgaatgc gatgaattcg ttgaggactt tttgggcttc ttcctgtgtg
     9181 tagccgtgca ggtcgacatc ggtaacgacg ggatagtatc cgtttttcag gcgttggatg
     9241 tcgttttttc cctgtccgtt tttgctgaag ctggcgggcg ggtcgttgta tgtgctgccg
     9301 atgtagaagt agttttcttc gtcggcgcgg ttgtctttgg gacggacttt gatgggggtt
     9361 ttgtcgggcg gcgcataata ttgctgccgg ttttttaatg gggagacttg tccgactgct
     9421 tgtgaaaaat cgaaatcctg ttcttgtttt tgcttgtttt tttcggctgc ctgtttttct
     9481 gccgcttctt tttgggcttg tttgcccagt tgtttgagga tgttttggaa gtcggtattc
     9541 atattttttc ctgttatttg tccgatggct gtttcgggcg gggttttaat ttgccggaat
     9601 gtttgccaat cgggggagga tgattttgtt gccgtcgtat gttttttgaa agtgtgattg
     9661 tatatcaaaa agaaatgcgg caaccgtcgg cagtgttgat tgccggaaat gcgggccggt
     9721 cgaaccgata tgcccgaacg cctgataaag ttttaaaaac ctgccttgcg aagcaggtta
     9781 atggttttgc caattttgaa ttgccggaaa cgcgaaacac ggaaatctga tgttttataa
     9841 atgttccgat acgaactgca aaatattggt tgagaccttt gcaaaattcc tttccctcac
     9901 gacaaccgaa accccaacac aggttttcgg ctgttttcgc cccaaatatc tcctaatttt
     9961 acccaaatac ccccttaatc ctccccggat accctataat caggcatccg ggctgccttt
    10021 taggcggcag cgggcgcact taacctgttg gcggctttca acaggttcaa acacatcgcc
    10081 ttcagatggc tttg
//
LOCUS       DAWKOG010000059        10031 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.059, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000059 DAWKOG010000000
VERSION     DAWKOG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10031
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.059"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(119..1336)
                     /locus_tag="WJK66_001110"
     CDS             complement(119..1336)
                     /locus_tag="WJK66_001110"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEZ7578831.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLSTGEQVTIGLLAMALKDIGVEAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLAAGKVVIVAGFQGISSEGDISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1538..2266)
                     /gene="rph"
                     /locus_tag="WJK66_001111"
     CDS             complement(1538..2266)
                     /gene="rph"
                     /locus_tag="WJK66_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEZ7578832.1"
                     /translation="MPDYIRISRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2348..2812)
                     /locus_tag="WJK66_001112"
     CDS             complement(2348..2812)
                     /locus_tag="WJK66_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEZ7578833.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3029..3805
                     /locus_tag="WJK66_001113"
     CDS             3029..3805
                     /locus_tag="WJK66_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEZ7578834.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERVAQINPGCQVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKANMRVPCVYSTENIVPPQSREAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIADKK"
     gene            4050..4904
                     /locus_tag="WJK66_001114"
     CDS             4050..4904
                     /locus_tag="WJK66_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEZ7578835.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIEAYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTETVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHGIC
                     RFHDLFNPEQGAAYVVVNFIALLELAKEGLVGIVQEVGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            4945..6153
                     /gene="pncB"
                     /locus_tag="WJK66_001115"
     CDS             4945..6153
                     /gene="pncB"
                     /locus_tag="WJK66_001115"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEZ7578836.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIKEDLEAELDALCRLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEIGTD
                     DKDRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDDPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            6245..7963
                     /gene="argS"
                     /locus_tag="WJK66_001116"
     CDS             6245..7963
                     /gene="argS"
                     /locus_tag="WJK66_001116"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEZ7578837.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIADSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRPEFLAQNIQTALNDA
                     RFGVAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLEFMGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQAVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRVGTLHADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDADAPTVLTEPLEKQLAAELLKFENVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAEGASRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
     gene            8517..9935
                     /locus_tag="WJK66_001117"
     CDS             8517..9935
                     /locus_tag="WJK66_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ7578838.1"
                     /translation="MNIHALLSKQWTLPPFLPKRLLLSLLILLAPNAMFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPTPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAVCAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGVEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLNFKIK"
BASE COUNT         2283 a         2959 c         2552 g         2237 t
ORIGIN      
        1 ttaatccact atattttttg ttccaaagtc aaaatatgcc gtccgaacat tcgggcggca
       61 gacaaaacgg cactgcccga taaaggcagt gccgttgtcc gtttcaaacc gtgaaacatc
      121 agcccaaatt aaaggcttta tgcaataccc tggttgccag ttccatgtat ttttcatcaa
      181 tcaatacgga aactttgatt tcggaggtgg aaatcatttg gatgttgata ccctcttcgg
      241 cgagcgtgcg gaagattttg gcggctacac cgacgtgcga acgcataccc aaaccgactg
      301 cggagacttt gcacacggtg tcgtcgccat caatagaagc cgcgccgata ctgtcttggc
      361 gttccgacag gatttccaaa gtctgcttgt aatcgccgcg cggtacggta aaggaaaaat
      421 cggttgtgcc ttcgctgccg acattttgga taatcatatc gacttcgatg ttggcatcgg
      481 caaccgcgcc taaaatctga taggcaacgc cgggtttgtc gggtacgccg cgcacgttga
      541 tgcgggcttg gtttttgtcg aatgcgatac cggttacggc agctctttcc atattgtcgt
      601 cctcttcaaa ggtaattaag gtgccgttgc cgccgtcttg caggctgctc agtacgcgca
      661 ggcgcacttt gtattttccg gcaaattcta ctgaacggat ttgcaaaact ttcgagccaa
      721 ggctggccag ctcaatcatc tcttcaaacg ttaccgtatc catgcggcgc gcttcgggta
      781 cgacgcgggg gtcggtggtg taaacgccgt ccacgtcggt atagatttgg cattcgtctg
      841 ctttgagggc tgcggcgagc gcaacggcgg aggtgtcgga gccgccgcgt ccgagcgtgg
      901 aaatgtcgcc ttcgctgctg atgccttgga aaccggcgac gatgacgact ttgccggcgg
      961 caaggtcggc acgcattttt tcgtcatcaa tgctttcgat gcgggctttg gtgtgggcgg
     1021 tatcggtttt gagggcgacc tgccagccgg tgtagctttt ggcttccacg ccgatgtctt
     1081 tcaatgccat cgccaaaagg ccgatggtta cttgttcgcc ggtggacaaa accacgtcca
     1141 attcgcgcgg atcgggatgc tcctgcattt cgtgcgccaa cgcgaccagg cggttggttt
     1201 caccgctcat ggcggatacg acgacgacga tgtcgtgtcc ttcggcgcgg gctttggcga
     1261 cacgtttggc cacgttttta atgcgttcgg gcgagcctac ggatgtgccg ccgtatttat
     1321 gtacgattaa cgccatgttt cgtgctttct tgtgtgggat tggtcgggca gcatggtttg
     1381 ctggaaaagc ggctattatt actatttttt acatgaagtt caagaacgga ctgcgctttc
     1441 ccgcctgccg tttgacagcg gtcagcgaaa aacctgttct ttcagattgt tgacaaaatg
     1501 ccgtctgaac ggttttcaga cggcatccgg acgacaatca ggcggcggac aacgcatttt
     1561 gctggtgttg cagcagttcg cctatgcctt tttgcgccag tgcaaccagt ttgcccaatt
     1621 cgtccaaact gaacggcgcg tcttccgccg taccttggat ttcgatgatt tttcccgatg
     1681 cggtcatgac gatattcaca tcactgtcgc aaccggagtc ttcgggataa tccaaatcca
     1741 aaagcggcac gccgttcact acgcctactg acacagcggc aacggcttcg cggatggggt
     1801 tttcactcaa aatgccgtct gaaaccagtt tgccgacggc gatttgcagc gcgacaaacg
     1861 caccggtaat cgaagccgtg cgcgtaccgc catctgcctg aatcacatcg cagtcaatca
     1921 agatttgtcg ttcgccgagt ttttccatat ctacgaccgc gcgtagcgaa cgcccgataa
     1981 ggcgttggat ttcttgtgtg cgcccggact gtttgcccgc cgaagcttcg cggcgcatcc
     2041 gggaagcagt tgaggcaggc agcatcccgt attccgccgt tacccagcct tggtttttac
     2101 cgcgcagaaa cggcgggacg ttttcatcta tggaagcggt acaaatcact ttggtattgc
     2161 cgcattcaat aaggcacgaa ccgtccgtat gcggcaggaa atgaggggtg attttgatat
     2221 cgcgcaggct gtccgcggcg cgcgagatgc ggatgtaatc aggcatactg ccctcccgtt
     2281 aaaaacagat aaattaaaaa gccttaaata tgaaaaatca catttaaggc cttcaaactg
     2341 aaaatttcta cgcctcttcg gctttgctgc ggataatcaa aagcggcagg tggctttggc
     2401 gcattaccgt ttcggcaaaa ctgcccatta aaaggtgcat cagtccggta cgtccgtgcg
     2461 tacccaacac cagcaggtcg gcaccgtttt catcggcata atcaaccaaa tcctgcgcca
     2521 tttcacgcgc acccttattg gcaaccagca ggtgtttgac ggtattttcc acacccagtt
     2581 cccgggcggt gcgctcggcg gcatccaaaa cttcgttgcc ttgcgcgacg gcggcggctt
     2641 cgtagctttc gtgttgcaaa aattcggggg cgagcgccat atattcggca ggattggcaa
     2701 cgtgtaccaa agtcagacgc gcaccgttga ccccggcaag ctcggcggca tgtttcaggg
     2761 cattgatgga cgtttcactg ccgtcaacgg caacaaccag atgtttgtac atatcgtatt
     2821 ctccttttgc accgcctcgc ggtgccctct tgtcggatgg gcgcagggac agtttgcgcc
     2881 gtttcattat agacccgctg tcgggcttta tacaacagcc gaacagcccg accgctttcc
     2941 agtataatat gccgcttccg tgcagtcagg cattttttgc cggctttcgt tcactttttg
     3001 atttgacgca atcttgcagg attcgaccat gtccgacaac gctttgacct cttcgcgacg
     3061 cttcggcggc atcgccagac tctacggaga ctctgccttg gcgcactttt cacaggcaca
     3121 cgtctgcgta gtcggcgtgg gcggtgtcgg ctcgtgggcg gtcgaggctt tggcgcggac
     3181 gggcatcgga cgtttgactt tgattgattt ggacaacgtt gccgaatcga atgtcaaccg
     3241 ccagctgcac gccctgaccg gcgacttcgg caaagcaaaa gttaccgcct tgcgcgaacg
     3301 cgtcgcccaa atcaacccag gatgccaagt gtttgaaatt gaagatttcg ttaccgaaga
     3361 caatttgccg gaatacttcg gaaaaggttt tgatttcgtc atcgacgcga tcgaccaagt
     3421 gcgcgtcaaa gcagcaatgg cggcttattt tgtggaacgc aaacaaccgt ttgtcctcag
     3481 cggcggcgcg ggcggacaaa aaaatccggc gttaatccaa accgccgatt tgagccgcgt
     3541 aacccacgac ccgctgcttg ccaacctgcg ctacaccttg cggaaacgct acggattcag
     3601 ccgcgatacg aaagcaaata tgcgcgtgcc ttgcgtgtat tcgaccgaaa atatcgtgcc
     3661 gccgcagtct agggaggctt gttcggcaga tgccgctccg caaggcttgt cgtgcgccgg
     3721 ctacggtgca agcatgctcg ttaccgcttc gttcgggcta tattgcgcac aggcggcggt
     3781 ggaacacatc gcagacaaaa aataagcaat gccgtctgaa acaggattca gacggcattt
     3841 gaacaaacgc ggcaatcaag ctgtcggttc agaaaaacca acatcggcac gcctaaaacc
     3901 gtattcacaa cctgctcctt ttcaaaacat ttgcatttaa aagccgttat aatgccgtct
     3961 gaacatctgc ccgaccacat tatgcgtgaa tgtcggcaga ttgttttctt ttgtaaactt
     4021 atattaaaat ccacttaccg attcacgcca tgccgcccat ccctgcccca tccgcaccat
     4081 ccgagcacac tgtcgcatgg gtattcggcc aacccgttac cgatttgccg caggatttgt
     4141 ttatcccgcc cgacgcgctg aaagtcgtat tgggcagttt tcaaggccct ttggatttgc
     4201 tgctctacct tatccgcaag cagaacatcg atgttctcga tattccgatg gtgaagatta
     4261 ccgagcagta tctgcactac atcgcccaaa tagaagccta tcagtttgat ttggcggcgg
     4321 aatacctgct tatggcggcc atgctgattg aaatcaaatc gcgcctgctg ctgccgcgta
     4381 ccgaaaccgt cgaagacgaa gaagccgacc cgcgtgccga gttggtgcgc cgcctgctgg
     4441 cttacgaaca gatgaagctg gcggcgcagg gtttggacgc gctgccccga gccggacggg
     4501 atttcgcgtg ggcttacctg ccgctggaaa ttgccgtcga agccaagctg cccgaagtct
     4561 atattaccga cttgacgcaa gcgtggctgg gtattttgtc tcgggcaaaa cacacgcgca
     4621 gccacgaagt aatcaaagaa accatctccg tgcgcgcgca aatgacggca atcctgcgcc
     4681 gtttgaacgg acacgggata tgcaggtttc acgacctgtt caatcccgaa cagggcgcgg
     4741 cttacgtggt cgtcaacttc atcgcactgt tggagcttgc caaagaaggt ttggtcggaa
     4801 tcgtacagga agtcggtttc ggagaaatcc gaatcagcct caatcatgag ggggcacatt
     4861 cagacggcat ttccggcaca cggggcgggc gcgatgtgtt ctaatacgcc ccaagccgcc
     4921 accaaaaatc gggagacacg ccatatgacc ggcatcatac attcgctgct tgacaccgac
     4981 ctctacaaat tcactatgct gcaagtggtt ctgcaccagt ttccgcagac gcacagcctt
     5041 tacgaattcc gctgccgcaa cgcctcgacc gtctatccgc ttgccgacat caaggaagac
     5101 ttggaagccg aactcgacgc gctctgccgg ctgcgcttca cccacgacga actcggctat
     5161 ctgcgctccc tgcgtttcat caaaagcgac tttgtcgatt atctcgaact cttccagctc
     5221 caacgccgct ttgtcgaaat cggcacagac gataaagacc gtctgaacat ccgtatcgaa
     5281 ggtccgatga tacaggcgat gttttttgaa atcttcatcc tcgccattgt caacgaactt
     5341 tacttccgcc ggctggaaac ccctgccgtt atcgaagaag gtgaacgccg gcttcaagcc
     5401 aaagccgcgc gcctcaaaga aatcgccgcc gcacaaaacc ccgacgatcc gcccttcctg
     5461 atttccgact tcggcacgcg ccgccgctac aagctcgcgt ggcaggaaca cgtcatccgc
     5521 accctgctcg aagccgcccc aagcatcgtg cgcggcacca gcaatgtctt tctcgccaaa
     5581 aaactcggca ttacccccat cggcaccatg gcgcacgaat tcctccaagc cttccaggcc
     5641 ctcgacgtac gcctgcggaa tttccaaaag gccgcgctcg aaagctgggt gcacgaatac
     5701 cggggcgatt tgggcgttgc cctgaccgac gttgtcggta tggatgcctt cctgcgcgat
     5761 ttcgacctct atttcgccaa acttttcgac gggctgcgcc acgacagcgg cgacccttac
     5821 gtttggggcg acaaagccta cgcccactat caaaagctca aaatcgacag ccgcaccaaa
     5881 atgctgacct tctccgacgg gctggacatc gaacgctctt gggcattgca ccaatatttc
     5941 aaagaccgct tcaaaaccgg cttcggcatc ggcaccaacc tcaccaacga tatggggcat
     6001 acgcccttga atatcgtctt gaaactggtc gaatgcaacg ggcagtccgt cgccaagctg
     6061 tccgactctc cgggcaaaac catgaccaac aacagcacct tcctcgccta cctgcgccaa
     6121 gtgttcgacg tacccgaacc cgaaacgccg taaaccggca gaaaaagcgc acaattcctg
     6181 tttctgccgc ataaaatctt ttaaaatacc gcctgatttg aatttaaccg aaagaccgaa
     6241 cttcatgaac ctacatcaaa ccgtcgaaca cgaagccgcc gccgcctttg ccgccgcagg
     6301 catcgccgac agtcccgttg ttttgcagcc gaccaaaaat gccgaacacg gcgatttcca
     6361 aatcaacggc gtgatgggtg cggcgaaaaa agccaaacaa aacccgcgcg aactggcgca
     6421 aaaggtcgcc gacgcattgg caggcaacgc cgtcatcgaa agcgcggaag tcgccggtcc
     6481 cggttttatc aacctgcgcc tgcgccccga atttctcgcg caaaacattc agacggcctt
     6541 gaacgacgca cgtttcggcg tggcaaaaac cgacaaaccg caaaccgtcg ttatcgacta
     6601 ttcttcgccc aatctggcga aggaaatgca cgtcggccac ctgcgttcca gcatcatcgg
     6661 cgacagcatt tcgcgcgtgt tagaatttat gggcaatacc gttatccgtc aaaaccacgt
     6721 cggcgactgg ggtacgcagt ttggcatgtt ggtcgcttat ttggtcgagc agcaaaaaga
     6781 caatgccgcg ttcgagttgg cggatttgga gcagttttac cgcgccgcca aagtgcgctt
     6841 tgacgaagac cctgcctttg ccgacaccgc acgcgaatac gttgtgaagc tgcaaggcgg
     6901 cgatgaaacc gttttggcat tgtggaaaca gtttgtcgat atttcgctct cgcacgccca
     6961 agccgtttac gacacgctgg gcttgaagct gcgccctgaa gacgtggcag gcgaatcgaa
     7021 atacaacgac gatttgcaag ccgtggtcga tgatttggtt caaaaaggct tggcggtcga
     7081 agacgacggc gcgaaagtcg tgttcttgga cgagtttaaa aacaaagaag gcgaacccgc
     7141 cgcatttatc gtgcaaaaac aaggcggcgg cttcctttat gcttccaccg atttggcgtg
     7201 tctgcgctac cgtgtcggca cgcttcatgc cgaccgcctg ctgtacgtcg tcgaccaccg
     7261 ccaagccctg cactttgagc aactctttac cacttcacgc aaagccggct atctgccgga
     7321 aaacgtcggc gcggcatttg tcggcttcgg cacgatgatg ggcaaagacg gcaaaccgtt
     7381 caaaacacgc agcggcgaca ccgtgaaact ggtcgatctg ctgaccgaag ccgtcgaacg
     7441 cgccaccgct ttggtgaaag aaaaaaatcc cgaattgggt gcagacgaag cggcaaaaat
     7501 aggcaaaacc gtcggcatcg gcgcggtcaa atacgccgac ttgagcaaaa accgcaccag
     7561 cgattatgtg ttcgactggg acgccatgct ctcgtttgaa ggcaacaccg ccccctatct
     7621 gcaatacgcc tacacccgcg tgcaaagcgt gttccgcaaa gcaggcgaat gggacgcgga
     7681 cgcgccaacc gttttgaccg aacctttgga aaaacaactg gccgccgagc ttttgaaatt
     7741 tgaaaacgtg ctgcaaagcg tggcggacac ggcgtatccg cactacctcg ccgcctacct
     7801 ctatcaaatc gctaccttgt tcagccgctt ctacgaagcc tgcccgattt tgaaagccga
     7861 aggcgcaagc cgcaacagcc gcctgcaact ggcgaagctc accggcgaca cgctgaaaca
     7921 aggcttggat ttgctgggca tcgatgtgtt ggacgtaatg taaaaccgca ccgcccgatt
     7981 gcggacaaca gccccgcaat caaccggcgg accatcctta tccgaatctg aaaaaagcgg
     8041 cgcgatacac cgtatccgcc gcctcccaaa atgcgaaaca gacaaacgcc aagcaagcaa
     8101 gcaagcaagc aagcaagcaa gcaaaaaatt ataaccccct tcctgccgac gcacgcactt
     8161 tccgcgcggc gcgttcccct tttcccgctc ctcaaacccg ccttttcttc aggcagggtt
     8221 tcagcccgcc tcctttccct gttttccttc cctcaacacg cgtgcgcgcc ccctgccgca
     8281 ccgtgccgca ctttcgcgcc cggacggcat cggtctgtca tccggttctc tgttttaaat
     8341 acccctgttt cagaaagaaa ttgcagatgt ttcaacacac aggacgacac ataaagcacc
     8401 gccctatgtg ttgccctgat ttggaagggg ttgcgcccct cccaaataaa gtctgatcct
     8461 gccgccccga aggacagatg tccgagtggc ggggtttcaa ccgaaaagga aatacgatga
     8521 atattcacgc cctgctctcc aaacaatgga cgctgccgcc attcctgccg aaacggctgc
     8581 tgctgtccct gctgatactg ctggccccca atgcgatgtt ttgggttttg gcactgctga
     8641 ccgccaccgc ccgcccgatt gtcaatttgg actaccttcc cgccgcgctg ctgatcgccc
     8701 tgccttggcg tttcgtcaaa attgccggcg tattggcgtt ttggctggcg gttttgtttg
     8761 acgggctgat gatggtgatc cagcttttcc cttttatgga cctcatcggc gccatcaacc
     8821 tcgtcccctt catcctgacc gcccccaccc cttatcagat aatgaccggg ctgttgctgc
     8881 tgtatatgct ggcgatgccg tttgtgttgc agaaagccgc cgccaaaacc gacttccggc
     8941 acattgccgt ctgcgccgcc gttgtggcgg cagccggcta tttcaccggc catttgagtt
     9001 actacgaccg ggggcggatg gccaatatct tcggcgcaaa caacttctat tacgccaaaa
     9061 gtcaggcgat gctctacacc gttagccaga atgccgactt tattaccgcc ggcctggtcg
     9121 atcccgtctt cctccccttg ggcaatcagc agcgtgccgc cacgcatctg aacgagccga
     9181 aatctcaaaa aatcctcttt atcgtcgccg aatcttgggg gctgccggcc aatcccgaac
     9241 ttcaaaacgc cacttttgcc aaactgctgg cgcaaaaaga ccgtttttcg gtttgggaaa
     9301 gcggcagttt tcccttcatc ggcgcgacgg tcgaaggcga aatgcgcgaa ttgtgcgcct
     9361 acggcggttt gcgcgggttc gcactgcgcc gcgcgcccga cgaaaaattt gcccgctgcc
     9421 tccccaaccg tttgaaacaa gaaggttatg ccacctttgc gatgcacggc gcgggcagtt
     9481 cgctttacga ccgcttcagc tggtatccga gggcgggctt tcaagaaatc aaaaccgccg
     9541 aaaacctgat cggtaaaaaa acctgtgcca ttttcggcgg cgtgtgcgac agcgagctgt
     9601 tcggcgaagt gtcggcattt ttcaaaaaac acgacaaggg actgttttac tggatgacgc
     9661 tgaccagcca cgccgactat cccgaatccg acattttcaa ccacaggctc aaatgcaccg
     9721 aatacggcct acccgccgaa accgacctct gccgcaactt cagcctgcac acccaattct
     9781 tcgaccaact ggcggattta atccaacgcc ccgaaatgaa aggcgtggaa gtcatcatcg
     9841 tcggcgacca tccgccgccc gtcggcaacc tcaatgaaac cttccgctac ctcaaacagg
     9901 ggcacgtcgc ctggctgaac ttcaaaatca aataacaaca atgccgtctg aacgcaccaa
     9961 cagccttcag acggcatttt gcagacagac cgacccttca agcccacttt tttcatcatc
    10021 tccgataaat t
//
LOCUS       DAWKOG010000060         9978 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.060, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000060 DAWKOG010000000
VERSION     DAWKOG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9978
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.060"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(108..1781)
                     /gene="recN"
                     /locus_tag="WJK66_001118"
     CDS             complement(108..1781)
                     /gene="recN"
                     /locus_tag="WJK66_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEZ7578839.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPVLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSRAQAET
                     VRQLYQNWANSKKALQEAQKQAENIALEQERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGRSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRVEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            complement(1966..2568)
                     /locus_tag="WJK66_001119"
     CDS             complement(1966..2568)
                     /locus_tag="WJK66_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEZ7578840.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLIEMPPTDEEEPTTVPSDTLPN"
     gene            complement(2669..3610)
                     /gene="ylqF"
                     /locus_tag="WJK66_001120"
     CDS             complement(2669..3610)
                     /gene="ylqF"
                     /locus_tag="WJK66_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEZ7578841.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKIWLEHYNSRPDTCAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            complement(3691..4545)
                     /gene="rapZ"
                     /locus_tag="WJK66_001121"
     CDS             complement(3691..4545)
                     /gene="rapZ"
                     /locus_tag="WJK66_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEZ7578842.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIASLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIERFVTHWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLSDR"
     gene            complement(4526..5488)
                     /locus_tag="WJK66_001122"
     CDS             complement(4526..5488)
                     /locus_tag="WJK66_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEZ7578843.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQHNEDRPD"
     gene            complement(5490..5939)
                     /gene="ptsN"
                     /locus_tag="WJK66_001123"
     CDS             complement(5490..5939)
                     /gene="ptsN"
                     /locus_tag="WJK66_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEZ7578844.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLFHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLVFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            complement(6124..7014)
                     /gene="ubiA"
                     /locus_tag="WJK66_001124"
     CDS             complement(6124..7014)
                     /gene="ubiA"
                     /locus_tag="WJK66_001124"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEZ7578845.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGALLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVAGNVLPQAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            complement(7044..7520)
                     /locus_tag="WJK66_001125"
     CDS             complement(7044..7520)
                     /locus_tag="WJK66_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEZ7578846.1"
                     /translation="MEHLFGKWLPDLPAAVSDGIDLPMSRLLKARSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDGIPVVEARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSGEGCGRYFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            complement(7535..8182)
                     /gene="bioD"
                     /locus_tag="WJK66_001126"
     CDS             complement(7535..8182)
                     /gene="bioD"
                     /locus_tag="WJK66_001126"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HEZ7578847.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGMLAKQLLQQGKSVITQKPVQTGC
                     QNIADDIAVHRKIMGKPMQEADKQGLTMPEIFSYPASPHLAARLDGRALDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTEDLLTIDYIQQQDYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLHSLVFNHIHDSRDAHIAQDSLSYLKCRLKADFSEAEWMELAKTDAV"
     gene            complement(8179..9480)
                     /gene="bioA"
                     /locus_tag="WJK66_001127"
     CDS             complement(8179..9480)
                     /gene="bioA"
                     /locus_tag="WJK66_001127"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEZ7578848.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVETQMKQMSHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLTAKQNIATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPKSRFDDEWDGADLQPVRALFEVHHADIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELDKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKEHSK"
BASE COUNT         2319 a         2730 c         2503 g         2426 t
ORIGIN      
        1 cacaatcact tgttcaagtg attaagaaag cgtgataata cggatttatc tgagttctgt
       61 ctattgtttt agacttttat ttttagttat tttttaactg attgatttta aaataaacta
      121 tttttcgacg caagttgcag caattctgcc gcatgttgcc gcgtcgtatc ggtaatgact
      181 tctccgccca acatacgggc aacctcttcg acccgttgga tttcatccaa tatactgatt
      241 tcgctgacgg tttgctctcc ctcgctgtgc ttgcgcaccc gccagtggtt ttcgccgcag
      301 gatgcgactt gcggaagatg ggtaacggca agcacctgat gttttctgcc caacgcacgt
      361 aatgccttgc cgaccatttc agccactccc cctccaatac cggtatcgac ctcatcaaaa
      421 atcagggtgg gaacttgggt atattggctg gcaacaacct gtaaggcaag gctgatacgc
      481 gccaattcgc cgccggaggc aactttattc agcggacggg gcggattgcc tttgttggcg
      541 gcaacttgaa attgaacctg ctccaaaccg tgtgccgtcg gcgacgaggg caacaggacg
      601 atgtcgaaac gtgcaccttt catggcaagg tgttgcatat gctcggtcgt ttcgccgctc
      661 aaacgctctg ccgcctgatg gcgcatggca gaaaggatgt gggcagcttc ctgatattcg
      721 gcaaaattgt gggcaacatt atgctcgagc gcgtccaaat cggcggcagc ttgcaggctt
      781 tgcaggcgtt cttcgatttc tgccaacttg gcaggcaact cttcaggctc gatccggtat
      841 ttccgcgcca tccccatcag ttcgcccata cgctgttcct gtgccgccaa ttcattgggg
      901 ttgatgtcgc tgcgacctgc cacatcgcgc atattggcac tgatttcgcc caattcggct
      961 tcgatgcttg ccaacatatt caggctctcg gcaaagcgcg gctcgatgtt ttgcagattg
     1021 gccaatagtt tttgacattg atagatatgg cgttggatgc cgttgtcgcc gtcaatcttg
     1081 cttccgactt cttcggcagc ctgcaacagc tcggcagaat gggcaaggct gtcgtggctt
     1141 tggctgaggg cttcccactc gccttgttta atgtccaact gattcaattc gttaaactgc
     1201 cattccagac gctcttgttc gagtgcaatg ttttctgcct gtttctgcgc ttcctgcaat
     1261 gctttttttg agtttgccca gttttgataa agctgcctga cggtttccgc ctgcgccctg
     1321 ctgcccgcaa atgcgtccaa caattcgcgc tgggcggctt cctgattaag cgaatgatgg
     1381 gcgttttgcc catggatgtc gataagctgg ctgccgacgg ctttgagttg cgccaaggta
     1441 gcggcctgat tgttgataaa gctgcggctt ttgcctttgg catcgataat gcggcggata
     1501 ctgagttctt cttctccgtc gtttaaaagc ccctgttcat acaattctgc ttttaaaaca
     1561 ggtaaatggg aaatatcaaa caacgccgac aactgcgctt cttttgcgcc gctgcggact
     1621 tggctgtaat cggctttatc gcccaacagc agaccaatcg catccaaagt aatggacttg
     1681 cccgcgccag tttctccggt caatacggta aagccgcttt gaaaatccag attcagattt
     1741 tcaacaatga caaaatcacg caaagaaagt gttagaagca tcgtgaggat tccttagcaa
     1801 ctctaccgac aaaagcaata taaattttag atatacgaat tataactaag atttttttaa
     1861 attaaaaata agtttttcct taatttttct tagtgcttgt ttttatctgc ttatttgttg
     1921 caatgccgtc tgaagcaatg tgcgtttcag acggcatttg gaatttcagt tgggcagggt
     1981 atcggacggt acggttgtcg gctcttcttc atccgtaggc ggcatttcta tcaaatcggg
     2041 caaaaccagt tcgcccagtt cggtcagcgg aggaagttct tccagaccgt ccaagcgcaa
     2101 atcgctgaga aacgttgccg ttgttgccca caatgcgggt tttcccaatg tgtcccgatg
     2161 tccgatgact tcaatccacc cccgatcctg caaggtctgc atcacgttct gcgacaccgc
     2221 cacgccgcgt ataccttcga tgtcgccgcg cgttacgggc tgctggtagg cgataatcgc
     2281 cagtgtttcc atcacggcgc gggagtagcg cggcgcacgc tgttcttgca gactgcccag
     2341 ccgctcgaat gccgtctgaa caatctgaaa acgccagccc tcttgcgtat gcaccagttg
     2401 caacgcccta tcctgccaac gcgttttcaa ctgcgccaac acatcaatca gtttgtcctg
     2461 cgacagtggc ggcacacaca attcgcgcat agatttttcg gtcagcggtt cggtttgggt
     2521 cagcagtgcg gcttcaatca gcgcgtcggg agaaattttg tcggtcatac gggtatccgt
     2581 gctgaaacgg catgggtttg gatatgccgt ctgaaatcgg ttggagtaga gagaagctgc
     2641 ctgaaaaata tttttcagac ggcattcttt atgcttccga aggcttctgc cctttacgct
     2701 ctgcttttct ggcttcgcgt atggctttga gttcggcttc tttctgacgg gcttttttga
     2761 gccaagtttc ccattggttc ggcgtttcga gggtgattct gccgattttg ccttcacgga
     2821 agtcggtgag gatgttttcg gcggcttttt ggtagttgat ccgtccaccg ctgaggactg
     2881 cgccgcgttt tttggctatc cattcgagcc aaacgttttc gtcccagtgg ctgctggggt
     2941 ctttgtcggc ttggtagcgt tcttgcagca tagggaggta gtggcggcgg aggtagtcta
     3001 aaagttcgag ggcgacttct tcttcgtcca atgcgttgcg tccgactgcg ccgccggcgg
     3061 cgaggttgta gccgctttct tcgacaatga ttttcggcca cagcattccg ggggtgtcgt
     3121 acagccagaa gtcgtcggca aggaaaaggc gttgttcggc tttggtgatg ccgggttcgt
     3181 tgccggtttt ggcggatttt ttgcctatca tgccgttgat gagggtggac ttgccaacgt
     3241 tggggatgcc gcagatgagg acgcgcaggg gtttatctat gccttggcgg tggggaatca
     3301 tggcacgaca ggcttgggta attttgccgt gtgcgcctgt ttcggaggaa tcgagggcga
     3361 tggcgcaggt gtcggggcgg ctgttatagt gttcgagcca gattttggtg cgctcggggt
     3421 cggcaagatc ttgtttgttg aggattttaa gtttaggttt gcctttggaa agttgggcaa
     3481 gcagggggtt ttcgctggag gcgggcattc gcgcatccag catttcaatc accatgtcta
     3541 cgcttttggc gcgctcggcg atggcttttt tcgctttgtt catatggccg ggaaaccatt
     3601 ggattgccat gtctttcctt tcttttcaat atttgaaatg ccgtctgaaa cggaggacgg
     3661 ggtttcagac ggcataatgg tttgacggaa ttagcggtct gacaggtttt gcgcctgtct
     3721 gtgccgtatc agcaattcat aacgcccttt caaccttcgg gcgagttttt cgacaatata
     3781 gaccgaacgg tgctgtcctc ccgtgcaacc gatggcgacg gtaacgtagc tcctgctttc
     3841 atcctccaaa cgcggtaacc aatgcgtaac aaacctttcg atgtcgtcaa ccatttcctg
     3901 cacaagcggc tgtccgtcca aataatccca aacgggcttg tccataccgg tgtaaggcct
     3961 caactcggga tcgtaatacg ggttgggcag gctgcgcata tcgaacataa aatccgcgtt
     4021 gttcggcaca ccgtatttga acccgaagga ctccaaaatc accagcagcc cggtacgttc
     4081 gaccttcagc cactgccgga ctgcatggcg gagctgttgg gcattcatct tggaagtgtc
     4141 gatacaatag gcgatttctt taagcgggaa cagccattcc cgttctttct ttaagctttc
     4201 caacaaggtc atatcctgat tgctcagagg atgtcctcgc ctggtttcgg aaaaccggcg
     4261 gaccaacacg ctttcttccg cctcgacaaa caaaacttca accctgtgcc ccagtccgcg
     4321 cagagaggca atctgttccc gcgcctgtcc gatgtcaatg ccggaacgca catcgacgct
     4381 gaccgccaat tcggtttcgt ccgcacgttc gatatgatac gacaccagcg cgggcaacat
     4441 ttccaaaggc aaattgtcca cgcagaaata acccgaatct tccatttggc gcagtgcgac
     4501 ggacttgccc gaaccggaca ggccgctaat caggacgatc ttcattgtgt tgttcgtttt
     4561 ctttaagttg cgtctgatgg cgttccaaaa attcgcgcgt actgtcctta ccgcgcaact
     4621 gcaaaatgta attgcgtacc gccgcctcaa ccaaaacggc gaggttgcgt ccgacggcga
     4681 cgggcagcgt aaccgaacgg acgttgacgt tgaggatgga ttcggtttcg gtgcggatgc
     4741 tcaaccggtc aagctgcttc atatactcgt cgtccgcctc gactaaattg ataatgagtt
     4801 gcaggatttt tttggggcgg atggaagttt cgccgaaaat atggcggata ttgagtatcc
     4861 ccaagccgcg cacttccaaa aaatcgcgca gcataggcga acaacgccct tccagcgttt
     4921 ccgggccgat gcggaacagc tcgaccgcat cgtcggcaat caggctgtgg ccgcgcgaaa
     4981 tcagttccaa tgccaattcg ctcttaccca ggccggaatg cccggtaatc agcacgccga
     5041 tttcaaacac atcgagaaat acgccgtgtt tgacggacga tgccgccaag gtgcgttgca
     5101 ggtaaatccg caacacgtcc atcagatagg ggctttcgag tttggaagtc agcagtggaa
     5161 tatcgttttt atgacaatag tcgcgcagtc ccggggaaac cggcaagccg tttgccacaa
     5221 tgaccaaaga catggaaata tcgaacaggt ctccgaactg ataacccgtt tcccccgatt
     5281 cgagacggtt cagatattcc gactctgcca aaccgaccac ttggatttgg ttggggtgga
     5341 taaaattcag gtgtccgacc agggcgagga cgggcttgtc cgcctctaca ccgatacggt
     5401 tgtccgcacc cgaattgccg gcggcccagg caagttgcag tttgtattgg ttgtcgtcaa
     5461 acagccggcg gacagagata ctgggcatat tattcttcag tcaggatggc gcgggcttct
     5521 tccgcagaag aaaccgtcat cagcgattct ctgatgcttt tttgggaaaa cttgccggcc
     5581 agtttggaca atacttccaa atgctcgccc gtcgcatttt ccggaaccag caagataaaa
     5641 accagggaaa ccggtttgcc gtccggtgcg tcaaatccga cgggttcgcg cgtgcggatg
     5701 aacgcgcccg tcgcctgctt cacgccggca tgacggccgt gcgggatggc aacgccctgc
     5761 cccaaaccgg tcgaaccgag tttttcacgg gcaaaaagac attcgaaaac atcagcatgg
     5821 aacaatgagg attcgcgttc caaaagcagg cccgcttcct caaacagcct ttttttactg
     5881 cccacctcca tatccaaaac aatatgggac aaaggcaaaa tttcgccgat aaggctcata
     5941 agcttctctt ttcagacatc gcaaaacaga aagattgtac cgactgccag ggcaaatctc
     6001 aatcccgcat acggtacggg ctgacataac acagcgtttt aaaaacatat tttaacgctt
     6061 ttcggcacag atagaaatgc cgtccaaagc agtttacggc tcttcagacg gcattgccct
     6121 gccttatttc gcgaaaaacg tatgggcaaa aatggcggta aaccacaccc aaccaattct
     6181 gttgtttgcc aaaaacgttt caaaacagat ttgccggacg cggcttttga tggcggcata
     6241 ttggcggtat tgcagcaaca ggacgatggg gattgccgtc caatatgccc atgccgcacc
     6301 gataaccgca cccaatactg ccatcagcag ggtaaagcct ccgtgacaca gcataacggc
     6361 ggcgatgtcg taacgcccga acgtgacggc ggaggttttg atgccgattt tcaaatcgtc
     6421 ttctttgtcc gccattgcat aaaccgtgtc atacgccaga gtccataaca cattggcggc
     6481 aaagagtatc cacgcttgag gcagcacgtt tccggcaacg gcggcaaacg ccatcgggat
     6541 accgaaggaa aaggcaagcc cgagatagag ttggggaatc ggaaaaaaac gtttggtaaa
     6601 cgggtaagtc agcgcaagaa acagcgcggg caggctcatc agccaagtca gatgattcag
     6661 cggaatcagg cacaatgcgg caagcaggca caaaaatgcc gtcagcagca gcgcttcttt
     6721 tttcttgacc ctgccctgtg cgaacggacg gtttttggta cgctcgacag caccgtcaaa
     6781 atcgcggtcg gcaaaatcgt tgatgacgca accggcactg cgcattaaaa acgtaccgat
     6841 tgtaaacgcc gccaataccg ccaaatcggg aatgccgtct gaagccagcc acaatgccca
     6901 gtaggtcggc cacagcaaaa gcagcgcccc aatgggcttg tccgcccgca tcaggcgcag
     6961 gtacacatcc aaacggtcgg acagtcgtaa aaataaaggg gatttaggat tcatattgcc
     7021 gcacagcttg aaaaaacggt attttatccg ataaagcgtt tcagttcggg cagaaaatac
     7081 tcggtcagca gcatttcctc gccgtgacgg gaaaaccgag aacgccgcgc ggcaaagtac
     7141 cgtccgcatc cttcgccgga aacggcaaac tcaaacgccg aacgcgcccc ttccaaatcg
     7201 gcttgaaaca gacgctcgcc caaagggcgc gtgccgcagt ccaaaatgtt ttgccaaaac
     7261 gccgaaccta tacggcactc gctccttgcc tcaacaacag ggataccgtc cagcttcaac
     7321 aaaacttcgc gcaccagcct ccctccgcat tccgtctcca attcgcccag tttcagcagt
     7381 tccaccgaaa atgtatgcgg caaggcgcgc aatgcggcgg tcagcgaccg ggctttcaat
     7441 aaccggctca tcggcaggtc gatgccgtct gaaacggcgg cgggcaagtc gggcagccat
     7501 ttcccaaata ggtgttccat atttttccca atctttatac cgcgtctgtt tttgccaact
     7561 ccatccattc cgcttcggaa aaatcggctt tcagacggca tttcaagtag ctcaggctgt
     7621 cttgggcgat gtgtgcgtcg cggctgtcgt ggatgtggtt gaacacgagg ctgtgcaagc
     7681 gaatgccata ttgtttgagc gcggcgaaac tgagtaaagt gtggttgata ctgcccagcc
     7741 gtccgctggt aacgaggatg acgggataat cttgttgctg aatataatca atggttaaca
     7801 aatcttccgt cagcggaacc atcaatccgc ccgcgccttc gaccaaaacg acttcgtact
     7861 gcgccgccaa ttcttgtgtg gcggtgcgga ttttgtccaa gtccaaagcc ctgccgtcaa
     7921 gacgggcagc gagatgaggc gaagctggat aactgaagat ttcaggcatg gtcagccctt
     7981 gtttgtcggc ttcctgcatg ggtttaccca tgattttacg atggacggcg atgtcgtcgg
     8041 caatgttttg gcaaccggtt tgcacgggct tttgcgtaat cacgcttttg ccctgctgca
     8101 acaattgttt tgccaacata ccggtggcga cagttttgcc gatgtccgtg tctatgccgc
     8161 tgacgaagta aacgcctttc atttgctgtg ttccttcaag atttgcacgg ttttgtcggc
     8221 aagtttggtc aaaacgccgt ctgaaataat atacggcggc atcagataca ccagcctgcc
     8281 gaacgggcgc acccaaatgc cctgcgccac gcaatctgct tgaaaacgcg ccatatccac
     8341 gcctttatcc agctcgatca cgccgatggc acccaaaacg cgtacgtctt tcacgccgcg
     8401 aatatcccac gcggctttca gacggccttt taagatgctt tcaatgcggc ggatatttgc
     8461 ctgccagtct tgagacaaaa gcagtttgac cgaagcgcag gcaacggcac acgccagcgg
     8521 gtttgccata aacgtcgggc cgtgcataaa cacgcccgct tcgccgcgcg aaatcgtttc
     8581 ggtaactttt tgcgaagtga ttgctgccgc cagcgtcata tagccgccgc tcaaaccctt
     8641 gccgatgcac ataatatccg gcacgacctc cgcgtgttcg caggcaaaca tcttgcccgt
     8701 gcgcccgaat ccggtggcga tttcatcaaa aatcaaaacg atatcaaact cgtcgcacaa
     8761 atcgcgcaat ccgcgaagat actgcggatg ataaaaatac atgccgcccg cgccctgcac
     8821 gaccggctct aaaataaagg cggcaatatc cgcatgatgc acttcaaaca aggcgcggac
     8881 aggctgcaaa tccgccccgt cccattcatc gtcgaaacgg cttttcggat tatcgacaaa
     8941 ataacgctgc ggcaacgcgc tgccgaaaat atggtgcatc cccgtttccg gatcgcagac
     9001 ggacatcgcg ttccaagtat cgccgtgata cccgcgccgt accgtcgcaa tattctgctt
     9061 cgccgtcaac ccccgcgcct gctggtattg caccgccatc ttcaacgcga cttccaccga
     9121 aaccgaaccc gaatccgcat aaaaaatacg atccagcccc tgcggcaaaa tcccgaccaa
     9181 caatttgccc agctccaccg ccggctcatg cgtcaaaccg ccgaacatca catgcgacat
     9241 ctgtttcatc tgcgtctcaa ccgcctgatt caaaacggga tgattgtaac cgtgtatcgc
     9301 acaccaccaa gacgacatcc cgtcaatcag ccgcgtgccg tccgccaatt cgataaacac
     9361 cccttctgca cgtttgacag gataaacggg aagcggatcg gtcatggaag tatagggatg
     9421 gagcagatgg gtacggtcga aatcaagcaa tgatgatgtg tgttgatgtt cagacggcat
     9481 aagtttctct cttttcttat tgctgcattc aaacgcaaaa cgcgtattct actccgacag
     9541 accgcttccc acacctttcc atccgtttcg ggcgtaaaac cacgcaacaa atcgtccgca
     9601 gtataagcgc acaccgtttc gcattcccca agcccgattg gaatcagacg acccaacgcc
     9661 caatattgtt tcacacagcc tccgcccaac gcttcaatca gtcatagaca aaacggcaat
     9721 ccatacgata cagatcatag caacagccat cgcaacagcg ttagcaaaat caggggactc
     9781 cgacataggc gcatagcatc taccgatgca cagctcctca ttcggctcta tgaataccat
     9841 acccatcaca aaatccaccg ccaaaaccag gcacggcttc ttatacttat gatagatttc
     9901 caccatcctg ccccatatat cccaaacatt cataccgcat atcccgcagg caacaaattc
     9961 cgattgaagg ttacagcc
//
LOCUS       DAWKOG010000061         9904 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.061, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000061 DAWKOG010000000
VERSION     DAWKOG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9904
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.061"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            109..933
                     /locus_tag="WJK66_001128"
     CDS             109..933
                     /locus_tag="WJK66_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578849.1"
                     /translation="MNRTAFCCLSLTAALILTACSSGGGGVAADIGAGLADALTAPLD
                     HKDKSLQSLTLDQSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIE
                     VDGQLITLESGEFQVYKQSHSALTALQTEQVQDSEHSGKMVAKRQFRIGDIAGEHTSF
                     DKLPEGGRATYRGTAFGSDDASGKLTYTIDFAAKQGHGKIEHLKSPELNVDLAASDIK
                     PDKKRHAVISGSVLYNQAEKGSYSLGIFGGQAQEVAGSAEVETANGIRHIGLAAKQ"
     gene            1073..1750
                     /gene="tsaB"
                     /locus_tag="WJK66_001129"
     CDS             1073..1750
                     /gene="tsaB"
                     /locus_tag="WJK66_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEZ7578850.1"
                     /translation="MQADFNRPVLAVDTGTSYLSLALRADGETRLFHQEVGSRQSALI
                     LPEIRTLFRNAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFDTPLIGIPTLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADIRLPEGCAFSDG
                     IGSAFALEETPPFSGKPDMPTAADFLNLAAEGGFPAVAAAHAELLYVRNKIALTAKEQ
                     AERRARL"
     gene            1747..2187
                     /gene="rimI"
                     /locus_tag="WJK66_001130"
     CDS             1747..2187
                     /gene="rimI"
                     /locus_tag="WJK66_001130"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEZ7578851.1"
                     /translation="MNIRRAVYADCAALAALDAVCNPSAWTQRQFESALVSLSEQVFL
                     AEKDGGLAAFIVWQNLPDESELHLIATAPECRRQGIASALLEYWFAHLPEGTQRLLLE
                     VRAGNTAAQALYAAHGFSIAGRRKNYYRATDGQTEDAVLMEKIC"
     gene            2181..2915
                     /locus_tag="WJK66_001131"
     CDS             2181..2915
                     /locus_tag="WJK66_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEZ7578852.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARPQTV
                     RAAPIRPSQPHNGQARLETMKALETAAVPTRKPAPETETPLPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFHGKAGVLLDNILKAVGLDAAYVHKTSWVKTAAVGNPMP
                     SEQAVANALGQIARELDGCRAPAVLFLGQAFVQPERQTMIETLCGSRPFFIIDHPARL
                     LRQPELKARNWQVLKQLKHALQAGGS"
     gene            2977..3618
                     /gene="pyrE"
                     /locus_tag="WJK66_001132"
     CDS             2977..3618
                     /gene="pyrE"
                     /locus_tag="WJK66_001132"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEZ7578853.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            3692..4126
                     /locus_tag="WJK66_001133"
     CDS             3692..4126
                     /locus_tag="WJK66_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578854.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTLHDTPPATAATP
                     AAAPQVAVPPAAPARQEGFNWTIATLFALIVLIMQLSYLVIL"
     gene            4123..5433
                     /gene="argA"
                     /locus_tag="WJK66_001134"
     CDS             4123..5433
                     /gene="argA"
                     /locus_tag="WJK66_001134"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEZ7578855.1"
                     /translation="MIVPDLFVAHFREAVPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYSGKTFYLDMLQTAASVAVSLQAEKLVYLTLSDGISRPDGTL
                     AETLSAQEAQSLAEHAGGETRRLISSTVAALEGGVHRVQILNGASDGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEISCLAVSPQAQDGGYGERLLAHIIDKARSMGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNSHILVRRLHR"
     gene            5529..7544
                     /locus_tag="WJK66_001135"
     CDS             5529..7544
                     /locus_tag="WJK66_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEZ7578856.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLNNDKARALS
                     DGILNQMQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEKPNRALLTLSKSGGDTAYTLEVDLEARELVEGGFHFP
                     AGKNQVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPVYQIDKDAMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSAEGEAKPLNLPNDCDVVGYLAGHLLL
                     TLRKDWNRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADGKWQEVELPRLPSGALEMTDQPWGGDVVYLAAGDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFDSDGIEVRQFWVMSSDGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGVPELPHYLGSIGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGISSPQHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEVCKRRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSPQSWLYSPDGGGHTGNGTQREAADELACVLLFLKEFLG"
     gene            complement(7658..8614)
                     /locus_tag="WJK66_001136"
     CDS             complement(7658..8614)
                     /locus_tag="WJK66_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7578857.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLEM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDNSGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAAALTAAACPLLQRSFESDGFGLLADEAAALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILQHEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGCLRRLRLEHAL
                     ALIESGSTVQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            8850..9815
                     /locus_tag="WJK66_001137"
     CDS             8850..9815
                     /locus_tag="WJK66_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7578858.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGSRQLIQAAEQLKEAFEKAEPVAAGKE"
BASE COUNT         2173 a         2992 c         2814 g         1925 t
ORIGIN      
        1 aggcaattga aaattttttg gcgcattttt atgcgtcaaa tttcgttaac agactatttt
       61 tgcaaaggtc tcatattatc tattctgcgt atgactagga gcaaacctgt gaaccgaact
      121 gccttctgct gcctttcttt gaccgccgcc ctgattctga ccgcctgcag cagcggaggc
      181 ggcggtgtcg ccgccgacat cggcgcgggg cttgccgatg cactaaccgc accgctcgac
      241 cataaagaca aaagtttgca gtctttgacg ctggatcagt ccgtcaggaa aaacgagaaa
      301 ctgaagctgg cggcacaagg tgcggaaaaa acttatggaa acggcgacag cctcaatacg
      361 ggcaaattga agaacgacaa ggtcagccgc ttcgacttta tccgtcaaat cgaagtggac
      421 gggcagctca ttaccttgga gagcggagag ttccaagtgt acaaacaaag ccattccgcc
      481 ttaaccgccc ttcagaccga gcaagtacaa gattcggagc attcagggaa gatggttgcg
      541 aaacgccagt tcagaatcgg cgatatagcg ggtgaacata catcttttga caaacttccc
      601 gaaggcggca gggcgacata tcgcgggacg gcattcggtt cagacgatgc cagtggaaaa
      661 ctgacctaca ccatagattt cgccgccaag cagggacacg gcaaaatcga acatttgaaa
      721 tcgccagaac tcaatgttga cctggccgcc tccgatatca agccggataa aaaacgccat
      781 gccgtcatca gcggttccgt cctttacaac caagccgaga aaggcagtta ctctctaggc
      841 atctttggcg ggcaagccca ggaagttgcc ggcagcgcag aagtggaaac cgcaaacggc
      901 atacgccata tcggtcttgc cgccaagcag taaccattgt gaaaatgccg tccgaacacg
      961 ataatttacc gttcggacgg catttttgcg ccataccgcc ctaggatacg acggcacggc
     1021 aggcaagccg gtttcactat ataatgccgt ctgaaccaac gagaaccaca ccatgcaagc
     1081 cgatttcaac cgccccgtcc tcgccgtcga taccggaact tcctatttgt cgctcgcgct
     1141 gcgcgccgac ggcgaaaccc gtctgttcca tcaggaagtc ggcagccgcc agtccgcact
     1201 gatcctgccg gaaatccgca ccctattccg aaatgcaggc attaccgccg ccgatttggg
     1261 ggcgattgtt tatgcgaaag gccccggcgc gtttacggga ctgcgtatcg gtatcggcgt
     1321 ggcgcaaggt ttggcaacgc cgtttgatac gcctttaatc ggcatcccca cgctcgatgc
     1381 cgccgcctcg ctgccgccgc cgcaaagctg catactcgcc gctgcggacg cgcgtatggg
     1441 cgaagtgttt tatgcgtggt tcgacacgct gaaccgccgc cgtttgagcg attatcaggt
     1501 cgggcgggcg gcagacatcc ggctgccgga gggatgtgcc ttttcagacg gcataggcag
     1561 cgcgttcgcg ttggaagaaa cgccgccgtt ttcaggcaaa cccgatatgc cgaccgccgc
     1621 cgacttcctc aatctggcgg ctgaaggcgg ttttcccgcc gtcgctgcgg cacacgccga
     1681 gctgctctac gtccgcaaca aaatcgccct gactgccaaa gaacaggcgg aacggagggc
     1741 gcgcctgtga acatccgccg cgccgtttat gccgattgcg cggcactcgc cgcactcgat
     1801 gccgtctgca acccgtccgc gtggacgcaa cgccaatttg agtctgcatt ggtttcgctg
     1861 tccgaacagg ttttcctcgc ggaaaaagac ggcgggttgg ccgcctttat cgtttggcag
     1921 aacctgcccg acgaatccga actgcacctg attgccaccg cgcccgaatg ccgccgccaa
     1981 ggaattgcgt ccgccctgct cgaatattgg ttcgcacatc tgcccgaagg cacgcaacgc
     2041 ctgctgctcg aagtccgcgc aggcaacacc gccgcgcagg cactgtacgc ggcgcacggc
     2101 ttcagcattg cgggcaggcg gaaaaactat taccgcgcca ccgacgggca aaccgaagat
     2161 gccgtcttaa tggagaaaat atgttaagcg cgcgctacct ccacctgcac gaagctttgg
     2221 gtttgggtcc gatgtggctg aaacaggccg ctgccgtcct gccgcccaaa aacacacccg
     2281 caaccccggc acaggcacgt cctcaaaccg tccgcgccgc cccgatccgc ccttcccaac
     2341 cccataacgg tcaggcgcgg ctcgaaacga tgaaagcgtt ggaaaccgcc gccgtaccta
     2401 cgcgcaaacc cgcgcctgaa accgaaacgc ctctgcccgg cctttcagac ggcatcgccc
     2461 ccgttcccgc cgcttcgggc atcaccaagc ttgccgtcgt cagcctttgc ccaccgatcg
     2521 aggatgcggt ttacgggcaa ctgttccacg gcaaagcggg tgtcctgctt gacaacatac
     2581 tcaaagccgt aggactggat gccgcttatg tacacaagac cagctgggtg aaaaccgccg
     2641 ccgtcggcaa cccgatgccg tctgaacagg ccgtcgcgaa tgcgctgggt caaatcgcca
     2701 gggaactcga cggctgccgt gcccccgccg tccttttcct cgggcaggct tttgtccagc
     2761 ctgaacggca aacgatgatt gaaactttgt gcggcagccg tcccttcttc atcatcgacc
     2821 atcccgctcg gcttttacgc caacccgaac ttaaagcccg aaactggcag gtgttgaaac
     2881 agttgaaaca cgccttgcag gcaggcggca gttgaagcag gccgcacggg gcggtagaat
     2941 cgcaactgcg tcccaatatc tgacagaaag cacaaaatga ccgatttccg ccaagatttc
     3001 ctcaaattct ccctcgccca aaatgttttg aaattcggcg aattcaccac caaagccgga
     3061 cggcggtcgc cctatttctt caatgccggc ctctttaacg acggcttgtc cacgctgcaa
     3121 ctggcaaaat tctatgcaca atccatcatt gaaagcggca tccgattcga tatgctgttc
     3181 ggtcccgcct acaaaggtat tattttggcg gcggcaaccg cgatgatgct ggcggaaaaa
     3241 ggcgtgaacg tcccgtttgc ctacaaccgc aaagaagcca aagaccacgg cgaaggcggc
     3301 gtgttggtcg gcgcgccgct taaagggcgc gtgctgatta tcgacgacgt gatttccgcc
     3361 ggcacatccg tacgcgaatc gatcaaactg attgaagcgg agggtgcaac ccccgccggt
     3421 gtcgccatcg cgctcgaccg catggaaaaa ggcacgggtg aattgagcgc ggttcaggaa
     3481 gtggaaaaac aatacggtct gcccgtcgcc cccatcgcca gcctgaacga tttgtttatt
     3541 ctgttgcaaa acaaccccga attcggacag ttcctcgaac ccgtccgagc ctaccgtcgg
     3601 cagtacggcg tagaataaaa acaaagcata tgccgtccga accgccttac gcctcagacg
     3661 gcatcaaacc tgacacacac gaggaaatac catgcccgcc tgtttctgcc cccactgcaa
     3721 aacccgtctc tgggtcaaag aaacccaact caatgtcgcc caaggcttcg tcgtctgcca
     3781 aaaatgcgaa ggactgttta aagccaaaga ccatctggca agcacgaaag aacccatatt
     3841 caacgatttg cccgaggctg tttcggatgt caaactcgtt caccgcatcg gcacgcgcgc
     3901 cattggcaag aaacagattt cccgcgacga aatcgccggc atcctcaacg gcggtacaac
     3961 cctgcacgat acgccgcccg caaccgccgc cacccctgcc gccgcaccgc aggttgccgt
     4021 accgcccgcc gcgcctgccc gtcaggaagg gttcaactgg acgattgcaa ccctgtttgc
     4081 ccttatcgtc ctcattatgc agctttccta cctcgtcatc ctatgatcgt gcccgacctc
     4141 tttgtcgccc acttccgcga agccgtcccc tacatccgcc aaatgcgcgg caaaacgctg
     4201 gtcgccggca tagacgaccg cctgctcgaa ggtgatacct taaacaagct cgccgccgac
     4261 atcgggctgt tgtcgcaact gggcatcagg ctcgtcctca tccacggcgc gcgccacttc
     4321 ctcgaccgcc acgccgccgc gcaaggccgc acgccgcatt attgccgggg cttgcgcgtt
     4381 accgacgaaa cctcgctcga acaggcgcag cagtttgccg gcaccgtccg cagccgtttt
     4441 gaagccgcat tgtgcggcag cgtttccggg ttcgcgcgcg cgccttccgt cccgctcgta
     4501 tcgggcaact tcctgaccgc ccgtccgata ggtgtgattg acggaaccga tatggaatac
     4561 gcgggcgtta tccgcaaaac cgacaccgcc gccctccgtt tccaactcga cgcgggcaat
     4621 atcgtctggc tgccgccgct cggacattcc tacagcggca agaccttcta tctcgatatg
     4681 cttcaaaccg ccgcctccgt cgccgtctcg cttcaggccg aaaaactcgt ttacctgacc
     4741 ctttcagacg gcatttcccg ccccgacggc acgctcgccg aaaccctctc ggcacaggaa
     4801 gcgcaatcgc tggcggaaca cgccggcggc gaaacgcgac ggctgatttc gtccaccgtt
     4861 gccgcgctcg aaggcggcgt gcatcgcgtc caaatcctca acggtgcctc cgacggcagc
     4921 ctgctgcaag aactcttcac ccgcaacggc atcggcacat ccattgccaa agaagccttc
     4981 gtctccatcc ggcaggcgca cagcggcgac atcccgcaca tcgccgccct catccgcccg
     5041 ctggaagaac agggcatcct gctgcaccgc agccgcgaat acctcgaaaa ccacatttcc
     5101 gaattttcca tcctcgaaca cgacggcaac ctgtacggtt gcgccgccct gaaaaccttt
     5161 gccgaagccg attgcggcga aatttcctgc cttgccgtct cgccgcaggc acaggacggc
     5221 ggctacggcg aacgcctgct tgcccacatt atcgataagg cgcgcagtat gggcataagc
     5281 aggctgttcg ccctctccac aaataccggc gaatggtttg ccgaacgcgg ctttcagacg
     5341 gcatcggaag acgagttgcc cgaaacgcgg cgcaaagact accgcagcaa cggacgaaac
     5401 tcgcatattc tggtacgtcg cctgcaccgc tgaccgcaac ggaaagccgc cgcagaaatg
     5461 ccgtctgaac cccgtttcag acggcatttc cccgattata aagacctgcc caaccctcaa
     5521 ggaccccgat gaaatcctac cccgacccct accgccattt tgaaaacctc gattccgccg
     5581 aaacgcaaaa cttcgctgct gaagcgaatg ccgaaacgcg cgcgcgtttt ttaaacaacg
     5641 acaaggcgcg cgcgctttca gacggcattt tgaatcaaat gcaggacacg cggcagattc
     5701 cgttttgtca ggaacaccgc gcgcggatgt accatttcca tcaggacgcg gaatatccga
     5761 agggcgtgta ccgcgtgtgc acggcggcga cctaccgttc gggctatccg gagtggaaaa
     5821 tcctgttttc agtggcggac ttcgacgaat tgctcggcga cgatgtatat ttgggcggcg
     5881 tgtcgcactt ggtggaaaag cccaaccgcg cgttgttgac actaagcaaa tcaggcggcg
     5941 atacggcgta cacgctggaa gtggatttgg aagcaaggga attggtagaa ggtggttttc
     6001 actttccggc aggcaaaaac caagtgtcgt ggcgcgatga aaacagcgtg tgggtgtgtc
     6061 cggcttggga cgaacgccag ttgaccgaat cgggctatcc gcgtgaggtg tggctggtgg
     6121 aacgcggcaa gagtttcgag gaaagcctgc ctgtgtatca aatcgataaa gatgcaatga
     6181 tggtgaacgc gtggcgttat ctcgatccgc agggttcgcc gattgatttg attgaagcgt
     6241 cggacggttt ttacaccaaa acctatttgc aggtgtcggc cgaaggcgag gcgaaaccgt
     6301 taaacctgcc caacgattgc gatgtggtcg gctatctggc ggggcatctt ttgctgacgc
     6361 tgcgcaagga ctggaaccgc gcgaaccaaa gctatccgag cggtgcgctg gtggcggtga
     6421 agctgaatcg gggtgaactc ggggcggcgc agcttttgtt tgcgcctgat gaaacgcagg
     6481 cattggaaag cgtggaaacg accaagcgtt ttgtggtggc gagcctgctg gaaaacgtac
     6541 aaggccgtct gaaagcgtgg cgttttgccg acggcaaatg gcaggaagtc gaactgccgc
     6601 gcctgccttc gggcgcgttg gaaatgaccg accagccttg gggcggcgac gtggtttacc
     6661 ttgccgccgg cgatttcacc acaccgctga cgctgtttgc gctggatttg aacgtgatgg
     6721 aactgaccgt catgcgccgc cagccgcagc agttcgattc agacggcatc gaagtgcggc
     6781 agttttgggt gatgtcgtcc gacggcgaac gcatccctta tttccacgtc ggcaaaaacg
     6841 ccgcgcccga cacgccgacg ctggtttatg cctacggcgg tttcggcgta cccgagctgc
     6901 cgcattatct gggcagcatc ggcaaatact ggctggaaga gggcaacgcc ttcgtgttgg
     6961 cgaacatacg cggcggcggc gagttcggcc cgcgctggca tcaggcggcg cagggaatca
     7021 gcaaacacaa aagcgtcgat gatttgcttg ccgtcgtgcg cgatttgtcc gagcgcggca
     7081 tcagttcgcc ccaacacatc ggtttgcagg gcggcagcaa cggcggcctg attaccgccg
     7141 ccgccttcgt gcgcgaaccg caaagcatag gcgcactggt gtgcgaagtg ccgctgaccg
     7201 acatgatccg ttatccgctg ctctccgccg gttcaagctg gacagacgaa tacggcaatc
     7261 cgcaaaaata cgaagtctgc aaacgccggt tgggcgaatt gtcgccgtat cacaatcttt
     7321 cagacggcat cgattatccg cccgcgctca tcaccaccag cctcagcgac gaccgcgtcc
     7381 atcccgccca cgcgctcaag ttttacgcca aactgcgcga aacctcgccg caatcttggc
     7441 tctactcgcc tgacggcggc ggccataccg gcaacggcac gcagcgcgaa gccgccgacg
     7501 aactcgcctg cgtgttgctg tttttgaaag agtttttggg ctaagggcgg ggagcggcac
     7561 tgccgccgcg aatgaaaaaa ggtcgtctga aactgctttt tcagacgacc ttttttaatg
     7621 gttgtttcaa atcaaaatat ctatgccgcc ggccccatca gcacttcttc acatccgaag
     7681 gcaaaaatcc gtaatgccgt ctgaacgctt cgttgaaccg tcccgcgtgg cggtagccgc
     7741 aaaagtgcat ggcggcttgg acggtgctgc cggattcgat gagggcgagc gcgtgttcca
     7801 gccgcaggcg gcgcaggcat ccggcgacgg tttcgccggt ttgcgctttg aaatagcgtt
     7861 tcaggtagca ttcgttcagt ccgacgcggc gggcgatttc ggcgatggtc agcggacggg
     7921 cgaattcgtg ttgcaggatg tcggcggctt cgtctatgcg ccgacggcgg taaccgttgt
     7981 cgtggcggcg gaaggtgaag cgcaataatc gggcggagag ttccagcgcg gcggcttcgt
     8041 cggcaagcag gccgaagccg tccgattcga acgagcgttg cagcagtggg caggccgccg
     8101 ccgtcagtgc cgctgcgttt tgcgccagcc gttgcagggc gaatcggcct atcgtttgcg
     8161 gcgaaaacag gcgttcgtcc agcaagcctt cgtcgtgcca gcggcgcagt ttttccagcg
     8221 aaaaatccaa atgcagcgcg cacatgccgc tgttgtcggg cagcagggtt tcggatacgt
     8281 ccgccaactc gccgcgtacc agccagattt cgccggcaga cgggcggtat tccctgccgc
     8341 ccatttgtaa ccggttctgc cccgacacca tgacgaacaa ggcgcagttg tggctgaaat
     8401 tgtggatttc ggtgggaaac gcgcccgttc cgccgccgcg catccgcgac aaggtgatgc
     8461 ccgagtcgaa gcggttgatg cacatttcca gatgcaaacc gggctgtttt gcctgcgcaa
     8521 tgagcgcgct gtcggaacag ccgtccaacg cccagccgga tttatcggag cggacatagg
     8581 tttggtactg gcggtagatg gcggcggtgt tcatgattgg ataggaacga gttgtctaac
     8641 aaatgaatta aataggaatt attaccaata atcaagcgca gggattggtt gaaacggaaa
     8701 aggtcgtctg aaagggtgtt tcagacgacc ttttccgtat cgggaatttg ttttgccgta
     8761 tcgggaattt tgcgttttgc ggcgtggttt ctgcaggttg tttgcttaat aataaacatt
     8821 cttattcgta tgcaaaggaa ccgcacaccg tgaaaccgcg tttttattgg gcagcctgcg
     8881 ccgtcctgct gaccgcctgt tcgcccgaac ctgccgccga aaaaactgta tccgccgcat
     8941 ccgcatctgc cgccacactg accgtgccga ccgcgcgggg cgatgccgtt gtgccgaaga
     9001 atcccgaacg cgtcgccgtg tacgactggg cggcgttgga tacgctgacc gaattgggcg
     9061 tgaatgtggg cgcaaccacc gcgccggtgc gcgtggatta tttgcagcct gcatttgaca
     9121 aggcggcaac ggtggggacg ctgttcgagc ccgattacga agccctgcac cgctacaatc
     9181 ctcagcttgt cattaccggc gggccgggcg cggaagcata tgaacagttg gcgaaaaacg
     9241 cgaccaccat agatctgacg gtggacaacg gcaatatccg caccagcggc gaaaagcaga
     9301 tggagacctt ggcgcggatt ttcggcaagg aagcgcgcgc ggcggaattg aaggcgcaga
     9361 ttgacgcgct gttcgcccaa acgcgcgaag ccgccaaagg caaaggacgc gggctggtgc
     9421 tgtcggttac gggcaacaag gtgtccgcct tcggcacgca gtcgcggttg gcaagttgga
     9481 tacacggcga catcggccta ccgcctgtag acgaatcttt acgcaacgag gggcacgggc
     9541 agcctgtttc cttcgaatac atcaaagaga aaaaccccga ttggattttc atcatcgacc
     9601 gtaccgccgc catcgggcag gaagggccgg cggctgtcga agtattggat aacgcgctgg
     9661 tacgcggcac gaacgcttgg aagcgcaagc aaatcatcgt catgcctgcc gcgaactaca
     9721 ttgtcgcggg cggctcgcgg cagttgattc aggcggcgga gcagttgaag gaggcgtttg
     9781 aaaaggcaga acccgttgcg gcggggaaag agtagggagt cgtctgaaaa cggagtttcc
     9841 gaaggaagcg gggggtttct gcgaagctaa agtgcggttt caacgaattg aaaagcagcc
     9901 tgta
//
LOCUS       DAWKOG010000062         9847 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.062, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000062 DAWKOG010000000
VERSION     DAWKOG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9847
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.062"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(128..1174)
                     /gene="adhP"
                     /locus_tag="WJK66_001138"
     CDS             complement(128..1174)
                     /gene="adhP"
                     /locus_tag="WJK66_001138"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEZ7578859.1"
                     /translation="MKMQAVVVNKNVAGDVEIVEREVRPLKYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATHCIVSADYAVKVPEGLDPAQASSITCAGVTTYKAIKV
                     SGVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDDKLAFAKETGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(1445..1831)
                     /locus_tag="WJK66_001139"
     CDS             complement(1445..1831)
                     /locus_tag="WJK66_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ7578860.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLSEVK
                     STLLMNAQNLERYYRQKGTFDNYDQTKLKQNKYFKITLSKSPDHFTLKATPNPATNDG
                     ETCVVTLNDGGIIAASGTNQSCPGFD"
     gene            2057..3235
                     /locus_tag="WJK66_001140"
     CDS             2057..3235
                     /locus_tag="WJK66_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HEZ7578861.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQAAYITETVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKENATSKEDLESAQDAFAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            3301..5235
                     /locus_tag="WJK66_001141"
     CDS             3301..5235
                     /locus_tag="WJK66_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HEZ7578862.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARAEKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIRRLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIREKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAASVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            5249..5471
                     /locus_tag="WJK66_001142"
                     /pseudo
     CDS             5249..5471
                     /locus_tag="WJK66_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250036.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5554..6336)
                     /locus_tag="WJK66_001143"
     CDS             complement(5554..6336)
                     /locus_tag="WJK66_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEZ7578863.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAVKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAKGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            6475..8664
                     /locus_tag="WJK66_001144"
     CDS             6475..8664
                     /locus_tag="WJK66_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEZ7578864.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDTARILGVQTTFSDEPLLPESWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGEMTMAALKQVNAQA
                     AKLIEDWAEQGWIETTEAAKPVLRSCNGQASHSEFVLNADQQKASDAIQTALGRFQPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SRMAAGRRTQDYLRAMSGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPVVLGSATPSLESWHKAQSGAYRLLQLTERAHAAAQLPQVEIL
                     NAGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHRESIPFKCPDCGNQDLTAVGHGTQRVEETLRSFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADAMEFLNAAKETLAPLLPESVSQFGAAPMLMVRLA
                     ERERAQIFLESVSRQDLHRAVSLWVQVLQQNRDSKIRWSVDVDPQEA"
     gene            complement(8710..9784)
                     /locus_tag="WJK66_001145"
                     /pseudo
     CDS             complement(8710..9784)
                     /locus_tag="WJK66_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222887.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
BASE COUNT         2300 a         2544 c         2868 g         2135 t
ORIGIN      
        1 gacgtgttgc gatacgggtt ttcagacggt atttcaaaca gaacagatta aaatcaaatc
       61 caaatccata aaaaatgccg tctgaacagc gttcagacgg catttcgatg tgtactgcca
      121 cgtcaaatca gtggtgatga ccgcagccgc attctttttt catatcgatc accatacggc
      181 cggtgatttt gccttcgcgc atttcttgga aaatggcggg tgcttcatcc aaagcacgca
      241 gttggacttt cggaacaacc aaaccttccg cgccgaattg gaaggcttct tccaaatctt
      301 tgcgcgtgcc gaccaaagag ccgaccactt cgatgccgtc caaaaccaaa cgcgggatgg
      361 acaaatccat cgattccggc ggcagcccga tggcaaccac acgtccgccc gcgcggacgc
      421 aattcacggc agagttgaat gcggcagcag atacggcggt tacgaccgca gcgtgtgcgc
      481 cgccggtttt ttcctgaatc actttggcag cgtcctcttt ggcggcgttg acaaccaaat
      541 ccgcgccggt ttctttggca aacgccagtt tgtcgtcgtt gatgtcgatg gcgacaacgt
      601 gcgcgccgaa tacttttttc gcgtattgga cccccaagtt gcccaaaccg cccgcgccgt
      661 agatggcaat ccactgtccc ggacgaacgc cggaaacttt aatggcttta taagtggtca
      721 cgccggcaca agtaatgctc gaagcttgcg caggatccaa accttcaggg actttgaccg
      781 cgtaatcggc actcacgatg cagtgggtcg ccataccgcc gtcggcggtg tagcccgcgt
      841 tcaatacgga acggcacagg gtttcgcggc cggtattgca gtattcgcac gagccgcagc
      901 tttggaacag ccaggcgatg ctgacgcggt cgccgacttt cagatttttc acaccgtcgg
      961 caacttcttt aaccaaaccg atgccctcgt gtcccagcac gcggcccggt ttttcgccgt
     1021 agtcgcctgc cgcaacgtgc aggtcggtgt ggcacacgcc gcaatattcg acttcgacca
     1081 acgcctcgcc gtatttcaac gggcgaacct cgcgttcgac tatttccaca tcgcccgcta
     1141 catttttatt cacaacaact gcctgcattt tcatgattgc gctccttagt tacggcaaaa
     1201 aaacctgtaa acggaatgtt gtccgatata agggtaagca tacgcttccg catcttacag
     1261 gtcaagtggt atgttgttga aaaatatatg ttatattata acagctcgga aaggcgcggc
     1321 atcggggcgg ttgccggggc ggcaggtttc aagtttgaaa aaccggatgg gcaaacccgt
     1381 aaagataccg tctgaagctg tgtccggacg gcatctttac gggtttgcgg gcttcggcgg
     1441 aggattagtc gaagccgggg caggattggt ttgtaccgga agcggcaatg ataccgccgt
     1501 cgttgagtgt aacgacgcag gtttcaccgt cgttggtggc gggattgggc gttgccttaa
     1561 gggtgaagtg gtcggggctt ttgcttaagg taatcttgaa atatttgttt tgtttcagtt
     1621 tggtttggtc gtagttgtca aacgtccctt tttggcggta gtaacgctcc aagttttgcg
     1681 cgttcatcag cagggtggat ttgacttccg acaggcggac gcgccggatg taggttttat
     1741 aggaagggta ggtgatgagc gtcaggatgc cgaggatggc gacggcaatc atcagctcga
     1801 gcagcgtaaa gcctttttga acgtttttca tagcaatgtg tttccatttg tttgtcggtc
     1861 ggcgacatta taacgccgta tcggaaatgg cggaatatgt aaacggattg aaattttcgg
     1921 gaaagcagat tgtataagcc atttaaaaca aatggttatt tttattgtcg gcagtttgcc
     1981 gccttggatg gggcagggac ttgcggtaga atccgcttcc gatttatggg attgacgcat
     2041 acagagaatt gaaaacatgg caaaaatgat gaaatgggcg gctgttgcgg cggtcgcggc
     2101 ggcagcggtt tggggcggat ggtcttatct gaagcccgag ccgcaggctg cttatattac
     2161 ggaaacggtc aggcgcggcg acatcagccg gacggtttct gcaacagggg agatttcgcc
     2221 gtccaacctg gtatcggtcg gcgcgcaggc atcggggcag attaagaaac tttatgtcaa
     2281 actcgggcaa caggttaaaa agggcgattt gattgcggaa atcaattcga cctcgcagac
     2341 caatacgctc aatacggaaa aatccaaatt ggaaacgtat caggcgaagc tggtgtcggc
     2401 acagattgca ttgggcagcg cggagaagaa atataagcgt caggcggcgt tatggaagga
     2461 aaacgcgact tccaaagagg atttggaaag cgcgcaggat gcgtttgccg ccgccaaagc
     2521 caatgttgcc gagctgaagg ctttaatcag acagagcaaa atttccatca ataccgccga
     2581 gtcggaattg ggctacacgc gcattaccgc aacgatggac ggcacggtgg tggcgattct
     2641 cgtggaagag gggcagactg tgaacgcggc gcagtctacg ccgacgattg tccaattggc
     2701 gaatctggat atgatgttga acaaaatgca gattgccgag ggcgatatta ccaaggtgaa
     2761 ggcggggcag gatatttcgt ttacgatttt gtccgaaccg gatacgccga ttaaggcgaa
     2821 gctcgacagc gtcgaccccg ggctgaccac gatgtcgtcg ggcggttaca acagcagtac
     2881 ggatacggct tccaatgcgg tctactatta tgcccgttcg tttgtgccga atccggacgg
     2941 caaactcgcc acggggatga cgacgcagaa tacggttgaa atcgacggcg tgaaaaatgt
     3001 gctgattatt ccgtcgctga ccgtgaaaaa tcgcggcggc aaggcgtttg tgcgcgtgtt
     3061 gggtgcggac ggcaaggcgg cggaacgcga aatccggacc ggtatgagag acagtatgaa
     3121 taccgaagta aaaagcgggt tgaaagaggg ggacaaagtg gtcatctccg aaataaccgc
     3181 cgccgagcaa caggaaagcg gcgaacgcgc cctaggcggc ccgccgcgcc gataaacgaa
     3241 tatgccgtct gaacacggaa acggtttcag acggcatttg ttattgattt acggaatatt
     3301 atgagcttga tcgaatgtaa aaacatcaac cgctatttcg gcagcggcga gaaccgcgtc
     3361 catattttga aagacatcag cctgtcgata gagaagggcg attttgtcgc catcatcggg
     3421 cagtccggtt cgggaaagtc cacgctgatg aacatcctcg gctgtttgga taccgccggt
     3481 tccggttcgt accgaatcga cggcatcgaa actgccaaaa tgcagcctga cgagctggcg
     3541 gcattgcgcc gcgaacgctt cggtttcatc ttccaacgct acaacctctt aagctcgctg
     3601 accgcaaggg ataacgtcgc gctgccagcc gtctatatgg gcgcgggcgg caaagagcgt
     3661 tccgcgcggg cggagaagtt gttacaggat ttgggtttgg caagcaaaga gggcaacaag
     3721 cccggcgaac tctcgggcgg acagcagcag cgcgtctcca tcgcccgcgc cctgatgaac
     3781 ggcggagaaa tcatcttcgc cgacgagccg accggcgcac tcgataccgc cagcggcaaa
     3841 aacgtgatgg aaatcatccg caggctgcac gaagccgggc ataccgtcat tatggtcacg
     3901 cacgaccccg gcatcgccgc caatgccaac cgcgtcatcg aaatccggga cggcgaaatc
     3961 atttccgaca cctcgaaaaa tcccgaaatc cccgcaagca atgtcgggag gattcgggaa
     4021 aaagcctcgt ggtcgtttta ttacgaccag tttgtcgaag ccttcagaat gtcggtgcaa
     4081 gcagtattgg cgcacaaaat gcgttcgctt ctgacgatgc tcggcatcat catcggtatc
     4141 gcttcggttg tctccgtcgt cgcattgggc aatggttcgc agaaaaaaat ccttgaagac
     4201 atcagttcga tagggacgaa caccatcagc atcttcccgg ggcgcggctt cggcgacagg
     4261 cgcagcggca ggattaaaac cctgaccata gacgacgcaa aaatcatcgc caaacaaagc
     4321 tacgttgctt ccgccacgcc catgacttcg agcggcggca cgctgaccta ccgcaatacc
     4381 gacctgaccg cttctttgta cggcgtgggc gaacaatatt tcgacgtgcg cggactgaag
     4441 ctggaaacgg ggcggctgtt tgacgaaaac gatgtgaaag aagacgcgca ggtcgtcgtc
     4501 atcgaccaaa atgtcaaaga caaactcttt gcggactcgg atccgttggg taaaaccatt
     4561 ttgttcagga aacgcccctt gaccgtcatc ggcgtgatga aaaaagacga aaacgctttc
     4621 ggcaattccg acgtgctgat gctttggtcg ccctatacga cggtgatgca ccaaatcaca
     4681 ggcgagagcc acaccaactc catcaccgtc aaaatcaaag acaatgccaa tacccaggtt
     4741 gccgaaaaag ggctgaccga tctgctcaaa gcgcggcacg gcacggaaga tttcttcatg
     4801 aacaacagcg acagcatcag gcagatagtc gaaagcacca ccggtacgat gaagctgctg
     4861 atttcctcca tcgccctgat ttcattggta gtcggcggca tcggcgtgat gaacatcatg
     4921 ctggtgtccg ttaccgagcg caccaaagaa atcggcatac ggatggcaat cggcgcgcgg
     4981 cgcggcaata ttttgcagca gtttttgatt gaggcggtgt taatctgcgt catcggcggt
     5041 ttggtcggcg tgggtttgtc cgccgccgtc agcctcgtgt tcaatcattt tgtaaccgac
     5101 ttcccgatgg acatttcggc ggcatccgtt atcggggcgg tcgcctgttc gaccggaatc
     5161 ggcatcgcgt tcggctttat gcctgccaat aaagcagcca aactcaatcc gatagacgca
     5221 ttggcgcagg attgaggttg gaacaaagat gccgtctgaa gctgcaggac cggtcatttt
     5281 ggagcagaaa cttattggac aaaaacggtt tcttagattc tacgttccag attcccactt
     5341 gcgtgggaat gacggcggtg tgtttcgtat tgttcagttt attattttca atcattcaat
     5401 gggttaggat gtgtttgttg gcttgctgta ctggtttaaa tttaatccac tataaattta
     5461 atccactata actttcagtg cggattggtt ttcagacggc atttcccgca aaaaaggctt
     5521 ggaattttcc aagccttttg ttttgcggat ggattattga tttttgcgga tgatttcctc
     5581 cagttggggc atcgggctgt agccgctttg gctgcgcccg ttggggaaga cgagggtcgg
     5641 cgtgccgttg aagccgaatt gttcgcccaa ggaagtggtt tccgcgacgg gattgtcgca
     5701 gatgctgccg ccgaccggga atttgccttt acgcatccaa tccgtccacg ctttggcgcg
     5761 gtcgggctga caccataaga tttgcgcctt gcgcgcggca tcggggtgca ggccggcaat
     5821 gggcatcata aagctgtaaa ccgtcacgtc ggtcattttt tcaaactcgt gttccaagcg
     5881 tttgcagaac ggacaatcgg ggtcggagaa gacggcgact ttcagcttgc cgttgccgcg
     5941 cacttctttg atggctttgt ccaaaggcag ggaggcgaag tcgattttgt tcaaatcggc
     6001 ggcgcgttct tcggtcaggt ttttgcgcgt gtcgatgttg atgagttcgc cgacgaacat
     6061 atagccgcct ttggcatcgg tgtagataat ctgcctgccg ctgacgacga cttcgtaaat
     6121 gcctttgacc ggtgtttcgc tgacgctcaa cactttcaaa tcttgggcgg aataggtttt
     6181 ttccaaacgc gctttcaaag aggcggcaac ggatttgccg gcggactcgg ctttgacggc
     6241 gggttcggcg ttggcattgg aaacgggcgt ttgcccgcaa gccagcagtg ggaggacggt
     6301 aaagggggtc aagattttga ttaacttggt tttcatataa agatgattgc gcgtgttgga
     6361 aaagcggaat tgtatcaaat ctctgttgcg cctgcattgc gcctaggctc aatttatcgt
     6421 ctgaaaatag cttccgcctg ttaaaatacg caaaaaaatg atttgcctgc ctgtatgatc
     6481 taccaccgca tcgccgtaaa cgtgccgctt tcagacggcc ttttgactta ttcccattcc
     6541 gatccgcttc ctccgggaac gcgggtgctt gtgcctttcc gcaataaaac cgtggtcggg
     6601 atggtgtggg aaacggatat tgcgccggat atggatacgg cgcggatttt gggcgttcag
     6661 acgacctttt cggacgaacc gctgctgccc gaaagctggc gtgatttgtt ggcgtttacg
     6721 tcgcgttatt accactatcc gaccgggcag gcagtatttg ccgcattgcc gcaaggtttg
     6781 aaggaaacgc gcgcggtgga aatgccgcag ccgcccttgt tttacgcgtt gaacgaagcg
     6841 ggcagggcgc aaacgccgcc gccggcgcgg ttcaacaaaa aagcggcttt gtgggacgcg
     6901 ctgctgtcgg gcgaaatgac gatggcagcg ttgaagcagg taaacgcgca ggcggcgaag
     6961 ttaatcgaag attgggcgga gcagggttgg attgaaacga cggaagcggc gaagcccgtt
     7021 ttaaggtcgt gtaatgggca ggcttcgcac tctgaatttg tattaaatgc tgaccagcag
     7081 aaggcttccg atgccattca gacggcattg ggacgcttcc agccgtttct gctgtacggc
     7141 atcaccggca gcggcaagac cgaagtgtat ttcgatgcga tggcgaaagt gttggctcag
     7201 gggcggcagg tgttgtttct gttgcccgaa atcaacctca cgccgcagct tttgaagcgg
     7261 gtggaaaacc gttttgccga cgtgccgacc gccgtgttgc acagccggat ggcggcaggc
     7321 aggcgaacgc aggattatct gcgcgcgatg tcggggcagg cgaagctggt gatcggcacg
     7381 cggctggcgg tgttcacgcc gatggatgat gtcgggctga ttgtggtcga tgaggaacac
     7441 gacggctcgt tcaaacagga caacgaattg cgctaccacg cccgcgattt ggcggtgtgg
     7501 cgggcgaagc agggcggctg tcccgtcgtg ttgggcagcg cgaccccaag cttggaaagc
     7561 tggcacaagg cacaaagcgg cgcgtaccgc ctcttgcaac tgaccgaacg cgcccatgcc
     7621 gccgcgcaac tgccgcaagt agaaatcctc aacgcaggcc gtctgaaact cgacaacggt
     7681 ttctcgccgc aagccttgca gcttctgaaa cagaattttg aagcaggcgg tatgtcgctg
     7741 gtgtacctca accggcgcgg ctttgcgccc gcgctgtttt gcggcgactg cggccatacc
     7801 ttcggctgcc cgaactgctc cgccaaaatg gtgttgcacc aacgcgcccg ccaactgcgc
     7861 tgccaccact gcgaccaccg cgaatccatc ccgttcaaat gccccgactg cggcaaccaa
     7921 gacctgaccg ccgtcggcca cggcacgcag cgcgtcgaag aaaccctgcg ctccttcctg
     7981 cccaaggccg ccgtcgtccg tgttgacagg gacagcacgg cgcacaaaaa cgactgggcg
     8041 gatttgtacc gccgcattgc cgacaacgaa atcgacattc tggtcggcac gcagatgctc
     8101 gccaaaggcc atgatttcgc gcggctcaac ctcgttatcg tgttgaacgc cgacggcagc
     8161 ctgtacagcg cggacttccg cgcaccggaa aggttgtttg ccgagttgat gcaagtgtcc
     8221 ggcagggcgg ggcgcgccga caaacccggc aaggtgctga tacagaccca actgcccgaa
     8281 catcccgtct tcgccgccgt caaagcgcag gattatgccg tgtttgccga aaatgaattg
     8341 aacgagcggc aaatgttcgc gatgccgccc ttcggtttcc agaccgccgt ccgcgccgat
     8401 gcgccgcgtg ttgccgatgc gatggaattt ctcaatgccg ccaaagaaac cctcgccccg
     8461 ctgttgcccg aaagcgtttc ccagtttggc gccgccccta tgctgatggt gcgccttgct
     8521 gaacgcgaac gcgcgcaaat cttcctcgaa tcggtatccc gacaggattt gcaccgcgcc
     8581 gtgagtttgt gggtgcaggt gttgcagcaa aaccgcgaca gcaaaatcag atggtcggtg
     8641 gatgtcgatc cgcaggaggc ttgattatcg gcaatacgat gccgtctgaa accgtttcag
     8701 acggcatttt tattccgggt cgtccgtaaa cgcattcgcc cgaaaaatcg gaacaaacgt
     8761 gaaaagataa agcgtgaaca cgacggcggt cagaatcgcg gggacggtga tgaagaatat
     8821 tggattcacg ttcatcaaga aagcacgcga gacggcagcc ataaagagca ggaggacggc
     8881 gatgaggcag agtttcgggt agtcgagctt ggtaaagccg ctgtgccaca gtccggcggt
     8941 cagccacacc atcatcacgc cgcccatcat gtcgccgagg gtaatcaggt gcaggggcgc
     9001 ggaggcgggc aggttttgca gtttcgccgc acctgtccac aaatagcctg cggcggcaaa
     9061 aagctggagc aggtaataag tgcggacgta gtgtttgcgc aggagttcgt gatggtgcag
     9121 ttcgcgcagc ttggcgagca ggatgaaacc gacggcaagc gcggtaaaac cggcggtttg
     9181 cgcaggcagc caaagttcgg cggcggcgtg cagcagcagg aaggtgatgg cgatgttttt
     9241 atagacaacg ttggggatga atacggggtc tttcagacgg cattctttca gggcttccgc
     9301 gcccaaaaga atactgacgc gcacggatac gaacatcacc gccgccatat ttagatgcac
     9361 ttgcgcgcgc aacaggttca aatcgccgct gacggcatat gccgtctgaa aaacagtgaa
     9421 cgcggcaagt aacattagca gggcgaagtt gtcggtgttt cggtctagcc aaatcagccg
     9481 ggcgcagaac agcagcaaca ccagccaata ggcggcgacg aaaaacgagg cagtttgcgg
     9541 cgaaaagggc agtatagcgg atgcggcgag caataatgcc gccatcaaag tcgcgacagg
     9601 tttcaggtta cccgaaaaaa accgtccagt ccaacaaagc cgcagtcagg aaaccaccat
     9661 atgccgcagg tagcatgagt tctaagaaga tttggcggtg caggacgacg gcaccggggc
     9721 tgatgaaaaa caccaacgcg ccgagtatgg caagcactgt cgcgccgacg aaaaacgggc
     9781 gcatagcaac tgtatttttc accccgtcgg gcaaaaatac caaaactcaa atcaagccgt
     9841 ttagata
//
LOCUS       DAWKOG010000063         9794 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.063, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000063 DAWKOG010000000
VERSION     DAWKOG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9794
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.063"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(10..1305)
                     /locus_tag="WJK66_001146"
     CDS             complement(10..1305)
                     /locus_tag="WJK66_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ7578865.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMEEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAIRSQHWEELQQWHEHAQKYYGATHYQL
                     WDNTELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGIARAAAEAGTKIFEHSPMIRIEP
                     YQNGWLVYTPEGSVECKNLVYAVNTYAGLNPIFKPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDHEFIDDPARMTELVRQDMLKVFPQL
                     DDVKIEYSWGGECDITANLVPHFGRLAANVFYAQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1431..2318)
                     /locus_tag="WJK66_001147"
     CDS             complement(1431..2318)
                     /locus_tag="WJK66_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ7578866.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGMLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAN"
     gene            complement(2318..3283)
                     /locus_tag="WJK66_001148"
     CDS             complement(2318..3283)
                     /locus_tag="WJK66_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ7578867.1"
                     /translation="MNLNKLKNKLFRRPGQRAVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTSIDEDLGRLNIAISYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALMTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3299..4423)
                     /gene="potA"
                     /locus_tag="WJK66_001149"
     CDS             complement(3299..4423)
                     /gene="potA"
                     /locus_tag="WJK66_001149"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HEZ7578868.1"
                     /translation="MTATTASSTKPYLQIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLGNG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4618..4725)
                     /locus_tag="WJK66_001150"
                     /pseudo
     CDS             complement(4618..4725)
                     /locus_tag="WJK66_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4804..5073)
                     /gene="rpsO"
                     /locus_tag="WJK66_001151"
     CDS             complement(4804..5073)
                     /gene="rpsO"
                     /locus_tag="WJK66_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEZ7578869.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5296..6231)
                     /gene="secF"
                     /locus_tag="WJK66_001152"
     CDS             complement(5296..6231)
                     /gene="secF"
                     /locus_tag="WJK66_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEZ7578870.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(6235..8088)
                     /gene="secD"
                     /locus_tag="WJK66_001153"
     CDS             complement(6235..8088)
                     /gene="secD"
                     /locus_tag="WJK66_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEZ7578871.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQFKVDAALKNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGAVWKPEAETAAGKE"
     gene            complement(8300..8566)
                     /gene="yajC"
                     /locus_tag="WJK66_001154"
     CDS             complement(8300..8566)
                     /gene="yajC"
                     /locus_tag="WJK66_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEZ7578872.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAISAKVD"
     gene            8694..>9794
                     /locus_tag="WJK66_001155"
     CDS             8694..>9794
                     /locus_tag="WJK66_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEZ7578873.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACAGHEPGANHPDS
                     PDRILCIEQALRRASIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCLPQKGKISRL
                     DNDTAISTGSLSAARFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRY"
BASE COUNT         2516 a         2583 c         2424 g         2271 t
ORIGIN      
        1 ctgcgacgct taacggctgt ctttccactg ataatatttc gagccgagga aagaaccgag
       61 tttgcgcaaa aacggttgca ggataatatt cggctggcgc aaccgctcaa acggcttcag
      121 acggcattcg tcccctaaaa ttgcttcggc aaccgccaga cctgcaatgc ctgttatcgc
      181 catcccgtgt ccggaataac cttgcgcata aaaaacattc gcggccaaac gtccgaaatg
      241 cgggacaagg ttggcggtaa tgtcgcactc cccgccccac gaatattcga ttttgacatc
      301 gtcaagctgc ggaaaaactt taagcatatc ttggcggaca agttcggtca tacgcgcggg
      361 atcgtcgata aattcatggt ctttaccgcc gaaaagcagt ctgccgtccg cgctgaggcg
      421 gtaataatcc aaaatatggc ggttgtcgca tactgccata ttgttacgga taagcccttt
      481 tgcgcgcgcc cccaagggtt cggtcgcaat aataaaggtg ctaacggcaa tcgccttgcg
      541 ctccaaaggc ttgaatatcg gattcaaacc ggcataagta ttgacggcgt acaccagatt
      601 tttgcattcg acgctgcctt cgggggtgta aaccagccaa ccgttttgat aaggttcaat
      661 gcgtatcatg ggagaatgct caaaaatctt cgtaccagct tcggcagcgg cgcgggcgat
      721 gcccaacgtg taattgagcg gatggagatg cccggacaag ggatcgaact gtgcgccttg
      781 gtacatatcg ctgtcaagct gctgcttcag ttcagtgtta tcccagagtt gataatgagt
      841 tgcaccgtaa tatttttggg cgtgctcatg ccattgttgc aattcttccc aatgctgcga
      901 acggatggca accgtggcat aaccgcgctg ccaatcgcaa tcaatggcat gtttgcggac
      961 gcgttcgtcc accagttcga ccgcctgcaa agactgttgc caaaaccatt gcgcttgctc
     1021 caaaccgacc tgtttttcaa tttcctccat accgcaggcg taatcgctga taacctgccc
     1081 gccactccgt cccgacgcgc cgaaaccgat acgcgcggct tccaacacaa ccgtttcatg
     1141 tccctgctcc gccaagggca atgcagtgca caaaccactc aatccgccgc cgatgataca
     1201 ggtatcggtt ttcagacggc attgaagttt cggataaaca gtatgaggat taaccgaact
     1261 gaaataataa gaaggcagat attcttgaaa atcagggcga atcattgtgt ttgctttatc
     1321 gggtatattt tcggacggaa tgatacaggc tgtcgagcca tatcgtccaa acagaaaatc
     1381 ggttgaagaa aacaggctga cccagtcatg cggtcagcct gccttattaa ttaatttgct
     1441 ttctcggcag ccaatttttc ttggcggtag gcttctgccg cttctcggtc acgcttggtt
     1501 gcctgcctca tcatccaata attgacgatg atgaccaatg ttccgatgat gccgatcagg
     1561 atggttgcca aaacgttcat ctgcggatca agacccagct tgattttgga gaaaatcacc
     1621 tgcggcaatg tggatgaacc ggggccggag aggaatgagg taatcaccaa atcatccaaa
     1681 gacagggtaa tgccgagcag aaagcctgaa gcgatggcag gggcaatcaa aggcaaagtg
     1741 atgacaaaaa agattttcag cggacgcgcg cccaaatcca ttgcggcttc ttcgagcgat
     1801 tggtcaagct caaccagacg cgaacggata acaacggtaa tgtatgccat gcacagcgtc
     1861 gtatgtccta agaaaatagt gaaaaagcca cgatcgaagt agagatgttg taaccattcg
     1921 ctgccctgca aaaatatctg tacctgaata atcagcagca gcatagacag accggtaatc
     1981 acgtcgggca tcaccatagg tgcggaaatc ataccggcga acaaggtact gccgcgaaaa
     2041 cgtttaatcc gcgccatcgc atagcccgcc agcatgccca aaacgacggc ggcaagcgaa
     2101 gacacaacgg caatccgcag cgacagccaa gcggcttcca aaatggtgtc gttttccaac
     2161 aatgcgccgt accacttggt cgaaaagccg ccccaaacgg ttaccagctt ggattcgtta
     2221 aacgaataga tgaccaaaac aaccagcggg atatacagaa acgccagcga cagtgccaac
     2281 atcagtttca agaaccaaga taatttggat ttctgcatta tttggctcct tcttccaatt
     2341 cgcggttttc ataatgctga aacagggcaa tcggcacgac cagcagcgcg accatcacga
     2401 cggcgacggc ggaagccagc ggccagttgt tttgatcgaa gaacgcctgc cacaagactt
     2461 taccaatcat caggttttcc gaaccgccga ccagctcggg aatgacgaac tcgccgacag
     2521 cagggacgaa aaccagcatg gagcctgcaa taatgccggt tttcgacaaa ggcagggtaa
     2581 tcgtcaagaa cgatttgacc ggccccgcgc ccaaatcgga agccgcttca agcaggcggt
     2641 tgtcgagttt caccagttgc gtgtatagcg gcagaatcat aaacggcaga taggcgtaaa
     2701 ccatcaccaa attgagcgaa aaggcattgt agaacaaatc caaaggctcg ctgataatac
     2761 ccattttaat caacaggttg tttacaatgc cgttatgccc gagcagaccc atccacgcat
     2821 agacgcgcaa caggaacgat gtccaaaagg gcagcataat ggcaagcagc aaaccattgc
     2881 ggacagaagg attggcacga gaaatcgcat aggcggtcgg ataaccgacc aacaggcaaa
     2941 tcactgtcgt catcaatgcg gttttgattg aagaccagta ggtcatcaga tagatattgc
     3001 tgttttcact gtcgccgaac gggttaaggg tatgccaaaa attttggaaa atatcggcat
     3061 agttttgata actgatggcg atattcagac gacctaaatc ttcgtcgatg ctggtcagcg
     3121 gcgtaaacgg cggaatggcg atttcttgtt cggcaaagct gattttcagc acgatggcga
     3181 acggaatcag aaacagcacc aaaagccaaa tatacggtac ggcaatcacc gcacgctgcc
     3241 ccggacggcg gaacagtttg tttttcagtt tattaaggtt cattgcattc cccttaaatc
     3301 aacggaacaa cggagtcggt tggttttccg gccagctgat atagacggtt tcgtcccaag
     3361 tcggcggtgt aatgttgcgc acataccagt aaggggcggg gacttggctt ttgacgacgc
     3421 gcccgttgcc gagcttgata tggtaaatgg cgaagctgcc caaataggcg atttctttta
     3481 ccgtgccttt cgcccagttg tagtcgccca aatattcggg tttttcttta tataaatcaa
     3541 tatcctctgg tcgaatacta acccaaaggt cctgctcgct cggaccaccc aaaccgtgat
     3601 cgatgcggac gtggttttcc aaaccttcgc attcgataac ggcatagtcg gcatgatctt
     3661 caatcaccac accgtcaaag atgttggttt cgccgataaa ctcggcagtg aagcggctgt
     3721 tgggatagtc gtacacgtcg ctgggtgtgc cgacttgctg caactgaccg tcagacataa
     3781 tggcgatgcg ggtcgccatc gtcatcgcct cttcttggtc gtgcgtaacc ataatacagg
     3841 ttacgccgac ttgttccagc gtattgacca actcaagctg ggtttgttgg cgcagttttt
     3901 tgtccaatgc accgaggggc tcatccagca gtagaatttt cggacgtttt gccagactgc
     3961 gtgccaaagc aatgcgctgc tgctgaccgc cggacaattg gtgcggtttg cgtttagcaa
     4021 atttggtcat ctgaaccagg cggagcattt cttcgacgcg cgcggcgatt tcgcctttag
     4081 gcattttgtc ctgtttcaga ccgaaggcaa tgttttgttc tacggtcata tgcggaaaaa
     4141 gcgcgtaact ttggaacatc atattgatgg ggcgatcata gggtgcaagt ttggtaatat
     4201 cctgaccatc aaggataatt tttccctgat tgggactttc catacccgcc agcatacgca
     4261 gcagtgtaga ttttccgctg ccggaactgc ccaaaagggc gaagatttcg tgttgataaa
     4321 tgtccaagtc gatgttatcg acagcgtaat tgtcaccaaa ctttttcacc aaaccttgga
     4381 tttgcagata aggcttggtt gaagacgcag tggttgcggt cataatggca atactccaat
     4441 aaaaagacga gtaccggcaa aacggatgtt cgaatgggtg ataaaaagct gtttgattgc
     4501 tggcgggagt taaacgtttg atgccgtctg aaactcttgt aaagcgcacg ggcagcatga
     4561 aatggaacaa gattccaaag aactttatat tatattagtt tatgcggttt tcgggcaata
     4621 tagtggatta aatttaaacc agtacagcgt tgccttgcct tgccgtacta tttgtactgt
     4681 ctgcggcttc gtcgccttgt cctgatttaa atttaatcca ctataaatat ggatttgagc
     4741 ttgtcggaaa gcaacagaaa agaaaacacc gcccattttt ctgggcggtg tcggaaagcg
     4801 taattattta cgcagaccca agcgggtaat caacgcgcga tacgtatcgg gctgggtacg
     4861 gcgcaagtag gccagcaggc ggcggcgttg gctgaccatt ttcaacaggc cgcgacggct
     4921 gtggtggtct tttgggttgg ctttgaagtg gggggtcagg tcgttgatgc ggaaagtcaa
     4981 cagagcgact tgtacttcgg aagagccggt gtcgccttct ttgcgttgga aatctttaac
     5041 gatttgtgct ttttgttcta cggtcagtgc cataatgaaa actccaaaaa tataagaatc
     5101 cccataggga ttcagacaag tttgccaagc ctgaagacaa cggcaaactc cctatgccca
     5161 agataggaca acgtggcatt atgacacaat tcccgcattt ctgcacaata ttttaagacc
     5221 gtggaagtaa ccgttaaaaa tgccgtctga agcattgtct gcttgagacg gcaatgttca
     5281 gacggcatat gcgcttcaaa ccacgatttc ttctttcttc ttcggttctt taccgatatt
     5341 gtcgcggctc aaaccgaaca ttagcagaag cgggctggca accaatacgg aagaataaat
     5401 gccgaacacg atgccaatgg tcaacgccat agaaaagccg tgcaaggccg caccgccgaa
     5461 caccagcatg gatacgacca tcgcctcggt cgaaccgtgg gtaatgatgg tgcggctcat
     5521 cgttgcggta atcgcgttgt cgatgacttc cggcacggca tgtccgcgca tcgccggctt
     5581 gcggaagttt tcacggatac ggtcgaagac gacgacggat tcgttcacag aatagcccaa
     5641 tacggcaagg atacccgcca agacggtcag cgaaaattcc cattggaaga aggcaaagca
     5701 gccgagaata atcacgatgt cgtgcatatt ggcgataatg gcagatacgg caaaacgcca
     5761 ttcaaaacgc atcgacaggt aaataatgat gccgataacg acaaaaccta aagccatcaa
     5821 tccattactt accaattcct caccgacttg cgggccgata aattcgactt ggcgcaaggt
     5881 aacgtcggga ctgtcttttt tcagcaaatc cataacctga ttggacaact gtgcggaagt
     5941 aacaccttct ttgttcggca ggcggatcat gatgtgtttg ttcgtaccca atgcctgaac
     6001 ctgtacatca cctattttca gcgtatcgag gcgttcgcgc atcttattga catccgcacc
     6061 ctgctgatat tggacttcca ttaccgtacc gccggtaaat tcgacagaga aattcagacc
     6121 tctggtaacc aaaaagaata cggcggcaat aaaggtaacc aacgaaataa aggtcgtcag
     6181 tttgccgtag ctcataaacg gaatatcgcg tttgatttta aagagttcca tagcttactc
     6241 cttacctgcc gcagtttcgg cttcaggttt ccataccgca ccgatggaaa tattctgcaa
     6301 tttgcgtctg cgtccgtacc acagattgac caacgcacgg aatacgacga cggaggaata
     6361 catcgaagtc agaataccca aacagtgtac gaccgcaaaa ccgcgtaccg ggccggaacc
     6421 gaataccaaa agcgcgatac cggcaatcag cgaagtcagg ttggaatcga caatggtcgc
     6481 ccatgcgtgt tggaaaccga gattgattgc ctgctgcggc ggcacgccgg cacgcaattc
     6541 ttcgcggata cgttcgttaa tcaagacgtt ggagtcgatt gccataccca aagtcaacgc
     6601 cagcgcggcc atacccggta acgtcaacgt tgcctgcatg gcagacaaaa taccgattag
     6661 gaacagtatg ttggcactca atgcaatggt agaaaagaaa cccatcagac gatagtaaac
     6721 caccatgaat gcagcaacga tggcaaaacc ccataaagtc gaatggaagc ctttttcgat
     6781 gttctcctta cccaaagacg gaccgatggt acgttcttcg acaatctgca tcggcgcggc
     6841 aagagaaccg gcacgcaaca gcaaagacgt atcattggct tcggctgtcg tcatgcttcc
     6901 ggaaatttcc acgcgtccgc ctgtaatggc agtacggata accggcgcgg ttacaacctc
     6961 ggattttcct tggtcgatca aaaccatcgc catgcgtttg ccgacatttg cggcagtcag
     7021 ttcgccgaaa atgctgccgc ccgcgctgtc caagctcaga ctgacggcag gtgcgcccat
     7081 ttggtcgaaa ctcggttgcg catcgttgat gttgtcgccc gtcagctcga cctgtttgct
     7141 gattagcaga gtttcgggac gatctccgcc gcttgaaagc agctcataac cgctcggcac
     7201 gttgccttcc aatgcctcgc gcaacttggc aggatcgtcc tccaccatac gcaattccaa
     7261 agtcgcggta cggccgatga tgtcttttgc cttggcagta tcctgaacgc ccggaagctg
     7321 cacgacgata cggtctgcac ctgactgctg gatgacgggt tcggccacgc ccaactcgtt
     7381 cacacggttg tgcagggtag tgatgttctg tttgaccgca tcggaacgca ctttattgac
     7441 cgcctcttcc gaaagcgtca agacgatatt gctgccgtct gaattcagcg ttgcttcagg
     7501 aaacagcttg cgcaactgcg gcagtgcctt ttgcacatca cctgcatcct gcaaagggac
     7561 ggtcaggctg tttccagcct gacgcaccgt gccgctgcgg attttttcgc ggcgcagttc
     7621 gcggcggatg tcgcccgaat aacgttcaaa cgttttctgc atcgccgctt tcatatcgac
     7681 ctgcatggtg aaatgcacgc cgccgcgcaa gtccaaaccc aaaaacatcg gattggcttt
     7741 gattttcgcc atccattcgg ggctgtccgc caacaggttg agcgcggtaa tatacccttc
     7801 gcccaaagtg ttttcgatga cgtcgcgcgc tttaagctgc gtttctgtgt ctttgaaacg
     7861 cactttcagt gaattgtcca caacaaacat cccgtcggtc tgaatacctg cgtttttcag
     7921 cgcggcatcc actttgaatt gagtctgttc gttgatgatg atggcttgtc ggttggtcga
     7981 tacctgcacg gcgggtgttt cgccgaatag gttgggcagc gaatacactg cggcaaccgc
     8041 aatcgtgaac acaatcagca gatatttcca taaaggataa cggttcatca ttgttcctta
     8101 atggttggaa ccccaccctt tcggtggtgt cggaatcggg ctatttcaga agaggcaaaa
     8161 acccttccca gccaggcaag accggaaagc ggcatcctga atatgccgcc ctgcgtgtcg
     8221 gaacatggtc aagccttcgg ttggaattca aaacaaagtg ccgcattcgg gcttttccga
     8281 tgcggcttgt cggcacaaat caatcgactt ttgcagaaat cgcattgcgt tccacttcga
     8341 cctcgatttt tgtaccctgt ccgatatcca cggtaaaaaa ctgttcgccg actctggtta
     8401 ccttaccctt gaaacctgcc gccaagacca ctttgtcgcc gactttcaag gcggcaagca
     8461 ttgcctgatg cgctttgaat ttcttttgct gcggacgcat gatcaggaag tagaacacca
     8521 ccataatcaa cactaaagga gcaaattgtg caacagcttg attcataatt tatccgttct
     8581 ttctaatatg gttgaaaatc gagaggggta tataataaca taagaccgta aacaatatat
     8641 cgggtttgcc gtccgtaccg accgtatacc gcagcctgcc cgtccacaaa cccatgtcct
     8701 tgacccgcct aatcttgaaa ttctatgcac tattgcgcct ttttttgggc aaaaacgccc
     8761 gcaccgcatg gatttcgcat cccgcctgtg ccgggcacga acccggcgca aaccatcccg
     8821 attcgcccga ccgcatcctc tgcatcgagc aggcattgcg ccgcgccagt atttggcagc
     8881 acctccaaac catagaggcg gaagaaatca gcgatacgcg cctcgcactt gtccactcga
     8941 gcaaatatct gaaccgtttg gaatcttgcc tgccccaaaa aggcaagatt tcccgcctgg
     9001 ataacgacac tgcaatcagc acaggctcgc tgtctgccgc acgctttgcc gccggttcgg
     9061 cagttcaggc agtcgacatg gtcatgaacc gtaaagcatg gcatgccttt tgcgccgccc
     9121 gcccgcccgg acaccatgcg ggcagcggca aagccggcgg attctgcctg ctgaacaacg
     9181 ttgccgccgg cgtcatgcat gccattgccg aataccgcct gaaacgcatt gccgtcatcg
     9241 atttcgatgt ccactacggc gacggtacgg cagaaatatt caaagacgat ccgcgcatcc
     9301 tgtttttcaa cctgtttgaa accgaccttt ttcctttccc cgaaaacaac gatatgcccg
     9361 acggcggcaa tatggtgcac ctgcccttgc cgccgggaac gggcagccgc acattccgcg
     9421 aagccgtccg cagacagtgg ctgccccggc ttgccgcatt caaacccgaa ctggtgctgc
     9481 tgtcggcagg attcgacgca caccgtctag acgaatcggg caggctcaac ctgcacgagg
     9541 cggattttgc ctggctgaca cacaaaatca ttcagacggc atcgggctgc cccggcaaaa
     9601 tcgtatccgt actcgaaggc ggctacaccc ttgaacctct ggcacaatct gctgccgagc
     9661 atatccgcgt gctggcaggg ctgggcaaat ccgatgccgc aaccgcctat cagaaaacac
     9721 tggacccaac aaaaaaacgg tttgccaaac cgaaaaccgg acaggtgcgc caacctaccc
     9781 aatccgaccg ttac
//
LOCUS       DAWKOG010000064         9766 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.064, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000064 DAWKOG010000000
VERSION     DAWKOG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9766
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.064"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(312..545)
                     /locus_tag="WJK66_001156"
     CDS             complement(312..545)
                     /locus_tag="WJK66_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEZ7578874.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQNEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(711..911)
                     /locus_tag="WJK66_001157"
     CDS             complement(711..911)
                     /locus_tag="WJK66_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HEZ7578875.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(1083..1958)
                     /gene="xerD"
                     /locus_tag="WJK66_001158"
     CDS             complement(1083..1958)
                     /gene="xerD"
                     /locus_tag="WJK66_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEZ7578876.1"
                     /translation="MEEGLIDRLLETLWLDRRLSQNTLNGYRRDLEKIARRLSQSGRM
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTRLLKPPKIDK
                     NIPTLITEQQISRLLAAPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITALGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYASQAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(2010..2450)
                     /locus_tag="WJK66_001159"
     CDS             complement(2010..2450)
                     /locus_tag="WJK66_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEZ7578877.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTLGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
     gene            2771..3709
                     /locus_tag="WJK66_001160"
     CDS             2771..3709
                     /locus_tag="WJK66_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEZ7578878.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRIKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            3837..4130
                     /gene="hfq"
                     /locus_tag="WJK66_001161"
     CDS             3837..4130
                     /gene="hfq"
                     /locus_tag="WJK66_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEZ7578879.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPASTLVQVETVQQPAE"
     gene            4215..4781
                     /locus_tag="WJK66_001162"
     CDS             4215..4781
                     /locus_tag="WJK66_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ7578880.1"
                     /translation="MLLKNILPFAHCLLRQALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALSRTRCRLKEARYENVQLILDGHENLKQYVSEPLNAAIFNFGWLP
                     GGDKSLTTHTETSIPALCATLSLLKEGGMLIAVLYPGHENGKQEAEAIEQWAKNLPQE
                     QFAVLRYGFTNRKNSPPYLLAFEKLRQK"
     gene            4835..5209
                     /locus_tag="WJK66_001163"
     CDS             4835..5209
                     /locus_tag="WJK66_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEZ7578881.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRFFLLWKNPEKPLAGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTV
                     YLLAMCCVACIVYLAKTKVLPF"
     gene            5219..5713
                     /gene="folK"
                     /locus_tag="WJK66_001164"
     CDS             5219..5713
                     /gene="folK"
                     /locus_tag="WJK66_001164"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEZ7578882.1"
                     /translation="MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLHQTSSLY
                     ATAPVGYDDQPDFVNAVCTVSTDMDGISLLSLLNRIEADFGRKRSFRNAPRTLDLDII
                     DFDGISSDDPRLTLPHPRAHERSFVIRPLAEILPDFVLGKHGKVAELSKQLGNQGIRL
                     LPDR"
     gene            complement(5841..6320)
                     /locus_tag="WJK66_001165"
     CDS             complement(5841..6320)
                     /locus_tag="WJK66_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEZ7578883.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYIALSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDRKDSVYKRALSALSRKL
                     GRCKDKLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAVLQI"
     gene            complement(6540..7277)
                     /gene="ubiE"
                     /locus_tag="WJK66_001166"
     CDS             complement(6540..7277)
                     /gene="ubiE"
                     /locus_tag="WJK66_001166"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEZ7578884.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGKLIAKDAESYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(7320..7688)
                     /locus_tag="WJK66_001167"
     CDS             complement(7320..7688)
                     /locus_tag="WJK66_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEZ7578885.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQIADLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            7808..9427
                     /gene="mnmC"
                     /locus_tag="WJK66_001168"
     CDS             7808..9427
                     /gene="mnmC"
                     /locus_tag="WJK66_001168"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEZ7578886.1"
                     /translation="MDNLVWDGIPDIRTLDQAIRKHAPPLNLIICLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTHRLHEHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEYILVIGAGISGAATAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTEQTELLLAGYGYTKRLLGHILPESDTWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITQAEAEKIAGIPLSVPYDHPSCGLYWQHGVW
                     LNPPAFVRALLRHPLIGLHEDTPLTDISHDGEKWIASTPNGTFTATHIIYCTGANSPY
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAHLNPALSESLFAANPNPQKHQGHAAIRCDSPDHLPLVGAL
                     GDIAAMRQTYTKLALDKNYRIDTPCPYLPNAYVNTAHGTRGLATAPICAAAIAAQILG
                     LPHPFSQRLRHALHPNRTIIRAIVRRKDLTP"
     gene            complement(9424..9573)
                     /locus_tag="WJK66_001169"
     CDS             complement(9424..9573)
                     /locus_tag="WJK66_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578887.1"
                     /translation="MDSKGNLLGIPYSLFWEENVMGVFDILPSGLVCLFEYDFLWSGR
                     HAAKA"
BASE COUNT         2615 a         2989 c         1958 g         2204 t
ORIGIN      
        1 taccgattac acaaacacat atcatcttta cacaatcatg cttccatcaa cagtaaaaca
       61 tgatatgatt gccaacaata acatctcaca ataaattttc taatttttat tgaaaaaatc
      121 aataaataag aattctccca accgacaaat aaaataaaga aagggttaat atgcaacatc
      181 gaagaagact agcaatttac caagcatcca aacgtgcttc ctttaccggc aggtcatccg
      241 ccccgcaaaa acgtaaagaa cgttgatttg aaaaaaatgc cgcctgaagt cctgcttcag
      301 acggcatttt tttaccgttc gagaaactgt ttcaacctgt cctcatccaa aaaccagtgg
      361 cagatttcct catctcccgc caacaaaacg ggaaccagct cattgtattt ttcttccaaa
      421 acaggatttt catccacatc gaccacttcc agcccgaacc cgtattcatt ttgaaaaggt
      481 ttgagtgcat cgcgcatttt gtggcacaag ctgcaatatt cacgaaacat caaggtcaat
      541 ttcatccgcg tttccttatc tgtcaatttg cacacgccaa agccttagac gcagcagaat
      601 catggtctat ttgggaaaaa acaatgtttt caaggaagat gatactcaag tcctgccaaa
      661 aacagcaaaa atgccgtctg aacagttcag acggcatttc caaaaccgtt ttacgcttga
      721 acattgatac ccgccgtaac cgtcggttgc gcattgtgcg ccgtcagaaa cgaagcggca
      781 agctccccac agcagccgca gcagctcgct acgcccaatt gcgactgcaa atcgcccatt
      841 gtggtcgcgc cggcggcgat ggtttccttg atttgatggt cggtaacggc attgcagatg
      901 cagacaaaca ttttgtgctc cgtgtgttct caaactatcg taccgatagc ggcttttatt
      961 atcgtatgcg aatataaata aaaacggttc gcattgcaag gtcggtatac acggtttgtc
     1021 ttggtaattt tttatcaagt ttgcattttc gattgatttc ttattaaacc aaagtagaag
     1081 cctcagtttc gggaatggtg ttccttcact acaccctgca accatacgtt ggcaacatgg
     1141 gtataaatct gcgtcgtatt caaatcggca tgtcccaaca tatcctgaac cacgcgcaaa
     1201 tccaagccgt gccgcaccag atgcgtggca aaggcgtggc gcaggctgtg cgggctgatg
     1261 tgcccgatgc ctgcctgact tgcatattct ttgacaatca tccatgccaa ctgacgggaa
     1321 atgcccgtct ttttctgact gacaaacaat gcgtcgcaat tcctgccttt cagcagaagt
     1381 gggcgtgcct ccgtataata gcgttccacc caatacgccg actcctgccc catcgggacc
     1441 atcctctgct tatcaccctt tcccagcgcg gtaatacagc ccctgtccaa atccacattg
     1501 ccgaagttca gcccgaccgc ctcgctgacg cgcaagccgg tcgcgtacat caattcgagc
     1561 aaagccttgt cccgcaaacc gtgcggcgtg tcggtatccg gggcggcaag cagtcgggaa
     1621 atctgctgct cggtgatcag ggtcggaata ttcttgtcga ttttgggcgg tttcagcaaa
     1681 cgggtgggat tgtccgtcct tatgccttca cgctccatcc atatatacag gcgtttgcat
     1741 gccgataatg cgcgcgcctg cgaactccgt tgctctccgt caacataaac cgccgccgcc
     1801 aaatccgctt cgtccgcatc cttcagcatt ctgcccgatt gggacaggcg gcgggcgatt
     1861 ttttccaaat cgcgccggta accgtttaaa gtattctgac tgagccgcct gtccaaccac
     1921 agcgtttcaa gcagcctgtc gatcaaacct tcttccatac cgttccaaac aaatgccgtc
     1981 tgaatcttct tcagacggca tggttcacat tatcgggaaa gcgtttccaa tacttcctgc
     2041 gcgtgacccg ccactttgac tttccgccat tcatgggcga tttctccatc cttattcaag
     2101 acgaacgtac tgcgctcgat acctaacgac tctttcccgt acagtttctt caatttgatg
     2161 acatcaaaca ggcggcacac tgtttcatcc ttgtcgctca acagctcgaa ccggaaaccc
     2221 tgcttggcgc aaaaattctg atgcgccttt acgccgtcgc gggaaatacc gaccacggta
     2281 taacccaatg cctcaaactg ttccaaacgc gcattgaaat ccaagccttc cgtcgtacag
     2341 cccagcgtac tgtctttcgg ataaaaatac acgaccaaag gcagatgttc tgccgaatga
     2401 aaatccgcac cgctgctcga aggcagggta aattcatatt tcacatccat agtcctactc
     2461 ccgatattcc cattattcaa aacgacacgc agacgcaccg ccgcaatttc caaaccaacc
     2521 ccgattctac cgccccaaag gacagggatt caaccgccgg aaacatccaa accgacacac
     2581 gacggcatga aaaatatcca tgtcaaacca caaaatatgt tccgatttaa aaacagaatg
     2641 ttataaaacc caatccccaa aacacaacaa gaccgcccgc ctacgggcag tctcctgtca
     2701 gacgacatac tttacagatg gctgtttttt caacaaaata acgccaatac tcaaaaatat
     2761 ggaatcaaaa atgtccatcc atactctgaa acgcctgccc tcatcgctgc tgctcggtct
     2821 ctgcctttcc ctgccgtcag cccacctttt tgccgacaac gacattttag ggcaattttt
     2881 agaacagaac atgcttacct cctccgatcc gatagaaata ttcgccgaaa gcacgataca
     2941 ccccaccaac acccaagcca ttacaggcgg tctgattctc tcctcacagt ctgccctggt
     3001 cgtcaacaac aaaaccggac agatactgta tcagaaaaac gccgacagga ttatgcccat
     3061 cgcctccatt tccaaactga tgagcgcgat ggtcgttttg gatgcaaact tggacatgaa
     3121 cgaaaccgtt accattacgc ccgacgaaat cgaccgcatc aaagggaccg gcagccgtct
     3181 tgccataggt acggcactta cacgcaaaga actgctgcac ctgagcctga tgagcagcga
     3241 aaaccgcgcc acccatgcat tgggcagaac ctaccccggc ggcatgggcg catttgtcgc
     3301 cgccatgaac cgcaaagccc aaagcctcgg tatgtacggc agccgctttt acgaaccgac
     3361 cggactcaac ttccaaaacg tttccaccgc caaagacctg agccttatgg tcaacgccgc
     3421 cgcccaatat ccgcaaatcc gcaccaactc gacttccaac tacgcctcgg tacagaccaa
     3481 aaacgggcag cagaactaca aaaactccaa tgccctggtc agagaaggca tgtggaacat
     3541 cgaattgcag aaaaccggct acatacgcga agcaggcagg tctatggttg tcaaagccaa
     3601 cattcaaaac caacccgtta ccatcgtatt gctgaactcg cccacatccg ccacacgcgt
     3661 caacgacgct cgcaaaatcg aatcgtggat gctgcagcaa cgctcctgac atacaaatgc
     3721 ccggcggaaa accgcccaaa cacaaaaaaa ttggtaattt ttcctattca aggctacaat
     3781 tcgacacgca cagggcgtct gcccgatttc acaatcataa taacggagtt aaaaatatga
     3841 cagctaaagg acaaatgttg caagatccct tcctgaacgc cctgcgtaaa gagcacgttc
     3901 cggtttcgat ttacctggtc aacggcatca aactgcaagg tcaggttgaa tctttcgacc
     3961 aatacgtcgt tcttctgaga aacacttccg taacccaaat ggtttacaaa cacgccattt
     4021 ccaccatcgt accggctcgc tccgtcaacc tccaacacga aaacagaccc caagccgcac
     4081 cggcttcgac ccttgtccaa gtggaaaccg tccagcagcc tgccgaataa tacgtacgaa
     4141 gcatgacgtg tcatatcttt caatacctta ccggacaacg gtaaggtatt tttattttca
     4201 gacggcattt gaaaatgtta ttgaaaaaca tccttccatt cgcccattgc cttttgcggc
     4261 aggcacttcc cgaaggcggc aatgcgctgg acggcaccgc cggcaacgga cacgacaccc
     4321 ttttcctcgc acaaaccgca ggcatccggg ggaaagtgtg ggcattcgac atccagccgc
     4381 aagccctgag ccgcactcga tgccgtctga aagaagcgag atacgagaat gtgcaactca
     4441 tcttagacgg acacgaaaac ctgaaacaat atgtttcaga accactaaat gccgccattt
     4501 tcaactttgg ctggctgccc ggcggagata aaagccttac cacccatacg gaaaccagca
     4561 ttcccgccct ttgcgcaacc ctctccctgc tgaaagaagg cggtatgctt attgccgtcc
     4621 tctatccggg acacgaaaac ggcaaacagg aggcagaagc aatcgaacaa tgggcaaaaa
     4681 acctgcctca agaacagttt gccgttttgc gttacggctt taccaaccgg aaaaacagcc
     4741 cgccctatct tttggcattt gaaaaactgc gtcaaaaata actgtttgcg gtaaaataag
     4801 ccgattctaa cccttatttt gatacgagcc gaccatgcag tatctgattg tcaaatacag
     4861 ccatcaaatc ttcgttacca tcaccatttt ggtattcaac atccgttttt tcctactttg
     4921 gaaaaatcca gaaaagccct tggcgggctt ttggaaagca ctgccccacc ttaacgacac
     4981 catgctgctg tttacgggat tgtggctgat gaaaattacc catttctccc cgttcaacgc
     5041 gccttggctc ggtacaaaaa tcctgcttct gctcgcctat atcgcattgg gtatgatgat
     5101 gatgcgcgcc cgtccgcgtt caaccaagtt ctacaccgtt tacctgctcg ctatgtgttg
     5161 cgtcgcctgc atcgtttacc ttgccaaaac caaagtcctg ccgttctgaa acaccgttat
     5221 gaacaacaga cattttgccg tcatcgccct aggcagtaat cttgaaaacc ctgcccaaca
     5281 ggtacgcgcc gcattggaca cgctgtcgtc ccatcccgat attcggcttc atcaaacctc
     5341 gtccctgtat gccaccgccc ctgtcggcta tgacgaccag cccgactttg tcaatgccgt
     5401 ctgcaccgtt tccaccgata tggacggcat ctcgctgctc tccttgctca accgtatcga
     5461 ggcggatttc gggcgcaaac gcagtttccg aaacgcgccg cgcacattgg atttggacat
     5521 tatcgacttt gacggcatct ccagcgacga cccccgcctc accctgccgc atccgcgcgc
     5581 acacgaacgc agtttcgtca tacgcccttt ggcagaaatc ctccctgatt ttgttttagg
     5641 caaacacgga aaagttgccg aattgtcaaa acaactgggc aatcaaggta tccgtctttt
     5701 accggacagg taattccgca cacagatgcc gtctgaatgc cggtttcaga cggcattttt
     5761 accctgccgc caatctgtac gcaaaaaaaa tcgccccttg aaaaaggggc gcaaaaggaa
     5821 cacaaaccac taccaaaact ttaaatctgc aacactgcct gccgcatact gtatccgaca
     5881 ggatataaag ccctcaccaa atcgtttcga gaaatccaaa cttcttcatc gccgacagaa
     5941 aacctgcctt tctccggcac cagctccaac aaaaacggct gaaccgccgt atgcagcttg
     6001 tccttacacc gccccagctt tctggacaac gcggacaagg cacgcttata gacactatcc
     6061 ttgcggtcaa gttcccgcgc actgccgacc cagctcaaac gaaggttgcg gctttcatcc
     6121 tcaatcagca aaatgcctgt tttgacttcc cccacctcgg gctcacatga aagcgcaata
     6181 taatatttgc cgtcgatgtt gacatacgcc gcttcatgca cctgacagtt gcagccggaa
     6241 gtggcaagca gcgaataaac agagtcccga tgcagtttca aaatatcaag ggaaatctgt
     6301 ttgaaaatac tttccggcat ttttccctcc tttcgtctgc tgcgttgttt actggattga
     6361 ctgcattata cagacatctc ccaatcttgc aaatgataat tgttcctatt ttttaaaaaa
     6421 tttataaatt attgatggat atgtgaatta attttaagat ttaaaatctc tttctcaaaa
     6481 aaacacaaaa atgccgtctg aaacgtgttc agacggcatt ggctgcacag ccagtccgtt
     6541 taaaatttca cgcccttatg cagcgcgacg atgcccgcac tcatattgtg ataatccacg
     6601 ctgtcaaagc ccgcatccag catcatctgt ttcaaagttt cctgatcggg gtgcatacgg
     6661 atggattcgg cgagatattg ataactctcc gcatctttgg caatcagttt gcccatgacc
     6721 ggcagcagct tgaacgaata gaaatcatac gcgccttcca aaggtttgta gattttggaa
     6781 aactccaata ccagcaatgt cccgccgggt ttcaaaacac ggtacatctc tttcagcgcg
     6841 gcatctttat gcgtcatatt ccgcaaaccg aacgccacgg aaaccaagtt gaaataattg
     6901 tcagggaaag gcagtttttc cgcatcggca agcgacacgg gcaaaatcat gccttcgttc
     6961 aaaagacggt cgcgcccgac ggtcagcata gaggaattaa tatcggtcag ccaaacctcg
     7021 ccttccttgc cgacccgttt cgcccaaccg cgcgacaaat cgcccgtacc gcccgcaatg
     7081 tccaacactt tatcgccttt tttcaggtgc gccgtgttga tggtgaaatg cttccacacc
     7141 ctgtgcagcc ctgccgacat cacatcgttc ataatgtcgt agtttttggc gacggagtgg
     7201 aacacttccg ccactttgcc ggctttctca tcttcgttga ccgtactgaa tccgaaatgc
     7261 gttttctgtc cgcccatatt ctaaccacct ccctgatgcc gccctacggc atcccaaact
     7321 taacaatctg attgcttttc aaaacgggaa gcacccgccg ccgccaattt gtccaaatat
     7381 tcctgccaca tcgcatcgta tccgtatgcc agtctgtgca gatacgccca atcgtaaaga
     7441 ccgctgtcgt gcccgtctga aaaactgatt ttcagcgcgt actgtccgac aggctgcaaa
     7501 tccgcgattt ggacatccgc cttgccggtc tgcaaaacat cctgtcccac gccgtgtccg
     7561 cgcacttccg cactcggcga atagacgcgt aaaaattcgg caggcagatt tttaggttcg
     7621 tccccataag tcaaaaccaa cacggtacgg tttttcaaca aacggatttc aacaggaata
     7681 ttattcaaaa aacacctcgg ttcggcatac aatatccggc aggcgcaggc gtgccttcaa
     7741 acggcgcgta ttttaacagc tttatcccga taccgatatt caaacccaca ctttaaacgg
     7801 cattgatatg gacaatctcg tatgggacgg cattcccgac atccgcacac tcgaccaagc
     7861 catccgcaaa cacgcacccc cgctcaacct gattatctgc ctccccgata atcagattcc
     7921 cgattttcaa accgcacaag atgcttcgga cgcggaatgc cgtctgaagc accgtttgga
     7981 tcaggcaacg cagtgcctcc agttcgacag catcaacctc atcgaacaca tcctgcccga
     8041 tgtccgcttc tggctggttc ccccttcacg cacccaccgc ctgcacgaac acttccacca
     8101 catttcctgg cagaccgaag ccatcccgca aaccgaaagc aaacccgaca aaccctggtt
     8161 tgcacttcca caaacatccg aacggcaaaa accggaatac atcctcgtta tcggcgcggg
     8221 catatccggc gcggcaaccg cccacgcctt agcatcacac ggcatttccg ttaccgtatt
     8281 ggaagcccga aaagccgccc aagccgccag cggcaaccgc caagggctgc tctacgccaa
     8341 aatctcgccg cacgacaccg aacaaaccga actgctgctt gccggctacg gctacaccaa
     8401 acgcctgctc ggacacatcc tgcccgaatc cgacacttgg ggcggcaacg gcatcatcca
     8461 cctcaattac agccgcaccg aacaacaacg caatcacgaa ttgggtttgc aaaaacacca
     8521 taaccacctc taccgcagca tcacgcaggc agaagccgaa aaaatcgccg gtatcccgct
     8581 gtccgtccca tacgaccacc cttcatgcgg actctactgg caacacggcg tatggctcaa
     8641 tccgcccgca ttcgtccgcg ccctcctccg ccatccgctg atcggactgc acgaagacac
     8701 accattaacc gacatttccc acgacgggga aaagtggatt gcaagcacgc caaacggcac
     8761 atttaccgcc acacacatca tctactgcac cggtgcgaac agcccctacc tacccgaaac
     8821 caacctcgcc gccctgcctc tcaggcaaat acgcggacaa accggcctca caccgtccac
     8881 cccgttttcc gaacaactgc gttgcgccgt ttcaggcgaa agctacatca gcccgtcgtg
     8941 gcacggactg cactgctacg gcgcgagttt tattcccaac agcagccata ccggatggaa
     9001 cgaagccgaa gaagcctcaa accgccaagc attggcacac cttaaccccg ccctttccga
     9061 atcattgttt gccgccaacc caaaccccca aaaacaccaa gggcacgccg ccatacgctg
     9121 cgacagcccc gaccaccttc ccctagtcgg cgcactcggc gacattgccg ccatgcggca
     9181 gacctacacc aaactcgcgc tggacaaaaa ctaccgcatc gacaccccat gcccatacct
     9241 gcctaatgcc tacgtcaaca ccgcgcacgg cacccgcgga ctcgccaccg cccccatctg
     9301 cgccgccgcc attgcagccc aaatcctagg cttgccccat cccttttcac aacgcctgcg
     9361 ccacgcccta caccccaacc gcaccatcat ccgcgccatc gtcagaagga aggatctaac
     9421 cccttaagcc tttgccgcat gccgtcccga ccacagaaaa tcatattcaa ataaacatac
     9481 caatcctgac ggtaaaatat cgaaaacacc cataacgttt tcttcccaaa ataaggagta
     9541 aggaatgcct aacaaattgc ctttgctgtc caagatgaat gttccgtaac attcgaagac
     9601 ttccttaacc ctgccagccg tttcggtttc tgtaccccct gttagataag gaaggaatga
     9661 aaaagccctg ttgattgtat gaagtaaagg gcgttaattt ttgttaatct tcccttctca
     9721 ggaactcaat atataaggat gttaattttt atttcccttc tcgagg
//
LOCUS       DAWKOG010000065         9754 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.065, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000065 DAWKOG010000000
VERSION     DAWKOG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9754
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.065"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..252)
                     /locus_tag="WJK66_001170"
     CDS             complement(<1..252)
                     /locus_tag="WJK66_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578888.1"
                     /translation="MTAMPSEPPFRRHFTHQNRKTAPKIIITKHNGKPDTKPNTAAKM
                     PSEAVGATILIWGPLQGRITFLNHPFDSQINSAFACQKQN"
     gene            complement(249..1304)
                     /locus_tag="WJK66_001171"
     CDS             complement(249..1304)
                     /locus_tag="WJK66_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7578889.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKATNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSVVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDTFSPEKKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            complement(1410..1892)
                     /locus_tag="WJK66_001172"
     CDS             complement(1410..1892)
                     /locus_tag="WJK66_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ7578890.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            complement(1939..3075)
                     /locus_tag="WJK66_001173"
     CDS             complement(1939..3075)
                     /locus_tag="WJK66_001173"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEZ7578891.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPNPNA
                     PAAEFADRHLCAPFDNQTALEELAKCAAVTTEFENVNADAMRFLAKHTNVSPSGDCVA
                     IAQNRIQEKAWIRKAGLQTAPYQAICKAEDITEESIQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHRGVDCVLEKMVDLRGEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADIQQQARQMAQRLADELNYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTVDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVWQEDGGEPDWFPLQS
                     RPDAHLHLYGKKTAHKGRKMGHFTVLSTDSDTAFQEAKKLHQSL"
     gene            complement(3072..3617)
                     /locus_tag="WJK66_001174"
     CDS             complement(3072..3617)
                     /locus_tag="WJK66_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEZ7578892.1"
                     /translation="MTYEKLYELRQTLRPTTKDGLYTDNEKSREILTVRWSGNTENPK
                     NFSAVAIGINPSKANDERSDKTLTQLARFLDMYGFTNFKMLNIFSSYSTQQTGIRANT
                     QTDFSKFKGCLEDADMIILAWGTDRSAYKDEKNRILEFLKAEKFMEKVFCISETGNSS
                     DTRHPSRISYSYQLVQFEESA"
     gene            complement(3614..5089)
                     /locus_tag="WJK66_001175"
     CDS             complement(3614..5089)
                     /locus_tag="WJK66_001175"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEZ7578893.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVIDNLSGKIHLVVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSDPASEWQETQNKARAVIRAAQMVQEGLDK"
     gene            complement(5200..5565)
                     /locus_tag="WJK66_001176"
     CDS             complement(5200..5565)
                     /locus_tag="WJK66_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578894.1"
                     /translation="MFKRPEEIIVLILAVLWIAGTYFLAALFGADAYTVLKITALTLL
                     WSAASFLLWQKNLSRHIWPLLLGCLTACWWPYLNQSVAPGSLPWYASWTFKTILALIP
                     VTAGYLFKWKQYRQRKTKP"
     gene            complement(5586..7496)
                     /locus_tag="WJK66_001177"
     CDS             complement(5586..7496)
                     /locus_tag="WJK66_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ7578895.1"
                     /translation="MNILSVENASFAVGHVALLDKTSFQLDRGEKIGLIGRNGAGKSS
                     FLKILAGVQKLDDGQIIVQNNLKIVYVPQESFFDKEATVFDTVAEGLGEIRDLLRRYH
                     HVSHELENGSSEALLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGEKSGKIIAELEHASFTYGDKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     TLNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFHPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILILDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     IFEGNGRLKEYIGGYQDYIDAKSREAKIQTESAPKTADAEPVKEKPKANRTVKLSYKE
                     QRELDALPDEIAALEAEQAEINAQLSDPEIFKDYEKAGALQSRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            complement(7515..8159)
                     /locus_tag="WJK66_001178"
     CDS             complement(7515..8159)
                     /locus_tag="WJK66_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ7578896.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFMPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            complement(8323..9141)
                     /gene="opc"
                     /locus_tag="WJK66_001179"
                     /gene_synonym="opcA"
     CDS             complement(8323..9141)
                     /gene="opc"
                     /locus_tag="WJK66_001179"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
                     /protein_id="HEZ7578897.1"
                     /translation="MKKTVFTCAMIALTGTAAAAQELQTANEFTVHTDLSSISSTRAF
                     LKEKHKAAKHIGVRADIPFDANQGIRLEAGFGRSKKNIINLETDENKLGKTKNVKLPT
                     GVPENRIDLYTGYTYTQTLSDSLNFRVGAGLGFESSKDSIKTTKHTLHSSRQSWLAKV
                     HADLLSQLGNGWYINPWSEVKFDLNSRYKLNTGVTNLKKDINQKTNGWGFGLGANIGK
                     KLGESASIEAGPFYKQRTYKESGEFSVTTKSGDVSLTIPKTSIREYGLRVGIKF"
BASE COUNT         2113 a         2150 c         2759 g         2732 t
ORIGIN      
        1 gttttgtttc tgacaggcaa aagcactgtt tatttggctg tcaaaaggat ggttaaggaa
       61 agttatgcgc ccctgaagcg ggccccagat aaggatggtt gcgccgacgg cttcagacgg
      121 cattttggcg gcggtgttgg gttttgtatc cggtttgccg ttgtgttttg tgatgatgat
      181 tttgggcgcg gtttttctgt tttgatgtgt gaaatgccgt ctgaaaggcg gttcagacgg
      241 catagcggtc atttttgtgc ggtcaggcgg tcgaatatgc cgccgtcggc gaagtaggtt
      301 ttcatgatgt tgtcccatcc gccgaatttt ttttcgggag agaaggtgtc taagtctggg
      361 aagtcggctt tgtgtcttgc caatacttcg gggttgcggg ggcgcaggta gagtgaggcg
      421 gcgagttctt gcgccggttc gctccaaagg tattcgagat aggcgcgggc ggttttttgc
      481 gtgccttttt tcgcgacgac gctgttgacg acggcgacgg ggctttcggc ggaaatggtg
      541 tagctcggat agacgatttc aaactgtcct tgggtcagtt ttttgctgac gtagttggct
      601 tcgttttcaa aagtgatgag tacgtcgccg atgttgcgtt gtgtgaaggt ggtggtggcg
      661 gcgcgtccgc cgttttcaaa aacgggggtg tttttgagga tggatgcgac gagtttttgg
      721 gcttcctgtt cgttgccgtt ggtggctttc agaccgtaac cgtatgcgcc gaggaaggcg
      781 tagcgtccgt tgcccgaggt tttgggattg gcgatgacga tgttaacgcc gtctttggca
      841 aggtcgttcc aatcgcggat ctgtttgggg ttgttttttc ggacaaggaa aaccatagtg
      901 ctggtgtagg gcgcggcgtg gtcggggagg gcttgttgcc agcctttttc taccagtcct
      961 tttttttcga gcaggtcgat gtcggaggat tggttcatgg ttacgacatc ggcttgaagg
     1021 ccgttggcta cggataatgc ctgtttgctg gagccgccgt gggactgttg gatgctgacg
     1081 gatgtgccgg ggtgttcgga ttggtatgtt ttgataaata aggggttgta ttctttgtaa
     1141 aaatcccgtg ccacatcgta tgaggcgttg agcagggtaa tgttttttcc gtcggattcg
     1201 gtattggccg gggcattttg tccggacgga tggtttgaat cggctgcggg gctgcaggcg
     1261 gtgagcaggg ctgcggtata gagtgccggt gcgtaggttt tcatatgctt gtcctgtcgg
     1321 ttggtagatg gggcaacttt atacggctgt ctgcgcttgt ggaaataatg tttgatttga
     1381 agattatcag ttttggttat aaggacggat cagaggtgtt tccgcatcag ttcgcatttg
     1441 attttgatgc tggggtcaag ctgcaatact gccgaaccga gcgattcgta gcggttgaga
     1501 aggtaaaacg ggacggagtt gagcgatgcg tagagtttca gaaagctcaa accggatttg
     1561 tgggcgatgg tttccgcctg atgaagcagg gcagtgccca gtccgaggtt gtggaacagg
     1621 gggtggacgt aaagtgcatc gagttgtgct tcttggcagt cgatttggaa aaatccctgt
     1681 atgttgcctt tgtattcggc aacccaaagt gctttgtcgg gatcggaaat ggtcggcagg
     1741 tagctttctg tgtttaacaa gccttcccat acttttaggg cgtgttcgtt gtagctgagg
     1801 atgcaggtgt attggacgga gtgcaggtgg actttgaaga tgtctttgca gtcttgcacg
     1861 gtggcggggc gtaacagtgt cagcaggttc atggcagtat gtcggcggct tcagacggca
     1921 tctgtgccgt tggtcggatt atagggactg atgcagtttt tttgcttctt gaaatgcggt
     1981 gtccgaatcg gtgcttaaaa cggtaaagtg tcccattttc cgccctttgt gcgcggtttt
     2041 tttgccgtaa aggtgcaggt gcgcgtccgg ccggctttgc aggggaaacc aatccggttc
     2101 gccgccgtct tcctgccaaa cgtcgcccaa aatattcgcc atacagcaag aactcagcaa
     2161 tttggtgtca gcaggtggca ggttgcacat caggcggacc tgttgctgga attggtctgc
     2221 cgcgcaggcg tcgacggtat ggtggccgga attgtgcgga cgcggcgcga tttcgttgac
     2281 gaccaattca tgcgtgtcgc cgacaacaaa catttctacc gccaatacgc cgacgtagtt
     2341 caattcatcg gccaaacgct gcgccatttg tcgcgcctgt tgctgaatgt cggcactcag
     2401 tcgggctggg acgatggagt aggcgaggat accgttttcg tgaatgtttt cggcaggatc
     2461 gaaagtttgc acgttgtcat tgttcagacg gcatacgata acggaaattt cgccgcgcaa
     2521 gtccaccatt ttttccaaaa cgcaatccac gccgcggtgt tcggcaaacg cggctttgag
     2581 ttcatccacc gttttgacgc ggatttggcc tttgccgtca tagcccaatg tagcggtttt
     2641 caggatgccg ggcagaaatt gtatgctttc ttcagtgatg tcttcggctt tgcaaattgc
     2701 ttgatacggc gcggtttgca ggcctgcttt gcgtatccat gccttttcct gaatgcggtt
     2761 ttgcgcgatg gcaacgcagt cgccgctggg ggaaacattg gtatgtttgg cgagaaaacg
     2821 catcgcatcg gcattgacgt tttcgaactc ggtcgtaaca gccgcacatt ttgccaattc
     2881 ttccaaagcg gtttggttgt caaacggcgc acacaaatgg cggtcggcaa attccgctgc
     2941 cggcgcattc gggttgggat cgagtacggt tactttgtag cccatggttt tggcagcaac
     3001 agtaaacatt ctgcctaatt gtccgccgcc aagaatgccg agcatggcgg gcggagaaag
     3061 agatatgttt ttcatgctga ctcttcaaat tgtacaagtt gatagctata actaattctt
     3121 gacggatgtc ttgtatcgct ggaattacca gtttcagaaa tacagaatac tttttccata
     3181 aatttttctg cttttagaaa ttccagtatt ctgttttttt catccttata agcactgcgg
     3241 tctgtacccc atgcaagaat aatcatatca gcatcttcca aacatccttt aaatttggaa
     3301 aaatcggttt gggtgtttgc cctaattcct gtttgctgtg tagagtaact ggaaaaaata
     3361 tttaacattt tgaagttggt aaaaccgtac atatccaaga aacgtgcaag ttgggtaagg
     3421 gttttgtcgc ttctttcatc atttgcttta cttggattaa ttcctatagc aacagctgag
     3481 aaatttttag gattttctgt gttgccgctc catctaaccg ttaggatttc tcgacttttt
     3541 tcattatctg tatagagacc gtctttggta gtcggtctga gtgtttggcg aagctcataa
     3601 agtttttcat aagtcattta tccaaccctt cctgcaccat ctgcgcggcg cgtataacgg
     3661 ctcgggcttt gttttgtgtt tcctgccatt cggaggccgg atcggagtcg gcaaccacgc
     3721 ccgcgccgct ttggacgtat agcgtgttgt tttttactac ggcggtgcgg atggcgattg
     3781 ccaaatccat gtcgttgttg aaaccccata cgccgacggc accgccgtag atgccgcgtt
     3841 tgctcggttc gacttcttcg atgatttcca tggcgcggac tttgggtgcg ccggagagtg
     3901 tgccggcagg gaaggtagcg gcgaggatgt ccatgttggt catgccgtct ttcagacggc
     3961 cttcgacgtt ggaaacgatg tgcattacat gggagtattt ttcaatcacc attttgtcgg
     4021 taactttgac ttcgccggtt ttactgatgc ggccgacgtc gttgcgtcct aagtcaatca
     4081 acatgacgtg ttcggcgatt tctttggcat cgcttaacaa atcttgttcg ttggcaaggt
     4141 cttcggcggg ggttttgccg cgcaggcgcg tgccggcgat ggggcggacg atgacatcgt
     4201 cgcgttcgcg gcggacgagg atttcgggcg aggagccgac gatgtggaaa tcgccgaaat
     4261 cgtagtaaaa gagataaggc gaagggttga gcgtacgcag ggcgcggtag agggcgagcg
     4321 ggctgtcggt gaattccatg ctcatgcgct ggctggggac aacctgcatg cagtcgcctg
     4381 cgaagatgta gtctttgatt ttgttaacgc aggctttgaa cggctcttcg ccgaattcgc
     4441 tgacggcttc ggtgtgtttg ctgccgagcg agagcgggat ggcgcagctt tggcgcaact
     4501 gggtgcggat gtcttcgagg cgttcgcggg cgcgttcgta gccgtcgggc tgcgacggat
     4561 cggcgtaaac gacgagatgg attttgccgc tcaaattgtc gatcaccgcc aattcttgcg
     4621 acagcatcag caagatgtcg ggcgtgccga gcgggtcggc tttggtggtg tttttcaggc
     4681 ggtgggcgaa gtgttcaaaa ttgtagatgg tttcgtaacc gaagtagccg accagtccgc
     4741 cggtaaagcg cggcaggctt gggatttcgg gtgttttgaa gcggttgtgg aaggcttcga
     4801 taaagggcag ggggttgccg tcgtgttgct cgacaatttc gccgttttga taaacatcga
     4861 cgtgtttgcc gctggctttg agatagtggc tgcaaggcag gccgataaaa gaatagcggc
     4921 cgaaacgttc gccaccgaca acggattcga gcaggtaggt ataggggcgg ttggcgagtt
     4981 tgagatagag ggaaagcggc gtatccaaat cggcaaggag ttcttgcacg agcgggatgc
     5041 ggttgtagcc ttgggcggct tgggcttggt attcttgttt gctgatcatt tctgctttcc
     5101 caaagggcgg tttcggacgg cgcggcaacg ggcgcgagta tagcatttta tcggaattgt
     5161 tgacagtctg accggagatg ccgtctgaaa aggacgggct tacggctttg ttttgcgctg
     5221 ccggtattgt ttccatttaa acagataacc tgcggttacg ggaatcaggg ctaagatggt
     5281 tttgaacgtc catgatgcat accaaggtaa agaaccgggt gcgaccgatt gattcagata
     5341 cggccaccaa caggcagtca ggcagccgag cagcagcggc cagatatgcc ggctgaggtt
     5401 tttttgccaa aggaggaatg atgcggcaga ccaaagcagg gtaagggcag tgatttttaa
     5461 cacggtataa gcatccgctc caaacagggc ggcgagaaaa tacgtaccgg caatccagag
     5521 gacggcaagt atcaggacaa tgatttcttc aggtcgtttg aacatttttt tcttcctgtt
     5581 tgatttcaga cggcattgcc gttctgtttg gtttccagca gctcccagcg ttccagcttt
     5641 tccaaaagca gcatttcgat ttcttcggcg cggctttgca gtgcgccggc tttttcgtaa
     5701 tctttgaaga tttcgggatc ggaaagctgc gcgttgattt ctgcctgttc ggcttccaag
     5761 gcggcgattt cgtcgggcag ggcgtcgagt tcgcgctgtt ctttgtagga cagtttgacc
     5821 gtgcggttgg ctttgggttt ttctttcacc ggttcggcat cggctgtttt gggtgcggat
     5881 tccgtctgaa tcttcgcttc gcgtgatttt gcgtcgatgt agtcctgata gccgccgata
     5941 tattctttta accttccatt tccttcaaaa ataatgcttt gggtaatcac gttatcgagg
     6001 aacatacggt cgtgtgagac aaggaatacc gtgccttgat aatcgcgcag caaatcttca
     6061 agcagctctt gggtatcgat atccaagtcg ttggtcggtt cgtccaaaat caaaatatta
     6121 gcaggacggg taaagagttt tgccagcaaa aggcggttgc gttcgccgcc ggagagcgat
     6181 gaaacgggac tttgtgcgcg ggctggatgg aataagaaat cttccagata gctcattacg
     6241 tgttttttct tgccgccgac ttcgacgtaa tcgttgcctt gaccgagcgt gtaaaacacg
     6301 gtgtcgtttt cgttcaacgt gctgcggaat tggtcgaaat aggcaacttc ctgcttactg
     6361 ccgatacgga ttctgccgta ggtcggctgc aattcgccca aaatcagctt gaggaaggtg
     6421 gttttgccga taccgtttgg gccgattaag ccgattttgt cgccacgctg caagatggcg
     6481 gagaatttgt ccatgatgac tttgtcgcca taggtaaacg aggcatgttc cagttcggca
     6541 atgattttgc cgcttttctc gccgctgtcg agcttgaaat tgacctgccc ttgtacgttg
     6601 cggcgttcgg cacgctggcg gcgcagttct tccaaacggc gcacgcggcc ttcgttgcgg
     6661 gtacggcgcg cttcgatgcc tttgcgtatc cacgcttctt cctgcgcgtg gaatttgtcg
     6721 aagaggcggt tgtgttccgc ttcgactgcc aactcttgcg cttttttctc gctatatttg
     6781 gagaacgagc cgggatagga acgcaaaata ccgcgatcga gttcgacaat ccgcgtggcg
     6841 atattgtcca aaaaacggcg gtcgtgggta atcacaacca ggctgccttc aaacgctttg
     6901 agcaggtttt ccaaccaaat aatcgcgtcg atgtccaaat ggttggtcgg ttcatcgagc
     6961 agcaatacgt cgggcttctg cacccaagcc tgcgccaagg cgacgcgctt tttctgaccg
     7021 ccggagaggt tgccgatttt ttcgttttcc ggcaaaccga gttcgcccaa agtctgcttc
     7081 accgccgcat ccaacttcca gccgtccttc gcttcgatct caagttgcaa ttcgttgagc
     7141 tctttcaata aggcctcact cgaaccattt tccaactcat ggctgacatg atgataacgg
     7201 cgcaataaat cgcgaatttc gcccaaacct tcggcaacag tatcaaatac ggttgcttcc
     7261 ttatcaaaaa aggattcctg cggtacataa acgattttga ggttgttttg gacaataatc
     7321 tgcccatcat caagcttttg cacgccggcg aggattttta aaaacgaaga cttacccgca
     7381 ccattacgac cgattaagcc gattttttcg cctctgtcga gttggaagga agttttgtcg
     7441 agcagggcga cgtggccgac ggcaaaggaa gcgttttcta cagataatat attcatgata
     7501 caaattctca acagttaccg tttggatttt accgcaagtt tggcgcgggc aatttcaacc
     7561 gcacccggca ggacggaaac aataatgatg ccgcccatca ccaagcccag attgtttttt
     7621 acgacgggga agttggcaaa gaaatagccc gcgtaagaaa acaggataac ccacaacaag
     7681 ccaccgatga tgttgtagcg gataaatttg gcatagtgca ttttccccat accggcgacg
     7741 aagggggcga aggtgcggac gatgggcata aaacgggcaa tgatgatggt tttgccgccg
     7801 tgtttttcgt aaaaacggtg ggttttatcg agatattcac gtcggaagat tttagaatcg
     7861 ggattggcga acagcctgcc gccgaaatat ttgccgacgg taaaattgag cgcgtcgccg
     7921 agtatggcgg caaggcttaa taatgcaacc atcaaatgaa tatccatacc gcccagcgcg
     7981 gcaataccgc cggcggcaaa cagcagcgaa tcgccgggca gtaagggcgt aacaatcagg
     8041 ccggtttcgc aaaaaacaat caaaaacaga atcgcataaa tccacacacc gtattgcgcc
     8101 gacagcgcga gcaggtgttg gtcgatatgg aggatgaagt cgatggcgga ggcaagcacg
     8161 gcgcgttcct aaaaaaaaca aaccgcgtat tttaaccgat tggaaaaatg ccgtctgaaa
     8221 ttttcagacg gcatcggcta ttcaaattca tttcacgtaa aaaccgcaaa ccaaaatagt
     8281 ttgcggtttg gcatttaaag tgacaatgat gatttcaaat catcagaatt ttatgccgac
     8341 gcgcaagccg tattcacgaa tactggtttt cgggatggtg agcgatacgt cgccactctt
     8401 ggttgttaca ctaaactcgc cggattcttt gtaagtgcgt tgtttgtaga acggccccgc
     8461 ctcgatgctg gcggattcgc ccagtttttt accaatattt gcacccaatc caaagcccca
     8521 gccgttggtt ttttgattga tgtctttttt gagattggta acgccggtgt ttaatttata
     8581 gcgggaattg aggtcaaatt tcacttcaga ccaagggttg atataccagc cgttacccag
     8641 ttgggaaagc aaatccgcgt gaactttggc taaccacgac tgacggctgc tgtgaagcgt
     8701 atgcttggtg gttttaatgc tgtcttttga agattcaaaa cccaagccgg cacccacacg
     8761 gaaatttaaa gaatcactta acgtttgggt gtaggtgtag cctgtgtaaa gatcgatacg
     8821 gttttcagga acgccggtgg gcagttttac atttttagtc ttacccagct tgttctcatc
     8881 tgtttccaaa ttaataatat tttttttgct gcgcccgaaa ccggcttcca agcggatgcc
     8941 ttggttggca tcaaaaggaa tatcagcacg tacgccgatg tgtttggcag ctttgtgttt
     9001 ttctttcagg aaagcacgag ttgaagaaat ggaagagagg tcggtgtgga cggtaaactc
     9061 attagcggtt tgaagctctt gtgcagcggc ggcagtaccg gtcagggcaa tcatggcaca
     9121 tgtaaaaact gtttttttca tagttaaaat ctccaaaatt tggattgtag tcggatatgg
     9181 taacataacg taaataatcg ttacgcttac aattatattc ttaagctttc gggggggggg
     9241 gattttacat atattaataa aaattaacaa atagttattt gtttacaacg aattgttatt
     9301 ctcacttggt tttctgtttt ttatgggaat gacgaaattt taggtttctg atttcggttt
     9361 tctgttttaa gggaatgacg agacttgaga tggcggcatt tatcgggagc aactgaaacc
     9421 actctgccgt cattcccgcg aaagcgggaa tctaggttcg tccggtttcg gttatttccg
     9481 atagattcct gccgcgttgg gggtctggat tcccgcctgc gcgggaatga cgaatttcag
     9541 gttgctgttt ttggttttct gtttttgtga aaataacagg atgagatctt tgcaaaattc
     9601 ctttccctcc cgacagccga aacccaaaca caggttttcg tctattttcg ccccaaatac
     9661 ctcctaattt tacccaaata cccccttaac cctccccgga tacccgataa tcaggcatcc
     9721 ggtctccttt taggcggcag cgggcgcact tagc
//
LOCUS       DAWKOG010000066         9753 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.066, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000066 DAWKOG010000000
VERSION     DAWKOG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9753
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.066"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(35..760)
                     /locus_tag="WJK66_001180"
     CDS             complement(35..760)
                     /locus_tag="WJK66_001180"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEZ7578898.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSGDLDAGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVVFPDRLHIPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGYAHSFECHYPDESGETRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(830..1264)
                     /gene="fur"
                     /locus_tag="WJK66_001181"
     CDS             complement(830..1264)
                     /gene="fur"
                     /locus_tag="WJK66_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEZ7578899.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            1483..1860
                     /locus_tag="WJK66_001182"
     CDS             1483..1860
                     /locus_tag="WJK66_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ7578900.1"
                     /translation="MNKTLILALSALLGLAACSAERVSLFPSYKLKIIQGNELEPRAV
                     AALRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGDVLQNAAEALKDRQNTDKP"
     gene            1870..2679
                     /gene="dapB"
                     /locus_tag="WJK66_001183"
     CDS             1870..2679
                     /gene="dapB"
                     /locus_tag="WJK66_001183"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEZ7578901.1"
                     /translation="MTPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDTGKAAIHTAAEKTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKASSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(2943..3353)
                     /locus_tag="WJK66_001184"
     CDS             complement(2943..3353)
                     /locus_tag="WJK66_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578902.1"
                     /translation="MTTYTIPKKDYQFLYIYEGTLLNYTLKNDEFHIIVQNVDYPDFP
                     QEIPTPNYTDWVKIKFKQFSYLKFIYGYATKNQDKNIKNVLELGELKQDDEILDYGGA
                     LEVIGSRYDLPTGFSIDIVCREIELEFLDQESFN"
     gene            3576..3965
                     /locus_tag="WJK66_001185"
     CDS             3576..3965
                     /locus_tag="WJK66_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HEZ7578903.1"
                     /translation="MNVKIKYNLLEYCEYLGGIFGVYKNYLNMDIKDPNLKIRFFDFL
                     EELLSDGVIELCDYRENPPKILTGSPKTQVDELRRIWPDMEEMLLYFPDNPWFYVEHF
                     WWGATCPIELTQLPKIEIYEEQMKQGK"
     gene            complement(4009..4167)
                     /locus_tag="WJK66_001186"
     CDS             complement(4009..4167)
                     /locus_tag="WJK66_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578904.1"
                     /translation="MDYFPEAARITKKGGEIVINGTSNNKYLRGIPNETELARMGLRL
                     KYNGQLTD"
     gene            complement(4175..5347)
                     /gene="lpxB"
                     /locus_tag="WJK66_001187"
     CDS             complement(4175..5347)
                     /gene="lpxB"
                     /locus_tag="WJK66_001187"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEZ7578905.1"
                     /translation="MWGQTNMADKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARF
                     VGIGGELMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRGLVRDLLSLKPDVFVG
                     IDAPDFNLGVAEKLKRSGIPTVHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLY
                     LDAGGRAEFVGHPMAQLMPLEDDRETARQTLGVDAGIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLERYPAARFLLPAATEATKRRLAEVLQRPEFAGLPLTVIDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQSEAKPEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            complement(5395..6387)
                     /locus_tag="WJK66_001188"
     CDS             complement(5395..6387)
                     /locus_tag="WJK66_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ7578906.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFDVVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEARYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSEDGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAELPPDLAQFAVMLENGTKM"
     gene            6881..9640
                     /locus_tag="WJK66_001189"
     CDS             6881..9640
                     /locus_tag="WJK66_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEZ7578907.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFQDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAQLDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRIIQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTARQEARADTAET
                     RNNGNRRRNERGDRAAERANEAEIQSRNVQPAATVADAAPSETEVQTGKRRRNGSRSE
                     RSQTAPETAAVAETTVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPVA
                     ERDLPTASPAVSNGDAPVYDTAEKIRRATAAILPEGATPKAEAQEMPSETATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQAGRGLRRSEQPKPSEAATVPAEEMIQV
                     ETRQG"
BASE COUNT         2516 a         2994 c         2276 g         1967 t
ORIGIN      
        1 ccatatttga acctgaccgg agcataagcc gccatcaaag ccccttgcac gccacttcgc
       61 gccgcccgat ttcctctttc aacggcacgg cgttcaacat ccgcagacgt tcggcaaaat
      121 ccgcaaacgg cagcagctcc gaaccgaaac ggcgcatatg ttccgtatcc tgctggcagt
      181 ctatcagttc cacgcccaaa tccgccaaaa acggcacggc gcaggcaaac gcgattttcg
      241 acgcatccgg ttgtaatgcg aacatcgatt cgccataaaa caccctgccg atctgaacgc
      301 cgtaaaagcc gcccgccaac ctcgtttcac cgctttcatc gggataatgg cactcgaaag
      361 aatgcgcgta ccccatttcg tgcagcttca aatatgccgt ctgaaactcg ggcgcaatcc
      421 aagttccgtc ctgattcggg cgcgccgctg ccgcacaatg cgcgaccact tccgcaaaac
      481 agccgttgac cgcaacccga tagctgccgt tgcgcagcgt tttcgccagc gagcgcggaa
      541 tatgcagcct gtcgggaaac accaccgcac ggggcccgac cgcataccaa aaaaaccacc
      601 cgtcccggga aaaccacgga aacacgccgt tccgatacgc ctcaagcagc cgccccgcat
      661 ccaaatcgcc gctcacgccg accagcccgt cgcaccgggc caaagcatag gcaggatcgg
      721 gaaaggcata attgtcaggg gcaagcagcg gaatacgcat gacagttctc caaaaacaca
      781 ggcggcatcc aaaatcatga atgccgtctg aacaacaaac cgtccggatt taacgtttgc
      841 ccttggcctg acagtcgctg cacacgccgt acatataaag cgcgtgatcg acgatgcggt
      901 agccgttttc ttccgcgatt ttgtcttgca gggcttcgat ttcgggattg tggaattccg
      961 ttacctcgcc gcacttcacg cagacgatgt ggtcatggtg gtcgcctttg tccaactcat
     1021 aaaccgcctt gcccgtttca aaatgatggc gttgcaaaat gcccgcctgc tcaaactggg
     1081 tcagcacacg gtaaatcgtc gccacaccga tttccacacc ctcttccaac aaaatgcggt
     1141 acacatcttc cgcactcaaa tgctcttccg catgcgtctc gaacaaatcc aaaatcttca
     1201 aacgcgggcc ggtaaccttc agaccgctgt ctttcagttg tgcaatattg ttgaattttt
     1261 ccataatatt caatacccct gtaaaacaat agacgttata atacgcaatt tcggcctgtt
     1321 tgcccactat cgcaccatag cagtttgcaa tagtaaaacc gcaccggcgg ccgatgcgcg
     1381 gacagggcgg ataccgctta atcgtatgat tatcgttcgc ttttataaaa tattcaagca
     1441 gtgttacact acccatcccg atttgcacag aaaggcattt ccgtgaacaa aaccctcatc
     1501 ctcgcccttt ccgccctcct cggccttgcc gcgtgcagtg ccgaacgcgt ttcactgttc
     1561 ccctcgtaca aactcaaaat catacagggc aacgaactcg aaccgcgcgc cgttgccgcc
     1621 ctccgccccg gcatgaccaa agaccaagtc ctgctcctgc tcggcagccc catactgcgc
     1681 gacgcattcc ataccgaccg ctgggactat accttcaaca cctcccgcaa cggcatcatc
     1741 aaagaacgca gcaatctgac cgtctatttt gaaaacggcg tactcgtccg caccgaaggc
     1801 gacgtcctgc aaaacgctgc cgaagccctc aaagaccgcc aaaacacaga caaaccataa
     1861 ggaacacaca tgacaccgct caaaatcgcc atcgccggcg caaacggccg tatgggacgc
     1921 gtattggttg aagccgtcaa caaccatccc gacaccgtcc tttctggtgc gcttgaacac
     1981 tcaggctcag aagccctcgg gctggacgca ggctacgccg tcggactcaa aaccggcatc
     2041 gccatttcag acgacgttga cgccgttctc gcacaaagcg acgtactcat cgacttcacc
     2101 cgccccgagc ccaccctcaa acacctgcaa aaatgcgttg aaaaacaagt caacatcatc
     2161 atcggcacaa caggcttcga cgatacgggc aaagccgcta tccacactgc cgccgaaaaa
     2221 acaggcatcg ttttcgccgc caacttcagc gtcggcgtca acctcacctt ccacatcctc
     2281 gacaccgtcg cacgcgtatt aaacgaaggc tacgacatcg aaatcattga aggccaccac
     2341 cgccacaaag tcgatgcccc cagcggcacc gcgttacgca tgggcgaagt catcgccggc
     2401 gcgctcggca gagacctcaa acaatgcgcc gtttacggcc gcgaaggcca caccggtccg
     2461 cgcgatccgt cgaccatcgg ctttgccacc gtccgcgcag gcgacatcgt cggcgaccac
     2521 accgccctct tcgccaccga cggcgagcgc gtggaaatca cccacaaggc cagcagccgc
     2581 atgacctttg ccgccggtgc cgtccgcgcc gcagtttggg tcaacggcaa aacgggtttg
     2641 tacgatatgc aggacgtact cgggctgaac aaccgttaac ccccatacaa aatgccgtct
     2701 gaaaagatat tgttcagacg gcattttgcc gacaggctcc gtatcggcat atcaatgttt
     2761 caacacacag gacgacacat aaagcgtcgc cctatgtgtt gccctgattc ggaaggggtt
     2821 acgcccctcc caaataaaat ctgattctac cgccccgaag gacagatgtc cgagtggcgg
     2881 ggtttcaacc gaaaaggaaa tacgataaag ttgcctgctc agcataacaa gctacggctc
     2941 gtttaattga aactctcctg atctaaaaat tctaactcta tttcccggca aactatatct
     3001 atactaaaac cggtcggaag atcataccta ctgcctatca cttccagcgc acctccataa
     3061 tccaagattt catcatcctg ctttaattct ccaagttcca atacattttt gatattttta
     3121 tcttggttct tcgtggcgta tccatagata aatttcagat agctgaactg cttgaattta
     3181 atttttaccc agtctgtata atttggtgta ggaatctctt gaggaaagtc cggataatcc
     3241 acattctgga cgatgatatg gaattcatcg tttttcaaag tatagtttaa tagagtgccc
     3301 tcatatatat acagaaattg ataatctttt tttggaatgg tataagttgt catattattt
     3361 taccttttca aaaacaatga gtttaagatt ttttgagtcc aagttggttc tactactctt
     3421 atgaaatttc agttgtttaa actcttcctt tattttaagg aatttttcaa ctagccgaga
     3481 acaaactgga gcaaaatgga cgattgagtt taggaatcaa cctgattcag tacatgggca
     3541 aagattagaa ttaaaattta gataaaggtt acgttatgaa tgtgaagata aaatataatc
     3601 ttttagaata ttgtgaatat ttaggtggta tttttggtgt ttataaaaat tatttaaata
     3661 tggatatcaa agatccaaat ttaaaaatta gattttttga ttttttggaa gaacttttat
     3721 cagatggtgt tattgaatta tgcgattatc gtgaaaatcc tccaaaaata ttaactggtt
     3781 cgccaaaaac acaggtagat gaattaagaa gaatttggcc ggatatggaa gagatgttgc
     3841 tatattttcc agataatcct tggttttatg tagaacattt ttggtgggga gcaacttgcc
     3901 ctattgaact gacacaattg cctaaaatag aaatttacga agaacaaatg aaacaaggta
     3961 agtaagcgta ttagtataag ctataatcat aaatcatata taacaaaatt aatcagttaa
     4021 ctgaccatta tattttaacc ttaatcccat tcgggccagt tctgtttcat ttggaattcc
     4081 tcttaaatac ttattgtttg atgtgccatt aatcacaatt tctccccctt tttttgtaat
     4141 acgtgctgct tcgggaaaat aatccattaa ccgctcaaca tcccgcctct tccaaaaccg
     4201 cgcgcgcggc caaatccgcc gtatcttttt tcaacagcag gtgcaacgcc ctgaaatcct
     4261 gttgcagcgc ggcaacctta tcggggtgtt cgtaccagtc cgccaacgcc gccgccagtt
     4321 tttccggttt tgcttcagat tgcaataatt ccggcacagc ctccttaccc aacaggatat
     4381 tcggcaggcc gacatgcggc actttgattt tgcgtttcac ataagcatag gtcagcggcg
     4441 aaatcttgta gctgatgacc atcggacgct tacacaacgc cacctccaaa gttgccgtac
     4501 cgctcgttac cagcaccgca tccgccgccc tgcacactgt ttcagactgt ctgtcgatta
     4561 ccgtcagcgg caatccggca aactccggcc gctgcaaaac ttccgccaaa cgccgcttcg
     4621 tcgcctccgt tgcggcaggc agcaggaagc gtgcggcggg atagcgttcc aacaacaata
     4681 atgccgtctg aaaaaacacc ggcgccatat agtcgatttc gctgacgcgg ctgccgggca
     4741 gcagggcgaa tacggggatg ccggcatcca cgcccaaagt ttgccgcgcc gtttcacggt
     4801 cgtcttccaa gggcataagc tgcgccatcg gatgaccgac aaactccgca cgtccgcccg
     4861 catcgagata aagctgcggc tccatcggga acaggcacaa cacgcggttg acctgatgca
     4921 cgattttgcc cacacgttcc cgccgccacg cccacaccga cgggctgaca taatgcacgg
     4981 tcggaatccc cgaccgtttc agcttttccg ccacacccaa attaaaatcg ggcgcatcga
     5041 taccgacaaa gacatcaggt ttcaacgaca gcaaatcccg taccagcccc ctgcgtatcc
     5101 gtaaaatttc cggcagccgc ctgaccactt cgacaaagcc gcgcaccgcc agccgctcct
     5161 gatcataaag gctctcgaaa ccttccgcct tcatcagttc gccgccgata ccgacaaaac
     5221 gcgcctgcgg acaacgcttg cggatggcgc gtatcaggtg cgcccccaat aggtcgcccg
     5281 acgcttcgcc gacactgacg gcaatcaaag ggcttttttt atcagccata ttcgtctgtc
     5341 cccacatact ttcacgtccc gatgccgtct gaaggcttca gacggcatcg gggttcacat
     5401 tttcgtcccg ttttccaaca tcaccgcaaa ctgcgccaag tccggcggca gctccgcctt
     5461 caacaccagc ggctcgcccg tgagcggatg gttcaagtgc agctcggacg cgtgcaaaaa
     5521 catccgcttc aaacccaact tctgcaaacg acggttcgcc tgataatcgc cgtagcgttc
     5581 gtcgcccgca atcggacagc cttgagattg caggtggacg cggatttggt gcgtgcgccc
     5641 cgttttcaac gtcgcccgca ccaaagtcag gtgcgacagc ccgacaccgt gcaaaatgcc
     5701 gtctgaaaaa cggcttaaca cacggaacac cgtatgcgcc gactgcccgt cctcgctgac
     5761 gcgtaccatc ttttcgcctt gtgcgccggt atatttgaac aggggcagtt tgacatggaa
     5821 attgtcgtcc ggcagtttgc ccacccccag cgcaaggtag atttttttgg ggtggtcgtt
     5881 acggatggct tcgtgaagtt tgacgagcgc gctgcgtttc ttcgccacca tcaacaagcc
     5941 gctcgtatcc ttgtccaaac ggtggacgag ttcgagataa cgcgcctccg gacgggcgcg
     6001 gcgcaactgt tcgataacgc cgaaactcac gccgctgccg ccgtggacgg caacgccgga
     6061 cggtttgtcg atgaccaaaa gcgcatcgtc ttcgtaaaca acgtcaaacg cacgcgccgg
     6121 tacggcggca cgcctttcag acggcatttc cttctccgcc acgcgcacag gcggaatccg
     6181 caccgtatcc ccctccgcaa tacggctgtc gggtttgcag cgtttcttgt tcaaccgcac
     6241 ctcgccggcg cggataatgc ggtggatatg gctcttggga acacccttga ggatttttat
     6301 cagatagtta tcaaggcgtt gacccgcctc atgttcggca acccctatca agctgaccga
     6361 atctttgctt atttcgtgcg ttttcatcta taatccgaac atccgtttca gcaaaaagcg
     6421 cgcccgccgc gcccccctga aacggtttac tttaaaacgg aattttatat taaaaacgca
     6481 ctccgcaaag cattttccct gcgcctgttc ccagccggca ggcgcagaac gtaatcaagt
     6541 ttgaattgat tttgccgttt cggcgcggaa gtaagacggc agccgggccc caagtcccaa
     6601 acgcaaaccg cccaaaacac aggcatcacg ataacaagaa gaagccggcc cccatttttc
     6661 cgaccgcagc gttcggaacg cggcagacaa accgccgcat cgatctttat agccttctgt
     6721 atccgaccct tccgcacacg gttctccgga acgtgcatcc ctgcggattt tcaactcaaa
     6781 aactgattac cggttttatc cgaagacatc ggaaaaccca gccttacgga atgccgaacc
     6841 ggaccggcgc gcccgaatgc cgcccgtcca cgaggtgatc atgaaaagaa tgttattcaa
     6901 cgcaacgcag gccgaagagc tgcgcgttgc catcgtcgac ggacaaaacc tgctggattt
     6961 ggacatcgaa acgctgggca aagaacagcg caaaggcaat atctacaaag gcatcattac
     7021 ccgcatcgag ccgtcgctgg aagcgtgttt cgtggattac ggaaccgacc gccacggttt
     7081 cttgccgttt aaagaagtct cccgctcata cttccaagac tacgaaggcg gacgcgcgcg
     7141 catccaagac gtgctcaaag aaggcatgga agtcatcgtc caagtcgaaa aagacgagcg
     7201 cggcaacaaa ggcgcggcgc tgaccacctt catcagcctc gccggacgct atctggtatt
     7261 gatgccgaac aacccgcgcg gcggcggcgt atcccgccgt atcgaaggcg aagaacgtca
     7321 agaactcaaa gccgctatgg cgcaactcga cattccgaac ggcatgagca tcatcgcccg
     7381 taccgccggc atcggccgca gcgcggaaga gttggaatgg gatttgaact atctcaaaca
     7441 actctggcag gcgattgaag aagcaggaaa agcgcatcac gacccttacc tgctctttat
     7501 ggaaagctcg ctgctcatcc gcgccatccg cgactatttc cgccccgaca tcggcgaaat
     7561 tctggtggac aatcaagaag tttacgacca agttgccgag ttcatgagct acgtcatgcc
     7621 gggcaatata ggccgtctga aactctacga agaccacacg ccgctgtttt cccgcttcca
     7681 aatcgaacac caaatcgaaa gcgcgttttc gcgcagcgtc agcctgccct ccggcggcgc
     7741 gatcgtcatc gaccataccg aagccctcgt ctccatcgac gtgaactccg cacgcgccac
     7801 tcgcggcgca gacattgaag acaccgcgtt caaaaccaat atggaagccg ccgaagaagt
     7861 cgcccgacaa atgcgcctgc gcgacttggg cggcttggtc gtcatcgact tcatcgacat
     7921 ggaaaacccc aaacaccagc gcgatgttga aaacgtcctg cgcgacgcgc tcaaaaaaga
     7981 ccgcgcccgc gtgcaaatgg gcaaactttc acgtttcgga cttttggaat tgagccgcca
     8041 acgcctgaaa ccggctttgg gcgaaagcag ccatgttgcg tgtccgcgct gcgccggcac
     8101 cggcgtgatt cggggcatcg aatccaccgc cctgcacgtt ttacgcatca ttcaagaaga
     8161 agcgatgaag gacaacaccg gagaagtgcg cgcacaagtg cccgtcgatg tcgccacctt
     8221 cctgctgaac gaaaaacgcg ccgagctgtt tgcgatggaa gagcgtttgg atgtcaacgt
     8281 cgtcctgatt ccgaacatcc acctcgaaaa tccgcactac gaaatcaacc gcatccgcac
     8341 cgacgacgta gaagaagacg gcgaaccgag ctacaaacgc gtcgccgagc cggaagaaga
     8401 cgaatccgcc aaaccgttcg gcggcgaaaa agccaaagcc gcccgtcccg aacccgccgt
     8461 caaaggcgtg cgccacacca gccccgcccc gactgccgcc cccgagaaaa aaacctcctg
     8521 gtgggacagc ttcaaagctt ggctcaaacg cattttcggc ggcagcgaaa cccaagccgc
     8581 acccgctgcc gaaacctccg aaaaacgcag cacggcaaac cgcagcggca gccgcgccaa
     8641 caaccgccgc caaaacccgc gccgcagcaa acgcgaaggc agcaaagtag aagtccgcga
     8701 agtggcaggc aaaactgcca gacaggaagc gcgtgccgac acagccgaaa cgcgcaacaa
     8761 cggcaaccgc cgccgcaacg aacgcggcga ccgtgccgcc gaacgtgcca acgaagcgga
     8821 aatccaaagc cgcaacgtac agcctgccgc aaccgttgca gatgccgcac cgtccgaaac
     8881 cgaagtgcaa accggaaaac gccgccgcaa cggcagccgc agcgaacgca gccaaaccgc
     8941 gccggaaacc gccgccgttg ccgaaacaac cgttcagaca gcggaaaaca cgccgtccga
     9001 accgcatacc gcagaagaca aaggcagcaa gcccaaatcc gaacgcaacc gccgcgaacg
     9061 cgacagccga gatgccaaag aacgccgcga gcgcaacaat cagcgcgacc gccgtcaaaa
     9121 cggcaaaaaa cgcaatattc cgtctgccgc caaaatcgag cagtacctga atattcacga
     9181 caccgccgac aaagtccgtt ccgccgccgc gcacgttttc ggcgaaaccg acgcaaacgc
     9241 gccgattacc gtcagcattg ccgatccggt tgcagaaagg gatcttccga cagcatctcc
     9301 cgccgtttca aacggcgacg caccggttta tgatacggcg gaaaaaatcc gccgtgccac
     9361 cgccgccatc ctgcccgaag gcgcgacacc gaaagccgaa gcacaggaaa tgccgtctga
     9421 aaccgcaacc tttacggctg cggcggaaca ggcacgggaa accgcacaaa ccggcggact
     9481 cgtcctgatc gaaaccgacc ctgccgcatt gaaggcatgg gcggcacaac ccgaagtcca
     9541 agccggacgc ggtttgcgcc gttccgaaca gcccaagccg tctgaagccg caaccgtccc
     9601 tgccgaagaa atgatccaag tcgaaacccg gcaaggctga accgacggcg gcaaaaagag
     9661 gttctgttcc gcagaacctc ttttttacat gggttcggat acctgcaatg ccgtctgaaa
     9721 cttcgccatt cccgtgatta ccgaaacatt ccg
//
LOCUS       DAWKOG010000067         9698 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.067, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000067 DAWKOG010000000
VERSION     DAWKOG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9698
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.067"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(32..244)
                     /locus_tag="WJK66_001190"
     CDS             complement(32..244)
                     /locus_tag="WJK66_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7578908.1"
                     /translation="MNHAETEPRDGLYVLRIGNDLFVKRVQRIPGKLLVTSENPRYAP
                     FEIDLSNTQDDIAIIGRVEWYGRSID"
     gene            complement(411..1124)
                     /locus_tag="WJK66_001191"
     CDS             complement(411..1124)
                     /locus_tag="WJK66_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEZ7578909.1"
                     /translation="MRLYESGQVNIPADFLLAAVQLGVDIQFVFTGIHSANLNAVSDD
                     LNKKLDDAIGKSNVENQINGNVSNAVIASSSSVVHQINTQNHVIRTRVDSKPGKEHIT
                     LEQASKLQQLVKQVAAAEEIAKRSPKSIRAIWASLNAHCKVPSYKLIALSDYDKAETY
                     LRKWLGRLSNTATSKNNDPDWRKKKYAYIKLNVKQLELEDWLKSYLEKNFAVESLTEL
                     SNDDLQKTYAAVSTKKRKK"
     gene            1357..1608
                     /locus_tag="WJK66_001192"
     CDS             1357..1608
                     /locus_tag="WJK66_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7578910.1"
                     /translation="MQANEIYKSLEEKNISARMLASALGVTNQSVSEVIRNGRGSKRI
                     AEAIAKVIQKDLVEVFPHYKKTDCRDKKIAELKEMLGVG"
     gene            1610..3587
                     /locus_tag="WJK66_001193"
                     /pseudo
     CDS             1610..3587
                     /locus_tag="WJK66_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3654..3731)
                     /locus_tag="WJK66_001194"
                     /pseudo
     CDS             complement(3654..3731)
                     /locus_tag="WJK66_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            3802..4716
                     /locus_tag="WJK66_001195"
     CDS             3802..4716
                     /locus_tag="WJK66_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEZ7578911.1"
                     /translation="MKQINQALQQKLVEFKEKSGMNQTQLARGIDTSPASISMYLNGT
                     YADKGGNYETIETKIEAFLEMQDSKARREELVLGFVSTKTTRRIAEAMRDAHEGGEIV
                     VIYGQAGLGKTQAVKNYCEKNPAAILIEANPSFTALVLMRKLATAAKVSAMGSLNDLF
                     ESVSDRLRDSGRLIVVDEAENLPLRALEIIRRLHDETGCGLVLSGMPRLVANLRGKHG
                     ELVQLYSRVSLPLNLGESLPDEELFEIAKAALPDADEETLLELVKHSNGNTRRMSKLM
                     RGAVRTANKNGIKMQAGIVKKYSSLIIR"
     gene            4807..5073
                     /locus_tag="WJK66_001196"
     CDS             4807..5073
                     /locus_tag="WJK66_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578912.1"
                     /translation="MQVLKKVDWKMFVARSFWRCVPVGLTAGVWCFVAGMALHSCAKE
                     PEPVAKELTKVEKMERQADLEVKVWEEQYESMSVEEKMKGIVYE"
     gene            5182..5355
                     /locus_tag="WJK66_001197"
     CDS             5182..5355
                     /locus_tag="WJK66_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578913.1"
                     /translation="MSFGRRNTDWQAWGQHRRRATARMAQKSREREIEEYQAQFEDKD
                     GKGRLKTGGNKDE"
     gene            5348..5515
                     /locus_tag="WJK66_001198"
     CDS             5348..5515
                     /locus_tag="WJK66_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578914.1"
                     /translation="MNEKDLIEWLEDRGELMVMKKDGEGFVIAARAPDGMWKTAEAET
                     LAQAITLWEEV"
     gene            5579..5806
                     /locus_tag="WJK66_001199"
     CDS             5579..5806
                     /locus_tag="WJK66_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578915.1"
                     /translation="MKVYVFKISNENGKLKIELPEIPMGKQIDEVDLIAGLTTEFIAS
                     MLRDAQKDRRKFVIDASNQLAAIQTYQKIFN"
     gene            5821..6339
                     /locus_tag="WJK66_001200"
     CDS             5821..6339
                     /locus_tag="WJK66_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HEZ7578916.1"
                     /translation="MAKTRIKQPAIEAAQDKTEVTAFIRQIGDLQREVKRLETEAGDK
                     KAAIEEEYAAKAAPMCAEIMSLTERVAAYCEAHKDELTENGKTKTVDFTTGLIKWRIR
                     PPSVKVTGVAAVLAWLSEKSAFAEFVRTKKEIDKDAILNQKERFSDGQVPGIKIVSGV
                     EDFVIEPSEQIL"
     gene            complement(6499..6714)
                     /locus_tag="WJK66_001201"
     CDS             complement(6499..6714)
                     /locus_tag="WJK66_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578917.1"
                     /translation="MNTTYTLTFDQDSLKALNLLVFTLNHLKVLDMDMEGIEDGLSAV
                     IETASEKADKLSAAFYNAVYEQEEKAA"
     gene            complement(6718..7326)
                     /locus_tag="WJK66_001202"
     CDS             complement(6718..7326)
                     /locus_tag="WJK66_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HEZ7578918.1"
                     /translation="MKDNFTMNTQLIPTVSGQLDNQTQALVDAHDLHKFLGVETPFSK
                     WIQRRIEEYGFTQALDFIGVDKIVRTEAGFFGQRDKTVQGYYLSLDMAKELCMVERND
                     KGRQARRYFIEMEKQAKALPDAVLYRIDALEDAYFQAAPEMLALLRYRSMGLNLTEIG
                     KLLDMNPGAVSYRLKKLNDLGFLEYVPKPKMVVQQQSLGLEG"
     gene            complement(7458..7658)
                     /locus_tag="WJK66_001203"
     CDS             complement(7458..7658)
                     /locus_tag="WJK66_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578919.1"
                     /translation="MDSIKKAQEKYALKRVVKTVSFNSEKELDLIKFSENLDFSNWVK
                     SKIRGDMESEKFKKKAKKNIDK"
     gene            complement(7859..8044)
                     /locus_tag="WJK66_001204"
     CDS             complement(7859..8044)
                     /locus_tag="WJK66_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578920.1"
                     /translation="MPDEKTLKAKQAEYQRKYDQKRLMKTVSFHLEREKELVDFMQKE
                     IPDFSNWVKSVMKEKMK"
     gene            complement(8317..8484)
                     /locus_tag="WJK66_001205"
     CDS             complement(8317..8484)
                     /locus_tag="WJK66_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578921.1"
                     /translation="MVDEKLAENRKRYEQKRVIKKVSFNAETEKELLEYAQNIDFSQW
                     VKSIIKEKIKK"
     gene            8721..8963
                     /locus_tag="WJK66_001206"
     CDS             8721..8963
                     /locus_tag="WJK66_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578922.1"
                     /translation="MDTLKVNPFPNKIKEKCRLKTARMLFQTAFFVVGHQCPAICAAL
                     RLSRYGILVCIFRRNLPYIVLKYYTVVLYGFDFLEK"
     gene            9007..9198
                     /locus_tag="WJK66_001207"
     CDS             9007..9198
                     /locus_tag="WJK66_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578923.1"
                     /translation="MNTVKEYTAVRERLLNAADYLEEVRKDRKTGNIASVEFVPPKIG
                     ARGYGKFKVRYKTLVAVDL"
     gene            9195..9611
                     /locus_tag="WJK66_001208"
     CDS             9195..9611
                     /locus_tag="WJK66_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578924.1"
                     /translation="MSGEKSENLPADEQQKMLQEFLELQKQDIAVKQKELESRKDEIQ
                     SHERIALATIEAQKQGEQQHGEIFKEVHKRRLNAVIAISFLIAAVLITALWRDKDSVA
                     IEIVKIGGAVALGYFAGINRGKAQTLEKQRREKDSD"
BASE COUNT         2676 a         1951 c         2502 g         2569 t
ORIGIN      
        1 aaaactgttt taagaggttt ttaaaatgga tttaatcaat actccggcca taccactcaa
       61 cacggcctat gatggcgatg tcgtcttggg tattactcaa atctatttca aacggtgcgt
      121 aacgtggatt ttcagacgtt acaagcagtt tgcccggtat acgttgcaca cgtttgacaa
      181 agaggtcatt gcctatacgc aagacatata ggccgtcgcg cggctcggtt tcggcgtggt
      241 tgattagaat gtttataaaa ggctttaact agaaactgaa catggaactt gtataatagt
      301 tccatgtttg aaaaaatctt ttaatatcat acatctaaaa aaacaaaaca tctgaagacc
      361 ataagctaaa catgaaactt gaatttaaag gcggagtttc atgtttaagc ctattttttc
      421 cgttttttag tcgatactgc tgcatatgtt ttttgcaaat cgtcattact taattcagtc
      481 aggctttcta ctgcgaaatt tttttcgagg taacttttca accaatcttc aagctcaagt
      541 tgttttacat ttaatttgat ataagcgtat ttcttttttc gccagtcggg atcgttgttc
      601 tttgatgttg ctgtattgct caacctaccc agccattttc tgagataagt ttctgctttg
      661 tcgtaatcgc tcaatgcgat tagtttgtaa cttggaactt tacagtgagc attcagacta
      721 gcccaaattg cacggattga ttttggcgac cgtttggcaa tctcttctgc tgcggcaact
      781 tgtttaacaa gttgttgcag ctttgaagct tgctcaagcg ttatatgttc cttgccgggt
      841 tttgaatcga cacgggttct gatgacgtga ttttgggtat taatttgatg aacaaccgaa
      901 ctagatgagg cgataacagc gttactgacg ttgccgttaa tctgattctc aacgtttgat
      961 ttgccaatag cgtcgtccaa ttttttatta aggtcgtctg aaacagcatt taaatttgcc
     1021 gaatgaatgc ctgtaaagac aaattgaata tccaccccta attggacagc agcaagtaaa
     1081 aagtccgcag gaatgttgac ctgcccgctt tcatacagtc tcaaactttc tgcactgcat
     1141 ccagtttgct cagcaaaatt ctttcgagag tatccaagtc ggcttctctc tttaactaaa
     1201 ttcaatgcta aatcaatgcg ttccataaaa caccgcaaaa tagtacccaa tatatttggt
     1261 ataccaaata tattgggtat atcatccagg taacagttaa ggcaggcatt aaagcctgta
     1321 aggtaagtaa gtaatggatt ctatcatgga ggcattatgc aagcgaacga aatctataaa
     1381 tccttagaag aaaaaaatat atcggcaaga atgttggctt cggctttagg ggtaactaac
     1441 caatctgttt ctgaggtcat aagaaatgga cgaggaagca aaagaattgc ggaggcaatc
     1501 gcgaaagtga ttcaaaaaga tttagttgaa gtttttccgc attacaagaa aacagattgt
     1561 cgagacaaaa agatagctga attgaaagag atgctcggag tgggttgaaa tggaatatat
     1621 ggatctcaaa agcctgctta attttgggtt gccaaatatg ccaagaacag taatagggct
     1681 gagaaaaaaa agcgcaaagg gatggttggc aaacgagaag gcgtcaaggt aagggtggtg
     1741 gtgttgaata tgccctacct attgaaatcc gagcagccat catgaaacgg cagtcggacg
     1801 agctggcgga aaagatgccg aaaatgttgc cccaagtcag accggggacg gcgatgtcgg
     1861 ctcaggcact ggctgaagcg gccaagctgt tgaacgagaa acaacggtcg gtggcggatg
     1921 cgcgatgtgc ggtggtggcg gcggtattgg ggattaaata ccaatacggt tgctctgcaa
     1981 aggctgcggt ggctcagttt ttgggcttgc tggcagaaag taaattggac gcggtcacgc
     2041 ttgggaactt ggaaaaggcc aatgaccgca gccggacggc aaaggttggc gaacgtactt
     2101 tagacggctg gatttctgct tatttgaaag cggaaaacgc gacggagcgg ttggttgctt
     2161 tggctccgaa gacgacgaag gcggtcaagc cgattgagag ctacggttgg ttgccgatgt
     2221 ttatgcagtt tcacaatatc ccttcggctc cgaaactggc gcacagctac cgccggtttg
     2281 tgcagtgggc ggaagcggaa aatatgccgg tcaatgatgt gcctaacttg agtatggtgc
     2341 ggcgcgtttg ggacaagctc ccgctgatta tgcaggagcg cggcaggaaa acgggggcgg
     2401 cttataaatc gctgttgcct tatgtgaaac gtgattgggg ggctttgaag ccgaacgatg
     2461 tttggatcgg cgacggccat agctttaagg caaaggtggc gcatccggta cacggcaggc
     2521 cgtttaagcc ggaagtgacg gtgattattg atggttgtac gcggtttgtg gtcggttttt
     2581 cggtgtcgtt ggctgaaagt tgtgtggcgg tatcggacgc tctgcgtatc ggggtcaagc
     2641 actttggttt gccgattatc tactactcgg ataacggcgg cggccaaacc ggcaagacga
     2701 tagaccatga aatcacgggt attacgtccc gattgggtat ccgccatgaa acgggtatcg
     2761 cgggcaaccc gcaagggcgc ggcatcatcg aacgatggtg gaaagacaat ctgattgaga
     2821 tggcgcgaca gtatgagacg tttgcgggcg cggggatgga cagcagcacg aagaacctga
     2881 tgtaccgcaa gatggaaagt gcttttaatg ctttggaaaa aggcaaggag ttgacggtag
     2941 aacagcaaaa atatttgaaa aaactgccga gctggtcgcg ttttatcgcg gatgtggcca
     3001 agtgtatcga cgaatacaac aaccgcccgc acggcgagct gccccgacat cctgacggcg
     3061 ggcattattc gccgaaggct tatcgggaaa tgaggctgga acaggacggt atcgcgccgg
     3121 atatgttgtc ggcggaagag ctggcgacga tgtttatgcc gcaggaggtg cgaaaagtac
     3181 agcgcggttg gctggatttg ttcaacaact cttatttctc aaccgagctg gcggagtatc
     3241 acaaggacga ggtacgggtc agctacgatt tgagcgatgc gtcggcggtc aatgtgtttg
     3301 atatggacgg caagtttatc actaaggcgc aggccaacgg caatacccgc gaggctttcc
     3361 cgacggctcg tatcgaccaa ctggcggaaa accgccgaaa aggcaaaatc aagcgggcgg
     3421 aaaatgcaat caagctcgca aatgcggaag tcaatccggc actggaacag gctgcggttt
     3481 gggacgagct gggacatttg ggcggaaacg tcatcgaggc ggagtatgcg gtattgccga
     3541 aaacgggcac ggacgacggg attttcttgt ttgaggcgga tatgtaaagg aaaacatgat
     3601 ggaaaaacag aaaaatgcgg cgttttcggc cgagcttgtt gaaaaattga aactcaagcg
     3661 agcccttggg cggattcaac gatttcaagc aaagattcaa ggtgttcccg ctgaacggaa
     3721 tcaggctcaa atttctaaat cgggaaactc tttcatttca gacggccttt aaaggttgtt
     3781 taaaactcaa ggatattaaa aatgaaacaa attaatcaag cattgcaaca aaaactggtt
     3841 gaatttaaag aaaaatcggg tatgaaccaa acccaactgg cacgcggtat cgatacttca
     3901 ccggcctcca tcagtatgta tctgaacggc acttatgccg ataaaggcgg caattatgaa
     3961 accatcgaga cgaaaatcga ggcgtttttg gagatgcagg acagtaaagc gcgacgtgaa
     4021 gagctggtgt tgggatttgt atcgactaag acgacacgcc gtattgcgga agcgatgcgc
     4081 gatgcgcacg aaggcggcga aatagtggtg atctacggtc aggccggttt gggcaagact
     4141 caggcggtca aaaactactg cgagaaaaac cctgcggcca tcttgattga ggctaatccg
     4201 agctttacgg ctttggtcct gatgcgcaag ttggcgacgg cggcgaaggt atcggcgatg
     4261 ggcagcctga atgatttgtt tgaatcggta tcggacagat tgcgcgattc gggccgtctg
     4321 attgtggtcg atgaagcgga aaacctgccg ttacgcgccc ttgagattat ccgccgactg
     4381 catgatgaaa cgggctgcgg tttggtgttg agcggtatgc cccgattggt ggcgaatttg
     4441 cgcggtaagc atggcgagct ggtacagctt tacagccgcg tgtcgcttcc gctgaatttg
     4501 ggcgaatctt tgccggatga agaactcttt gagattgcga aagcggcttt gcctgatgcg
     4561 gacgaggaga cgctcttgga actggttaaa catagtaacg gcaatacgcg ccggatgagc
     4621 aaattgatgc gcggcgcggt acgcacggcg aacaagaacg gtatcaagat gcaggccggt
     4681 atcgttaaaa aatacagctc cctgattatc cgatagggcc gtctgaaacg gtaagtctct
     4741 gacagggcta tatatttttt taccctatga ttttaataag ttgttgtttt aaaaggaaaa
     4801 cgcaaaatgc aagttttgaa gaaagttgat tggaagatgt ttgtggcacg ctctttttgg
     4861 cggtgtgtgc cggttggttt gacggcgggc gtgtggtgtt ttgtggcggg gatggcgttg
     4921 cattcctgcg cgaaagaacc tgaaccggtt gcgaaagagc tgacgaaggt cgagaagatg
     4981 gaaagacagg cggatttgga ggtcaaggta tgggaagagc aatacgagtc aatgagcgtc
     5041 gaggaaaaaa tgaaagggat tgtttatgag tgataagcca attttattga gtcctgcggc
     5101 gaaaaaagag gctttggatc gggcggtaaa ggaaatccgt gcaaaatatg gcgataaggc
     5161 gattgtgaaa ggatgtgtga aatgagtttc ggacgacgta atacggattg gcaggcttgg
     5221 ggacaacacc gcaggcgtgc gacggcgcga atggcgcaaa aaagccggga gcgcgaaatc
     5281 gaggaatatc aggcgcagtt tgaagataag gacggcaaag gtcgtctgaa aacgggagga
     5341 aacaaagatg aatgaaaaag atttgatcga atggctcgaa gaccgtggcg agctgatggt
     5401 catgaagaag gacggcgaag gctttgtgat tgcagcgcgt gcgccggacg ggatgtggaa
     5461 gactgccgag gcggaaactt tggctcaggc gataacttta tgggaggaag tgtgatgttt
     5521 aagtttttga gaaaccgcgc gaaagttgaa gcaattttaa acaagaaagg aaagtgaaat
     5581 gaaagtgtat gtttttaaaa ttagtaatga aaacggtaag ttaaagatag aactccccga
     5641 aatcccaatg ggtaagcaaa ttgacgaagt tgatttgatt gctgggctaa ccacggaatt
     5701 tattgcaagt atgttacgtg atgcccaaaa agatcgtcgc aaattcgtaa tcgacgcatc
     5761 aaatcaattg gctgcaatcc agacatatca aaaaatcttc aattaaggaa ggaagagaaa
     5821 atggctaaaa cccgaatcaa acagcccgcc atcgaagcgg cacaagacaa aacggaagtt
     5881 actgcattta tccgccaaat cggcgacttg cagcgcgaag tcaaacgcct ggaaaccgaa
     5941 gccggagaca aaaaagcggc tatcgaagaa gaatatgccg ccaaagccgc gccgatgtgt
     6001 gccgaaatca tgagcctgac cgaacgtgtg gccgcctact gcgaagcgca taaggacgag
     6061 ctgacggaaa atggtaaaac caaaaccgtg gactttacta ccggcctgat taaatggcgc
     6121 attcgtccgc catccgtcaa ggtaacgggc gtggccgccg tcttggcgtg gctctcggaa
     6181 aaatccgcct ttgccgagtt tgtccgcact aaaaaggaaa tcgacaaaga cgccatcctg
     6241 aatcaaaaag agcgtttttc agacggccaa gtgccgggga ttaagattgt gagcggcgtg
     6301 gaggattttg tgattgagcc gagcgaacaa attctataaa atcaacagcc tgatttcaat
     6361 tcagtgtgtt gaaacgtgat tgagcctacg gagcaggagc ttgcctagcc ggttcaaata
     6421 ggctttaacc catgattaaa ggccgtctga aatggggttt aaaacctgtt tcagacggcc
     6481 ttttttcata ccctagattc aggctgcttt ctcttcctgc tcgtacacgg cgttgtagaa
     6541 agcggcggac agcttgtcgg ctttctcgga ggcggtttca atcacggcag acaaaccgtc
     6601 ttcgattcct tccatgtcca tatccaaaac tttcagatgg ttgagcgtga acaccagcag
     6661 attcagggct ttgaggctgt cttggtcgaa agtcagagta taggtggtat tcatggctta
     6721 accctccaat cccaaagact gttgttgcac caccattttg ggcttgggga catattccaa
     6781 gaaacccaaa tcgttgagtt ttttgagacg gtaggaaacc gcgccggggt tcatgtccaa
     6841 gagtttgcct atttcggtca ggttcaagcc catactgcgg tagcgcagca gcgcgagcat
     6901 ttcgggcgcg gcttggaaat aggcgtcttc caatgcatcg atgcggtata gcacggcatc
     6961 aggcagggct tttgcctgtt tctccatttc gataaaatat cggcgggctt ggcgtccttt
     7021 gtcgttgcgc tccaccatgc acagctcttt tgccatgtcg agggataaat aatacccctg
     7081 aacagtttta tcacgctgtc caaagaaacc tgcttcggtg cggacaattt tgtccacgcc
     7141 gataaaatca agggcttgtg taaatccgta ttcttcaatg cgacgctgaa tccatttaga
     7201 aaatggtgtt tccacaccta agaacttatg caggtcatga gcatcaacca gtgcttgtgt
     7261 ttgattgtcc aattgacctg aaacagttgg gatgagttgg gtattcatgg taaaattatc
     7321 tttcatttca aggttgagat gacaaggaaa agaacgctct aacgttgctt ttccactatc
     7381 cccgataagc tgtcacttat cggggatttc catatcttgt gttgcacaag atatggaaat
     7441 tttaaattat ggtaattcta tttgtcaata ttcttttttg ctttcttttt gaatttttcc
     7501 gattccatat ctcctctaat tttcgatttt acccagtttg aaaaatctaa attttcagaa
     7561 aatttaatta aatcaagttc tttttcggaa ttgaacgata ctgttttcac tactcgcttg
     7621 agtgcgtatt tttcttgagc ctttttaatc gagtccataa tcttccttta aataggtttt
     7681 aaaacacgat tatggtaata ctaaaaacca ttttaatcaa gatgtattac aacaaaaagc
     7741 cgtctgaaat ggggtttaaa acctgtttca gacggccttt tttcatgccc tacataaaga
     7801 gagttgctgc tctgctttcc acctgttccg ccgatgtttt cccatcggcg gaattttctt
     7861 atttcatttt ttccttcatg actgatttaa cccagtttga aaagtcggga atttcttttt
     7921 gcataaaatc caccaactct ttctctcttt ccaaatggaa agaaactgtt ttcatcagcc
     7981 gcttttggtc atactttcgc tgatattcag cttgttttgc ttttagcgtt ttttcatcag
     8041 gcataggcaa tccttgattt ttaaaatcgt tgtagtaaaa tggcttgcag aaaggttggc
     8101 gaaaggggcg cggtgttctg ccaccccttg tgtttaatca acttgcttag taagccggac
     8161 tacagcttaa aagcagaatg atgaaaaaaa caactttcgc cagttttgtc atttccacaa
     8221 gcctcctttc tatccgataa gcccccgaag ttgccgcttc ggggctttgc tttatcagat
     8281 aggcgtatta tctagtattt ctagaattta gtcaagctat tttttgattt tttctttgat
     8341 gattgattta acccattggg aaaaatcgat attttgggca tattccaaaa gttctttttc
     8401 cgtttcggca ttgaacgaga ctttcttaat cacccgtttc tgctcgtaac gctttcggtt
     8461 ttctgccagt ttttcatcaa ccataggcaa actccttgat tttttaaacc gtattttgta
     8521 agattgggac tggggacagc ggcgaccgcc cccagctggt cagtctttag taggcattag
     8581 cacttgccaa catgaaaact gcgaccaaga tgagaatttg aatagtgcgt ttattcatct
     8641 tctttctccc agttgagccc cgcttcggtg gggcttttcc cgtatcggcc ctcactacgc
     8701 cgatgaattg aattataaac gtggacacgt taaaagtcaa ccctttcccc aacaaaatca
     8761 aagaaaaatg ccgtctgaaa acagctcgaa tgctgtttca gacggcattt tttgttgtcg
     8821 ggcatcaatg ccctgccatt tgtgcggcgt tgcggttatc aagatatggt attttagtgt
     8881 gtatttttag aagaaattta ccatatatag tattaaaata ttatacggtt gttttatatg
     8941 gatttgattt tcttgaaaaa taaatcccat atgctaatat ttgcactatt tgggcgaggt
     9001 tgaaaaatga ataccgtcaa agaatatacg gcagtccgcg aacggctact caatgctgcc
     9061 gattatttgg aagaagtccg taaagaccgt aagacgggca atattgcgtc cgtggaattt
     9121 gtgccgccga aaataggggc tcggggttac ggcaaattca aagtccgtta caaaacctta
     9181 gtggcggttg atttatgagc ggggaaaaat ccgagaatct gccggccgat gagcagcaga
     9241 aaatgcttca ggagttttta gaacttcaaa aacaggatat tgcagtcaaa cagaaggagt
     9301 tggaaagccg taaggacgaa atccaatctc acgaacgcat tgcactggct accatcgaag
     9361 cgcaaaaaca gggtgaacag caacatggtg agattttcaa agaggtacac aaacgccgtc
     9421 tgaatgccgt gattgctatt tcttttctga ttgccgccgt gctgattact gcgttatggc
     9481 gcgataagga cagcgtcgct attgaaattg tcaaaatcgg cggggcggta gctttgggat
     9541 attttgcagg aatcaacaga ggtaaagcgc aaaccttgga aaagcaacgg cgcgaaaaag
     9601 acagtgatta gattgtagta aaaaaggtcg tctgaaacgt tttggcgaaa ctcgcaaagc
     9661 tcgttttcag acggcatttt ttatgtctgt ccgtttcg
//
LOCUS       DAWKOG010000068         9465 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.068, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000068 DAWKOG010000000
VERSION     DAWKOG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9465
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.068"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            214..993
                     /gene="pgeF"
                     /locus_tag="WJK66_001209"
     CDS             214..993
                     /gene="pgeF"
                     /locus_tag="WJK66_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEZ7578925.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDNPEAVRRNREIVQQQVGLPVAYLNQIHSTVVVNAAEALGGTPDADAS
                     VDDTGKVACAVMTADCLPVLFCDRAGTAVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            1045..1524
                     /locus_tag="WJK66_001210"
     CDS             1045..1524
                     /locus_tag="WJK66_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578926.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            1526..2524
                     /locus_tag="WJK66_001211"
     CDS             1526..2524
                     /locus_tag="WJK66_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEZ7578927.1"
                     /translation="MAAHIGHIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIG
                     ADALALFAERVEGNLLAARQEIDKLGLLYPKGHTVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVLRVCRLLDGLREEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSVQSVRNSLR
                     LWGEKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEDGDAWTVFKRLVVSLAE"
     gene            2645..3064
                     /locus_tag="WJK66_001212"
     CDS             2645..3064
                     /locus_tag="WJK66_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578928.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            3140..3655
                     /locus_tag="WJK66_001213"
     CDS             3140..3655
                     /locus_tag="WJK66_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ7578929.1"
                     /translation="MMTLKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGSWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAVGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(3739..4623)
                     /locus_tag="WJK66_001214"
     CDS             complement(3739..4623)
                     /locus_tag="WJK66_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEZ7578930.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDIRAVNHRFLDIQIRM
                     PDDLRHLESGIREKISSHLARGKVECKIQIQDTENGNQSLELNRDLVGQLAEINKDLR
                     KHHDLAKLGVADILRFPGVLASQRENTDALAKVIADLTDSALKDFTAARRREGKKLGE
                     HLLQRLESMEEIIGALSEIFPALVEAHKEKIRARLAEAVGSIDNDRLQQEFALFVQKS
                     DVDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIAAECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            complement(5107..5970)
                     /gene="rpoH"
                     /locus_tag="WJK66_001215"
     CDS             complement(5107..5970)
                     /gene="rpoH"
                     /locus_tag="WJK66_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEZ7578931.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(6234..7772)
                     /gene="lnt"
                     /locus_tag="WJK66_001216"
     CDS             complement(6234..7772)
                     /gene="lnt"
                     /locus_tag="WJK66_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEZ7578932.1"
                     /translation="MVSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFGLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASDNTARSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMNMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            complement(7841..8209)
                     /locus_tag="WJK66_001217"
     CDS             complement(7841..8209)
                     /locus_tag="WJK66_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HEZ7578933.1"
                     /translation="MAAIGIGMTVLVYGIVAVIVKLDDLGMLLMRRPQTFSRSLGQML
                     TAFMPCFMRGLSVVGTLAMFLIGGVLVAHNLGLLHDFLHAQHWDAGWAEYFANLVVGL
                     LSGSIACAPALPLMNRFGRH"
     gene            complement(8109..8567)
                     /locus_tag="WJK66_001218"
                     /pseudo
     CDS             complement(8109..8567)
                     /locus_tag="WJK66_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            8763..9044
                     /locus_tag="WJK66_001219"
     CDS             8763..9044
                     /locus_tag="WJK66_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578934.1"
                     /translation="MDWMGSLFLPGGALLFLSVVSTTLSARLGMPLLLVSPANVLDRA
                     AEALAIAAFLMLVARPPAVFGGLWKFNYSLREKAYISWLGLAEPCRFRR"
     gene            9044..9436
                     /locus_tag="WJK66_001220"
     CDS             9044..9436
                     /locus_tag="WJK66_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton exchanger"
                     /protein_id="HEZ7578935.1"
                     /translation="MMPPVTGVPNSHLLFDVAFAVVVLSLLIRGMTIPVMARLLKVAM
                     PNKPEPKDTHDIWLAKKEIVRMSAFKVVAESEAEGHHPDTVEPISDSFGARCFALIRN
                     GIRIEMQSDTVLQAGIWRGTSCPTARSI"
BASE COUNT         2082 a         2345 c         2788 g         2250 t
ORIGIN      
        1 gccgtactgg tttttgttaa tccactatat aaagctcgga gaaggtatat aaggacatca
       61 ttcctgcacc ggcaagaatc cgaacccgaa cgtttgaaaa caatcccgaa tctccgaatt
      121 cccgcctgtg cgggaatgac gaaaaaacaa gcatccattt gccccgaagg cagttaatca
      181 accctttccg ccacacacct attccaatat ccaatgaaaa ccatcacaga aaccctaaat
      241 ctcgccccga aaggcaaaaa cttcctgacc gccgattggc ccgcgcccgc caatgtgaaa
      301 accctgatta ccacgcgcaa cggcggcgtg agccaaggtg cgtatcagag tttgaacctc
      361 ggtacgcacg tcggcgacaa tcccgaagcc gtgcgccgca accgcgaaat cgtgcaacag
      421 caggtcggat tgcccgttgc ctacctcaat caaatccaca gtaccgtcgt cgtcaatgct
      481 gccgaagctt tgggaggcac acccgatgcg gacgcttccg tagacgacac gggcaaagtt
      541 gcctgtgctg tcatgactgc cgactgtctg cccgttctat tttgcgacag ggcgggtacg
      601 gcggttgccg ccgcacacgc gggctggcgc ggtttggcgg gcggcgtatt gcaaaacacc
      661 attgccgcga tgaaggttcc acccgtcgaa atgatggcgt atctcggtcc cgccatcagt
      721 gcggatgcgt ttgaagtcgg acaggatgtg tttgatgcgt tctgcacgcc catgcccgaa
      781 gccgccgccg catttgaagg cataggcggc ggcaaattcc ttgccgacct ttacgcgctc
      841 gcccgcctga ttctgaagcg cgaaggcgtg ggcggcgtat atggcggcac gcattgtacg
      901 gttttggaac gggatacttt cttttcctac cgccgcgacg gagcgacagg gcgtatggcg
      961 agcctgattt ggctggacgg caatgccgtc tgaacacgcc gctgatataa tctaccgact
     1021 ttgtgctttt gagaaaggca agccatgaac aaactgtttc ttactgccgc agtgctgatg
     1081 ctgggcgcgt gcggtttcca cctgaaaggt gcagacggca tttctccgcc gctgacctac
     1141 cggagctggc acatcgaagg cggacaggca ttgcagtttc ctttggaaac cgcgctgtat
     1201 caggcttcgg gcagggtgga cgatgctgcc ggcgcgcaga tgaccctgcg tatagacagc
     1261 gtttcccaaa acaaggaaac ctacaccgtt acccgtgcgg cagttatcaa cgaatatctt
     1321 ttaatcttga cggttgaagc gcaggtattg aaacgcggcg agccggtcgg caaaccgatg
     1381 accgtgtccg tccgccgcgt ccttgcttat gccgacaacg agatcttggg caaacaggaa
     1441 gaggaagcgg cattgtgggc ggaaatgcgg caggatgccg ccgaacagat tgtccgccgc
     1501 ctgacctttc tgaaggcgga atgacgtggc ggcacatatc ggacacattg atacggacgc
     1561 gcctttgaaa cccctgtacg tcatccacgg cgaggaagaa ctgttgcgta tcgaggcatt
     1621 ggacgcattg agggcggcgg cgaagaaaca aggttacctt aatcgggaag tttatacggc
     1681 agacaatgcc ttcgattgga acgaactgtt gcaaaccgca ggcagtgcgg gtctgtttgc
     1741 cgatttgaag ctgttggaac tgcatatccc caacggaaaa cccggcaaaa ccggcggcga
     1801 ggcgttgcag gattttgccg cccgcctgcc ggaagatacg gtaacgctgg ttttgctgcc
     1861 caaactggag aaaacccagc tccagtccaa atggtttgcc gcattggcgg caaaggggga
     1921 agtgtgggaa gccaaaccgg tcggcgcggc ggctttgccc caatggatac gcggacggct
     1981 ggacaaaatc ggtttgggta tcggggcaga cgcattggca ctgtttgccg agcgcgtgga
     2041 aggcaatctg ttggcggcgc gtcaggaaat cgacaagctc gggctgctgt atccgaaagg
     2101 gcataccgtc aatatcgatg aggcgcaaac cgccgttgcc aacgtcgccc gcttcgacgc
     2161 gttccaactg gcaggcgcgt ggatgaaggg cgatgtcctg cgcgtatgca ggcttttgga
     2221 cggattgcgg gaagagggcg aagaaccggt gctgttgctg tgggcggttg ccgaagacgt
     2281 gcggacgctg atccggcttg ctgccgccct gaagcagggg cagagtgtcc aatccgtacg
     2341 caacagtctc agactttggg gtgaaaagca gacgctcgca ccgcttgcag tcaagcggat
     2401 ttccgtcgtc cgcctgcttg acgcgctcaa aacctgcgcc caaatcgacc gaatcatcaa
     2461 aggtgcggaa gacggcgacg catggacggt attcaaacgg cttgtcgtgt cgctggcgga
     2521 ataaagcggt aatccccaaa atccgaaaat actgtaaaat accgttaatc ctgaaaagta
     2581 ttcaccaatc cgtccgaaaa catttcagac ggcacgacca cctcaataaa ggaacattaa
     2641 ccctatggac aataagacca aactgcgctt gggcggcctg attttactga ctaccgccgt
     2701 tttaagcctc attatcgtat tgattgtcga ctcctggccg cttgccatcc tgcttgccgc
     2761 cgtcatcgtc gccgccgctg cgggcggttt tgtttggaca tcccgccgac agcaacgcca
     2821 gtttatcgaa cgcctgaaaa aattcgacat cgatcccgaa aaaggcagaa tcaacgaggc
     2881 aaacctgcgc cgtatgtacc acagcggcgg acaacaccag aaagatgcga ttaccctgat
     2941 ctgcctgtcg caaaaatgtt cggtggacga ggcgcacgct atgttcaaaa aacgcccgac
     3001 acgtcaggaa atcaatcaaa tggcggcaaa acagtcgcgc ggtcagaaac gtccgcaccg
     3061 ttaaccgccg caaggcatct ttgcataaat gccgtctgaa gcctgttggc gtttcagacg
     3121 gcatattctg attgaaaaga tgatgacact gaaaaccgcc ccgctcaaac gccgctttgc
     3181 cgccatgctg tacgaaatgc tgctggtcgg tgcggcaacc tgtttggcag cattgtttgc
     3241 cggtattgcc gccatttttc tgaatcccgt ttctatcgcg gtttctgcat tggtaacgag
     3301 tatcctgata atgggatcat ggtggcttta ttttcgcgcc aactggcacg gtcaggggca
     3361 gaccttggcg atgaggacat ggaaaatcgg cttgtgcgac cttaacggca tacagccgtc
     3421 tttgcacctg ctgcgcctgc gctttatttg ggcgtgcata ttcatcgtct tcattcctat
     3481 gcttgcttat gccggattgc gccacttcct cggcattccg cccaagggcg cggtcggtgc
     3541 ggcattgatt tggctgattt taccgtgggg gttcgcactg ctgaatcccg atcggcagtt
     3601 tctgtatgat tttcttgcag gaacaagatt ggtggcggtc aaaggaaagc cttaagcctt
     3661 tataccgcaa aggtttcaac ctgaaaaaat gccgtctgaa agggctttca gacggcattt
     3721 gcttatcggg gaaaccgatt attcgatatt ctgcacctgt tcgcgcatct gttcgatcag
     3781 gactttcagc tcgaccgagg cttgggtgca ttcggcggca atggatttgc tgcccaaagt
     3841 gttggcttcg cggtttaatt cctgcatcag gaagtccagc cgttttccgc tgctgccttt
     3901 gtgttcggta acgatacggc gcacttcggc aatgtgggtg cgcaggcggc tgaattcttc
     3961 atcgacatcg gatttttgga caaacagggc aaactcttgt tgcaggcggt cgttgtcgat
     4021 gctgccgacg gcttcggcaa ggcgggcgcg gattttttct ttatgcgctt ccactagcgc
     4081 ggggaagatt tcgctcaatg ccccgatgat ttcttccata gactcgaggc gttgcagcag
     4141 gtgttcgccc aattttttcc cttcgcgtcg gcgtgcggcg gtaaagtctt tcagtgccga
     4201 atcggtcaaa tcggcaataa cctttgccaa tgcgtccgta ttttcccttt ggcttgccaa
     4261 tacgccgggg aaacgcagga tgtcggcaac gcccagtttt gccaaatcgt gatgcttgcg
     4321 gaggtctttg ttgatttcgg caagctgtcc gaccagatcg cgattcagtt ccaaagactg
     4381 attgccgttt tccgtatctt gaatttggat tttgcattcg actttgccgc gtgccagatg
     4441 ggatgaaatt ttctcgcgga taccgctttc caaatggcgc aaatcatccg gcatcctgat
     4501 ttgaatgtcc aaaaagcggt ggttgacggc gcggatgtcg aggttgatgc gtttgctgcc
     4561 gcattccgcc gccgcgttgg caaagccggt catgctgtgg atgtggatat ttccgctgct
     4621 catgtcgttc tccgaagccc gttaaaatgg aatcaatata tcacatctgt atggcggcaa
     4681 gcgttttcgg gtgtgaaaaa ttgaagattt tgcagcggca gattggaatc acgcgctttt
     4741 gttgctgcaa ggaagggaaa tgtatagtgg attaaccaaa accagtacgg cgttgcctcg
     4801 ccttagctca aagagaacga ttctctaagg tgttgaagca ccaagtgaat cggttccgta
     4861 cgatttgtac tgtctgcggc ttcgccgcct tgtcctgatt tttgttaatc cactatatca
     4921 attccgccaa tctgtcggaa aagcagctga tgcggcagtg tctggtgcat gtctgctttt
     4981 tgatttcggc gattgcgatg gtttggacgg ataaaatcgt gtacggcacg acgcacaaac
     5041 cgcattgatg tttaccaaat aaaatacccg acaaaacaat ttgtcgggta ttttattgcg
     5101 tatatttcaa accgcttcgg cttcttcggt caggaaacca cgcagtttct gcatggcttt
     5161 tgcttcgatt tggcggatgc gttcggcaga tacgccgtat tcggcggcaa gctggtgcag
     5221 cgtcagcccg ccgtcgtctt gaagccagcg gctttccaca atacggcggc tcctgtcatc
     5281 cagttgcgcc aaagcgtttt gtaaaccttc tgtttgcagg gcgtaatgcg cctgtttcga
     5341 tagttgccgg ctcggttcgg aatcgtggtc ggcaagccag tcgatggggg cgaaactgtc
     5401 ctcgtcgtcg ctgttgtctg ccatgatggc gatgtcgtgt cccgtcatgc gctgttccat
     5461 ttccagaact tcggaaagtt tgacacccaa atcgtcggcg atgtcttgtg cctctttggg
     5521 agacagggcg ttgaggtttt tacgcatact gcgtaaattg aaaaacagct tgcgttgcgg
     5581 tttggtggtg gcaacgcgaa ccaaacgcca gtttctcaaa ataaactcgt ggatttcggc
     5641 tttaatccag tgtacggcaa atgaaaacag acgcgcgcct ctaccgggct cgtagcgttt
     5701 gaccgccttc atcagtccga tattgccttc ctgaatcagg tctgcctgat tcagcccgta
     5761 gccgtcatag ccgcgcgcga tggaaacgac gacgcgcagg tgggacagga tgagttgttt
     5821 ggcggcgttg aggtcgccct ttatccggcg ttcggcgagg cgggtttctt cttcctggga
     5881 cagcatcggg atgctgttga cggtgtggat gtattgttcg aggctgccgt tgccgctttg
     5941 gatggcgggt aatgcgaaag cgttattcat ttggggcatt tcctttcggc tgaaactgcg
     6001 tatcggcggt ttgctgtgtt gggatgcagt atatcactgc ttggcttgta ttttgtatgt
     6061 ttggcaggag atatgcgcta aggtttgaaa gacaggaaaa ttttgtaagg caagtttgat
     6121 tgattttgta aacctgatgg ttcaattcta tttcagaatt atattacata cgtagttgta
     6181 tgtaaacggc cggtttgaaa aaagacagcc cgtccggacg ggctgtgcag gtatcagtgt
     6241 tctttgtttc ggaagatgaa aagaatcagt gcggctaggg tcaatatgcc catcaaccac
     6301 catgaactgc cggttttcat atagggcgtt tcgccgacat agcctttgat gtgtccttcc
     6361 aatacggttt ccgtatcggg ttgggcttgg gcgatgatgt tgcctttggg ggagatgatg
     6421 gcggttgcgc cggtgttggt ggcacggacc atatagcgtc cgagttccat agcccgcgcc
     6481 tgcgattgtt ggaggtgctg gtacatggcg ttggattttc cgtaccacgc catattgctg
     6541 gcattggcaa gcagggtggc atctttggcg gcggcaatca gttcgtcgcc gaatccgtct
     6601 tcgtaacaga tgttgaaggc gattttttgg tttttcatca gcagggcgga ttgcttaccg
     6661 ccgcctttgc ggaagtcgga aaggggcata ttcatcattt tgtaaagcgg cgtggtcagg
     6721 aagggcagcg gtttgtattc gccgaagggg acgaggtggt ttttggcgta gtaggggatg
     6781 ccgtcctgat tgttttcctg ataaccggtc aggttgatga cggcgttttc gtaaccgttg
     6841 ccgtccgaag tgtattggct gatgccgacg gcgagcgcgc tgccgttgtt ttgcgcctgt
     6901 tcggcaaatt ttgccagtat gttttccggc aggttttggc gcataacggg gatggcggtt
     6961 tcgggcagga tgacgatgtc ggcagtggtt ttgccgacct gttcgtaata tttctgtatg
     7021 gtcgggatga cttggtcttc acgccatttg agggtttggt cgatgttgcc ttgaagcagg
     7081 gcgacggtgc tgcggctgcc gtcggggcgg gtgaagtcgg tttgtcgggc ggtgtagcct
     7141 gcggcaagca gggcggcaat caggataatt ggaagcaggc gtttgcccga acgtgcggtg
     7201 ttatcactcg ccaaaaccag ccagacaccg agaaaggcgg ttgccagtgt aaccatgtgg
     7261 atgccgccca atggggcaaa gccggcgagc gggctgtccg gggtgatttg ggagtagccg
     7321 attgcgcccc agccgaatcc ggtcaggaaa cgttcgcggg caaactcggt cagcgtccac
     7381 aggatgggca gtaccaaacc gatttttatg ccccgaggca gggtaaattt tttccacagc
     7441 cagaaacaca gtgccggata aagggcaagg taggcgggga gtaggaaggt cagcggtacg
     7501 gcatagaggt cgggcaggcc ggaaacgtcg tgcagggcgg tgtgtatcca gtagaactgt
     7561 gtcgtgtatg cggtcaggcc gaacaggtag gcggaagaga cagcaaaacg cggacgcagt
     7621 tcgatgaggc ggacgaaggc accgaaaatc aagggcatca gccaaaagtg gtagtagggt
     7681 gcgaaggtaa agggggtggc ggcggcaaaa aggatgagca aaggccagta gagggcgggg
     7741 tgctgccagt atttgtccag tttggaaacc atattcatct gtctgttcgg aagataccgt
     7801 ctgaacatct ttcaaacggc atcggtattt gaaaaagaaa tcaatgcctg ccgaaacgat
     7861 tcatcagcgg caaggcgggg gcgcaggcaa tcgaaccgga aagcagcccg acaacgaggt
     7921 tggcgaagta ctccgcccag ccagcgtccc agtgttgcgc gtgcaggaaa tcgtgcagaa
     7981 ggccgaggtt gtgggcgacc agtacgccgc cgataagaaa catggcaagc gtgccgacca
     8041 cgctcaaacc gcgcataaag caaggcataa aggcagtcag catttgcccc aaactgcgcg
     8101 aaaaggtttg tgggcggcgc atcagcagca tgcctaagtc gtcgagtttg acgatgacgg
     8161 caacgattcc gtacaccaaa acagtcatgc cgatgccgat tgccgccatt acgagcatgc
     8221 gcgtcatgtc ggtgcagaaa cttgtgcagc agcttttcta cgccttcaaa gcacagataa
     8281 atgccgcctg ccgtcaaaag cggcgtaatg agttgcggca ggaaggcgga aagcagcagg
     8341 ggcgcaggca ccaaaaccgg cttgttggaa aaagaacctt tcgccatcga ccaaacaatc
     8401 ggcaactcgc gttctgccga tacgcccgta acccggttgg cattgggtgt caaatcgtcg
     8461 ccgaccatgc cggcggtttt ctttgcggcg gctttggtca tcagggcaac atcgtccaaa
     8521 acggcggtga tgtcgtccgg cagggtaaat agtgaggcaa atgccattaa agaatcctga
     8581 aatgcggcgc aaagtccgac attatatagg agaacgcgga tttgggcggt ttcaggcggc
     8641 atgaaacagg aaaatgccgt ctgaacgctg tggcggacgt gaagtaaagt ttcgtgaaaa
     8701 gaaaataccg tgttacagtc tttcgatttt aatttcatga attttaaggg agaatcgtta
     8761 gcgtggattg gatgggcagt ctgttcctgc cgggtggcgc actgttgttt ctgagcgtgg
     8821 tttcgaccac tttgtccgca cgtttgggaa tgcctttgct gctggtttct cctgccaacg
     8881 tgttggacag ggcggcggaa gccttggcga ttgcggcgtt cctgatgctg gtcgcgcgtc
     8941 cgccggcagt gttcggcggt ttgtggaaat tcaattacag cctgcgtgaa aaggcgtata
     9001 tcagttggtt gggtttggcg gagccgtgcc gatttcgccg gtgatgatgc cgccggtaac
     9061 gggcgttccc aattcgcacc tgctgtttga tgtggcgttt gccgtggtgg tgctgtcgct
     9121 gctgattcgg ggaatgacga ttcctgtgat ggcgcggctg ttgaaggttg ccatgcccaa
     9181 caagcccgaa ccgaaagata cgcatgatat ttggctggcg aaaaaggaaa ttgtcagaat
     9241 gtcggcgttt aaagtggttg ccgaatctga agcggaggga caccatcccg atacggtcga
     9301 accgatttcc gattcgtttg gcgcacgctg tttcgcattg atacgcaacg gcatccgaat
     9361 agaaatgcag tccgacaccg tgcttcaggc ggggatttgg cgtggtacat cctgcccgac
     9421 ggcaaggtcg atatagtgga ttaacaaaaa tcaggacaag gcggc
//
LOCUS       DAWKOG010000069         9432 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.069, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000069 DAWKOG010000000
VERSION     DAWKOG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.069"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(138..1580)
                     /gene="aldA"
                     /locus_tag="WJK66_001221"
     CDS             complement(138..1580)
                     /gene="aldA"
                     /locus_tag="WJK66_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEZ7578936.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEETIAREPKGGKADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRGRADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVSA
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
     gene            complement(1615..1815)
                     /locus_tag="WJK66_001222"
     CDS             complement(1615..1815)
                     /locus_tag="WJK66_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578937.1"
                     /translation="MDTVGSLAIGYATGGNAGAFTDANADWNNRQLHSSEYKLAEQYA
                     DIVASRLNISKETCTAIYLGIN"
     gene            complement(1824..2213)
                     /locus_tag="WJK66_001223"
     CDS             complement(1824..2213)
                     /locus_tag="WJK66_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578938.1"
                     /translation="MLYKCKIKKLDNTIEEAVLIEINGYELHCFISYCPFKIFENQFY
                     EVELSYEIFNDYILDKSTSNIGFRKIDDSFKYEITAELKNNHLTLQNIQFEEDEYLVN
                     YSYLDNCLVTLTADRISVSFIREIQAA"
     gene            complement(2422..3339)
                     /locus_tag="WJK66_001224"
     CDS             complement(2422..3339)
                     /locus_tag="WJK66_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7578939.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYVEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLDFGINRCRFQIDADGGKIVLIAVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYARFAPLLYREPVRIWDLPPNLRGLAASCLKAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFSRTLNAAFVGGA
                     HQVNRLTDALNISERTLQRRMRDHFGITASEWLHHKQMQHALYLLQNGGKSVGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            3511..4311
                     /locus_tag="WJK66_001225"
     CDS             3511..4311
                     /locus_tag="WJK66_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ7578940.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILNDINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLSGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTQLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            4359..5135
                     /gene="mlaE"
                     /locus_tag="WJK66_001226"
     CDS             4359..5135
                     /gene="mlaE"
                     /locus_tag="WJK66_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEZ7578941.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVTWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAIDFVLTAWMFTD"
     gene            5187..5681
                     /gene="mlaD"
                     /locus_tag="WJK66_001227"
     CDS             5187..5681
                     /gene="mlaD"
                     /locus_tag="WJK66_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEZ7578942.1"
                     /translation="MKKNILEFWVGLFVLLGAAAVAFLAFRVAGGAAFGGSDKTYAVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            5718..6308
                     /locus_tag="WJK66_001228"
     CDS             5718..6308
                     /locus_tag="WJK66_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HEZ7578943.1"
                     /translation="MKKSSFISALGIGILSIGMAFAAPADAVNQIRQNATQVLSILKS
                     GDANTTRQKAEAYAIPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGSK"
     gene            6365..6643
                     /locus_tag="WJK66_001229"
     CDS             6365..6643
                     /locus_tag="WJK66_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HEZ7578944.1"
                     /translation="MHTELNNGTLHISGDVTVKTLTGDAFERFRQQCRLKETIAVDFG
                     GVKRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            6640..7467
                     /locus_tag="WJK66_001230"
     CDS             6640..7467
                     /locus_tag="WJK66_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEZ7578945.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            7592..7954
                     /locus_tag="WJK66_001231"
     CDS             7592..7954
                     /locus_tag="WJK66_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HEZ7578946.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            7951..8337
                     /locus_tag="WJK66_001232"
     CDS             7951..8337
                     /locus_tag="WJK66_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ7578947.1"
                     /translation="MMKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELA
                     GLILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITL
                     MPVHAATQRTVSLPATLARALEALSE"
     gene            8334..8822
                     /locus_tag="WJK66_001233"
     CDS             8334..8822
                     /locus_tag="WJK66_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ7578948.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANDYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(8904..9071)
                     /locus_tag="WJK66_001234"
     CDS             complement(8904..9071)
                     /locus_tag="WJK66_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEZ7578949.1"
                     /translation="MEDDVWPGRRVIVMAGVTVGRGSVVGAGAVVTKDIPPYSLAAGN
                     PAVVKKNLPEG"
     gene            complement(9038..9412)
                     /locus_tag="WJK66_001235"
     CDS             complement(9038..9412)
                     /locus_tag="WJK66_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HEZ7578950.1"
                     /translation="MGSKFFFLLLRFAGSVLPPSHMRGIVGRRVRGFLARRVSPHIGR
                     GVNIERGAYVFPDTVLGDGSGIGANCEICRGPVVGKNVMMEPECLFYSNNHKFDRSKN
                     ALRATRKSVRLRWRTMSGRGAG"
BASE COUNT         2182 a         2644 c         2365 g         2241 t
ORIGIN      
        1 cgtttaaatt taatccgcta tacaaagaca aaaacatcga cctcggtcat gactgattgc
       61 cgatgaagca ataaaaatgc cgtctgaacc tgaaaacggg ttcagacggc attttctatc
      121 ggggttttaa agcggcatta aatgtcggtt tccaaataaa cgacttgggt ttgcagatat
      181 tcttccaaac cgtgtttgcc gtccgcgccg ccgataccgg attttttcca accggcgtgg
      241 aaaccctgca tcgcttcaaa gttttcgcgg ttgatgtagg tttcgccgaa ttgcaggcgg
      301 cgggtaacgt agaaggcttc gtttaaatta gtcgtataaa cagaactggt cagaccaaac
      361 tcgcaatcgt tagccagtgc gatgacctgg tcgagcgtgt cgaaagcgga aacgggcagc
      421 acggggccga aggtttcttc tttcataatg tccatactgt tgtcggtgtc ggtcagcagg
      481 gtcggctcga agaaataacc gcgtccttcg gcgcgtttgc cgccgcaaac caatttcgca
      541 ccttgtttga ctgcccgttc cactttttcg gcaacggctt tgacggcgcg ctcttcaatc
      601 agcgggccca tttccagcgc gcctgcttcg gcttcggcag ggttgccgta gcgtacgcct
      661 ttcatggcgg cggtcatttt ttcaatgaat gcgtctttca gactgctgtg gacataaacg
      721 cgctcggcgc agttgcagat ttgaccagtg ttgccgacgc gcgaagccaa gatggatttc
      781 actgccaagt ccaaatccgc gtctttcaaa acgatagcag gtgctttgcc gccgagttcc
      841 agcgagactt tggtgatgtt ggcagaagcg gcttccatca cttggcggcc cgcttcaacg
      901 gagccggtca ggctgaccat atcgacttgc ggatgggcgg acaaggcatt gccgatttcc
      961 gcacccggac cgttcacaac gttgaacacg cctgctggca gtccgaccgc atcgacgatt
     1021 tcggcgaaga tgtggcagtt gatcggggtt acgctgctgg gtttgacgac gatggtgttg
     1081 cccgttacca aagcggggcc cattttgcgg gcaatcagga agaaggggaa gttccacggc
     1141 aaaatgccgg cgattacgcc cagcggacgt ttgaacaata aaatattttc gcgcgggcgg
     1201 tcgctttgga tgatttcgcc ttcgtaacga cgcgcccatt cggcttggta atcgagatag
     1261 tcggcggtaa acatgacttc cacgcgcgcc aagtctttgg ttttgccgcc ttcggcaacg
     1321 atggtgtcgg tcagctcgtc ggcgcgtccg cgtatgcctt gggcgatttt gcgcaaatac
     1381 gcgccgcgtt cgaccgcagg caggcgttcc caagccggtt gcgccgcgcg tgccgccgcc
     1441 acagctcggt caacgtctgc ttttccgcct ttgggttcgc gggcgatggt ttcttcggtg
     1501 gatgggttca atacgtcgcg ccattcgccg ttgaaatcgt tttcaaagcg tccgttgatg
     1561 tacatggcca attgtttcat ttcgggttct ccagttttgt agtcagatgt agtcttagtt
     1621 tattcccaaa taaattgccg tacaagtttc ttttgaaatg tttagacgag aagctactat
     1681 gtctgcatat tgttctgcta atttatattc actcgaatgc aactgccgat tattccaatc
     1741 tgcattcgca tcagtaaaag ccccagcatt gccgcctgtg gcataaccaa tcgctaaact
     1801 gccaacggta tccacaacag gtttcaggct gcctgaattt ctctaataaa tgaaacactt
     1861 attctatcag ccgttaatgt aaccaaacaa ttatcaagat aagagtagtt tactaaatac
     1921 tcatcttctt caaattgtat attttgtagg gttagatgat tattttttaa ttctgccgtt
     1981 atttcatact tgaaactatc atctattttt ctaaatccaa tatttgaagt tgatttatcc
     2041 agtatgtaat cattaaagat ttcataggat aattccactt cataaaattg attttcaaat
     2101 attttgaatg ggcagtatga tataaaacaa tgtagttcat aaccatttat ttcaatcaat
     2161 actgcctctt caatggtgtt atctaacttt ttgattttac atttatataa cattttatct
     2221 tgcctttaga taaattgttt tgttttttgg attgttattc aaagttctcc aaaagtcttt
     2281 tcctgatttt gtatgtatcg tggtaacact accattttca ttggttccaa caaaaataaa
     2341 tttagattga cctttggaat cagtacccaa aaactgaaca tatccagaca tgcggatttc
     2401 gggttttaaa tcaaatgcgg cttaccggtt ttcttttttg gtttccgccg gcgtgctgcc
     2461 gaaatattgc ctgaatgcct gagtaaagct ggaaacgtgg cggtagccgc ataaatacgc
     2521 ggtttcgcct acgcttttgc ccccgttttg caacagatag agcgcgtgct gcatttgttt
     2581 gtggtgcagc cactcgcttg ccgtaatgcc gaaatggtcg cgcatacggc gttgcagcgt
     2641 cctttcgctg atgttcagcg cgtctgtcag ccggttgact tggtgtgcgc cgccgacaaa
     2701 cgcggcattc agggtgcggc tgaagtcttc agacggcata gcgtctgctt ccgccgtttg
     2761 ccccgccgcc ggcccgatgc cgtctgaaac cgtgtcccac aagtccgaca gcagccgcaa
     2821 aacgtccgcc tcgcggcgca atgtttcgcc caaatgcccc tttgggacgg ctttcaggca
     2881 ggatgccgcc aagccgcgca ggttcggggg caaatcccat atcctgaccg gttcgcggta
     2941 aagcaggggt gcgaaacgcg cgtattccgg acgcagcagc cattgttcca tacctttaat
     3001 ggtcattttg accgttttgc cgcctcggta aagatagcgg ctgaacagga cttcttcccc
     3061 gacagcaatt aggacaatct tgccgccgtc cgcatcgatt tggaagcggc agcggttgat
     3121 gccgaagtcc aaactgcctt ccagcaagag cacgaacgac acataaggtt cggcgaggcg
     3181 gctgctgcaa aaatcacacc gtgccgttac cgtgccgccg tggatggaaa tgccgttgga
     3241 caaagtatcg aaacggtagc tgccctccac atagctgccg ccgccgcttt tgaccagatt
     3301 gacgaaccgg ctgctgttca aatgttctgc tttttccata gttttctgat aaaggttatc
     3361 atttgaaaga taacattttt cgccacagca aacaatccgc cgccacaacg gcattttcac
     3421 aacacttgcc ggccgtatgc cgtctgaaaa ttgcccgtct tcccgataag ccttaaaata
     3481 cacgccgtca aacctaccgt ccgtccgtac atgagcccat ccccctttat cgaaatgaaa
     3541 gacgtcgctt ttgcgtatgg cgaccgcccg attctgaacg acatcaattt cagcattccg
     3601 caaggcaatt ttgccgccgt gatgggcggt tcgggcagcg gcaaaaccac gctgatgcgg
     3661 ctgattacag ggcagattcg tccgcagtcc gggcaggttt tgattgaagg gcgggatttg
     3721 tcgggctttt cggctgacga actctacgaa caccgccgcc gtatgggcgt attgttccaa
     3781 cacggcgcgc tgtttactga tttgtctgta tttgacaata tcgcttttcc gatgcgcgaa
     3841 ctgacgcagc tgccggaagc ggtgattcga gatttggttt tgttgaaact gaacgcggtc
     3901 ggtctgcgcg gtgtagaaaa cctgatgccg tccgagttgt ccggcgggat gtcgcgccgc
     3961 gtcgcgcttg cccgcacgat tgcgctcgac cccgaaatta tgttgtacga cgaaccgttt
     4021 accggtctcg atccgatttc cttgggcgtg attgcccact taatcagccg cgtcaacaag
     4081 gctttgcgtt cgaccagtat tatggtaacg cacgacattg aaaaatcttt ggaaatcgtc
     4141 gatcaggtga ttttcttggc gcacggcgaa attatgttct ccggctcgcc gcaggaaatg
     4201 cgcgaactgg attcgccttg ggtgcgccag tttgtcggcg gcttggcaga cggtcccgta
     4261 gcataccgct atccggcgca aacgtcgttg cagcaggacc tgttgggata aatgagttga
     4321 tgccgtctga aaccgttgaa ctttacacat agaaatttat gaactttatc cgttccgtcg
     4381 gggcgaaaac cctcggcctt attcaatccc tcggcagtat cacgctgttt ctgctgaaca
     4441 ttctggcgaa gtccggtacg gctttcgtcc gtccgcgcct gagcgtgcgc caagtgtatt
     4501 tcgccggcgt gctgtcggtg ttgattgttg ccgtttcggg gctgtttgtc ggtatggtct
     4561 tgggtttgca gggctatacg cagttgtcga aattcaaatc cgccgatatt ttgggctata
     4621 tggtcgcggc ttcgctgttg cgggaactgg gtccggtgtt ggcggcgatc ctgtttgcca
     4681 gcagcgcggg cggtgcgatg accagcgaaa tcggtctgat gaaaacgacc gaacagctcg
     4741 aagcgatgaa cgtgatggcg gtaaaccctg tcgcccgcgt ggttgcgccg cgcttttggg
     4801 cgggcgtgtt ttccatgccg cttttggctt cgattttcaa cgtggcgggt attttcggcg
     4861 cgtatttggt cggtgtaacc tggctgggct tggacagcgg cattttctgg tcgcaaatgc
     4921 agaacaacat cacgatacac tacgatgtaa tcaacggtct gatcaagtcc gccgcgttcg
     4981 gcgtggcggt aacgctgatt gccgtgcatc agggcttcca ttgcgtcccg acctcggaag
     5041 gcattttgcg cgccagcacg cgtacggtgg tttcgtccgc cctgacgatt ttggcgatcg
     5101 actttgtgtt gaccgcgtgg atgtttacag attgacaggc cgtctgaaaa cgaaacaaaa
     5161 aacatttgga tattcaagga actttaatga aaaagaacat attggaattt tgggtcggac
     5221 tgtttgtcct gctcggcgcg gcggcggttg cctttctcgc tttccgcgtg gccggcggtg
     5281 cggcgttcgg cggttcggac aaaacttacg ccgtttatgc cgatttcggc gacatcggcg
     5341 gtttgaaggt caatgccccc gtcaaatctg caggcgtatt ggtcgggcgc gtcggcgcta
     5401 tcggacttga cccgaaatcc tatcaggcga gggtgcgcct cgatttggac ggcaagtatc
     5461 agttcagcag cgacgtttcc gcgcaaatcc tgacttcggg acttttgggc gagcagtaca
     5521 tcgggctgca gcagggcggc gacacggaaa accttgctgc cggcgacacc atctccgtaa
     5581 ccagttctgc aatggttctg gaaaacctta tcggcaaatt catgacgagt tttgccgaga
     5641 aaaatgccga cggcggcaat gcggaaaaag ccgccgaata aacgtatgcc atcatattca
     5701 aactaggaca cttaatcatg aaaaaatcct ccttcatcag cgcattgggc atcggtattt
     5761 tgagcatcgg catggcattt gccgcccctg ccgacgcggt aaaccaaatc cgtcaaaacg
     5821 ccactcaagt attgagcatc ttaaaaagcg gtgatgccaa caccacccgc caaaaagccg
     5881 aagcctatgc gattccctat ttcgatttcc aacgtatgac cgcattggcg gtcggcaacc
     5941 cttggcgcac cgcgtccgac gcgcaaaaac aagcgttggc caaagaattt caaaccctgc
     6001 tgatccgcac ctattccggc acgatgctga aattaaaaaa cgccaacgtc aacgtcaaag
     6061 acaatcccat cgtcaataaa ggcggcaaag aaatcatcgt ccgcgccgaa gtcggcgtac
     6121 ccgggcaaaa acccgtcaac atggacttca ccacctacca aagcggcggt aaataccgta
     6181 cctacaacgt cgccatcgaa ggcgcgagcc tggttaccgt gtaccgcaac caattcggcg
     6241 aaatcatcaa agcgaaaggc gtggacggac tgattgccga gttgaaggct aaaaacggca
     6301 gcaagtaatc cataagccat gccgccagtc tgaaggcggc atggattttt tcctaccgaa
     6361 cactatgcat acagaactca acaacggaac actgcacatc agcggcgacg taaccgtcaa
     6421 aaccctgacc ggggacgcat ttgagcgttt ccggcagcaa tgccgtctga aagaaaccat
     6481 tgccgtcgat ttcggcggtg tcaaacgtgc cgattccgcc tgcgtgtcgc tgctgctcga
     6541 agtgctgcgc ggctgcaaag gcagcgtcag gctgaccggc attcccgaat ccgtgcgcgc
     6601 gctgtccgaa ctgtacgaaa tcaaagactg gctgaaatca tgaaaaaaac cgcctatgcc
     6661 ttcctcctgc tgatcgggtt cgcttccgcc cctgcatttg ccgaaacccg ccccgccgac
     6721 ccttatgaag gctacaaccg cgccgttttc aaattcaacg accaagccga ccgctacatt
     6781 ttcgcccctg ccgcgcgcgg ctaccgcaaa gttgcgccga aacccgtccg cgccggcgtg
     6841 tccaattttt ttaataacct gtgcgacgtg gtcagcttcg gcagcaatat cttgcgcttg
     6901 gacatcaaac gcgcaagcga agaccttgtc cgcgtcggca tcaacaccac tttcggtttg
     6961 ggcgggctta tcgacatcgc cggcgcgggc ggcattcccg acaataaaaa caccttgggc
     7021 gacacgtttg cttcgtgggg atggaaaaac agcaattatt tcgtgttgcc cgtcttaggg
     7081 ccgtccaccg tccgcgacgc gctcggcacg ggtattacct ccgtttattc gcccaagaat
     7141 atcgtcttcc gcacccctgt cggacgctgg ggcacgactg ccgtatccgc cgtcagtacg
     7201 cgcgaaggcc tgctcgattt gaccgacagc ctggacgaag ccgccatcga caaatacagc
     7261 tacacgcgcg acctctatat gaaagtccgt gcgcggcaga ccggtgcaac acctgccgaa
     7321 ggtacggaag ataacatcga catcgacgaa ttggtcgaaa gtgccgaaac cggcgcggcg
     7381 gaaactgccg ttcaagaaga ttccgtatcc gaaacacagg cagaagcagc aggggaagcc
     7441 gaaacgcaac ctggaacaca accttaaagc catgccgtct gaaaatccgt gttcggacgg
     7501 cattcccttc tgtcggaacg gacagtccga aaccacagat tgggctataa tctctttttt
     7561 gcgcataccg cgcgacagca aaggaaaaac catgtacgaa gtcaaccgca gcgtattcgt
     7621 cctcatccct ttagaacctt tttggaactg gctgcaaacc ctgccgggca accacctcga
     7681 cggactgact cttgaagaca tccaagccga cgccaattcc taccttgtcc gcccgtgcga
     7741 aaccgccgac gaagtatggg acgaaatcga agcccgtttt gaagacattt tcgccgccga
     7801 acttgccgac tggtgcgaag acgaacgcga gtggcctgcg ctcgatgccg acattttcaa
     7861 cgaatggttc gacatccagc tttctaccgt catcaccgac ctcgaacacg aaccgcttgc
     7921 ccgcgaagcc ttccaaccca tcaacctgaa ctgatgaagc tgaccgtccg caactaccat
     7981 ctcgacggct acggccatgt caacaatgcg cgctacctcg aatttttcga agaagcgcgc
     8041 tgggcgtttt ttgaagaacg cggactgctg cacgagttgg caggcctcat actgattgtc
     8101 gcccgcatcg acatccgata cagccgcccc gccgtcgaag gcgacgtatt gcagttttca
     8161 tgccgcctga aaacccccgg cacgcgccgt attgtgctga cccagacgat tacactgcca
     8221 aacggcaaaa ccgccgcaga agccgacatc accctgatgc ccgtccacgc cgccacacaa
     8281 cgcaccgtca gcctgcccgc cacccttgcc cgcgcattgg aagccttgtc cgaatgaaaa
     8341 aaatactcac cgccgccgtc gtcgcactga tcggcatcct ccttgccatc gtcctcatcc
     8401 ccgacagcaa aaccgcgccc gccttctccc tgcccgacct gcacggaaaa accgtttcca
     8461 acgccgacct gcaaggcaaa gtaaccctga ttaatttttg gtttccctcc tgtccgggtt
     8521 gtgtgagcga aatgcccaaa atcattaaaa cggcaaatga ctataaaaac aaaaacttcc
     8581 aagtccttgc cgtcgcccag cccatcgatc cgatagaaag cgtccgccaa tatgtcaaag
     8641 actacggttt gccgtttacc gtcatgtatg atgcggacaa agctgtcgga caggcgttcg
     8701 gcacacaggt ttatccgact tccgtcctta tcggcaaaaa aggcgaaatc ctcaaaacct
     8761 acgtcggcga acccgatttc ggcaaactct accaagaaat cgataccgcg ctggcacaat
     8821 agcgatgccg tctgaaacgc aaaaaaacag aaacgatgtt cctgtttttt cagatcatcc
     8881 gccccgacac gttcagacgg cattcaacct tccggcagat tctttttcac cactgccgga
     8941 ttgcctgccg ccaaagagta gggcggaatg tcttttgtaa ccaccgcgcc tgcgcccacg
     9001 accgaaccgc gtccgacggt tacgcccgcc attacaatca ccctgcgccc cggccagaca
     9061 tcgtcctcca acgtaatcgg acggatttcc gtgtagccct caaagcgttt tttgaacggt
     9121 caaacttgtg gttatttgaa taaaacagac attccggctc catcatcaca tttttgccga
     9181 ccaccggccc acggcagatt tcacagtttg ccccgatgcc cgagccgtcg cccaaaaccg
     9241 tatccggaaa cacatacgcc ccgcgttcga tattgacccc gcgtccaata tgcggagaaa
     9301 cccgccgcgc caaaaaaccg cgtacccgtc tgccgacgat gccgcgcata tgcgacggcg
     9361 gcaacaccga accggcaaaa cgcagcagca ggaaaaagaa ttttgatccc atcaaacctc
     9421 cctcaaaaga ac
//
LOCUS       DAWKOG010000070         9334 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.070, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000070 DAWKOG010000000
VERSION     DAWKOG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9334
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.070"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            164..1507
                     /locus_tag="WJK66_001236"
     CDS             164..1507
                     /locus_tag="WJK66_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HEZ7578951.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPVITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPVSGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYAPEALANLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPVVVIKEAAEDFRRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFATGRLNTERVIALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAITQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            1510..2742
                     /locus_tag="WJK66_001237"
     CDS             1510..2742
                     /locus_tag="WJK66_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HEZ7578952.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYALAN
                     ALGINMSSEAGVLGKMLFGAIYFLPIYATVFIVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            2735..3511
                     /locus_tag="WJK66_001238"
     CDS             2735..3511
                     /locus_tag="WJK66_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HEZ7578953.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVKDAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            3511..4137
                     /locus_tag="WJK66_001239"
     CDS             3511..4137
                     /locus_tag="WJK66_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HEZ7578954.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGMMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            4141..4734
                     /gene="nqrE"
                     /locus_tag="WJK66_001240"
     CDS             4141..4734
                     /gene="nqrE"
                     /locus_tag="WJK66_001240"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HEZ7578955.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPANQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYGFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            4748..5965
                     /gene="nqrF"
                     /locus_tag="WJK66_001241"
     CDS             4748..5965
                     /gene="nqrF"
                     /locus_tag="WJK66_001241"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HEZ7578956.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPEEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            6118..7173
                     /locus_tag="WJK66_001242"
     CDS             6118..7173
                     /locus_tag="WJK66_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ7578957.1"
                     /translation="MPSETRLPNFIRTLIFALSFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPNSEISRFNQTTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     KGGYRTAMSSEFEKLLR"
     gene            7199..7414
                     /gene="nqrM"
                     /locus_tag="WJK66_001243"
     CDS             7199..7414
                     /gene="nqrM"
                     /locus_tag="WJK66_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HEZ7578958.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(7538..8116)
                     /gene="grpE"
                     /locus_tag="WJK66_001244"
     CDS             complement(7538..8116)
                     /gene="grpE"
                     /locus_tag="WJK66_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEZ7578959.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            8300..9118
                     /gene="cysE"
                     /locus_tag="WJK66_001245"
     CDS             8300..9118
                     /gene="cysE"
                     /locus_tag="WJK66_001245"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEZ7578960.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
BASE COUNT         2258 a         2596 c         2327 g         2153 t
ORIGIN      
        1 aacagcttcc gcaaggaaaa aagcagcaaa tctgacaaac aggtacaatt cctgatagaa
       61 tctaacactt tgctacatta atttattcaa tgcttttgat gccacgcgac acggtatcaa
      121 aacacgccgt ttttacctta accatcacaa gtaatgcaaa tatatgatta aaatcaaaaa
      181 aggtctaaac ctgcccatcg cgggcagacc ggagcaagtc atttatgacg ggcccgtcat
      241 taccgaagtc gcgttgcttg gcgaagaata tgccggtatg cgcccctcga tgaaagtcaa
      301 ggaaggcgat gccgtcaaaa aaggccaagt gctgtttgaa gacaaaaaga atccgggcgt
      361 ggtgtttacc gcgccggttt caggcaaaat cgccgccatc catcgcggcg aaaagcgcgt
      421 acttcagtcg gtcgtgattg ccgttgaagg caacgacgaa atcgagttcg aacgctacgc
      481 gcccgaagcg ttggcaaact taagcggcga agaagtgcgc cgcaatctga tccaatccgg
      541 tttgtggact gcgctgcgta cccgtccgtt cagcaaaatc cctgccgtcg atgccgagcc
      601 gttcgccatc ttcgtcaatg cgatggacac caatccgctg gcggcagacc ctgtggttgt
      661 gatcaaagaa gccgccgagg atttcagacg aggtttgctg gtattgagcc gtttgaccga
      721 gcgtaaaatc catgtgtgta aggcagctgg cgcagacgtg ccgtctgaaa atgctgccaa
      781 catcgaaaca catgaattcg gcggcccgca tccggccggt ttgagtggca cgcacattca
      841 tttcattgag ccggtcggtg caaacaaaac cgtttggacc atcaattatc aagatgtaat
      901 tgccatcgga cgtttgtttg caacaggccg tctgaacacc gagcgcgtga ttgctttggg
      961 tggttctcaa gtcaacaaac cacgcctctt gcgtaccgtt ttgggtgcga aagtatcgca
     1021 aattactgcg ggcgaattgg ttgacgcaga caaccgcgtg atttccggtt cggtattgaa
     1081 cggcgcgatt acacaaggcg cgcacgatta tttgggacgc taccacaatc agatttccgt
     1141 tatcgaagaa ggccgcagca aagagctgtt cggctgggtt gcgccgcagc cggacaaata
     1201 ctccatcacg cgtacgaccc tcggccattt cctgaaaaac aaactcttca agttcacgac
     1261 agccgtcaac ggtggcgacc gcgccatggt gccgattggt acttacgagc gcgtaatgcc
     1321 gctagacatc ctgcctaccc tgcttttgcg cgatttaatc gtcggcgata ccgacagcgc
     1381 gcaagcattg ggttgcttgg aattggacga agaagacctc gctttgtgca gcttcgtctg
     1441 cccgggcaaa tacgaatacg gcccgctgtt gcgtaaggtg ctggaaacca ttgagaagga
     1501 aggctgatta tgggcttgaa acattttctg gaaaaaatcg aaccgcactt cctgccgggc
     1561 ggcaaacatg aaaaatggta tgccctctac gaagctgcgg cgacgatttt ctatacatcc
     1621 ggtgcggtaa cgcgcaaagc ggcacacgtc cgcgatgcgc tcgactccaa acgcatgatg
     1681 attttggtgt ggctggcttt gttccctgcc atgttctacg gtatgtacaa cgtcggcgca
     1741 caggcattcg gtgcgttaac gcccgatttg ctgcaacaaa gcatcgccaa cgactggcat
     1801 tacgcccttg ccaacgcttt gggcatcaat atgtcgtctg aagcgggcgt gttgggcaaa
     1861 atgctgttcg gcgcgattta cttcctgccg atttacgcga ccgtatttat tgtcggcggt
     1921 ttctgggaag ttttgttcgc caccgtgcgc aaacatgaaa tcaacgaagg tttctttgtt
     1981 acctcgattc tgtttgcctt aatcgttccg cccacgctgc cgctgtggca ggcagctttg
     2041 ggtatttctt tcggcgttgt ggttgcgaaa gaagtattcg gcggtacagg taaaaacttc
     2101 atgaaccctg cgctggcagg ccgtgccttc ctgttcttcg cctaccctgc caacttgagc
     2161 ggcgatgcgg tttggacggc ggttgacggc tattccggcg caaccgcgct ggcgcaatgg
     2221 gcggcacacg gtgcagacgg cctgaaaaac gccgtaaccg gtcaaaccat cacttggatg
     2281 gatgcgttta tcggcaaact gcccggctcc atcggcgaag tctccacttt ggcactctta
     2341 atcggcggcg cgtttatcgt gtttgcccgc atcgcttctt ggcgcattat tgccggcgtg
     2401 atgatcggta tgattgccat gtcttcgctg ttcaacttca tcggttcgga caccaacgct
     2461 atgtttgcta tgccttggta ctggcatttg gtcgtcggcg gcttcgccat cggtatgctg
     2521 tttatggcga ccgaccccgt ttccgcttcc tttaccaatg tcggcaaatg gtggtacggc
     2581 gcactgatcg gtgtgatgtg cgtattaatc cgcgtggtca atccggctta ccccgaaggc
     2641 atgatgttgg cgattctgtt tgccaacctg tttgccccga ttttcgacta tttcgtcgca
     2701 caagcgaaca tcaaacgcag aaaggcgcgc agcaatggct aagaaattcg ataaagacag
     2761 cttcagcggc acgctgattg tcgtgttggc ggtcagcctg atttgctcgg tcatcgttgc
     2821 cggtgcggtc gtcggcttga aacccatcca agagaaacaa aaactccaag acaaacaagg
     2881 ctatatcttg agcgtagccg gtttgatgga taaggacacc gacatcggta aaacttttgc
     2941 cgagcgtatc gagcaacgcg tggtcgattt ggcgaccggc gaatatgtga aagacgcgcc
     3001 gaaagacttc agtgcgcgca tcgcaggcaa agaccccgcg caaagcatcc gcatcaaacc
     3061 cgaagacgat ttggcaggca tcaaaagccg tgccaaatac accgaggttt acttggtaaa
     3121 aggtgaagac ggcaaaatcg gccaaatcat cctgcctatg cacggtaacg gtttgtggtc
     3181 ggtcatgtac ggctttgtcg ccatccaacc cgacggcaac accatcaacg gcattaccta
     3241 ctacgagcaa ggcgaaaccc cgggcttagg cggcgaaatc ggcaatcctt tgtggcagca
     3301 aaaattcgtc ggcaaaaaac tgtttgacgg acaaggcaaa ctcgccctgc acgtcggcaa
     3361 aggcgcgggt tcggacaaag aacacggcgt agatgccctc tccggcgcgt cgctgacatc
     3421 caaaggcgta caaggttcgt tcgcctactg gttcggcgaa aacggctata tcccctacct
     3481 gaacaaattg aaatcagcag gagcgcaata atggctgata tgaaacgctt gaaacatttg
     3541 atgttttcac cctttatcga caacaacccg attgccttgc aggttttggg tatttgttcg
     3601 gctttggcgg ttaccaccaa acttcagacg gccatcgtga tgggtatttc cgtagctttg
     3661 gtaaccggtt tttccagctt cttcatttcg ctggtacgca actacatccc caacagcatc
     3721 cgcatcatcg tgcaaatggc gattatcgcg tcgctggtta cgctggtcga ccaactcttg
     3781 caggcatttg cctacgaatt gtccaaacag ctttccgtat tcgtcggtct gattattacc
     3841 aactgtatcg tgatgggccg cgccgaagca tttgcgatga aagagccgcc gctggaaagc
     3901 ctgatcgacg gcatcggcaa cggcgcgggc tacgggatga tgctgcttgt cgtcgccacc
     3961 gtccgcgaac tgattggctc gggcaaactc ttgggctaca ccgttttcca aaccgtgcag
     4021 gacggcggct ggtatcagac caacggcttg ttcctgctcg cccccagcgc gttcttcatc
     4081 atcggctttt tgatttgggg actgcgtacc tggaaacccg aacaggcgga ggaataagct
     4141 atggaacact atttgagcct ctttatcaaa tccgtcttca tcgaaaatat ggcgctatcc
     4201 ttctttttgg gtatgtgtac gtttttggcg gtatccaaaa aagtatccac cgcattcggt
     4261 ttgggtgtgg cggtgatttt cgtactcgga ctgtccgtcc ccgccaacca attggtttac
     4321 tcgctgctca aagacggcgc gattgtcgaa ggcgtggatt tgaccttttt gaaattcatc
     4381 accttcatcg gcgtgattgc cgctttggtg cagattttgg aaatgttctt ggataaattc
     4441 gtccccgccc tctataacgc gctgggcatc tacctgccgc tgattaccgt aaactgcgcg
     4501 attttcggtg ccgtatcgtt tatggcgcag cgcgaatacg gcttcggcga atccgtcgtg
     4561 tacggcttcg gtgcgggctt gggctggatg ttggcgattg tcgctttggc gggcattacc
     4621 gaaaaaatga aatattcgga cgctcccaaa ggcctcaaag gcttgggcat tacctttatc
     4681 gccgccggcc tgatggcgat ggcgtttatg tcgttctctg gcatccagtt ataagaaagg
     4741 gatcggtatg gagattattt taggtatcgt gatgtttacc gtcatcgttt tggttttggc
     4801 actgatgatt ctgtttgcca aatccaaact ggtcagcgaa ggcgacatca ccatcaaagt
     4861 caacggcgaa aaagagctga cgatgcccgc cggcggcaaa ctcttgggcg cgcttgccaa
     4921 cgaaggcatc tttatcccct ccgcctgcgg cgggggcggt tcgtgcggac aatgccgcgt
     4981 cgtcgtaaaa agcggcggcg gcgacatcct gccgaccgag ctgtcccaca tcagcaaacg
     5041 cgaagcacgc gaaggctgcc gtctgtcgtg ccaagtcaac gtcaaaaccg acatggacat
     5101 cgaagtcccc gaagaagtgt tcggcgtgaa aaaatgggaa tgcaccgtca tctccaacga
     5161 taacaaagcc acgttcatta aagaactcaa gcttgccatt cccgaaggcg aagaagtccc
     5221 cttccgcgcc ggcggctaca ttcaaattga agccccgccg cacaccgttg cctacaaaga
     5281 cttcgacatt cccgaggaat accacgaaga ctgggacaaa tacaatttgt ggcaatacgt
     5341 ttccaaagtg gacgagccga ttttgcgtgc ctactctatg gcttcgtatc ctgaagaaaa
     5401 aggcatcatt atgctgaacg tgcgtatcgc cacgccgccc ccgcgcgtac ctgatgcgcc
     5461 tccgggacaa atgtcgtcct acatctggtc gctcaaaccc ggcgacaaag ttacgatttc
     5521 cggcccgttc ggcgaatttt tcgccaaaga caccgatgcc gaaatggtat ttatcggcgg
     5581 cggtgcgggt atggctccga tgcgttccca cattttcgac cagttgaaac gtttgaactc
     5641 caaacgtaag attacctttt ggtacggcgc acgttccaaa cgcgagatgt tctatgtcga
     5701 agacttcgac caactcgcag cagaattccc gaacttcacg tggcacgtcg ccctgtccga
     5761 cccgctgccg gaagacaact gggacggcta cacgggcttc atccacaacg tggtttacga
     5821 aaaccatctg aaaaaccacg aagcaccgga agactgcgaa ttttatatgt gcggtccgcc
     5881 aatcatgaac cagtccgtga tcaaaatgct caaagacttg ggcgtggaag acgaaaacat
     5941 cctcttggat gatttcggcg gttgagtatt cggcttcaaa tacaaaagga agaaacctcg
     6001 gtttcttcct ttttctatgc cgatgccatc cgaacaccct tcagacggca tcggcataga
     6061 aacataccgg ccgttttgcg atataattca aaccatttgc caaaaccgtc aaacactatg
     6121 ccgtctgaaa cacgcctgcc gaactttatc cgcaccttga tatttgccct gagttttatc
     6181 ttcctgaacg cctgttcgga acaaaccgcg caaaccgtta ccctgcaagg tgaaacgatg
     6241 ggcacgacct ataccgtcaa atacctttca aacaatcggg acaaactccc ctcccctgcc
     6301 gaaatacaaa agcgcatcga tgacgcgctt aaagaagtca accggcagat gtccacctat
     6361 cagcctaatt ccgaaatcag ccggttcaac caaaccacgg ccggcaaacc cctccgcatt
     6421 tcaagcgact tcgcacacgt tactgccgaa gctgtccgcc tgaaccgcct gacacacggc
     6481 gcgctggacg taaccgtcgg ccccttggtc aacctttggg gattcggccc cgacaaatcc
     6541 gttacccgtg aaccgtcgcc ggaacaaatc aaacaggcgg catcttatac gggcatagac
     6601 aaaatcattt tgaaacaagg caaagattac gcttccttga gcaaaaccca ccccaaggcc
     6661 tatttggatt tatcttcgat tgccaaaggc ttcggcgttg ataaagttgc gggcgaactg
     6721 gaaaaatacg gcattcaaaa ttatctggtc gaaatcggcg gcgagttgca cggcaaaggc
     6781 aaaaacgcgc gcggcgaacc gtggcgcatc ggtatcgagc agcccaatat cgtccaaggc
     6841 ggcaatacgc agattatcgt cccgctgaac aaccgttcgc ttgccacttc cggcgattac
     6901 cgtattttcc acgtcgataa aaacggcaaa cgcctctccc atatcattaa tccgaacaac
     6961 aaacgaccca tcagccacaa cctcgcctcc atcagcgtgg tcgcagacag tgcgatgacg
     7021 gcggacggct tgtccacagg attattcgta ttgggcgaaa ccgaagcctt aaagctggca
     7081 gagcgcgaaa aactcgctgt tttcctgatt gtcagggata aaggcggcta ccgcaccgcc
     7141 atgtcttccg aatttgaaaa actgctccgc taaacataca aaccgattag gaaacaccat
     7201 gaaaaccctg ctccttacct tcggcatctt cctgaccgtc atcatcggta tggcggtcgg
     7261 ctatattttc tccaaacgca gcatcaaagg cagttgcggc ggcattactg ccttgggtat
     7321 gaaaaaaatg tgcgactgcg acacaccttg cgacaccctg caaaagaaac tggatgaaga
     7381 aaaacaggca ggcggcatac gggttgaccg ttaacgccga aacaaaatgc cgtctgaagc
     7441 ccgacaggcg ggttcagacg gcattgccgc tttacttcga tcggtttata cgcaaaacgc
     7501 gcttcaggaa ataaatcaga ttattcccca gacgccttca ggcttccttc tgtgccaccg
     7561 taaccatagc cgggcgcaac acgcggtcgg acagcgtata acccttcttc atcacgccca
     7621 ccacggtatt gggttcctgt tcgcttgcca ccgcctgcat cgcctgatgg atattcggat
     7681 cgagcttatc gcccgcttta ggattgattt ccttgatttg cgtagcatca aatgctttct
     7741 gcaactcgtt caaagtcatc tgcacgccca ttttcagcgc atcgaaattg ccgctctgat
     7801 ccaaaagcgc catttccaga taatccttga ccggcagcat ttccacggca aacttctgtc
     7861 cggcgaactt gtgcgtatcc gcaatttcct gctgatggcg gcggcgcagg ttttgctcgt
     7921 ttgccaaagc gcgcagctgc tcgtctttca actgcgcttc cagctcggca atccgcgcct
     7981 gcaaatcctc ataagccggc tctgcggcag cctgttcctg cacaccgtcc gcatttccta
     8041 ctgtctcgac ggtttccacc gcctccacat tttcaaccgc ttcttcactg ttttgctgct
     8101 gtgtctgttc gctcatatcg tatcccttaa aacaaattgg aaatcaaaat ccagttatta
     8161 cccgcgcaag ataaggacat tttcaagaaa cttcaagcaa aggcagagaa tttcaaattc
     8221 attttcaaat cccctaccga aaaaataata tagacggtaa gatactgccg accatatcga
     8281 ttttcaaacg tcatcactca tgaaaaaaga ccatttaaac acaaccggtt tcgacctctg
     8341 gcacaccatc cgcgaagaaa ccgcggccgc ggtctccgcc gaaccgatgc tggcaagttt
     8401 tttgcaccaa accgtgttgc gccacgagtc cctcggctcc gtccttgcct accacctttc
     8461 cagcaaactc ggcagcccga ttatggacgt gcgcgcgctg tttgaaatct accagcaggc
     8521 gttgggcagc gacacccaaa tcggcaaatg cgtcgaagca gacttaaaag ccatctacga
     8581 acgcgatccc gcctgcgacg aatattcgct gccgctttta tatttcaaag gcttccacgc
     8641 gattcaggca caccgcatca accatcggct gtatctcgac gggcgcaaaa cgctggcgta
     8701 tttcctgcaa aaccgtatgt ccgaagtatt cggcgtggac atccatcccg ccgcccgttt
     8761 cggatacggg ctgatgctcg accacgccac cggctttgtt gccggcgaaa ccgccgtgtt
     8821 gggcaacaat atttcgattt tgcacggcgt aacgctcggc ggttcgggca aagaaggcgg
     8881 cgaccgccac cctaaaatcg gcgacggcgt gatgatcggc gcaaacgcct cgatattggg
     8941 caatatccgc atcggcagca atgccaaaat cggggcgggc agcgtcgtgg tttcagacgt
     9001 gccgccgtcc atcacggttg tcggcgtacc cgccaaaccc gtggcgcgat cgctcaaaac
     9061 accgtcggcg gatatggatc aaaatatcca gtttaccgaa atcgacttta tgatttgaat
     9121 atccgcacca atgccgtctg aaacgccaaa cgggcttcag acgacattgc ccgcttcaag
     9181 cctgcgcctt gatatgccgc cgatgtcggc acttgccagt cggacaaaat gccttttatt
     9241 cgatacaccc gattcccacg caaccggaaa tatagtggat taacaaaaat caggacaagg
     9301 cgacgaagcc gcagacagta cggatagtac ggaa
//
LOCUS       DAWKOG010000071         9323 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.071, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000071 DAWKOG010000000
VERSION     DAWKOG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9323
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.071"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..143)
                     /locus_tag="WJK66_001246"
     CDS             complement(<1..143)
                     /locus_tag="WJK66_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578961.1"
                     /translation="MPSERLSDGIFPCVLNRIHETPDAFGGNVGCRAMFGGDQRTKYA
                     GKGL"
     gene            complement(161..718)
                     /locus_tag="WJK66_001247"
     CDS             complement(161..718)
                     /locus_tag="WJK66_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ7578962.1"
                     /translation="MDALKLLTNRRSSKKLKPPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFIVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMSPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDVEAFVSHW"
     gene            complement(794..2428)
                     /locus_tag="WJK66_001248"
     CDS             complement(794..2428)
                     /locus_tag="WJK66_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase LptA"
                     /protein_id="HEZ7578963.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPVGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(2502..2831)
                     /locus_tag="WJK66_001249"
     CDS             complement(2502..2831)
                     /locus_tag="WJK66_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578964.1"
                     /translation="MSRCCLKRLQTASAVSAFSGGVLEVVRLCCRRLSVLFNPGVMLF
                     VPDCPVGGAAGFFQYEMLCPFFWQGRLQTGSNLAYDVFMSAGRLNGGRNPPHSRRFLA
                     LLCSVAL"
     gene            complement(2831..3937)
                     /gene="serC"
                     /locus_tag="WJK66_001250"
     CDS             complement(2831..3937)
                     /gene="serC"
                     /locus_tag="WJK66_001250"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEZ7578965.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSEGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNREGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTGDEELDRRFVLEAELQGLCLLKGY
                     KTVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4302..4733
                     /gene="rimP"
                     /locus_tag="WJK66_001251"
     CDS             4302..4733
                     /gene="rimP"
                     /locus_tag="WJK66_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEZ7578966.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKEGGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            4761..6263
                     /gene="nusA"
                     /locus_tag="WJK66_001252"
     CDS             4761..6263
                     /gene="nusA"
                     /locus_tag="WJK66_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEZ7578967.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6275..9163
                     /gene="infB"
                     /locus_tag="WJK66_001253"
     CDS             6275..9163
                     /gene="infB"
                     /locus_tag="WJK66_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEZ7578968.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAEKAQADKMPSKKPVEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKAAQEAKTGRQRPAKPAEKPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGSKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRAKVVQGEAGGITQHIGAYHVET
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPI
                     VVAVNKIDKDTANPERIRQELTQHEVIPDDWGGTVQFIDVSAKKGTNIDALLEAVLLE
                     AEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMS
                     GMLSPEEKEQVTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHVRLIRNNVVIHTGE
                     LASLKRYKDDVKEVRMGFECGLMIKGYNEIMEGDQLECFDIVEVARTL"
BASE COUNT         2499 a         2689 c         2359 g         1776 t
ORIGIN      
        1 agacctttgc ctgcgtactt ggtgcgctgg tcgcctccga acatggcgcg acacccgaca
       61 tttccgccga acgcatcggg cgtttcatga atccggttta aaacgcatgg aaaaatgccg
      121 tctgaaagcc tttcagacgg cattttgctt gagattccgt ttaccaatga ctgacaaacg
      181 cttccacatc ggtattcttg ggcttatgca cttcctctgt cggcgtgccg accatcatca
      241 gcccgatgat tttatcctta tccgcacatc cgaaagcctc acgcaacagg gggctgttga
      301 cccacatccc cgtaatccag acattgtcga aaccttgcgc cgttgccgcc agttgcagcg
      361 catacgccgc acaacccgcc gtcagcatct gctcccattc cggtttcggc ttaggcacat
      421 cgcggttcgg cgcaaacgtt accccgataa ccatcggcga catattgccc actttttccg
      481 cctttttcat cgcatcgtcg ccgaaattca attcggcaac cgtttgcttt aacacatcgc
      541 gaaaacgttg caatcctacc tcgccttgaa tcacgataaa acggaagggg cgcatattgc
      601 cgtgatcggg aacttgggtt gccgcctgaa atatttgttc caactccgcc gcatcggggg
      661 cggggggctt cagctttttg gaagatcggc ggttcgtcaa taattttaaa gcatccatat
      721 cgttatactc cggtcatcgg tcgggtgttc ggacacatat gccgtccgaa ggcttcagac
      781 ggcatatccg gcatcagcgc ggacggcggc aggctgccaa tatatccatt tccttccgat
      841 aggtttgact gttggaaatg tccatcagcc ccaataccgt gctgaaatag tggtcgtgcg
      901 aatattcgtt ttccgccgct ttttgtttga ggcattggaa atctatgccc ccgtgttggc
      961 ggaaggcttt ggaaaaccac ataaccatcg ggatatgcgt ctgcccggaa ggcgcgatgg
     1021 cgtaaggcgc ggcgtgcagg tacatcccgt tttcgcccaa actttcgccg tggtcggaaa
     1081 cataatgcac cacgctttcc aaatcgtcgc ggttttcaag tttgcggata accttgtcga
     1141 taaactggtc cacatacaaa accgtattgt cgtaagtgtt gaccagcgtg gcgcgggtgc
     1201 atttgttgat ttcgttggtg tcgcaggtcg gcgtgaattt gcgttcggct tcggtatagc
     1261 gttcgtaata cgtcggcccg tggctgccga tggtatgcag gattaaaacc gcgtctttat
     1321 cgtttttgtt gaggacttcg tcgaacttag tcagcaggat attgtcgagg cactcgccgt
     1381 tgcggcagta ttcgggcagg ttgagcgagg taacgtcggt attcggcact ttgccgcaca
     1441 cgcccttgca gccggaatcg ttttccaacc aagtaacttc cacgccggcg cgctgcacga
     1501 tgtccagcag gttgtcttgg tgttcggctt tgatttcgtc ataatccgtg cggtcgaagg
     1561 ttgagaacat acacggcagg gagtgcgcgg tcgatgtgcc gcagcttctg acctgcggga
     1621 aattgacaat ttcatcgccg cgcgcggcaa gcagcggcgt agtttggcgg ctgtaaccgt
     1681 tcaaacccca gttggcggca cgcgtggtct cgcccacgac cagcaccacg aaacggcgca
     1741 ggctgccgac cgggcggttt tgcacgaccg ccatatccaa ttgcgtataa ggaatattgg
     1801 aacgcttcca atctttgtat ttcgacacgc ccgcgccgat gaaattagac ggcacaatca
     1861 gatgggttac tgatttattg ttgcggaaaa acgaggcgta atcctgatat tgcaacattg
     1921 cgatgcccaa cgcgcacaaa aaggaaacgg cggcaagcac aaggcgcgtc aaaagctcct
     1981 tataccaaac gcggtattta accttgacgg cgatatacgc cagcgcgggc aatacgccca
     2041 aacatacaat ccacagcaca tagcccggcg taatcaggcg cgcgctttcc gccgccgtgg
     2101 tttgcaagac attattcaac atcgacttgt tgaaatagat attgaaaaat atttcttggt
     2161 aagacaccgc cgcactgata accaatatca acggaatcaa taccttatgc acgaaaggca
     2221 aggcaatgac gtgaaaaacg aaattactta aaaaaaacag caccaccggc atcgtataga
     2281 ggaagatatc cgccccggtg ccgttaaaag gatgaagctc gacaactttg gcaaaaaagg
     2341 cgtaattcaa taccagcgag gaatacaggg aaaggaaggc aatcagcgcg gaagagccga
     2401 gcttcggcct caggttcggt tttatcattt ggaatgtgtc ggataagggt tggaaaaggc
     2461 atccggcatt tggaatccgg attattgaaa aagattctta attataaggc aacggagcaa
     2521 agcagggcaa gaaaacggcg gctgtgcggg ggattccgcc cgccattcaa acgtccggca
     2581 gacataaaaa catcgtaagc aagattcgaa ccggtctgca accgcccctg ccagaaaaac
     2641 gggcaaagca tttcatattg gaaaaacccg gccgcgccgc cgacgggaca gtccggcaca
     2701 aacagcatca cgcccggatt gaaaaggacg gatagccgcc ggcagcacaa tcttaccacc
     2761 tccaaaacgc cgccggagaa cgccgaaaca gccgatgccg tctgaagccg cttcagacaa
     2821 catcgggaca tcaaccgtaa cgccgttgga aatcgcgcat aaaatccgcc aaagcccgca
     2881 cgccttcaag cggcatcgca ttataaatgc tggcacgcat accgccgacg gttttatagc
     2941 ccttaagcag gcacaagccc tgcaattcgg cttccagcac aaaacggcgg tcaagctcct
     3001 catcccccgt ttggaacacg acattcattt tagaacgcgc attcggacgg atacggttga
     3061 tatagaaacc gccgctgccg tctatcgtct catacaaggt ttgcgccttc agccgattga
     3121 ccgcttcaat ttttttcaca ccgccctgcg cctgcagcca gcggaacacc agccccgaca
     3181 tataaatcgc gtaagttgac ggcgtgttgt acataccttc gcggttcaaa tgcgaacggt
     3241 agttgaacac atcgggaata tcgttcggac aacgctcgag caaatcctca cgcacaatca
     3301 ccaccgtaac ccctgccggc ccgatgtttt tctgtgcgcc tgcgtaaatc agtccgtagt
     3361 cggcaacatc aaactcgcgc gacaaaatct cgctggacat atcgcacacc agcggcggca
     3421 tgccttctga aaggcgcggc acttcacggt attgcagccc gttgaccgtt tcattgacgg
     3481 caaaatggac aaacgccgaa tcgggtgcaa catcccacgt ttccacaggc ggcaggtcga
     3541 gatagtcgaa ctgctcgccg ccatgcgccg ccaaacggat ttccgtatcg gtcaaacggc
     3601 tcatctgttc ataagcgata cggctccagt tgcccgttac caccgcgtcg gcagtgcgga
     3661 aaccgtgtgc cagattcatg gctgccatat taaattgggt tgttgctccg ccctgcagaa
     3721 acaatatctt atagttgtca ggcactttca aaagctgcct caaatcctgt tccgcatgat
     3781 gcaggatgct caaaaacatt tccgaacggt ggctcattgc catcacagga aaacccgtac
     3841 cgttgtagtc caacatttcc tgccgcgccg tttccaacac ggcttcgggc aatacggcag
     3901 ggccggcgga aaaattgtaa atcggataaa gagacatgat gcagccttga ttctgaacaa
     3961 taacccgccc gattttaggc ttgcagcagg ctttgtgcaa ggatggaaaa tacctgtcct
     4021 ccgccccgct tcatgccgcc cgaacacaga aaacaacatc aatatattga tttacaaaca
     4081 taaaaatcat gcacgcgaca aatagataca tttgttttgt caacaatatt cacgatttcc
     4141 cattacaaac ctcccttaca tccgcttttt tccgtcccaa aaacacaaaa taaatcaaca
     4201 ctttcatttc tccgcaaaag cggttataat tacgccgatt tttcaacttt gacgaaaaat
     4261 ggccacgggg ccattttttt attatgtaca tagggagcag catggatatc caaaccatcc
     4321 tcgaaaaaac cctgcccggc ctgggctacg aactggtcga ttttgaactg accgcgcaag
     4381 gaacattgcg cgtgttcatc gacaaagaag gcggcattac cgtcgaagac tgcgcaaccg
     4441 tcagcaacca cttgagccgc gtcttcatgg ttgaagacat cgactacaaa aacctggaaa
     4501 tttccagccc cggactcgac cgccccttga aaaaagccgc cgacttcgtg cgctttgccg
     4561 gtcagaatgc caaaatcaaa acccgcctgc cgatagacgg tcaaaaaaac tttatcggta
     4621 aaatcgaagg ctgcgaaaac gacactgtta ccgtttcctt cgacggcaaa accgtacaaa
     4681 tcgaattggg caacatcgac aaagcccgcc tgcgccccga attcaaattc taaaacacaa
     4741 caatattgga gatgttcaaa atgagtcgtg aaatgttaca gctggcagaa gcactggcaa
     4801 gcgaaaaaaa cgttgatgcg gaagtcgtct tccaagcact ggaattcgcc ctgtctaccg
     4861 ccgccaagaa aaaggcagac cgcgaacaca tggacgtgcg cgtccaaatc aaccgcgaca
     4921 ccggcgaata ccaaaccttc cgccgctggc tgattgtcgc cgatgaagac tatacctatc
     4981 ccgatgtcga aaaaaccatc gaggaaatcc aagaggaaat tcccggcact accatccaaa
     5041 tcggcgaata ctacgaagag cagctaccca acgaaggctt cggccgccaa gccgcgcaaa
     5101 ccgccaaaca aatcatcctg caacgcatcc gcgatgcgga gcgcgagcaa aatctgaacg
     5161 agtttctcgc cgtcaaagaa gacatcgtgt ccggcacggt caaacgcgtc gaacgccacg
     5221 gcatcatcgt cgaagtcgtt gccggcaaac tggacgcgct gattccgcgc gaccaaatga
     5281 ttccgcgcga aaacttccgc agcggcgacc gcatccgcgc cctcttcctg cgcgtcgaag
     5341 aaatcggcaa caccggccgc aaacaagtca tcctgagccg tacttccggc gatttcctcg
     5401 tcaaactgta cgccaatgaa gtacctgaaa ttgcagacgg catgcttgaa atccgcgctg
     5461 tcgcccgcga cccgggacaa cgtgccaaag tcgccgtcaa agccaacgac cagcgcatcg
     5521 atccgcaagg cacctgtatc ggcgttcgcg gttcgcgtgt caatgccgtc agcaacgaat
     5581 tgtccggcga gcgcatcgac gttgtccttt ggtcgcccga gcctgcccaa ttcgtcatga
     5641 gcgcgctctc acccgccgaa gtcagccgca tcgtcatcga cgaagacaaa cacgccgtcg
     5701 atgtcatcgt tgccgaagac cagctcgcgc tcgccatcgg acgcggcggt caaaacgtgc
     5761 gccttgcttc cgacctgacc ggctggcagc tcaacatcat gacttccgcc gaggcagacg
     5821 aacgcaatgc ggcagaagat gccgccatcc gccgcctgtt tatggatcac ttgaacgtgg
     5881 acgaagaaac cgccgacgta ctggttcagg aaggttttgc aaccttggaa gaagtcgcct
     5941 atgttcctgc cgccgaactg cttgccattg aaggatttga cgaagaaatc gtcgatatgc
     6001 tccgcaaccg cgcccgcgat gccatcctga ccatggcgat tgccgccgaa gaaaaactgg
     6061 gcgaagtatc cgacgatatg cgcaacctcg aaggcataga tgccgatatg ctccgcagcc
     6121 ttgccgaagc aggcattacc acccgcgacg acttggcaga gcttgctgtg gacgaactga
     6181 ttgaaatcac cggtgtaaac gaagaaaccg caaaagccgt catcctgacc gcacgcgaac
     6241 actggtttac cgaagacaaa taaagggggt acagatgagt aacacaaccg tagaacaatt
     6301 tgccgccgag ctgaaacgcc ccgtcgaaga cctgttgaaa cagttgaaag aagccggcgt
     6361 cagcaaaaac agcggcagcg attccctgac gctggacgac aaacagcttc tgaacgccta
     6421 cctgaccaag aaaaacggca gcaacggcgg caccatcagc atccgccgca ccaaaaccga
     6481 agtcagcacc gttgacggcg taaaagtcga aacacgcaaa cgcggacgca ctgtcaacat
     6541 tccttctgcc gaagaattgg cagcacaggt aaaagccgcc caaacccaag ccgcacctgt
     6601 ccggccggag cagacggcag aagacgcggc aaaagcccga gccgaagctg ccgcacgtgc
     6661 agaagcccgt gccaaggcag aagcggaagc ggcaaaactg aaagcggcaa aagcaggcaa
     6721 caaagccaaa cctgccgcgc agaaacccac cgaagcaaaa gccgaaaccg cacccgttgc
     6781 ggcggaaacc aaacccgccg aagaaagcaa agcggaaaaa gcccaagccg acaaaatgcc
     6841 gtctaaaaaa cccgtcgagc ccaaagaaaa agccgccaag ccgaaacacg agcgaaacgg
     6901 caaaggcaaa gatgccaaaa aaccggcgaa acctgccgca cctgccgtgc cgcaacccgt
     6961 ggtcagcgcg gaagaacagg cgcaacgcga tgaagaagca cgccgtgccg ccgcacttcg
     7021 cgcccaccag gaagccctgt tgaaagagaa acaggaacgc caggcacgcc gcgaagccat
     7081 gaaacaacag gcagaacaac aggcaaaagc cgcacaggaa gccaaaaccg gcagacagcg
     7141 tcccgccaaa cctgccgaga aaccgcaggc agccgcgccg gccgtcgaaa acaaacctgt
     7201 caatccggca aaagcgaaaa aagaagaccg ccgcaaccgc gatgacgaag gtcaaggccg
     7261 caacgccaaa ggcaaaggcg gaaaaggcgg gcgcgaccgc aacaatgcac gcaacggcga
     7321 cgacgagcgc gtacgcggca gcaaaaaagg caaaaaactc aaactcgagc cgaatcaaca
     7381 cgccttccaa gcaccgaccg aacccgtcgt tcatgaagtt ttggttcccg aaaccattac
     7441 cgttgccgat ttggcgcaca aaatggcggt caaaggcgtg gaagtggtca aagccctgat
     7501 gaagatgggc atgatggtaa ccatcaacca atccatcgac caagacaccg ccctgattgt
     7561 cgtcgaagaa ctcggacaca tcggcaaacc cgccgccgcc gacgatcccg aagccttctt
     7621 ggacgagggc gcggaagcgg tggaagccga agcattgccg cgtccgcccg tcgttaccgt
     7681 catgggacac gtcgaccacg gcaaaacctc gctgctggac tacatccgcc gtgccaaagt
     7741 ggtacagggc gaagcgggcg gcattaccca acacatcggt gcataccacg ttgaaacccc
     7801 acgcggcgtg attaccttct tggatactcc gggtcacgaa gcgtttaccg ccatgcgcgc
     7861 gcgcggcgcg aaagcgaccg acatcgtgat tctcgtggtt gccgcagacg acggtgtaat
     7921 gccgcaaacc atcgaagcca ttgcacacgc caaagcggca ggcgttccga ttgtggttgc
     7981 cgtcaacaaa atcgataaag acactgccaa ccccgaacgc atccgccagg aactgaccca
     8041 acacgaagtg atccccgatg attggggcgg cacggttcaa ttcatcgacg tttctgcgaa
     8101 aaaaggaaca aacatcgacg cgctgctcga agcagtcttg ctcgaagccg aagtattgga
     8161 actgaccgca cctgtcgatg cgcccgccaa aggcatcatc gtcgaggcgc gcttggacaa
     8221 aggccgcggc gcggttgcca cattgctggt tcaaagcggt acgctgaaaa aaggcgacat
     8281 gctgctggcc ggtacggcat tcggcaaaat ccgcgcgatg gtcgatgaaa acggcaaatc
     8341 cattaccgaa gccggcccgt ccatccccgt cgaaatcctc ggcttgtccg acgtaccgaa
     8401 tgcgggtgaa gacgcgatgg tattggcgga cgagaaaaaa gcgcgcgaaa tcgccctctt
     8461 ccgccaaggc aaataccgcg acgtgcgcct tgccaaacag caggcggcga agctggaaaa
     8521 tatgttcaac aatatgggcg aaacccaggc ccaatctttg tcggtcatca tcaaggcaga
     8581 cgtgcagggc tcttacgagg ctttggcggg cagcctgaaa aaactgtcca cagacgaagt
     8641 gaaagtgaac gtgttgcaca gcggcgtggg cggcattacc gaatcggatg tcaaccttgc
     8701 catcgcttcg ggcgcattca ttatcggctt taacgtgcgt gcagatgcct cttcgcgcaa
     8761 acttgccgaa aatgaaaacg tggaaatccg ctactacaac atcatctatg atgccatcga
     8821 cgacgtgaaa gcggcgatga gcggtatgct ttctcccgaa gagaaagaac aggttaccgg
     8881 tacggtcgaa atccgtcagg ttatctccgt ttccaaagtc ggcaatattg caggctgtat
     8941 ggttaccgac ggcgtggtca aacgcgattc ccatgtccgc ctcatccgca acaacgtggt
     9001 tatccacacg ggcgaactgg cttcgttgaa acgctataaa gacgatgtaa aagaagtccg
     9061 catgggcttc gagtgcggcc tgatgattaa aggctacaac gaaattatgg aaggcgacca
     9121 actcgaatgt ttcgacatcg tcgaagtggc acgcaccctg tgattttgat gcggaaaaat
     9181 gccgtctgaa gcgttcagac ggcatacgaa acgggttctg tatcatacag aacccgtttt
     9241 ttgtcgcaaa tcggcttcag acagccctct tgccttatcc cgatttgaat ctgacttgcc
     9301 atacaaacag gcttcagacg gca
//
LOCUS       DAWKOG010000072         9272 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.072, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000072 DAWKOG010000000
VERSION     DAWKOG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9272
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.072"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            88..297
                     /locus_tag="WJK66_001254"
     CDS             88..297
                     /locus_tag="WJK66_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEZ7578969.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            386..1600
                     /locus_tag="WJK66_001255"
     CDS             386..1600
                     /locus_tag="WJK66_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEZ7578970.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVDDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKSYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVSQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(1687..4266)
                     /gene="clpB"
                     /locus_tag="WJK66_001256"
     CDS             complement(1687..4266)
                     /gene="clpB"
                     /locus_tag="WJK66_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEZ7578971.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADGSYLEAGFVLKALLDDQNS
                     GAAALLAHSGVNVPQVKQRLQQHLNSLPKVSSQGGDILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDATGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEING
                     LQKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGTQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQANIRSIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEANGDRL
                     KFA"
     gene            4526..5536
                     /gene="trpS"
                     /locus_tag="WJK66_001257"
     CDS             4526..5536
                     /gene="trpS"
                     /locus_tag="WJK66_001257"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEZ7578972.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANSENNQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFKEIFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETAAFTQMLAEGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            5542..5883
                     /locus_tag="WJK66_001258"
     CDS             5542..5883
                     /locus_tag="WJK66_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578973.1"
                     /translation="MPSEPLKGLPEQNVVRLIGKHPNDLEAVVGKCMETDGKGAPSGW
                     AANGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQNGRYVLEIDS
                     EGAFYFRRRHY"
     gene            6205..6528
                     /locus_tag="WJK66_001259"
     CDS             6205..6528
                     /locus_tag="WJK66_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578974.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEVKDQVKDAAADAKASAEEAVTEAKEAVTEAAKDTLNKAADATQEAADKM
                     KDAAK"
     gene            complement(6737..8245)
                     /gene="ppx"
                     /locus_tag="WJK66_001260"
     CDS             complement(6737..8245)
                     /gene="ppx"
                     /locus_tag="WJK66_001260"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEZ7578975.1"
                     /translation="MTTTPANVLASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQAAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALSERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEVV"
     gene            complement(8361..9093)
                     /locus_tag="WJK66_001261"
                     /pseudo
     CDS             complement(8361..9093)
                     /locus_tag="WJK66_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
BASE COUNT         2137 a         2399 c         2450 g         2286 t
ORIGIN      
        1 atttgaattt ttcagaaaat gaagcggacg tttgggcgga ggcaacttgt ttgataagat
       61 agcaatattt caaaacggag aaagatcatg ccttatgtca atattaaagt aaccggcggc
      121 aaggaagcac cgactgccgc gcaaaaagcg gaactgatcg gcggcgtaac cgaattgctg
      181 gcacgcgtgc tgggcaaaaa tcccgaaaca acggttgtcg tgattgatga agtggatacc
      241 gataactggg gaataggcgg caaaagcgtc agcgaacggc gcaaagaggg caggtaaaaa
      301 gcctgaaaat ctcggtttga tgctttaaat tccgcgtgaa aaagagtaca ttcccaccca
      361 ttgcccaaaa tttacggaac acatcatgga taaatttccc aagtctgcaa agctcgatca
      421 cgtctgttac gacatacgcg ggccggttca caaaaaagcc cttcagttgg aagaggaggg
      481 caataaaatc cttaaactca atatcggcaa ccctgcgccg ttcggctttg aagcccctga
      541 tgaaatcttg gtcgatgtca tccgcaacct gccgacttcg caaggctatt gcgattctaa
      601 agggctgtat tccgcccgca aagccattgt tcactactat cagaccaaag gtttgcgcga
      661 tattacggtt gatgatgtct atatcggcaa cggcgtgtcc gagctgatta cgatgtctat
      721 gcaggcattg ctcaacgacg gcgacgaaat cctgattccc gcgcccgact accctttgtg
      781 gacggcggcg gcaacgcttg cgggcggtac ggtacgccat tatctgtgcg acgaagaaaa
      841 cggctggttc cccaaccttg ccgatatgga agccaaaatc acgcccaaaa ccaaagccat
      901 cgtcgtcatc aatcccaata atccgacagg cgcggtgtac agcagggaaa tcctgttgga
      961 aatcgccgaa ctggcgcgca agcacggttt gattattttc gccgacgaga tttacgataa
     1021 aatcctttat gacggcgcgg ttcaccacca catcgccgcg cttgcccccg accttttgac
     1081 ggtaacgttc aacggtttgt ccaaatccta ccgcgtcgcc ggcttccgcc aaggctggat
     1141 ggtgctcaac gggccgaaac atcacgcaaa aggttacatc gagggtttgg atatgctctc
     1201 gtctatgcgc ctgtgtgcca atacgccgat gcagcacgcg attcagacgg cattgggcgg
     1261 ctatcagagc atcaacgaat ttattttgcc cggcgggcgg cttttggaac agcgcaacag
     1321 ggcgtgggaa ctggtcagcc aaatccccgg cgtgtcctgc gtcaaaccga tgggtgcgat
     1381 gtatatgttc ccgaaaatcg ataccgaaat gtaccgcatc cgcgatgaca tgaaattcgt
     1441 ttacgatttg ctggtgcgcg aaaaagtctt gctggtacag ggaacggggt ttaattggat
     1501 caagcccgac catttccgca ttgttacgct gccttacgtc catcagattg aagaggcgat
     1561 gggcaggctg gcaagattcc tgcaaaccta ccgccaataa ggggacggtt tgtctgccga
     1621 ggataaaaaa tgccgtctga aacggagatt cccgtttcag acggcatttt caacagcagg
     1681 aacgaatcag gcaaatttca gtctgtcgcc gttggcttct accctgattt cgctttcggg
     1741 cgcatagttt ccggcaagca gagcttttgc cagcgggttt tcgatttccg actggatggc
     1801 gcgtttgagc ggacgtgcgc cgtaaatcgg gtcgaaaccg gctttggcga tgatgtccag
     1861 agcggcatcg gaaacagtca ggcggaggtt ttgtttttcc aaacgttttt ccaaaccttt
     1921 aagctggatt ttggcaatac tgcggatgtt tgcctgatcc agtccgtgga acacgaccac
     1981 ttcgtcgatg cggttgatca tttcaggacg gaaatgttct ttcacatcct ccatcacaac
     2041 ttctttcacc gcttcgtagt cttgagtacc catttgttgg atgtgctggc tgccgatgtt
     2101 ggaagtcatc acaatcacgg tatttttaaa atctaccgtc ctgccttgtc cgtcggtcaa
     2161 gcggccgtca tccaatactt gcagcaggat gttgaacaca tcgggatggg ctttttccac
     2221 ttcgtccaaa agaattacgc tgtacggttt gcggcggact tgttcggtca ggtagccgcc
     2281 ttcttcgtag ccgacatagc ctggaggcgc gccgatgagg cgggcaacgg cgtgtttttc
     2341 catatattcg gacatatcga tacgaatcag atgatcttca ctgtcaaaca ggaagcctgc
     2401 caatgctttg cacaactcgg ttttacccac gccggtcggg cccaagaaca ggaagctgcc
     2461 gtaaggttta ttcgggtcgg caagaccgga gcggctgcgg cggatggcgt cggacacggc
     2521 acgcacggct tcgtcttgac cgactacgcg gcggtgcaac acttcttcca ttttcagcaa
     2581 cttgtcgcgt tcgccttcca tcatttttga cacaggaatg ccggtcatac gggaaaccac
     2641 ttctgcgatt tcctctgcgc cgacattatt acgcaagagt ttgtttgccg gttttgtgct
     2701 gtccgtatct gcccgttcgg cggctgcacg ctgtttttcc aaatgctcca aatcttcata
     2761 catcaatttt gaagccagtg ccaaatcgcc ttgccgtttt gcctgttcga ttttaatttt
     2821 gacttcgtca atttgtttct taatattagc agcaccgtct gaaattgctt tttcggcttt
     2881 ccagatttcg tctaaatcgg cgtattcttt ttgcagaccg ttgatttcct cgtctatcag
     2941 ttccaaacgt tttttgctgg catcgtcttt ttctttttca acgtgcgcct tttccatccg
     3001 aagctgaatt agacggcggt cgattttgtc cattgcttcc ggcttggttt ctttttccat
     3061 cttgacacgg ctggcggctt cgtcaatcaa atcaatcgct ttatcgggca ggaagcggtc
     3121 ggtgatgtag cggtcgctca actctgcggc ggcaacaatg gcggggtcgg tgatgtcgat
     3181 gccgtggtgg atttcgtagc gttcctgcaa accgcgcaag atggcgatgg tgtcttccac
     3241 actgggttcg ccaaccaata ctttttggaa acggcgttcg agtgccgcgt ctttttcgat
     3301 gtattggcgg tattcgtcca aagtagttgc accgatacag tgcaattcgc cgcgtgccaa
     3361 agccggtttc aacatattac ccgcgtccat cgcgccgtcg gttttacctg caccaaccaa
     3421 agtatgaatt tcgtcaatga aaatcagagt gttgccatcg tctttagcca agtcgttcaa
     3481 cacgcctttc aagcgttctt caaattcgcc gcggtatttc gcgccggcaa tcaatgccgc
     3541 caagtccaaa accagtaggc gtttgttgcg cagggattca ggcacttcgc cgttgacgat
     3601 acgttgcgcc aagccttcaa caatggcggt tttacccacg cccggttcgc caatcagcac
     3661 ggggttgttt ttggtgcggc gttgcaatac ctgaattgca cggcggattt cgtcgtcacg
     3721 accgataacg gggtcgagct taccgtctcg ggcgcgttgg gtcagatcga gcgtgtattt
     3781 tttcagcgca tcgcgttggt cttcggcatt ggcatcgttc acgttttgtc ctcctcgtac
     3841 cgcgtcaatc gcggcattga tgttttgttc ggtcgcgccg gcttctttca agattttgcc
     3901 ggtcgcatcg ttctgctgca ccaaggcgag caggaaaagt tcgctggcga tataggcatc
     3961 gccgcgtttg gttgcggctt tgtccatcag gttcaacacc gcctgcaatt cgcggctggg
     4021 cagaatgtcg ccgccctggc tggacacttt cggcaggctg ttcaaatgct gctgcaaacg
     4081 ctgtttcacc tgcggcacgt tcacgcccga atgcgccagc aacgcggcgg ctccgctgtt
     4141 ttggtcgtca agcagggctt ttaacacaaa gcctgcttcc agatagctgc cgtccgcagc
     4201 caacgccaaa ctctgagctt ctgcaagggc ttgttggaat ttggcggtta atttgtcgta
     4261 tcgcattttt gtttcctttt caaaatgtcc gctgtcgaag cctatatgtg cataattgtg
     4321 gataactcaa gttctgtttt ctgtttttct atatttaatt cgatatatca ttgaatttaa
     4381 agtatataaa aatgtataat aatgtgtata actatatctt cttaatatgg aaaagtctgt
     4441 tgtcggctgg atgtaggtgg caaatcgggt ataatcggca catctttttc cctttcagac
     4501 ggcattgatg ccgcaaggac attttatgag caaaaaacga gttctgaccg gcgtaaccac
     4561 caccggcatc ccgcatctgg gcaactacgt cggtgctatc cgccccgccg tccgcgcggc
     4621 gcaaaaccct gataccgaat ccttcctctt cctcgccgat taccacggta tcatcaaatg
     4681 ccacgagccg gagatgattc accaatccac ccaagccgtt gccgccactt ggcttgcctg
     4741 cggactcgac cccgagcgca ccaccttcta ccgccaaagc gacattcccg aagtgatgga
     4801 attgaactgg attctgacct gtatcaccgc caagggtttg atgaaccgcg cccacgccta
     4861 caaagccgcc gtgcaggcaa attcagaaaa caatcaggaa gatcccgatt tcggcgtgga
     4921 aatgggtttg ttcagctatc cgattctgat gaccgccgac atccttatgt tcaacgccaa
     4981 cgaagtgccc gtcgggcgcg accaaatcca acacgtcgaa atggcgcgcg acatcgccgg
     5041 ccgcttcaat caccgcttta aagaaatctt taccctgccc gaagtgaaaa tcgacgaaaa
     5101 cgtcgaactc ttggtcggtt tggacggacg caaaatgtcc aaatcctacg gcaacaccat
     5161 tccgctttgg gaaaacgaca aaaaaaccca aaaatcggtc aacaaaatca tcaccaatat
     5221 gaaagagccg ggcgagccga aacagcccga cgaaagccca ttgtttgaaa tctacaaagc
     5281 cttctccacg ccgtctgaaa ccgcagcgtt cacgcaaatg cttgccgaag gcttggcttg
     5341 gggcgaggca aaaaaacttt tggcggcgaa aatcaacgcc gaactcgccg aaccgcgcga
     5401 acgctacaac gagctgaccg ccgacccttc gcaaatcgaa gagattttgc aggcaggcgc
     5461 gcaaaaagcg cgtaaagaag cacgcgaatt gttggacaaa gtacgcgatg cggtcggcat
     5521 ccgcccgttg aaataaaccc gatgccgtcc gaacctctta aaggcttgcc ggaacaaaac
     5581 gtcgtccggc tgatcggcaa gcatcccaac gacttggaag ccgtcgtcgg caaatgtatg
     5641 gaaaccgacg gaaagggcgc gccttcgggc tgggcggcaa acggcgtgtg ccataccttg
     5701 tttgccaaac tggtgggcaa tatcgccgaa gacggcggca aactgacgga ttacctgatt
     5761 tcgcattccg ccctgcaacc ctatcaggca ggcaaaagcg gctatgccgc cgtgcagaac
     5821 ggacgctatg tgctggaaat cgacagcgaa ggggcgtttt atttccgccg ccgccattat
     5881 tgaggtattc ggacatcccg gaatatattt gggtttttca aaccctgcgg gaaaaaagtc
     5941 cgcaccgtcg ggaaactcaa aaggaggggg atatgtgtta caattttccg aactgttttc
     6001 ataaaatagt tttcggacgt gtttcaatat ggcattgatg ccgccttatt gttcgagaaa
     6061 aaaaaccttt atatttaaat ataatgggtt ttaactaaac gggaaaccgt tttctctccg
     6121 gccgatgggc aaaatcagcc gattgatgga acacggtccg atttttaagc aaaaccttaa
     6181 tcaacaactc aagaaggata agacatgaaa aaattattga ttgccgcaat gatggcggct
     6241 gccttggcag cttgttcgca agaagccaaa caggaggtta aggaagcggt tcaagccgtt
     6301 gagtccgatg ttaaagacac tgcggcttct gccgccgagt ctgccgcttc tgccgtcgaa
     6361 gaagtgaaag accaagtcaa agatgctgcg gctgatgcaa aggcaagtgc cgaggaagct
     6421 gtaactgaag ccaaagaagc tgtaactgaa gcagctaaag atactttgaa caaagctgcc
     6481 gacgcgactc aggaagcggc agacaaaatg aaagatgccg ccaaataatt tgttgccttg
     6541 gcaaaaatga tgggatgccg cctgccggca acccaaacga aaccgcctga agattttcag
     6601 gcggtttttg agttgtatgc cccttgtttt ttacgcctgc atgaccgttc cgagcagatg
     6661 tgccgcgcat atcgggatgt tgcggacagg caaatgccgt ctggacaggg tttggacggc
     6721 atttgttccg taaggttcag acgacctcga ctttaaacgg catattgatt ttttgccatt
     6781 ggacgctttc atattccaat gcgtcggcaa tcaggggatg gcgttccagc cattccctgt
     6841 caatacgcag gatgaagccg cagctttccg tatccgtgcg caactgcata tttttcggga
     6901 aagacaggtc ttggcgcgaa cggcagaaca gggcagcaag gcgcagggac aaaacggcat
     6961 accacaacat ttcgttggtg ccgatgatgc cgctcatttt tttcatatcg ccgcgatgac
     7021 cgatgaccag ttgggcaagt atggtctgtt ctttgcgtga gaaacccggc atatcggcgt
     7081 tttcgaggat gtaggcggaa tgcttgtgat agccggtgtg ggcgatgtcc aaaccgattt
     7141 cgtgcagcgc ggcggcgcgt ccgagatact gttgccacaa ggcaagctct tgaactgtaa
     7201 cgtttttagc gtggcagagg ctgtccataa aggtttgcgc ggtctcggcg gtgcgtttcg
     7261 cctgattgag gctgacgtgg tagcggtgtt ggaactcggc aaccgtttgt ccgcgcatat
     7321 cttcgtttaa accgcgcccg atcaaatcgt aaaacacgcc gtcgcgcagg gcggcttcgg
     7381 ttacggtcat cctgtcgagt ttcatttcct caaacgccgc catcatcacg gcaagtccgc
     7441 cggcaaaaac ttcgatgcgt tccggtttca ggttttcaaa tttggctttt ttgaccgaac
     7501 cggcttcgat gatgcgttcg gagagggcgc gcataccttt gtaggtaatg tccgcctctt
     7561 ggggcatttc ggcggcaagc acgtcgcgga tggattttgc cgaacccgat gtgccgacgg
     7621 cgaaatccca gccttcgcgc ctcatatttt tgctgatgcg ctggatttcg ttgcgggcgg
     7681 cggaaatggc ggcttggaag tctttggcgg tgattttgtt ttggaagaag cgcaggctgt
     7741 aggttacgca gcccaagggc aggctttcgg taatgtcggg attcagcgtc gaaccgatga
     7801 caaattctgt cgaaccgccc ccgatgtcga taaccagcat tttgccgccg ccggggggga
     7861 gggtgtggat cacgccggta taaatcagcc gtgcctcttc gcgcccggcg atgatttcga
     7921 tggggaaacc cagtgccgct tcggctttgg gaaggaaatc tgcgatgttt ttggcgacac
     7981 ggaatgtatt ggttgccacg gcgcgtacct gttcagggcg gaagccgcgc aggcgttcgc
     8041 cgaattttgc cagacagtcc aaagcctgtt cttgggaagc ggcactcagg tttttctgtt
     8101 cgtccagtcc ggcggcgaag cgcaccatct gtttgaacga atcgatgact ttcaactgtc
     8161 cgttgttgtt ttcgcaaatc tggaggcgga aactgttaga acccaaatcg acggaggcaa
     8221 ggacgtttgc gggggtggtg gtcatggcgg ataccggtgg gggaaaaacg caatgttact
     8281 ctgacggcgc aggcgttgac aataaatgat gcggcggttt ttgattctgc ccacggatgt
     8341 tgccgacggc atttttgcgc ttatttgaaa tccttttcca cgctcatgaa aatctgcgtg
     8401 tttttgcgtg tgtaaaaact tttcatattg ctgtcgattt tcagatagcg gaaattgagt
     8461 tgcggcgtaa agcccttcca agagattttg tcgtgccaca acgacaggtt tgcctgatat
     8521 tcgtggtctt tgcgcgggaa gcggtacaca atggtcccgg gtgcgtcaaa catcctgcgg
     8581 gtatagcgca ggtttgcccg cagacccaag cccgccgtcg aacgttttga ctgcgccgac
     8641 acgcaaaccc ttgcggacgg aagcctgttc cgcctctttc gtcatgttgt gcgaccagtc
     8701 cgcaccgccg taaagcagcc agtctttcgg cgcggaatac atcagcgtcg cgcccgccag
     8761 cggcatatgg ctgtcgtatc gggcggcggt gcggtcttcc tgataacgct tccacatatt
     8821 gcccgcgttt agtgtcaacc gccagcgttc gcccaagcgt tgggagaaat cggcattgaa
     8881 gccgccgacg aaattgtatc ggctgccgcc taagaggttt tgcccgacaa acggcacgat
     8941 gccgaacgag cgcgttaccg aacgattttt atagccgagc gacaggtgca ggcactgttc
     9001 gctgaaatct ttgttatccc aataatgcac gccgccgccg ctgatgccgc cgtagaggaa
     9061 atgatgcccg cccgcattga tttcgcgcga cacgcctaag ccctagcgca aaccgtgtgc
     9121 cttttgcggc aggctgtcgg cggttttcgt ccagcttctg cccgcaaatt caatcgtttt
     9181 tttggacgaa gcgttgttta tagcggatta acaaaaatca ggacaaggcg acgaagcctc
     9241 agacagtaca gatagtacgg aaccgattca ct
//
LOCUS       DAWKOG010000073         9132 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.073, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000073 DAWKOG010000000
VERSION     DAWKOG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9132
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.073"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(72..1328)
                     /locus_tag="WJK66_001262"
     CDS             complement(72..1328)
                     /locus_tag="WJK66_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEZ7578976.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPSDAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEEIEFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWREEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDSRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSILPEENELQVERFLSEHPEFEPVNCAELLAGLKIDL
                     DTGKYLRLNSARHQTDGFFAAVLQRK"
     gene            complement(1676..2017)
                     /locus_tag="WJK66_001263"
     CDS             complement(1676..2017)
                     /locus_tag="WJK66_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578977.1"
                     /translation="MKNNVKNWTTKEVKQSLDKFNNILIKNTFLLQYLKKEFSASSAY
                     CLSMLPEEEDIYEILVNGNIIIDLEFNKHTNETVVINVTDVDEYLKTLTNESGRVFFT
                     LAKEIGKQKNI"
     gene            complement(2221..3078)
                     /locus_tag="WJK66_001264"
     CDS             complement(2221..3078)
                     /locus_tag="WJK66_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HEZ7578978.1"
                     /translation="MNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELGVRELLGE
                     RGFRYLLALDADPAPLTEHLTRRAPFGHRLGIYAEELLAFWFANAPHAELLAHNLTVS
                     GSDGNTQGAADFVVRLNGKPYHIELTCKYYGGGTGRLEDMRGLDPKDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLDVKPVSIVRGIGFFPHGFHAFEPPLNPYGWRGIYIQD
                     WAEYGFKRQEVRYHLLDRMAYLAPARVAETETLNATEIRRIDQGLIAVLECRPDGFWH
                     EIERIMKAV"
     gene            complement(3139..3954)
                     /locus_tag="WJK66_001265"
     CDS             complement(3139..3954)
                     /locus_tag="WJK66_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ7578979.1"
                     /translation="MTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            4198..4983
                     /locus_tag="WJK66_001266"
     CDS             4198..4983
                     /locus_tag="WJK66_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEZ7578980.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLVILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMSGEDGWLESGDIVAANPKVLAQMLKIISAHV"
     gene            5242..7364
                     /locus_tag="WJK66_001267"
                     /pseudo
     CDS             5242..7364
                     /locus_tag="WJK66_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
     gene            7488..9038
                     /locus_tag="WJK66_001268"
     CDS             7488..9038
                     /locus_tag="WJK66_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEZ7578981.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQEAGFL
                     TVESHQYERGWFTSTETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAYIETEFKYAPETEKVLERFFGKQAPVSLANTVYFNGSGKMEVSVSAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKVHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGAFINSEGQFRFGTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTLPSGKIDVGGKIMFK
                     DLKKEDLNQLGLMLKKTEVDIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPDFDEG
                     GMVSEPQQ"
BASE COUNT         2296 a         2170 c         2409 g         2257 t
ORIGIN      
        1 tttgcatttt gaaaatgaga ttgagcataa aattttagta acctatgtta ttgcaaaggt
       61 ctcaaaccgg tttatttacg ttgcaatacg gcggcgaaga agccgtcggt ttggtgtcgg
      121 gcggagttga ggcgcaggta tttgccggta tccaaatcga ttttcaaacc ggcaagcagt
      181 tcggcgcagt tgacgggttc aaattcggga tgttcggaca ggaaacgttc gacttgcagc
      241 tcgttttctt cgggcaggat gctgcaagtg gcgtacacca agcggccttg cggttttacc
      301 agtttggagg cggcatcgag gatgctgtgt tgctgttcca aaaggttggc cacggtttcg
      361 gcggattggc ggtatttgag atcgggatta cggcgtaaag tacccaaacc ggagcagggc
      421 gcatccacta aaacgcggtc ggctttgcct gccagtcggg cgatacggct gtcgtgttcg
      481 ctgctgatgc gttcagggtg gatgttggtc agtccggcgc gggtcatacg cggtttgagg
      541 ttggcaaggc gtttttcggc gatatcgaag gcgtagattc tgcctttgtt cgccatttgc
      601 gcaccgacag ccaaggtttt accgccggca ccggcacaga aatcgacaat gatttcgccg
      661 cgttttgcgc ccaccaataa ggcaagcagc tggctgcctt cgtcttgaac ttccagcgta
      721 ccgtccaaaa acagctcgtg tttgttaagc gcgattttgt ttttcaggcg gatgccccaa
      781 ggcgaataag gcgttgcctc tgcatcggca ctttcggctt gcaacaatgg cagcacttta
      841 tcgcgtttgc ctttcaaggt attgacgcgt atatcgagcg gcgcagcttg gttgatgctg
      901 cggccgaagg cgaggatttc ttcttcgcgc caatgctgct gcagctgttc taccagccat
      961 tgcggcaatt ctgcggcggt attcaggccg tctgaaaact cggttttacg ggctttcaaa
     1021 ttgccgagaa actctatttc ttcttcatca agcaggtctt tgatttggct gatgttggtg
     1081 cttctgccga gaaccagtgc ggcgagtgcg gctttgcgcg gctgcgcgtg cggacggcgc
     1141 agggcggtac tgattttttg atagtggcgc agcgcggcaa aggcggtttc ggcgatttcg
     1201 tggcggtctt ggcggccgag ctttttgtgt tcgcggaaat aggcggagag gacggcatcg
     1261 gaaggctgtt tgaaagtcag catttcagcc aaaactttgg cggtatggtc gagttgtgcg
     1321 gcgttcataa tgtaatgggt tctcttactg tgggttattc ataatcaatc taacgtcctt
     1381 aaaataacaa tagcatcttt atcattactt agtattctat ccgcaataag aaagatgtgt
     1441 taaaagtgcg caatcgggat ttgtctaaat ttctccgtta actacaaagg cagcctgaaa
     1501 aaagttctat tttcaggtcg cttgaacagc atccgatata atcacatcag cgatttcatt
     1561 ttgtaatcac tccctaaaac atgaggaaaa aaacaaaatt tattttcttc gttcagtata
     1621 tctatcctaa atatgccatc actccattga gtttttaatt tgtatttcgc tcttgttaaa
     1681 tgtttttctg tttgccgatt tcttttgcta atgtaaaaaa tactctacca ctctcattgg
     1741 ttaaagtttt caagtattca tcaacatcag taacattaat aacaactgtt tcatttgtat
     1801 gtttattaaa ttctaaatca ataataatat tcccattaac caatatttca tatatatctt
     1861 cttcttcagg gagcatagac aaacaataag cacttgaagc tgaaaactct tttttcagat
     1921 actgaagaag aaaagtattt ttaattaaaa tattattaaa tttatctaat gattgcttga
     1981 cttctttagt tgtccaattt ttaacattat tcttcattgt attctacctg gggtcgtagt
     2041 tattaaatta ccatacttat ttgtttggat cgtcataata ttagttggca tgttgttgtt
     2101 atatttatca tatccaatat ccttattaac tcggatgact ctttcataaa ttgtatggga
     2161 aatgagattt ccatcactaa gcctctcaat agatatacgc gttaatgttg ggaaagggtt
     2221 tcagacggcc ttcataatgc gttcgatttc gtgccaaaag ccgtccggcc gacattccaa
     2281 aacggcaatc aagccttggt cgatacggcg gatttcggtt gcgttcaatg tttcggtttc
     2341 ggcgacacgc gcaggcgcga ggtaggccat acggtcgagc agatggtagc ggacttcttg
     2401 gcgtttaaac ccgtattccg cccaatcttg aatatagatg ccgcgccaac cgtatggatt
     2461 aagcggtggc tcaaaagcat ggaaaccgtg tggaaaaaat ccgatgccgc gcacgatgga
     2521 aacgggtttt acgtcaagcg gcagaccgtg ttgccgcaag gttcggatgc cgtctgaagt
     2581 gtgcggcaga ccgagttggg cggtcagttt ggcggctttt cccaacagcg tgtctttggg
     2641 gtcgagtccg cgcatgtctt cgagcctgcc cgtgccgccg ccgtaatatt tgcaggtcag
     2701 ctcgatatgg tagggcttgc cgttaagcct taccacaaaa tccgccgcgc cttgcgtatt
     2761 gccgtccgaa ccggaaaccg tgaggttgtg cgcgagcagt tcggcgtgtg gcgcgttggc
     2821 aaaccaaaat gccagcagtt cttcggcgta aatgccgagg cggtgtccga acggggcgcg
     2881 tcgggtgagg tgttccgtca gcggcgcggg atcggcatcc aatgccaaaa gatagcggaa
     2941 accgcgttcc cccaatagtt cgcgcacgcc caattcgcaa ccgctttgcc acaaaggcgg
     3001 cgcagtcagc agcgaggcga ggtcgcggac gggttggctg gtgagcttcc accataatgc
     3061 gtctagggcg taattcatgc ttatccgaat atcagtcatg ccgtctgaaa agctggaaat
     3121 gttttcagac ggccttaatc agtctttttt atggatacga tggctgacgg tattgaaaaa
     3181 accgcgcagg atatcgatgt cttcggtttg cgtgttggcg cgtccgaaca ggctctgcat
     3241 acggcgcatc agacgctcgc cgttgcggcg gttgaaaaag ccgatgtcgt tcatcacgct
     3301 ttccatgtgg gcgaccatgc ctttgatttg ctcgtgggtc gcagcgtggt cttcttgttg
     3361 aagatgggtc atgggcgaac cggtttggct gaagatttcg tagcacacga cctgcacggc
     3421 ttgggcgagg ttgagcgaga aatagtcggg attgccgttg atggtcatca gtcggttgca
     3481 ggcttggact tcttcgatgc tcaagccgaa agtctcgttg ccgaaaacca gtgccacttt
     3541 ctcgcctcgg tttgcggtct gcagtaattc ggataccaaa tcgcgcgggg tttgcagcgg
     3601 cgcagtaatt tcgcggcggc ggctggtcag ggcgcaggcg atggtggtgt cggcaagggc
     3661 ttcgtccaaa gaagcggcaa tggtggcatt ttccaaaacg tctgccgcgc cggaagcgag
     3721 gatgaagctt tcttccggta atttaaacga ttgaggatgc tccgggtcaa acacgggcgg
     3781 gttttccgtc atcggcgttg ccatcagatt tggggcgacg atggtcagtt tgtgcagacc
     3841 cattgttttc atcgcgcgcg cggcagagcc gatgttggcg ggatggctgg tgcgcgtgag
     3901 gatgatgcgg atgttgtcca gataagcggg cagggcgggt ttgagagtag tcatgttcgg
     3961 ttttcggtcg gaatggaata gggtataatg cgccgtcttt ttcgttcgga cggtcaaatg
     4021 ccgtctgaaa tgttctttaa caacgtcgga aatttaacgt tgcgcctgaa gcagacacgc
     4081 gtcaggcggt attttacccg atttggcagg atttacccca accaaataaa ctccgatttt
     4141 gccgctccga aggactgtac gtccgaaccg gcggtatgcc acccttaagg aaatctgatg
     4201 aatccgtttt tgaatacagc ctttaaagcc gcccgccgtg ccggtcagat gatgatacgc
     4261 gccgcgggca atctcgatgc cgtcaaaacc gacagcaaag ccttcaacga ttttgtttcc
     4321 gatgttgacc gcaattccga aatcatcctg gttgaggctt tgaaagaagc ctatccgcac
     4381 cacaaaatca cttgtgaaga aagcggctcc cacggcaaag ccgcagccga gtacgaatgg
     4441 attatcgatc cgctcgacgg cacgaccaat ttccttcacg gtcatcccca atacgccatc
     4501 tctatggcgc tgctgcacaa aggcgtgttg caagaagctt tggtttacgc ccccgaacgc
     4561 aacgacgtat atatggcttc gcgcggcaaa ggcgcgttgc tcaacgaccg ccgcatccgc
     4621 gtttccaacc gcatcgaatt gaaccgctgc ctgatcggta ccggcttccc tgttgtcgat
     4681 caaagcatga tggacaagta tctggtgatt ttgaaagatt tcttggccaa aaccgccggc
     4741 ggccgtcgtg aaggcgcggc ttctttggat ttgtgtgctg tagcaacagg tcgttttgac
     4801 ggctttttcg agtttaacct caaaccgtgg gacattgccg caggtgcatt gattgttcag
     4861 gaagcgggcg gtatcgtaac cgatatgtcg ggtgaagacg gctggctgga aagcggcgat
     4921 attgtggctg ccaatcccaa agtgctggcg caaatgttga aaatcatttc cgcacacgtt
     4981 taaatttcag cccgctcccg ggaatgcggg aaaacgggcg ggtatgccgt ctgaagtatc
     5041 gcgccgatat ttcagacggc atttttactg gcagcggggg caggaaggtg gaaacggcta
     5101 aatgcccgaa actgccgagg atggaaacta aacggagaac cctgtgcaat ttgtttatag
     5161 tggattaaca aaaaccagta cggtgttgcc tcgccttagc tcaaagagaa cgattctcta
     5221 aggtgctgaa gcaccaagtg aatgggcaaa gccaacgcct ttaccgcgcc gattaccgtc
     5281 gtcaattacg acgaacaagc cctaaacaac accgaagcgc gtactttggt ggatgccgta
     5341 gcgaaaaaag acgcttccgt ttggcagttc ggcggcgaaa gcaacacgct gaccggactg
     5401 tatttcagag gttatcagct tgactcgcgc caattcagcg tcaacggttt ggcaggtatg
     5461 tacggcacgc aaggcacggc cagcgtgcaa gttggatcgg cacaactgat taaaggcgca
     5521 tccactgccg taaacggcat ggaccctgaa ggtgcggtat ccggttccgt caatatcgaa
     5581 accaaaaaag ccgccgatga aggcaaccgc aaaatcggtt tgggctggtt cagtaacaac
     5641 cgcgcccaag gtacgttcga cttgggccaa cgtttcggcg aaaacaaagc attcggcgtg
     5701 cgcgccaacg gcaaactgcg ccacggcgac accccgcgcc acggttacag cgaagacaac
     5761 aaagaatttg cggtaaatgc cgactatcgc ggcgaaacac tgcgcgtagc gttcgattcc
     5821 atctacgcaa aacgcaaaac caacggcggt cgcgcgcgta tgcaggacat tcaaaacgcc
     5881 ggcggacgct tgtttggcgc gcccgatggt aaaaccaacc tgctgccttc ttggaactgg
     5941 caaaataccg ccggccaaac caatatgctt acgtttgaat gggatgcgtt tgataatgca
     6001 cagattacag gcggtatcgg ctacaacaag gcacgctatt acggcacgct gatttccccg
     6061 accgtatgtt tgaatgctac cagcacttgt accgatatgc aaaacacaaa cgccagcggc
     6121 aaagtaacgg cacgcaacta tgactacaat accggtactg cgcgcctgac cgaccaatat
     6181 ttccgcacat tgagtatgaa cctgaccgca cgcggcgaat ttgaaaccgg tccggtaaca
     6241 cataactgga gcaccgcgtt cgaccgtgtt atccgccagc ggaaaaccat aaacggcagc
     6301 ggaaacggca acagcaagat agaagtgaag gcaaacgaaa atattgcgaa tcaattggca
     6361 tcgttcaaag cctactatcc caattcttgg gcgaaaaccg ccaacttgga tgccaatatc
     6421 aaagttaaca gcctggcttt gtccgacacc ttgggcttcg ttgataacaa ataccgcctg
     6481 actttgggcg gacgtttcca agcggttgaa tacaccgata agaaaaaatc acagagcggc
     6541 gatgccaaac gtttcagccc gatgctgatg gcggcgtggg tgccgcaacc tgatttggtt
     6601 gtgtacggca actatatgga agacttggag cctgccgaca tcaaaaccga tgattccggc
     6661 gaaacgacaa tggcgaaacc gcgtgtcagc cgccagtttg aagtgggcgt gcgtaaaaac
     6721 tggggcaatt tcgtaaccac gttaaacgcg ttccaaatca aacgcccggc tactggcgcg
     6781 gtcatacaac taccaagaca acaaaagggg taacaacaac gctgacctat ggcaacaatt
     6841 ccgatttcgc ccgctataag gcgcaaggcg gcgcggccgg cgacgagcaa ggtatggaac
     6901 gcaatcgcgg tattgagttc aacacctacg cgaacttgct caacaatact ttgcgtccga
     6961 ctttgggtct gatgtatctg caatcgaccg tgaaagagta tccaaattca cgcgatatgc
     7021 tggttaacgg cgtacaagtc gccaatccgc gcgtaattgc caaagcaggt ttggaatggg
     7081 atacaccgtt tgccaaagga ttgactttaa acggtaatgt ttcgtatttc ggcaagtctt
     7141 accaagacac gcaaaaacaa tacgccttcc cgtcctacac cttgattgac gtaggcgcgc
     7201 gctacaaaac caagctcggc aaaaatactt tgaccgtcag cagctcggtg gaaaacctgt
     7261 tcaacaaaaa ctattggcag gtacagcgcg gccaatacga ccgcagcttc gccgtcgtcg
     7321 gtatgccgcg cacttactgg ctgaaggcgg aattggattt ctgatttccc tatatccgga
     7381 aaatgccgtc cgaacaggtt tcagacggca tttcttttaa agagatatgg cgggatacgc
     7441 ttaaataatg ctattctagc gtctttgaaa cctgcttgtt tgaagtgatg aaaaaacctt
     7501 tgatttcggt tgcggcagca ttgctcggcg ttgctttggg cacgccttat tatttgggtg
     7561 tcaaagccga agaaagcttg acgcagcagc aaaaaatatt gcaggaagcg ggcttcttga
     7621 ccgtcgaatc gcaccaatat gagcgcggct ggtttacctc tacggaaacg acggtcatcc
     7681 gcttgaaacc ggagttgctg aataatgccc ggaaatacct gccggacaac ctgaaaacag
     7741 tgttggaaca gccggttacg ctggtaaacc atatcacgca cggccctttc gccggcggat
     7801 tcggcacgca ggcgtacatt gaaaccgagt tcaaatacgc gcctgaaacg gaaaaagttt
     7861 tggaacgctt ttttgggaaa caagccccgg tttcccttgc caataccgtt tatttcaacg
     7921 gcagcggcaa gatggaagtc agtgtttccg ccttcgatta tgaagaactg tcgggcatca
     7981 ggctgcattg ggaaggtctg acggggaaaa cggtttacca aaaaggtttc aaaagctacc
     8041 gcaacagcta tgatgccccc ttgtttaaaa tcaagctggc agacaaaggc gatgccgcgt
     8101 ttgaaaaagt gcatttcgat tcggaaactt cagacggcat caatccgctt gctttgggca
     8161 gcagcaatct gaccttggaa aaattctccc tagaatggaa agagggtgtc gattacaacg
     8221 tcaagttaaa cgaactggtc aatcttgtta ccgatttgca gattggcgcg tttatcaatc
     8281 ccaacggcag catcgcacct tccaaaatcg aagtcggcaa actggctttt tcaaccaaga
     8341 ccggggaatc aggcgcgttt atcaacagtg aagggcagtt ccgttttggc acactggttt
     8401 acggcgatga aaaatacggc ccgctggata ttcatatcgc tgccgaacac ctcgatgctt
     8461 ctgccttaac cgtattgaaa cgcaagtttg cacaaatttc tgccaaaaaa atgactgagg
     8521 aacaaatccg caatgatttg attgccgccg tcaaaggcga ggcttctgga ctgttcacca
     8581 acaatcccgt attggacatt aaaacttttc gtttcacgct gccgtcagga aaaatcgatg
     8641 tgggcggaaa aatcatgttt aaagatttga agaaggaaga tttgaaccaa ttgggtttga
     8701 tgctgaagaa aaccgaggta gacatcagaa tgagtattcc tcaaaaaatg ttggaagact
     8761 tggcggtcag tcaggcaggc aatattttca gcgtcaatgc cgaagatgag gcggaaggca
     8821 gggcaagtct tgacgacatc aacgagacct tgcgcctgat ggtggacagt acggttcaga
     8881 gtatggcaag ggaaaaatat ctgactttga acggcgacca gattgatact gccatttctc
     8941 tgaaaaacaa tcagttgaaa ttgaacggta aaacgttgca aaacgaaccg gagccggatt
     9001 ttgatgaagg cggtatggtt tcagagccgc agcagtaatg ggtcaaatgc cgtctgaaat
     9061 gttcaggcgg cattttgttg tattggaaac tgccgttttc aaccattcct ttagataagc
     9121 cgtgtcggat tc
//
LOCUS       DAWKOG010000074         8963 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.074, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000074 DAWKOG010000000
VERSION     DAWKOG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8963
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.074"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(220..2910)
                     /gene="glnE"
                     /locus_tag="WJK66_001269"
     CDS             complement(220..2910)
                     /gene="glnE"
                     /locus_tag="WJK66_001269"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEZ7578982.1"
                     /translation="MSDNRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKVWTEADFQ
                     AFADWDKIRAEENEEELARQLRGLRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFSYAYYQDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFIYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNDIKSLVRPFVFRKYLDYGAYEAMRKLHRQIHS
                     EVGKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTRETLEKLAESGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDRQTQTLPASPEQRQLLAESMGFDDYADFLNAL
                     NQHRNQVNRLFNEILSEPEEQTQSNSEWQWVWQDKPDEEERQGRLKEHGFDAETIATR
                     LDQIRHSHKYRHLSAHAQPRFDAVVPLFVQAAAAQSNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAQIMGQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DDLKACGGDTEAQMDTLRHFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTILAAAL
                     LCAWADMPKKHRDTPQFAVVGYGKLGGKELGYASDLDLVYLYDDPHPEAGDVYSRLAR
                     RLTNWLSTATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEILTAERDQTALADEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARKYPQLLDNYGNIALLNIATDCGLIDKTLAEQSRTAYRFYRRQ
                     QHNTKLRDAEKTEVSGELLAHYGNVRKLWREVFGEEAATA"
     gene            complement(3128..3310)
                     /locus_tag="WJK66_001270"
     CDS             complement(3128..3310)
                     /locus_tag="WJK66_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578983.1"
                     /translation="MGFWNGVAKAVGEGMIEAGNEHKALKMEYAEKSSEELHEIVKSD
                     GFLKIPHGRKVRLMLF"
     gene            complement(3447..3920)
                     /locus_tag="WJK66_001271"
     CDS             complement(3447..3920)
                     /locus_tag="WJK66_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HEZ7578984.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            complement(3973..4254)
                     /locus_tag="WJK66_001272"
     CDS             complement(3973..4254)
                     /locus_tag="WJK66_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578985.1"
                     /translation="MENGQRNNRFPLEKRIFYLEHSGRYLMICALSDYSQNKHTVVMA
                     NFIYPDEKTDWRNLDDLFNELVLEELQASFMDWHPTVEEAISRHLEDFS"
     gene            complement(4341..4664)
                     /locus_tag="WJK66_001273"
     CDS             complement(4341..4664)
                     /locus_tag="WJK66_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7578986.1"
                     /translation="MFKDELNEFIRLISDPESELDEWYLSDFKDEHIWEMQSYEAFSC
                     LREAVPYLFAYPRYGYELLEIISALKETSDTTELFYEPGIVPLLIDLYKEDSYLVNMV
                     KRIFK"
     gene            complement(4997..6004)
                     /locus_tag="WJK66_001274"
     CDS             complement(4997..6004)
                     /locus_tag="WJK66_001274"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEZ7578987.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALYTVYKLGLIPVTGNRTKPV
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPKPQPGNPQPRLFRVPE
                     HQGIINRMGFNNHGIDTMIRNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVYGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGLPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGKDAAEKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            6236..6472
                     /gene="acpP"
                     /locus_tag="WJK66_001275"
     CDS             6236..6472
                     /gene="acpP"
                     /locus_tag="WJK66_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ7578988.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            6628..7875
                     /gene="fabF"
                     /locus_tag="WJK66_001276"
     CDS             6628..7875
                     /gene="fabF"
                     /locus_tag="WJK66_001276"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEZ7578989.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGNSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            8230..>8963
                     /locus_tag="WJK66_001277"
     CDS             8230..>8963
                     /locus_tag="WJK66_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ7578990.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKELKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYHIALPMLESLIVLNPDD
                     KDELTDKYGIKIKNIHILGGIGLDLRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKDKYPDTVFTALGAIDKSR"
BASE COUNT         2142 a         2374 c         2247 g         2200 t
ORIGIN      
        1 tcttatttgt ttgttttgat ttttgcttgg ataaaaaatc ccatcattcc cacaaaaaca
       61 gaagcctgaa atcccgtcat tcccgcgaaa gaggaatccg gtttttgggt ttcagccatt
      121 cccgataaat cgctttagct ctgccgcaac cttaaaacgg cacggtacga aaataccgtc
      181 tgaaacccag attgtcaggc ttcagacggc attttttatt caggcggttg ccgcttcttc
      241 gccgaacact tcccgccaca atttcctgac attgccgtaa tgtgccagca attcgccgct
      301 tacttcggtt ttttctgcgt cgcgcagttt ggtattgtgt tgctgccggc ggtagaagcg
      361 gtaggcggtg cggctttgtt ctgcaagggt tttgtcgatg aggccacagt cggtggcgat
      421 gttcaagagg gcgatgttgc cgtagttgtc caagagttgc ggatattttc gggcgtgggc
      481 aagtatcaga tattggacga taaattcgac atcaaccacg ccgccgcgtg cgtatttgac
      541 gttgctgtcg gctgggggat gagtggggaa cattttttcg cgcatttcga tgatttcgtc
      601 tgccaaggcg gtttggtcgc gttcggcggt aaggatttcg gtgcggatgc ggtcgaatgc
      661 cgtctgaatt tcgggtgtgc cgcagatgaa gcgggcgcgg gtaagggact ggtgttccca
      721 cgtccacgcg ttttcgcgct ggtatttttc aaaggcggcg atgctgtgcg cgaggaaacc
      781 tgcgtcgccg ttggggcgca ggcgcaggtc ggtttcgtag aggctgcctg cgccggtggc
      841 ggtggaaagc cagttggtca ggcggcgggc gaggcggctg tacacgtcgc ctgcttcggg
      901 atggggatcg tcgtaaagat agaccaagtc gaggtcggag gcgtagccga gttctttgcc
      961 gccgagcttg ccgtagccga cgacggcaaa ttgcggggtg tcgcggtgtt ttttgggcat
     1021 gtccgcccac gcgcacagca gggcggcggc gaggatggtg tcggcaaggg cggagagctg
     1081 gtcggagagg gattctaccg tccacagtcc ggcgaggtct tgaacggcga ggcggaagac
     1141 ttgggcgtgt tggaaatggc gcagggtgtc catttgcgct tcagtatcgc cgccgcaggc
     1201 tttgaggtcg tctgaaaggg cggcggcgag cgcctgccaa tcaaacgcgg tatccaaaag
     1261 ctgcgcgctg atgagttcgt ccaacaaaat cggatatttg ttcagatacg ccgccaccca
     1321 agaactttgg cccataatct gcgccagttg cgccaaggtt tgcggatgtt cgttgaggaa
     1381 ggcgagatag gcggatcggc ggctgatgtt ttcgagaaaa tccaacagcc gcatcaatgt
     1441 atcggtcggg ttgctttgcg ctgccgccgc ctgtacgaac agcggcacaa ccgcatcgaa
     1501 acgcggctgg gcgtgtgcgg agaggtggcg gtatttatgg ctgtggcgga tttggtcgag
     1561 ccgcgtggca atggtttcgg catcaaaccc gtgttctttc agacggcctt gccgttcttc
     1621 ttcgtcgggc ttgtcctgcc aaacccattg ccattcgctg ttgctttgcg tttgttcttc
     1681 gggttcgctc agaatttcgt tgaacaaccg attgacttgg ttccgatgtt gatttaaagc
     1741 attcaaaaaa tcggcgtaat catcaaatcc catactttcg gcaagcagtt gccgctgttc
     1801 gggcgaagcc ggcagggttt gggtttgccg gtcgtcccaa tattgcaaac gatgttcgac
     1861 atcgcgcaaa aagcggtagg cggcaagcag ggtttcgacg ttttcagacg gcataatgcc
     1921 cgattcggcg agtttttcca gcgtttcccg cgtgcctttc agttgcagcg cgcgcatttg
     1981 cccgccgcgt atcatctgga aaatctgggc gataaattcg acttcgcgga tgccgcccgc
     2041 gccgagtttg atgttgtccg ccatcccttt tttgccgact tcgctgtgga tttggcggtg
     2101 cagcttacgc atcgcctcat acgcgccgta atccagatat ttgcggaaca cgaaggggcg
     2161 caccagcgat ttgatgtcgt tcggatacgg cgtgaccacg cggcctttgc accacgcgta
     2221 gcgttcccat tcgcgcccct gcgtaatcaa atattgctcc agcgcggttt cgctcaatac
     2281 cagcgcgccc gaatcgccgt ccggccgcag ccgcatatcg acgcggaaca cctgcccgtc
     2341 ggcggtaatg tcattcagca acgcaatcag tttctgcccg actttggtga aaaattcctg
     2401 attgccgcgt tcgcgcctac cgtcggtatc gcccgattcg gggtagataa aaattaaatc
     2461 gatgtcggaa gacacgttca actcatagcc gcccgccttg cccatcgcca ccacgctcaa
     2521 atgctgcggc gatttggtat aacgcccgat cggcgtgccg tacatatcct gataataggc
     2581 gtatgaaaaa tccaatgcgg tattcacagc aaaatcggca aacagcgtaa tcgtgcgggt
     2641 tacttcgttc aaatcgctga tgcggttgat atcgcgcacg ataatctgcg acaccacata
     2701 acggcgcaac ccgcgcaact gccgcgccaa ttcttcctcg ttttcttccg cacggatttt
     2761 gtcccagtcg gcaaaggctt ggaaatccgc ttcggtccaa accttgtcca gcataggcag
     2821 gaaaatttcg ggcttgagtt tgccgttgtc gagctggcgg gcgaggaaga gggaatggcg
     2881 gcgggcggtg tcgaggcggt tgtcggacat ttcggattcc gtttgaaaaa atggcaggaa
     2941 gatgatggcg gattcacttc aaatcaggac agggcgaggg gatgccgtac cggtttaaag
     3001 tcaagccact atatcatagc aaccttcgat gccgtctgaa accttgcccc gacatccaga
     3061 cggatgcctg ccgataaaaa acagcggcaa atgccgccgt ttcctgttca cacctcgcca
     3121 cgctctttta aaatagcata agccgcactt ttctcccgtg tggaattttt aaaaaaccat
     3181 cactcttgac gatttcatgc agctcctcac ttgatttctc cgcatattcc attttcaacg
     3241 ccttatgctc attgccggct tcaatcattc cctctcccac tgcttttgcc acaccattcc
     3301 aaaaacccat aatcatgtcc tccaatcgtt tcaaataaat aaggtcgttg tttctgtcaa
     3361 gaaacaacga cagtttataa actgtcggaa aacaaaatgc cgtctgaagg ccggataatg
     3421 cttcagacgg catcggattg ccggttttaa ccctgccctc cgccgttgtt caaatagtgt
     3481 tcccagtgag aatataaccg ggcaatattt tcttcatagg tgtgggcaga aggaatataa
     3541 ccgtctttgg gcggcatata cctgacaaat gattctattt cttccggata atcaaaatct
     3601 gcataaattt tttcaacttc ccccaaagga tcctcaaatt cttcttttct atggaataca
     3661 tcgcttaaga ggacgtacag ccaattttta ggggaataat ccctcatttc cgaagccaaa
     3721 tcttccagaa caggctttaa ttcaagaata ttggatttgt tgattaaaga tagtttgaac
     3781 acacgttcat catgatctga aagcgtcatt tttcgggcat aagctgccac atcagcccaa
     3841 cccaagtatt tattcccata cccccacaga atatccttcc aagaaagacg gactttttgc
     3901 gcggttaaat caaggttcat tttttactcc gattgcctgc cccgtcattc cgccccttcc
     3961 gattgacgcc tgttacgaaa agtcttccaa atgacggctg attgcctctt caacagtcgg
     4021 atgccaatcc ataaatgaag cctgcaattc ctctaaaacc aactcattga ataaatcatc
     4081 caaattccgc caatccgttt tttcatccgg atagatgaaa tttgccataa cgacagtatg
     4141 tttgttttgg gaataatccg ataaggcgca aatcatcaga taccgccccg aatgctccag
     4201 ataaaaaatt cgtttttcca aaggaaaccg gttattccgt tgcccgtttt ccatttttca
     4261 tcctttttaa cgtatcggct tccgcgtcct tcaaattgag ttacctgcct tccgccaagc
     4321 aaacgcccgc gcccctttca tcatttgaat atccgtttga ccatatttac cagataacta
     4381 tcttctttat ataaatcaat cagcaaaggc acgatacccg gctcataaaa taattctgtc
     4441 gtatcagatg tttcttttaa cgcggaaatt atctccaaca gttcataccc gtatcgggga
     4501 taagcaaaca aataggggac tgcttcccgc aaacatgaaa atgcttcgta actttgcatt
     4561 tcccatatat gttcatcttt aaaatcactc aaataccatt catccaattc actttccgga
     4621 tcggatatca atcggataaa ttcatttagt tcgtccttaa acatatcaaa tccttaaata
     4681 taaaatcttt tgattatccg ccgtatcctt attttccaca tcatgaattt caacgccgtt
     4741 catattttgc gttttcccaa gccgtccgcc acagtataaa attatttttt gtatcaattg
     4801 cttatatatt taacaactta ccgccggact tgctttggct gcgtatcaag attgtccggt
     4861 tcgcggcggg cgtttgcttt gtttcaaaca gggcggcgcg taagggattt tcaaggggcg
     4921 tcaggcggaa aaaggaaatg aaaatgccgt ctgaacggca aaacgtgcgt tcagacggca
     4981 ttttgggcgg atcgcgtcat cgcgccaaag ccttcaaaca ttctttgacc aatgccggac
     5041 ctttgtatat caatccgctg tacacctgga cggcagttgc gcccaagcgg attttctctg
     5101 ccgcatcctt gccttccata atgccgccta cgccgataat cggcagcttg ccgtctatgt
     5161 ggtctgccaa cagcttcaac acccgattac ttttttcatg aacgggcagc ccgctcaaac
     5221 cgccctgctc gcctgcgagc ggatggctgc cgagacttga tttgtcgatg gtggtattgg
     5281 tagcgatgat gccgtccatt tcgacggatt tgacaacgtg ggcgatgtct tcgatttgtg
     5341 cttcatccaa atcgggggcg attttgacgg cgagcggaac gtatttcccg tatacagagg
     5401 caagctgtgc ctgtttgttt ttcaaagcct caagcaatgc gctcaactcg tcgccacctt
     5461 gcagcgcgcg gaggtttttg gtgttgggcg aggaaatgtt gacggtaatg tagcttgcgt
     5521 gcgcgtaggc tttttcaagg cagattaaat aatcatcggc agcgttttcg atgggtgtaa
     5581 ccgcgttttt accgatgttg atgcccaata cgccactgaa tttacttttt tcgatgttgc
     5641 gtatcatggt gtcgataccg tggttgttga aacccatgcg gttgatgatg ccttggtgtt
     5701 cgggaacgcg gaagaggcgc ggctgcgggt tgccgggctg cggtttgggc gttaccgtgc
     5761 cgatttcgat gaaaccaaag ccgagcgcgc ccaatgcgtc gatgtattcg ccgtttttgt
     5821 cgagtccagc ggcaagtccg acagggttgg gcaaatccat acccatcaat tttacaggtt
     5881 tggtacggtt gccggttacg ggaatcagac ccaatttata aaccgtgtag agcgcgtcga
     5941 gcgtgaagtg gtgggctttt tcggcatcga gtgcaaacag gatgcggcgg gcaagtggat
     6001 acatgacagg gctttctgct tcagatggaa atgccgccat tttaaccgaa atcccgccaa
     6061 acatacggca cacaggtcaa acttgaacga caaaacccgc cgcacccaaa aatgtacaaa
     6121 tcggcaaata ttgatataat agtcattaga tagaaaactc tattaaaagt cggtacaata
     6181 ccaccgattt atttccctta aacaacctgc cgcaaggcat aaaggaacga ctgatatgtc
     6241 aaacatcgaa caacaagtta aaaaaattgt tgctgaacaa ctgggcgtaa acgaagccga
     6301 cgtgaaaaac gaatcttctt tccaagacga cttgggcgcg gattctttgg acaccgtgga
     6361 gctggttatg gctttggaag aagcattcgg ctgcgaaatc cccgacgaag atgccgaaaa
     6421 aatcaccacc gtccaactgg ctatcgacta catcaatgcc cacaacggct aaccggtcgt
     6481 cgcccgacac aacagcctct gctgcaccgc gcaatagagg cttttccctt atatggcaaa
     6541 ctgtttgaac ccctgccgca aattacagac ggcatctgca cgacagccgc atatgccccc
     6601 attccgacaa ccaacagcga ggttatcatg agtcagagaa gagtagtcat tacaggctta
     6661 ggtcaggttt cccctgtcgg caacactgtc gcagaggctt gggacaccct gctcgccggc
     6721 aaaagcggca tcggcgcgat tacccgcttt gacgcatccg acatcaacag ccgtgtcgcc
     6781 ggcgaagtgc gcggtttcga catcggacaa tacatcagcg cgaaagaagc gcgccggatg
     6841 gacgtattca tccactacgg cattgccgcc gcattgcagg caatcgccga ttcgggcttg
     6901 gacgatgtgg aaaacctcga caaagaccgc atcggcgtga acatcggttc cggcatcggc
     6961 ggactgccca gcatcgaggt caccggcaaa gccgtaatcg aaggcggcgc gcgcaaaatc
     7021 aaccctttct ttatccccgg ttcgctgatc aacctgattt ccggacacgt taccatcctc
     7081 aaaggctacc gcggcccgag ctacgggatg gtttccgcct gtaccaccgg cgcgcacgcc
     7141 atcggcaatt ccgcccgact gatcaaatac ggcgacgcgg acataatggt tgccggcggc
     7201 gcggaaggcg caatcagcac tttgggcgtg ggcggttttg ccgcgatgaa agccctctcc
     7261 acccgcaacg acgaccccgc caccgcttcc cgtccgtggg acaaaggccg cgacggcttc
     7321 gttatcggcg aaggcgcggg catattggtg ttggaagaat tggaacacgc caaaaaacgc
     7381 ggcgcgaaaa tctacgccga aatcgtcggc ttcggcatga gttccgatgc ttaccatatc
     7441 accgcgccga acgaagaagg ccccgccctt gccgttaccc gcgcgctgaa agatgccggc
     7501 atcaatcccg aagacgtgga ttacgtcaac gcgcacggca cgtccacccc cttgggcgat
     7561 gccaacgaaa ccaaagccct caaacgcgcg ttcggcgaac acgcctacaa aaccgtcgtc
     7621 agctcgacca aatccatgac cggccacctg ctcggcgcgg cgggcggcgt ggaggccgtg
     7681 tacagcattt tggcgataca cgacggcaaa atcccgccga ccatcaacat ttttgaacaa
     7741 gacgttgaag ccggctgcga tttggactac tgcgccaacg aagcgcgcga cgcggaaatc
     7801 gacgttgcca tttccaactc cttcggcttc ggcggcacca acggcacgct ggtcttcaaa
     7861 cgcttcaaag gctgattccg caaagtcgcc gccgacatcg aaatgccgtc tgaaaccgtt
     7921 tcagacggca ttttcaaaac accccgcccg ttaccggcac ttcgcggcac acaaggcagc
     7981 cttataatac gcaggcaaac ccaagccctg ccggattgcg ggacgaaacc ctgccgtagc
     8041 agaccgtatt tccaatcata tattcaaggt gtccgcctta tgtttcaaca cacaggacga
     8101 cacataaagc accgccctat gtgtcgtcct gatttggaag gggttacgcc ccctcccaaa
     8161 tagagtctga ttctaccgcc ccgaaggaca gatgtccgag tggcggggtt caaccgaaaa
     8221 ggaaacacga tgaaaatcgt ttttatcaca acagtcgcat ccagcattta cggtttccgc
     8281 gcccccgtca ttaaaaaatt aatcggcaaa aaccatcagg tgtatgcctt tgtatcggag
     8341 ttttccgata atgaattgga tattatcagg gaaatggggg ttacacccgt tacctaccgt
     8401 tcaaaccgca gcgggctgaa cccgttttcc gatataaaat ccaccttcct catctttaaa
     8461 gaactcaaaa aaatatcgcc ggatttggtt ttcccttatt tcgcaaaacc cgtgattttc
     8521 ggcacttttg ccgcaaaact ggcaggcgtg cccagaatcg tcgggatgct ggaaggtttg
     8581 ggattcgcat ttaccccgca gccggaaggc ataccgttaa aaacaaaaat cataaagggg
     8641 attttgattg ccttgtacca cattgccctg ccgatgttgg aaagcctgat tgtattaaac
     8701 cccgacgaca aagacgaact gacggacaaa tacggcatca aaataaaaaa catccatatt
     8761 ttgggcggaa tcggtctgga tttgcggcaa tatccttatt ccgaggcgga tattcccgat
     8821 gaaaaagaac ccgtaaaatt cctctttatc ggcagatttc tgaaagaaaa ggggattgat
     8881 gattttattc gggcggcgga acaggttaag gacaaatacc ccgatacggt ttttaccgct
     8941 ttgggcgcaa tcgacaaatc acg
//
LOCUS       DAWKOG010000075         8911 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.075, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000075 DAWKOG010000000
VERSION     DAWKOG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8911
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.075"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            136..2016
                     /locus_tag="WJK66_001278"
     CDS             136..2016
                     /locus_tag="WJK66_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HEZ7578991.1"
                     /translation="MNIFYEESGQFKIAAIIQKNDATYQVDTPHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLPLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLGNGIKRVGIHIAAPS
                     LAIKQGDKMEKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGTYRPSISIYFDVDNE
                     FNVGAPTCKIEAVNIAENLRIQAIEPHFNAEAGLDEAGEMMFAHHQDLIWFYQFATAL
                     QKARGKYEPDRAPQYDYSIELDKEDNVSVVRRERGSPIDTLVSEMMILANSTWAQMLH
                     DNDLPGLFRVQPTGKVRMSTKSEPHIGMGVRHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGTSELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVAPAGTTAGN
                     AV"
     gene            complement(3250..4275)
                     /locus_tag="WJK66_001279"
     CDS             complement(3250..4275)
                     /locus_tag="WJK66_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ7578992.1"
                     /translation="MKIKALMIAAALLAAADVHAAPQKAKTASAKAAKAAKAAKVAKV
                     AATAQKEAAPAQQQGGIRFSDGIAAVADNEVITRRRLAEAVAEAKANLPKDAQISESE
                     LSRQVLMQLVNQSLIVQAGKRRNIQASEAEIDAVVAKNSALKNLSPAQRRDFADNIIA
                     EKVRQQAVMQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQHILIKADSENAAV
                     GAESTIRKIYGEARSGTDFSSLARQYSQDASAGNGGDLGWFADGVMVPAFEEAVRSLK
                     PGQVSAPVRTQFGWHIIKLNEVREAGTPQERIRNSVRQYIFQQKAEQATVNLLRDLHS
                     GAYVDIR"
     gene            complement(4380..6788)
                     /locus_tag="WJK66_001280"
     CDS             complement(4380..6788)
                     /locus_tag="WJK66_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEZ7578993.1"
                     /translation="MARLFSLKPLVLALGFCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVQGSGEASIPEDYTRIVADRMEGQSQ
                     VQVRAEGNVVVERNRTTLNADWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMETEHGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPIFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPGVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GMTFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKNDAVMLDGSVGKKPRSRS
                     DWVAFASSGIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
     gene            6858..7862
                     /locus_tag="WJK66_001281"
     CDS             6858..7862
                     /locus_tag="WJK66_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEZ7578994.1"
                     /translation="MQRQTELKNWLQTIYPERDFDLSFAAADADFRRYFRATFSDGGS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQILHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGETAHKALLLEAIGELVELQKASRKGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARATGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            complement(7919..8617)
                     /locus_tag="WJK66_001282"
     CDS             complement(7919..8617)
                     /locus_tag="WJK66_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ7578995.1"
                     /translation="MKSKHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLTLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAH"
BASE COUNT         1858 a         2413 c         2521 g         2119 t
ORIGIN      
        1 gtactggttt ttgttaatcc actataacgc aagcaccgca aagacgcgcc aaccctctcc
       61 caaccttttt tcagacggca ttttcggtaa tctgctaaaa tcgcccgctt gagtttccac
      121 agaaaaatcc gaaaaatgaa tattttttac gaagagtccg gccaattcaa aatcgccgcc
      181 atcatccaaa aaaacgatgc cacctaccaa gtcgataccc cacacggcaa acgcaccaaa
      241 gtgaaggcga acaacgtctt tgccgagttt gacggcgata tggcggcgtt tttggaaaac
      301 gcgcaggcac aggcggcaga catcgacacc gatttattgt gggaagtatg cggcgaagag
      361 gaatttaccg ccgaagccat cgccgaagaa tattacggcc atgcgccgac caaaaccgag
      421 ctggcggcaa ctttgattgc gctttacgcc gcgccgatgt atttctacaa aaaagccaaa
      481 ggcgtgttca aagccgcgcc cgaagaaact ttaaaacaag cacttgccgc catcgaacgc
      541 aaaaaacagc aagacgcgca aatcgacgct tgggcggaag ccttgaaacg cggcgagatg
      601 ccgtctgaaa tcgcggcgga tttgaaaacc atcctgcacg cgcccgacaa gcagtcgctg
      661 acctacaaag cctttaccaa agccgccgac gcgctgaaaa cctctgccta cgaattggcg
      721 aaaaaaacgg gcggcattac gtccattccc caatacctgc aagacgggtt tgaaatcaaa
      781 tatttcccca aaggaacagg cttccccgac cttccgcttc ctgaaatgcc tgacttaccc
      841 aaagccgacg ttaccgcctt ttccattgac gacgaatcga ctaccgaagt ggacgacgct
      901 ttaagcctga ccgatttggg caatggcata aaacgcgtcg gcatccacat tgccgcgccg
      961 tcgcttgcca ttaagcaagg cgacaaaatg gaaaaaaaca tcatggaacg cttaagcacg
     1021 gtttattttc ccggcggcaa aatcacgatg ttgcccgaaa actggattgc cgcgttcagc
     1081 cttgatgcag gtacataccg cccttccatc agcatttatt tcgatgtgga caacgagttc
     1141 aacgtcggcg cgccgacctg caaaatcgaa gcggtcaaca tcgctgaaaa cctgcgtatc
     1201 caagccatcg aaccgcattt caacgccgaa gcgggcttgg acgaagcggg cgaaatgatg
     1261 ttcgcccacc atcaagacct gatttggttt tatcaattcg ccaccgccct gcaaaaagcg
     1321 cgcggcaaat acgaacccga ccgcgcgccg caatacgatt acagcatcga attggataaa
     1381 gaagacaacg tatccgtcgt ccgccgcgaa cgcggttcgc ccatcgatac gctggtcagc
     1441 gagatgatga ttcttgccaa cagcacttgg gcgcaaatgc tccatgacaa cgacctgccc
     1501 ggcctcttcc gcgtccaacc caccggaaaa gtacgcatga gcaccaaatc cgagccgcat
     1561 atcggcatgg gcgtgcggca ttacggctgg tttacctcgc cgctgcgccg cgccgccgac
     1621 tacatcaacc aaaagcagct gatcagcctg atagacgaca ctgccgagcc gctgtatcaa
     1681 aacagcgatg ccgagctttt cgccgcactg cgcgactttg atgccgccta taccgcctac
     1741 gccgacttcc aacgccaaat ggaagcctac tggagcctgg tttacctgca acagcaaggc
     1801 acgagcgagc tgacggcgac gattctgaaa gaagacctcg tccgcatcga aggtctgccg
     1861 ctagtaacac gcgcgaccgg tattccgttt gacgcgctgc ccaaatcgca ggcattgttc
     1921 aaaatcaccg aattagatgc cgagaagcag tttgtctcgc tcaactacat caaggcagtc
     1981 gcacccgcgg gtacaacggc aggcaatgcc gtctgaagcc cgatacggca aactccggca
     2041 aaaacagaaa cctgaatctc atcattccca cagtcgggaa tccggttttt tgggtttccg
     2101 ttgtttttcc gtttcaatga acttccaagc cgccgttccc gataaatacc cgcaatctaa
     2161 aatcccgtca ttcccacgca gatgggaatc taggtttatc cgcacagaaa ctgatatgcc
     2221 gtcattcccg cgaaagcgga aatccagatt cttcggtaca gaaacttatc ggataaaacg
     2281 gtttctttag attctacgtc ctagattccc gcctgcgcgg gaatgacggc ataggggttt
     2341 ccgttttccc gataaattac cacaacccaa aatcccgtca ttcccgtaaa agcgggaatc
     2401 cggttcgttc ggtttcgttt tttttgagtt tcgggcaact tctgaatcgt cattcccact
     2461 caggtgggaa tctaggtctg tccgcacggg aacttatgcg ccgtcattcc cgcgaaagcg
     2521 ggaatccaga ccgttcggct tcagtttttt ggtttcgggc aacttctgca tcgtcattcc
     2581 cacacaggtg ggaatctagg tctgtccgca cgggaactta tgcgccgtca ttcccgcgaa
     2641 agcgggaatc cggttcgttc ggtttcgttt tttttgagtt tcgggcaact tctgaatcgt
     2701 cattcccaca caggtgggaa tctaggtctg tccgcacggg aacttatgcg ccgtcattcc
     2761 cgcgaaagcg ggaatccaga cccttcggca cagaaactta tcggataaaa cggttgcttt
     2821 agattctacg tcctagattc ccgctttcgc gggaatgacg gcatagaggt ttctgttttc
     2881 ccgataaatt cctgttactt ttcgttgcta gattcccgcc tacgcgggaa tgacgatgga
     2941 aagattgttg ttgcttcgga taaatttctg cagttttaaa taaccggatt cccgcctgct
     3001 cgggaatgac ggcataggtt tttttgtttt tccgataaat taccacaacc caaaatcccg
     3061 tcattcccgc gacggcggga atccagtcct ttaaactcca gccattcccg ataaattcct
     3121 gttacttttc gtttctagat tcccgctttc gcgggaatga tgaatgacgg tgtaaaagta
     3181 actcgaaatc caaaaagcca taccgcccgg atttttgccg atcggtatgg ctttttgctt
     3241 caaaccgcct tagcggatgt cgacatacgc gccggaatgc aggtcacgca acaggttgac
     3301 ggttgcctgt tcggcttttt gttggaagat gtattgccgc acggaattgc ggatacgttc
     3361 ctgaggtgtg ccggcctcgc gtacttcgtt cagcttgatg atgtgccagc cgaactgggt
     3421 gcgtacgggc gcgctgactt ggccgggttt gaggctgcgg acggcttctt caaaggcggg
     3481 aaccattacg ccgtcggcaa accagcccaa gtcgccgccg ttgcccgcgc tcgcgtcttg
     3541 cgaatattgg cgcgctaggc tggaaaagtc tgtgccgctg cgggcctctc cgtagatttt
     3601 gcggatggtg ctttccgcgc cgacggcggc gttttcgctg tcggctttaa tcaggatgtg
     3661 ttgggcgcgg tattggcgca acggtgcgcc ttcgggcagg gtgatgcctt gtttttgcgc
     3721 ctgctcgagg aaggcatcga tttcagcttc gctcacgcgg ctgttctgca tcactgcctg
     3781 ctggcggact ttttcggcaa tgatgttgtc ggcaaaatcg cggcgttggg cggggctgag
     3841 gtttttgagg gcggaatttt ttgcgacgac ggcatcgatt tccgcttcgc ttgcttgaat
     3901 gttgcggcgt ttgcccgcct gtacaatcag ggattggttg acaagctgca tcagcacctg
     3961 tcgggacagc tcggattcgc ttatctgcgc gtctttgggc aggttggctt tggcttcggc
     4021 aacggcttcg gcaagccggc ggcgcgtgat gacttcgttg tcggcaacgg cggcaatgcc
     4081 gtctgaaaag cggataccgc cctgctgttg tgcgggtgcg gcttcttttt gcgccgtggc
     4141 ggcaactttg gcaactttgg cagctttggc agctttggca gctttggcgg atgcggtttt
     4201 tgccttttgc ggtgcggcgt ggacatcggc tgctgccagc aatgcggcgg caatcatcag
     4261 ggctttgatt ttcatcattc tttccttatc gtttgaatcc tgcctttgat ggcgggtttg
     4321 gggttgtcgg tggaatgccg tcttgtgcgg gaacggtgct cctgccgggt ttccgacggt
     4381 cagggccgtt tgttgcgtcc ggcggaaaga gagtgggcgg ggatatagcc gggaacggcg
     4441 acatccatcc tgtctgcggg gtttctgccg acactgctga ggtctttcaa ctgaagtgag
     4501 aaaaagacag cgtttttgta ggtgttttcg ccggtaacgt agcgttgggc gtacacgccc
     4561 gcgccccagc agccgcaact gcttttgtat tccgcacccg ccagcacctc tatcggtttt
     4621 ttggcttcaa aaccgtagtt gtaacggacg acggccgaca ggttgcgcgt cagcggccat
     4681 tgtgcggaca ggtcgagctg gctgagtttg tcgtaaaaat aggaaccgtc ggacttcagg
     4741 tagatttttt cgttgcgccc gtatttgtag cgggcgttca gcactttgcc ctgtgcggga
     4801 cggtagcttg caccgacggc gtagttctcg gcgcgtttgt cgttttggtt gtagtggatg
     4861 ctgctgtcga ggatgaagcg gctgccgatg ccgctggagg cgaatgccac ccagtcggaa
     4921 cggctgcgcg gttttttgcc gacactgccg tcaagcatga ctgcgtcgtt tttgaagtag
     4981 aatttctgcc cgatgccggc gcggaaacgc tcttcccccg tcgcgccgtc caaaatacgg
     5041 ctttgcacgg cggcggaaag gctgtttgcg gtgttaatcc tgtcgttgcc gtaatagagg
     5101 ttttcacgaa aaagctgccc gtagccgaag ctgctttccg acgaatcgaa attgggcagg
     5161 tcgttttggg atttggcagg aatatagttg tagaacaggc gcggctcgag ggtttgcagg
     5221 actccgccgc cgaacatccg cgtattgcgt tcgaaggtca tgccgctgtc gatgttgacg
     5281 atgggcagag tgcggctgac gcgtcgggct tcttggctgc cgaagcggtt gaggctgtaa
     5341 taggtggcgt gcagtccgag tttgggacgg acgtaacccc agctgttgct gaaatcccat
     5401 ttgatgtcgg gatagacgac gaggcggctg ccgtcttggc ggctgtcgtg gctgaagcgg
     5461 gtaaattggg cggacacgcc gatttgcgcc ctgccggtgt ttttgcgcca atcggcggaa
     5521 aggcgcggca tcagggcata cggtttgtct ttgtagccgc tttggtttgc cagcgtctgg
     5581 tatttcagaa ccgaaaggcc ggcattcagg ctgccgcccg ccgccctgcc gccataatcc
     5641 agccatacac ggcggttgag gttgacgttg ccggcgattt ctttgttgcc gtaaaagtcg
     5701 cggtagtagc cgctgtcgga gacttggttg aaatcgacac ccgcctgaag cgtgtcggaa
     5761 atgtcgtgcc ggtgctgcca tttcgcctga tagcggttat tcctgccgct tttcttgtcg
     5821 tgcggcagcc aggtcaggtc ggactggccg gcataatccg gccgcaggta gcgtacctgc
     5881 ccgtcaaaga ccgcgccgcg ttcgccgatc acgccgggcg cgaacgtggc atcgagattg
     5941 ggggcaaggt tgaaataata gggaacggaa agggaaacgc cgtccgaacc ggcggacagt
     6001 gagggaacga gcaggccgct tttgcggttg ccgtcaagcg ggaagtccgc ccaaggggtg
     6061 tagaaaatgg gaacgccgcc gaacacgaag gcggcgtgtt tggcaacgcc tatgcctttt
     6121 tcccgatcgg cttcgacgga agcggccttg acataccagc cggcatcgcc ggcggaacag
     6181 gtgttgaatt gggtttccgt cagtttgtaa tgcccttcgc ccaacatttc ggcggtgcgg
     6241 ctgacgcttt gcagccgccg tccgccgtgt tcggtttcca tacggacgtt gtgcgcttcc
     6301 ccggtctgct gctcgagatt gtaggtcagg gtttcgcccc gaatcagcgt accgtcctgt
     6361 tggagggcga accggtcgcc tgcggtaacg gtgtcgcccg actggtcgta atccgcccaa
     6421 tcggcattga gggtcgtccg attgcgttcg acgacgacgt tgccttcggc acgcacctgc
     6481 acctgcgact gtccttccat cctgtcggca acaatgcgcg tatagtcttc ggggatggat
     6541 gcttcgccgc tgccttggac ggcggcttcg gttctctcgg ggctgccgct ttcgttactg
     6601 caaaacaggc aggtcgaacc gaggctcagg ctggtaggct gtatgggttc ggacacgctc
     6661 cgaacgcttc ctccggcggt cggattgtcc gtttcctccg ccgcaacggc atcggcggcg
     6721 gcgcaatgcg tgccgaaaca gaagcccaat gccagcacca gtggtttgag tgaaaataaa
     6781 cgagccaaaa tcgcccctca agtcggtttg ccagttagaa tagcgtttat tgtaacctga
     6841 aatgctttag tactgttatg caacggcaaa ccgaactgaa aaattggctt cagaccatct
     6901 atcccgaacg ggacttcgat ctgtccttcg cggcggcgga tgctgatttc cgccgctatt
     6961 tccgtgcaac gttttcagac ggcggcagcg tcgtctgtat ggacgcgccg cccgacaaga
     7021 tgagtgtcgc gccttattta aaagtgcaga aactgtttga catggtcaat gtgccgcaga
     7081 tattgcacgc ggacacggat ttggggtttg tggtattgaa cgacttgggc aatacgacgt
     7141 ttttgaccgc gatgcttcag gaacagggcg aaacggcgca caaagccctg cttttggagg
     7201 ccatcggcga gttggtcgaa ttgcagaagg cgagccgtaa aggggttttg cccgaatatg
     7261 accgtgaaac gatgttgcgc gaaatcaacc tgttcccgga atggtttgtc gcaaaagaat
     7321 tggggcgcga attaacattc aaacaacgcc aactttggca gcaaaccgtc gatacgctgc
     7381 tgccgcccct gttggcgcag cccaaagtct atgtgcaccg cgactttatc gtccgcaacc
     7441 tgatgctgac gcgcggcaga ccgggcgttt tagacttcca agacgcgctt tacggcccga
     7501 tttcctacga tttggtgtcg ctgttgcgcg atgcctttat cgaatgggaa gaagaatttg
     7561 tcttggactt ggttatccgc tactgggaaa aggcgcgggc taccggcttg cccgtccccg
     7621 aggcgtttga cgagttttac cgctggttcg aatggatggg cgtgcagcgg cacttgaagg
     7681 ttgcaggcat cttcgcacgc ctgtactacc gcgacggcaa agacaaatac cgtcccgaaa
     7741 tcccgcgttt cctcaattac ctgcgccgcg tatcgcgccg ttatgccgaa ctcgccccgc
     7801 tctacgcgct cttggtcgaa ctggtcggcg atgaagaact ggaaacgggc tttacgtttt
     7861 aaacccaatc aaaatgccgt ctgaaaacca agtttcagac ggcattttcc aaacgggctt
     7921 agtgcgcggc tttttgttct tcgcgtactt tgtccgccaa aaggtcgatg gtgttcatac
     7981 cggactccca gtcggcaaat tcgactttat atttgccgcc gacgataacc gtgggcgtac
     8041 cgtcgatttg gaaggtttcg gtcagctcct gcattttgtc ggcgcgcgcc tggctttcgg
     8101 gagattcgta agcggcaagg acttttttgc cgtcaaaggc ggtttgttcg cccagccatt
     8161 ttttgaggac ttccggctct tgcagcttga ttttttggtt gaccatcgca tcgaaaatat
     8221 ggctgttcgc cacatctttg ctgtcggcgg cagccatatc gacggcggcg gcgaggcgtg
     8281 cgagcgtcag catttctttc tgccagacga cgtgttcggt acgcaggtac atatcgtctt
     8341 taaaagactt ggcgtgtttg cttaaaacag gttcgaggtg ggcgcagtgc ggacagaaat
     8401 agccgaaaaa ctcaaggact tcgactttgc ctgcctgctg ttggggaatc gggttggcaa
     8461 ggacagtata gttttgccct tcgaccagcc ctgccggggc ggcggctgcc gaagcggcag
     8521 gcgcgctgtc ggcggggacg ctggtttgga ctttgctgtc gcacgcggca agtgcgaaca
     8581 gggcggcaac gccgagggcg aggtgtttgg atttcatacg gctctccgtg atgttggaaa
     8641 cagatgttgg aaatcaatcg gaatccggct attttattgc atttccgcgt gttgatacag
     8701 tttgcctccg gaaaaggacg ttttcgtttg gggaaaccgc ttcggacggc atcaaacccg
     8761 atgccgtctg aagcggtttc tgtcgtacaa tacgcgccgt cgccccagac ggatacggct
     8821 gttggggaac taatgatgtt ccaacacaca ggacggcaca taaagcaccg ccctatgcgt
     8881 tgccctggtt cggaagggtt acaccccttt c
//
LOCUS       DAWKOG010000076         8703 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.076, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000076 DAWKOG010000000
VERSION     DAWKOG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8703
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.076"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(171..863)
                     /locus_tag="WJK66_001283"
     CDS             complement(171..863)
                     /locus_tag="WJK66_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ7578996.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGTPEYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(971..2164)
                     /locus_tag="WJK66_001284"
     CDS             complement(971..2164)
                     /locus_tag="WJK66_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ7578997.1"
                     /translation="MSNQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(2293..3030)
                     /gene="pxpA"
                     /locus_tag="WJK66_001285"
     CDS             complement(2293..3030)
                     /gene="pxpA"
                     /locus_tag="WJK66_001285"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEZ7578998.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGQVKAVDGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            complement(3017..3946)
                     /locus_tag="WJK66_001286"
     CDS             complement(3017..3946)
                     /locus_tag="WJK66_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEZ7578999.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKM
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDYLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQAYLNQIRRITHEAG"
     gene            complement(3943..4590)
                     /gene="pxpB"
                     /locus_tag="WJK66_001287"
     CDS             complement(3943..4590)
                     /gene="pxpB"
                     /locus_tag="WJK66_001287"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEZ7579000.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            4774..6981
                     /gene="uvrD"
                     /locus_tag="WJK66_001288"
     CDS             4774..6981
                     /gene="uvrD"
                     /locus_tag="WJK66_001288"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEZ7579001.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQTGQASVHSIMAVTFTNKAAKEMQTRLGAMIPVDVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDGGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTSRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     NAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTIENLQTASNEQGITLWQAACNAGAK
                     ATKVAAFVRLIEALRNQVGQMPLPEIIVGILKDSGLTEHYKTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFEILPENISDDPAFPILAFLSNAALESGENQAGADEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKTAYDSYGSPRQTAASKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEAM"
     gene            7216..7674
                     /locus_tag="WJK66_001289"
     CDS             7216..7674
                     /locus_tag="WJK66_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ7579002.1"
                     /translation="MSQVFKDFDLSSVWESDSWADENYKEAPFTPEILAAVESELGYK
                     LPQSFIELMAVQNGGIFVKNCFPTTQRNSWAKDHVQICEVSGIGFEKEGSLCGETGQK
                     LWLEEWEYPPIGVYFANDPSGGHALFALDYRECGRDGEPKVVFVEQESDF"
     gene            complement(7945..8160)
                     /locus_tag="WJK66_001290"
     CDS             complement(7945..8160)
                     /locus_tag="WJK66_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579003.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNPQSVQELEQHLIEAYDLMDTTG"
     gene            complement(8168..8371)
                     /locus_tag="WJK66_001291"
     CDS             complement(8168..8371)
                     /locus_tag="WJK66_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579004.1"
                     /translation="MLKRNLGFDTFKLITIFFLGIRRLQSKHIRAIWDDKSGAIVIRD
                     PNSKDGGTAFRPTLGKTYFDKQK"
     gene            complement(8284..8643)
                     /locus_tag="WJK66_001292"
     CDS             complement(8284..8643)
                     /locus_tag="WJK66_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ7579005.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
BASE COUNT         2171 a         2412 c         2163 g         1957 t
ORIGIN      
        1 tttcagttac agcagcttga tccctttccc ttatccaacg ggggaaggct aggatagggt
       61 ggcttgcaaa tatacagaac aagggacaag agccaccctc tctccaaccc tctccctccg
      121 tacgggaggg ggtggattct cgcgggcgaa gcccacgcta cggttagcct ttaccccagc
      181 acaaacaatt cccgcccgtg cgccttcagc caacttttag cattgtcggt atgcggcgtc
      241 agcgtgttca ccaaatgcca aaagcgcgga ctgtggtcgg ggtggcggag gtggcagagt
      301 tcgtggatgc agacatagtc ggcgacgtat tcgggcgtgc cgatcagccg ccagttgagg
      361 cggatgccgg tgtgcgggcg gcatacgccc caaaaggttt tggcgttgct caggtctgtg
      421 gcggtgggcg tcagtcctgt ttcggctgcg tgtttttcaa ggcggggcag caggtattcg
      481 cgggcgcgtt cgttcaacag gcggcgcagg tggtcgattt gtgcggcggt ttcttttcgg
      541 ggaagcagga tttcagacga cgtgatacgg atatggcttt ggctgtgggt atccagcttg
      601 gtctttattc cccgatacca aatccactcg ggtaagtttg ggtgggaaac aggatgcacg
      661 ggcgttttgg caagcgtgtt ccgcaaaatc gtttcgtttg ccgccagcca gtttgctaac
      721 gcgtggtctt gaaaaaaggg tgggacgttg atgctgaccg tctgcatatt gacggggcgc
      781 agaatcagat ttttcttggc actgcgtttg agttcgattt cgatgcacaa accgtcggaa
      841 agagtatagg tgaagcgttt catagttgtg aatgggtttc agaccggata catcgtctga
      901 aacaggaatt ttccatatca ggcggcaaac ttcggataat atacaaaatc aaacatctgc
      961 gctacaaggt tcagccgaac aagccgccga tatatttgct gatggtgatg gcgctgagta
     1021 ctgccatcaa accgaccaca atcacgccgg aaacggtgag ccacagcggg tgtttgtagt
     1081 cgccgacaat tttggttttg taggcggcaa tcagaatcag accgagggaa atcggtaaaa
     1141 tcaggccgtt taatgcgcct acgaacacca gcacctgcgc cggtttgccg atggtggaaa
     1201 atacggcggt ggacacggcg ataaaggcaa taatccattt gtttttattg cgttcgatag
     1261 acgggctgag accggagaag aacgacaccg aagtataagc cgcaccaatc accgaagtaa
     1321 tcgaagccgc ccaaatcacc acgccgaaaa tcagcaggcc gatgtatccc gccgcatatt
     1381 caaacggtgt ggaagcaggg ttgtcgggat tgagctgtac gccttggctg accacgccca
     1441 aaaccgccaa aaacaataca atccgcataa tcgaggcaat caggatcgcc cgcaccgagc
     1501 tttggctcac ttccggcaac gccgatttgc ctttgatgcc tgcgtccagc agacggtgcg
     1561 caccggcgaa ggtgatgtag ccgccgaccg tgccgcccac cagtgtaaca atcgccattg
     1621 catcgagttt ttccggcata aaggtatgca cggcggcatc tgccagcggc ggattcgcct
     1681 gccatgccac ataaaccgtc agcgcaatca ttacgaaacc catcacttgg gcgaatttgt
     1741 ccatcacttt gcctgcttct ttaaacagaa acacaccgat ggcaatcacg ccgctgatca
     1801 cggcaccggt ttccggcgac agtccggtca gcaggttcag acccaagcct gcgccgccga
     1861 cgttgccaat attgaacgcc aaaccgccca tcacaatcag cacagccaag aaatagcctg
     1921 cgccgggcaa gacctgattg gcaatatcct gcgcctgttt ttcggaaacg gcgacaatcc
     1981 gccaaatatt gagctgcgcc ccgatgtcga gcagaatcga gagcagaatc acaaagccga
     2041 aacttgccgc cagtgcttgg gtgaaggtgg cggtttgggt cagaaagccc gggccgatgg
     2101 cggaagtcgc catcaggaat gcagcgccga ttaaggcatt tctgcggttt ttttgattag
     2161 acataatcgc ttatcctcta taaaattggt tgttgctgtg tttgggcgaa acctgcggtt
     2221 ttagctacgc agaaactcgc tttgctcgtt ttggcgaaac ctgcggtttt cagacggcct
     2281 atgaactgtt tttcaagcag aaactttgat gcctgccgcc agtagttcct gccggatttt
     2341 ttcggcaaac accacggcgt gcggcccgtc tccgtgcaga cagatgctgt cggcttgcac
     2401 ggcaaccagg ctgccgtcca ctgctttgac ctgcccgtcc cgcaccatct gcaatacttg
     2461 ggcgatggct tcttcgtcgc tgtccacctg cgcatcgggg cggctgcggg gaaccagcgt
     2521 accgtcgggc atatagcggc ggtcggcgaa tacttcggaa atcacaccca agcctgcggc
     2581 ttttccggct tccaagagca ggctgccgga aagtgccatc aatttcaatt tcgggtcgaa
     2641 atccgccaca attcgggcaa cggtatccgc cagcgcacgg tttttcgccg cttggttgta
     2701 cattgcgccg tgcggtttga cataagccat ttccaaaccc tgatcacggc acaaggcctg
     2761 caatgcgccc aactggtaat tcagacacgc ccgcaaatcg gcttcggaca gattcatttc
     2821 ggtacggccg aagttttccc gatcgggata gccggggtgt gctccgatgc gcacgccgtt
     2881 ttgttgggca tacgccaatg ccgcccgaat atcggcaatg ctgccggcgt gttgggcgca
     2941 ggcgatgttg gccgaagtaa tcagctgcaa caaggcttcg tcgctgccgc agccttcggc
     3001 gagatcggcg tttaaatcaa cctgcttcat gggtgattct ccgtatttgg ttcagatagg
     3061 cttggttttt gcgccgcagg gcggtggctt ctttcaagcc gattattttg aatttgactt
     3121 tgctgccgaa gcgcacctgt gccagcctgc ccaaatcggc ggcggcaacg gtagcgattt
     3181 tcggataacc gccggtggtt tgcgcatcgg ccagcaggat aatcggtttg ccgccgggcg
     3241 gcacctgcac ggttcctgcc tgaacagcgt gggacagcat ttccaaaggt tgcgacaggg
     3301 tcagcggctg tccgtcgaag cggtagccca tgcggttgct atcgctttgc agcgtccacg
     3361 tttcccgttc cagattcaga cgcccttttt cactgaaagc ggcatattcc gacgaaggaa
     3421 caaggtggac ggtatcggta aacggtatcg gggcaatgcc gactttggac aattcctgcg
     3481 cacctttgcc gatggggaga taatcgcctt tttgcagcat tctgccctga tggccgccga
     3541 aaccggcttt caggtcggta cttctcgaac ccatcacttc cggcacatca aatccgcccg
     3601 ccacgcacac atagccgtac atgccctgca cggcacgcac cattttcaag gtctgccctt
     3661 tgcgggcggt ataacgccaa tacgaataga ccggttcgcc gtccaattcc gcctgataca
     3721 cggcaccggt gagacaaaac ggcgtatccc gttcaaacac cagcattatc ccgcccaaag
     3781 cgatttcgat tgcggccgtg ccttcgtcgt tgcccaataa aatattgccc gccgccaaag
     3841 caaccgtgtc catcgcaccg gcatgaccga tgccgtaacg ccggtgtccg tagcgtccgg
     3901 tatcctgaat atgcgccggt gcctgcactg ccgaaacgtg aatcatggct caatcctttc
     3961 tgcaacaaag cggacttggt cacccgccgc cagcagggtc ggcggattca aatcggctcg
     4021 gaacaagggt aattcggttc tgccgataat ctgccagccg ccgggcgaag cgaacggata
     4081 cacaccggtc tgactgccgc cgataccgac cgaaccggca ggaacggacg ttctcggcac
     4141 ggcacggcgg ggcgtgtgca atgcttcggg caagccgccc agataaggga aaccgggctg
     4201 gaagcccatc ataaatacgg tataagtttg cgccgtatgg cggcggacga tttcggaaat
     4261 aaccgtctga tggaaagcag cgacttccgc caaatccggg ccgtattcgc cgccgtagca
     4321 gacgggaatt tccaccagtt tgccctgatg gtctgtaacg gcggtgtgtt cccacacata
     4381 ttgcaattca tcggcaagcg tcgccaaatc ggtatcgaaa cgggtgaaca cggtcagatt
     4441 gttcatgccg accaccactt cctcaatcct gtcgtgctgc ccgagcgcag cggcaaacgc
     4501 ccacaacttt tgctgtttgc ccagttcgga aggtgcattc agtcggcaga ccaaagcgga
     4561 ttcgctgatt ggtgtgatct ctattctcat ttgttgttca ttttggttat gttttaatga
     4621 atctatatgc aggggcggcg gtttgtcaat atcttctgtg ctgcatcatc aaaccgtcga
     4681 ttggaaaagt gctgccctgc cgctgcactt tttcagacga ccttaaaccg tttctattaa
     4741 aatagcgcat tccacttttc agacggcatc tctatgtttc ccgaccaatc cgcccccaac
     4801 ctgctgcaag gcttgaatcc cgaacaactt tccgccgtaa cctggccgcc gcaatccgcc
     4861 cttgtgctgg caggcgcggg cagcggcaaa acgcgcgtgc tgaccacgcg tatcgcatgg
     4921 cttttgcaaa ccggtcaggc aagcgtgcac agcattatgg cggtaacgtt taccaacaaa
     4981 gccgccaaag aaatgcaaac ccgactcggc gcgatgattc ccgtcgacgt ccgcgctatg
     5041 tggctcggca cgttccacgg cctctgccac cgctttctac ggctgcacca ccgcgatgcc
     5101 ggtttgccgt cttcctttca aatcctcgac ggcggcgacc agctttccct catcaaacgc
     5161 ctgctcaaaa gcctcaacat cgccgaagaa atcatcgcgc cgcgttcgct gcaaggcttt
     5221 atcaacgcgc aaaaagaatc cggtttgcgc gcttccgtct tgagtgcgcc cgatccgcac
     5281 acaagccgca tgattgaatg ctacgccgaa tacgacaaaa tctgccaacg cgaaggcgtg
     5341 gtcgattttg ccgaactcat gctccgcagc tacgaaatgc tgcaaagcaa cgaaatcctg
     5401 cgccagcatt accaaaaccg cttcaaccac attctcgtcg acgagttcca agacaccaac
     5461 aaactgcaat acgcctggct caaactcatg gcgggcggca acgcggcagt gtttgccgtc
     5521 ggcgacgacg accaaagcat ctaccgcttc cgtggcgcaa acgtcggcaa tatgaccgcg
     5581 ctgatggaag agttccatat caacgcgccc gtcaaactcg aacaaaacta ccgctctgtc
     5641 ggcaacatcc ttgccgccgc caatgccgtt atcgaaaaca acgacgaacg actcggcaaa
     5701 aacctgcgca ccgacgccga agcaggcgac aaaattcgct actactccgc ctttaccgac
     5761 ctcgaagaag cccggttcat cgtggacgaa accaaagccc tcgaacgcga aggctgggat
     5821 ttggacgaaa tcgccgtcct ctatcgcagc aacgcccaat cccgcgttat cgaacaaagc
     5881 ctgttccgca gcggcatccc ttacaaaatc tacggcggcc tgcgctttta tgaacgccaa
     5941 gaaatcaaac acgcgctcgc ctaccttcgc ctcgccgtta atcccgacga cgacaacgcc
     6001 ctcttgcgcg tcatcaactt cccgccgcgc ggcatcggcg cacgcaccat cgaaaacctt
     6061 cagacggcct caaacgaaca aggcatcacc ctttggcagg cagcctgcaa cgccggcgca
     6121 aaagccacca aagtcgccgc cttcgtccgc ctcatcgaag ccctgcgcaa ccaagtcgga
     6181 caaatgcccc tgcccgaaat catcgtcggc atcctcaaag acagcggcct gaccgaacac
     6241 tacaaaaccc aaaaaggtga caaccaagac cgcctcgaca acctcgacga actcgtcaac
     6301 gccgccatcg agttcaaacc cgaagacagc aactttgaaa tcctgcctga aaacatttca
     6361 gacgaccccg cctttcccat cctcgccttc ctaagcaacg ccgccctcga atccggcgaa
     6421 aaccaagcag gcgcggacga aaaagccgtc caactcatga ccgtccacgc cgccaaaggc
     6481 ttggaattta acgccgtctt cctcaccggc atggaagaag gccgcttccc cagcgaaatg
     6541 agtctcgccg aacgcggcgg cctcgaagaa gaacgccgcc tcatgtacgt cgccatcacc
     6601 cgcgcccgca aacgcctcta catcaccatg gcgcaacaac gcatgctgca cggacaaacc
     6661 caattcggca tcgtctcccg cttcgtcgaa gagatcccac ccgaagtatt gcactacctg
     6721 tccgtcaaaa agaccgccta cgacagctac ggcagcccgc gccaaaccgc cgcatccaaa
     6781 gacaaaatca tcgacgacta caaacagccc caaacctacg caggtttccg tatcggacaa
     6841 aacgtccgcc acgccaaatt cggcaccggc gtgattatcg atgccgcaga taaaggcgaa
     6901 tccgcccgac tgaccatcaa cttcggcaaa cagggcgtga aagagttgga taccaagttt
     6961 gcgaaattgg aagcgatgta gaagggagat ggcagaggcc gtctgaaaac gggaacaaca
     7021 atttttgccc aaacctccgc cgtcattccc gcgcaggcgg gaatccagac ctttcggcac
     7081 gggaacttat cggataaaag gtttctttag attccacgtt ctagattccc gcctgcgcgg
     7141 gaatgacgga cggtaaaagc cgggtataga tacctaaccc ttgtttatag ctgaaaatca
     7201 ataaggaaga acattatgtc ccaagttttt aaagattttg atttgtcctc cgtatgggaa
     7261 tccgacagtt gggcagatga aaactacaaa gaagccccgt ttacccctga aattttggct
     7321 gccgtagaaa gtgaactggg ctataaattg ccgcaaagtt ttattgaatt gatggcggta
     7381 caaaacggcg gaatatttgt caaaaactgt tttccgacca cacagagaaa ttcgtgggcg
     7441 aaagatcatg tgcaaatttg cgaggtatcg ggaatcggtt ttgaaaaaga aggaagcctg
     7501 tgcggcgaaa cagggcaaaa actttggctg gaagaatggg agtatccgcc catcggtgtg
     7561 tattttgcca acgacccgtc aggcggtcat gccctatttg ccttagacta tcgggagtgt
     7621 ggaagagacg gagagccgaa agtggtgttt gtcgaacaag aatcggattt ttaaatcgtc
     7681 gaacttgccc ccgattttga aacctttatt cacagcttgc ggcatgaaga tgagtttatt
     7741 gacgaggaaa tataaaacgg cggttgaaaa accagaatca tcaaaggaaa acgggcggaa
     7801 taacagataa ccgattgaat ccgtaaggaa aacggtttgt aggaacgtgc catccaagac
     7861 ctttgcaaag aaaaactgtc cccgacagca ttgacattat taacagaact tatcaatttt
     7921 ggagctatct caaatataat tcggttatcc tgttgtatcc attaaatcat atgcttcaat
     7981 taaatgttgt tctagctctt gcactgattg aggattgcga attcctgata caatctcaaa
     8041 ttcttctgca tctatgcaaa cacctgcata aatttcaata acaagggaac gcaataattg
     8101 aagctcttct cttgttaaag aaataataat gtcatcacct gtgtaattga ttatattcat
     8161 aataatttta tttttgtttg tcaaagtaag ttttgcctaa ggttggtcta aatgcagttc
     8221 caccatcttt tgaatttggg tctctgatta caattgctcc agacttatca tcccaaattg
     8281 ctcttatgtg tttggattgt aatcttcgaa ttcccaagaa aaaaatcgta ataagtttga
     8341 aagtgtcaaa tcccaagttt cttttgagca atattctaat attttatcaa tttcactttt
     8401 aataatctta tgatcaaact gttctaatat taatgcatta gaccaaaaaa aaccttcttt
     8461 attacaatga tgggaaatcc atttaggaga acaaatgcaa agtgaaaaaa tagatgagcc
     8521 ttgttctcct tcgattccga tatccaaatc tatccatcta tggaaattat ctggaatttc
     8581 gggggtaaat ttttcaaaat caatatcata taaatttatg ctttttaaat ccaatttaat
     8641 cattagggct gtcctagata aatagggaaa ttcaaattaa gttagaatta tccctatgag
     8701 aaa
//
LOCUS       DAWKOG010000077         8696 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.077, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000077 DAWKOG010000000
VERSION     DAWKOG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8696
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.077"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            33..1859
                     /gene="mafB"
                     /locus_tag="WJK66_001293"
     CDS             33..1859
                     /gene="mafB"
                     /locus_tag="WJK66_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HEZ7579006.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGNIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPVPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGAMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFTAVIPIKGIGAVRGKYGLGGI
                     TAHPVKRSQMGAIALPKGKSAVSNNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLADQRHPKTGVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VFDAKPRWEVDRKLNKLTTREQVEKNVQEIRNGNKNSNFSQHAQLEREINKLKSADEI
                     NFADGMGKFTDSMNDKAFSRLVKSVKENGFTNPVVEYVEINGKAYIVRGNNRVFAAEY
                     LGRIHELKFKKVDFPVPNTSWKNPTDVLNESGNVKRPRYRSK"
     gene            1862..2326
                     /locus_tag="WJK66_001294"
     CDS             1862..2326
                     /locus_tag="WJK66_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579007.1"
                     /translation="MAIWNYRYYLIPSAAIRNKFNANKDIILDEYRSNGFQNFNENKS
                     FENYFIDNDVILLSIINEAKKQLKLKESWDKDAIMFCDNFGNSLTVWPDDIECELDLR
                     FDYTKFIQKTIDWAIKYNCLLVIEKTGNVVSPNINNLMYEIKAYLESKPWPI"
     gene            2515..2718
                     /locus_tag="WJK66_001295"
                     /pseudo
     CDS             2515..2718
                     /locus_tag="WJK66_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223961.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2709..2996
                     /locus_tag="WJK66_001296"
     CDS             2709..2996
                     /locus_tag="WJK66_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEZ7579008.1"
                     /translation="MQLEIIGSKIYTEQDFHNQISKIFSIQDYYGNNLDALWDLLSTN
                     VERPITLVWKDAMFSKNKLENIFIEIVNVLERVKKQDEDYGFEEKFNYILE"
     gene            3492..4748
                     /locus_tag="WJK66_001297"
     CDS             3492..4748
                     /locus_tag="WJK66_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HEZ7579009.1"
                     /translation="MKSKSGVIASLTGAAVGGTPVDAQTGGAVGQNAVENNLYLTSEA
                     LKKDKQTARKIYSVIKEQVKHECSSTGRITECRQNIGRIIEFTQDKRFDSRFKDLKKE
                     SLYYLNKHPDLVASYLKAEYEKLDREDKSILHRYISPGAEIVSGSLGVVLSGVAGGGS
                     CAETFGLGCAAALVGVTSSYDHVITGTKNFGKKASEQRPTIAVQALKQLGLSEQAAEY
                     VQFSIDLFSVGKSGGGIPKAKPVFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQS
                     SQFKAHAQREWENKTGLDFNHFIGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGV
                     YQATVEIKKPDGSWEVKTKKGGKVMTKHTMFPKDWDEARIRAEVTSAWESRIMLKDNK
                     WQGTSKSGIKIEGFTEPNRTAYPIYE"
     gene            4779..5198
                     /locus_tag="WJK66_001298"
     CDS             4779..5198
                     /locus_tag="WJK66_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579010.1"
                     /translation="MKRIKCFCDKFPSGDTFRMCIILDDYDNRVDYYVGIYDYITSTL
                     MSDIYYRSTIDEHFKIIELIENNPNEIYDDGGGQQFCLEFHHDKVIFYHNEFDEEDGY
                     PVLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            5235..6209
                     /gene="mafB"
                     /locus_tag="WJK66_001299"
                     /pseudo
     CDS             5235..6209
                     /gene="mafB"
                     /locus_tag="WJK66_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
     gene            6220..6681
                     /locus_tag="WJK66_001300"
     CDS             6220..6681
                     /locus_tag="WJK66_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579011.1"
                     /translation="MNLETAENIQCEFEYLALDGYFPMHFASHGQGNKDWQFAVEFIY
                     RLLICQLATLEPINFETKNDILDFCHNLAKQSPFNNDNEVWYQGEIVLTKKGIDLIKE
                     YIPEAFEEWNGKKFELNIPFIKTLKNIFVNYEVAWDENNPLFPIISLNLGT"
     gene            6707..6955
                     /locus_tag="WJK66_001301"
     CDS             6707..6955
                     /locus_tag="WJK66_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HEZ7579012.1"
                     /translation="MEIKKPDGSWEMKKGRGGKPMTNHSMFPKDWDEARIRAEVTSAW
                     EKRKELEGQKWRGTSKSGVEIEGFTEPNRTAYPLYNSK"
     gene            6982..7401
                     /locus_tag="WJK66_001302"
     CDS             6982..7401
                     /locus_tag="WJK66_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579013.1"
                     /translation="MYKIKCFYDQFSSGELFNYCQFLDNSSNQKINTRGTVYQYIIYV
                     LTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYHNEFDEEDGW
                     PDLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            7479..7562
                     /locus_tag="WJK66_001303"
                     /pseudo
     CDS             7479..7562
                     /locus_tag="WJK66_001303"
                     /inference="COORDINATES: protein motif:HMM:NF026862.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7559..7801
                     /locus_tag="WJK66_001304"
     CDS             7559..7801
                     /locus_tag="WJK66_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein 31"
                     /protein_id="HEZ7579014.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            7879..8124
                     /locus_tag="WJK66_001305"
     CDS             7879..8124
                     /locus_tag="WJK66_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ7579015.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PDYTWHHHQDTGRMQLVPEWEHSKTGHIGGTAMDKGK"
     gene            8127..8513
                     /locus_tag="WJK66_001306"
     CDS             8127..8513
                     /locus_tag="WJK66_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579016.1"
                     /translation="MWKIYKENSTDLNFAIGSIYCQAINLTEFKMWVEKIIREVDLDE
                     IPNYFFDLIDFQSLYDLIDIIGFVPENNLSKNQDNALTGIAFLRGIDVYDPPISKEKA
                     LKALKKHPEIYQRFQHFFPFVELPPL"
BASE COUNT         2915 a         1596 c         1934 g         2251 t
ORIGIN      
        1 tttcattccg taccatctta aggaacatca aattgggcat ttcccgcaaa atatccctta
       61 ttctgtccat actggcagtg tgcctgccga tgcatgcaca cgcctcagat ttggcaaacg
      121 attcttttat ccggcaggtt ctcgaccgtc agcatttcga acccgacggg aaataccacc
      181 tattcggcag caggggggag cttgccgagc gcagcggcca tatcggattg ggaaacatac
      241 aaagccatca gttgggcaac ctgatgattc aacaggcggc cattaaagga aatatcggct
      301 acattgtccg cttttccgat cacgggcacg aagtccattc ccccttcgac aaccatgcct
      361 cacattccga ttctgatgaa gccggtagtc ccgttgacgg attcagcctt taccgcatcc
      421 attgggacgg atacgaacac catcccgccg acggctacga cgggccacag ggcggcggct
      481 atcccgttcc caaaggcgcg agggatatat acagctacga cataaaaggc gttgcccaaa
      541 atatccgcct caacctgacc gacaaccgca gcaccggaca acggcttgcc gaccgtttcc
      601 acaatgccgg cgctatgctg acgcaaggag taggcgacgg attcaaacgc gccacccgat
      661 acagccccga gctggacaga tcgggcaatg ccgccgaagc cttcaacggc actgcagata
      721 tcgtcaaaaa catcatcggc gcggcaggag aaattgtcgg cgcaggcgat gccgtgcagg
      781 gtataagcga aggctcaaac attgctgtta tgcacggctt gggtctgctt tccaccgaaa
      841 acaagatggc gcgcatcaac gatttggcag atatggcgca actcaaagac tatgccgcag
      901 cagccatccg cgattgggca gtccaaaacc ccaatgccgc acaaggcata gaagccgtca
      961 gcaatatctt tacggcagtc atccccatca aagggattgg agctgttcgg ggaaaatacg
     1021 gcttgggcgg catcacggca catcctgtca agcggtcgca gatgggcgcg atcgcattgc
     1081 cgaaagggaa atccgccgtc agcaacaatt ttgccgatgc ggcatacgcc aaatacccgt
     1141 ccccttacca ttcccgaaat atccgttcaa acttggagca gcgttacggc aaagaaaaca
     1201 tcacctcctc aaccgtgccg ccgtcaaacg gcaaaaatgt caaactggca gaccaacgcc
     1261 acccgaagac aggcgtaccg tttgacggta aagggtttcc gaattttgag aagcacgtga
     1321 aatatgatac gaagctcgat attcaagaat tatcgggggg cggtatacct aaggctaagc
     1381 ctgtgtttga tgcgaaaccg agatgggagg ttgataggaa gcttaataaa ttgacaactc
     1441 gtgagcaggt cgagaaaaat gttcaggaaa taaggaacgg taataaaaac agtaacttta
     1501 gccaacatgc tcaactagag agggaaatta ataaactaaa atctgccgat gaaattaatt
     1561 ttgcagatgg aatgggaaaa tttaccgata gcatgaatga caaggctttt agtaggcttg
     1621 tgaaatcagt taaagagaat ggcttcacaa atccagttgt ggagtacgtt gaaataaatg
     1681 gaaaagcata tatcgtaaga ggaaataata gggtttttgc tgcagaatac cttggcagga
     1741 tacatgaatt aaaatttaaa aaagttgact ttcctgttcc taatactagt tggaaaaatc
     1801 ctactgatgt cttgaatgaa tcaggtaatg ttaagagacc tcgttatagg agtaaataaa
     1861 aatggcaatt tggaattatc ggtactacct tatcccatca gctgctatca gaaataaatt
     1921 taatgcaaat aaagatatca tacttgatga gtatcgatct aatggttttc agaattttaa
     1981 tgagaataaa agttttgaaa attactttat cgataatgat gttatattat tatcaataat
     2041 aaatgaagca aaaaaacagc ttaaattaaa agagtcttgg gataaagacg caatcatgtt
     2101 ttgtgataat tttggtaata gtcttaccgt ttggccagat gatatagagt gcgaacttga
     2161 tttaagattt gattatacta aatttattca gaaaaccatt gattgggcaa taaaatataa
     2221 ttgtctactt gtaatagaaa aaacaggaaa tgtagtttcc cctaatataa ataatctgat
     2281 gtatgaaata aaagcatatt tggaaagcaa gccgtggccc atatgaaacc taaactcaac
     2341 aagtaggatg tgtgcggaac gcacgtatgc ggttctcaag gtttgagcta agaggccgtc
     2401 tgaaaacaga aaaactgttt cagacgacct ttcttttaac cagttgccac agcaaccgga
     2461 caaaagcagc ctacctccac atccatatag gcaatacagg ggagatattt tgtaaattct
     2521 acgaatattt tacctgctaa acagggtagg atatggtatg aagcgaacat tggcttaata
     2581 aacactatgt caagatcgaa tcaggctggt actagattgt tgtattccaa tgatggattg
     2641 ctatatataa caactgatca ttatatctct gcaacacggt ttggagcttg gaaatgggaa
     2701 tataacttat gcaattagag attatcggta gtaaaattta tacggaacaa gattttcata
     2761 atcaaatttc aaaaatattt tctatacaag attattatgg gaacaatctt gatgctctgt
     2821 gggatttatt aagcacaaat gtagaacgac cgattacttt ggtatggaaa gatgctatgt
     2881 tctcaaaaaa taaattagaa aatatattta ttgaaatcgt aaatgttcta gaaagagtta
     2941 agaaacaaga tgaggattat ggattcgaag aaaaatttaa ttatatttta gagtaagtaa
     3001 accctaatta cattacgtac acaggcttaa aactccccag agccaattaa gcaagccgta
     3061 acccatataa aacttaaact caacaagtag catgtgtgcg gaacgtacgc atgcggttct
     3121 taaagtttga gctaagaggc cgtctaaaaa cagaaaaacc gtttcagacg gtctttgttt
     3181 aacgccaccg atccagcggg ttacaaagcg cagtcaatgc cgctgcgcct tatgcctccg
     3241 aagcaatagg cagaacattt ggacacggtg aaaacaaaaa cgaaaccgcc caagccgtcg
     3301 gacatttcct tttaggagca gctattgccc gcgtcaacgg tggtaatttt gctgccggcg
     3361 gctcggcagc agttgcagct gaaaaggcgg cggaacatct tgcccaacag tataacgacg
     3421 gtaaaaccgc aatcgatccg caaacaggcg agttcaatgc caacctgctg ccggaacata
     3481 tcaaagagga aatcaaatca aagagcgggg tgattgcatc gctgacgggc gcggccgtgg
     3541 gcggcacgcc ggtagatgcg caaaccggag gtgcggtcgg acagaatgcg gtggaaaaca
     3601 acctctatct gacatcggaa gccttaaaga aggacaagca gacagctcgt aaaatttatt
     3661 ccgtcataaa agagcaagtc aagcatgaat gcagttccac aggaagaatt accgaatgtc
     3721 gtcaaaatat aggacgcatt atcgaattta cccaagacaa acgctttgac agtaggttta
     3781 aggacttaaa aaaagaatcc ttatattacc taaataaaca tcctgattta gtagcctctt
     3841 atttgaaggc tgaatacgaa aagctggata gggaagacaa aagtatcctg caccgctaca
     3901 tctcacccgg ggctgaaatc gtttcgggca gtttgggggt tgttctttca ggagtagccg
     3961 gaggcggatc ttgtgccgag actttcggct taggctgtgc cgccgctttg gttggtgtaa
     4021 cgtcttccta cgatcatgtc attactggga cgaaaaactt cggaaaaaaa gccagcgagc
     4081 aacgaccgac gattgcggtt caggccttga agcagttggg gctgtcggag caggctgcgg
     4141 aatatgttca gttctctata gatttgttca gtgtgggtaa atcggggggc ggtataccta
     4201 aggctaagcc tgtgtttgat gcgaaaccga gatgggaggt tgataggaag cttaataaat
     4261 tgacaactcg tgagcaggtg gagaaaaatg ttcaggaaac gagaagaagg agtcagagta
     4321 gtcagtttaa agcccatgcg caacgagaat gggaaaataa aacagggtta gattttaatc
     4381 attttatagg tggtgatatc aataagaaag gcacagtaac aggagggcat agtctaaccc
     4441 gtggtgatgt acgggtgata caacaaacct cggcacctga taaacatggg gtttatcaag
     4501 cgacagtgga aattaaaaag cctgatggaa gttgggaggt gaaaacgaaa aaaggtggga
     4561 aagtgatgac caagcacacc atgttcccaa aagattggga tgaggctaga attagggctg
     4621 aagttacttc ggcttgggaa agtagaataa tgcttaagga taataaatgg cagggtacaa
     4681 gtaaatcggg tattaaaata gaaggattta ccgaacctaa tagaacagca tatcccattt
     4741 atgaatagta atatttatga aaaattagga gattaatgat gaaaagaatt aagtgctttt
     4801 gtgataaatt tccatcagga gatacattta gaatgtgtat cattctggat gactatgata
     4861 atagggttga ttattatgta ggaatatatg attacattac gtctacctta atgagcgata
     4921 tttactatcg atccacgatt gatgagcatt tcaagattat agaattaata gaaaataatc
     4981 caaatgaaat ttatgatgat ggcggtggtc aacaattttg cctagaattt catcatgata
     5041 aggtcatttt ttatcacaat gaatttgatg aagaagatgg ttatccagta ttaagctgtt
     5101 cgctgcatac ttttaaaact gctttaattg cttggaatgc ttttttgcaa ttgcctaaaa
     5161 gtattcattc ggtggtggag actgtgattg aggaataagc ataattagct taatgaatag
     5221 aatcagcgat atagaaaggt agaatcagat atatcccacc taaaaattac tctccttcag
     5281 caccgctacc aaaaggacct aataatggat atagtgccgg gaatttaagc aaaaaagtcc
     5341 tgactttagt ttacataaac ggcaaactca aattcgggcc aagcagtctg ccgtcttcgg
     5401 cattgaaagc acctgttccc attgtcggca aggctgaggc tccgcccgga aaagctgtgc
     5461 ctgtcggcga agcccgcatt ccttcaaatc caaaagcggt aaaaggaaca accgttaccc
     5521 tgtcgcagga acaacgccta gccaacggag agctgatacc gaaaggctct gtggtaacgt
     5581 ggtcggacaa tgcggtgaag gttgtgcggc ctgatgggaa aaagcggaga tttaatagtc
     5641 gggattggga acagaaggca ttgccgaaac cgcaactacc gaaagattac agagatgaca
     5701 tcaacatagg tggaaataat cctagattta atcagcaacg gcatgaaaaa atcgaatccc
     5761 acaacccaaa cagaatagag gagatacgcc aacgtaatgc cgtttcttct gcagaaaggc
     5821 aggctgatgc attaaaaagg atgcaaacgt ctggccgcac ccgcctgcct gggcagccac
     5881 aaaaaggaaa acccggcgct gctagagaaa tgtcctcttc agacaatccg aatcaaacag
     5941 ccgaggcttt tgcacgtcga tatttaggca ggaaacctac actaatggaa cgagtcgaag
     6001 gtgaaaaatt atacggaggt aaatgtgtgg gctgttggaa agcgcaaatg ccggacggaa
     6061 cctttgtggt gtataggcca gcaggtaatg cttctatgac caagggaact actgcgtctg
     6121 ttgatattaa taacccaaca ttaaataact taaatattaa tttaaaaggc aagccaacag
     6181 agttaaaatt taaattccct gctagataag gatgttttaa tgaatttaga gacagcagaa
     6241 aatattcaat gcgaatttga gtatttagca ttagatggtt attttccaat gcattttgca
     6301 tcgcatggtc aaggaaacaa agattggcaa tttgcagttg aattcatata tagattatta
     6361 atatgtcaat tagcaactct tgaacctatc aactttgaaa cgaaaaatga tattttagac
     6421 ttttgccata atttagcaaa acaaagtcct tttaataatg ataatgaggt gtggtatcag
     6481 ggggaaattg tattaacaaa aaaagggata gatttaataa aggaatatat tccagaggcg
     6541 tttgaagagt ggaatggaaa gaaatttgaa ttaaacattc ctttcattaa aacattaaaa
     6601 aatatatttg ttaattatga ggtagcatgg gatgagaata atccactatt ccccattata
     6661 tctttaaacc tcggcacctg ataaacatgg ggtttatcaa gcgacagtgg aaattaaaaa
     6721 gcctgatgga agttgggaaa tgaaaaaagg aaggggcgga aaacctatga caaatcattc
     6781 tatgttccca aaagattggg atgaggctag aattagggct gaagttactt cggcttggga
     6841 gaaaagaaag gaattagaag gtcaaaaatg gagaggtaca agtaaatctg gtgttgaaat
     6901 agaaggattt acggaaccta atcgaacagc ttatccactt tataatagta aatgaagtat
     6961 tgattaaaaa ggtgttttta aatgtataaa ataaaatgtt tttatgatca gtttagttct
     7021 ggtgaattat ttaactactg ccagttttta gataattctt ctaatcaaaa gataaataca
     7081 cgaggaacgg tgtatcaata tattatttat gttttaacag gagaccttta tctacaaaaa
     7141 gacatagatg aaaatttaga atttatccat caagcagaaa ataatcccaa tgaagtttat
     7201 tcaggaggtg gacaaggttt ttgctgggat atttctgctg aaaaagtagt tttctatcac
     7261 aacgaatttg atgaagaaga cggctggcct gatttaagct gttcgctgca tacttttaaa
     7321 actgctttaa ttgcttggaa tgcttttttg caattgccta aaagtattca ttcggtggtg
     7381 gagactgtga ttgaggaata agcataatta gcttaatgaa tagaatcagc gatatagatt
     7441 ggactgcaaa tccacgctta tacgctgtgc catgattaac aggaagagag caacttggat
     7501 gggctagtag ggatggtaag catttaaata tatcaattga tggaaagatt acacacaaat
     7561 gactaattta aaattagatt tctactctga agttattata aaagattctt gccctaatga
     7621 tttgttagaa aatggtgaga ctattaaagg aaaaaaagga gtggtattag gtataagcga
     7681 agaagatggt ataatctatg gctatactat tttacttttt gatataaaat attgtatata
     7741 tatagataag aaatatatta taccgactgg aaaaaaattc tctcgcgatg atttttatta
     7801 gctaatttaa gtaatttaag caagtttttt agatactgaa actggtaatt atgtttatgt
     7861 aagcataagg agttcagatt gaaggattct acagggcaaa tgaggctggc aaccaaggat
     7921 ttggcggaag ccattaaacg aggagaagta cgtagttctg cttttacaac aaagcaacta
     7981 aaggcaattg aaaaaggaga atctaaaata ccggattata cttggcatca tcatcaggat
     8041 actggacgta tgcagcttgt acctgaatgg gaacattcta aaaccggtca cataggaggg
     8101 acggcaatgg ataaaggtaa atgattatgt ggaaaattta taaagaaaat agcaccgact
     8161 taaattttgc cataggcagt atatattgcc aagcaatcaa tcttaccgaa tttaaaatgt
     8221 gggtcgaaaa aatcataagg gaagtggatt tagatgaaat tccaaattat ttttttgatt
     8281 tgatagattt tcaatcacta tacgatctga ttgatattat aggatttgtt cccgaaaata
     8341 acttatcaaa aaatcaggat aatgcattaa ccggtattgc ttttttaagg gggatagatg
     8401 tctacgatcc tcccatttca aaagaaaaag cattaaaagc cttaaagaaa catcctgaaa
     8461 tttatcagag gtttcagcat ttctttccgt ttgtagaact gcccccgctt taaacagtca
     8521 aaatgccgtc tgaaagccat ttccgccgct cagacggcat tttcgcccct tttgtttaca
     8581 aacccttaaa atccatttac actcaaaccc gttcaaaaca aaacaaaccc tgccatgaaa
     8641 accctgctcc tcctcatcac cctcgtccta accgcctgcg gcacactgac cggcat
//
LOCUS       DAWKOG010000078         8691 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.078, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000078 DAWKOG010000000
VERSION     DAWKOG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8691
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.078"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(334..1425)
                     /gene="ychF"
                     /locus_tag="WJK66_001307"
     CDS             complement(334..1425)
                     /gene="ychF"
                     /locus_tag="WJK66_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEZ7579017.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVAGRVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERAMLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LERLKELAAKENAPVVAVCAAMESEIAELEDDEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVISYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            complement(1539..3215)
                     /locus_tag="WJK66_001308"
     CDS             complement(1539..3215)
                     /locus_tag="WJK66_001308"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEZ7579018.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNADNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDAVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RLGNILVAYAKDGSPVYAKDLKAHGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLADLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLEALAKGLPNLLKHISNLKNVFGLPVVVAINRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLARKVVNAIENQTNNFGFAYDVELSVKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RIAVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDEHGVIHGLF"
     gene            3390..3821
                     /locus_tag="WJK66_001309"
     CDS             3390..3821
                     /locus_tag="WJK66_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ7579019.1"
                     /translation="MSSNAWLFWALASAGFASLTAIFAKMGLQGIDSDFATFIRTLVI
                     LAALLLFLTYTGKWQGVNGFTGHNWTFLILSGLATGASWLAYFKALQLGNASQVAPVD
                     KFSLVLVALMAVVFLDERPNTQEWIGLGLVTAGVLVLALKR"
     gene            complement(4066..4761)
                     /locus_tag="WJK66_001310"
     CDS             complement(4066..4761)
                     /locus_tag="WJK66_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEZ7579020.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEDALGDGSAYGVNIAYSPEPEGGLETAGGIARALPLLGDTPF
                     LVVNGDVLTDIDFTAAFQTASSLPEHISAHLWLVENPPHNPDGDFSLLPDGSVQSEVS
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRGEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(4779..5279)
                     /gene="hpaC"
                     /locus_tag="WJK66_001311"
     CDS             complement(4779..5279)
                     /gene="hpaC"
                     /locus_tag="WJK66_001311"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HEZ7579021.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINTLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRSLD"
     gene            complement(5394..5834)
                     /gene="nadR"
                     /locus_tag="WJK66_001312"
     CDS             complement(5394..5834)
                     /gene="nadR"
                     /locus_tag="WJK66_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HEZ7579022.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            complement(6177..6686)
                     /locus_tag="WJK66_001313"
     CDS             complement(6177..6686)
                     /locus_tag="WJK66_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HEZ7579023.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWVRKPDEPAGAAGRPLT
                     LLSGQRLTLGQFSRDRTVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSSA
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            complement(6765..6840)
                     /locus_tag="WJK66_001314"
     tRNA            complement(6765..6840)
                     /locus_tag="WJK66_001314"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6805..6807),aa:Lys,seq:ttt)
     gene            7051..8130
                     /gene="apbC"
                     /locus_tag="WJK66_001315"
     CDS             7051..8130
                     /gene="apbC"
                     /locus_tag="WJK66_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEZ7579024.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIAAAIADSVQETLMPETGDTHIHLSMDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVDDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTDQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPAQLFDEHPAIAKIYTDA
                     AFQIALSIADKGKDFSSRFPKIVVE"
BASE COUNT         2040 a         2505 c         2198 g         1948 t
ORIGIN      
        1 aagttaatcc actatactcg aaacgcggcg gcgcaaatgc cgtctgaaag gtcattttgt
       61 acgggatatt cggaatgccg gacggctttt tgcaacagcg gggcaggcgg tttgttttgc
      121 aggaatcggg gagggcaaat cggaaatgcg ggtgggattt tatagtggat taacaaaaac
      181 cggtacagcg ttgccccgcc ttgtcctgat ttttgttaat ccgctatatt ttgatacggc
      241 tgcattccgg cggtatggaa acgccgaaaa ccaccaaaac cggcttcaga cggcatttct
      301 ggcaaagccg tctgaaaacc tgccgcattt gggttacacg ttaaacaaaa agtgcatcac
      361 atccccatcc tgcacgacgt attccttgcc ttccacacgc attttgccgg cttctttggc
      421 tttggcttcg ccgccgagcg agacaaagtc gtcgtaagaa atgacttggg cgcggatgaa
      481 gccgcgctca aagtcggtgt gaatcacgcc ggcggcttgc ggggcggtgt cgcctttgtg
      541 tatcgtccaa gcgcggactt ctttcacgcc ggcggtaaag taggtttgca gccccaaaag
      601 gtcgtagccg gcgcggatca ggcggttcag gcccggttct tccaagccca tttcggcgag
      661 gaactcggct ttttcgtcgt cttccaattc ggcgatttcg ctttccattg cggcgcaaac
      721 ggcgacgacg ggggcgtttt cttttgccgc caattctttc aggcgttcga ggtgcggatt
      781 gttttcaaaa ccgtcttcgg cgacgttgcc cacatacatc gccggtttgg cggtcagcag
      841 gaacagcggt ttgagcatcg cgcgttcttc cgcgtccaaa ccgaaggaac gcacgggttt
      901 gccttcgtcc agatgcggca gcagtttttt gcacaaatcg accagctttt gcgcgtcttt
      961 gtcgcctgaa cgggcgcgtt tttcttcgcg gacgatggct ttttccacac ttgccaagtc
     1021 ggcaagcgcc aactctgtac cgatggtttc gatgtcggca atcggatcga cgcggcctgc
     1081 aacgtggacg atgttgtcgt cgtcaaagca gcgtacgacg ttaacaatcg cgtcagtctc
     1141 gcggatgttg gcaaggaact ggttgcccag accttcgcct ttgctcgcgc ctgcaaccaa
     1201 accggcgata tcgacaaatt cgacgatggc aggctgcatt ttttgcggat tgacgatttt
     1261 tgccaattca gccatacgcg ggtcgggcac ttcgacgatg ccgacgttgg gttcgatggt
     1321 acagaaagga tagtttgccg cttcgatacc cgactgggtc agcgcgttaa agagggtgga
     1381 tttgccgacg ttgggcaaac cgacgatgcc gcatttcaaa ctcatgtttt ttcctgaaaa
     1441 tagagaaatt taacggcgga ttatagcata ccgccgcccg cgttccgaaa aaatgccgtc
     1501 tgaaacggct tcagacggca tccggtttca aaaaccgttc agaacaagcc gtgaatcacg
     1561 ccgtgttcgt ccacatcgat tttctcggcg gccggaactt tcggcagacc cggcatcttc
     1621 atcatattgc cgcacagcgc gacgatgaag cccgcgccgg cggaaacggt gatgccgcgc
     1681 acggcgatgc ggaagccttc ggggcagccc aaaagtttgg cgttgtcgct caatgaatat
     1741 tgggttttcg ccatacagat cggcattttg tccaaaccca gtttttccag cgaagcgatt
     1801 tcggcggacg cttccgcgct gaaatcgaca tcttccgcgc cgtacacttt ttgggcaatc
     1861 gcacggattt tgtctttgac ggacaactcg acatcgtagg cgaaaccgaa gttattggtt
     1921 tgattttcaa tggcattgac gactttgcgc gccaaatccg cgccgcccgc gccgcctttg
     1981 ccccatactt cagtcaggga aacttcaacg ccgtgttccg cgcaggcttt ttcaatcatg
     2041 gccaactcgg catcggagtc ggacacaaag cgattgatgg caaccaccac aggcagtccg
     2101 aatacgtttt tcaggttgga aatgtgtttc agcaggttgg gcaaaccttt tgccaaggct
     2161 tcgaggtttt cttcgccgag gttggcacgc tccacgccgc cgttatattt caaagcgcgg
     2221 acggtggcca cgacaacagc cgcatcaggt ttcaaatccg ccagacggca tttgatgtcg
     2281 cagaattttt ccgcgcccaa atccgcgccg aagcctgctt cggtaacggc gtaatcggca
     2341 aggtgtttcg ccagacgggt tgcggttacg gagttgcagc cgtgggcgat gttggcgaac
     2401 gggccgccgt gtacgaaggc gggcgtgcct tcgatggttt gcaccaagtt gggcttaatc
     2461 gcatctttaa gcaatgccgc catcgcgccg tgtgccttca aatctttggc ataaacgggg
     2521 ctgccgtctt tggcgtaggc gacaaggatg ttgcccaaac gctctttcaa atcgctgatg
     2581 tctttggcaa gacagaatac cgccatcact tcggaagcaa cggtaatgtc gaaaccgtca
     2641 ggacgcatca cgccgtcaac aggtttgccc atgccgtcga tgatgttgcg caactggcgg
     2701 tcgttcatat cgaccacgcg ccgccacagc acgcgtttcg gatcgatgtt caactcgttg
     2761 ccttggtaaa tatggttgtc aagcatggcg gcgagcaggt tattcgccgc accgatggcg
     2821 tgaaaatctc cggtgaagtg caggttgatg tcttccatcg gcaaaacttg ggcatagccg
     2881 ccgcctgccg cgccgccttt cacgccgaac accggcccca gagaaggttc gcgcagggca
     2941 atcacggcat ctttgccgat gtggcgcaac gcgtccgcca aaccgatggt tacggtggtt
     3001 ttgccttcgc ccgccggagt cgggttgatg gcggtaacca aaatcagcct gccctgtttt
     3061 tgcggcagtt tgaacgcttc ggcaggattg attttcgcct tgtaatgacc gtaaggctca
     3121 atgttgtcgg cattcagacc aagcttggcg gcaatttcgc caatcgggcg catggtggag
     3181 gattgggcga tttcggcatc ggttttgaag ctcatgattt tcctttagaa atgaggaggg
     3241 acatgccgtc tgaaagcatc aggcgacaaa caggtggatt gaaaataata tcaggcatat
     3301 tataacgtta tccgcaccaa acccgcagtg aaatttttga cgctgcaaca aaaataccgt
     3361 tcatattgtt cacaatccaa ggagaaaaca tgagcagcaa cgcatggctg ttttgggcat
     3421 tggcatcggc aggcttcgcc tcattgaccg ccatcttcgc caaaatgggt ttacagggta
     3481 tagattcaga tttcgctacc ttcatccgca ccttggtcat cctcgccgct ttgttattgt
     3541 ttttaaccta caccggcaaa tggcagggcg tgaacggctt tacggggcac aactggacat
     3601 tccttatcct atccggcctt gctaccggcg catcttggct tgcctacttc aaagccctgc
     3661 aactgggtaa cgcctcgcaa gtcgcccccg tcgacaaatt cagcctggtc ttggtcgcgc
     3721 tgatggcggt ggttttcttg gacgaacgcc cgaacacgca ggaatggata ggcttggggc
     3781 tggtaacggc gggcgtgttg gtgctggcgt tgaaacgtta aaccgaatcc gccataccgc
     3841 ctgaaaccgg gttttgacaa tctgacgggc ggcggtctga acaaagttac ccaataaaat
     3901 ccgacaccgc gccgacggca aaccgccgca tccgacaggg tcggcttcag atggcatttg
     3961 ttggacggaa agccccgaac gccctatttt cacgcctcca cgaaaatatg ccggacatcc
     4021 ggtcggaacc gttcggcagc cgactcggca agccaccgtt ttatttcact tccaagcccc
     4081 tgcaagggct tgagcctctt tcagacggca taccgtgccg acatccagcc acaagcccgt
     4141 atgcttctgt ccgctcacgc ggttttgccg catttcgccc cgcaatacgg gcgcgagttt
     4201 cgccacactg cccgcttcga ttccgtcaaa catttcagga cggtaaatac ccacgccgct
     4261 gaatgtcaat ccgttgccgc cggatacttc cgactgtacg cttccgtccg gcagcaggga
     4321 aaaatcgccg tcagggttgt gcggcggatt ttccaccagc cacagatggg cggaaatatg
     4381 ttccggcagg gacgatgccg tctgaaacgc ggcggtaaaa tcgatgtcgg tcagcacgtc
     4441 gccattgacg accaaaaacg gggtatcgcc caacagcggc aatgcccgtg cgataccgcc
     4501 tgcggtttcc aagccgcctt caggttcggg cgaataggcg atgttcacgc cataagccga
     4561 gccgtcgccc aaagcatctt ctatctgccg acccagccaa gcgtggttga tgacgatttc
     4621 ggtaaacccc gcctgcttca gacggcatag gtgccaaccg attagaggct tacccgccac
     4681 atcgagcagc ggcttcggag tggtatcggt caaaggacgc atacgctcgc cgcgtcctgc
     4741 cgccagtatc atcgctttca tatatatctg tccgaatatc agtctaaaga tctaaactgc
     4801 cgtctgaaat aaagcagcgc ggggcgtttg cacccgcagt ttttgatttc gtcgagcctg
     4861 acgtaaaaca caaaatgcgt gccgatttca tgtttgccga caatatgccc gtgcaggtgc
     4921 gccaacgcgc cttctatttc aagttgtccc gttttgccgc gatgccagat gtggtaggca
     4981 aaccgctctt cgggcgacag gccggtcagc ccggcaaaat gttcggcaac atcctgatgt
     5041 tcgtccgcca gcgtattgat gcagaggctg ccgttttccg acaggatcgg aatgattcgc
     5101 gcactccggt tgatgcacag catcacggtc ggcggctcgt ccgtaaccgg cgcaaccgcc
     5161 gtcattgtaa tgccgtaacg ccctgccgca ccgtctgtcg tgatgacatg aacgcctgcc
     5221 gcacaggatg ccatcgcatc acggaacgaa gtttgaaaag ttttctgcaa atccgccatt
     5281 tttccccttt aaactgtccc ctatataaga atgctgcaca caaggcaccc cccatgtgca
     5341 gcagttctga ttcaaaaagc cgtcggtcgg acatttccgc gcgttacggc gtattacgag
     5401 ttcaacgcat cctcgatttt ggcaagttct gccaacaggt ctttaagcag cagcattttc
     5461 tcgcggccca gcacttcctc gatagcgtcg taacgctcgt ccacttcttc gccgatttcc
     5521 tcatacagct tctcgccctc ggcagtcagc ttcagaaaaa cgcggcgttg gtcgttggaa
     5581 ggtttcaggc ggacaaccaa acccgctttt tcaaggcggg tcaggatacc ggtcaggctg
     5641 gggcgcaaaa tgcacgcctg attcgccaaa tcttgaaagt ccagcgtgcc gttttccgcc
     5701 aaaagacgga taatccgcca ttgctgatcg gtaatattcg cctgattcag aataggcctg
     5761 aattgggtca tcagggcttc ccttgcctgt atcagactga tattgataga cgcatgtttt
     5821 gattgggtag gcattgttca agtctccaag ttatcgaaaa tcaaactttc aaaccgtcgg
     5881 gaaagcctgt gggcgtaaat tttgatgcaa ccgttatata acaaaacgaa catatagcaa
     5941 caatacgcta taaaccgcat cggacgactg ggtataaaag actttaattc cgataatcct
     6001 atctaaaaat attttaatag ttatatctta gtctattttc cctacaatct caacaaagta
     6061 ttacatttac tgtcgaaaga actttcttcc agaaaaaaca aaagccgccg catcagacga
     6121 gcaaggtata tgccgtttga ttctctacat agcgtaaagt ttaacaaaaa caaaagttaa
     6181 ccgaaaacat ccgcctgaaa aattcgtgcg cgcaagcccc aataactgct gattcccgtc
     6241 gtatagtgaa ccattttccc atttttgacc aaaacgacgg caggcgttgc gacaatccgc
     6301 caagaccttg ccaaaccccc gtcctcatcg ttgacagtcg gaaagcccaa gccgcgtttt
     6361 gccatatacg ccgccacttc cgccgaactg ccggaacgta ccgccacgcc gacgaccggc
     6421 acgccgtccg ccgccaaatc atcgattatc ggcgactgat aacggcacac gccgcaccag
     6481 cttccccaaa aatacaccag caccgtccta tcccggctaa actgtcccaa agtcagccgc
     6541 tgccccgaca gcagggtcaa aggccgccct gccgcgccgg caggttcgtc gggcttgcgt
     6601 acccaatcca aaaacagcga caccaataaa aacaccaatg ccgtctgaac ggcaaatttg
     6661 atgcccgaaa gcagtttctt tttcatacgc tctctcaaac ggtacgcccg cgcaaccggc
     6721 aggcaaacaa aaagccaagt ctcaaaactt ggcttccggt tatctggtgg gtcgtgagcg
     6781 attcgaacgc tcgaccaacg gattaaaagt ccgctgctct accggctgag ctaacgaccc
     6841 gataagccgt gcattataca gcaccatcct acctcgtcaa gcaaatttta caggcttaat
     6901 tgcagaccac tgtttgcacg ggatattttg acaacggatt ttcacaatcc gccgcatacc
     6961 gtgtaaaagt tcgcacaagg aaaagcaaac cgcccgaaat caatgtacac tttccgcccg
     7021 tttcccaaac tgcacacaga aacacacatt atgaacatac aaaacatccg caccctcctc
     7081 gacaccgtcg ccgttccgaa tacggcacgc acgctcggcg gcgaaaaggc cgtccgttcg
     7141 gtcgaacagc gttcagacgg catccatatc gccctgcatt tcggcttccc tgtcgcgcac
     7201 atcgccgccg ccatcgccga ttccgtacaa gaaaccctga tgcccgaaac aggcgacaca
     7261 cacatccatc tgtccatgga cacggaaatc ggcacacata aagtccaacc tggcgttacc
     7321 accatcaaag gtgtgaaaaa catcattgcc gtcgcctccg gcaaaggcgg cgtgggcaaa
     7381 tcaacaacca ccgccaacct tgccgccgca atggcgcgca tgggcgcgcg cgtcggcgta
     7441 ctcgatgccg acctttacgg cccgagccaa ccgaccatgt tgggtgtgga cgaccgcaaa
     7501 cccgatcaga aaaaccaaaa actcattccc gtcgaatctt cagacggcat acaggtcatg
     7561 tctatcggct ttctcgtcga taccgaccaa gccgtcgtct ggcgcgggcc gatggtcagc
     7621 caagccttgc agcagctgat gttccaaagc gagtgggacg aagtggacta tcttttcatc
     7681 gacctgcccc ccggtaccgg cgacatccag ctcacgctgt cccagcgcat ccccgtaacc
     7741 ggttccgtca tcgtaaccac gccgcaggac atcgccctga tagacgcgcg caaagccgtg
     7801 gatatgttcc gcaaagtcaa cattcccatt ttgggcgtat tggaaaatat gtccgtccac
     7861 atctgctcca actgcggaca cagcgaagca ctgttcggca cggacggcgg caaagatttg
     7921 gcggcacgcc tcaacgtccc cctgctcgga cagcttcccc taagcctgcc cgtgcgcgaa
     7981 gccatggacg gcggcacacc ggcgcaactg ttcgacgaac accccgccat cgccaaaatc
     8041 tacaccgatg ccgcattcca aatcgccctg agcattgccg acaaaggcaa agacttcagc
     8101 agccgcttcc ctaaaattgt cgtcgaataa agccgcgtcc gaaaccgcaa cagcaatgcc
     8161 gtcccaagcc ccgcgcctgc cgatatgccg tcattcccgc gcaggcggga atctagaacg
     8221 tggaatctaa gaaaccgtgt tgtaaaggca gaccgatgcc gtcattccca cgaaagtggg
     8281 aatctagaac gtaaaatctc aagaaaccgt tatacccgat aagtttctgc accgacaggt
     8341 ctggattccc gcctgcgcgg gaatgacggt tcgggtattt ctgacgattt aggcattccc
     8401 gacaaggtgg attttcaagg tgttgtatag ggtgtaggag gattcgtaaa aggtgagtta
     8461 tagggtgggc ttcagcccac cgattccaac gattccacca atcctacacc gttcccatag
     8521 actcaaatca acacagaaac ttatgcgccg tcattcccgc gcaggcggga atctagacct
     8581 tagaacaaca gcaatattca aagattataa aagacccgtc attcccgcgc aggcgggaat
     8641 ctagacctta gaacaacagt aatattcaaa ggttatctga aagtccggga t
//
LOCUS       DAWKOG010000079         8587 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.079, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000079 DAWKOG010000000
VERSION     DAWKOG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8587
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.079"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(239..3091)
                     /gene="gcvP"
                     /locus_tag="WJK66_001316"
     CDS             complement(239..3091)
                     /gene="gcvP"
                     /locus_tag="WJK66_001316"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEZ7579025.1"
                     /translation="MKLSELFNPNEFAARHLSFGDGAALLEVLGEKSMDDFVGNTVPQ
                     SIRMPSELDLPEALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDARVYPQTLDVMKTRAKYFGFELVVGDFAQADEGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVAADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIANRIHALASAFADALVSDGLNVVHKVFFDTVTVDFGNK
                     EKADQVFAAALESGYNLRRVNDTQVAAAFHETSACEDLVDLYRAFTGKDAFAFADDVK
                     GRLNAELLRQDDILQHPVFNRYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPEAQTVGYRELLADMENSLKAITGFDAISFQPNSGAQGE
                     YSGMLAIRRYQEAQGEAQRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIIHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHTLTDTHSASAGQT
                     AVAAAAYGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKQIKQEVLKVERGEWPKEDNPLVNAPHTASDVTGDWAHPYSREEAVFPL
                     PFVREHKFWPFVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(3122..3481)
                     /locus_tag="WJK66_001317"
     CDS             complement(3122..3481)
                     /locus_tag="WJK66_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579026.1"
                     /translation="MTDRPSLLWIKPGQAEIMEYCEPEEASDPYATYRCANLMAVLPL
                     FVVILVLLNIVFPLPAHPLAWLVPAGFMVLVGGFPLSLPLAVLLVLTCCILARCPPLS
                     RLLCYPCPNHPMSKNSA"
     gene            3429..3659
                     /locus_tag="WJK66_001318"
     CDS             3429..3659
                     /locus_tag="WJK66_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ7579027.1"
                     /translation="MISACPGFIHSRLGRSVMPSEPAGRLFQKMPYRCNFMRLYGVGF
                     ALQTAYASRSSLVSQSVHDYINNLGADKPLFI"
     gene            complement(3652..4293)
                     /locus_tag="WJK66_001319"
     CDS             complement(3652..4293)
                     /locus_tag="WJK66_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEZ7579028.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVLV
                     GIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(4283..4834)
                     /locus_tag="WJK66_001320"
     CDS             complement(4283..4834)
                     /locus_tag="WJK66_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEZ7579029.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIEDDNELFERLVLEI
                     NQAGLNWTLMLKKQQAFQTAFEGFDINTVAAFDDTDRERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRETLKYYPKWLDAI"
     gene            complement(4838..5356)
                     /locus_tag="WJK66_001321"
     CDS             complement(4838..5356)
                     /locus_tag="WJK66_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HEZ7579030.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDNTRIFVPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5356..7017)
                     /gene="pqiB"
                     /locus_tag="WJK66_001322"
     CDS             complement(5356..7017)
                     /gene="pqiB"
                     /locus_tag="WJK66_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HEZ7579031.1"
                     /translation="MTDNSPPPNRHAQARVRKNNTLLSAVWLVPLIALIAGGWLWIKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQIESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSSSPKLRPHTVYAGDTVIATQGGGLDDLQAKVVDLLDKFDKLPLDKTVAELN
                     GSLAELKSTLKSANAALSSIDKLVGKPQTQNIPNELNQTLKELRTTLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            complement(7010..8470)
                     /locus_tag="WJK66_001323"
     CDS             complement(7010..8470)
                     /locus_tag="WJK66_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEZ7579032.1"
                     /translation="MGSAVGGQPLEKIFLPDDKGLFILPNLPVLHPDWVYRLFSFIMP
                     PLIFATFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGCRADVPRLDRGEAAFCPR
                     CGYKLFRVGRHPFSAPPAYAAASLILMAFAYGMTYIEVGIPGAASVLSLPEMMRLMVF
                     QDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKRVYPALRLATRVMVRLRQAMMVD
                     VFFVSTLVAYIKLSSVAEVRFGSAFYLMFALSVMLIRTSVSVPQHWVYFQIGRLTGDN
                     AVQTASEGKTCCSRCLYFRDGAESPCGVCGAELYRRRPKSLSISSAFLTAAVILYFPA
                     NILPIMISSNPAATEVNTILNGIAYMWDEGDRLIAAVIFSASILVPVLKIAAMSVLIA
                     SARFALPTGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFHTYAARVIPGSAAVYFC
                     LVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKHD"
BASE COUNT         1924 a         2207 c         2420 g         2036 t
ORIGIN      
        1 ttgtttgtcc gtgtggaaat gacatcaaag ggtgtgaata ggcttttcat ctttgaaatc
       61 tgtttgcacg atcggatgaa taaaaggtag gatgataaaa aagctgatgt atggatactg
      121 gtttaactca tttgttttaa agttatagtc gagatacgtg tattggtgca actggtttgc
      181 cgttgatgaa aatgccgtct gaaaatgttt cagacggcat tttttaagat atcaacagtc
      241 agtcttcata attttccatc ggtgggcagc tgcacacgag attgcggtcg ccgtacacgt
      301 cgtccacgcg gttgacgaag ggccaaaact tgtgttcgcg gacaaacggc aacgggaaga
      361 cagcttcttc gcgggagtac ggatgcgccc agtcgccggt tacgtcagac gcggtatgtg
      421 gagcgttaac cagcggatta tcctctttcg gccattcgcc gcgctcgact ttcaacactt
      481 cctgtttgat ttgtttcagg gcggcgataa agcggtcgag ttcggctttg ctctcgcttt
      541 cggtcggctc gatcatcagc gtaccggcaa cggggaaaga gacggtcggg gcgtggaagc
      601 cgtagtccat caggcgtttg gcgatgtcgg tttcggtaat gccgctttcg gctttgagcg
      661 gacgcaagtc gacgatacat tcgtgcgcga cgcggccgtt tttgcctgta tacaggatcg
      721 gatagtcttc gctcagacgt ttggcgacgt agttggcgtt gagcaatgcc cagcgtgtcg
      781 cctgttccat gccttgtttg cccatcatgg tcaggtacat ccaagtaatc ggcaggatgg
      841 acgcagaacc atatgctgcg gcagccacgg cagtttgccc ggcacttgcg ctgtgggtgt
      901 cggtcaaagt gtgtcccggt gcgaacggag ccagatgggc tttcaagcca atcggaccca
      961 tgcccgggcc gccgccgccg tgagggatac agaaggtttt gtgcaggttc atgtgcaaca
     1021 cgtccgcgcc gacttcggca ggctgcatga tgccgatttg agcgttgagg ttggcgccgt
     1081 ccatataaac ctgtccgccg ttttcatgga tgatgcggca gatgtcgcgg atgccttctt
     1141 cgtacacgcc gtgggtggac ggataggtaa tcatgatggc ggacaaagcg tcgcggtgtt
     1201 gctcggcttt ggctttcaaa tcgtcgatgt tgacgttgcc gtgttcgtcg gtatcgacga
     1261 cgacgacttt caaaccgagc atggcggcgg tggcggggtt ggtgccgtgg gcggatttgg
     1321 gaatcaggca gatgttgcgc tgcgcttcgc cttgcgcttc ctgatagcgg cggatggcaa
     1381 gcataccgct gtattcgccc tgcgcgccgg agttgggctg gaaggaaatt gcgtcaaagc
     1441 cggtgatggc tttcaggctg ttttccatat cggcaagcaa ttcgcggtag ccgacggttt
     1501 gcgcttcggg agcgtaaggg tggatgtcgg agaactccgc ccaagtaatc ggcagcattt
     1561 ccgcagtcgc gttgagtttc atggtgcagc taccgagcga aatcatgctg cggttcatcg
     1621 ccaaatcgcg gtcttcgagt tttttcagat agcgcaacat ttcgtgttcg gtgtggtaac
     1681 ggttgaacac aggatgttgc agaatgtcat cttgacgcag caattcggcg ttcagacggc
     1741 ctttgacatc atcggcaaat gcgaacgcat ccttgccggt aaacgcgcgg tacaaatcaa
     1801 ccaaatcttc gcatgccgat gtttcgtgga atgcagccgc aacttgagta tcgttaacgc
     1861 ggcgcaggtt atagcccgat tccaaagcag cggcaaacac ttggtctgct ttctctttgt
     1921 tgccgaaatc gacggtaacg gtatcgaaga agaccttatg aaccacattc aggccgtctg
     1981 aaaccagcgc atcggcaaag gcagaagcca gcgcgtgaat gcggttggcg atgcgtttca
     2041 caccttcagg gccgtggtaa acggcgtaca tacccgccaa gttcgccagc aatgcctgcg
     2101 cggtacaaat attggatgtc gctttttcgc ggcggatgtg ttgctcgcgg gtggacaacg
     2161 ccatgcgcaa ggcaggtttg cccgatgcgt ctttggatac gccgatgatg cggcctggag
     2221 cggaacgttt gaactcgtct ttaaacgcga aataagcagc gtgcggacca ccgaagccca
     2281 tcggcacacc gaagcgttga gtgttgccca acgcaatgtc cgcgcccaat tcagccggag
     2341 acttcagcaa aaccaaactc atgatgtcgg cggcaacggc cacgatcgtg ccttttgctt
     2401 tcagacggcc gataacgtcc tgcaagtctt gtacatcgcc gtctttgccg acgtattgga
     2461 acagcgcacc gaagtattcg ccttcatccg cttgggcaaa atcgccgacc accagctcga
     2521 agccgaaata tttggcgcgg gttttcatca cgtccaaagt ctgcggatac acgcgcgcat
     2581 ccacaaagaa acgctcggat ttcactttgc ccacgcggtg cgccatcgcc atcgcttcgg
     2641 cggcggcagt tgcctcatcc agcaaagacg cgcctgccac agggaaaccg gtcaaatcga
     2701 tacacacttg ttggaagttc aacaaagctt ccaaacgacc ctgggcgatt tccgcctgat
     2761 acggcgtgta ggcggtatac cagcccggat tttccaatac gttgcgcaaa atcacattcg
     2821 gcacgcgggt cgggtaatag cccaagccga tataggattt gttgatcacg tttttcgacg
     2881 caatgccttt caattttgcc aacgcgtccg cttcggtcaa cgcttcgggc aaatcgagtt
     2941 cagacggcat acggatgctt tgcggcacgg tattgccgac aaaatcgtcc atgcttttct
     3001 cgccgagcac ttccaaaagt gcagccccgt cgccaaagct caaatggcgc gcggcaaatt
     3061 cgttcgggtt gaacagttcg gacagtttca tttctactcc ttcgggtttg tagggctgcc
     3121 atcaggcaga atttttagac atcggatgat tcgggcaagg gtagcacaaa agacgggata
     3181 atggcggaca acgcgccaga atgcagcagg tcaggacaag cagcaccgca agcggcagcg
     3241 ataagggaaa gccgccgacc aaaaccatga aacctgcagg caccagccaa gctaagggat
     3301 gcgccggaag cggaaaaaca atattgagca gaaccaaaat cacgacaaac agcggcagca
     3361 ccgccatcag gttggcacac ctgtatgttg cgtacgggtc ggacgcttct tcaggttcgc
     3421 aatattccat gatttcagcc tgtccgggct ttatccacag caggctcgga cggtcggtca
     3481 tgccgtctga accggcaggc cgtctttttc aaaaaatgcc ataccgctgc aacttcatgc
     3541 ggctgtatgg cgtaggtttt gcacttcaga cggcatatgc gtcccgaagc agtttggtgt
     3601 cccagtcggt tcatgattat ataaacaact tgggtgcgga taaacctttg tttatctaaa
     3661 cccctgtttt ctcaaaaatt gattcaaatc ttcggcaaat ttccgatagc ctttgccgtt
     3721 ggcgtggatt tggtcggatt tcagattatt atcgcccaaa atttccgccc acgcgccgcc
     3781 gaacagcgga atgccgtatt cctcggacaa atcctcatac agcggatgat cgctcaaatg
     3841 cccgaacaac gcgcccaagg taatgtgcgg cacgccgacg aggacggcgg ggatgttttc
     3901 cttctgcacg gtttcgatga ttttcgcgat attggcgcgg gtctgctcct tgggaacttt
     3961 gcgcagaaag tcgttgccgc ctatgccgac aagcacaagc ttgggtttgc gtgccaacag
     4021 cgcgggcagg cgcgacaggg cttgggcgga tgtatcgccc gatacgccgc cgttgacaat
     4081 attccaaccc gtcagttttt gcagttgcgc ggggtaggat tcgccagggt ttgcgccgta
     4141 gccgaaggta agcgaatcgc ccaaggcaag tacggtgctt ccttcgggaa ttttggcgtg
     4201 ggttcgggcg gattttctgc cgcaggcggt aagcagcaac gcgcctgcgc cgaggaggaa
     4261 ggttcttctg ttcattggtt tttcagatgg catcgagcca tttcgggtag tatttcaggg
     4321 tttcacggta aaccggacag ctttcggcgt gcgcgccttt gaggtagccg gtactcatca
     4381 gaaattcgcc gacgatttcg ccgccgacga atttgaaatg ttttttaaag agtttaaccc
     4441 attcgtcttt gcttcgcgga tggtgcgcgt cgagccagtt cttgaacgag ccgtattctt
     4501 gttgcaacgc ttggatttgc cgtgcattga aaatggcggc atcgattttc aggcggttgc
     4561 ggacgatgcc cgcgtcggca agcaggcgtt cgcggtcggt gtcgtcgaag gcggcaacgg
     4621 tattgatgtc gaacccttca aatgctgtct gaaacgcctg ctgcttcttc agcatcagcg
     4681 tccagtttaa tcctgcctga ttgatttcca acaccagccg ctcaaacaat tcattgtcgt
     4741 cctcaatcgg aaaaccgtat tgcgtgtcgt ggtaatgttt gttcgggtta tcggtgtttt
     4801 cgggaagtga ggcggcaaat tcgcaatagt tcacggttta ctcgaccatc tgttgcgccg
     4861 cctgtttcag tccctgttcg agtgcggcgg tcatcgcggc gtagccgtca ccctgctgtt
     4921 cggtttcgat atggaagggt ctgttcgtac cgtcgggtag gacggcgtag ccgctgatga
     4981 gggttttgcc cgtgtagctg ccttggaatg cgtcgatata gaccgtccat ttttcggtac
     5041 tgccgctgcg tgaggcagga acaaagatgc gtgtgttgtc caaacggttg aatgcattgc
     5101 tcaacgccgc ttcgagcata tcgtccaagg tgtctgccca aacgtggttt tgtgcggtgt
     5161 tgaggcggta ggggtcggtt tgatagacca gtccgccgcg tttgagcggt tcggcaagac
     5221 ggacttcgac ggcggtttcg ccgccttgtg ttgcaggacg gatgtagcgg ctgtcgggca
     5281 acacgaaata ttgtgtgctt tgcacagtac cgcaggcggc aagcgtcagg gcggcggcaa
     5341 tcgggaagag gcgcattatc ggcttccttt cgggatgggg tctttgctgc tgctgttgaa
     5401 aatcagcgcg ttgggttttt ctttcaaagt gttaatgacg ggttgaacgt cttttaaggt
     5461 tttgtccaaa ctttgcaacg tattttgtac gtcgccgtag ataggcgatt gaggcgatac
     5521 gccttgcagg gttgtgcgca actctttcag ggtttggttc agttcgttcg gaatgttttg
     5581 tgtctgcggt ttgccgacca gtttgtcgat ggagcttagg gcggcattgg cagatttgag
     5641 tgtggatttg agctcggcaa gcgaaccgtt caattcggca accgtcttat ctaaaggcag
     5701 tttgtcgaac ttgtccagca aatccaccac tttcgcctgc aaatcgtcca aaccgccgcc
     5761 ttgtgtagcg atgacggtat cgcctgcata aacggtatgc ggtcgcagct tgggcgaaga
     5821 cgaaggctga tcgttcaact caatcatttt gctgccggtc agcaggttgt tgctggaaat
     5881 ggtggcggtc aggcctttgt ttaaggccgt ctgaaattgt tgtttccaat gttctttgct
     5941 ttgctcgtcg gcattgattt ccaaacggga aggctcgatg cggatgcgta cgggaatcca
     6001 accgttttca aacagatgca ggctgtcgtt gcggtcgaaa taagggacat cggaaaccac
     6061 gccgacattc agccctttgt actcgacggg cgaaccgacg gtcagtccgc gcacggattg
     6121 tttgaaaaac gcggtgtaat acagcgagcg gtcgtcaggc aggttggcga cttcgctgcg
     6181 gctgtcgtaa agcgtgaagc tgtcttcgct tttgacgttt ttactgtttt tggttttcgg
     6241 cgaatcaaat gaaatcgcgc ccgacagcag ggcaggcaga ggggcggaat tgagtttgat
     6301 gccgctgcct gtggtttcga tattaatgcc gctttccagc caaaaacggc tggcggaatg
     6361 aatcagtttg tcgttggggc tttggatgaa gatggtgtaa tgcacgcttt ggtcggacgg
     6421 gtcgaaatgc gcgctttcga tttgcccgac cataaagttt tcatacaaaa cagggctgtt
     6481 gacgttgagg atgcggtcgt ttttcccaat caaattcaag cgcagtccgc tttgcccgat
     6541 ggcggtaacg ggcggaatgt cctgcacttg gaacacgtct tttgcctcgt cgcttttgcc
     6601 gggtgtaaaa gcgatgtacg aacccgaaag cagcgtaccc aaaccggtta cgccgctttg
     6661 gtcgatacgc ggcttgacca cccaaaactg tgtgtcgctg cggatgaggc cggatacgtc
     6721 cgcattgagt tgggcggtta cttccacacc tttctggtcg tcgcgcagtt tgattcgggt
     6781 aacgcgtccg acatcgatgc tcaatacctt aatgaccgta ttgttgacct caatgccttc
     6841 cgcgctgtcc atcaagagcg taaccacagg ccccctgttg cggatttcct taatccacag
     6901 ccagccaccg gcaatcagcg caatcagcgg gaccagccag acggcggaga gcaaggtgtt
     6961 gtttttgcgg acgcgtgctt gggcgtgtct gtttggagga gggctgttgt cagtcatgtt
     7021 tttccgtttc attgaaagca atgccgtctg aagcgcgttt gtcccaaagc aggcgcgggt
     7081 cgaaataata ggcggacagc atcgtcagaa tcacgaccag gcagaaatag actgccgcac
     7141 tgcccggaat gacgcgcgcg gcataagtgt ggaacgaaca catcaaaata ataatcacaa
     7201 aaatatcaat catcgaccag cggccgaccg cttcggtgat gcggtagagg tgcgacaatt
     7261 tctttgcacc cgttggcaaa gcgaagcggg cggacgcaat caaaaccgac attgccgcaa
     7321 tcttcagtac cggcaccaaa atactcgcgc tgaaaataac cgccgcaatc agcctgtcgc
     7381 cctcgtccca catataagcg atgccgttaa ggatggtatt gacctccgtg gcggcaggat
     7441 tggacgaaat cataatcggc aggatattgg cagggaaata caaaataacc gccgccgtca
     7501 gaaacgccga cgaaatactc agacttttcg gccgtcggcg gtacagttcc gcaccgcaca
     7561 cgccgcaggg ggattcggca ccgtctcgga aatacaggca gcggctgcaa caggttttgc
     7621 cttctgatgc cgtctgaacc gcattatccc ccgtcagccg cccgatttga aaatacaccc
     7681 aatgctgggg aaccgatacc gaagtccgaa tcagcataac cgacagcgcg aacatcagat
     7741 aaaacgccga cccgaagcga acctctgcca cagacgagag cttgatatac gccaccagcg
     7801 tggaaacaaa aaacacatcc accatcatcg cctgcctcaa gcgcaccatc acacgcgttg
     7861 ccaaacgcag cgcaggatac acccgtttcc gtatcagcgc ggcatagaca tacaggcaca
     7921 gcagcagaaa cagaaccggc gcgccgaagg tcagcacaaa catcacttcg gccaaaaaac
     7981 cataatcctg aaacaccatc aggcgcatca tctcgggcag cgaaaggacg gatgccgcac
     8041 ccggtatccc gacctcgata tacgtcatac cgtaagcaaa cgccatcaaa atcagcgaag
     8101 ccgccgcata agcgggcggg gcggaaaaag gatgcctgcc caccctgaag agcttatagc
     8161 cgcagcgcgg gcagaacgcc gcctcccccc tgtccaaccg aggcacatcc gcgcggcagc
     8221 cgcattccgg gcaatctacc gtatgcggcg gtagggcttc gtcgcggcag ttgccgcgaa
     8281 ggctgtgggg gatattttcg gcaaacggct tcatcgggaa agtggcaaat ataagtggcg
     8341 gcattataaa ggaaaaaagg cgatacaccc aatcaggatg caaaaccggc aggttcggca
     8401 gtatgaacaa acccttgtcg tccggcaaaa atattttttc gagcggctgt ccgccgactg
     8461 ccgaacccat ttgataaaat cccgattctg taaagaatag taaaatacct gcttaaaaac
     8521 atcacgcaaa tgatattttt tccaaaaata tgtgataata aatttcgttt atgattcttt
     8581 attattt
//
LOCUS       DAWKOG010000080         8564 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.080, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000080 DAWKOG010000000
VERSION     DAWKOG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8564
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.080"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(77..595)
                     /locus_tag="WJK66_001324"
     CDS             complement(77..595)
                     /locus_tag="WJK66_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEZ7579033.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
     gene            complement(615..2000)
                     /locus_tag="WJK66_001325"
     CDS             complement(615..2000)
                     /locus_tag="WJK66_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEZ7579034.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            2239..3780
                     /locus_tag="WJK66_001326"
     CDS             2239..3780
                     /locus_tag="WJK66_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEZ7579035.1"
                     /translation="MDGRRWAVWGAFALLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAGASALVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGGYVLL
                     AFAAAVLSVCCLFPLLAIVVKAWSAGESWRVLMESETRQAVWNTLRFSAAAVFAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPGWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITLPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFAVCIFLLLDGGEGGK
                     RTETL"
     gene            3809..4408
                     /locus_tag="WJK66_001327"
     CDS             3809..4408
                     /locus_tag="WJK66_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEZ7579036.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            4405..5010
                     /gene="nadD"
                     /locus_tag="WJK66_001328"
     CDS             4405..5010
                     /gene="nadD"
                     /locus_tag="WJK66_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEZ7579037.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPTGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVREGATYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLYQTPRELHAWLGKSLQDGSVRIL
                     SAPMHNVSSTEIRRNLAGQGVSDGIPPAAARYIREHGLYEK"
     gene            5069..5455
                     /gene="rsfS"
                     /locus_tag="WJK66_001329"
     CDS             5069..5455
                     /gene="rsfS"
                     /locus_tag="WJK66_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEZ7579038.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            5509..5979
                     /gene="rlmH"
                     /locus_tag="WJK66_001330"
     CDS             5509..5979
                     /gene="rlmH"
                     /locus_tag="WJK66_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEZ7579039.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKSWQQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVLLTEQLYRAASILHNHPYHRE"
     gene            6054..6320
                     /locus_tag="WJK66_001331"
     CDS             6054..6320
                     /locus_tag="WJK66_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEZ7579040.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            6477..7160
                     /locus_tag="WJK66_001332"
     CDS             6477..7160
                     /locus_tag="WJK66_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEZ7579041.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVPLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            7284..7796
                     /gene="coaD"
                     /locus_tag="WJK66_001333"
     CDS             7284..7796
                     /gene="coaD"
                     /locus_tag="WJK66_001333"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEZ7579042.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETIHRYVPQAVYE
                     KILAEHQHEN"
     gene            7828..8481
                     /locus_tag="WJK66_001334"
     CDS             7828..8481
                     /locus_tag="WJK66_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HEZ7579043.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNRESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRRGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWKIQFAYQNRQIEIVAPLKNEWQTENISHALEEFYMAKAG"
BASE COUNT         1861 a         2271 c         2508 g         1924 t
ORIGIN      
        1 aatgtaatcc attctgttcc cgatgtttat gccgtctgaa acccatcctg tgtcgggctt
       61 cagacggcat attgcttcaa agcaggtttt ccgaggcaac ccagttcaga atatcggctt
      121 cgaccgcttc gggcgtgccg gggttggcga ttttgacccc gtattcgcct tcgcccagtt
      181 cctcgaggat ttccagttgc gaatcgagca tccctgcttt catgtaatgt cctttgcgcg
      241 acatcatgcg ctcgaggttg atgtcttgcg gcggactgag gtggatgaag gcagctttgc
      301 cttcggctcc gcgcagaatg tcgcggtagc cgcgtttgag ggcggaacag gttacgatgg
      361 tgtggttcgc accgttttgc gcctgttgcg tcatccagtc gcgcagattg cccaaccacg
      421 gatagcggtc ttcatcggtc agcggaatac ccgcgcccat cttgtcgcgg ttggcttggg
      481 tgtggaactc gtcgccttcg gcatagggac attgaccgag gtgtttctgc agggacagcg
      541 cggcggtggt cttgccgcag ccgcatacgc ccataacgac aaaatgcgta gtcatttact
      601 atcctttccg tctgtcagac gatggcgaac agcagtgcgg acaaggcaaa gccgatgagt
      661 gcgatgaggg tttggttgac cgtccaggtt ttcagcgtgg tcggtacgtc catgtccaag
      721 agacggccga ccagccagaa gccggagtcg ttgaagtggc tgcaaccgac cgaacctgcc
      781 gccgttgcca atacgataca ggcgagctgc cagtcggtaa agccggcggc ggcaacggca
      841 ggagccatca gcgcggcggc ggtggtcagg gcgacggttg ccgaaccttg cgcgatacgc
      901 agtgccaagg cgacaaggaa acagcccaaa aggacgggaa tgcccaaatc cgccatgctg
      961 tcggcgagtg ccttgccgat gccggaagcg cgcaaaacgc cgccgaacat accgcccgcg
     1021 ccggtaatca gaatcacgga acagacgggg gcgagtgcgc cgtccacggt tttttccaac
     1081 gcgctgccgc tttcgccgcg tttgcgtccc aagacaaaca gtgcgaccaa tacggaaatc
     1141 agaagggcga tcggtgtcga accgattatt tttgccgtct gaacccaggt ttcgtccgca
     1201 cttacgagtt tttcgctgat gagggccgat acgccggtat tcaggaaaat cagcagcatg
     1261 ggaatcagca tgatggcgac gaccgttcct gctttggcag gttctttcgg caggtcgttg
     1321 tcttgcgtgc cgccgctgag cagttcggga acgggaacat ggatggtgcg ccccaacact
     1381 ttgccgagca tatagccgct gaaataccat gtgatgaagg cggtcggcag acccaaaatc
     1441 aaaacttggc cgatgttcgc gccgtaaaat tcggaagcgg caatcgggcc cggatggggc
     1501 ggcaggaaga cgtgcatgac ggaaaatgcg ccgatggagg caagcgcgaa gggcagtacg
     1561 tcctgtttca tgcgccgtgc ggtggcgaac acgatgggca gcatgacgat tagtccggca
     1621 tcgaagaaaa tcgggaagcc gaaaatcagc gaggcaacgc ccagcgcgaa cggtgcgcgt
     1681 ttttcgccga acatccggat cagcgcgtcc gccagcgact gtgcgccgcc ggatgtttcg
     1741 accagacgtc cgagcatcgc gcccaggccg accagaagcg ccacgccgcc gagcgtgccg
     1801 ccgaagtttt tgaccagtac gtcgttgaca atgctgcctg tgggcaaacc ggttgccaaa
     1861 gccgtcagca ggctgacgat gaccagtgtc agcagcgcgt ggatgcggaa tttgacgatt
     1921 aaaatcagaa tgaggatgat tgccgccgcc gaaatgccca acagggtttg cgcggacagc
     1981 gtctgtgtcc agccgtccat ttgtaaagcc tttctctgta ataaaagtac aaaattgtca
     2041 ggttttttta agttgtgcga aaacgatagc acaaatcggg cggtaagttg tttgtctgaa
     2101 gttatattcc tgtttatttg aacgatttat ttcagacggc gggaggaagg cggatacggt
     2161 atgtagtcgg attgaaatat acggtatgtg gggcttttgg gtacaatcgc aacatcagtg
     2221 tttcaagtgg ggaaggcgat ggatggacgg cgttgggcgg tatggggtgc ttttgccctg
     2281 ctgccttcgg cttttttggc ggtaatggtc gttgcgcctt tgtgggcggt ggcggcgtat
     2341 gacggtttgg cgtggcgcgc ggtgctgtcg gatgcctata tgctcaaacg tttggcgtgg
     2401 acggtatttc aggcagcggc aacctgtgtg ctggtgctgc ctttgggcgt gcctgtcgcg
     2461 tgggtgctgg cgcggctggc gtttccgggg cgggctttgg tgctgcgcct gctgatgctg
     2521 ccttttgtga tgcccacgtt ggtggcgggc gtgggcgtgc tggctctgtt cggggcggac
     2581 ggcctgttgt ggcgcggacg gcaggatacg ccgtatctgt tgttgtacgg caatgtgttt
     2641 ttcaaccttc ctgtgttggt cagggcggcg tatcaggggt ttgtgcaagt gcctgcggca
     2701 cggcttcaga cggcacggac gttgggcgcg ggggcgtggc ggcggttttg ggacattgaa
     2761 atgcccgttt tgcgcccgtg gcttgccggc ggcgtgtgcc ttgtcttcct gtattgtttt
     2821 tcggggttcg ggctggcatt gctgctgggc ggcagccgtt atgccacggt cgaagtggaa
     2881 atttaccagt tggtcatgtt cgaactcgat atggcggggg cttcggcact ggtgtggctg
     2941 gtgttggggg taacggcggc ggcagggttg ctgtatgcgt ggttcggcag gcgcgcggtt
     3001 tcggataagg cggtttcacc cgtgatgccg tcgccgccgc agtcggtcgg gggatatgtg
     3061 ctgctggcgt ttgcggcggc ggtgttgtct gtgtgctgcc tgtttccttt gttggcaatt
     3121 gttgtgaaag cgtggtcggc cggcgaatcg tggcgtgtgt taatggaaag tgaaacgagg
     3181 caggcagtgt ggaatacttt gcgcttctcg gcggcggcgg tgtttgcggc ggcggttttg
     3241 ggtgtggtgt atgcggcggc ggcgcggcgg tcagcgtgga tgcgcgggct gatgtttttg
     3301 ccgtttatgg tgtcgccggt ttgtgtttcg gcgggcgtgc tgctgcttta tccggggtgg
     3361 acggcttcgt tgcccttgct gctggcgatg tatgcgctgc tggcgtatcc gtttgtggca
     3421 aaagatgttt tatcagcctg ggatgcactg ccgccggatt acggcagggc ggcggcgggt
     3481 ttgggtgcaa acggctttca gacggcatgc cgtattacac tccccctctt gaaaccggcg
     3541 ttgcggcgcg gtctgacttt ggcggcggcg acgtgtgtgg gcgaatttgc ggcgacattg
     3601 ttcctgtcgc gtccggaatg gcagacgttg acgactttga tttatgccta tttggggcgt
     3661 gcgggtgagg ataattatgc gcgggcaatg gtgttgacat tgctgttgtc ggcatttgcg
     3721 gtgtgcattt tcctgctgtt ggacggcggc gaaggcggaa aacggacgga aacgttataa
     3781 tgcgaaccct ttttcagagg acggcaaaat gagcgagttg gataacatcc ttgcccacaa
     3841 ccggcagttt gtcgaatcgg gcgaatatga aaaatacttt accgacaaat accccgaacg
     3901 cgggctggcg gttttgtcct gtatggatgc gcggattatc gggctgctgc ccgacgcgtt
     3961 gggtttgaaa aacggcgatg ccaagctgat taaaaatgcc ggcgcgctgg ttacgcaccc
     4021 gtggggttcg gtgatgcgga gccttttggt tgccgtgttt gaattgaagg tcagagagat
     4081 tatggtcatc gcccatcacg attgcggtat gcaggggctg aatgccgaag aattcctcgg
     4141 gcgcgtccgg gaaagccgga ttcccgaaga ccgtatcgaa accctgcgtt atgccggtat
     4201 cgacctcgac ggctggctga ccggtttcga caacgtcgaa gacagcgtgc gccacacggt
     4261 ggaccttatc cgtaaccatc cgctgatgcc gcgccatatt gccgttcacg gattggtcat
     4321 ccatcccgtt accggcaaac tgacgctggt tgtggacggc agtgtttcag acggcatgga
     4381 cttatcggaa ggaatggaaa catcatgaag aaaatcggac tgttcggcgg cactttcgac
     4441 ccgatacaca acgggcatct ccacatcgcc cgcgcctttg ccgacgaaat cggcttggac
     4501 gcggttgttt tcctgccgac aggcggcccg tatcacaaag acgcagcctc cgcttccgcc
     4561 gccgaccgcc ttgccatggt cgaactggcg acggcagaag acgcgcgttt tgccgtcagc
     4621 gattgcgaca tcgtccgaga aggtgcaacc tatacttttg ataccgtcca aatcttccgc
     4681 cagcagttcc catccgcgca actctggtgg ctgatgggca gcgacagcct gatgaagctg
     4741 cacacatgga aaaaatggca gatgctcgtg cgcgaaacca atatcgccgt cgccatgcgg
     4801 cagggcgaca gtctgtacca aaccccgcgc gaactgcacg cgtggctggg caagtccctt
     4861 caggacggca gcgtccgcat cttgtccgcc ccgatgcata atgtgtcgtc aacggaaatc
     4921 cgccgcaacc ttgccggcca aggcgtttca gacggcatcc cgcctgccgc cgcacgctac
     4981 atccgcgaac acggtttgta tgaaaaataa agtcaaatca gtaagaaacg gctataatgc
     5041 cgtctgaaaa catcccgtta ggaacataat gaacgaacaa gaactgcaag acctgcaaaa
     5101 aatggtcggg gtcgccgtca acgccctcga agacatcaaa gccaaagaca tttccgttct
     5161 cgaaacgcaa gacaaaactt cgctgtttgc cagaatgatt atcgccagcg gcgacagtac
     5221 gcgccaagtc aaagcactgg caaacaacgt tgccgtcgat ttgaaagaag ccggttttga
     5281 aatcctcagc accgaaggcg acagcggcga atggacgctc gttgatgcag gagacctcgt
     5341 cgtccacgtt atgctccccg ccgtgcgcga cttctatgat atcgacaccc tttggggcgg
     5401 cgagaaaccg agttttcacg ccggaatgca gaagccgtgg cacgcggcag actgattccc
     5461 gatgccgtct gaatgttcag acggcatttt cctgtaaggg agaaagcatt gaacatcacc
     5521 gttttggcag tcggcaccaa aatgccgcgc tgggttgatg aggccgtcgc cgaatacgcc
     5581 aaacgcttcg gacgcgacgt cgcctacgca ctcaaagaaa tcaaacccga aaaacgcggc
     5641 gcgggcgtga atgccgccca aggtatggcg gcggaagaaa aacgcatcct tgaagccatt
     5701 ccgcaaggcg cgttcctcgt cgttcttgac gaacgcggca aagcaccgac ctccgtcgag
     5761 ctggcggaac acctcaaaag ctggcagcaa aacggcgaac acgtctgctt cgtcatcggc
     5821 ggcgcggacg gtatgaccga ccgcctcaaa cagcaggccc gcatgatgat gcgcctgtcc
     5881 agcctgaccc tgccgcacgg gatggtgcgc gtccttctga ccgaacaact ctaccgcgcc
     5941 gcctccatcc tgcacaacca cccctaccat cgggaataag aaggcttttg tccgcactcc
     6001 caatcggtta aaatcccccc gttatttctg aacacacagc aaaggaatcc gctatggcac
     6061 gcatggtatt ctgcgtcaag ctcaacaaag aagccgaagg catgaaattt ccgccgcttc
     6121 ccaacgaatt gggcaagcgc atttttgaaa acgtatcgca agaagcgtgg gcggcgtgga
     6181 cgcgccacca aacgatgctg attaacgaaa accgtttaag cctcgccgat ccgcgcgcgc
     6241 gcgaatacct ggctcagcag atggagcagt atttcttcgg cgacggcgcg gatgccgttc
     6301 agggatacgt tccgcaataa cggttttccg ttttgaacac aggctgtccg aaactgcttc
     6361 agacggcctt taaaatacgc cggacaactt tatcttcacg caaccggaca aattttgaca
     6421 ttgggcaatg ttaaaatccc ccattattcc caaccgcgct ttcaggagca gatgatatgc
     6481 aacacgacgt ttacgactac accgcgcata cggtttctaa aaacaccgtc ctgcagaaaa
     6541 cctaccgcct gctcggattt tcattcattc cggctgccgc aggcgcggca cttgccgcca
     6601 atgccggttt caatttttac gccgccttcg gttcgcgctg gatagggttt gccgtcgtgt
     6661 tggcgttttt ctacggtatg atccacttca tcgagaaaaa ccgttacagc aataccggcg
     6721 ttcccctgct gatggtattc acattcggta tgggcgtatt gatcggcccc gtgctgcaat
     6781 acgcgctcca tattgccgac ggtgcgaaaa tcgtcggcat tgccgccgcg atgaccgccg
     6841 ccgtcttttt aacgatgtcc gccttggcgc gccgaacccg gctcgatatg aacgcgctcg
     6901 gacgcttcct gaccgtaggt gcggtcattc tgatggtcgc cgtggttgcc aatctgtttt
     6961 tgggtattcc cgcactcgcc ctgaccattt ccgccggttt tgtcttgttc agttccttaa
     7021 tgattatgtg gcaggtacgc accgtcatcg acggcggcga agacagccat atcagcgcgg
     7081 cactgacact gtttatctcg ctttacaaca tcttcagcag cctgctgaac atcctgttgt
     7141 ctttaaacgg cgaggattga tgtcggtaaa acgccgtccg actatgccgt ctgaaaatgc
     7201 ttcagacggc attttacttt gggcatacaa tacggatgca cacgcaccaa cacacagccg
     7261 gctatgaagc cgggaggaat caaatgttac cgaatacacc gcgccgcgcc gtttatgccg
     7321 gcagtttcga tccgcccaca ttggggcatc tgtggatgat acggcaggcg caatctatgt
     7381 ttgacgaact catcgtcgcc atcggcatta accccgacaa acgcagcacc tataccgtcg
     7441 ctgaaaggca ggatatgttg tgcgatatta ctaaaatgtt tcccaacgtc agaaccgatg
     7501 tatttgaaaa ccgatttctg gtgcattatg cccgtgaggt agatgcagga ttcatcgtgc
     7561 gcggcatccg ttctgcttcg gattacgaat acgaacgttc catgcgccac atcaacagcg
     7621 acctcgcccc cgaaatatcc accgtattcc tcatgccgcc gcgcgaaatc gccgaagtgt
     7681 cgtccactat ggtcaaagga ctggtcgggc cggaaggctg gacggaaacg atccaccgct
     7741 acgtgccgca agctgtgtac gaaaaaatcc ttgccgaaca tcaacacgaa aattgaaccc
     7801 ttctattttt gcaaggcata tatggatatg ttggaaattt tgttccgcca tcaagacttc
     7861 gtcgccatca acaaaccggg cggcatatcc gtccaccaag acagcggcga gaccggactg
     7921 gcccgaacac tcgccataca gttgggcgta gagcgtgtgt ggctgctgca ccgattggac
     7981 aaacagacca gcggcatttt actgtttgcc ctcaacagag aaagcgcgtc cgcccttagc
     8041 ggacagtttg ccggcaaaag catcaaaaaa acctatttgg cactatccga ccgcaaaccg
     8101 tccaaaaagc agggttggat taaaggcgga atggaaaaat ccagacgcgg aatgtggaag
     8161 ttgacgcgca atacagaaaa tatcgccgtt acccgattcc acagcatcag tatcgccgaa
     8221 aaactgcggc tgttcatcct ggaaccgcat acgggcaaaa cgcaccaatt gagagtggcg
     8281 atgaaaagtt tgggcagtcc gattctgggc gacagcttat acggcggaac agaatccgaa
     8341 accatgtttc tgtatgcatg gaaaatacaa tttgcctacc aaaaccgaca aatcgaaatt
     8401 gttgcaccct taaaaaacga atggcagact gaaaatatct ctcatgcgct ggaagaattt
     8461 tatatggcaa aagcaggtta ataaatataa attaatttat aaacaatatg ataaatattt
     8521 ttataaaaat ttttcaagaa aacggttgac tgcaaatctc ttca
//
LOCUS       DAWKOG010000081         8466 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.081, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000081 DAWKOG010000000
VERSION     DAWKOG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8466
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.081"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            252..2363
                     /locus_tag="WJK66_001335"
                     /pseudo
     CDS             252..2363
                     /locus_tag="WJK66_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            2605..4068
                     /locus_tag="WJK66_001336"
     CDS             2605..4068
                     /locus_tag="WJK66_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ7579044.1"
                     /translation="MVIFYFCGKTFMPARNRWILLPLLASAAYAEETPREPDLRSRPE
                     FRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGIR
                     VILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAAA
                     LFEDRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNIN
                     QAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKFN
                     DMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLSS
                     AEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRYG
                     LRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRALH
                     FKGITPRLTLSHRETWSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(4269..5237)
                     /locus_tag="WJK66_001337"
     CDS             complement(4269..5237)
                     /locus_tag="WJK66_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579045.1"
                     /translation="MKLTLAIPSLNLDEDETRIPLCLPAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSEAVALATPVWQKMGMHQANVLTAEYLNVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPCAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWDSLHGSSQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAYLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     IYEAVRTGKIKRLDITTDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            5315..6607
                     /locus_tag="WJK66_001338"
     CDS             5315..6607
                     /locus_tag="WJK66_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEZ7579046.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTERTERVRPQRV
                     EQKLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLIYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            complement(7110..7796)
                     /locus_tag="WJK66_001339"
     CDS             complement(7110..7796)
                     /locus_tag="WJK66_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HEZ7579047.1"
                     /translation="MYAFTAAQQQKALFWLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFNKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDEFMAANWQGIAFVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
     gene            complement(7871..8344)
                     /gene="queF"
                     /locus_tag="WJK66_001340"
     CDS             complement(7871..8344)
                     /gene="queF"
                     /locus_tag="WJK66_001340"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEZ7579048.1"
                     /translation="MSRNTEELQGISLLGNQKTRYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIYIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFEALARKRLFEHDAQ"
     regulatory      complement(8348..8392)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT         2039 a         2088 c         2505 g         1834 t
ORIGIN      
        1 tcccgttatt tccacaaaac agaaaatcaa aaacagtaac ctgaaatccc gtcattcccg
       61 cgcaggcggg aatccagacc tttaaacccc gaccatcctt gataaattct tgcggcatta
      121 aaattctaga ttcccgcttt cgcgggaatg acggcggagg gttttttgct tttcctgatt
      181 tttcattgcg atgtagtata atgtagtata taatcattat aattttaaca cttgacaaag
      241 gaaaatttct catgacactg aaagcaagca agcaagcaag caagcaagca agcaagcaag
      301 caagcaagca agcaagcggt cgggttaatc tattaacatt atctgtttta tcgctgtttt
      361 gcacgccata tgtttgaggt tcggatgcgt acgatcccgt caaagaagcc gagattaaaa
      421 acaaatttat tttagaagcg gcggaagaca gaaattccca cgtttggcgc ggcccgtgca
      481 gcatatcttt tgattgcttc ggtatgttca gagctcagct tggttcaaat actcgttcta
      541 ccaaaatcgg cgacgatgcc gatttttcat tttcagacaa gccgaaaccc ggcacttccc
      601 attatttttc cagcggtaaa accgatcaaa attcatccga atatgggtat gacgaaatca
      661 atatccaagg taaaaattac aatagcggca tcctcgccgt cgataatatg cccgttgtca
      721 aaaaatatat tacagagaag tatggggctg atttaaagca ggcggttaaa agtcaattac
      781 aggatttata caaaacaaga ccggaagctt gggcagaaaa taaaaaacgg actgaggagg
      841 cgtatatagc acagtttgga acaaaattta gtacgctcaa acagacgatg cccgatttaa
      901 ttaataaatt ggtagaagat tccgtactca ctcctcatag taatacatca cagactagtc
      961 tcaacaacat cttcaataaa aaattacacg tcaaaatcga aaacaaatcc cacgtcgccg
     1021 gacaggtgtt ggaactgacc aagatgacgc tgaaagattc cctttgggaa ccgcgccgcc
     1081 attccgacat ccatatgctg gaaacttccg ataatgcccg catccgcctg aacacgaaag
     1141 atgaaaaact gaccgtccat aaagcgtatc agggcggtgc ggatttcctg ttcggctacg
     1201 acgtgcggga gtcggacaaa cccgccctga cctttgaaga aaaagtcagc ggacaatccg
     1261 gcgtggtttt ggaacgccgg ccggaaaatc tgaaaacgct cgacgggcgc aaactgattg
     1321 cggcggaaaa ggcagactct aattcgtttg cgtttaaaca aaattaccgg cagggactgt
     1381 acgaattatt gctcaagcaa tgcgaaggcg gattttgctt gggcgtgcag cgtttggcta
     1441 tccccgaggc ggaagcggtt ttatatgccc aacaggctta tgcggcaaat actttgtttg
     1501 ggctgcgtgc cgccgacagg ggcgacgacg tgtatgccgc cgatccgtcc cgtcaaaaat
     1561 tgtggctgcg cttcatcggc ggtcggtcgc atcaaaatat acggggcggc gcggctgcgg
     1621 acgggcggcg caaaggcgtg caaatcggcg gcgaggtgtt tgtacggcaa aatgaaggca
     1681 gccgactggc aatcggcgtg atgggcggca gggccggcca gcacgcatca gtcaacggca
     1741 aaggcggtgc ggcaggcagt tatttgcatg gttatggcgg gggtgtttat gctgcgtggc
     1801 atcagttgcg cgataaacaa acgggtgcgt atttggacgg ctggttgcaa taccaacgtt
     1861 tcaaacatcg catcaatgat gaaaaccgtg cggaacgcta caaaaccaaa ggttggacgg
     1921 cttctgtcga aggcggctac aacgcgcttg tggcggaagg cgttgtcgga aaaggcaata
     1981 atgtgcggtt ttacctgcaa ccgcaggctc agtttaccta cttgggcgta aacggcggct
     2041 ttaccgacag cgaggggacg gcggtcggac tgctcggcag cggtcagtgg caaagccgcg
     2101 ccggcattcg ggcaaaaacc cgttttgctt tgcgtaatgg tgtcaatctt cagccttttg
     2161 ccgcttttaa tgttttgcac aggtcaaaat ctttcggcgt ggaaatggac ggcgaaaaac
     2221 agacgctggc aggcaggacg gcgctcgaag ggcggttcgg cattgaagcc ggttggaaag
     2281 gccatatgtc cgcacgcatc ggatacggca aaaggacgga cggcgacaaa gaagccgcat
     2341 tgtcgctcaa atggctgttt tgatgcgtcg ggaaatgttt tgacgcacag gcggcacacc
     2401 ggcacggccc cgcgcgccgc cccgcaaacc aatccgaacc ctgccgcccc gaagggcggg
     2461 gcatcatgat gaaaccggcg gaaaaccgcc ggttttttgc cgccgtttga aacccgattc
     2521 tggcttcaga cggcattgtc gcggcatcgg gcggcagggt ttggaacagc ggcataaaaa
     2581 actgatacaa tccgccgatt gataatggtt attttttatt tttgtgggaa gacatttatg
     2641 cctgcacgaa acagatggat actgctgcct ttattggcaa gcgcggcata tgccgaagaa
     2701 acaccgcgcg aaccggattt gagaagccgt cccgagttca ggcttcatga agcggaggtc
     2761 aaaccaatcg acagggagaa ggtaccgggg caggtgcggg aaaaaggaaa agttttgcag
     2821 attgacggag aaaccctgct gaaaaatccc gaattgttgt cccgcgcgat gtattccgca
     2881 gtggtctcaa acaatattgc cggtatccgc gttattttgc cgatttacct acaacaggcg
     2941 cagcaggata agatgttggc actttatgca caagggattt tggcgcaggc agacggtagg
     3001 gtgaaggagg cgatttccca ttaccgggaa ttgattgccg cccaacccga cgcgcccgcc
     3061 gtccgtatgc gtttggcggc agcattgttt gaagacaggc agaacgaggc ggcggcagac
     3121 cagttcgacc gcctgaaggc ggaaaacctg ccgccgcagc tgatggagca ggtcgagctg
     3181 taccgcaagg cattgcgcga acgcgatgcg tggaaggtaa acggcggctt cagcgttacc
     3241 cgcgaacaca atatcaacca agccccgaaa cggcagcagt acggcaaatg gactttcccg
     3301 aaacaggtgg acggcacggc ggtcaattac cggctcggcg cggagaaaaa atggtcgctg
     3361 aaaaacggct ggtacacgac ggcgggcggc gacgtgtccg gcagggttta tccggggaat
     3421 aagaaattca acgatatgac ggcaggcgtt tccggcggca tcggttttgc cgatcggcgc
     3481 aaagatgccg ggctggcagt gttccacgaa cgccgcacct acggcaacga cgcttattct
     3541 tacaccaacg gcgcacgcct ttatttcaac cgttggcaaa ccccgaaatg gcaaacgttg
     3601 tcttcggcgg agtgggggcg tttgaagaat acgcgccggg cgcgttccga caatacccat
     3661 ttgcaaattt ccaattcgct ggtgttttac cggaatgcgc gccaatattg gatgggcggt
     3721 ttggattttt accgcgagcg caaccccgcc gaccgaggcg acaattttaa tcgttacggc
     3781 ctgcgctttg cctgggggca ggaatggggc ggcagcggcc tgtcttcgct gttgcgcctc
     3841 ggcgcggcga aacggcatta tgaaaaaccc ggctttttca gcggttttaa aggggaaagg
     3901 cgcagggata aagaattgaa cacatccttg agcctttggc accgggcatt gcatttcaaa
     3961 ggcatcacgc cgcgcctgac gctgtcgcac cgcgaaacgt ggagtaacga tgtgttcaac
     4021 gaatatgaga aaaatcgggc gtttgtcgag tttaataaaa cgttctgatt gctgttcctt
     4081 ttcggaggaa accctgccgg cggcggtatc acggcgggca tcggcggctt tcgggcggtg
     4141 ctttgcgtgc cgccgcgtgt gcggaaacgc attccggttt ttccggcata acggcgatgc
     4201 gaggtaaaat gccgtctgaa acccgattcg ggcttcagac ggcattgtcg cggttgcggc
     4261 aggcgggttc accagattcc gtcaaaggtt ttcgcgccgc gccaaaattt ccacctgtcg
     4321 gcgggtttga aggtcagcgt accgccgtgt tgtccgtccg tggtgatgtc cagccgtttg
     4381 attttgccgg tacggacggc ttcgtagatc ggtgcgaacc agcgttcttc ccactgctgc
     4441 aatattgccg cataccgctc cctgtcccct gtcagggcgg tcaggcgcaa atcgtccata
     4501 aacaggatat ggtgcgtgtc gggtaggtat gccgccgttt cttcataggc gcggaagttg
     4561 tcgggcaatg cgcggcggtc ggggtggaaa cggctccaaa ccgtatcggc gaaaagcgtg
     4621 ccgccttgac tgcttccgtg cagactgtcc caaagccaca gcccgttcaa ctcgggcagc
     4681 ccgcgtttct tgcggttatg gttgacgggg tgcgccgcca gccacatttg gatttcggtt
     4741 tggacgcgca gccattccaa cgcgtcttcc ccgtccggct gatcgtccgc gcccaacaat
     4801 ccgcccaagt ccaaaacggg cttcgcgccc cagcggtacg cgcaaggaag ggaaaccagc
     4861 cataattcgg gcaggacggg aacgaaacgc catggaatgt cgccgtaaaa cgccgacagg
     4921 tcgcggcaga gccgttccgc ttcatccgta ccgacgttca gatattccgc cgttagcaca
     4981 tttgcctgat gcatccccat cttttgccag acgggcgtgg cgagcgcgac ggcttcagac
     5041 ggcatattca ggctttgtgc cgcgcgttcc gccagtctgc cgcaccacaa ataacgcgcg
     5101 taaaatgccg aagccgtgca gctttggcgg tgcagcgagc cgtattgcag gattttgttg
     5161 aaggcgggca ggcagagagg tattcgggtt tcgtcttcat ccaaattgag cgagggaatg
     5221 gcgagggtga gtttcatcgt ttgacgtttc agaaatgcag gtcaggcgca acattataga
     5281 ggattcggcg caaacgccgt caaaaaggaa caatatggct gtcttcccac tttcggcaaa
     5341 acatcggaaa tacgcgctgc gcgcgcttgc cgtttcgatt attttggtgt cggcggcata
     5401 cattgcttcg acagagagga cggagcgcgt cagaccgcag cgcgtggaac aaaaactgcc
     5461 gccgctgtct tggggcggca gcggtgttca gacggcatat tgggtgcagg aggcggtgca
     5521 gccaggcgac tcgctggcgg acgtgctggc gcgttcgggt atggcgcggg acgaaattgc
     5581 ccgaataacg gaaaaatatg gcggcgaagc cgatttgcgg catttgcgtg ccgaccagtc
     5641 ggttcatgtt ttggtcggcg gcgacggcgg cgcgcgcgaa gtgcagtttt ttaccgacga
     5701 agacggcgag cgcaatctgg tcgctttgga aaaaaaaggc ggcatatggc ggcggtcggc
     5761 ttctgaggcg gatatgaagg ttttgccgac gctgcgttcg gtcgtggtca aaacgtcggc
     5821 gcgcggttcg ctggcgcggg cggaagtgcc cgtcgaaatc cgcgaatcct taagcgggat
     5881 tttcgccggc cgcttcagcc ttgacggttt gaaggaaggc gatgccgtgc gcctgattta
     5941 cgacagcctg tatttccacg ggcagcaggt ggcggcgggc gatattttgg cggctgaagt
     6001 cgttaagggc ggcacaaggc atcaggcgtt ctattaccgt tcggacaagg aaggcggagg
     6061 gggcggcaat tattacgatg aagacggcag ggtgttgcag gaaaaaggcg gcttcaacat
     6121 cgagccgctg gtctatacgc gcatttcttc gccgttcggc taccgcatgc accccatcct
     6181 gcacacttgg cggctgcaca cgggcatcga ttatgccgca ccgcagggaa cgccggtcag
     6241 ggcttccgcc gacggcgtga ttacctttaa aggccggaag ggcggatacg gcaacgcggt
     6301 gatgatacgc cacgccaacg gtgtggaaac gctgtacgcg cacttgagcg cgttttcgca
     6361 ggcacaaggc aatgtgcgcg gcggcgaggt catcggtttt gtcggttcga cagggcgttc
     6421 gaccgggccg cacctgcatt acgaggcacg catcaacggg cagcccgtca atcctgtttc
     6481 agtcgcattg ccgacacccg aattgacgca ggcggacaag gcggcgtttg ccgcgcagaa
     6541 acagaaggcg gacgcgctgc ttgcgcgctt gcgcggcata ccggttaccg tgtcgcaatc
     6601 ggattgaagt ttgaaccggc gacgaaaaca atgccgtctg aaaatctgca aacaggtttt
     6661 cagacggcat ttatagtgga ttaacaaaaa ccagtacgtc gttgcctcgc cttagctcaa
     6721 agagaacgat tctctaagat gctgaagcac caagtgaatc ggttccgtac tatttgtact
     6781 gtctgcggct tcgttgcctt gtcctgattt ttgttaatcc accatacagt cgattaaaat
     6841 aaaactaagc caaggaagca ctgccgtcat tcccgtacgg gcgggaatcc ggacaccacg
     6901 gtacggaaac ccatccgccg tcattcccgc gaaagcggga atctagaaat gcaacgcggc
     6961 aggagtttat cggaaatgat ggaaacccaa cgcaccggat tcccactttc gcgggaatga
     7021 cgaagtaggt aggaatccgg atttatccgt tccgacagtg tttgcaaata aaagaaaacc
     7081 caaccgtccc gattcccggc agggctgttt tacggatttt gcagcgaggg cgcggggcgg
     7141 tcttgcgcct gtttggtttg cagggtcgtc agttttttcg tcagcagatt cagaatcacg
     7201 ccgtaggcgg gcaggaaaaa cagaccgcag acggtgagtt tgaacaggta atcgacaaaa
     7261 gcgatgccct gccagtttgc cgccataaat tcatcgctgc ttgcatagaa ggcaacggcg
     7321 aaaaatacca acgtatccaa ggcgttgccg atgacggttg atgcggtcgg tgcaatccac
     7381 cacgctttca gacgacgtaa tttgttgaac acaaaaatat caaggatttg tccgagcgcg
     7441 taggcggcaa agctggccaa ggcgatgcgt ccgacaaagg tgttgaattc ggatagtgcg
     7501 cccaagcctg tccaactgcc gttgtggaac aaaacggaaa agacgtagga aagcaaaagg
     7561 gcggggaaca tcacccaaaa gataatccgc cgtgccaagt gcgaaccgaa aatgcggacg
     7621 gtcaggtcgg tggcgaggaa gatgaagggg aaggaaaacg caccccaagt ggtgtggatg
     7681 ccgaaaattt ggaaggggaa ctgcaccaga tagttgctgg cggcgatgat gaggatatgg
     7741 aaaagcacca gccagaagag tgccttctgt tgctgtgcgg cagtaaatgc gtacataaaa
     7801 atctttcgga aaggcgttca gacggcatac cgtatcgaag gaatgccgtc tgaaatatgg
     7861 gaaggatggt ttattgtgcg tcgtgctcaa acaggcgttt acgcgccaat gcttcaaact
     7921 cggtgcctgc tttgccgtaa ttggcgaaag gatgaatggc gatgccgccg cgcggtgtga
     7981 actcgccgaa tacttcgatg tatttcggat ccatcagggc aatgaggtct ttcatgatga
     8041 tgttgacgca gtcttcatga aaatcgccgt ggttgcggaa gctgaagagg tagagtttca
     8101 gggatttgct ttccaccatt ttgatgtgcg gaatgtagcg gatgtagatg gtggcgaagt
     8161 cgggctgccc ggtcatgggg cagaggctgg tgaactctgg gcagacgaat ttgacgaaat
     8221 agtcgttgtc gggatgtttg ttgtcgaatg cttcgagaat ttcaggcgcg tagccggtcg
     8281 gatatcgggt tttctgatta cccaaaagcg agatgccttg cagctcttcg gtattgcggg
     8341 acatagggtt tccttagttt tttagtgtgg gaggttttcg aaccacgggg cggcgattgt
     8401 aatataagcg gcggtatctg tgtagttttc ttcagacggc atggtttgga cggcggcgtt
     8461 ttccgt
//
LOCUS       DAWKOG010000082         8408 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.082, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000082 DAWKOG010000000
VERSION     DAWKOG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8408
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.082"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(308..1240)
                     /gene="cysK"
                     /locus_tag="WJK66_001341"
     CDS             complement(308..1240)
                     /gene="cysK"
                     /locus_tag="WJK66_001341"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEZ7579049.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVSNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(1434..2039)
                     /locus_tag="WJK66_001342"
     CDS             complement(1434..2039)
                     /locus_tag="WJK66_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579050.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(2036..2887)
                     /gene="dapF"
                     /locus_tag="WJK66_001343"
     CDS             complement(2036..2887)
                     /gene="dapF"
                     /locus_tag="WJK66_001343"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEZ7579051.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAAWADRFRGVG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     ANGVIFPKLSDNGMVTVNMGKPRFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAQVRETGSLIEPHRQFPERVNVGFMQIVSRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVILPGGTLYIEWACGGDVMMTGPAETVF
                     EGELAYS"
     gene            3024..3320
                     /locus_tag="WJK66_001344"
     CDS             3024..3320
                     /locus_tag="WJK66_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579052.1"
                     /translation="MFRILLLLFPCAILAACTRNIPANPAVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANRILIQDFNSTGNALGGFYVPDCRKSSLSPKNI"
     gene            3334..4002
                     /locus_tag="WJK66_001345"
     CDS             3334..4002
                     /locus_tag="WJK66_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ7579053.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(4075..6195)
                     /gene="pnp"
                     /locus_tag="WJK66_001346"
     CDS             complement(4075..6195)
                     /gene="pnp"
                     /locus_tag="WJK66_001346"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEZ7579054.1"
                     /translation="MFDKHVKTFQYGNHTVTLETGEIARQAAAAVKVSMGDTVVLVAV
                     TTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRPL
                     FPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYVNGV
                     YVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAINA
                     INEFADEVNPEVWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWSA
                     VKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTGV
                     LPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEVG
                     RMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCLS
                     LLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQM
                     DIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKIR
                     EVIGKGGETIRAITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGKV
                     YEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEVD
                     DRGRVRLSIKALLDAPAREENAAE"
     gene            complement(6432..7295)
                     /locus_tag="WJK66_001347"
     CDS             complement(6432..7295)
                     /locus_tag="WJK66_001347"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEZ7579055.1"
                     /translation="MSEIGLVKIYFGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLVHIMPNHFTGQTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIQLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECERIIGKELAAEVRAKAIQLYTEAAEYAKSRGIIICDTKFEFGLDTNGTLTLMDE
                     VLTPDSSRFWPADQYKVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            7495..8358
                     /gene="rfbD"
                     /locus_tag="WJK66_001348"
     CDS             7495..8358
                     /gene="rfbD"
                     /locus_tag="WJK66_001348"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEZ7579056.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGNAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     IFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAAHNQIGCPTYAGDLAAAIIRLLQQTSPVRGIYHYAGGKSVSWYE
                     FAQHIFQAASQQQTSFPVPELTAVSDKEYPTAAPRPAYSILDCRKIENDFSIKPSDWQ
                     KALAQVVSKLL"
BASE COUNT         2008 a         2426 c         1944 g         2030 t
ORIGIN      
        1 gtggattaac aaaaaccagt acagcgttgc ctcgccttgc cgtactatct gtactgtctg
       61 cggcttcatt gccttgtcct gatttttgtt aatccactat atctgtactg tctgcggctt
      121 cgtcgccttg tcctgatttt tgttaatcca ctataaaaaa tatttccctc aagcgatgtg
      181 gcggcaccaa cacctgatta aaacacaacc ggcgttttcg gcatatacgc aaaaacgcat
      241 tttccggcac ggcgttacac cgataccgaa aaatgcgtct gaatgtttcg acaatccgat
      301 taaagcatta tgccaaatct gcaaaaagtg gcgtagagag atagcgttcg ccataagaag
      361 gcagcagcac gactatcagc ttgccttcgt tttcaggctg tttggcaagc tgcaacgcac
      421 tccaaaccgc cgcaccggaa gaaataccca ccaaaatgcc ttctttttcc gctattgcgc
      481 gggcggtttc aaaagccgct tcgttcgaca ctttggtaat gctgtcgtaa attttggtat
      541 tcaaaacggt cggaataaag cctgcgccga tgccttgaat cgggtgcggg cctttttcgc
      601 cgccgctcaa tacgggtgaa gcttcaggct cgacggcaac cactttaact tcgggtttgt
      661 attttttcaa cacttcgccc acgccggtaa tcgtaccgcc cgtgccgacg ccggcaacga
      721 agacatcgac tttgccgtcc gtatcccgcc aaatttcctc ggcggttgtt ttgcggtgga
      781 cttcggggtt tgcctcattg tcgaactggc gcggcataaa ataagtgtcg ggatgcgcgt
      841 ccaccaagga tttcgctttg gcaatcgcgc ccgccatacc ttcggcggca ggggtcagaa
      901 tcagctccgc accaaacgcg cgcaacagca ttttgcgctc cttactcatg ctttccggca
      961 tggtaatcgc cagcttgtag ccgcgtgcgg cacataccat tgccaaaccg acacccgtat
     1021 tgccgctggt tgcttcgaca atgacggtgt ttttgttgat tttgcccgct ttttcggcac
     1081 cctcgatcat tgcttcggca atgcggtctt tgacgctgct gcctggattg aaaaattcca
     1141 gtttcacggc aacctccgcc ttcaaacctt cggtcagacg gttcagtttg accaaaggcg
     1201 tgttgccgat tagttcggtg atgctgtttg caattttcat cttctgctcc tgaataataa
     1261 ggtttcgaca gtaaggcgca atccccatag gcatcctgtc cgattgcagg ggatttgcgc
     1321 cgaaacataa acaaatttcg gatatggctt tatcctaaac ctatgccgcc taaaaaacca
     1381 atatccaaaa tttctttctt tataactttt tgttccgata cggccgcacc gtattatctt
     1441 ccaatttctg aaagcagcag caccagtacc gagacctgca tcaacgctgc cgtcaacccg
     1501 ccgagtcgca gctgcgagcc gccgaacgtt gacggcggct gccggtctat accgcagcgg
     1561 tggacatagg caaccgccat cagcacaaac tgcaacacga accaataaac cggcttcagc
     1621 gcataaagtt tgagcagtgc cgcaaacaca aacacttgaa ccgttcccga aaagcaataa
     1681 tgcaccaacg cacatattcc gacaaaagca agcgtcatcg atacattact gacggcaaac
     1741 agcccgagca gaggatgttc ggggtctgcc tgccatccgt ttccatccgt tttccggttc
     1801 caatgcccga tgaaaaaaaa tatgctgccc aaagtcaggt aaaaatgggg gatgaacaag
     1861 ggcgcggcgc gggtcattcc ccatatgccg ggcatcagct ttgcccccaa aaccgatatg
     1921 cccagccaca gcataatact cgcccaaaac cattgcaaca ttcctgcgcg gaatatcaga
     1981 aacagaaaaa ccgccgcata cagtaaggca gacaaaaaat ccaaatgcag caaaatcatg
     2041 aatacgccaa ctcgccttca aacaccgttt ccgcagggcc ggtcatcatc acatcgccgc
     2101 cgcaggccca ttcgatatat aaagttccgc ccggcaaaat cacctctacc gttttccctt
     2161 catccaacaa ccccagacgg atacccgcca ccacagccgc acacgcgccc gtaccgcacg
     2221 cttgggtttc gcccacgccg cgctcgaaca cgcgcaaacg aatcgcggtt cggctgacaa
     2281 tctgcataaa gccgacgttg acgcgttcgg gaaactgcct gtgcggttcg ataagcgaac
     2341 cggtttcgcg cacctgcgcg cattctacgt catcgaccac aatcaccgca tgggggttgc
     2401 ccatattgac gcagctgaca ggctgaatgc cggattcgag atgcaccccg taaatacagg
     2461 catcatcccc ctcgcccgat tcggggacaa acggtatttc agacggcata aacctcggtt
     2521 tgcccatatt gaccgtaacc atgccgttat cggacaattt cggaaaaata acgccatttg
     2581 ccgtttcaac acaaatttct ttcttatcgg tcaaaccctt gtctgcaaca aaacgggcaa
     2641 aacaacgcgc cccgttgccg cactgcccga cctcgctgcc gtcggcattg aaaatacggt
     2701 aacggaaatc cacgccttcg gtttccgaac gcccgaccac caaaagctgg tcgaagccca
     2761 cgccccggaa acggtccgcc catgccgcaa tcggcgcgtc ctcgggggta aaatcctgac
     2821 tgaccgcgtc aatcaccata aaatcgttgc ccaaaccgtg cattttggta aatttcaaag
     2881 tgttcatatt gttccgaaat cccgtatcgt ctgttgcttc cgaatccgaa atcccccgca
     2941 ccgaagggaa atgcttccat ctcccgccgc ctgaggatta aaatcagcca ttatactgtt
     3001 tgttacccct tacaagaaac accatgttcc gcatattact gttgcttttc ccttgtgcaa
     3061 tccttgccgc ctgcacccga aacataccgg caaacccggc cgtttcaaat tgcgcgcatc
     3121 ttcaaaaccg caccatcctg tattttgatg cacacggcaa acccgccgac ggcgcagtaa
     3181 agggcggatt ttacaaaatt atcgaaatac agggtgcaaa ccgcatcctt attcaagact
     3241 ttaacagcac gggcaatgcc ctcggcggat tctatgtgcc ggactgccgg aaatcatccc
     3301 tttcaccgaa aaatatctga cttcaaactg cctatgaacc acgacatcac tttcctcacc
     3361 ctgttcctac tcggtttctt cggcggaacg cactgcatcg gtatgtgcgg cggattaagc
     3421 agcgcgtttg cgctccaact ccccccgcat atcaaccgct tttggctgat cctgctgctt
     3481 aacacaggac gggtaagcag ctatacggca atcggcctga tactcggatt aatcggacag
     3541 gtcggcgttt cactcgacca aacccgcgtc ctgcagaata ttttatacac ggccgccaac
     3601 ctcctgctgc tctttttagg cttatacttg agcggtattt cttccttggc ggcaaaaatc
     3661 gagaaaatcg gcaaaccgat atggcggaac ctgaacccga tactcaaccg gctgttaccc
     3721 ataaaatcca tacccgcctg ccttgcggtc ggaatattat ggggctggct gccgtgcgga
     3781 ctggtttaca gcgcgtcgct ttacgcgctg ggaagcggta gtgcggcaac gggcgggtta
     3841 tatatgcttg cctttgcact gggtacgctg cccaatcttt tggcaatcgg cattttttcc
     3901 ctgcaactga aaaaaatcat gcaaaaccga tatatccgcc tgtgtacggg attatccgta
     3961 tcattatggg cattatggaa acttgccgtc ctgtggctgt aagccccatt cattcccgat
     4021 acccgtaaaa aataccgtct gaaacctgtt cagacggcac tttcacccta agcgttactc
     4081 ggcggcattt tcctcacggg caggcgcgtc cagcagggct ttgatggaca gacggacacg
     4141 gcctctgtcg tccacttcca atgctttcac gttcaccacc tgaccgactt gcaggtagtc
     4201 gccgacattg cgtacgcgct cgtgggcgat ttggctgatg tgtaccaaac cgtctttgcc
     4261 cggcatcaca ctgacaatcg cgccgacatt gttgtcgagg attttcacca cagtgccttc
     4321 gtacactttg cccacttcca cttcggcagt aatctgctcg atgcgttttt tcgccgcatc
     4381 gccggcttct tgagtggttg cggcaatggt aatcgtaccg tcttcggcaa tattgatttc
     4441 cgtaccggtt tcagcggtaa tcgcacggat ggtttcaccg cccttaccga taacttcgcg
     4501 gattttgtct tggttgattt tcatcgtgaa caagcgtggc gcgtgtgcgg acagctcttg
     4561 cgggcccgca acggcggctt tcatctgatc caagatgtgc agacgcgctt ctttggcctg
     4621 cgccaaagcg atttgcataa tctctttggt aatgccttgg attttgatgt ccatttgcag
     4681 cgcggtaacg ccttcggtcg taccagccac tttaaagtcc atatcgccca agtggtcttc
     4741 gtcgcccaaa atgtcggtca ggacggcaaa cttattgcct tccagaatca gacccatcgc
     4801 gataccggca acgtgtgctt tcaaaggcac gccggcggac agcaggctca agcagccgcc
     4861 gcagacggaa gccatagagg aagagccgtt ggattcggta atttcggaga ccacgcgcat
     4921 ggtgtagctg aaatcttcag gtttcggcaa tacggccaac aatgcacgtt tagccaaacg
     4981 accgtgaccg atttcacggc gtttcggcgc gcccatgcgg cccacttcgc cggtagagta
     5041 cggcggaaag ttgtagtgca gcataaagcg gtcggtgtat tcgccggaca gcgcgtcgat
     5101 gatttgctcg tcgcgcgaag tacccaaagt cgctacggca agggcttggg tttcgccacg
     5161 ggtaaacaat gcggaaccgt gcgtgcgcgg caatacgccg gtttggatgt tcagcggacg
     5221 gacggtgcgg gtgtcgcggc cgtcgatgcg cggttggccg tccaaaattt ggctgcggac
     5281 gacatcggct tccaagtgtt tgaaaatgcc tttgatttcg ttggctgcca gcgtgtcggt
     5341 ttcttcggta atcaaggctt cttttaccgc actccaagct tcgtccaatt tggcagaacg
     5401 cgcttgtttt tgacggattt tgaacgcttc tttaatggtt tcgccggcaa tcccgcggac
     5461 tttggcaacc agttcctcat tggtttcagg tgctttccaa tcccaaactt ccggattgac
     5521 ttcgtcggca aattcattga ttgcattgat ggcaacctgc atttgatcgt ggccgtaaac
     5581 caccgcgccc agcatcacgt cttcgggcag gattttggct tcggattcca ccatcaacac
     5641 ggctttggaa gtaccggcga ccaccaagtc caattgcgat ttcgccaatt cggctttagt
     5701 cgggttcaaa acgtacacgc cgtttacata accgacgcgt gccgcgccga tcgggccggc
     5761 aaacggtacg ccgctcaaca ccagcgcggc agacgcgccc aacattgcag gaatatcaga
     5821 atcgatttca ggatcgacgg acacgaccat cgctacgatt tggatgtcgt ggtagaaacc
     5881 ttcagggaac agcggacgaa tcggacggtc gatcagacgg ctggtcagga tttctttttc
     5941 gctttgtttg ccttcgcgtt tgaagaaacc gccgggaatt ttgcctgcgg cgtaagtgcg
     6001 ctcgaggtaa tcgacagtca gagggaagaa gtcttggcct tctttcactt ctttgttggt
     6061 ggtaacggca accaaaacca cggtgtcgcc catagagact ttaacggcag cggcggcttg
     6121 gcgggcaatt tcgccggttt ccaaagtaac ggtgtggttg ccgtattgga aagttttaac
     6181 gtgtttgtcg aacattattg ttcctttcaa aataccgcac tgctaaaaca ctaataatgc
     6241 acactaaaat ccgaatgtgc atagttaggg tttcagaccg tgcggcaggt tgtaaacaag
     6301 ctttcagacg acatttcgga cgctgaaagc aattccaaat tataaaggca accatcatta
     6361 aaatccagta ttgatacaat aaaaaggccg tctgaaccat atttctttca gacggccttc
     6421 aaacttaaaa atcaatcctg agtcagcaaa gtcaatgctt cccgatactt ctcgacagtt
     6481 ttctgaatca catcggcagg cactttcggc gcaggggctt ttttgttcca accgctttgt
     6541 tccagccagt cgcggacgaa ttgtttgtca aaagacggcg gattggtgcc gactttgtat
     6601 tggtcggcag gccaaaaacg gctcgaatcg ggagtcaata cctcatccat cagcgtcagc
     6661 gtaccgtttg tatccaaacc gaattcaaat ttggtatcgc aaatgatgat gccgcgcgat
     6721 ttggcatatt ccgccgcttc tgtataaagc tgaatcgctt tggcgcgcac ttccgccgcc
     6781 aattcttttc cgataatgcg ttcgcattct tcaaagctga tgttttcatc gtgatcgccg
     6841 actgcggctt tggttgaggg cgtaaaaatc acttcaggca gttgttgcgc ttcctgcata
     6901 ccttcaggca gttgaatacc gcaaaccgag ccggtttttt gataatcttt ccaaccgctg
     6961 cctgccagat aaccacgcac aatcgcctct actttcaccg gagtgagctt tttagccacg
     7021 acggcgcgtt tctctaaagc tttggcttcg ttttcaggca aaacatcgta aaccgtttga
     7081 ccggtaaagt ggttgggcat aatatgcacc agttttttaa accaaaaatt ggaaatctgc
     7141 gtcagaatct cccctttgct cggaatcggg tcgtccaaaa tcacatcgaa tgcagacagg
     7201 cggtcggaag cgaccatcag catacgttta tcgtcgattt catataaatc gcgcactttt
     7261 ccaaaataga tctttaccaa accaatctca ctcatttcgc ccccccctga aaatatcttg
     7321 aaaataccga cccgacaccc gacaggtttg aatcacaaac caatattcta gccgaagtcg
     7381 gcgcaaaaca atacccatgg cacaaaaaac caacccgtca accgtcggca aagttttggc
     7441 actataatac cgacagcaag tcctacaata cacttttacc aaaggaaata cctcatgaga
     7501 atcctattga caggctcaaa aagccaactg gcacgctgcc tgcgcgaccg tcttccggaa
     7561 gactgggaaa ccattgcgac ggattccgca tccctagaca ttaccgatgc cgatgccgtc
     7621 tgcaacatgg tcaaaagttt ccaacccgac gctattgtca acacggctgc ctatactgcc
     7681 gtcgacaaag cggaaggcaa tgcggcagcg gcatttgccg tcaatgcttc cgccgtttac
     7741 aaccttgcct tggcagcaca tcgcgcccat gcccgattca tccacatctc aaccgactat
     7801 atctttgacg gtaaagggaa aaggccctat caggaaagcg actttaccaa tccttccaat
     7861 gtatacggac aatccaaaac cgcaggcgag ctgctcgcac tgtctgccaa tcccgacagc
     7921 cttatcctgc ggacttcttg gctgtttagc gaatacgggg acaactttgt ccgcaccatg
     7981 ctgaaccttg cgcgggaacg ttccccgctg tccgccgccc acaaccaaat cggctgcccg
     8041 acctatgccg gcgatttagc tgccgccatc atccgccttt tgcagcaaac gtcgcccgtg
     8101 cgcggcattt accactacgc cggcggcaaa tccgtatcct ggtacgaatt tgcccaacat
     8161 attttccaag cggcatcgca acagcagaca tccttccccg ttcccgaatt gactgccgtt
     8221 tcagacaagg aatatccgac cgccgccccc aggcccgcat acagcatttt ggactgccgc
     8281 aaaatcgaaa acgacttcag catcaaaccg tcagactggc aaaaagccct tgcacaggtc
     8341 gtttccaagc tgctctgatg ccgcccgcct ctccgtttcc gccgtcaagc accgccttgg
     8401 cggttttc
//
LOCUS       DAWKOG010000083         8389 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.083, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000083 DAWKOG010000000
VERSION     DAWKOG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8389
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.083"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(354..629)
                     /locus_tag="WJK66_001349"
     CDS             complement(354..629)
                     /locus_tag="WJK66_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ7579057.1"
                     /translation="MNASNWSVYLILCENSAFYCGISTNPQQRLDAHTAGKGAKYTRV
                     FKPVEMRIVAGGMDKGTALRQEIAVKKLTAAQKRKLWEQAEKMPSET"
     gene            complement(626..1909)
                     /locus_tag="WJK66_001350"
     CDS             complement(626..1909)
                     /locus_tag="WJK66_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ7579058.1"
                     /translation="MTVSKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRVAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLAREPVLPPAVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAVTVLGFVWLAGFGHFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFVLALPGMLLLLKVAPWNGGKTQDTGR"
     gene            complement(2063..3481)
                     /gene="glnA"
                     /locus_tag="WJK66_001351"
     CDS             complement(2063..3481)
                     /gene="glnA"
                     /locus_tag="WJK66_001351"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEZ7579059.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDVTVVITCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDCGQDLRSAMVNILEGLGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            3565..3714
                     /locus_tag="WJK66_001352"
     CDS             3565..3714
                     /locus_tag="WJK66_001352"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579060.1"
                     /translation="MPSETRPASNFKLENIVSDGKIVNNMLRFVCSKAEYPPFCPNAD
                     KTDAA"
     gene            3792..4601
                     /gene="aroE"
                     /locus_tag="WJK66_001353"
     CDS             3792..4601
                     /gene="aroE"
                     /locus_tag="WJK66_001353"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEZ7579061.1"
                     /translation="MHALPRYAVFGNPVAHSKSPQIHQQFALQEGVDIEYERICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSERALAAGAVNTLIFLKNGKLRGD
                     NTDGIGLANDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTHAKA
                     EELARLFGIEAVPMADVNGGFDIIINGTSGGLSGQLPAVSPEIFLGCRLAYDMVYGDA
                     AQEFLNFAQSNGAAEVSDGLGMLVGQAAASYALWRGFTPDIRPVIEYMKAM"
     gene            4603..5304
                     /gene="mtgA"
                     /locus_tag="WJK66_001354"
     CDS             4603..5304
                     /gene="mtgA"
                     /locus_tag="WJK66_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEZ7579062.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHQTAFMSMR
                     MKQFEQEGRDVALDYRWMPYNRISVNLKKALIASEDARFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYQIPAAKLTKQQAAKLTARVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(5628..5703)
                     /locus_tag="WJK66_001355"
     tRNA            complement(5628..5703)
                     /locus_tag="WJK66_001355"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5668..5670),aa:Phe,seq:gaa)
     gene            complement(5894..6628)
                     /gene="lptB"
                     /locus_tag="WJK66_001356"
     CDS             complement(5894..6628)
                     /gene="lptB"
                     /locus_tag="WJK66_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEZ7579063.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVVGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVMLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEISTKDKSRIDGEVEKLLADLNIERLRNNPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGTV
                     LASGKPDDLVGNEQVRSVYLGKNFKY"
     gene            complement(6702..7232)
                     /gene="lptA"
                     /locus_tag="WJK66_001357"
     CDS             complement(6702..7232)
                     /gene="lptA"
                     /locus_tag="WJK66_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEZ7579064.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLDQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVVIQPSSTQKSE"
     gene            complement(7213..7794)
                     /gene="lptC"
                     /locus_tag="WJK66_001358"
     CDS             complement(7213..7794)
                     /gene="lptC"
                     /locus_tag="WJK66_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEZ7579065.1"
                     /translation="MKVRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSSKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGMLNFSSKVKATIYDTKDM"
     gene            complement(7791..8327)
                     /locus_tag="WJK66_001359"
     CDS             complement(7791..8327)
                     /locus_tag="WJK66_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ7579066.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
BASE COUNT         1972 a         2265 c         2159 g         1993 t
ORIGIN      
        1 attatggtag atactaactc tttaagttga catattgtgt gatatcgagg gctgtaccgt
       61 attttcccat cgtattttcc cgttctttat atgagcggca ggcatcctca taaaagaagg
      121 gataccgata aggaaaaata ccccggcata aaaaatatac tgattaatca atacaaatcc
      181 aacaaacagt tcatcgtatt cttttctgtt ggcgtataaa aaatactgat atatgccttc
      241 actttcatag ggcgacgctt tatgtaccgt cctgtgtgtt gaaacgtcgg ttttccttac
      301 cgcctgcccc gcttttccct gattgctgcc aactgccgtc cgaacctgaa ccgtcaggtt
      361 tcagacggca ttttttctgc ctgttcccac aatttccgtt tttgtgcggc ggtcagtttt
      421 ttgacggcga tttcctgcct gagtgccgtg cctttatcca tcccgcctgc aacgatacgc
      481 atctccaccg gtttgaatac gcgggtatat ttcgcgcctt ttccggccgt gtgggcgtca
      541 agccgctgtt gcggattcgt gctgatgccg caatagaacg cgctgttttc acacaatatc
      601 aggtaaacgc tccagtttga cgcgttcatc tgcctgtatc ctgagttttt cccccgttcc
      661 aaggcgcaac tttcagcaac aacagcatac ccggcagggc aagtacgaaa cacaactgga
      721 aaaacggtac ataaccgagc cattcgatca gataaccggc aaaggaattg atgaccgtac
      781 ggggaacagc ggacaggctg gtaaacagtg cgagctgcgt tgcggtaaat gcgggattgg
      841 tctcgcgcgc catatacgat acgaacgccg ccgtccccaa acccacgccg actgcctctg
      901 cgccgataac tgccgccagc atcagcctct cgcctgtgcc gaccgtgtcg aaatgtccga
      961 accctgccag ccatacaaac cccaaaacgg ttacagcctg caccgcgcca aatagccaca
     1021 aggctttgtt tacgccgatt ttcagcatcc acacgccgcc caagatacct gccgccactg
     1081 ccggccacag tcctgcattt ttcgcaatca aaccgatgtc tgtcttgctg aaacccatat
     1141 ccagataaaa cggcgttgcc aacgcggttg ccatactgtc gccgagtttg taaaggaaga
     1201 taaacagcag cacgcacacc gccgaagcga tgcccttgcg cgtaaaaaat tctttaaacg
     1261 gctctaccac ggtctgcttc aacgttttag gaacggcagg cggcaacacg ggttcgcgcg
     1321 caagaaacag cgtcatcaga aggccgggca gcataaataa tgaagtgata acaaatactt
     1381 ctgaccacgg catcctgtct gccaacacca aactcaatga accgggaatc agggcggcaa
     1441 cccgataggc gttcacatga accgagttgc ccaaacccaa ttcttcgtcc gacaaaatct
     1501 cgcgcctgaa cgcatccaat acaatgtcct gactggcgga aaaaaaagcg acaagcaccg
     1561 acaagccggc aatcagcggc agatgattac gggggtttaa aaaggcatag gccgccaaag
     1621 ccgccagcaa ccctgcctgc gtcagcagca tccacccgcg ccgccgtccc aaaacgggca
     1681 gcctgaccgc gtccatcagc ggcgaccaca aaaatttcca agtaaacggc agaccgatta
     1741 acgccatcag cccgatgctc ttcaaatcca cctgctcgct gcgcaaccac gccggaatca
     1801 ggttaatcag aaagtacagc ggcagccccg aggtaaaccc cgtaaaaata caaacaagca
     1861 tatttcggga aaagatttgc ccgataaaac ctgatttcga tacagtcatg gttcaaacag
     1921 ccaagaaata aaagcctatt gtagcaaata tcgcccaacc gctgaaaaac accgcccctg
     1981 ctccgccgtt ttgccgttca aatgcaaaat gccgtctgaa actgtgttca gacggcattg
     2041 cttttctgaa aacccgacgt gcttacaggc tgtaatacat ttcaaattcc agcggatgcg
     2101 gtgccatgcg gatgcggcgg acatcttcct ctttaaaggc gatatagctg tcgatccagt
     2161 ctttgctgaa cacgccgccg cgcaggagga attcgtggtc ggctttgagg gcggcgaggg
     2221 cttcttccaa agaggcgcaa acggtcggga cgagcgcgtc ttcttccggc ggcaggtcgt
     2281 acaggttttt atcggcagga tcgcccggat ggattttgtt ttgaataccg tccaaacccg
     2341 ccatcaacag ggcggcaaat gccaaatacg ggttggcggt cggatcgggg aaacgcgctt
     2401 cgatgcggcg cgccttgctg ctgttcacgg acggaatgcg gatggaagcg gaacggtttt
     2461 tggcggaata tgccagtttg gtcggtgctt caaagtgcgg cacgaggcgt ttgtaggagt
     2521 tggtggacgg attggtaatt gcgttcaggg ctttggcgtg tttgatgatg ccgccgatgt
     2581 agtagagcgc ggtgtcgctc aagccggcat agccgtcgcc tgcgaacagg ttttgaccgt
     2641 ctttccaaat ggattggtga acgtgcatac cgctgccgtt gtcgcccata atcggtttgg
     2701 gcataaaggt ggctgttttg ccgaagttgt gggcaacgtt ttgaatcaca tatttcatgt
     2761 cttgggtttg gtcggcgcgt ttgaccaagg tggcgaaacg cgtgccgatt tccatttggc
     2821 tgccggtacc gacttcactg tggtggactt cgacttcgat gccgagtcct tccaaaatgt
     2881 ttaccatcgc ggaacgcaaa tcctgaccgc agtcaatcgg cgcgacgggc gcgtagccgc
     2941 ctttgacggc gggacggtgg ccggtgtttt gaccgtccat atgcaggccg cttgcccacg
     3001 cgccgctttc ggacgtgatt tcgtaacggg ttttgtgcat gtcggtttca aattctacgc
     3061 cgtcgaagac gaagaattcg ggttcggggc cgaagtatgc cgtgtcgccg atgccggaag
     3121 atttcaaata ggcttcggcg cggcgggcga tggagcgcgg gtcgcggtcg taaccctgac
     3181 cgtcggcagg gtcgatgacg tcgcaggtaa tgacgacggt aacatcatca taaaaaggat
     3241 cgacgaaggc tgtagacgca tcgggacgca actgcatatc ggaagcctga atgcctttcc
     3301 agccgccgat agacgaaccg tcaaacgcct gaccgttttc gaaccactct tcggggtctt
     3361 ccaacacgat gcgcgcaggc acggtaaagt ggtgctgctt gcctttggta tcggtaaagc
     3421 gcaaatcgac aaagcgggct tcgctttctt caatcaattt tacggcgttt ttaatagaca
     3481 tttttcagct cctggaaaag ggaaatagca aaatatacgg aacgcggcag attagcccaa
     3541 agccagcatg attctaccac gtccatgccg tctgaaactc gccctgcgtc aaatttcaag
     3601 ctggaaaata tagtttcaga cggcaagatt gtcaacaata tgcttcggtt tgtctgttca
     3661 aaagcagaat acccgccctt ttgtccgaat gccgacaaaa cagatgccgc ctgaaaacag
     3721 tttagacggc atcgttccca tcttcgcgcc gccgttataa aatgaccgcc ccgccttgat
     3781 ggaatatgac tatgcacgcc ctcccccgct acgccgtttt tggcaacccc gtcgcccaca
     3841 gcaagtcgcc gcagattcat caacaatttg cccttcagga aggcgttgac attgaatacg
     3901 aacgcatttg cgccgacatc ggcggtttcg cgcaggcggt ttcgacattt tttgaaacag
     3961 gcggttgcgg ggcaaacgtt accgtaccgt tcaagcagga agcgtttcat ctggcggacg
     4021 agcattctga acgcgcattg gctgccggcg cggtcaacac gctgattttt ctgaaaaacg
     4081 gaaaactgcg cggcgacaat accgacggta tcggtttggc caacgacatc acgcaggtca
     4141 aaaacattgc catcgaaggc aaaaccatct tgcttttggg cgcgggcggc gcggtgcgcg
     4201 gcgtgattcc tgttttgaaa gaacaccgtc ctgcccgtat cgtcattgcc aaccgcaccc
     4261 acgccaaagc cgaagaattg gcgcggcttt tcggcattga agccgtcccg atggcggatg
     4321 tgaacggcgg ttttgatatc atcatcaacg gcacgtccgg cggcttgagc ggtcagcttc
     4381 ctgccgtcag tcctgaaatt ttcctcggct gccgccttgc ctacgatatg gtttacggcg
     4441 acgcggcgca ggagtttttg aactttgccc aaagcaacgg tgcggccgaa gtttcagacg
     4501 gactgggtat gctggtcggt caagcggcgg cttcctacgc cctctggcgc ggatttacgc
     4561 ccgatatccg ccctgttatc gaatacatga aagccatgta atatgttccg catcgtcaaa
     4621 tggctgattg ccctgcccgt cggcatcttt atctttttca atgcctatgt gtacggcaac
     4681 atcattacct accgcgccgt cgcgccccat cagactgcct ttatgtcgat gcggatgaag
     4741 cagtttgaac aggaaggtcg cgatgtcgca ctggattacc gctggatgcc ctacaaccgc
     4801 atctcggtca acctgaaaaa agccctgatt gcttccgaag atgcccgttt cgccgggcac
     4861 ggcggcttcg attggggcgg cattcaaaac gccatcaggc gcaaccggaa cagcggcaaa
     4921 gtgaaggcgg gcggctcgac catcagccag cagcttgcca aaaacctctt cctcaacgaa
     4981 agccgcagct atatccgcaa aggcgaagaa gcggcgatta ccgcgatgat ggaagccgtt
     5041 accgacaaag acaggatttt tgaactgtat ttaaactcaa tcgaatggca ctacggcgtt
     5101 ttcggcgcgg aagccgcgtc ccggtatttt tatcaaatac ccgccgccaa gctgaccaaa
     5161 cagcaggcgg caaaactgac ggcgcgcgtc cccgccccgc tctactacgc cgaccatccg
     5221 aaaagcaaac ggctccgcaa caaaaccaat atcgtgctca gacgcatggg ttcggcagag
     5281 ttgcctgaaa gcgacacgga ctgattgttt cagatatgga aatgccgtct gaactgcggt
     5341 tcggacggca tatgttttct ggtttttata gtggattaac aaaaatcagg acaaggcgac
     5401 gaagccgcag acagtacaaa tagcacggaa tcgattcact tggtgcttca gcaccttaga
     5461 gaatcgttct ctttgagcta aggcgagcca acgctgtacc ggtttaaatt taatccacta
     5521 tacctgcccg acagccgcaa agaccgtatc atacatagca ggcattgcgg tgcgggatta
     5581 ctgccgcaaa cggcaggcaa aaaaataacc gcttcaaggc ggttttttgg tggccagagg
     5641 cggaatcgaa ccgccgacac acggattttc aatccgttgc tctaccaact gagctatctg
     5701 gcctttgtct gtctcgttaa gagatgcgta ttaaaccaaa ttacgggacg acatgcaagc
     5761 atatcgggca atattctttg aaaacaggtt taagtcaatg ttttaaattt gaattaattt
     5821 atgtaaaaac aacataaatc cgtatttttg ccgcctgccc gacgatatta ggtcgcccgt
     5881 ctgaaaaata ttttcaatat ttgaagttct tacccagata aacagaacga acctgttcgt
     5941 ttccgaccaa atcatcaggt tttcccgatg ccaacaccgt gccgtctgaa ataatgtagg
     6001 cccgatcgca gatgctgagg gtttcgcgta cgttgtggtc ggtaattaat acgccgatac
     6061 cgcgcgactt gagaaatccg atgatttttt ggatgtcgat gacggcgatg ggatcgacgc
     6121 cggcaaaagg ctcgtccaac aaaataaaat gcggtttcat ggcaagtacg cgggcgattt
     6181 cgacgcgtcg gcgttcgccg cccgacaaag atggggcagg attattgcgc aaacgctcga
     6241 tattcaggtc ggcaagcagt ttttcaactt cgccgtcgat gcggcttttg tctttggtac
     6301 tgatttctaa aatggcgcgg atgttttgtt cgacggtcat tttacggaaa atagaggctt
     6361 cctgcggtag gtagccgaca cccaggcggg cgcgttcgtg tatgggcagg tgacgcaatt
     6421 cctgcccgtc cagcatcacg ctgcccgcat cggcggcaat gagtccaaca atcatgtaga
     6481 agctggtggt tttacccgca ccgttgggcc cgagcaggcc gacgacttcc ccgctttcga
     6541 tttcaaggga gaagctttta acgacttggc gttttttgaa acttttttgc aggttttgaa
     6601 caacaaggcg gctgacgttt gcgctcataa atatctcttc aatatcttcg gtcggcaaat
     6661 gccgtccgaa ccaggtttat gtttcagacg gcattttggg attattcgga tttttgcgta
     6721 ctcgaaggct ggataacgac gctgaccctg ccggatttgg aagcggattt tgcgccggat
     6781 tttgtgctgc cgctgatggt atagacttcg gttttggtgt tgtatgtaat caccgcacct
     6841 tcggcgacat cgccgccgcg ctgtactttg gcattaccgg ttaagactac ggtgctgcct
     6901 gcagatgaat aagcaacgtt gtttgcctgt ccgcgcaccg tgcctttgcc gccgtccaat
     6961 gtctggctga agcggactgg cgaaccttcc gccctcacgg attcgccgcc tttgccgccg
     7021 cgtgtaacat tgacgcgggc ggcggaaata ttgagcgtac cctgtctgat gacgacgttt
     7081 ccgctgaatg tggtgctttg gttggcttga tcgagcgaac cttggtcggc ctcaatctga
     7141 ataggctgcc tgctgtcgct ttgaagggca aaagcggggg acgccgaaaa aaatgctatt
     7201 aaaacaaata gcttacatat cttttgtatc ataaatcgtg gctttcactt tagatgagaa
     7261 gttcaacatg cctgttttgt ggtcgtaagt catgccgccc gcctgaccgt gcgatgcacc
     7321 atattggaaa ctgacaggcg tatcggtttg ggcatattga gattcggtat cgacgtgcag
     7381 cttttcggct tcaactttac ccgcctgccg tttgccgtcg gcggttttgg tcagcacaac
     7441 gttgttttta aaaagaacct gtttgttttc ggtatggtaa acggcttcat cgctgccgac
     7501 ttcgtacaac aacctgcctt cttggaagaa cacgagatgc ggtgagtcga aatgaatgtc
     7561 gctgctttcg ggaaactgtt tcgcgccctt cgaactcaaa tgttctttca agtatccctg
     7621 ttcgtcaaac cgcctgccat ccaatccgtc cattgtgtat tgcggttcgt cgggattgag
     7681 cctgacttct tcaatctcga cttcgctgat gcgtcccaac catgccgaca ggctgcccaa
     7741 ggcaaccgcc aatatcaatg ggaacgcaat tccgtaccgc catcttactt tcatttgatg
     7801 tactcgttca aagccgcgcc caaagtccct tgcgcctgca taatcaggtc gcacacttcg
     7861 cgcaccgcgc ccgcaccgcc ggggcgtttg gcaatatagg cggcgtgttg cagcgtaaac
     7921 caatgcgcgt cggggacggc aaccggcaat ccgcagcgta ccataacggg caaatcgacc
     7981 acgtcgtcgc cgacaaaggc acactcggct tcttccacgc cgacctgagc gcgcagctct
     8041 tcatacgcgg cgcgtttgtc cgaaatgcct ttgaagtagt aatttatgcc caactgtttg
     8101 acgcggatgc cgacggaggg cgcgtcccga cctgtgataa tcgcagtctg cacgccgctt
     8161 gcctgaagca ttttcagacc gtgtccgtcc agtgtgtgaa acgatttgat ttcttcgccg
     8221 ttatcgcgga taaagatgcg cccgtcggtc aaaacgccgt ccacatccag gatcaacagt
     8281 ttgattttgg cggcgcgcgc ctgtaattcg ggagaaattg cctgcatggg atttccttgt
     8341 tttcagacgg catatcttac cacaaatgcc gtctgaaacg gctgcaacg
//
LOCUS       DAWKOG010000084         8364 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.084, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000084 DAWKOG010000000
VERSION     DAWKOG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8364
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.084"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            311..1480
                     /gene="prpF"
                     /locus_tag="WJK66_001360"
     CDS             311..1480
                     /gene="prpF"
                     /locus_tag="WJK66_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HEZ7579067.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFAIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
     gene            1894..3093
                     /locus_tag="WJK66_001361"
     CDS             1894..3093
                     /locus_tag="WJK66_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ7579068.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSIVLSCLGERLTTPEAVI
                     TFSKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGLDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVACGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            3201..3821
                     /locus_tag="WJK66_001362"
     CDS             3201..3821
                     /locus_tag="WJK66_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEZ7579069.1"
                     /translation="MAQFFAVHPDNPQERLIKQAVEIVNKGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKDVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVIDMTDGTELVRQGCGDTAVFGL"
     gene            complement(3948..4391)
                     /locus_tag="WJK66_001363"
     CDS             complement(3948..4391)
                     /locus_tag="WJK66_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEZ7579070.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLAAPPDRINIGSVVRHLEPMQLVECMGENNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            4565..4933
                     /locus_tag="WJK66_001364"
     CDS             4565..4933
                     /locus_tag="WJK66_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ7579071.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFA
                     ELETHFREEEAKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            4978..6132
                     /locus_tag="WJK66_001365"
     CDS             4978..6132
                     /locus_tag="WJK66_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ7579072.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVIAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(6189..7061)
                     /locus_tag="WJK66_001366"
     CDS             complement(6189..7061)
                     /locus_tag="WJK66_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEZ7579073.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADSIGGADLVLIATPVATVPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHRCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALVEELWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            7146..7958
                     /locus_tag="WJK66_001367"
     CDS             7146..7958
                     /locus_tag="WJK66_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEZ7579074.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGVDWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGRDGKRTGLYHKMHLFGFSGLGERYAEADTIRAGGDVPHLSAEGVSVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGVVTADIDANRLNSVRNRLLALKYRVLDAV"
BASE COUNT         1803 a         2308 c         2416 g         1837 t
ORIGIN      
        1 gtactggttt ttgttaatcc actataaatt taatccacta tactgtaaat cgtcattccc
       61 gcgcaggcgg gaatccagaa agtggaattg aggaaacctt tttatccgat gagtttctgt
      121 gcggataaat ctggattccc gcctgcgcgg gaatgacggg gtttaataat ctgccgtatc
      181 acaacacagt agccgtagat tggggcgaac cccgacagtt tgcggaatca aacggctttg
      241 gtcggagtgg cagcctaatc cactataaaa atcgtgggca gagcccacgc tacataagga
      301 gaatctagaa atgccgcaaa ttaaaattcc cgccgtttac taccgtggcg gtacatcaaa
      361 aggcgtgttt ttcaaacgtt ccgacctgcc cgaggcggcg cgggaagcgg gaagcgcacg
      421 cgacaaaatc ctcttgcgcg tactcggcag cccggatccc tacggcaagc agatagacgg
      481 tttgggcaac gccagctcgt ccaccagcaa ggcggtgatt ttggacaagt ccgaacgcgc
      541 cgatcacgat gtcgattacc ttttcgggca agtttccatc gacaaacctt ttgtcgattg
      601 gagtggcaac tgcggcaacc tcaccgccgc cgtgggcgca tttgccatcg agcaaggctt
      661 ggtcgataaa ggcaagattc cttcagacgg catctgcaca gtcaaaatct ggcagaaaaa
      721 catcggcaaa accattattg cccatgtacc gatgcaaaac ggcgcagttt tggaaacagg
      781 cgattttgag ctcgacggcg taacgttccc ggcagccgaa gtacaaatcg aatttcttga
      841 tccagccgac ggcgaaggca gtatgttccc aaccggcaat ttggtcgatg aaattgatgt
      901 gccgaatata ggccgtttga aagccacgct catcaacgcg ggcattccga ccgttttcct
      961 gaatgccgcc gacttgggct acacgggcaa agagttgcaa gacgacatca acaacgatgc
     1021 cgcagctttg gaaaaattcg agaaaatccg cgcttacggt gcgctgaaaa tgggtctaat
     1081 cagcgacgta tccgaagctg ccgcccgcgc gcacacgccg aaagtcgcct tcgtcgcgcc
     1141 cgccgccgat tacaccgcct ccagtggcaa aaccgtgaat gccgccgaca tcgatttgct
     1201 ggtacgcgcc ctgagcatgg gcaaattgca ccacgcgatg atgggtaccg cctctgttgc
     1261 cattgcgacc gccgccgccg tgcccggtac gctggtcaac cttgccgcag gcggcggaac
     1321 gcgtaaagaa gtgcgcttcg ggcatccttc cggcacattg cgcgtcggtg cagccgccga
     1381 atgtcaggac ggacaatgga cggccaccaa agcggttatg agccgcagcg cacgcgtgat
     1441 gatggaaggt tgggtcaggg tgccggaaga ttgtttttaa attgacgtag catgggtttg
     1501 cccgcgagcc ataaacaggt cgtctgaaaa acaagtaaac atcaaatcac tgaccattcc
     1561 tttcccttgc cctgtggcgg aaggcggcaa atcacaagga agaacacgga aaccccgata
     1621 aaagacagct tcccgtatta ccgtcattcc cgcgcaggcg ggaatctaga cctgtcaata
     1681 tggaggattg gtaggggaaa acaggtttcg tgagttctac attctggatt cccgccacag
     1741 cctgtcctcg cgtaggcggg gacggaataa cgatagaaaa tgcggcatac gctttgccca
     1801 aagaggccgt ctgaaacacc ttgcgcctga tgtctgcctt tttcagacga ccccacacca
     1861 aaaaaacaac cacaaactac aaggagaaac atcatgtccg accaactcat cctcgttctg
     1921 aactgcggca gttcatcgct caaaggcgcc gttatcgacc gcaaaagcgg cagcatcgtc
     1981 ctaagctgcc tcggcgaacg cctgaccacg cccgaagccg tcattacgtt cagcaaagac
     2041 ggcaacaaac gccaagttcc cctgagcggc cgaaattgcc acgccggcgc ggtgggtatg
     2101 cttttgaacg aactggaaaa acacggtctg cacgaccgca tcaaagccat cggccaccgc
     2161 atcgcccacg gcggcgaaaa atacagcgag tctgttttga tcgaccaggc cgtaatggac
     2221 gaactcaatg cctgcattcc gcttgcgccg ctgcacaacc ccgccaacat cagcggcatc
     2281 ctcgccgcgc aggaacattt ccccggtctg cccaatgtcg gcgtgatgga tacttcgttc
     2341 caccaaacca tgccggagcg tgcctacact tatgccgtgc cgcgcgagtt gcgtaaaaaa
     2401 tacgctttcc gccgctacgg tttccacggc accagtatgc gttacgttgc ccctgaagcc
     2461 gcacgcatct tgggcaaacc tctggaagac atccgcatga ttattgccca cttaggcaac
     2521 ggcgcatcca ttaccgccat caaaaacggc aaatccgtcg ataccagtat gggtttcacg
     2581 ccgatcgaag gtttggtaat gggtacacgc tgcggcgaca tcgacccggg cgtatacagc
     2641 tatctgactt cccacgccgg tttggatgtt gcccaagtgg atgaaatgct gaacaaaaaa
     2701 tcaggtttgc tcggtatttc cgaactttcc aacgactgcc gcaccctcga aatcgccgcc
     2761 gacgaaggcc acgaaggcgc gcgcctcgcc ctcgaagtca tgacctaccg cctcgccaaa
     2821 tacatcgctt cgatggctgt ggcctgcggt ggcgttgacg cactcgtgtt caccggcggt
     2881 atcggcgaaa actcgcgtaa tatccgtgcc aaaaccgttt cctatcttga tttcttgggt
     2941 ctgcacatcg acaccaaagc caatatggaa aaacgctacg gcaattcggg tattatcagc
     3001 ccgaccgatt cttctccggc tgttttggtt gtcccgacca atgaagaact gatgattgcc
     3061 tgcgacactg ccgaacttgc cggcatcttg tagccaaaaa agggacgagt ccgcaaaaat
     3121 gccgtctgaa attttcagac ggcattttgt ttggcgtttg ggcgtacaat acggaaaaca
     3181 tgacgataag gaaacaaacc atggcacagt ttttcgccgt tcatcccgac aacccccaag
     3241 aacgcctcat caagcaggcg gtcgaaatcg tcaataaagg cggcgtggtc gtttatccga
     3301 ccgattcctg ttatgccttg ggttgcaaac tcggcgataa ggcggcgatg gaacgcatac
     3361 tctccatccg aaaaatcgat ttgaaacacc acctgaccct gatgtgcgcc gatttgagcg
     3421 agttgggcac atacgccaaa atcgacaacg tacagtttcg tcagcttaag gccgccacgc
     3481 ccggacctta tacctttatc ttgcaggcga cgaaggatgt gccggcgcgc acgctgcacc
     3541 cgaaacgcaa aaccatcggg ctgcgtattc ccgataatgc cattgcacaa gccctgctgg
     3601 gggaattggg cgaaccgctt ttaagctgca ccctgatgct gcccgaagac ggcgaaccat
     3661 tgaccgatcc ttatgaaatc cgcgagcgtt tggaacacgc cgtcgatttg gtgattgacg
     3721 gcggctggtg cggaaccgag ccgaccaccg tcatcgacat gaccgacggt acggaattgg
     3781 tgcgccaagg ttgcggcgat acggcggtgt tcggtttgta gggaaaccga tgccgtctga
     3841 agcatcggct gttcagacgg cattgcgcgc cttgccggcg gcagtccgaa atgccggcgc
     3901 gtatcgcgct cggtcggaat atccgtttga aacggcattt tgatgcatta ctgcaccgca
     3961 atcggaattc tcggttcgta gagcaggtcg taggtcggct tgttgagcag gtcttggagc
     4021 gtgaaaccgt ccagatacgt gaaaaacgac ttcatcgcgc cgccgagtat gcccgtcagc
     4081 cggcaggacg gtgtaatcag gcattcgttg ttctcgccca tgcactcgac cagctgcatc
     4141 ggttcgaggt ggcggacaac cgagccgatg ttgatgcggt cgggcggtgc ggcaagccgc
     4201 agaccgccgc cttttccgcg cacactgtgg aggaagccgc ctttgaccag cgcggtaacg
     4261 accttcatca gatggctttt ggaaatgccg taggttacgg cgatggtact gatgttgacc
     4321 agcgcatcgt cgttgatggc agtgtagata aggacgcgca gcccgtagtc cgtatgttgt
     4381 gtcaaataca tgattttctc ggtatggatt gttattctta tcggtacggt ttaaggttca
     4441 cggacaatac cttaatggtt gaaaccctgt ccgtcggggc ggtagaatgc agcctgtctg
     4501 cggcggtatg ccgtctgaaa catccgcgct accgtttgat aatttgttat tgtaactcaa
     4561 aatcatgaaa ccgttgaaac gacatcccgc ccttatcggg ctttcgcgtg accaccacca
     4621 ttcgctttcc ctgtgcgtgc gtctgttgcg gacgccggaa gaaaggcatc gggacgaact
     4681 cgaaccgcat tttgccgaat tggaaaccca ttttcgcgaa gaagaagcca agtttgcccc
     4741 aatttggcag aatgtcgccc ccgaattgaa acaacgtttc gagaaagacc acgcccgact
     4801 gcggcagatg atggcaagcc ccgaatacgg taacgcggcg tggaataccg cttttgccac
     4861 aaccctgcgc gaccacgcgc gctttgaaga acgcgagctg tttcccgccg ccgaaccgtt
     4921 tttgccggca tgattccgtt ttgcggcaaa tatattaatg ataaacaagg aacacacatg
     4981 aaatttacca agcaccccgt ctgggcaatg gcgttccgcc cattttattc gctggcggct
     5041 ctgtacggcg cattgtccgt attgctgtgg ggtttcggct acacgggaac gcacgagctg
     5101 tccggtttct attggcacgc gcatgagatg atttggggtt atgccggact ggtcgtcatc
     5161 gccttcctgc tgaccgccgt cgccacttgg acggggcagc cgcccacgcg gggcggcgtt
     5221 ctggtcggct tgactatctt ttggctggct gcgcggattg ccgcctttat cccgggttgg
     5281 ggtgcgtcgg caagcggcat actcggtacg ctgtttttct ggtacggcgc ggtgtgcatg
     5341 gctttgcccg ttatccgttc acagaatcaa cgcaactatg ttgccgtgtt cgcgctgttc
     5401 gtcttgggcg gcacgcatgc ggcgttccac gtccagctgc acaacggcaa cctaggcgga
     5461 ctcttgagcg gattgcagtc gggcttggtg atggtgtcgg gttttatcgg tctgattggt
     5521 acgcggatta tttcgttttt tacgtccaaa cggttgaatg tgccgcagat tcccagtccg
     5581 aaatgggtgg cgcaggcttc gctgtggctg cccatgctga ccgccatgct gatggcgcac
     5641 ggcgtgatgc cttggctgtc ggcggctttc gcgtttgcgg caggcgtgat ctttaccgtg
     5701 caggtgtacc gctggtggta taaacccgtg ttgaaagagc cgatgctgtg gattctgttt
     5761 gccggctatc tgtttaccgg attggggctg attgcggtcg gcgcgtctta tttcaaaccc
     5821 gctttcctca atctgggtgt gcatctgatc ggggtcggcg gtatcggcgt gctgactttg
     5881 ggcatgatgg cgcgtaccgc gctcggtcat acgggcaatc cgatttatcc gccgcccaaa
     5941 gccgttcccg ttgcgttttg gctgatgatg gcggcaaccg ccgtccgtat ggttgccgta
     6001 ttttcttccg gcactgccta cacgcacagc atacgcacct cttcggtttt gtttgcactc
     6061 gcgcttttgg tgtatgcgtg gaagtatatt ccttggctga ttcgtccgcg ttcggacggc
     6121 aggcccggtt gagacaaacc gccgcagatt tcgggtctgg gcggtttgct tttcagacgg
     6181 cagggcggtc agttgccgtc cagccagcgg tcgcgtgtgg ttttggcttc ttcaaaatag
     6241 cggtacaggg cttcgcggtc gtcggtggtc aggatgtttg ccaaaacgtc caactgtttg
     6301 cccaagcctt gaaccagttg cagcaggctg tctttgttgg caaggcagat gtccgcccac
     6361 acggcgggat gaccggaggc gatgcgggtg aagtcccgaa agcccgtggc ggcgaatttc
     6421 agatattcct gtccgtcggg gtggtcgaga atctggtgga cataggcgaa ggcggtcagg
     6481 tggggcatat gggagacggc ggcgaaaacc gcgtcgtggc gttgcgcgtc catcgtataa
     6541 atttccgcac cgaccgcgtg ccacagttct tctaccaagg caatgccgtc tgaatgttcg
     6601 ccgccgtgtg gcgtgatgat gagttttctg tggcggaaca gcccgaactg cgcggcttgc
     6661 gcaccgcttc tgtccgaacc ggcaatcggg tgggcggcga tgcagcggtg caggcggtcg
     6721 ggcagacagc ggcggaaggc ttcgatgacc gaagatttgg tgctgccgac atcggaaatc
     6781 caagtgtgtt ccggcaaaac ggggcgcagc gcggtcaaaa tggcgggaac ggtggcgacg
     6841 ggcgtggcaa tcagtaccaa gtccgcaccg ccgatgctgt ccgcgtcgat ggcaacggac
     6901 gcacggtcaa tcacgccgcg ttccaatgca cgttcgaggt tgtcgcggtc ggtgtcgata
     6961 ccggtaacgg tgcggacgag tccctgcctt ttgaggtcga gaacgaacga accgccgatc
     7021 agccctacac cgatgagggc aatatggttc aaaatgggca tttgtgtaaa cggttttcgc
     7081 aaagtaccgt catggtagcc tatcggcgga atatgccgca agttcggcag gaaaaaggag
     7141 aagaaatgga caaaatcaga gttgccgccg tgcagatggt gtcgggcgtg tcgccggaaa
     7201 ccaacgtcgc tgccatgaaa cgcctggtcg cacgggcggc ggagcagggt gtggattggg
     7261 tgctgctgcc cgaatattgg gtgctgatgg gcgcaaacga taccgacaaa ctcgcgcttg
     7321 ccgagccttt gggcggcgga cgctttcaga cggcattgag cgaaacggcg aaagaatgcg
     7381 gcgtggtgct gttcggcgga accgtgccgc tgcaaagccc cgaggcgggc aaggtcatga
     7441 atactttatt ggtgtacggg cgcgacggca aacggacggg gctgtaccac aaaatgcacc
     7501 tcttcggctt ttccggtttg ggcgaacgct atgccgaagc cgataccatc cgcgcgggcg
     7561 gggatgtgcc gcacttgtcg gcagaaggcg tgtcggtggc ggcgggcatt tgttacgatg
     7621 tccgctttcc cgaatttttc cgccgccagc tgccgtttga cgtattgatg ctgcccgctg
     7681 cgtttacgca cacgacgggc aaggcgcatt gggagctgct gctgcgcgcg cgtgccgtcg
     7741 aaaaccaatg ttacgtcgtg gcggcggcac agggcggttt gcacgaaaac ggacggcgca
     7801 cgttcggaca cagcatgatt gtcgatccgt ggggcgacgt gttggacgta ttgcccgaag
     7861 gcgaaggcgt tgttacggca gacatcgatg ccaatcgcct gaacagcgtc cgcaaccgcc
     7921 tgctcgcctt gaaataccgg gttttggatg ccgtctgaag gttcagacgg catcggtgcc
     7981 ggggaatcag cgggctgtag gatgggcttc agcctgccat ttccatcaat cccacatttc
     8041 taccgttttc atcgaatcca tcgaatccgc ccctttcgac gacccagtcc tacgcaactg
     8101 aaccgtcatt cccacggaag tgggaatcta gaatctcggg gtttcagtca tttccgatag
     8161 attcccgccg cgtcaggggt ctggattccc gcttgcgcgg gaatgacgaa tccatccata
     8221 cggaaacctg caccacgtca ttcccacgaa agtgggaatc tagaatctcg ggggtttcag
     8281 tcatttccga tagattcccg ccgcgtcgga ggtctggatt cccgcctgcg cgggaatgac
     8341 gggttttaag attgcggtat tgtc
//
LOCUS       DAWKOG010000085         8273 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.085, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000085 DAWKOG010000000
VERSION     DAWKOG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.085"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(27..653)
                     /locus_tag="WJK66_001368"
     CDS             complement(27..653)
                     /locus_tag="WJK66_001368"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEZ7579075.1"
                     /translation="MRKIRTKICGITTPEDALYAAHAGADALGLVFYPQSPRAVDIIK
                     AQKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFHRPYIK
                     AIRVQTASDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVDEAIRITGAEAIDVSGGVEASKGKKDPAKVAAFIATANRLSR"
     gene            complement(712..1203)
                     /gene="greB"
                     /locus_tag="WJK66_001369"
     CDS             complement(712..1203)
                     /gene="greB"
                     /locus_tag="WJK66_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEZ7579076.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLEAAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            complement(1303..2847)
                     /gene="purF"
                     /locus_tag="WJK66_001370"
     CDS             complement(1303..2847)
                     /gene="purF"
                     /locus_tag="WJK66_001370"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEZ7579077.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLVGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVHGAYGVVAMIAGYGMLAFRDPYGIRPLVLGSQTDSEGRKSYAVASESVA
                     FNALAYDLERDIRPGEAVFVGFDGTIIARQCSDKAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLKKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            complement(3056..3553)
                     /locus_tag="WJK66_001371"
     CDS             complement(3056..3553)
                     /locus_tag="WJK66_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEZ7579078.1"
                     /translation="MNNLPVADLLVFAIIAACIVISTMRGVIAEAGSMAAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFVIACLIQKILRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGILIITLLVMLASKTDLPDTEEWRQSYTLPFFVSLSEAVLNHSGGTAE
                     TPEDD"
     gene            complement(3546..4544)
                     /gene="ftsN"
                     /locus_tag="WJK66_001372"
     CDS             complement(3546..4544)
                     /gene="ftsN"
                     /locus_tag="WJK66_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ7579079.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINDRLEDSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKA
                     DKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
     gene            complement(4557..5831)
                     /gene="folC"
                     /locus_tag="WJK66_001373"
     CDS             complement(4557..5831)
                     /gene="folC"
                     /locus_tag="WJK66_001373"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEZ7579080.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVGEVKKRMKLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGYKTGTLTSPHLLRYNERIAINAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNTLAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DFDHQAFLGDTVEQVGFEKAGVFRGGKPAICGQNPAPASLLSHAEAIGAKLLMVQRDF
                     EFHALENIQWNYRFRPQHSDSPARNRNSLPFPALRGAYQLSNAACALTVLECLNDKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLEIVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHSIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            complement(5863..6309)
                     /locus_tag="WJK66_001374"
     CDS             complement(5863..6309)
                     /locus_tag="WJK66_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FolI"
                     /protein_id="HEZ7579081.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASLAVCLTWFYGRMMWFGLAA
                     LAASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKEKTAKAAALSGSSMVTPYALF
                     LQWDTGGKTVRHCITHDMADPESYRRLKVWVLWRQPKKTAETDTPS"
     gene            complement(6314..6673)
                     /locus_tag="WJK66_001375"
     CDS             complement(6314..6673)
                     /locus_tag="WJK66_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579082.1"
                     /translation="MYFVDRTAVVLKPTVRFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEADLNITPVFDNMM"
     gene            complement(6746..7474)
                     /locus_tag="WJK66_001376"
     CDS             complement(6746..7474)
                     /locus_tag="WJK66_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ7579083.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            complement(7647..>8273)
                     /gene="rsmA"
                     /locus_tag="WJK66_001377"
     CDS             complement(7647..>8273)
                     /gene="rsmA"
                     /locus_tag="WJK66_001377"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEZ7579084.1"
                     /translation="KKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGIA
                     GKKKIVGNLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVDRMVAAPKSNDYGRLGVM
                     LQYFFDMEMLIDVPPESFDPAPKVDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFHQR
                     RKTIRNNLKELAGDDDLQAVGINPQDRAEHIAPEKYVALSNYLAGKAV"
BASE COUNT         1622 a         2118 c         2406 g         2127 t
ORIGIN      
        1 tcattccggc ggcaattttt gttgctttaa cgggataggc ggttggcggt tgcgataaag
       61 gcggcaactt tggctgggtc ttttttgcct ttagacgctt ccacgccgcc ggatacatcg
      121 atcgcttccg ctccggtgat gcggatggct tcgtcgacgt tttcaggggt cagcccgccg
      181 gcaagcaccc acggtttgcc cgaatattcc gccaacagcg tccagtcgaa gcggtgtccg
      241 gtgccgccgt attccgaagg atggtaggca tcgaacagca gtgcctgagc gtcggggaag
      301 cggtcggcgg cgtttcggat gtctgatgcc gtctgaacac gaatggcctt gatatagggg
      361 cggtggaact ggcggcagaa tgcgtcgtct tcgtcgccgt ggaattggat gatgtgtatc
      421 ggtacttcgg caaggatgcg gcggatgttt tgcgcgcttt cgttgacgaa aagggcgaca
      481 acgctgacaa acggcggcag tgcggcggtg attttttgtg ctttaatgat gtcgacagcg
      541 cgggggcttt gggggtaaaa aaccagtccc aatgcgtctg cgccggcgtg ggcggcatac
      601 agtgcgtctt ccggtgtggt gatgccgcag attttggtgc ggattttcct cattcggtat
      661 tcctttattt gggaaacggc gcgtttgtgc cgtttcagac ggcattcccg atcagtcgat
      721 tttgatgtat tcgacagaaa ggatttcaat ttcctcacgc ccttccggcg tgtttaaaac
      781 cacttcgtcg ccttcacgcg ctttaatcag acaacgagcc agcggcgaaa tccaagaaat
      841 tttgttttgc gcggtatcga tttcatcgat gccgacgatt ttgacggttt gctcgcgccc
      901 gtcgccgcgc aacagaccga cggtcgcgcc gaaaaacact tggtcggtcg cttcgcgcaa
      961 ttcgggatca acgacgacgg cggcttccaa acgcttggtc aggaaacgga tgcggcggtc
     1021 gatttcgcgc atacggcgtt tgccgtaaag gtagtcgccg ttttcgctgc ggtcgccgtt
     1081 gcctgccgcc cagttgacga tttggacgat ttcggggcgt tctttattaa ccagttgata
     1141 aagctcgtct ttcaatgcct gccatccggc gggcgtaatg tagtttttgg tttcggtact
     1201 catattgtgt gcggatgaaa cgggaaatgt gatgccgata tgggaaatgc cgtctgaaaa
     1261 cccggcgttc ggatttcaga cggcatcgcg gtttgggaag ccttattctt cgtcgcccgc
     1321 atcgctgatg ctgatgctgt gttccatcct gctcgggtgg attttcagac cgccgcagcc
     1381 ggatttctcg gcagacaggc ggtcgaggta ggcatcatcg atgtcgccgg tctgataaat
     1441 gccgttgaaa caggacgaat cgaaggattc gattttcggg ttgagtgctt tgacgacggc
     1501 ttccaaatcg cccaagtctt gaaacacgat gccgtccgcg ccgatttcgg cggcgatttc
     1561 cgccgcgctg cgcccattgg caatcaactc ttcgcgcgtg ggcatatcga tgccgtatac
     1621 gttgggatag cgcacttcgg gcgcggcgga ggcgatatag actttgcgcg cgccagccgc
     1681 gcgtaccatt tcgacgattt cacggctggt cgtaccgcgc acgatggagt cgtccaccag
     1741 caacacgctt ttgcctgcaa attcggtttc catcgggctg agtttttggc gcacggattt
     1801 tttgcgcgtc gcctggccgg gcataataaa ggtgcggccg atatagcggt ttttaatcaa
     1861 accttcgcgg taaggctttt tgaggtggac agcaagctcc atcgcgctgg ggcggctggt
     1921 gtcggggatg ggcatcacga catcgatgcc gtccacgggc agctcgcgtt tgattttttc
     1981 cgccaaggac acgcccatat ccaagcgcga ctggtaaacg gacacgccgt caatcacgga
     2041 gtcgggacgg gcaaaataga cgtattcaaa aaggcagggg ctgagtttgg ccttatcgct
     2101 gcattgtcgg gcgatgattg tgccgtcaaa gccgacaaat accgcttcgc ccggccggat
     2161 atcgcgttcc aaatcgtagg caagcgcgtt gaaggcgacg gattcggagg cgacggcata
     2221 ggatttcctg ccttcgctgt cggtttgcga acccaatacc agcgggcgga tgccgtaagg
     2281 gtcgcggaag gcaagcatac cgtagcccgc aatcatggca accacaccgt atgcgccgtg
     2341 caccaggcgg tggacttcgg caacggcgtt gaaaatattg tcggcattga gccggtgcgg
     2401 gtcggcgttt ttagagactt cgcggcgcaa ttcgtgcgcg aatacgttga gcaagacttc
     2461 ggaatcggag ctggtgttga cgtggcgcag gtgtttgttg cacacgtttt cataaagttc
     2521 ggccgtgttg gtgaggttgc cgttgtgcgc caaaacgatg ccgaacggcg agctgacgta
     2581 gaaaggctgc gcctccgcgc tgctgcccgc gttgcccgca gtaggataac ggacgtgggc
     2641 gatgccggcg ttgccgacca aatcgcgcat attgcgtgtg cggaacactt cgcgcaccat
     2701 gcctttgcct ttgtgcatat ggaaggtacc gccttccgcc gttgcaatgc ccgccgcatc
     2761 ctgccccctg tgctgcaaca tctgcaaacc gtcgtacaga agctggttca cgggttcatg
     2821 actgaccaaa cctaatacgc cgcacatatc gtcttctccg attcgaggtt taagggtaaa
     2881 acggaattat aaagcaaacg gtggtttttt gcctgaattg ttgacaatat ttgagtgaag
     2941 gacagatagg tgggtttatg gaggataaga tttaatctgt gatatggctg aggaaaggaa
     3001 aaaaacattt cagacggcat aaaagagaat gccgtctgaa atatccgtat ggcaatcaat
     3061 cgtcttccgg agtttccgcc gtgccgccgc tatggttcaa cacggcttcg gaaagcgata
     3121 cgaaaaacgg cagtgtgtaa gattgccgcc attcttcggt atcgggcagg tcggtttttg
     3181 aagcgagcat gaccagcagg gtaataatca aaatgccttt caatgcaccg aatacgccgc
     3241 ccaaaatgcg gttggcaaag cccaaaccga ccgccgaaac tgccccggtc agcagcgagc
     3301 ggagtatttt ctggatcaga caggcaatga cgaataggga aatgaacgac agagccaatg
     3361 caaacaggcg gggttggaac gaggcaaagg cgaggtcggc gaagggtgcg gcaaagagtt
     3421 tggcaaaaaa gaaggaaacc acccatgccg ccatcgaacc tgcctccgca atcacgccgc
     3481 gcatcgtgga aatgacgatg caggcggcga tgatggcgaa gacgaggagg tcggcaacgg
     3541 ggaggttatt cattcgttac ctgaccggcg ataccgtgta cacgcaattt gttcaaatcg
     3601 cgttcggcat cccttgcgtt tttatagttg cttgatttga cgcggtaaac tttgccgttg
     3661 tcggtcataa tttcggtgat ggtcgaatcg atacccgccg ccttcatttt gcgctggagg
     3721 cttaaggcgc gttctttttc ggcataacct gcctgaatgg cggctttttt accggatttt
     3781 tccttctcgg cggttttggt cttgtccgct ttgtcggttt tttgtgccgt ttcgtgtttt
     3841 ttgccgtccg aacggtcttt ttcggctgtt tttttgcttt cagccttgtc ggcttttttc
     3901 gcttctttta ccgcgctgtc ggattttgcc gtatccggtt tggttttttc ggcggcagtt
     3961 ttcggtttgt cggcaacttt ttcggcggtt ttggtttctt tggctttggg cttggctttg
     4021 gcagtgcgtt ccgctttttg cggttttgtt tcggcagtgc gtttcggttt ttcaaccgct
     4081 accgtatccg tactgtcggc agttgccggc actttttcgg cagcgcgttg ttttgcctgc
     4141 ttcggtgcgg ttttggcggt ttctgcctgt tgcagtttct cggatgcttc caaacctttg
     4201 atgttgctgt cttcgaggcg gtcgttaatc agcaccagcg gcgcgcctac gttttcaggc
     4261 tcgctgattt cgctgtcggc ggcagaaggc ttgtcttcgc ctgccaagtc ctgcggtttg
     4321 tcggcggcgg atttcaaggc aggggtttgt gccgcacctg ccgctttgtt ttctacgccg
     4381 cttgtttcgc cggcagtctg ttcggcaggg ccggaactga gggcggctgc cagcaggatg
     4441 caggaggcgg caaccaggca acttgccgtt acgaggcggc ggcggttgcg ccgtttgagt
     4501 tgttcgtaac cgctcaggac ttcgttttgt ttgttttcgg acatagaagt ttccttttaa
     4561 agtaccgaca tgacatcggc aacggtatga aatgagccga aaacgacgat tctgtcgttc
     4621 tcgcccgctt ttgaggctgc cgcccggtaa gcttcgcgga cggcggcgaa tgtctgtatg
     4681 ttttcgatgc tgtgttcgtg cagtttgttt tgcaggctct cgagcgtcat gccgcgcggt
     4741 acgtccaacg gtgcgatata ccactcgtca aactggtctt taacgatttc caacacgccg
     4801 tctatgtctt tgtcggacag catactgaat acggcggtgc gtttttgcgc aaacgccaaa
     4861 ttaatcaggc tgcggcgcag ggcgcgggca gcgtggggat tgtgtccgac gtccaaaacg
     4921 gtcagcggcc ggccgggcag gacttggaag cgtccgggat tttcaaccag cagcaatccg
     4981 cgtttgattg caccgatgtc caccggcaat ttgtcgttca aacattccaa cacggtcagc
     5041 gcgcaggcgg cgttggaaag ttggtatgcg ccgcgcaatg cggggaaggg cagggaatta
     5101 cggttgcgag cggggctgtc tgaatgctgc ggtcggaagc ggtagttcca ttggatgttt
     5161 tccagcgcgt gaaactcgaa atcgcgctgc accatcagca gtttcgcgcc tatggcttcg
     5221 gcgtgtgaaa gcaacgatgc gggcgcggga ttttgaccgc agattgcggg tttgccgccg
     5281 cggaacacgc ctgctttttc aaagccgacc tgctcgaccg tatcgcccaa aaacgcctga
     5341 tggtcaaaat ctacactagt aaccaccgcg caatcgccgt caaatgcgtt gaccgcgtcc
     5401 aagcgtccgc ccaagccgac ttccaatatc atcacgtcaa cctgttcgcg catgaagatg
     5461 tcgacggccg ccaacgtatt gaattcaaaa taagtcagcg atatttcgcc gcgcgccgct
     5521 tcgatgcgct cgaaagaggc aataatcgta tcgtccgaaa cgggttcggc gttgatggcg
     5581 atgcgttcgt tgtaacgcaa taaatggggg ctggttaatg tgccggtttt gtagcccgcc
     5641 tgtttgtaaa tctgtgtcag gtaggcgcag accgaacctt tgccgttagt tcccgcgaca
     5701 acgacgacgg ggcattgcgg cgtgagcttc atccgttttt tcacttcgcc tacgcgttcc
     5761 aagcccatat cgatcaaacc gccgctgtgg gcggtttcca aatgggagag ccagtcttgc
     5821 agtgttttca ttgagtgttc cgtgttcaaa tgccgtctga aatcagcttg gtgtatcggt
     5881 ttcggcggtt tttttcggct gccgccaaag cacccagact ttcagccgcc ggtaggattc
     5941 cgggtccgcc atatcgtggg tgatgcagtg gcggacggtt ttgccgccgg tgtcccattg
     6001 taagaataag gcataaggcg taaccatact gctgcccgac agtgccgccg cctttgctgt
     6061 tttttctttg ccggatacga tttccgcctg tccgtcgcgg tctatggtaa tggcggttat
     6121 ggcatggcgg tgtttcagat tcgttatcct gagcgagtat gcgtaacttg ccgccaaagc
     6181 cgccaaaccg aaccacatca tccggccgta aaaccaggtc aggcagacgg caagggaagc
     6241 cgagtggagc gatacggtca ggatgttcag gatgcgggac ggcctcaatg ctgtctgaaa
     6301 ggcgcgcacg gcgttacatc atattgtcga acacgggcgt gatgttcaaa tccgcttctt
     6361 ccatatccaa caccatatcg tggatttcga tgtcgaaaaa ttcccaaaac gccttcagcc
     6421 ccatatcttg cggccattta tccttgtcga tgtcccagcc ggcgagttcg gcttcaaaaa
     6481 tctggcggta gcgttcgtcg aaataggaaa cgacggcttc cggttcgtcg aactgcggaa
     6541 cgaggaagac ggagcagttg gcacgaagct gctctatggt caggtcgggc atattttcgt
     6601 cggtgctttt gagccattcc aaaaagcgga cggtcggctt gaggacgacg gcggtgcggt
     6661 caacaaaata catggttttc tttctcaatc atcttgcggt gtcgggctat gccgtctgaa
     6721 cgttcggttt tcagacggta tagcatcagt gggtcatgac ctgttgcagg aactgcttgg
     6781 cgcgttcgtg tttcgggttg gtgaaaaacg cttcgggcgt ttcgtcttcg aggatttgcc
     6841 ctttatcgac gaaaatcacg cggtcggcaa cttcgcgggc aaaccccatt tcgtgggtta
     6901 cgcacatcat ggtcatgccg ctttctgcca agtctttcat cactttcaac acttcgccga
     6961 ccatttcggg gtcgagtgcg gaggtcggtt cgtcaaacaa cattacgcgc ggttccatcg
     7021 ccaaaccgcg tgcaatcgcc acgcgctgct gctggccgcc ggaaagttgg gacggaaaag
     7081 cgtctttttt gtgcgccagt ccgacgcgtt ccaaaagttc catcgctttt ttctccgcct
     7141 gttccgcatt ttgaccttta accttcatcg gtgcgagggt gatgttttcc aatacggtca
     7201 ggtgggggta gaggttgaaa ctttgaaaca caaagccgac ttcttcgcgg attttgttca
     7261 aatcggtttt tgggtcggca acgttgacac cgtccaccca aatctcgccg ctttcgatgc
     7321 tttcaagctg gttgacggtg cggatgagtg tggatttgcc gctgcccgaa ggtccgcaga
     7381 cgacgaccac ttcgcctttt ttgatttcca agttcacgcc gttgatgacg tgcaggtctt
     7441 tgaaatgttt gtgtacgttt ttgaatttga tcatgtcttt tctttcagac ggcatttgcc
     7501 gttgcaggtt gtcgttacgg gagctccata tgatgaagcg tgtagcgtct gccgtcaaaa
     7561 aaacggtcgt tcggattggt caggcaggct gcaagcggca gtatcagggg taaaagcagg
     7621 tatttcgtca tcggcttact cccttttcag acggccttgc ccgccagata attgctcaac
     7681 gccacatatt tctccggcgc gatatgttcg gcgcggtctt gcggattgat gccgactgcc
     7741 tgcaaatcat cgtcgcctgc aagctctttc agattgttgc gtatggtttt gcggcgttgg
     7801 tggaaggcga gtttcacgag tttggcgaaa tgctcgaaat cgtccgcctt gccgatacgg
     7861 tgtttcaccg gaatcatgcg gacgacggcc gaatccactt tcggcgcggg gtcgaacgat
     7921 tcgggcggca cgtcaatcag catttccata tcgaagaaat attgcagcat cacgcccaag
     7981 cggccgtagt cgttgctttt cggcgcggca accatacgat cgaccacttc tttttgcagc
     8041 ataaagtgca tatcgacgac atcgtccgcc acctccgcca gcttgaacaa aagcggcgtg
     8101 gaaatgttgt agggcaggtt gccgacgatt ttctttttgc ctgcgatgcc gttgaaatca
     8161 aactgcaata catcgccttc gtgaatcacc agtttatccg caaacggcag cgttttcaga
     8221 cggcatacga tgtcgcggtc gatttcgaca acgtgcaggc ggttcagctt ttt
//
LOCUS       DAWKOG010000086         8263 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.086, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000086 DAWKOG010000000
VERSION     DAWKOG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8263
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.086"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(216..944)
                     /gene="rmpM"
                     /locus_tag="WJK66_001378"
     CDS             complement(216..944)
                     /gene="rmpM"
                     /locus_tag="WJK66_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HEZ7579085.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPAPVVVVEQAPQYVDETISLSAK
                     TLFGFDKDSLRAEAQDNLKVLAQRLGQTNIQSVRVEGHTDFMGSDKYNQALSERRAYV
                     VANNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPD
                     RRVDVKIRSIVTRQVVPAHNHHQH"
     gene            1308..2258
                     /locus_tag="WJK66_001379"
     CDS             1308..2258
                     /locus_tag="WJK66_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEZ7579086.1"
                     /translation="MKLQQLKYALEVYQHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIARMVTSGEADLAIVTERIDDHPELGK
                     LPCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(2371..3105)
                     /gene="fnr"
                     /locus_tag="WJK66_001380"
     CDS             complement(2371..3105)
                     /gene="fnr"
                     /locus_tag="WJK66_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEZ7579087.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            3310..4731
                     /gene="hemN"
                     /locus_tag="WJK66_001381"
     CDS             3310..4731
                     /gene="hemN"
                     /locus_tag="WJK66_001381"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEZ7579088.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTASV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            complement(5084..6658)
                     /locus_tag="WJK66_001382"
     CDS             complement(5084..6658)
                     /locus_tag="WJK66_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEZ7579089.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
     gene            complement(6640..6876)
                     /locus_tag="WJK66_001383"
     CDS             complement(6640..6876)
                     /locus_tag="WJK66_001383"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579090.1"
                     /translation="MEKYYNAPATSSSRSVIQTVSGSSFAFGQVWGYSDAVQKEGCVC
                     HIPKRQFDRRQQYSVGNTHDFHHIKGRLPWLKSK"
     gene            6983..8083
                     /locus_tag="WJK66_001384"
     CDS             6983..8083
                     /locus_tag="WJK66_001384"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEZ7579091.1"
                     /translation="METQLYIGIMSGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPDR
                     LRRQLLDLQDTGADELHRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAERTQIFTVGDFRSRDLAAGGQGAPLVPAFHKALFRDDKE
                     TRVVLNIGGIANISVLPSDAPAFGFDTGPGNMLMDAWMQAHWQLPYDKNGAKAAQGNI
                     LPQLLDRLLAHPYFAQPHPKSTGRELFALNWLETYLGGGENRYDVLRTLSRFTAQTVF
                     DAVSHAAADARQMYICGGGIRNPVLMADLAEYFGTRVSLHSTAELKLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
     gene            8202..>8263
                     /locus_tag="WJK66_001385"
     CDS             8202..>8263
                     /locus_tag="WJK66_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ7579092.1"
                     /translation="MKILLLLIPLVLTACGTLTG"
BASE COUNT         2084 a         2325 c         2015 g         1839 t
ORIGIN      
        1 acggcaatgg gacacaaacc tgaatctgaa tctgaatctg aacctgcacc ccgattctga
       61 cgatagccgt ttcagaaaac cacccaaaaa aacaaacccc gcaaaagcgg gggtttgttt
      121 cacatcatcg atgccgaccg acgtttcacg aatattgcaa cagtacctgg tacaaaatcc
      181 actaacctca tgcatcggca agatattgcc tagccttagt gttggtgatg attgtgtgcc
      241 ggcacaacct gacgggttac gatgctgcgg attttcacat ccacacggcg gtcaggttcg
      301 atacatgcaa tcagagcctc acgttttttg gctttagaga ctttcgcacc cagtttggca
      361 acttcggctt cacaaacttg agtcatttgc gcttgagatt cgcccaagcc gacagcagaa
      421 attctagaaa caggtacgcc gttgctgacc aggttgtttg ccactacgta tgcgcggcgt
      481 tcggacaggg cctgattgta tttgtcagaa cccataaagt cggtatggcc ttcgacgcgg
      541 acagattgga tattggtttg acccaggcgt tgcgccaata ctttcaggtt gtcttgagct
      601 tcggcgcgca atgaatcctt atcgaaaccg aacagggttt tggcagacag ggaaatggtt
      661 tcatcaacat attgcggagc ctgctccaca acgacgacag gcgcgggtgc gggttcgggt
      721 tctggctcgg gttcgggggc agcaaccgca tcgccgcatt ctacgcgacc ttggcttgct
      781 ttatcaaagt aggcgttttt ccagcattcg ccatagttgt tgcgtacgat ttcgttcgac
      841 tggccgctta cggtgtaacc ctgaacggac gcctcgcccg caacagcagt gccggaagcg
      901 agcaatgcaa cgaataatgc gcttaatttc agctgtttgg tcattttatt ccctcattaa
      961 atttgtacag cagatacaga agcaactgct gaattgccaa catacaggcc gctgtagaaa
     1021 catggcggca aatcggatat ttcagtctcc actataaaga agcaggggga ggactgtcaa
     1081 tactcaacgc ctgaaaacaa ccgtaaaaca gcctgtttcc tgatagtcta tcatgccgaa
     1141 aaatcaattc cgtgtcatta ctttaggggg attttttcca tatcgcacga ttgccgttgc
     1201 acaaaaacaa caaacacaac ggcaaagccc cataccgtac cggcaagaaa atatgataaa
     1261 ttataaacaa tgttcagcca cccgacacag acccacaccg acccgccatg aaattacaac
     1321 aattgaaata cgccttagaa gtttaccagc acaacctgaa cgtttccgaa gcggccgaag
     1381 ccttgtttac ttcgcaaccc ggcatctcca aacaaatcaa attgctggaa gaagaaatcg
     1441 gcattcagat ttttatccgc agcggcaagc gcgtggtttc ggtctcgcag ccgggcaagg
     1501 tggttttgga tattgcggaa cgtattttac gcgatgtaca gaacattaaa aatatcggca
     1561 gcgagtttac cggacaggac agcggttcgc tgacggttgc cacgacgcat actcaggcac
     1621 gctatgccct acccttgatt gttgccgatt ttgtgaaacg ctatccgaaa gtcaacctga
     1681 ccatcaaaca ggggagccct gccgccatcg cacgtatggt tacctcaggc gaggcggact
     1741 tggcgattgt tacggaacgg atagacgacc atcccgaact gggaaaactt ccctgctatg
     1801 actggactca tgcggttatc gtaccgaacg accacccctt gctcgaatgc agaaaccccc
     1861 tccgcattga agatttggcg aggtttccgc tgattactta tgaatttgca ttcaatgcgg
     1921 gcagcagcat cgcgcgggca ttttccaaag cccgtttgga acagccggat gtcgcattgg
     1981 cggcggcgga tacggacgta ttgaagactt atgtgcgctt gggtttgggc gtgggactga
     2041 tggcgaaaat ggcgtacaac ccggatacgg acggcgattt gcagcttgtg gatgcggcac
     2101 acctgttcga gccgtcgccg acgtggattg ctttgcgcag cgatacttat ttgcgcggat
     2161 atgcctacga ctttatccaa gcgtttgcgc cgcacctgac acgcgagaag gtggatagga
     2221 ttctgtacac gcccatcagc gaggattttt cgatttaggc ggctgccggt tttcagacgg
     2281 cactttgcgg cagatacaac aaacaggaca gatgttttcg tctgccctgt gtttattgag
     2341 aatgctgtct gaaatgttcg tacgggttaa tcaaatggcg tgcgagcagc cggacaccat
     2401 ttttttcaac acctgcagat tgaggatttt gatgtgctta tgctcgacgg aaatcaatcc
     2461 ttcctgatgg aacttggata atgtgcggct gacggtttca agtttcagcc cgagataact
     2521 gccgatttct tcgcgggaca ttcttaagat gaagtcgttg gcagcaaaac ctcgggaata
     2581 aaggcgttgg gaaaggttca gcaggaaggc ggcaatccgc tcttcggcgc gcatattgcc
     2641 caacagcagc ataacacctt ggtcgcgcac gatttcacgg ctcatcatgc ggaagaagtg
     2701 cgtacgcagg ctggggatgt tttgccccag ttcttcgatg tgggtaaacg gcagttcgca
     2761 cacttcgctg tcttccaagg cgaccgcgtc gcaactgtgc acatgggaac agatgccgtc
     2821 catgccgatg agttcgcccg acataaagaa acccgttacc tgatcgcggc cgtcctgact
     2881 ggcgacggtt gttttgaaga agcccgaacg gatggcaaag agcgaggtaa aggcttcgcc
     2941 gacacagaac aggtattcgc cctttttcag gcggcggctt tgacggatga cggcatcgag
     3001 ttggctgagc tcgttgggca gcagcccgac aggcaggcag agttcccgca aagaacagga
     3061 agaacacagc gttttcatct gatgtgtagt attatgcgaa gccataccgt acctttttgt
     3121 gcgctttgcc ccatcatgat tatagtggat taaatttaaa ccagtacggc gttgcctcgc
     3181 cttgtcctta tttaaattta atccactata tgtgcttatt gacacatatc aagacaggtt
     3241 tatcatactg tggcattcta ccaaactatc caaaatttga ctaacatcat aaaaccgcac
     3301 acacacacca tgaaaatcat tcagatacag aacaatcaca atgtaaacga tgaccgcccc
     3361 gagtttgacc gcgcgctgat tgccagcctg cccgccagcg gcccgcgcta cacttcctac
     3421 cctaccgccg accgtttcca tgacggtttc cgcgaaggcg aatatatcaa agctttacat
     3481 ttgcgcggta tgggcgcgtt aaacaaaccg ctttcccttt acattcacat tccgttctgc
     3541 aacaccatct gctactactg cggctgcaac aaaatcatca ccaaagacaa aagccgcgcc
     3601 gatgcctaca tcgaatatct tgaaaaagaa atggaactgc tcgctccaca tctgaacgga
     3661 cggcaccagc ttgcccaact gcacttcggc ggcggcacgc cgaccttttt gagcgacgaa
     3721 cagatcgaac gtgtcttccg catgatacgc aaacatttcg agttaatccc caccggcgaa
     3781 tactccatcg aaatcgaccc gcgcaaagtc agccgcgaca ccgtcctcat gctcggcaga
     3841 ctcggcttca accgcatgag catcggcatt caggatttcg accccaaagt gcaggcggcg
     3901 gtcaaccgca tccaaagtta cgaagaaacc aaagaagtca tcgatgcggc gcgcgaagcg
     3961 gggttcaaat ccgtcagcgt cgatttgatt tacggcctgc cgcaccagac ttcggaaagc
     4021 atcaaaacca ccatcgatac cgttttgtcg ctcgatcccg accgcctcgc cctttatcac
     4081 tacgcccacc tgccgcacgt gttcaaaccg caacgccgca tcgataccgc ctccgtcccc
     4141 gacagcgaag agaagctcga tatgctgcaa tactgcgtcc aaaccctaac cgaacgcggc
     4201 tacgtcttca tcggcatgga tcatttcgcc aaacctgacg acgaactctc catcgccctc
     4261 aaagaaggct tcctccagcg caacttccaa ggctattcga cctacgcgga ttgcgatttg
     4321 gtcgccatcg gcgtgtcgtc catcggcaaa atcggcagca cctattccca aaacgaacgc
     4381 gacatcgatg cctactatgc cgccatcgac gaaggcagac tgcccatcat gcgcggttat
     4441 cagctcaatc aggacgacat cctgcgccgc aacatcattc aggatttgat gtgccgtttc
     4501 gcgctcgact atcggattta cgaaagtatg ttcggcatcc cgttcgaccg ctacttcaaa
     4561 gacgaactgg cggatttgga aaaactcgcc ggtttgggat tggtgcgcct gaacagccac
     4621 ggactgaccg ttaccccgaa aggacgcttc ctcatccgca acatcgccat ggttttcgat
     4681 taccacctgc gccataaaga aaccaaggcg aaatattcgc aaacggtgtg attgtggcta
     4741 acgtacaaat gccgtctgaa aggctttttc agacggcatt ttgctgccgg caggataagt
     4801 gttttcaaga acaggcggcg gcatatcata acgttccgca cctttgtgtc cgaccgttcc
     4861 gaaaccaaga tatagtggat taacaaaaac cagtacagcg ttgcctcgcc ttgctgtact
     4921 atctgtactg tctgcggctt cgtcgccttg tcctgatttt tgttaatcac tataccaaac
     4981 ggcatatccc gacagaacag attgtgcata aggcacaagc ccgcacattc catcaacaaa
     5041 atgccgtctg aacacgggtt cagacggcat cagtatttta caatcagaat actgcctgta
     5101 aaaccaagta gcagacaacc gacaatacgg cggcggcagg cagggtaatg acccacgcca
     5161 aaccgatggg cttcatcagt ttccagttgg cattgcggtt gaccagaccg atgccgagta
     5221 ccgcgccgac caagatatgc gtactggaca cgggcagccc catcagcgac gcgcccatca
     5281 cgacggatgc ggcggacagt tcggcggtaa aacccgaagc aggatgcatt tccgccaaac
     5341 tcgtaccgac ggttttaatc acctctttac cgacaaacca caaaccgaca atcagcgcga
     5401 taccgaaagt cagcatcgca atcgggggga cgacattttg cgcggcaacg ctgttggtac
     5461 gcaaaacatc cataatcgcg gcaaacggac cgatggcgtt ggcgatatcg ttcgccccgt
     5521 ggctgaatgc gaagccgcag gcggtaaaga cctgcatcca tgaaaacatc tgaaaggtcg
     5581 atttgcccaa gtctttacgc ttgaggcttt tggcaaacac aaacgtcccc atccacaccg
     5641 ccgcgcctat cataaagatg gtcaggaagc tgttgacgtt gctcatcccc aaatgcaggt
     5701 ttttcaagcc cttgaaaatc agcatagcgg aaatcatcat cgcgccgaac gaagcgataa
     5761 agggaatcca agaatgcagt gccttgtagg aatcgacatt gtttttacgg ttgtcgaacg
     5821 cataaagacc gcggtaatac tccgattgca gctcttgcgg atcgaattcg ggttcgtcgt
     5881 aaatttgcgc gtcgtgcgcc attttggtgg cgtactcgac tttttcggct tcggacaaac
     5941 cctcgaaaaa caggcggtgc cgttctttat aggccttttt ttcctgcttg atgcccttga
     6001 gcgtgccttc cgcccaagcg ttgtaatcta agacgttttt cttgacgcgc gaaaacagga
     6061 aataggacac cgcgccgccc aacacgggcg acaataccca agaaacacca ataccgccca
     6121 gcttgcccca acgtatcaaa tcgcccgatg cggcatcgtt cattaccgcc atacataccg
     6181 cgctaccgac aatgccgccg ataatggaat gggtggtaga taccggaagc ccttttttcg
     6241 aggcaaacaa cagccacaac gccgccgcca aaagcgcgga catcataata aacacaaact
     6301 gtatgggttc gaaatcaaca cccttcaaat cgacgatgcc tttgcgtatg gtattggtta
     6361 cctcgccgcc cgcgatgacc gcgccgctga cctcaaatac cgccgcaatc agcaaagcct
     6421 gcgggatggt cagcgtaccc gcaccgacgc tggtgccgaa agaattggca acatcgttgc
     6481 cgccgatgtt gaacgccata aacacgccga acatcgtggc aataatgaat aaggcggcgt
     6541 aattgtaatg ggtataaccc aagccccaat aaataaaata gccgactgta cctaccagca
     6601 tggcggcaaa gacggcgttg atggttttaa catttgcgct catttggatt tgagccatgg
     6661 caatcttcct ttaatgtggt ggaaatcatg agtgttgccg acactgtact gctgcctccg
     6721 gtcgaactgc cgtttcggga tatggcagac gcatccttcc ttctgaaccg catccgaata
     6781 cccccaaacc tgaccgaatg caaaactgct gccggaaacg gtttgtatga cacttctact
     6841 actggatgtt gcgggcgcat tataatattt ttccatccgt cttgaaacat ttatttacac
     6901 tttatttaca ctgcggcggc aaatcggtat acgagcgtca atacacgtta aaatggcgtt
     6961 ttgcaccagt ttgggagtga tgatggaaac acagctttac atcggcatca tgtcgggaac
     7021 cagcatggac ggggcggatg ccgtactgat acggatggac ggcggcaaat ggctgggcgc
     7081 ggaagggcac gcctttaccc cctatcccga caggctgcgc cgccaattgc tggatttgca
     7141 ggacacaggc gcagacgaac tgcaccgcag caggattttg tcgcaagaac tcagccgcct
     7201 gtacgcgcaa accgccgccg aactgctgtg cagtcaaaac ctcgcgccgt ccgacattac
     7261 cgccctcggc tgccacgggc aaaccgtccg acacgcgccg gaacacggtt acagcataca
     7321 gcttgccgat ttgccgctgc tggcggaacg gactcagatt tttaccgtcg gcgacttccg
     7381 cagccgcgac cttgcggccg gcggacaagg tgcgccgctc gtccccgcct ttcacaaagc
     7441 cctgttccgc gatgacaaag aaacacgcgt ggtactgaac atcggcggga ttgccaacat
     7501 cagcgtactc ccctccgacg cacccgcctt cggcttcgac acagggccgg gcaatatgct
     7561 gatggacgcg tggatgcagg cacactggca gcttccttac gacaaaaacg gtgcaaaggc
     7621 ggcacaaggc aacatattgc cgcaactgct cgacaggctg ctcgcccacc cgtatttcgc
     7681 acaaccccac cctaaaagca cggggcgcga actgtttgcc ctaaattggc tcgaaaccta
     7741 ccttggcggc ggcgaaaacc gatacgacgt attgcggacg ctttcccgtt ttaccgcgca
     7801 aaccgttttc gacgccgtct cacacgcagc ggcagatgcc cgtcaaatgt acatttgcgg
     7861 cggcggcatc cgcaatcctg ttttaatggc ggatttggcg gaatatttcg gcacacgcgt
     7921 ttccctgcac agcaccgccg aactgaagct cgatccgcaa tgggtggaag cagccgcatt
     7981 tgcgtggttg gcggcgtgtt ggattaatcg catccccggt agtccgcaca aagcaaccgg
     8041 cgcatccaaa ccgtgtattc tgggcgcggg atattattat tgagcgcaaa taccgtctga
     8101 aagccatttc cgccgctcag acggcatttt cgcccctttc gtttacaaac ccttaaaatc
     8161 catttacact caaacccgtt caaaacaaaa caaaccctgc catgaaaatc ctgctcctcc
     8221 tcatccccct cgtcctaacc gcctgcggca cactgaccgg cat
//
LOCUS       DAWKOG010000087         8156 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.087, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000087 DAWKOG010000000
VERSION     DAWKOG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8156
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.087"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(84..1187)
                     /locus_tag="WJK66_001386"
     CDS             complement(84..1187)
                     /locus_tag="WJK66_001386"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEZ7579093.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGADDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQSGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(1267..2823)
                     /gene="dnaA"
                     /locus_tag="WJK66_001387"
     CDS             complement(1267..2823)
                     /gene="dnaA"
                     /locus_tag="WJK66_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEZ7579094.1"
                     /translation="MTLAEFWPLCLRRLHDMLPHGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     GSEEMPVQEVLLDELPSEEPVKPAASKTAADILAERMKNLPHEPRHTAEPASRPESEV
                     LAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            3116..3250
                     /gene="rpmH"
                     /locus_tag="WJK66_001388"
     CDS             3116..3250
                     /gene="rpmH"
                     /locus_tag="WJK66_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEZ7579095.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            3253..3618
                     /locus_tag="WJK66_001389"
     CDS             3253..3618
                     /locus_tag="WJK66_001389"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEZ7579096.1"
                     /translation="MDYRFGKQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLGH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVRRKFDRATAKQAR
                     VELAQLMFGNPATGCGKQV"
     gene            3683..3904
                     /gene="yidD"
                     /locus_tag="WJK66_001390"
     CDS             3683..3904
                     /gene="yidD"
                     /locus_tag="WJK66_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEZ7579097.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHDPVP"
     gene            4076..5713
                     /gene="yidC"
                     /locus_tag="WJK66_001391"
     CDS             4076..5713
                     /gene="yidC"
                     /locus_tag="WJK66_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEZ7579098.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQQQAVTA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFILFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYSLEGDKVEVRLSAPETRGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGGQSVCAAGECSIDI
                     KRRNDKLYSASVSVPLAAIPAGAKAETAVNLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIVVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRTQGEVVS"
     gene            complement(5794..6507)
                     /locus_tag="WJK66_001392"
     CDS             complement(5794..6507)
                     /locus_tag="WJK66_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEZ7579099.1"
                     /translation="MVSPVLYLIPTPLGTPDTPCLLPHEQQAIVGLTDFVVEAEKTAR
                     AHLKHLGVTMPIRELNLQTLNEHTDLKTLLELLKPLQEGRSMGIVSEAGCPAVADPGA
                     NLVALAHKHGFEVRPLVGPSSLLLALMASGANGQSFAFKGYLPSEKNERIQSLKALEQ
                     RSRQQNETQLFIETPYRNDVLLADAVENLHSETRLCVAADLTLPTQEIISRTIAQWRK
                     RKEMPNLKKRPTIFVMYAG"
     gene            complement(6566..7156)
                     /gene="maf"
                     /locus_tag="WJK66_001393"
     CDS             complement(6566..7156)
                     /gene="maf"
                     /locus_tag="WJK66_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HEZ7579100.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLTGRFPEALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMQRHIDKTVVVMRKLDELHILRYLEREPDAVYCSCALKSEGL
                     GALLIERIESSDPNALIGLPVFRLVDFLKNEGVEVL"
     gene            7304..7807
                     /locus_tag="WJK66_001394"
     CDS             7304..7807
                     /locus_tag="WJK66_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEZ7579101.1"
                     /translation="MSDPNLIDPEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSN"
     gene            7841..8020
                     /gene="rpmF"
                     /locus_tag="WJK66_001395"
     CDS             7841..8020
                     /gene="rpmF"
                     /locus_tag="WJK66_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEZ7579102.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
BASE COUNT         2003 a         2436 c         1949 g         1768 t
ORIGIN      
        1 ttccgacagc cgaacaaaac cccgaatccg tctgcacggt tcggggcata tctccaatac
       61 gggcatcgtg ctccggaaaa ccgtcaaatt cgcatcggca tcacgatata tttgaaattc
      121 ggattgttcg gcacggtaaa cagcgtcgag cggttggcat cgccgaaggc aagctgcata
      181 tcgtcggaat ggatgttgcg caacacgtcc atcagatagc cgatattgaa accgacttcg
      241 agttcgccgc cctgataggc aatctcgatt tcttcgcgcg cttcttcctg ctcgttgttg
      301 ctgcacacga cgctcaacag gccggattgc aggaacaggc gcgcgccacg gaatttttcg
      361 ttggcaagaa tggacacacg ttccagcgcg cccaaaagtt cggcacggga caaaacgaaa
      421 atcttgtcgt tgtccaaagg aatcacgcgg ttgaaatcgg ggaatttgcc gtcgatgacc
      481 ttgctgacga tggtcgtgcc gttgcattgg aaacgcacct gtttgtccag cagctcgatt
      541 tgaatcggat cgtcggggtt gttcaacagt ttgaacagtt ccagcaccgt tttgcgcggc
      601 aaaatcactt cggcgcgcgg caaatccgca tcaatcgcgc aggctgcata ggcaaggcgg
      661 tgtccgtcgg tcgccacaag gcgcaactgg ctgccctcaa cctgcatcag cagaccgttg
      721 agataatagc ggatgtcctg caccgccatg ctgtactgca cttgcgacag catggttttg
      781 aaacgctcct gctccagcga gaaagtcgcg ctgatgtcct cgccgacatt catcatcgga
      841 aaatcggcgg caggcagggt ctgcagggca aaacgcgatt tgcccgcctt cagcgtcaga
      901 cggctgtcgt cccaatccag cgacaccagc gcaccggcag gcagtgcccg taaaatgtcc
      961 tgaaatttct tggcattggt ggtgatgcgg aagtcgtccg cgccgccctc gggacccgca
     1021 gtgtcgattt ggatttccaa atcggttgcc aagagtttgg tctgaccgcc ttttccctca
     1081 atcaggacgt tggacaggat gggcagggta tggcggcgtt cgacgatgcc ggtaacggct
     1141 tgcagcggct tgagcaggct gtcgcgttcg gcttgtaaaa tcaacatttt cgctccttaa
     1201 atcggttgga ttttttaccc ttcggacggc atgggcaata tcatgccgtc tgaaaggcgc
     1261 gttcggtcag ttttgaatca gaatcagcag tttctcgtaa tcctgcgcca actcggggtc
     1321 ttcctcgcgc agtttcgcca ctgccctgat gccgtgcata acggtcgtat ggtcgcgccc
     1381 accgaacgaa tcgccgatag acggcaggct caaagtggtc agttctttgg tcaggctcat
     1441 cgccacctgg cgcggacggg caatgtttcg tgtccgtttc ttaccgagca catcgctgat
     1501 tttgatacgg taatatttgg caacggcatc aataatgata tcggcggtaa tgaccttatg
     1561 cttctcggca ataatgtcct gcaaagcggt acgcgccaaa tcgatgtcga tgacgggacg
     1621 gttcataaag cggctgctcg ctccgacacg gttgaacgcg ccttcaagct cgcgcacgtt
     1681 ggaacggatc agattggcaa tgaacaacgc ggcttcgtct tcgatactga tgcccgccgc
     1741 ttccgccttt ttctgcaaaa tagcgatacg catttccaat tcgggcggct cgagttccaa
     1801 agtcagtccc catgaaaaac gggatttgag gcggtcgtcc ataccttcga ttttggcggg
     1861 taaaacatcg caagtgagga tcagctgttt tttctcgttg tggaaatggt tgtacagata
     1921 gaaaaactct tccatcgtac ggtcttttcc tttaataaac tgaatatcgt cgataatcag
     1981 caggtcgtat tgcttgtatt gctgcttgaa tacgtcatag gtattgttgc gaaccgcctt
     2041 cataaagctg cggatatagt cgtccgaatg catatagcgc actttggcat cgggacggtt
     2101 tttcaaaagc tcgttgccga ccgcctgtac caggtgggtt ttgcccaaac ccgtgctgcc
     2161 gtacaggaaa aacgggttgt agctctgccc cgggctttcc gcaatcgcct gcgccgcagc
     2221 cgccgcaagg cggttgccct tgccttcgac caatgtgtcg aacgtatagt ccggcgacag
     2281 attggtctgc tcgtaacgtg cctcttccgc atcgcgctgc gcatccgtcc gcgctttcgc
     2341 aagcacctcc gattccggac gggaagcagg ttcggcagta tggcgcggct catgcgggag
     2401 gtttttcata cgttccgcca gaatatccgc cgccgttttc gacgcagcgg gtttgacagg
     2461 ctcttcagac ggcagctcgt ccaacagaac ctcctgcaca ggcatctcct ccgaccccgc
     2521 gtgcaaggac ggctcggcag gttcgacagc accttcaacc gccgccatct cataacgcac
     2581 gccttctccc ggtttgaata cgaaggcgga acggccggca gccaactctt ccctcaccgc
     2641 ttctattttt ccggcaaact ggctcttgag catattgcag gcaaactggt tcttgccgta
     2701 caccacccat acgcccccct cctcaccgac ggtaaggggc gcaatccatt gcgcaaactg
     2761 cccgtgaggc aacatatcgt gaagacggcg gaggcacagc ggccaaaact ctgctaatgt
     2821 catggatagg ctcgaatcgg taaaaatgaa atcgaaaaca aagaaaatat aatattttca
     2881 aaaagaaaac aaatctgttg aacacacatc ggttcaaaat gcagcgaacc gaattatacc
     2941 gactcgccga tattttatcc acaacccgtg caaaatttat ccacagaaag gcggcagaaa
     3001 ttcgcaggca atcgggcaat cctcctgcaa agtttctata ttgattgaca aaagcggcaa
     3061 attggagtgt aattcacggt ttaattatct acccattcta tttttaggaa acatcatgaa
     3121 acgcacttat caaccttccg ttaccaaacg caaacgcacc cacggcttcc tggtgcgctc
     3181 caaaacacgc ggcggccgcg cagtattggc tgcacgccgc gccaaaggcc gcaaacgcct
     3241 ggcagtgtaa ttttggacta ccgcttcgga aagcagtacc gcttgttaaa aacggatgat
     3301 ttttcatccg tttttgcatt cagaaaccgc cgcagccgcg acctgctgca agtttcgcgt
     3361 tcaaatggca acgggctggg ccatccccgc atcggtctgg tggtcggcaa aaaaaccgcc
     3421 aaacgcgcca acgaacgaaa ttatatgaag cgcgttatcc gcgactggtt cagattgaac
     3481 aaaaaccggc tgccgccgca agatttcgtc gtgcgcgtcc gccgtaaatt cgacagggct
     3541 accgcaaaac aggcaagggt ggaactggca caactcatgt tcggcaaccc cgcaaccgga
     3601 tgcgggaaac aggtatgatt agaacggtac tctgcaggca aggttcagac ggcaacgggt
     3661 ttcccataca aggaacatcc cgatgaactt tctattgtcc aaactcctgc tgggactgat
     3721 acggttctac caatattgca tcagcccgct gattccgccg cgctgccgtt atacgccgac
     3781 ctgttcgcaa tacgcggtcg aagcggtcaa aaaatacggc gcattcaaag gcggccggct
     3841 cgccatcaag cgcattgcac gctgccaccc tttcggcgga cacggacacg accccgttcc
     3901 ctgacccgac gcaatattca aattgcacgc tttcctttta tttcccatcg gtttctatat
     3961 aatgccgtct gaaacttcgg gctggcggca cgaccgtcgg gtatgaagcc cacccttatt
     4021 ccccgtctat cggaacacgc aacctgcggc atttccgacc attcaggaaa ctcttatgga
     4081 ttttaaaaga ctcacggcgt ttttcgccat cgcactggtg attatgatcg gatgggaaaa
     4141 gatgttcccc actccgaagc ccgtccccgc gccccaacag gcagcacaac aacaggccgt
     4201 aaccgcttcc gccgaagccg cgctcgcgcc cgcaacgccg attaccgtaa cgaccgacac
     4261 ggttcaagcc gtcattgatg aaaaaagcgg cgacctgcgc cggctgaccc tgctcaaata
     4321 caaagcaacc ggcgacgaaa ataaaccgtt catcctgttt ggcgacggca aagaatacac
     4381 ctacgtcgcc caatccgaac ttttggacgc gcagggcaac aacattctaa aaggcatcgg
     4441 ctttagcgca ccgaaaaaac agtacagctt ggaaggcgac aaagttgaag tccgcctgag
     4501 cgcgcctgaa acacgcggtc tgaaaatcga caaagtttat actttcacca aaggcagcta
     4561 tctggtcaac gtccgcttcg acatcgccaa cggcagcggt caaaccgcca acctgagcgc
     4621 ggactaccgc atcgtccgcg accacagcga acccgagggt caaggctact ttacccactc
     4681 ttacgtcggc cctgttgttt atacccctga aggcaacttc caaaaagtca gcttctccga
     4741 cttggacgac gatgccaaat ccggcaaatc cgaggccgaa tacatccgca aaaccccgac
     4801 cggctggctc ggcatgattg aacaccactt catgtccacc tggatcctcc aacccaaagg
     4861 cggacaaagc gtttgcgccg ctggcgaatg cagcatcgac atcaaacgcc gcaacgacaa
     4921 gctgtacagc gcaagcgtca gcgttcccct tgccgccatc ccggccggcg cgaaagccga
     4981 aaccgccgtc aacctgtatg ccggcccgca aaccacttcc gtcatcgcaa acatcgccga
     5041 caacctgcaa ctggccaaag actacggcaa agtacactgg ttcgcctccc ccctcttttg
     5101 gcttttgaac caactgcaca acatcatcgg aaactggggc tgggcaatcg tcgttttgac
     5161 catcatcgtc aaagccgtcc tctacccgct gaccaacgcg tcctaccgct cgatggcaaa
     5221 aatgcgcgcc gccgcaccca aactgcaagc catcaaagag aaatacggcg acgaccgtat
     5281 ggcgcaacaa caagcgatga tgcagcttta cacagacgag aaaatcaacc cgctgggcgg
     5341 ctgcctgcct atgctgttgc aaatccccgt cttcatcgga ttgtattggg cattgttcgc
     5401 ctccgtagaa ttgcgccagg caccttggct gggttggatt accgacctca gccgcgccga
     5461 cccctactac atcctgccca tcattatggc ggcaacgatg ttcgcccaaa cctatctgaa
     5521 cccgccgccg accgacccga tgcaggcgaa aatgatgaaa atcatgccgt tggttttctc
     5581 cgtcatgttc ttcttcttcc ctgccggtct ggtattgtac tgggtagtca acaacctcct
     5641 gaccatcgcc cagcaatggc acatcaaccg cagcatcgaa aaacaacgca cccaaggcga
     5701 agtcgtttcc taaatgccgc agcatgaaaa atgccgtctg aaacctgttc agacggcatt
     5761 tttattgttc accccctatc ggggcggaaa tcttcaacct gcatacatca caaaaatcgt
     5821 cgggcgtttt ttcagattgg gcatttcttt ccttttccgc cactgcgcga ttgtccggct
     5881 gatgatttcc tgtgtcggca aggtcaaatc cgcagctaca cacaagcgcg tttcagaatg
     5941 caggttttcc accgcatccg ccaacaacac atcgttgcga tacggcgttt cgataaacag
     6001 ctgcgtttca ttctgctggc gcgaacgttg ctccaaagcc ttcaaactct gaatccgctc
     6061 gtttttttca gacggcagat aacccttaaa cgcaaaactc tgcccgttcg cacccgaagc
     6121 catcaaagcc agcagcaggc tggaaggccc gaccagcgga cgcacttcaa aaccgtgttt
     6181 atgcgccaaa gccaccagat tcgcacccgg atcggcaaca gccgggcaac ccgcctcgct
     6241 gacaatgccc atactgcgcc cttcttgcaa aggtttcagc aattccagca aagtcttcaa
     6301 atccgtgtgt tcgttcagcg tttgcaaatt cagttcgcgg ataggcattg tcacgcccaa
     6361 atgtttcaaa tgcgcgcgcg ccgttttttc cgcttccacg acaaaatctg tcagcccgac
     6421 aatcgcctgt tgttcatgcg gcaacaggca cggcgtgtca ggcgtaccca aaggcgtagg
     6481 aatcaaatac aaaacaggag acaccattcc ctcactcatc agttaaaaat gccgtctgag
     6541 cctttcagac ggcataaacg ggcagttaca gaacctccac gccctcattt ttcaagaaat
     6601 cgaccagacg gaaaaccggc aaaccgatta aagcattcgg atcgctgctt tcaatccgtt
     6661 caatcagcag cgcgcccaag ccctcgctct tcaacgcaca cgagcaataa accgcatcag
     6721 gctcgcgctc caaatagcgg aggatgtgca gctcgtccaa cttcctcatc acgaccaccg
     6781 tcttatcgat atgccgttgc attctgcccg taaccgtatt tagcagaacg accgcgctgt
     6841 aaaactcaat ctccctgccg ctcaaatgca tcaacatctt ttgcgcatta gcaaggttca
     6901 tcggcttgcc ccactgcctg ccgtcgcacc acgccacctg gtccgcaccg acaatcaacg
     6961 cctctgggaa acgcccggtc aacgaccgcg ccttaccctc ggcaaggcgc aatgccgtct
     7021 gaggggcgga ttcccccaac atcggcgttt cgtcaaaatc gggggacgcc gcctgaaagg
     7081 caatgccgag cctttccatc tgttcgcggc ggaaaaccga actcgtaccc aaaatcaaag
     7141 gcagttccaa acccatccca tcctccttac cgttgaaaac acgcccgaag gggcagtaaa
     7201 atccagccat gcgccgaaac acggataccc gccttcggcg taccgcaaca tttttcttaa
     7261 aaatattgac gttagaacat ctaaattata tcatatcccg tttatgtcag accctaattt
     7321 gattgacccg gaaatttttg ccgccgaagg gcagaacctg caaggcagtt ttctgctgga
     7381 agaattggat gaacgcgtca gttcgcacga ttatcccgcc gacaggcaga ccaaaatatc
     7441 gtttacactg accggcggtc gcgaccggct gcaacgcctg ttcctcgacc tgaacgtcaa
     7501 agccgatatg cccctgattt gccagagatg tatcaaaccc atgccgttca tgcttgatga
     7561 aagcagccgt atcgtcctgt tttccaacga agagtccttg gacgaatcca tgcttgccga
     7621 cgaagaactc gaaggcatac tgattgaaaa agaactcgac gtgcgcacat tggtagaaga
     7681 ccaaatcctg atgtccctgc ccttttcgcc gcgacacgaa gactgcggcg acaatgggac
     7741 actggaagaa gtcaatcggg acaaacccaa cccctttgct gttttggcag gtttgaaaag
     7801 caattgatta ggacacagtt tatttatcta ggagcttgaa atggccgttc aacaaaacaa
     7861 aaaatcccct tccaaacgcg gtatgcaccg ttcgcacgac gcgctgaccg cgcctgcact
     7921 gtctgtcgac agcacaaccg gcgaagtaca ccgcccgcac cacatctccc ccaacggtat
     7981 gtaccgcggc cgcaaagtgg tcaaagccaa aggcgaataa tccctattcg actgactgaa
     8041 aaagccagaa cattgccatg caattactgg ctttttttgc attggacgca ccatccgtcc
     8101 aaactttcgc catacgtcaa cacacagggg caaagcgttc cgtataataa cccgtg
//
LOCUS       DAWKOG010000088         8021 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.088, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000088 DAWKOG010000000
VERSION     DAWKOG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8021
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.088"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(68..814)
                     /locus_tag="WJK66_001396"
     CDS             complement(68..814)
                     /locus_tag="WJK66_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ7579103.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(993..1664)
                     /locus_tag="WJK66_001397"
     CDS             complement(993..1664)
                     /locus_tag="WJK66_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEZ7579104.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="WJK66_001398"
     CDS             complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="WJK66_001398"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEZ7579105.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDKPLMMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(2481..2963)
                     /locus_tag="WJK66_001399"
     CDS             complement(2481..2963)
                     /locus_tag="WJK66_001399"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEZ7579106.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(2995..3684)
                     /locus_tag="WJK66_001400"
     CDS             complement(2995..3684)
                     /locus_tag="WJK66_001400"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEZ7579107.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTIGIFER
                     HEAVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLEIGLASAD
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAISIADTLKRAEGGQISATVERTS
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGIRPLLVQGDARNLKLTQPQD
                     AYIVRLLLNAV"
     gene            complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="WJK66_001401"
     CDS             complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="WJK66_001401"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEZ7579108.1"
                     /translation="MTTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIQVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGAAAEEKMETKPVVHTETKRSGKLKVIRADENELAAHG
                     QYLDGLGEACIWRKEAVPSENGGTDA"
     gene            complement(4571..5881)
                     /locus_tag="WJK66_001402"
     CDS             complement(4571..5881)
                     /locus_tag="WJK66_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ7579109.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLSEKVRHKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKP"
     gene            complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="WJK66_001403"
     CDS             complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="WJK66_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEZ7579110.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            complement(6056..6355)
                     /locus_tag="WJK66_001404"
     CDS             complement(6056..6355)
                     /locus_tag="WJK66_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579111.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNM"
     gene            6706..7902
                     /locus_tag="WJK66_001405"
     CDS             6706..7902
                     /locus_tag="WJK66_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ7579112.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAAVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
BASE COUNT         1895 a         2403 c         1949 g         1774 t
ORIGIN      
        1 ataaatttgc cacaaaaaag ctgcctcaaa tgaatacccg ggcagctttt tgttgatatg
       61 actccaatca gcggtgttgc ggattgtaac gtttttccaa acgcaggaat atccagccta
      121 agaaagtcgt catcaacaga taaatcaggg cgacggtgta aagcggttct tcataaaccg
      181 aataccggcc cgtaatcgta ttctgaacat acgccaactc cgccacagca atgaccgaca
      241 gcagcgagct gtctttcaag agcgtgatga actcgctcgc caaaggcggc agcatgcggc
      301 gcaatgcctg cggcagaatc acatagcgca tcgcctgcgg ataggtcagc cccaaagaac
      361 gcgccgcctc catctgtcct ttgtctatag actggatgcc cgcgcggaaa atctcacaga
      421 tatacgcccc cgagttggcg atcagtgcca aagaaccggc aatcagcggc ccgtatccgc
      481 gacgcagcgc gattgccgcc tcgccgctga ccaaaatgcc gtctgaagga tggacgaaaa
      541 acggaaacca cacatacgcc caaatcacaa tctgcacaaa cagcggcgta ccccggaaca
      601 gcgtaacata cagcagcgaa actttacgca acgcccacgc cagcacgcgc atcggcgcac
      661 cggctttttc caagtgaatc aggcgcgcca acgccaacaa cagacccaat accgaaccgc
      721 ccgccgtcgc cacgaccgtc agccccaagg tcgtcagtgc gccgtaaaga aacatccagc
      781 ggtattcgta aataatgtca aaacgaaaat ccataaaccg tccgtatcaa aaaccggcgg
      841 aactgccgcc gttgcaaaat aatccgccat tttaccgtaa aaaccgccgc ctgaactttt
      901 ttatcgcggc agacggcggt tgcgcgtctc cgcaaaaatg cagggcgcgc ggttttcaga
      961 cggcatttgc cgttcaaagc cgtgcggtgt ctttaccaaa tgcccaacca ttcgcccacg
     1021 gcatccatcc aatccttatt gcccccgccg ctcctgcctg ctcggcggta cgcccacggc
     1081 gcttgcggat ttttagcttt ccacaatcct ttgcgttccc tttccgcctg aatttgagcg
     1141 tcggcataat cggcaaaatc cgccttatcc tgctgttctt tagcataact tttataatgc
     1201 cacgccgccc cgtcctgcac ctgcatcagg ttcaaatcgg ttttgccgac agaaacctgc
     1261 gccacttcgc gctggtagcg gtcggtatcg aacacgcgca cgctgacttt cctgccttcc
     1321 gccgccgcgc gcaggttgtc gcgcgaacgc gtgccgtaag cctgtttcat ctccggcgca
     1381 tcgatatacg ccatccggat tttgtgtttc gcgccgtcgc cgtcgataac gtgaagggtg
     1441 tcgccgtcat agactttgga caccgtgcct gtgtagcggt ggccggattt cgccgatgct
     1501 cggcggcggg cgggcgcgtc ggaacccgcg tcccctgccg cgccgagtac gtcgagtacg
     1561 gcaaccgccg tccgcaccgc ctcgctgccg taccccgtat aacccaacgc acccaaaagc
     1621 gacagggcga cgggaagcca tttcatgatt tttttaatct gcatattttt caaatgccga
     1681 tgccgtctga acatctctga accggatttc agacggcatc ttaacgtcag gattaccctt
     1741 ggcagggata gatgactttc gcaccctctt ccgtccccaa aatcaacaca tcggcggcat
     1801 cgcgggcgaa tattccgttt tcgagtacgc cggtgatttt gttgatttcg tcttccatca
     1861 tcagcggttt gtcgatattc aagccgtgga catcgacgat ttggttgccg taaaaagtgg
     1921 tgtagccgat acgcaattca ggctgtccgc ccatagcgag cagtttgcgc gaaacaaggg
     1981 aacgcgcgct ttcgacgact tccacgggca gagggaattt gcccaaacgt gaaacatatt
     2041 tgctttcgtc cgcaatgcag acgaattttt ctgatgcgct ggcgacgatt ttttcgttga
     2101 ggtgtgcgcc gccgccgcct ttaatcattt gcagggcgtg gttcacttca tccgcaccgt
     2161 caatatagac cgccaacccc gatacttcgt tcagggaaac gacgggaata tcatattggg
     2221 caagcagttc actggatttt ttggaagtag ataccgcgcc tttgattttt ttgccgctct
     2281 tacccaaggc ttcgatgaaa aagttgatgg tcgaaccggt accgatgccg atgtattcgt
     2341 tttcgggtac gaattcgact gctttttcgg cggcgatgcg cttgagttcg tcttgtgtcg
     2401 tcatattttt gtcctttggg aaaccgtatc aacaaacagc cgccatctta acattttttt
     2461 gcacgtcctg cccgccgcgt tcaaatcctt accagcaata ccgccgcctg cgcctctatg
     2521 ccttccatgc gcccgagata accgagtttt tcgttggttt tgcctttgat gttgacgcac
     2581 gaaatgtcta tgcccaaatc ggcggcgatg ttggcacgca tttgcggaat atgcggcgcg
     2641 agtttgggtt tctgtgcaat cacggtcgta tcgacattga ccgcctgcca accctgcgcc
     2701 tgaacgcttt gatacgccgc acgcaaaagg acgcggctgt ccgcatcttt gaactctgcg
     2761 gcggtgtcgg ggaaatggct gccgatatcg cccaaacctg ccgcaccgag cagcgcgtcg
     2821 gtaacagcgt gcagcagcgc atcggcatcg gagtgtccga gcagcccttt ttcaaatggg
     2881 atttcaactc cgccaagtat cagctttctg ccttcggtca gttggtggac atcgtagccc
     2941 tgtccgatac ggatgttcgt catcgtttgt gttcctgatg ttttgaattg aagttcagac
     3001 ggcattgagc agcagcctga cgatgtatgc gtcctgcggc tgcgtcagtt tcaaattgcg
     3061 cgcgtcgccc tgcaccagca aagggcggat gcccaatttt tccacggcgg acgcttcatc
     3121 ggtaatgccg tccaagtttt ccgcagccaa tgcgcggtgc agcagccccg cgcggaaaag
     3181 ctgcggcgtt tgtgcctgcc aaaggctcgt ccgctcgaca gtggcactga tttgtccgcc
     3241 ttctgcgcgt ttgagcgtat cggcaatgga aattgccaaa atcccgcctt cggcggcgtt
     3301 gcccgcctgt tctatcaacc gcgtcaaagc ttcagacggc aggcagcagc gcgcggcatc
     3361 gtgtaccaga atattgtcat ccgccgatgc caagccgatt tccaacagtt ttgccacacc
     3421 gtttcggacg gtttcggcgc gggtctgtcc gccgtttttc cacacccgaa cctgtggaaa
     3481 tgccgtctga accttatcgg caaacgtgtc ttcgggcgag acgacaacga cggtcaaatc
     3541 gacggcctca tgccgttcaa aaatcccaat cgtatgttct aaaacggttt tgcttccgat
     3601 ttcgacatat tgcttgggtt tgtccgcacc gaaacgcgcc ccgatgccgg cggcgggaat
     3661 cagcgcgata tttttgcgtt tcatgcgtcc gtcccgccgt tttcagacgg cacggcttcc
     3721 ttgcgccaga tacaggcttc gcccaagccg tccaaatatt gcccgtgcgc cgccaactcg
     3781 ttttcgtccg ccctgatgac tttcagtttg ccgctgcgtt tggtttcggt atgcaccaca
     3841 ggtttggttt ccattttttc ctctgcggcc gcacccatca ggtcgaactg ccgccgcgtc
     3901 atagcaagat agacttcgcc caaaagttcg cagtcgatca atgcgccgtg caggacgcgc
     3961 ttgctgcggt cgacggaaaa acggttgcac aaggcatcca ggctggcttt ctgcccgggg
     4021 aacatttcgc gcgccatcgc cagggtatcg gtaacggtac agccgagttc ctcaacggtc
     4081 ggcaacccca tccggcggaa ctccatattg aggaagccca cgtcgaattt ggcattgtgg
     4141 ataatcagtt ccgcaccgcg caggaaatcg gcaatctgcc tgccgacctc tgcaaacggc
     4201 ggcgcgtttt tcccttccaa aacctgtatc gtcaagccgt ggacgcgtgc cgcctcttcg
     4261 ggcatatcgc gctcggggtg gacatagagg tgcaggtttt tgtcggtcat ttggcggttg
     4321 accatttcca aaccggcaaa ctcgaccaag cggtcgccgc cgtcggcata cagaccggtg
     4381 gtttcggtat cgaggatgat ttggcgtgtc gtcatatcgg tgtctttctt ctatcttcgt
     4441 aaattgctta ttttttaagc aatgtatttt tctgttttca tttcaatgca caaacccact
     4501 tattcacagt gtgttcacaa cattgggcag gcggattgtg tattttgggg acaatttttt
     4561 cagacggcat tcaaggtttt ttcctgattg ccgccgcgcc taaaaaccgc ctttcgcgct
     4621 taatcaaaaa taccgacaac ggaatattgc ccaaagcgac aatcagatac aacaaggaaa
     4681 tgctgtcaaa caaaaacagc aacaccgcgc tcaaaacggc ggcggaaacc ataaaaatac
     4741 cgttaacgat attgttggcg gcaacggcgc gggcgcggaa agtctcgcta ctggcggttt
     4801 gcagccaagt atagagcgga acggagaaaa atccgccgaa aaagccgatc agcgtcatca
     4861 ccgccatcac gggatatgcc catccttgcg ataaaaacca aaaaatgccg ttcagccctt
     4921 caaaacggtg tccgtgcgtc agccacacca aaaccaggcc gcaaaccgtc aaacccaacg
     4981 caccaaccgt tacccaagcc aacatcagcc gttccctgct gaacttggca cacagtaccg
     5041 aaccggcggc aataccgatg gaaaacaggg caagcatcag gttgaaaaca ttgtcgttgc
     5101 cgcccagatg gatttgggta aaggtcggca gttgcgtggt ataaaccgcg ccgacaaacc
     5161 aaaaccacga aataccgata atggcggtaa aaacgggctt gtgccgcacc ttttcactca
     5221 gcagggattt tgtgccacgg acaatattcc actcaatttg tgtatcggca gccttggcgg
     5281 gtacagacgg cataaacagg ctgccgaccg tgcctccgac ggcgaccagc aaaaccagta
     5341 tcccgacaat ataaggcggt acacctgcca ccgccgttcc caaaatctga ccgaacagga
     5401 tggcgacaaa cgtacccgat tcaatcaggc tgttgcccat catcaactct ttgtcgtcga
     5461 gataatcggg caggatggcg tatttcagcg gcccgaacag cgtcgattgc gcgcccatgc
     5521 aaaacagaca cgccaaaagc agcggggcag accggatata aaacccgtat gccgccaccg
     5581 ccataatgat catttccagc accttgaccc aacgcgccaa aacggccttg tcgaatttgt
     5641 tacccaactg ccccgacagc gaggaaaaca ggaaatacgg caaaataaac agcaacgcgc
     5701 ccaagttcaa catctgtccg gcaggcagga agccgttttg ccccaaaccg taaaacccaa
     5761 tcatcacaaa cagcgcggtt ttgaacacat tgtcgttgaa cgcgccgaga aactgcgtag
     5821 cgaaaagagg tgcgaaacgg cggcttttaa ccagtcccaa accgcctttt ttagcgtaca
     5881 tcgttttccc tctctttttc aatcagttta cttgtcgaat catcatccat caggatgcgg
     5941 tgcgccggcc cttccaagtc gtcaaactgc ccgtttttgc ccgaccacca aaaaaaccag
     6001 ccgatgacaa acgccaaaat aatgctgatg ggcaccaata taaacatgct ttccatcaca
     6061 tattccctgt caaatcgttc aaaacaaaag tctgccccga cacggtcaga tattcgttac
     6121 gcaaagttcc gacaggtgct tcgtcaaaaa acagctcgat acggtctttg accacgcgcc
     6181 aatattgggg gatttccgtc tgaccgaacg gcgacaggac atgattttcc attccgcctt
     6241 caagtttgac ggcaaaacgc ccgctttgcg gccgcgcctc tgactcgtcg tcggcaagca
     6301 ggatgaaaaa gcctatatgc cgtcccgatt ggtcatgaat actgaaataa tgcataaatt
     6361 tcccacccgc cttttttcag acgacaccaa ctaaaaacag ggcgaatgta ccagtttgga
     6421 tgggaagaat gcaaagaaat tctccctccc ccagccgaaa acaccggcaa accgcatatc
     6481 cccctttttt ccgtcaaaat acccgacttc cgccattttc acgcaaacgc ccgattaagc
     6541 caagcaattg caaagatttt ttgttagaat agcctgcttc ttttatcaac cttttcagac
     6601 ggccccacta ctttcccgcc caggaaggca aaacggattc ggcacgaatc cggttagtat
     6661 ccgtgtccga ttccaatgcc gtctgaaact ttccggagta agaaaatgtc ccaaaaattg
     6721 atcttggttt tgaactgcgg cagctcgtcc ctcaaaggcg cggtcctgga taacggcagc
     6781 ggcgaagtcc tgctcagctg ccttgccgaa aaactcaacc tgcccgatgc ctacatcaca
     6841 ttcaaagtaa acggcgaaaa acacaaagtc gatctgtccg cacatcccga ccacaccggc
     6901 gcggtcgaag ccctgatgga agaactcaaa gcccacggcc tcgacagccg catcggcgcc
     6961 atcggccacc gcgtcgtcag cggcggcgaa ctgtacagcg aatccatcct cgttgacgac
     7021 gaagtcattg ccggcatcga aaaatgcatc ccgctcgccc ccctgcacaa ccccgcccac
     7081 ctcttgggcc tgcgtgccgc gcaaagcatt ttcaaaggcc tgcccaacgt cgtcgtattc
     7141 gatacctcct tccaccaaac catgcccgaa gtcgcctaca aatacgccgt tccgcaggag
     7201 ttgtatgaaa aatacggcct gcgccgttac ggcgcgcacg gtaccagcta ccgcttcgtc
     7261 gccgacgaaa ccgcgcgctt cctcggcaaa gacaaaaaag acctgcgtat ggtcattgcc
     7321 cacttgggca acggcgcgtc catcaccgcc gtcgccaacg gcgaatcgcg cgacaccagt
     7381 atgggcctga ccccgctgga agggctggta atgggtacgc gcagcggcga catcgatcct
     7441 tccgtattcg gcttcctcgc cgaaaacgcc aatatgacca tcgcccaaat cactgaaatg
     7501 ctgaataaaa aatccggtct gctcggcatt tccggcctgt ccaacgactg ccgcaccatt
     7561 gaagaagaag ccgccaaggg gcataaaggc gcgaaattgg ccttggatat gtttatctac
     7621 cgccttgcca aatacatcgg cagtatggcg gttgccgcag gcggtttgga cgcactggtc
     7681 tttaccggcg gcatcggcga aaactccgac atcatccgcg aacgcgtgat cggctacttg
     7741 ggcttcctcg gtctgaacat cgaccaagaa gccaacctga aagcccgctt cggcaacgcc
     7801 gccgtgatta ccactgccga cagcaaagcc gttgccgtgg tcattccgac taacgaagag
     7861 ctgatgattg cccacgacac tgcccgtttg agcggtctgt aagattcaat ccccacgcga
     7921 actgcctccg aaaacggagg cagttttttt atccggcttt ccatgcttaa acagcaccgc
     7981 ctcttttcag acgttgacgg ttgcagccgc ttacctgaac c
//
LOCUS       DAWKOG010000089         8010 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.089, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000089 DAWKOG010000000
VERSION     DAWKOG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8010
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.089"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            281..1408
                     /locus_tag="WJK66_001406"
     CDS             281..1408
                     /locus_tag="WJK66_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ7579113.1"
                     /translation="MSNWKPNIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLIIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVAKIDA
                     IRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     DIGVLQEIAIGRDKLFIHPRLMELLRGEGNSFTSFQSLVKA"
     gene            1438..2315
                     /locus_tag="WJK66_001407"
                     /pseudo
     CDS             1438..2315
                     /locus_tag="WJK66_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245633.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
     gene            2319..2507
                     /locus_tag="WJK66_001408"
     CDS             2319..2507
                     /locus_tag="WJK66_001408"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEZ7579114.1"
                     /translation="MDGWYDFRFQNVVYAPDRSPEISRMLSSILAGYAWDTENPFVAK
                     SEQRLTALSEWVGQLESE"
     gene            complement(2584..4095)
                     /gene="lysS"
                     /locus_tag="WJK66_001409"
     CDS             complement(2584..4095)
                     /gene="lysS"
                     /locus_tag="WJK66_001409"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEZ7579115.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQGIPVKVAGRMMLKRQMGKASFATIQDVSGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNQGELTVRVSGIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRAVNGTANITYNGKEVDLESPFERLTILDAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIIDY
                     PVEVSPLARASDSKPGLTERFELFVVGRELANGYSELNDPEDQAERFKAQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
     gene            complement(4092..4244)
                     /locus_tag="WJK66_001410"
     CDS             complement(4092..4244)
                     /locus_tag="WJK66_001410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579116.1"
                     /translation="MPSETANRFQTALPRLWKKMPTSRKIRANFVIRSGRLKQIAPDL
                     FFRKTL"
     gene            complement(4250..5503)
                     /locus_tag="WJK66_001411"
     CDS             complement(4250..5503)
                     /locus_tag="WJK66_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ7579117.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCILSATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQSDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            5961..7757
                     /locus_tag="WJK66_001412"
     CDS             5961..7757
                     /locus_tag="WJK66_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEZ7579118.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVLTTDEAGVWVDSRYWEQAAKQLAGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLTAKNIRIEHPDDLLDQVWTSRPAIPAETVFVH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSYLL
                     IGKDNAVLFTDRCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESVRLIEGINPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFISLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVAAPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
BASE COUNT         1997 a         2242 c         2030 g         1741 t
ORIGIN      
        1 tagtgtatat taagtacgtc tgatatacac gataccctac gagggtgtaa gctttagttc
       61 acatttaaaa tgacctcttt aaacctgcct ttcggcaggt ttctttttag gttgtttgga
      121 aatcgtgtgc agacaaggtg taaaataggt aacaacataa aataatgcgg ttttaccgcc
      181 catatattta caaaagccaa atttttaaac atatatcctt gatatataca cggcgtaaac
      241 atatactgga aacatcttta aattttccga aattttaaat atgagcaact ggaaacccaa
      301 tattccctat aacgatttac cacccctgcc gccaaaacag gatattgaaa gcaaaaccat
      361 cctgaaacgt tgtatagccg cccgtgcatc ccttgcccgt ttaaagcagg cggcagaatt
      421 gataccgaat caagccatgc tgattaacac ccttcctgtt atggaagccc gtgcaagttc
      481 ggaaattgaa aacatcgtaa ccaccacgga caagctgttt caatccctgc aaatggatac
      541 ggaacggcaa gaccctgcca cgaaagaagc cctgcaatac cgcaccgccc tgtttgcagg
      601 ctatgaatca ctgacgagcc gccctttatg cacacaaacc gccatcatgg tctgcaacgc
      661 catcaagcac ccctacgaaa tggccatccg caaaacaggc ggcacagccc taaaaggagg
      721 caacagcgga aatgttgtct ataccccgcc cgaaggagaa gaaaccatac gcggcaagct
      781 ggcaaattgg gagcggttta ttcacgaaag cggcgattta gacccgctta tcatcatggc
      841 ggcggcacat taccaatttg aagccatcca tccgtttacg gacggcaacg ggcggacggg
      901 gcgcatattg aacagcctgc tattgattga aaaagggctt ttggatttgc ctattttgta
      961 tttgagccgc tatattatcg aaaacagggc ggactattac cgcctgcttt taggcgtaac
     1021 cgaacggcag gactgggaaa gctggataat ctacatctta gacggcgtag ccgacaccgc
     1081 cgattggacg gtagcgaaaa tagatgcgat acgccgcctg tttgagcaga cacgtcaaca
     1141 catacggaca cacgcacaag gaatctacac gcacgaactg gtaaatcttc tgtttgagca
     1201 gccatataca cgcattgcca acctagaagc ggcagggata gccaaacggc agacggcctc
     1261 taagtacctg aaagagcttt cagacatagg tgtgctgcaa gaaatcgcca tcggcaggga
     1321 caaactattc attcatccgc gcctaatgga actattgcgg ggagagggca acagctttac
     1381 ctcattccaa tccctcgtta aagcatagcc aaaataatca ataatccgga ggtcaatatg
     1441 gcaagaaggt caaaaacatt tgaagaagct gctgctgagg ttgaggaacg tttcggtcat
     1501 cgtggcatta agttggtcga gtttgagggt acagccaagc cgtgtgtaat caactgccct
     1561 aaacatggaa accaaacctg ttcgaggtac tccaatatgt tcataggaag tagctggggt
     1621 tgcccctctt gtggtaatga gcaagctgca aaagccggta tagcgaccct taggaagaat
     1681 cacatagcgt tagaaatgct gaaacaggct gtaacaggta tgaccaagca agagcgcatc
     1741 acgacgcaag cctacaatga gatgaccaaa tccgtggcag gttcaaacag catagtcctt
     1801 aacgatgtcc aaggcgatac gaccatcaac aaccatcata cgcatacgca caaccacagc
     1861 gatgccgacg gcaaagcact gtcgatgagg ctcacaccac gccccctgtt gtcagaccgt
     1921 caggcggcgg ctttcgcccg tacaggcaaa ctcacaggca gtttcgactt gtttgcttcg
     1981 gtggtcgccc cctcgcagta cacgtttgcc gttgccatgc ccgacacgtc catgtcgccg
     2041 gttatcgaaa aaggggattt ggtggtggtc gagccgcgta tgcgccctgc ggacgaggac
     2101 atcgtgctga ttgaactgtc cgacaagcgg ctggtcgtcg cgcaccttgt tatcgatatt
     2161 gcgggcagga tgctgattta tcagacgggc aggccgtctg aagcctttga cctgcccgaa
     2221 ggcagcacga ttttaggtgt ggtgctggag tcaaaaaacg gtttatgtcc gccgcacagg
     2281 caagaaggcg tgttgattcg gattaccacc cctgatgtgt ggacggttgg tacgatttcc
     2341 gcttccaaaa cgtcgtgtac gcgcccgacc gcagcccgga aatcagccgt atgctttctt
     2401 cgattttggc aggctacgcg tgggataccg aaaacccgtt cgtggcgaaa tccgaacaac
     2461 gcctgactgc cttgtccgaa tgggtcggtc agttagaaag cgaataaatc cgtaccgcca
     2521 tacaaaatgc cgtctgaatc caatcgggtt cagacggcat tgccatttca actgttttta
     2581 tgactactcg gggcgcatct gcggaaacag aatcacgtcg cggatggttt gcgaatcggt
     2641 cagcaacatg accaagcggt cgatgccgat gccgcagccg ccggtcggcg gcaggccgaa
     2701 ttccatcgcg cggatgtagt cggcatcgta gtgcatcgct tcgtcgtcgc ccgcgtcttt
     2761 ttgcgccact tgtgccttga agcgttcggc ttggtcttcc ggatcgttca actcggagta
     2821 gccgtttgcc agttcgcggc cgacaacgaa aagctcgaag cgttcggtca agcccggttt
     2881 gctgtcggat gcgcgtgcca acggggacac ttcgacaggg tagtcgataa tgaaagtcgg
     2941 gttccacaat ttgccctcgg cgcaaccttc aaacagggca agttgcaggc tgccgatgcc
     3001 cggagacgga ggcaggcttt cgccgtgttt gacgatttct tttttcagcc actccgcatc
     3061 gttcaactgc tcgtcggtgt agtgcggatt gtattttttg atggcatcga gaatggtcag
     3121 acgctcgaac gggctttcca aatcgacttc tttgccgttg taagtgatgt ttgccgtgcc
     3181 gtttaccgcg cgggaagcgt tgcggatgat gtcttccgcc atttgcatca tgcgttcgta
     3241 gtcggagaag gcttcgtaga attcgatcat ggtgaattca gggttatggc gcacagacat
     3301 accttcgttg cggaagctgc ggttgatttc aaacacgcgt tccaaaccgc cgacaaccaa
     3361 gcgtttcaga tacagctcgg gggcgatgcg caggtagagc ggaatgtcca aggcattgtg
     3421 gtgggtaacg aagggttttg ccgtcgcgcc gccgggaatc gggtgcatca tcggggtttc
     3481 gacttcgaga taatgctcgc ccaccataaa attacgcacg gattggatga tttggctgcg
     3541 tttgataaag gtattgcgcg attcttcgtt ggcaatcaaa tcgacatagc gttggcggta
     3601 tttggtttcc tgatcgctca agcctttatg tttgtccggc agagggcgca gggatttgga
     3661 cagcaggcgg atgccggaca cgcgtacggt cagttcgccc tggttggttt tgaacaaagt
     3721 gccttccgcg ccgacgatgt cgcccaaatc ccaatggttg aagtcgtcca aaacttcttg
     3781 gctcacgcct ttgttgttca gataaagctg gatttgcccg gacacgtctt gaatagtggc
     3841 aaagctcgcc ttgcccattt gacgcttcag catcatgcgg ccggccactt tgacgggaat
     3901 gccttgcgga tcgagttctt ctttgccgat ttcgccgtat tgggcgtgca aatcggcggc
     3961 gaagctgtcg cgtttgaagt cgttgggata ggcgttgcgc tgttggcgga tgttgtgcag
     4021 tttttcgcgg cgcagggcga tgatttggtt ttcgtccaac tgcggctcgg tttgcggatg
     4081 gttttgttcg ctcatagggt tttccgaaaa aataaatcag gcgcaatctg tttcagacga
     4141 cctgaccgaa tcacaaaatt tgcgcgtatt ttacgcgatg tcggcatttt tttccataaa
     4201 cgcggcaatg ccgtctgaaa gcggtttgcg gtttcagacg gcatcgttat catttgaaca
     4261 ttcccgccaa attcaataag aacaaaacgg taaaaccggt cagataaatc aagcctgcca
     4321 atgcaagggc attcatacct gatgtgagtt tgtgtttttc atcacctttg accaaacggt
     4381 aattcagcca ggcaaacaca ggagcggaca caaaagcggc aatcatcgca aatttgagca
     4441 gattcgccat tacgccgtca aaccaaaaaa tcaccgccaa accgctgccc gccacccaaa
     4501 tattccaggc aaagaattcg gcgttgcccg ttttgtcttt tccgcgcagc aggcgcacgg
     4561 gttcggcaat ggcacgggca tagccgtcca cgacggtaat cgtcgtgccg tacatacagg
     4621 caaacgcgat aaacgccacc agcgggcgcg accagccgcc gatggtaacg gcgtacatat
     4681 tgatcaattg cccgatatat ttgccgcccg ccatctgtac tgcttcgccg ttgccgtatt
     4741 gcacaaacgc acccagtgca aggaaaacca aagccaaaac cgcactggcg atataaccga
     4801 cgttgaaatc aaaaatcccg tcgcggtatt cggaaggatt gatgcgttgt ttttcggtta
     4861 cccacaaaga attgatggcg gaaatttcaa tcggcgcggg catccagccc atcagcgcga
     4921 tcaggaagcc caaaccggca agcgtccacg gtgtcggctc gataaaatcg gactgcatct
     4981 gcataccgcg cgacatagcg atgccggcgg cggcaagcgt ggcgatactc aaagtaacga
     5041 tgatgatttt ggaaacgcga tccaaagcgc ggtaacgtcc gctcaccaaa ataatcaggc
     5101 aggatgccat aatcaaggcg gcaaccgtgc cggcatcaaa catcagcgag ggaatcgcca
     5161 ttttgacgat ggcggcggtt acaatggcga ccgcgcccgc gttaatcgtg gcggagagga
     5221 tgcacaaaat caggaatacc cacaaataaa cgcggctttt ctcggcataa ccttcaatca
     5281 ggctcttgcc cgtgtccagc gtgtaatgcg cgctgaagcg gaaaaacggg tatttgaaga
     5341 ggttggtcag gatgatgatg agcgcgatct gccagccgta aagcgcgccc gcctgcgtcg
     5401 aggcaatcag gtgcgaaccg ccgaccgccg ccgaagccat cataatcccc ggtcccaatg
     5461 cgttgatttt acttttccaa gtcgaaatat gttgttcgga cataaagtct tccgtatttt
     5521 taaccgtgtt tcaacacaca gagccgcata ttcggacaca gccctatcta ttgctccaat
     5581 ttgggcggga ttgcccccaa acaaacccaa atcctaccgt cttcaaaaac aggataccgc
     5641 ccggtaggga aattttgatg aaagcgcgta ttgtaacgta atccaaatac ctgccaacac
     5701 acactattag aacttcatgc tcaaacttga ctatattttc cgtattactt ccaaaaaaat
     5761 gcataaaacg atatcttatg cctaaaattt tacaacaaac aaccttacat cgcttttttc
     5821 gcgcaaacac gcaccatccg atcagcccgt ccgttttgca gcaggctggc gatttgataa
     5881 gatggttatg tttttcagac ggcatttcag atttccgtcc atgccgtctg aagccgcaaa
     5941 acccgattgg aggaactgtt atgaataccg tatcgaatta cctgtccgca ttgcgcgaag
     6001 ccatgaaggc gcaaggcttg gatgcactcg tcatcccttc cgccgacccc cacctgtccg
     6061 aatacctgcc tgagcattgg caggcgcgcc gcgaattatc gggctttacc ggctcggtcg
     6121 gcacgtttgt cctgaccacc gatgaagcgg gcgtatgggt ggacagccgc tattgggaac
     6181 aagccgccaa gcagcttgcg ggcagcggca tcgaactgca aaaaagcggg caagtgccgc
     6241 cgtataacga atggctcgcg gcaaacctgc ccgaaaacgc cgccgtcggc atcccttccg
     6301 atatggtctc gctcaccggc aaacgcactt tggcgcaatc cctgaccgcc aaaaacatcc
     6361 gcatcgaaca cccggacgat ctgcttgacc aagtgtggac aagccgtccc gctatccccg
     6421 ccgaaaccgt gttcgtccac gaccccgcct acgtctccga aaccgccgcc gaaaaactcg
     6481 cccgcgtgcg cgccgtgatg gcggaaaaag gcgcggatta ccacttggtt tcctcgctcg
     6541 acgacatcgc ctggctgacc aacctgcgcg gcagcgacgt gcctttcaat cccgttttcg
     6601 tgtcctatct attgattggc aaagacaacg ccgtcctgtt taccgaccga tgccgtctga
     6661 atgccgaagc cgccgccgcg ctgcaaaccg caggcatcac ggtcgagcct tacgcccaag
     6721 ttgccgacaa actcgcgcaa atcggcggcg cgttgcttat cgaaccgaac aaaaccgccg
     6781 tcagcacgct tgtgcgcctg cccgaaagcg tgcgccttat cgagggaatc aacccgtcca
     6841 cgctgttcaa atcctgcaaa tccgaagccg acatcgcccg catccgcgaa gcgatggaac
     6901 acgacggcgc ggcgttgtgc ggtttcttcg ccgagtttga agacatcatc ggcaacggcg
     6961 gcagcctgac cgaaatcgac gtggacacca tgctttatcg ccaccgcagc gtgcgcccag
     7021 gcttcatttc attgagtttc gacaccatcg caggcttcaa cgccaacggc gcactgccgc
     7081 attacagcgc aacgcccgaa agccacagca ccatcagcgg caacgggctt ttgctcatcg
     7141 actccggcgc gcaatacaaa ggcggcacga ccgacatcac ccgcgtcgtc cctgtcggca
     7201 cacccaccgc cgaacaaaaa cgcgacaaca ccctcgttct caaagcccat atcgcgcttg
     7261 ccgaagccgt gttccccgaa aacatcccct cgccgctgat tgatgcgatt tgccgcaaac
     7321 ccctgtggca ggcgcaatgc gactacggcc acggcaccgg acacggcgta ggctatttcc
     7381 tcaacgtcca cgaaggcccg cagcgcatcg ccttcgccgc ccccgccacg cccgaaaccg
     7441 ccatgaaaaa aggcatggtt acctccatcg aacccggact ctaccgcccg ggaaaatggg
     7501 gcatccgcat tgaaaacctt gccgccaacc aagccgtcgc cgcccctcaa gaaaccgaat
     7561 tcggcagctt cctctgtttt gaaaccctga ccctctgccc catcgacacc cgcctgatgg
     7621 acaccgccct catgaccgac ggcgaaatcg actgggtcaa ccgctaccac gccgaagtcc
     7681 gccgccgcct cgagccgctg accgaaggcg cggcaaaagc gtggctgatc aaacgcaccg
     7741 aaccgctggc gcgttaaaca gcacggcgca aaaaatgccg tctgaaagcc cttcagacgg
     7801 cattggtttc ccaaaacatc ccgcaccgtt ttcatcttgc cgcaagcaaa tatagtggat
     7861 taacaaaaat caggacaagg cgacgaagcc gcagacagta cgaatagtac ggaaccgatt
     7921 cactcggtgc ttcagcacct gagagaatcg ttctctttga gctaaggcgc gacaacgccg
     7981 tactggtttt tgttaatccg ctatattccg
//
LOCUS       DAWKOG010000090         7968 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.090, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000090 DAWKOG010000000
VERSION     DAWKOG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7968
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.090"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..140)
                     /locus_tag="WJK66_001413"
     CDS             complement(<1..140)
                     /locus_tag="WJK66_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ7579119.1"
                     /translation="MSVQTVTYNKFFMNTNQPAVYDPLTRALHWLTVAGFIGILTTIV
                     LWT"
     gene            382..1536
                     /gene="obgE"
                     /locus_tag="WJK66_001414"
     CDS             382..1536
                     /gene="obgE"
                     /locus_tag="WJK66_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEZ7579120.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGEARSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEAQTRTVTFLEAVGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLTEKKRLEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            1619..2668
                     /locus_tag="WJK66_001415"
     CDS             1619..2668
                     /locus_tag="WJK66_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ7579121.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDACMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            3356..4777
                     /locus_tag="WJK66_001416"
     CDS             3356..4777
                     /locus_tag="WJK66_001416"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEZ7579122.1"
                     /translation="MTTIYNTLTRQKEPFSPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGKLTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGATGHTCGHHHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPPADPMPSEAGDDYTRRFYVAMNDDFDTVKAVAVLFELAGEVNKTNDAQL
                     AGRLKALGGIIGLLQRDPTEFLQGSAASDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            4948..5727
                     /gene="xth"
                     /locus_tag="WJK66_001417"
     CDS             4948..5727
                     /gene="xth"
                     /locus_tag="WJK66_001417"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ7579123.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFGR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKALSAHVYKDEKFSDHAPLVVEYDYAAE"
     gene            5714..6058
                     /locus_tag="WJK66_001418"
     CDS             5714..6058
                     /locus_tag="WJK66_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579124.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPMLVWAIFMLSRM
                     QGWLAPTKANPIWALVWLLICLPCLLIASKCLGWKGWRRVVNIFACLTVCAILSVPAS
                     LLIAFTLRDLLK"
     gene            6069..6599
                     /locus_tag="WJK66_001419"
     CDS             6069..6599
                     /locus_tag="WJK66_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ7579125.1"
                     /translation="MQGFELEDLTLWLIRDAGEDQMWIDRWAISYPVVQMSEASAGQS
                     IGEWQAGLQTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHTFQRVRCPCRAALVVPEHGGVPHGWAQKQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(6827..7942)
                     /gene="asd"
                     /locus_tag="WJK66_001420"
     CDS             complement(6827..7942)
                     /gene="asd"
                     /locus_tag="WJK66_001420"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEZ7579126.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANNVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWVDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAVHAQVADELADPAGSILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSDNPTVIDGLCVRVGAMRCHS
                     QAITLKLKKDLPVSEIETILAGANDWVKVIPNEKEASIHELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
BASE COUNT         2076 a         1987 c         2077 g         1828 t
ORIGIN      
        1 gtccacagga caatggtggt cagaatgccg atgaagccgg caacggtcag ccagtgcagc
       61 gcgcgtgtca acgggtcgta aacggcaggt tggtttgtgt tcataaaaaa cttattatat
      121 gtaacggttt gaacggacat tccgtcgggt cggaatgtca aaaaggcgcg attgtaccaa
      181 agaagttgga cataattttg tttgcaggct gaagatttgc ttaaaaattc attaagatgg
      241 gcggaacaaa tagtttgggt acggcgtgtt gaaataccgc ttatccctta taatagcgtc
      301 cgaaattctg ttcagacggc atcaaaacac acggtaatcc gtctgaaccc cccatttgaa
      361 accaacaaac aagagcattg aatgaaattc atcgacgaag caaaaatcga agtcgccgca
      421 ggcaaaggcg gtaatggcgc aaccagtttc cgccgcgaaa aattcgtacc gcgcggcggc
      481 ccggacggcg gcgacggcgg caaaggcggt agcgtctggg cggaagccga cgaaaacacc
      541 aacaccctcg tcgaataccg cttcgttaaa cgctaccaag ccaaaaacgg cgaaaaaggc
      601 cacggttccg accgttacgg cgcaggtgcg gacgacatcg tcctcaaaat gcccgtcggc
      661 accctcatcc gcgacctcga caccgacgaa atcgttgccg acctcactta ccacggacag
      721 cgcgtctgcc tagccaaagg cggcaaaggc ggcttgggca acatccactt caaatcctcc
      781 gtcaaccgcg ccccaaaaca atccacgccc ggcgaagaag gcgaagcccg ttccctgcaa
      841 ctcgaactca aagtcctcgc cgatgtcggc ttattgggta tgcccaacgc cggcaaatcc
      901 accctgatta ccgccgtatc cgccgcacgc cccaaaatcg ccaactaccc cttcaccacc
      961 ctgcatccaa acttaggcgt ggtacgcatc gacgaaaacc acagcttcgt gatggccgac
     1021 atccccggcc tgattgaagg cgcggcagaa ggcgcgggtt tggggcatcg tttcctcaaa
     1081 cacttatcgc gcaccggatt attgctgcac gtcgtcgatt tggccccgtt tgacgaaacc
     1141 gtcaaccccg ccgaagaagc cctcgccatc atcaacgaat tgcgcaaata cgacgaagaa
     1201 ctctatggca aaccgcgctg gctggtgctg aacaaactcg acatgctcga tgaagaagaa
     1261 gcccaaacgc gcaccgtcac cttcctcgaa gccgtcggct gggattaccc gaaaccggac
     1321 gaccgcttcc aattcgatat ggaaaccccg cgcctcttcc aaatcagcgc gctaacccac
     1381 caaggcacgc aggaattggt acaccaaatc aaccaatacc tgaccgagaa aaaacgcttg
     1441 gaagccgaaa aagcagaagc ggagaaagcg gcggcaaatg ttgaaattat cgaacagcag
     1501 cctaaaacgg atacgggcgt gtttaagccg gagtgaaaaa agaaagggtt tcagacgata
     1561 tttgttctac aaaaggttgt ttgaaacaat tttaaagcaa gattgaaatg gattaaaaat
     1621 gtcatctaac caatatatcc agtcgcttga gttgacaaac tttaccgttc ttccaaacga
     1681 tacttttgaa ttttctgaaa atctcaatgt tattgttgcc gaaaacggtt gtggcaaaac
     1741 acatttgtta aaaattttat acagcctgct agaagtaaca tctaacacca aaaacagatt
     1801 actgaaaaca gaattgcaaa aaagttttgc cgataaatta ctgaatgttt tccgtcccga
     1861 ttccttgggc aggttgagca aacgtttgca ggggcgcggg cgtacggaaa tcgttttaaa
     1921 actgcaaaac ggcactacgc atagtagtct gaatttttcc agtaactcat catcgcaagt
     1981 caatgttcaa agcatcggat tgaaagaaaa tgaatatact ccgacaccta tcttcttacc
     2041 cagccgcgaa ttaattacac tttgcccttg gttcacttct ttgtatcaaa accaaagcat
     2101 ccctttcgaa gaaacttggt ttgatgcctg tatgcaatta aatcacccgc ttgccaaagg
     2161 ccccagagaa acaaaaattc gagaattgct cgaacctatt gagaacgcga tgggcggaaa
     2221 agtatctgaa gaacaaggaa ggttttatct atcgctgaca aatacgggcg gtaagattga
     2281 agcacctttg gtagcagagg gtttgcggaa atttgtcatg attgcccggt taatcgcaac
     2341 aggggcattg ctggataaag gctatctatt ttgggatgaa ccggaagcta atctgaatcc
     2401 taaattgatc aaagtggctg cccgtattat ttggtcttta tctcaacagg gtattcaggt
     2461 ttttattgca acacactctt tattcttatt gagagaattg gaacttttga agcttgaaaa
     2521 taacacggat ttacctgtac gttttttctc gttgattgct tccgatgatg gaacaaaagt
     2581 ggagcaaggg aacagtatta atgatttgaa cacattgata ttgttagatg aaaacattat
     2641 gcaggcagac aggtatctga atatttgagg ttaagctatg actacaatca cagaaaagag
     2701 attaaccttt gcttttcctg aagattatta cgttaccaag tatgacgaat gggaacatta
     2761 taaaattttt cagaacagtt gtaatttacg taataaaatt gatacaaatg aaaagggaaa
     2821 gaacgggata aatcaaagtg tagatgacga taatggcagc tcaggtgtag atataatagc
     2881 ccttcatgag tcgacacttt ggctaatcga aattaaagat tattaccgac ttgggcttga
     2941 gccaaatgcc caatcaatag atgaaaaact ttctgaccta ccatatttaa tcgcaagaaa
     3001 aattcgagat tcattggcag gattggtttc tgctaaattt aaagcagaaa aacaggaaga
     3061 aaaagatttt tctcgcttgg ctttagattg taacgaaata aaaatagtcc ttcatattga
     3121 aatgcctagt ataagaagta aactttatcc ctcgtcatca gatttagcaa acttattaaa
     3181 agataagttt aaattatcag aatttacaaa aaattttgca aactgttatg cagaaccaat
     3241 tttcacaaat atcagccata ttaataatcc gcaactacgt aatgttcctt ggtccgtatc
     3301 tacaggaaca gaacagaaat tatcatccga gcaacaaaga ttaatccata atcccatgac
     3361 caccatctac aacaccctaa cccgtcaaaa agaacccttt tcccccatcg accctgaaaa
     3421 cgtgcgtatg tacgtctgcg gtatgaccgt ttacgattac tgccatttgg gacacgcccg
     3481 cgtgatggtg gtgttcgaca tgattgcccg ttggttgcgc gagtgcggct atccgctcac
     3541 ttatgtacgc aacatcaccg acatcgacga taaaatcatt gcccgcgcgg ctgaaaacgg
     3601 cgagaccatc ggcaaactga ctgcgcgttt catccaagcc atgcacgaag atgccgatgc
     3661 tttgggcgtg ttgcgtccgg atattgagcc gaaggcaacg gaaaacattc cgcaaatgat
     3721 tgccatgatt gaaaccctga ttcaaaacgg caaggcatat cctgccgcaa atggcgacgt
     3781 ttactacgcc gtgcgcgagt ttgccgctta cggacaattg tcgggcaaat cgttggacga
     3841 cctgcgtgcg ggcgaacgcg tggaagtgga cggtttcaaa cgcgatccgc ttgattttgt
     3901 gttgtggaaa gccgccaaag caggcgaacc ggcgtgggaa agcccgtggg gcaacggccg
     3961 tccgggttgg cacattgaat gctctgccat gagtgaaaac ctgttcggcg ataccttcga
     4021 catccacggc ggcggcgcgg atttgcagtt tccgcaccac gaaaacgaaa ttgcccaaag
     4081 cgtcggcgcg acggggcata cctgcggtca tcatcacgcg caaacccacc acgggcaaag
     4141 catcgccagc cacgtcaaat actggctgca caacggcttt atccgcgtgg acggcgaaaa
     4201 gatgtccaaa tcgctgggca acttcttcac catccgcgaa gtgttgaaac aatacgatcc
     4261 ggaagtcgtg cgcttcttca tcctgcgcgc ccactaccgc agcccgttga actattccga
     4321 cgcgcatttg gatgatgcaa aaggcgcgtt gacgcgtttg tatacgactt tgaaaaatac
     4381 gccgcccgcc gatccgatgc cgtctgaagc cggcgatgac tacacgcgcc gcttttatgt
     4441 tgcgatgaat gatgattttg atacggtgaa agcggtggcg gtgttgttcg aacttgccgg
     4501 cgaagtgaac aaaaccaatg acgcgcaact ggcaggccgt ctgaaagcct tgggcggcat
     4561 catcggcctg ctgcaacgcg acccgaccga gttcctgcaa ggcagcgcgg cttcagacgg
     4621 cctctccaac gaagaaatcg aagacttgat cgcccgccgc aaacaggcgc gcgccgataa
     4681 aaactgggcg gaatccgacc gcatccgcga ccttctgaac gaacacaaaa tcattttgga
     4741 agacaacgcc ggcggcacga cttggcggcg cggttgaact gtatcggaaa gggcaaaagt
     4801 accgcctttt gctctttttc cgttttcaga cggcatttcc cgcttgaagc cccgccgttt
     4861 tcagcaggca gggccgaatg ggaaatgccg ggcggcgcac cgggtgctgc tttgtgtatg
     4921 ggaacattgt gagaacttat ttttattatg cttaaaatca tttccgccaa cgtcaacggc
     4981 atccgctccg cctacaaaaa aggtttttac gaatacatcg ccgcatcggg cgcggacatt
     5041 gtctgcgtgc aggaactcaa agcgcaggag gctgatttgt ctgccgatat gaaaaatccg
     5101 cacgggatgc acggccattg gcattgcgcc gagaagcgcg gttacagcgg cgtggcggtg
     5161 tacagcaaac gcaaacccga caatgtgcaa atcggtatgg gcattgaaga attcgacagg
     5221 gaagggcggt ttgtgcgttg cgatttcggc aggttgagcg ttatttcgct ttatttgccc
     5281 agcggcagca gcgcggaaga acgccagcag gtgaaatacc gtttccttga tgcgttttac
     5341 cctatgctcg aagcgatgaa aaacgaagga cgcgacattg tcgtttgcgg cgactggaac
     5401 atcgcccacc aaaacatcga cctgaaaaac tggaaaggca atcagaaaaa ttcgggcttc
     5461 ctaccggaag aacgcgagtg gataggcaag gttatccaca agctcggttg gacggatatg
     5521 tggcgcacgc tttatcccga tgtgccgggc tacacgtggt ggagcaaccg cgggcaggcg
     5581 tatgcgaaag atgtcgggtg gcgcatcgat tatcagatgg ttacgcccga acttgccgcc
     5641 aaagccttgt ccgcacacgt ttataaagat gaaaaatttt ccgaccatgc gccgctggtc
     5701 gtggagtatg actatgctgc cgaataaagt tttgggtaaa tatgattgga atgtggacgg
     5761 aaaaacagga ataggcgcgg catgggttgt cgctgcgttt atcctgccca tgttggtgtg
     5821 ggcgatattt atgttgtcgc ggatgcaagg ctggcttgcg ccgaccaagg caaacccgat
     5881 atgggcgttg gtgtggctgc tgatttgcct gccctgcctg ctgattgcgt ccaaatgttt
     5941 gggttggaaa ggctggcggc gggtggtgaa tatttttgcc tgcctgaccg tctgcgccat
     6001 attgagcgta cccgcttcgc tgctcatcgc ctttaccctc cgggacctgc tgaaataggc
     6061 ggggcggtat gcagggtttt gaactcgaag atttgacgct ttggctgata cgcgatgccg
     6121 gagaggatca gatgtggata gaccgttggg cgatcagtta tcccgtcgtg cagatgtccg
     6181 aagcgtcggc cggtcaaagc ataggggaat ggcaggcagg gcttcagacg gcatttgaac
     6241 gcatacgtgg caaatacgtc gccgttgtcg cacacggtgc gggcgcggcc gcatttttgg
     6301 cgtggctgta tcaggcagac atcctgacac ggaagaaaat tgccaacatc atccttgtac
     6361 cgcagcgtcc cgatattttt cccgacgatg cggaacacac tttccaacgc gtccgctgtc
     6421 cctgccgtgc cgcattggtt gtacccgaac acggcggcgt gccgcacggt tgggcgcaaa
     6481 aacaggcgga tttgtggaac gcccgcctgt tggtttcccc gcattcgggc agtttgaacg
     6541 gcatgctcgg cggctggcag tggggtatga agctgatgca ggaaatgttg ctggcgtgaa
     6601 cgcttgcctt atcggaattt cggctgtagc aggaagtccg caatcgggct gttttcattg
     6661 acgtgcaggc gcatcaggtt cggataaagt cgggctgccg cataccgccg gaaatggaat
     6721 cctttatttt ttacgtcggc agtgccggca gggcgttatc cggcagggtt gcaaggaaaa
     6781 tgccgtctga aacaggcttc agacggcatt tccattcaaa cagggcttac aggctgccca
     6841 acacgatacg caatacgcgg cgcagcggct cggccgcgcc ccacaagagc tggtcgccga
     6901 cggtgaacgc gctgatgtat tcgccgccca tgcccagttt gcggatgcgt ccgacaggga
     6961 cggacagcgt gccggtaact ttggcaggag tcagctcgtg gatgctggct tctttttcat
     7021 tggggatgac tttcacccag tcattcgcgc ctgccaaaat cgtttcgatt tcggaaacag
     7081 gcaggtcttt tttcaacttc agagtgatgg cttggctgtg gcagcgcatc gcgccgacgc
     7141 ggacgcacag gccgtcaatc acggttggat tgtcgctgcg gccgaggatt ttgttggttt
     7201 ccacgccgcc tttccattct tctttggact ggccgttgcc caaatccacg tcaatccacg
     7261 gaatcaggct gccggcgagc ggtacgccga agttggcttt cggatagtct tcgctgcgca
     7321 ggaaatcgga tactttgcgg tcgatgtcga gaatcgagcc ggcaggatcg gcaagttcgt
     7381 ccgccacttg ggcgtgaacc gcgcccatac cgctgatgag ttcgcgcatg tttttcgcac
     7441 ctgcgccgga agcggcttgg taggtcatgc tggttgccca ttcgaccaaa tcgttttgga
     7501 acaggccgcc caaagccatc agcatcaggg agacggtgca gttgccgccg atgtagtttt
     7561 tcacgccgtt tttgaggccg ttgtcgataa cgttgcggtt gacagggtcg aggacgataa
     7621 tcgcgtcgtc tttcatgcgc agagaagatg ccgcgtcaac ccagtagccg ttccagccgc
     7681 tgtcgcgcag ggcttggaag acggatttgg tgtaatcgcc gccttggcag gtaacgatga
     7741 tgtccatttt ggcgagttcg gcaacattgt tggcatctaa taatgtttta gccgcctgac
     7801 cgaaatcagg ggctgcgccg ccgacgttgg aagtggtaaa gaaaaacgct tcaggaatgt
     7861 gggcgaagtc gttttcttct ttcatacgct gcatcaaaac cgaaccgacc ataccgcgcc
     7921 agccgacgaa acctactttc attgtgttgc tccttgagga aaaatcgt
//
LOCUS       DAWKOG010000091         7869 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.091, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000091 DAWKOG010000000
VERSION     DAWKOG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7869
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.091"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            296..1609
                     /locus_tag="WJK66_001421"
     CDS             296..1609
                     /locus_tag="WJK66_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEZ7579127.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
     gene            1828..2003
                     /locus_tag="WJK66_001422"
                     /pseudo
     CDS             1828..2003
                     /locus_tag="WJK66_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            1999..3231
                     /locus_tag="WJK66_001423"
     CDS             1999..3231
                     /locus_tag="WJK66_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEZ7579128.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRIFVQMPQQEDSVVSLSAHFADGLHTLQADDDDDYYRVYIRTTEQGRIAV
                     MQENEYREDLAAAAARQSVLPLMILLTVWITHKAMRPVRKLSQSLEQRRINGLSALSE
                     DNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALSLQAERLNNMPL
                     PPEAARQSAVLQQSIRRNKHLLEQLLALARSQSDETPLTKTTFGLQSRFRQVLQELMP
                     LALEKRQDIGVAVGGDVEVSADETEIYTLIKTFADNAVRYTPPEGRIDLGFTDEGKYL
                     AVWVEDNGNGIPESERARVLDPFYRILGTEQQGTGLGLSIADTLAKKYGGHLELTDSR
                     RFGHGLLIRALLDKETLK"
     gene            complement(3266..4759)
                     /gene="rng"
                     /locus_tag="WJK66_001424"
     CDS             complement(3266..4759)
                     /gene="rng"
                     /locus_tag="WJK66_001424"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEZ7579129.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            4972..5229
                     /gene="grxC"
                     /locus_tag="WJK66_001425"
     CDS             4972..5229
                     /gene="grxC"
                     /locus_tag="WJK66_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEZ7579130.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            5250..5693
                     /gene="secB"
                     /locus_tag="WJK66_001426"
     CDS             5250..5693
                     /gene="secB"
                     /locus_tag="WJK66_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEZ7579131.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVEMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDADLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            5779..7821
                     /gene="recG"
                     /locus_tag="WJK66_001427"
     CDS             5779..7821
                     /gene="recG"
                     /locus_tag="WJK66_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEZ7579132.1"
                     /translation="MMSPETRKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIVAALSALTAIESGTQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAIGTHALFSDGVAFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPELNIG
                     LVHGRMKAAEKAEVMAEFAAGRLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQARETAPMLIEQNPEIVEAHLARWLSGREGYLGV"
BASE COUNT         1652 a         2056 c         2323 g         1838 t
ORIGIN      
        1 tcccgcgtgt atggcacgcc ccgcgctccg caatgatagg ggcggtttcg tttgaatcgg
       61 cgcaccggga tgaatgcccc cgcgccgtcc cgataccgat ttgccggttg ttccgtatcg
      121 gttgccgccg tgaaatgacg gacggatgca ggaatggcgg caggcgtgaa tgggaggaaa
      181 tgaccgattg acttgtttca aaataaaaaa gtagaatgca gggcttatcc cgcgtctgtg
      241 ggcggttttg cctgcacggt cggttttctg tttaaaataa aataaaggag aaatgatgct
      301 gacgtttatc ggactgctga ttatcggggt catcgtatgg ttgctgctga cggaaaaagt
      361 gtcgcccatc atcgcattaa tcttggtgcc gctgtttggg gcgttgctgg cggggtttga
      421 tgtatcccaa ttaaaagaat tttattcggg cggcaccaaa tcggtgatgc agattgtgat
      481 tatgtttatg ttttccattt tgttttttgg aatcatgaac gatgtggggc tgtttcgtcc
      541 gatgataggc ggtttgatta agctgactcg gggtaatatc gtggcagtga gtgtggggac
      601 ggtcttggtg tcggtggtgg cgcagttgga cggggcgggt gcgacgacgt ttttattggt
      661 cgtccccgcc cttttgccgc tttacaagcg tctgcatatg aatccttacc tgctgttttt
      721 gctgctaact tccagtgcgg gattgattaa ccttctgccg tggggcgggc cgactgggcg
      781 ggttgcaagc gtgttgggcg cagatgtggg cgaattgtat aaacctttgt tgacggtgca
      841 aattatcggt gtagtgttta tccttgcgct gtccctgctt ttgggtgtgc gtgaaaaaag
      901 gcggattgtc cgggagttgg gcgcgttgcc cgccgtggcg gatttgataa agccggtgcc
      961 tttgtcggaa gaagaacaaa aattggcgcg tccgaaactg ttttggtgga atgtcctgct
     1021 gtttttggcg gcgatgagcc tgcttttttc gggcatcttc ccgccgggtt atgtatttat
     1081 gctggctgca acggcggcgt tgcttttgaa ttaccgcagc ccgcaggaac agatggagcg
     1141 gatttatgcc cacgccggcg gcgcggtgat gatggcgtcc attattttgg cggcaggtac
     1201 gtttttgggg attttgaagg gtgcggggat gttggacgcg atttccaaag acattgtgca
     1261 tatcctgccg gacgcgctgc tgccttatct gcatattgcc atcggtgtgt tgggcattcc
     1321 gcttgagttg gttttgagta cggacgctta ttatttcgga ctgtttccga ttgtggagca
     1381 gattacctcg caggcgggcg tggcgcccga agcagcaggt tatgcgatgt tgatcggcag
     1441 tatcgtcggc acttttgtta cgccgctttc gccggccttg tggatgggct tgggtttggc
     1501 gaaattgtcg atgggcaaac acatccgtta ttcgtttttc tgggcgtggg gtttgtcgct
     1561 ggcgatattg gccagttcga tagcggcagg aatcgtgcct ctgccgtaaa cggcgaagcc
     1621 gtctgaagcc tgaatcgttc ctgtggtctg tcgcaggctc tgcctgcccg tgtgttgcaa
     1681 tcgcgcccca atgccgtctg aaggcagttg ccggcgcggt ttttttgtgc cgcagttttg
     1741 aaaaataccg gcaagcgtaa aggccgctaa attgcggcta attttggggt gttagaatag
     1801 gcacggttta ttttgaaggg aaagttgatg cgtgttttgc tggtggaaga cgatgcgatg
     1861 attgcggagg cggtgtcggc aagtttgaaa gacggcggct atgcggtgga ttgggtcaaa
     1921 aacggcgcgc ggctgccgct gcccagcctt atgacttgat gctgctggat ttgggtttgc
     1981 cggggcggga cggtttggat gtttgttccg ctggcaatgc ttgcaggtat gttttcctac
     2041 tacgaaacct tccacgaaac ggaagcgttg caggacgacc tgctccgtca ggcggcattg
     2101 tacgtcgcac ccgattctaa acccgaaact ttgcccgagg gcgacggcga tacgcgcatt
     2161 ttcgtgcaga tgccgcagca ggaagactct gttgtcagcc tgtccgcgca ttttgcggat
     2221 ggtctgcaca cgcttcaggc ggacgatgac gacgattatt accgcgtcta tatccgcacg
     2281 actgaacagg gacggattgc cgtcatgcag gaaaatgaat accgtgaaga tttggcggcg
     2341 gcagcggcac ggcaaagcgt gttgccgctg atgatactgc tgaccgtgtg gattacgcac
     2401 aaagccatgc gccccgtccg caaattgtcg caaagtctcg aacaacgccg aatcaatggc
     2461 ctgtctgcct tgagtgagga caatattccc agtgaaatca gagggttcgt aaccgccatc
     2521 aacctgcttt tgaaacgtgc ggatgaagac atccgccacc gtcagcggtt tgtcgccgac
     2581 gcggcacacg aattgcgtac gccgatgaca gccctttccc ttcaggcgga acggctcaac
     2641 aatatgccgc tcccgcccga agcggcgcgg cagtctgccg ttttgcagca gagcatcagg
     2701 cgcaacaaac acctgctcga acagcttttg gcactggcgc gttcgcagtc ggatgaaacc
     2761 cctctgacga aaacgacatt cggtctgcaa agccgtttcc gccaagtgtt gcaggaactg
     2821 atgccgctgg ctttggaaaa acgtcaggac atcggcgtgg cggtcggagg cgatgtcgaa
     2881 gtgtctgccg acgaaacgga aatctatacg ctgattaaaa cctttgccga caacgcggta
     2941 cgttacacgc cgcccgaagg caggatagat ttgggtttca cggacgaagg gaaatatctc
     3001 gccgtgtggg tggaagataa cgggaacggc attcccgaat ccgaacgcgc ccgcgtcctc
     3061 gatccgtttt accgtatttt gggaacggag cagcagggga cggggctggg gctgtcgatt
     3121 gccgacacgc ttgccaaaaa atacggcgga catttggaat tgaccgacag ccgacgtttc
     3181 ggacacggtc tgttgatacg ggcgttgttg gacaaggaaa ccctgaaata gacagatgcc
     3241 gtctgaaggc ttcagacggc attttttaca tcagcactat atcgtattgt tcctgcgtgt
     3301 aagcggtttc gactgccaaa gaaatcggtt tgccgatgaa atctatcagc attgccaagg
     3361 attgcgattc ttcgtccaaa aacaaatcga tgacgttggg ggcggcgagg atgcggaaac
     3421 tttcggcatc gtaacggcgc gcttcgcgga cgatttcgcg ctggatttcg tagcatacgg
     3481 tttgcggcgt tttcagacgg cctctgcctt ggcaggaagg gcagggttcg cagaggactt
     3541 ggtttaagtt ttcgcgcgag cgtttgcgcg tcagctcgac cagtcccagg ctggtaaaac
     3601 cgtgcagggt aacgcgggta cggtcgaagg cgagggcttt ggcaagctcc tgcaacacgg
     3661 cttcgcggtg gctttcctgc gccatatcga tgaagtcgat gatgatgatg ccgccgaggt
     3721 tgcgcagcct caattcgcgg gcgatggtgt ggcaggcttc gaggttggtg cggaaaatcg
     3781 tttcatcgaa attgcgtgcg ccgacgaagc cgccggtgtt cacgtctatc gtggtcatcg
     3841 cttcggtgga ttcgataatc aggtagctgc cgaagttgag gttgacgcgc ggttgcaggg
     3901 cgcggctgat ttcctgttcg acgttgtggg tttcaaacag cgggcgttcg cctttgaaca
     3961 attctatcct gcccaatgcg ccgtggacgt attgttcggc aaaacgcgtc atccgtccgt
     4021 ggtttacggt ggaatcgacg aggattttct gcgtgtcgca gccgaccata tcgcgcaata
     4081 cgcgcaaact caagggcaaa tcctgataaa gcagggtttc cggcggccgg attttcgcct
     4141 gttcttggat gtgttcccac actttggtca ggtagtcgat gtcggactgg agctgttcgt
     4201 cggtggcgtt ttcggcgttg gtgcggatga tgtagccccg gcaggcattt tccggcagga
     4261 gcttgtcgag gcgttcgcgc aggctgctgc gttcggcatc gtcttcgatg cgttgggaca
     4321 cgccgatgtg gtcttcttgc ggaagatgga cgaggaaacg ccccgccagc gagatttggg
     4381 tggaaagccg cgcgcctttg gtgttgatcg ggtctttgat gacctgcacc aaaacagact
     4441 gcccttcaaa cagcatatgt tcgatgcgct gggtttcttc ggggttgcgg cgttgttcga
     4501 ggacatcgac gatgtgtaaa aacgccgcgc gttccaagcc gatgtcgata aacgcgctct
     4561 gcatcccggg cagcacgcgg cgcaccacgc ccaaatagat attgccgacc aggctgtgtt
     4621 cgctgttgcg ctcgatgtgc agctcgcaga tattgttttc ctccaacacc gctacgcgcg
     4681 tttcctgcgg cgtgatgttg accaatatcg tttcgggcgg gcgcgcgatg tctttgggga
     4741 tggggagtcc tgacaacatg gtttttcctg aaaatgtaat aaaaatattt tgcatccgtt
     4801 ccgccgcgca gcgcgggtgc aaaaatattt ttcacaatgt ctatcatact ttaaaaaaga
     4861 aactttgaca cattccgcac atgccgtttg aacgtgcatc tgttccgcac ttgccaaaca
     4921 ggggaaatgc ccctatattg attatcattg caaaactttc ggaaacccaa tatgcagacc
     4981 gttaccatgt acacaggtcc gttttgtccc tactgcgcga tggcgaaaag gctgctgcac
     5041 gcggcaggtg tcggacatat cgacgaaatc cgtgtcgatg caagccccga agcctttgcc
     5101 gaaatgcagc agctttcggg acagcgcagc gtgccgcaga ttttcatcgg cgaaacgcac
     5161 gtcggcggat ttaccgacct ctaccgcctc cagcaggaag gcgggctgga cggactgctg
     5221 aacccttaac ccccaactag gaaaacaaaa tgagcgaaga actgcaaccc gtattcagca
     5281 tcgagcgact gtatgtcaaa gacttgtctt tggaagtgcc gcacgcgccg caaatctttt
     5341 tggaacaggg cgagcccgaa gtggagatgc gcgtttccac cggcagccaa aagctggaag
     5401 acggctacta caacgtggat gtaaccgtta ccgtaaccgc caaattggat aacgagcgca
     5461 cgatgttttt gaacgaagta acccaaagcg gtattttccg tttggaaaac atccccgaag
     5521 aagatgccga cctgctgctc ggcgtggcgt gtccgaacat cctcttccct tacgcgcgcg
     5581 aagccgtttc cggtacggta acgcgcgccg gcttcccgcc cgtcctgctc gcgccgatta
     5641 attttgaggc gatttaccaa caacagcagg aagccgaagc cgccggggct tgattcctgc
     5701 ctgtcgatgc cgtctgaatc cgtttcagac ggcatttttg ttttcggata aaataggcgc
     5761 gtccaaagga atctgcctat gatgtcgccc gaaacccgaa aacagctcaa aatcaccgat
     5821 gtttccgcca agaagctcga caaactcaac ctccataccg cgtgggattt ggtgttgcac
     5881 ctgccgctgc gttacgagga cgagacgcac attatgccga ttaaggacgc gccgattggc
     5941 gtgccgtgtc aggtcgaggg ggaggttatc catcaggaag taacgttcaa accgcgcaag
     6001 cagctgattg tccaaatcgc cgacggctcc ggcagcgtcc tttttctgcg cttcatccac
     6061 ttttacgcca gccatcagaa acagacggcg gtcggcaaac gcatccgcgc cgtgggcgaa
     6121 atcaaacacg gcttttacgg cgacgagatg attcatccca aaatccgcga tgccgagggc
     6181 ggcggtttgg cggaaagcct cacgccggtt tacccgaccg taaacggttt gaaccagccc
     6241 actttgcgcc gtattattca gacggcgttg gacgttacgc cgctgcacga cacgctgccc
     6301 gatgccctgc tgtgccgtct gaagctgcca cacctcgccg aaagcctgcg tcttttgcat
     6361 tcgccgccgc cgagtttcac gattcatcag ctttcagacg gcacgctgcc tgcatggcaa
     6421 cggctcaaat tcgatgaact tttggcgcaa cagctttcga tgcgcttggc gcgacagaag
     6481 cgtatcggcg gcacggcggc ggcattgggc ggcgacggca cattgaccca agccctgcgc
     6541 caagccctgc cgtttgccct gaccgatgcg caagaaaaag ttgtttccga aatctgccgc
     6601 gatatggcgc aaacctaccc gatgcaccgc ctgctgcaag gcgatgtcgg cagcggcaaa
     6661 accattgtcg ccgccttgtc cgcgctgacc gccatcgaat ccggcacgca ggtggctgta
     6721 atggcgccca ctgaaatcct tgccgaacaa cattttatta aatttaaaca atggctcgaa
     6781 cctttgggta ttgaagttgt ccgccttttt ggcagtttgc gtaaaaaagc caaagacgaa
     6841 gccaaagcca aactcgccga cggcagcgtc aaaatcgcca tcggcacgca cgccctgttt
     6901 tcagacggcg tggcgtttca caatttgggc ttgaccattg tggacgaaca gcaccgtttc
     6961 ggcgttgccc aacgcctcgc cctcaaaaac aaagggcgtg aagtccatca gctgatgatg
     7021 tccgccacgc ccatcccgcg cacgcttgcg atgagttttt tcgccgattt ggacgtgtcc
     7081 gtcatcgacg aattgccgcc cgggcgcacc ccgattaaaa cgcgcctcgt caacaacgtc
     7141 cgccgcgccg aagtcgaagg cttcgtcctc ggcacttgcc gaaaagggcg gcaggcgtat
     7201 tgggtctgcc cattgattga agaaagcgaa accctgcaac tgcaaaccgc cgccgaaacc
     7261 ctcgcccggc ttcagacggc attgcccgaa ctcaatatcg gactggtaca cgggcgcatg
     7321 aaggccgccg aaaaggccga agtcatggcg gaatttgccg caggccgtct gaacgttttg
     7381 gttgccacca ccgtcatcga agtcggcgta gatgtgccca atgccgccct gatggtcatc
     7441 gaacacgccg agcgcatggg cttggcgcag ctgcaccaat tacgcggtcg ggtcgggcgc
     7501 ggcgcggcag aaagcgtgtg cgtcctcctg tttgccgaac ccttgggcga actcgccaaa
     7561 gcgcggctga aagtcatcta cgaacacacc gacggctttg aaatcgcccg ccaagacctc
     7621 aatatccgcg gcccgggcga gtttctcggc gcgcgccaaa gcggcgtgcc tatgctgcgt
     7681 ttcgccaaac tcgaagaaga cctgcacctt ttggaacagg cgcgcgaaac cgccccgatg
     7741 ctgattgagc aaaaccccga aatcgtcgaa gcgcatttgg caaggtggct ttccggcagg
     7801 gaagggtatt tgggcgtgtg agcaaaatgc cgtctgaaat attagaaaaa aaagtgtaac
     7861 ggaaatctg
//
LOCUS       DAWKOG010000092         7868 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.092, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000092 DAWKOG010000000
VERSION     DAWKOG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7868
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.092"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..1337)
                     /locus_tag="WJK66_001428"
     CDS             complement(<1..1337)
                     /locus_tag="WJK66_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HEZ7579133.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVKNAPSTTRHV
                     EEAAISDGADTLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQPPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLIDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLAGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAARLPPELNKARSHWKWSSLNTHRPETSRAERAGYVEKLQIRLSG"
     gene            complement(1411..2751)
                     /locus_tag="WJK66_001429"
     CDS             complement(1411..2751)
                     /locus_tag="WJK66_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HEZ7579134.1"
                     /translation="MLNPSRKLVELIRILDEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRDTLERVRAGSFWLWVAAATFAFFTGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAVLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLGDSSSVRLVEMLAWLPA
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIACYGILPRLLAWAVCKILLKTSE
                     NGLDLEKPYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWAVMLETEWQDGE
                     WFEGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALTECGAAWLEPDRAAQEGRLKDQ"
     gene            2894..3520
                     /locus_tag="WJK66_001430"
     CDS             2894..3520
                     /locus_tag="WJK66_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEZ7579135.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLALMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIAIYVVMGWMVLAVMKSLTASLPLAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(3584..5002)
                     /locus_tag="WJK66_001431"
     CDS             complement(3584..5002)
                     /locus_tag="WJK66_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ7579136.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWVSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIIALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDYIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFALTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYQYEFHEFHDKG"
     gene            5329..6057
                     /locus_tag="WJK66_001432"
     CDS             5329..6057
                     /locus_tag="WJK66_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEZ7579137.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(6116..6604)
                     /locus_tag="WJK66_001433"
     CDS             complement(6116..6604)
                     /locus_tag="WJK66_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEZ7579138.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCTAIVLMCRPRRRAGGLFGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWSLFDWFEPVVRGFGNCAEPDYLLGVALPVWSAAYFLAVVLTVWWAWAR
                     AK"
     gene            complement(6601..6807)
                     /locus_tag="WJK66_001434"
     CDS             complement(6601..6807)
                     /locus_tag="WJK66_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579139.1"
                     /translation="MCRNSNPPDVFFCGHCAGKSPFCPLSGLVYIGKTEIVRTCAGVD
                     SSLAISGYIQQNRHIGILIGVLRK"
     gene            complement(7362..7826)
                     /locus_tag="WJK66_001435"
     CDS             complement(7362..7826)
                     /locus_tag="WJK66_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEZ7579140.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
BASE COUNT         2035 a         2120 c         1970 g         1743 t
ORIGIN      
        1 tttcccgaca ggcggatttg aagtttctcc acatatccgg cgcgttcggc gcggctggtt
       61 tcggggcggt gcgtattgag cgacgaccat ttccaatgac tgcgggcttt gttgagttcg
      121 ggcgggagtc tggcggcgtc ccacggggct ttgcggctgt gcagctcgat atccgactgt
      181 gccgcgtgtc cgcgcgtttg caggacgtgg agcaaatcga gggcgcgggc ggcgagcagg
      241 gtcaaggttt cagggtcggt gtgcagggtt tggcggccag cgagtttgtc ggaaatggtg
      301 cgggtattgg gcaggatgcc gcccaaaaag ccgccgattg ccgtacccaa tccgagcgag
      361 ccgccgagtg tggcgatgtc cagccccaag ccgatgagcg cgccagttgc cgcgcccgtg
      421 ccggtgcgga tgccgtattg tttgagcaat tcgctgtcga acgggtcttg gcggaaggct
      481 tgcggcatcc agtcgccgcc gtcgatttcg ctgtggtaga aacggtaaag ggcaaacagc
      541 cgctgctgca tctgccgttc gagttggcgt atttccgcct gcatggtttg cagcacggtg
      601 gcggtatcct cgttttcgtc cacttcctgc ctgaaggcgg cggcatcaat taaaaagtcg
      661 gcgatttcgc ggcgcgcttc gccgtccagc cgctgccatt cgcgccggcg catggctgtc
      721 aggcggtcaa gtgtgctgcg ttcgggcaac atggtggcga ggttttccca caggcgtagt
      781 tcgccttcaa aatcaaaggc gacggtgtcg aaccctgcga aaacgtgcag gtttctcctc
      841 gccagcatgg ttgtccacga ttcgggaggc tgtccgccgg taaagttgaa cacgggcata
      901 accggtttgg cacaccatga aaggatggtc aactcgtccc tgtatttgtc gaggacgggt
      961 tcgcgcgcgt cgatgacgta cattgccata tcgctttgca agacttgccg taagactttg
     1021 gcttcctgat tgaaatcatg gtgcgcgccg tggctgccga gaaactgttg cagccgttcg
     1081 atgccgtctg aacgattgtc cgtatggttt tccagccatt ccagcacgcc gcccgcgtct
     1141 tcgagtccgg gcgtgtcgta caggaaaacc agcgtgtctg cgccgtcgct gatggcggct
     1201 tcttcgacat gacgcgtggt cgatggggcg tttttgactt cgccgaaacc gctgtcgcgc
     1261 aaaagggtac gcaggagcga ggttttgccg gtgttggtgt gtccgacgac ggcgagggaa
     1321 aggggttgtt tgttcatgat gtttttgaag aatggatttt cagacggtct tttttcagaa
     1381 tggcggctta acagaacatt tcaagtgagt ttattggtct ttcaaacgcc cttcctgcgc
     1441 cgccctgtca ggctcaagcc acgccgcgcc gcattcggtc agcgcgttac gccaatgttc
     1501 cagcttttcc gaaaggtcgt ctgaaagccc ctgttccgcc aaaagctgca ccaccgcgcc
     1561 gccctgcgcc gcttccgaaa gtcggacgat ctgccgcaac acgccgcggt cgggcacagt
     1621 ttgggcgcgc acgccgataa gcagttgcgc cggtttctgc ttcagctctg tctccagcgc
     1681 ggcaacctgt tcccgattgg cggcaacgcc cttatccagc cattcctgcg ccagcctgcc
     1741 ctcgaaccat tcgccgtcct gccattcggt ctccagcatg accgcccatt tcggcgcatc
     1801 gttcaagacg attttcggcg aaacggcgga cacggtttcc cgacgcgtat ccgcatcggt
     1861 gattttgttc tgccagcggc ggatgaccgc ctgataatag ggtttttcca aatccaatcc
     1921 gttttcgctt gttttcaaaa ggattttgca taccgcccaa gccaagaggc gcggcaggat
     1981 gccgtagcag gcgatactgc cgaccagcag ccccgaccaa gcccgcgcat cggcaatatt
     2041 gccgttcaga cggccttcga tgaccgcccg cgcatcaggc acgggaaaac ccagtttcgc
     2101 aggcagccat gccaacattt ccaccagccg taccgaagac gaatcgccca acagcgtgct
     2161 ttcccagttg aacgtatatt gccgcaccaa aagcagcaac aataccgaca ccagcattcc
     2221 gagcagcgtg cagagccaca ggctgtgcga cgttgcgcct attttccaac gtaccgaagg
     2281 ttgccgccac tcgtccgcat acagccgcaa caccgcctga tttacagggt ctttgccccg
     2341 aaaccacgtc gccggactgc tgaaaaaacg ccccactttc acgcgcagga acaacattgc
     2401 caaccatact gccagcatca gcgtattcat gcccaacacg cccgccaaaa ccaaaaagaa
     2461 attcagaccc tgattgtcca ttagaagata agtgcctgaa aaaccggtaa aaaacgcaaa
     2521 cgtcgccgcc gccacccaca accagaacga ccccgcacgc acacgttcca atgtgtcccg
     2581 cagcatacgg ttcctgtcga tcatctccgc ccgacggatg attttttcct ccgtactgcc
     2641 gtccacgcgg cgcaaagcct ccgtcgcctg cacgggatcg ccgctgaaaa taaaaccgcc
     2701 ttcgtccaaa atacggatca gctcaaccag ttttcgggat ggattcaaca taaaatgccg
     2761 tctgaaaata aaaaacagat tttaacacac gcattttcaa gaatattcac agtgtaggca
     2821 aagagtaaat ctcacacaga agcaaaagta tcggcgtaaa ctgactgcct ctactttccc
     2881 gaaagattgt gcgatgtata caggcgaacg cttcaatact tacagccatt tgagcggttt
     2941 gattctggcg gcggcaggtt tggcgctgat gctgctgaaa accataggac acggggacgg
     3001 ctaccgtatc ttcagcgtat cggtttacgg catcagcctt cttctgctct atttgagttc
     3061 ctcgctgtac cacggaattg cagccggaaa actgaaaagc attttgaaaa aaaccgacca
     3121 ctgcatgatt tatgtgctga ttgccggaag ctacacaccg tttgcactgg tttctttgag
     3181 aaacgggccg ggctggacgg tattttcact gtcctggctg ctggcggctg caggaatcgc
     3241 acaagaactc accatcggac ggaaaagcga aaaacgactg ctgtctattg cgatttatgt
     3301 cgtcatgggt tggatggtct tggcggtaat gaaatccctg acagcctcac tcccgctggc
     3361 aggactggct tggctggcgg caggcggtat gctgtacagc gtcggcattt actggtttgt
     3421 aaacgatgaa aaaatccgac acgggcacgg aatctggcat ctgttcgtat tgggcggcag
     3481 catcacccaa tttgtcagtg tgtacggtta tgtaatctga atgccgtctg aaaagcaaaa
     3541 cctcccgatc ctgaagattg ggaggttttc tgtttgccgg acatcagccc ttgtcgtgga
     3601 actcgtgaaa ttcatactga taggacaaat cccgacccgc ttttttctgt gccaaataat
     3661 catcataaat ggcacggatt tccttacgca acaaaaacag ggctatcaag ttcgggataa
     3721 ccataaagcc gttgaacata tcggacaggc tccaaaccaa atcgactttg ccgagcgtac
     3781 ccaaaacgat ggcaagcaga accaatgcgc gatagatgcc caagtgtctt cccctgaaaa
     3841 gaaaacggat gttggactca ccgaaataat accagccgat aatggtcgtc aaggcgaaaa
     3901 aggtcagaca cacggcaagc aattgcgaac cgaagcccgg aaatgccttg ttaaaggcaa
     3961 actgggtaac cgccgcgccc tgttcgcccg aaaggttggc atcagtcagc aggataatca
     4021 atgccgtagc cgtacatacc aaaatcgtat cgataaacac accgacaaat gccgccatac
     4081 cttgctgcac aggatgcttc acatcagcgg ttgcatgtgc atgcggggta gaacccatac
     4141 ctgcttcgtt ggaaaacaga ccgcgcgcca cgccgaaacg tatcgcttca cgcataccga
     4201 tacccgcagc accgcccaaa acggcttcag gattgaaggc ggcggtaaag atgtggttga
     4261 acatcggcac aatatagtcg gaaaattcaa acaggataac gacggcgcac aaaatataaa
     4321 caaccgccat aaacggcacg acaaactggg caatattggc aatacggttc acgccgccaa
     4381 tcacaaccat gcccgcaagg acggcaagca caataccgac tgccaaagaa ggcacatcaa
     4441 atgcaatggt aacggcagaa gcaatggagt ttgcctgtgt cgcattaccg ataaagccca
     4501 atgcgataat caacgcaatg gagaaaaagc ccgacaggaa acgcgccgcg cccctgccga
     4561 ttttcggagt cagaccgtgc gtaatataaa acgccggccc accgatgtat ttgccgtggc
     4621 tgacgacgcg gtatttctgc gccagcagtg cctccgcaaa aatcgtggac atccccaaaa
     4681 cggcagaaac ccacatccaa aaaatcgcgc ccggcccgcc tgcggtgatg gcggtcgcca
     4741 cgccggcgac gttgcccgta ccgatttgcg cggatatggc aaccgccaac gcctgaaact
     4801 gcgataaaga cttatcgtct ttatcgcctt tggcaaacaa gccgccgaat acagatttga
     4861 atcccgcacc cagcttggta atctgcggcg caccgagata cagcgtaaaa aacaggccga
     4921 tacccaaaag cgcgtaaatc agcaggtagt cccaaaggaa ccgattgact gtacccacca
     4981 gaacagacaa tatattttcc ataaaataaa ccttatctta caattaaaat gactgccttc
     5041 caaaagacat tccaataagg aaacacggcg agcagaccgt atttgccgca acagatgcct
     5101 taaattgtca acaatcgggg agaagctgcg ccatcatacc gtaaaatatc gttaattaaa
     5161 acatttcttt atttttaagc ggaaagcgga ggaaatcgct ttcagacggc atcgacaacg
     5221 gcacggcata aaacaggata ttttgggtac ttgcaactta tgttaaaatg ccgaccgtaa
     5281 aaaatctgac aaaaacagat taattatttg aaataagaaa ggaaatttat ggcaggtcat
     5341 agcaagtggg cgaatatcca gcataaaaaa gcccgtcagg atgccaaacg cggcaaaatc
     5401 ttcacccgtt taatcaaaga aatcaccgtt gcggcgcgta tgggcggcgg cgatcccggc
     5461 gcaaatccgc gcctgcgcct ggctttggaa aaagcagccg aaaacaatat gcccaaagac
     5521 aatgtgcaac gcgccatcga caaaggcacg ggcaacttgg aaggcgtgga atacatcgaa
     5581 ttgcgctacg aaggctacgg catcggcggc gcggctttga tggtggactg cctgaccgac
     5641 aacaaaaccc gcaccgttgc ggacgtacgc cacgcgttta ccaaaaacgg cggcaacttg
     5701 ggtaccgacg gctgcgtggc gttcaacttc gtgcatcagg gctatttggt attcgaaccc
     5761 ggcgttgacg aagacgcgct gatggaagcg gctttggaag ccggtgcgga agacgtggtt
     5821 accaacgacg acggttccat cgaagtcatt accgcgccaa acgattgggc gggcgtaaaa
     5881 tccgctttgg aggcggcagg ttacaaatcc gttgacggcg acgttacgat gcgcgcccaa
     5941 aacgaaaccg aactctccgg cgacgatgcc gtcaaaatgc aaaaactgat tgacgcgctg
     6001 gaagacttgg acgacgtgca agacgtttac acttccgccg tattgaattt ggactgatac
     6061 gcagcacagc agacatacaa taaaatgccg tctgaacctt tcagacggca ttgatttatt
     6121 tagcccttgc ccacgcccac caaaccgtca ggacaaccgc cagaaaatac gccgcgctcc
     6181 aaacaggcaa agcaacccct aacagataat ccggttcggc acaatttccg aacccgcgca
     6241 cgacaggctc gaaccaatca aacaaagacc agcccttcaa gcggaacgtc cacggcgcac
     6301 cgcacgaagg agcggttccc ggcggcagcg actgcaacca caactgatat gccgcaacag
     6361 aaatacccgt aacggccgga atgctgataa agacagcacc gaacaaaccg cccgcccttc
     6421 ttcttggtct gcacatcagg acaattgccg tacacaatgc ggttgccaaa acgcataacc
     6481 gctgactgat acacaaaacg caaggctcca tacccaaaac atactgtgcc gccaaagaac
     6541 cggcaaatgc acagaccgaa acggcaaaca gcagccaaac ggcttttcta aataacgggg
     6601 tcattttctc aacacaccaa tcaaaatacc gatatgccga ttttgctgga tatatcccga
     6661 gatggcaagg gacgaatcga caccggcgca ggtgcggacg atttcggttt tcccgatata
     6721 cacaagacct gacaagggac aaaacggcga tttgcccgca caatgcccgc aaaaaaatac
     6781 atccggagga tttgaatttc ggcaaattta gcgaatcaaa agtttatttc aatgaaatta
     6841 tatgattttt ttgaataagc ggattgacag gtttttgaag gaatttgtta ccggatagcc
     6901 atcgggcaag ttttttgcaa aatttgaatc ggtcgggtaa attttcaaaa aataattgac
     6961 agcggataag aaacggcgga taattcccgc cgtcgagttg cttgatgcag cttgattttt
     7021 ctcctctatt tctcctttgt agacttggca cacattcaac tggatgtgtg cattttttta
     7081 tctccgcttt ttttgaaatt taattacttt tattgtttga aattccattc tttaagaaat
     7141 ttaacaagaa aaacgcttgc tttttgaccg gaaaagctgc ataattcgtt ccgttagctg
     7201 gttcgagtag tcagttaata gtttctcctc tatttctcct ttgtagactt ggcacacatt
     7261 ctattgaatg tgtgcatttt tttatctgaa gcaacaagcc tctgtgcgtg atgttgttat
     7321 gtttcattta ggtgtcaaac cgcatacccg gtctgaaata ttcaatccaa atccaaaacc
     7381 ggattttctt tgacctcctc catcacaaca taactcctac tctccgaagc ggcaggcagt
     7441 tgcaatagga tattgcctag catatcccga taggcagaca tatcgggcaa acgtacttta
     7501 atcagatagt cgtattcgcc cgacaccaag tggcattcca taatttgcgg aattttcagc
     7561 acttcttttt tgaaatcttc gaaaatattg cccgatttgg attgcagctt cagctcgaca
     7621 aaaaccaata gcggtttgcc caacagatgg ggattgaggt gggcgtgata gccggaaata
     7681 taatgttccc gctccaaacg gcgcaccctc tctgtaacgg gcgtggtgga caagcctacc
     7741 ttctcggcaa gctccgtcat cgggatgcgg gcattctgtt ggaggatctt aagaatgcgg
     7801 aaatcgattt tatctagttc tttcatttgg attgccttgt atttattatt gattttaaca
     7861 aatagagt
//
LOCUS       DAWKOG010000093         7660 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.093, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000093 DAWKOG010000000
VERSION     DAWKOG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7660
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.093"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            189..1724
                     /locus_tag="WJK66_001436"
     CDS             189..1724
                     /locus_tag="WJK66_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ7579141.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLSIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     EEK"
     gene            1721..1813
                     /locus_tag="WJK66_001437"
     CDS             1721..1813
                     /locus_tag="WJK66_001437"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HEZ7579142.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(1849..3093)
                     /gene="lysA"
                     /locus_tag="WJK66_001438"
     CDS             complement(1849..3093)
                     /gene="lysA"
                     /locus_tag="WJK66_001438"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEZ7579143.1"
                     /translation="MTLFCEQVPYPRLAEAFGTPLYVYSQSALTEAFEHYQTAFAELS
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAARLGKTAPVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYQNEKVPDLGAYAQAVQKLIGTRRLKLILEPGRSLVGNAGA
                     LLTRVEFVKYGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKDIEPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVNGGGYKLIRRR
                     ETLEQQMANELACLQAEHQNAV"
     gene            complement(3104..3274)
                     /locus_tag="WJK66_001439"
     CDS             complement(3104..3274)
                     /locus_tag="WJK66_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ7579144.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDKARFGVIQTGLQL
                     QGKPQSAPPTQK"
     gene            3345..3668
                     /gene="cyaY"
                     /locus_tag="WJK66_001440"
     CDS             3345..3668
                     /gene="cyaY"
                     /locus_tag="WJK66_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEZ7579145.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAGD
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDSRDFYDVLNEALSAASGEAV
                     EIEEL"
     gene            3699..4118
                     /locus_tag="WJK66_001441"
     CDS             3699..4118
                     /locus_tag="WJK66_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HEZ7579146.1"
                     /translation="MAQLPLYRTAEIGNFTVGTPKVLESFSKHIPYGVVFEDDGDTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNHVLILWDDACTIAALCVNDYIHAVYDFVEQ
                     AGYCRNGFPEAGGEWVKVENRVLDDELLDKILSREPT"
     gene            4140..4646
                     /gene="luxS"
                     /locus_tag="WJK66_001442"
     CDS             4140..4646
                     /gene="luxS"
                     /locus_tag="WJK66_001442"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEZ7579147.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCVPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVLNVKDQSKIPELNEYQCGTYQMHSLAEAQQIAQNVLARKVAVNKNEELT
                     LDEGLLNA"
     gene            4792..7587
                     /gene="polA"
                     /locus_tag="WJK66_001443"
     CDS             4792..7587
                     /gene="polA"
                     /locus_tag="WJK66_001443"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEZ7579148.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSGETLD
                     IEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVIEHAS
                     EIKGKVGENLRAALPRLPLSYDLVTIKTDVDLHAELSDGLESLRRTAPKWAQLVVDFK
                     RWGFRTWLKEAESRMHEAADGDLFGSDTIGEQAALDMETSSERLPEKAVAPEKLDYQA
                     VTTEAQFATLLDKLSQAEKIGIDTETTSLDAMNAALVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGHLKPYLENPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYI
                     IESHLGHGLDELSERWLGLETITYESLCGKGAKQIGFADVAIGQATEYAAQDADFALR
                     LEAHLRAQMDEKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLE
                     QEAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPDMISPRDNRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPPGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGTAPENVSPEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGFVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMIDVSRWLVSDDLKSKLIMQVHDELVLEVVETELDFVKEKLPQIMAKVGGGLLD
                     VPLVAEVGVGENWEEAH"
BASE COUNT         1752 a         2046 c         2097 g         1765 t
ORIGIN      
        1 atttttcgtt ttcaagcctt caccgcttgc catcagcgtt aaattttttt acgataagca
       61 catagattgt aaacaatcgg ccacaagccg gtttgttttt tcagaagaca ttatccctgt
      121 cagacgctat ttctatatat ttcgcctata atggcttgtt tttaataaat aattcaagag
      181 gtatcaacgt gtctgattcc aagacgaaag aacgcgccac attcggcacg cgccgcgcgt
      241 ttatgattgc cgccatcggg tccgccgtcg gcttgggcaa tatttggcgt ttcccctata
      301 ttgcttttga aaacggcggc ggcgcgttca tcctgcccta tctggtcgcg cttctgacgg
      361 cgggcatccc gctgctgctg ctcgattatg ccatcggcca ccgttaccgt ggttctgcgc
      421 ccttggcttt ccgccgcctc ggacgatggt ttgagccggt cggctggtgg aacgtgatga
      481 ccaatatcgt catctgcatc tattacgcgg taattatcgg ttgggcggca agctatacct
      541 attattcggt caacgccgcc tggggtgcgg atccgcaggg ttttttcttt aaggacttcc
      601 tgcaaatggc gggcccggaa gccttgggtt tggattttgt cggcaaagtc gccggtcctt
      661 tggcgggcgt gtgggttttt accgccgcca ttatggcgtt gggcgtgcaa aagggcgtgg
      721 cgcgcgcctc gtcgttcttt atgccgctgc ttttggtgat gtttttgatt atggtcggca
      781 tttcactaac cctgccgggt gcggcaaagg gcttggacgc attgtttacg cccgactggt
      841 cgaaactcgc cgattccaag gtctgggtgg cggcatacgg gcagattttc ttttcgcttt
      901 ccatctgctt cggcattatg gttacctatt cttcttattt gaagaaaaaa accgacttgg
      961 gcggaacggg gctggtggtc ggttttgcca acagcagctt tgaactgctc gcgggcatcg
     1021 gcgtgtttgc cgcattgggc tttatggcgc aggcgggcgg taaggcggtc aacgaggttg
     1081 cctcaggcgg catcggtttg gcgtttatcg cctttccgac cattatcaac caggcaccga
     1141 tgggctggct gatcggcata ttgtttttcg gttcgctggt attcgccggc gttacgtcga
     1201 tgatttccat ccttgaagtg attgtggcgg cgattcagga caagctgaac atcgggcgcg
     1261 tcaacgccac gctgctggtc tgcattccga tgggcattgt ttccacgctg ctgttcggta
     1321 cggcgacggg gctgccggtt ttggacgtga tggacaaatt cgtcaacacc tacggcattg
     1381 ttgccgccgg ctttgtttat gttgccgcca tcatcatcag cggcaggctg ccggaattac
     1441 gcaagcacct gaacgctttg tcctccatcc gcatcggcgg cttgtggacg gtctgcgtcg
     1501 tggttaccgt cgtgatgctc ggctatatgc tgtttaaaga taccagcggc ctgatggaga
     1561 aaaattacga aggttatccg gatggtttcc tcagtatttt cggctggggg atgtcggcgg
     1621 cgttggtcgt gttcgggctg ctgctgtcgt tgctgccttg gaaacacggt caggatttca
     1681 acgtcaaaga cgaacacgaa catgaacaag gagaagaaaa atgagtactt ccgccattgt
     1741 gatgatgatt gtctcaatcg tgataatctg gggagggctg ctgctttccc tgttaaggct
     1801 gccgaacgag taagccttta gagcgttaaa aatgccgtct gaaccgcttc agacggcatt
     1861 ttgatgttcg gcttgcaggc aggcgagttc gtttgccatt tgctgttcta aggtttcgcg
     1921 tcggcggatg agtttgtatc cgccaccgtt aaccaacact tccgccgcac ggttgcgcgc
     1981 gttgtaattg ctcgccatac tggctccgta tgcgcccgcg ctgcggataa gcagcaaatc
     2041 gccttcttcg caggcgatgg tgcggtcttt gccgaggaag tcgccggttt cacaaatcgg
     2101 gccgacgatg ttggcggtca gaggctcaat gtctttggtt tcgaccgcct cgatgtgatg
     2161 ataggcatca tatagggcgg ggcgcattaa atcgttcatc gccgcatcga ccatcacaaa
     2221 gtttttctct tcgccgtatt tgacgaattc gacacgcgtc aacaacgcgc ctgcgttgcc
     2281 gaccaagctg cggccgggct caagaatgag tttcagacgg cgtgtgccga tcagtttttg
     2341 aaccgcttgg gcatacgcgc ccaaatcagg cactttttcg ttttggtaaa caatgccgac
     2401 gccgccgccc aagtccaagt gttccaacac aatgccttcg gcggcaagcg cgtcaaccaa
     2461 aatcaaaatg cgctcgcagg cttcgaccag cgggcttaag tcggttagtt gggagccgat
     2521 gtggcagtcg atgccgatga ttttcaaatt gggctgttgt gcggcgtggc ggtaggcttc
     2581 gagcgcgtcg gcgtaggcga tgccgaattt gttggctttc agacctgtgg agatgtaggg
     2641 atgggttttc gcatcgacat cgggattgac gcgcagggag acgggcgcgg ttttacccaa
     2701 acgcgcggca actttctgaa tacggtcgat ttcggggatg ctttccatat tgaagcattt
     2761 cacgcctgca ttcagcgcga actcgatttc cgcctcgctt ttgcctacgc ctgaaaatat
     2821 ggtttttgcc gcgtcgccgc ctgccgccaa aacgcgtgcc aattcgccgc cggacacaat
     2881 gtcaaaaccg ctccccaaag aagcaaagtg tttgatgatg ctcaggttgc cattggcttt
     2941 aaccgcatag cagacgagcg gggagagctc ggcaaaggca gtttggtagt gttcaaatgc
     3001 ttcggtcagc gcggattggc tgtacacata aagcggtgtg ccgaatgctt cggcaaggcg
     3061 ggggtagggg acttgttcgc aaaatagggt catattttcg ttttcatttt tgggttggag
     3121 gggcggattg cggtttgcct tgaagctgca aaccggtttg gattacgccg aaacgcgcct
     3181 tgtcgccttc tttgggcagg tagaggtcgc ctttgtaacc gcaggccgag agcaggaggg
     3241 cggttgccgc cgcaaaaaat acgccgtatt tcatcggtaa acttccttca taagcgcgaa
     3301 tgtggcaaga ttcggcatct taaacaaaaa acacgcaaaa agctatgatg accgaaagcg
     3361 agtttatccg cgcgagcgaa gcattatttg aacacatcga agaccaaatc gacgaaaacg
     3421 gctgggattt cgactgccgg tttgccggaa acgtcctgac catcgaagcc ggagacggca
     3481 cgcaaatcat cgtcaaccgc cacacgccca atcaggaatt gtggattgcc gcaaaaagcg
     3541 gcggctacca tttcgccgaa caaaacggca aatggctggc aacgcgcgac agccgcgatt
     3601 tttatgatgt tttgaacgaa gcactgagcg cggcttcggg cgaagcggtt gagattgagg
     3661 aattgtgatt caatttgata taaaaagatt gttacccaat ggcacaatta cctctatatc
     3721 ggactgccga aatcggaaat tttactgtcg gcacgcctaa agttttagaa tcattttcca
     3781 aacatatccc ttatggtgtc gtctttgaag acgacggcga cacgggctac ttctatgctg
     3841 cttcgcaaga cgggatttta gatgccttgc acatctacaa tgtcgaagat gtatccgaca
     3901 aacatatccc caatcatgtc ttgattttat gggatgatgc ctgcaccata gccgcattgt
     3961 gtgtcaacga ctacattcat gccgtctatg attttgtcga acaggcagga tattgccgca
     4021 acggcttccc tgaagcaggc ggcgaatggg tgaaagtcga aaaccgcgtc ttggatgatg
     4081 aattgctgga caaaatccta tcccgagaac ctacataacc tactcaaaag gataacaaaa
     4141 tgcccctgtt agacagtttc aaagtcgacc acacccgtat gcacgcccct gccgtacgcg
     4201 tggcgaaaac catgaccacg cccaaaggcg ataccattac cgtattcgac ctgcgctttt
     4261 gcgttcctaa caaagaaatc ctgcctgaaa aaggcataca cacgctggag catttgttcg
     4321 caggctttat gcgcgaccac ttaaacggca acggcgtcga aatcatcgac atttccccga
     4381 tgggctgccg caccggtttt tatatgagcc ttatcggcac gccttccgaa cagcaggtcg
     4441 ccgatgcgtg gctggcttcg atgcaggatg ttttgaatgt caaagaccaa agcaaaatcc
     4501 ccgagttgaa cgaataccaa tgcggcactt atcaaatgca ctcgctcgcc gaagcgcaac
     4561 aaatcgcgca aaacgtgttg gcgcgcaaag tggcggtgaa caaaaatgaa gagctgacgc
     4621 tggatgaagg gctgctgaac gcctaatccg ccaaaaatgc cgtctgaaca agggtttcag
     4681 acggcatttg ccttttccgt tataatctgg ggttgtgcgg gggcgggttt taagccggca
     4741 tcgtccttcc ctattttttt ctgtccctta tcggttttaa gcgggttttt tatgtccaac
     4801 agacctacac tcctcctcgt tgacggatcg tcctacctct accgtgcgta tcacgcgatg
     4861 gggcaaaacc tgaccgcacc cgacggtgca ccgacgggtg cgctgtatgg cgtattgaat
     4921 atgttgcgcc gtttgcggtc ggaatatccg cacgattatt gcgcggtggt ttttgatgcg
     4981 aaaggcaaaa atttccgcca tcaaatgttt gaagaataca aggcgacgcg cccgccgatg
     5041 cccgacgatt tgcgtccgca ggcggaagct ttgccggatt tggtgcgcct gacgggctgg
     5101 ccggtattgg tgattgggca ggtggaagcg gacgatgtga tcggcacgct ggcaaaacag
     5161 ggggcggaac atggtttgcg agtcattgtt tcgaccggcg ataaggacat ggcgcagttg
     5221 gtggatgagc gcgttacgct ggtgaacacg atgagcggag aaacgctgga tattgaaggc
     5281 gtgaaggcaa aattcggcgt gcgccccgac caaatccgcg attatctcgc gctgatgggc
     5341 gacaaggtgg acaacgtgcc gggcgtggaa aaatgtggcc cgaaaacggc agtgaagtgg
     5401 ctggaagcct acggctcgtt gcaaggcgtg atagaacacg cttcggaaat caagggcaaa
     5461 gtgggcgaga atctgcgtgc cgccctgccg cgcctgccgc tgtcgtatga tttggtaacg
     5521 attaaaaccg atgtggactt gcacgccgag ctttcagacg gcctcgaaag cctgcgccgt
     5581 accgcgccga aatgggcgca gctggttgtc gatttcaaac gctggggctt ccgcacctgg
     5641 ctgaaggaag cggaaagccg tatgcacgaa gcggcggacg gcgatttgtt cggcagcgat
     5701 acgataggcg aacaggcggc gttggatatg gaaacgtcgt ctgaaaggct acctgaaaaa
     5761 gccgttgccc ccgaaaagct ggattatcag gccgttacca ccgaagcgca gtttgccact
     5821 ttgttagaca aattgtcgca ggcggaaaaa atcggcatcg acacggaaac cacttcccta
     5881 gacgcgatga acgccgcgct ggtcggcatc agcatcgcgt tccaagcggg cgaagcagtt
     5941 tacatccccg taggacacag cctgaccgcc gcgccagaac agcttgattt gcaagacgta
     6001 ttaggccatc tgaaaccgta tttggaaaat cctgccctga aaaaaatcgg gcaaaacctc
     6061 aaatacgacc aacacgtttt cgccaattac ggcatcgccc tgaacggcat cgccggcgac
     6121 gccatgctcg cttcctacat catcgagagc catctcggac acggcttgga cgaattgtcc
     6181 gaacgctggc tcggcttgga aaccattacc tacgaatcgc tgtgcggcaa aggcgcgaag
     6241 caaatcggtt ttgccgatgt cgccatcggg caggcgaccg aatacgccgc ccaagacgcc
     6301 gatttcgccc tgcgcctcga agcgcacctg cgcgcgcaaa tggacgaaaa acagcttgaa
     6361 atgtatgaaa aaatggagct gcccgtcgcg caggtattgt ttgaaatgga acgcaacggt
     6421 gtgcaaatcg accgcgccga actcgcccgc caaagcgcgg aactcggcgc cgagctgatg
     6481 aagctcgaac aagaagccta cgccgccgca gggcagccgt tcaacctcaa ctcgcccaaa
     6541 cagctgcaag aaatcctgtt cgacaaaatg ggcatcccga caaaagggct gaaaaaaacc
     6601 gccaagggcg gcatttccac caacgaggcc gtgctcgaac agctcgcgcc cgactacccc
     6661 ttgcccaaaa tcatcctgca aaaccgcagc ctggcgaaac tcaaatccac ctacaccgac
     6721 aaactgcccg acatgatttc tccccgggat aaccgcgtgc ataccaccta cgcccaagcc
     6781 gtcgccatta caggacgctt ggcaagcaac aaccccaacc tgcaaaacat ccccatccgc
     6841 accgccgaag gccgccgcgt gcgccgcgcc tttaccgcgc caccgggcag cgtcatcgtt
     6901 tccgccgact attcccaaat cgaactgcgc atcatggcgc acctctctgg agacaaaacc
     6961 ctcattgccg cattccaaaa cggcgaagac gtacaccgcc gcaccgccgc cgaagtgttc
     7021 ggcactgcgc ccgaaaacgt ctcgcctgag caacgccgct acgccaaaac catcaacttc
     7081 ggcttaatct acggtatggg gcaatacggc cttgccaaat cattgggcat cgacaacctt
     7141 tccgccaaaa actttatcga ccgctacttc gcccgctacc ccggcgtcgc cgaatacatg
     7201 cagcgcacca aagaacaagc cgccgcccaa ggcttcgtcg aaaccctgtt tggccgccgc
     7261 ctctacctgc ccgacatccg caacaaaaac gccaacgccc gcgccggagc cgaacgcgcc
     7321 gccatcaacg cccccatgca aggcaccgcc tccgacctta tcaaacgcgc catgatagac
     7381 gtgtcccgct ggctggtttc agacgacctg aagagcaaac tgattatgca ggtgcatgac
     7441 gaactggtgc tggaagtcgt tgaaaccgaa ctggattttg tcaaagaaaa actgccgcag
     7501 attatggcga aagtgggcgg cggattattg gatgtaccgc tggtggctga ggttggcgta
     7561 ggggagaatt gggaagaggc acattgattg aaaggtgtta ttatgctatc tttatttaaa
     7621 taaaatttaa tttttggtat attttttcta aatgttccta
//
LOCUS       DAWKOG010000094         7637 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.094, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000094 DAWKOG010000000
VERSION     DAWKOG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7637
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.094"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            96..1886
                     /locus_tag="WJK66_001444"
     CDS             96..1886
                     /locus_tag="WJK66_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ7579149.1"
                     /translation="MAAGRISRCRYILCDFRLFDDGDPPSRNVGGGGRFSLKAFYIRR
                     IKRILPAFFAVLAATLIGGFFLFTKDDFFLLWKSALTALGFASNLYFARGKDYFDPAQ
                     EEKPLLHIWSLSVEEQFYFVFPILLLLVARKSPRVQFGFLAALCALSLAASFMPSAFD
                     KYYLPHLRACEMLVGSLTAVWMRYRQQRNLAVGRCCAAAGALFSSCILSACLFAYSEQ
                     TAYFPGPAALIPCLAVAALIYFNHYEHPLKKFFQWKITVAAGLISYSLYLWHWPILAF
                     MRYIGPDNLPPYSPAAAVVLILLLSLFSYHCIEKPFKKWQGSFAQSVLWIYALPMLIL
                     GAGSFFAMRLPFMAQYDRLGLTRSNTSCHNNTGKQCLWGDTEKQPELLVLGDSHADHY
                     KTFFDAVGKKEKWSATMVSADACAYVEGYASRVFQNWAACRAVYRYAEEHLPRYSKVV
                     LAMRWGSQMPENSRSLAYDAGFFQKFDRMLHKLSSEKQAVYLMADNLASSYNVQRAYI
                     LSSRIPGYRQTLRPDDESTLKANARIRELAAKYPNVYIIDAAAYIPADFQIGGLPVYS
                     DTDHINPYGGTELAKRFSEKQRFLNTRHNH"
     gene            1948..3243
                     /gene="tyrS"
                     /locus_tag="WJK66_001445"
     CDS             1948..3243
                     /gene="tyrS"
                     /locus_tag="WJK66_001445"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEZ7579150.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKIALYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     NQLTPFLSFEGGNAAIMANNADWFGSMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLNQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSV
                     EEIDAVEAKDQASGSKPEAQRILAEEMTRLIHGEEALVAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPTFEVSESLNVVETLVKTGLASSNKEARGFVNNKAVLLNGKPAEANNP
                     NHAAERPDDACLLTDEHKRFGKYTILRRGKRNHALLVWK"
     gene            3234..3467
                     /locus_tag="WJK66_001446"
     CDS             3234..3467
                     /locus_tag="WJK66_001446"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579151.1"
                     /translation="MEIIRLPQKCRHSRLRGNDGGGLFVSVYLVKKERFSLMLHQVWG
                     DVSTHSTAPAARFCAFGAFGGGKFAYFSRVGRA"
     gene            3543..4463
                     /gene="ribF"
                     /locus_tag="WJK66_001447"
     CDS             3543..4463
                     /gene="ribF"
                     /locus_tag="WJK66_001447"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEZ7579152.1"
                     /translation="MRIRLGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADA
                     RGLPVVAVIFEPQPKEFFAALAGKTPPCRISPLRTKLELLEGTGCVDAVWVLRFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLSGRVVHGRKLGRTLNAPTANIRLPRHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDSDCFQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMEELKRQIEADMEAAKCW"
     gene            4604..7393
                     /gene="ileS"
                     /locus_tag="WJK66_001448"
     CDS             4604..7393
                     /gene="ileS"
                     /locus_tag="WJK66_001448"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEZ7579153.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQVARQKKDFIRLGVLGDWDKPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKVSPAIDVGYPFKDTAA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     DALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGDHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYISETPRVAGMSVWEANPVILQWLEEIGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVAQRIEEKGIE
                     AWFSLDKGELLSAEDCEHYDKLSDTMDVWFDSGSTHYSVVKQREELDWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDQNGRKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYAVVLARRLQERLAGDYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSRARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKAEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFALLVSKAEVKVGSELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
BASE COUNT         1776 a         2179 c         1980 g         1702 t
ORIGIN      
        1 aacattatga gccaagcctt accctaccgc ccggacatcg acacattgcg cgccgccgcc
       61 gttttgtccg tcatcgtgtt ccatatcgaa aaaaattggc tgccgggcgg atttctcggt
      121 gtcgatatat tctttgtgat ttcaggcttt ttgatgacgg agatcctcct tcgcgaaatg
      181 tcgggggggg ggggaggttt tccctgaagg cattttatat ccgccgcatc aagcggattc
      241 tgcccgcatt tttcgccgta ttggcggcaa cgctgatagg cggcttcttt ttattcacca
      301 aagatgattt ctttcttttg tggaaatccg cgctgaccgc cttgggtttc gcctccaacc
      361 tgtattttgc aaggggcaag gattatttcg atcccgcgca ggaagaaaag cccttgctgc
      421 acatttggtc tttgtcggtc gaagaacaat tttactttgt ctttccgata ttgctgctgc
      481 ttgtcgcccg caaaagcccg cgcgtacagt tcggcttcct cgccgcattg tgcgccttaa
      541 gccttgccgc ttcctttatg ccttccgcgt tcgataaata ttacctgccc cacctgcgcg
      601 cctgcgaaat gctggtcgga tcgctgaccg ccgtgtggat gcggtaccgg caacagcgga
      661 atctcgccgt cggcagatgc tgtgccgccg ccggcgcatt gttttcctcg tgcatactgt
      721 ccgcctgcct gtttgcctat tcggaacaaa ccgcctattt cccggggccc gccgctttga
      781 ttccctgtct ggctgttgcc gcgctgattt atttcaacca ttacgaacac ccacttaaaa
      841 aatttttcca atggaaaatc accgttgccg ccggtttgat ttcctattcg ctttatctgt
      901 ggcattggcc gatattggcc tttatgcgct atatcggtcc ggataacctg ccgccttatt
      961 cgccggcggc ggcggtcgtc ctgatattgc tgctttccct gttttcttac cactgcatcg
     1021 aaaaaccgtt taaaaaatgg caaggttcgt tcgcacaatc cgttttatgg atttatgcct
     1081 tgcctatgct cattttgggg gcgggctcgt ttttcgcgat gaggctgccg tttatggcgc
     1141 aatacgaccg tttggggctg acgcgttcca acacctcctg ccacaacaat accggcaaac
     1201 aatgcctgtg gggggatacg gaaaaacagc cggagctgct ggttttgggc gactcccacg
     1261 ccgaccatta caaaacattc ttcgatgccg tgggcaaaaa agaaaaatgg tccgccacta
     1321 tggtttccgc cgacgcttgc gcctatgtgg aaggctacgc gtcccgcgtg ttccaaaact
     1381 gggccgcctg ccgcgccgtt taccgctatg ccgaagaaca cctgccccgg tattcaaaag
     1441 tggttttggc gatgcgctgg ggcagccaga tgcccgaaaa cagccgctcc cttgcctatg
     1501 atgccggttt tttccaaaaa ttcgaccgta tgctgcacaa actctcatcc gaaaaacaag
     1561 ccgtttacct gatggcggac aacttggcct cgtcttacaa cgtccagcgc gcctatatct
     1621 tgtcttctcg cataccgggt taccgccaaa ccctgcgccc ggacgacgaa agcactctga
     1681 aagccaatgc acgcatcagg gaattggcag ccaaataccc caacgtctat attattgatg
     1741 ccgccgccta tatccccgca gattttcaaa tcggcggatt gccggtttac tcggacacag
     1801 accacatcaa cccttacggc ggcacggaat tggcaaagcg tttttccgaa aaacaacggt
     1861 ttctcaatac gcgccataac cattgattcg cttaaatttg ttacaatcgg cggtttgcaa
     1921 aaacgctaat ttttttgaaa gagaccgatg agcgtcatcc aagacctgca atcgcgcggc
     1981 ctaatcgcgc aaacgaccga catcgaagcc ttagacgctt tgttgaacga acaaaaaatt
     2041 gccctttatt gcggcttcga cccgaccgcc gacagcctgc acataggaca cctgctgccc
     2101 gtattggcat tgcgccgttt ccaacaggcg gggcatacgc cgattgcttt ggtgggcggc
     2161 gcgaccggta tgatcggcga ccccagcttc aaagccgccg aacgcagcct caattccgcc
     2221 gaaaccgttg ccggctgggt agaaagcatc cgcaaccaat taaccccttt cttgagcttt
     2281 gaaggcggaa atgccgccat tatggcgaac aatgccgact ggttcggcag catgaactgc
     2341 cttgacttcc tgcgcgacat cggcaagcat ttctccgtca acgccatgct gaacaaagaa
     2401 tccgtcaaac agcgcatcga ccgcgacggc gcaggcattt ccttcaccga gttcgcctac
     2461 tccctgctgc aaggctacga ctttgccgag ttgaacaaac gccacggcgc ggttttggaa
     2521 atcggcggct ccgaccaatg gggcaacatc accgccggta tcgacctgac ccgccgcctg
     2581 aatcaaaaac aagtgttcgg cctgaccctg cctttggtta ccaaatcaga cggcaccaaa
     2641 ttcggcaaaa ccgaaggcgg cgcggtatgg ctgaacgcga aaaaaacctc gccctatcag
     2701 ttctaccaat tctggctgaa agttgccgat gccgatgtgt ataaattcct gaaatacttt
     2761 accttcctgt ctgtcgaaga aatcgatgcc gtcgaagcca aagaccaggc cagcggcagc
     2821 aagcccgaag cgcaacgcat cctcgccgaa gaaatgaccc gcctgattca cggcgaagaa
     2881 gcccttgtcg ccgcgcagcg catttccgaa agcctgtttg ccgaagacca aagcagcctg
     2941 accgaaagcg acttcgagca gctggcactc gacggtctgc cgacttttga agtttctgag
     3001 agcctcaacg tggttgagac tttggtgaaa accggtttgg cgtcttccaa caaagaagcg
     3061 cgcggctttg tgaacaacaa agcggttttg ctcaacggca aacctgccga agccaacaat
     3121 ccgaaccacg ccgccgaacg ccccgacgat gcctgcctgc tgaccgacga acacaaacgt
     3181 ttcggcaaat acaccatcct tcggcgcggc aaacgcaacc acgcgctttt ggtttggaaa
     3241 taatccgatt gccgcagaaa tgccgtcatt cccgcctgcg cgggaatgac ggcggagggt
     3301 tgtttgtctc ggtttacctg gttaaaaaag aacgattttc actgatgttg catcaggttt
     3361 ggggcgatgt ttcaacacat agcaccgcgc ctgctgcgcg tttttgtgcg tttggcgcgt
     3421 tcggcggcgg gaaatttgcc tacttttccc gcgtcgggcg ggcgtaacgg gcggcacact
     3481 gtctataaac cgcaataccg tttacaatga ccgcctgttt gaccacactc ccgaacataa
     3541 caatgagaat caggctgggg cggcacaacg cgcccgactt tccgcacggg gccgccgtaa
     3601 ccataggcaa tttcgacggc gtacacctcg gacacaaaca catcctccaa aaactccgcc
     3661 tcgaagccga cgcgcgcgga ctgcccgtcg ttgccgtcat cttcgaacct caacccaaag
     3721 aattttttgc cgccctcgca ggcaaaaccc cgccctgccg catcagcccc ctgcgtacca
     3781 aactggaatt gctggaaggg acgggctgcg tcgatgccgt ctgggttttg cgtttcgatc
     3841 aaaatttttc cgaaatatcc gcgcaagggt ttatcgaccg cctgctgcgt caaaccttga
     3901 atacgcgtta tttgctcgtc ggcgacgatt tccgtttcgg tgcggggcgg gaaggctgtt
     3961 ttgaactttt ggcacaacag cccgatatgc agaccgagcg tacgccttcc gtcatcgtcg
     4021 aagacatccg caccagcagt accgccgtgc gacaagccct ttcagacggc aaccttgcct
     4081 atgcgaaaaa acttttggga cacgactacg tcttgagcgg cagggtggtg cacggcagaa
     4141 aactcggacg caccttaaac gccccgactg ccaacatccg cctgccccgc caccgttatg
     4201 cactcggcgg cgtgttcgtc gtcgaagcgg acggcgcgtt cggcacgcgg cgcggcgtgg
     4261 cgagcttcgg cttcaatccc accgttgata gcgactgttt tcaaaagctg gaagtccacc
     4321 tgttcgactt tcaaggcgat ttgtacggac aacggttgaa cgtccgcttc ctgcacaaac
     4381 tgcgcgatga ggaaaagttt gacggtatgg aagaactgaa aaggcagatt gaagccgata
     4441 tggaagccgc aaagtgttgg tagaaaaacc ttatacaaac catccgattg ggctacaatc
     4501 aaccttttaa ctgttcagac ggcatagggg tttcccgttg tgaaatactg tttgaggcgc
     4561 aatgccgtct gaaccgaaat attgtaacaa tagagattaa aaaatgaccg attacagcaa
     4621 aaccgtaaac ctgctcgaaa gcccgtttcc gatgcgcggc aatcttgcca agcgcgagcc
     4681 tgcatggctg aaaagctggt acgagcaaaa acgctaccaa aaactgcgcg aaatcgccaa
     4741 aggtcgtccg aagttcatcc tgcacgacgg cccgccgtat gccaacggcg acatccacat
     4801 cggtcacgcc gtcaacaaaa ttctcaaaga catcattatc cgcagcaaaa cccaagccgg
     4861 ttttgacgcg ccttatgtgc cgggttggga ctgtcacggt ttgcctatcg aagtgatggt
     4921 agaaaagctg cacggcaaag acatgcccaa agcgcgtttc cgcgaattgt gccgcgaata
     4981 tgccgccgaa caagttgccc gccagaaaaa agactttatc cgtctgggcg tgttgggcga
     5041 ctgggacaag ccctacctga ccatggattt taaaaccgaa gccgataccg tgcgtatgct
     5101 cggcgaaatc tacaaatccg gctatctcta ccgcggcgcg aaaccggttc agttctgctt
     5161 ggactgcggc tcttcattgg ccgaagccga agtggaatac aaagacaaag tatcgcctgc
     5221 gattgacgtg ggctatccgt ttaaagacac cgctgcgctt gccgctgcat tcggtttggc
     5281 aggcatcgag ggcaaagcgt ttgccgtcat ctggacgacc acgccttgga cgctgccagc
     5341 gagccaagcc gtgtctgccg gtgcggacgt ggtgtatcaa ttaatcgata cgcccaaagg
     5401 caaattggtg ctggcgaaag atttggcaga agacgcgctc aaacgttacg gtttttcaga
     5461 cggcattgct attcttgccg aaaccaccgg cgacaagctg gaaaacctgc acatgaatca
     5521 tccgttcctc gaacgcgata ttcccatgct caacggcgac cacgttacca ccgacgccgg
     5581 taccggtttg gtgcatactg cgcctgctca tggtttggaa gactacgccg tctgcaataa
     5641 atacggcatc gagctttaca accccgtcaa cgccgaaggc aaatacatca gtgaaacgcc
     5701 gcgcgttgcc ggcatgagcg tttgggaggc caatcccgtt atcctgcaat ggttggaaga
     5761 aatcggcaac ctcttggcaa gcagcaaaat cgaacacagc tacgcgcact gctggcgtca
     5821 caaaacgccg ttgatttacc gtgcgaccgg ccaatggttt gtcggtatgg ataaagcagg
     5881 tgccgatggt aaaaccctgc gcgataaagc catcaaagcc gttgatgaca ctgaattttt
     5941 cccatcatgg ggtcgtgcgc gtttggaagc catgattgaa ggccgccccg actgggtggt
     6001 ttcgcgccaa cgctattggg gcacgccgat gactttcttt gttcacaaag aaacgggcga
     6061 gctgcatccg aactctgccg aacttttgga aaaagtcgcg caacgcatcg aagaaaaagg
     6121 catcgaggct tggttctccc tcgataaggg cgaattgttg agcgcggaag attgcgaaca
     6181 ttacgacaaa ctctccgaca cgatggacgt atggttcgac tccggttcga cccattattc
     6241 cgttgtgaaa cagcgcgaag aattggactg gcctgctgac ttgtacctcg aaggcagcga
     6301 tcaacaccgg ggctggtttc agtcgtccat gctgaccggc tgcgcctcat caatgggtcg
     6361 cgcgccgtac aaacagctgc tgacccatgg tttcgtggtg gatcaaaacg ggcgcaaaat
     6421 gtcgaaatcc atcggcaacg tcgtcgcgcc gcaagaagtg tataacgagt tcggcgcgga
     6481 catcctgcgc ctgtgggcgg catccaccga ttacagcggc gaattggcga tttccaaaga
     6541 aatcctcaag cgcgtaaccg aaagctaccg ccgtatccgc aacaccttga gcttcctgtt
     6601 tgccaacctc agcgacttca atcctattga agatgccgtg caacaggcag acatggtgga
     6661 aatcgaccgc tacgccgtgg tattggcgcg tcggctgcaa gagcgtctgg caggcgatta
     6721 ctatccgcgt tatgccttcc attttgccgt gaaagacatt gtttctttct gctcggaaga
     6781 cttgggcgcg ttctacctcg atatcctgaa agaccgcctc tacaccacca aagccgacag
     6841 ccgcgcccgc cgcagcgcgc aaaccgccct gtaccacatc acgcgcagtt tggttctctt
     6901 gattgcgccg attttgtgct ttaccggcga agaagcgtgg gacatcatcg gcggcggcga
     6961 agaagacagc gtcctcttcc acacctggca cgagttcccg accatcaacg aaaaagccga
     7021 agccgaactg gtgaaaaaat ggacggcaat ccgcgaagcc cgcgaagcgg taaccgccgc
     7081 catcgagcct ttgcgcgccg acaaaaccgt cggttcgtcc ttgcaagccg aagccgaaat
     7141 taccgcgccg gaagaaatgg ccggctatct gaatgctttg ggcgaagaat tgcgctttgc
     7201 tttgctggtg tctaaagcag aagtgaaagt aggcagcgaa cttgccgttg ccgctaaagc
     7261 cagtgatggt gaaaaatgcg aacgctgctg gcactacacc cgcgatgtgg gcgcggttgc
     7321 aggctatgaa accgtctgca aacgctgtgc agagaatgtc ggcggagaag gcgaaacgcg
     7381 ccattacgcc tgataaagtt tgagcaaatg ccgtctgaaa ccgccgaaca gcatttcaga
     7441 cggcattttt tgtgccgtga tttgtcttta taacggcgga ggggtttcaa gattgcggtg
     7501 ttgtcggaat gcaactgaac cgtcattccc acgaaagtgg gaatctagaa tctcgaggtt
     7561 tcagtcattt ccgatagatt cccgccgcgt cgtgggtctg gattcccgcc tgtgcgggaa
     7621 tgacggattt cgagatt
//
LOCUS       DAWKOG010000095         7556 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.095, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000095 DAWKOG010000000
VERSION     DAWKOG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7556
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.095"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            16..420
                     /locus_tag="WJK66_001449"
     CDS             16..420
                     /locus_tag="WJK66_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ7579154.1"
                     /translation="MGSLFGLHKSFGFLTLTVITLRIVWAVANRAKRPQSDSKAAAAG
                     HGILYLLMLAVPVIGMIRQYGSGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHGNLGW
                     LLFAAVVGHVAMVVVHRVQGKDVLYRMTGRVR"
     gene            complement(455..1330)
                     /gene="rsmI"
                     /locus_tag="WJK66_001450"
     CDS             complement(455..1330)
                     /gene="rsmI"
                     /locus_tag="WJK66_001450"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEZ7579155.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGKLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLARRVREVGFKVVPVVGASAVMAALSVAGVAGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPVVMFETPHRIGATLADMAELFSERRLMLAREITKTFETFLSGTVGEIQT
                     ALSADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYNLALSWKNK"
     gene            1384..1731
                     /locus_tag="WJK66_001451"
     CDS             1384..1731
                     /locus_tag="WJK66_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEZ7579156.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGAAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GSRPPEWIQNITG"
     gene            1736..2329
                     /locus_tag="WJK66_001452"
     CDS             1736..2329
                     /locus_tag="WJK66_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEZ7579157.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAGKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFNHVF
                     SKQVRALGRAGDILVGISTSGNSANVIEAIKAAHERDMHVIALTGRDGGKIAAILKDT
                     DVLLNVPHPRTARIQENHILLIHAICDCIDSVLLEGM"
     gene            2393..3001
                     /locus_tag="WJK66_001453"
     CDS             2393..3001
                     /locus_tag="WJK66_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEZ7579158.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            3009..3659
                     /locus_tag="WJK66_001454"
     CDS             3009..3659
                     /locus_tag="WJK66_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579159.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRVVLLICI
                     TAIGALAIGIVSTFNPNGGKTLQTEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRVKPQPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKEKETPKENHTKPDTPKNTPPKPHKEILDNLF"
     gene            3699..4478
                     /gene="map"
                     /locus_tag="WJK66_001455"
     CDS             3699..4478
                     /gene="map"
                     /locus_tag="WJK66_001455"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEZ7579160.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGSVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            4633..4932
                     /locus_tag="WJK66_001456"
     CDS             4633..4932
                     /locus_tag="WJK66_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ7579161.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            5170..5544
                     /locus_tag="WJK66_001457"
     CDS             5170..5544
                     /locus_tag="WJK66_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ7579162.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(5732..7198)
                     /locus_tag="WJK66_001458"
     CDS             complement(5732..7198)
                     /locus_tag="WJK66_001458"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HEZ7579163.1"
                     /translation="MTEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTFRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLRAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEDRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
BASE COUNT         1946 a         2286 c         1849 g         1475 t
ORIGIN      
        1 gaagaggcgg aatgggtggg cagcctgttc ggcctgcaca aatctttcgg tttccttacg
       61 ctgacggtga ttacattgcg catcgtgtgg gcggttgcca accgcgccaa gcgtccgcaa
      121 agcgacagca aggctgcggc ggcaggacac ggcattctgt atctgctcat gcttgccgtt
      181 cccgttatcg gcatgatccg ccaatacggc agcggccgcg gcccgttgaa agtgttcggc
      241 gttgaggtga tgcagggttc gccggaaaaa atcgagtgga tggcaaactt gggcaacacg
      301 ttccacggca atttgggctg gctgctgttt gccgccgtcg tcggacacgt cgccatggtc
      361 gtcgtccacc gtgttcaagg caaagacgtg ctgtaccgca tgacggggcg tgtccgttga
      421 ttccgttcgc actatggtgc cggtccgtcc ggcatcattt gtttttccaa gacagtgcca
      481 gattgtacaa agcttttttt ccctcgcccg tgattttggc ggcaagctcc gccgcctgtt
      541 tggtcggcag ctcggctgtg aggattttca tgatgttttg cgcggactcg gacaagcctt
      601 cgtgtttttc atcctgcgcc ggataaagca ccaacaccat ctcaccgcgc gattggttgc
      661 cgtcggcaga caatgccgtc tgaatttccc caaccgtgcc gcttaagaac gtttcaaacg
      721 ttttcgtaat ttcgcgcgcc agcattaatc ggcgttcgga gaacagttcc gccatatcgg
      781 caagcgtcgc accgatgcgg tgcggcgttt caaacatcac gacgggaaac gccgcccgca
      841 cccatttggc aaacagtttc ctgcgctctc ccgatttcgg cggtacaaaa ccgttgaaat
      901 aaaaatcgga tcccgccaca ccggccacac tcaaagccgc catcaccgcg cttgcgccga
      961 caacagggac aactttaaac ccgacctcac gcacgcggcg ggcgagtttc gcgcccgggt
     1021 cgcacacggc cggcgtaccc gcatcggaaa cctgtgccac aaccatgccg tctgaaagat
     1081 agccgacaat cttgtccgcc atctgccgtt cgttgtgttc gcgcacgctg acaagtttgc
     1141 cctgaatgcc gtacgcgctc aaaagctgcg cggtaacgcg cgtgtcttcg gcacagatga
     1201 tgtccgcctt ttgcaatacc gccaaagcgc gcagggtaat gtccgccaaa ttgccgatgg
     1261 gcgtggcaac cacgtataat gtccctccga cgacgctgtc ggaggctttc tgcaagtgtt
     1321 tctgaaacat aaaaaatgcc gtctgaaaaa caaacattat aaattataaa ggttaaaccg
     1381 attatgcgcc taaaccacaa acagggcgag gcaggggaag atgccgcgct tgccttcctc
     1441 caatcccaag gctgcacact gcttgcccgc aactggcact gcgcctacgg cgaaatcgac
     1501 ctgattgtca aaaacggcgg catgattctg tttgttgaag taaaataccg caaaaatcgg
     1561 caattcggcg gtgccgcata cagcatttcc ccatccaaat tattgaaact gcaacgaagt
     1621 gtagagtatt atctgcaaca gaacaggttg acaaacgtac cgtgccgcct cgacgcggta
     1681 ctcatcgaag gcagccgccc gcccgagtgg atacagaata ttacaggttg acgatatgac
     1741 gacattacaa gaacgcgttg ccgcccattt tgccgaaagc atccgtgcca agcaggaagc
     1801 cggaaaagta ttggtcgagc cgaccgtaca ggctgccgag ctgatgctgc aatgcctgat
     1861 gaacgacggc aagattctgg cgtgcggcaa cggcggttcg gctgccgacg cgcagcactt
     1921 cgccgccgaa atgaccggac gctttgaaaa agagcgcatg gaactcgccg ccgtcgccct
     1981 gaccaccgac acatccgcgc tgaccgccat cggtaacgac tacggcttca accacgtctt
     2041 cagcaaacag gtacgcgccc tcggacgcgc cggcgacatc ctcgtcggca tttccacttc
     2101 cggcaattcc gccaacgtca tcgaagccat taaagcggca cacgaacgcg atatgcacgt
     2161 catcgccctg accggccgcg acggcggcaa aatcgccgcc atactcaaag acaccgacgt
     2221 tctgctcaac gtcccccatc cgcgcaccgc ccgcattcag gaaaaccaca tcctgctgat
     2281 acacgccata tgcgactgta tcgactccgt actgctggaa ggaatgtaac ccttttcaga
     2341 cggcatggcg caaagcaatg ccgtctgaaa cgcccaagaa aggaagcccc cgatgaaacc
     2401 caaaccgcac accgtccgca ccctgattgc cgccattttc agccttgccc ttagcggctg
     2461 cgtcagcgca gtaatcggaa gcgccgccgt cggcgcgaaa tccgccgtcg accgccgaac
     2521 caccggcgcg caaaccgacg acaacgttat ggcgttgcgt atcgaaacca ccgcccgctc
     2581 ctatctgcgc caaaacaacc aaaccaaagg ctacacgccc caaatctccg ttgtcggcta
     2641 caaccgccac ctgctgctgc tcggacaagt cgccaccgaa ggcgagaaac agttcgtcgg
     2701 tcagattgca cgttccgaac aggccgccga aggcgtatac aactacatta ccgtcgcctc
     2761 cctgccgcgc actgccggcg acatcgccgg cgacacttgg aacacatcca aagtccgcgc
     2821 cacgctgttg ggcatcagcc ccgccacaca ggcgcgcgtc aaaatcgtta cctacggcaa
     2881 cgtaacctac gttatgggca tcctcacccc cgaagaacag gcgcagatta cccaaaaagt
     2941 cagcaccacc gtcggcgtac aaaaagtcat caccctctac caaaactacg tccaacgctg
     3001 actcggcaat gccgtctgaa ccgccttcag acggcattgc ccgacacccc aaaagcacaa
     3061 tcaaaatggc aaaaaaaccg aacaaaccct tcaggctgac ccccaaactc ctgatacgcg
     3121 tcgtattgct catctgtatc accgccatcg gcgcattggc aataggcatc gtcagcacat
     3181 tcaacccgaa cggcggcaaa accctccaaa ccgaaccgca acacaccgac agcccccgcg
     3241 aaaccgaatt ctggctgcca aacggcgtag tcggacaaga tgccgcccaa cccgaacacc
     3301 accacgccgc ctcatccgaa cccgcacagc cggacggcac agacgaaagc ggcagcggac
     3361 tgccgtcccc tgccgcaccc aagaaaaacc gggtcaaacc gcaacctgcc gacacagctc
     3421 aaaccgacag gcagccggac gacgccggaa cacaagctga aaacacactc aaagaaaccc
     3481 ccgtactgcc cacaaacgtc ccccgtcccg aaccccgaaa agaaacaccc gaaaaacagg
     3541 cgcagcccaa agaaacaccc aaagaaaaag aaacgcccaa agaaaaccat accaaaccgg
     3601 acaccccgaa aaacacgccg cccaaacccc ataaagaaat tctcgacaac ctcttctgac
     3661 ccggcacggc aggcacaccc gcaatccaag gaagcattat gaacggcatc atcatcaaaa
     3721 cccccgaaga aatcgaaaaa atgcgcgagc tgggcaaact cgtcgccgaa gccctcgact
     3781 acatcggaca attcgtcaaa cccggcgtaa ccaccgacga aatcgacaaa ctcgtttacg
     3841 actaccacgt caacgtccaa ggcggctatc ccgcccccct gcactacggc aacccgccct
     3901 accccaaatc ctgctgcacc tccgtcaacc acgtcatctg ccacggcatt cccgacgaca
     3961 agccgctcaa agaaggcgac attatcaaca tcgacctcac catcaaaaaa gacggcttcc
     4021 acggcgactc cagccgtatg tttaccgtcg gcaaagtctc ccccatcgcc caacgcctga
     4081 tcgacgtaac ccacgcctcc atgatggcgg gcatagaagc cgtcaaaccc ggcgcgacac
     4141 tgggcgacgt aggttacgcc tgccaacagg ttgccgaaaa cgccggctat tccgtcgtac
     4201 aggaattctg cggacacggc atcgggcgcg gtttccacga agccccgcaa gtgttgcact
     4261 acggaaaaaa aggacagggc tccgttctaa aaccgggtat gatttttacc gtcgaaccga
     4321 tgatcaacca aggcaaacgc cacctgcgta tcctcaacga cggctggacg gtggttacca
     4381 aagaccgctc cctctccgcc caatgggaac acgaagtctt ggtgaccgaa accggctacg
     4441 aaatcctcac cgtcagcccc gcctccggca aaccctgaag ccggacgtat ccgccccata
     4501 aaaacaaaca atgccgtctg aaagaaacgg cagatatgat atataatata aaaacaggct
     4561 cgacccggca cattacgaaa acaaagcaaa tcggaatttg ccccgcaacc agacaaactt
     4621 aaaggaagtt ttatgaaaat atttgaaaat atagaagatg ttaaagccat ccgtaaaaag
     4681 accgggctga accagataga cttctggggc aaggtcggcg ttacccagtc tggaggctcg
     4741 cgctacgaaa ccggccgcaa aatgcccaaa cccgtacgcg aactgctccg cctcgtccat
     4801 atcgaatgca tcgatttggc gaaagtcaac aaaaaagata tggaaatcgc cgccctgttg
     4861 aaaaaacacc atcccgacct gtatgccgag ttgtccaaac agaccaagtc cgaaagaaaa
     4921 aaacaaagtt aaaccgcaac ctccggatgc ccgaaggttt ttcatttccg aaaaacgcaa
     4981 acaatgccgt ctgaaacacc ggacaggccg ccgtatccca cctgccgccc ctgcctcaaa
     5041 ccgccgaatc gcccgaaccc gcctttttac aaactttatc caatttcctg tttatttcag
     5101 gatacgccga cattagaatg tcaaaccgtc cgaaacgggc aaactccaca tccatccaaa
     5161 ggaataaaaa tgaaacttct gaccaccgca atcctgtctt ccgcaatcgc gctcagcagt
     5221 atggctgctg ctgccggcac gaacaacccc accgttgcca aaaaaaccgt cagctacgtc
     5281 tgccagcaag gtaaaaaagt caaagtaacc tacggcttca acaaacaggg tctgaccaca
     5341 tacgcttccg ccgtcatcaa cggcaaacgt gtgcaaatgc ctgtcaattt ggataaatcc
     5401 gacaatgtgg aaacattcta cggcaaagaa ggcggttatg ttttgggtac cggcgtgatg
     5461 gatggcaaat cttaccgcaa acagcccatt atgattaccg cacctgacaa ccaaatcgtc
     5521 ttcaaagact gttccccacg ttaatcaggc aacaaaaaac agcgttttca gaaatgaaaa
     5581 cgctgttttt ttgaccgttc cattattcgc aaaagggaaa aaacgattac ctgccccgtg
     5641 tatcaaaacc tgccctgccg gatgaagggc ataaccggca gggacggcgt caacaccata
     5701 tgggggtacg gcttttcttg aaagattcgg cttaaatatc caatactttc gcggtatagg
     5761 cgataatttc atccgccctt tcagggtttt cgttcaactt gatgccgtaa cccggtacca
     5821 gctctttcag acggtcttcc caagacgggg cgcgctcggg gaagcattgg tgcatcagcc
     5881 ggatcatcag cggcacagcg gtcgatgcgc ccggcgacgc gcccagcaat gcggcgagtg
     5941 agccgtcggc gtgggcgaca atctccgtac caaactggag cacgccgcct ttttcggagt
     6001 cttttttaat gatttggacg cgttgccctg cggtgatgag ttcccagtcg tcggggtttg
     6061 cctcggggta gtattccagc agggaggcga agcgttcttc tttggtttta cgcaattcgc
     6121 ccagcaggta tttggtcagc ggcatattcg cccagccggc gcgcagcata ggatagaggt
     6181 tgtccatatg gatggacagc ggcaaatcca taagcgagcc ttgcttgagg aagttggaac
     6241 ggaagcctgc gtaagggccg aacataaggt ggcgtttgcc gtccacgttg cgtgtgtcga
     6301 ggtgcgggac ggacatcggc ggcgcgccga cggaagcctg cccgtacact ttggcgttgt
     6361 gttgttcggc agtttcgggg ttgctgttgc ggaagaacag gccggacacg gggaagccgc
     6421 cgtagccttt gccttcgggg atgccggatt tttgcagcag ggtcagcgcg ccgccgcccg
     6481 cgccgaggaa gaggaagcgg gtacggaagg tgagctgccc gtcggggttg cgggtatcgg
     6541 cggttttgag cacccacgcg ccgtcggatt cgcgtttgat gtcttcgacg tggcggttga
     6601 actcggtttt tacgcccttg ccctgcaaat atttcaccat ttggcgcgtc agccgtccga
     6661 aatcgacatc cgtgccttcg gcggagtagt tggcggcgac gggttggttt tcgtcccggc
     6721 cgcgcatcat cagcggagcc caatcggaaa ttttgttccg atcggtggaa aattccatat
     6781 tttcaaaaag tttttgggtt ttaaacgcgt cataacgttt ttgaagataa gaacaatggt
     6841 cttcattcat caccaaagac atatgcggca cggcattgat gaaggaattg tcttccaact
     6901 tgccttccgc gaccagcgtc gcccaaaact ggcggctgac atgaaactgt tcggcaatat
     6961 tgagggcgcg cgccggatcg ataatcccat ttgcacccaa cggcgcatag ttcaattcgc
     7021 acagcgcgga atgccccgtg ccggcgttgt tccacgcgtt tgacgattcc aacgccacat
     7081 cttccaagcg ttcaatcagg gtgatttccc aagacggttc gagttctttg agcaaaacgc
     7141 ccaaagtcgc gctcataatg ccgccgccca ccaagacaac gtctgtcgct tcagtcatgg
     7201 tttactccta aaaaacaggc atcttctgcc cttatggtta tttgccgtac tacaaacgcc
     7261 tgaatcgcaa aagcagggaa aaccggcaat ggtgtgtgtc cgagtatgct gtttcggggt
     7321 tggaatgcgt tgcaagtatg gcttccgaca ccgcttcagg ggcttgtaat atgttatcgt
     7381 gaatgtagtg gattttactg ggaaatgcaa agtttttctg tcgcccgcca agcccgggaa
     7441 ctgcgaaatg aaaaataaaa atagttattt atctatatat atcaaatttt taatagataa
     7501 aaaaccaaaa ttgtttatat attaattttt aaaagattgt cagcatattg cgttaa
//
LOCUS       DAWKOG010000096         7406 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.096, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000096 DAWKOG010000000
VERSION     DAWKOG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7406
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.096"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(151..573)
                     /locus_tag="WJK66_001459"
     CDS             complement(151..573)
                     /locus_tag="WJK66_001459"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEZ7579164.1"
                     /translation="MSIMEVEVVSSEQKIYSGEATFIVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
     gene            complement(584..1981)
                     /gene="atpD"
                     /locus_tag="WJK66_001460"
     CDS             complement(584..1981)
                     /gene="atpD"
                     /locus_tag="WJK66_001460"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEZ7579165.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            complement(2019..2894)
                     /gene="atpG"
                     /locus_tag="WJK66_001461"
     CDS             complement(2019..2894)
                     /gene="atpG"
                     /locus_tag="WJK66_001461"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEZ7579166.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            complement(2919..4466)
                     /locus_tag="WJK66_001462"
     CDS             complement(2919..4466)
                     /locus_tag="WJK66_001462"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEZ7579167.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVISVTDGIVRIYGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            complement(4477..5010)
                     /locus_tag="WJK66_001463"
     CDS             complement(4477..5010)
                     /locus_tag="WJK66_001463"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEZ7579168.1"
                     /translation="MAEFATIARPYAKALFGLAQEKSQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            complement(5015..5485)
                     /locus_tag="WJK66_001464"
     CDS             complement(5015..5485)
                     /locus_tag="WJK66_001464"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEZ7579169.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            complement(5556..5792)
                     /gene="atpE"
                     /locus_tag="WJK66_001465"
     CDS             complement(5556..5792)
                     /gene="atpE"
                     /locus_tag="WJK66_001465"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEZ7579170.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            complement(5849..6715)
                     /gene="atpB"
                     /locus_tag="WJK66_001466"
     CDS             complement(5849..6715)
                     /gene="atpB"
                     /locus_tag="WJK66_001466"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEZ7579171.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            complement(6705..7058)
                     /locus_tag="WJK66_001467"
     CDS             complement(6705..7058)
                     /locus_tag="WJK66_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HEZ7579172.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAVWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
BASE COUNT         1948 a         1969 c         1680 g         1809 t
ORIGIN      
        1 ctatatgttt cggttaggcg gcaggctgcc ctattgaata ccttaaagca ggctatgcct
       61 gccaacgcca tatccaaaca cagtctttaa tttaaatccg gaaaataaaa agcacgacca
      121 aacggtcgtg cttttccaaa ccaaacaagt ttatttcttg tgcgaacgga tatagtccaa
      181 agttttgagc tgtgcaatcg cagcagccaa tgctttatgt gcttccgcca aagccttatc
      241 gtctttagct tgggaaatgc ccgcttctgc ggcttttttc gcctcttccg cacgtgcctg
      301 atccatctcc gcactgcgga cggcaacatc cgccaagaca gttactttat caggctgtac
      361 ttccaaaaca ccgccggaaa cagcaaccaa aacctcttta tcctcgcccg gaacggtcaa
      421 acgcaaagcc cccggccgca ccaaactcat aatcggctcg tgtcgcggat aaataccgag
      481 ttcgccctgt acagtcggaa caacgataaa tgttgcctcg cctgaataga ttttctgctc
      541 gctacttacc acctcaactt ccatgatgct catgccgacc tcctcagttt aaggttttcg
      601 ctttctcaac cgcttcttcg atgctgccga ccatatagaa tgcctgctcg ggcagatggt
      661 cgtattcgcc gttcaagatg gctttgaagc cggcaatggt atcgcgcagg gcgacatatt
      721 tgcccggaga acctgtaaac acttcggcaa cgtggaacgg ttgggacagg aagcgttgga
      781 ttttacgcgc acgcattacg gtcagtttgt cttcgtcaga caattcgtcc atacccaaga
      841 tggcgatgat gtcgcgcaat tctttgtatt tctgcagggt ggactgtacg ccgcgcgcca
      901 cgtcgtagtg ttcttgaccc aagaccatcg gatccagttg gcgtgaagta gagtcaagag
      961 ggtcaactgc cgggtaaata cccaaagagg caatatcacg gctcaatacg acggttgcgt
     1021 ccaagtgggc gaacgttgtt gccggagacg ggtcggtcaa gtcgtccgca ggtacatata
     1081 cggcttggat ggaagtaata gaaccggttt gggtagaggt aatacgctcc tgcaaacgac
     1141 ccatttcttc agccaatgtc ggttggtagc ccactgcaga aggcatacgg cccaacaatg
     1201 cggatacttc ggtaccagcc agggtgtaac ggtagatgtt gtccacgaag aacaatacgt
     1261 cgcggccttt gccgttttcg tctttttcgt cacggaagta ttccgccatg gtcaaaccgg
     1321 tcaatgcgac gcgcaaacgg ttgcccggag gttcgttcat ctgaccgtaa accattgcca
     1381 ctttatccaa tacgttggaa tctttcatct cgtggtagaa gtcgttacct tcgcgggtac
     1441 gctcacccac gcctgcgaac acggacaagc cgctgtgcgc tttggcgatg ttgttgatca
     1501 attccatcat gttcacggtt ttacccacac cggcaccgcc gaacagacct actttaccgc
     1561 ctttggcaaa cggacacagc aagtcaatca ctttaatgcc cgtttcgagc aattcggttg
     1621 tggaagacag ttcgtcaaac ttaggggcgg cttggtggat ggcacggctc ttgtcggtat
     1681 caatcggacc tgcttcgtca acaggcgttc ccaatacatc gacaatgcgt cccaacgtac
     1741 ctttacctac cggcacagta atgggcgcac cggtattgct cacagtcatg ccgcgtttca
     1801 aaccgtccga gctgcccatc gcaatggtac ggactacgcc gtcgcccaaa agctgttgga
     1861 cttccaaagt cagaccgttt tcgtctaatt tcaaagcgtc gtaaacgcgc ggaatcatgt
     1921 cgcgtggaaa ttccacgtca acaaccgcac cgataatttg tacgattttg ccttggctca
     1981 ttatcgtatc ctaatttctg tacaggattc agacggcatc agacagccgc cgcacctgct
     2041 acaatttctg acaattccgt ggtaatcgca gcttgacgcg atttgttata taccagacgc
     2101 aactctttga tggcattgcc tgcattgtct gttgcagctt tcatggcaac catgcgggct
     2161 gcctgttcgg atgccatatt gtcgctcaac gcctgataaa ccacagactc taaatagcgg
     2221 cgaaccagat attccaacac tgcaagtgca gtcggttcgt agcggtattc ccagctgaac
     2281 ggtgatttgg gagctgaatc gccaatcacg ttctcaccga taggcagcaa tacttccatt
     2341 ctcggttctt gacgcatggt attgacaaaa cccgaataca ccagatggat tctgtcaatt
     2401 tcatgtttct cataccgttg gaagagttct gtcaaaggtc cgagcagcat ttccattttt
     2461 ggggtatcgc ccaaatttac ggcactggca accacattca gaccaatgct ctgacacgcc
     2521 atcagacctt tactgccaag gcatacgatt tcctcttcaa taccttgatt ccgatactct
     2581 tgaacttgtg ccaaaaactt tttcagcacg ttggcgttca aaccgccaca caaaccctta
     2641 tcagacgtaa tcaaaataaa accgacacgt ctaatctccc gatgagattc cagtaacgga
     2701 ataccatgat cggtattggt ttgcgcaaga tggctcatca ccatacgcac tttttcggca
     2761 tacggacgcg ccaaacgcat ccgttcctga gtcttccgca ttttagaggt tgacaccatc
     2821 tgcatcgctt tagtgatctt ttgggtattc tgaacactgc ggattttggt gagaatctct
     2881 tttcctactg ccatttcaga ctcctttcac ttcaagcctt atgcctgata ggcgtaagaa
     2941 gatttgaagg atttcatggc tgcttcaagc gttttctcgc tctcgtcgga cattgcacct
     3001 gaagcattga cggcttccaa aacttccgga tgttgggtac ggacaaagct caaaaattca
     3061 gattcaaaag ccagagcttt ggcaaccgga acatcagaat acgaaccgtt gttgattgcc
     3121 caaagggtca aagccatttc agccgtattc aacgtactga actgtttctg tttcatcagt
     3181 tcggttacga cttcgccatg ctccaattgt ttgcgcgtag cttcatccaa atcggatgca
     3241 aattgcgaga acgccgccaa ttcacgatat tgtgccaacg ccaaacggat accgccaccc
     3301 agctttttaa tcactttggt ttgtgcagca ccgcctacgc gggatacgga aataccggca
     3361 ttgattgcag gacggatacc ggcgttgaag aggtcggttt ccaagaaaat ctgaccgtcg
     3421 gtaatcgaaa tgacgttagt cggaacgaaa gcagatacgt cgcccgcttg ggtttcgata
     3481 atcggcaaag cagtcagaga accggttttg ccttttactt cgccgttggt caatttctcc
     3541 acttcgtgtt cattgacacg tgctgcacgt tccaacagac gggagtgcag gtagaacaca
     3601 tcgccgggat aggcttcgcg gccgggcgga cggcgcaaaa gcagggaaat ttgacggtaa
     3661 gccacagcct gtttggacaa atcgtcataa acaatcaagg catcttcgcc gcggtcgcgg
     3721 aagaattcac ccatcgtaca accggagtaa ggtgcgatat attgcaatgc cgccgcttca
     3781 gatgcagttg cagcaaccac aatcgtgtgt tccatcgcgc catgctcttc caatttgcgg
     3841 accacgttgg caatagaaga tgctttttga ccgatagcga catagataca gataacaccc
     3901 gtaccttttt ggttgacgat ggcatccagc gctacggccg ttttaccggt ttgacggtcg
     3961 ccaataatca actcacgctg accgcgaccg acaggaacca tagagtcaat cgccttcaga
     4021 ccggtttgca tcggctggtc aaccgatttg cgcgcaatca cgcccggtgc gattttttcg
     4081 ataggggcgg tcaaagttgt attaatcggg cctttgccgt cgataggccg acccaatgca
     4141 tcaacgacgc gtccgaccag ttcgcgtccg accggcactt ccaagatacg accggtacag
     4201 gtaaccgtgt cgccttcttt aatatgttcg tactcgccca acactacggc gccgacggag
     4261 tcgcgctcca ggttcatcgc caagccgaaa gtgttacccg ggaattcgag catctcacct
     4321 tgcattgcat ctgacaaacc atagatgcga acgataccgt cagttaccga aatcaccgta
     4381 ccacgggtac gcacttcggc atttacagac agattttcga tcttggcttt aatcaaatcg
     4441 ctaatttcag caggattaag ctgcatgaaa actctcctaa ttcgtcatag tcgtgtacaa
     4501 ggcactcaat ttgccttgta cagacaaatc caaaacctga tcacccactt caacttttat
     4561 gccgccaatc agctccggtt cgatttcgac agagattttc agctcgctgt cgaaacgctt
     4621 attcagcatt tgcgccaact cgccgacctg tttgtcggtc aacggatagg cactgtaaat
     4681 gacggcagat ttgatatggt tgaatgataa ggtcaagtct tgatattgag catatacttc
     4741 cggcaatatc gacaaacgtt tctgcccggc caagacgata acaaagtttt tcaactcctt
     4801 gtctttcaaa ccgaccaaat cgatgaggat atctgctttt tctgaagcat tcgtttcagg
     4861 acggtcaatc aatgaagcca ccttcccttc ctgaacaacc gccgcaagtt tttccagtcc
     4921 gcccaaccaa gactcaattt ggcttttttc ctgagccaga ccgaacaatg cctttgcata
     4981 aggtctggca atcgttgcga actctgccat aagattacag ctcctgtttc agggtatcga
     5041 gcagttttgc gtgtttggaa gcatcgactt cgctgcgcaa aatagattcg gcacctttga
     5101 cagccaacac ggcaacctgc tcgcgcaggg attcgcgtgc gcggaacaat tcctgctcca
     5161 catcggcctt tgcctgagct gcaatgcgcg ccgcctcgga agaagcctgt tctttggctt
     5221 cttcgacaat tttggcggca cgtttttcgg cgttggcaac catttcggaa acctgattac
     5281 gcccttctgc caagagttct gcaacctttt tttcagcctg ctcgaaatcg cttttaccac
     5341 gctcggcagc tgccaagcct tcggcgactt ttgcggcacg ctcatccaaa gcttttgcaa
     5401 tcggcggcca cacgaatttc atggtaaacc ataccaaacc gaaaaagacg atgatttgag
     5461 cgaataatgt tgcattgata ttcacgttac ttaaccttcg tattggggtt aatcaaacag
     5521 gctgcgcctg tacggaacgg acgaatccgt cctgattatg cacctgcaaa cgggttaacg
     5581 aaggcgaaca gcagtgcaat ggcgacacca atcaagaatg cggcatcaat caaaccggca
     5641 atcaggaaca gtttggtttg cagcggaccg atcagttcgg gctgacgggc agaagactcc
     5701 aaatatttag aaccgaccat tgcgataccg atagaggcac ccagtgcgcc caatgcaacg
     5761 atcaaaccac atgcgatagc aatcaaaccc attttaaact ccttaaagaa acaaaggtta
     5821 aactacaaaa acaaactact taggaaaatc agtgcgcatc atgtgcctgt ccgatataga
     5881 cgaacgccaa cgccatgaaa ataaacgcct gcagggtaat caccaaaata tggaaaatcg
     5941 cccatgccaa accggcaata atgtggaata caaacagaat cggatccatg acttcgacgc
     6001 tgccggaagc cgcccaagca ccgccaagca aggctatcaa caagaatacc aactcgcctg
     6061 catacatatt gccgaacaac cgcataccgt gggatacggt tttagaaaga aactcgacca
     6121 aattcagcag gaagttcgca ggtgcgagtt ttgcaccgaa cggcgcgctg aacaactcgt
     6181 gaaaccatcc gcccaatcct ttgattttga tgttgtaata gatacaaatc agcaacacgc
     6241 cgacagcgag tgccaaagtg gtgttcaaat cggcagtcgg tacgacgcgc aacagggcgt
     6301 gatggttgcc ggtaatgccc tgccatacca tcggcagcaa atcgaccggc agcatatcca
     6361 tcgcgttcat caggaaaatc cagacaaaca gcgtcagacc caacggcgcg acggcttttc
     6421 tagacttttc gttgtgaatg atgctcttac acatatcgtc cacaaactca aacaagattt
     6481 ccacggcggc ctggaaacgt ccgggaacgc ctgccgtcgc tttttttgca ccgcgccaca
     6541 acaggaagct gccgattacg cccaacagga cggcaaaaaa gacggcatca aggttaataa
     6601 acgaaaaatc agcaatgttt ttcagtccct gaccctgagt aacatccgac aaactggtca
     6661 agctctgcaa gtggtgcttg atgtagtcgg cagcggtaat ggtttcacct gccataatct
     6721 ttcactctca acaatactaa aaaaaccaaa tggctgacac cgagcagccc catcagaaac
     6781 ggggcgaaca ccagcgattg atgccataca gcaaatacgg cgagcatgga caacagcgac
     6841 agcactactt ttaaaatctc tccgaagacg aacatcctgc tttgcaggaa ggggtttccc
     6901 ctgaaaagtt ttaaaagtaa aactgcaaca aacgtgggaa gcaggtagga caaaccgcca
     6961 ccgaccgccg aaaggaatcc ggcaaaaccc catacggcaa aggcaactgc ggcgcatatg
     7021 gacaatacgg cggattgtag gatgataatc tgcttcataa agggaatgtt tccgcctcga
     7081 atttgaggcg cggctaatat aatttagaag ccttattacg tcaagcaata tgttaatctt
     7141 tgtgaaacaa cgtatcccaa tccgccgcgc tcgccgcctg aataacggcg acaggtgtca
     7201 ttctaacaca cattacatat aattacagga tattaaggag tttgtccgca atttctttac
     7261 acttttaatg ttcttacgtg atttgtttgc tttacgtgga aataataaaa aatcaacgcg
     7321 aaattgtagc agtttatcgg tcggattgtc ggcagtttgg ggaatttgct caataaataa
     7381 aaggtcgtct gaaaatattt tcagac
//
LOCUS       DAWKOG010000097         7322 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.097, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000097 DAWKOG010000000
VERSION     DAWKOG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7322
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.097"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..77)
                     /locus_tag="WJK66_001468"
     CDS             complement(<1..77)
                     /locus_tag="WJK66_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ7579173.1"
                     /translation="MRKSRLSQYKQNKLIELFVAGVTART"
     gene            complement(383..1642)
                     /gene="rho"
                     /locus_tag="WJK66_001469"
     CDS             complement(383..1642)
                     /gene="rho"
                     /locus_tag="WJK66_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEZ7579174.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEATEFLNGKIKASKNNDDFFELMRGK"
     gene            complement(1862..4246)
                     /gene="ppsA"
                     /locus_tag="WJK66_001470"
     CDS             complement(1862..4246)
                     /gene="ppsA"
                     /locus_tag="WJK66_001470"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEZ7579175.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLSERISVALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKEAMRH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRFSINGEKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKNGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITHRIAGYASPIDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGSIYEPHEENPMLGFRGAARYVAESFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            4657..5478
                     /locus_tag="WJK66_001471"
     CDS             4657..5478
                     /locus_tag="WJK66_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEZ7579176.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASEQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
     gene            complement(5718..6380)
                     /locus_tag="WJK66_001472"
     CDS             complement(5718..6380)
                     /locus_tag="WJK66_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ7579177.1"
                     /translation="MIHAVLFDLDGTLADTALDLGGALNTLLARHGLPAKSMDEIRTQ
                     ASHGAAGLIKLGAGITPDHPDYARWRTEYLEEYDRRYAQDTELFDGVNELIAELNQRG
                     IKWGIITNKPMRFTDKLVPKLGFIIPPAVVVSGDTCGEPKPSIKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYISDEDDTDSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(6393..7220)
                     /locus_tag="WJK66_001473"
     CDS             complement(6393..7220)
                     /locus_tag="WJK66_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEZ7579178.1"
                     /translation="MKPDIYALLERALLSGDPDEKGRLTDEAFAAVQNADGAEANAPP
                     ADFPRAGRPDKPVLVAPSQLTPRKMNTAEGYAAMLHAIAHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRDRLRTFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSATCGVLDIIYRDEVGHVAIGNHWYQHLCRE
                     RGLEPITLFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKNTV"
BASE COUNT         1534 a         1766 c         2143 g         1879 t
ORIGIN      
        1 gttcttgcag ttacacctgc gacaaacagt tcaatgagtt tattttgttt atactggctt
       61 agacgacttt ttctcatagg ggcaactcta acttaatttg aatttcccta gttatctagg
      121 acagccccaa atttatacaa aaatgagcgc ggttcggcgc aaccttgaat caagttcccg
      181 catcggtttt cattgccggt acggatgcgt tcaagccggt tttgcaaagg ccgcgctttc
      241 ggcaagcgga cacggacact gccgacggtt gcgccgttaa cggggggagg gaggagctgc
      301 gccgaccgtg tgaatgaaag tgccgtctga aacccgattt tcaggcttca gacggcattt
      361 cgcattaatg cgggcggcgc gtttatttgc cgcgcatcag ttcaaagaaa tcgtcgttgt
      421 ttttagaggc tttgattttc ccgtttaaga actcggtagc ttcgatttca tccatcgggt
      481 gcaggaactt gcgtaagagc cacatacgtt gcaactggtc gttcggcaca agcagctctt
      541 cgcggcgcgt gccggatttg ttgatgttga tggcggggaa gaggcgtttt tccgccatac
      601 ggcgatcaag gtgcaattcc atattgccgg tgcctttgaa ttcttcgtaa atcacatcgt
      661 ccatacggct gccggtttca accaatgcgg tggcgataat ggtcagcgaa ccgccttctt
      721 ccacgttgcg cgccgcaccg aagaaacgtt tggggcggtg cagcgcgttg gcatcgacac
      781 cgccggtcag gattttgccc gaggtgggga cgacggtatt gtaggcgcgg gcaaggcggg
      841 taatcgaatc cagcaggatg accacgtctt ttttgtgttc caccatacgc ttggcttttt
      901 caagcaccat ttcggcaact tggacatggc gttgagccgg ctcgtcaaag gtggaggaaa
      961 ccacttcgcc acggacggag cggctcattt cggttacttc ttcgggacgt tcgtcaatca
     1021 agaggacgat gagttcgact tcgggatagt ttgcggtaac ggcgtgggca atgttttgca
     1081 gcatcacggt tttaccgctt ttgggcgggg caaccaagag ggcgcgctga cctttgccga
     1141 tgggggaaat caggtcgatg gcgcgtccgg tcaggttttc ttcggacttt aagtcgcgtt
     1201 ccagcttcaa ctgttcggtc ggaaacagcg gggtcaggtt ttcaaacagg attttatggc
     1261 ggcatacttc cgggtggtcg ccgttgatgg tatcaagcct gaccagggca aaatagcgtt
     1321 cgttgtcttt tgggacgcgg acgctgcctt cgatggtgtc gcccgtatgc aggttgaagc
     1381 ggcggatttg ggtgggcgag acatagatgt cgtcggggcc ggcaagatag gacgtgtccg
     1441 cgctgcggag gaagccgaag ccgtcgggca ggatttcaag cgtgccggag caggtgaaac
     1501 cctcgccttt tttcatcatc tggcggacga tggcaaatac gaggtcttgt ttgcggaatc
     1561 ggttggcgtt ttcgatgccg tgttcttccg ccaattctaa gagtttggaa atgtgcaggg
     1621 tttgtaattc ggagacgtgc ataataatga tgtattttga agaggaaaaa gacaggcaga
     1681 tgccgtctga aagaagaagc tgaccgttgc cggttgctcg gggaaggggg aattgtaggc
     1741 agtcggcgcg tgggtgtcaa atattatcgc ggacggggca tcggcaggaa atgccgtctg
     1801 agcggagctg cttggaaaaa aatacccccg cgcttttcag gctcgggggt atgggcattg
     1861 attatttgtt caattcattc gccaaatata gccaagtttc gatgacggta tccgggttca
     1921 gggaaacgct ttcaatgcct tcctcaacca gccatttggc gaagtccgga tggtcggacg
     1981 ggccttgacc gcagatgccg acatatttgt tctgcttgcg gcaggcggag atggcaagat
     2041 gcagcatcac tttgacggca gggttgcgtt cgtcaaacga ttcggatacc aagccgctgt
     2101 cgcggtcgag accgagggtc agttgggtca tgtcgttcga gccgatggag aagccgtcga
     2161 agtattgcag gaattgttcc gccaataccg cgttgctcgg cagctcgcac atcataatca
     2221 ggcgcaggcc gtttttgccg cgttccaagc cgttttcttt cagggctttg acaacggctt
     2281 cggcttcgcc caaagtgcgg acgaacggaa tcatgatttc aacgttggtc agacccattt
     2341 cgtcacgaac gcgtttcaag gctttgcatt cgagggcgaa gcagtctttg aaactttcgg
     2401 cgacataacg cgccgcaccc cggaagccca acatcgggtt ttcttcgtgc ggttcgtaga
     2461 tgctgccacc caccaagtta gcgtactcgt tggatttgaa gtcggacata cggacgatgg
     2521 ttttacgcgg ataaaccgat gcggctaaag ttgccacgcc ttctgcgatt ttatcgacat
     2581 agaagtcaat aggagaggcg tagccggcga tgcggtgggt aatttcagct tttaactcgt
     2641 cgtcttgttt gtcaaattcc aacaaggctt tggggtggat gccgatttgg cggttgatga
     2701 tgaattccat acgcgccaag ccgatgcctt cgctcggcag gttggcgaag ctgaatgcga
     2761 gttcgggatt gccgacgttc atcatcactt tgacaggtgc tttgggcata ttgtccaagg
     2821 cgacatcggt aatctgtacg tccaacaggc cggcatagat gaagccggta tcgccttcgg
     2881 cgcaggatac ggtaacttcc tgaccgtttt tcagcaattc ggttgcattg ccgcagccga
     2941 cgacggcagg aatgcccaat tcacgcgcga taatcgctgc gtggcaggta cgtccgccgc
     3001 gattggtaac gatggcggaa gcgcgtttca tcaccggttc ccaatccgga tcggtcatat
     3061 cggtaaccag tacgtcgccg gcttcgacgg aatccatctc ggaagcatct ttaatcaggc
     3121 gtactttgcc ctgtccgact ttctgaccga tggcgcggcc ttcgcacaag acggtttttt
     3181 cgccgttgat agaaaagcgg cgcaggttgc ggctgccttc ttcttgggat ttcacggttt
     3241 cgggacgggc ttgcaggata tagagtttgc cgtccaagcc gtcgcgaccc cattcgatat
     3301 ccatcgggcg gccgtagtgt ttttcgatgg tcagcgcgta atgcgccaac tcggtgattt
     3361 cttcgtcggt aatggagaag cggttgcggt cttcttcggg gacatcgacg ttggttaccg
     3421 atttgccggc ttcggctttg tcggtaaaaa tcattttgat gtgtttcgaa cccatggttt
     3481 tacgcaggat ggcgggcttg cccgctttga gcgtaggttt gaacacataa aattcgtccg
     3541 ggttgaccgc gccttgtacg acgttttcgc ccagaccgta agaggaggta acaaagacga
     3601 cttgatcgta gccggattcg gtgtcgaggg tgaacatcac acctgatgcg ccactgtcgg
     3661 aacgcaccat gcgttgaacg ccggcggaaa gggcgacgat gtcgtgttcg aagcctttgt
     3721 ggacgcggta ggaaatggcg cggtcgttgt acagggaggc gaatacatgg cgcatcgctt
     3781 ctttaacgtt ttccaagccg ttgatgttca agaaggtttc ctgttgtccg gcaaatgatg
     3841 cgtccggcag gtcttcggcg gttgcggaag agcgtacggc aacggaaatg tccgcaccgc
     3901 cggcatcggc aaccattttg ttccatgccg cttcgatttc ggcatcgagc tgttcgggga
     3961 agggcgtgtc caaaatccat tggcggattt ctttgccgac gcgtgccagt tcggcaacgt
     4021 cttcgacatc caattttgcc agtgcgacgg aaatgcgttc gctcagaccg ttgtgtgcga
     4081 ggaatgcgcg gtaggcatcg gccgtggtgg caaagccgcc ggggacgcga acgccttttt
     4141 cggtcagctg actaatcatt tcacccagcg aggcgttttt accgcccacg cgttcaacat
     4201 ctgtcatacg caggttttca aaccagatta cgtagttgtc ggccatttgt gtgtccaatc
     4261 caaaatatgt taaaaaagaa acaaatccgc gtgcttattt taagcgattc gttcctctgc
     4321 tgtcatgtgt tttatccgtt ttaaaatcat gatgccgtct gaaaaattgc ggtttcggcg
     4381 tgtgtagcgg tttgaaactt gcagccggta tacttctttt tttgggtatt ttctttgtaa
     4441 aacaggtggt ttgaataggt taatgttttt tctgtttgat ttttttgttt attttttaaa
     4501 attttctgcc aaaaaatact ttatataaat aattttattt caaaattata ttgtgtctgt
     4561 ttgggtgtaa tccgaggtag gcgtgctgcg ggtgctttcc ttgtgtctgc cgctgctgtt
     4621 atgatgatat ttttattctg tatttaagga tggaagatga gcagtccgcg ccatgtgttt
     4681 tacatttccg accgtaccgg tctgactgcc gagaatatcg gcgaggcttt gctgaaccag
     4741 tttggcaatc tgtcgttcaa acgccatacg catccgtttg tcgatacgcc ggaaaaggcg
     4801 cgcgcggtgg tggagaaggt caatcggagc cggcaggaaa acggtcagcg tccgattgcg
     4861 tttgtcagtg tggttgatga cgaaatccgt cggattatca aaggggcgga tgcttttcag
     4921 attaatttct ttgagacttt tttgggactg ttggagaaag aactcaatac cgaagccacg
     4981 gcatccgagc aggggcatca cagtatcggt aatacgaagc gttacgatgc gcgtatggag
     5041 gcggtaaatt tctcattgaa ccacgatgac ggggtcagcg ataagaacct tcaggaagcg
     5101 gatgtaatct tgatgggtgt atcgcgttcg ggcaaaacgc cgacctgcct ttacctcgcc
     5161 ctgcaatacg gcatccgtgc ggcaaactat ccgctgattc ctgacgattt ggaatcggcc
     5221 gatctgccgc gtatggtcaa gccttatagg gataagctgt tcgggttgac catccagccg
     5281 gaacgtttgc aggccatccg ccaagagcgc cgcccgaatt caacttatgc caaaatcgat
     5341 acatgccgca gcgaggtggc agacgcgcag agtatgttca gacggcatgg gattccgttt
     5401 gcgaatacga cggataaatc agtggaagag cttgccgtac acatccttca ggcgtgcaag
     5461 ctcaaacgca ggttttgacg ggttttgatt cggtttgaag gtggaaatgc cgtctgaaat
     5521 caggtttcag acggcagttt tatagtggat taacaaaaat caggacaagg cgacgaagcc
     5581 gcagacagta caaatagtac ggagccgatt cacttggtgc ttcagcacct tagagaatcg
     5641 ttctctttga gctaaggcga ggcaacgctg tactggttta gatttaatcc actatatgtt
     5701 tgtggggcgg atatttttca gggctgtatt ttgtccagac attcgagcag atcgagcggc
     5761 gttcggatgt ggaaatccgc ctgccatgag tcggtatcgt cttcatctga gatgtagccc
     5821 cattcggcga ggacggtttt catgccggcg ttgcggcctg cttggatgtc gcgttcggca
     5881 tcaccgacgt agagtgtgtg ttgcgggtcg gcgtggattt gtccgcacgc atacagcatg
     5941 ggtttgatgc tgggcttggg ttcgccgcag gtgtcgccgc tgacgacgac ggcgggtggg
     6001 atgatgaagc cgagtttggg gacgagtttg tcggtgaagc gcatgggttt gttggtgatg
     6061 atgccccatt tgatgccgcg ttggttgagt tcggcaatga gttcgttcac gccgtcaaag
     6121 agctcggtgt cttgggcgta gcggcggtcg tattcctcca gatattcggt gcgccatcgg
     6181 gcatagtcgg ggtggtcggg ggtgatgcct gcgccgagtt tgatcagtcc tgccgcgccg
     6241 tggctggctt gggtgcggat ctcgtccatg ctttttgcag gtagtccgtg gcgagcgagc
     6301 agggtgttga gtgcgcctcc gaggtcgagg gcggtgtcgg caagtgtgcc gtcgaggtcg
     6361 aagagtacgg cgtgtatcat gtgtgttcct tttcagacgg tatttttgtt ctgtttcaaa
     6421 ccctgctcga aatcttccag catatccaat tcaaagcggc tgaagcccgc tttttcgcgc
     6481 gcttcgatgt tcacatagcc gcggaagata aacatatcgt aacgggcaat cagactgcgg
     6541 aacagggtaa tcggttccaa accgcgttcg cggcagaggt gttgatacca atggttgccg
     6601 atggcgacat gacccacttc gtcgcggtaa atgatgtcca acacgccgca ggttgccgaa
     6661 tcgccgcgct gcgccacctt cgcgcgtatc cccggcgtaa cgtccagccc gcgcgcttcc
     6721 aacacgcgcg gcaccaaagc catacgcaac agtgggtcgt aggcggtttt gtacgccata
     6781 tcccacaaat ggttgtgcgc ctcgaaactg ccgtaatcga agccgaaagt gcgcaatctg
     6841 tcgcgcatca ggcggaaatg gtacacctct tccttcgcca ctttcaccca gtcgcggaca
     6901 aactgaaacg gcagcgtgcg gaaacggtat gccgcgtcca aagccagatt gacggcgttg
     6961 aattcgatat gcgcaatcgc gtgcagcatc gccgcatagc cttctgccgt gttcattttg
     7021 cgcggggtca gttgcgacgg cgcgaccaaa acaggcttgt ccggccgtcc cgcgcggggg
     7081 aagtccgccg gtggcgcgtt tgcttccgcc ccgtccgcat tttgaacggc ggcaaacgcc
     7141 tcatccgtca gccgtccttt ttcatctggg tcgcccgaaa gcagggcgcg ttccagcaaa
     7201 gcataaatat cgggtttcat ttcaagtccg ccgtgttcgg aaaacaaata ttatagcgtt
     7261 taaaaaaaac aagatgaggc atataatctc cgcgattcgg cattccgcgc ccaaaccgtc
     7321 aa
//
LOCUS       DAWKOG010000098         7283 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.098, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000098 DAWKOG010000000
VERSION     DAWKOG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7283
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.098"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(108..2711)
                     /gene="pepN"
                     /locus_tag="WJK66_001474"
     CDS             complement(108..2711)
                     /gene="pepN"
                     /locus_tag="WJK66_001474"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEZ7579179.1"
                     /translation="MSKTVRYLKDYQTPAYRILETDLHFDIAEPQTVVKSRLTVEPQR
                     VGEPLVLDGSAKLLSVKINGAAADYMLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASAGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GEYSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDYFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPASYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNSFELTIKQTVPPTPDMADKQPMMIPVKVGLLNRNGEAVAFDYQGKRATEAVLLLT
                     EAEQTFQFESVTEAVVPSLLRGFSAPVHLNYPYSNDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIELPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGT
                     ENIDPLRYHQAHEALLDTIAVRFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLCADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALVGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
     gene            complement(2847..3641)
                     /locus_tag="WJK66_001475"
     CDS             complement(2847..3641)
                     /locus_tag="WJK66_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ7579180.1"
                     /translation="MNTILAFDIETVPDVQGIRTLYELPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQVVAISCCMRWGQDKIHIGTIGEADDGEEVVIAKFFELVEKHTPQLV
                     SWNGGGFDLPVLHYRSLIYGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWEAFHTGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWAEFVQAWK"
     gene            complement(4391..5260)
                     /locus_tag="WJK66_001476"
     CDS             complement(4391..5260)
                     /locus_tag="WJK66_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HEZ7579181.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKIADLTGLLAYLLVKPRRRIGEIN
                     LAKCFPEWSEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAGRLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDAQILKGRNRYDNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIRTATITGLSRIAVLANA
                     KVIPAIPVREADNTVTLHFYPAWKSFPGEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            complement(5311..5847)
                     /gene="ruvC"
                     /locus_tag="WJK66_001477"
     CDS             complement(5311..5847)
                     /gene="ruvC"
                     /locus_tag="WJK66_001477"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEZ7579182.1"
                     /translation="MSATVRILGIDPGSRVTGFGVIDVRGRDHFYVASGCIKTPADAP
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LASRLNPSGMQVKRGRFQ"
     gene            complement(5850..6089)
                     /locus_tag="WJK66_001478"
     CDS             complement(5850..6089)
                     /locus_tag="WJK66_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEZ7579183.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            complement(6119..7129)
                     /gene="dusB"
                     /locus_tag="WJK66_001479"
     CDS             complement(6119..7129)
                     /gene="dusB"
                     /locus_tag="WJK66_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEZ7579184.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLTS
                     DPTLRNTRKTLHRSDFVDEGGIVAVQIAGSDPQQMADAARYNVSLGAQVIDINMGCPA
                     KKVCNVQAGSALMQNEPLVAAILEAVVKAAGVPVTLKTRLGWHDDHKNLPVIAKIAED
                     CGIAALAVHGRTRTQMYKGEARYELIAETKCRLNIPVWVNGDITSPQKAQAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECAAAILNHIRAIHAFYGDTAGV
                     RIARKHIGWYIDEMPDGEQTRREINRLDSAAAQYDMLAGYLERLAEKTDSWACAYRPN
                     AF"
BASE COUNT         1618 a         1928 c         1920 g         1817 t
ORIGIN      
        1 atatgacttt gcggccgttt tgccattgcg taataaaacg atggggaagt gatgataaaa
       61 cgtgtgtgta actatatcag acggcattgt ttttctgttt gacggcctca atccaaaatt
      121 ttgccgacga tttcgcccac gtctttcgac aatccttcct gcgcccgaat gcgctgcaat
      181 gcttgtttca ccaagttttt gcggtgcggt tcgagcttat tgcagagatt gaacgcctgc
      241 actaggcggg cggcgacctg cgggttaaag cggtcgattt cgatgacttt gtcggcgatg
      301 aagcggtagc cgctgccgtc ttctgcgtga aaatgcggta cgttgcggct gaagctgccg
      361 atgagcgagc gggctttgtt ggggttttcg aggctgaact tcggatgctg caaggcggtt
      421 tgaacctgtt gcagggtgtc gctgcggcgg cttgatccaa cgagggcgaa atatttgtcc
      481 atcaccagcg cgtcgtctga aaacttgtcg gcaaactgag ccagcaggcg gttgcgcgta
      541 tcgctttcgt tgccgttaac ggcggacagg atgccccatt cgtgggtcat gttttgcgcc
      601 atttcgccgt atttttcggc aacggtttcg atgtgcgcgg ggtcggcgca caggacgaag
      661 gcgcggcaga cgttgcgcag cgtgcgccag ccggcagctt cggggctgta ctcgtagctt
      721 tggttttcct gcttcgccgc ctgacggttc aattcgtgcc atttcggcag gaagcggacg
      781 gcgattgtat ccaacaaggc ttcgtgcgcc tgatggtagc gcaacgggtc gatgttttcc
      841 gtgccgtccc acagctcggc ttcagacggc acgcccaaaa gcagggcttt gaaggcgttg
      901 tctaagaggt cgtctgaaat gactttttcg acggcggcaa gcagtttttc gtgtttcggc
      961 agttcaatac cgtctgaaag cgcggcaagg ttggcggcga cggcgcggcg gtagagcgtt
     1021 tgggcggctt cccagcgcgt gaaggcgtcg ctgtcgtggg cgagcaggag cagcaggtcg
     1081 tcgttgctgt acggatagtt cagatgcact ggcgcgctga acccgcgcag cagcgaggga
     1141 acgacggctt cggttacgct ttcgaactgg aaggtctgtt cggcttcggt cagcagtaac
     1201 acggcttcgg ttgcgcgttt gccctgataa tcgaatgcca ccgcttcgcc gttgcggttc
     1261 agcagcccga ctttgacggg aatcatcatc ggctgcttgt ccgccatatc gggcgtgggc
     1321 ggcacggttt gtttgatggt taactcgaaa ctattgtttt tcagacgacc ttccgcttcc
     1381 aagacgggcg tacccgcctg gctgtaccac aaggcgaact ggtcgagatt gatgccgttc
     1441 gcgtccgcca tcgccgcgcg gaaatcgtcg caggtcacgg cctgcccgtc gtgtcgttgg
     1501 aaatagagct tcatgccttt ttggaagccc tcttcgccga gcaaggtgtg atacatccgc
     1561 accacttccg cgcctttttc ataaacggtc atggtgtaga aattgttcat ctcctcatag
     1621 ctggcggggc gcaccggatg ggcggtcggg cctgcgtctt cggggaactg gtgctggcgc
     1681 agcaggcgga tgttctcgat gcggcgcacg gcgcggctgg cgcggtcgcc ggaaaattct
     1741 tggtcgcgga acacggtcaa cccttctttc agcgaaagct ggaaccaatc gcggcaggta
     1801 acgcggttgc ctgtccagtt gtggaaatac tcgtgtccga ccacggattc gatgccttca
     1861 aaatcggtat cggtggcggt acggctgtcg gcaaggacga acttggtatt aaaaatgttc
     1921 agccccttgt tttccatcgc gcccatattg aaatcgccca cggcgacgac catgaaaata
     1981 tccaagtcgt attccaaacc gaagcgcgtt tcgtcccact tcattgcgtt tttcagcgat
     2041 tccacggcaa agccgacctt gggcttgtcc gcttcggtgg tgtaaaactc gattttgacg
     2101 tttctgccgc tcatggtggt gaaatagtct tccgtgaccg ccaaatcgcc cgcgaccaaa
     2161 gcaaacagat aactcggttt ggcaaacggg tcttcccatt tcacccaatg gcggccgtct
     2221 gaatactcgc cgccgtcgat tttgttgccg ttggagagca aaacgggata gcgttttttg
     2281 tccgcgacga tggtggtcgt gaacttggac atgacatccg gacggtcgat atagaacgtg
     2341 attttgcgga agccctccgg ctcgcactgg gtaaacaggt taccggcgga cgcatacagc
     2401 cccatcagcg atttgttttc cgccggcagg atttcggttt ccacttcgac ggtgaagcgt
     2461 tcggacggca cgtctgcaat cgtcagcgtc tcgccttcca acatataatc cgccgccgcg
     2521 ccgttgattt tgacggacaa gagtttcgcc gaaccgtcca acaccagcgg ctcccctacc
     2581 ctctgcggct caaccgtcaa acgcgacttc acgacggttt gcggttcggc aatatcaaaa
     2641 tgcaggtcgg tttcaaggat gcggtaggca ggcgtttggt aatctttcag ataacggacg
     2701 gttttgctca tttttttctt tcaatattgt ttggtttgac tggaaaaggc ttcagacggc
     2761 ataggcaaac cctgcaccga tatgccgtat gaaaccgaaa catcagtgcg gacgcaccgt
     2821 caaacaaccg gttttaattt caatctttat ttccacgctt ggacaaattc tgcccaatgc
     2881 ggtttgtctt ctgtttgggc ggaaagatag tttctgatcc gcttgatttc catttcgtat
     2941 tcgtccgcgt cgaatctgcc gctgaccaga cagaaacgca gatacatcaa atacgtattc
     3001 acggcatcgg tttcacaata attgcggatt tccttcagcc tgcccgtatg gaatgcctcc
     3061 caaaccttgc tgccgtccat acccagcttg cccggaaaac cgcacagttt cgccatatcg
     3121 tccagcggca cgtttgccct cggctgataa agtgcgagca aatccatcaa atcgcagtgg
     3181 cgttggtggt aacggctgat gtagttgttc cacttgaaat cgcggctatc gccgaaatcg
     3241 ccgtcgccca tatcccaata gcgcgcggcg ttgatgccgt atatcaggga gcggtaatgc
     3301 agtacgggca ggtcgaaacc gccgccgttc caactgacca attgcggcgt atgtttttcc
     3361 accaactcga aaaatttggc gataaccact tcctcgccgt cgtccgcctc gccgattgtg
     3421 ccgatatgga ttttgtcctg cccccaacgc atgcagcacg aaatcgccac aacctgatga
     3481 agatgatgct gcataaaatc gccgcccgtt tgggcgcggc gtttttgctg ggcaaacagc
     3541 accacttcgt cgtcgggcag cgaggacggc agctcgtaca atgttcggat accctggaca
     3601 tcaggtacgg tttcaatatc gaaagccaaa atcgtattca tggcagtacc ttgcattcaa
     3661 aaacagactt gcgcctattg tgtcattaaa agacagataa aaaaagaggc aaccccccac
     3721 aggattgccc caatacctca aatcagagat ttacgcttca caaacaatac aggctttcgc
     3781 ctgcggcttt acccgcgtag ctcaactcta cgccggcaaa ctttcgtttc accgtttccg
     3841 atgaaacccc gaccaatcgc aagactgacc ggaaaatcct ttcaaacagc atttcctgcc
     3901 tgtcgtgtaa ttccatgtag cgaaatgtac gccattttct acgctttgcc aagcattttt
     3961 tacaatataa atgtcaaaac attaatttta taaaattgct gaaaatatta aatatatgga
     4021 tttttatttt ttatatttca ataaatataa atttaatttt gatttatatt taaatttaag
     4081 cataaaatgt caaatattaa aggaaacatg aaaggcatat attaaatatt tatttataac
     4141 tctatgtttt taaagaaaat taattttaat atattaacta gattgtctgc atatattcat
     4201 aggtttgcgg tatttcttcc aaaacctgct tcgaatttcc cgaccaagtc ttaaaaatat
     4261 tgtttttgag atacttaaat agcagcgatt atcaaatgaa atctgttcat ataatctgcc
     4321 attttgcatt taaaaaaaca atcaggagtt tcgactcgaa atgcctgata tgttttgtaa
     4381 ttttacgtag tcagtaaaaa tcggggctgc cttccggacg ggttttaaaa cgcttgtgca
     4441 gccaaaaata ctgctcggga tgttcgcgca cgcgttcctc gataaaacgg ttcatgcgct
     4501 gcgcatcggc ttgcgcgtct tcacccggaa aggatttcca agccgggtag aaatgcaatg
     4561 taaccgtatt gtccgcctcc ctgacgggga tggcgggaat cactttggca tttgcaagca
     4621 cggcaatgcg gctcaatccg gtaatcgttg ccgtccgaat accgaaaaaa tccacaaaaa
     4681 ccgaatcgtt gcgtccgaaa tcctgatcgg gcagatacag aaacggcgcg ctgcttttgc
     4741 ggaactgttt gacgagggcg cgcagccctt cggtgcgccc gatgaggaag acattgtcgt
     4801 agcggttgcg gcctttcaaa atctgtgcgt ccaatatctt gtttttttgg tgggaataca
     4861 tactaattag cgggacatcc tgattaagcg cgtacaccgc catctcgaac gcggtaaagt
     4921 gcggatacag gatgatgact ttttcccccg ccgccagcgc gtcgtccaaa taatgcttat
     4981 tgcggtagcg caccagcgat ttcaaacgcc cggcgggcgc gtaccaatat aagccgtatt
     5041 cgagcatcag tttcgccata tgtttgaaat gctgtttcaa cacggtttta cgcttttcct
     5101 cactccattc gggaaaacat ttcgccaaat tgatttcgcc gatacggcgg cgcggtttga
     5161 ccagaaggta ggcaagcaaa cccgtcaggt cggcaatctt gtgcagcagc gcaaacggca
     5221 gaaactgcaa aacatacagt acaaaaaata taaatttcat ctcgatacac attttctttt
     5281 cagacggcaa aatacaaatg ccgtctgaaa ctattgaaac cttccgcgct tgacctgcat
     5341 ccccgacggg ttgagccggg acgcaagccc gtggttgcgt aaggcgtggg tcagcgcgac
     5401 ggcaagaccg tccgccgcat ccgcctgcgg cgttcccgaa agccccagca tctgcaccac
     5461 catatgctgc acctgttctt ttgccgcctt gcctttgccg actaccgcct gtttgacctg
     5521 caaggccgtg tattccgaaa cgggcagctt atggctgacc aatgccgcca atgccgcgcc
     5581 ccgcgcctga ccgagcatca gcgtcgatgc aggattgacg ttgacgaaca cctgttccac
     5641 cgccgcctgc tgcggcttgt aaacggcaac gacttcgccg atgtgccgca caatcacggc
     5701 aatcctgtct gccagaggcg catcggcagg cgttttgatg cagccggagg cgacgtaaaa
     5761 atgatcgcgc cccctgacat cgatgacacc gaaacccgtt acgcgactgc ccgggtcaat
     5821 acccaagata cggacggttg ccgacatatt cacagcaaac cgtgttgaat cagcttctta
     5881 cgcagggtat tgcggttcag tcccaacatg acggaggctt tggactggtt gccgccgcat
     5941 tgttccatca cgcacaccag cagcggtttt tccacctgat gcaataccat atcgtacacg
     6001 ccgcaaggtt cggtaccgtt caggtctttg aaatattgtt ccaaattttg tctgatacat
     6061 tgggaaatat cgggaagggt atggggcatg attgcacttt caaaggataa tcaagtgttc
     6121 agaaggcatt tgggcggtag gcgcacgccc aactgtcggt tttttcggca agtctttcaa
     6181 gataacctgc aagcatgtcg tattgcgccg ccgcactgtc caagcggttg atttcacgac
     6241 gtgtctgttc gccgtcgggc atttcgtcga tgtaccagcc tatgtgtttg cgtgcgatgc
     6301 gcacaccggc ggtgtcgccg taaaacgcgt gtatggcgcg gatgtggttc aaaatagcgg
     6361 cggcgcattc tgccaaactc aaggcaggcg gcaaaacacc gtgttcggca taatgtttca
     6421 aatcgcggaa gaaccacggc ctgccttgcg cgccgcgccc tatcataatg ccgtcggcgg
     6481 cggtttgttt gaggacggct tgggcttttt gcggcgaagt aatgtcgccg ttgacccaga
     6541 ccgggatgtt cagacggcat ttggtttcgg cgatgagttc gtaacgtgct tcgcctttgt
     6601 acatttgcgt acgcgtgcgt ccgtggacgg caagggcggc gatgccgcaa tcttcggcga
     6661 ttttggcgat aacgggcagg tttttgtggt cgtcgtgcca gcccaaacgg gttttgaggg
     6721 taacgggtac gcccgccgct ttgaccaccg cctccaaaat ggcggcaacc agcggctcgt
     6781 tctgcatcag cgcgctgccg gcttggacgt tgcacacttt cttggcggga cagcccatat
     6841 tgatgtcgat aacctgcgcc ccaaggctga cgttgtaacg cgcggcatcc gccatctgct
     6901 gcggatcgct gccggcaatc tgcacggcaa cgatgccgcc ttcatcgaca aaatcactgc
     6961 ggtgcaaggt ttttctagta tttctgagcg tcgggtcgct ggtcagcatt tcgcacaccg
     7021 cccaacccgc gccgaaatcg cggcagaggc ggcggaaggg tttgtcggta atgcccgcca
     7081 tcggtgcaag tgcgatgggg ttgtcgataa aatagccgcc gatgtgcata atgggcccgc
     7141 gtttcaaaaa agtacgccat tgtacatttt ttaagcagga tttccaatct ccggacgcgc
     7201 ccgcgattgg ggcagacacc gttttatggc ataatccgca cacagattcc ctgccccgcc
     7261 actcacagga gggcagttta tag
//
LOCUS       DAWKOG010000099         7201 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.099, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000099 DAWKOG010000000
VERSION     DAWKOG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7201
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.099"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(85..954)
                     /locus_tag="WJK66_001480"
     CDS             complement(85..954)
                     /locus_tag="WJK66_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ7579185.1"
                     /translation="MSANEYTQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
     gene            complement(1049..1966)
                     /gene="hisB"
                     /locus_tag="WJK66_001481"
     CDS             complement(1049..1966)
                     /gene="hisB"
                     /locus_tag="WJK66_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEZ7579186.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            complement(1963..3060)
                     /locus_tag="WJK66_001482"
     CDS             complement(1963..3060)
                     /locus_tag="WJK66_001482"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEZ7579187.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGSIPNLIVMRTV
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNTFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            complement(3106..4395)
                     /gene="hisD"
                     /locus_tag="WJK66_001483"
     CDS             complement(3106..4395)
                     /gene="hisD"
                     /locus_tag="WJK66_001483"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEZ7579188.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLKAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            complement(4392..5291)
                     /locus_tag="WJK66_001484"
     CDS             complement(4392..5291)
                     /locus_tag="WJK66_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEZ7579189.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            complement(5395..6048)
                     /locus_tag="WJK66_001485"
     CDS             complement(5395..6048)
                     /locus_tag="WJK66_001485"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEZ7579190.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            complement(6127..6780)
                     /locus_tag="WJK66_001486"
     CDS             complement(6127..6780)
                     /locus_tag="WJK66_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEZ7579191.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQVASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFNLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGDQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            7058..7142
                     /locus_tag="WJK66_001487"
     tRNA            7058..7142
                     /locus_tag="WJK66_001487"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7092..7094,aa:Leu,seq:caa)
BASE COUNT         1476 a         1718 c         2122 g         1885 t
ORIGIN      
        1 cataatcggg actgatttta agcgtggggt gataaaaatg ccgtctgaaa tatgttcaga
       61 cggcattgtg tttggaaaag gcaatcagtg ttctgccaat ttcaggtaaa cgccggaaac
      121 gtcctgttcg ccgtagccgg cttcgactgc tttgcggtag ctggcagcaa cggtttcgac
      181 ggcgggcagg gtgttgcctg cctgttcaag ctctttgacg gcgaggttga ggtctttgga
      241 ggcgtgtttg agggcgaagg cgggtgggaa ttcgcggttt gcccacaggg attttttggt
      301 ttggaacatg ggcgagtcca ttgccgagcc gccgatggct tcgacgatgg tgtcggtatc
      361 gatgccgaac tgccgcgcca tcagcatcgc ttcgctgtac gcttcgccga aaatgcccaa
      421 gagcgagttc aagacgagtt tcgcgcccga acctttgccg acatcgccga aatggaaggt
      481 ttttttgccg acgagggaaa atattttttg cagcgggttt aaaacggctt cgctgccgcc
      541 gaacagaatc agcagcgtgc cgttggtggc gggcccgacc gatccggaaa cgggtgcttc
      601 ggcaaactgt ccgcctgcgg cttcgacaag tgctttgacg gcgaggtttt cggtcgggga
      661 gatggtgctc atgttgacga tgattttgcc ggccaatccg tcgcggactc cgttcaggat
      721 gtcgcacacg gcggcatagt cggaaaccat caggaaaatg acgggatagt cgcggacgag
      781 ttcggcggtg ttgccgtaaa cttttgcgcc tttggcggag atgggggcag ttttgtcggg
      841 cgagcggttg tatacgccga cttcgatgcc gccgtccaag agccgcgtta ccataggcag
      901 acccatttgc cctaagccta tccagccgat ttgtgtgtat tcgtttgcgg acatgatgtt
      961 tactcctttt ctgtttgtag tgtagtgtaa tcttatctgt tccgataccg tctgaaaagc
     1021 ctgcgggtgt ttcagacggt atggtttttt atgcggtcag cgtgcctttg gtcgaggggg
     1081 tctgtcctgc catgcgcggg tcgtgttcga ctgccatacg cagggcgcgc ccgaaggctt
     1141 tgaatacggt ttccgcctga tggtgggcgt ttttgccgct gaggttgtcg atgtgcaggg
     1201 tcatcatact gtggttgacg atgccgtgga aaaattcttc aaacaaatcg acatcgaaac
     1261 gtccgattag tgcgcgggta aattcgatgt tgtacacgag tccggggcgg ccggaaaggt
     1321 cgatgacgac gcggctgagg gcttcgtcga gcgggacgta ggaatgtccg taacggcgga
     1381 tgcctttttt gtcgccgagt gcctgccgga ttgcttgtcc gagtgtgatg ccgatgtctt
     1441 cggcggtgtg gtggtcgtcg atgtgcaggt cgcctttgca gctgatgtcg atgtcaatca
     1501 tgccgtggcg ggcgatttga tcgatcatgt gttcgaggaa gggtacgccg gtatccagcc
     1561 tgcttttgcc gctgccgtcg aggttgatgg agacggtgat ttgggtttcg caggtattgc
     1621 ggttgacggt aacgctgcgg cagccggcag cagatgcggt ttcggcaatt tgggtttcgg
     1681 cagttccggt gctttctgcg gcggtttcgg ggacagtgcg ttcgcggtgt tttctgtcga
     1741 gccagccttt gggtttgccg gtgtgttttt cgagttttgc catcaggctg ggacggatgc
     1801 cgcgtgcatc tgggtcttct gcctgctttt caaggcgttg tttgagtttg tagagtgcga
     1861 cgggggtacg gtagccgcag agtttggcga gcttggacag cgaacctgct tcttgggcga
     1921 gggtcagaaa gttgtgcagt tggcgttgtg ttttagtcaa attcatagga aatccgttgg
     1981 ttggcagtaa agttggcgaa tgatgttgag aacggcatcg ttttgtgcgg ggctgcctac
     2041 ggtaatgcgc aggcagtgtt ccaaaagcgg gtgcgcgcca tgcagttttt taaccaagat
     2101 gcggttttgt ttgagcgtgt caaacaacaa atcggcatcg ggtacgcgta tggtaatgaa
     2161 gtttgcctga cttggaaagg tgttcagacg gcatattttg cccaattcgg cgaacatccg
     2221 ttcgcgttcg tttttcaggc tgtcgatgtt ggcagagatg atgccgtagt gttgcagggc
     2281 gagtttggcg gtggtcaggc tcagttggtt catattgtag ggcggcagga ttttttgcag
     2341 ttcgccgatg acttcgggac agcctgccgc atagccgata cgcagtccgg caaaaccgat
     2401 tttgctgacg gtacgcatga cgatgaggtt gggaatgctg cccgcctgcg gcaggaagct
     2461 gtcgccgttg aatgcgccgt aggcttcgtc gacgacgacg atgccgtctg aagcttcgat
     2521 gacggcttcg atttcggcac gcgtgaaaca tacgccggtg gggttgttgg ggtaggcgat
     2581 aaaggtcagg gcagggcggt gcttcctgac ggcttcgagg acggcgggca ggttgagggt
     2641 gaaatctccg ttcagtggaa cgccgacata atccatgccg tacagcgcgg cgttgtggcg
     2701 gtacatgacg aaactgggtt cggctgccaa cattgccgcg cccggtttgg cggtcagcat
     2761 cgtgatgaac tgtatcagct cgtccgagcc gttgcccagc gcgatgtcgg cgcagtcggg
     2821 aatgtcgaac gccgaacgta atgcttcctg taaaccgctg ccggagggat tggggtaaag
     2881 atggatgggc gcggcggcaa gccgtgcctg ccattcctgc aacagcgttt catgtccggc
     2941 aaaagggtgg acgggacttt ccatcgaatc gagtttggca aagccgggcg gaacgtcggc
     3001 aatctgatat gccgacatag cttgtatgtc gtcgcggatg aaggagcgga cggatttcat
     3061 cgtgtttcct taatggttgg aatatgcctg tacgccgttt cggcattatt tcatacggaa
     3121 ctctgccgcg cgggcgtggg cggtcaggct ttcgccgtgt gccagcacgc tggcggtttc
     3181 gcctaatttt tgcgcgccct gttccgaaac ctgaatcagg ctggagcgtt tttggaaatc
     3241 atatgtcccc aaaggcgagg aaaagcgggc ggttcggctg gtgggcaata catggtttgg
     3301 accggcgcag tagtcgccga ggctttcgcc ggtgtagcgt cccatgaaaa tcgcaccggc
     3361 gtggcggatt tttttcgccc attcctgcgg attttcgact gacagttcca agtgttcggg
     3421 ggaaatgtag ttggcgattt cgcaggcttc gtccaagtct ttggcgagta tcatcgcgcc
     3481 cctgttgccg agcgaggctt cgatgatgtc gcggcgcggc atagtttcga tcaggcggtc
     3541 catagcggct tctacttcgt cgagatacgc ttgcgacgtg ccgatgagga tggcttgggc
     3601 aatttcgtcg tgttcggcct ggctgaacaa atccatcgcc acccaatcgg caggtgtcgt
     3661 gccgtcggcg atgaccagga tttcagacgg ccccgccacc atgtcgatgc cgaccacgcc
     3721 gaacacgcgg cgtttggcgg cggcgacgaa ggcgttgcct ggaccggtga ttttatcgac
     3781 ttgggggatg gtttccgtgc cgtaggcgag ggcggcaacc gcctgcgcgc cgccgacggt
     3841 gaagactttg gttacgccgg cgacgtatgc ggcggcaagt acgatgtcgt tgcgttcgcc
     3901 ttttggtgtc ggcacgacca tgatgatttc tttcacacct gcgacgtggg cgggcatggc
     3961 gttcatgatg acggaactcg gatacgccgc cttgccgccg gggacgtaaa tgccgacgcg
     4021 gtcaagcggt gtgatttgtt gtcccaacag cgtgccgtct tcatcggtgt agctccacga
     4081 ttccattttt tggcgttggt ggtagctttc gacacggcgg gcggcggtct gcaatgccgt
     4141 ctgaacgtcg ttcggaatgc gctcgaacgc cgctttcaaa tcggcttgcg tgagtattaa
     4201 atcatcgatg cttttagcgt ttgtctgatc gaatttgttg gtgtattcaa tcaaagccgc
     4261 atcgccgcgc ttttgcacgt cggcgcaaat gtcggcgacg atgcgttcgg tttcggggtt
     4321 ttgcgcggtt tcaaaagcca gcagggcttt gagtccggct tggaaatcgg gcgattgggt
     4381 gttgagtttt ttcatgattt ggttttttcc ttttttattt ttggattggg ggtcgtctga
     4441 atattctctt gttcagtttc tgtctctgtt ttgggtctga tatacgcttt ttctcccccg
     4501 ctaccaaact tcaattcggg caggcatttt tgttgcagac cgagtttggc taatacctct
     4561 tcgctacgag aggtagtgcg gaaacggtaa cgggattgtt ttctaccgtt tttggtttcc
     4621 tcaaccattg tttcaaaacc gtccacttcg tatgcactga caacagcgtt ttgcagaccg
     4681 gtgtgaacgc cgataacgta tttcactttt gaagcaacat ctttaccgat aacccagttg
     4741 cccaacgtac gcgatttgag gttggcatcg tcttggttgt cgaaaaggta gtctaattct
     4801 tcgtcagtat ctaaatcgaa agcattgtgg attttgatgg cgagaatcag cccgtcgggg
     4861 ttaatctcgt taagtgggag agaagagaat ccaaagcggc gatctagttc ttctacgcta
     4921 ataccacccg gaccatgccc ggcaatttta tttttgagtt ttttacccaa gacagattta
     4981 acaaaatgaa ttaaggcaga ttcggcggcg agtgcttcga cttcagtcag atgatagctg
     5041 atgatatagc gaccgagttt cttgcatttg gagatggctt tgagtttccg atcgataatc
     5101 tcgccggaga ctggatcttg tgaacgacta gcaacccatt catgctcgaa gatacgatta
     5161 ccgcagcctt tgccgatgta gaggattttg tcttttttca ggtcggtcag acaatagaca
     5221 taaaaccgac gctcgccgtt attcaaaacg gaaagggttg aatctgagaa tttttttatt
     5281 tttgcaacca ttttaacttc tcctatacag tttactgccg gtgatatttt cagacgacct
     5341 gtcggcggga acggataggg tcgtctgaaa acgcatcttt attcaaatga atgcttactt
     5401 cgccttcact gcgccgccga acgcctgaat aatcggctcc agcagcgcgt atttcgtttt
     5461 caaagcagcc ttgttgacca ccaggcggct ggaaatgtcg acgatgtgtt cgactgcttc
     5521 caagccgttt gccttcaagg tgttgcccgt cgaaaccaag tccacaatcg catcgctcaa
     5581 gccgaccagc ggcgcaagtt ccatcgagcc gtacagtttg ataatgtcca catggacacc
     5641 cttgccggca aaatgagatg ccgcgatttc aggatacttt gtggcaatct tcagacggca
     5701 tccgggttgc gaagctgctt cgtaatcaaa ccctttacgc acagcaacca tcatgcggca
     5761 cttggcaatc tccaaatcca aaggccggta aagccccgtg ccgccgtgtt cgatcagcac
     5821 gtctttgccc gcaatgccga agtccgccgc gccgtagcgg acataagtcg gcacatcggt
     5881 tgcgcggaca atgacaaggc ggatgttttc atggttcgtc ccgataatca gcttgcgcga
     5941 tttttcaggc tcttcagtcg gaacaatgcc ggcagcggca agcagcggca gcgtctcctc
     6001 aaaaatgcgc cccttggata aggcgatggt caaagcatta tcctgcatgg tgtggatgtc
     6061 ctttgtcggt tgaaacaata atgccgtctg aacaggcgcc gacggtgcag atggcatacg
     6121 gttttatcag agcagggtcc ttacatcggc agcaatcgtt tccggctcgc ttccgtaagg
     6181 cgagaaaatg gcaacctcac cgtttttgtc gatgagatac gcaccggaag agtggtcgac
     6241 caaatagttt tcgctgtcgt ctttttgatt gactttggca gaaaccacgc ggtattgctg
     6301 cttgatgacc ggcaggtttt ggtcgcccgt tgccgtcaga ccgataaagt ccggattgaa
     6361 ctgtttggca tacttgccga tgatttcagg cgtgtcgcgt tccggatcga tgctgacgaa
     6421 caccactttc acgtccttag cctgcccgcc caactgcttc aacgtgtcgc tgtacgtcaa
     6481 aagctctgtc gggcagacat cgggacagtg cgtaaagccg aaagacagaa tcacgacctt
     6541 gcctttcaaa tcgctcaggt tgaaaggctt gccttcgccg tcggtcagcg tgaaatcgcc
     6601 gccgatgtct tccttacgca tatccgtacc gcgcgtttgc ggctttgccg cattttccgc
     6661 agccgacgcg gatgcacttg aagaagcgac ttgcgccgca ctgttgtctt gaggtttgca
     6721 ggcggcaagc gcggcaagta cgaaaacgcc cggcaaaaag gaacgcggta cggaaaacat
     6781 gacaatctcc caagaataaa tagcaaaacc tgattcgtac ggaatttgaa tatttccatc
     6841 ttaacgcgtt tggcagattt aatgtagtat ttttttacgt tgtctgcacg gcaggaattg
     6901 ccgaatcata cagaatcttt ggagcggaaa gatacagtaa gattaaaata ataaaaattc
     6961 tatttaaaat caaaaataaa ataaaaacag ataaaataat ttcaaaaaaa aggtttacag
     7021 aatttcaaaa gccattataa tgacgacttc ttcaccagcc caggtggcga aattggtaga
     7081 cgcaggggac tcaaaatccc ccgccgcaag gtgtgtcggt tcgagtccga ccctgggcac
     7141 cacaaccgct tttagagcgg ttattttttt gtctattgct gtttaatgaa atagataaga
     7201 c
//
LOCUS       DAWKOG010000100         7102 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.100, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000100 DAWKOG010000000
VERSION     DAWKOG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7102
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.100"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(69..428)
                     /gene="rnpB"
                     /locus_tag="WJK66_001488"
     ncRNA           complement(69..428)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="WJK66_001488"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(507..1610)
                     /gene="prfB"
                     /locus_tag="WJK66_001489"
     CDS             complement(507..1610)
                     /gene="prfB"
                     /locus_tag="WJK66_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEZ7579192.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIATGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(1713..2606)
                     /locus_tag="WJK66_001490"
     CDS             complement(1713..2606)
                     /locus_tag="WJK66_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEZ7579193.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRRIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PSKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIRL
                     GEEAARAALPEIKRKLAAYRY"
     gene            2948..3397
                     /locus_tag="WJK66_001491"
     CDS             2948..3397
                     /locus_tag="WJK66_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ7579194.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            3471..3779
                     /locus_tag="WJK66_001492"
     CDS             3471..3779
                     /locus_tag="WJK66_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579195.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            3895..4737
                     /locus_tag="WJK66_001493"
     CDS             3895..4737
                     /locus_tag="WJK66_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEZ7579196.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLKNVDADRVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            4727..5473
                     /locus_tag="WJK66_001494"
     CDS             4727..5473
                     /locus_tag="WJK66_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HEZ7579197.1"
                     /translation="MQPEISAQYQHRFAQAIREGEAADGLPQERLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLLDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLSA"
     gene            5495..6082
                     /locus_tag="WJK66_001495"
     CDS             5495..6082
                     /locus_tag="WJK66_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEZ7579198.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTALDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            6088..6267
                     /locus_tag="WJK66_001496"
     CDS             6088..6267
                     /locus_tag="WJK66_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEZ7579199.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPYCREYKRQL
                     QTIKRSLAKTTRTSK"
     gene            6508..6948
                     /locus_tag="WJK66_001497"
     CDS             6508..6948
                     /locus_tag="WJK66_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579200.1"
                     /translation="MIKETLMRPIFLSFVLFPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
BASE COUNT         1794 a         1891 c         1629 g         1788 t
ORIGIN      
        1 acacaaccta aataaaaatg ccgtctgaac catatttcag gtttcagacg gcatttgcct
       61 gtcggatgca caccggacag gcggtaagcc gggttctgtc tcggacagtc attcctctag
      121 gcataccgtt gccggtatgc tcaagcaacc tacccgaacg ctcggcgggc agcgtcattg
      181 cgttctgttt ggtcttgctc cgaatggggt ttggcctgcc gcatattgtt accaaatgcg
      241 cggtgcgccc ttaccgcacc ttttcaccct tgcctgtgct gccaaagcag ccatcggcgg
      301 ttttgctttc tgttccactt tccgtcgcgt taccgcgccc ggccgttaac cggcattcta
      361 ccctgcggag cccggacttt cctccccgta tgccttacgc gatacgcggc gactgtctgc
      421 ccgtcccgtg tgcggcgcgg attataacac gaaacgcaaa aatgccgtct gaaacggtac
      481 aggtttcaga cggcatacag cctaaactac acgccctgtt tcaggctggc ttcgataaag
      541 ccgtccaagt cgccgtccaa tacggctttg gtgttgccga cttcgtagcc tgtgcgcaag
      601 tctttaatgc gtgaggagtc caagacgtac gaacggattt ggctgcccca acctacatcg
      661 gatttacctt cttccaacgc ctgtttctct tcgttgcgtt tgcgcatttc caattcatac
      721 agtttggact tcaacatttc catcgcggcg gctttgttgg cgtgttgcga acggtcgttt
      781 tgacattgca ccacaatccc cgtcggctcg tgggtaatac gcacggcgga gtcggttttg
      841 ttgatgtgct gaccgcccgc gcccgatgcg cgataggtgt cgatacgcaa atcggcgggg
      901 ttgatttcga tttcaatgga atcatcgatt tcagggtaaa cgaacacgga ggcaaacgaa
      961 gtatggcgtt tgttgttcga gtcaaacggt gaatagcgca ccaagcggtg aacgccggtt
     1021 tcggtacgca gcaaaccata ggcgtattcg ccttccactc ggatagtcgc gcggttgatg
     1081 cccgcgattt cgccgtcgtc ttcttcgagg atttcgattt tgaagccttt gcgctcggcg
     1141 tagcggctgt acatacggaa cagcataccc gcccagtctt ccgcttccgt accgcccgcg
     1201 cctgcggtga tgtcgataaa gcagttgttc gggtcggcgg gctggttgaa catccgtttg
     1261 aactccaaat ccgccatctg tttttccagc cccgccacat cttcctgcac ggcggcaaaa
     1321 ccttcttcgt cgttttcttc gacggtcatt tcaatcagca tgcggttgtc ttcgatgccc
     1381 gtcgcgatgt tgtcgagcgt caacacgatg ccttcgagga ttttgcgctc tttgccgatt
     1441 tcttgggcgc gtttcgggtc gttccaaagt tcggggtctt cggaaagacc gataacttct
     1501 tccaatcggt ctttcttacc ctgataatcc atataaactc ggatgtcttc gctgcgcttt
     1561 tccaaatcat tcagggtatt gttgagctgg ttgattactt cggcttccat gattcttttg
     1621 ttctttcaaa attttagggg cgtattgtac gggattcggg tatttttttc tatggataaa
     1681 gccttctgga aacacgttcg gacggcatag cgtcaataac ggtatgccgc cagtttgcgt
     1741 ttgatttcag gcaatgcggc acgtgctgcc tcctcaccca accggatggc gcgttttttc
     1801 tgatcgaatc cgccgactgc acccaaatcc aaaacctgcg gtttgataac cacatccgcc
     1861 tgccccaact cattttgcaa cgcggaaacg ctcattacgt tcagcgtctg atcgagataa
     1921 gagaagaagc cttggctgat gtttttgctc ggacgggcgg aaatatcgac ggcaatcacg
     1981 aaattcgccc cctgccgccg ggcggcactg acgggcacgg gctgcgacag accgccgtca
     2041 acatatgtat gcctgccgat gataacgggt tggaacacat tgggaatggc ggcggaagcg
     2101 cgcacagcct gcccggcatt cccttgattg aaagcgacgg ccttgccggt ttcaaaatca
     2161 gtagcaacgg cggcaaattt gatgggaaac tgctgaatcc gcctgccgcc gacttttcgg
     2221 ttgatgtaat tttgcagctt ttcgcctttg ataaaaccac tggtggacaa ggttaaatcg
     2281 accaaatcgg ttttacctaa aatttcggct tccaattcga ggcggtcggg cgacataccc
     2341 gatgcaaaaa ggctgccgac tatcgaacct gccgatgtgc cggtaaccac cttcacagga
     2401 ataccgtttt ctttcaaaac cttaataata cctacatggg caaatccttt agatgcgccg
     2461 ccaccgagtg ccaaaccgac cactgcggcg ggtttggcgg tttgcaccgg cttgcgggca
     2521 gcattatttc ccgccgtgcc gcaggcggca agcaacgcgg cggcggcgat tgccaaaagc
     2581 ggtctgattt ttgaaaacgt taccatattt tccattcctt tatatatcgc accccgtcaa
     2641 aaagagggat tgcttttctt aacaaccccc tttgacagcc aagcaaatgg gggctttgtt
     2701 aagtcatcat caaaattaat atttcttttt ttcctttacg gaaattatat ttgaaggcat
     2761 actatccaag gtgggaatta tctcacaaca ccgccgttat ccaaatatcc cgcctttttc
     2821 ccttcctttc catcaaaata ctttcttttt atattcatta acttgttaaa tcattggctg
     2881 ccgggtgtca gtttttccga caaaatccgt ctaatggggt atcaacagaa ccaaaacagg
     2941 aacacttatg aaaatcggaa caacttggca gacggcatcc gctatgctgg ttttgcgtct
     3001 gtttgccgca tatgaatttt tggaatcggg tttgcaaaaa tggaacgggg agaattggtt
     3061 ttccgaaatc aacgatcagt ttccattccc gttcaacttg ctgccggacg cgttaaactg
     3121 gaatctcgcc atgtatgcgg agcttttact gcccgtactg ttgcttttgg gtttggcaac
     3181 gcgtctgtcg gcattagggc tgatggtcgt taccgccgtc gcttgggctg cggttcacgc
     3241 cggttcgggt tacaatgtct gcgacaacgg ttataaaatg gctttaattt atatcgtggt
     3301 attaatcccg ctgcttttcc agggtgcggg cggatggtcg ctggatacgc tgctgaaaaa
     3361 acggttttgc ccccaatgcc gtctgaagca agattgattc agtcgtggaa tctgacttta
     3421 aacattccaa ccttatctcg ttaacttgat attctgaaaa ggaaatagaa atgaacaaaa
     3481 acattgctgc cgcactcgcc ggtgctttat ccctgtcttt ggccgccggt gcagttgctg
     3541 ccaacaaacc agcaagcaac gcaacaggcg ttcataaatc cgcccatggc tcttgcggcg
     3601 cgtccaaatc tgccgaaggt tcgtgcggcg cggctggttc taaagcaggc gaaggcaaat
     3661 gcggcgaggg caaatgcggt gcgaccgtaa aaaaaaccca caaacacacc aaagcatcta
     3721 aagccaaggc caaatctgcc gaaggcaaat gcggcgaagg caaatgcggt tctaaataat
     3781 cccacccctt caaaccaagc cgcgtttttc agtaaaatgc ggctttttta acggcaaaca
     3841 aagatttttt aacaagcaca tcattctttt gtgccatccg aaccgggtaa aaatatgatt
     3901 caacacgcag gcttgggcta ccgccgcgac ttggcggaag actttctctc gctttccgaa
     3961 aacagcccga tatgctttat cgaagccgca ccggaaaact ggctgaaaat gggcggctgg
     4021 gcacgcaaac agtttgaccg tgtggcggaa cggctgccgc tggcgttgca cggattgtct
     4081 atgtcgctgg gcgggcaagc accgctggat actgatttga tagacggcat caaagaaatg
     4141 atgcgccgtt acgattgcac gtttttctcc gaccatttga gctactgcca cgacggcggt
     4201 catctttacg atttgttgcc gctgcccttt accgaggaaa tggtgcatca tacggcgcgg
     4261 cgtatccgcg aagtgcaaga ccgtttgggc tgccgcatcg ccgtggaaaa cacgtcctac
     4321 tatctgcatt ccccgcttgc cgagatgaac gaggtcgagt tcctcaacgc ggttgcacgt
     4381 gaggccgatt gcggcattca tctggacgtg aacaatatct acgtcaacgc cgtcaatcac
     4441 ggcctgttgt cgccggaggc ttttttgaaa aacgttgacg cagatcgcgt gtgctatatc
     4501 catattgccg gacatgacgt ggaaacgccg gaattgttga ttgatacaca tggcgcggca
     4561 gttttgccga ctgtttggga cttgctcgaa cttgcctatg ccaagctgcc gacgattccg
     4621 cccaccctat tggaacgcga ttttaatttc ccgccttttg ccgaactcga agccgaggtc
     4681 gccaaaattg ccgactatca aacgcgtgcc ggaaaggaat accgccgtgc agcctgaaat
     4741 ctccgcccaa taccaacacc gtttcgccca agccatacgc gagggtgaag ccgccgacgg
     4801 actgccgcaa gagcgcctga acgtctatat ccgcctgata cgcaacaata tccacagctt
     4861 tatcgaccgc tgctataccg aaacgcggca atactttgac agcgaagaat ggagccgtct
     4921 gaaagaaggt ttcgtccgcg acgcgcacgc ccaaacgccc tattttcaag aaatccccgg
     4981 cgagttccta caatattgcc aaagcctgcc gcttttagac ggcattttgg cactgatgga
     5041 ttttgaatat acccaattgc tggcagaagt tgctcaaatt ccggatattc ccgacattca
     5101 ttattcaaat gacagcaaat acacaccctc ccctgcggcc tttatccggc aatatcgata
     5161 tgatgttacc gatgatttgc atgaagcgga aacagccttg ttaatatggc gaaacgccga
     5221 agatgatgtg atgtaccaaa cattggacgg cttcgatatg atgctgctgg agataatggg
     5281 ttcctccgcg ctttcgtttg acaccctcgc ccaaaccctt gtcgaattta tgcctgagga
     5341 cgataattgg aaaaatattt tgcttgggaa atggtcaggc tggactgaac aaaggattat
     5401 catcccctcc ttgtccgcca tatccgaaaa tatggaaggc aattccccgg gccaaaacca
     5461 tctatccgca taaaattacc ttgttcccga tactatgccg ctacccgacc tgaccgatgc
     5521 cgaattaata gagtcgcgta aactgcttct gcattttgcg cggcttcagt tgcccgacca
     5581 ccctgatttg gctgaagatt tagtgcagga aacattgctg tccgcataca gcgcaggcga
     5641 cagttttcaa ggcagggcac ttgtcaacag ctggcttttt gccatattga aaaacaaaat
     5701 tattgacgca ttacgtcaaa tcggaaggca gaggaaagtc tttaccgcac tggatgacga
     5761 gctactggat gaagcatttg aaagccattt ttcccaaaac gggcattgga cgccggaagg
     5821 gcagccgcaa cattggaaca ctccggaaaa atcattaaac aacaacgaat tccaaaaaat
     5881 tctgcaaagc tgcctataca acctgcctga aaacaccgca cgggtattta ccctgaagga
     5941 aatactcggt ttttcatccg acgaaataca acaaatgtgc ggtatcagca cgtccaacta
     6001 ccacaccatt atgcaccgcg cccgagaatc attgcgccaa tgcctgcaaa tcaaatggtt
     6061 caaccaagaa aacccgaagt aaacgttatg aaaaaatgcc gcgatatcgc cctgcttctt
     6121 tccaaacatc aggaccggga aaccaccccg ggcgagaaga tttccatata cacacacctg
     6181 ctgttctgtc cgtattgccg tgaatataaa agacaacttc aaaccatcaa aagatcactg
     6241 gcaaaaacaa ccagaacttc aaaataaatg ccgtctgaaa aggcttcaga cggcataagc
     6301 tgacggaaac aaatcaaacc gatttactgt tatcggcagt tcatccataa tacacgcttc
     6361 aaaagcagca tattcctcca tacggaatat ataaatacgc aaaacacgaa ggctgcatca
     6421 atttgctata tttgctttat ttaccttatt tcacagacgg cgctacccct cccgcccaac
     6481 ccgttctttc tgaatgagca gatttcaatg attaaggaaa ccctaatgcg cccaatcttc
     6541 ctatctttcg ttttattccc tattttgata accgcctgca gcacaccgga caagtctgcc
     6601 cgatgggaaa atatcggcac aatctcaaac ggcaatattc atacatatat caataaagac
     6661 agcgtgagaa aaaacggaaa tctgatgatt ttccaagata aaaaagttgt taccaatcta
     6721 aaacaagaac gttttgccaa cacccccgca tacaagactg ccattgccga gtgggaaatc
     6781 cactgcaaca acaaaacata ccgcttaagt tcgctacaat tgtttgatac aaaaaacacg
     6841 gaaatttcca cacaaaacta cacagcctct tccctccgcc cgatgagcat cctgtccggg
     6901 acattaaccg aaaaacaata tgaaaccgta tgcggaaaaa aactctgatt gcaacttata
     6961 cacaaactta cccacaaacc ttatcataaa aatgccgtct gaaatactga aatatcagca
     7021 tttcggacgg cattttgcca tttctccgaa aagttatcct cgaagttatc cacattattt
     7081 ttttaaaccg gcttccatcc ga
//
LOCUS       DAWKOG010000101         7079 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.101, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000101 DAWKOG010000000
VERSION     DAWKOG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7079
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.101"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            126..1976
                     /locus_tag="WJK66_001498"
     CDS             126..1976
                     /locus_tag="WJK66_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ7579201.1"
                     /translation="MYLPSMKHSLPLLATLVLAACSSTNTLPAGKTPADNIETADLSA
                     SVPTRPAEPEGKTLADYGGYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKS
                     LGARKQWTLFAQEYAKLEPAGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
                     EQAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIG
                     KEARKSPNAAALLSEMESGLSLEQRSFAWGVLGHYQSQNLNVPAALDYYGKVADRRQL
                     TDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKL
                     YKQAAATGRNFYAVLAGEELGRKIDTRNNVPDADANSVLRMAEDGAIKRALVLFQNSR
                     TAGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSRVGAQGLMQVMPATAREI
                     AGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMANAAYYASLFGAPHIPLKQRMGIVPAR"
     gene            2265..2477
                     /locus_tag="WJK66_001499"
     CDS             2265..2477
                     /locus_tag="WJK66_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEZ7579202.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            2609..3055
                     /locus_tag="WJK66_001500"
     CDS             2609..3055
                     /locus_tag="WJK66_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEZ7579203.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTEAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTA
                     VDAAVAETGAAGMADMGKVMGVLKTRLAGKADMGEVNKILKAVLTA"
     gene            complement(3133..3375)
                     /locus_tag="WJK66_001501"
     CDS             complement(3133..3375)
                     /locus_tag="WJK66_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579204.1"
                     /translation="MPSERNRVSDGILSDGAQTESVYRQNPFRCIETVLPLPIRYWDV
                     IASALECNYGAANQAIERQRQRAAAVAVHERVAKFL"
     gene            complement(3380..3772)
                     /locus_tag="WJK66_001502"
     CDS             complement(3380..3772)
                     /locus_tag="WJK66_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEZ7579205.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     AYRPDTPPENTSAETAPRPAKKGLKLVK"
     gene            complement(3844..4449)
                     /locus_tag="WJK66_001503"
     CDS             complement(3844..4449)
                     /locus_tag="WJK66_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEZ7579206.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            complement(4551..6227)
                     /locus_tag="WJK66_001504"
     CDS             complement(4551..6227)
                     /locus_tag="WJK66_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEZ7579207.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPAE
                     PAVYTAVEALAGSPTPLVAHLFGHADFGIPPVYLWVAAAFKHLLSPWAADPYDAARFA
                     GVFFAVVGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPTVHFLNPAAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDDHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGVVWMLAVLVLLAVNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIDIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVRLPQNADASQGWQTVWQGARPRNKDSKFALIRKIGENILKTTD"
     gene            complement(6373..7002)
                     /locus_tag="WJK66_001505"
     CDS             complement(6373..7002)
                     /locus_tag="WJK66_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEZ7579208.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGIKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNSANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGVYILIENNSFDMLWTVSGQEKIL"
BASE COUNT         1835 a         1949 c         1892 g         1403 t
ORIGIN      
        1 attatgtcgg aaacattcca aaggcggtgc agtttcggca tataattcgg gcaaacgcct
       61 gttcagacgg cattttgtct tttccaaccc tgaccgttca gggttccgat tcttaaggaa
      121 atccgatgta cctaccctct atgaagcatt ccctgccgct gctggcgacc ctagtgcttg
      181 ccgcgtgttc ttcgacaaac acactgccag ccggcaagac cccggcagac aatatagaaa
      241 ctgccgacct ttcggcaagc gttcccaccc gccctgccga accggaagga aaaacgctgg
      301 cagattacgg cggctacccg tccgcactgg atgcagtgaa acagaaaaac gatgccgccg
      361 tcgccgccta tttggaaaac gccggcgaca gtgcgatggc ggaaaatgtc cgcaacgagt
      421 ggctgaagtc tttgggcgca cgcaaacagt ggacgctgtt tgcacaggaa tatgccaaac
      481 tcgaaccggc agggcgcgcc caagaagtcg aatgctacgc cgattcgagc cgcaacgact
      541 atacgcgtgc cgccgaactg gtcaaaaata cgggcaaact gccttcgggc tgcaccaaac
      601 tgttggaaca ggcagccgca tccggcttgt tggacggcaa cgacgcctgg aggcgcgtgc
      661 gcggactgct ggccggccgc caaaccacag acgcacgcaa ccttgccgcc gcattgggca
      721 gcccgtttga cggcggtaca caaggttcgc gcgaatatgc cctgttgaac gtcatcggca
      781 aagaagcacg caaatcgccg aatgccgccg ccctgctgtc cgaaatggaa agcggtttaa
      841 gcctcgaaca acgcagtttc gcgtggggcg tattggggca ttatcagtcg caaaacctca
      901 atgtgcctgc cgccttggac tattacggca aggttgccga ccgccgccaa ctgaccgacg
      961 accaaatcga gtggtacgcc cgcgccgcct tgcgcgcccg acgttgggac gagctggcct
     1021 ccgttatctc gcatatgccc gaaaaactgc aaaaaagccc gacctggctc tactggctgg
     1081 cacgcagccg cgccgcaacg ggcaacacgc aagaggcgga aaaactttac aaacaggcgg
     1141 cagcgacggg caggaatttt tatgcggtgc tggcagggga agagttgggg cgcaaaatcg
     1201 atacgcgcaa caatgttccc gatgccgacg caaacagcgt cctccgtatg gcggaagacg
     1261 gcgcgattaa gcgtgcgctg gtactgttcc aaaacagccg gaccgccggc gatgcgaaaa
     1321 tgcgccgtca ggctcaggcg gaatggcgtt ttgccacacg cgggttcgac gaagacaagc
     1381 tgctgaccgc cgcacaaacc gcgttcgacc acggttttta cgatatggcg gtcaacagcg
     1441 cggaacgcac cgaccgcaaa ctcaactaca ccttgcgcta tatttcgccg tttaaagaca
     1501 cggtaatccg ccacgcgcaa aatgttaatg tcgatccggc ttgggtttac ggcctgattc
     1561 gtcaggaaag ccgcttcgtt ataggcgcgc aatcccgcgt aggcgcgcag gggctgatgc
     1621 aggttatgcc tgccaccgcg cgcgaaatcg ccggcaaaat cggtatggat gccgcacaac
     1681 tttacaccgc cgacggcaat atccgtatgg ggacgtggta tatggcggac accaaacgcc
     1741 gcctgcaaaa caacgaagtc ctcgccaccg caggctataa cgccggtccc ggcagggcgc
     1801 gccgatggca ggcggacacg cccctcgaag gcgcggtata tgccgaaacc atcccgtttt
     1861 ccgaaacgcg cgactatgtc aaaaaagtga tggccaatgc cgcctactac gcctccctct
     1921 tcggcgcgcc gcacatcccg ctcaaacagc gtatgggcat tgtccccgcc cgctgacgta
     1981 ccgatgccgt ctgaaacccg cccggtcttt cagacggcat ttttatcccg aacggcattg
     2041 acggcgaacc ataaatataa gacaatccga aaattgtttt tcctgctttt tcaagcagct
     2101 tgacacggca caagccgacc cgttaggagg tgatgtttcc gtcacggcgc gtatcccgcc
     2161 gccgcaaggc acagcgatac ggtaaacctt caacaccgtc tgccctaccc tttccaccga
     2221 tatgatgggc agatgaaaca accgaattta ttaaaggaaa taaaatgcct gcaatccgcg
     2281 taaaagagaa tgaaccattt gaagtcgcta tgcgccgttt caaacgcgcc gtagaaaaaa
     2341 ccggcctgct gaccgagctg cgcgcccgcg aagcctacga aaaaccgact accgaacgca
     2401 aacgcaaaaa agcggcagcc gtaaaacgcc tgcaaaaacg cctgcgcagc caacaactgc
     2461 cgcccaaaat gtactaaacg ttcaagtaca gattacaggt cagccctgtg atatgaggac
     2521 acaccgcaag acctgctctg cggtgtgttt tgcttttcag acggcatcga aacccgccgt
     2581 ttccatccga catcccagcg aggacatcat gagcctgaaa atccgcctta ccgaagacat
     2641 gaaaaccgcg atgcgcgcca aagaccaagt ttccctcggc accatccgcc tcatcaacgc
     2701 cgccgtcaaa cagtttgaag tggacgaacg caccgaagcc gacgatgcca aaatcaccgc
     2761 catcctgacc aaaatggtca aacagcgaaa agacagcgcg aaaatctaca ctgaagccgg
     2821 ccgtcaggat ttggcagaca aagaaaacgc cgaaatcgag gtactgcacc gctaccttcc
     2881 ccaaatgctc tccgccgggg aaatccgtac tgcggtcgat gccgccgttg ccgaaaccgg
     2941 cgcggcaggt atggcggata tgggcaaagt gatgggcgta ttgaaaaccc gcctcgccgg
     3001 caaagccgat atgggcgaag tcaacaaaat cctgaaagcc gtgctgaccg cctgattgcc
     3061 cgaatatcgg acaaaatgcc gtctgaagcc cgtatcgcag gttcagacgg cattttcatg
     3121 gtttgatgat atttataaaa attttgcaac acgctcatgc acagcaactg ccgctgctcg
     3181 ttggcgttgc cgctctatcg cttggttagc tgcaccataa ttgcactcta atgctgatgc
     3241 aataacgtcc caatatcgaa tcggcagggg caacacggtt tcgatacacc gaaacgggtt
     3301 ttgccgataa acggattccg tttgcgcccc atcggacaaa atgccgtctg aaacacgatt
     3361 ccgttcagac ggcatagatt tatttgacca atttcaagcc ttttttggcg ggccggggcg
     3421 cggtttcggc agaggtgttt tcaggcggcg tgtcggggcg atacgcttcc aattcaaacc
     3481 ccataccttc gccggtctcc cgtgcgaaaa ggctgaggac gtgtccgaca ggtatccata
     3541 tgtcgtgcgc ctgtccgccg aagcgggcgg aaaagctgat ccaatcgttg tcgatttgaa
     3601 ggttttgcgt ggcggtcgcg ccgatgttga gcataatttc gttgtcgcgg acgtactgca
     3661 tggggacacg cgtgtgttcg ttgacccaga caagaatgtg cggcgtgagg ctgttatcgc
     3721 tgcaccattc gcaaagtgcg cggaggatgt agggtttggt ggaagtgggc ataatgggtt
     3781 ccgtgtggtg tgcctgaatg tgaaaatgcc tacaaaacgg cgggttttgc aagcattccg
     3841 ggcttatttg cgcatggctt tttcggcggg tgtcagtgct tcgataaagg cttcgcgctg
     3901 gaagatgcgc tcggcgtatt tgagcagcgg cgcggcactt ttgcccagtt tgacatcgta
     3961 gtggtcgagc cgccacagca gcggagcaag ggcgacatca atcatagaaa aatcttcgcc
     4021 gaggatgtat ttgcttttgc tgaacgaagg ggcaagcatg gtcagaccgt tgccgatggc
     4081 ttcgcgcgct tttgcctgtt ccttgttggt ggcggcgggg ttttctaaca cttggacgtg
     4141 gttgaacaat tctttttcca tacggtacag caccagccgg ccccggccgc gcataacggg
     4201 atcgccgggc ataagctgcg gatgagggaa gcgttcgtcg atgtattcgt tgatgatgtt
     4261 ggactcgtgc agcaccaaat cgcgctcgac cagcacggga acttggttat acggattcat
     4321 gacggcgagg tcttcgggtt tgttgtaaat atcgacgtct ttgatttcaa aatccatacc
     4381 tttttcgtac aaaacgaagc ggcagcggtg gctgaagggg caggtaatgc cggaatagag
     4441 ggtcatcata ataattgtcg ctcctgtgtg atgcctgcaa aacggctgat ttataaaggt
     4501 ttaatcgcgc aattatacgc gatttccggc acttaatcca gaaattcggc tcaatctgtt
     4561 gtttttaata tattttcccc gattttccgt atcagtgcga acttactgtc tttgttgcgc
     4621 gggcgcgcac cctgccagac cgtctgccag ccttgcgacg catccgcatt ttggggcaag
     4681 cggacgatac ggtagcggca ttgtacatcg ccgacgcggt gcggcaatgt gccgtactgc
     4741 gtccaaacaa tccgcgtgtg caggtcgccg ccgcctatgt cgatacactc gatgccgtct
     4801 gaaagctccc gttttaattc cggggaaagc gatgcctcca tactccggac gaccggcgcg
     4861 tggcttttcg ccgcgtccag ccacggcagg aacagcgtca tcagcaaagc ccaggtcagg
     4921 gtaacgcctg ccgcccagtt ggtaaccgcc tgcctgccgc gtatgttttt ccgggtaatc
     4981 gcccacagcc acaagggtgt gaacagtacg gcaaccgcca tcggaatggg atcgatatca
     5041 ggaacataat acgggctgaa ataggcggcg cgttcggcaa gcttggcggg ccagccgtaa
     5101 ttcatggcga aaaagcccgt ccacaggaac acggcaaaca gtccgaacgc cataatgccg
     5161 aaccagttga caaacgccgc cgcgccgcgc ctcaggctgt ccagttgcgc cgcgccgaac
     5221 agggcaagcg gcggaagcag ccagacgagg ttatcctgaa aacgctgcgg attgacggca
     5281 agcagcacca aaacggcaag catccagacg acgcccaaaa tcccccagtc ggtcgaaaac
     5341 aggcgcgtgc ggcaaaccgt ccaaaccgcc agcggcagcg cgggcaatgc aaaccaaagc
     5401 aggtttttca gatagtaaaa caaactgaat gccgtctgaa cgtgccgcac gccgccgaac
     5461 gtaccgaaaa cgtgatcgtc gagccattgc gcgaacagcg cgggctgcgt ttttgccaag
     5521 agcagcgggt aaacggtcat aagcggcagg gcaaaggcaa gtgaggcgac tgccgtcaac
     5581 atcaaacgcc tgctttgcca cggacggaaa aacatcagta cgggcaaggg cagcatcagg
     5641 gcaaatgctg ccggataagc tgctgccaac gacatcagcg tccagcccgt accgagcaga
     5701 aaagaggcgg caatcacgcg ccggcgagcc aaagaataac cgtgcagcac cagtccggcg
     5761 gcggcaaagg cggcggcagc ggggttgaga aagtgtacgg tcggaatcag cccgatacag
     5821 ccgatgagaa tcaggacgac gctgcgcccg tggtgtctgc ccaaaaagtt gaagccagca
     5881 aagccgcagg aagtcagccc gacaacggcg aaaaacacgc ctgcaaagcg tgcggcatca
     5941 tacgggtcgg ctgcccacgg cgacagcaaa tgtttgaacg cggcggcaac ccaaagatac
     6001 acgggtggta tgccgaaatc ggcatgcccg aacagatggg caaccaaggg ggtggggctg
     6061 cctgccagtg cttcgacggc ggtatagacg gcaggttcgg caggattcca caaatcgtgg
     6121 gaaaacacgc cgggccacaa ccaggcaaac gccatcaaca gcagcagcca cggcttttcg
     6181 tgggttttgg cgggcgggcg ggcatcgggc ggagtgtagg tcagcataag cgtgagaaaa
     6241 aatggacgga ttgccaagtg tagcaaatat tcgcacaaag gtcgtgcaga gactgcttca
     6301 gacggcatca gacacaaaaa gaccggcaac aaaaaagact gcacatggca gtctttgcag
     6361 atactatctt tttcataata ttttttcctg gcccgacaca gtccatagca tatcaaaact
     6421 gttgttttca atcaggatat atacccccaa tcctaaataa acaacagcaa caaaccatct
     6481 gctatatttt tccaaagttt ctccgacaga agggacttgt gccaattttt gggcagaaaa
     6541 aaccaagaga taaatcatga ctagaaaggt aagtaaagcc actatcaaat tcgctgaatt
     6601 taaggtagta aaatatggga caaagacacc aatattgtca gcaccacaac ttgcaaaagt
     6661 aatcatagcg actagaaaaa tcaggttttt attatctttg cgcaaaccct ctttggcaat
     6721 agcctctcca tcagaatctc ctaacagcaa aactttgatg cctaggaaaa ttggaatcaa
     6781 gccgagcaaa cctaaaatct ctttactagg aatataatct aagacaaatg caaaaagcaa
     6841 acttagcaat atcagactaa cagagcctag aaattgtcct aaatagatgt taatgatgtc
     6901 ttttctgctt tttcttttgg caaaaaataa cattaggata ataagtaagt ctacggctgt
     6961 cccagaatac aggattattg aagtaacgac attttgaatc ataaaacatc tcattcaaat
     7021 atatttttaa atgtattcaa acattaaacc ttgtagatgt caacttcaac cccgtcaaa
//
LOCUS       DAWKOG010000102         7057 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.102, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000102 DAWKOG010000000
VERSION     DAWKOG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7057
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.102"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            11..313
                     /locus_tag="WJK66_001506"
     CDS             11..313
                     /locus_tag="WJK66_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579209.1"
                     /translation="MTWVGGSGNAQQSSYYCEICESAGLNDTGNPDKSLGLPNNTFSN
                     PPGWGATIGALAGSRIGMPEFGTFASHAIENFDWSWYRRYREIAETIEREYSGGLP"
     gene            593..751
                     /locus_tag="WJK66_001507"
                     /pseudo
     CDS             593..751
                     /locus_tag="WJK66_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690588.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            896..2627
                     /gene="hrpB"
                     /locus_tag="WJK66_001508"
                     /pseudo
     CDS             896..2627
                     /gene="hrpB"
                     /locus_tag="WJK66_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
     gene            2720..>7057
                     /locus_tag="WJK66_001509"
     CDS             2720..>7057
                     /locus_tag="WJK66_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HEZ7579210.1"
                     /translation="MNKGLHRTVFSKKHNTMVAVAETANSQGKGKQAGTSAPASPQIS
                     DGLYSKLKTTLKTLVCSLVSVSMVLPAHAQIITDKTAPKNQQAVILKTNTGAPLVNIQ
                     TPNARGLSHNRYTQFDVDAKGAVLNNDRNNNPFLAKGSAQLILNEVRGAASKLNGIVT
                     VGGQKADVIIANPNGITVNGGGFKNVGRGILTTGAPQIGKDGALTGFDVRQGTLTVGA
                     AGWNDKGGADYTEILARAVALQGKLQGKNLAVSTGPQKVDYASGEISAGTAAGTKPTI
                     ALDTAALGGMYADSITLIANEKGVGVKNAGTLEAAKQLIVTSSGRIENSGRIATTADG
                     TEASPTYLSIETTEKGAAGAFISNGGRIESKGLLVIDTGEDIGLRNGAVVQNNGNRPA
                     ATVLNAGRNLVIESKANVNNAKGSTNLSAGGRTTINDATIQAGSSVYSSTKGDTELGE
                     NTRIIAENVTVLSNGSIGSAAVIEAKDTAHIEAGKPLSLETSNVASNIRLNNGSIKGG
                     KQVVLMADDDIQAKASHLNASGNLYIHAGKDLDLNADKDLSTQSISLRADNTALISSN
                     GNTLTAEKNLDIQAGSLSVRQSNLQSSGGNVQMSATKGNISLNQSWINASQNIDTAAL
                     QGNIISDGLTAVAEVGRVSLLANGNVDFNGLNTLIAEGDINAGSVGKGRLKMDNTDIY
                     ASAGDVKLVAGGQLDLGNGTVNGGHISLDSNKGSMVVQDVHLNARASLKVDADQTLTI
                     NNSKLNSGHNTQINTNHGHMTLNQLDAHSRRHMSISAQGKGKGKDSGQILQNDQQNSK
                     STLAADGVLSLNSSALQVLDNTTLRGGAINIKAGGGIIKRGHIDWETQDTATMRSAEL
                     KPLSGMMSIESGGNNPLTVEPGNRIVSAGDLAVNHNGTFQISARAGNNGNPSAQTASV
                     SAKGNIGIVAGEVDIDAANIAAGKDLALVATKGNISLNSIRNTFSNYQLKTDKHNITQ
                     QLTDVEQEFSKLTSDPKYRKAQDLSQMLRRKYKRRDKVFGDSEARLRGLRAKINAADE
                     AWAERQSPVKALLERKQLLQQALLTVSQPGSGHENQGSTLSGQNIKLLAAGGIRIQGS
                     KVAATQQANIQAAGFLPAPAAEELQEGRLQSAIDISGVFDTFEYGQQGSDKYGYAIFS
                     RPSEISGKTGVTLSAPNANENSRISLSAANIEAENGKIKIQSYGDQYYYARQSELYTF
                     ERRSYKTGKWYNRKHITEVKEHKNAKPDAVTLSASQGIDIKSGGNIDAYATAFDAPKG
                     SINIEAGRKLTLYAVEELNYDKLDSQKRRRFLGISYSKAHDTTTQVMKTALPSRVVAE
                     SANLQSGWDTKLQGTQFETTLGGATIRAGVGEQARADAKIILEGIKSSIHTETVSSSK
                     STLWQKQAGRGSNIETLQLPSFTGPVAPVLSAPGGYIVDIPKGNLKTQIETLTKQPEY
                     AYLKQLQVAK"
BASE COUNT         2092 a         1652 c         1677 g         1636 t
ORIGIN      
        1 tgttcatgaa atgacttggg tcggaggctc aggtaatgcg caacaaagct catattattg
       61 cgaaatttgc gaatctgcag ggcttaacga tacgggaaat cctgataaat ctttaggatt
      121 gccaaacaat acgttcagta atccgcctgg ttggggagct acaatcggag ctttagcagg
      181 tagccgcata gggatgcctg aatttggtac gtttgcgagc catgccattg aaaatttcga
      241 ctggtcatgg tatcgacgtt atagggaaat tgccgaaacg attgaacgag aatattcagg
      301 cggtttgcct taatagttga ggaggtcatg atgtttgcca aacattatca attcatcgca
      361 ctcggcatca tgctgcttct ttatatgttg attctctata cgaccgattt ttccaatctg
      421 acgtattgga tgctgttttt tatctgtttt attacaggaa aaatattagc tcgtttgtta
      481 gagaaaagct ttaaataaaa tagcagctag tcgcaaaagg tcgtctgaaa ccttttcagg
      541 cggcctttct aaaatacatc caacttccta atccctattt ttcaaaaagg aaatctatgc
      601 cccatctgca aaacctgtct ttgggcttaa aaaaaagctg cctgttatcc tgcaaacaga
      661 aatatcagaa tgcggcttgg catgtctggc ggctgtggcg ggatttcatg gtttccatac
      721 ggatttacgc gcactgcgtt caaaatactg tccgagacct ttgcaaaatt ccccaaaatc
      781 ccctaaatgt cttggtggga attttgggga attttgcaaa ggtctcattc tataactgta
      841 aatactttta aatttatgac aaaatagtaa atattgctaa aataatattg atgtcatgaa
      901 attttttcct gctccatgtc tgttggttat cctggctgtc atacccctta aaaccttagc
      961 tgccgatgaa aacgatgcag aacttatccg ttccatgcag cgtcagcagc acatagatgc
     1021 tgaattgtta actgatgcaa atgtccgttt cgagcaacca ttggagaaga acaattatgt
     1081 cctgagtgaa gatgaaacac cgtgtactcg ggtaaattac attagtttag atgataagac
     1141 ggtgcgcaaa ttttcttttc ttccttctgt gctcatgaaa gaaacagctt ttaaaactgg
     1201 gatgtgttta ggttccaata atttgagcag gctacaaaaa gccgcgcaac agatactgat
     1261 cgtgcgtggc tacctcactt cccaagctat tatccaacca cagaatatgg attcgggaat
     1321 tctgaaatta cgggtatcag caggcgaaat aggggatatc cgctatgaag aaaaacggga
     1381 tgggaagtct gccgagggca gtattagtgc attcaataac aaatttccct tatataggaa
     1441 caaaattctc aatcttcgcg atgtagagca gggcttggaa aacctgcgtc gtttgtcgag
     1501 tgttaaaaca gatattcaga ttataccgtc cgaagaagaa ggcaaaagcg atttacagat
     1561 caaatggcag cagaataaac ccatacggtt cagtatcggt atagatgatg cgggcggcaa
     1621 aacgaccggc aaatatcaag gaaatgtcgc tttatcgttt gggcttaagc gatttgtttt
     1681 atgtttcata tggacgcggt ttggcgcaca aaacggactt gactgatgcc accggtacgg
     1741 aaactgcaag cggatccaga agttacagcg tgcattattc ggtgcccgta aaaaaatggc
     1801 tgttttcttt taatcacaat ggacatcgtt accacgaagc aaccgaaggc tattccgtca
     1861 attacgatta caacggcaaa caatatcaga gcagcttggc cgccgagcgc atgctttggc
     1921 gtaacaggtt tcataaaact tcagtcggaa tgaaattatg gacacgccaa acctataaat
     1981 acatcgacga tgccgaaatc gaagtgcaac gccgccgctc tgcaggctgg gaagccgaat
     2041 tgcgccaccg tgcttacctc aaccgctggc aactggacgg caagttatct tacaaacggg
     2101 gtaccggtat gcgccaaagt atgcccgcac ctgaagaaaa cggcggcggt actattccag
     2161 gcacatcccg tatgaaaatc ataaccgccg gattggatgc agcggccccg tttatgttgg
     2221 gcaaacagca gttttcctac gaaaccgcta tccatgcaca atggaacaaa acgcctttgg
     2281 ttgcccaaga caagttgtct atcggcagcc gctacaccgt tcgaggattt gacggtgaac
     2341 agagcctttc cggagagcga ggtttctact ggcagaacac tttgggctgg catttccatc
     2401 cgcgccatca attctatctc ggcgcggact atggtcgggt atcgggcgaa agtgcccaat
     2461 atgcctcggg caagcggctg gcgggtgcag tggccggctt cagaggcagc cataaagtag
     2521 gcggtatatt tgcttatgac ttatttgccg gcaagccgct gcacaagccc gaaggttttc
     2581 agacggccaa caccgtttac ggcttcaatt tgaattacag tttctaaccc tctcctgcat
     2641 tttttaccga cattcagaca gtctttcatc atttacaggc cgtctgaagc gattacaaac
     2701 atcatttaaa gggataaata tgaacaaagg tctgcatcgt accgttttca gcaaaaagca
     2761 caacactatg gttgcagttg ccgaaaccgc caacagtcag ggaaaaggca aacaggcagg
     2821 aacttccgct ccggcttcgc cacaaatttc agacggcctt tacagtaaac tcaaaaccac
     2881 gcttaaaacc ttggtctgct ctttggtttc cgtcagtatg gtattgcctg cccacgcaca
     2941 aatcattacc gataaaaccg cgcccaaaaa ccagcaggcc gttattctta aaaccaatac
     3001 cggtgcgccg ttggtcaaca tccaaactcc taatgcccgt ggattgagcc acaaccgcta
     3061 tacccagttt gatgtcgacg ctaaaggtgc tgtactgaac aacgatcgca acaacaaccc
     3121 gtttctggcc aaaggcagtg cgcagttgat tttgaacgag gtgcgcggtg cggccagcaa
     3181 actcaatggc atcgttaccg taggtggtca aaaggccgat gtgattatcg ccaaccccaa
     3241 cggcattacc gtgaacggcg gcggctttaa aaatgtcggc cgcggtatct taactaccgg
     3301 tgcgccccaa atcggcaaag acggtgcact gacaggattt gatgtgcgtc aaggcacatt
     3361 gaccgtagga gcagcaggtt ggaatgataa aggcggagcc gactacaccg agatacttgc
     3421 tcgtgcagtt gctttgcagg ggaaattaca gggtaaaaac ctggcggttt ctaccggtcc
     3481 tcagaaagta gattacgcca gcggcgaaat cagtgcaggt acggcagcgg gtacgaaacc
     3541 gactattgcc cttgatactg ccgcactggg cggtatgtac gcagacagca tcacactgat
     3601 tgccaatgaa aaaggcgtag gcgtcaaaaa tgccggcaca ctcgaagcgg ccaagcaatt
     3661 gattgtgact tcgtcaggcc gcattgaaaa cagcggccgc atcgccacca ctgccgacgg
     3721 caccgaagct tcaccgactt atctctccat cgaaaccacc gaaaaaggag cggcaggcgc
     3781 atttatctcc aatggcggcc ggatagagag caaaggctta ttggttattg ataccgggga
     3841 ggacatcggt ttgcgtaacg gagcggtcgt tcaaaacaac ggcaaccgcc ctgctgctac
     3901 ggtattaaat gccggccgta atttggttat cgaaagcaaa gcaaatgtga acaatgctaa
     3961 aggttcgacc aatctgtcgg ccggcggtcg tactacgatc aatgatgcta ctattcaagc
     4021 gggcagttcc gtgtacagct ccaccaaagg cgatactgaa ttgggtgaaa atacccgtat
     4081 tattgctgaa aacgtaaccg tattgtccaa cggcagtatt ggcagtgctg ctgtaattga
     4141 ggctaaagac actgcacaca ttgaagcggg taaaccgctt tctttagaga cttcaaacgt
     4201 tgcttctaac atccgtttga acaacggtag cattaaaggc ggaaagcagg tggtattgat
     4261 ggctgatgac gacattcagg caaaagcctc ccacctgaat gcgtccggca atctgtatat
     4321 tcatgcaggt aaggatctgg atttgaatgc cgataaagac ttatcaacac aaagtatcag
     4381 cctgagggca gacaacaccg ccctcatcag cagcaacggt aacaccttga ctgcagaaaa
     4441 gaatctggat attcaggcag gtagcttgag tgtgcgtcaa agtaatctgc aatccagtgg
     4501 cggtaatgtg cagatgagcg cgactaaagg caacatcagc ttgaatcaga gttggataaa
     4561 tgccagtcag aatattgata cggcagcact tcaaggcaat attatttcag acggtctgac
     4621 tgctgttgcc gaagttggac gcgtatctct tcttgccaac ggcaatgttg atttcaacgg
     4681 tctaaatacc ttgattgcgg aaggagacat taatgctggt tcagtcggta aaggccgtct
     4741 gaaaatggac aataccgata tttatgcttc tgcaggcgat gtgaaactgg ttgccggagg
     4801 tcaattagat ttaggcaacg gcaccgttaa cggcggtcat atcagcttag acagcaataa
     4861 aggcagcatg gtggtgcagg atgtacacct gaatgcccgc gcatcactga aagtggatgc
     4921 ggatcaaact ttaaccatta ataacagcaa gctcaattcc ggtcacaata cccagattaa
     4981 tacaaatcat ggtcatatga cgcttaatca gcttgatgct cattcacgtc gtcatatgag
     5041 tatcagtgca cagggtaaag gcaaaggaaa agacagcggt caaattttac aaaacgacca
     5101 gcaaaacagt aaaagtactt tggcggcaga tggtgtattg tcattgaaca gcagcgcatt
     5161 acaggttttg gacaatacta ccctgcgcgg tggcgcgata aacatcaaag ctggcggagg
     5221 catcatcaaa cgggggcata tcgattggga aacccaagat acggcaacta tgcgttcagc
     5281 ggaactgaaa ccgctgtccg gtatgatgtc gatagaatcg ggcggtaata accctttaac
     5341 cgttgaaccc ggtaaccgta tcgtttctgc aggtgatttg gccgtgaacc acaacggcac
     5401 attccaaatc agtgccagag caggaaataa cggcaatccg agtgcgcaaa cagccagcgt
     5461 ttcagctaaa ggcaatatcg ggattgtggc gggagaggtg gatattgatg cagcaaatat
     5521 tgccgcaggg aaagatttgg ctttagtggc aactaaaggg aatatttcac taaacagtat
     5581 taggaatacg tttagtaact atcaattgaa aacagataaa cacaatatca cacagcaact
     5641 tactgatgta gagcaggagt ttagtaagtt aacgagcgat cctaaatatc gtaaagcaca
     5701 ggatctttca caaatgttga ggcgtaaata taagaggcgt gacaaagtat tcggtgatag
     5761 tgaggccaga ttacgtggtt tacgagcaaa gataaatgct gctgatgagg cttgggcaga
     5821 acgccaatcc ccagtaaaag cgttacttga acgtaaacaa ttgttacaac aagccctttt
     5881 aacagtttcc caaccgggta gtggtcacga aaaccaaggt agcaccctta gcggtcaaaa
     5941 tatcaaactg ctcgctgcag gtggtatccg tatccagggc tccaaagttg ctgccaccca
     6001 acaggcaaat atccaagctg caggattttt acctgcacca gccgcagagg aactgcaaga
     6061 aggtcgactt caatcagcca ttgacatcag cggtgtattt gatacttttg aatatggtca
     6121 gcaaggcagc gataaatacg gctatgccat tttcagcagg ccatctgaaa tttcaggaaa
     6181 aacaggtgtt accctctctg caccaaatgc caatgagaac agccgcatca gtctgagtgc
     6241 ggcaaacatc gaagctgaaa acggcaaaat caaaattcaa tcttacggtg accaatatta
     6301 ctatgcgaga cagagcgaac tctatacctt tgaacgccgc agctacaaaa ccggcaaatg
     6361 gtacaaccgc aaacacatta ccgaagtcaa agaacacaaa aacgccaagc ccgacgcagt
     6421 aaccctcagc gcatcccaag gcatcgacat caaatctggt ggcaacatcg acgcctacgc
     6481 caccgcattc gatgccccca aaggcagcat taacatcgaa gccgggcgga aattgacact
     6541 ctatgccgta gaagagctca actacgacaa actagacagc caaaaaaggc gcagatttct
     6601 cggcatcagc tacagcaaag cacacgacac caccacccaa gtcatgaaaa ccgcgctgcc
     6661 ctcaagggta gttgcagaat cagccaacct ccaatcgggc tgggatacca aactgcaagg
     6721 cacacagttt gaaaccacac tgggtggcgc aaccatacgc gcaggcgtag gcgagcaggc
     6781 acgggcagat gccaagatta tcctcgaagg gatcaaaagc agcatccaca cagaaaccgt
     6841 gagcagcagc aaatctactc tatggcaaaa acaggcagga cggggcagta acatcgaaac
     6901 cttgcaattg ccgagtttca ccggtcccgt tgcgcccgta ctgtccgcac ccggcggtta
     6961 cattgtcgat attcccaaag gcaatctgaa aacccaaatc gaaaccctca ccaagcagcc
     7021 cgagtatgct tatttgaaac aacttcaagt tgcgaaa
//
LOCUS       DAWKOG010000103         7056 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.103, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000103 DAWKOG010000000
VERSION     DAWKOG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7056
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.103"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            144..725
                     /locus_tag="WJK66_001510"
     CDS             144..725
                     /locus_tag="WJK66_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ7579211.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLAGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(787..1641)
                     /gene="folD"
                     /locus_tag="WJK66_001511"
     CDS             complement(787..1641)
                     /gene="folD"
                     /locus_tag="WJK66_001511"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEZ7579212.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQDNLHTPCLAVVLVGGD
                     PASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLTDEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            1864..1941
                     /locus_tag="WJK66_001512"
     tRNA            1864..1941
                     /locus_tag="WJK66_001512"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1899..1901,aa:Pro,seq:tgg)
     gene            1966..2042
                     /locus_tag="WJK66_001513"
     tRNA            1966..2042
                     /locus_tag="WJK66_001513"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2000..2002,aa:Arg,seq:tct)
     gene            2070..2145
                     /locus_tag="WJK66_001514"
     tRNA            2070..2145
                     /locus_tag="WJK66_001514"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2103..2105,aa:His,seq:gtg)
     gene            2280..2786
                     /locus_tag="WJK66_001515"
     CDS             2280..2786
                     /locus_tag="WJK66_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional heptose 7-phosphate kinase/heptose
                     1-phosphate adenyltransferase"
                     /protein_id="HEZ7579213.1"
                     /translation="MVDAWSVPDFESKICPPESLAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSMGGALVLALNTDASVRRLGKGGDRPVNPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            2783..4561
                     /locus_tag="WJK66_001516"
     CDS             2783..4561
                     /locus_tag="WJK66_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HEZ7579214.1"
                     /translation="MTVLKPSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLKTQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLVELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGKQTVVSGEISLRPDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHT"
     gene            4674..5498
                     /locus_tag="WJK66_001517"
     CDS             4674..5498
                     /locus_tag="WJK66_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEZ7579215.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRAVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(6260..7048)
                     /locus_tag="WJK66_001518"
     CDS             complement(6260..7048)
                     /locus_tag="WJK66_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEZ7579216.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRIAQPAMITVSLRR
                     TGSGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDVGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARDKAQASTPTVGQPFWHSAEY"
BASE COUNT         1508 a         1827 c         2123 g         1598 t
ORIGIN      
        1 aattatgtat ggcgtaaagg aagcgtttta acgccgtatt gggaaaatgt aaagttcttt
       61 atcgggattt ctttaagtat tggcgttttc ggtgtcgtgg cggtagaatc ggggtcagtt
      121 aagaacaatt atcaggaacg gatatggaag aaaaaaacga ctacaccgac gcggcatccg
      181 acaaccgcaa cgggcaggaa attgaagtcg gaattgccgg cgcaggcgac cggatacttg
      241 ccgcgctgct gaaccaattg tttacctttt tggttttgtt ggtgccgttt gccggactga
      301 ttgcttttgc catcaagaat gaaggcagga ttggcggcag tgaggaaata ttcgggctgc
      361 ttttgggcat gacttccttt tgggtaggac tggcgggcat tttggtttat accgtcgttc
      421 aaatctacta tatgagtcgg gacgggcagt cattgggtaa gaaaatcatg agaatccgcg
      481 tgttgaaaac cgacggccgc aatcccggtt ttgtcggcac ggttttggta cgcgaaatcg
      541 catggtcggt tttggttgcc attattgccg ccgttatcgg tcttgcagta ggtgacaacg
      601 gagaaaacgc catcaacctg ctggcattcc ttgccaactt tgtcctgctc tttatggtca
      661 aacgcgaccg ccgcacgctt tacgacatac tggcggatac ggttgtcgtc aagctgccca
      721 aataaacgga cacggcaaaa tgccgtctga aagcctttca gacggcattt ttatcttcag
      781 aaccgctcaa gcatcgtgca gtgaagccgc gtgcagggtg ttttccatca atgtggcaat
      841 cgtcatcgga cccacgccgc cgggaacggg cgtaatcatc gccgcccgtt cttttgccgt
      901 ttcaaattcc acgtcgccgc acaggctgcc gtcgtccaaa cggttgatgc ccacatcgat
      961 aacgaccgcg ccgggtttga tccattcgcc tttgacaaag ttcggaatgc ccacgccgac
     1021 caccaaaata tcggctccgg caacctcgtc tgtcagattt tcggttgcgc tgtggcagac
     1081 cgttaccgtt gcgcgcgcca gcagcagttc caaagcctgc gggcggccga cgatattcga
     1141 cgcgccgacc acgaccgctt ttttcccctt cggatcaatg ccgtaagctt ccaaaagcgt
     1201 catcacgccc ttgggcgtac acgggcgcat cagcggcatt ttgaccgcca gcctgccgac
     1261 attgtaagga tggaagccgt ccacgtcctt atccggcgaa atacgttcca aaaccgcctg
     1321 gctgtcgagg tgcttcggca ggggcagctg aaccagaata ccgtccactt cggaatcggc
     1381 attcaggcgg tcgaccagtg ccagcagttc ttcctgcgat gttgattcgg gcagttcgta
     1441 agacagtgat ttgatgccgc atttttggca ggcagttttc ttgttgcgga cataaaccgc
     1501 gctggcaggg tcgccgccga ccaaaaccac ggccaggcaa ggcgtgtgca gattgtcctg
     1561 ttggcgttgc gccaccgctt cggcaaccgc ctgcaggcgt ttttgcgaaa cttctttacc
     1621 attgatcagt tgtgccgaca tatccgtcct tttgttaaac aggtagaaaa tgctgaaaat
     1681 tatcgcattt tccgtttgtt tttacagcag gatgccgaaa ataaatttgc cctgaaataa
     1741 aatacggctg tttggacgga ttaaatattt tttcaaatca aaaaactata tttttcatct
     1801 tggtgtcttg aaaataaata ttgacgcatc tttccctgag ttgtatagtt cgcttcttca
     1861 agtcggggcg tagcgcagcc cggttagcgc atctgctttg ggagcagagg gtcgtgagtt
     1921 cgaatcccac cgccccgacc aatcaatcag cacgaatcgg catgagcgcc cgtagctcaa
     1981 ccggatagag caccgacctt ctaagtcggg ggttacaggt tcgattcctg tcgggcgtgc
     2041 caagaatttg attgaaaacc gtttttatgg tggctgtagc tcagttggta gagccccgga
     2101 ttgtgattcc ggttgtcgtg ggttcgagcc ccatcagcca ccccagataa aggcccgtac
     2161 ttttcggtac gggtcttttc tcgtttgcgc gtactgtccg cgccgccggc tttgcctttc
     2221 gcatatgctt ctgtatcatg taggttttct gtcttttatg gtttttgtcg gaggctttga
     2281 tggttgacgc ttggtctgtc cctgattttg aatcgaaaat ctgtccgccc gagtcgttgg
     2341 cggcgcgttt ggcgttgttg ccgcgcccgc tggtgtttac caacggctgt ttcgacatcc
     2401 tccacagggg gcatgttact tatctggcgc aggcgcgttc gatggggggc gcgttggtgc
     2461 tggcgttgaa taccgatgct tcggtgcggc gtctgggcaa gggcggtgac cgcccggtta
     2521 atcctttgga gaaccgtgcc gccgttgccg ccgcgttgga aagtgtggat ttggtaacgt
     2581 ggtttgacgg ggatacgccg gcggcgttga ttgaggcggt caaacctgaa attttggtca
     2641 agggcggcga ttgggctgcg gataagattg tcggtgcggc agaaacgttg gcgcgcggcg
     2701 gtcaggtgtt ttcaattccg tttctgcacc agacttcgac aacgaagact ttggcaaaaa
     2761 tccgtgcggc agagggcgga aaatgacggt tttgaagcct tcgcactggc gggtgttggc
     2821 ggagcttgcc gacggtttgc cgcaacacgt ctcgcaactg gcgcgtatgg cggatatgaa
     2881 gccgcagcag ctcaacggtt tttggcagca gatgccggcg cacatacgcg ggctgttgcg
     2941 ccaacacgac ggctattggc ggctggtgcg cccattggcg gttttcgatg ccgaaggttt
     3001 gcgcgagctg ggggaaaggt cgggttttca gacggcattg aagcacgagt gcgcgtccag
     3061 caacgacgag atactggaat tggcgcggat tgcgccggac aaggcgcaca aaaccatatg
     3121 tgtgacccac ctgcaaagta agggcagggg gcggcagggg cggaagtggt cgcaccgttt
     3181 gggcgagtgt ctgatgttca gttttggctg ggtgtttgac cggccgcagt atgagttggg
     3241 ttcgctgtcg cctgttgcgg cagtggcgtg ccggcgcgcc ttgtcgcgtt tgggtttgaa
     3301 aacgcaaatc aagtggccaa acgatttggt cgtcggacgc gacaaattgg gcggcattct
     3361 gattgaaacg gtcaggacgg gcggcaaaac ggttgccgtg gtcggtatcg gcatcaattt
     3421 cgtgctgccc aaggaagtgg aaaacgccgc ttccgtgcaa tcgctgtttc agacggcatc
     3481 gcggcgggga aatgccgatg ccgccgtgtt gctggaaacg ctgttggtgg aactggacgc
     3541 ggtgttgttg caatatgcgc gggacggatt tgcgcctttt gtggcggaat atcaggctgc
     3601 caaccgcgac cacggcaagg cggtattgct gttgcgcgac ggcgaaaccg tgttcgaagg
     3661 cacggttaaa ggcgtggacg gacaaggcgt tctgcacttg gaaacggcag agggcaaaca
     3721 gacggtcgtc agcggcgaaa tcagcctgcg gcccgacgac agaccggttt ccgtgccgaa
     3781 gcggcgggat tcggaacgtt ttctgctgtt ggacggcggc aacagccggc tcaagtgggc
     3841 gtgggtggaa aacggcacgt tcgcaaccgt cggtagcgcg ccgtaccgcg atttgtcgcc
     3901 tttgggcgcg gagtgggcgg aaaaggcgga tggaaatgtc cgcatcgtcg gttgcgctgt
     3961 gtgcggagaa ttcaaaaagg cacaagtgca ggaacagctc gcccgaaaaa tcgagtggct
     4021 gccgtcttcg gcacaggctt tgggcatacg caaccactac cgccaccccg aagaacacgg
     4081 ttccgaccgc tggttcaacg ccttgggcag ccgccgcttc agccgcaacg cctgcgtcgt
     4141 cgtcagttgc ggcacggcgg taacggttga cgcgctcacc gatgacggac attatctcgg
     4201 gggaaccatc atgcctggtt tccacctgat gaaagaatcg ctcgccgtcc gaaccgccaa
     4261 cctcaaccgg cacgccggta agcgttatcc tttcccgacc acaacgggca atgccgtcgc
     4321 cagcggcatg atggatgcgg tttgcggctc ggttatgatg atgcacgggc gtttgaaaga
     4381 aaaaaccggg gcgggcaagc ctgtcgatgt catcattacc ggcggcggcg cggcaaaagt
     4441 tgccgaagcc ctgccgcctg catttttggc ggaaaatacc gtgcgcgtgg cggacaacct
     4501 cgtcattcac gggctgctga acctgattgc cgccgaaggc ggggaatcgg aacatactta
     4561 aaaaatacac cctttttcaa cacggggcgg catccgtccg aatccgtccg gattctgccg
     4621 cccgacagac tgccgcccag gcgggcgggg tttcaaacat tgaggaaatt ctaatgaaat
     4681 ggctcttcac ccttttggcg gtactcaata ttgccgtatt cggcggcacg gtgggctata
     4741 aattggccgt taaagcgacg ggcggcgtgc cggaaaaccg ggccgtcgaa aacacgcccc
     4801 ccgcaacgcc cgccgccgac aatgcggcgg catccgtgga ggatacggcg gcactgctca
     4861 aaccgggcga catcctgacc gaagagcagg ccgagcagtt gcgcttgaaa aaggaagcgg
     4921 aacagaaaaa actgagggag aaaaaacagc gtgaagaaaa agcccgccgc gaaaaactcg
     4981 ccgccgaaaa ggcgcaggcg gaacgcgaaa acggcgcggc ggatgcctta tgcgccgcgc
     5041 aggcaagcct cacgatggac gaagacgact accaccgcat caaagggctt ttgggcaaat
     5101 ggtcgcacgt tgccagcagg agcgttgaaa aacgcaccgc caaagccaaa cctgccgaca
     5161 aaacctaccg cgtcgtcctg cccgtttccg ccgatgccga aaatcaggcg gcggagctgt
     5221 ctgccaaagg tttcaacccc ataccgtttg acggcgcatt gagtttgggt gtcggcaaca
     5281 gtcgggaaaa cgcccaagcc ctgcaaaacc ggcttgccgg cgccggattc ggcggggcgc
     5341 atattgtcga acactttgcc gaagccgaca ggcaggacga ttctttgagc gtgtcgcgta
     5401 tgacggtttt gtttaccggc gtgaatgccg ccgatgcgga cgaaatccgt aaaatcacgt
     5461 ccctatacgg caaactgaac ctcaagtctt gcaaataggc gcgaaagccg gacgataaac
     5521 gccaaaccca ccgcgaaggt gggtttggcg tttcggtttt gagtatctga tatataaaaa
     5581 gggaatgtat gtctcgtata tgccgactcc aagtgtgaaa gtgatgatgg ggaaatatgg
     5641 tgtttaatgg ttcggaacgg acggcttccg agtggttttg attatacgct aataataata
     5701 attatcaata aaaatttaaa aataaatgca gattttttgt aatacgatga aaaataaaca
     5761 aattaacctg tccgccgatg tttgtgattc gggtcggcaa actttcattg tgtcggacaa
     5821 atcggggtcg gcaggcatag ctgagggctt cgtgcctgac ggaaataagc ctgccgtgtt
     5881 tgaacatccg tccgatttgg cgcggacagg gttgtcattc gggggtgttt gagcgtggat
     5941 tcggggatgc aaaagcggta aaaccgttgc ggatgaaggc ttacggacgg ctttaggtat
     6001 ttttaagtgt attcaaacaa caacaaaacg cttcttctat tgccgtattt agggcttgcg
     6061 cggcagcctg cacaccgtgg aggccgagtg tttcgttggt tgcggcatcg ggacgggcgg
     6121 caaggttttc cggccggttt ttccattttt tccgacagag ccgtgatgct tgggaatggc
     6181 ggtttgcccg tttcagaccg ccgccgtttt gggctgcgat gccgtctgaa ccgttcagac
     6241 ggcatttttg ccgccttttt caatattccg ccgaatgcca aaacggctgt ccgactgtcg
     6301 gcgtgctggc ttgcgctttg tcgcgtgctt cgaccggtcc ggcttcaaat gccagccgtc
     6361 cggattcgac ggcgagtgcg aaggcgcgtg ccatattgac cggatcgccg ctgcgggaaa
     6421 cggcagtatt caaaagcacg ccgtcaaaac cccattccat cacttgtgcc gcctgtgagg
     6481 gcaaacccaa gcccgcgtcg ataatcagcg gcgtgtcggg caggcgttcg cgcaggacgt
     6541 tcaacgcgta ggcgtgaacc gcgcccaaac ccgtgccgat cggagccgcc cacggcatta
     6601 acgcctgaca gcccacatcg agcaggcggc ggcaggcaat caggtcttcg gtgcaataag
     6661 gcagcacttt gaagccgtct ttaatcagga tttccgccgc ttcgacaagt tggaacacat
     6721 ccggctgcaa ggtgtcgtcg tcgccgatga gttcgagttt aatccaatcg gtttcaaaca
     6781 cttcgcgcgc catttgcgcc gtcgttaccg cttcctgcac gctttggcag cctgccgtgt
     6841 tcggcaggac gggaacgccg gtttcttgaa gcagcgacca aaacccctga ccgtgcgcct
     6901 cgccgccgct tcccgtgcgg cgcagcgaga cggtaatcat cgcgggttgg gcgatacgga
     6961 cggattgttt gaggatttca ggggtcgggt aggcagccgt gccgagcagc agccgtgaag
     7021 ggaaagtttc tccgtatagg gtgagcataa tgggtt
//
LOCUS       DAWKOG010000104         6997 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.104, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000104 DAWKOG010000000
VERSION     DAWKOG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6997
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.104"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(74..1495)
                     /locus_tag="WJK66_001519"
     CDS             complement(74..1495)
                     /locus_tag="WJK66_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEZ7579217.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWSLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGSLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(1853..2212)
                     /locus_tag="WJK66_001520"
     CDS             complement(1853..2212)
                     /locus_tag="WJK66_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEZ7579218.1"
                     /translation="MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(2209..2814)
                     /locus_tag="WJK66_001521"
     CDS             complement(2209..2814)
                     /locus_tag="WJK66_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEZ7579219.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDSFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVIENGLEGIVQIAPFHPQFRFQNTEPDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDTSAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(2861..3343)
                     /locus_tag="WJK66_001522"
     CDS             complement(2861..3343)
                     /locus_tag="WJK66_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEZ7579220.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      3547..3644
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3855..4955
                     /gene="gcvT"
                     /locus_tag="WJK66_001523"
     CDS             3855..4955
                     /gene="gcvT"
                     /locus_tag="WJK66_001523"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEZ7579221.1"
                     /translation="MITLKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVSFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVVHNLKPFQGADLGNDWFVARTGYTGEDGVEVILPGIEAVVFFKALQQA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLDKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLMRGKEADVRVLKLPFVRNGQKQFD"
     gene            5117..5503
                     /gene="gcvH"
                     /locus_tag="WJK66_001524"
     CDS             5117..5503
                     /gene="gcvH"
                     /locus_tag="WJK66_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEZ7579222.1"
                     /translation="MSNNIPAELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNDDLPSAPETANSDPYGAGW
                     FFKIKPANPADYDGLLTAEQYAGEVD"
     gene            5649..6896
                     /locus_tag="WJK66_001525"
     CDS             5649..6896
                     /locus_tag="WJK66_001525"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEZ7579223.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAACLPEAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEISPYLYTLGMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRKSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
BASE COUNT         1785 a         1682 c         1953 g         1577 t
ORIGIN      
        1 gtcggaaggg gggatattga tttgattatg ccggaattta aaatgccgtc tgaatgttca
       61 gacggcatag cgtttacagc agtttgaaaa cgaaaaagat aagggtatgt acgatgaaga
      121 cgggtgtcag gaaggcgacc gaccacatca tatagccgaa gaaagtcggc atcggtacgc
      181 cgcgctgttc ggcaatggcc ttgaccatga agttcggtgc gttgccgatg taggtcagtg
      241 cgcccatgaa taccgaaccc atagaaaccg ccagcagcga atgaaacagg gaacccgtca
      301 tcaaggcttg ggcatcgccg cccgccatat tgaaaaaaac gagataagtg ggcgcgttat
      361 ccaagaatgc cgacaatatg ccgctcatcc aaaaatacat cacattaatc ggatgacctg
      421 ccgtatcgtg aaccagcgat accaccccgc ccagcgcgcc tgcctcgcct gctttcagaa
      481 tgctcaggac gggaaagatg gtgatgaaga tgccgaggaa gagtttgccc acttcggcga
      541 tgggttcaaa gttgaattcg ttgcctgcgc ggacttgttt gggcgtgatt gccatagata
      601 cggcggtcaa tgcaatcagg atgacatcgc ggacgaggtt ttgcagggcg taacggctgc
      661 cgaggatttc aaatcccggg tgttcgggtt tccaaaggcc ggacattaga accgcgccga
      721 ccacgcccga aagcaggagg aagttccatt tgccgaagat ggcgattttt tcgggttttt
      781 cctgttgtgc cggcgtatct tgtgcaatgc tttcctgttt gaagaaacgg ttgtcgatga
      841 aatagaaggc ggtcaacagg acagcggtgc tgatcaggac gggggcgaac atatgtttga
      901 ccgtccacat gaaatctacg cctttgagga agccgaggaa gagtgggggg tcgcccaaag
      961 gggtcagacc gccgccgatg tttgcaacca ggaaaatgaa gaagatgacg atgtgcacgc
     1021 ggcgggtacg gttttggttg gctttcagca gcggacgaat catcagcatt gctgcgccgg
     1081 tcgttcccat gatagaggca agtgccgtac cgacggcaag cagggcggtg ttgagcttgg
     1141 gtgtgccgtt caagtcgccc caaaccaaaa tgccgcctga aatggtgtac agggcaagca
     1201 gcagcaggat gaaagggatg tattcttcaa cgagtgcgtg tgcgacggta tggataccgg
     1261 cggacgcgcc aaaaaccaaa ctgaacggga tgaggaagag caatgtccaa aaggcggtaa
     1321 ttttgccgta atggtgatgc caggtatgcg aaaaaaacaa gggacccaat gcgatagaca
     1381 gcaaaatcag ggcaaagggc aggctccaca gcaggtttag gtttgcgccg tccaaatctg
     1441 cggcgtaaac cgatgcgggg aaaagcatta gtgaaaacag gggtaggtgg cgcatcgtgt
     1501 ttcctcgatt caagcactgc cttgcgcggc gcgtgggagt gatacaggca ccgtgccgcc
     1561 cggacatagg cggactgtgc tgcctgtttg tttttgaaaa aagttttccg attgattgta
     1621 aagtaaataa tcaggataaa ccagtttccc aaaccggaag gcggcgggaa ggcggattgc
     1681 tgtgcttggg aatatatgtt ctttttgata aataatttta tttaaaacaa ataaatatat
     1741 gtctttaata aataatctat cgaaaacgaa aaatgaattt attttaacat atatttgcaa
     1801 tgaaacaggt ttgccccccg tttgtttgcc cttatccctt tcagtacggc attcaagatt
     1861 cgggcctgcg ccacatccat atggcgacaa gggaacaaaa aaccgatgaa accgccccga
     1921 cccaccagcg ttggggaaac tgccaaaaca ttatcaggca ggatgcggtc atcatactga
     1981 tggcgaatat tttggctttg cgcggcactg cgccgttttg ttcccagtta tgaaccatcg
     2041 gaccgaaata gcggtgccgg tgcagccagc ggtgaaagcg cggggatgcc tttgcccagc
     2101 aggcggcgga gagcagtacg aacggcgtgg tcggcaacag cggcaaaaaa atgccgatga
     2161 tacccaacag cagggaaatg cagccgcagg caattaaaag ataacgtatc attttgaaat
     2221 atttttctta ttgtgcggat aagggcagga tgtgataccg agttttgccc agccttcatg
     2281 tcccattttt tccagcaggg cgatattgcg ttcgaatatt gccgaagtgt cgggaaaggc
     2341 ttgtgcggct ttggcaatgc tgtcttcgcg gatgaggtgc agcgtcggat agggagaacg
     2401 gttggtgtag ttgccgatgt cgtcgggttc ggtattctga aagcggaatt ggggatggaa
     2461 cggtgcgatt tggacgatgc cttccaagcc gttttcgata acggcggcat cggcaatatc
     2521 gagcatatcg ttgaatacgt cgaaatcggg gaatagggtc gggtgaacca gcagggtggt
     2581 ttccagttcg gtggcgggtg tattgcccag tcgctgcagt tcttcgtcca agtcttccaa
     2641 aaaactgtca aggtgtttgg cttcgctgat tgcgatgcgg acaaggtttt taacgtgggg
     2701 ggctttggca aagggacaca ggttcagacc gatgacggct ttttccaacc attgtccggt
     2761 gtgttcggca acagcatctt tattttcgga agtattgata ttcattattg tcatgtaaat
     2821 gtgtttgcag attgcacgtg cgggaaaatc gggaagggca ctattccttc agcaggtggt
     2881 tgagcggcag ggaggtggtg tgtttgattt cttttaaaac aaagctcgat tgcgcatctt
     2941 gtacgccgtg gtgggacagg agcgtatcca aaacaaaatg ggaaaacgcg ttcatatcgg
     3001 taaaaaacgc ctgaagcagg tagtcggttt cccctgtcag ggcgaagcag ctcaagactt
     3061 caggccattt tcggaccgat gcggcaaagt cttcccgcgc gtcttttgct ttgcggatgg
     3121 aaacgcggat aaatgcctga agccccaggt tgacggattc cggagacagc agcgcggcat
     3181 attggcggac gatgccggca tcttccaact gctttagacg gcgcaggcac ggagaaggcg
     3241 aaagtgcgac acgttcggac agttcgacat tggtcagcct gccgttttcc tggagaacct
     3301 gtaagatttt aatatcggtt ttgtctaaag tgagttgggg catatttgcg ttccgtttta
     3361 aggaattcgg attgtctgtc cgtatgtttg cggcaatccg cacagatgga gaccatatta
     3421 acatataaaa agttataccg tcatccggga caaattttgt tttcggaaaa tcatgtgaaa
     3481 acagaggcgg tcggtttgca tctcttttaa gacggcttgc ccaaaccgcc gattcaagac
     3541 ataatcggga aatgtgcagg agagtgttac acccaactac aatgtaacca ccgaaggcgc
     3601 agacaccctt aaatcgctca ggtatcaggg actgcacatt gaaacaaaca atctggagag
     3661 cggcgttgga ataacgtcca ccgaagggga gaaggccgtc tgaaccacca ttcagacaac
     3721 cgcgcaaagc agtgagcaga ctggtttgcc atcatgcgga tacggccgaa aatctcaggt
     3781 tcaaggacag atagggtcat ccgcgcacag gtgcgcgggt ggcatctgaa caaaaaatcc
     3841 ggagaaactt gagaatgatt actctgaaaa ccaccccatt tcatcaagcc catcaagatg
     3901 caggcgcgaa gccggtagat tttgccggct gggagctgcc catccattat ggttcgcaaa
     3961 tcgccgaaca cgaagccgtg cgcaccgacg ccggtatgtt tgacgtatcc catatgcttg
     4021 ttaccgacgt agcaggcgca aatgccaaag cctttttccg caaattgatt gccaacgatg
     4081 tcgccaagct cgcttttgtc ggcaaagccc tttattccgc tttgctcaac gacaacggcg
     4141 gcgtgattga cgacttaatc gtttaccgca ccaacgaagc cgaaacccaa taccgcatcg
     4201 tgtccaacgg cgcgactcgc gaaaaagata cggcacaatt ccacaaagtc ggacaagagt
     4261 tcggtgtttc cttcaatccg cgctacgacc tcggcatgct cgccgtacaa ggtcctaaag
     4321 ccattgaaaa actcctgacc gtcaaacccg aatgggcaga tgtcgtccat aacctcaaac
     4381 cgttccaagg cgcggatttg ggcaacgact ggtttgtcgc ccgcaccggc tacaccggcg
     4441 aagacggcgt cgaagtcatc ctgcccggca tcgaagccgt cgtattcttc aaagccctgc
     4501 aacaagccgg cgtacagccc tgcggcctcg gcgcgcgcga taccctgcgc atggaagccg
     4561 gtatgaacct ctacggcaac gatatggacg acgacaccag cccgcttgaa gcaggcatgg
     4621 gttggaccgt tgatttgaaa gacgaaagcc gcgatttcgt cggtaaagcc gccttgctgg
     4681 cattgaaaga aaaaggcgtt gccgtcaaac aggtcggtct gttgctcgac aaaggcggca
     4741 tcctgcgcgc gcatatggaa gtgttgaccg acaaaggcaa aggcgaaacc accagcggcg
     4801 tcttctctcc aagcctgaaa caatccatcg ccatcgcccg cgtgccgaaa gactttgacg
     4861 gcgataccgc caaagtgctg atgcgcggca aagaagcgga cgtgcgcgta ctgaagctgc
     4921 cgttcgtgcg caacggacag aaacagtttg attgatgcgg tttcagacgg cattttcatt
     4981 tcatatgccg tctgaaagca ggttttaatt gttgtccgat acggacgttt gtagaaagca
     5041 ttgaacaagg catctgtgga tattgattca tgcagatgcc gtctgaaaat aacccctatc
     5101 aatggagtat caaaccatga gcaacaacat cccggccgaa ctgaaatacg ttgccagcca
     5161 cgaatggctg cgccttgaag aagacggtac catcaccgtc ggtattaccc accacgcgca
     5221 agagctgttg ggcgacatcg tgttcgtcga gctgcccgaa gtcggcgcaa accttgccgc
     5281 tgaagagcaa gccggtgtgg ttgagtctgt aaaagccgcg tccgacgtgt acgcaccgat
     5341 tgcaggtgaa gtcgttgccg tcaacgatga cttacccagt gcacctgaaa ctgccaacag
     5401 cgacccttat ggcgcaggct ggttcttcaa aatcaaacct gccaatcctg ccgattacga
     5461 cggtctgctg actgccgaac aatacgcggg cgaagtagat taaattccta tcctgataat
     5521 aggatgcctt atttaaggaa ctgcactttc agacggcaag gatgcgtttg gaagtgcagt
     5581 ttttgtctgt gtcttcaagg tcagttaggc tataataacg gtctatattc atctttaccg
     5641 attttttcat gcaacttacc gctgtcggac tcaatcatca aaccgcacct ttaagcatac
     5701 gggaaaagct ggcgtttgcc gcggcctgcc tgcccgaagc cgtccgcaat cttgcccgaa
     5761 gcaatgcggc aacggaggcg gtaatccttt ctacctgcaa ccgcaccgag ctttactgtg
     5821 taggcgattc ggaagaaatc atccgttggc tcgcagacta tcacagcctt cccatagaag
     5881 aaatcagccc ctacctttat actttgggga tgcaggagac tgtgcgccat gctttccgcg
     5941 tcgcctgcgg cttggattcg atggtgttgg gcgagccgca gattttagga cagattaagg
     6001 atgcggtcag ggttgctcaa gagcaggaaa gtatgggtaa gaaactcaat gccctgttcc
     6061 aaaaaacctt ttctgttgct aaagaggtcc gtaccgatac tgccgtcggc gaaaactcgg
     6121 tttccatggc ttccgcttcc gtcaaattgg cggaacagat ttttcccgac atcggcgatt
     6181 tgaatgtctt gtttatcggc gcaggcgaaa tgattgagct ggttgccact tattttgccg
     6241 ccaaaagtcc ccggctgatg acggttgcca accggacgct ggcgcgtgca caggagttgt
     6301 gcgacaagct cggtgtcaac gccgaaccgt gcctgctgtc cgatctgcct gccattctgc
     6361 acgattacga cgtagtggtt tcttcaacgg caagccagtt gcccattgtc ggcaaaggca
     6421 tggtggagcg tgcattgaaa caaaggcaga gtatgccgtt gttcatgctt gatttggcag
     6481 tgccgcgcga tattgaagcg gaagtcggcg atttgaacga tgcctatctt tatacggtgg
     6541 acgatatggt caacatcgtc caaagcggca aggaggcaag gcagaaggcc gccgccgccg
     6601 ccgaaacgct ggtgtccgag aaggttgccg aatttgtccg gcagcagcag ggcaggaaga
     6661 gcgtcccctt gattaaagcc ttgcgggacg agggcgagaa agcgcgcaag caggtgttgg
     6721 aaaatgcgat gaaacagctt gctaaaggcg caacggcaga agaggttttg gaacggctgt
     6781 ccgtccaact gaccaacaag ctgctgcatt cgccgaccca aactttgaat aaggcggggg
     6841 aagaagataa ggatttggtt catgccgtcg cgcagattta tcatttggac aaataacggt
     6901 gcgccgggaa aaatgccgtc tgaagaggtt tcagacggca tttttttgtg ccgcctgaca
     6961 acatcgtaaa atcccacatt atatcgatgt aatcaca
//
LOCUS       DAWKOG010000105         6782 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.105, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000105 DAWKOG010000000
VERSION     DAWKOG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6782
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.105"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(75..1421)
                     /gene="mnmE"
                     /locus_tag="WJK66_001526"
     CDS             complement(75..1421)
                     /gene="mnmE"
                     /locus_tag="WJK66_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEZ7579224.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAEALCGKTPKP
                     RTATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRAILDALPPELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGESEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1552..1707
                     /locus_tag="WJK66_001527"
     CDS             1552..1707
                     /locus_tag="WJK66_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579225.1"
                     /translation="MEKRNTYALLLGIGSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1704..2135
                     /locus_tag="WJK66_001528"
     CDS             1704..2135
                     /locus_tag="WJK66_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HEZ7579226.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARRFHSGP
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISVILIL
                     LFAVFLVWSGYPATAASLAGGTVVALAGAFVIGRSRDQGKN"
     gene            2269..6618
                     /gene="app"
                     /locus_tag="WJK66_001529"
     CDS             2269..6618
                     /gene="app"
                     /locus_tag="WJK66_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HEZ7579227.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGRNPDQHRFSYQIVKRNNYKPDNSHPYNGDYHM
                     PRLHKFVTDAEPVEMTSDMRGNTYSDKEKYPERVRIGSGHHYWRYDDDKHGDLSYSGA
                     WLIGGNTHMQGWGNNGVVSLSGDVRHANDYGPMPIAGAAGDSGSPMFIYDKTNNKWLL
                     NGVLQTGYPYSGRENGFQLIRKDWFYDDIYRGDTHTVFFEPRSNGHFSFTSNNNGTGT
                     VTETNEKVSNPKLKVQTVRLFDESLNETDKEPVYAAGGVNQYRPRLNNGENLSFIDYG
                     NGKLILSNNINQGAGGLYFEGDFTVSPENNETWQGAGVHISEDSTVTWKVNGVANDRL
                     SKIGKGTLHVQAKGENQGSISVGDGTVILDQQADDKGKKQAFSEIGLVSGRGTVQLNA
                     DNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNATTTSTVTITGNESI
                     TQPSGKNINRLNYSKEIAYNGWFGEKDTTKTNGRLNLVYQPAAEDRTLLLSGGTNLNG
                     NITQTNGKLFFSGRPTPHAYNHLGSGWSKMEGIPQGEIVWDNDWINRTFKAENFHIQG
                     GQAVISRNVAKVEGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCVEKTITDDKV
                     IASLTKTDISGNVSLADHAHLNLTGLATLNGNLSANGDTRYTVSHNATQNGNLSLVGN
                     AQATFNQATLNGNTSASGNASFNLSNNAAQNGSLTLSDNAKANVSHSALNGNVSLADK
                     AVFHFENSRFTGQLSGSKDTALHLKDSEWTLPSGTELGNLNLDNATITLNSAYRHDAA
                     GAQTGSVSDTPRRRSRRSLLSVTPPTSVESRFNTLTVNGKLNGQGTFRFMSELFGYRS
                     DKLKLAESSEGTYTLAVNNTGNEPVSLDQLTVVEGKDNKPLSENLNFTLQNEHVDAGA
                     WRYQLIRKDGEFRLHNPVKEQELSDKLGKAEAKKQAEKDNAQSLDALIAAGRDAAEKT
                     ESVAEPARQAGGENVGIMQAEEEKKRVQADKDSALAKQREAETRPATTAFPRARRARR
                     DLPQPQPQPQPQPQPQRDLISRYANSGLSEFSATLNSVFAVQDELDRVFAEDRRNAVW
                     TSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFSHNRTENTFDDGIGNS
                     ARLAHGAVFGQYGIGRFDIGISTGAGFSSGSLSDGIGGKIRRRVLHYGIQARYRAGFG
                     GFGIEPYIGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKADYSFKPAQHISITPY
                     LSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAAAAKGPQLE
                     AQHSAGIKLGYRW"
BASE COUNT         1792 a         1973 c         1678 g         1339 t
ORIGIN      
        1 aaacggatcg gattcccgtt tttatgggat aacggaatgt tcagccggac accgccacga
       61 tgctttcgat ccgtttattt tccgatgcag aatctcgaaa aaatcacgcc gagcaggtcg
      121 tccgccgtaa actcgcccgt gatttcgccg catgcgacct gagccaagcg caagtgttcg
      181 gcaaacagct cgatttgatg gttgccgcac aatgccgcca gcgacaattc ttcctgcgct
      241 gctttgagtg cgttgacgtg ccgcgtccgc gccaaaaaca gcccttcgct ttcgccctgc
      301 caaccggcct cacgcaacaa cgtccgtttc agcgcgtcca agccgtcgcc agttttcgcc
      361 gacagcgcga tgacggtttc cgcgcccgta ccgaacccgc ctgccgcgtg tgcgtgcaaa
      421 tcggatttgc tgtggatttc gatgcgtttc aactccggcg gcaacgcgtc caaaatcgcc
      481 cgtgtctttt cattcaaacc ctcgcgcgga tcgaccaaca ccagcgcgac atcggcttcg
      541 gatacggctt tgcggctgcg ttcgatgccg atgcgttcga ccacgtcgtc cgtctcgcgc
      601 aaacctgccg tatcgacaat atgcaccggc acgccgtcaa tcaggatacg ttccctgacc
      661 gcgtcgcgcg tcgttccggc aatatcggta acaatcgcca cttcgtcgcc cgccagcgcg
      721 ttcaacaggc tggatttgcc gacattcggc gcgccgacca atacgacatt cagaccttcg
      781 cgcaaaatcg cgccctgctg cgcgttggca agcacatcat ccacggcgcg gcgcaagccg
      841 tccagtttgc cgcgtgcgtc tgccgcttcg agaaaatcaa tgtcttcctc gggaaaatcc
      901 agcgtcgctt cgaccagcat ccgcaaggta atcaaatctt cgaccagacc gtgtatccgc
      961 cgcgaaaaat cgcccttgag cgagcgcaac gccagacgcg ccgccgaacg gctggatgcg
     1021 tcaatcaaat ccgccacgcc ttccgcctgt gccaagtcca gtttgtcgtt caaaaacgca
     1081 cgtttggtaa actcgcccgg ttcggcaagg cgcgcgccca attccaaaca gcggttcagc
     1141 agcatatcca tcaccaccgg cccgccgtgt ccctgaagct cgataacatc ttcgcccgta
     1201 aaacttgccg gtgcggcaaa aaacagcaaa agcccgctgt cgattgcctg accgtccgcg
     1261 tccgtaaaat cagcataggt tgcggtacgc ggtttgggcg ttttcccaca caaagcctct
     1321 gccatcggca gcaggttttt ccccgatatg cgtatcacgc ccacgccgcc gcgcccgggt
     1381 gcggtagcga ctgccgcaat cgttggaacg ttatccgaca taaaaccccc gaaaattcaa
     1441 aacagccgcg attatagcaa atgccgtctg aagtccgacg gtttggcttt cagacggtat
     1501 aaaaccgcaa aaatgcttga taaatccgtc cgcctgacct aatataacca tatggaaaaa
     1561 cgaaacacat acgccctcct gctcggtata ggctcgctgt tgggcctgtt ccatcccgca
     1621 aaaaccgcca tccgccccaa tcccgccgac gatctcaaaa acatcggcgg cgattttcaa
     1681 cgcgccatag agaaagcgcg caaatgaccg aaaacgcaca ggacaaggcg cggcaggctg
     1741 tcgaaaccgt cgtcaaatcc ccggagcttg tcgagcaaat cctgtccgac gagtacgtgc
     1801 aaataatgat agcccggcgt ttccattcgg gaccgttgcc gccgccgtcc gacttggcgc
     1861 aatacaacga cattatcagc aacggggcag accgcattat ggcaatggcg gaaaaagaac
     1921 aagccgtccg gcacgaaacc atacggcaag accaaacctt caacaggcgc gggcaactgt
     1981 acggcttcat cagcgtcatc ctgatactgc tttttgccgt cttcctcgta tggagcggct
     2041 accccgcaac cgccgcctcc cttgccggcg gcacagtggt tgccttggcg ggtgctttcg
     2101 tgattggaag aagccgagac caaggcaaaa attaatgaca aatcctaggg cgtgcttcat
     2161 atccgcccga acgccgaacc gcacatatag gcacatcccg cgcgccgccc ggaagcggaa
     2221 gccgcgccct cccaaacaaa cccgaatccc gtcagataag gaaaaataat gaaaacaacc
     2281 gacaaacgga caaccgaaac acaccgcaaa gccccgaaaa ccggccgcat ccgcttctcg
     2341 cctgcttact tagccatatg cctgtcgttc ggcattcttc cccaagcctg ggcgggacac
     2401 acttatttcg gcatcaacta ccaatactat cgcgactttg ccgaaaataa aggcaagttt
     2461 gcagtcgggg cgaaagatat tgaggtttac aacaaaaaag gggagttggt cggcaaatca
     2521 atgacaaaag ccccgatgat tgatttttct gtggtgtcgc gtaacggcgt ggcggcattg
     2581 gtgggcgatc aatatattgt gagcgtggca cataacggcg gctataacaa cgttgatttt
     2641 ggtgcggaag gaagaaatcc cgatcagcac cgtttttctt accaaattgt gaaaagaaat
     2701 aattataagc ctgacaattc acacccttac aacggcgatt accatatgcc gcgtttgcat
     2761 aaatttgtca cagatgcaga acctgtcgaa atgacgagtg acatgagggg gaatacctat
     2821 tccgataaag aaaaatatcc cgagcgtgtc cgcatcggct caggacacca ctattggcgt
     2881 tatgatgatg acaaacacgg cgatttatcc tactccggcg catggttaat tggcggcaat
     2941 acacatatgc agggttgggg aaataatggc gtagttagtt tgagcggcga tgtgcgccat
     3001 gccaacgact atggccctat gccgattgca ggtgcggcag gcgacagcgg ttcgccaatg
     3061 tttatttatg acaaaacaaa caataaatgg ctgctcaacg gagttttaca aaccggctac
     3121 ccttattccg gcagggaaaa cggtttccag ctgatacgca aagattggtt ctacgatgac
     3181 atttacagag gcgatacaca taccgtcttt tttgaaccgc gcagtaacgg acatttttcc
     3241 tttacatcca acaacaacgg tacgggtacg gtaacagaaa ccaacgaaaa ggtttccaat
     3301 ccaaagctta aagtacagac agtccgactg tttgacgaat ctttgaatga aactgataaa
     3361 gaaccagttt acgcggcagg gggtgttaat cagtaccgtc caaggttaaa caacggtgaa
     3421 aacctttctt ttatcgatta cggcaacggc aaactcatct tatcaaacaa catcaaccaa
     3481 ggcgcgggcg gtttgtattt tgaaggtgat tttacggtct cgcctgaaaa caacgaaacg
     3541 tggcaaggcg cgggcgttca tatcagtgaa gacagtaccg ttacttggaa agtaaacggc
     3601 gtggcaaacg accgcctgtc caaaatcggc aaaggcacgc tgcacgttca agccaaaggg
     3661 gaaaaccaag gctcgatcag cgtgggcgac ggtacagtca ttttggatca gcaggcagac
     3721 gataaaggca aaaaacaagc ctttagtgaa atcggcttgg tcagcggcag gggtacggtg
     3781 caactgaatg ccgataatca gttcaacccc gacaaactct atttcggctt tcgcggcgga
     3841 cgtttggatt taaacgggca ttcgctttcg ttccaccgta ttcaaaatac cgatgaaggg
     3901 gcgatgattg tcaatcataa tgccacaaca acatccaccg ttaccattac agggaatgaa
     3961 agtattacac aaccgagtgg taagaatatc aatagactta attacagcaa agaaattgcc
     4021 tacaacggtt ggtttggcga gaaagatacg accaaaacga acgggcggct caaccttgtt
     4081 taccagcccg ccgcagaaga ccgcaccctg ctgctttccg gcggaacaaa tttaaacggc
     4141 aacatcacgc aaacaaacgg caaactgttt ttcagcggca gaccgacacc gcacgcctac
     4201 aatcatttag gaagcgggtg gtcaaaaatg gaaggtatcc cacaaggaga aatcgtgtgg
     4261 gacaacgact ggatcaaccg cacgtttaaa gcggaaaatt tccatattca gggcgggcag
     4321 gcggtgattt cccgcaatgt tgccaaagtg gaaggcgatt ggcatttgag caatcacgcc
     4381 caagcagttt ttggtgtcgc accgcatcaa agccatacaa tctgtacacg ttcggactgg
     4441 acgggtctga caaattgtgt cgaaaaaacc attaccgacg ataaagtgat tgcttcattg
     4501 actaagaccg acatcagcgg caatgtcagc cttgccgatc acgctcattt aaatctcaca
     4561 gggcttgcca cactcaacgg caatcttagt gcaaatggcg atacacgtta tacagtcagc
     4621 cacaacgcca cccaaaacgg caaccttagc ctcgtgggca atgcccaagc aacatttaat
     4681 caagccacat taaacggcaa cacatcggct tcgggcaatg cttcatttaa tctaagcaac
     4741 aacgccgcac aaaacggcag tctgacgctt tccgacaacg ctaaggcaaa cgtaagccat
     4801 tccgcactca acggcaatgt ctccctagcc gataaggcag tattccattt tgaaaacagc
     4861 cgctttaccg gacaactcag cggcagcaag gatacagcat tacacttaaa agacagcgaa
     4921 tggacgctgc cgtcaggcac ggaattaggc aatttaaacc ttgacaacgc caccattaca
     4981 ctcaattccg cctatcgcca cgatgctgca ggcgcgcaaa ccggcagtgt gtcagacacg
     5041 ccgcgccgcc gttcgcgccg ttccctatta tccgttacac cgccaacttc ggtagaatcc
     5101 cgtttcaaca cgctgacggt aaacggcaaa ttgaacggtc aaggaacatt ccgctttatg
     5161 tcggaactct tcggctaccg aagcgacaaa ttgaagctgg cggaaagttc cgaaggcact
     5221 tacaccttgg cggtcaacaa taccggcaac gaacccgtaa gcctcgatca attgacggta
     5281 gtggaaggga aagacaacaa accgctgtcc gaaaacctta atttcaccct gcaaaacgaa
     5341 cacgtcgatg ccggcgcgtg gcgttaccaa ctcatccgca aagacggcga gttccgcctg
     5401 cataatccgg tcaaagaaca agagctttcc gacaaactcg gcaaggcaga agccaaaaaa
     5461 caggcggaaa aagacaacgc gcaaagcctt gacgcgctga ttgcggccgg gcgcgatgcc
     5521 gccgaaaaga cagaaagcgt tgccgaaccg gcccggcagg caggcgggga aaatgtcggc
     5581 attatgcagg cggaggaaga gaaaaaacgg gtgcaggcgg ataaagacag cgccttggcg
     5641 aaacagcgcg aagcggaaac ccggccggct accaccgcct tcccccgcgc ccgccgcgcc
     5701 cgccgggatt tgccgcaacc gcagccccaa ccgcaacctc aaccccaacc gcagcgcgac
     5761 ctgatcagcc gttatgccaa tagcggtttg agtgaatttt ccgccacgct caacagcgtt
     5821 ttcgccgtac aggacgaatt ggaccgcgtg tttgccgaag accgccgcaa cgccgtttgg
     5881 acaagcggca tccgggacac caaacactac cgttcgcaag atttccgcgc ctaccgccaa
     5941 caaaccgacc tgcgccaaat cggtatgcag aaaaacctcg gcagcgggcg cgtcggcatc
     6001 ctgttttcgc acaaccggac cgaaaacacc ttcgacgacg gcatcggcaa ctcggcacgg
     6061 cttgcccacg gcgccgtttt cgggcaatac ggcatcggca ggttcgacat cggcatcagc
     6121 acgggcgcgg gttttagcag cggcagtctt tcagacggca tcggaggcaa aatccgccgc
     6181 cgcgtgctgc attacggcat tcaggcacga taccgcgccg gtttcggcgg attcggcatc
     6241 gaaccgtaca tcggcgcaac gcgctatttc gtccaaaaag cggattaccg ctacgaaaac
     6301 gtcaatatcg ccacccccgg tcttgcgttc aaccgctacc gcgcgggcat taaggcagat
     6361 tattcattca aaccggcgca acacatttcc atcacgcctt atttgagcct gtcctatacc
     6421 gatgccgctt cgggcaaagt ccgaacacgc gtcaataccg ccgtattggc tcaggatttc
     6481 ggcaaaaccc gcagtgcgga atggggcgta aacgccgaaa tcaaaggttt cacgctgtcc
     6541 ctccacgctg ccgccgccaa aggcccgcaa ctggaagcgc aacacagcgc gggcatcaaa
     6601 ttaggctacc gctggtaacc gccggatatg ccgaaagggg tctgacgatg ccaccgtgcg
     6661 ctgtcaaacc ctttttctgc cgccgccttg tgcctgctcc attgtctgat aaccgtcaaa
     6721 ttgattttta gcccatgttt ggtgggtcgg ggaaatctat atcttcgtcc gtgccgaaat
     6781 ag
//
LOCUS       DAWKOG010000106         6756 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.106, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000106 DAWKOG010000000
VERSION     DAWKOG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6756
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.106"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(110..469)
                     /gene="rplT"
                     /locus_tag="WJK66_001530"
     CDS             complement(110..469)
                     /gene="rplT"
                     /locus_tag="WJK66_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEZ7579228.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(482..679)
                     /gene="rpmI"
                     /locus_tag="WJK66_001531"
     CDS             complement(482..679)
                     /gene="rpmI"
                     /locus_tag="WJK66_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEZ7579229.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(826..1347)
                     /gene="infC"
                     /locus_tag="WJK66_001532"
     CDS             complement(826..1347)
                     /gene="infC"
                     /locus_tag="WJK66_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEZ7579230.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(1365..3278)
                     /gene="thrS"
                     /locus_tag="WJK66_001533"
     CDS             complement(1365..3278)
                     /gene="thrS"
                     /locus_tag="WJK66_001533"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEZ7579231.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGAGLAKAAVAGKVNGKL
                     VDACDPIVEDSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVKLSLRPEKRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVKDALGRSWQCGTLQLDFVLPERLNAEYVTENNDRARPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQLVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(3331..3407)
                     /locus_tag="WJK66_001534"
     tRNA            complement(3331..3407)
                     /locus_tag="WJK66_001534"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3371..3373),aa:Val,seq:gac)
     gene            3586..4701
                     /gene="tgt"
                     /locus_tag="WJK66_001535"
     CDS             3586..4701
                     /gene="tgt"
                     /locus_tag="WJK66_001535"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEZ7579232.1"
                     /translation="MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQARKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLKGLEELDFPGLAVGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGVDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMREAVEQGKFADWQAQFHENRARGTD"
     gene            4711..5793
                     /locus_tag="WJK66_001536"
     CDS             4711..5793
                     /locus_tag="WJK66_001536"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEZ7579233.1"
                     /translation="MPSEAASDGILPRYPTRTIFQKPIMLPFLPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRVVELPKWLWYPILHGLVLTLRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAKRMPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLV
                     IPMYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHHDLGDPYPDECRHTAKLLAEALELTEDQYVVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKSYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            5984..6385
                     /locus_tag="WJK66_001537"
     CDS             5984..6385
                     /locus_tag="WJK66_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ7579234.1"
                     /translation="MNTTRLPTALVLGCLCAAASAADNSIMTKGQKVYESNCVACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKGKKN"
     gene            complement(6448..6738)
                     /locus_tag="WJK66_001538"
     CDS             complement(6448..6738)
                     /locus_tag="WJK66_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579235.1"
                     /translation="MSKYFTETDIGVREHFDFFGEFVVSPAARSDDLALAYGLKLEAG
                     EEGLSLAELFDKRSDSQEPVECGRIDIGGFMLTAKEVDGDGNIGSMGLKVPR"
BASE COUNT         1736 a         1829 c         1562 g         1629 t
ORIGIN      
        1 aacgcaaatt tatttaaaaa aatagacttt aaagaaagaa aatcaatcac atagaatttc
       61 tgcaaacaaa gaaaaaagga agccgcagct tccttttcaa ttttttggat taagcagcca
      121 aagcagcttt ggctttttca accaattgtg caaaagcggc tttatcgaac acggccaaat
      181 cagccaatac tttgcggtcg atttcaatag aggcgcgttt cagaccgttc ataaatttgc
      241 tgtaagacaa cccgttttca cgcgcacctg cattgatacg gacaatccac aattgacgga
      301 attggcgttt gcgttggcgg cggtcacggt acgcgtattg accggctttc attaccgcct
      361 gcttggcaac gcggtaaacg tttttacgac ggccgcggta gcctttggct aacgcgaaga
      421 ttttttggtg acgggcacga gcggtaacac cgcgttttac gcgtggcata ttctaaactc
      481 cttaagcgta gggtaacatt ttagcaacag aagccaaatc gcgatcattt accatagagg
      541 taccgcgcag ttggcgtttg tttttggtgg tctttttagt caagatgtgg cgtttgaacg
      601 catgagcgcg tttcacaccg ccgttaccca gtactttaaa gcgttttttc gcgctagact
      661 tggttttcat tttaggcatg ggaaaactcc attcgttatc ggataaggca ttagggggtt
      721 ttaaaacatc agtttcaaac acttgaaccc acaataccac ggttttttgc cgcaattgaa
      781 aacttactcc gaagcggcaa tcggagtaag cggagaatta tagctttatt ttttcttcgg
      841 cgcaatcatc atcaccattt ggcgaccttc cattttggga aaggactcga tttgcgccac
      901 ttcagccaaa tcttctttta cacgttccaa aagttgcgcg ccgagttgct ggtgagccat
      961 ttcacggccg cggaaacgca atgtcacttt gactttatcg ccgtcggcaa ggaagcggtt
     1021 aatgttgcgc atcttgattt gataatcgcc ctcatccgta cccggacgga atttgatttc
     1081 cttgatttgc acctgctttt ggtttttctt ggcttcgtcg cgtttcttgg cctgctggta
     1141 tttgtattta ccgtaatcca tcagtttgca cacaggcggt ttagcagttg gggaaatctc
     1201 taccaaatcg acatcctgcc cttcggccat agccaaagct tcacgaactg aaacgacacc
     1261 aagctgttcg cctgactcac tgattaaacg cacttctttg gcggtaattt cgccgttgat
     1321 tcgtgcttcg cgttcttgag cgatgatgaa tactcctata aaaattaatg attgacgagg
     1381 gcatcagtga tttcttgctg caattgcgca atgaaatcat ccaaatccaa agaacccaaa
     1441 tcttctgctt tgcggcgtac cgccactttg ttttcctgct tctccttatc gccgacaacg
     1501 atttgataag ggaaacggta ttggctgttg tcgcggattt tgtaaccgat tttttcgtta
     1561 cgcaaatcca actcggcgcg gaatcctgcc gcctgcaatt tggcagccac ttcccgacaa
     1621 taatctgcct gattttcggt aatattcata attaccaatt gaaccggagc caaccataac
     1681 gggaatgagc ctgcatggtt ctcaatcaga atgccgataa accgctccaa agaacctaaa
     1741 atggcgcgat gcaacataac aggacgcgca cggtcgttgt tttcagttac atattcggca
     1801 ttcaaacgct ccggcaatac gaaatccagt tgtaatgtac cgcattgcca agaacgaccc
     1861 aaggcatctt tgacatgata ttcgatttta ggcccgtaaa acgcaccctc gcccggcaat
     1921 tcgccccatt ccacgccgca ggcagtcaat gcctcgcgca aaccctgctc tgccttatcc
     1981 cacacgtcat ctgaacctgc acgtttttca gggcgaagag aaagcttgac ggatacatca
     2041 tggaaaccga actgtttgta gatgcgaacc aacaattcat tgaacgcacg agcctcgctg
     2101 acgatttgat cttcggtaca gaaaatatgc gcatcatcct gcacaaaacc gcgaacccgc
     2161 atcagaccgt gcagcgcacc gctcggctca ttgcggtggc aagaaccgaa ttccgccaaa
     2221 cgcatcggca aatctcgata cgaacgcaaa ccgttgttaa aaatttgaac atgacccgga
     2281 cagttcatcg gtttaaccgc atattcgcgt ttttccgaac tggttacgaa catattgtct
     2341 ttgtagttgt cccaatggcc ggatttttcc caaaaggttt tatccatgat ttgaggcgtt
     2401 ttgacctctt tataaccggc ggcattcagc tctttccgca tatgctgctc aatcacttgc
     2461 cacaaagccc agcctttggg atgccaaaac accatacccg gtgcttcgtc ttgcaagtgg
     2521 aacaaatcca gttgtttgcc aagcttgcgg tggtcgcgct tttcggcttc ttcgatacgt
     2581 tgaatatagg ctttcaactc gtcttttgtc gcccaagccg taccgtagat gcgttgcagc
     2641 atttcattgt tgctgtcgcc gcgccagtat gcgcccgcca acttggtcag tttgaagttt
     2701 ttcaggaaac gggtattcgg aacgtgcggg ccgcggcaca tatcgacata ttcctggtga
     2761 tgatacatcc ccatcgcttc cacttcgggc atatcgtcaa tcaggcgcag tttgtattct
     2821 tcgccgcgct cttgaaaaat tttaatcgcc tccgcacgcg gagtcatgat tttgaccaca
     2881 tcatagtctt gggcaatcaa ttctttcata cgcgcttcaa tggcggcaac atcttccggt
     2941 gtaaacggtt tttccgtggc gatgtcgtaa taaaagccct cttcaatgac ggggccgata
     3001 accatttttg cattaggata gagttgcttg acggcatgcc cgacaagatg cgcgcaggaa
     3061 tggcggatga tttcgatgcc ttcctgatct ttcggagtaa tgatttgaac agcagaatct
     3121 tcaacaatcg ggtcgcacgc atcgaccaat ttgccgttta ccttgcctgc caccgccgcc
     3181 ttcgccaaac cggcaccgat agacgcagca atttgagcca cggtaacggg ggattcgtat
     3241 tggcggactg agccgtccgg caaggtaata ttcaacatca aacgctccga aagattaaaa
     3301 aataacggca taaatgccgt tatggggatt tggtaggcac gattggattc gaaccaacga
     3361 cccccaccat gtcaaggtgg tgctctaacc aactgagcta cgtgccttca aagaattttc
     3421 gcattttatc gagtcgtttt aatttttgca agaggtatat gcatttcttt atgcaacatt
     3481 gtggcaagat ttggggatgt cgtctgaaaa accgtgggaa ttcatttata atgccgtctt
     3541 ttaaaacata tttcgacccc tgtcgcccaa ctgacattca atcttatgct taaatttact
     3601 ttacacaaaa aagacggata cgcccgacgc ggcacgctgg agttgaacca cggcaaaatt
     3661 gaaacgccgg tatttatgcc tgtcggtact tacggttcgg ttaaggcgat gaacccgcaa
     3721 aacctgcacg acatcaaggc gcaaattatt ttgggcaaca cttatcattt gtggctgcgt
     3781 ccgggcttgg aggtcgtcga acagtttgga gggcttcacg gctttatcgg ctgggacaaa
     3841 ccgattttga ccgactcggg cggttttcag gttttttctt tgtcggatat gcgcaagctg
     3901 acggaagaag gctgtacgtt caaaagcccg atcaacggcg acaagctgtt tttatcgcct
     3961 gaaatttcca tgaaaatcca aacggttttg aactcggaca tcgcgatgca gctggacgaa
     4021 tgcacgccgg gcgaagcaac gcgcgaacag gcgcgaaaat cgctgcaaat gagcctgcgc
     4081 tgggcggaac ggagcaaaaa agcctttgaa gatttgaaaa acccgaacgc actgttcggc
     4141 atcgtgcaag gcgcgatgta tgaagatttg cgcgaagagt cgctgaaagg cttggaagag
     4201 ctcgatttcc ccggcttggc cgtcggcggc ttgtccgttg gcgaacctaa gcccgaaatg
     4261 taccgcatgt tgcgcgccgt cggccctatc ctgccggaac acaaacctca ttatttaatg
     4321 ggcgtcggta cgcctgaaga cttagtgtac ggcgtggcac acggcgtgga tatgttcgac
     4381 tgcgtcatgc ctactcgcaa tgcgcgtaac ggctggctgt ttacccgttt tggcgatttg
     4441 aaaatcaaaa acgccaaaca caagctcgac aagcgtccga tagacgaaag ctgcacctgc
     4501 tacgcctgcc aaaacttcag ccgcgcctac ctgcaccatc tgcaccgcgc cggcgaaatc
     4561 ttgggcgcac agttgaacac catccacaat ctgcatttct accaagtcat tatggcggaa
     4621 atgcgcgaag ccgtcgaaca aggcaaattt gccgactggc aagcacaatt ccacgaaaac
     4681 cgcgcacgcg gcacagactg actccctgca atgccgtctg aagccgcttc agacggcatt
     4741 ttgccgcgtt accctacccg cactattttt cagaaaccga tcatgctccc attccttcca
     4801 gaaccctccc tttcctatac gcaacaaaac cgtaccgccg ttttgctgtt gaacctcggc
     4861 acgcccgacg cgccgaccgc gcaggcggta cgaccttatc tgaagtcctt tctgaccgac
     4921 cggcgcgtcg tcgaactgcc caaatggctg tggtatccca ttctgcatgg tctggtactg
     4981 acgctccgcc ccaaaaaaag cgcgcacgcc tatgaaaaaa tctggtttaa ggaaggttcg
     5041 cccttagaag tttacacagc acgccaagct gccgcgcttg ccaaacgtat gcccgacctc
     5101 atcgtccgcc acgccatgac ctacggcaat ccttcggtgg cagacgtatt gtcggaactg
     5161 aaggcgcaag gcgcgggcag actgctggtc atcccgatgt acccgcaata tgccgcttcc
     5221 agcagcggag cggcagtaga taaggtgtgc gaacaattac tgctgcaacg gaaccaaatg
     5281 agcgtccgca ccgtttcgcg cttttatgac gataccggct acattgatgc gatgaaaaac
     5341 cacatcctcc gatattgggc ggaacacgga cgtgggaaaa aactgatgtt gagttttcac
     5401 ggcgtaccgc agaagcacca cgacctcggc gacccctatc ccgacgagtg ccgccacacc
     5461 gccaaactgc ttgccgaagc actggaactg accgaagacc aatatgtcgt atcgttccaa
     5521 agccaattcg gcagggcaaa atgggttaca ccgagcacgc aggatttgtt cggcaaactg
     5581 cccaaacaag gcgtaaccga actggacgta ttctgcccgg gctttttggc agactgtttg
     5641 gaaaccatgg aagaaatcgc cctgatgggg cgtgaacagt tttacgaagc aggcggcaaa
     5701 agctaccgct acatcccctg cctcaacgac aaccccgact ggatagacgc actcgtcgca
     5761 cttgccgaag aaaaccttgg cggctggcgt tgaaactgtt tgacccatgt ctaaaatgcc
     5821 gtctgaacgc cgtgttcaga cggcattttc ccgctacttt agcgtatgat ggcatctatg
     5881 ctggacagca catcaagtgc aaccttacca ccgattcggc aaaaaaacct ttcacccgct
     5941 acgcaaaatc aacaacaagg tttcaaacat taaggaaaca ccaatgaaca caacccgact
     6001 gccgaccgcc ctcgtcttgg gctgcctctg cgccgccgct tctgccgccg acaacagcat
     6061 catgacaaaa ggacaaaaag tgtacgaatc caactgcgtc gcctgccacg gcaaaaaggg
     6121 cgaaggtcgc ggaaccatgt ttccgccgct ctaccgctcc gacttcatca tgaaaaaacc
     6181 gcaggtgctg ctgcacagca tggtcaaagg catcaacggt acaatcaaag tcaacggcaa
     6241 aacctacaac ggattcatgc ccgcaaccgc catcagcgat gcggacattg ccgccgtcgc
     6301 cacttatatc atgaacgcct ttgacaacgg cggcggaagc gttaccgaaa aagacgtaaa
     6361 acaggcaaaa ggcaaaaaaa actaaacaga caaaatgccg tctgaaaccg gcaatccggc
     6421 ttcagacggc attcaaatca aacctttcta acgcggtact ttcagcccca tagacccgat
     6481 attgccgtcc ccgtcaacct ccttcgcggt cagcataaag ccgccgatgt caatacgacc
     6541 gcactcgacc ggctcctgac tgtcggaacg cttatcgaaa agctcggcaa ggctcaaacc
     6601 ctcttcgccc gcttccagct tcaaaccgta agcaagtgcc aaatcgtccg aacgtgctgc
     6661 cggcgaaacg acaaactcgc cgaagaaatc aaaatgctca cggacaccga tatccgtctc
     6721 ggtaaagtat ttcgacattt tatagtgaac taaatt
//
LOCUS       DAWKOG010000107         6730 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.107, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000107 DAWKOG010000000
VERSION     DAWKOG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.107"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(239..1045)
                     /locus_tag="WJK66_001539"
     CDS             complement(239..1045)
                     /locus_tag="WJK66_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ7579236.1"
                     /translation="MQNHVISLASAAERRAHIADTFGRHDIPFQFFDALMPSEALEQA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYIAVFEDDVLLGEGAEKFLA
                     EDAWLQERFDKDSAFIVRLETMFMHVLTSPSGVADYGGRAFPLLESEHFGMAGYIISR
                     KAMRFFLDRFAVLPSERLKAVDWMLFSSFLDKGGMTVCQLTPALCVQSETLPSQLKNG
                     RQESYRNRRSPKVLLKRALGKIGREIERARERKRQKKLEKHLGRHVVPFE"
     gene            complement(1087..1182)
                     /locus_tag="WJK66_001540"
     CDS             complement(1087..1182)
                     /locus_tag="WJK66_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lacto-N-neotetraose biosynthesis glycosyl
                     transferase"
                     /protein_id="HEZ7579237.1"
                     /translation="MDIVFAADGRMRRLFTLRQYFGILHRLLKNR"
     gene            complement(1194..2033)
                     /locus_tag="WJK66_001541"
     CDS             complement(1194..2033)
                     /locus_tag="WJK66_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ7579238.1"
                     /translation="MQNHVISLASAAERRAHIAATFGARGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYIAVFEDDVLLGEGAEKFLA
                     EDAWLQERFDKDSAFIVRLETMFMHVLTSPSGVADYGGRAFPLLESEHWGTAGYIISR
                     KAMRFFLDRFAALPPEGLHPVDWMMFSDFFDREGMPVCQLDPALCAQELHYAKFHDQN
                     SALGSLIEHDRLLNRKQQRRDSPANTFKHRLIRALTKISREREKRRQRREQLIGKIIV
                     PFQ"
     gene            complement(2075..3119)
                     /locus_tag="WJK66_001542"
                     /pseudo
     CDS             complement(2075..3119)
                     /locus_tag="WJK66_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779608.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            complement(3136..5199)
                     /locus_tag="WJK66_001543"
     CDS             complement(3136..5199)
                     /locus_tag="WJK66_001543"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEZ7579239.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAVKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGGLLEDIINQVVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDIVNVSVLGLQSSNQTLGHRFLSNGEITIENADSYAEQMRGQGKVVA
                     SFAERKAAIQTALEGQARSLNAAVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLSSVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAVEQHYQPRFAGDKLPESKIA
                     TAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAELGEVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            complement(5220..5630)
                     /locus_tag="WJK66_001544"
     CDS             complement(5220..5630)
                     /locus_tag="WJK66_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HEZ7579240.1"
                     /translation="MPVYFRTPSRKRLHKMNRRQHKKLHLGEFQALIFGVRGSLHQAA
                     DDAEAFDRFMDELIAYLEANGLEMAGGGDCGDFSCLFQKSGARSLGETEKKAIISWLA
                     GREDVATLYATPLVDGFYGDDGWENYSETYKQAD"
     gene            complement(5706..6611)
                     /gene="glyQ"
                     /locus_tag="WJK66_001545"
     CDS             complement(5706..6611)
                     /gene="glyQ"
                     /locus_tag="WJK66_001545"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEZ7579241.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTPDGNTVSYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAEENAALALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
BASE COUNT         1418 a         1774 c         1857 g         1681 t
ORIGIN      
        1 caggcaggaa tctagaattt taatgcggca agaatttatc ggaaaaaacc gaagtttaaa
       61 gacctagatt cccgcctgcg cgagaatgac gagattttag gtttctgttt ttggctttct
      121 gttttctcgg gaatcacgaa ttagggctta cccgaaaaca agtgaaaccg aacaaaccgg
      181 attccccgac ataaatttag ggacagcgca cacccgccaa ataaggaaag gccaaaacct
      241 attcaaacgg gacaacgtgc cgcccgaggt gtttttccaa ttttttctga cgtttccgtt
      301 ccctcgccct ttcgatttcc ctgccgattt ttcccaaagc gcgtttcaac aaaaccttcg
      361 gggagcggcg gttgcggtac gattcctgcc tgccgttttt cagttggctg ggcagggttt
      421 cgctttggac gcacaaggcg ggggtcagtt ggcaaaccgt catcccccct ttgtcgagaa
      481 aactgctgaa gagcatccaa tccaccgcct tcaggcgttc agacggcaaa acggcaaacc
      541 tgtccaaaaa gaaacgcatc gcctttcggg aaataatata gcccgccata ccaaaatgtt
      601 cgctttccaa cagcggaaag gcgcgcccgc cgtagtccgc cacgccggag ggcgaggtca
      661 ggacgtgcat aaacatcgtt tccaagcgga cgataaaggc ggaatccttg tcaaaacgct
      721 cttgcagcca agcgtcttcg gcaaggaatt tctccgcacc ttcgccgagt aaaacgtcgt
      781 cctcaaatac ggcgatatac ggcagacctt catccaatgc ctgcttccac aatacggcgt
      841 ggctcataaa gcaggctttt tccactccgc tcaaataggg gtgcgccgac aaaccgggta
      901 cgagttccgc cattgcctgc tccagtgctt cagacggcat cagtgcgtcg aaaaactgaa
      961 acggaatgtc gtgcctgccg aaggtatcgg caatgtgcgc cctgcgttct gcagcggaag
     1021 ccaagctgat aacgtggttt tgcataattt atcctgtttt ttgtctgttg gataaagcgg
     1081 cgtttttcaa cggtttttca gcaatcggtg caaaatgccg aagtattgcc tcaaggtaaa
     1141 cagccgccgc atcctgccgt ctgccgcaaa tacgatgtcc atcttttctc cttttattgg
     1201 aaaggcacaa tgatcttgcc gattaactgt tcgcgccttt gccgacgttt ttccctttcc
     1261 ctgctgattt tggtcaaggc gcggatcagg cggtgtttga atgtgttggc gggggaatcg
     1321 cgcctttgct gtttgcggtt caggaggcgg tcgtgttcga tcaggctgcc caatgcgctg
     1381 ttttggtcgt gaaacttggc ataatgcagc tcttgggcgc acaaggcggg atcgagctgg
     1441 caaaccggca ttccttccct gtcgaaaaaa tcgctgaaca tcatccaatc gacggggtgc
     1501 agcccttcgg gcggcagggc ggcaaacctg tccaggaaaa accgcatcgc ttttcgggaa
     1561 atgatatagc ccgccgtccc ccagtgttcg ctttccaaca gcgggaaggc gcgcccgccg
     1621 tagtccgcca cgccggaggg cgaggtcagg acgtgcataa acatcgtttc caagcggacg
     1681 ataaaggcgg aatccttgtc aaaacgctct tgcagccaag cgtcttcggc aaggaatttc
     1741 tccgcgcctt cgccgagtaa aacgtcgtcc tcaaatacgg cgatatacgg cagaccttcg
     1801 tccaatgcct gtttccacaa tacggcgtgg ctcataaagc aggctttttc cactccgctc
     1861 aaataggggt gcgccgacaa gccggggacg agttccgcca ttgcctgttc cagcctttca
     1921 gacggcatca gtgcgtcgaa aaactgaaac gggatgccgc gcgcgccgaa ggttgcggca
     1981 atgtgcgccc tgcgttctgc ggcggaagct aagctgataa cgtggttttg cataatttat
     2041 cctgtttttt gtctgttgga taaagcggcg tttttcaacg gtttttcagc aatcggtgca
     2101 aaatgccgaa gtattgcctc aaggtaaaca gccgccgcat cctgccgtct gccgcaaaat
     2161 ccagccacgc gccggcgggc agcgtgtccg tccgtttgaa gcattggtac aaaaaccggc
     2221 gggcgcgttc aaaatcttct tccggcaaat gtttctccag caattcatac gctactgctt
     2281 ttatttggcg gtattcaagg ctgtcgaacc gggttttaaa acccatagac tgcaaaaaat
     2341 cgtttctggc ggttttttgg atgccctgcg cgatttcgtg ttggcggacg ctgtatttgg
     2401 atgaaacctg attggcgtga aggcggtatt tgaccaaggc ttcgggataa taagccagcc
     2461 tgcccaattt actgacatcg taccaaaatt ggtaatcttc cgcccaatcc cgctcggtgt
     2521 tgtaacgcaa accgccgtca atgacgctgc gccgcataat catcgtgttg ttgtgtatgg
     2581 ggttgccgaa agggaaaaag tcggcaatat cttcgtgccg ggtcggcttt ttccaaattt
     2641 tgccgtgcct gtgatgccgc gccagccggt tgccgtcctt ttcttccgac aacacttcca
     2701 gccacgcgcc catcgcgatg atgctgcggt ctttttccat ctcgcccacg attttctcaa
     2761 tccagtcggg ggcggcaata tcgtcggcat cggtgcgtgc aatatattcc cccccccgac
     2821 tttgccaatt cgtccagccc gatgtttaaa gagggaatca ggccggaatt ttgagcttgt
     2881 gcaaggattt tgatacggct gtcccgcttt tgaaaatcct tggcaatggc aagcgtaccg
     2941 tctgtcgagc cgtcatcgac aatcaaaata tccaagttgc accaagtttg attcacgacg
     3001 gcggctaatg attgggcgaa atatttttct acgttgtagg cgcaaatcaa tacgctgact
     3061 aaaggctgca atttattctc ccgataggca cgatgccgtc tgaaggcttc agacggcatt
     3121 tggactgtac aacggttact cgcccaaaag cgcgatgtcg gctaccgcgt tcatttgctc
     3181 tgccaagcgg ttcagcaggt tcaggcggtt ttgttttacg gcggcatctt ccgccattac
     3241 catcacgccg tcaaagaatg catcgacttg cggtttgacg gaagccagtt cggacaaggc
     3301 ggtttggaaa ttgccttcgg cgacggcggc ggcgattttc ggctgcaagc cttgcgcggc
     3361 ggcaaagagg gctttttctt cgtcctgttg cagcaggctt tcattgactt cgcccaactc
     3421 ggcatcggct tttttcagca ggttttgcac gcgtttgttg gcggcggcga gcgcggcggc
     3481 ttcgggcagc tgtttgaacg cggcaacggc ttgcagtttg gcggtcaaat cgtccaaacg
     3541 gcgcggctgt ttcgccaata cggcggcaac gatgtcttgc ggataatcgt tttgcagcaa
     3601 cacggccaaa cgcgcctgca taaagtcggc ggtttcagac ggcgtttttt cgttgagcaa
     3661 acctttgggg aagctgtcga atgccgtctg aatcagttcg ttcacgtcca aaccatactg
     3721 catcagcata cgcaaaatac ccaaggcagc gcggcgcagg gcgtaggggt ctttgtcgcc
     3781 ggtcggaatc agaccgatgc cccaaatgcc gaccagggtt tccagcttgt cggcaagtgc
     3841 aacggcagtg gcaattttgc tttcgggcag cttgtcgccg gcaaaacgcg gttggtaatg
     3901 ttgctcgacg gcttcggcga tttcttcggt ttcgccgtcc aagcgggcat agtatttgcc
     3961 catcgtgcct tgcagttcgg gaaactcgcc gaccatttcg gttaccaagt cggctttggc
     4021 aagacgcgcg gcgcgttcgg cagcggcggc atccgcgccc aatgctttgg cgatgtgggc
     4081 ggcaatgctt tgcaggcgtt cgatgcgttc ggcttgcgag ccgattttgt tgtggtacac
     4141 cacgctggac aatttcggca ggcggctttc caaagtcgct ttttggtctt gtttgtagaa
     4201 gaactcggca tcagacaggc gcgcgcgcaa aacgcgttcg ttgcctcgga tgatgtgcga
     4261 cgggtcttcg gtttgcaggt tggaaaccag caggaagcgg ttcatcagct tgccgttttg
     4321 gtcgagcagc ggaaagtatt tttggttttg ctgcatcgtt aaaatcaagc attcctgcgg
     4381 cacggcgagg aaatgttcct caaaaccggc ttccaaaaca acaggccatt cgaccagcgc
     4441 ggttacttcg tccaacaatg cttcatcggc ggcgacggcg gcgttcagac tgcgcgcctg
     4501 cccttccaat gccgtctgaa tcgcggcttt gcgctcggca aacgaagcga cgactttgcc
     4561 ttgcccgcgc atttgttcgg cgtagctgtc ggcgttttca atggtgattt cgccgttgga
     4621 caagaagcgg tgtcccaagg tttgattgct gctttgcaga cccaaaacgc tgacgtttac
     4681 gatgtcgccg ccgtgcagca caatcagccc gtgaacaggg cgcacgaagg taaatgtgct
     4741 gctgccccaa cgcatcactt tgggaatcgg cagtttctta accacttgat tgataatgtc
     4801 ttccaaaagt ccgcccagcg gcttgccggt ttggacgtat tcgtaggcgt acacatcctg
     4861 cttgccgtcg tggacgatgg tcaagtcttc gattttcgcg cctgcaccgc gtgcgaaacc
     4921 ttccaaagcc ttggtcggcg caccgtcttt tacggcattc gccacggcag ggcctttttt
     4981 cacgattttt tggtcggcct gaacggcttt gacgtttttg acttgaaccg ccaaacggcg
     5041 cggcgaggca taagccgtaa attcggctgc accgtcaacc agttgcgctt tttccaagcc
     5101 ttcggcaacg gcggcggcaa aatggttgcc cagattattc agggctttgg gcgggagttc
     5161 ttcggtaagg agttcgatta aaagggtttg ggtcatcatt cggctttctt tgaatttggt
     5221 taatctgcct gtttataggt ttcgctgtaa ttttcccagc cgtcatcccc gtaaaaacca
     5281 tcaaccagcg gggtggcgta caaagtggcg acatcttcgc ggcctgccag ccaagagata
     5341 atggcttttt tctcggtttc gcccaagctt cgggcaccgg atttttgaaa caggcacgaa
     5401 aaatcgccgc aatcgccccc gcccgccatt tccaagccat ttgcctcaag atacgcaatc
     5461 agctcgtcca taaagcggtc gaacgcttca gcatcgtccg cagcttggtg caaactgcct
     5521 cgaacgccga aaatcaatgc ttgaaactcg cccaaatgca gctttttatg ctggcggcgg
     5581 ttcattttgt gcaggcgttt cctgctcggg gtgcggaaat agacaggcat aattttccta
     5641 aaaaatataa tggcttccgg acggctgcca tatcgtgccg cccgaacgta aaaatcgtcg
     5701 ctcccctagg cggcatttgc cttcattaag ggaaagccca gtttttcgcg gctttcgaca
     5761 tatttttgcg ccacggcgcg gctcaacgcg cggatgcgcc cgatgtaggt ggcgcgttcg
     5821 gttacggaaa tcgcgccgcg cgcgtccaag aggttgaacg tatgccccgc tttgaggacc
     5881 agctcgtagg caggcagggc gagggcggcg ttttcttcgg caagcaggcg tttggcttgc
     5941 gcttcgtagt cgttgaactg gcgcagcagc caatcggcat cgctgtattc gaagttgtag
     6001 gtggattgtt cgacttcgtt ttggtggtac acgtcgccgt agcttacggt gttgccgtcc
     6061 ggcgtttttg cccaaacgag gtcatagacg ttttccacgc cttgcaggta catcgccaag
     6121 cgttcgatgc cgtaggtaat ttcgccgagt acaggcgtgc agtcaatgcc gccgacttgt
     6181 tggaaatagg taaactgggt tacttccatg ccgttgagcc agacttccca gcccaaaccc
     6241 catgcaccga gggtggggtt ttcccagtcg tcttcgacaa agcggatgtc gtggactttg
     6301 gggtcgatgc ccaattcgcg cagggagtcg agatagaggt cttggatatt ggcgggagcg
     6361 ggcttgaggg cgacttggaa ttggtaatag tgttgcaggc ggttggggtt gtcgccgtag
     6421 cgaccgtctt tggggcggcg gctgggttgg acgtaggcgg caaaccaagg ctcgggcccg
     6481 agtgcgcgca ggcaggtggc ggggtgggaa gtacctgcac cgacttccat gtcgaagggt
     6541 tggataacgg tgcagccttt gtctgcccag aatgtttgca gtttgaagat gatttgttgg
     6601 aaggtaagca tggcttatga ttcgataaaa taaagggttt attttactgt ttccattgct
     6661 gtttggatag gtttatctca aagacagact gatttgaaaa cacggcatac atgatatagt
     6721 ggattaaatt
//
LOCUS       DAWKOG010000108         6721 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.108, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000108 DAWKOG010000000
VERSION     DAWKOG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6721
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.108"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(95..727)
                     /locus_tag="WJK66_001546"
     CDS             complement(95..727)
                     /locus_tag="WJK66_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579242.1"
                     /translation="MEKIEKFKSELLNAIFQYTQCISIFVYKKNIYYLIDCKENFILN
                     MKLDLDLDFKNGNITLEQYQDEMNSYYYRNGIWQLTKDNFESYLQSDSVIELKKDELK
                     KLMFQGFTSAEAVRLYSVVENKLSYNDPISDSGQQSDFLKINQISSRLPLFYVNFDTE
                     VYLHMDWDRCHEDYVYDGWFSKAMDFGYLIPDEFCYWKIEGRDYWKFRQL"
     gene            complement(882..1376)
                     /locus_tag="WJK66_001547"
     CDS             complement(882..1376)
                     /locus_tag="WJK66_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdiI family contact-dependent growth inhibition
                     immunity protein"
                     /protein_id="HEZ7579243.1"
                     /translation="MIFKQNQNYWAVFYTNEEALIVQTCSGLGLTAIDHLYPPHILPL
                     DTDNETLGTTVLQALANSRTFVYDSPEDQDFFDTEKIRQRYEDWVAKLCGNLGYKTKR
                     ALFKNMMSVDIWLHNGCLKISPSRHVKLDAWDAIDADDVILSLDNSPEEIGTGLRLAL
                     SRCR"
     gene            1694..3127
                     /locus_tag="WJK66_001548"
     CDS             1694..3127
                     /locus_tag="WJK66_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEZ7579244.1"
                     /translation="MNEYSQLIKHPDISLSPISDSIGIGNPATGEILAYVRNTGSDKL
                     KNLIQKAAAAQKLWAAKTALERADILWRWYFLVKENKEALARLMTMEQGKSLTESRGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIVKPASLTPLSAYALVVLAYEAGVPQDLLPVVSGRASEIGREFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYAQSGIYDEFCRKLSEKVAELKLGNGLEDGVNQGPLIEEKAVEKV
                     EQHIADALAKGAVCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEVEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCMDVG"
     gene            3236..4024
                     /locus_tag="WJK66_001549"
     CDS             3236..4024
                     /locus_tag="WJK66_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HEZ7579245.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALVYGL
                     HSVSLARPVVEAFEQDRMTIVTAAEVVVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEKIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            4392..5948
                     /locus_tag="WJK66_001550"
     CDS             4392..5948
                     /locus_tag="WJK66_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ7579246.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQYAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDSTTALGALELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELASQLNLPQQEEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            5965..6711
                     /gene="surE"
                     /locus_tag="WJK66_001551"
     CDS             5965..6711
                     /gene="surE"
                     /locus_tag="WJK66_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEZ7579247.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEKALWTLLAHFFKTPPQFPILWNIN
                     IPAVAPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGRVSDKEEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAVFWHAD"
BASE COUNT         1659 a         1628 c         1750 g         1684 t
ORIGIN      
        1 agtgttgttt tacaggaaag taaaggccgt ctgaaagggt ttaccagctt tcagacggcc
       61 ttttgaattc atgcaagcta tggcttgctt atatttataa ttgtctaaat ttccaataat
      121 cccgtccttc aattttccaa taacaaaatt cgtccggtat taaataccca aaatccatcg
      181 cctttgaaaa ccagccatca taaacataat cttcatgaca tctgtcccaa tccatatgta
      241 agtacacttc agtgtcaaaa ttgacataga ataaaggcag cctcgaggaa atctgattga
      301 tttttaagaa atcactttgc tgtccagagt ctgagatagg atcattgtac gacagcttgt
      361 tctcgacaac ggaatataac cttaccgctt cagcagaagt aaatccctga aacatcagtt
      421 tcttcaattc atcctttttt aattcaatga ctgaatctga ttgaagatag ctttcaaaat
      481 tgtccttagt taactgccag atgccgtttc ggtaataata actattcatt tcatcctgat
      541 attgttctaa cgtaatatta ccgtttttaa aatctaaatc taagtctaat ttcatgttta
      601 atatgaaatt ttccttacaa tcaatcaaat aatatatatt tttcttatag acaaaaatag
      661 atatgcattg agtatattga aagatggcat ttaataattc agatttaaat ttctcaattt
      721 tttccatatt tgccaaattc cttaatagct ttatcttcag caagtcgtag cccgcattaa
      781 atctgcaggt aaggtgtggt acgcacgcca gtgagcttga gtctaatgaa aaaggtcgtc
      841 tgaaacattt ttccggcttt cagacgacct tttgtcaaat attatcggca gcggctcaat
      901 gctaacctta aacctgttcc gatttcttca ggactgttat ccaatgataa aattacatcg
      961 tctgcatcaa tggcatccca cgcgtccagc ttgacatggc ggctcgggct gattttcagg
     1021 cagccgttgt gcagccaaat atccacgctc atcatgtttt taaatagggc gcgtttggtt
     1081 ttatagccca agttcccgca tagcttggca acccaatcct catagcgttg ccgaattttt
     1141 tcggtatcaa aaaaatcttg gtcttctgga ctgtcataaa cgaaagtcct gctgtttgcc
     1201 aatgcttgca agaccgttgt gcctaaagtt tcattgtcgg tatccaatgg caggatatga
     1261 gggggatata ggtggtctat tgccgttaat cccaaacctg aacatgtttg aacaatcaga
     1321 gcttcttcat tcgtataaaa aactgcccaa taattttgat tctgtttaaa aatcattatt
     1381 ttatctccgt aattttgact gtaatgtttt gacttttgcc atactctacc acacgttgca
     1441 actgcaatct ttgctcctta tatattgcac cccatcaaag ggctgcatta cttttctttc
     1501 taaagattac cattgtataa aaaagtattt ttcttaaacg acagggattg cgtccgcgcc
     1561 atattcagac actgcccgat cggatgcttc agacggcatc ggaagggttt gcagttttag
     1621 aatatgaaga tgataatgtc cgtgagattg acggcatttg aaagcggtta tcccgcatac
     1681 cggaggacac gaaatgaacg aatattccca attaatcaag catcccgata tttccctttc
     1741 cccgatttca gacagcatcg ggatcggcaa tccggcgacg ggtgaaattt tggcgtatgt
     1801 ccgcaacacg ggttcggaca aactgaaaaa cctgattcaa aaggcggcgg cagcgcaaaa
     1861 attatgggcg gcgaaaactg ctttggaacg cgccgacata ttgtggcgtt ggtatttttt
     1921 agttaaagaa aacaaggaag cactggcgcg cctgatgacg atggagcagg gcaaaagcct
     1981 gaccgagtcg cgcggcgaaa tcgattatgc ggcttcgttt gtgcgctggt ttgcggaaga
     2041 ggcgcggcgg attgacggcg atgtgctgac aagtgtgaaa gcgtcgcaaa aactggtcgt
     2101 gttgaaacag cctgtcggcg ttaccgccgc gattacgccg tggaacttcc catctgcaat
     2161 gattgcgcgc aaggctgcgc ctgctttggc ggtgggctgc gcgatgatcg tcaaacccgc
     2221 atcgctcacg cctttgagtg cgtatgcgtt ggtcgtgctg gcttatgaag cgggcgtacc
     2281 acaggatttg ttgcccgtcg tcagcggtcg cgcatcggaa atcggccgtg aatttgccac
     2341 gaacccgatt atccgcaaaa tcagcttcac cggctctacc gaagtcggtg cgaaaatttt
     2401 tgccgacagc gcggcggaca ttaaaaaact cagtttggag ctgggcggca acgcgccgtt
     2461 tatcgtgttt gacgatgccg atttggacaa agccgtcgaa ggtgcgctcg ccagcaagtt
     2521 ccgcaacagc ggtcagacct gcgtctgcac caaccgcgtt tacgcccaat ccggcattta
     2581 cgacgaattt tgtcgcaaat tgagtgaaaa agtagccgag ctcaaattgg gcaacggctt
     2641 ggaggatggc gtcaaccaag ggccgctgat tgaggaaaaa gcggtagaaa aagtcgagca
     2701 gcacattgct gacgcgctcg ctaaaggcgc ggtctgtctg accggcggca aacgcagcgc
     2761 attgggcgga acgttttttg agccgaccgt cttaagcggc gtaacggcgc aaatggcggt
     2821 ggctcgcgaa gaaaccttcg ggcctttgtg tccggtattc cgttttgaaa ccgaagtcga
     2881 ggtcatcgag gctgcgaaca atacggaata cggtttggcg gcctaccttt tcaccgccga
     2941 caccgcccgc caatggcgcg tcggcgaagc cttggaatac ggtatggtcg gcatcaatac
     3001 gggcttaatc agcaatgaag cggcaccgtt cggcggcgtg aaacgcagcg gtttgggacg
     3061 tgaaggcagc aaatacggtg cggacgaata tctggaattg aaatatttgt gtatggatgt
     3121 cgggtgacgg atgccgtctg aacggcaggg tttcagacgg cgccgcattt taagcagtct
     3181 ctatctgttg tacaatgcgc cctgttttta cggttatttt tgatttgaac aagatatgat
     3241 ggaaaacgga aaaacagtcc cgagatggcg ttttgccttg aaaagtgcgg gctggcatct
     3301 cttaatcagc ctgtcggttg cagggctggc ggcattgctg gtttttaagg tttggtatcc
     3361 ttatccttat gccgagctga cgggagggct gtcgctttat cagctggtgg tggctgtcga
     3421 tattgtatgt ggtccgctgc tgactttaat tttggcaagc ccgaagaaaa agacaaaggc
     3481 acgcatggtc gatttttcca tggtcggcat catccagctg gcggctttgg tgtacggtct
     3541 gcacagcgtt tcgctggcgc gtcctgtggt ggaagcgttt gaacaggatc gtatgaccat
     3601 tgttacggcg gcggaagtcg tggtcgaaga tttgcacaaa gcccccgaag ggctgcaaag
     3661 cctgtcgtgg ttcggcatcc gccgcattgc attgaaagaa cctgaggatg cggatgagaa
     3721 gaacaagacg ctggatttgt ccctgaaagg tatcgagccg agtatgcgtc ccgaccggtg
     3781 gctgccgtat tccgacaagg aagcagaaaa aatccgcaaa catctgaaac cgctgaaagt
     3841 cttggcggat gcgagaaaaa cgacggttgc ggacattctg aaacaggcag gtctcgccga
     3901 aggggaggag ctgtattacc tgccgtttac cagcagcagg cagaaagagt ggatagtcat
     3961 taccgataaa gagggcaaca ccaaaggcta cgcgccgata gacggcttca tcatcacccc
     4021 ttaagcgttg ggactccgtc cgcactcgaa catccgttct tcgcggcggt agaatcagac
     4081 tgtatttgag aggggcgcaa cccctcaatc agggcaacac ataggggaag gctttatgtg
     4141 ccgtccgtgt gttgaaacat ttcaaaataa gctcatgccg tccgaacatc ctttcagacg
     4201 gtatggcatt ttgccattgc cccgataagc tgttaaacta tttaaattat ttcgaccgaa
     4261 ggtacacgca catgcaagac cccgagcaga gcagcaagcc cgcccgccgt tttttgtgcg
     4321 taccgtctgt tcaggcagct tgagcgcttc aggctgcctt ttcgcgtttg aacttgagga
     4381 atacgaaaat catggatttc agttggttgg cagaaccgca tacctggata ggttttgcca
     4441 cgcttttggt gttggaagtc gtattgggga tagacaacct tgtctttgtg gcgattttgg
     4501 caaacaaggt ccagcccgca cggcgcgacc gagcacggat tatcgggctg gggctggcag
     4561 tcgtcatccg catcattatg cttgctttta tggcgcacat catcacgctg accgagccgc
     4621 tgttccaaat cggcggcctc gccgtttccg gcaaggacat gattatgctc gcgggcggta
     4681 ttttcctgct ttacaaagcc accaccgaac tgcatgaacg cctcgaaggg cacaaccgtt
     4741 ttaccgttgc cgacaaccaa aaaaaacacg cgccgttttg gggcgtggtc gcgcaaatcc
     4801 tgatactgga tgccgtgttt tccatcgatt cggtcattac tgcggtggcg atggtcgatc
     4861 atatcgtcgt ggcgatgggt gcggtcgtcg tcgcgatggc tgtaatgatt tctgccagca
     4921 aactcttgac cgaatttgtc gacagacacc ctaccgtcgt gatgctctgc cttggttttt
     4981 tgttgatgat cggtttcagc ctgattgccg aagccttcca tttccacatt cccaaaggct
     5041 acctctacgc cgccatcggc ttctcgattt taatcgaact gtttaaccag atttcccagc
     5101 gcaacagccg caaaaacgac tacatcggca gctcgtggcg caaacggact gctgaaaacg
     5161 ttttgggtat gatgggcata cgcgaaagcg tgcttgccga cgcgggcggc gaatccgggg
     5221 acgacgcgca ttttgaagaa aacgaaaaat cgatgatacg cagcgtgctg acgcttgccg
     5281 aacgcccgat tatgggggtg atgatcccac gccgcgacat cgaacggctg gacatttccc
     5341 aaagccgcga agaacagtat gcccaactgc aaaacacgcc ttacagccgc ctgctcgttg
     5401 tcggaaaggc gggcgtggac gaacctttgg gctacatcaa caaaaaagac ctgctgtccc
     5461 aactgctgga aacaggcggt ctcgacattc agacggcatt gcgccagccg ctcgttttgc
     5521 ccgacagcac taccgcgctg ggcgcgctcg aactcttccg ccaaagcagc gcggattatg
     5581 ctttggtggt ggacgagttc ggcgcggtat tgggcatggt aaccatgaaa gacctgctcg
     5641 aaaccatcgc aggcgagttc cccgaagaat ttgagcgcga agaagaacca gccgttcagg
     5701 ggaatcccga tgaaagcctg acggtggaag gcgcgttgga atatgtggag ctggcatccc
     5761 aactcaatct gccgcagcag gaggaagatg ccgatttcca tacggttgcc gggctgatta
     5821 tggaagaatt gcaaaccatc cccgatgtcg gcgattttgc cgatttccac ggctggcggt
     5881 ttgaagtggt cgaaaaagaa gggcagcgca tcgagcgggt caaaatcacc aaattgcccg
     5941 aagaataagc attcaggata gaaaatgaac gttttgattt ccaacgacga cggctacctc
     6001 tccgaaggta ttgccgtttt ggcgcgcgtt acggcggaat ttgccaacgt cagggtggtt
     6061 gcgcccgaac gcgacaggag cggggtcagc aattcgctga cgctggaacg ccctttgcag
     6121 ttgaaacagg cgcaaaacgg gttctactat gtcaacggca cgccgaccga ctgcatccac
     6181 atcgggcagt ctgtattttc ggattttcag gccgattttg tcttttcggg catcaaccgg
     6241 ggcgcgaata tgggggacga cacgctttat tcggggacgg ttgcggcggc aaccgaagcc
     6301 taccttatgg gcatacccgc tgtggcgttt tccttaaacg acgcttccgg acgctattgg
     6361 gcaaccgcag aaaaggcact gtggacattg ttggcgcatt tttttaaaac ccccccgcag
     6421 ttccctattt tgtggaacat caatatcccc gccgtcgcgc cggaagatgt gcggggcatt
     6481 aaaatcgccc gtttgggcag gaggcatcac ggtcagaacg tcatccccgc gcgcaatccg
     6541 cgcggcgaac agatttattg gataggcccg gtaggcaggg tttccgacaa agaagaggga
     6601 acggatttcg gtgaatgcgg cgcaggtttc attaccgtaa cgccgctgca aatcgacctg
     6661 accgcctatc cggacatggc ggaaacagcg gtgttctggc atgcggactg accgtttcat
     6721 c
//
LOCUS       DAWKOG010000109         6603 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.109, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000109 DAWKOG010000000
VERSION     DAWKOG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6603
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.109"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..738
                     /locus_tag="WJK66_001552"
     CDS             <1..738
                     /locus_tag="WJK66_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HEZ7579248.1"
                     /translation="NTDFSDMTPEQLDLEVKKITAYAKLAAGTVAGVTGGDVNTAAQT
                     AQNAVENNAVKAVVTAAKVVYKVARKGLKNGKINVRDLKQTLKDEGYNLADNLTTLFD
                     ETLDWNDAKAVIDIVVGTELNRANKGEAAQKVKEVLEKNRPYIPNKGAVPNMSTYMKN
                     NPFGKQLAQISEKTTLPTQQGQSVFLVKRNQGLLKTGDRFYLDGQHKNHLEVFDKNGN
                     FKFVLNMDGSLNQMKTGAAKGRKLNLK"
     gene            747..1124
                     /locus_tag="WJK66_001553"
     CDS             747..1124
                     /locus_tag="WJK66_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579249.1"
                     /translation="METLNDIKKILINVGLYQGFDLTDPKVSEEVNHETANMKWIKDY
                     TSDGNWDNEFKEDLKNFLDYMEVCQLALNDKNFKIASNSLFMAMIYAGNLSLIFDSIK
                     TDISTLLSAEYKKNSFSWPSLDE"
     gene            1402..1776
                     /locus_tag="WJK66_001554"
     CDS             1402..1776
                     /locus_tag="WJK66_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579250.1"
                     /translation="MKNFNVVKESLRELGIKQGFDLYEKATTEKLNSEDPLDLQWLSN
                     YSSDWNDELEEDFDSFFQHMKEYQYAIDNEDIKSACSSLCEAMLYVGNIKNFFEFLKS
                     DMIRLLRGESKTTDFQWPQFDE"
     gene            1811..3406
                     /locus_tag="WJK66_001555"
     CDS             1811..3406
                     /locus_tag="WJK66_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ7579251.1"
                     /translation="MRKVCASARTRSMIYGSMPSEKLTIFQTAFVMQVNIQIPYILPR
                     CVRAEDTPYACYLKQLQVTKDVNWNQVQLAYDKWDYKQEGLTGAGAAIIALAVTVVTA
                     GAGAGAALGLNGAAAAATDAAFASLASQASVSLINNKGDVGKTLKELGRSRTVKKLVV
                     AAAIAGVSNKIGASSLATWSETPWVNNLNVNLANAGSAALINTAVNGGSLKDNLEANI
                     LAALVNTAHGEAASKIKQLDQHYIAHKIAHAIAGCAAAAANKGKCQDGAIGAVVGEIV
                     GEALVKNTDFSGMTASEIEKAKANITAYAKLVAGATVGVTGGNVDVAANASETAVKNN
                     ALDIIWDIGNLVWDGGKWIYAKSIGDKQMAREAAIDFGVDAAAAAVPFVPAGATKISR
                     GGAYVLKAGDEAVDTAKAIQEIQKQTGIKLTYDKVNKVWTTPAGLDYGLDAKHGNRIK
                     HVLAHTIPNPNKPVHSVFNVSRKEVLPLVDEAWRMKGNPLPNDSSVYLVDMKKPIGTK
                     GETKVRIVVQKGTNKIISAYPQK"
     gene            3425..3688
                     /locus_tag="WJK66_001556"
     CDS             3425..3688
                     /locus_tag="WJK66_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579252.1"
                     /translation="MDKEIKICPRCEQGYLYHAKPKYFSGEVILCDECYAMWLGDMKI
                     FYGQYGKDFYDYHEFMKDKGIEEINMWEGELFDHPYYEDEKFK"
     gene            3795..6080
                     /locus_tag="WJK66_001557"
                     /pseudo
     CDS             3795..6080
                     /locus_tag="WJK66_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246826.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
     gene            6096..6527
                     /locus_tag="WJK66_001558"
     CDS             6096..6527
                     /locus_tag="WJK66_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579253.1"
                     /translation="MKNNIFLNLNKKSINNNHFVISIFFETIYQFETKDTLLECFKNI
                     TTTGHFGVIGAQYEKIDATRWIGDYEEVNGFEYIDKAPSIYFSVGDDFNPEELIIPIN
                     LAYHYFNIAISDFLIAHPEYQKKCKEIQKTYSQTSCSLHET"
BASE COUNT         2198 a         1253 c         1495 g         1657 t
ORIGIN      
        1 aataccgatt ttagcgatat gaccccggaa caattagatc tggaagttaa gaaaattacc
       61 gcctatgcca aacttgcggc aggtacagtt gcaggcgtaa cgggaggaga tgtcaatact
      121 gctgcacaaa ccgcacaaaa cgcggtagaa aataatgcgg ttaaagctgt tgtaactgct
      181 gcaaaagtgg tttataaggt agccagaaaa ggattaaaaa acgggaaaat caacgttaga
      241 gatttaaaac agacgttgaa agacgaaggt tataatttag ccgacaacct gaccacctta
      301 ttcgacgaaa cattggattg gaacgatgcc aaagccgtta ttgatattgt cgtcggaaca
      361 gagctgaatc gcgctaataa aggggaagcg gcacaaaagg tcaaggaagt tttagaaaaa
      421 aatcgtcctt atatccctaa taaaggtgct gtaccgaata tgagtacata catgaaaaat
      481 aatccttttg gaaaacagct ggctcaaatt tcagaaaaga caacgcttcc gacgcagcaa
      541 gggcagtctg tcttcttggt aaaaagaaac caagggttat taaaaaccgg tgataggttt
      601 tatttagatg gccaacataa aaatcattta gaggtttttg ataaaaatgg gaactttaag
      661 tttgttctaa atatggatgg ttcgcttaac caaatgaaaa ctggggcagc aaaaggtcgt
      721 aaattaaact taaaatagga aattttatgg aaacattgaa tgatataaaa aaaatcttga
      781 ttaatgtggg gctttatcaa gggtttgatt tgacagatcc aaaagtatca gaagaagtta
      841 atcatgaaac agctaatatg aaatggatta aagattatac ttcagacggg aattgggata
      901 atgaatttaa ggaggattta aaaaactttt tagattatat ggaagtatgc caattagccc
      961 taaacgataa aaatttcaaa attgccagta attctttatt tatggctatg atttacgcag
     1021 gtaatctatc tcttatattt gattcaataa aaactgatat atcaacatta ttgagtgctg
     1081 agtataaaaa gaatagtttt tcatggccat ctcttgatga atagaaagca agttgtagcc
     1141 tgcatgaaat ctaaaaccca tgcataaggt gtgggcttca gtatacgcgt tccatgattt
     1201 acggccatat gccgtctgaa aagctcaatt ttttcagacg gcatttgtta tgaaagtaaa
     1261 tatttagatt ccctgtatac tgtttagact cgtgtgtgct gagtaagctg tagtctgcat
     1321 gaaacctaaa actcgctcaa aattaagcta agacattagc agggcaaggg cgaaaattga
     1381 atcttaaata aggtgattca gatgaaaaat tttaatgtag taaaagaaag tttaagagag
     1441 ttaggaatta aacaaggatt tgatctttat gagaaagcca caactgaaaa attgaatagt
     1501 gaagatcctc ttgacttaca atggctttct aactattcat ctgattggaa tgatgaatta
     1561 gaagaagact ttgattcttt ttttcagcat atgaaggaat atcaatatgc tattgacaat
     1621 gaagacatta aatctgcatg tagttcacta tgtgaagcta tgctctatgt tggtaatatt
     1681 aaaaattttt ttgagtttct caaaagcgat atgattagac tgttgagagg tgaaagtaaa
     1741 acaacagact ttcaatggcc gcaatttgat gaatagcagc aagctgtagc ctgcatgaaa
     1801 cctaaaatcc atgcgtaagg tgtgtgcttc agcacgcacg cgttccatga tttacggctc
     1861 aatgccgtct gaaaagctca caatttttca gacggcattt gttatgcaag taaatattca
     1921 gattccctat atactgccca gatgcgtgcg tgctgaagac accccctacg cttgctattt
     1981 gaaacagctc caagtcacca aagacgtcaa ctggaaccag gtacaactgg cgtacgacaa
     2041 atgggactat aaacaggaag gcttaaccgg agccggagca gcgattattg cgctggctgt
     2101 taccgtggtt actgcgggcg cgggagccgg agccgcactg ggcttaaacg gcgcggccgc
     2161 agcggcaacc gatgccgcat tcgcctcgct ggccagccag gcttccgtat cgctcatcaa
     2221 caataaaggc gatgtcggta aaaccctgaa ggagctgggc agaagccgca cagtgaaaaa
     2281 actggttgta gcagcggcca tcgcaggcgt atccaacaaa atcggtgctt cctcccttgc
     2341 cacttggagc gaaacccctt gggtaaacaa cctcaacgtc aacttggcta atgcgggcag
     2401 tgccgcgctg attaacaccg ccgttaacgg cggcagtctg aaagacaatc tggaagcgaa
     2461 tatccttgcg gctttggtga atactgcgca tggagaggca gcaagtaaaa tcaaacaatt
     2521 ggatcagcac tacattgccc ataagattgc ccatgccata gcgggctgtg cggcagcggc
     2581 ggcgaataag ggcaagtgtc aggacggcgc gattggtgcg gtcgtggggg agattgtcgg
     2641 ggaggctttg gttaagaata ccgatttcag cggtatgact gcttctgaaa ttgaaaaagc
     2701 taaagcgaat attactgcgt atgcaaaatt ggtagccgga gcgactgtag gtgttacagg
     2761 aggcaatgtt gatgtggcgg caaatgcttc cgaaacagct gttaaaaata atgcattaga
     2821 tattatttgg gatatcggca acctcgtatg ggacggcggt aaatggattt acgccaaatc
     2881 tattggcgat aagcagatgg ctcgagaagc ggcgattgat tttggtgtgg atgccgccgc
     2941 agctgccgtt ccctttgttc cggcaggtgc gactaaaatc agccgaggcg gggcttatgt
     3001 tctgaaggcg ggagacgaag cagttgatac ggctaaagcc atacaggaaa ttcagaagca
     3061 gaccggaatc aagcttactt atgataaggt taataaggtt tggacaacac cggcggggtt
     3121 agattatggg ttagatgcta agcatggtaa taggattaaa catgttttag cccatacaat
     3181 tccaaatcca aacaaacctg ttcattctgt ttttaatgtg tcccgtaaag aagttttgcc
     3241 tttggttgat gaagcttgga gaatgaaagg aaatcctttg ccaaatgatt catccgtata
     3301 tcttgtagat atgaagaaac ctattggaac aaaaggagaa acaaaagtgc ggattgttgt
     3361 gcaaaaagga acaaataaaa tcatttctgc atatcctcag aaataattaa gaaaggaatc
     3421 tcttatggat aaagaaatta aaatttgccc aagatgtgag caaggctacc tttatcatgc
     3481 aaagcctaaa tatttctctg gggaggtcat tttatgcgat gaatgttatg ctatgtggct
     3541 tggggatatg aaaatttttt acggacaata tggaaaagat ttttatgatt atcatgagtt
     3601 tatgaaagat aaaggcatag aagaaataaa tatgtgggaa ggagagcttt ttgatcaccc
     3661 atattatgag gatgaaaaat ttaaataatt gattttctgt tccccgaatt tgggaaatac
     3721 gatgatattt taaacccaaa tattatttaa agtagcaata ggccgtctga atatccgttt
     3781 ttcagacggc ctcaatgcaa ctgctggcag ccgaaggcat tcaccaacac caattgaatg
     3841 ttcagaaaag tacccgtttc atcggcatca aagtgggtaa aagcaattac agcaaaaacg
     3901 agctgaacga aaccaaactg cccgtacgcg ttatcgccca aacagccaaa acccgttccg
     3961 gctgggatac cgtactcgaa ggcaccgaat tcaaaaccac cctttccgga gccgacatac
     4021 aggcaggggt gggtgaaaaa gcccgagccg atgcgaaaat tatcctaaaa ggcatcgtta
     4081 accgcatcca aaccgaagaa aagctggaat ccaactcgac cgtatggcaa aagcaggccg
     4141 gaagcggcag cacggttgaa acgctgaagc taccgagctt tgaagggccg gcactgccta
     4201 agctgaccgc acccggcggc tatatcgccg acatccccaa aggcaacctc aaaaccgaaa
     4261 tcgaaaagct ggccaaacag cccgaatatg cctatctgaa acagctccaa gtagcgaaaa
     4321 acatcaactg gaatcaggtg cagcttgctt acgacagatg ggactacaaa caggagggct
     4381 taaccgaagc aggtgcggcg attatcgcac tggccgttac cgtggtcacc tcaggcgcag
     4441 gaaccggagc cgtattggga ttaaacggtg cggccgccgc cgcaaccgat gcagcattcg
     4501 cctctttggc cagccaggct tccgtatcgt tcatcaacaa caaaggcgat gtcggcaaaa
     4561 ccctgaaaga gctgggcaga agcagcacgg tgaaaaatct ggtggttgcc gccgctaccg
     4621 caggcgtagc cgacaaaatc ggcgcttcgg cactgaacaa tgtcagcgat aagcagtgga
     4681 tcaacaacct gaccgtcaac ctagccaatg cgggcagtgc cgcactgatt aataccgctg
     4741 tcaacggcgg cagcctgaaa gacaatctgg aagcgaatat ccttgcggct ttggtgaata
     4801 ctgcgcatgg agaggcagcc agtaaaatca aacagttgga tcagcactac atagtccaca
     4861 agattgccca tgccatagcg ggctgtgcgg cagcggcggc gaataagggc aagtgtcagg
     4921 atggcgcgat aggtgcggct gtgggcgaga tagtcgggga ggctttgaca aacggcaaaa
     4981 atcctgacac tttgacagct aaagaacgcg aacagatttt ggcatacagc aaactggttg
     5041 ccggtacggt aagcggtgtg gtcggcggcg atgtaaatgc ggcggcgaat gcggctgagg
     5101 tagcggtgaa aaataatcag cttagcgaca aagagggtag agaatttgat aacgaaatga
     5161 ctgcatgcgc caaacagaat aatcctcaac tgtgcagaaa aaatactgta aaaaagtatc
     5221 aaaatgttgc tgataaaaga cttgctgctt cgattgcaat atgtacggat atatcccgta
     5281 gtactgaatg tagaacaatc agaaaacaac atttgatcga tagtagaagc cttcattcat
     5341 cttgggaagc aggtctaatt ggtaaagatg atgaatggta taaattattc agcaaatctt
     5401 acacccaagc agatttggct ttacagtctt atcatttgaa tactgctgct aaatcttggc
     5461 ttcaatcggg caatacaaag cctttatccg aatggatgtc cgaccaaggt tatacactta
     5521 tttcaggagt taatcctaga ttcattccaa taccaagagg gtttgtaaaa caaaatacac
     5581 ctattactaa tgtcaaatac ccggaaggca tcagtttcga tacaaaccta aaaagacatc
     5641 tggcaaatgc tgatggtttt agtcaaaaac agggcattaa aggagcccat aaccgcacca
     5701 attttatggc agaactaaat tcacgaggag gacgcgtaaa atctgaaacc caaactgata
     5761 ttgaaggcat tacccgaatt aaatatgaga ttcctacact agacaggaca ggtaaacctg
     5821 atggtggatt taaggaaatt tcaagtataa aaactgttta taatcctaaa aaattttctg
     5881 atgataaaat acttcaaatg gctcaaaatg ctgcttcaca aggatattca aaagcctcta
     5941 aaattgctca aaatgaaaga actaaatcaa tatcggaaag aaaaaatgtc attcaattct
     6001 cagaaacctt tgacggaatc aaatttagat catattttga tgtaaataca ggaagaatta
     6061 caaacattca cccagaataa tttaaaggaa aaattatgaa aaataatatt tttctaaact
     6121 taaataaaaa atctataaat aacaaccatt ttgttatttc gatttttttt gaaacaattt
     6181 accaatttga aactaaagat acgcttttag agtgttttaa aaatattaca actaccggac
     6241 attttggagt aataggtgct caatatgaaa aaatagatgc taccagatgg attggagatt
     6301 atgaagaggt aaatggattt gagtatattg ataaagctcc ttctatttat ttttcagttg
     6361 gagatgattt caatcctgaa gaattaatta tacctattaa tttagcatat cattacttta
     6421 atattgcaat atctgatttc ttaatagctc accctgaata tcaaaaaaag tgtaaagaaa
     6481 tacaaaaaac atattctcaa acaagctgta gcctgcatga aacctaaaat ccatgcgtaa
     6541 ggtgtgtgct ccagcacgca cgcgttccat gatttacggc tcaatgccgt ctgaaaagct
     6601 caa
//
LOCUS       DAWKOG010000110         6532 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.110, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000110 DAWKOG010000000
VERSION     DAWKOG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.110"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(42..962)
                     /gene="truB"
                     /locus_tag="WJK66_001559"
     CDS             complement(42..962)
                     /gene="truB"
                     /locus_tag="WJK66_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEZ7579254.1"
                     /translation="MTNKPAKRPVNGVLLLDKPEGLSSNTALQKTRHLFRAEKAGHTG
                     VLDPLATGLLPVCFGEATKFAQYLINADKAYTATLKLGEASSTGDAEGEIVATARADI
                     SLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKARDITVYAIDIA
                     EFDAPKAVIDVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEAL
                     ANLDETERDSLLLPCDVLVAHFPQTVLNDYAVHMLRCGQRPRFEEDLPSDTPVRVYTE
                     NGRFVGLAEYQKEICRLKALRLMNTAASAA"
     gene            complement(1020..1544)
                     /locus_tag="WJK66_001560"
     CDS             complement(1020..1544)
                     /locus_tag="WJK66_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579255.1"
                     /translation="MNNLIPEHLAAYAHSDSLQIEGVHRCFSLSCQGRDTFHIRYYGE
                     PFDGLMTDTDKAPVKIVAVEAVSGDEIVLFDGAEHGYNAMFCDKYSPNQKQNRTLTDL
                     DEYTYRVLIHLYYNIDYEDEYEDFVNSEGQVPLIDGRIISFDSLKRNGFDAISVDLID
                     EKHSVRELLNEELS"
     gene            complement(1555..1926)
                     /gene="rbfA"
                     /locus_tag="WJK66_001561"
     CDS             complement(1555..1926)
                     /gene="rbfA"
                     /locus_tag="WJK66_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEZ7579256.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTVLNQDAREITEEVLEHARGHLRSELAKRIKLFKIPELHFKYDESL
                     ERGLNLSALIDQVAAEKPVED"
     gene            2117..3361
                     /gene="clpX"
                     /locus_tag="WJK66_001562"
     CDS             2117..3361
                     /gene="clpX"
                     /locus_tag="WJK66_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEZ7579257.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPSESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLQGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            complement(3426..4619)
                     /locus_tag="WJK66_001563"
     CDS             complement(3426..4619)
                     /locus_tag="WJK66_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEZ7579258.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTEGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVANSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGIVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINAPETLHNVRSQGQKLQTALLDLGRKTGLFSQVRGMGLLLGCVL
                     DAPYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLNDEDTAEGLRRLEHVLTEFA
                     AANRP"
     gene            5023..5523
                     /locus_tag="WJK66_001564"
     CDS             5023..5523
                     /locus_tag="WJK66_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579259.1"
                     /translation="MNAMPQTAAQNETLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            5608..6162
                     /locus_tag="WJK66_001565"
     CDS             5608..6162
                     /locus_tag="WJK66_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579260.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSAHPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
BASE COUNT         1576 a         1710 c         1730 g         1516 t
ORIGIN      
        1 caacacatta ttcaaacaca gcccgtattt ttaaccgtcg ttcaggcggc ggatgccgcc
       61 gtgttcatca ggcgcaaggc tttcagacgg catatttcct tttgatattc cgctagaccg
      121 acaaagcggc cgttttccgt gtaaacgcgt accggcgtat cggaaggtag gtcttcctcg
      181 aaacgcggcc gctgcccgca gcggagcata tggacggcat aatcgtttaa aacggtttgg
      241 ggaaaatggg caaccaatac gtcgcagggc agcagcaggc tgtcgcgttc tgtttcgtcc
      301 aagtttgcca aggcttcgag cgtgtggctt tgggcgatgg taaagccggc ggtttcggtg
      361 cggcgcaggg cggtcaggtg ggcgaacgtg ccgatgtgtt tggcgatgtc ttcgctgagg
      421 gtgcggatat aggtgccttt gctgcaacgt acgtcgatga cggctttggg cgcgtcaaat
      481 tcggcaatat cgatggcgta aacggtaatg tcgcgcgcct tgcgttcgat gacgatgcct
      541 ttgcgggcgt attcgtacag cggtttacct tcgtgcttga gcgcggaaaa catcggcggc
      601 acttggcgga tgttgcctgt cagtgcttgg caggccgtct gaaattcggc taaggaaata
      661 tcggcgcggg cggtggcaac gatttcgcct tcggcatcgc ccgtgctgct ggcttcgccg
      721 agtttcagtg tggcggtgta ggctttgtcg gcattaatca gatattgggc gaacttggtc
      781 gcttcgccga agcagacggg caaaagcccg gttgccaaag gatcgagcac gccggtatgt
      841 ccggcttttt cggcgcggaa caagtgcctc gttttttgca gggcggtgtt gctggaaagg
      901 ccttcgggtt tgtcgagaag aagaacgccg ttgaccgggc gtttggcggg tttattggtc
      961 atattaatta gggaatatta atgttcagac cgtctgaaag ggttcggacg gcctgcgtgt
     1021 cagcttaatt cttcattcaa taattcacga acagagtgtt tttcatcaat tagatcaacg
     1081 ctgattgcat caaagccatt tcgttttaat gagtcaaaac taatgattcg accatcaatt
     1141 aaggggactt gtccttcaga attgacgaaa tcttcatatt catcttcata gtctatattg
     1201 taataaagat ggatcagaac tcggtaagta tactcatcca aatcagttaa cgttctgttt
     1261 tgcttttgat ttggactata tttgtcgcaa aacatagcgt tatagccatg ttccgcgcca
     1321 tcaaacaata cgatttcatc gccgcttaca gcttctactg ccacaatttt taccggagcc
     1381 ttatcagtat cggtcatcaa tccatcaaaa ggctctccat agtaacggat gtggaaagta
     1441 tctctacctt ggcaggataa tgaaaagcaa cgatgcacgc cttcaatttg caggctgtca
     1501 ctatgtgcat aggcggctaa atgttcggga atcagattgt tcattttagc agtatcagtc
     1561 ttcaaccggt ttttccgccg ccacttgatc gataagggcg gacaggttca aaccgcgttc
     1621 caacgattcg tcgtatttga aatgcagctc ggggattttg aacagcttga tgcgtttggc
     1681 caattcgctg cggaggtgtc cgcgtgcgtg ttccagcact tcttccgtaa tttcgcgcgc
     1741 atcttggttt aaaacggtgt agaacacggt ggcgtggctg taatcgcggg taacttcgac
     1801 ctcgttgacg gtgatgaagc cggcgcgggg gtctttcagt ccggtacgga cgagttcggc
     1861 aagctcgcgc atgatttgtt ctttgacacg gtcttggcgg gcgtagccgc gttggggttt
     1921 tctcatgaat atcctcagat ttcagatttg gtttcagacg gcatttttgc cgccggggcg
     1981 gttgcgaaag ggcgtattat agcctaatcg gcgggatttt aaggtaatac attgataaga
     2041 acggatatag ttattatact tggcaacccg aacggcacgt gccgttttct gtaacgcata
     2101 cattgtaaag aaagccatgt ccaacgaaaa ccgtacctgt tccttttgcg gaaaatccaa
     2161 atcacacgtc aaacatttaa ttgagggcga aaacgccttt atctgtgacg aatgcgtctc
     2221 aaactgtatt gaaatattgc atgaagacgg aaatgacggc acgccttcgg aaagtgccgg
     2281 aggggagccg gaagaatccg gcaagctgcc cacgcccgcc gaaatcgtcg ccaacctcaa
     2341 cgaccacgtt atcggtcagg aacaagccaa gaaagccttg gcggtgtcgg tgtacaacca
     2401 ttacaaacgc ctgcgccacc cgaaagccgg agctaatgtc gaattgtcga aatccaacat
     2461 cctgcttatc ggcccgaccg gatcgggtaa aaccctgctg gcgcaatctt tggcgcgcaa
     2521 actggatgtg ccgttcgtga tggcagatgc aaccacgctg accgaagcag gttatgtcgg
     2581 cgaagatgtc gaacagatta ttaccaaact gttgggcaaa tgtgatttcg atgtcgaaaa
     2641 agcccagcgc ggcattgtct atatcgacga aatcgataaa atttcacgca aaagcgacaa
     2701 cccgtcgatt acccgcgacg tatctggcga aggtgtgcaa caagccttgt tgaaactgat
     2761 tgaaggtaca gtggccagcg ttccgcccca aggcggccgc aagcatccga atcaggaatt
     2821 tatcaacgtc gataccacca acatcctgtt tatctgcggc ggcgcgtttg caggcttgga
     2881 aaaagtcatc cgccagcgta ctgaaaaagg cggtatcggt ttcggtgcgt ccgttcacag
     2941 caaggacgaa aatgccgaca ttaccaagct gttcggcatc gtcgaaccgg aagacttgat
     3001 taaattcggt ctgattccgg aattaatcgg acgtttgccc gtgattgcaa cactagaaga
     3061 actggatgag gacgcgctga ttaatatttt gaccgagccg aaaaatgctt tggtcaagca
     3121 gtatcaagcc ttgttcggta tggaaaatgt cgagttggaa tttgaagaag gcgcattgcg
     3181 ttccatcgcg cgtcaggcaa tggagcgcaa aaccggcgcg cgcggcctga ggtccatcgt
     3241 cgaacgctgc ctgctcgata cgatgtaccg cctgcctgat ttgcaaggct tgaaaaaagt
     3301 ggtggtcggt aaggcagtca tcgaagaggg cagggaaccg gaattggtgt tcgagtcctg
     3361 atggcaaaca aaaccgcttc ggaacgttga accacgtttc cgaagcggtt ttttgttttt
     3421 gatgcctagg ggcggtttgc ggcggcaaat tccgtcagca cgtgttccaa acgccgcaaa
     3481 ccttccgccg tatcctcatc gttcaacagc agcgaaggcg cgaaacgcaa tacgtccgca
     3541 cccgcaacca aaatcatcac gccgtgtttc aaggcggcgg cggttatttc ggatgcgcgt
     3601 ccgcgataag gcgcgtccaa cacgcagccg agcagcagtc ccatcccgcg aacctgtgag
     3661 aacaagcctg ttttcctgcc caaatccagc aatgccgtct gaagtttctg cccctggctg
     3721 cggacgttgt gcaaggtttc cggcgcgttg atgatgtcga aggcgcggct gccgaccgcg
     3781 cacgccatcg ggttgccgcc gaaagtcgag ccatgcgtgc ccggttggaa ggcggcggca
     3841 atcttttctg tcgccagcat cgcgccgatc ggaaagccgc agcccaaggc ttttgccgaa
     3901 ctcaaaatat cgggaacaat gccgtaatgt tcataggcaa acagcctgcc cgtatgcccc
     3961 atcccggttt gaacttcgtc caaaatcaac aacgcattgt gccggtcgca cagacggcgc
     4021 gcggtttgca aatattccgc agtggcgggc aggatgccgc tttcgccctg tatcggctcg
     4081 atgatgaccg cgcaggtctg ttcgccgacg gcagcttcca gcgcggcaat atcgttgaac
     4141 ggaacgtgcg taatgccttg cggcaggggt gcataatcct tgctgtattt cggctggccg
     4201 ccgacggaca cggtaaacag cgtgcgtccg tggaaactgt tgatacaggc gacgatttcg
     4261 ctttttcctc cgccgaaacg gtcgcgcgca tatttccttg ccagcttcaa cgccgcctca
     4321 ttcgcttccg cgcccgagtt gcagaaaaaa accttgtcgg caaaactgtt tgcaaccaat
     4381 ttttgcgcca attcctgcgc cggacgcgtc gtatagatat tggaaatgtg ccacagcttg
     4441 tgcatctgcg cgtttaaagc atcgacaagg gcagggtggc agtgtcccag cgcattgacg
     4501 gcgataccgc ctgaaaaatc aatatattca cgcccttccg tatcccaaac gcggctgcct
     4561 gaagcgcgtt cgggaatcat cggggcaaag gcgaaattgg gggtcagata gttttgcata
     4621 attgctcctg attgcggttt tgctcgggat tatgcaccgt ttttcaaagc agataaaggg
     4681 aaattgccga caatatcggg cagcggtaaa aataaaccga aatattttca atcggatagg
     4741 tgaaaaatca aaaaaatttc gataaaatgc ttgaggcgcg gcagcgtttt gatttagaat
     4801 ctccccaata tttttatctt cagacggcat ttcatcatgc aaggcaaaaa atttacaacc
     4861 gcttattgct attggtacca cacagacgtg cggtactttt ttgcgcttgc ttgaaatcca
     4921 tcccatacgc aaaacaggcc gctcgaaaac ggggcggctt ttttgttacc ttcacacagg
     4981 tcgcccgatt cggcaacaca cgacagaatc aggaaaatga ccatgaatgc aatgccccaa
     5041 accgccgcac aaaacgaaac cctgcacact tgtagcatta ttgtcgatga tgcacaggat
     5101 ttttccccca tgcttctcga tgcagggcgg gacgacacac tcatcagcga atcgatgatt
     5161 ccgcaaatcc gcactgttgc cgccctgata gccgccgaac ggcacgactt cagccgcagc
     5221 agccccgccg agtttaccga tgccgccgat ttttttgccg cacggattct cgtattgggc
     5281 gtgcgacgct tccatctgga cgtatcgctg cttcagattt tgaaaaccgc caacagccgc
     5341 gcccgccgct ttgccgaaaa acaccgcctg tttttcacac ccgcacaagc cgaattgagc
     5401 ctgaacaaac acaaaaaccg ccgcctgctg accgtagaaa ccgaacacga agtcgaaaac
     5461 aaaggcaacc cagtcgccaa cagcctcgca tttgtccgca aactgcacac cctgcccctg
     5521 taaacatcac gatttgtcgg cagaatgctt gccaaaattc ccgacggcga tacaataaaa
     5581 ccgattttcc acccaaacag gacacacatg aaaaaaatca tcgcctccgc gcttatcgca
     5641 acattcgcac tcgccgcctg ccaagacgac acgcaggcgc ggctcgaaca gcagcagaaa
     5701 cagattgaag ccctgcaaca gcagctcgca cagcaggcag acgatacggt ttaccaactg
     5761 actcccgaag cagtcaaaga caccattcct gccgaagcac aggcaaacgg caacaacggg
     5821 caacccgtta ccggtaaaga cgggcagcag tatatttacg accaatcgac aggaagctgg
     5881 ctgctgcaaa gcctggtcgg cgcggcggca ggcgcgttta tcggcaacgc gctggcaaac
     5941 aaattcacac gggcaggcaa ccaagacagt cccgtcgccc ggcgcgcgcg tgccgcctac
     6001 catcagtccg cacatcccaa tgcgcgcacc agcagggatt tgaacacgcg cagcctccgt
     6061 gcaaaacaac aggcggcgca ggcgcagcgt taccgcccga caacgcgccc gcccgccaat
     6121 taccgccgcc ccgccatgcg cggtttcggc agaaggcggt aaatccggcg tgccaatgcc
     6181 gtctgaaggg ctttcagacg gcatttttgt atttgttagg gacattgtta tgttgccgtt
     6241 tgatttttag acggcatttt gtttccaaga gtttgatgtc gggatggcaa ttctgattta
     6301 cgtgcgttgg cagggcggca ttgtcatgcc gccgttgggt tttcagacgg catcatatcg
     6361 aaccgtcagg tcgtggggtg agcattcttg cccaccgttg caagggcggg attttttggt
     6421 gaacaggaat gcccaccctg catctgaaac ccttctgcgt cattcccacg caggaaggaa
     6481 tttagatatt tgaatttaca gaaattttca atcgttgcca aagcggaaaa gg
//
LOCUS       DAWKOG010000111         6528 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.111, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000111 DAWKOG010000000
VERSION     DAWKOG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6528
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.111"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(216..899)
                     /locus_tag="WJK66_001566"
     CDS             complement(216..899)
                     /locus_tag="WJK66_001566"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEZ7579261.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGRLQGLDKKQTAE
                     QYGDEQVRIWRRSYDTLPPLLDKDDAFSAHKDRRYAHLPADVIPDGENLQVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            1195..3498
                     /gene="parC"
                     /locus_tag="WJK66_001567"
     CDS             1195..3498
                     /gene="parC"
                     /locus_tag="WJK66_001567"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEZ7579262.1"
                     /translation="MNMQPHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKTDLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIIKEMQADMKQFGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSLGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSG
                     SGGYGFIAKLADMVGRVKAGKVVMTVDSGETVLPPVAVYASSLINPDCKIIAATNQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSEPEILIESEGRRGAAHKDRIPV
                     SLLEAKRGKKGKLLPISGSLKQLSSPK"
     gene            3518..5110
                     /locus_tag="WJK66_001568"
     CDS             3518..5110
                     /locus_tag="WJK66_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEZ7579263.1"
                     /translation="MVISNTRELEKLKDRIPNLINIARIAIVLPLLIMHIFGLEVSGH
                     ENLHASWTTWAFYLWLAIACWLIFFSTLNPQWQWQALRIPSFSAVADITMIGVLTYLF
                     GGIDSGFGILILPFVGSSCLLSYGRYPLLYASYASILLIFNALADSNINMYPLILDAK
                     TVTNTFVLVTGSYFVAMIASLSVRYIDRAGKLAHENHAAYRRIRGLNQIVLNRVQEAV
                     VVINVEHQTILFNKKAKDLLPMLEIGQHTALFDPVAVLWDKTSSRTFERNIDTPALTA
                     RIRAVPMNKEQNKLLILYIRPQSEIQAEALSVKLAALGQLTANLAHEIRNPMSAIRHA
                     NDLLRENAEEEETDPFKVKLCEIIDGNVRRIDKMLEDISSLNKRNKTEREAIDLMQFW
                     IGFKQEFLLNNPDAVGCIRLDMQGNHLTAYFDSAHLRQIMWNLCNNAWRHSSKRNGSI
                     SVTIRPAQKNTISILFADDGGGVPPEVQEHLFEPFYTTAKNGTGLGLYVARELAHANF
                     GDLTYLPEAKCFELALPEKSND"
     gene            5103..5570
                     /locus_tag="WJK66_001569"
     CDS             5103..5570
                     /locus_tag="WJK66_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEZ7579264.1"
                     /translation="MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVTEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG"
     gene            5674..6426
                     /locus_tag="WJK66_001570"
     CDS             5674..6426
                     /locus_tag="WJK66_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ7579265.1"
                     /translation="MTDILIDNTATETVRTLIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLAVTAFEQHPAVACLLSDGIRRALLNPETQDTAARITLHFGNAAVQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMTYFHRLVGEAQDEAALLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGTDKG"
BASE COUNT         1610 a         1987 c         1620 g         1311 t
ORIGIN      
        1 tcagaggttc tagattcccg cctgcgcggg aatgacggcg gagcggtttc tgcttttccg
       61 ataaatgacc gcaacctaaa acccatcctt cccacgaaaa cagaaaaaca aaaacctaaa
      121 atcccgtcgt tctcgcgaaa gcagttgcta cgtttaccgt caaaaatgcc gtctgaacgc
      181 tcaagccttc agacggcatt ttttatttca cacccttaca ggtagaattt ttcgatgact
      241 ttcaaattgt cgtccaattt gtacaccaac ggctgaccgg tcgggatttc caagcccatg
      301 atgtcttcgt cggaaatgcc ctcgatgtgt tttgccagcg cgcgcaggga gttgccgtgc
      361 gccgccacca agacgcgttt gccgctcaaa atcgcggggg cgatttggtc ttcccaaaac
      421 ggcaatacgc gctccagcgt tacttgcagg ttttcgccgt cggggataac atcggcaggc
      481 agatgggcat agcggcggtc tttgtgtgcg gaaaacgcgt cgtctttgtc caaaagcggc
      541 ggcagggtgt cgtagctgcg ccgccagatg cggacttgct cgtcgccgta ttgttcggcg
      601 gtttgttttt tgtccaggcc ttgcagtcgg ccgtagtggc gttcgttcag ccgccatgtt
      661 ttgatttgcg gtacgaacag ttggtcggat tcttccaaaa cgatgttgca ggtcttaatc
      721 gcgcgggtca ggacggatgt gaaggcgatg tcgaactcat agccgttttc tttcagtttt
      781 ttaccggcgg cagcagcctc ggcaagcccc tgctcgctca gcttcacgtc gcgccagcct
      841 gtaaacaggt ttttcgcgtt ccattcgctt tgtccgtggc ggataaatac caattccata
      901 tcgtctccaa tgtgtgaaag tgggaaagcc ttatttataa catattttca cattcccgta
      961 tttgattcag attcagacac gcgcccacta tggtttgccg ttttgattta caataatgcc
     1021 ctttgcttta cattccgcat acacaatgtt tcaacacaca ggacgacaca taaagcaccg
     1081 ccctatgtgt tgccctgatt tggaaggggt tgccccctcc caaataaagt ctgattctac
     1141 cgccccgaag gacagatgtc caagtggcgg gggttcaacc gaaaaggaaa tacgataaat
     1201 atgcaaccgc acgctccaca taccgattcc aatacgctga tgctcggccg atacgccgaa
     1261 cgcgcctatc tcgaatacgc catgagcgtg gtcaaaggcc gcgcgctgcc tgaagtttca
     1321 gacggccaaa agcccgttca gcggcgcatt ttgtttgcca tgcgcgatat gggtttgacg
     1381 gcgggggcga agccggtgaa atcggcgcgc gtggtcggcg agattttggg taaataccac
     1441 ccgcacggcg acagttccgc ctatgaggcg atggtgcgga tggcgcagga ttttaccttg
     1501 cgctatccct taatcgacgg catcggcaac ttcggttcgc gcgacggcga cggtgcggcg
     1561 gcgatgcgtt acaccgaagc tcggctgacg ccgattgcgg aattgctgtt gtccgaaatc
     1621 aatcagggaa cggtggattt cgtgccgaac tacgacggcg cgtttgacga gccgctgcac
     1681 ctgcccgccc gcctgcctat ggtgttgctc aacggcgcgt caggcatcgc ggtgggcatg
     1741 gcgaccgaga ttccgtcgca caatttgaac gaagtgacgc aggcagcgat tgcgctgttg
     1801 aagaagccga cgctggaaac cgccgacctg atgcaatata ttcctgcgcc cgattttgcc
     1861 ggcggcggtc aaatcatcac gccggcggac gaattgcgcc ggatttacga aaccggcaaa
     1921 ggcagcgtgc gcgtgcgtgc gcgttatgaa atcgagaagc tggcgcgcgg acagtggcgc
     1981 gtcatcgtaa ccgagctgcc gccgaacgcc aattccgcca aaatccttgc cgaaatcgaa
     2041 gaacaaacca acccgaaacc gaaagcgggt aagaaacagc tcaaccaaga ccagctcaac
     2101 accaaaaagc tgatgctgga tttaatcgac cgcgtgcgcg acgagtccga cggcgaacat
     2161 cccgtgcgac tggtgttcga accgaaatcc agccgcatcg ataccgatac cttcatcaac
     2221 acgctgatgg cgcaaacttc gctggaaggc aatgtgtcca tgaacttggt gatgatgggg
     2281 ctggacaacc gccccgcgca gaaaaacctg aaaacgattt tgcaggaatg gctggatttc
     2341 cgcaccgtaa ccgtaacacg ccgtctgaaa ttccgtttga accaagtgga aaaacggctg
     2401 cacatcctcg aaggccgtct gaaagtcttt ctgcacatcg acgaagtgat taaagtcatc
     2461 cgcgaatcag atgacccgaa aaccgatttg atggcggcgt tcgggctgac cgaaatccaa
     2521 gccgaagaca ttttggaaat ccgcctgcgt cagttggcgc gtttggaagg tttcaaactc
     2581 gaaaaagaat tgaacgaatt gcgcgaagaa caaggccgtc tgaacatcct tttgggcgac
     2641 gaaaacgaaa aacgcaagct gattatcaaa gagatgcagg cggacatgaa gcagtttggc
     2701 gacgcgcgcc gcacgctggt ggaagaggcc ggacgcgccg tgctgacaca aaccaccgcc
     2761 gacgaaccca tcacgctgat tctgtcggaa aaaggctgga tacgcagccg tgccggacac
     2821 aacctcgatt tgagccaaac cgcgttcaaa gaaggcgacc gcctcaaaca aactctcgaa
     2881 ggccgcactg ttttacccgt cgtcatcttg gattcattgg gcagaaccta cacgctcgat
     2941 gccgccgaaa tccccggcgg acgcggcgac ggcgtaccgg tttcatcctt aatcgagctg
     3001 caaaacggcg cgaaacccgt tgcgatgctg acaggattgc cggaacaaca ttatttatta
     3061 tcgggcagcg gcggctacgg cttcatcgcc aagttggctg atatggtcgg acgcgtgaaa
     3121 gcgggcaaag tggtcatgac cgtggacagt ggcgaaaccg tcctgccgcc ggttgccgtc
     3181 tatgcttctt cgctgatcaa cccagactgc aaaatcattg ccgccaccaa tcaaaaccgc
     3241 gccctcgcct tccccatcgg cgaattgaaa attatggcga aaggcaaagg actgcaaatc
     3301 atcggattaa acgccggcga atcgatgacg cataccgccg tttcttccga gccggaaatc
     3361 ctgattgaaa gcgaaggcag gcgcggcgcg gcgcacaaag accgcatccc tgtctccctg
     3421 cttgaggcaa aacgcggcaa aaaaggcaaa ctattaccca tatcgggcag cctgaaacag
     3481 ctttcttccc cgaaataaac ccgactctgc acatactatg gtaatttcca atacccgcga
     3541 acttgagaaa ctcaaagacc ggattcccaa tctgatcaac atcgcccgca tcgccatcgt
     3601 cctcccccta ctgattatgc atatcttcgg gttggaggtc agcgggcatg aaaacctgca
     3661 cgcctcatgg acgacatggg cgttttactt gtggcttgcc attgcctgct ggctgatttt
     3721 cttttccacc ctcaacccgc aatggcaatg gcaggctttg aggataccga gtttcagtgc
     3781 cgtggccgac atcacgatga tcggcgtgct gacctacctg ttcggcggca tcgattcggg
     3841 gttcggcatc ctgatcctac ccttcgtggg cagctcctgc ctactcagtt acgggcgtta
     3901 tcccctgctc tatgccagct atgcatccat cctgctgata ttcaacgccc ttgcagacag
     3961 caatatcaat atgtatccgc tgattctgga tgcaaaaacc gtcaccaaca ccttcgtcct
     4021 cgtgaccggc tcctatttcg tcgccatgat tgcctcgctg tcggtcagat acatcgaccg
     4081 tgccggcaaa cttgcccatg aaaaccacgc cgcctaccgc cgcatcaggg gcttgaacca
     4141 aatcgtgctc aaccgcgttc aggaagcggt cgtcgtcatc aacgtcgagc atcaaaccat
     4201 actgttcaat aaaaaggcaa aagacctgct acccatgctt gaaatcggac agcataccgc
     4261 cctgttcgac cctgtcgccg tcttatggga taaaacatct tcacgtactt tcgaacgcaa
     4321 tatcgacacg cccgccctga ccgcccgcat ccgcgccgtg ccgatgaaca aagagcaaaa
     4381 caagctgctc atcctctaca tccgcccgca aagcgaaatt caggcagaag ccctgtccgt
     4441 caaacttgcc gcactcggac aactgaccgc caacctcgcc cacgaaatcc gcaaccccat
     4501 gtccgccatt cgccatgcaa acgacctgtt gcgcgaaaat gcagaggaag aagaaaccga
     4561 cccattcaag gtcaaacttt gcgaaatcat cgacggcaac gtccgccgta tcgacaaaat
     4621 gctcgaagac atctcctcgc tcaacaaacg caacaaaacc gaacgcgaag ccatcgacct
     4681 gatgcagttt tggataggct tcaaacagga attcctgctc aataatcccg atgccgtcgg
     4741 ctgcatccgc ttggatatgc agggcaatca cctgactgcc tatttcgatt ccgcccactt
     4801 gaggcaaatt atgtggaacc tgtgcaacaa cgcatggcgg cacagcagca aacgtaacgg
     4861 ctcgatttcc gtcactatcc gccccgcgca aaaaaacacc atcagcatcc tgtttgccga
     4921 cgacggcggc ggcgttccgc ccgaagtgca ggaacacttg ttcgaaccct tttacaccac
     4981 agcaaaaaac ggaaccggct tgggactgta tgtcgcccgc gaactggcac acgccaactt
     5041 cggcgatttg acctacctgc ccgaagccaa atgtttcgaa ctcgcattac cggaaaaaag
     5101 caatgactga actgcaacat cccgttctcg tcgtcgatga cgaaaccgat attctcgacc
     5161 tgatggaaat gaccctgatg aaaatggggc tgcgcgtcca taccgcgtca ggcgttaccg
     5221 aagcaaaaaa caagctcgac agccaacgct attcgctcgt cctgaccgat atgcgtatgc
     5281 cggacggctc ggggctggaa gtcgtccaac acatcaacag ccgcctgctc gatacgccgg
     5341 ttgccgtcat caccgccttc ggcaacgccg atcaggcaca ggaagcgttg cgttgcggcg
     5401 cgttcgaccc cgataccatg cagatacagg actatctcga ccaaatcgaa cgcgacatta
     5461 tcgaacaaac cctcaaacaa accgaaggca accgcacgca ggccgccaaa cgcttgggca
     5521 tcagcttccg ttccatgcgc taccgtatgg aacgcctcaa catcggctga cgacaaaacg
     5581 gcatccgcac catctccgcc cacccgaaaa aatgccgtct gaaacggcac gggaaagcgg
     5641 gttcgcccca cgcccgaacg gacacaaaac accatgaccg acatccttat tgacaacacc
     5701 gccaccgaaa ccgtccgcac cctgatacgg gcattccccc ttgtgcccgt ttcccaaccg
     5761 cccgaacaag gcagttacct ccttgccgaa cacgataccg tcagcctcag gcttgtcggg
     5821 gaaaaaagca gcgtcatcgt cgattttgcc tccggcgcgg cacaataccg gcgcacaaaa
     5881 ggcgggggcg aactcatcgc caaagccgtc aaccacaccg cgcaccccac cgtttgggac
     5941 gcaaccgcag gattggggcg cgacagcttc gtcctcgcct cgctcgggct ggccgttacc
     6001 gccttcgagc aacatcccgc cgtcgcctgc ctgctttcag acggcatccg ccgcgccctc
     6061 ctcaatcccg aaacgcaaga caccgccgcg cgcatcaccc tgcatttcgg caacgccgca
     6121 gtacaaatgc ccgcacttgt tcaaacacaa ggcaaacccg acatcgtcta tctcgacccc
     6181 atgtatcccg aacgccgcaa aagtgccgcc gtcaaaaaag aaatgaccta cttccaccgg
     6241 ctcgtcggtg aagcgcaaga tgaggccgcc ctcctccata ccgcacgcca aacggcaaaa
     6301 aaacgcgtcg tcgtcaaacg cccccgcctc ggcgaacacc ttgccggaca agcccctgcc
     6361 taccaataca ccggcaaaag cacccgcttc gacgtttacc tgccctacgg gacggacaag
     6421 ggataacgcc cataaaacaa gacaccgaaa aatttgccgt tcttatgcaa acgagaaacc
     6481 ggtttttgcg tttcgactgt tttggataaa tcatcacacc ttaaagtt
//
LOCUS       DAWKOG010000112         6487 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.112, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000112 DAWKOG010000000
VERSION     DAWKOG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6487
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.112"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(110..1465)
                     /locus_tag="WJK66_001571"
     CDS             complement(110..1465)
                     /locus_tag="WJK66_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEZ7579266.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSLLENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAKPLKEGDHIEVSGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPTRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTPVQGAGSELQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIEACTVPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLAQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMKQNLRA
                     HTARFERQTQALQHYRPDVSVCKNNIVRLQTALPAAFSQLLTHRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHITFADGETDVRVSKEQGQQDLFDC
                     I"
     gene            1662..2483
                     /gene="nadE"
                     /locus_tag="WJK66_001572"
     CDS             1662..2483
                     /gene="nadE"
                     /locus_tag="WJK66_001572"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEZ7579267.1"
                     /translation="MMRIFSTGGKMDTQAVITHIVRWLDEYAARANAKGFVVGVSGGI
                     DSAVVSALAARTGRTTLLLDMPIRQHPDQLERARLHIRNLQRQYANVSAQTVDLTDTF
                     QTFEQTVGVHQTAFDNQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            complement(2553..3026)
                     /locus_tag="WJK66_001573"
     CDS             complement(2553..3026)
                     /locus_tag="WJK66_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ7579268.1"
                     /translation="MTDFSVWETAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAELLAYMQNELLMHMMKEERMLFPMINQGVGRGAAMPISVMMHEHEEHD
                     RAIARLKELTDNFQAPEGACGSWTRLYALAKEMVEDLNDHIHLENDILFARVLDS"
     gene            3329..3661
                     /gene="trxA"
                     /locus_tag="WJK66_001574"
     CDS             3329..3661
                     /gene="trxA"
                     /locus_tag="WJK66_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEZ7579269.1"
                     /translation="MSSELIVHTGDAAFEQDVLKSDLPVLLDFWASWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGDVVATKVGALAKGQLT
                     AFVEASIA"
     gene            complement(3714..4661)
                     /locus_tag="WJK66_001575"
     CDS             complement(3714..4661)
                     /locus_tag="WJK66_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ7579270.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAV
                     DVYGDQIHLQEYDTGWLMRSEEYEAWLAEVLEAVAFVTGFAPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWARRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRQFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            4989..6377
                     /locus_tag="WJK66_001576"
     CDS             4989..6377
                     /locus_tag="WJK66_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ7579271.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQRLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFARLEVLILDEAD
                     RMLDMGFIDDIETIVEATPEDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDADIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSAGGKPKG
                     KGGWGDRKSGGWRGDHKPSKEGFGGKTRGEGFKKEGFKRDGFKKTGEGFKGKRKAGDS
                     FAGKGERRYKDR"
BASE COUNT         1542 a         1749 c         1712 g         1484 t
ORIGIN      
        1 cggttttttg aatttcagtc atttctgata aaccccggcg gctttccgct tccggactcc
       61 cgcttttgcg ggaatgacgg cggaggggga gcggtttttc cgattgggtt taaatgcaat
      121 cgaacaaatc ctgctgccct tgttctttgc ttacgcgcac gtcggtttcg ccgtcggcga
      181 aggtaatgtg cagcttctgc ccctgcttca aaacatcggc gttgcggatg acttgtccgc
      241 gtgtgttttt gacgacggaa aagccgcgct ccagaatgtg ctgcggcgaa acggcttcga
      301 gcaatgcggc ttgggcggtc aggctttggc ggcggtgggt aagcagttgg ctgaaggcgg
      361 cgggcaatgc cgtctgaagg cggacgatgt tgtttttgca aacggaaaca tcaggacggt
      421 aatgttgcag ggcttgggtt tggcgttcaa aacgggcggt gtgggcacgg aggttttgct
      481 tcatcgagta agacagcgtt tgcgccagct tgccgattga agcgcgctgc tcatcaagtt
      541 tttggcgcgg atgacggatt tgccgcgcaa gccagtcgag tttttggctg gcatcgaaat
      601 agcgttgttc caaaacggtt ttcagacggc cttgcgcctg agcgagacga tgtagcgatt
      661 cttggcggtt ggggctgacc agctccgccg caccggtcgg cgtgggcgcg cgcacgtcgg
      721 cgacaaaatc ggcaagcgta aaatcggttt cgtgtcccac gccactgacg acgggaaccg
      781 tgcaggcttc gattgcgcgc acgaccggtt cttcgttaaa cgcccacaag tcttcaatgc
      841 tgccgccgcc gcgacagaca atcaacacgt cgcattcggc gcgttgcgag gcggttttaa
      901 tcgcttgggc aatttgtaac tcgctgcctg cgccttgaac aggtgtcgga taaacgataa
      961 cgggaatttc gggcgcgcgg cgtttcaagg tggtaacgac atcgcgcaat gccgctgctg
     1021 ccaggctggt gacgatgccg atacattgcg gacgggtagg caacggcttt ttgcgttccg
     1081 ccgcaaacgc gccttccgcc tgcaactgtg ccttcaaccg ctcgtaggct tcgtaaagct
     1141 gccccaaacc tttgagccgc acctcattca cggtaatttg aaattcgccc cgcgcctcgt
     1201 aaatactgat ttttcctgat acttcgatat ggtcgccttc tttcaaaggc ttcgccaaac
     1261 gcgccgccgc acctttgaac atcgcgcaac gcacctgcgc gcggctgtct ttgagcgaga
     1321 aataataatg tccgctggcg gcacgggtca ggttggacac ttcgcccgca atccacaggc
     1381 cggcaaggtg gttttccaaa agacttttgg caaatgcgtt caactcagaa acggacaaca
     1441 cgtcagaatg aaaaaaatca gacatagaat caatcaaata ataaaaaata tgaatatgtt
     1501 ttgaagccta aggcggtatt agtccgccta aattgtcaac aatattataa cacgcgccat
     1561 cttgccgccc gccttttccc gtatgacttt tttaagcggg gaatgggaaa aatattcatc
     1621 aacccgccct caatctattc aaattacacc gccgacaagc tatgatgcgg atattttcga
     1681 caggaggaaa aatggatacg caggcagtta tcacacatat tgtccgatgg ttggacgaat
     1741 acgccgcccg ggcaaatgca aaagggtttg tcgtgggcgt ttccggcggc atcgattccg
     1801 ccgtcgtctc cgcactcgcc gcccgcaccg gccgcaccac gctgcttctg gatatgccga
     1861 tacgccaaca ccccgaccag cttgagcggg caaggctgca catccgcaat ctgcaacggc
     1921 aatatgccaa tgtaagcgca caaacggtcg atctgaccga caccttccag acctttgaac
     1981 aaaccgtcgg cgttcatcag acggcatttg acaatcagcc gctttccctc gccaacgcca
     2041 gaagccgcct gcgtatgctg accctgtact actacgggca gatacacgga ctactggtta
     2101 cggggacagg taacaaaatt gaagatttcg gtgtaggatt ttttaccaaa tacggcgacg
     2161 gcggcgtgga catcagcccg attgccgacc tgaccaaaac ccaggtttac cggcttgccg
     2221 aagcattggg cgtggacgag gcgattcaaa aagccccgcc gaccgacggc ctgtgggata
     2281 cggaacgcac cgacgaagaa cagatgggcg caagctatcc cgaactggag tgggcaatgg
     2341 gcgtgtacgg cacgcgcaaa cccgaagatt ttgaagggcg gcagcgcgaa gttctggaaa
     2401 tctatacgcg gctgcaccgc gcgatgcagc acaaaatcaa cccgattccc gtgtgccgca
     2461 ttccgcccga attgttgggc tgaaacgtgg aaatgccgtc tgaagcggaa aaccgtgttt
     2521 cagacggcat tgacatatcc ggctcctatc ccttaagaat cgagtacgcg ggcaaacaaa
     2581 atatcgtttt ccaaatgaat gtggtcgttc aaatcctcca ccatttcttt ggccaaagcg
     2641 taaagccgcg tccagcttcc gcaggctcct tcgggcgctt ggaaattatc ggtcagctct
     2701 ttgagccttg cgatggcgcg gtcgtgttct tcatgttcgt gcatcatcac gctgatgggc
     2761 atcgccgcac cccgaccgac accctgatta atcatcggaa acagcatcct ttcctctttc
     2821 atcatatgca tcagcagttc gttctgcata taggcaagca gctcggcaat ttccgccgga
     2881 aaggtatcgg catgaacttg ggccactttt tgcgccagcg gcaccaattc ttcaaattgt
     2941 gcacggtgta cattgtggta gcgttgcagg atatgatcga cggttgcacc aaagggagca
     3001 gtttcccaaa cggaaaaatc agtcatggca gggttccttt tatttatagg gtttcgggtt
     3061 tggttttgaa cattcatact ttaagaatct atttaaacgg agcatacacc gcccgtgcgt
     3121 ttctgtacag cttcaaaaac attccttaca ttttgataat aaaagtaatt ttcaaaaata
     3181 aaatactgtc cgaaccgttt ttttagaatt tgcaaaggcg attggggcgg tacagaaaaa
     3241 ctattatccc gcccgcccac ttgaaatttt tatgcccaag ccctatcctg cacgctatcg
     3301 tgccaatccc aaccgaaaag gaaaaataat gagcagcgaa ttgatcgtac acaccggcga
     3361 tgccgccttc gagcaagatg ttttgaaatc cgacctcccc gtcctgctgg acttttgggc
     3421 ttcgtggtgc ggcccgtgca aaatgattgc cccgattttg gacgacattg ccgccgaatt
     3481 tgaaggccgt ctgaaagtag tcaaaatcaa catcgacgac aacgaagcca ccccgtcccg
     3541 tttcggcgtg cgcggcattc cgaccctgat ggtgttcaaa aacggcgacg tcgtcgccac
     3601 caaagtcggc gcattggcaa aaggtcagct gaccgccttt gtcgaagcct ctatcgcctg
     3661 ataaagcgca atcgaaaaag ccgccggaag attccggcgg ctttttcgca cccttaagat
     3721 ttgtggcgga tttcccagca ccgatggatt tttttgttgc ggaaatcttc gggaacggat
     3781 tgtttggaaa tgtctttgac ggcgtattgt tccgatacca agtcgtctaa gacgaagctg
     3841 cgcaggttgt tggaaaagta caaaatgccg tctgaagcga gcagcttcac cgcgccgtca
     3901 atcagctttt tgtgatcgcg ctgaatgtcg aggatgtcgg acattttctt gctgttggaa
     3961 aaactgggcg gatccatcac aatgaggtcg aactgcctgc cttccccata tgccgtctga
     4021 agatattgga acacgtcggc gcggacgatt ttgtgccgtt ccgtatcgat gccgttcaat
     4081 tcaaaattgc gtctcgccca atcgagataa gtgttggaca aatcgacagt ttcgctggat
     4141 gccgcgccgc cggtggcggc atagacagtg aagctgccgg tgtaggaaaa caggtttaaa
     4201 aaacgtttgc ccgccgccgt ttcgccgact tttttgcgcg tgtttcgatg atccaaaaaa
     4261 agccccgtat ccaaatactt atcaaggttg acccaaaact tgcggccgtt ttcggtgatg
     4321 acgaaatcgt cgcccgcctt gccggttttt tcgtattgct gcaaaccttt ttggcgttcg
     4381 cggcgtttga ggcggatttg ttcgggcgca aaaccggtaa cgaaggcgac ggcttccaag
     4441 acttcggcaa gccacgcttc gtattcttcg gaccgcatca gccagccggt atcgtattcc
     4501 tgaaggtgga tttggtcgcc gtaaacatcg acagcaaagg ggaattgggg gatgtcgcgg
     4561 tcgtaaatgc gccaggcttc gatgccgttg cgtttcgccc atttcataag gtgtttgatg
     4621 tttttgccca agcggttggc aaacggtgtg atgtcggtca ttggtttcag gcggaataaa
     4681 gtggaaaacg gcaattttac tgtaattaac gcccgattgc ttgaccgttt cgggcaaacc
     4741 ctataccatc cgtcgcttat cttgtcatac gaagccatcg ccttccaacc taaaccgccc
     4801 ttacgggcgc gtttcttctg ttgctttgat tttgcaaagc gtatctgtgc aggttgccgt
     4861 cgatgtaaac cacaagcaag ccgcttgcga caaccctgta acttcacatt ccccgtatcg
     4921 ttacccttcc ctgcttcagg ccgtctgaac ctttcggacg cgagcgttgt tgtcttccaa
     4981 ggatagccat gtctattaaa tttgccgatt tgaaccttga taaaaacatt ttgtccgccg
     5041 tcagcagcga gggttacgaa agcccgacgc cgattcaggc gcaagccatt ccgtttgctt
     5101 tggaaggccg cgacatcatg gcttcggcgc aaaccggctc cggcaaaacc gccgcctttc
     5161 tgttaccgac tttgcagcga ctgaccaaac gcagcgaaaa accgggcaaa ggcccgcgcg
     5221 cgttggtgtt gacgccgacc cgcgaattgg cggctcaagt cgagaaaaac gcgctggcgt
     5281 atgccaaaaa tatgcgttgg ttccgtaccg tcagcatcgt cggtggtgca tcgttcggct
     5341 accaaacccg cgccctgagc aaaccggtcg atctgattgt cgccacgccg ggccgtctga
     5401 tggacttgat gcaaagcggc aaagtcgatt tcgcccgctt ggaagtgctg attttggacg
     5461 aagccgaccg tatgttggat atgggcttta tcgacgacat cgaaaccatc gtcgaagcca
     5521 cgccggaaga ccgtcagact ttgttgttct ccgccacttg ggacggcgcg gtcggcaaac
     5581 tggcgcgcaa actgaccaaa gaccctgaaa tcatcgaagt cgaacgcgtg gacgaccaag
     5641 gcaaaatcga agaacaactg ctgtactgcg acgatatgcg ccacaaaaac cgcctgctcg
     5701 atcatatctt gcgcgatgcc gacatcgatc aatgcgtgat tttcacgtcc accaaagcca
     5761 tgaccgaagt catcgcggat gaattgtacg aaaaaggttt cgccgccaac tgcctgcacg
     5821 gcgatatgcc gcaaggctgg cgcaaccgca cgctgatgga tttgcgcaaa ggccgctgca
     5881 aaattttggt tgccaccgac gttgccgcac gcggtatcga cgtaccgacc attacccacg
     5941 ttatcaacta cgacctgccg aaacaggcgg aagactacgt ccaccgcatc gggcgcaccg
     6001 gccgcgcagg ccgcacgggt attgcgatta cgtttgccga agtgaacgaa tacgtcaaag
     6061 tccacaaaat cgaaaaatac attaaccgaa aactgcccga actgaccatc gaaggcatgg
     6121 aaccgacccg caaacgcaaa tccgcaggcg gcaagccgaa aggcaaaggc ggctggggcg
     6181 atcgtaaatc cggcggttgg cgcggcgatc ataaaccgag caaagaaggc ttcggcggca
     6241 aaacgcgcgg cgaaggtttc aagaaagaag gctttaagag agacggtttc aaaaaaaccg
     6301 gcgaaggctt caaaggcaaa cgcaaagccg gcgattcttt tgcaggcaaa ggcgaacgcc
     6361 gttacaaaga ccgctaagcc ccaacctgcc gcataaacca atgccgtctg aaaccgattt
     6421 cgagtttcag acggcatttt tgcaatgttt cagcaccgcc cggctttgat acccaaagga
     6481 ttaatct
//
LOCUS       DAWKOG010000113         6480 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.113, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000113 DAWKOG010000000
VERSION     DAWKOG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6480
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.113"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            95..1173
                     /gene="hupA"
                     /locus_tag="WJK66_001577"
                     /pseudo
     CDS             95..1173
                     /gene="hupA"
                     /locus_tag="WJK66_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225846.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupA"
     gene            1204..3636
                     /gene="hupB"
                     /locus_tag="WJK66_001578"
     CDS             1204..3636
                     /gene="hupB"
                     /locus_tag="WJK66_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HEZ7579272.1"
                     /translation="MPIPFKPVLAAAAIAQAFPAFAADPAPQSAQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLVNDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGDIEIKNDGYVYNPTDTGGPSKYLTYVATGVARSQPDPQEWVNKSTLFKL
                     GYNFNDQNRIGWIFEDSRTDRFTNELSNLWTGTTTSAATGDYRHRQDVSYRRRSGVEY
                     KNELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDLRGINGEVYHSFRHIRQNTAQWT
                     ADFEKQLDFSKAVWAAQYGLGGGRGDNANSDYSYFAKLYAPKILASNQAKITMLIENR
                     SKYKFAYWNNAFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTTAIRGQIPHLGSERAH
                     AGFSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKANPNLKAEKAK
                     NWELGLAGSGKAGNFKLSGFKTKYRDFIELTYMGVSSDDKNNPRYAPLSDGTALVSSP
                     VWQNQNRSAAWVKGIEFNGTWNLDSIGLPKGLHTGLNVSYIKGKATQNNGKETPINAL
                     SPWTAVYSLGYDAPSKRWGINAYATRTAAKKPSDTVHSNDDLNNPWPYAKHSKAYTLF
                     DLSAYLNIGKQVTLRAAAYNITNKQYYTWESLRSIREFGTVNRVDNKTHAGIQRFTSP
                     GRSYNFTIEAKF"
     gene            complement(4224..5858)
                     /gene="groL"
                     /locus_tag="WJK66_001579"
     CDS             complement(4224..5858)
                     /gene="groL"
                     /locus_tag="WJK66_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEZ7579273.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVDELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAALDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLDDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEEKPAMPDMGGMGGMGGMM"
     gene            complement(5951..6241)
                     /locus_tag="WJK66_001580"
     CDS             complement(5951..6241)
                     /locus_tag="WJK66_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEZ7579274.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
BASE COUNT         1581 a         1909 c         1572 g         1418 t
ORIGIN      
        1 tttttgcacc gacccacaaa accagttgac atcatataac atttaaactg ataatcaaaa
       61 ctattattaa cacatcatca agatcgagta tgcgatgaaa tacaaatccc tgtccttact
      121 gccgcttgcc gccgcccttg ccgcctgtgc cggggggggg gggtagccga accgcacgtc
      181 cccgtgtcca tccccaccgc cacgccgctg cccaaaggcg aggtaacgtt atcaagcgat
      241 aacggcaata tcgaaaacat caacaccgcc aacactgaaa cacatccgcc gcgccgcacc
      301 agacgctccc tctatgcctc cccgcaaaac acatcctccg gtatttccat tcagcagcgg
      361 gaagtagaaa aaatctattt cggtgttaaa tcaccagaaa aatcattcat cttccaaaca
      421 cccggcggcg cgcaatacgc cctttcatcc tacgccgacc ccatcgtccc cagctactcc
      481 tcccccgact tcaaaattcc cgaccgccac gcagggcagc ggcttgccga cggcagccgc
      541 atctttatct gctgcagcga ctccggggct acttcgtatg cggaaattac caaacaagat
      601 tatatgaaat tcggggcgtg gataggcccc aacggcgaaa tcgacctctt cgccggcggc
      661 ttccccatcg gcaaaacgcc gcctcctgcg ttctcctggg gcagccccac gaccgaaacc
      721 gcattgtcaa aaggcaaaat cacttatcag gtttggggca tccgcgtcag aaacgggcaa
      781 tttgttactt cctcttatac gccgcccaaa ggcagctctt ggagtggtta tcaaaacacc
      841 cccgtccttt cctttatcac cgccaatttc aacagcaaca aactggcagg cgaaatcatc
      901 ggcaacagcg attacgggcc aaatgtgaaa attgaaaacg caacgattga cggactatcc
      961 ttttccggcg atgccacctc caacggcaaa accggcaact tggaaggcaa gttttttggc
     1021 aagttcaaca gcacccgcag cagcgacacc ggcataggcg gcaaaatcac ttttaaagac
     1081 gaccgctccc tcgataccgt attcggcggc gtgatttatg agaaaaaact tgatgacaaa
     1141 accagtcaag ataccaacca tctcaaaaaa taataaccca tccgctttta aaaaggtttt
     1201 cttatgccca tcccctttaa acccgtattg gctgccgccg ccatcgccca agcgtttccc
     1261 gcctttgcgg cagaccccgc gccgcagtcc gcccaaacgc tgaacgaaat caccgttacc
     1321 ggcacgcaca aaacccaaaa actcggcgaa gaaaaaatcc gccgcaaaac tttagacaag
     1381 ctcttggtca acgacgaaca cgacctggtg cgctacgacc ccggcatttc cgtcgtcgaa
     1441 ggcggcaggg cgggttctaa cggctttacc atacgcggcg tggacaaaga ccgcgtcgcc
     1501 atcaacgttg acgggctggc gcaggcggaa agccgctctt ccgaagcctt ccaagaattg
     1561 ttcggcgcgt acggcaactt caacgccaac cgcaacactt ccgagccgga aaacttttcc
     1621 gaagtaacca tcaccaaagg cgcggactcg ctcaaatccg gcagcggcgc attgggcggc
     1681 gcagtcaatt accaaaccaa atccgcaagc gattatgttt ccgaagacaa gccctaccat
     1741 ttggggataa agggcggcag cgtcggcaaa aacagccaaa aattcagcag catcaccgcc
     1801 gccggcaggc tctttggttt ggatgcctta ttggtttata cccgccgctt cggcaaagaa
     1861 accaaaaacc gctcgaccga gggcgatatc gaaattaaaa acgacggata tgtctataac
     1921 ccgaccgata caggcggacc cagcaagtac ctgacctatg tagccacagg ggttgcgcgc
     1981 tcccaacccg acccgcaaga atgggtaaac aaaagcaccc tgttcaagct gggctacaac
     2041 ttcaacgatc aaaaccgtat cggctggatt tttgaagact cgcgcaccga ccgttttacc
     2101 aacgagctgt ctaatttgtg gacgggtacg accacgtctg ccgcaacggg cgactaccgc
     2161 caccgccaag acgtgagcta ccgccgccgc tccggtgtcg aatacaaaaa cgaattggaa
     2221 cacggcccgt gggacagcct caagctgcgc tacgacaagc agcgcatcga tatgaacact
     2281 tggacttggg acatcccgaa aaattacgat ttgcgcggca tcaacggcga ggtttaccat
     2341 tcgttccggc atatccgcca aaacaccgcg caatggactg ccgattttga aaaacaactc
     2401 gacttttcca aagccgtttg ggcggcgcaa tacggcttgg gcggcggcag aggggacaat
     2461 gccaactcgg attacagcta tttcgcaaaa ctgtacgccc ccaaaatcct cgcttccaac
     2521 caagccaaaa tcacaatgct gatcgaaaac cggtcgaaat ataaatttgc ctattggaac
     2581 aatgcgtttc acttgggcgg caacgaccgc ttccgcctga acgcgggcat acgctacgac
     2641 aaaaacagca gcagcgcgaa agacgatccg aaatacacca ccgccatccg ggggcagatt
     2701 ccccatttgg gttcggaacg cgcgcacgcg ggcttcagct acggcacggg gttcgactgg
     2761 cggtttacca agcatctgca cttgttggca aaatacagca ccggcttccg cgcaccgact
     2821 tcggacgaaa cctggctgct gttcccacac cccgatttct acttgaaagc caacccaaac
     2881 ctgaaagcgg aaaaagccaa aaactgggaa ttgggtctgg cgggcagcgg caaagcgggc
     2941 aacttcaagc tctcgggctt caaaaccaaa taccgcgact ttatcgaatt gacgtatatg
     3001 ggcgtttcgt cagacgataa aaacaacccc agatacgccc cgctttcaga cggtacggca
     3061 ttggtcagct cgcccgtttg gcaaaaccaa aaccgctccg ccgcctgggt gaaaggcata
     3121 gagtttaacg gcacgtggaa cctcgacagt atcggtttgc ccaaagggct gcacaccggc
     3181 ctcaacgtca gctacatcaa gggcaaggca acgcaaaaca acggcaaaga aacgcccatc
     3241 aacgcgcttt cgccgtggac ggcggtttac agcctgggct acgacgctcc ttccaaacgt
     3301 tggggcatca acgcctacgc cacgcgcacc gccgccaaaa agccgtccga caccgtccac
     3361 agcaacgacg acttgaacaa cccgtggcct tatgccaaac acagcaaggc ctatacgctg
     3421 ttcgaccttt ccgcctacct caacatcggc aaacaggtta cgctccgcgc cgccgcgtac
     3481 aacattacca acaagcagta ctacacttgg gaatctttac gcagcatccg cgagttcggc
     3541 acggtcaacc gcgttgacaa caaaacccac gccggcatcc aacgctttac ctcgccgggc
     3601 aggagctaca atttcaccat cgaagcgaag ttctaagctc ctgatgtgcc aatgccgtct
     3661 gaaacccgat gatttgggtt tcagacggca ttttttacgg tcgcaccgcc gccccctttc
     3721 aggcttgtac caagcccaat cccgcaactc tccgtcattc ccgcaactct ccgtcattcc
     3781 cacggaagtg ggaatctaga aacgcaaagc tgcaagaatt tatcggaaat gactgaaact
     3841 caacgaacct ggattcccgc ctgcgcggga atgacgaagc tatccgcaca gaaacctgca
     3901 ccacgtcatt cccacgaaag tgggaatcca gaacgtaaaa tctgaagaaa ccgttttatc
     3961 cgatacgttt ccgcaccgac agacctagat tcccgctttc gcgggaatga cggcggaaag
     4021 gttgctgttt ttccgataaa ttcctgccgc tcttcgtttt tgggatggcg ggaaataaaa
     4081 caaaagcgcg cgtatcaaaa aacaacaatg caaagaacgg tgttggcaat tttaaggttt
     4141 gcgccttagc cgcacttatt cgcaaaaaaa ccgcacggcg ttgaccgtgc ggcttgttgt
     4201 ctgaaggctt cagacggcat tgcttacatc atgccgccca taccacccat gccgcccata
     4261 tcgggcatag caggtttttc ttcagggatt tcggcaatca tgcaatcggt cgtcagcatc
     4321 aggccggcga tggacgcggc gtgttgcagc gcggaacggg ttactttggc ggggtcgagt
     4381 acgcccattt cgatcatgtc gccgtattcg ccggagcctg cgttgtaacc gtagttgcct
     4441 ttgccttcca acactttgtt caccaccacg ctaggttcgc cgcctgcgtt ggcaacgatt
     4501 tggcgcagcg gagactcaac ggcgcgcaag acgatttgta cgcctgcgtc ttggtcggca
     4561 ttgccggtgt gcaggttttc cagagcggca cgggcgcgca acagggcgac accgccgcct
     4621 gcaaccacgc cttcttcaac ggctgcgcgg gtagcgtgca gcgcgtcttc cacgcggtct
     4681 tttttctctt tcatttcgac ttcggtcgcg gcaccgactt tgatcactgc cacgcctcct
     4741 gccaatttgg caacgcgctc ttgcagtttt tctttgtcgt aatcgctggt cgcggtttcg
     4801 atttgttggc ggatttcggc aacacgcgct tcgatttggg ctgcgtcgcc gaagccgtcg
     4861 atgatggtgg tgttttcttt accgatttcg atgcgtttgg cttgacccaa gtcgtccaaa
     4921 gtcgcttttt ccagagacag gccgacttct tcggaaatca ccacgccgcc ggtcaggatg
     4981 gcgatgtctt gcaacatcgc tttgcggcgg tcgccgaagc caggggcttt gacggcaacg
     5041 gttttcagga tgcctcggat gttgttcacg accaaagtcg ccaaggcttc gccttctacg
     5101 tcttcagcga taatcaacag cggacggctg gcttttgcca cttgttccaa aacaggcagc
     5161 aagtcgcgga tgttgctgat ttttttgtcg aacaacaata caaacggatt gtccaaagca
     5221 gcgatttgtt tttcagcgtc gttgatgaag taaggagaca agtagccgcg gtcgaactgc
     5281 ataccttcaa ccacgtccag ctcgttttcc aaagatttgc cgtcttcaac ggtaatcacg
     5341 ccttctttgc cgactttttc catcgcttcg gcgataatcg cgccgacttg ttcgtcagag
     5401 ttggcggaaa tcgagccgac ttgggcgatt tctttagaag tgtcgcaagg tttggcgatg
     5461 tttttcagct cgtcaaccaa agcggcaacg gctttgtcga taccgcgttt caggtcggtc
     5521 gggttcatac cggcggtaac gtatttcata ccttcggcaa cgatggattg cgccaatacg
     5581 gtggcggtag tcgtaccgtc gcccgccacg tcgttggttt tggacgcgac ttctttcacc
     5641 atttgcgcgc ccatattttc aaacttgtct ttcaattcga tttctttggc gacggttacg
     5701 ccgtctttgg tgatgtgcgg gccgccgaat gcgcggtcaa ccaccacgtt gcggcctttg
     5761 ggacccaagg ttacgcgtac ggcgtttgcc agaatgttca cgccgtttac cattttttga
     5821 cggacttcat tgccgaactg tacgtctttt gctgccattt caattctcca aaaatcatta
     5881 aaactgtctg ataaaaccgt ttatgccgtc tgaaggcggt ttgccgtttc agacggcatc
     5941 gtgtccgtat ttatttttca acgatgccga agatgtcttc ttcgcgcatc accaacagct
     6001 cttcgccgtc ggcttttacg gtttggccgc tgtatttgcc gaagatgatt ttgtcgccga
     6061 ctttgacatc cagcggacgg cgcgcgccgt ctttaccgat tttgcccgcg cccacggcga
     6121 tgacttcgcc catatcgggt ttttcggcgg ccgcacccgg caaaacgatg cccgatgcgg
     6181 ttttctcttc agcttccaag cgtttgacga cgacgcggtc gtgtaaagga cggatggtca
     6241 tatctatgct ccgataaaat ggtttgaaaa caatcatctg cccgaacggt tcggacagat
     6301 taaggatgaa aaccggacag ccgtcaggcg gctgcccgta taagtcggca aaattagggt
     6361 gtgtgcgggc aaattcaagt gaggcggaaa aaatttattt ccgccgtttt ttatagtgga
     6421 ctaaatttaa ggggctgtac tagattagca gatatgttac cctcggaata taaagataac
//
LOCUS       DAWKOG010000114         6405 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.114, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000114 DAWKOG010000000
VERSION     DAWKOG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6405
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.114"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..263)
                     /locus_tag="WJK66_001581"
     CDS             complement(<1..263)
                     /locus_tag="WJK66_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579275.1"
                     /translation="MFGGRLKNAPSITAKHFLKENIMKFINTCRKYGAKLAVVTAAPL
                     ALAAHANATLPDTAKNALEAAKADGMEVGWIVVGIFAALFVFS"
     gene            complement(271..468)
                     /locus_tag="WJK66_001582"
     CDS             complement(271..468)
                     /locus_tag="WJK66_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579276.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
     gene            complement(473..763)
                     /locus_tag="WJK66_001583"
     CDS             complement(473..763)
                     /locus_tag="WJK66_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579277.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGKSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQLVKKG"
     gene            complement(808..2150)
                     /locus_tag="WJK66_001584"
                     /pseudo
     CDS             complement(808..2150)
                     /locus_tag="WJK66_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            complement(2236..2451)
                     /locus_tag="WJK66_001585"
     CDS             complement(2236..2451)
                     /locus_tag="WJK66_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579278.1"
                     /translation="MAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAIL
                     CNKERLTSSEPLSDFVLSVLKPYILRP"
     gene            complement(2739..3092)
                     /locus_tag="WJK66_001586"
     CDS             complement(2739..3092)
                     /locus_tag="WJK66_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579279.1"
                     /translation="MFTTNDLRHFLEGLAILFSIGYWGTMLLLLWFLVRFAYKKPKRN
                     PGKILSGSLTAAAILMLFVWIIPKQFGPIKEEIQAQEEWDRKYKEAEAVFNEQCKTAG
                     ERFTRRRTMWKGLCC"
     gene            3328..4755
                     /locus_tag="WJK66_001587"
     CDS             3328..4755
                     /locus_tag="WJK66_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEZ7579280.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLEPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQSRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVVAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            4824..6227
                     /locus_tag="WJK66_001588"
     CDS             4824..6227
                     /locus_tag="WJK66_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEZ7579281.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTDLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
BASE COUNT         1594 a         1532 c         1630 g         1649 t
ORIGIN      
        1 atggaaaaga caaaaagcgc ggcgaaaatg cccactacaa tccaaccgac ttccataccg
       61 tcggcttttg cggcttccaa agcgtttttt gctgtatcgg gcaacgttgc atttgcatgt
      121 gcggccaaag ccaggggagc agctgttaca acagccagtt ttgcgccgta tttacggcag
      181 gtgttaataa atttcatgat attttccttc aaaaagtgtt tggcggtaat ggatggagcg
      241 tttttcagac gaccgccgaa catccgaaaa tcagtctttc aaaaatccga atacgacaaa
      301 ttcgtattgg ttgccgattt cttccaaacc tgcgttaatc gcttcttcga agtcgtagaa
      361 ataatcggca ttggtgatta atttggtatg tccgatgtcg cccgtttcag gagagagata
      421 cagaaagtcc cctgttgata cggactggac aacatagact ttctgcattc aatcagcctt
      481 tcttcacgag ttgaaaaccg atgactttca gtttttgggt tttgcccgta gtaacgattt
      541 ccacgttcag gtttgcttcg atgggaaatt gggcgtttcg gaactgctcg aaattggcag
      601 agccgccgaa atcgtattca gtagtagagc tgcccaatgc gttgccttgg gagctgtcta
      661 agggtgtggc gacaatcagg cggcaatagt cgaagctctt gccttcgatt tgtccgttga
      721 attttttaac gccgacgatg tggccttgaa gttggatgtt cattttttgg tttccttgtg
      781 tgattaaacg tctttcgggc agacacttta agcccatgaa atcggtagtc ttgcgaattt
      841 gtcgtaaatg aagttgttat agctttcttc attgttgacg tgtttttgct gttcaagctg
      901 tttttcaaga ttctcgtaat attcgtacat atagtaaggg tctttgtacg gtttgaatgc
      961 gggctgttca tgaatggctt gagctttcaa aaaggcgcag tcgtaggctt cgggagtcaa
     1021 agacttgggc agcttgtgat gactcggctc aatcagttca aacagtttgg ctttgtccaa
     1081 ttcgggaaaa atgaatttca gaccgtttgc cgcacgtccg aactgttttt tacccattca
     1141 aggtaacggt cggctgaaat gaccttatct tccttaaccg cgtgtatgcg cgttgccttt
     1201 tggacgaatc gttctcaaat cggatatgcg ccgccgaaat attcgcccgg attctgcaaa
     1261 acttcaaaag ggataacgat gtcttttgct ttgaattcaa tttcaaagcg cgtccatgtg
     1321 cttgttttat cgcccaactg cttgcctttt tcatagacgc ggacatattt ggacgattca
     1381 cgggagccga taccataggt cttgcctttg gtcattttgg cttcatcgtc ttcttcccaa
     1441 tctgacccca aacattcgcc ttttggtttg acgtgatgac aggtaaacat acctttattt
     1501 cggtcttcac gggcttggtt cgggctgtat tcgccgttga aaaagtcttt tgcgatgtca
     1561 acgcgtgtga tttttgggcg gattgcatta gtcaggaatg cgaaaagtcg tgattcccag
     1621 ccttcttttg cgacgccgca accggtgccg gtcagttcga aaagaatggt attgttttgt
     1681 ccgccaaaat ggacgcgacc gtatagggcg tcttccgaac ccatcaacca acagcgctca
     1741 taaaaacgcc cgcccgagcc tttggattct ttgtagatac cgaaaccgaa aacttcttcg
     1801 gcgagcattg acgcggcgcg aataaaatct tcgtcttcca aaagacttac acgaacgccg
     1861 tatttatcga aaaagggttt ttcatgaaat gaaaagctga tttggtcgat gaaagccgaa
     1921 tcggatacac cgcgcctaag aggaacgcct aacaggtttc ctttaccgtc cgttatgtac
     1981 atttcgtaac attcgaagac ttcctgaacc ctgcctgccg tttcggtttc tgtacccctc
     2041 ctgttagata aggggggaag atttgaagcg gttgccggct tcctgccgtc cgctaacgcg
     2101 tccgtcatca cgccgacaac cgcctttgtc atcccttgct tatcttccat ggtgcgaatc
     2161 ctcaaaaaca ggcaaaaaaa aagccctgtt acttgtagaa agtaaaggac gttaattttt
     2221 gttaatcgtc ccttcttagg gacgcaatat ataaggtttt aaaacgctaa gaacaaaatc
     2281 cgataacggc tctgaagaag taagtctttc tttattgcac aagatagcaa acttccacgg
     2341 cattctcgcc ccacccgtgc cgtccaagcc tttttcaaag acatagatgg ttttcttggc
     2401 aatcacttcg tcagtttggc ggtcgataat ccgtatggtc gcacctgcca cccagtgcct
     2461 gcgcagcttg gaatcgacat tgttttcgaa cgttaccgca taacgggcgg ggtgtatatg
     2521 attaaatatt tgatttatcg gaaaatcttt acctgaatac cgaataatat cggaatggtt
     2581 gggttgcagg acatccacat agcggtattc cccatcgcta aaaaatccta ggaaacgagc
     2641 aataaaattt acgccttcgc tggtctgtaa agccgcattg tcccacatcg ggtctctggt
     2701 ttttgcatct gccgaaacgg tacgctcagg tactaccttc aacagcataa tcccttccac
     2761 attgtccgcc gtctggtaaa tctttccccc gccgttttgc attgttcgtt aaacacggct
     2821 tcggcttctt tgtattttct gtcccactct tcttgtgcct gtatttcttc tttgatcggg
     2881 ccgaattgtt tgggaataat ccaaacaaac agcatcagga tagcggcggc ggtcaggctg
     2941 cctgaaagga ttttgccggg gttccgtttg ggctttttat aggcaaagcg gacgagaaac
     3001 caaagcaaca gcagcatggt gccccaatag ccgattgaga ataggatggc caaaccttct
     3061 aggaaatggc gtaaatcgtt tgtggtaaac atgggttgtt cctgtggtta aatgtgcagg
     3121 ctgctttttg ccgaaccttg ccgcatctca aaagcagcct gcgcttcagc gttgcgttac
     3181 gcagtaaaat aatgaatatt tgtaacgact tgggtatttt ttgtcaatat tcccgccttt
     3241 cccttaacag ctgccgcgct ttccgttaaa attcctttac atatttatat tgtttcctgt
     3301 ttctatattg ccaaggttat acccgttatg tttttctccg ccctgaaatc ctttctttct
     3361 cgatacatta ctgtatggcg caatgtttgg gcggtgcgcg accagttgga accgcccaaa
     3421 cgcacggcgg aagaacaggc gtttttgccc gcgcatttgg aactgaccga tacgccggtc
     3481 tctgccgctc cgaaatgggc ggcgcgtttt attatggcgt ttgcgctttt ggctttgttg
     3541 tggtcctggt tcggcaaaat cgatattgtg gcggcggctt cgggcaaaac ggtgtcgggc
     3601 gggcgcagca aaaccatcca gccgctggaa acggcggtgg ttaaggcggt acatgtgcgc
     3661 gacgggcagc atgtgaaaca gggagaaacg ctggcggaac tggaggctgt gggaacagac
     3721 agcgatgtgg tgcagtcgga gcaggctttg caggctgccc aattgtccaa actgcgttat
     3781 gaagcggtat tggcggcatt ggaaagccgt accgtgccgc atatcgatat ggcgcaagca
     3841 cggtctttag gtctctccga tgccgatgtg caatcggcgc aggtgttggc gcagcaccag
     3901 tatcaggcat gggcggcgca ggatgcgcaa ttgcagtcgg ctttgcgcgg ccatcaggcg
     3961 gaattgcagt cggccaaggc gcaggagcag aagctggttt cggtgggggc gatcgagcag
     4021 cagaaaacag cagactaccg ccgtttgcgg gccgacaatt ttatttcgga acatgcgttt
     4081 ttggagcagc agagcaaatc ggtcagcaat tggaacgatt tggaaagtac gcgcggtcag
     4141 atgaggcaga ttcaggcggc cattgcacag gcggagcaga gtcgggtgct gaatacgcag
     4201 aacctgaaac gcgatacgct ggatgcgctg cgccaggcaa acgaacagat tgaccaatac
     4261 cgcggccaaa cggataaggc aaagcagcgg cagcagctga tgacaataca gtcgcctgcg
     4321 gacggcacgg tgcaggaatt ggccacctat acggtgggcg gtgtggtgca ggctgcccaa
     4381 aaaatgatgg tggttgcgcc cgatgacgac aaaatggacg tggaagtttt ggtattgaac
     4441 aaagacatcg gttttgtgga acagggacag gatgcggtgg tgaagattga gagttttccc
     4501 tatacgcgct acggttatct gacgggcaag gtgaaaagtg tcagccatga tgcggtaagc
     4561 cacgaacagt tgggcttggt ttatacggcg gtggtgtcgc tggacaaaca taccttgaat
     4621 attgacggca aagcagtgaa tctgacggcg ggcatgaatg tcacggcgga gattaaaacg
     4681 ggtaaacggc gggtgctgga ttatctgtta agcccgctgc aaaccaaatt ggacgaaagc
     4741 tttagggagc gataggcgga tccgtactgg gcatttgtta tccgccggtt cggacatgca
     4801 gactgcctga aaccattgcc cggatgacat tgctcaatct aatgataatg caagattacg
     4861 gtatttccgt ttgcctgaca ctgacgccct atttgcaaca tgaactattt tcggctatga
     4921 aatcctattt ttccaaatat atcctacccg tttcactttt taccttgcca ctatcccttt
     4981 ccccatccgt ttcggctttt acgctgcctg aagcatggcg ggcggcgcag caacattcgg
     5041 ctgattttca agcgtcccat taccagcgtg atgcagtgcg cgcacggcaa caacaagcca
     5101 aggccgcatt ccttccccat gtatccgcca atgccagcta ccagcgccag ccgccatcga
     5161 tttcttccac ccgcgaaaca cagggatgga gcgtgcaggt gggacaaacc ttatttgacg
     5221 ctgccaaatt tgcacaatac cgccaaagca ggttcgatac gcaggctgca gaacagcgtt
     5281 tcgatgcggc acgcgaagaa ttgctgttga aagttgccga aagttatttc aacgttttac
     5341 tcagccgaga caccgttgcc gcccatgcgg cggaaaaaga ggcttatgcc cagcaggtaa
     5401 ggcaggcgca ggctttattc aataaaggtg ctgccaccgc gctggatatt cacgaagcca
     5461 aagccggtta cgacaatgcc ctggcccaag aaatcgccgt attggctgag aaacaaacct
     5521 atgaaaacca gttgaacgac tacaccgacc tggatagcaa acaaatcgag gccatagata
     5581 ccgccaacct gttggcacgc tatctgccca agctggaacg ttacagtctg gatgaatggc
     5641 agcgcattgc cttatccaac aatcatgaat accggatgca gcagcttgcc ctgcaaagca
     5701 gcggacaggc gcttcgggca gcacagaaca gccgctatcc caccgtttct gcccatgtcg
     5761 gctatcagaa taacctctac acttcatctg cgcagaataa tgactaccac tatcggggca
     5821 aagggatgag cgtcggcgta cagttgaatt tgccgcttta taccggcgga gaattgtcgg
     5881 gcaaaatcca tgaagccgaa gcgcaatacg gggccgccga agcacagctg accgcaaccg
     5941 agcggcacat caaactcgcc gtacgccagg cttataccga aagcggtgcg gcgcgttacc
     6001 aaatcatggc gcaagaacgg gttttggaaa gcagccgttt gaaactgaaa tcgaccgaaa
     6061 ccggccaaca atacggcatc cgcaaccggc tggaagtaat acgggcgcgg caggaagtcg
     6121 cccaagcaga acagaaactg gctcaagcac ggtataaatt catgctggct tatttgcgct
     6181 tggtgaaaga gagcgggtta gggttggaaa cggtatttgc ggaataaagc aggctgaaac
     6241 ggttatgaaa ttcccaaagc agcctgcacc ccgtttcgaa agtgcaggct gctttgggat
     6301 tgatccgata ctttcacatt ctcattgcct ttaaataaag cacagttaaa aacacattta
     6361 aattaagtgc gaattaattc aaatgtatat tcaattaaaa tcaaa
//
LOCUS       DAWKOG010000115         6381 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.115, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000115 DAWKOG010000000
VERSION     DAWKOG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6381
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.115"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(63..623)
                     /locus_tag="WJK66_001589"
     CDS             complement(63..623)
                     /locus_tag="WJK66_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEZ7579282.1"
                     /translation="MNMKTLLALAVSAVCSVSVAQAHEHNTIPKGASIEVKVQQLDPV
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
     gene            complement(1040..2080)
                     /gene="mutY"
                     /locus_tag="WJK66_001590"
     CDS             complement(1040..2080)
                     /gene="mutY"
                     /locus_tag="WJK66_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEZ7579283.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVEQFGG
                     TFPSERKDLETLCGVGRSTAAAICAFSFNRRETILDGNVKRVLCRVFARDGNPQDKKF
                     ENSLWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIVRNRDGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSESPSDGIWIKPTHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            2243..3326
                     /locus_tag="WJK66_001591"
                     /pseudo
     CDS             2243..3326
                     /locus_tag="WJK66_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            complement(3349..3567)
                     /locus_tag="WJK66_001592"
     CDS             complement(3349..3567)
                     /locus_tag="WJK66_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579284.1"
                     /translation="MPSEALSSLRRHFAAWRRSRHTRQHHSDILPLKCPSAFRIIKIT
                     FSSFDSSRTAHTTRLKKCRLKRIQTASA"
     gene            complement(3582..4220)
                     /locus_tag="WJK66_001593"
     CDS             complement(3582..4220)
                     /locus_tag="WJK66_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEZ7579285.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAVDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAG
                     HNSGIPLIPGVATPGEIQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(4401..6236)
                     /gene="edd"
                     /locus_tag="WJK66_001594"
     CDS             complement(4401..6236)
                     /gene="edd"
                     /locus_tag="WJK66_001594"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEZ7579286.1"
                     /translation="MNTTPIHSKLAEITGRIIERSRPTREKYLAKIHSAKQMGRLERN
                     QLGCSNLAHGYASMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAIGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRDELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLNAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKAGNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVSGELNVLINETE
                     WNAREVESIDLGANQQGCGRELFANFRSMTSSAETGAMSFGGEFA"
BASE COUNT         1419 a         1534 c         1795 g         1633 t
ORIGIN      
        1 ctaaatatct agcaaaaaag tgcggtcaaa atttaccgca cttttcgttt cgaacaatcg
       61 aattatttaa tcacgccaca tgccatacgt gggccgccac cgccaagtgg agctggatga
      121 tcggagtgat tatcaccacc cgtgtggatc ataatagagt gaccgcgaac atcatctaaa
      181 tgtttaagac gtggtgctaa aacaggattt gttgctgtgc catcatgcaa tacagttaat
      241 gcaggtaaat cacctaagtg tgcatcatct tgccatgggt aaccatgttg ttttgcacct
      301 ttaggatccc agtgaccgcc tgcgcctaaa ccagctgtca atttaccttc tttttctttt
      361 ggctcacagc ttgggttttc atggatgtgg aaaccatgta agccttcgct taatccttgt
      421 aaatcagggg taaacacaag accatagtta gattcagtaa tagtcactgt acccacatct
      481 ttgttaccgt ttactggatc aagttgttgc actttcactt caatagaagc acctttaggt
      541 atcgtattat gctcgtgtgc ttgcgcaaca ctaactgaac atactgcact aaccgctagt
      601 gctaataagg ttttcatatt cattgcaatc tcctttattg cagtaaaaac tcgcgactat
      661 tttagtcaat tttgttttaa aagccgataa atttttacga aaattttaca ttaattgtta
      721 tttgcagaaa attagaaccc gttatcacgt cttggataaa tgccaaaacc attgtgattc
      781 agagaattaa aaagaaaaat ttcggaatcc tcatcctttt ctgaaacctg attgcggtat
      841 ccatggcttt gtctttgtca tttaagtttg tgttcatgtt tttgaccttc tttgcttttt
      901 aaatcctttt taaattgccg acttcttccg ttccaaatgt cccatttgaa cattatcccg
      961 ccgcctaaaa tacgacccaa attcatcggt tgttcttgct gcgatacctg caaacaacag
     1021 ttcaatgggt ttattttgtt tattctaacc tattaccgtt taaataaatt tccaaaggct
     1081 tgggcaggcc gtaatctttc aaatgcgtcg gctttatcca aatgccgtct gaagggcttt
     1141 cagacggcat ttgtccttca aacggcgtaa tcatcagcaa ccggtgcgtc agccggtggg
     1201 tcagggcggt ttgttcgtcc atatctgcca tggtcaggga gaatttggcg gcaaagtcgg
     1261 aaagcccgtt caaactttca aaacacggca cgcaatacag cccgccccaa atgcctttgg
     1321 cggggcgttt ttccagcaaa atcgcgccgt cccggttgcg gacaatcagc cagtaaagcg
     1381 gcagggtcgg tacttcggcg gcggtttttt tgcgcggcag ctcggcggtg cggttttgct
     1441 ttttcgcttc gcagatgtcc gccatcgggc attggtggca caagggtttc gtccgtttgc
     1501 acacggtcgc gcccaaatcc atcaaacctt gtgtataggc aggcatatcg gcgttttcag
     1561 acggcagcag gctttcggca agtgtccaga gcgagttttc aaattttttg tcctgcggat
     1621 tgccgtcgcg ggcgaacacg cggcagagta cgcgtttgac gttgccgtcc aaaatggttt
     1681 cgcggcggtt gaaggagaag gcgcaaatgg cggcggcggt gcttctgcct acgccgcaga
     1741 gggtttccaa gtctttgcgc tccgacggaa acgtgccgcc gaattgttcg acgacttgtt
     1801 gcgcggcttt gtgcaggttg cgcgcgcggc tgtaatagcc caagcccgcc cacaacgaca
     1861 acacttcgtc ttgcggcgcg gcggcaagcg tctgaacggt cgggaatttt tctaagaaac
     1921 gcggatagta gtccaacacg gtggcgacct gcgtttgctg gagcatgatt tcggaaagcc
     1981 agacgcaata agggtttttg acctgccaag ggaggtggtg gcgaccgtgt tgtttttgcc
     2041 agcggatgag ccgttcggag aaggggatgg gtgtgttcat taatatcaat cggtggtttt
     2101 atttatattt aaaacagtat gttattgtat aacattgtga aaataattct tattgactta
     2161 ttttttgtag gggcatataa ctcatataag gaaactttat tgcggtattg aaattattta
     2221 tcaacaagca aggagtatca gcatgaaagc aatggtttat cacggcgcaa acgacatccg
     2281 ttttgaagaa aaaccccgtc cgcagattat cgatccgacc gatgcggtgg tgaaaatcgt
     2341 caaaaccacg atttgcggta ccgatttggg tatttggaaa ggcaaaaaaa cccgaagtcg
     2401 ccgacggccg tattctcggt catgaaggca tcggtattgt agaagaagtc ggcgaggctg
     2461 taaaaaacat caaagtcggc gataaagtca ttatttcatg cgtcagcaaa tgctgtactt
     2521 gcgacaactg caaaatccaa ctttattcgc attgccgcaa cggcggctgg attttgggct
     2581 acatgattga cggcacgcag gcagaatacg tccgcacgca ggcagaatac gtccgcacgc
     2641 aggcagaata cgtccgcacg ccttatgccg acaacagcct cgtgccgctg cccgacaacg
     2701 tcaacgaaga agtcgccctg ttgttgagcg acgctttgcc gaccgcacac gaaatcggcg
     2761 tgcaatacgg cgatgtcaaa cccggcgaca ccgtattcat cgcaggtgcc gctcctgtcg
     2821 gtatgtccgc cttgccgacc gcccaactgt acagccccgc cgccatcatt gtttgcgata
     2881 tggacgaaaa ccgtttgaaa ctggcgaaag agttgggcgc aacccatacc attaaccctg
     2941 cttccggcga tgtaagcaag caagttgccg ctatcgtcgg cgaagacggc gtggactgcg
     3001 cgatcgaagc cgtcggtatc cctgcaactt ggaatatgtg ccaagacatt gtgaaacccg
     3061 gcggccatat cgccgttgtc ggcgtacacg gtcaatccgt tgatttcaaa ctggaaaaac
     3121 tgtggattaa aaagcttgcc atcactaccg gcctggtaaa cgccaatacc accgaaatgc
     3181 tgatgaaggc aatttccagc agctccgtcg attacaccaa aatgttgacc caccgtttca
     3241 aattcagcga attggaaaaa gcctacgacg tgttcaaaca cgccgccgaa aatcaagcga
     3301 tgaaagtggt tttggaagcg gattaatcgg cgttgaatca gttgtttttt aagcggatgc
     3361 cgtctgaatg cgtttcagac ggcatttttt cagccgcgta gtatgggctg tccggctgct
     3421 gtcgaatgaa ctgaacgtta tttttattat gcggaaggcg gatgggcatt ttaggggcag
     3481 tatgtcggag tggtgctgcc gtgtatgccg actgcgccgc caagcggcaa aatgccgtct
     3541 gagactggaa agggcttcag acggcatttt tacgatgcgg ttcaggcttt gggtttcaat
     3601 gccgccgctt ctttggcgag acgggtgatg gtgtcccagt ctttgttttt cacggcttct
     3661 ttcggcgtca gccatgagcc gccgacgcac aggacgttgg gcagtgccaa gtaatcgggc
     3721 gcggtggcga ggctgatgcc gcctgtcggg cagaagcgaa catcggcgta agggccgtac
     3781 agggctttga gcatggcttt gccgccaacg acttcggcag ggaagagttt gagggtgtcg
     3841 atgccgtgtt ccaaagccag ttggatttcg cccggggtgg caacaccggg aatcaggggg
     3901 atgccgctgt tgtggccggc tttggcgagg gattcgtgca aacccgggct gatggcgaaa
     3961 accgcgcctg cgtcttcgac ggctttgagc tgttcagggt tggtaaccgt acccgcgccg
     4021 acgatggcgt tgggcacttc tttggcaatc aggcggatgg catcgaggcc gacaggggtg
     4081 cgcagggtga tttcgagggt ggggatgccg ccttcgacaa gggcgtggga caaatcgacg
     4141 gcggtgctta agtcgtcaat cgccattacc ggcacaactg cgccggcggt caaaatttcg
     4201 cggggggtca gtttggacat ttcggttctc cgggtggaat ggggtatttt attaagatgg
     4261 gacaggtcgt aggtcggatt caagaaaccg acatcttgga cggctgtgtc ggattcgaga
     4321 atccaaccta cgtctatctt aaaacacttg aataattttt cagacggcct tcggtctgaa
     4381 aaggccgtct gaaacgcgca tcaggcaaat tcgccgccga aactcatggc accggtttcc
     4441 gcgctgctgg tcatgctgcg gaagttggcg aagagttcgc ggccgcagcc ttgttggttc
     4501 gcgcccaagt cgatgctttc gacttcgcgg gcattccatt cggtttcgtt aatcaggacg
     4561 ttgagttcgc cgctaacgga gtcgaagcgg atcaggtcgc cggtacggat tttggcgatg
     4621 ttgccgccca tcagggcttc gggtgtcatg tggatggagg ccggaacttt gccagatgcg
     4681 ccggacatac gaccgtcggt cagcagtgcc actttgaagc cgcggtcttg caggatgccc
     4741 aaaggcgggg tcagtttgtg caattcgggc ataccgttgg cacgcgggcc ttggtagcgg
     4801 acgacgcaga cgaaatcgcg ttccaactcg ccgcgttcaa atgccgccaa cacttcgcgt
     4861 tggtcgttga acacgatggc aggcgcttca ataatgcggc agccttcgcg cacggcggac
     4921 actttaatca cgccgcgtcc gatgttgcct ttcatcaggc gcagaccgcc gtcgggggag
     4981 aacgggttgc cggctttgcg caggatgtcg tcgttgccgc tggtttcggg ggcttcgcgc
     5041 cattcgagtt tgccgtcgat aaggaaaggc tctttggtgt agtggcgcat accgtgtccg
     5101 acaacggtat cgacatcgtc gtgcaacagg cctgcattca gcaattcgcg gataacgaaa
     5161 ggtaggccgc cggcagcggt aaagtggttt acgtcggctt tgccgttggg gtacacacgg
     5221 atcagcaatg ggatgatgga agaaatttcg tcaaagtcgt cccagttcaa aatcacgccg
     5281 gcggcgcgtg ccatggcaac gaggtgcatg gtgtggttgg ttgagccgcc ggttgccatc
     5341 aggccgatta gggcgttgat gaaagatttt tcggtcaaca tttcacccaa tggtttaatc
     5401 gtgccgtttt tgatgccgcg cgcgaggtgt ccggcggcat agcgggtcag ggcttcacgc
     5461 aggtcggtgt aagggtggac gaaggcggcg gcaggcaggt gtacgcccat catttccatc
     5521 atcatttggt tggagtttgc cgtgccgtag aaagtgcagg tgcccgggct gtggtaagaa
     5581 cccatttcgc ttttcaaaag ttcgtctcgt ccgactttgc cttcggcgaa aagctggcgg
     5641 gtgcgggctt tttctttgtt gccgatgccg ctggacatcg ggcctgcggg gacgaagata
     5701 cccggaatat gaccgaacga aagcgcgcct atcatcaaac ccggcacgat tttgtcgcat
     5761 acgcccataa acaggctgcc gtcaaacatt tgatgcgaca gaccgatagc ggtactcatg
     5821 gcgatcacgt cgcgggaaaa cagcgacaat tccataccgg cgtagccttg cgtgatgccg
     5881 tcgcacatgg cgggtgtgcc gccggcgact tgggcggtcg cgccgttttt ctgcgcttcg
     5941 tctttaattt ggtcggggaa gtctttaaac ggctggtgtg cggaaaccat gtcgttgtag
     6001 gcggtgatga tgcctaagtt ggggacggtt tcctggagca tttcgatttt gatgcttttg
     6061 ggcatagagg catagccgtg cgccaagttg gagcagccga gctggttgcg ctctaagcgt
     6121 cccatttgtt tggcactgtg gattttcgcc agatattttt cacgcgtcgg acggctgcgt
     6181 tcaataatgc gcccggtgat ttcggcgagt ttggagtgga taggagtagt gttcacggtc
     6241 ggtctcctgt cggaacgggg ggtattgggg atttgtttgt gcgtttctga ttttttgtat
     6301 tgggtgtggg gcatttggcg tttcagacga cctttcaaac cttacaaggg gtcgtctgaa
     6361 aacctcacta tagtggatta a
//
LOCUS       DAWKOG010000116         6274 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.116, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000116 DAWKOG010000000
VERSION     DAWKOG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6274
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.116"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(94..264)
                     /locus_tag="WJK66_001595"
                     /pseudo
     CDS             complement(94..264)
                     /locus_tag="WJK66_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(293..622)
                     /locus_tag="WJK66_001596"
     CDS             complement(293..622)
                     /locus_tag="WJK66_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ7579287.1"
                     /translation="MGGLIIEDLQEGFGKEAVKGKEITVHYTGWLENGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLTIPSEMGYGAHGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            complement(700..918)
                     /locus_tag="WJK66_001597"
     CDS             complement(700..918)
                     /locus_tag="WJK66_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEZ7579288.1"
                     /translation="MLKTLTFAALHFSTAFSVTYVLTGSIGVSGAVALVEPLINTVVF
                     YFHEKAWNLYEKNKTVQQTQPFQLHRCS"
     gene            1040..1993
                     /locus_tag="WJK66_001598"
     CDS             1040..1993
                     /locus_tag="WJK66_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ7579289.1"
                     /translation="MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVRD
                     AHIVITNKVMISADIIAANPQLELIAVSATGVNNVDIGAAKAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCYYGAPIRDLNGKTLAVFGRGNIGR
                     TLAGYAQAFGMGVVFAEHKHAPDVREGYVSFEDAVQTADVLSLHCPLNAQTENMIGEN
                     ELQQMKPGAVLINCGRGGLVDENALLAALKYGQIGGAGVDVLTNEPPKNGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(2068..4125)
                     /gene="metG"
                     /locus_tag="WJK66_001599"
     CDS             complement(2068..4125)
                     /gene="metG"
                     /locus_tag="WJK66_001599"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEZ7579290.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECH
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFFIGYDNYYSTHSPENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQAE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGVDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGRLKDVADSALLAKLAAESDTIAEQYENREYARALRD
                     IMALADAVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTAAN
                     AAKFLNLEAITWANTRETLGKHAINKYKHLMQRVEQKQVDDLIEANKQSIAAAAAPAA
                     EEGKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     AFYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDTGAQPGD
                     KVG"
     gene            complement(4241..6079)
                     /gene="glmS"
                     /locus_tag="WJK66_001600"
     CDS             complement(4241..6079)
                     /gene="glmS"
                     /locus_tag="WJK66_001600"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEZ7579291.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSLASLELGPYSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKDAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPTDVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSGEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGELSPVVHTIPVQLLSYHTALARGTDVDKPRNLAKSVTVE"
BASE COUNT         1246 a         1513 c         1893 g         1622 t
ORIGIN      
        1 tttttctgcc atttgccgtg attcgggcaa tactcggtaa cggctgattt tttgaaagtg
       61 tttgaaatcg gcgcgtggtg tttttgtgca aacggcagat gacgcatcgc ggcaggttga
      121 cggcaggtgc ttggtgttga tgttgtcggc ggcggtgcgc gtaaccggct gtccgcagaa
      181 ccattttacc gaaccgtctt gacgcttggc ccacagggag agttttttgt ctttgatttc
      241 gttgtttacg ttgcttgaag ccattatgtc agacggtatt gcccgggcag ctttattcgt
      301 acactttcag cagctcgact tcaaatatca aagtggcgtg cgggggaatc acgccgcccg
      361 cgccgtgtgc gccgtagccc atttccgaag ggatggtcag cttgcgtttg ccgccttcct
      421 tcattccgcc gaagccttcg tcccagcctt tgatgacttg tccgacaccg agcgtgatgg
      481 tcagcggctg gcggcggtcg aggctggagt cgaatttggt tccgttttcc agccaacctg
      541 tgtaatgcac ggtaatctct ttgcctttaa ctgcttcttt tccgaagcct tcttgcaagt
      601 cttcaataat caggccgccc atatttgtcc tttcattgct tgttggtcaa aacggcaagg
      661 gtaacatacc gtccgtcgaa gtcaaatgcc gctcaaacgt cagctgcatc ggtgcagctg
      721 aaacggctgt gtttgttgga ctgttttatt tttttcgtaa aggttccatg ctttttcatg
      781 gaaatagaaa acgacggtgt tgattagggg ctcgaccagc gcgaccgccc ccgatacgcc
      841 tatactgccc gtcagtacat aggttacact gaaggcggtg ctgaaatgca gtgcggcaaa
      901 agtcagggtt ttaagcatca tcctctcccg aattggacat tgacggagga atgataaagg
      961 ttatcataag gttgcgcggt ttaaatttgc tatttgttgt tagtgtaaat aaatcatttt
     1021 tcaaatgggg atagaaatta tgaatcataa aaagatcgtt gttttggatg cggatacttt
     1081 gcccggccgg gtttttcatt ttgattttcc gcacgagctt gcggtttacg gtacgacagg
     1141 tgcggatgaa acggcagaac gggtgcgcga tgcacatatt gtcattacta acaaagtgat
     1201 gatttctgcc gatattattg cggctaatcc gcagttggag ctgattgccg tcagtgcgac
     1261 cggtgtgaac aatgtcgata ttggggcggc gaaggcggcg ggcgttgcgg tatgcaatgt
     1321 ccgcgcctac ggaaacgaat cggttgcgga acacgccttt atgctgatga ttgcgttgat
     1381 gcggaatttg cccgcctatc agcgtgatgt tgcggcagga ttatgggaga agtcgccgtt
     1441 tttctgctat tacggtgcgc cgattcggga tttgaacggt aaaacgctgg cggttttcgg
     1501 acgcggcaat atcggacgga cgcttgccgg atacgcgcag gcgttcggta tgggggtggt
     1561 gtttgccgaa cacaaacacg cgcccgacgt gcgcgaaggc tatgtttcct ttgaagatgc
     1621 ggtacagact gccgacgtgt tgtcgctgca ctgtccgctg aacgcccaaa ctgaaaatat
     1681 gataggcgaa aacgaattac agcagatgaa gcccggtgcg gttttgatta attgcgggcg
     1741 cggcgggctg gtggatgaaa acgcgctgct tgccgcactc aaatacgggc agatcggcgg
     1801 ggcgggtgtc gatgttttga cgaatgagcc gcccaaaaac ggcaatccct tgctgaatgc
     1861 acgattaccc aatctgattg ttacgccgca taccgcgtgg gcaagtcgtg aggctttgga
     1921 caggctgttt gatatattgt tggcgaacat tcacgccttt gtgaaaggag aggcgcaaaa
     1981 ccgcgtggtt tgagcttgtc gggatgacgg aaaacaatgc cgtctgaacg cccaaagggt
     2041 tcagacggca ttttttgaga ttcctgttta accgactttg tcgcccggct gcgcgcctgt
     2101 atccacatct aagagcttca gtttcccgtc tgccgtggcg gcactcaaaa tcatgccttc
     2161 ggatacgccg aattttgcca ttttgcgcgg ggcgaagttg gcgacggcga tgaccattct
     2221 tccgtttaac tcggcagggt tggggtaaaa cgcggcgatg ccggagaaga tgatacgttt
     2281 ttcaaaaccg aaatcgaggt cgaatttcaa aagtttggtg ctgccttcga cggcttcgca
     2341 gttcaatact ttggcgacgc gcatatcgat tttcatgaag tcgtcgaaac ttgcctgttc
     2401 ggcgactttt tcgtatttgc cctcttcggc ggcaggtgcg gctgcggcgg cgatgctttg
     2461 tttgttggct tcgattaaat cgtccacttg tttttgctcc actcgttgca ttaaatgttt
     2521 gtatttgttg atggcgtgtt tgcccaaggt ttcgcgtgta tttgcccaag tgatggcttc
     2581 caaattcagg aatttggcgg cgtttgcggc ggtttgcggc aagacggggg cgaggtaggc
     2641 ggtcaacatg gtgaaggcgt tgatgagctc gctgcacact tcgtgcaggc gttcgtcttg
     2701 accttcctgt ttcgccagtt cccaaggttt gttggcatca acgtattcgt tcacggcatc
     2761 tgccaatgcc atgatatcgc gcagggcgcg ggcgtattcg cggttttcgt attgctcggc
     2821 gatggtgtcg ctttcggcgg caagttttgc cagcaatgcg ctgtcggcaa cgtctttcag
     2881 acggccttca aagcgtttgg cgataaaacc tgaggcgcgg gcggcgatgt tgacgtattt
     2941 gccgacgagg tcgctgttta cgcgcgaaat aaagtcttgc aggttcaaat cgatgtcttc
     3001 gattttgctg ttgagttttg cggcgatgta gtagcgcatc cactcggggt ttaaaccttg
     3061 ttccagatag gatttggcgg tgataaacgt gccgcgcgat ttggacattt tttgtccgtc
     3121 tacggtcaaa aagccgtgtg cgtacacgcc ggtcggggcg cggtggccgg agaaatgcag
     3181 catggcaggc cagaacaggg cgtggaaata gaggatgtct ttgccgatga agtggtacat
     3241 ctcggtttgg ctgtcggctt tgaagtattc gtcaaaatcg acgccgatgc ggtcgcacag
     3301 gtttttaaac gacgccatgt agccgacggg cgcgtccagc cagacgtaga agtatttgcc
     3361 cggcgcgtcg gggatttcaa aaccgaaata cggcgcgtcg cgggaaatat cccagtcgga
     3421 cagggtggtt tcttcacctt cgcccagcca ttctttcatt ttgttgaggg cttcggcttg
     3481 cagatggggc ttgccgtcgt gcgggttgtt gccggaagtc catgctttga ggaagtcggc
     3541 gcattctccc agtttgaaga agaagtgttc ggattcgcgc aattcgggtt tcgcgccgga
     3601 aacggcggaa tacgggttaa tcagttcggt cggggaatag gtcgtgccgc agacttcgca
     3661 gttgtcgccg tattggtctt gggcgtggca tttcgggcat tcgcctttaa cgaagcggtc
     3721 gggcaggaac atttgttttt cggggtcgaa aagctgctcg atgacgcggc tttcgatttt
     3781 gccgttggct ttcagtgcgc ggtaaatgtc ttgggaaaac tgtttgtttt caggggaatg
     3841 ggtgctgtaa taattgtcgt agccgatgaa aaaaccggta aagtcggcga ggtgttcttc
     3901 gcgcactttg gcaatcatgt cttcgggcgc aatgccttgt ttttgcgcgg caagcatcac
     3961 gggcgtgccg tgggtgtcgt cggcgcagca gtagtggcac tcgtggccgc gcagtttttg
     4021 aaagcgcacc caaacgtcgg tttggatgtg ttcgaccatg tggccgaggt ggatgctgcc
     4081 gttggcatag ggcagggcgg aggtaacgag gattttgcgt gtcatattgt gctttgcaaa
     4141 caatgggtaa aggcggatta taccgcaaat caaacgggga aatgccgtct gaagcctgaa
     4201 aaatcgggct tcagacggca tttttgccaa ccggcgggat ttattcgacg gttacggatt
     4261 tcgccagatt gcgcggcttg tctacatccg tgccgcgcgc gagggcggtg tggtaggaaa
     4321 ggagctgcac ggggatggta tgtacgacgg gggacagttc gccgacatgg cgcggcgcac
     4381 ggataacgtg cacgccttcg gttgcattaa aattgctgtc gaggtcggca aagacgaaaa
     4441 gttcgccgcc gcgtgcgccg acttcctgca tattggcttt gactttgtcc aacaggctgt
     4501 cgttgggcgc gatgacgacg acgggcatgt tttcgtccac caaggcaagc ggcccgtgct
     4561 tcagttcgcc ggcaggatag gcttcggcgt ggatgtaggt gatttccttc agctttaacg
     4621 cgccttcgag ggcaatcggg taatggatgc cgcgccctaa aaacagcgcg ctggttttct
     4681 tggcaaactg ttgcgcccag gcggcaattt gaggttcgag gtttaaagcg tgctgcacgc
     4741 tgccgggaag ctggcggagt tcttcggtgt aacgtgcttc atcttcgccg gataccaaac
     4801 cgcgcacttt cgccagcgtt accgccaaac cgaacagcgc gaccagctgc gtggtaaacg
     4861 ccttggtcga ggcgacgccg atttccgcac cggcacgggt ataaagcacg aggctgcttt
     4921 cgcgcggcag ggcggattcc atcacgttgc agatggagag gctgtggcgg tgtcccaaag
     4981 atttggcgta tttcaacgcc tccatcgtgt ccagcgtttc gccggattgg gaaatggtaa
     5041 tgaccagttg gtccggatcg gcaatcacgc tgcggtatcg gtattcgctg gcgatttcga
     5101 cgtcggtcgg aattttggcg atggattcga gccagtattt tgcggtcagc gcggcgtaat
     5161 aggacgtgcc gcaggcaagg attttgacgc tgcggatgct ttcaaacacg cttttggcgt
     5221 ctttgccgaa gttttcggga atgaagccgc cgtcgaggaa aacctccgcc gtgtctgcaa
     5281 tcgcgcgcgg ctgctcatgg atttcctttt gcataaagtg gctgtacggt cccagttcca
     5341 aagaggcgag cgagagttcg gataccttga ctttgcgttc ggcaggcagg ccgtttttat
     5401 cggtcagcct tttgatgccg tctgaagcca gcagcgcgat gtcgccgtct tcgaggtacg
     5461 ccacgcggcg cgtaaaggcg atgacggcgg atacgtccga agcgataaag gtttcatcgt
     5521 cgcccaaagc gaccaaaagc gggcagccca tacgcgccac aactaattca tcaggcttgt
     5581 cttgggcaat aaccgcgatg gcgtatgcgc cgtggaaacg tttgaccgct tcttgtaccg
     5641 cttcaaacag cctgccgccg ttttgcgcgt attcgtgatt gatgctgtgt gcgatgactt
     5701 cggtatccgt ttgcgattca aaacggtatc ccaaaccttc caaacgtttg cgttcgcttt
     5761 caaagttttc gatgatgccg ttgtgtacga ccgcaatcat accgccgctg atgtgcgggt
     5821 gggcgttcgg ctcagtaacg ccgccgtgtg tcgcccaacg cgtatgtccg atgccgatgc
     5881 cgccgctgat gcctttttcg cgtgccgcgt cctccataag ctgcacgcgt ccgacgcggc
     5941 gcacgcgttt gattttgccg tcggtgttga cggcaatgcc tgatgagtca tacccccggt
     6001 attcgaggcg tttgaggccg tcggtcagaa aatcgacgac gttgtgatgg gcgcggatgg
     6061 cgccgacgat accgcacata actgttcctt agtatcaggt tgaaaaaaaa caggcgcgga
     6121 cggctcccgt gccgcacctt ccgcttcgga ttataaaccg cctagtgtcg gaaaacagca
     6181 aaatgccgtc tgaaggcttg ggcttgctca aaaaaggagg gatttccccg cctgaccggg
     6241 atgggcgttc agacggcatt acctgccgcc ggtt
//
LOCUS       DAWKOG010000117         6226 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.117, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000117 DAWKOG010000000
VERSION     DAWKOG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6226
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.117"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(365..2452)
                     /locus_tag="WJK66_001601"
     CDS             complement(365..2452)
                     /locus_tag="WJK66_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEZ7579292.1"
                     /translation="MDYLSRLSFGFNKKLPVILQTEVAECGLACLTSILSYYGFHTDL
                     RTLRQKYTLSLKGANLADIMRFGNEMNLTPRALRLELDELSNLQLPCILHWNLNHFVV
                     LCSISKDSIVIMDPAVGMRKIKMDEVSQKFTGIALELFPNTHFEEKKETKKIKILSLL
                     RGISGLKRSLIQMLILAISLEVFALVSPFFMQWVIDHVIVTADKNLLLTLTLGFGLLA
                     ILQQLISLLQAWVGMHLSTTLNLQWKANIFKRLLDLPNDYFSKRHLGDVISRFGSIDH
                     IQETLTSTFFVLVLNSLMAVFTFVLMTIYSTQLSLIVLLTLVLYILIRWLAYYPLRNA
                     TEENIVHEAKQNSYFMETIRGIQSVKLFDKHYQRHGTWMSLFVNTVNTKLTTDKLSAL
                     FEFSNKLLFSMENIIIIYLGASAILDGSFTVGVLMAFLAYKGQFESRTASLVDQYIQI
                     KMLGLHAERLADITLNETETEIIKYNHIPKLDNEQLVLKVENVSFRYADNEPYLFENI
                     NLEFKDNEAVVLTGQSGRGKSTLLNILTGSLKPETGTVSINGHDIYQVSPSFIRGLSG
                     IVRQDDVLFAGSIGENISFFDESPNMELIEQCAKMAQIHDDILKMPMGYETLIGDMGN
                     ILSGGQKQRVILARALYKRPKILFLDEASSHLDVENEQKINHNLKSLGIMKIMVAHRQ
                     ETIQSADKILNLG"
     gene            complement(2455..3720)
                     /locus_tag="WJK66_001602"
     CDS             complement(2455..3720)
                     /locus_tag="WJK66_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEZ7579293.1"
                     /translation="MSHLFRKEVFVAQQNKWTGQVILTRPFSFLFLTFCAFLIALCII
                     IFLIFGSYTNKTTVEGQLLQTMGVVRVYSPDIGTITHKFVEDGNFVKAGEPLFKLSTS
                     RFGEKGNVQAKLAAEANLKKTLALQELERLKRIHQNEQKNVHNNIHRLNNQLENIKQQ
                     ITGQNRQIRLAEKTLNKNKFLASQGAVSQQDKMTAESHLLEQRSRLESLKLEQNNAIR
                     ELDEQKITLSSLPERHKTELSQLNRAITEMNQEILDFDLKSEQTIRASKSGYISTINV
                     DIGQQVEPSKLLLSIVPEQTELVANLYIPSKAVGFIKPKDKVVLRYQAYPYQKFGHAT
                     GEIISVARTALGKQELSGLGIIFTNPTLLNEPAYLVKVKLEKQTIKAYGENKPLQIGM
                     ILEADILHERKKLYEWVLDPLYSISGKIN"
     gene            complement(3687..3901)
                     /locus_tag="WJK66_001603"
                     /pseudo
     CDS             complement(3687..3901)
                     /locus_tag="WJK66_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235355.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4943..5179)
                     /locus_tag="WJK66_001604"
     CDS             complement(4943..5179)
                     /locus_tag="WJK66_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HEZ7579294.1"
                     /translation="MKELHTSELVEVSGGKFHISAQGGGNLGKKDMVAVGKIGASYSP
                     NNSGVEFSVSKQFGYVQGLGVQFSKPTFGFSKKW"
BASE COUNT         1975 a         1106 c         1084 g         2061 t
ORIGIN      
        1 tgccaatcaa tcacctggtc caacttcaat agtgggaaac ggtcgatgtg tttggcaatc
       61 atggcttggg cggtttgttg gaagaaggtg ctcatgagaa atcccctaaa tgtcatggtg
      121 ggaatttagg gaattttggg gaattttgca aaggtctcag accgtgaaat tagcccaaaa
      181 atgggaacaa gagcagaaaa aacaacaaat tcaacagaaa aaggaaactg aaaaatcgcc
      241 aaaacacaaa gccagtcgtg atgattggga aatggaacgt taaaccgcta ttttcatgta
      301 ttctattaaa ttatttgaaa aaagacttca ctttttgtta aagaaaaatg aagtcttgtt
      361 ctgttcaacc taaattcaga attttatctg ccgattgaat tgtttcttgg cggtgtgcaa
      421 ccattatttt cataatacca agacttttta ggttatggtt aattttttgt tcattttcta
      481 catctaaatg gctacttgct tcgtctaaaa atagaatttt gggtcgttta tacaatgcac
      541 gagccaagat aactctctgc ttttgtccac ctgataagat atttcccata tcgccaatca
      601 aggtctcata gcccattggc attttaagta tatcgtcatg tatttgtgcc atttttgcac
      661 attgttcaat gagctccata tttgggcttt catcaaaaaa tgaaatattt tccccaatag
      721 aacctgcaaa aaggacatca tcttggcgaa caatcccgct caatccccta ataaaggatg
      781 gagaaacttg atatatatca tgcccattaa tactaactgt accagtttca ggttttaggc
      841 tacctgttaa aatgtttaac aaagtggact tcccccgacc agattgtcct gttaaaacaa
      901 ctgcttcatt atctttaaat tccaaattaa tgttttcaaa aagatatggc tcattatcag
      961 catatctgaa tgagacgttt tcaactttaa gaaccagttg ttcattatct aatttaggta
     1021 tatgattata cttaataatt tcagtttctg tttcatttaa agtaatgtca gccaaacgtt
     1081 cagcatgaag ccctaacatt ttgatttgga tgtattggtc aacgagagaa gctgttctgc
     1141 tttcaaattg ccctttataa gccaaaaaag ccatcagaac accgactgta aatgaaccat
     1201 ctaaaattgc gcttgcacca agataaatta tgataatatt ttccatgcta aacaacagtt
     1261 tatttgaaaa ttcaaataaa gcagagagtt tatctgttgt cagcttggta ttgactgtat
     1321 tcacaaatag gctcatccaa gtgccatgtc tttgataatg tttatcaaat aatttaactg
     1381 attggatacc acgaatggtt tccatgaaat atgagttttg tttggcttca tgaacaatat
     1441 tttcttctgt tgcatttctt aatgggtaat atgcaagcca acgaattagt atgtacaaaa
     1501 caagtgttaa aagaacaatc agcgataatt gagtgctgta aattgtcatt aacacgaaag
     1561 taaaaacagc cattaagcta tttaaaacta aaacaaaaaa agtagaagtt agtgtttctt
     1621 ggatatgatc tattgaacca aatcttgaaa tcacatctcc taaatgtcgt ttactgaaat
     1681 agtcattagg taagtcaagt aaccttttaa atatattggc tttccattgt aaattaagag
     1741 ttgtagatag gtgcatacct acccatgctt gtaacaggct aattaactgt tgcaggatag
     1801 ccagtaaacc aaatcccaaa gtaagggtca gtaataaatt tttatcagca gttacaatga
     1861 catggtctat tacccattgc ataaagaatg gactaaccaa tgcaaagact tccaaagaaa
     1921 tagctaatat aagcatttga attaaagagc gttttaagcc tgaaatcccc cttaatagag
     1981 ataatatttt gattttcttt gtttcttttt tctcttcaaa atgggtattg gggaataatt
     2041 ctagggcaat ccctgtgaat ttttgtgaaa cttcgtccat tttgattttt cgcataccga
     2101 cagcagggtc cataatgacg atactgtctt tggaaatgga acaaagtaca acaaaatggt
     2161 ttaagttcca atggagaatg cagggtagtt gtaaatttga cagctcatct aactctaaac
     2221 gcaaagctcg tggcgttaaa ttcatttcat tgccaaatct catgatgtct gcaagatttg
     2281 cgccctttaa tgacagggtg tatttttggc gtaacgttct taaatcagtg tgaaagccat
     2341 aataggacaa gatggatgtc aggcatgcta aaccacattc agcaacttct gtttgcagaa
     2401 tgacaggtag ctttttgtta aatccaaagg acagtcttga taaataatcc atttttaatt
     2461 gatttttcct gaaatgctgt aaagtgggtc aagtacccat tcgtacaatt tttttcgttc
     2521 atggagaata tctgcttcta aaatcatgcc aatttgaagc ggcttgtttt ctccgtatgc
     2581 tttaatcgtt tgtttttcca atttaacttt cacaagatag gcaggttcat ttaataaggt
     2641 tgggttagtg aaaatgatac ctaaacctga tagctcttgt ttaccgagag cagttctggc
     2701 aactgaaata atttctcctg tggcatgtcc aaatttttgg taagggtacg cttggtaacg
     2761 taaaacaact ttatctttcg gtttaataaa accaacagct ttactgggta tgtaaagatt
     2821 ggcgaccaat tcagtttgtt cagggacaat gcttaacagc aatttagacg gttcaacttg
     2881 ttgccctata tcaacattaa ttgttgatat ataacctgat ttactagctc gtatggtttg
     2941 ttcggatttc aaatcaaaat ccaaaatttc ttggttcatt tccgtaatcg cacggttgag
     3001 ttggctcaat tcggttttat ggcgttcagg caggctgctt aatgtaattt tctgttcatc
     3061 aagttccctg attgcattat tttgttcaag ttttaggctc tccaaacgtg agcgttgttc
     3121 caataaatgg ctttcggcgg tcatcttatc ttgttgggat actgcgcctt gactggctaa
     3181 aaacttgttc ttgttaaggg ttttttccgc taaacgaatt tgacgatttt gccctgtaat
     3241 ttgctgttta atattctcta attgattgtt taaacgatga atgttgttat gaacattttt
     3301 ttgctcattt tgatgaatgc gctttaaacg ttccaattct tgtaatgcca aagttttttt
     3361 aaggttggct tctgctgcca atttggcttg tacgtttcct ttttcgccaa aacgcgatgt
     3421 ggaaagtttg aacaatggtt cgccagcttt gacaaagtta ccatcttcaa caaatttatg
     3481 cgtaatcgtg ccgatatcgg gagagtaaac acgaaccacc cccatagttt gaagtaattg
     3541 accttcaacg gttgttttat tggtatagct accaaaaatc aaaaagataa tgatacacag
     3601 agcaatgaga aaagcgcaaa aagtcagaaa taaaaaagag aatggacggg tcaagataac
     3661 ctgacctgtc cacttatttt gttgggctac aaagacttct tttcggaata agtgggacat
     3721 ttattattat ttcttaaaaa aatggataga aaaacaccaa taattaaata cttatgccta
     3781 ataacataaa tttgctattg attgatgttt ctattttaat tacattaaat ttaaccaata
     3841 atccagaaat ggttgcagct aaaatgcaaa agacagtggc gtaaatggtt tcagtattca
     3901 taatttttct ctttcaaaat tgttcaatct aaatagaaaa aggtaacttc aatacatatt
     3961 gctgtaactg aaattacctt ttccttaatg atgtaattat cttatggctt catcatgcca
     4021 cgaccgatat tgtatccgcc agcagcagag aaaataccag ctccaattgc gccaacccag
     4081 cctactggtc ctgtccactt gttccgttga gctacaaaga cttctggtct gaaaaatgag
     4141 tgattgttag acatgtgtac ttatttatgt ttctttgatt gggataaatt tgtaaaaaca
     4201 aaaaaaaagg caagttgtta ttatagaaga acttaaaatg cttaacatat tcattgaggt
     4261 ttgtaccaca taatgagata cgagataatt tgttgggagt aaaatcaaaa aaaagattaa
     4321 atatttaaaa taatcactat attttttcat tatttgttcc tttttgtatt taagttctta
     4381 tgaaaaacta gggtattttg aatagaaaat atcaaaatac cctagtttta ttaaggaata
     4441 ttaccaccaa caagtgcttc catattgtac agcaccagtg attgcaccac caatagctcc
     4501 gaatttcgca cctggaatag caccaatacc acctgcaaat gaaccaacaa ttgccccacc
     4561 agctgctcca cctaatgcac taccaatggt attttttgag aaatcacgcc agttacaagc
     4621 agcaccagaa acttgttaaa tttcattcaa agtcaaaact tgcataatat tcacctttgt
     4681 taagtgtggt tgagaaaaaa gtaaattttc atcgttactc attatttgca atcatgcata
     4741 tactgtaacc aagcactata ttcagttgga tcatagtttt tcctactggg ttgtctgata
     4801 tgactattgg cactattggc acaattggga ttagcaactt tattgtgagt acctaatgga
     4861 ttgatatttc ctgtaaagtg gctattgcat gcgcttaatg agcataaatt tattaatgtc
     4921 agaaaggata aaacaaaaaa tcttaccatt ttttactaaa accaaaagta ggtttcgaaa
     4981 actgtacacc aagaccttgt acatatccaa attgcttgct aacagaaaac tctactccac
     5041 tattgttagg ggaataggaa gcaccaattt taccaacagc aaccatatct tttttaccta
     5101 ggttgccgcc accctgtgca gagatatgga atttgccacc tgacacttca actaattcag
     5161 aggtgtgtaa ctctttcatg attttctcct gtaaagaagt atggttggaa atgtaaatca
     5221 gaattaacct ttcttaagtt taatttaata cttttttttg caagtatttt attttaaatt
     5281 aaatcaatat tttaaaaata tttacttgta tactgaaaaa gaaactccta atggtggagt
     5341 ggggcttttg tttaagtaag cattgaaaaa atttaaattt cccattatga ttaacttaat
     5401 gcaagagctt tttcaggacg tacaaaccag cgaacgggcg ttctgatgtg ggcaggttgg
     5461 ttgtcgctaa ccaatttcag tagctcaatc gtcagattgg tatccgaccc tatcaagcta
     5521 aggagaggct gcggctacta cacaaagcac ctgaaattat tgggtgcttt tgtcttttta
     5581 tggcattgta ccaatttgtg taccacgagg gcttgttcgc ccaaacgcgg taacaactga
     5641 atacacactt tccccctcaa cttctttgcg ttctaaaacg actgtcccaa tccgagaata
     5701 gacaatcata ggacgatgat tgccccttac atcactaaat tcactaaaaa tttttgcgcc
     5761 tgattgaaaa atcatttcca cataaaaggg ttacacctgt ttcatcgtgg cttaatccaa
     5821 accgcgttaa atctgctttg tgttctgcca aaatgtgaat cacaccaaag ccgtgattaa
     5881 cctttttgtg ttcgccgtgt tttaaaacaa tttgacctgc cgagaagtct gctctattgt
     5941 tcggacaata gggggaatat accaattact ttaactacat cattttttga catttttctt
     6001 gcctttcaat ttaaaatcca aaatcatact gccataattt agcaatccaa aaaaatttag
     6061 gcagcagtca tggtttcgta ttttagaaaa cgaaacttgg tttggtgttt tgaagcattt
     6121 cagtacacaa acatggaatt ttaaattgtc ttgtgttgcc ttgatttagg atgttccagt
     6181 tttttaataa atagtttggg gctgtcctag ataactagga caaact
//
LOCUS       DAWKOG010000118         6171 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.118, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000118 DAWKOG010000000
VERSION     DAWKOG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6171
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.118"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            241..687
                     /gene="yciA"
                     /locus_tag="WJK66_001605"
     CDS             241..687
                     /gene="yciA"
                     /locus_tag="WJK66_001605"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HEZ7579295.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIAQGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVEVWVKTL
                     MNDCVTEDRHLVTEAVFTYVAIDAEGNPRPIPKEGNPILAGLLPTP"
     gene            complement(738..1559)
                     /locus_tag="WJK66_001606"
     CDS             complement(738..1559)
                     /locus_tag="WJK66_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ7579296.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPNMVARGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDEKAAGVYEGVEFIRPEDIAETALW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            1678..2136
                     /locus_tag="WJK66_001607"
     CDS             1678..2136
                     /locus_tag="WJK66_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ7579297.1"
                     /translation="MKTQISLAAAAITLLLSACGGSGIPSQPKGEISENRTAAFKSMM
                     PDFSRMGKMVKGEEPYDVEKFKQAAAAFADSSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(2300..3415)
                     /gene="lst"
                     /locus_tag="WJK66_001608"
     CDS             complement(2300..3415)
                     /gene="lst"
                     /locus_tag="WJK66_001608"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HEZ7579298.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFHLPYGLNKSFNFIPTMAELKVKSMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTGNLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKD"
     gene            3516..3620
                     /locus_tag="WJK66_001609"
                     /pseudo
     CDS             3516..3620
                     /locus_tag="WJK66_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            3848..6073
                     /locus_tag="WJK66_001610"
     CDS             3848..6073
                     /locus_tag="WJK66_001610"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEZ7579299.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWTKDSKTHVATMQSGDFFHNEQSVTVPDATSVSIVFTDKQGNKKELRAPVALK
                     AGEITDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGGKLAAAGVNVNNGFGNLLANLDKLDADTRAAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKNGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAEGKVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRILAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDEDAELK
                     AAFAPLAAALTADEAKIVEELSAVQGKAVDIGGYYAANPEKAAQAMRPSVTFNQALNA
                     L"
BASE COUNT         1647 a         1813 c         1374 g         1337 t
ORIGIN      
        1 agacctgtcc gcacagaaac ttatcggata aaaacagttg cccaaacccc gcgttctaga
       61 ttcccacttt cgtgggaatg gcggttcagt tgctacggcc ccgccctaca atacgccttg
      121 cgaatctgtc ccacgcccgc ccttgtatgc ctcaaatggg ttaaactacg cacttcccga
      181 cttcccgttt cgggcagggg tgcaggttcg atttatcttt caataaaaca aggaaacttt
      241 atgcagcacg aagaaggcaa ccgccaacgc cctcaaggcg aactgctcct gcgtaccgtc
      301 gctatgccgc gcgataccaa tcccaaccaa gacattttcg gcggctggat tatgtcgcag
      361 atggatttgg gcggcggcat attggcagcg gaaatcgcac aaggacgcat cgttaccgtc
      421 gccgttcagg aaatgaactt catccgcccg gtcaaggtcg gcaacgtcgt ctgctgctac
      481 gggcattgcg tccgcgtggg caatacttcc ctccagctta aagtcgaagt ctgggtgaaa
      541 actttgatga acgattgcgt taccgaagac cgccacctcg taaccgaagc cgtgttcact
      601 tatgtcgcca tcgatgcgga aggcaatccg cgcccgattc cgaaagaagg caaccccata
      661 ttggcgggct tattgcctac tccgtaaaaa tgcccgtaaa aatgccgtct gaaactgtct
      721 tcagacggca ttccgcatca tttgaaccaa gatctgatgc gggaaaacag cgacatactc
      781 tgtttttcaa agtcttcccg cacttccggc acggcttttt ttatcacctt cattcctgca
      841 aaagtctgcg ccacgggcat aatttcaatc gtgttcacat tcatatgcgc cggccggcgg
      901 tacagccaca atgcggtttc cgcaatatct tcggggcgga taaattccac accctcatac
      961 acgcctgctg ccttctcgtc atcgcctttg aagcgcacgt tggaaaactc cgtattgccg
     1021 cacaaacccg gctcgatatt ggtaacgcgg atgttcttat ccgccaactc cgcgcgcaaa
     1081 ttcaggctga actggcgcac aaacgccttg gtcgccccgt aaacgttgct gccggcataa
     1141 gcataattgc ctgcaatcga acccaaattc atcacataac cgccgccccg agccaccata
     1201 ttcggcaaaa tcttgcgcgt tagaaacgtc aaacccaaaa cattggtttg aatcattgtt
     1261 tcccaatctt caaaatccgc cttgtccgcc gtgtccagac ccaaagccag cccggcattg
     1321 ttgatgaggc agtcgatgtc ggaaaattca tcagggatgc cgtttaaggc gttttccacc
     1381 gactcgcggc gcgacacgtc catttccaaa gggtaaaaca aagcacccaa ttcatccgcc
     1441 aaggcctgaa gcctgtccgc acgacgcgcc gcaccgataa cgcggtatcc cgccccgaca
     1501 aacgcgcggc acatcgcttc gccgaatcct gccgaagcac cggtaattaa aacagccatt
     1561 gtttttcctt tctttctcgt ttcccaaacc tgtttcaggt atcataacgc tcttcagacg
     1621 gcatcgcgcc ggctgaaaat gacaacaacc ataccaccac gacaggacac attcaatatg
     1681 aaaacccaaa tcagccttgc tgctgccgcc atcacactgc tcctttccgc ctgcggaggc
     1741 agcggaatac cttcccaacc caaaggcgaa atttccgaaa accgcaccgc cgcgttcaaa
     1801 tctatgatgc ccgacttctc gcgtatgggc aaaatggtca aaggcgaaga gccttacgat
     1861 gtcgaaaaat tcaaacaggc ggcagcggcg tttgccgaca gcagcaaaaa accgtttacg
     1921 ctttttgagt ccgacccgca aggcaacggg cgcgcactgc ccgccgtttg gtcggatggt
     1981 gcaaaatttg aagccgaaaa aacaaaattc gccgccgccg tcgaaaaact caacgccgcc
     2041 gcccaaaccg gcaaactgga cgaaatcaaa gccgcctacg gcgaaaccgg cgcaagctgc
     2101 aaatcctgcc acgacagttt ccgcgcgccc gaataacatc caaaacaaaa caatgccgtc
     2161 tgaagccttc agacggcatt ttctttgcca tttcaccaag cgcaaagcaa tcaaaaatta
     2221 tcgttaattc aaaaaatacg ccatatcctg atttttgcct tacttactcc gccattttct
     2281 attttgattt gctatgcgat taatctttat cgtcaaatgt caaaatcggg atgccggatt
     2341 gggtaaacag ggcatacacc ggcttgagcc aataatcttc cggaagggaa gccggtttca
     2401 atgcgtaaac gtgcacattg ggaaaatcct tcatcgtcaa cgccgcgccg ctgaaaaagg
     2461 tataaatttc atacctcgta tgcgggtttt tcttaatctc gcgcaaaata tagtcttcga
     2521 tgacataggg cgaatttaat gtggttacgc cggaaagccc gtaggtttgg cgggggtgcg
     2581 gtgcgacata ttgtatgttg aagttttttg ccgccttttc cgaaatttcc ttcatctcct
     2641 tgtcgggcga acccaaaagt atccgcaccg tgccgcccgt ttcgtccccc gccttcagtt
     2701 cggacgcatc gaacagcggc aggtaagtca tcttgcggcg gccgtcgtcc ataatgtttt
     2761 tcaaaccctt gaatatcgtg taatgctcgt cggaagcatt acgggttttg gcgatgctcc
     2821 aatctccgat catcatccgg gcaaaattcc gcttgatcgt cccgtttaca gaaaactcat
     2881 cgcccaaata gctgctgctt tgaattaaat tgcctgtccc gtcgtcaaag gttttgattt
     2941 ccgcatccgg gtaagtgctc aaaaaggcgg caatgctgac tttttccaaa cttgccaaat
     3001 aaatccgctt gactttcggc agcagcatcg actttacctt cagctccgcc atcgtcggaa
     3061 tgaaattaaa cgatttgttc aaaccgtagg gcaggtggaa aaaatacgcc cgctccgcct
     3121 tatcctttat ctgattgaaa taataatcgt atttttcatt cctgttttca gacatcagca
     3181 ccacataaaa ccgctccccc ggatgctgcg ccataatcct ttccgccacc ttcatctgca
     3241 atatggtata gcagaaaatc agattgaccg gttccccctc ttcattgaag agtttgtcct
     3301 tcagcaggga aaccgcattc ctttccccct gatttacccg gtcaaatgta taaaatatcc
     3361 cgaaacaaaa aacaatcaaa cacaacacgg tcaaacaagc ctttttcaag cccatatccc
     3421 taaaactcca ttccgataaa ttgaacatac gccccgagtg tcccgggacg gaaaaatgcg
     3481 gaattttagc agatattccg acagttaccg aatattatag tggtttaaat ttaaatcagg
     3541 acaaggcgac gaagccgcag acagtacaag cagtacgaca aggtgaggca acgctgtact
     3601 ggtttaaatt taaaccacta taaaatcaat ttcaccgaaa atatgccgcc gcacatcagg
     3661 cacagcacct ccgaccgcca ccaaacgcgg gaaaaccatt tcccccttcc tttcacgctg
     3721 ccgcgttcaa acacgccaag atcgaacaaa gcgcgaacaa agccgccaaa atatgccata
     3781 atagccgcgc acattccccg cgcaatgtgt tccaaaagaa aaaaccaacc tacaagagaa
     3841 taatcctatg actcaaaaat ccaccattgt ttatacccat accgacgaag cccccgcgct
     3901 ggcgacccaa tcgctgctgc cgattgtgca ggcttttgcc cgccacgccg atattgatgt
     3961 caaaactgcc gacatttccc tgtccggccg tattttggcg gcattccccg aatatctgac
     4021 cgaagcgcag cgcgtacccg acgcgcttgc cgaattgggc gaactggtga aacagcccga
     4081 tgcaaacgta atcaaactgc cgaacatcag cgcgtccgta cctcaactga ccgccgcgat
     4141 taaagaattg cagtctaaag gctttgccgt tcccgactat cctgccgacc cgcaaaccga
     4201 tgaagaaaaa gccgtacgcg aacgctacga ccgcatcaaa ggcagcgcgg tcaaccctgt
     4261 cctgcgtgaa ggcaactccg accgccgcgc ccccaaagcg gtgaaaaact ttgccaaaaa
     4321 aaatccgcac agcatgggcg cgtggacaaa agactccaaa acccacgttg ccaccatgca
     4381 aagcggcgac tttttccata acgaacaatc cgttaccgta cccgatgcaa cttccgtatc
     4441 catcgtgttt accgacaaac aaggcaacaa aaaagagctg cgcgcgcccg tcgccctgaa
     4501 agcgggcgaa atcaccgacg cgaccgtgat gagcaaaaaa gccctgctcg ccttccttgc
     4561 cgaacaagtg aaagacgcaa aagctaaagg cgtattgttc tcgctgcata tgaaagccac
     4621 gatgatgaaa gtgtccgacc cgattatctt cggacacgcc gtcaaagtgt tcttcgcgcc
     4681 tgtatttgaa aaattcggcg gcaaactggc tgccgcaggc gtcaacgtca acaacggctt
     4741 cggcaacctg cttgccaatc tagacaaact ggatgcggac acccgcgctg ccgttgaagc
     4801 tgaaatcgcc gccgtttacg ctgccaaccc tgatttggca atggttgatt ccgacaaagg
     4861 cattaccaac ctgcacgttc ccagcgatgt catcgtcgat gcctctatgc ctgcgatgat
     4921 tcgcaattcc ggccgtatgt gggacaaaaa cggcaaagcg caagacacca aagccgtgat
     4981 tcccgacagc agctatgccg gcgtttacca agcaaccatc gacttctgcc gcgaacacgg
     5041 cgcgttcgat ccgacaacaa tgggtactgt tcccaatgtc ggtttgatgg cgcaagcagc
     5101 ggaagaatac ggttcacaca acaaaacctt tgaaatcgaa gccgacggtc aggttcaagt
     5161 cattgatgcg gaaggaaaag tcctgatgca gcacgacgtt gaagcaggcg acatctggcg
     5221 tatgtgccaa accaaagacg ctccggtcaa agactgggtg caactcgccg tcaaccgcgc
     5281 ccgcctgagc aacacgcctg ccgtgttctg gctcgatgaa aaccgtccgc acgacaagag
     5341 cctgctcgcc aaggttaaag cctaccttgc cgaactggat accaatggcc tcgacatccg
     5401 catcctcgct cctgaagaag ccgccaagtt cagcttgggt cgtctgaaaa acggcgaaga
     5461 caccatctcc gtaaccggta atgtcttgcg cgactacctg accgacttgt tcccaatttt
     5521 ggaactcggc acaagcgcga aaatgctgtc tatcgttcca ttgatgaacg gcggcggtat
     5581 gtttgaaacc ggcgcgggcg gttctgcacc gaaacatgtt caacaattcc tcgaagaaaa
     5641 ccatttgcgc tgggactcgc tgggcgaatt cctcgcactc gccgtatcgt ttgaacattt
     5701 ggcgcaaaaa accggcaatg ccaaagccca agtcctcgcc gacactttgg atgcagccac
     5761 cgaaaaactg ctgttgaacg acaaatcgcc taaacgtaaa gccggcgaac tcgacaaccg
     5821 gggcagccat ttctacctta ccctctactg ggcgcaagaa ttggcggcgc aagacgaaga
     5881 tgccgaactg aaagccgcat ttgcgccatt ggcagccgct ttgactgccg acgaagccaa
     5941 aatcgttgaa gaactttctg ccgtacaagg caaagcggtc gacatcggcg gctactacgc
     6001 cgccaatcct gaaaaagcgg cacaagcgat gcgcccgagc gtaaccttta atcaggcact
     6061 aaacgcctta taagcacaaa ggtaaaaatg ccgtctgaac attcgtcgtt cagacggcat
     6121 tagttatccc tatctgcctg attatgatta aacgccgcac ccattggata t
//
LOCUS       DAWKOG010000119         6138 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.119, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000119 DAWKOG010000000
VERSION     DAWKOG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6138
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.119"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(117..2123)
                     /locus_tag="WJK66_001611"
     CDS             complement(117..2123)
                     /locus_tag="WJK66_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEZ7579300.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TTPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGIIIALMALLKIRIGTLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRQPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLAGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
     gene            2410..4437
                     /locus_tag="WJK66_001612"
     CDS             2410..4437
                     /locus_tag="WJK66_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEZ7579301.1"
                     /translation="MSLSEFIERRTSFNLMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEIIFTEFSWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLSLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            complement(4529..6082)
                     /locus_tag="WJK66_001613"
     CDS             complement(4529..6082)
                     /locus_tag="WJK66_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEZ7579302.1"
                     /translation="MNRTYANFYEMLTAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTGQSRPDGEMAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQALLKRATVFLGVPAIYTAMSKTKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
BASE COUNT         1264 a         1437 c         1869 g         1568 t
ORIGIN      
        1 ttaaaatgcc gtctgaacca cgtttcggtt cggacggcat ttttgcatcc ttttatgggg
       61 atttcccgca tcagggcggt attcggggcg aatcccgctt cagacggcat tgccggtcaa
      121 ggcttgtccg aaggaggatg tccctgttct ttggcgggcg ggaggaaaag tatgcaggga
      181 ttgcttttaa tctgattcca aaaactctta atcagattgc ggatgctgcc gattttcgtg
      241 ccgcgcgagc gcagggcgac gaagagggcg aggctgaagc tgacgcagag gttgaccagg
      301 ccgatggcga ggacgctgaa aatgccgagt acgaacgtgc ccaaaccgac gttgccgctg
      361 acggcggcat agccgaggtt ggcggaggaa aaggcgacgt ggcggatgtc cagcggcagc
      421 ccgaggaggt gtccgaaata gccggtcata cccaagagca tcccgaagat gaagttgcct
      481 gccaacgagc cgtagtgttt gtggatgtag gcggcgagga cgcggcgggg cccggggcgc
      541 atgattttac gcagcaaggg gttgaagggc aggcgttggc gcaggttgag gtagtcggcg
      601 cggttgtcga aaaaacctgc gatgatgccg gagcagaaca gccacagacc tgcaatggcg
      661 gcataccaca gcgtcgggta agcgatgatg tctatggatt tgaactggta ggcggcggtg
      721 tgggcatcga gtatgggctg ccggtacaga tgggcatagc cgaacgatat ggcgcacgcc
      781 aaaaggatgg cgatggaaac gttgccgaaa acggcgacac tttgggaacg gcatacgtcg
      841 atgaggagct tggcgagttt gttgtccacc gctttgccgc cttcgttgag atcgacctgt
      901 tcggcaaagc tggcggcagt catcgcgggc tgtttggtgg cgacggtgca gtgcagcata
      961 tggatgatca taaagccgat gccgtagttg aacccagccg acaaggaagt caggaagggg
     1021 ctgaggccga gtgtgccgat gcggattttg agcagcgcca tcagggcgat gatgatgccg
     1081 ccgcctgccg ccgagtagaa cattttgaaa tattctttgc ggttgcgggt aatgtagtgt
     1141 tcgccgtggt ggcttttgtt ttcggtaatg ctgcgggcga gcatacggat gctgctgcgg
     1201 cgcaggtggc gggtgctgta ttgttcgacc gccgcgtaga tgagggagtt catcagcgcg
     1261 atggtcaggc ggttgctgtt gccggcgccg gtttggatgt cggtgagcag cttcagacgg
     1321 tctacggtct gccggagccg ttcgagcagg tgggcgactt tgaccgacga accggagccg
     1381 gcgcccgtcc ctttgcggcg caaatagtca atctggctga aacattggtc gaacatcact
     1441 tcgaggtggg cggtgtcgta aggcgtggtg ccgttgcggt agtgttcgac cagtttggcg
     1501 gtttcgcgtt ggagggcgac gaaggaagaa tcggcttcca gcaggcgcgg ggcgatgcgg
     1561 atgaggtcgg gttcgatggc ttcggatgcc gtccagacag acagcatttc gatggcgcgc
     1621 aaacgcgcat cggcaaggcg gatgcccgcc gtctgcaatt tggacggctc ggcgtggctc
     1681 cggatgagtt cgtataaaac cagccattgg cggatattga gcgtttgcag ccatttgtcg
     1741 tcgttttcgg aatggaaaag ataaaggaag acttcgccca agttggcaaa atctttatat
     1801 gacgggctga agcgttcgta gatgcgtatg cccatttccc gggcaaagct gtggcgcgag
     1861 aagatgccga gtttgatgag tgccggataa atgtgtattt gcgcgagcca gatgtaaaaa
     1921 caccggctga agcgggacag caattccctg tcttgtttga atgtgtcgat aatcaggtcg
     1981 aaacgttccc ccgcacattt tttgccgcca cggcgcaaaa atttaatcag tgcgttgagg
     2041 acgttgacaa aatcggtatg tcccaagctt tccgaaagca gggggcgcag gttttgaggg
     2101 gtaattttct tcatatcagc cattgtaccg catcggcaat ccggcgttaa acgccccgag
     2161 cggggagggc gcgcctgcgg aaggcggcgc attttccgcc ggtttgaaat caaactatat
     2221 taatgatata ataaatatcg ataaatcaat ctgatagaat aatccgtatt gatttttaat
     2281 tttttcgatt ataacaattt cggccggttt gtgcatttcg tcatccggaa gccggtattt
     2341 tgccggcagg ttttgaccgc tttgcccgat gaccgggttt gtctgttttt aacaaaggag
     2401 tgagaaggtt tgtccctgtc tgaatttata gaacgccgaa cgtcatttaa tctgatggtt
     2461 attttgacga ctttgttttt tgtgtgtgtt ttggtggtat tggttttaac cgtgccggat
     2521 caggtgcaga tgtggctcga tcgggcaaaa gaaatcattt ttaccgagtt cagctggttt
     2581 tatgttttaa cgttttccat ttttctgggt ttcctgctga tactctcggt cagcagtttg
     2641 ggaaacatca ggctcggacg ggatgaagat gtgccggaat tcggcttcct gtcgtggctg
     2701 gcgatgctgt ttgcggccgg gatgggcgtg ggtctgatgt ttttcggcgt ggcagagccg
     2761 ttgatgcatt atttttcgga cattacggcc ggcacgccgg aacacaggca gcagcaggca
     2821 ttgctgcaca cggtgttcca ttggggcgtt cacgcttggt cggtgtacgg tacgattgca
     2881 ttggctttgg cttatttcgg tttccgctac aagctgccgc ttgccctgcg ttcttgtttt
     2941 taccccctgt tgaaagaaaa aatttccgga aggttcggcg atgccattga tattatggcg
     3001 ttgcttgcta cttttttcgg catcatcacc acattggggt tcggggcttc gcaactgggc
     3061 gccggattgc aggaaatggg ctggattgcc gaaaacagct tcagcgtgca ggttttgatt
     3121 atcgccgccg tcatgtccct cgccgtcgtt tcggcaatat ccggcgtggg gaagggcgtg
     3181 aaggtgttga gcgagttgaa cctgagcctt gcgtttttgc tgctgttttt tgttttggcg
     3241 gcgggtccca ctgtttacct gttgtcggca ttcggcgaca acatagggaa ctacctcgga
     3301 aatctggtgc gcctcagttt taaaacttat gcgtacgaac gggaacacaa gccgtggttt
     3361 gaatcttgga cggtgcttta ttgggcgtgg tggtgttctt gggcgccgtt tgtgggtttg
     3421 tttatcgcgc gcatttcaaa ggggcgcacc atccgcgagt ttgtcttcgg ggttttgctc
     3481 atccccggcc tgttcggcgt tttgtggttt accgtcttcg gcaatacggc gatttggctg
     3541 aatgacgggg ttgcgggggg aatgctcgaa aagatgacct cctctccgga aacgctgctt
     3601 tttaaattct ttaattacct ccccctgccc gaattgacga gcatcgtcag cctgctggtc
     3661 atttctctgt tttttgtaac ttctgccgat tccgggattt atgtcctgaa caatattacc
     3721 tctcgggaca aaggcttgag cgcgccacgg tggcaggcgg ttatgtgggg cgtgctgatg
     3781 tctgccgttg ccgttttgct gatgcgctcg ggcggactcg gcaacctgca gtctatgacc
     3841 ctgattgttt ccctgccgtt tgccctgctg atgctgataa tgtgtttcag cctgtggaaa
     3901 ggcttgagtg cggataagaa atattttgag acccgggtta accctaccag tgtattttgg
     3961 acgggcggca agtggaaaga acggctggtg cagataatga gccagacgca ggagcaggat
     4021 attttaaaat tcctcaaaca taccgcatcg cccgctatgc acgagttgca acgggagctt
     4081 tcggaagaat acggcttgag cgtccgggtc gataagatgt ttcatcagga cgagcccgca
     4141 atcgagttcg tcattcggaa agagacgatg cgcgatttta tgtacgggat taagtctgtc
     4201 gggcaggatg tatccgacca gttgattaac gacggcaagc tgccgcatat ccggcatcag
     4261 acaacttaca aaccctacgc ttattttttc gacgggcgcg tcgggtacga tgtgcagtat
     4321 atgaacaagg acgagctgat tgccgacatt ttgaaaaact acgaacgtta tttgatgttg
     4381 ttggatgatg tcggtcagga actgatggcg cacgagcagg tggaattggc agagtaagtg
     4441 ccttatctct ggcgtttgcc aaaaaaaatg cggtgaatca gattcaccgc attttttttg
     4501 ccggacgggg cttcggacgg cacggcgttc atttgtttcc gtcaaactgc tccttcaaca
     4561 cccgtttcaa taccttgccc gtagcgttgc gcggcagccc gtctttaaag tggatttgtt
     4621 tggggatttt gaaatttgcc agcacggtac gcaggtggcg gcggatttcg ttctcgccca
     4681 aatccatacc ttccttcaat tggacgaagg cgacgatttc ctcgtcggca taacggtctt
     4741 tcacgccgat gacggcggcg gcttcgacgg catcgagttt gtagatttct tcttcgattt
     4801 cgcgcggata gacgttttga cctttggaaa taatcaaatc ttttttgcgg tcgacgataa
     4861 agataaagcc gtcttcgtct atggtaacga aatcgcccgt tttcaaccag ccgttgacga
     4921 tggtttcatc ggtggcggca ggcatattga ggtagccccg catcaccgaa ccgcccctga
     4981 cgatcagttc gcccacttcg ccgcgcggca cttcgaccaa ttcttcatcg acggctttgg
     5041 cttccaaacc gggcaggggg atgccgacgc tgcgggcttt ttgcctctcg ggcgtattga
     5101 cggcgacgac gggcgaggct tcgctcagtc cgtagccttc cagcaatttg gcgcggggga
     5161 acttggcttt gaaatcgagg atggtttgtt ccgccaaagg tgctccgccg ctgataaaca
     5221 ggcggatgcg gttgaaccat ctgaaatacc aagggatttt cgtcttgctc atcgcggtgt
     5281 aaatcgcggg cacgcccaaa aacacggtcg cgcgtttgag cagtgcctgt ttcaaaacgt
     5341 tggaaaaggg gaaaacggat ttgaccaaaa taatcgaaca cgccatataa atcggcagca
     5401 gcaccatagc cgtcagcgtg aagctgtgga acatcggcag gaaaacgata aagcggtcgc
     5461 gcttggagat tttaaagatg cgttcgatgc cgttcaggtt ggcgaacagg ttggcgtagc
     5521 tgattagcgc acctttggga tgccccgtcg tgccggaggt gtagatgatg tgtgccaaat
     5581 catttatccg gggttggcgg cccaagtcgg gtttttcggg gaagcggcgc acgtcttcaa
     5641 aaaaggcatc gccttccgcc atttcgccgt ccggacggct ttggcccgtc caaatgattt
     5701 tttcgacggg cgtttgcgcc ttcaagcccg ccaattcttt tgacaggccg gccgaggcga
     5761 acaggaagcg cgccttgcag tcgttcagga tatacgcgta ttcgctgttt ttcaaaaatg
     5821 tgttcatcgg tacggcaacc gcgccgatgg cggatacggc gaaataggcg gtaataaatt
     5881 ccgtggaatt ggaaaccgcc agcgcgaccg tgtcgccgaa cttcacgccg atattttgca
     5941 gatacgccgc aacggcttcg gcttcctgct tgagcgcgcg gtaggcggtt ttttccttgc
     6001 cgtcgaacac tgccgtgccg ttgccgtttt tgcggcaggc ggcggtcagc atttcgtaga
     6061 aattggcata agtccggttc atgaaacttc ctttatggtt gcggacggcg aacgccctca
     6121 tttgaaaaca atatgccg
//
LOCUS       DAWKOG010000120         6117 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.120, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000120 DAWKOG010000000
VERSION     DAWKOG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6117
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.120"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(151..2079)
                     /gene="dnaK"
                     /locus_tag="WJK66_001614"
     CDS             complement(151..2079)
                     /gene="dnaK"
                     /locus_tag="WJK66_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEZ7579303.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDHIIAEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKAEALGTASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
     gene            complement(2287..2559)
                     /locus_tag="WJK66_001615"
     CDS             complement(2287..2559)
                     /locus_tag="WJK66_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579304.1"
                     /translation="MKKTGKYLIYAAAFTAFCFAFQENRSEAKQPDITLSASLCEKFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            2745..3431
                     /locus_tag="WJK66_001616"
     CDS             2745..3431
                     /locus_tag="WJK66_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7579305.1"
                     /translation="METFKDRLVFLWKSEAKQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            3563..3901
                     /gene="erpA"
                     /locus_tag="WJK66_001617"
     CDS             3563..3901
                     /gene="erpA"
                     /locus_tag="WJK66_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEZ7579306.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            3918..4064
                     /locus_tag="WJK66_001618"
     CDS             3918..4064
                     /locus_tag="WJK66_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579307.1"
                     /translation="MPSERSDGIFTFRKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            4061..4525
                     /locus_tag="WJK66_001619"
     CDS             4061..4525
                     /locus_tag="WJK66_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ7579308.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPQVDRGLIRECLKENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWAKREELHDYFSYLGLDIITRRYMELETVVPPEEGEGEGEG
                     ADGEMPEYLELHGGREDDISAPSQPEPPTRNIKPLLFGLLIAFLGYLLGKIF"
     gene            4560..6071
                     /gene="ubiB"
                     /locus_tag="WJK66_001620"
     CDS             4560..6071
                     /gene="ubiB"
                     /locus_tag="WJK66_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEZ7579309.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQSIEKLYAEFETEPIASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAAWVERLFSDGRRLKPLEVVAEFGKYLHDELDLMREAANAGQLGRNFHNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVADIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAVIAVVLLLILLLK"
BASE COUNT         1374 a         1358 c         1637 g         1748 t
ORIGIN      
        1 aaaatttcca gagaaccgat acaacagttg gaacttgggt ttgggaatat tacggtagat
       61 gaacttggaa cctctgttat gctatggtct tttatctcaa ttgaaaaaag cgcgaatcaa
      121 acggttcgcg cttttttcag acggcatcaa ttattttttg tcgtctttta cttcttcaaa
      181 gtcggcatct acgacatcat cgtctttctt tgcagaagca ttggcttgtt cgctttcgcc
      241 tgcttgggct tcagcttgtg cttgagcgta aaccatttcg cccagttttt ggctggctgt
      301 gcccagcgcc tcggctttgg catcgatggc ggctttgtcg tcgcctttca cggcttcttc
      361 ggcttctttc agcgcggctt cgattttttc tttttcggca gcgtcgagtt tgtcgccgta
      421 gtccgccaaa gattttttca cggagtgaat cagggcttcg gcttggttgc gggaagcgac
      481 caattcagtc agctttttat cttcctcagc attggcttcg gcatctttca ccatgcgttc
      541 gatttcttct tcgctcaaac ctgaagaacc ttggatggta atgttggctg ctttaccggt
      601 gcctttgtct ttggcggaaa cgtgcaggat gccgttggcg tcgatgtcga aggttacttc
      661 gatttgcggc ataccgcgcg gtgcaggtgc gatgtcgccc aagttgaact gacccaaaga
      721 tttgttggca gaagcgcgtt cgcgttcgcc ttgcagtacg tggatggtta ctgcgctttg
      781 gttgtcttcg gcggtagaga acacttgcga cgctttagtc gggatagttg tgttcttttg
      841 aatcagcttg gtcatcacgc cgcccatggt ttcgataccc aaggacagag gggttacgtc
      901 cagcagcaat acgtcgctgc ggccgccgct taacacttcg ccttgaatcg ctgcgcctac
      961 ggcgacagct tcgtcagggt tcacgtcttt gcgcggctct ttgccgaaga agtctttaac
     1021 ggcttcttgt actttcggca tacggctttg accaccgacc aaaatcacgt cgtcgatgtc
     1081 gccagtgctc agaccggcat ctttcaatgc ggtgcggcaa ggctcgatag agcgggcaat
     1141 caggtcttca accaggcttt cgaatttggc gcgggtaatt ttcatcgcca agtgtttcgg
     1201 gccggttgcg tccatggtga tgtacggcag gttaatttcg gtttgctggc cgctggacaa
     1261 ttcgattttg gctttttcgg cagcttcttt caggcgttgt agagccatca cgtcttgttt
     1321 caaatcaatg ccttgttctt ttttgaactc ggcgatgatg tggtcgatga ggcgttggtc
     1381 gaagtcttca ccgcccaaga aggtatcgcc gttggttgcc aatacttcga attgtttgtc
     1441 gccgtcgagg ttggcgattt cgatgatgga aatatcaaaa gtaccgccgc ccaagtcata
     1501 tacggctact ttgcggtctt tgttgtcgcc tttgtccata ccgaatgcca aagcggctgc
     1561 ggtcggctcg ttgatgatgc gttttacgtc caaaccggcg atacggcctg cgtctttggt
     1621 ggcttgacgt tggctgtcgt tgaagtaggc cggaacggta atcacggctt cggttacttt
     1681 ttcgcccaag taagcttcgg cggcttcttt cattttacgc aggacttctg cggaaatttg
     1741 aggaggagac agctctttgc cttgcgcttt cacccatgcg tcgccgttgt tggctttgat
     1801 gatttcaaaa ggcatagatt cgatgtcgcg ttggacttct ttgtcttcaa atttgtggcc
     1861 gatcaaacgt ttggcggcgt aaatagtgtt tttagcgttg gttacggctt ggcgtttggc
     1921 aggcgcgccg acgaggattt cgccgccgtc caaataagcg ataacggacg gcgtggtgcg
     1981 tgcgccttct gcgttttcga tcactttggt ttgaccgttt tcggaaatgg ccaaacaaga
     2041 gttggttgta cctaagtcga taccgattac ttttgccatg tggataatcc tatttgattt
     2101 tgcttatttt gagaaatatg ttggaacatt ttgtcccgat gggctgtaaa tagggcgggc
     2161 ggcgggctgt ttcaagctac agcatggcta taagtatata actttatgaa tatattggtt
     2221 ttatatttga tttaatacat ttggctccaa tgcattcaag cataatgttt caaatggcag
     2281 gcaggtttat tcatagacga tgccggcgag catttcctgt tcgttcaagt tgccgtactc
     2341 tttttcccag tcgtgtgaag actcgatgat gtcgcattct ttggaaaggg agacttgttc
     2401 tgcatccata tctttggcgt tcagtatgtt gaatttttcg cacagggatg cggataaagt
     2461 gatgtcaggc tgtttggctt cagaacggtt ttcttggaag gcaaagcaga atgcggtaaa
     2521 tgccgcagca tagataagat atttgccggt tttcttcatt ttctatcctt tttctgtcaa
     2581 cttaggatta aacctatgga aaatcttaaa aattatgtat tgagtaagaa aaatcataat
     2641 ttaaatttag tttatcatag ttgttccgtt tttgggatag ctaaggtaaa atatatttca
     2701 tgtttacttt agatgattga atgaagggga gcggaaggat atttatggaa acctttaaag
     2761 acagactggt ttttttatgg aaaagcgaag cgaagcaggc aaaaatcgca tccgatattg
     2821 aaatgacgat tgcgggcttc agcaggatat ggaatgaagg cggtctgccg aagtctgaaa
     2881 cattaaaaaa aatcaagcag ttgaaggggt gtagtatcga ttggctgctg accggggagg
     2941 gtaatccgtt tccggatgaa gccccaaaaa aatcccttgc ttacgatact ttgggcaatg
     3001 aagtcgatac ggacgagttt gtcttcgtgc cgagatatga tattcgggcg gctgcgggat
     3061 acgggcagtt tgtcgatcat gaggaaccgg tatttacaat ggcgttcaga cggcattgga
     3121 ttgagaatta tgttacccgc gatacgaaaa acctgtctgt aatttccgtc aagggggatt
     3181 cgatggaggg ggttttgaat gacggcgatt cgattttggt caatcatggt gaaaatacgc
     3241 cgagggacgg tctgtatgtg ttgcggatta atgaaaatct gctggttaaa cgtttacaga
     3301 ttgtaccggg cgggattatc aatgtgattt ctgcaaacga ggcttatcct gcttttgaaa
     3361 tcaatttgaa cgatttgacc gatgatgtgg agattatcgg gcgtgtcgag tggttcggca
     3421 ggacgatttg agtttggggc ttgaaattgc aggcggtcaa acttatctat tggaacaatt
     3481 cctttttcaa aggcgaagcc tgcttgcctt tgaagggggt ttgagagaga atgcagaaaa
     3541 tattatatta aggaataaca ccatgtcgga tgaaagccct attattttta ctgacagctg
     3601 ctgtgccaaa gttgccgatt tgattgccga agaaaacaat cccgatttga aattgcgggt
     3661 ttttgtcaat ggcggcggct gttcgggttt ccagtacgga tttacttttg acgaaatcaa
     3721 aaacgacgac gattttgaaa ttgagaaaaa cggtttggtc tttttggtcg atccgatgag
     3781 ctatcaatat ctggtcggtg cggaaatcga ctatacggaa agtttgcagg gttcgcaatt
     3841 cgtcatccgc aatccgaatg cggaaacaac ctgcggttgc ggatcgtcgt tttccgtatg
     3901 accgcttggt ttgtgtgatg ccgtctgaac gttcagacgg catttttact tttagaaaat
     3961 atattatcgg gatgaattca catataatcc gattgtttga agatgaatcg ggtttcccga
     4021 aaggaacggg cggaacggta tcaggcgtat ttgttccctt atgattgaga tgagtaaaga
     4081 ttaccgaaac gatttgtacg atgtatatgt ttcttacccg ccccaagtgg atcgcgggct
     4141 tatccgggag tgccttaagg agaatctcgg cgaggaaaag gcggaaggat tgatcgaatc
     4201 gctcgattcc aaacctcaag tgctggttga ggaaaaatgc acttgggcga aacgggaaga
     4261 gttgcatgat tatttcagct atttgggttt ggatattatt acccggagat atatggagtt
     4321 agaaacggtc gtgccgccgg aggaaggtga gggcgaagga gagggcgcgg atggggaaat
     4381 gcccgaatat cttgaacttc atggcgggcg ggaagatgat atttccgcac cttcgcaacc
     4441 cgaaccgccg acccgcaata tcaaaccgct gcttttcggg ctgctgattg cctttttggg
     4501 ctatctgctc ggtaagattt tttgattgtc cgataaatgc tgtattcggg attttatata
     4561 tgaaatggtt gaaacgcctg acggtcattg tcgggacttt ttaccgctat cggctggcag
     4621 gtctgtgtgc ttcgctgatg ggtagcggtt ggatatgcgc tctgctgaaa atgatgccgc
     4681 agtcgtccaa attgaaaaac gaaccgcctg ccgtccgtct gcgccttgcc ttggaaagcc
     4741 tggggccgat tttcatcaag ttcgggcagg ttttgtccac acgccccgat ttgattccgc
     4801 acgattacgc cgtcgaactg gcaaagctgc aagacaaagt gccgcctttt gacgcgcggc
     4861 tttcgcgtga acaaatcgaa aaatcgttgg gtcagtccat cgaaaagctg tatgcggaat
     4921 ttgaaaccga gcccatcgcc agcgcgtcca tcgcccaagt acacaaagcc cgcctgcatt
     4981 cgggcgaacg agtggcggtt aaagttttgc gccccaacct tttgcccctt atcgaacagg
     5041 atttgtcgct gatgcgtttc ggcgcggcct gggtcgaacg cttgttttca gacggcaggc
     5101 gtctgaagcc gctcgaagtg gtggcggagt tcggcaaata cctgcacgac gagttggatt
     5161 tgatgcgcga agccgccaat gccggacagc tcgggcgcaa tttccacaac agcaatatgc
     5221 tgattgtgcc caaggtgttt tacgactact gcaccagcga cgtgctgacg attgaatgga
     5281 tggacggtac gccggttgcc gacatcgcca aactcaaagc agacggcatc gatttgcaca
     5341 aactcgccga ttacggcgtg gaaatctttt tcacgcaggt cttccgcgac ggctttttcc
     5401 acgcggatat gcaccccggc aatattttgg ttgccgccga caaccgctac atcgccctcg
     5461 atttcggcat cgtcggcacg ctgaccgatt acgacaaacg ttatctcgcc atcaatttcc
     5521 tcgccttttt caaccgcgat taccggcgcg tcgccaccgc ccacatcgaa tcgggctggg
     5581 tgcctgccga cacgcgtgcg gaagaattgg aagccgccgt ccgcgccgtg tgcgaaccgg
     5641 tgttcaacaa accgatttcg cagatttcct tcggcttggt gctgatgcgc ctgtttgaag
     5701 tcagccgccg cttcaatgtc gaaatccaac cgcagcttgt gctgctgcaa aaaacgctgc
     5761 tcaacatcga aggcttgggc cgccagctcg atcccgattt ggacttgtgg aaaaccgcca
     5821 aaccgttttt ggtgaaatgg atgaacgggc aggtcggccc taaagccctt tggcgcaacc
     5881 tcaaaaacga agcccccgac tgggcgcaaa tcattccttc attgccgcgc aaaatcagtg
     5941 cgttgattga tgaaaaccgc cagcaggaaa tgcgtgatgc ctatatccat ttggtcaaag
     6001 tgcagcagcg gcaaagcctg tggctggctg tgattgcggt tgttttgctg ctgattttgc
     6061 ttttgaaata ggctttgtcc gaatcatcgc ccgactccgc ccgtttataa ggaaatc
//
LOCUS       DAWKOG010000121         6108 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.121, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000121 DAWKOG010000000
VERSION     DAWKOG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6108
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.121"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            20..403
                     /locus_tag="WJK66_001621"
     CDS             20..403
                     /locus_tag="WJK66_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEZ7579310.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKLA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
     gene            518..991
                     /locus_tag="WJK66_001622"
     CDS             518..991
                     /locus_tag="WJK66_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEZ7579311.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMRIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVEGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKIAPMPAMNHGHHHGEAHQH"
     gene            1117..2220
                     /gene="mnmA"
                     /locus_tag="WJK66_001623"
     CDS             1117..2220
                     /gene="mnmA"
                     /locus_tag="WJK66_001623"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEZ7579312.1"
                     /translation="MNTTANTTNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIIGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFNLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDDETAELVFDEPQW
                     AVTPGQSAVLYDGDICLGGGIIQTTDKPVIITR"
     gene            2291..3961
                     /locus_tag="WJK66_001624"
     CDS             2291..3961
                     /locus_tag="WJK66_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEZ7579313.1"
                     /translation="MKKIWLDSYEKGVSAEIDITQYNSVSDVFRQSVEKFADQPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMLPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFIIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIILITNPRDMKGFIGQLKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITDTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLIVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVRKDPS
                     LTKEELIAFCRTELTAYKVPKDIEFRDELPKSNVGKILRRELRQSTGK"
     gene            4073..5707
                     /locus_tag="WJK66_001625"
     CDS             4073..5707
                     /locus_tag="WJK66_001625"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEZ7579314.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDREMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAVKNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NKGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAAKIYGSGHIRERHRHRYEVNNNYVPQLEKAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
     gene            5722..5924
                     /locus_tag="WJK66_001626"
                     /pseudo
     CDS             5722..5924
                     /locus_tag="WJK66_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246312.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5935..6108
                     /locus_tag="WJK66_001627"
                     /pseudo
     CDS             5935..6108
                     /locus_tag="WJK66_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         1599 a         1647 c         1536 g         1326 t
ORIGIN      
        1 acattttgaa aggataccga tggaaccttc ttcttacgcg gcagaaaaaa aaggcaaagg
       61 cggcatcagg cgcgtcatta acgcattcgg ctattcgata gacggcatcg ccgccgccta
      121 ccgttacgaa gcggcattcc gtcaggtttt gtggctgaac gtactgctgg tgtgcgcggc
      181 atttttttgg gtttccgaaa ccgccgtccg cctgccgttg attatcgcgt cttttgtgtc
      241 ggtcattgtc gaactgttca acaccgccgt cgaagctgcc gtcgatcata cttcgaccga
      301 aaaacacgag ctggccaaac tcgccaaaga cgcaggttct gccgcacaat tgttcgcgat
      361 gctgatgttg gcggcggttt ggctgtctgc cctgttcggg taaaacgctt gcagcaggat
      421 ttgtaatcct ttaggattag tatgtggtat ttattattca cttaatccgc tataagatta
      481 agcacattcc ccgtctgata ttataaggag cagaaagatg aaaaaattat tggcggccgt
      541 gatgatggca ggtttggcag gcgcggtttc cgccgccgga gtccacgttg aggacggctg
      601 ggcgcgcacc accgtcgaag gtatgagaat aggcggcgcg ttcatgaaaa tccacaacga
      661 cgaagccaaa caagactttt tgctcggcgg aagcagcccc gttgccgacc gtgtcgaagt
      721 gcatacccac atcaacgaca acggcgtgat gcggatgcgc gaagtcgaag gcggtgtgcc
      781 tttggaggcg aaatccgtta ccgaactcaa gcccggcagc tatcacgtga tgtttatggg
      841 tttgaaaaaa caattaaaag agggcgacaa gattcccgtt accctgaaat ttaaaaacgc
      901 caaagcgcaa accgtccaac tggaagtcaa aatcgcgccg atgccggcaa tgaaccacgg
      961 tcatcaccac ggcgaagcgc atcagcacta atctgctgaa aatgtttgaa atgccgtctg
     1021 aaaaagcccg ggctttcaga cggcattttt atgcccgcct ttaaaatgtg ttaaaatccg
     1081 cctttaaaaa ccgccgtttc caaccatatt ctacgcatga atacgacagc aaacaccacc
     1141 aatatcatcg tcgggctctc cggcggtgtc gattcttccg taaccgccgc cctgctcaag
     1201 cagcagggtt atcaagtgcg cggtgtgttc atgcagaact gggaagacga cgacaacgac
     1261 gaatattgca gcatcaaaca ggattcgttc gatgccatcg ccgttgccga tattatcggc
     1321 atcgatatcg acatcgttaa tttcgccgcg caatataaag acaaagtctt tgcttatttt
     1381 cttcaggaat acagtgcggg gcgcacgccg aatccggatg tgttgtgcaa cgccgaaatc
     1441 aaattcaaat gctttttgga ctacgccgta gggcagggcg cggataccat cgccaccgga
     1501 cactatgcgc gcaaagaagt ccgcaacggc gtgcattacc tgctcaaagg tttggatcga
     1561 aacaaagacc aaagctattt cctctaccgc ctcaagcctt tccaactcga gcgcgcgatt
     1621 tttccgttgg gcggtttgga aaaacccgaa gtgcgccgcc ttgccgccga atttaatttg
     1681 ccgactgccg ctaaaaaaga cagcacgggc atctgtttca tcggcgaacg cccgtttcgc
     1741 gagtttctgc agaaatacct gccgaccgac aatggtaaaa tggttacgcc cgaagggaaa
     1801 accgtcggcg aacacgtcgg gctgatgttc tacacgctcg gacagcgcaa aggtttgggc
     1861 attggcggcg cgggcgaacc gtggtttgtt gcggctaaag atttgacgaa aaacgaactg
     1921 attgtcgtgc aaggacacga ccatccgctg ctttataccc gcagccttgt gatgaacgat
     1981 ttgagtttca cgctgcccga acgcccgaag gcaggacgct atacctgcaa aacgcgttac
     2041 cgtatggcgg acgcgccttg tgagttgcgc tatttggatg atgaaaccgc cgagctggtg
     2101 tttgacgaac cgcaatgggc ggttacgccg ggtcagtccg ccgtgctgta cgacggcgat
     2161 atctgtttgg gcggcggtat catccaaacg accgacaaac ccgtcatcat cacacgataa
     2221 aggtaatgcc gtctgaaacg gttttcagac ggcattgttc cgctcaattc cactttaaag
     2281 accgatacct atgaaaaaaa tttggttaga cagctacgag aagggcgtca gtgccgaaat
     2341 cgacatcacg caatacaatt ccgtcagcga cgtattccgc caaagcgtgg aaaaatttgc
     2401 cgaccaacct gcttttcaaa acatgggtaa gacgctgact tatgccgaaa ccggcaaact
     2461 ggcaactgat ttcgcctctt atctgcaaaa cgtcctcaag ctgccgcgcg gcgaacgtgt
     2521 tgccattatg ctgcccaacg tattgcaata cccgattgcc cttttcggta ttttgcaggc
     2581 aggtttggtg gcggtgaaca ccaatccgct ctatacaccg cgcgagttgg agcatcagtt
     2641 gaaagacagc ggtgcgaccg ccatcatcgt tttggaaaat ttcgccaaca cgctggagct
     2701 ggtgctgccg cgcacgcaga tcaaacacgt catcgtcgcc tccgtcggcg aaatgttcgg
     2761 gctgcttaaa ggttcgctga tcaatttcat catccggaaa atcaagaaaa tggtgcccga
     2821 ataccgtatt cgggaaaccg tttcctttca gacggcattg aaagaagggg cgaagcacgt
     2881 tttccaacct gtcgcattaa accgcgaaga taccgcgctg ttgcaataca cgggcggcac
     2941 gacaggtgtt gccaaagggg cggtgctgag tcacggcaac atctgcgcca atatgcttca
     3001 ggcaaaagaa tggattaaaa accagttgcg cgagggcaaa gaaaccgtta tcgccgcctt
     3061 gccgctgtac catattttcg ccttaaccgt gaatctgatg atttttgcca atgccggctc
     3121 gaaaatcatc ctgattacca acccgcgcga tatgaaaggc tttatcggac aactgaaaaa
     3181 acagcgggtt aatgtattta tcggcgtgaa cacgctgttt aacgcgatgg ttaaccgtcc
     3241 tgattttgcc gaagtcgatt tttcaggatt gcggctgact ttgggcggcg gtatggcgac
     3301 ccaaaaagcc gttgccgaaa aatggaaaaa aatcaccgac acgcccatcg tcgaagccta
     3361 cggtctgacc gaagccagcc ccggcgtgtg ctgcaacccc ttaaacatcg aatcatacag
     3421 cggcagcatc ggtttgcccg tctcgtccac cgaagtcgaa ctgcgcgacg caaacggcaa
     3481 agaagtcccc gtcgggcagc cgggcgaatt gtgggtaaaa ggccctcaag tgatgcaagg
     3541 ctactggaac cgtcccgaag aaaccgctaa agccatagac gcgcgcggtt tcttggaaac
     3601 cggcgatatt gccgtcatgg acgaaaaagg ctggttgaag ctggttgacc gtaagaaaga
     3661 cctcattgtt gtttccggat tcaatgttta tccgaacgaa atcgaagaag tcatcgcgca
     3721 tcacggcaaa gtgatggaag ttgcctgtat cggcgttccc gacgaaaaaa ccggcgaggc
     3781 actcaaagta ttcgtcgtca gaaaagaccc gtctttgacg aaagaagaac ttatcgcctt
     3841 ttgccgcacc gaattaaccg catataaagt accgaaagat atcgaattcc gcgacgagtt
     3901 gcccaagtcc aacgtcggca aaatcctgcg ccgcgagttg cggcaaagta ccgggaaata
     3961 aagaaaagat gccgtctgaa aacagccgtc caccgttcag acggcatccg tccgtttgcc
     4021 agaaccgcgc gcttcacgtt aaaatcacgc attccaatat gggtattcca tcatgaccaa
     4081 attcattttc gtaaccggcg gcgttgtctc ctcactgggt aaaggtatcg ccgccgcttc
     4141 tattgccgcc atcctcgaat cgcgcggctt gaacgttacc atgctcaagc tcgatcctta
     4201 tatcaacgtc gatcccggca cgatgagccc gttccaacac ggcgaagtgt tcgtaaccga
     4261 cgacggcgcg gaaaccgacc tcgacttggg acactacgaa cgctttatcg attccacgat
     4321 gacccgccgc aacagcttca gcacggggca ggtgtacgaa aacgtcatcg ccaaagaacg
     4381 acggggcgac tatttgggcg gcacggttca agtcattccg cacattaccg acgaaatcaa
     4441 acgccgcatc cacgaaggcg cggcaggtta cgatgtggcg attgtcgaaa tcggcggtac
     4501 agtcggcgat atcgaatctt tgccattctt ggaagccatc cgccaaatgc gcagccagct
     4561 cggccgcaac aatacattgt ttgcccacct gagctatgtt ccttacattg ccgccgccgg
     4621 tgaaatcaaa accaaaccga cccagcatac cgttaaggaa atgttgagca ttggtttgca
     4681 accggacatc ctgatctgcc gtatggacag agaaatgcct gcggacgagc gccgcaaaat
     4741 cgccttgttc tgcaacgtgg aagaacgcgc gattgtgggc agctacgacg tggacagcat
     4801 ctacgaatgc cccgaaatgc tgcacgacca aggcatagac aacatcatta ccgaacagtt
     4861 gcagcttaac gtacaacagg cggatttgac tgcgtggaaa aaaatcgtcc acgccgtcaa
     4921 aaatccgaaa cacaccgtca aaatcgcgat ggtcggcaaa tacgtcgatt tgaccgagtc
     4981 ctacaaatca ttgattgaag ccttgaaaca cgcgggtatc cacaccgaaa ccgacgtgca
     5041 aattaccttt gtcgacagcg aaagcatcga gaaaaacaaa ggcgacgttt ccatgctcaa
     5101 agatatggat gccatcctcg tccccggcgg tttcggttcg cgcggcgtgg aaggcaaaat
     5161 cgccgccgtg cgctacgccc gcgaaaacaa cgtgccatac ttgggcatct gcctcggtat
     5221 gcagattgcg ctgatcgaat acgcccgcga cgtggcaggt ttgaaaggtg cgaattccac
     5281 cgagttcgac ctcaaatgcg ccgcccccgt cgttgccctg attgacgaat ggcaaactgc
     5341 cgacggcagc gtcgaaaccc gagacgagtc cgccgatttg ggcggcacga tgcgtttggg
     5401 cgcgcaagaa gtcgaattga aagcaggcag cctcgccgcc aaaatctacg gcagcggaca
     5461 tatccgtgaa cgccaccgcc accgctacga agtcaacaac aactatgttc ctcagttgga
     5521 aaaagcaggc ttggttatcg gcggcgtatc cgccggacgc gaacgcttgg tcgaaaccat
     5581 cgaactgccg aaccatcctt ggttcttcgc ctgccagttc caccccgagt ttacctccaa
     5641 tccgcgcaaa gggcatcctt tgttcaccgc gtttgtcaaa gctgcgttga acaataaaaa
     5701 agcctgataa agcgttactt gatgatcaaa atgccgtctg aaagcctgaa tgaagacttt
     5761 cagacggcat tttcgcaaat cggggattat aaaagccatc aatttaattt ttatggtttt
     5821 atctaatatt taccaactga atattgttaa gcggggcgtg aaactcaagt tgagaccttt
     5881 gcaaaattcc ccaaaatccc ctaaattccc accaagacat ttaggagatt tctcatgagc
     5941 accttctttc agcaaaccgc acaagccatg attgccaaac acatcgaccg cttcccacta
     6001 ttgaagttgg accaggtgat tgattggcag ccgatcgaac agtacctgaa tcgtcaaaga
     6061 acccatttac cttagagacc accgcggccg tcccgcctat ccgctgct
//
LOCUS       DAWKOG010000122         6085 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.122, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000122 DAWKOG010000000
VERSION     DAWKOG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6085
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.122"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(83..1276)
                     /locus_tag="WJK66_001628"
     CDS             complement(83..1276)
                     /locus_tag="WJK66_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ7579315.1"
                     /translation="MYYYDEYSGSRTPVYVDVYELDEKGNKIQETNPDGTPAFTGFSG
                     TVPVWKTVKVADDHVPALYNYAKYLNTNKTHSLTAGTRFNVTGRLHLLGGLHYTRYET
                     SQTKDMPVRYGQPASDFQTASSIKADQDHYTAKMQGHKLTPYAGITYDLTPQQSIYGS
                     YTKIFKQQDNVDVSAKTVLPPLVGTNYEVGWKGAFLQGRLNASFALFYLEQKNRTVVD
                     FGYVPGAGGKQGSFQTVAKPIGKVVSRGAEFELSGELNEDWKVFAGYTYNKSRYKNAA
                     EVNAERLAKNTGADPYNFSNFTPVHIFRFGTSFHIPNTGLTVGGGVSAQSGTSSLYNI
                     RQGGYGLIDGFVRYELGKHAKLSLIGTNLNGRTYFENNYNRTRGANNFYGEPRTVSMK
                     LDWQF"
     gene            complement(1312..3171)
                     /locus_tag="WJK66_001629"
     CDS             complement(1312..3171)
                     /locus_tag="WJK66_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ7579316.1"
                     /translation="MNQNHFSLKILTVMLLSAYGGSFADGVVPVSDGNTVSLDTVNVR
                     GSHALSGKTEKTRSYTIDRMSTATGMRIAGKDTPQSVSVITRSRLDDKAVHTLEEAMK
                     NTTGVNVVRDSGLQTRFLSRGFYIDQIGEDGITVNVAGRSGYTAKIDVSPSTDLAVYD
                     HIEVVRGATGLTQSNSEPGGTVNLIRKRPTASFKHTGELTADHRGSRRAVLDVSGSLN
                     KANTLRGRLVGAEEYKKSFKDRVWGRKHMVYGIAEADAGDSSVLTLGGMYQKSREVPD
                     FSGIILSCENQKTAPFSSTPACNRPLQLPRNTYLGEDWSRLSADKYNLFSGFKHVFDN
                     GWQLNAEVSYTKNESDAKVGQFFLKNEHAAGLSDEDAVGFLTEKNEVIPFEPKDKALE
                     KLKAYRDETAKEYRERKDDFVKNRFDNTAFEQYRSRRAAERKAGFDECMSAPFALDFI
                     CQGSWGDPGVDADKSEFVDKALAKEGIFNNAAQRFPNSLYDSSFNRKATANRRYSYMP
                     LRHTKDDRQWGIKLDLTGTYGLFGREHDFFVGYAYGDEKIRSEYLEIYERRHRVRPNT
                     GATHGVYAGSCQGSRTVICLLLWSGGIKNPIGRRTMKKATVPFMPKNAGMPRK"
     gene            3445..4224
                     /locus_tag="WJK66_001630"
     CDS             3445..4224
                     /locus_tag="WJK66_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ7579317.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYRHSFHFVPVTTRAANPSGLSGKRIPELLKNSSIEQALHTKFTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            complement(4435..5847)
                     /locus_tag="WJK66_001631"
     CDS             complement(4435..5847)
                     /locus_tag="WJK66_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ7579318.1"
                     /translation="MLFEFKENKMIVASRWPLLEKVFLRFGMPVAVVDLESTGGNLYE
                     DRVTEVALVKFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFAEIAGELF
                     SVLKGCVLVAHNSRFDYTFLKHEFHRAGIGFSSPALCSVQLSRCLYPQFYKHSLDSII
                     ERLGIVVEDRHRAMADVSALCDYLEYSLSEHGVEAWIRQCFRLMNPKPLPAALPERLR
                     EQLYGLPDGMGVLACFDGGGKVNYIGTFERVYSEVSALLDSGKAPFDWCNTEEVRFFP
                     ALGSLHAYKIKAELVGRYHSGCYVSAKNLLKTFTTVRFEKGSDGMLNAKTAALKNGVT
                     DNPPTGLFANKKAARRALSSWAETYGLCPASAGILPDGYAEDEPCPVYVSGRCDKACG
                     RSDEQVLAFAHKLPILDWGKMHEVEITETDPLTGEKVVLHGMGGALEMGDGLWYFDKD
                     LPDAFKAKFKTDRKNIKEIG"
     gene            5938..6014
                     /locus_tag="WJK66_001632"
     tRNA            5938..6014
                     /locus_tag="WJK66_001632"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5972..5974,aa:Arg,seq:acg)
BASE COUNT         1553 a         1725 c         1295 g         1512 t
ORIGIN      
        1 gccccgatag acctgcctcc ataaaaatgc cgtctgaaac ataccctgtt tcagacggca
       61 tccgcaaaac tacggtttcc aattaaaact gccaatccag tttcatgctg acagtgcgcg
      121 gctctccata gaagttgttt gcgccgcgcg tacggttgta gttgttctca aaataagtgc
      181 gtccgtttaa gttcgtaccg atgaggctca atttagcgtg tttgcccaat tcgtaacgga
      241 cgaaaccgtc tatcagcccg tagccgccct gcctgatgtt atacagactg cttgtgccgc
      301 tttgtgcgga cacgccgccg ccgacggtca gccccgtatt cggtatatgg aagctcgttc
      361 cgaaacggaa tatgtgcacg ggtgtgaaat tgctgaagtt gtacgggtct gcgcctgtgt
      421 ttttggcgag gcgttcggcg ttgacttcgg cggcgttttt gtagcggctc ttgttgtagg
      481 tgtaacccgc aaagactttc caatcttcgt tcaactcacc cgacaactcg aattccgcac
      541 ccctgctgac cactttgcct atcggtttgg caacggtttg gaacgacccc tgcttgccgc
      601 ctgctccggg aacatagcca aaatcgacga ccgtgcggtt tttctgttcg aggtaaaaca
      661 atgcgaacga agcattcagc cgtccttgca agaacgcgcc tttccagcct acctcatagt
      721 ttgtgccgac caaaggcggt aaaacggttt tggcactgac atcgacatta tcctgctgtt
      781 tgaagatttt ggtataactt ccgtaaatac tctgttgcgg tgtcaaatca taggtaatgc
      841 ctgcataggg cgtcaattta tgaccttgca tcttggccgt ataatggtcc tgatccgcct
      901 taatgctcga tgccgtctga aaatcgcttg ccggctgccc atagcggaca ggcatatctt
      961 tggtttgcga ggtttcatag cgcgtgtagt gcagcccgcc caaaagatgc agtcggccgg
     1021 ttacgttgaa acgcgtgccg gcagtcagcg aatgggtttt gttggtgttg aggtatttgg
     1081 cgtagttata cagcgcagga acatggtcgt cggccacttt gacggttttc caaaccggca
     1141 ccgtaccgga aaaaccggta aaggcaggcg tgccgtcggg attggtctcc tgaatcttat
     1201 tgcctttttc atccagttca tatacatcga catatactgg cgtccggctg ccgctgtatt
     1261 cgtcatagta atacacctgc ttgccttcgg catcgagctt gggctcggtt tttattttct
     1321 tggcattcct gcattcttcg gcataaacgg tacggttgcc tttttcatcg tacgcctgcc
     1381 aatcgggttc tttatgcccc ctgaccaaag gagaagacaa atcaccgtcc ggctcccctg
     1441 acaacttccc gcatacacgc cgtgcgttgc ccctgtattc ggacgtactc tgtggcggcg
     1501 ttcgtagatt tccagatatt cggaacgtat cttttcatcg ccgtaggcat agccgacaaa
     1561 gaaatcatgc tcccgcccga acagcccata tgtgccggtc aggtcaagtt taattcccca
     1621 ttggcggtcg tctttggtat gccgcaacgg catataactg tatcgtcggt tggcggtagc
     1681 cttccgatta aaggaagagt catacaggct gtttggaaaa cgttgtgccg cattattaaa
     1741 gatgccttcc ttcgcaaggg ctttatcgac aaattccgac ttgtcggcat caacacccgg
     1801 atccccccaa gaaccttgac agataaagtc cagcgcaaaa ggggcactca tacactcgtc
     1861 aaaaccggct ttgcgttctg cggcacggcg gctgcggtac tgctcgaaag cagtattatc
     1921 gaaacggttt ttaacaaaat cgtctttgcg ctcacggtat tccttggcgg tttcgtcacg
     1981 atatgctttc agtttctcca atgccttatc tttcggctcg aacgggatga cttcgttttt
     2041 ttcggtcaaa aagcctaccg catcctcatc tgacaaaccc gccgcatgtt cgtttttcag
     2101 aaaaaactgc cccaccttcg catcggattc attcttggta taagacactt cggcattgag
     2161 ctgccaaccg ttgtcaaaca catgtttgaa acctgagaaa aggttgtatt tgtcagcact
     2221 caaccgcgac caatcctccc ccaaataagt gttgcgcggc agttgcaaag gccggttgca
     2281 ggcaggcgtt gaactgaacg gggcagtttt ctgattttca caggacaaaa taatgcccga
     2341 aaaatcagga acctccctac tcttctgata catgccgccc aaagtaagca cactgctgtc
     2401 gcccgcatcg gcttcggcaa tgccgtaaac catatgtttc ctgccccaaa cgcggtcttt
     2461 aaacgatttt ttatactctt ccgcacccac caaccttccg cgtaaggtat tcgccttatt
     2521 caggctgcct gaaacatcca acactgcacg ccggctgccg cgatggtcgg cggtcagctc
     2581 tccggtatgt ttgaaagaag cggtaggtcg cttacggatc aaattgacgg ttccacccgg
     2641 ctctgaattg gattgggtca accccgttgc accccgtaca acttcaatat ggtcataaac
     2701 cgccaaatcg gtactcggag acacgtcgat tttcgccgta tatcccgaac ggcctgcaac
     2761 attgacggta ataccgtctt caccaatctg atcaatatag aaaccgcgtg acaaaaaccg
     2821 cgtctgcaag cctgaatcgc gcacaacgtt gacacccgtc gtgtttttca ttgcctcttc
     2881 aagcgtatgc accgccttat cgtcaaggcg gctgcgcgtg atgacgctga ccgactgcgg
     2941 cgtatccttg cccgcaatcc tcatacctgt ggcggtggac atccgatcta tcgtataaga
     3001 acgggtcttc tcggtcttgc ccgacagagc atgagagccg cgtacattga ccgtatccag
     3061 actgacggta ttgccgtctg aaacaggcac aacaccgtct gcaaaagaac caccgtaagc
     3121 cgataacagc ataacggtca gaattttaag tgaaaaatga ttttgattca tagagacctc
     3181 tgtaatatgc aagtgtgcaa atcgtccaaa ggccctcaca actgttttga ttttttatat
     3241 taattgaaaa aaagtaattc tcaattaaat ttatagatgg gattgtattc ccattttgac
     3301 aaaaaacaaa ctgctcctta ccgtttattt caaaaaacga taatattgta ttgaaaaata
     3361 tccgaattta aatacagatc gtcaatgcgg aaaaaaacac ccaaattggc tataatcccg
     3421 acaaacacac tcaaggacaa caacatggca gcctcgcccg aagcaaaatt cactgaagaa
     3481 aagattttgt gggtcaaaca ccacacgccg aaactcatca ctttcgccat cagccgtccc
     3541 gaatcctacc gttttaaagc cggacagttc tcccgactcg gtttctacga aggggaaggc
     3601 tttatttggc gtgcctactc catcgtttcc gcagaatatg cagacacgct cgaatatttt
     3661 gccgtactca tccaagacgg ccccatgtcg gcccgtttcg ccaaaatgca acagggcgac
     3721 accatcctgc tcgataaaaa tgccaccggc ttcctcctgc ccgaacgctt ccccgacggc
     3781 aaggatttgg tgatgctctg caccggctcc ggcatcgccc ccttcctttc cattctcgaa
     3841 caacctgaaa tccgacagcg tttcgatacc gtcaacctga tacattccgt atcttttccc
     3901 gaagaattga ttttcaacga ccggctcgcc gcattgtccg aacatcccct ggtaggcgaa
     3961 tacagacact cgttccattt cgtccctgtt accacccgtg ccgccaaccc ctcgggctta
     4021 agcggaaaac gcattccgga actcttaaaa aacagcagca tcgaacaggc gctgcatacc
     4081 aagttcaccc cggaatccac acggtttatg atttgcggca acccggaaat ggtcaaagat
     4141 actttccaaa cgctgcttga catgggttac gccatgcacc gcaaccgcat tcccggtcaa
     4201 atcatgatgg aaaacggctt ctaaaaacca ccctgcttgt ccgatgcctt cggatggacg
     4261 gtcaaaccga cacggcacga aaaccgcatc ggcaaaaatg ccgtctgaaa aaattcagac
     4321 ggcatcttcg gacacattac ctgcaaacgg caacacattc caacccaaac cgattaggca
     4381 atcaatacga acggctgttt acatacttac cggctttcat caaccggtat cgatttaacc
     4441 gatttcctta atatttttcc tgtccgtttt aaacttcgcc ttaaacgcat ccggtaaatc
     4501 tttatcgaaa taccaaagcc cgtcacccat ttccaatgcg ccgcccattc catgcagaac
     4561 aactttttcc cccgttaaag gatcagtttc ggtaatttct acctcatgca tttttcccca
     4621 atccaaaata ggcaacttgt gtgcaaacgc caaaacctgt tcgtcagaac ggccgcacgc
     4681 cttatcgcat cggcctgaaa catatacggg acaaggctca tcctctgcat aaccgtccgg
     4741 aaggataccg gctgaggcag gacacaatcc gtatgtttcc gcccacgacg acaaagcccg
     4801 tctcgccgcc tttttattgg caaataatcc ggtaggcgga ttatccgtca caccgttttt
     4861 caaagccgct gttttcgcat tcaacatgcc gtctgaacct ttttcaaacc tgacggtcgt
     4921 aaatgtttta agcagatttt tggcagatac ataacaaccc gaatgataac gtccgaccaa
     4981 ttccgcttta atcttatatg catgcaggct gcccaatgca ggaaaaaaac ggacttcctc
     5041 cgtattgcac caatcaaacg gggcttttcc ggaatccaat aaagccgaaa cctcgctata
     5101 tacccgttca aacgtaccga tgtaatttac tttccctccg ccgtcgaaac aagccagcac
     5161 ccccataccg tcaggcaaac cgtacaactg ttccctcaac cgttcgggca gcgcggcagg
     5221 cagcggtttc ggattcatca aacggaaaca ctgcctgatc catgcctcaa ccccatgttc
     5281 cgacaaactg tattccaaat aatcacacaa tgccgacaca tccgccatcg cacgatgcct
     5341 gtcttctaca acaatcccca acctttcgat gatactgtcc aggctgtgct tgtaaaattg
     5401 cggatacaga caccgggaca gttgcacact gcacaaagca ggcgatgaaa acccgatgcc
     5461 cgcacgatga aactcatgct ttaaaaacgt atagtcgaaa cggctgttat gtgcaaccag
     5521 cacacaaccc ttcaataccg aaaacaactc gccggcaatc tctgcaaaaa caggcgcatc
     5581 ggcaaccatg ccgtctgaaa tccccgtcag ccccgccaca aactgcggaa tcggtttttg
     5641 aggattaacc aaccactcat gcctcaccac ccttccctgc tcaaacttga ccaaagccac
     5701 ttcggttacc ctgtcttcat acagattgcc gcccgtcgat tccaaatcga ccacggcaac
     5761 aggcattcca aaccgtaaaa ataccttttc cagcaagggc cagcgggaag caacaatcat
     5821 tttattctct ttaaattcaa acaacaaacc gatattttac acttttaagg catttcatcc
     5881 aacaaaacaa ttgacagaat ccgatgatta ccctaaaatt caaatctttc ttgcagtgca
     5941 cccgtagctc agttggatag agtatctggc tacgaaccag agggtcgggc gttcgaatcg
     6001 ctccgggtgc gccagtaaga aaatacaata tgcgcccatc gtctagcggt taggacatcg
     6061 ccctttcacg gcggtaaccg gggtt
//
LOCUS       DAWKOG010000123         6035 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.123, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000123 DAWKOG010000000
VERSION     DAWKOG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.123"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(223..1560)
                     /gene="ribB"
                     /locus_tag="WJK66_001633"
     CDS             complement(223..1560)
                     /gene="ribB"
                     /locus_tag="WJK66_001633"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEZ7579319.1"
                     /translation="MTDTAGLRRHNLRQWIEKYYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPPEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAVNFMIKHARGLVCLPMAGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPAAVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPAADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERIQQAESGVVILLHRAEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILVGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            complement(1776..2785)
                     /locus_tag="WJK66_001634"
                     /pseudo
     CDS             complement(1776..2785)
                     /locus_tag="WJK66_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273336.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(2941..3534)
                     /gene="ribA"
                     /locus_tag="WJK66_001635"
     CDS             complement(2941..3534)
                     /gene="ribA"
                     /locus_tag="WJK66_001635"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEZ7579320.1"
                     /translation="MSELLNYVASCRLPTEWGVFTMHGFEEANRQEHVALTVGNFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(3527..4201)
                     /locus_tag="WJK66_001636"
     CDS             complement(3527..4201)
                     /locus_tag="WJK66_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579321.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMDMGTASIDRSV
                     LWLEHEDWRLADYLEQNSGNEEGLKRLFMALDSVFSRCKGVRAAAVYALMPSENAAFR
                     LVCLSQQGEGLENIWNLDENVADVSLACRCAQSGWMNVASDVRRWLDLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECAGTAAQVEWVALALALSEPLKLLLGITAA
                     EGDENV"
     gene            4355..4439
                     /locus_tag="WJK66_001637"
     tRNA            4355..4439
                     /locus_tag="WJK66_001637"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4389..4391,aa:Leu,seq:tag)
     gene            4960..5994
                     /gene="purM"
                     /locus_tag="WJK66_001638"
     CDS             4960..5994
                     /gene="purM"
                     /locus_tag="WJK66_001638"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEZ7579322.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYKNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
BASE COUNT         1459 a         1491 c         1589 g         1496 t
ORIGIN      
        1 gccgttcttt ccctgaaaga gagaatccaa aaaaccaaag ccacaggaat ttatcggaaa
       61 tgacaaaaac ccgacgaacc ggattcccgc ctgcgcggga atgacgaatt agaagttacc
      121 cgaaatttgc aaaaacaaaa tccaacccaa cagaccgggt tttcgtttgc acggaaataa
      181 tgcaataaat aaagcaaata taaagtattt gaatttacta tattattttt ccgcttcttc
      241 aaagccgacg acttccaaac cgaagccggt caggccggtg aaagatgagg gctgcccgag
      301 gacgcgcagt tttttgacgt tgaggccgac gaggatttgt gcgccgatgc cgtagctttt
      361 gctgtcccat ttgtaggctt ggtttgcacc tttgggcagg gtgcggtcga gcagggatgc
      421 gccgtcttcg gcgcggtgta agaggatgac gacgccgctt tcggcttgtt ggatgcgctc
      481 aagggctttg ggcagcgacc atgaatggcg cggattggct tggatgaagt ccatcacgct
      541 gaagggttcg tggacgcgga cgagggtttc ggtgtcggcg gcgggcgtgc ctttgacgag
      601 ggcgaggtgg gtttcgccgg agagtttgtc gacgtaaacg tgttgttgga actcgcccca
      661 cggggtttgt acaggcgcat tgcccatgtc ttcaagcagg ctttcggtac ggctgcggta
      721 ttcgatgagg tcggcaatcg tgccgatttt gagcttgtgt tcttcggcga atttcatcag
      781 ttcgggcata cgcgccatcg tgccgtcgtc gttgatgatt tcgcagatga cggcggcggg
      841 aatcaatccg ttcatttgcg ctaaatcgac accggcttcg gtgtgtccgg cgcggacgag
      901 tacgccgcct ttttgcgcgc gaagcgggaa aatatggccg ggttggacga tgtcttcggg
      961 tttggcggtc ggggaaacgg cggtttgaat ggtcagggcg cggtcggcgg cagaaatgcc
     1021 ggtggtaatg ccgtgtgcgg cttcgatgga gacagtaaag ttggtgccgt attgcgcgcc
     1081 gtttttctgg gtcatcatcg gcagtccgag tttttcgacc atttcgcccg ccatcggcag
     1141 gcagaccagg ccgcgcgcgt gtttaatcat aaagttgacg gcttcgggcg tgacgaattg
     1201 cgccgccatc agcaggtcgc cttcgttttc gcggtcttcg gcatcggtga tgatgaccat
     1261 tttgccggct ttgatgtcgg ccaggatttc ggggatgggg gagatgtggg acatggtgtg
     1321 ttttcctgtg tgttcgggcg ggcgggcggt gtgttcggta tcgagccaaa aggcgggcat
     1381 ttttgccgcc tgttcgattt tacgggcttt tttctcgccg aaggatttgt ttttcaaaag
     1441 ggcggagagt tcgcctgtgt tgagggcaac ggcttcagca aaacgagttt gcaaaccgcc
     1501 gtagtatttt tctatccact gccgcaggtt gtggcggcgc agaccggctg tgtcggtcat
     1561 cgtttcatcc tttgtagaaa tcggatgtag cggattgtaa tcgcgtattt accaaaaagc
     1621 aaatctgttt ttttcgccaa accgaattat ttgctttttg gtaaacagat gccgtctgaa
     1681 gccggattcg tggcttcaga cggcattgcc gcaatccccg ccggcggggc ggctgtttct
     1741 tcccgcccgc ttccgattcg gggtttgccg gaagcttatt ccgaaaaaaa tttttgataa
     1801 acggccttga ttgccgggta cagcataggc ggtacgagca ggcgcaatat cggcaatcct
     1861 atgatgaatg cggtcttccc gataaaaacc ggacggattg ccttcgcgcc gaatatcttt
     1921 ctgcattttt ctttaaatga aaaatatttc cggtagatga ggataatgtt tttggaaaaa
     1981 taaagcctta ccaatataga cctcagatag ccgatattgt cgaagacaaa tttttgcaaa
     2041 ccgccgtaga tgggggaatt tttgttttcg actaaaaaat ccacaccccg gtcgagatgc
     2101 gtcagcacat cgctgaaatt tttgtatttg atgttgtggc tgatagagcc gccgagaacg
     2161 cggtactggt aaaaagggtt atcgtaaaag gcaaaagtct tgataaaacg cgccaattgc
     2221 aaactgtacg ggaaatcctc gtgaatgtat ccttttggga aaacagattg tttttgatga
     2281 ttatttcccg cctgacaatc tttgtccacg cgttggcgat atagtaccgc ccctccacca
     2341 gcgtttcaaa atggcggaca aaatcattat ccgaaaagtc cgcccctttg gggatgtcgc
     2401 ggtaattgag ggacgaggga tgcaggatca aatcaacctt tttgtctgca agttgttgta
     2461 aatcaaagag aatccacccc gcgttttttg aacggttggt atcggcccaa tagtcgtcgc
     2521 tgtccaaaaa gattaggtaa tcgccttttg ccgcccggat accggcgttg cgggcatccg
     2581 acagcccgcc gttttcttga tgaatcactt ttatatgcgg atatttgcct gcatattcgt
     2641 cgcaaatctt cccgcagccg tccggcgaac cgtcatcgac caaaatcatt tcataatcgg
     2701 caaaattttc ggcaagcacg gaatccacgc agcagcggag atatttttcc acattgtaaa
     2761 tagggacgat gatggagaaa atcataaata tcaatccatt atattaagat gtttgcgcgt
     2821 atgcctcaaa cccgcacccg caatgcgttt gcatccacta taaaacttat tgctttatca
     2881 aggtatggaa acctgtttcc cgaaaggcgg cgcaggatgc ccgttccctg caactttgcc
     2941 ttattccgac atcagatgtc ccagcttgtc tgctttggtt tggaggtagc gtttgttttc
     3001 aaggttttcc ccgacgtgca gggggatgcg ttcgaccacg ttaatccccg catctttcag
     3061 ggtttggatt ttttcggggt tgttggtcag cagtttgacc gagcggatgc ccagatattc
     3121 gtagatagat tgcgccaaac ggaaatcgcg ggcatcgacg ggcagcccga gtgccaaatt
     3181 ggcttcaacc gtatccatac cttggtcttg cagatgatag gcgcggattt tgttaatcag
     3241 cccgatgccg cgtccttcct gacgcagata gacgatgatg ccgcgcccct ctgtctgtac
     3301 cgccctcatg gccgcttcaa gttgaggtcc gcagtcgcat tttctcgaga acagcgcatc
     3361 gcccgtcagg cattcggagt ggatgcgcgt cagaaccgga ttgccgtctg aaaaattacc
     3421 gacggtcagc gcgacgtgtt cctgcctgtt tgcctcttca aagccgtgca tcgtaaatac
     3481 gccccattcg gtcggcaggc ggcaggaagc aacatagttt aataattcag acattttcat
     3541 ctccttctgc ggcagtgatg cccaacagca gtttcaaagg ttcggacaaa gccaaggcaa
     3601 gagccaccca ttccacctgt gctgccgtac ccgcacactc tgcgcattca aattccacat
     3661 gaaccacgcc caacacaccg ccactttccg tgcagaccgg aatggaaatt tgcgccgccg
     3721 aagcatgatt gcgttctccc gaaagctctc ccaaatccaa ccaacggcgt acatccgagg
     3781 caacattcat ccagccgctt tgcgcgcaac ggcaggcaag tgaaacatcg gcaacatttt
     3841 catctagatt ccatatgttt tccaaacctt ctccctgttg ggacaggcaa accagccgaa
     3901 aggcggcatt ttcagacggc atcagggcat aaacggctgc ggcgcgcacg cctttacagc
     3961 gcgaaaatac cgaatccaga gccatgaaaa gccgtttcag accttcctca ttcccgctgt
     4021 tttgctccaa ataatcggca agcctccaat cctcatgctc aagccacaaa accgaacggt
     4081 ctatcgaagc cgtccccata tccattaccg tctgcgccgt cagatacgcc gcccgaacct
     4141 cgtcaagcgg cagcttcaaa ccctgagtca gcagaaaatc cttaatcaaa gcagcaggca
     4201 tacccaaatt ccgtcaataa aaacaaaaaa ccgcccgatt ccgatgtcag gcggacgaaa
     4261 aacagatttt accgccccaa accccaagcc atcaacagcc ccgccgaaaa ccttgcacaa
     4321 aaaaacaaaa tctgtataat cgtccaattc ccatgcggac gtggcgaaat tggtagacgc
     4381 accagattta ggttctggcg ccgagaggtg tgagagttcg agtctctccg tccgcaccaa
     4441 taatgataaa taaattatat agttaatgct tatcgggaca aattagtgcc acaaaacata
     4501 agtcgtctgc ttttcagcgg gcggcttttt tatctgccta tatgtttcaa cacgcagaac
     4561 ggtacataaa gtgccgccat ttgttccgca ccgaatcgga aacgggtgct tccgcccgca
     4621 atcagattca ctgcccctaa atgccggggt ttcaaccgga aagaagaaaa tattgatttt
     4681 gatgatggca tcaaaaaacc ccttccgccc gtctgccaaa aggaatggtt aagaaaagca
     4741 atgcgtcttt tgatggaacg cgatatataa ggaattgagg ggaagggcag gcgggtttct
     4801 gcccgttaat cctgcggacg gtcccgccaa acccgacacg cgcgggcata tggcggattg
     4861 tttacaatat tgtctaaaaa tctgaacctg caaacgtgaa ataaggtaga atacgccccg
     4921 ttattttacc gtccaatctc catttaccaa ggaaaaacga tgagtacttc attgagttac
     4981 cgcgatgcag gtgtcgatat cgacgcaggc gaccaactgg tcgaaaacat caaaccgttt
     5041 gccaaacgca cgatgcgccc ggaagtattg ggggatttgg gcggcttcgg cgcgttggtc
     5101 gaaatcggca agaaatataa aaatcccgta ttggtcagcg gtacggacgg cgtgggtacc
     5161 aagctcaaac ttgcctttga ttgggataaa cacgatacgg tgggcatcga ccttgttgca
     5221 atgagtgtca acgatatttt ggttcaagga gcagaacctc tgtttttctt ggattatttc
     5281 gcctgcggca aattggatgt tccgcgcgcg accgatgtga tcaaaggcat tgcacaaggt
     5341 tgcgaagaat ccggctgcgc cctgattggc ggggaaactg ccgaaatgcc gggtatgtat
     5401 cccgtcggag aatacgattt ggcgggtttt gctgtcggcg tggtggaaaa agagaatgtc
     5461 attaccggcc gcagcatcgg cgcgggcgat gtggtattgg gtttggcttc caacggcgca
     5521 cattcaaacg gctattccct tatccgtaaa atcatcgaac gcgacaatcc cgatttggat
     5581 gccgagtttg ataatggcaa aaccttgcgt gaggctgtta ttgcgccgac ccgtctgtat
     5641 gtgaaaccga ttcttgccgc tttggaaaaa tttaccatta aaggtatggc acacattacc
     5701 ggcggcggca ttaccgaaaa cgtgccgcgc gtgttgcctg aaaacacggt tgcacaaatc
     5761 gatgctaaat cgtgggaatt gcccaagctc ttccaatggc ttcaaaaggc gggcaatgtg
     5821 gaaacccaag aaatgtaccg gacctttaac tgcggcatcg gcatggtcgt tattgttgct
     5881 gccgaagatg ccgatgcggt tcagggtctc ttgggtgaac aaggtgaaac agtttaccgt
     5941 ttaggtttga tccgtgagcg tcagggagac gagcatcaaa cccaggttgc ctgattgctt
     6001 ctatagtgaa atgccgtctc aaacatgggt ttgga
//
LOCUS       DAWKOG010000124         6023 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.124, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000124 DAWKOG010000000
VERSION     DAWKOG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.124"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(57..1460)
                     /gene="mtrE"
                     /locus_tag="WJK66_001639"
     CDS             complement(57..1460)
                     /gene="mtrE"
                     /locus_tag="WJK66_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HEZ7579323.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANDSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPDDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGSVDTHSAELGGLFKSGTGVWL
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYEAAVQSAFQDVANALTAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYSAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(1515..4718)
                     /gene="mtrD"
                     /locus_tag="WJK66_001640"
     CDS             complement(1515..4718)
                     /gene="mtrD"
                     /locus_tag="WJK66_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HEZ7579324.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LHATYPGASAQVMEDSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSFEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            complement(4730..5968)
                     /gene="mtrC"
                     /locus_tag="WJK66_001641"
     CDS             complement(4730..5968)
                     /gene="mtrC"
                     /locus_tag="WJK66_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HEZ7579325.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAEQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPTVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFIVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIAGITGAKKVTPKEWAPSENQA
                     AAPQADVQTASEAKTASEAE"
BASE COUNT         1351 a         1699 c         1624 g         1349 t
ORIGIN      
        1 aaagaaatgc cgtctgaaac cttatcgttt cagacggcat tttattgccc gaccggttat
       61 ttgtcggttt gggtatcccg tttcaatccg ccgccgagtg ccttgtacaa atcggcaagg
      121 ttttcggcgc gggtcagttg tgccgacaaa gccgcacctt ccgccgaata gctgctgcgt
      181 tccgcatcga gcaagtcgag cgcgccggat acgccgtgtt tgtaacgcag accgaccaaa
      241 cgcaacgctt ctttagaggc gcggctttgt ttgcttaaag cgtcataggc tttatccaac
      301 tgctcgcgcg cggtcaatgc gtttgccacg tcttgaaatg cggattggac ggcggcttca
      361 taggcaacga tttgtgcctg ttggcgcagc ttggctacat cgaggttcgc cttgttcgta
      421 ccccaggtaa aaatcggcag ggtaatggaa ggtgcgaaca accaaacgcc ggtgccgctt
      481 ttgaacagcc cgcccaattc ggcagaatgc gtatcgacgc ttccggtcag gcggatggat
      541 gggaaaaagg cggcgcgtgc cgcaccgata ttggcgtttg cctgtttgag cgcgtgttcg
      601 gcagcacgga tatcgggacg gtcgagcaat acttcggaac tcaaaccggc cggcagcttc
      661 tcaacaaaaa actgcttgtc caacggcaaa ccggcgggca ggtcgtcggg tatcggttgg
      721 ttaatcaggg ttgccaaggc attgcgcgcc tgttcgcggc tgcgcgcggc atgggcataa
      781 tcggctttgg cagattcgat tagggcttcc tgctgacgta gggcgacggc ggaaatcacg
      841 cctgccttgt aacgtaattc ggacagcttg taggtttcct cgcgcgtttt caaaacacgt
      901 tgcgccaaag acatcgcttc ttcggcataa cgttcgttga aataggcttt ggcaacggtg
      961 gcaatcaggc tcaaatgtgc cgcatcgcgg ttggcggtgc tggcgaaata gccttgcagt
     1021 gccgcctcgc tgctgctgcg tacacgcccg aacagatcga gttcgtaaga tgccgcaccc
     1081 agtccgactt tgtagctgct gcttacattg ccgccgctca agctgccttg gcgcgagtcg
     1141 ttcgcattgg cggcaagcgt gggcaggagg ttgttgcgct caatcatgta ttgtttgcgg
     1201 tagatttcgc tgttcaatac ggcggtacgc aaactggtat tgcgctcgag tgcgatgtcg
     1261 atcagctttt gcaggcgcgg gtcggcaaaa tagtcgtgcc aacccaaatc gacggcgcgg
     1321 atgccgctgt cggcggtatc gtttttgaac gtttcggcaa cttcgacttt gggctgctcg
     1381 tattggggaa tcatggtgca ggcggataat gcgaaggctg ctgcaacaga agtcaaggtg
     1441 gttttcaatg tagtatccat aaaaaagtcc tgatgccgtc tgaaaacccg tgggcgttca
     1501 gacggcatgg ttgcttaatg ttggctgtcg tccgaaccgg tgatgcccgc ttcggcagcg
     1561 tgttttactg ccatttcgtg ttcgtgcgcg gtttctttga agaatttgcg caccaccaca
     1621 tagaaaagcg gaacaaggaa caccgacaag agcgtgccga tgagcatccc ccagaatacg
     1681 gttgtaccaa tggcgcgctg gctggcagaa cttgcaccgc cggcaatata caggggaacc
     1741 acgcccaaaa taaaggcaaa cgaggtcatg ataatcggac ggaaacgcag gcgggctgct
     1801 tccaaagcgg cttcaaccgc gcttttccct tgcgcttgaa ggtctttggc aaattcgata
     1861 atcaaaatcg catttttcgc actcaaaccc atcacggtaa cgaaaccgac ttgaaagtag
     1921 atgtcgttgg cgaacgaggg aacgctgccc aacagtcctt caaacaggtt gcgcccggtt
     1981 acgcccgcag ccgcaccgat caaacccaac ggaatcacaa ggatgaccgc cagcggaatc
     2041 gaccagcttt cataaagcgc ggcaagtacc aaaaatacgg ctgcaaccgc caaaccgtac
     2101 aaaatcaggg tttgcgagcc gccttttgcc tcttcgcgcg actgtccgcc ccattcgaag
     2161 ctgtaaccgc cgcccaattc gtcaaccatt ttttgaaccg ccgccatagc ctgcccggtg
     2221 gaaacgccgg ttgcaggcga agcggacagc ttcatcgaag gataaccgtt gaagcgtacg
     2281 ctctgttccg taccgttttc ccaagaaaca gtagcaatgg tggaaagcgg tacggcgacg
     2341 ccggatttgt tcggcacggt caggttcaaa atatcggcag gctgcatacg ggcatcctcg
     2401 tcggcctgca ccatcacgcg ttgcagacgg ccttggttcg ggaagtcgct gacataagac
     2461 gaactcagcg cgcttgccaa tgcggtgcgg atgtcggcaa acgaaatgcc ttgcgccgcc
     2521 gcggcggcac ggttgatgtc gattttcaac tgcggcgagt cttccaaacc gccagcacgg
     2581 acggtgctgg ggtcaaacaa accgctggca cgcattttct gaatcaactc gttgcgcttc
     2641 gccagcaatg cggtatggcc ggtattattg cggtcttgca ggttgatgct caggcccgaa
     2701 ccattgccca actccagaat cggaggcggg acgacggcga tgccaaaacc gtctttaagc
     2761 gtccccatca tcatacccgt cagcttgccg gcaatcgcaa cggcatcgct gccgggcgcg
     2821 gtacgctcgt tccaatcttt caatatggca aaacccatcg ccatattctg accgctgccc
     2881 gaaaagctga agccggaaac ggtaatgatg ttttctattt caggaatgct tttcgccagt
     2941 tgggtaactt gcgccaaagt cgcattggtg cgctcttggg tcgctcctgc aggcagttgc
     3001 acgctgacca tgacgaagcc ttggtcttcg gtcggcagga atgaagtcgg caggcgcata
     3061 aacaggaaca cgcccacaac cgccaagccg atatagacaa ccatcatgcg gaaagtctta
     3121 cgcagcactt tggcaacccg gccttcgtaa ccgtgcgtcc aactgttgaa tttcttgtta
     3181 aaccagccga agaaaccttt tttctcttcg tgatgccctt tcgggattgt cttcaacatt
     3241 gtggcacaca aagcaggggt aagggtcagc gcaaggaagg cggagaatgc gattgatgac
     3301 gccatcgtca gggcaaactg tttgtaaata ttgcccgtcg ccccgctgaa catcgccaac
     3361 ggtacgaaca cggaaatcag aacggcggta ataccgatga ccgcgcccga aatctgaccc
     3421 atcgcttttt tggtcgcttc tttgggcggc aagccttcac ccgccataat gcgctcgacg
     3481 ttttcaacca ccacaatcgc gtcatcgacc acgatgccga tgaccaaaac catcgcaaac
     3541 atggtcagta cgttaatcga catgcccata taagagatga aggcgaaacc gcccaacagc
     3601 gaaatcggta cgacgatggt cggaatcagc gtataacgga tgttttgcag gaagagatac
     3661 attacgacaa acaccagcac catcgcttcg attaaagtat gaatcacttt ttcaatcgaa
     3721 atttcgacga atttggaagt atcgtaaggg gttttccagc tcataccctg aggaaagtat
     3781 ttttccaacg tcgccatgcg ttctttaacc gcctttgccg tcgccatcgc attgccgctg
     3841 ttggacagca tcaccgccat accggtggta tttacaccgt tcagacgggt tgaggaagaa
     3901 tagtcttcca tacccagtcc gacccttgcc acatccttca ggtaaacatt agaaccgtcg
     3961 gtattggcgc ggaggatgac gttgccgaat tcttctgccg tacccaactg cccttgcgcc
     4021 gttacggtag ccgtaaccgt ctgtccgcga acggcgggaa gcgaaccgat agaacccgct
     4081 gaaatctgga cgttctgggc ggacagcgca ctgccgatat cggcaaacga caaattgtag
     4141 ttttgcagtt tcttaggatc aacccaaatc cgcatcgcgc gttgcgcgcc gaacaggcgt
     4201 acctgcccca cgccttcgat acgctgcaac tcgggaacga tattacgctg cgcgtagtcg
     4261 ttcatctctt cggttgactg cacatccgac gaaagcatca caatcatcag gaaattggaa
     4321 cgcgccttgg atacggttac gccgtattgc tggacggttg ccggcagcgt gctcaatact
     4381 tcggaaagct tgttctgcac ttccacctgc gccagattct cgtcggtatc gggcgtaaag
     4441 gtcaggctca cgctgccgct gccgctcgaa tcggcggaag tggacatata atccaaacct
     4501 tccacgccgt tcatattccg ctcgatcacg gaaagcacgc tgtcttccat tacctgcgcg
     4561 gacgcacccg gataagtggc gtgcaaagta atggtcgggg cggcgacgga cggatattgc
     4621 gaaacaggca ggcttttgat gccgaaaata cccgccgcaa taatgaaaat cgaaataacc
     4681 cacgcaaaaa tagggcggtc gataaaaaat ttagccatcg atgccttcct tattccgctt
     4741 cagaagcagt tttggcttca gatgccgtct gaacgtcagc ttgaggcgcg gcggcttggt
     4801 tttcagacgg cgcccattct ttgggcgtta cctttttcgc acccgttata ccggcgatac
     4861 tgatgccttc cacaaccacc ttgtccccgt ccttcagacc cgacgtaaca atccaattcg
     4921 taccctgctg ttgcgcgacc gttacctcgc ggggttccat accgccttgg gcattcacaa
     4981 tcatcacggt atctttcgca ccgcgcgtta ccgcctgctg cggcacgatg aatgcgttat
     5041 ccaccgccac ttggtccatc agcacgcgca catacaggcc gggcatcagg atattctgat
     5101 cgttcggtac ggcggcgcgc agggtaatct gaccggtcga ttcgttgacg gtcggatcgg
     5161 caaacagcag acggcctttt tcagggtaaa ctgtgccgtc gtcaaatttg atgccgaccg
     5221 caatcacacc atccgccgcc agcagtttgc cttcggctat ctgacggcgc aatttcatca
     5281 cttcggatgc agactgggta acgttcacat acatcggatt ggtttggcgg atggttgcca
     5341 gcacggtcgt atcgcccgca ttcaacagcg taccttcgga aactttggac tgaccgataa
     5401 agccggaaat cggcgcggta atgcgcgaac ggttcagatt gatgccggca gatttgatcg
     5461 ccgcctgtgc tgctttgaca cctgcctcgg cagaacgttt cgccgttacc gcagcatcgt
     5521 attcctgccg gctgacggct tcggcggcaa ccaaaggctt gtatcgcgcc aaatccgcat
     5581 ccgctttggc aagcgttgcc tgagccgttg ccagttgcgc gcgcgcgctt tccagacctg
     5641 cttcataagt ggaactgtcg atctgataca gcggctgttc ggcacggaca taactgcctt
     5701 cttggaacag gcgtttttgg atgatgccgc cgacttgggc gcggacatcg gcggtacgca
     5761 gcgattccaa acgccccggc aactcgacgg tcaacgcgac ggtttgcgga tggacggtta
     5821 cgacaccgac gacgggcgcc ggtgcttccc gaccagcagg ctgcccgccc tgcgccgcgt
     5881 ctccgccttt accgcaagac gacagtacca atgcaacggc ggcagccaac gcggccgcac
     5941 gcatcgcctt aaaagcataa aaagccatta tttatcctat ctgtctggtt tgatgtaaag
     6001 ggttttgcca atcaacaggc att
//
LOCUS       DAWKOG010000125         6019 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.125, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000125 DAWKOG010000000
VERSION     DAWKOG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.125"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(53..361)
                     /locus_tag="WJK66_001642"
     CDS             complement(53..361)
                     /locus_tag="WJK66_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579326.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHDYGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKELLADLDKELC
                     "
     gene            complement(345..647)
                     /locus_tag="WJK66_001643"
     CDS             complement(345..647)
                     /locus_tag="WJK66_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEZ7579327.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(721..3084)
                     /locus_tag="WJK66_001644"
     CDS             complement(721..3084)
                     /locus_tag="WJK66_001644"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEZ7579328.1"
                     /translation="MQFSYSWLKTQADTELSADKLEHLLTMSGLEVEEAETAAPAFAG
                     VVVAEVKSVEKHPDADRLNVTRVDAGTGELVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVPSNGMLCSTNELGLPDDGVNGLHILPEDAPVGINIREYLDLDDTLFT
                     LKITPNRADCLSVKGIAREVSALTGCAFRQPEIHTAPITGSRKQPVQINAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISVLVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGALSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGELPEAKQVGLRLDRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRSRFAVYNEMAARGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIARVFGKGSDGRFVQNERIGGLWYGAAMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGYPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYRGM
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(3120..3638)
                     /locus_tag="WJK66_001645"
     CDS             complement(3120..3638)
                     /locus_tag="WJK66_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579329.1"
                     /translation="MSEIINLTESGITFNLVKNNLFYIEPYIRKRRQEGIKTVEFIYL
                     QEDNLLFVEAKQSIPNQERSPERFDEYISEIYQKWCNALNVEILGILGREDIKETIMP
                     SAFSNLQWGSIEIKLLLVIPDVPLNYLGQLNELIKQEFNKKDTLRLISLWNISVEVIN
                     RDLAIQKGLASS"
     gene            complement(3645..4709)
                     /locus_tag="WJK66_001646"
     CDS             complement(3645..4709)
                     /locus_tag="WJK66_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ7579330.1"
                     /translation="MIKSFWLENFGPISLLKADNLGKINLIIAENSKGKTFILKALYS
                     VLKSHEEAHKGKNIRDFSEELRDKLYWTFQVEEIGDLVTRGKENPNERPLKLSMTLED
                     SSSVLFSFGRTTKKLIKPELYELSPRINANSIFLPPKEVLSLFDVIKKSEEEKRFGFD
                     ATYIDLVKALDIKPTKGRNYPEAAEARKDLEALFGGYVSYNDKKKAWVYEKDRQTFSI
                     NSTAEGVKKIGILDTLLGNRFLSPGSILFIDEPEANLHPKAINQFLEILFSLSKSKLG
                     IQIFMSTHSYFVIKKLCLLARKHSESIPVFMPGKEKEQTTWVQENLQNSMPDNEIIGE
                     SIRLFREEIGVLSPETAWIG"
     gene            complement(4886..5878)
                     /gene="pheS"
                     /locus_tag="WJK66_001647"
     CDS             complement(4886..5878)
                     /gene="pheS"
                     /locus_tag="WJK66_001647"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEZ7579331.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKREALNEVKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLRNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK"
BASE COUNT         1541 a         1543 c         1253 g         1682 t
ORIGIN      
        1 attttttagc gtgtaaaaca aaaccggctg catacctgca accggcctca aatcagcaca
       61 attccttatc caaatccgcc aacagctctt tcagaccgtc tccgatttcc tgaagcgtaa
      121 ccggacggtt gccgtccgaa cccgactccg caccttctgc atacggtgca aacgtgctct
      181 gcaatttcaa cagtttcacc acatccaaac gggtgtagcg ttcgccgccg taaccgacaa
      241 ccacaccgta atcatgctgc cattgcgcaa aaccataggg gctgatttgc aacagtccgc
      301 acaactcgtc cagcgtgaaa tagcgttttg cgggaacctt caaatcaacg ttgtttgtca
      361 tagtagtgtt ccaccatgct tttgagtttc tggctggcat ggaaagttac cacgcggcgg
      421 gcggtaatcg gcacttcctc gccggtttta ggattacgac ccgggcgttg cggcttgtcg
      481 cgcaactgga aatttccgaa accggaaatt ttgatttctt cgccgcttgc caaagtgctg
      541 cggatttctt caaaaaagag ttcgacgatt tctttggcat cgtttttggt gacgttgctg
      601 actttgtcta ccaaaatatc ggccagttct gctttagtga gagtcatatg attaccttct
      661 gttgtaacag ttgtggatta ttaccaattt ttatttaaaa ttcaagcgaa tatttatttt
      721 ttaactgcga agccgcgccc ctgccgccgt tgccgcgcca atcagttttc cgataagcgg
      781 ctcgaccgcc tcatccgtca gcgtgttttc catatcctgc aaaatcactt tgaccgccac
      841 gctcttcatc ccttcgggca gtcccatgcc gcgatacacg tcaaacacgc tgatttcctg
      901 taccaacttg tttgccgcgc ctttcaagac aagcagcaaa tcatcatggc tcatagcttc
      961 cggcatcaca aacgccaaat cgcggcgcac cggctggaat ttcgatacgg cccgatagcg
     1021 cgttttcccg cattccaaca cggccgccat atcgatttca aataccagcg gcgcttgcgg
     1081 caggtcgtat ttttgcagcc atttcggatg cagttcgccg acaaagccga tgactttgcc
     1141 gtctgaaacg atattggcgg cgcgtccggg atgcagggcg ggatatccgg ttttaacgaa
     1201 ctcgaccgct ttgtttttca acagattttc cacatctgcc ttgatgtcgt aaaaatccgc
     1261 attgcgcgtt ttcccgcccc attgttcggg cattgccgcg ccgtaccaca agccgccgat
     1321 gcgttcgttt tggacaaacc ggccgtctga acctttgccg aacacgcggg cgatttcaaa
     1381 cacgcgcacg cggttttgtt tgcggttcag gttgttttgc aaaatttcca ccaagccgcc
     1441 gatgagtgtg gaacgcatca cggcatattg cgccgccagc gggttttgca ggcggatggg
     1501 gtcggcgttg gcggcaaaat cttgttccca ctgctcgtca acgaaggcat agctgaccac
     1561 ttcgcggtag ccacgcgccg ccatttcgtt gtaaacggca aaacgcgagc gacgtgtttc
     1621 gggcagttcc agcattttca gacggcctga cgtgtaatcg tcggggatgt tttcatagcc
     1681 gtaaacgcgt ccgatttctt caatcaaatc agcctcaatt tcgatgtcaa aacggaagct
     1741 cggcgcggta acgcggaagc cttccgccgt tttctcgggc tgcagaccca agtgttgcaa
     1801 aatggtttcc acctgttcgg caggaatgtc cacgcccaac acggttttca gacggtccaa
     1861 acgcaatcca acctgcttcg cttcaggcaa ttcgccttgc gcttccacca tctcgcctgc
     1921 cgcaccaccg caaatctgca acaccaattc ggtagcacgt tcaatggcat ccgcctgcaa
     1981 acggtaatcc acgccgcgct cgaagcggaa cgacgaatcc gaaccgaaac cgtattggcg
     2041 cgatttgccg gcgatgattt cgggcgcaaa ccaagccgct tccagcacga tattttgcgt
     2101 gccgtctgaa accgcgctcg ccgcgccgcc cattaagccc gccaaactca acgcgccttt
     2161 ttcgtctgcc acgaccagcg tattttcaga cagggaaacg gttttctcgt tcaggcattc
     2221 cagcgtttcc ccttcgcgcg cgcggcggat gtgcaggctg ccggaaagtt tgtcggcatc
     2281 gaaaacgtgc atcggctggc cgatttccag cattacataa ttgccgatgt ccaccagcac
     2341 ggaaatactg cggatgccgc tgcgctccaa acgttgtttc atccaatcgg gagtagccgc
     2401 tttcgcattg acattttcaa taacgcggct gataaaacgg ccgcaatcgg caggcgcgtt
     2461 aatctgcacg ggctgttttc gactgcccgt gatcggcgcg gtatggattt cgggctgcct
     2521 gaacgcgcac cccgtcaatg cggacacttc gcgcgcaatg cccttaacac tcaagcaatc
     2581 agcgcggtta ggcgtaattt tcaacgtaaa cagcgtatcg tccaaatcca agtattcacg
     2641 gatattgata ccgacaggcg cgtcttcagg caaaatgtgc aggccgttca caccatcatc
     2701 aggcaaacct agttcattgg tcgaacacag cataccgttt gatggcacgc cgcgcatttt
     2761 ggtcggcttg attttgaaat tacccggcaa aacggcaccc ggcaacgaac acggcacttt
     2821 gatgcccgct ttcacattcg gcgcaccgca cacgatctga accaactctc ccgtacccgc
     2881 atcaactcgg gtaacgttca aacggtctgc atccggatgt ttttcaacgg atttcacttc
     2941 ggcaacaacc acgcccgcaa aggcaggtgc ggcagtttca gcctcttcca cttccaagcc
     3001 ggacatcgtt aacagatgtt ccagcttatc ggcggaaagt tcggtatcgg cttgggtttt
     3061 cagccatgaa taggagaatt gcatgttatg ttctcaatca attaattaac tttagatttt
     3121 caagagctag ctaaaccttt ttgaatggct aagtccctat taattacttc tacagaaatg
     3181 ttccataaag aaatcaaccg caaagtatct ttcttattaa attcttgctt tattaattca
     3241 ttcaattgcc caagataatt aagcggcaca tccgggataa ccagtaataa tttgatttct
     3301 atgctacccc attgcaaatt tgaaaaagca ctaggcataa ttgtttcttt gatatcttct
     3361 ctgcctaata ttcccaaaat ttcaacattt aatgcattac accatttttg ataaatctct
     3421 gaaatatatt catcaaaacg ctcaggagat ctttcttgat ttggaatact ttgttttgcc
     3481 tcaacaaata acaaattatc ttcttgcaga tagataaact ctactgtctt aataccttct
     3541 tgccttctct tccggatata tggttctata taaaacaggt tattttttac tagattaaaa
     3601 gttatacctg attcagtgag atttattatt tcactcataa aaacctaacc aatccatgct
     3661 gtttccggag ataaaacacc tatttcttct ctgaacaatc gaatagactc accaataatt
     3721 tcattatccg gcatgctatt ttgtaaattt tcctgcaccc aagtagtctg ttccttttct
     3781 ttgccaggca taaaaacagg aatactttca ctatgctttc tagctaataa acacagcttc
     3841 ttgataacaa aatatgaatg cgtactcata aaaatctgta ttcccaattt ggatttagat
     3901 aaggaaaaca gaatttctag aaattgatta attgctttcg gatgtagatt agcttctggc
     3961 tcatcaataa ataagattga tccaggactc aaaaaccgat ttcctaatag agtgtctaat
     4021 atcccaatct tttttacccc ctctgctgta gaattgatag agaaagtttg tctatctttt
     4081 tcatataccc atgccttctt tttatcattg tagctaacat aaccgccaaa caatgcttct
     4141 agatcctttc tagcttccgc tgcctcaggg taattcctcc cttttgttgg tttaatatcc
     4201 aatgctttta ccaaatcaat gtaagtagca tcaaaaccaa atctcttttc ttcctctgat
     4261 tttttaatta catcaaatag tgatagaact tctttgggtg gtaaaaaaat agagttagcg
     4321 tttattctgg gagaaagttc gtatagctcc ggtttaatta gctttttggt agttctacca
     4381 aaagaaaaca aaactgaaga actatcttct aaagtcatac ttaacttaag aggccgttcg
     4441 tttggatttt cttttcctct tgtaactaaa tcaccaattt cctctacttg gaacgtccaa
     4501 tatagcttat ctcgcaattc ctcagaaaaa tcacgaatat tctttccttt atgagcttcc
     4561 tcatgacttt ttaaaacact atataatgcc tttaaaataa aagttttccc tttgctattt
     4621 tctgcaataa ttaaatttat tttccctaaa ttatcagcct tcaataagct aatcggcccg
     4681 aaattttcaa gccaaaatga tttaatcata taatccccct aatatatatc aacaatattt
     4741 gtacaagaca cattgtgaaa agacagcaac cacagcacca tagtaaacgc atagcaggcg
     4801 gataatggag cgacatagcg tttcatgatt tagctttctg aggtaggcac aggctacttt
     4861 tttcattttt aggcagccta cacaatcatt ttacaaactg cttcaaaaaa ttcaaatcgt
     4921 tatcaaaaaa caagcgcaaa tcgttcacgt tgtaacgcag catcgcgaaa cggtcgagac
     4981 cgataccgaa agcgaaaccg gtgtattttt cgggatcgat attcacattt ctcaacacgt
     5041 tgggatgcac catgccgcag ccgccgactt ccagccattt gccgttttcg cccataatgt
     5101 cgatttcggc agacggttcg gtgaacggga agaaagacgg gcggaaacgt acttgcaaat
     5161 catcgcgttc aaagaagcga cggataaaat ccgtgaacac tgctttcaaa tcggcaaaag
     5221 ttacgccctc ttctacccac aaaccttccg cctgatggaa cataggcgag tgcgtggcat
     5281 cgctgtccac acggtaaacg cggccggggg cgataatgcg gatgggcggc tcttttttat
     5341 cgagcatata gcggatttga atcggggaag tgtgcgtacg caaaacatcg ccgttttcaa
     5401 cgtaaaacgt atcctgcatc gcacgggcag gatggtttgc agggatgttc agggcttgga
     5461 aattgtgaaa atcgtcttcg atttcaggcc cgtccgccac ttcgaaaccc attccgtgaa
     5521 agagttcgac cacacgttgc aaggtcaggg ttacgggatg caggctgccg ccttcctgag
     5581 cgcgtccggg cagggtaata tcgagggctt cggcggcaag tcgggcttgc agcttgactt
     5641 cgttgagggc ttcgcgtttg gcattaaaag ccgtctgaaa ccggtttttg cattcattga
     5701 tatgcgcacc tatggttttg cgctcttcag gcgacatttg ccccaaagtt ttcagaagtc
     5761 cggtcaactc gccggtttta ccaagataac gggctttgat ttgttctaga gcgttgaagt
     5821 cttgcgcagc ttctactgcg gcaatgcctt ctgcaacgat gcggtttaca ttttccataa
     5881 tatcaagtct gtcaattaat gatataccac ctgaaacaac gaggccgcct gtatagcctg
     5941 acatatcaga ctcttcagac gaccttattt tcttcacggt caatccattg gataaatttt
     6001 ctgtatagtg gattaaatt
//
LOCUS       DAWKOG010000126         5991 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.126, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000126 DAWKOG010000000
VERSION     DAWKOG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5991
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.126"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..410)
                     /locus_tag="WJK66_001648"
     CDS             complement(<1..410)
                     /locus_tag="WJK66_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEZ7579332.1"
                     /translation="MLCPEKMSGMAGRHPYGVRSGLRRNGLKLWDIHFRMTRFIVARC
                     GLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNINAASQQELEALPGIGPAKAKAIAE
                     YRAQNGAFKSVDDLTKVKGIGPAVLAKLKDQASVG"
     gene            complement(446..559)
                     /gene="rrf"
                     /locus_tag="WJK66_001649"
     rRNA            complement(446..559)
                     /gene="rrf"
                     /locus_tag="WJK66_001649"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(656..3547)
                     /locus_tag="WJK66_001650"
     rRNA            complement(656..3547)
                     /locus_tag="WJK66_001650"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3954..4029)
                     /locus_tag="WJK66_001651"
     tRNA            complement(3954..4029)
                     /locus_tag="WJK66_001651"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3994..3996),aa:Ala,seq:tgc)
     gene            complement(4037..4113)
                     /locus_tag="WJK66_001652"
     tRNA            complement(4037..4113)
                     /locus_tag="WJK66_001652"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4077..4079),aa:Ile,seq:gat)
     gene            complement(4215..5755)
                     /locus_tag="WJK66_001653"
     rRNA            complement(4215..5755)
                     /locus_tag="WJK66_001653"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1302 a         1759 c         1280 g         1650 t
ORIGIN      
        1 ccgacagaag cctgatcctt cagcttcgcc agcaccgcag ggccgatgcc ctttaccttg
       61 gtcaaatcgt ctacagactt gaacgcaccg ttttgcgcac ggtattccgc aatggccttc
      121 gccttcgcag ggcctatgcc cggcagcgcc tccagctcct gctgcgaagc cgcattgatg
      181 tttaccgccg caagggagaa ggcgcaggag aacagcatac agaacagcac gaacattttc
      241 ttcatggttt ttcctttaag ggttgcaaac aataaaccgc atcttgcgac gataaaacgg
      301 gtcattctaa aatgaatatc ccaaagtttc aagccgttcc tccgcaaacc cgaccggacg
      361 ccgtacggat gccgtcccgc catccccgac attttttccg ggcaaagcaa aaacccccga
      421 ataatcgggg gttttctgaa tgggtgtttg gcagtgacct actttcgcat ggaagaacca
      481 cactatcatc ggcgctgagt cgtttcacgg tcctgttcgg gatgggaagg cgtgggacca
      541 actcgctatg gccgccaaac ttaaactgtt acaaatcggt aaagccttaa tcaatatatt
      601 cggtaatgac tgaatcagtc agtaagcttt tatctcttga agttcttcaa atgatagagt
      661 caagcctcac gagcaattag tatgggttag cttcacgcgt taccgcgctt ccacacccca
      721 cctatcaacg tcctggtctc gaacgactct ttagtgcggt taaaccgcaa gggaagtctc
      781 atcttcaggc gagtttcgcg cttagatgct ttcagcgctt atctcttccg aacttagcta
      841 cccggctatg caactggcgt tacaaccggt acaccagagg ttcgtccact ccggtcctct
      901 cgtactagga gcagcccccg tcaaacttcc aacgcccact gcagataggg accaaactgt
      961 ctcacgacgt tttaaaccca gctcacgtac cactttaaat ggcgaacagc catacccttg
     1021 ggaccgacta cagccccagg atgtgatgag ccgacatcga ggtgccaaac tccgccgtcg
     1081 atatgaactc ttgggcggaa tcagcctgtt atccccggag taccttttat ccgttgagcg
     1141 atggcccttc catacagaac caccggatca ctatgtcctg ctttcgcacc tgctcgactt
     1201 gtcggtctcg cagttaagct accttttgcc attgcactat cagtccgatt tccgaccgga
     1261 cctaggtaac cttcgaactc ctccgttacg ctttgggagg agaccgcccc agtcaaactg
     1321 cctaccatgc acggtccccg acccggatga cgggtctggg ttagaacctc aaagacacca
     1381 gggtggtatt tcaaggacgg ctccacagag actggcgtct ctgcttctaa gcctcccacc
     1441 tatcctacac aagtgacttc aaagtccaat gcaaagctac agtaaaggtt cacggggtct
     1501 ttccgtctag cagcgggtag attgcatctt cacaaccact tcaacttcgc tgagtctcag
     1561 gaggagacag tgtggccatc gttacgccat tcgtgcgggt cggaacttac ccgacaagga
     1621 atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct tcgatccgat
     1681 gctctcacat cttcaattaa ccttccagca ccgggcaggc gtcacaccct atacgtccac
     1741 tttcgtgtta gcagagtgct gtgtttttaa taaacagtcg cagccaccta ttctctgcga
     1801 ccctccgggg cttacggagc aagtccttaa ccttagaggg cataccttct cccgaagtta
     1861 cggtatcaat ttgccgagtt ccttctcctg agttctctca agcgccttag aattctcatc
     1921 ctgcccacct gtgtcggttt gcggtacggt tcgattcaaa ctgaagctta gtggcttttc
     1981 ctggaagcgt ggtatcggtt gcttcgtgtc cgtagacact cgtcgtcact tctcggtgtt
     2041 aagaagaccc ggatttgcct aagtcttcca cctaccggct taaacaagct attccaacag
     2101 cttgccaacc taaccttctc cgtccccaca tcgcatttga atcaagtaca ggaatattaa
     2161 cctgtttccc atcgactacg catttctgcc tcgccttagg ggccgactca ccctacgccg
     2221 atgaacgttg cgcaggaaac cttgggcttt cggcgagcgg gcttttcacc cgctttatcg
     2281 ctactcatgt caacattcgc acttctgata cctccagcac actttacaat gcaccttcat
     2341 cggcctacag aacgctcccc taccatgccg gtaaaccggc atccgcagct tcggttatag
     2401 atttgagccc cgttacatct tccgcgcagg acgactcgac cagtgagcta ttacgctttc
     2461 tttaaatgat ggctgcttct aagccaacat cctggctgtc tgggccttcc cacttcgttt
     2521 accacttaat ctatcattgg ggaccttagc tggcggtctg ggttgtttcc ctcttgacaa
     2581 cggacgttag cacccgctgt ctgtctcccg aggaaccact tgatggtatt ctgagtttgc
     2641 catgggttgg taagttgcaa taacccccta gccataacag tgctttaccc ccatcagtgt
     2701 cttgctcgag gcactaccta aatagttttc ggggagaacc agctatctcc gagtttgttt
     2761 agcctttcac ccctatccac agctcatccc cgcattttgc aacatgcgtg ggttcggtcc
     2821 tccagtacct gttacggcac cttcaacctg gccatggata gatcactcgg tttcgggtct
     2881 acacccagca actcatcgcc ctattaagac tcggtttccc tacgcctccc ctattcggtt
     2941 aagctcgcta ctgaatgtaa gtcgttgacc cattatacaa aaggtacgca gtcacacctc
     3001 tagggcgctc ccactgtttg tatgcatcag gtttcaggtt ctgtttcact cccctcccgg
     3061 ggttcttttc gcctttccct cacggtactg gttcactatc ggtcgatgat gagtatttag
     3121 ccttggagga tggtcccccc atattcagac aggatttcac gtgtcccgcc ctacttttcg
     3181 tacgcttagt accactattg agatttcgaa tacgggacta tcacccacta tggtcaagct
     3241 tcccagcttg ttcttctatc tcgacagtta tcacgtacag actcctccgc gttcgctcgc
     3301 cactacttgc ggaatctcgg ttgatttctt ttcctccggg tacttagatg gttcagttct
     3361 ccgggttcgc ttctctaagt ctatgtattc aacttaggat actgcacaga atgcagtggg
     3421 tttccccatt cggacatcgc ggaatcattg ctttattgcc agctcccccg cgcttttcgc
     3481 aggcttacac gtccttcgtc gcctatcatc gccaaggcat ccacctgatg cacttattca
     3541 cttgactcta tcatttcaag aacttctttg actttgccta acattccgtt gactagaaca
     3601 tcagacttga atttcctact ttgataaagc ttactgcttt gttgtgtctt aatcctgcct
     3661 tttgtgtttc aggattaagt cgatacaatc atcacccaaa tactgtgttt gttttctttt
     3721 ctcttgtgag cgatttttta tcctttgcaa agaataaaaa atcaaaacaa acgctttgtc
     3781 tttgtttgtt gatttcggct ttccaatttg ttaaagatcg atgcgttcga tattgctatc
     3841 tactgtgcaa atcaaaacga gctgattatt atatcagcat tttgttcttg gtcaagtgtg
     3901 acgtcgccct gaatggattc tgttccattc ttccgttttg atttgtacag tattggtgga
     3961 ggcaaacggg atcgaaccga tgaccccctg cttgcaaagc aggtgctcta ccaactgagc
     4021 tatgcccccc cgttcttggt gggtctggga ggacttgaac ctccgacccc acgcttatca
     4081 agcgtgtgct ctaaccagct gagctacaaa cccggattct cttcttaagc gaatcttgcc
     4141 ttcactcaag cttcttccgc atctttttca gtttaccgat aagtgtgaat gcctaaagcc
     4201 tcttctttct ctagaaagga ggtgatccag ccgcaggttc ccctacggct accttgttac
     4261 gacttcaccc cagtcatgaa gcataccgtg gtaagcgggc tccttgcggt taccctacct
     4321 acttctggta tcccccactc ccatggtgtg acgggcggtg tgtacaagac ccgggaacgt
     4381 attcaccgca gtatgctgac ctgcgattac tagcgattcc gacttcatgc actcgagttg
     4441 cagagtgcaa tccggactac gatcggtttt gtgagattgg ctccgcctcg cggcttggct
     4501 accctctgta ccgaccattg tatgacgtgt gaagccctgg tcataagggc catgaggact
     4561 tgacgtcatc cccaccttcc tccggcttgt caccggcagt ctcattagag tgcccaactg
     4621 aatgatggca actaatgaca agggttgcgc tcgttgcggg acttaaccca acatctcacg
     4681 acacgagctg acgacagcca tgcagcacct gtgttacggc tcccgaaggc actcctccgt
     4741 ctccggagga ttccgtacat gtcaagacca ggtaaggttc ttcgcgttgc atcgaattaa
     4801 tccacatcat ccaccgcttg tgcgggtccc cgtcaattcc tttgagtttt aatcttgcga
     4861 ccgtactccc caggcggtca atttcacgcg ttagctacgc taccaagcaa tcaagttgcc
     4921 caacagctaa ttgacatcgt ttagggcgtg gactaccagg gtatctaatc ctgtttgcta
     4981 cccacgcttt cgggcatgaa cgtcagtgtt atcccaggag gctgccttcg ccatcggtat
     5041 tcctccacat ctctacgcat ttcactgcta cacgtggaat tctacctccc tctgacacac
     5101 tcgagtcacc cagttcagaa cgcagttccc aggttgagcc cggggatttc acatcctgct
     5161 taagtaaccg tctgcgcccg ctttacgccc agtaattccg attaacgctc gcaccctacg
     5221 tattaccgcg gctgctggca cgtagttagc cggtgcttat tcttcaggta ccgtcatcag
     5281 ccgctgatat tagcaacagc cttttcttcc ctgacaaaag tcctttacaa cccgaaggcc
     5341 ttcttcagac acgcggcatg gctggatcag gcttgcgccc attgtccaaa attccccact
     5401 gctgcctccc gtaggagtct gggccgtgtc tcagtcccag tgtggcggat catcctctca
     5461 gacccgctac tgatcgtcgc cttggtaggc ctttacccca ccaactagct aatcagatat
     5521 cggccgctcg aatagcgcaa ggcccgaaga tcccctgctt tctctctcaa gacgtatgcg
     5581 gtattagctg atctttcgat cagttatccc ccactactcg gtacgttccg atatgttact
     5641 cacccgttcg ccactcgcca cccaagaagc aagcttctct gtgctgccgt ccgacttgca
     5701 tgtgtaaagc atgccgccag cgttcaatct gagccaggat caaactctta tgttcaatct
     5761 ctaacttttt aacttctggt ctgcttcaaa gaaaccaaca ggacaatgtt caaaacatta
     5821 tcttgtctgt ctttcaaaca gtgtgaggct caaggcactc acacttatcg gtaatctgtt
     5881 ttgttaaaga gcgtttgaat tataaagtat ttcttccgcc tgtcaagata tctctcgata
     5941 tccccaacat tctgtgctat acttttcagt tcgtccgcca cttctgcagc a
//
LOCUS       DAWKOG010000127         5967 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.127, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000127 DAWKOG010000000
VERSION     DAWKOG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.127"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            11..187
                     /locus_tag="WJK66_001654"
     CDS             11..187
                     /locus_tag="WJK66_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579333.1"
                     /translation="MAHQAPHCVLPERDYPPIRFYRYKQTDFNRKGMGIKSVSDTSRA
                     MPSENQSPLSDGIV"
     gene            264..1211
                     /locus_tag="WJK66_001655"
                     /pseudo
     CDS             264..1211
                     /locus_tag="WJK66_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
     gene            complement(1364..2488)
                     /gene="rluD"
                     /locus_tag="WJK66_001656"
     CDS             complement(1364..2488)
                     /gene="rluD"
                     /locus_tag="WJK66_001656"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEZ7579334.1"
                     /translation="MQNTSFDNESDYSDDSDFASASETENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MALDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQVPRAGIVHRLD
                     KETSGLMVVAKTLSAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLTHSYIECSLETGRTHQIRVHMREANHPLAADPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            2487..3290
                     /locus_tag="WJK66_001657"
     CDS             2487..3290
                     /locus_tag="WJK66_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEZ7579335.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(3348..5567)
                     /locus_tag="WJK66_001658"
     CDS             complement(3348..5567)
                     /locus_tag="WJK66_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEZ7579336.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWWAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPSAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTALRGQWAASVLSLVLLGYLFASLPLVSPLVNA
                     VAIPWFSWVLTPLALLGSVVPFAPLQQLGAFLAEYTLRFLVWLADVSPEFAVASAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQMGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         1465 a         1884 c         1438 g         1180 t
ORIGIN      
        1 tcaacagggg atggcacatc aagcaccgca ctgcgtgttg cccgaacgag actaccctcc
       61 gattagattc tatcgctata aacagacgga tttcaaccgg aaaggaatgg ggataaagtc
      121 cgtttccgac acctctcggg cgatgccgtc tgaaaaccaa tctccacttt cagacggcat
      181 tgtttaggca cggtcatttt gcaaattaag gttaaggcgt gtatgcttgc cttcccttaa
      241 aaacaaacac gcaacgcatt attatgaata tcctcagtaa aatcagcagc tttatcggaa
      301 aaacattttc cctctgggcc gcgctctttg ccgccatcgc ttttttcgcg cccgacacct
      361 tcaaatgggc ggggccttat attccttggc tgttgggcat tattatgttc ggtatgggtt
      421 tgacgctcaa accttccgac ttcgatattt tgttcgaaca tcccaaagtc gtcatcatcg
      481 gcgtaatcgc acaattcgcc attatgccgg caaccgccta gctgctgtcc aaactgttga
      541 acctgcctgc cgaaatcgcg gtcggcgtga ttttggtcgg ctgctgcccg ggcggtacgg
      601 cttccaatgt gatgacctat ctggcgcgcg gcaatgtggc tttgtcggtt gccgttacgt
      661 ctgtttccac cctgatttcc ccattgctga ctcccgccat cttcctgatg cttgccggtg
      721 aaatgctgga aatccaagcg ggcagtatgt tgatgtccat cgtcaaaatg gttttgctcc
      781 ccattgtttt gggtttgatt gtccataagg ttttgggcag taaaaccgaa aagctgaccg
      841 atgcgctgcc gctggtttcc gttgccgcca tcgtgctgat tatcggcgcg gttgttgggg
      901 caagcaaagg caagattatg gaaagcggcc tgctgatttt tgcggttgtc gtactccaca
      961 acggcatcgg ctacctgctc ggcttctttg ccgccaaatg gaccggcctg ccttatgatg
     1021 cacaaaaaac gctggccatc gaagtcggta tgcaaaactc tggcctggcc gccgcgcttg
     1081 ccgccgcaca ctttgccgcc gcgccggtcg ttgccgttcc gggcgcattg ttcagcgtgt
     1141 ggcacaatat ctccggctcg ctgctggcaa cttattgggc ggccaaagcc ggtaaacata
     1201 aaaaacccta aacgcctttc atcataatgc cgtctgaacg gggcaaacga aaccggcggt
     1261 ttccgcccga ccccttcaga cggcctttgt ttgaaatcgg cggggacgat tgcccggctg
     1321 ctccggttcg gctgcctgcg ttcgccccgc cttgcaaacc gcctcactcc cttacataaa
     1381 ccacttcgac atcgtagtcg tcttcgttcc aatcgtcatc gtcgtccgcg ccgaatttgt
     1441 cctgccattc ctcttcattg ctcaaagacg aatccaaacc ggcttcaaga cggaggacag
     1501 acaacaaatg atatatgtcg tccggaatcg gtgcctcaaa cgaaacggtt tcgccgcttt
     1561 ccggatgggt gaaactcaag cggtaggcgt gcaacgcctg acgcgcgccc aaacttttaa
     1621 cggcttcttt caccgtgtcg ccgcacggat ggcgcgggtt gccgtaaacc gggtcggcgg
     1681 caagcggatg gttggcctcg cgcatatgga cgcggatttg gtgcgtcctg cccgtttcga
     1741 gcgagcattc gatatagctg tgggtaagat agcgttccaa cactttgacg tgggtaacgg
     1801 ctggtttgcc gccgaatttg acgactgcca ttttcaggcg gttgtgcgga tcgcgtccga
     1861 tttgggtttc gattttaccg tcaaagggga cgatgccgtt ggcgacggcg cggtagatgc
     1921 gtttgaccgt gcgctcttga agctgcctca cgagggaatt ttgcgccgac agggttttgg
     1981 caaccaccat cagcccgctg gtttccttgt ccaaacggtg tacgatgccc gcgcgcggta
     2041 cttggctcaa ttcgggacag tgcgccaaca ggccgttgag cagcgtcccc gtccagttgc
     2101 ccgccgccgg atgcaccacc agtccggccg gtttgttgac gacgatgacg gtatcgtctt
     2161 cgtaaacaat atccaaagcc ataggctctg gaacaaacgc cagattttcc tcactcggac
     2221 ggacggttac acaaatttgc tcgccgccta tcattttgtc tttgggttgc gaaggtttat
     2281 cgtttacaat aaccgcgcct tctttaatcc atgatgtcag gcggctgcgc gagtagtcgg
     2341 gcagaagttt cgccaacacc gcatccaacc gcccgccggc aagctcgagc ggaacggtca
     2401 gaccgatacg gttttcagtt tcggaagctg acgcaaagtc tgaatcgtcg ctataatcgg
     2461 attcattatc aaaggaagta ttctgcatga aaaaaattct tttaacggtt tcattaggtt
     2521 tggcactgag tgcctgtgcc actcaaggta cggtcgataa agatgctcag attacccaag
     2581 attggagtgt ggagaagctc tatgccgaag cccaggacga attgaacagc agcaattata
     2641 cgcgggctgt caagttatac gaaatcttgg aatcgcgctt ccccaccagc cgccatgccc
     2701 ggcaatccca actggatacc gcatacgcct attataaaga cgatgaaaaa gacaaggctc
     2761 tggcggcaat cgaacgcttc cgccgcctcc atccgcagca tccgaatatg gattacgcgc
     2821 tgtatctgcg cggcttggtg ctgttcaacg aagaccagtc cttcttgaac aaactggcct
     2881 cgcaagactg gtccgaccgc gacccgaaag ccaaccgcga agcgtaccag gcgtttgcgg
     2941 aactcgtcca acgcttcccg aacagcaaat acgccgccga tgcaaccgca cgcatggtca
     3001 aactggtcga tgcactgggc ggcaatgaaa tgtcggtggc gcgttactac atgaaacgcg
     3061 gcgcatatat cgccgccgcc aaccgcgccc aaaaaattat cggcagctat caaaatacac
     3121 gctatgtcga agaatcgctc gccatattgg aacttgccta ccaaaaactc ggcaaaccgc
     3181 agcttgccgc cgatacgcgc cgcgtgttgg aaaccaactt cccgaaaagc ccgtttttga
     3241 cgcacgcctg gcagcccgac gatatgccct ggtggcgtta ctggcattaa gcccatcttc
     3301 gtaatgccgt ctgaagccgt ttcagacggc attttatgtt tcaaacctca ttcaaacggt
     3361 tttttctgcc aatagaattt ataccctctc aaacgttgag ccttcacgcc gccgcgtccc
     3421 aagccgaatt gcagtgcgcc cgacaaatcg gtacgcagca gtttgatgcc gtgtgcgcgg
     3481 acacggttct gcaccgcttc ggtcggatgt ttgtaggcgt tggcataacc gcttgaagca
     3541 acggcatatt cgggcgagac ggcattgagg aacacgcccg acgaggacgt attgctgccg
     3601 tgatgtccca aaaccaaaac ctggctgtac aggttgcctc catacttgcc gaccaggctt
     3661 tcctcgccct tcgtatccaa atcgcccgtt accagcagtg ccgcaccgcc cgccacaaca
     3721 cgcaaaacac aacttttccc attatcatcg atgtttttgc gttcagacgg catcaaaaac
     3781 tcaaaatcca cgccgtccca ttgccaacgc tgttccgcac aatgccgcgc cccctcataa
     3841 aattccggct gtccggcata aatcccgccg ttcggtatct tgccgactgc ctgaaaaccg
     3901 ccgtcgtggt cgctgtcgtg atgcgacaga accagcttgt ccaaacggcg gacacccgcc
     3961 gcattcaaac tcggcacaat ccccatctgc gccgccgctg ccgttccggt gtcaaacaaa
     4021 agatgatgat ttgccgtctg aaccgacacc gacaaaccct gccccgcatc ccaaaccgta
     4081 accgcaacct cattttccgg cacgccgggt gaacggtaaa acacaaaccc tgccaacagc
     4141 aacaccgccc acggacgcaa acccaagccg cgcggcagca gcaacagcaa agcggcacac
     4201 accgccaaca ccaacagcgg caaaggtgcg gaggcaacgg caaactcggg cgacacatcg
     4261 gcaagccaca ccaaaaaccg caaagtatat tccgccaaaa atgcccccaa ctgttgcaaa
     4321 ggcgcaaacg gcacgaccga acccagcaac gccagcggcg tcaataccca agaaaaccac
     4381 ggaattgcca ccgcattgac caaagggctg accaaaggca gcgaagcaaa cagataaccg
     4441 agcaaaacca gcgacaacac cgaagccgcc cactgcccgc gcaaagcagt ttgccgtttt
     4501 ccctcgtgca aacgccccga gcacgcccat atcagggccg ccaccaaacc gaaagacagc
     4561 caagtcccca cacccaagac cgccgacggg tcgaacagca gcaccgccgc caacgcctgc
     4621 caccacgtcg cccacgccga caatcttccc ctgcgccaag cccacgcaaa cgccgccaac
     4681 atcaaaacgc tgcgctgcgt cggcacggaa aaaccggcaa gcagcgcgta aaacagcgcg
     4741 cctgcacacc ccgccgccaa aacccacgcg cgcggccggg caggaaggcg cggggaacag
     4801 gcaagcagcc gcttcgccag ccacgcaaac attaccgcca ccatcgtaac gtgcaaaccc
     4861 gaaatactga ccaaatgcgt cagccccaac ggccggaacg cctgccacaa ttcggggcgc
     4921 aatgccgact gttcgcccac gctcaacgcg cgcatcagcc cgatgccgtc tgaaagcccg
     4981 ccgtccgcat ccgcctgctg ccaattacgg ctgatgcggc tgcgccaaac cgcaatcccc
     5041 caaccgcttc cgccatgcag caaaaccctg tccgcaccga ccgtccccgc gccgcctatt
     5101 ccgttggata atgcccacgc ctcacggttc aaaccgcgca ggttcaactc cccgacgaca
     5161 gggtgcacac gtgccgttat ccgccatctg ctcccgaccg cccattcgcg ccgtttgtag
     5221 tccgacagca gcaaatcaaa cgtccgacca ccgctgtcca cagcctttgc cgcaaactgc
     5281 acacgccgcc cgtccgacct cggcatatcc gccacttcca ccgtcaaagg cacgcctgaa
     5341 acggcttctg cccaccattg ggaagacaat gccgcctccg tcctgaatac gccgtaagcc
     5401 gcgcccgcca acacgcacag catcagaccg gcaaacgcaa aacgccttgc aaacacagca
     5461 aaaaccgcaa aagccgccag ccaaaacagc caatgcggca cgaccggcag cgcgaacgaa
     5521 gccgccgcac cgacaaccca acacggcaaa aaccaacgac ggaacacgtc tccccacccc
     5581 agatatttca aaaataaacg gataataaag caaatccgaa aaaccgtcat aacaagattt
     5641 gaaaatgata tagtggttta aatttaaatc aggacaaggc gacgaagccg cagacagtac
     5701 aagcagtacg acaaggcgag gcaacgctgt actggttttt gttaaaccac tataagaatg
     5761 caaacgaaaa gcctcagtat tatccgagac aacggcaaac tttccatcgt cattcccgca
     5821 caagcgggaa tccaaaaaca aaaagccgca gaaatttaac ggaagaacca aaccccgaaa
     5881 accggctccc cgccttcgct tgaataacag aacaaaggaa tcagtattat ccgagacaac
     5941 cgcaaacttt ccatcgtcat tcccgca
//
LOCUS       DAWKOG010000128         5928 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.128, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000128 DAWKOG010000000
VERSION     DAWKOG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5928
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.128"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            55..1593
                     /gene="murJ"
                     /locus_tag="WJK66_001659"
     CDS             55..1593
                     /gene="murJ"
                     /locus_tag="WJK66_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEZ7579337.1"
                     /translation="MNMLGALVKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAKDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFSIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LSFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPG
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGMAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLAPGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHAGLSLAIGLGACINAGLLFFLLRRHGIYRPGKGWAAFLAKMLL
                     SLAVMGGGLWAAQACLPFEWVRAGGMRKAGQLCILIAVGGGLYFASLAALGFRPRHFK
                     RVEN"
     gene            1725..2681
                     /locus_tag="WJK66_001660"
     CDS             1725..2681
                     /locus_tag="WJK66_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HEZ7579338.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQRSAPAYRRELLPDSTGKT
                     KTAYDFSDGISPDAPLVVLFHGLEGGSGSHYAVELMLAVRDRGWNGVVVHFRSCGGVA
                     NTAPVFYHLGDTAEIAFTLDTLAARYREIYAVGVSLGGNALAKYLGEQGENALPQAAA
                     VISAPVDAEAAGNRFDSGITRLLYTRYFLRTLIPKARSLQGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAVNDPFLPPEALPRADEVSEAV
                     TLFQPTHGGHVGFVGSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            2732..3514
                     /gene="trpC"
                     /locus_tag="WJK66_001661"
     CDS             2732..3514
                     /gene="trpC"
                     /locus_tag="WJK66_001661"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEZ7579339.1"
                     /translation="MTDILNKILTAKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIGS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPPEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEREQLERFEAVA
                     HELGMTVLLELHDESELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMQSRGVHTFLIGETFMRADNIEAEVGKLF"
     gene            3580..5880
                     /gene="recQ"
                     /locus_tag="WJK66_001662"
     CDS             3580..5880
                     /gene="recQ"
                     /locus_tag="WJK66_001662"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEZ7579340.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGRQEAVINTLAGGGSLTVLMPTGG
                     GKSLCYQIPALMREGVAVVVSPLIALMNDQVASLHVAGIEAAAVNSGTSADEAREIAD
                     KLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGM
                     LAERYPNVPRIALTATADAATRADIKHYLHLDDAPEFVSSFDRPNIYYQVIEKNNGKK
                     QLLEFIRKEMTGQSGIVYCLSRKKVEDVARFLRENGLNAIPYHAGLGMDVREENQRRF
                     TREDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAASWLCY
                     GLNDWVLLRERIAEGNSGEVQKQIEMQKLDAMLAVCETAACRRVLLLKHFGEASEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQLS
                     TFGIGAELSDKEWRSVIRQCISLGYLTVNIARYQALQLTEAAKKVLKGKTGVMLRPLK
                     RNKPAVRTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKMP
                     QNLNGLHDIYGLGEAKTERFGHGILEVCRNAAGFSRDAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLAATPETETDLEGVHGLGSVRAAKYGRDILAVCRP
                     FSDGIDEAAKRKRRLMRALIQWCGETAKHEQSEPYRILSKAALRAIAAKQPKGLAELA
                     AVYGVGEEKTARYGAAVLAVLGQNAV"
BASE COUNT         1274 a         1768 c         1630 g         1256 t
ORIGIN      
        1 aaagtctgat cctgccgccc taaagggcgg ggtttcaacc gaaaaggaaa tacgatgaat
       61 atgctgggag ctttggtaaa agtcggcagc ctgacgatgg tgtcgcgcgt tttgggattt
      121 gtgcgcgata cggtcattgc gcgcgcattc ggcgcaggca tggcgacgga tgcgttcttt
      181 gtcgcgttca aactgcccaa cctgcttcgc cgcgtgtttg cggagggggc gtttgcccaa
      241 gcgtttgtgc cgattttggc ggaatataag gaaacgcgtt ctaaagaggc gacggaggct
      301 tttatccgcc atgtggcggg gatgctgtcg tttgtactgg tcatcgttac cgcgctgggc
      361 atacttgccg cgccttgggt gatttatgtt tccgcacccg gttttgccaa agatgccgac
      421 aaatttcagc tctctatcga tttgctgcgg attacgtttc cttatatctt attgatttca
      481 ctttcctctt ttgtcggctc ggtactcaat tcctatcata aattcagcat tcctgcgttt
      541 acgcccacgt tcctgaacgt gtcgtttatc gtattcgcgc tgtttttcgt gccgtatttc
      601 gatcctcccg ttaccgcgct ggcttgggcg gtttttgtcg gcggcatttt gcaactcggc
      661 ttccaactgc cctggctggc gaaactgggt tttttgaaac tgcccaaact gagtttcaaa
      721 gatgcggcgg tcaaccgcgt gatgaaacag atggcgcctg cgattttggg cgtgagcgtg
      781 gcgcagattt ctttggtgat caacacgatt ttcgcgtctt atctgcaatc gggcagcgtt
      841 tcatggatgt attacgccga ccgcatgatg gaactgcccg gcggcgtgct gggggcggca
      901 ctcggtacga ttttgctgcc gactttgtcc aaacactcgg caaaccaaga tacggaacag
      961 ttttccgccc tgctcgactg gggtttgcgc ctgtgcatgc tgctgacgct gccggcggcg
     1021 gtcggaatgg cggtgttgtc gttcccgctg gtggcaacct tgtttatgta ccgtgaattc
     1081 acgctgtttg acgcgcagat gacgcaacac gcgctgattg cctattcttt cggtttaatc
     1141 ggtttgatca tgattaaagt gttggcgccc ggcttctatg cgcggcaaaa catcaaaacg
     1201 cccgtcaaaa tcgccatctt cacgctcatc tgcacgcagt tgatgaacct tgcctttatc
     1261 ggcccgttga aacacgccgg gctttcgctc gccatcggtt tgggcgcgtg catcaatgcc
     1321 ggattgttgt tcttcctgtt acgcagacac ggcatttacc ggcccggcaa gggttgggcg
     1381 gcgttcttgg cgaaaatgct gctctcgctc gccgtgatgg gcggcggact gtgggcggcg
     1441 caggcttgcc tgccgtttga atgggtacgc gccggcggaa tgcggaaagc ggggcagctc
     1501 tgcatcctga ttgccgtcgg cggcggactg tatttcgcat cactggcggc tttgggcttc
     1561 cgtccgcgcc atttcaaacg cgtggaaaac tgaccgatgc cgatatcctt tttcggcaaa
     1621 cgcttttcca cgcctatatt cgggcgcgca tcccccacgt ccgcaccagc cggacgggct
     1681 gccgcatcat caatgccgtc tgaaaacaag aaaaccgata ccttatgatt ttgacaccgc
     1741 cggacacacc ctttttcctc cgcaacggca atgccgacac gattgccgcc aaattcctgc
     1801 aacgctccgc acctgcatac cgccgcgagc tgcttcccga cagcacgggt aaaaccaaaa
     1861 ccgcctacga cttttcagac ggcatttcgc ccgatgcgcc gctggtcgtg ctgtttcacg
     1921 gtttggaggg cggcagtggc agccattacg cggtcgaact gatgctcgcg gtacgcgatc
     1981 ggggttggaa cggcgtagtc gtccatttcc gcagctgcgg cggcgtagcg aacaccgccc
     2041 cggtgttcta ccacttgggc gataccgccg aaattgcctt tactttggac acgctcgccg
     2101 cgcgttaccg tgaaatatac gccgtcggcg tatcgctggg cggcaacgcg ctggcaaaat
     2161 atttgggcga acagggcgaa aacgcgctgc cgcaagccgc cgccgtcatc tccgcacccg
     2221 tcgatgcaga ggcggcaggc aaccgcttcg acagcggcat cacacggctg ctctacacgc
     2281 gctacttcct ccgcacactg atacccaaag cacggtcgct ccaaggtttt cagacggcat
     2341 ttgccgcagg gtgcaaaaca ctgggcgagt ttgacgaccg tttcaccgca ccgctgcacg
     2401 gctttgccga tcggcacgac tactaccgcc aaacttcctg caaaccgctg ctcaaacacg
     2461 ttgccaaacc gctgctcctg ctcaatgccg tcaacgaccc cttcctgccg cccgaagcgc
     2521 tgccccgcgc agacgaagtg tccgaagccg ttaccctgtt ccagccgaca cacggtggtc
     2581 atgtcggctt tgtcggcagc accggcggca ggctgcacct gcaatggttg ccgcagaccg
     2641 tcctgtccta tttcgacagc ttccgcacaa acaggcgtta acggtttgat gctaatattc
     2701 cccctttccc cagacgaata cggaacacga catgaccgac atcctcaata aaatccttac
     2761 cgccaaggca caggaagttg ccgcccaaaa agccgccgtc aacgccgaac acatccgcgc
     2821 acttgccgca gaagccgcgc ccgtccgcag ctttatcggt tcgatacgcg gcaaacaccg
     2881 cctcaacctg cccgccgtca ttgccgaaat caaaaaggca agcccgagca aagggttaat
     2941 ccgtccggac ttccgaccgc ccgagattgc acgcgcctat gaaaacgccg gggcggcgtg
     3001 tttgtccgta ctgaccgacg aaccctattt ccaaggttcg cccgaatacc tcaaacaggc
     3061 gcgcgaagcc gtatcgctgc ccgtgctgcg caaagacttc atcatcgacg aataccaggt
     3121 ttatcaggcg cgcgcctggg gggcggatgc cgtcctgctg attgccgccg cactggaacg
     3181 ggaacaattg gaacgctttg aagcggtggc gcacgaattg ggcatgaccg tcctgctcga
     3241 gctgcacgat gaaagcgaat tggaaaaatg ccgcaacctg accacgccgt tgtggggtgt
     3301 aaacaaccgc aacctgagga cttttgaagt ctccctcgac caaaccctgt cgctgctgcc
     3361 cgcgctggaa ggcaaaaccg tcgttaccga aagcggcatt acaggcaagg cggatgtgga
     3421 atttatgcaa tcgcgcggcg tgcatacctt cctgatcggc gaaacgttta tgcgtgccga
     3481 caatattgag gcggaagtag gcaaactctt ctaaatcccg atttcagacg gcattccctt
     3541 tctttaaccg acacaccctt taccggacac ggaaccatca tgcaccgacc gaccgccaaa
     3601 cagattctgc acgaagtatt tggttatccc gaatttcgcg gcaggcagga ggctgtcatc
     3661 aatactttgg caggcggcgg gagtttgacg gtgctgatgc cgacgggcgg gggcaagtct
     3721 ttgtgttacc agattcccgc gctgatgcgc gaaggcgtgg cggttgtcgt atcgccgctg
     3781 attgcgctga tgaacgacca agtggccagc ctgcatgtgg ccgggattga agcagcggca
     3841 gtcaacagtg gtacatcggc agatgaggcg cgcgagattg ccgacaagct tgcccaaggc
     3901 cgtctgaagc tgctttatgt cgcaccggaa cgcctagtta ccgaccgctt tttacgtttt
     3961 ctcgaccaac aaaccgtcag cctgtttgcc attgatgagg cgcattgcgt cagccagtgg
     4021 ggacacgatt tccgccccga ataccaacag ctcggcatgc ttgccgaacg ctatccgaac
     4081 gtcccgcgca tcgccctgac cgctaccgcc gatgccgcca cgcgcgccga catcaagcat
     4141 tatctgcact tggacgacgc gcccgaattt gtctccagct ttgaccgccc gaatatttat
     4201 tatcaggtta tcgaaaaaaa caacggcaaa aaacaactgc ttgaattcat ccgcaaagaa
     4261 atgacggggc aaagcggcat tgtgtattgc ttaagccgca aaaaggttga agatgtggcg
     4321 cggtttttgc gtgaaaacgg attaaacgcg attccgtatc acgccggttt gggtatggac
     4381 gtgcgtgagg aaaaccaacg ccgctttacg cgtgaagaca atattatcgt ggtggcgacc
     4441 gtggcgttcg gtatgggcat agacaaaccc gacgtgcgct ttgtcgccca tctcgatatg
     4501 ccccagagtg tcgaacattt ctatcaggaa tcggggcgcg ccggccggga cgggctgcct
     4561 gccgcgagct ggctgtgtta cggcttgaac gattgggtgt tgctgcgcga acggattgcc
     4621 gaaggcaaca gcggcgaagt gcaaaagcaa atcgaaatgc aaaaactcga tgccatgctt
     4681 gccgtctgcg aaaccgccgc ctgccgccgc gtgctgctgc tcaaacattt cggcgaagca
     4741 tccgaaccct gcggccattg cgacaactgc ctgcatccgc ccgtacggtt tgacggcacg
     4801 gtgttggtgc aaaaattact cagctgcgtg taccgcgccg gacaacgttt tgccgccggt
     4861 tacatcacca accttttgcg cggtaaaagc gacgattgga tacgcggcaa ccggcacgaa
     4921 caactgtcca cattcggcat cggtgcagaa ttgtccgaca aagaatggcg cagcgtcatc
     4981 cgccagtgta tcagcctcgg ctacctcacc gtcaacattg cccgatatca ggcattgcaa
     5041 ctgaccgaag ccgccaaaaa agtcctcaaa ggcaaaaccg gagtgatgct gcgtccgctc
     5101 aaacgcaaca agcccgccgt ccgcaccctc aaagacaact ggctgcgtac cgaacgcgaa
     5161 gaacgcctgt ggcaggcatt gcgcgtttgg cgtatgaaac aggcagaagc cgaaggcatc
     5221 cccgcctata tgattttcgg cgacaaaacc ctgcgcgacc ttgtcgaaaa aatgccgcaa
     5281 aacctcaacg ggctgcacga catctacggc ttgggcgaag ccaaaaccga acgtttcgga
     5341 cacggcatac tcgaagtctg ccgcaacgcc gccggcttca gtcgcgacgc ggtcatccgt
     5401 ccgcaaaccg aacgcgaaca acaactgcgt caaaaactcg aggcctggcg gtatgaacag
     5461 gcaagggcgg aaaactgcgc cctgcatgcc gtcctctccg acgaaagcct tgccgatatg
     5521 cttgccgcca cgcccgaaac cgaaaccgac ctcgaaggcg tacacggctt gggcagcgta
     5581 cgcgccgcca aatacggacg ggacatcctc gccgtctgcc gtccgttttc agacggcatc
     5641 gatgaagccg ccaaacgcaa acgccgcctg atgcgcgccc tgatccaatg gtgcggcgaa
     5701 acggcaaaac acgaacagtc cgaaccctac cgcattctca gcaaagccgc cctgcgcgcc
     5761 attgccgcca aacagccgaa gggtttggcg gagcttgccg ccgtatacgg cgtgggcgaa
     5821 gaaaaaaccg cacgttacgg tgcggcggtg ttggcggtgt tgggacaaaa tgccgtctga
     5881 aaaccgttac caagtttcag acggcattgc ctctatttaa aaattcct
//
LOCUS       DAWKOG010000129         5863 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.129, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000129 DAWKOG010000000
VERSION     DAWKOG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.129"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            41..454
                     /locus_tag="WJK66_001663"
     CDS             41..454
                     /locus_tag="WJK66_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ7579341.1"
                     /translation="MMPAYAYAALAFSAFTSATLLPGTSEAAFALFVHRFPEHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLAFAWLPVVGDALPLA
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
     gene            500..1129
                     /gene="nth"
                     /locus_tag="WJK66_001664"
     CDS             500..1129
                     /gene="nth"
                     /locus_tag="WJK66_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEZ7579342.1"
                     /translation="MNRQIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYKG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            1267..2766
                     /locus_tag="WJK66_001665"
     CDS             1267..2766
                     /locus_tag="WJK66_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEZ7579343.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSSNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSP
                     AERAGLRAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKDEEITIKVKLGN
                     AAEHIGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGGHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLIMRRGNTLFIALNLQ"
     gene            2968..4488
                     /locus_tag="WJK66_001666"
     CDS             2968..4488
                     /locus_tag="WJK66_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEZ7579344.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRTKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAVSDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRANA"
     gene            4545..5630
                     /gene="nagZ"
                     /locus_tag="WJK66_001667"
     CDS             4545..5630
                     /gene="nagZ"
                     /locus_tag="WJK66_001667"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEZ7579345.1"
                     /translation="MTVPCIPRGPVMADIAAFRLTEEEKQRLLDPAVGGVILFRRNFQ
                     NIAQLKELTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMNVLGQIWDKDGASA
                     AETAARQIGWVLATELSACGIDLSFTPVLDLDWGNCAVIGNRSFHRNPEAVARLALAL
                     QKGLEKGGMKSCGKHFPGHGFVEGDSHLVLPEDGRSLSELEAADLAPFRIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIVLVCNRPDLVDELRDDFRIPDNPALAQRWQYMANTLGSAAAQAVIQTAD
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            5711..5787
                     /locus_tag="WJK66_001668"
     tRNA            5711..5787
                     /locus_tag="WJK66_001668"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5745..5747,aa:Pro,seq:cgg)
BASE COUNT         1344 a         1783 c         1474 g         1262 t
ORIGIN      
        1 caatccgtcg ccgatttttg cctgtttgag actgtttgca atgatgcccg cctacgccta
       61 cgccgcgctc gctttttccg ccttcacttc cgccaccctc ctgcccggca catccgaagc
      121 cgcctttgcc ctgttcgtcc accgctttcc cgaacacgca tacggcgcgt tgctgtgcgc
      181 cggccttgcc aacggattgg gcagtatggt ttcctattgg atggggcgtt tgctgccctc
      241 ccgaaaaatg ccgtctgaaa aaacactgaa tctgatgcgg cgtttcggta tttggctgct
      301 tgcgtttgcc tggctgcccg tcgtcggcga cgcactgccg cttgccgccg gctggctgcg
      361 gttgaatccg tggacaagcg ggctgatgct ggttatcggc aaaacggcgc gttacgcctt
      421 cattctgtgg ggaatgcaat attacgccgc ctgaaccgtg cgttataatg ccgaacgcac
      481 aaaccgactg cacaaaaaaa tgaacagaca aatccgccaa gaaatcttcg agcgtttccg
      541 cgctgccaac ccccatccta ccacagaact caacttcaac tcccctttcg agcttttaat
      601 cgccgtttta ctgtcggcac aggcaaccga cgtcggcgta aacaaagcga cggcgaagct
      661 gtttccggtt gccgatacgc cgcaggcgat gctggatttg ggtttggacg gcgtgatgga
      721 atatacgaaa accatcgggt tgtataaaac caagtccaaa cacatcatgc agacctgccg
      781 catcctgttg gaaaaataca agggcgaagt gccggaagac cgcgaggctt tggaatcgtt
      841 gcctggcgtg gggcgcaaaa cggcaaacgt ggtattgaac acggcgttcg gacaccccgt
      901 tatggcggtc gatacgcata ttttccgcgt gtccaaccga accaaaatcg cccccgggaa
      961 agatgtgcgc gaagtcgaag acaaactgat gcgcttcatt cctaaagaat ttctgatgga
     1021 cgcgcaccac tggctgattt tgcacggacg ctacacctgc aaggctttaa agccgcaatg
     1081 ccaaacctgc atcatcaacg atttgtgcga atatcccgcc aaagcctgac cgccgccgtc
     1141 cttcaaaatg aaacgcttga aaattcggac ggacgttgta gaatttcaac ctatgccgtc
     1201 tgaagccgac aaaatgcgct tcagacggca ttaaagccgg taccggcatc cgacaaagga
     1261 caaaacgtgt tcaaaaaata ccaatacctc gctttggcag cactgtgtgc cgcctcgctg
     1321 gcaggctgcg acaaagccgg cagctttttc ggtgcggaca aaaaagaagc atctttcgta
     1381 gaacgcatcg aacacaccaa agacgacggc agcgtcagta tgctgctgcc cgactttgtc
     1441 caactggttc aaagcgaagg cccggcagtc gtcaatattc aggcagcccc cgccccgcgc
     1501 acccaaaacg gcagcagcaa tgccgaaacc gattccgacc cgcttgccga cagcgacccg
     1561 ttctacgaat ttttcaaacg cctcgtcccg aacatgcccg aaatccccca agaagaagca
     1621 gatgacggcg gattgaactt cggttcgggc ttcatcatca gcaaagacgg ctatattctg
     1681 accaatacgc acgtcgttac cggcatgggc agtatcaaag tcctgctcaa cgacaagcgc
     1741 gaatataccg ccaaactcat cggttcggat gtccaatccg atgtcgccct tctgaaaatc
     1801 gacgcaacgg aagagctgcc cgtcgtcaaa atcggcaatc ccaaagattt gaaaccgggc
     1861 gaatgggtcg ccgccatcgg cgcgcccttc ggcttcgaca acagcgtgac cgccggcatc
     1921 gtgtccgcca aaggcagaag cctgcccaac gaaagctaca cacccttcat ccaaaccgac
     1981 gttgccatca atccgggcaa ctccggcggc ccgctgttca acttaaaagg acaggtcgtc
     2041 ggcatcaact cgcaaatata cagccgcagc ggcggattca tgggcatttc cttcgccatc
     2101 ccgattgacg ttgccatgaa tgtcgccgaa cagctgaaaa acaccggcaa agtccaacgc
     2161 ggacaactgg gcgtgattat tcaagaagta tcctacggtt tggcacaatc gttcggtttg
     2221 gacaaagccg gcggcgcact gattgccaaa atcctgcccg gcagccccgc agaacgtgcc
     2281 ggcctgcggg cgggcgacat cgtcctcagc ctcgacggcg gagaaatacg ttcttccggc
     2341 gaccttcccg ttatggtcgg cgccattacg ccgggaaaag aagtcagcct cggcgtatgg
     2401 cgcaaagacg aagaaatcac aatcaaagtc aagctgggca acgccgccga gcatatcggc
     2461 gcatcatcca aaacagatga agccccctac accgaacagc aatccggtac gttctcggtc
     2521 gaatccgcag gcattaccct tcagacacat accgacagca gcggcggaca cctcgtcgtc
     2581 gtacgggttt ccgacgcggc agaacgcgca ggcttgaggc gcggcgacga aattcttgcc
     2641 gtcgggcaag tccccgtcaa tgacgaagcc ggtttccgca aagctatgga caaggcaggc
     2701 aaaaacgtcc ccctgctgat catgcgccgt ggcaacacgc tgtttatcgc attaaacctg
     2761 caataacaca cataatttaa ccaaaatatt ttttaaatga taaaatgccc gtcaataccg
     2821 attaacgggc attttacaaa aatcagttgg ttgattttaa acaaatattt tttacgacaa
     2881 aaaatatttc cgcccatttt gccaacaaaa cccaaaccgg aggcggacag atacgctccc
     2941 atccggacgc ttaacacccc cataaccatg cagctgatcg actattcaca ttcatttttc
     3001 tcggttgtgc cacccttttt ggcactggca cttgccgtca ttacccgccg cgtactgctg
     3061 tctttaggca tcggtattct ggtcggcgtt gcctttttgg tcggcggcaa ccccgtcgac
     3121 ggtctgacac acctgaaaga catggtcgtc ggcttggctt ggtcagacgg cgattggtcg
     3181 ctgggcaaac caaaaatctt ggttttcctg atacttttgg gtatttttac ttccctgctg
     3241 acctactccg gcagcaatca ggcgtttgcc gactgggcaa aacggcacat taaaaaccgg
     3301 cgcggcgcga aaatgctgac cgcctgcctc gtgttcgtaa cctttatcga cgactatttc
     3361 cacagtctcg ccgtcggtgc gattgcccgc cccgttaccg acaagtttaa agtttcccgc
     3421 accaaactcg cctacatcct cgactccact gccgcgccta tgtgcgtgct gatgcccgtt
     3481 tcaagctggg gcgcgtcgat tatcgccacg cttgccggac tgctcgttac ctacaaaatc
     3541 accgaataca cgccgatggg gacgtttgtc gccatgagcc tgatgaacta ttacgcactg
     3601 tttgccctga ttatggtgtt cgtcgtcgca tggttttcct tcgacatcgg ctcgatggca
     3661 cgtttcgaac aagccgcgtt gaacgaagcc cacgatgaaa ctgccgtttc agacgctacc
     3721 aaaggtcgtg tttacgcact gattattccc gttttggcct taatcgcctc aacggtttcc
     3781 gccatgatct acaccggcgc gcaggcaagc gaaaccttca gcattttggg ggcatttgaa
     3841 aacacggacg taaacacttc gctggtattc ggcggcactt gcggcgtcct tgccgtcgtt
     3901 ctctgcacgc tcggcacgat taaaaccgcc gactatccca aagccgtttg gcagggtgcg
     3961 aaatctatgt tcggcgcaat cgccatttta atcctcgctt ggctcatcag tacggttgtc
     4021 ggcgaaatgc acaccggcga ttacctctcc acactggttg cgggcaacat ccatcccggc
     4081 ttcctgcccg tcatcctctt cctgctcgcc agcgtgatgg cgtttgccac aggcacaagc
     4141 tgggggacgt tcggcattat gctgccaatt gccgccgcca tggcggtcaa agtcgaaccc
     4201 gcgctgatta tcccgtgtat gtccgcagta atggcggggg cggtatgcgg cgaccactgt
     4261 tcgcccatct ccgacacgac catcctgtcg tccaccggcg cgcgctgcaa ccacatcgac
     4321 cacgttacct cgcaactgcc ttatgccctg acggttgccg ccgccgccgc atcgggctac
     4381 ctcgcattgg gtctgacaaa atccgcgctg ttgggctttg gcacgacagg cattgtattg
     4441 gcggtgctga tttttctgtt gaaagataaa aaacgcgcca acgcctgacc cctttccctg
     4501 ttcagacggc atatgccgtc tgaagctttt tggagcaaac cgaaatgact gttccctgta
     4561 ttccgcgcgg ccccgtcatg gccgacattg ccgccttccg cctgaccgaa gaggaaaagc
     4621 aacgccttct cgatcccgcc gtcggcggag tcatcctgtt ccgccgcaac ttccaaaata
     4681 tcgcacagct caaagaactc accgccgaaa tcaaagccct gcgcacaccc gaactcatta
     4741 tcgccgtcga tcacgaaggc ggaagggtgc aacgtttcat cgaaggtttt acccgcctgc
     4801 ccgcaatgaa cgttttaggg caaatttggg acaaagacgg cgcgtccgcc gccgaaaccg
     4861 cagcccggca gataggctgg gttttggcaa ccgaactttc cgcctgcggc atcgacttgt
     4921 ccttcactcc cgtcttggat ttggactggg gaaactgcgc cgtcatcggc aaccgcagtt
     4981 tccaccgcaa tcccgaagcg gtcgcccgcc ttgccctcgc ccttcaaaaa ggtttggaaa
     5041 aaggcggtat gaaatcctgc ggtaaacatt ttcccggaca cggatttgtc gaaggagaca
     5101 gccatttggt gctacccgaa gacgggcgca gcctgtccga actcgaagcc gccgacctcg
     5161 cccccttccg cattatgagc cgcgaaggta tggcagcggt tatgcccgcc cacgtcgttt
     5221 atccgcaagt ggacacaaag cccgccgggt tttccgaaat ctggctcaaa caaattttgc
     5281 gccgcgacat cgggttcaaa ggcgtgattt tctcggacga tttaactatg gagggcgcgt
     5341 gcggggcagg cggcatcaaa gaacgcgccc gcatttcctt tgaggcaggt tgcgacatcg
     5401 tcctcgtctg caaccgtccc gaccttgtgg acgaactgcg cgatgatttc cgcattcctg
     5461 acaatcccgc tttggcgcaa cgttggcaat atatggccaa cacgctcggc agtgccgccg
     5521 cgcaagccgt tatacagacg gcggatttcc aagcggcgca agcctttgtt gccggccttg
     5581 cctcgccgca agacacggcg ggcggcgtaa aagtcggcga agccttttaa atccgcccga
     5641 cacgcggcaa aaaggcaaaa cggtataggc acgccgtatt ttttgcgcta taatcacgcc
     5701 ttacttaatt cggaatgtag cgcagcccgg tagcgcactt cgttcgggac gaaggggtcg
     5761 gaggttcgaa tcctctcatt ccgaccaaat ccaaaccgcc tcggtatcca ttgccgaggc
     5821 agtttttcct tttgacgtca acagaaaaat gccgtctgaa aca
//
LOCUS       DAWKOG010000130         5835 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.130, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000130 DAWKOG010000000
VERSION     DAWKOG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.130"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            311..2485
                     /locus_tag="WJK66_001669"
     CDS             311..2485
                     /locus_tag="WJK66_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ7579346.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKY
                     QDVSSEKPKAQKDQGGYGFAMRFKRRNLHPTAMPKENEVKLNESDWEATGLPGDPKNL
                     PERQKSVIEKVKTDDGSNIYSSPYLTQSNHQNGSAGNGANQPKNEVEDYKDFKYVYSG
                     WFYKHAAKEIDSDKKKFKLGDDGYIFYHGKEPSRQLPASGKVTYKGVWHFATDVKKSQ
                     KFRDIIQSSKGQGDRYSGFSGDEGEEYSNKNEATLKSGHEGYGFTSNLEVDFGNKKLT
                     GKLIRNNASLNNNTNNDKHTTQYYSLEAQVTGNRFSGTATATDKKENEAQHPFVSDSS
                     SLSGGFFGPQGEELGFRFLSNDQKVAVVGSAKTQDKAANGNTAAASGGTDAAASNGAA
                     GTSSENSKLTTVLDAVELTLNDKKIKNLDNFSNAAQLVVDGIMIPLLPETSESGSNQA
                     DKGKKGKNGKNGGTDFTYKTTYTPKSDEKDTQAQTGAGGAQAASGTESVNGGQAGTKT
                     YEVEVCCSNLNYLKYGMLTRKNSKSAMQAGGNSSQADAKTEQVEQSMFLQGERTDEKE
                     IPNEQNVVYRGSWYGHIANNTSTSWSGNASNATSGNRAEFTVNFGDKKITGTLTANDR
                     TQPTFTITANIKDNGFEGTAKTADLGFDLDQSNTTGTPKAYITDAKVQGGFYGPKAEE
                     LGGWFAYPGDKQTEKATDASGNGNSASSATVVFGAKRQKPVQ"
     gene            2571..5315
                     /gene="tbpA"
                     /locus_tag="WJK66_001670"
     CDS             2571..5315
                     /gene="tbpA"
                     /locus_tag="WJK66_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HEZ7579347.1"
                     /translation="MQQQHLFRLNILCLSLMTALPAYAENVQAGQSQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEDAGRGVQSFNRLVPVEDTNRYANFIVEEECENRNYETCKANP
                     KKEVVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILEHT
                     QQTFDTRDMTVPAFLTKAVFDENRKYGPIRGNGKYAGNHKYGGLFTNGENGALVGAEY
                     GTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADGS
                     DKNCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGSN
                     LRHQDYYFQNAKRAYSSKIPTQNGNKKISPNGRTNNPYWVSIGRGNVVTEQICRLGNN
                     TYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRTLS
                     WNAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRAGVQSKAVKIDPEKSFNKEAGIV
                     FKGDFGNLEASWFNNAYRDLIVRGYEAQIQNGKETAKGDPAYLNAQSARITGINVLGK
                     IDWNGVWDKLPEGWYSTFAYNRVHVRDIKKRADRTDIQSHLFDAIQPSRYVVGLGYDQ
                     PEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTIK
                     KHFTLRAGVYNLLNYRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEMKF
                     "
BASE COUNT         1691 a         1428 c         1465 g         1251 t
ORIGIN      
        1 tctgtcggtg cggaaactta tcgggtaaaa cggtttcttg agattttgcg tcttggattc
       61 ccacttttgt gggaatgacg cgattagagt ttcaaaattt attctaaata gctgaaactc
      121 aacgcactgg attcccgcct gcgtgggaat gacgggatat aggtttccgt gcggacgcgt
      181 tcggattcac gactgcgcgg gaatgacggg attttggtgt attccctaaa aaaataaaaa
      241 agtatttgca aatttgttaa aaataaataa aataataatc cttatcattc tttaattgaa
      301 ttgggtttat atgaacaatc cattggtgaa tcaggctgct atggtgctgc ctgtgttttt
      361 gttgagtgct tgtttgggcg gaggcggcag tttcgatctt gattctgtcg ataccgaagc
      421 cccgcgtccc gcgccaaagt atcaagatgt ttcttccgaa aagccgaaag cccaaaaaga
      481 ccaaggcgga tacggttttg caatgcgctt caagcggaga aatttgcatc ctacggcaat
      541 gcctaaagaa aatgaggtta aactgaacga gagtgattgg gaggcgacag gattgccagg
      601 cgatcccaaa aacttacccg agcgacagaa atcggttatt gaaaaagtaa aaacagacga
      661 cggcagcaat atttattctt ccccctatct cacgcaatca aaccatcaaa acggcagcgc
      721 cggcaacggt gcaaaccaac caaaaaatga agtagaagat tataaagatt tcaaatatgt
      781 ttattccggc tggttttata aacatgcagc gaaagaaata gattccgata aaaaaaaatt
      841 taagttaggc gacgacggtt atatctttta tcacggcaaa gaaccttccc gacaacttcc
      901 cgcttctgga aaagttacct ataaaggtgt gtggcatttt gcgaccgatg tcaaaaaatc
      961 ccaaaaattt cgcgatatta tccagtcttc aaaaggacaa ggcgacaggt atagcggatt
     1021 ttcgggcgat gaaggcgaag aatattccaa taaaaatgaa gcgactttaa aaagcggtca
     1081 tgaaggttat ggttttacct cgaatttaga agtggatttc ggcaataaga aattgacggg
     1141 taaattaata cgcaataatg cgagcctaaa taataatact aataatgaca agcacaccac
     1201 ccaatactac agccttgagg ctcaagtaac aggcaaccgc ttcagcggca cggcaacggc
     1261 aactgacaaa aaagagaatg aagcccaaca tccttttgtt tccgactcgt cttctttgag
     1321 cggcggcttt ttcggcccgc agggtgagga attgggtttc cgctttttga gcaacgatca
     1381 aaaagttgcc gttgtcggca gcgcgaaaac ccaagacaaa gccgcaaatg gcaatactgc
     1441 ggcggcttca ggcggcacag atgcggcagc atcaaacggt gcggcaggca cgtcgtctga
     1501 aaacagtaag ctgaccacgg ttttggatgc ggttgaattg acactaaacg acaagaaaat
     1561 caaaaatctc gacaacttca gcaatgccgc ccaactggtt gtcgacggca ttatgattcc
     1621 gctcctgccc gagacttccg aaagtgggag caatcaggca gataaaggta aaaaaggtaa
     1681 aaacggtaaa aacggcggaa cagactttac ctacaaaaca acctacacgc cgaaaagcga
     1741 tgaaaaagac acccaagccc aaacaggcgc gggcggcgcg caagctgctt cgggtactga
     1801 gagcgttaac ggcgggcagg caggaacgaa aacctatgaa gttgaagtct gctgttccaa
     1861 cctcaattat ctgaaatacg gaatgttgac gcgtaaaaac agcaagtccg cgatgcaggc
     1921 aggaggaaac agtagtcaag ctgatgctaa aacggaacaa gttgaacaaa gtatgttcct
     1981 ccaaggcgag cgcaccgatg aaaaagagat tccaaacgag caaaacgtcg tttatcgggg
     2041 gtcttggtac gggcatattg ccaacaacac aagcacaagc tggagcggca atgcttccaa
     2101 tgcaacgagt ggcaacaggg cggaatttac tgtgaatttt ggcgataaaa aaattaccgg
     2161 cacgttaacc gctaatgata ggactcagcc aacctttact atcacggcta atattaagga
     2221 caacggcttt gaaggtacgg caaaaactgc tgacttaggt tttgatctcg atcaaagcaa
     2281 taccaccggc acgcctaagg catatatcac agatgccaag gtgcagggcg gtttttacgg
     2341 gcccaaagcc gaagagttgg gcggatggtt tgcctatccg ggcgataaac aaacggaaaa
     2401 ggcaacagat gcatccggca atggaaattc agcaagcagt gcaactgtcg tattcggtgc
     2461 gaaacgccaa aagcctgtgc aataagcacg gttgccgaac aatcaagaat aaggcttcga
     2521 cggcatcgct ccttccgatg ccgtctgaaa gcgaagatta gggaaacact atgcaacagc
     2581 aacatttgtt ccgattaaat attttatgcc tgtctttaat gaccgcgctg cctgcttatg
     2641 cagaaaatgt gcaagccgga caatcacagg aaaaacagtt ggataccata caggtaaaag
     2701 ccaaaaaaca gaaaacccgc cgcgataacg aagtaaccgg tttgggcaaa ttggtcaaaa
     2761 ccgccgacac cctcagcaag gaacaggtac tcgatatccg cgacctgacg cgttacgacc
     2821 ccggcatcgc cgtggtcgaa caggggcgcg gcgcaagctc tggctactcg atacgcggta
     2881 tggacaaaaa ccgcgtttcc ttgacggtgg acggcttggc gcaaatacag tcctacaccg
     2941 cgcaggcggc attgggcggg acgaggacgg cgggcagcag cggcgcaatc aatgaaatcg
     3001 agtatgaaaa cgtcaaagct gtcgaaatca gcaaaggctc aaactcggtc gaacaaggca
     3061 gcggcgcatt ggcgggctcg gtcgcatttc aaaccaaaac cgccgacgat gttatcgggg
     3121 aaggcaggca gtggggcatt cagagtaaaa ccgcctattc cggcaaaaac cgggggctta
     3181 cccaatccat cgcgctggcg gggcgcatcg gcggtgcgga ggctttgctg atccacaccg
     3241 ggcggcgcgc gggggaaatc cgcgcacacg aagatgccgg acgcggcgtt cagagcttta
     3301 acaggctggt gccggttgag gataccaatc ggtacgcaaa tttcatcgtt gaggaagaat
     3361 gcgaaaacag aaattacgaa acgtgtaaag cgaatccgaa aaaagaggtt gtcggcaaag
     3421 acgaacggca aacggtttcc acccgagact acacgggtcc caaccgcttc ctcgccgatc
     3481 cgctttcata cgaaagccga tcgtggttgt tccgcccggg ttttcgtttt gaaaacaaac
     3541 ggcactacat cggcggcata ctcgaacaca cgcaacaaac tttcgacacg cgcgatatga
     3601 cggttccggc attcctgacc aaggcggttt ttgatgaaaa tagaaaatac ggaccgataa
     3661 gaggcaacgg caaatatgcg ggcaaccaca aatacggcgg actgtttacc aacggcgaaa
     3721 acggtgcgct ggtgggcgcg gaatacggta caggcgtgtt ttacgacgag acgcacacca
     3781 aaagccgcta cggtttggaa tatgtctata ccaatgccga taaagacact tgggcggatt
     3841 atgcccgcct ctcttatgac cggcagggta tcggtttgga caatcatttt cagcagacgc
     3901 actgttctgc cgacggttcg gacaaaaatt gccgtccgag tgccgacaag ccgttttcct
     3961 attacaaatc cgaccgcgtg atttacgggg aaagccacag gctcttgcag gcggcattca
     4021 aaaaatcctt cgataccgcc aaaatccgcc acaacctgag cgtgaatctc gggtttgacc
     4081 gctttggctc taatctccgc catcaggatt attattttca aaacgccaaa cgcgcctatt
     4141 cgtcgaaaat acccactcaa aacggcaata aaaaaatcag ccccaacggc agaacaaaca
     4201 atccctattg ggtcagcata ggcaggggaa atgtggttac ggagcaaatc tgccgcttgg
     4261 gcaacaatac ttatacggac tgcacgccgc gcagcatcaa cggcaaaagc tattacgcgg
     4321 cagttcggga caatgtccgt ttgggcaggt gggcggatgt cggcgcgggg ttgcgctacg
     4381 actaccgcag cacgcattcg gacgacggca gcgtttccac cggcacgcac cgcaccctgt
     4441 cctggaacgc cggcatcgtc cttaaacctg ccgactggct ggatttgact taccgcactt
     4501 caaccggctt ccgcctgccc tcgtttgcgg aaatgtacgg ctggcgggcg ggtgttcaaa
     4561 gcaaggcggt caaaatcgat ccggaaaaat cgttcaacaa agaagccggc atcgtgttta
     4621 aaggcgattt cggcaacttg gaggcaagtt ggttcaacaa tgcctaccgc gatttgattg
     4681 tccggggtta tgaagcgcaa attcaaaacg gcaaagaaac agccaaaggc gacccggctt
     4741 acctcaatgc ccaaagcgcg cggattaccg gcatcaatgt tttgggcaaa atcgattgga
     4801 acggcgtatg ggataaattg cccgaaggtt ggtattctac atttgcctat aatcgtgtcc
     4861 atgtccgcga catcaaaaaa cgcgcagacc gcaccgatat tcaatcacat ctgtttgatg
     4921 ccatccaacc ctcgcgctat gtcgtcggct tgggctatga ccaaccggaa ggcaaatggg
     4981 gtgtgaacgg tatgctgact tattccaaag ccaaggaaat cacagagttg ttgggcagcc
     5041 gggctttgct caacggcaac agccgcaata caaaagccac cgcacgccgt acccgccctt
     5101 ggtatattgt ggatgtgtcc ggttattaca cgattaaaaa acacttcacc ctccgtgcgg
     5161 gcgtgtacaa cctcctcaac taccgctatg ttacttggga aaatgtgcgg caaactgccg
     5221 gcggcgcagt caatcaacac aaaaatgtcg gcgtttacaa ccgatatgcc gcccccggcc
     5281 gaaactacac atttagcttg gaaatgaagt tttaaacgtc caaacgccgc aaatgccgtc
     5341 tgaaaggctt cagacggcat tttttacaca accccaccgt ttcccatcat ccccgataca
     5401 ccgtaatcct gaaatccgtc attcccgagc aggcgggaat ccagtccgtt cggtttcggt
     5461 ttttttgagg tttcgggtaa ctctaaaccg tcattttctc gaaagcggaa aatcaaaaac
     5521 agaaatctaa aatcccgtca ttcccgcgaa agtgggaatc tagacctgtc ggtgcggaaa
     5581 cttatcggat aaaacggttt ctttagattt tacgttctag attcccgctt tcgcgggaat
     5641 gacgggatgc aggtttctgt tcggatgtat tcggattccc tcctgcgcgg gaatgatggg
     5701 gtttttaaat gtccgaatgc cgcaaatgcc gtctgaaagg cttcagacgg cattttttac
     5761 acaattccca ccgtttccca tccttctcga tacactgtaa tctcaaatcc cgtcattccc
     5821 gcgcaggcgg gaatc
//
LOCUS       DAWKOG010000131         5819 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.131, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000131 DAWKOG010000000
VERSION     DAWKOG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5819
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.131"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            334..5769
                     /gene="iga"
                     /locus_tag="WJK66_001671"
     CDS             334..5769
                     /gene="iga"
                     /locus_tag="WJK66_001671"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HEZ7579348.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNRRTLTVIDPQYAVSVKHV
                     KGDEISYYGHHNGHLDVSNDENEYRSVAQNDYEPNKNWHHGNQGRLEDYNMARLNKFV
                     TEVAPIAPTSAGGGVETYKDKNRFSEFVRVGAGTQFEYNSRYNMTELSRAYRYAIAGT
                     PYQDVNVTSNLNQEGLIGFGDNSKHHSPEKLKEVLSQNALTNYAVLGDSGSPLFAYDK
                     QEKRWVFLGAYDYWAGYQKNSWQEWNIYKKEFADEIKQRDNAGTIKGYGEHHWKTTGT
                     NSHIGSTAVRLAGNERGANNGQNVTFENNGTLVLDQNINQGAGGLFFKGDYTVKGINN
                     DITWLGAGIDVADGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQGQLKVGDGTVIL
                     NQKADSNQKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRLDANGNDLTFEH
                     IRNVDEGARIVNHNTGHTSTITLTGKSLITNPNSLSVHSIQNDYDEDDYSYYYRPRRP
                     IPQGKDLYYKNYRYYALKSGGSVNAPMPENGQTENNDWILMGSTQEEAKKNAMNHKNN
                     QRISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGTNLNGKISVTQGNVLLSG
                     RPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKATEIAVNQSASFSSGRNVS
                     DITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTGNLSDKALNSFGATQINGNV
                     NLSQNAALTLGKAALWGQIQGQGNSRVSLNQHSKWHLTGDSQVHNLSLADSHIHLNNA
                     SDAQSANKYHTLKINHLSGNGHFHYLTHLAKNLGDKVLVKESASGHYQLHVQDKTGEP
                     NQEGLNLFDASSVRDRSHLSVSLANNHVDLGALRYTIKTENGITRLYNPYAENRRRVK
                     PAPSPATNTASQAQTDSAQIAKPQNIVVAPPSPQANQAEEAKRQQAKAEQVKRQQAEA
                     ERKSAELAKQKAEAEREARELATRQKAEQERSSAELARRHEKEREAAELSAKQKVEAE
                     REAQALAVRRKAEAEGAKRQAAELARRHEKEREAAELSAKQRVGEEERRQTAQSQPQR
                     RKRRAAPQDYMAASQDRPKRRGHRSVQQNNVEIAQAQAELARRQQEERKAAELLAKQR
                     AEAEREAQALAARRKAEAEEAKRQAAELAHRQEAERKAAELSANQKAAAEAQALAARQ
                     QKALARQQEEARKAAELAVKQKAETERKTAELAKQRAAAEAAKRQQEARQTAELARRQ
                     EAERQAAELSAKQKAETDREAAESAKRKAEEEEHRQAAQSQPQRRKRRAAPQDYMAAS
                     QNRPKRRGRRSTLPAPPSPSFDSSAYAAPRALHNPDWYENDYEEIPLDALEDENVSES
                     VDTSDKQPQDNTELHEKYENDYEEIPLDALEDEDVSESVDTSDKQPQDNTELHEKVET
                     VSLQPRAAQPRAQAATQPQAQAAAQADAVSTNTNSALSDAMASTQSILLDTGASLTRH
                     IAQKSRADAEKNSVWMSNIGYGRDYASAQYRRFSSKRTQTQIGIDRSLSENMQIGGVL
                     TYSDSQHTFDQASGKNTFVQANLYGKYYLNDAWYMAGDIGAGSLRSRLQTQQKANFNR
                     TSIQTGLTLGNTLKINQFEIVPSAGIRYSRLSSADYKLGNDSVKVSSMSVKTLTAGLD
                     FAYRFKVGNLTVKPLLSAAYFANYGKGGVNVGGNSFVYKADNQQQYSAGAALLYRNVT
                     LNVNGSITKGKQLEKQKSGQIKIQIRF"
BASE COUNT         1959 a         1396 c         1301 g         1163 t
ORIGIN      
        1 cgtcattccc acgaaccccc atctcgtcat taccacgaaa gcgggaatct aggacgcagg
       61 gttaagaaaa cctacatccc gtcattcccg cacaagcggg aatccaaaaa caaaaagtcg
      121 cagaaattta acggaagaac cgaaccccga aaatcggctt cccgccttcg cttgaataac
      181 agaacaaagg aagcggaaat aaaaacatcg ggattctttt tttaaattga caataaaaca
      241 ccaaaattaa tgatttacac aacaaaaaat agtcaacacc gccaaaccgg cgtaaaatac
      301 aagacattat cacccttaaa acggtaaaac cttatgaaaa ccaaacgttt taaaattaac
      361 gccatatcct tatccatctt tcttgcctat gcccttacgc catactcaga agcggcattg
      421 gtcagagacg atgtcgatta tcaaatattc cgtgactttg cagaaaacaa aggcaaattt
      481 tttgtcggcg caaccgattt atcagtgaaa aacaaacaag gtcaaaacat cggcaacgca
      541 ttatccaacg taccgatgat tgattttagc gttgcagatg tcaacagacg tacattaacc
      601 gtcatcgatc cccagtatgc cgtcagcgtc aaacatgtaa aaggagacga aatctcttat
      661 tacgggcatc acaatgggca cttagatgtt agcaatgatg aaaacgaata tcgttcagtt
      721 gcacaaaatg actacgaacc aaataaaaat tggcatcacg gtaatcaagg tcgtcttgaa
      781 gactacaaca tggcacgcct taataaattc gtaacagaag tcgcacctat tgcaccaacc
      841 agtgcaggcg gcggcgttga aacctataaa gataaaaacc gtttttctga gtttgtgcga
      901 gttggagcag gtacgcaatt tgaatataac agccgctaca atatgacaga gctttcacga
      961 gcttatcgtt atgccattgc aggtacgcct tatcaagacg tgaatgtcac atcgaatcta
     1021 aatcaagaag gcttgattgg cttcggtgat aattcaaaac atcattcccc agaaaaactc
     1081 aaagaagttt tatctcaaaa cgcattgact aactatgctg tgttaggcga tagcggttca
     1141 ccattatttg cttatgataa acaagaaaaa cgctgggtct ttttaggtgc ttacgattat
     1201 tgggcaggct atcaaaaaaa ctcttggcaa gaatggaata tctataaaaa agaatttgca
     1261 gatgaaatca aacaacgcga taacgccggc accatcaaag gttatggcga acatcattgg
     1321 aaaaccacgg gcacaaacag ccatatcggt tcgacagcgg taaggcttgc cggcaatgaa
     1381 aggggtgcaa acaacggaca gaatgttacc tttgaaaaca acggcacttt ggtattggat
     1441 caaaacatca accaaggcgc gggcggtctg tttttcaaag gcgattacac agtcaaaggt
     1501 ataaataatg acatcacttg gctaggtgcg gggattgatg ttgccgacgg caaaaaagtc
     1561 gtttggcaag tcaaaaatcc gaatggcgac agattggcga aaatcggcaa aggcacatta
     1621 gaaataaacg gcacaggcgt taaccaaggg caattaaaag tcggcgacgg tacggttatt
     1681 ctgaatcaaa aagccgattc aaaccaaaaa gtccaggctt tctcccaagt cggcattgtc
     1741 agcggacgcg gtacattggt attaaatagt tcaaatcaga ttaatcccga taacctatat
     1801 ttcggtttcc gtggcggtcg tttggatgcc aatggcaatg acttgacttt tgaacacatc
     1861 cgcaacgtgg atgaaggcgc gcgcattgtc aaccacaaca caggccacac ctccacaatc
     1921 acgctaacgg gtaaatcttt aattaccaat ccgaacagcc tttcggtaca tagtatacaa
     1981 aatgactatg atgaagatga ttatagttat tattaccgac caaggcgacc catcccacaa
     2041 ggcaaagatc tttattacaa aaactatcgt tattacgccc taaaatccgg cggcagcgtg
     2101 aacgcaccga tgcccgaaaa cggacaaacg gaaaataacg actggatttt aatgggcagc
     2161 acacaagaag aagccaagaa aaacgcgatg aaccacaaaa acaatcagcg tattagcggt
     2221 tttagcggtt tctttggcga agaaaacgga aaaggacata acggtgcatt aaaccttaat
     2281 ttcaacggca aaagcgcgca aaaccgtttc ttgttaacag gtggcactaa tttaaacgga
     2341 aaaataagcg taactcaagg caatgtcttg ttatcaggtc gtccaacacc acacgcaaga
     2401 gattttgtga acaaatcttc agcccgaaaa gacgcacatt tctccaaaaa caatgaagtc
     2461 gtatttgaag acgactggat aaaccgcaca ttcaaagcca cagaaattgc ggttaatcaa
     2521 tccgcatcat tctcttccgg cagaaatgta tccgacatca ccgccaacat caccgcaaca
     2581 gacaacgcca aagtaaattt gggttacaaa aacggcgatg aggtttgcgt gcgctcggac
     2641 tataccggtt atgttacctg caacacaggc aacttatccg ataaggcttt aaacagcttt
     2701 ggagcgacac agattaacgg caatgtgaat ttgagtcaga atgcggcatt gactttgggc
     2761 aaggctgcat tatgggggca aattcaaggg caaggaaaca gccgtgtcag cctaaatcaa
     2821 catagcaaat ggcatttgac cggcgacagc caagtacaca atctgtcatt ggcggatagc
     2881 catattcatt tgaacaacgc ttccgacgcg caaagtgcaa ataaatacca cacgctcaaa
     2941 atcaatcatt tatccggtaa cgggcatttt cattatctga cgcacttggc aaaaaatctt
     3001 ggggataaag tgcttgtgaa ggaatccgca tccggtcatt atcagctcca tgttcaagat
     3061 aaaacaggcg aacccaatca ggaagggctg aatctctttg atgcatcatc cgtacgagac
     3121 cgatcgcacc tttccgtttc cttggcgaat aaccatgtag atttaggcgc attgcgttat
     3181 acaatcaaaa cagaaaatgg cattacccga ttgtacaatc cttatgccga gaaccgccgc
     3241 cgagtcaaac cggccccgtc tcctgccaca aacacggctt ctcaagcaca aacggacagt
     3301 gcacaaattg ccaagcctca aaatatcgtc gtcgcaccgc ctagcccgca ggcaaatcaa
     3361 gccgaagaag caaaacgcca acaagcaaaa gcggagcaag tgaagcgtca gcaagcagaa
     3421 gcagagcgta aatcggcaga gttagcaaaa caaaaagcag aagccgaacg cgaagcaaga
     3481 gagctggcga cccgccaaaa agcagaacaa gaacgcagca gcgccgaact tgcacgtcga
     3541 catgaaaaag aacgcgaagc ggcagagttg tcggcaaaac aaaaagtcga agccgaacgt
     3601 gaggctcaag cattggcggt acgccgaaaa gcagaagccg aaggggcaaa acgccaagct
     3661 gcagagcttg cacgccggca tgaaaaagaa cgcgaagcgg cagagttgtc ggcaaaacaa
     3721 agggtcgggg aagaggaacg ccgtcaaaca gcccaatccc agccgcaacg ccgcaaacgc
     3781 cgtgccgcac cgcaggatta tatggcagct tcccaagacc gtcctaaacg ccgcggacac
     3841 cgttcggtac agcaaaacaa tgtggaaatt gcccaagccc aagcagaact tgcgcgccga
     3901 cagcaggaag aacgcaaagc ggcagagttg ttggcaaaac aaagggcaga agccgaacgt
     3961 gaggctcaag cattggctgc gcgccgaaaa gcagaagccg aagaggcaaa acgccaagct
     4021 gccgaacttg cacatcgcca ggaagcagaa cgcaaagcgg cagaattgtc ggcaaaccaa
     4081 aaagccgcag ctgaagccca agcattggcg gcacgccaac aaaaagccct tgcgcgtcaa
     4141 caggaagaag cacgaaaagc tgcagagtta gccgtcaaac aaaaagcgga aaccgaacgc
     4201 aaaaccgccg aacttgccaa gcaaagggca gcagccgaag cagcaaaacg ccagcaagaa
     4261 gctcgccaga ccgccgaact tgcacgtcgt caagaagcgg aacgccaagc agcagagttg
     4321 tcggcaaaac aaaaagccga aacagatcga gaagcggcag aatcggcgaa acgaaaagct
     4381 gaggaagaag agcaccgtca agcagcccaa tcccagccgc aacgccgaaa acgccgtgcc
     4441 gcacctcagg attatatggc agcttcccaa aatcgtccga aacgtcgcgg acgcagatct
     4501 actctgccgg caccgccctc gccatcattt gattcgtcag cttacgcagc acccagggcc
     4561 ttgcataatc cggactggta tgaaaatgac tatgaagaaa tccccttgga tgcgttggaa
     4621 gatgaaaatg tatctgaatc agttgacaca tcagacaaac agcctcaaga caatacggaa
     4681 cttcatgaaa aatatgaaaa tgattatgaa gaaatcccct tggatgcgtt ggaagacgaa
     4741 gatgtatccg aatcggttga cacatcagac aaacagcctc aagacaatac ggaacttcat
     4801 gaaaaagttg agacagtgag tttgcaacca agagccgcgc agccgcgagc ccaagccgcc
     4861 actcagccgc aagcccaagc cgccgcgcaa gctgatgcag tcagcaccaa taccaactcg
     4921 gctttatctg acgcaatggc aagcacgcaa tctatcttgt tggatacagg tgcttcatta
     4981 acacggcaca ttgcacaaaa atcacgcgct gatgccgaaa aaaacagcgt ttggatgtcg
     5041 aacatcggtt atggccgtga ttatgcttcc gcacaatatc gccggtttag ttcgaaacgc
     5101 acgcaaacac aaatcggcat tgaccgcagc ttgtccgaaa atatgcagat aggcggcgta
     5161 ttgacttact ctgacagtca gcatactttt gatcaggcga gcggcaaaaa tacttttgtg
     5221 caagccaacc tttatggtaa gtattattta aatgatgctt ggtatatggc cggcgatatt
     5281 ggtgcaggca gcttgagaag ccggttacaa acgcagcaaa aagcaaactt taaccgaaca
     5341 agcatccaaa ccggccttac tttgggcaat acactgaaaa tcaatcaatt cgagattgtc
     5401 cccagtgcgg ggatccgtta cagccgcctg tcatctgcag attacaagtt gggtaacgac
     5461 agtgttaaag taagttctat gtcagtgaaa acactgacgg ccggactgga ttttgcttat
     5521 cggtttaaag tcggcaacct taccgtaaaa cccttgttat ctgcagctta ctttgccaat
     5581 tatggcaaag gcggcgtgaa tgtgggcggt aattccttcg tctataaagc agataatcaa
     5641 cagcaatatt cagcaggtgc cgcgttactg taccgtaatg ttacattaaa cgtaaatggc
     5701 agtattacaa aaggaaaaca attggaaaaa caaaaatccg gacaaattaa aatacagatt
     5761 cgtttctaaa atactaaatt catagcaaaa taaaatgccg tctgaactca ggcttcaga
//
LOCUS       DAWKOG010000132         5801 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.132, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000132 DAWKOG010000000
VERSION     DAWKOG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.132"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            132..566
                     /locus_tag="WJK66_001672"
     CDS             132..566
                     /locus_tag="WJK66_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEZ7579349.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPLNDFQPNCD
                     IRRLGLTQGQHNELRKIRAAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(579..1442)
                     /locus_tag="WJK66_001673"
     CDS             complement(579..1442)
                     /locus_tag="WJK66_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ7579350.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHTFPLARLAGVGRQTELLVRNTEQFIAGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYFDENAGTTGMRGEIHQKEAVEEKISLSDRF
                     GLWLSFYPFDQNDYLAAVQNWLEDFGVPYDETAQMAALQWAQTRGSRSGRSAWQFACD
                     WAGRLPKQRAL"
     gene            complement(1450..2067)
                     /locus_tag="WJK66_001674"
     CDS             complement(1450..2067)
                     /locus_tag="WJK66_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEZ7579351.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFQTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGYNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDSRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(2230..2844)
                     /locus_tag="WJK66_001675"
     CDS             complement(2230..2844)
                     /locus_tag="WJK66_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEZ7579352.1"
                     /translation="MKTSNFSKRTSAMKTFALILADGQASRMGGEDKGLALLGGKALI
                     DHVIDRVRPQVSHIAISTNRNLEEYARRSPHIFPDARQWQHFGPLSALCTAANDLQLA
                     TADWLLVVPCDMPYLPDDLVARFETVSKRTPLCNAYYVETPITMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARPVRFEFDGHFADLNTQTDLQEG"
     gene            3050..4822
                     /locus_tag="WJK66_001676"
     CDS             3050..4822
                     /locus_tag="WJK66_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HEZ7579353.1"
                     /translation="MILPARFSDGISLSLRLKLLTGVWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRAQIDNQVAEFEKSLNRISQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGGSDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFPNGISLDEDDRILLQTLGRQLGVSLAGTKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAAENIGFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGITVETAWENGSFLPPQEAQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIRAV
                     LKIYSQPQQGTTVSLAGVSEESLK"
     gene            4819..5475
                     /locus_tag="WJK66_001677"
     CDS             4819..5475
                     /locus_tag="WJK66_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ7579354.1"
                     /translation="MTIKIILIDDHTLFRSGIKSLLSRQHDFEVIGEAADGLSGIKMI
                     NRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDNDDLTECMRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGRSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVLE
                     "
BASE COUNT         1429 a         1629 c         1348 g         1395 t
ORIGIN      
        1 gttccgattc cgccgttatg tctgcctgcc ggacatacgt acgcattatt aacaaaagtt
       61 aaccgcgata ataccatctt tcacacgtca atctagtata tttcctaaaa tttccaacaa
      121 gaggaaaagc cgtgccactg cctgctccct gccgttttgc caaacctgcc gcctcttttt
      181 taagtatggc tttgctttcc tgccagcttt cccacgccgc cacggcttat atccccctga
      241 acgattttca accgaactgc gacatacgcc ggctcggact gacacagggt cagcacaatg
      301 aactgcgtaa aatccgcgcc gccttcaaaa tggcgggcga cagggcgcgt ttgaaggtta
      361 tgcattccga acacagccgc cgccggtctg tcgtcgaaat catttcctcg gatgttttta
      421 atcggaacga ggcgcgcgat tatgtcgaaa gccgctattt gtccggtatg gattttgcgg
      481 tggacgaatt ggaaatccaa caccggttct tccatatcct cacaccgcaa cagcagcaaa
      541 tgtggctttc ttcctgcctc aaataatccc cgaaacgctc acaacgcccg ttgtttcggc
      601 agcctgcccg cccagtcgca ggcaaactgc cacgcggaac gtcccgaacg gctgccccgc
      661 gtctgcgccc actgcaatgc cgccatctgt gcggtttcat catacggcac gccgaaatct
      721 tccagccaat tttgcaccgc cgccagataa tcgttttgat cgaacggata aaaactgagc
      781 cacaatccga atcggtcgga cagggatatt ttttcttcca ccgcttcttt ctgatggatt
      841 tccccccgca tccccgtcgt accggcattc tcgtcaaaat attcgggcat caggtgccgt
      901 ctgttggaag tcgcgtaaac caaaacgttg gcgcaacgtt gagacagacc gccgtctaac
      961 gcggttttca atgccttata ggtttcatcg ccgctttcaa acgacaaatc gtcgcaaaat
     1021 acgataaatt tttccggacg ttctttcaaa agcgtcaaca ggtaaggcag gccgattaaa
     1081 tcgcttttat cgacttcgat caggcgcaat cccttatccg catattcgtg cagcagggct
     1141 ttgaccagcg aggatttgcc tgttccgcgc gcgccgctca tcaatacgtt attcgcgggt
     1201 ctgccggcaa tgaattgttc ggtattacgc accagcaatt cggtttgcct gccaactccc
     1261 gccagccttg ccaaggggaa ggtgtgcgga tcgggcaagt gttccaaaaa accttttttg
     1321 cccgcactct gccagcggaa ggcaagcgcg ttccaatccg tatgcccggg ttcgggcgga
     1381 agcacggcat ccaaacgccg caaaacggca taggctttat cgagaaattc gttcaattcc
     1441 atctctacct cactttgcat atctttgcgc catcagccgt tcgacggtat cgacgattgc
     1501 ctgcgtattc ggatcgattt cgatgttgat tctgtcgccg acctttctgc tgccgaacag
     1561 cgtccgttcc aaagtttcgg gaatcaaatg gacattgaaa cggctgtctt cgactttgcc
     1621 tatggtcagg ctgcaaccgt ccaagccgac gaaccctttg gtcaggatat agggtttgag
     1681 ttcgtgcggg agcgaaaacc aaaccgtgcg gttgtacccg tcccgttcga tttcgacaat
     1741 aggcacggtt gccataatgt gtccgctcat gacgtgtccg ccgatttcgt ctccgaaacg
     1801 cgccgcccgt tcgatgttga cgcaatcgcc ttccttcagc agccctaaat tggtttttgc
     1861 caaagtttcc gccattaaat cgaaactgac gcggtttcct tcgatttcgg taatcgtcag
     1921 gcagcagccg ttgttggcga ccgatgcgcc gcgttgcaga ttgtccgccg cctcttgcgg
     1981 aagctcgacg acataagtct gaaatgcctc cgacgggcgg tggattgccg tcagttttcc
     2041 caatccttga acaatgcctg taaacataat cctgtttccc tgtgtcggta aaaatggtgc
     2101 aaattgtagc atctccccgc gaaaaatgcc gtctgaaatg ccttcagaca gcattatgcc
     2161 tccgattcgg gcaaaaaccg cccggtacgg cttgaccttt cctttccacg ccggtcggcg
     2221 gtcttaccct tatccctcct gcaaatcagt ttgcgtgttc aagtcggcaa aatgcccgtc
     2281 aaactcgaat ctgaccggtc gcgccctttg ctgctgcaac cagcttctta atgttttcat
     2341 acccgaaaac agatagggaa tcgcgctttg cagaatctgc gggcggatat acataatgtt
     2401 gtagtgcatc gttatcggcg tttccacata atacgcattg cacaacggtg tgcgtttcga
     2461 cacggtttca aacctcgcca ccaaatcgtc cggcagatac ggcatatcgc acggcacaac
     2521 caaaagccag tcggcagtcg ccaactgcaa atcgttggct gcggtacaca atgccgaaag
     2581 cgggccgaaa tgctgccact gccgtgcgtc cggaaaaata tgcggacttc ttcgagcata
     2641 ttcttccaaa ttccggttgg tgctgatggc gatatggctg acctgcggcc tgaccctgtc
     2701 gatgacatga tctatcagtg ccttaccccc caaaagagca agccccttgt cctcgcctcc
     2761 catacggctc gcctgaccgt cggccagtat cagggcaaaa gttttcattg cggatgttct
     2821 cttggaaaag ttcgaggttt tcatgattgc agtctgccgt tcccaatgac tcaaaatgcc
     2881 gtctgaagca gacggcaaat aaattcatat tatctgaatt ttatcataac atgatttaac
     2941 actgaagcgg tgcggattaa gttttcctta accaattctt tctgagcagt tgtatctaat
     3001 tccaaagaat gatattgttt gcattatttg gaacaatttt tcgccgagca tgatactgcc
     3061 agcccgtttt tcagacggca tcagcctttc cctacgcctg aaactcctga ccggagtgtg
     3121 ggtcgggttg gcggcattgt ctgtcgtttt gacactgctg ctctctttgc gtctggaaaa
     3181 cgcggcctcc gtcatcgaag aggcgggcaa cttgagaatg caggcatacc gtctggcata
     3241 catggcgggt gaaggctcgc cccgtgcgca aattgacaat caggttgccg aatttgaaaa
     3301 aagcctgaat cgtatttcac aaagtgatgc catccatccg ctgattcctt cggacacccc
     3361 tcttgcttat gatttgatac aatccatgct gattatagat tggcaggcac acatcctccc
     3421 cccgctccag tcctaccggc gaccgactca ggtcgatctc taccgctttg ccggaaacat
     3481 cgaactgttt ttgcaggcat tggaaaatgc caacgaaaaa aacacatggt ggctcaggcg
     3541 ttttcaatgg gcaattatgt tgatgacgct ggtgtcgtct gtactgatgc tgttttggca
     3601 ccagatttgg gttatccggc cgctgcaggc gttaagggaa ggtgcggaac gcatcggacg
     3661 gaggtgtttc gatattccgg ttcccgaagg cggtacgccg gaattcaaac aggtcgggcg
     3721 ttgtttcaat caaatgggcg gcaggttgaa aattttatat gatgatttgg aaggacaagt
     3781 cgccgagcag acacgcagtc tcgaaaaaca aaatcaaaac ctgaccctgc tgtaccaaac
     3841 tacacgggac ctgcaccaat cctacatacc gcaacaggct gcagaacatt ttctaaaccg
     3901 tatcctgccc gccgtaggag cagattccgg cagagtttgt ttggacggcg gatccgatgt
     3961 ttatgtttcc attcatcatg cggattgcgg cacagcagct tcggatttgg ggaagtacca
     4021 tgaggaaatc ttccccattg agtaccagaa cgaaacattg ggcaggctgt tgctcagctt
     4081 tccaaacggc atttctcttg atgaagacga ccgcatcctg cttcaaacac taggcaggca
     4141 attgggcgta tcgcttgccg gcacaaaaca ggaggaagaa aaacgcctgc ttgcagtatt
     4201 gcaggaacgc aacctgattg cgcaaggatt acatgacagc atcgcacaag cattaacgtt
     4261 cctaaaccta caggtacaga tgctggaaac cgcctttgcc gaaaacaaac gggaggaagc
     4321 cgcagaaaac atcggcttca tcaaaacagg cgtgcaggaa tgttatgaag atgtccgcga
     4381 actgctgctc aacttccgta ccaaaatcag caataaagaa tttcccgaag ccgttgccga
     4441 cctattcgcc cgctttacgc aacaaaccgg gataacggtc gaaaccgcct gggaaaacgg
     4501 ttcgttcctg ccgcctcagg aagcgcagct ccaaatgatt tttatcctgc aggaaagcct
     4561 gtccaacatc cgcaaacacg cccgcgccac ccatgtaaaa ttcacccttt ccgaacacgg
     4621 cggacgcttt accatgacca tccaagacaa cggacaaggt ttcgacacgg agaaaatagg
     4681 agaacctacg ggcagccatg tcggactgca tatcatgcag gagcgtgcca aacgtatccg
     4741 cgccgtattg aaaatctatt cccaacccca acagggaacc accgtctcat tggcgggtgt
     4801 atctgaagaa agcttgaaat gaccattaaa attatcctga tagacgacca taccctcttc
     4861 cgcagcggca ttaaatccct tttgtcgcgc caacatgatt ttgaggtcat cggcgaagcc
     4921 gcagacggcc tctcgggcat taaaatgatc aaccggctcc aacccgatgt cgtcctgctt
     4981 gaccttgata tgcccggtat gaacggacgc gaagcactct cccaaatcat cagcatcaat
     5041 ccgcagcagg cagtgattat gctgaccgtt tccgaagaca acgacgactt gaccgaatgt
     5101 atgcgcatcg gcgcgcgcgg ctacctgctg aaaaacatca acgccgactt tctgctcgaa
     5161 agcatacgca aagccgccga aggcgataat gtattctcgc ccgagatgac cgccaaactc
     5221 gttaaaagcc tgatttcccc ccaacctgcc caagggacac aggcactctc ctcacttacc
     5281 cctcgcgaac tggaaatctt gggctatctc gccgcaggac gcagcaacaa aatcatcgcc
     5341 cgccacctcg atcttgccga atccaccgtc aaagtccacg ttcaaaacct gctccgcaaa
     5401 ctcaacctca gcagccgggt gcaggccgct gtttacgcca tccggcacaa cgtcccccaa
     5461 cccgtgctgg aataaggcgg gttcagacgg catattaggg ggtttaatcc ccgtacggtc
     5521 attcggataa cagaccaagc atgtaagctt atgcccccat aagtacgctt ggcatagcag
     5581 taatattgtt cggtttagtg ttttccgttt gcccctatct gatactgcaa tatcagctat
     5641 gccgtctgaa aacgcatcat catgatattt tcagacggca taataaaaag cggaaatact
     5701 aatgcagggt aaaatgttcc attccgaatc ccataaatat acaatggctt atatcgttta
     5761 agcatgtgta acccaccctc atatcaatca atatatagtg g
//
LOCUS       DAWKOG010000133         5659 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.133, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000133 DAWKOG010000000
VERSION     DAWKOG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.133"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            97..813
                     /locus_tag="WJK66_001678"
     CDS             97..813
                     /locus_tag="WJK66_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ7579355.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILADLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            962..1864
                     /locus_tag="WJK66_001679"
     CDS             962..1864
                     /locus_tag="WJK66_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HEZ7579356.1"
                     /translation="MDSLMTLLSVLIPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGANLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFASGKLMRDIWMPSENAGMYCLMLLVLLIGVQLKSSGVSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASADGVSWVKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIALLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIRGAGGLEAVP
                     VAVSFGVVVNIAAPFLMVVFSALG"
     gene            1907..2680
                     /locus_tag="WJK66_001680"
     CDS             1907..2680
                     /locus_tag="WJK66_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEZ7579357.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EDAGSLKRHHGGASSGGNLPEGLPASRQIQCQNEKNAIARLIAEHIPDESSLFVSIGT
                     TMEAVASELVRRRGSLRIITNNIHVASIVSARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            2792..4480
                     /locus_tag="WJK66_001681"
     CDS             2792..4480
                     /locus_tag="WJK66_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEZ7579358.1"
                     /translation="MLNKDQFADNHFIRTIIEDDLKSGKHEAVQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLIKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSVEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIHAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFEAGRTQSRRAAFHPNHEEMGEREVPISQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTIERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            4573..5529
                     /gene="gshB"
                     /locus_tag="WJK66_001682"
     CDS             4573..5529
                     /gene="gshB"
                     /locus_tag="WJK66_001682"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEZ7579359.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSV
                     KNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQSMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMRLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELDGRDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
BASE COUNT         1308 a         1542 c         1567 g         1242 t
ORIGIN      
        1 atttgtgcaa tgctttaatt tccccgacac gatttgaaac acagccgcct taaaacatct
       61 taagaaattt gaaagcgtaa aaatacggaa aacatcatga actttgaaaa cgacgacatt
      121 atccatgcgc cgaccacgtc ttccctgatt ctcgaagagc ggcacgattc ggagctgttc
      181 cgtgtttacg ccctgatttt ggacggcatt accgatcagg tgctgctgcc tggcaaaaag
      241 ctgaccgaat ccgaactttg ccgccagatg gtgtgttcgc gcaacaccgt ccgcggcgcg
      301 ctgtcgcttt tggcgcacga caagattgtc gatttgcaac ccaacagggg cgcgttcgtc
      361 cacgttcccg atttgaaaga aatgcaggat gtgttcaatg cgcgcatcga aatggagacg
      421 atgattttga atatcctcgc agatttgccg gatttggaaa cgcgcctcaa gccgctttat
      481 gcgatgatac ggcgcgaaga agaggcctcc ggcaggggag accgcgttgg ctggaaccgc
      541 ctgtccaatg cctttcacgt cgaactggcg cgcctggtgg gcaacgatgt gctgttcgac
      601 attatgaaca cgctgtgcgc gcggtcttcc ctgattgtcg ccgtggcggg cgtgcatcgc
      661 gaggaaaaac acgccatcaa tacgcatacg cattccgaac accgcgaaat cctcgacctg
      721 ctgctggcgg gcaggcgcaa ccgcgtggtc aaaatcctgc gccgccattt gggcaactgt
      781 atggagcgtt tggaaaagac tttggaagat tgaatgccgg agcggataag ccgcctgaac
      841 cttcaggcgg ttttttaatg gcgaacctga tgccgtctga aatatggatg cgggtatctg
      901 caattttcag acggcaattt ttaaaccgca tatatcatgc ggcaataaag gaggtcagga
      961 gatggacagc ctgatgacgt tgctttcggt attgataccg atgtttgccg gattttttat
     1021 ccgtgtgccc aagccttacc tgcccgcttt ggataaggtg ctatcggtct tggtgtatgc
     1081 tgtgctgctg ctgatcggcg tctcgttgtc gcgcgtggag gatttgggtt cgcggttgga
     1141 cgatatggcg ttgacggttc tgtggctgtt tgtttgtacg gtcggggcga acctgcttgc
     1201 tttggcagtg ttgggaaagt tattcccgtg gcggataaag gggaaaggga agggcgtttc
     1261 ggtcggtgtg tcgggcagtg tggggcagct cggatgcgtg ctgctcggat ttgcatccgg
     1321 caaactgatg cgcgatattt ggatgccgtc tgaaaacgcg ggtatgtatt gtctgatgct
     1381 gctggtgctc ctcatcggcg tacagctcaa aagcagcggc gtatcgttgc ggcaggtttt
     1441 ggtcaaccgc aggggtattc ggttgtcggt ctggtttatg ctttcatctc tttcaggcgg
     1501 gctgctgttt gccgcatcgg cagacggtgt gtcgtgggtg aaaggtttgg cgatggcttc
     1561 cggcttcggt tggtattccc tctcgggttt ggtgatgacc gaggcttacg gcgcggtatg
     1621 gggcagtatc gcgcttttga acgatttggc acgagagctg ttcgcgctgg catttattcc
     1681 gctgctgatg aagcgttttc ccgatgcggc agtgggggtc ggcggcgcga ccagtatgga
     1741 tttcacattg cccgtgattc ggggtgcggg cggcttggaa gccgtaccgg tagcggtcag
     1801 cttcggcgtg gtggtcaata tcgccgctcc gtttctgatg gtggtgtttt ccgctttggg
     1861 ctgaacgcgg taaaatcggc atcccgatgc aaggaggcag aaaacgatga aaccgaaaat
     1921 ccaaaggcac ggagagattt taagccttgt ccgccggcat cagtttatgt cggtggacga
     1981 gcttgccgcc gcattggacg ttaccccgca gacgatacgc cgcgacatcc gcgaattgga
     2041 ggatgctggc agcctgaaac gccaccacgg cggcgcatcc tcgggcggaa acttgccgga
     2101 ggggctgcct gccagccgcc aaattcaatg tcaaaacgaa aaaaacgcca ttgcccggct
     2161 gattgcggaa catattcccg acgagtcatc gctgtttgtc agtatcggca cgacgatgga
     2221 agccgtggca tcggagctgg tgagacggcg cggcagcctg cggattatta ccaacaatat
     2281 ccacgtcgcc tccatcgttt cggcgcggac ggattacacg gtcatcatca cttccggagt
     2341 cgtccgccct ttggacggcg gcattaccgg cgtggcaacc gtcgatttta tcaaccagtt
     2401 caaagtcgat tatgccgtga tgagtacgca cggcgtggaa agcgacggtt cgcttttgga
     2461 cgacgactac aaagaagtca gcgtgatgca ggcgatgatt gccaacgcgc gcgtccgctt
     2521 cctcggcgtg gaccacagta aattccgcag caacgcgctg gtcaggctcg gcgacattac
     2581 ggcgtttgac aaagtattta ccgaccggct gcccgatacc gcgatgcaga agatgctgaa
     2641 agaggcgggg gtggaatgcc tgattgccga tgccgtctga acgctatgtc aaagcgcgca
     2701 agtcgggtac aataaacaca tcatcaaacc gcttcagacg gcataaggaa ccgccccaat
     2761 gccgtctgaa gccatctgtt caaagaaaac tatgctcaat aaagaccaat ttgcggacaa
     2821 ccattttatc cgcaccatca tcgaagacga cctcaaaagc ggcaaacacg aagccgtcca
     2881 aacccgtttt ccgccggagc ccaacggcta cctgcacatc ggacacgcca aatccatctg
     2941 cctgaacttc ggtttggcgt atatttacga cggcttgtgc aacctgcgtt ttgacgatac
     3001 caaccccgaa aaagaaaacg acgaatacgt caacgccatc aaagaagatg tcgagtggct
     3061 cggtttccat tgggcgggcg agccgcgttt cgcttccaac tatttcgacc agctttatga
     3121 ctacgccgtc ggtttgatta aagacggcaa agcgtatgtc gatgatttga cgcccgaaga
     3181 aatgcgcgaa taccgcggta cgctgaccga agcgggtaaa aacagccctt accgcgaccg
     3241 cagcgtcgaa gaaaacctcg acctgttcac acgcatgaaa aacggcgagt tccccgacgg
     3301 cagcaaaacc ctgcgcctga aaatcgacat ggcatcgggc aacatcaata tgcgcgaccc
     3361 cgtcatctac cgcatccgcc gcgcccatca ccacaacacc ggcgacaaat ggtgtatcta
     3421 cccgatgtac gactacacgc attgcatctc cgatgccatc gaaggcatca cgcattcctt
     3481 gtgtacgctc gaatttgaag cacaccgtcc gctgtacgac tgggtgttgg ataacatcca
     3541 cgcgccgcat gccacccgtc cgcgccaata cgagttttcc cgtttggagc ttttgtacac
     3601 cattacctcc aaacggaaat tgaatcagtt ggttgtggaa aaacacgttt ccggctggga
     3661 cgatccgcgt atgccgacta tttccggtat gcgccgacgc ggctacacgc ccgaaggcct
     3721 gcgtctgttt gccaaacgcg ccggtatttc caaatctgaa aacatcgtcg atatgagcgt
     3781 gttggaaggc gcgattcgcg aagagttgga aaattccgcc ccacgcctga tggcggtgtt
     3841 gaacccgctc aaagtgaccc tgaccaactt tgaagccggc agaacccaaa gccgccgtgc
     3901 cgcgttccat ccgaaccacg aggaaatggg cgagcgcgaa gtacctattt cacaaaccat
     3961 ctatattgaa gccgacgact ttgccgaaaa tccgcccaaa ggcttcaaac gcctgattcc
     4021 cggcggcgaa gtacgcttgc gccacggcta tgtcatcaag tgcgatgaag tcgtcaaaga
     4081 cgaagcaggc aatgtggttg aactcaaatg cagcatcgac cacgacacct tgggcaaaaa
     4141 tccagaaggc cgtaaagtta aaggcgtgat tcattgggtt tccgccgaac acgccgccga
     4201 aatcaaagtc cgtctgtacg accgcctctt taccatcgag cgtcccgatg ccgtgcgcgg
     4261 cgaagacggc gaatacctgc cgttcaccga tttcctcaat ccggaatccg tcaaggaaat
     4321 caccgcctac gccgaacctg ccgccaaaga tttgccggca gaaagccgtt ggcagttcga
     4381 gcgtatcggc tacttcgtaa ccgaccgtaa agaccacggc aaagacacgc cggtgtttaa
     4441 ccgcacggtc acgctgaaag attcttggca gcctaagtaa aaccccatcc ttgccgtctg
     4501 aatattgttc ggacggcatt tcccctttgc ccgcgaaatg cggcacattc ggcacaccgg
     4561 aaaggaaata tgatgaaagt cctctttatc gccgacccga tggcaagttt caaaacctac
     4621 aaagacacca cctacgccat gatgcgcgag atggcaaaac gcggttggcg gctgtttcat
     4681 accttgagcg gggaattgtc ggttaaaaac ggtctggtgg ttgctgatgc gtctgctttt
     4741 gagtttctcg gcgcacaaga cgaccacgac aaaaactggt ttgccgccgc cgacaaagtt
     4801 cagacggcat tgaaagcatt tgatgccgtg attatgcgta ccgatccgcc gttcgatatg
     4861 caatacctct acgccaccca attactgacg ctggcggaac agcagggcgc gaaagtcttt
     4921 aacagcggac agtcgatgcg tgacttcaat gaaaaactgg cgattttgaa cttcagccgc
     4981 tttaccgcgc ccacgctggt aacgacccgt tccgccgatg tccgcgcatt tttgaaagaa
     5041 cacggcgaca ttatcatcaa accgctggac ggtatgggcg gtatgggtat tttccgcctg
     5101 accgaaaaag accccaacat cggcagcatc ctcgaaaccc tgatgcggct tgattcccgc
     5161 accattatgg cgcaacgcta cattcccgaa atcgtacacg gtgacaaacg catcctgatt
     5221 atcggcggcg aagtcgtccc ctatgctttg gcgcgtatcc cgcaaaacgg cgaaacacgc
     5281 ggcaaccttg cggcaggcgg acgcggcgtg gcgcaggaat tggacggacg cgaccgcgaa
     5341 attgccgaaa cgctcgcccc cgaactcaaa cggcgcggca tcctgctggc cggtttggac
     5401 gttatcggca gcaacctgac cgaagtcaac gtaaccagcc cgaccggatt ccaagaaatt
     5461 atgaaacaaa aaggtttcga cgtggcggca atgtttgccg atgccgttgc cgcgtggtcg
     5521 gtacgttaaa ccgatgccgt ttgaaaggct tttgcttcac aaccgtttgg tttgttcggg
     5581 caggcagggt tttccctcag ccggcaggga tgcggttaat gccgtacgga caccccaccg
     5641 ccccgttttc agacggtat
//
LOCUS       DAWKOG010000134         5645 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.134, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000134 DAWKOG010000000
VERSION     DAWKOG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.134"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(128..889)
                     /locus_tag="WJK66_001683"
     CDS             complement(128..889)
                     /locus_tag="WJK66_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579360.1"
                     /translation="MDANKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDASVP
                     KLDDASRNRVLKAYLKKWQEEHNAAVADCGEEIETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRIRFDAKKDSAEFETGQIEGGENADAAESGTAADGVAENGEGGAEEALMPV
                     QINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMAVFTYQL
                     QQMLHEQAGAVVEDVLEHDIRKILNDIKYELKYKR"
     gene            989..1240
                     /locus_tag="WJK66_001684"
     CDS             989..1240
                     /locus_tag="WJK66_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579361.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPAETAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            1292..2098
                     /locus_tag="WJK66_001685"
     CDS             1292..2098
                     /locus_tag="WJK66_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEZ7579362.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKISSSDDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEDGQAAK"
     gene            2405..3928
                     /locus_tag="WJK66_001686"
     CDS             2405..3928
                     /locus_tag="WJK66_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEZ7579363.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGDKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTDLLGMIGKSERGVATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPPQWQAKIGIIPVKS"
     gene            4026..5438
                     /gene="trkA"
                     /locus_tag="WJK66_001687"
     CDS             4026..5438
                     /gene="trkA"
                     /locus_tag="WJK66_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEZ7579364.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
BASE COUNT         1404 a         1702 c         1371 g         1168 t
ORIGIN      
        1 cggcttcgtc gccttgtcct gatttttgtt aatccactat attaatattt tgcgcctgtt
       61 ccatgaggct ttcaagtcgg acgggcagga aaatgccgtc tgaaacggct ttcagacggc
      121 atggcaatca gcgtttgtat tttaactcgt acttgatgtc gttgaggatt ttgcggatat
      181 cgtgttccaa cacgtcttcg actaccgcac ccgcctgctc gtgcagcatc tgctggagct
      241 ggtaggtaaa aaccgccatc tgcttttgca ccgccgtacg gatgatgccg ttgacggtat
      301 cggtcagatg cgggcgcagg cgtttgatca gccgctcggt cagctcctgt tcggacaggc
      361 agaacacttc gcgccggttg acggctttcg ggttcaggat attgatttgg acgggcatca
      421 acgcttcttc cgcaccgcct tcgccgtttt ccgcaacccc gtctgccgcc gtacccgatt
      481 ctgccgcatc ggcgttttcc ccgccttcaa tctgtccggt ttcaaattcg gcactgtctt
      541 ttttggcatc aaaccggatt ctccgccgcg attcgatgtg tttttccgaa accgacattt
      601 gcagggaagc ctgcgcgttg agccagtttt cctgaaggac gatcatcggg tcggtttcga
      661 tttcttcccc gcaatcggca acggcggcat tgtgctcctc ctgccatttc ttcagatacg
      721 cctttaacac acggtttcgg cttgcatcgt ccagtttcgg cacagacgcg tccgttccgg
      781 tttcagaagg gcgggacagc ggcgcgtaag tcggcactgc cttcatacgg cgcgtctgac
      841 gcaggttttc caaacgtttt tcccaattcg gctctttatt cgcatccatt ttcggcttcc
      901 ggttcttaat ctttgcaggc agacaaaccc gcgcccaaag cgcggtttga tataatggcg
      961 cattttaaca gattcgcgag gatacatcat gggcagcatc gaacagcgtt tggaatatct
     1021 ggaagaggcg aacgacgtgc tgcgtatgca gaaccacgtc ctgtccaccg cattcaaagc
     1081 cttaatccgc gcccttcccg ccgaaaccgc cgaaatcgcg gtcgagtcga ttcagcttgc
     1141 ttttgaggac gccttggcag aattgagcta tgaggacagc ccccataccg atttgttcca
     1201 cgacgttact tatgcgtttt tccgtgaaaa agaacgttaa ttttgtgtta aactgatttt
     1261 ttaggctttt tgattaccga aaggaatttt gatgaatatg aaaaaatgga ttgccgccgc
     1321 ccttgcctgt tccgcactcg cgctgtctgc ctgcggcggt cagggcaaag atgccgccgc
     1381 gcccgccgca aaccccgaca aagtgtaccg cgtggcttcc aacgccgagt ttgccccctt
     1441 tgaatcttta gactcgaaag gcaatgtcga aggtttcgat gtggatttga tgaacgcgat
     1501 ggcgaaggcg ggcaatttta aaatcgaatt caaacaccag ccgtgggaca gccttttccc
     1561 cgccttgaac aacggcgatg cggacgttgt gatgtcgggc gtaaccatta ccgacgaccg
     1621 caaacagtct atggacttca gcgacccgta ttttgaaatc acccaagtcg tcctcgttcc
     1681 gaaaggcaaa aaaatatctt cttccgacga tttgaaaaac atgaacaaag tcggcgtggt
     1741 aaccggctac acgggcgatt tctccgtatc caaactcttg ggcaacgaca acccgaaaat
     1801 cgcgcgcttt gaaaacgttc ccctgattat caaagaactg gaaaacggcg gcttggattc
     1861 cgtagtcagc gacagcgcgg tcatcgccaa ttatgtgaaa aacaatccga ccaaagggat
     1921 ggacttcgtt accctgcccg acttcaccac cgaacactac ggcatcgcgg tacgcaaagg
     1981 cgacgaagca accgtcaaaa tgctgaacga tgcgttggaa aaagtacgcg aaagcggcga
     2041 atacgacaag atttacgcca aatattttgc aaaagaagac ggacaggccg caaaataagc
     2101 ccgcccgtcc gaacacaatg ccgtctgaag ccctttcaga cggcattgtt catcaatcgg
     2161 cctacaatga actgcctgct gatttctccc taccgcaaag caacaggcaa agattacaaa
     2221 tatcaaaatc cgagtaaaac agtattttat taaaacaaat tgataatcaa gagattagaa
     2281 ttatgtattg tctttaccgt acaaacgctg gcactatttc aacctgataa aaaacagcct
     2341 tcaaaaaggt tgcttaaaac agcagcagac acttaccgcc acaaccttga aaaggaacac
     2401 aatcatgacc gtcatcaagc aagaagactt tatccaaagt atctgcgatg ccttccaatt
     2461 catcagctac taccatccga aagactacat cgacgcgctt tataaggcgt ggcagaagga
     2521 agaaaatccc gccgccaaag acgcgatgac gcagattttg gtcaacagcc gtatgtgtgc
     2581 ggaaaacaac cgccccatct gccaagatac cggtatcgca accgtgtttt tgaaagtcgg
     2641 tatggatgtg cagtgggatg cggatatgag cgtggaagaa atggttaacg aaggcgtgcg
     2701 ccgcgcctac acttgggaag gcaatacgct gcgcgcttcc gttctcgccg accccgccgg
     2761 caaacgccaa aataccaaag acaacacgcc cgccgtcatc catatgagca tcgtgccggg
     2821 cgacaaagtc gaagtaacct gcgcggcaaa aggcggcggc tctgaaaaca aatccaaact
     2881 cgccatgctc aatccttccg acaacatcgt cgattgggta ttgaaaacca tcccgaccat
     2941 gggcgcgggc tggtgtcctc ccggcatctt gggcatcggc atcggcggca cgcccgaaaa
     3001 agccgtgttg atggcaaaag aatccttgat gagccacatc gacattcaag agttgcagga
     3061 aaaagcagcg tcaggtgcag aattgtccac caccgaagcc ctgcgcctcg aactctttga
     3121 aaaagtcaac gcgctaggca tcggcgcgca aggcttgggc ggtctgacca ccgtgttgga
     3181 cgtgaaaatc ctcgattacc cgacccacgc cgcctccaaa ccgattgcca tgattccgaa
     3241 ctgcgccgcc acccgccacg tcgaatttga attggacggc tcaggccctg tcgaactcac
     3301 gccgccgcgc gtcgaagact ggcccgattt gacttacagc cccgacaacg gcaaacgcgt
     3361 cgatgtcgac aagctgacca aagaagaagt ggcaagctgg aaaaccggcg acgtattgct
     3421 gttgaacggc aaaatcctca ccggccgcga tgccgcacac aaacgcctcg tcgatatgct
     3481 caacaaaggc gaagaattgc ccgtcgattt caccaaccgc ctgatttact acgtcggccc
     3541 cgtcgatccg gtcggcgatg aagtcgtcgg tccggcaggt ccgaccacag ccacccgcat
     3601 ggacaaattc acccgccaaa tgctcgaaca aaccgacctc ttgggcatga tcggcaaatc
     3661 cgagcgcggc gtggccacct gcgaagccat cgccgacaac aaagccgtgt acctcatggc
     3721 agtcggcggc gcggcgtatc tcgtggcaaa agccatcaaa tcttccaaag tcttggcgtt
     3781 ccccgaattg ggcatggaag ccatttacga atttgaagtc aaagacatgc ccgtaaccgt
     3841 cgccgtagac agcaaaggcg aatccatcca cgccaccgcc ccgccccaat ggcaggcgaa
     3901 aatcggcatc atccccgtca aatcttgagg cgtcatgccg tctgaacaca aaatctgctt
     3961 tcagacggca tttccgcccc tggttgcggt acaatccacc atttcatcac tcggcgaccc
     4021 acaccgtgaa aatcctcatt ttaggcaacg gacaggtagg ttctaccgtc gcacaaaacc
     4081 ttgccgccat acccaacaac gacgtaaccg ttatcgacat cgacgaaaaa gcattgcagg
     4141 aaacaggcag ccgcctcgat gtccaaaccg ttttcggcaa cggcgcatcc cccttcacat
     4201 tagaacgcgc cggcgcggaa gatgccgact tgctgctcgc gctctcccgc agcgacgaaa
     4261 ccaacatcgt cgcctgcaaa gttgccgccg acctgttcaa catccccggc cgcatcgcgc
     4321 gcgtccgttc cagcgaatac ctcgaatacc tcagccccaa gctcgaaaac aacgaaaacg
     4381 gcagcctttc catattcggc ataaccgaaa ccatcagccc cgaacagctc gttaccgaac
     4441 agcttgccgg cctgatagac tgcccgggcg cattgcaggt tttacgtttt gcagacgacc
     4501 gcgtgcggat ggtcatcata caggcgcggc gcggcggact gcttgtcgga cgcagcattg
     4561 ccgacatcgc ccaagatttg cccgacgggg ccgactgcca aatctgcgcc gtttaccgca
     4621 acaaccgcct catcgtcccc gcgccgcaaa ccgtcatcat cgaaggcgac gaaatcctat
     4681 ttgccgccgc cgccgaaaac atcggcgcgg tcatacccga attgcgcccc aaagaaacca
     4741 gcacccgccg catcatgatt gccggcggcg gcaacatcgg ctaccgtctc gccaagcagc
     4801 tcgaacacgc atacaacgtc aaaatcatcg aatgccggcc gcgccgtgcc gaatggatag
     4861 ccgaaaacct cgacaacacc ctcgtcctgc aaggttcggc aaccgacgaa accctgctcg
     4921 acaacgaata catcgacgaa atcgacgtat tctgcgccct gaccaacgac gacgaaagca
     4981 acattatgtc cgcccttttg gcgaaaaacc tcggcgcgaa gcgcgtcatc ggcatcgtca
     5041 accgctcaag ctacgtcgat ttgctcgaag gcaacaaaat cgacatcgtc gtctcccccc
     5101 acctcatcac catcggctcg atactcgccc acatccggcg cggcgacatc gttgccgtcc
     5161 accccatccg gcgcggcacg gcggaagcca tcgaagtcgt cgcacacggc gacaaaaaaa
     5221 cttccgccat catcggcagg cgcatcagcg gcatcaaatg gcccgaaggc tgccacattg
     5281 ccgccgtcgt ccgcgccgga accggcgaaa ccattatggg acaccatacc gaaaccgtca
     5341 tccaagacgg cgaccacatc atctttttcg tctcgcgccg gcgcatcctg aacgaactgg
     5401 aaaaactcat ccaagtcaaa atgggctttt tcggataaac cgccccattc cggacacatt
     5461 gccgccaagc ggtatggaag cggaataatg gtaggtgggc ttcagacggc atcgtccccc
     5521 accgttattc ccgcgcaggc gggaatccgg tctgttcggt ttcagttgtt tttgggtttc
     5581 gggtaatttc caaatcgtca ttcccgcaca ggcgggaatc caaaccattg gacagcggca
     5641 atatt
//
LOCUS       DAWKOG010000135         5627 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.135, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000135 DAWKOG010000000
VERSION     DAWKOG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.135"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            64..324
                     /locus_tag="WJK66_001688"
     CDS             64..324
                     /locus_tag="WJK66_001688"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579365.1"
                     /translation="MENAAASPAGRFRRHSRRRKCPTLKAQTHTKNRMPAPATIRQIP
                     LFFPNRLQVSPIRRVMPPPPFKGNARVNGFAVGKAAGYRYVS"
     gene            431..955
                     /gene="nspA"
                     /locus_tag="WJK66_001689"
     CDS             431..955
                     /gene="nspA"
                     /locus_tag="WJK66_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HEZ7579366.1"
                     /translation="MKKALAALIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSTGLGVLAGVSYAVTPNVDLDAGYRYNYIGKVNTVKN
                     VRSGELSVGVRVKF"
     gene            1091..1480
                     /locus_tag="WJK66_001690"
     CDS             1091..1480
                     /locus_tag="WJK66_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ7579367.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEVRQVFQNLQAVAEAAGGTLADIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            1558..2388
                     /locus_tag="WJK66_001691"
     CDS             1558..2388
                     /locus_tag="WJK66_001691"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEZ7579368.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRIAKAESLAREAEAELRGKKHVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            2403..3860
                     /locus_tag="WJK66_001692"
     CDS             2403..3860
                     /locus_tag="WJK66_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEZ7579369.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFANHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILSAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(3901..4290)
                     /locus_tag="WJK66_001693"
     CDS             complement(3901..4290)
                     /locus_tag="WJK66_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579370.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALMGIAFLRGVDVYDPPVSKEK
                     ALKALEKHPEIYHKFKRFFPFVELPLF"
     gene            complement(4472..4873)
                     /locus_tag="WJK66_001694"
     CDS             complement(4472..4873)
                     /locus_tag="WJK66_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579371.1"
                     /translation="MKIKPLQFSNNSRRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKEENNSDGNPTANYFFEIIDDSYMDWLKEESFDF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            complement(4981..5160)
                     /locus_tag="WJK66_001695"
     CDS             complement(4981..5160)
                     /locus_tag="WJK66_001695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579372.1"
                     /translation="MPSETVFNFQATFPLFETGYWELSSSKILHLLLPRQHLGQNGRR
                     LKSKHCHQPFEDRME"
     gene            complement(5179..5556)
                     /locus_tag="WJK66_001696"
     CDS             complement(5179..5556)
                     /locus_tag="WJK66_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ7579373.1"
                     /translation="MCEFKDFRRNIPCFKEYDENSFIGKWYDDGVWDDEEYWKLENAL
                     IEVRRKYPYPMDIPRDIVIGIGSIIEFLMVPNWKLFTIKSSPWLPKSIKINERYERFR
                     VMLRYIFTEKDIVNVRFDYYNKK"
     gene            complement(5562..>5627)
                     /locus_tag="WJK66_001697"
     CDS             complement(5562..>5627)
                     /locus_tag="WJK66_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579374.1"
                     /translation="KLNRKETRNGTFDKNLNRIGD"
BASE COUNT         1401 a         1651 c         1166 g         1409 t
ORIGIN      
        1 gcaaacaaat cggcggccgc ccgggaaaac ccccccgaac gcgtccggaa aatatgctta
       61 tcgatggaaa acgcagccgc atcccccgcc gggcgtttca gacggcacag ccgccgccgg
      121 aaatgtccga cgcttaaggc acagacgcac acaaaaaacc gtatgcctgc acctgcaaca
      181 atccgacaga taccgctgtt ttttccaaac cgtttgcaag tttcacccat ccgccgcgtg
      241 atgccgccac caccatttaa aggcaacgcg cgggttaacg gctttgccgt cggcaaagca
      301 gccggatacc gctacgtatc ttgaagtatt gaaaatatta cgatgcaaaa agaaaattta
      361 agtataataa agcagaattc tttaacgaat tcttaacaat ttttctaact gaccataaag
      421 gaaccaaaat atgaaaaaag cacttgccgc actgattgcc ctcgctctcc cggccgccgc
      481 actggcggaa ggcgcatccg gcttttacgt ccaagccgat gccgcacacg caaaagcctc
      541 aagctcttta ggttctgcca aaggcttcag cccgcgcatc tccgcaggct accgcatcaa
      601 cgacctccgc ttcgccgtcg attacacgcg ctacaaaaac tataaagccc catccaccga
      661 tttcaaactt tacagcatcg gcgcgtccgc catttacgac ttcgacaccc aatcgcccgt
      721 caaaccgtat ctcggcgcgc gcttgagcct caaccgcgcc tccgtcgact tgggcggcag
      781 cgacagcttc agccaaacct ccaccggcct cggcgtattg gcgggcgtaa gctatgccgt
      841 taccccgaat gtcgatttgg atgccggcta ccgctacaac tacatcggca aagtcaacac
      901 tgtcaaaaac gtccgttccg gcgaactgtc cgtcggcgtg cgcgtcaaat tctgatatgc
      961 gccttattct gcaaaccgcc gagccttcgg cggttttgtt ttctgccacc gcaactacac
     1021 aagccggcgg ttttgtacga taatcccgaa tgctgcggct tctgccgccc tattttttga
     1081 ggaatccgaa atgtccaaaa ccatcatcca caccgacaaa gcccccgccg ccatcggcgc
     1141 gtacagccaa gccgtccgag caggcgacac cgtttacatg agcggtcaaa tccccctcga
     1201 tcccgccacg atgaccgtcg tcggcaacgg cgatttccgc gccgaagtgc gccaagtgtt
     1261 ccaaaacctg caagccgtcg ccgaagcggc aggcggcacg ctggccgaca tcgtcaaact
     1321 caacgcctac ctgaccgact tgggcaattt tgccgtcttc aacgaagtca tggcggaatt
     1381 tatcgccgag cccttccccg cccgcgccgc cgtcggcgtt gcctcgctgc ccaaaggcgt
     1441 gcaggtcgaa gccgaagccg tcctcgtttt gaacgcataa ttccacgttc ggactgcatg
     1501 atatttcaaa accatgccgt ccgaaccgca acactttccc acttcacaac atctgcaatg
     1561 gcacattacg caatcggcga catacaaggc tgtttcgacg aactgaccgc gctgctcggc
     1621 aaaatcggtt tcaaccacgg cacggacacc ctctggctga cgggcgacat cgtcaaccgc
     1681 ggcccgaaat ccctcgaaac gctgcaattc tgcatccggc acgaaaacag cgtgcaaatc
     1741 gtcctcggca accacgacct gcacctgctc gctgtcggct gcggcgaagg cgcgcccaaa
     1801 cgcagcgaca caatcgaacc catactcaaa caccccgacg gaaaaaaaat gctcgactgg
     1861 ctgcgtgcgc aaccgctgtt gatacgcgag ggcagccgcg tgatggtaca cgccggcatc
     1921 ctgccgcaat ggcgcatcgc caaagccgaa tcgctcgcta gagaagccga agccgaactg
     1981 cgcggcaaaa aacacgtcaa attcttctcc aaaatgtacg gcaacaaacc ggccgcgtgg
     2041 gacgaaggtt tggaaggcta tgcccgcctg cgcttcatcg tcaacgcctt cacgcggatg
     2101 cgcgccctga cctttaaaaa cgaactggat ttcgactaca aatccacagt gaaaaaaatg
     2161 ccgccttacc tgcgcccgtg gttcaaagcc cccgaccggc aaaacctcga ccgcaccatc
     2221 atcttcggac actggtcctc gctgggctac acgaatgccg acaacgtcat ctcgctggac
     2281 accggcgcgc tgtggggcgg acagctgacc gccgtcaacc tcgaaacgga agaaattacc
     2341 caagtccaag ccgccggcgg catagactgg aaaagcttcg caaaataacg gaccgcccca
     2401 ctatgcacaa aatcctgccc atcgcccacg tcctctcccg actgggtatg ctgttttcct
     2461 ttatcctgct gatacccgcc gccctctcct acgccttttc ggacggcgcg tacaccgcct
     2521 tcgccaccac cgcgaccgtt accctttccg gctcgtgcat cgtccggctc gccaccctcc
     2581 ggttcaggcg cgaactgcgc ccgcgcgacg gcttcaccct cgtcctgatg ttgtggctgg
     2641 cgtttgccgc tatggcggcg atgccgatgt acctgtattt cccgaatatg ggctttaccg
     2701 acgcattttt tgaatcgatg tcgggactga ccaccaccgg cgcgaccgtc atcccccacg
     2761 tcgacgggct cgccccctcc gtcaactttt ggcggcatat gctcaactgg ctgggcggga
     2821 tgggcatcat cgtccttgcc gtcgccatcc tgcctatgct cggcgtaggc ggcacgcagt
     2881 tattcaaagc cgaaatcccc ggcattgaca aagaaagcaa aatgtcgccg cgcatttccc
     2941 aagtggcgaa aaaactttgg ttcggctaca ccctgatcac catcctcgcg gcagcctgcc
     3001 tgcattttgc cgggatgggc tggttcgatg ccgtctgtca cgcaatggcg accctctcgc
     3061 tgggcggatt ttccacccac gatgccagca tagcttatta caactccccc ctcatcgagg
     3121 cggtcatcat cgttttcacc attgtcggcg gcatcaattt tgccaaccat ttcgccgccc
     3181 tcaacagccg cagcctcaaa acctattgga aagacgaaga atgccggacg atgctgctgc
     3241 tgctctccgg cagcatcctt tccgccgccc tgtacctgtg gcacaccggc tattacgccg
     3301 gttttaccga atccctgcgc tacaccgcct tcaacttcgt ctccatcgga ttggcaaacg
     3361 ggctggccaa caccgacttc gcacaatggc cgctcctgat ttccctgtgg atgtttttcc
     3421 tcgccaacat cctcgccaac tccggctcga ccggcggcgg catcaaaacc atacgcgccc
     3481 tcgtcctgtt caaattcagc ctgcgcgaaa tgatggtgct gctgcacccc aaagccgtcc
     3541 gcaccgtcaa aatcagcggc aaggccatac ccgaccgcct cgcgctgacc gttatgtcct
     3601 tcatcttcat ctactttatg accgtcgtcc tcttcagctt cctgctgatg gcgagcggta
     3661 tggaatttac caccgccttc accgccgtca tcgcctgcat caccaacgcc ggccccggcc
     3721 tgggcgaagt cgggcccgcc ggcaattacg ccggtttgga cgtgatgcag aaatggatct
     3781 gcgttaccgc catgctcttg ggcaggctgg aaatctttac cgtcttcatc ctcttcaccc
     3841 ctgcctactg gaagaaataa aaaatgccgt ctgaaaactt ttcagacggc atattgtctt
     3901 ttaaaaaagc ggaagctcta caaaaggaaa gaaccgttta aatttatgat aaatttcagg
     3961 atgtttctct aaggctttta atgctttttc ttttgaaacg ggagggtcat agacatctac
     4021 cccccttaag aaggcaatgc ccattaatgc atttttttga tcatttgaaa tgtaaccata
     4081 attaacgact ccaattacat cataaatttc ccctatactc ttatcaaaat caatcaaatc
     4141 aaaaatatac aatggaattg tatcaatagg agtatctctg acaactatat ccaaccataa
     4201 tttaaattca gaaagattaa tagcatcata gaacatacag actattgcaa atcctaaatc
     4261 ttcacaattt tctttactaa ttttccacat aattatttac ctttatccat tgcttcccaa
     4321 ccgatacatc cggtatttta gcttctcctt ttccgtcaaa cactcctttg gttacctgct
     4381 ttgtatcaaa aatgccgtct gaaagccgaa tattgtttca gacggcattt tgattgttgg
     4441 gtttttaatc agagctttgt ttgataattt gttattttga ataaaataca ggttctgtcg
     4501 agctgataac ttctattaca gaatcgctaa aaaagaaaat ataagcctta taatatttct
     4561 tttcaaaaaa atcaaaactt tcttctttca accagtccat ataactgtca tctattatct
     4621 cgaaaaaata atttgcagta ggattcccat ctgaattatt ttcttcttta ttttcaaata
     4681 aatattctga aaattttact ttataagatt catctgttat ttgaaaatga attatattgt
     4741 caataaatat ttcaatattt aattcataat ttcggtcgga tgccaaaatg ttcacatctt
     4801 gttcggtaac acaaattttg tctacgtaaa atctgcgact attgttagaa aattgtaatg
     4861 gtttaatctt cactatcgtt ttccatatct gatttttgtt ttggtaacac gacgatcggg
     4921 gtttcagcga caccgaggta ttttaccgtc taatcgtgga gattttaccg attttctttc
     4981 ctattccatc cggtcttcaa atggttgatg acagtgtttg cttttcagcc gtctgccgtt
     5041 ctgaccaagg tgctgccgtg gaaggagcag gtgtaggatt tttgaagaac ttagttccca
     5101 atatcctgtt tcaaataagg ggaaggtcgc ctgaaaatta aacacggttt cagacggcat
     5161 tttgattgcc ggggtttgct attttttgtt gtaataatca aatcgtacgt tgactatgtc
     5221 tttctcggta aaaatataac ggagcattac tctgaacctt tcataacgct cattaatttt
     5281 gatactttta ggcaaccaag gggaagattt aattgtaaaa agtttccaat ttggaaccat
     5341 taaaaactca ataatgctac cgattccaat cacgatatcc cttggtatat ccatcggata
     5401 aggatatttt ctcctaacct cgattaaagc attctccaat ttccaatatt cttcatcatc
     5461 ccacaccccg tcatcatacc atttgccaat aaatgaattt tcgtcatact ccttaaaaca
     5521 agggatgttt cttctaaaat ccttgaactc gcacatcata attaatctcc aatacgattt
     5581 aggtttttat caaatgtacc gtttcttgtt tcttttctgt tcagctt
//
LOCUS       DAWKOG010000136         5603 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.136, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000136 DAWKOG010000000
VERSION     DAWKOG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.136"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            72..998
                     /gene="fabD"
                     /locus_tag="WJK66_001698"
     CDS             72..998
                     /gene="fabD"
                     /locus_tag="WJK66_001698"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEZ7579375.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFAEHAIVKNTFAEASAILGQDLWA
                     MINGSDAKIIGQTVNTQPIMLAAGVAVYRAYSEAGGKTPAAVAGHSLGEYTALVAAGA
                     LDFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVKQICAESTQGEVVEAVNFN
                     SPGQVVIAGNTAAVERAMLAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQVAAFIETH"
     gene            1137..3002
                     /gene="msbA"
                     /locus_tag="WJK66_001699"
     CDS             1137..3002
                     /gene="msbA"
                     /locus_tag="WJK66_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEZ7579376.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAINRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDGFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLSALQAANLQSLIDASPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            3102..4667
                     /gene="guaA"
                     /locus_tag="WJK66_001700"
     CDS             3102..4667
                     /gene="guaA"
                     /locus_tag="WJK66_001700"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEZ7579377.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPILGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSELTRGIQDDTPNTLDVWMSHGDKVSKLPTGFTVIGDTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            4729..5475
                     /gene="fabG"
                     /locus_tag="WJK66_001701"
     CDS             4729..5475
                     /gene="fabG"
                     /locus_tag="WJK66_001701"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEZ7579378.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRSGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMVREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
BASE COUNT         1371 a         1796 c         1363 g         1073 t
ORIGIN      
        1 ttttgtcccg cctgaatttt tcgtaaaact cgggcagaat acctgattat ccaaccaaac
       61 aaaggaatac tatgtctttt gccttctttt ttcccggaca aggttcccaa agcctcggta
      121 tgatgaacgg ctttgccgaa cacgccatcg tcaaaaacac ctttgccgaa gcctccgcca
      181 tattggggca ggacttgtgg gcgatgatta acggcagcga tgccaaaatc atcggacaaa
      241 ccgtcaatac ccagcccatc atgctcgccg ccggcgttgc cgtttaccgc gcctattcgg
      301 aagcgggcgg caaaacgcct gccgccgttg ccggacacag cctcggcgaa tacaccgcac
      361 tcgttgccgc cggcgcattg gattttgccg acgcggtcaa actcgtgcgc ctgcgcgccg
      421 aactgatgca gtctgccgta ccgcaaggcg tgggcgcaat ggcggcgatt ctcggcttgg
      481 aagatgaaca ggtaaaacaa atttgcgccg aatccacaca aggcgaagtg gttgaagccg
      541 tcaacttcaa ctcgcccgga caagtcgtga ttgcaggcaa taccgccgcc gtcgagcgcg
      601 ctatgcttgc cgccaaagaa gccggcgcca aacgcgccct gccgctgccc gtgtccgtac
      661 cttcccattg cagcctgatg aaacccgccg ccgacaaact cgccgaagcc ctgaaaaccg
      721 ttgaaatcaa gcagccgcaa atccgcgtta tccacaacgc cgacgttgcc gcctacgatg
      781 atgccgacaa aatcaaagac gcgctcgtcc gccagcttta cagccccgta cgctggacgg
      841 aaaccgtcaa cgccctcgtt tcagacggca ttgccgaatc cgccgaatgc ggcccgggca
      901 aagtgttggc gggcttggca aaacgcatca acaaagccgc cgcgtgcagc gcactgaccg
      961 atgccggaca ggttgccgcc tttatcgaaa cgcactgact tcgttccgca aaaagcagcc
     1021 tgcatgcctc aggctgcttt tcacgtccga acggcaaaca gccgcatatt tgcgctataa
     1081 ttgatgacga ccaaaccacc gacagcggct gccgttgcag ttcccgcccc accgacatga
     1141 tagaaaaact gactttcgga ctgtttaaaa aagaagacgc gcgcagcttt atgcgcctga
     1201 tggcgtacgt ccgcccctac aaaatccgca tcgttgccgc cctgattgcc attttcggcg
     1261 ttgccgccac cgaaagctac cttgccgcct tcatcgcccc cctgattaac cacggctttt
     1321 ccgcacctgc cgcgccgccc gagctgtctg ccgccgccgg catcatttcc accctgcaaa
     1381 actggcgcga acagtttacc tatatggttt gggggacgga aaacaaaatc tggaccgtcc
     1441 cgctctttct catcattctc gtcgtcatcc gtggcatctg ccgctttacc agcacctatc
     1501 tgatgacttg ggtctccgtg atgaccatca gcaaaatccg caaagatatg tttgccaaaa
     1561 tgctgaccct ttcctcccgc taccatcagg aaacgccgtc cggcaccgta ctgatgaata
     1621 tgctcaacct gaccgaacag tcggtcagca acgccagcga catcttcacc gtcctcacgc
     1681 gcgacacgat gattgttacc ggcctgacca tcgtcctgct ttacctcaac tggcagctca
     1741 gccttatcgt cgtcctgatg ttccccctgc tctccctgct ctcgcgctac taccgcgacc
     1801 gtctgaaaca cgtcatttcc gactcgcaaa aaagcatagg cacgatgaac aacgtgattg
     1861 ccgaaaccca tcagggacac cgcgtcgtca agctgttcaa cgggcaggcg caggcggcaa
     1921 accggttcga cgcgatcaac cgcaccatcg tccgcctcag caaaaaaatc acgcaggcaa
     1981 cggcggcaca ttccccgttc agcgaactaa tcgcctcgat cgccctcgcc gtcgtcatct
     2041 tcatcgccct gtggcaaagc caaaacggct acaccaccat cggcgaattt atggcattca
     2101 tcgtcgcgat gctgcaaatg tacgccccca tcaaaagcct tgccaacatt agcatcccta
     2161 tgcagacgat gttcctcgcc gccgacggcg tatgtgcatt tctcgacacc ccgcccgaac
     2221 aggacaaagg cacgctcgca ccgcagcgtg tcgaagggcg catcagcttc cgcaacgtcg
     2281 atgtcgaata ccgttcggac ggcatcaaag ccctcgacgg cttcaacctc gacatcagac
     2341 aaggcgaacg cgtcgccctg gtcggacgtt ccggcagcgg caaatccacc gtcgtcaacc
     2401 tgctgccccg ctttgtcgaa ccgtctgccg gcaacatctg catagacggt atcgacatcg
     2461 ccgacatcaa actcgactgc ctgcgcgccc aatttgccct cgtctcccaa gacgtattcc
     2521 tgtttgacga caccctgttt gaaaacgtcc gatatagccg tcccgacgcg ggcgaagccg
     2581 aagtcctatc cgccctccaa gccgctaacc tgcaaagcct gatcgacgca tcaccgctcg
     2641 gactgcacca gcccatcgga tcgaacggca gcaacttatc cggcggacag cggcaacgcg
     2701 ttgccattgc ccgcgccatt ttgaaagacg cgccgatatt attattggac gaagccacca
     2761 gcgcattgga caacgaatcc gaacgcctcg tccaacaggc gctcgaacgc ctgatggaaa
     2821 accgcaccgg catcatcgtc gcccaccgcc tgaccaccat cgaaggggcc gaccgcatca
     2881 tcgtaatgga cgacggcaaa atcatcgaac aaggcacaca cgaacaactg atgtcccaaa
     2941 acggctacta cacgatgtta cgcaatatct caaacaaaga tgccgccgtc cggacggcat
     3001 aaacaaaatg ccgtctgaaa tggtacaatc gccccgaccc tttcagacgg catcatatcc
     3061 gccgacccat ccgattatct tcaatcactg taaaacccat tatgacccaa gacaaaatcc
     3121 tcatccttga cttcggttcg caagttaccc agctcatcgc ccgccgcgtg cgcgaagccc
     3181 acgtttactg cgagctgcat tccttcgata tgcctttgga cgaaatcaaa gccttcaacc
     3241 ccaaaggcat catcctttcc ggcggcccta attccgttta cgaatccgac tatcaagccg
     3301 ataccggtat ctttgacttg ggcattccga ttttgggcat ttgctacggc atgcaattta
     3361 tggcgcacca cttgggtggt gaagtgcaac ccggcaacca gcgcgaattc ggttacgcgc
     3421 aagtcaaaac catcgacagc gagctgacac gcggcattca agacgatacg ccaaacacgc
     3481 tcgacgtatg gatgagccac ggcgataaag tttccaaact gcctaccggt ttcaccgtca
     3541 tcggcgacac gccgtcctgc ccgattgcca tgatggaaaa cgccgaaaaa caattctacg
     3601 gcatccaatt ccaccccgaa gtaacccaca ccaaacaagg ccgcgccctg ttgaaccgct
     3661 ttgttttgga tatttgcggc gcacaaccgg gctggacaat gccgaactac atcgaagaag
     3721 ccgttgccaa aatccgcgaa caagtcggca gcgacgaagt gattttaggc ttgtccggcg
     3781 gcgtggactc ttccgtagcc gccgcgctga tccaccgcgc catcggcgac caactgacct
     3841 gcgtgttcgt cgatcacggt ttgttgcgcc tgaacgaagg caaaatggtg atggatatgt
     3901 tcgcccgcaa cttgggtgtg aaagtgattc acgtcgatgc cgaagggcag tttatggcga
     3961 aactcgccgg cgttaccgac cctgagaaaa aacgcaaaat catcggcgcg gaatttatcg
     4021 aagtatttga tgccgaagag aaaaaactca ccaacgccaa atggctggcg caaggcacga
     4081 tttaccccga cgtaatcgaa tccgcaggcg cgaaaaccaa aaaagcccac gccatcaaat
     4141 cccaccacaa tgtcggcggc ctgcctgaaa acatgaagct caagctgctt gagccgttgc
     4201 gcgacttgtt taaagacgaa gtgcgcgaac tgggcgttgc tttgggtctg ccgcgcgaaa
     4261 tggtgtaccg ccatccgttc cccggccccg gtttgggcgt gcgcatcttg ggcgaagtga
     4321 aaaaagaata cgccgactta ttgcgtcaag cagacgatat tttcatccaa gaattacgca
     4381 acaccaccga cgaaaacggc acatcttggt acgacctgac cagccaggca tttgccgtat
     4441 tcctgcctgt gaaatccgtc ggcgtaatgg gcgacggacg cacttacgac tacgtcgtcg
     4501 cactgcgcgc agtcatcacc agcgacttca tgaccgcaca ctgggcagaa ctgccttact
     4561 cactgctcgg acgcgtgtcc aaccgcatca tcaacgaagt caaaggcatc aaccgcgtgg
     4621 tgtacgatgt cagcggcaaa ccgcctgcca ccatcgagtg ggaataacca gcaaacaggg
     4681 ctgcaccgtc cggcgcagtc tttcgattat cggaaaaaag gaaaaaatat gagcacacaa
     4741 gatttaagcg gcaaaatcgc tttggtaacc ggcgcatcgc gcggtatcgg tgcagcaatt
     4801 gccgacacac tggcggcagc cggtgccaaa gtcatcggta cggcgaccag tgagagcggt
     4861 gcggcggcga ttagcgagcg gttggcgcaa tggggcggcg aaggccgcgt attaaattcc
     4921 gccgaacccg aaaccgtcga aaacctgatt gccgacatcg aaaaaacgtt cggcaaactc
     4981 gatattctgg tcaacaacgc cggcatcacc cgcgacaacc tcctgatgcg catgaaagaa
     5041 gaagagtggg acgacatcat gcaggtcaac ctcaaatccg tgttccgcgc ttctaaagcc
     5101 gttttgcgcg gtatgatgaa acaacgttcc ggccgcatca tcaacatcac atccgtcgtc
     5161 ggcgtgatgg gcaatgccgg tcaaaccaac tatgccgcgg caaaagcagg cttaatcggt
     5221 ttctccaaat ccatggtgcg cgaagtcggc agccggggca ttaccgtcaa ctgcgtcgcc
     5281 cctggcttta tcgataccga catgacacgc gccctgccgg aagaaacccg ccaaaccttt
     5341 accgcccaaa ccgccttggg cagattcggc gacgcgcaag acatcgccga tgcggttctg
     5401 ttcctcgctt ccgaccaagc aaaatacatc accggccaaa cgctgcacgt caacggcggt
     5461 atgctgatgc cctgacgggc agccgtaccg acaatgccgt ctgaagccct ttcagacggc
     5521 atttgcattc tcaggcaaaa tgaacacacg ccacaccccg ccctgcccct gcggatcagg
     5581 cacaagctga gacctttgca aaa
//
LOCUS       DAWKOG010000137         5529 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.137, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000137 DAWKOG010000000
VERSION     DAWKOG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.137"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            65..490
                     /locus_tag="WJK66_001702"
                     /pseudo
     CDS             65..490
                     /locus_tag="WJK66_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
     gene            496..1726
                     /locus_tag="WJK66_001703"
                     /pseudo
     CDS             496..1726
                     /locus_tag="WJK66_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777561.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            1784..2404
                     /locus_tag="WJK66_001704"
     CDS             1784..2404
                     /locus_tag="WJK66_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ7579379.1"
                     /translation="MTTHHVELRKVTKRFGAQKAVNQVDLVLKAGESVGLAGHNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLTGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQAAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGLDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIIVD"
     gene            2415..2546
                     /locus_tag="WJK66_001705"
                     /pseudo
     CDS             2415..2546
                     /locus_tag="WJK66_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            2543..3037
                     /locus_tag="WJK66_001706"
     CDS             2543..3037
                     /locus_tag="WJK66_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEZ7579380.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTKWIDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKDGKVVSFDDMPDT
                     YIFK"
     gene            complement(3171..4832)
                     /locus_tag="WJK66_001707"
     CDS             complement(3171..4832)
                     /locus_tag="WJK66_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEZ7579381.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GIGKDGEPTDSFQPGMELWAQQTLFAEGCRGSLSKQIIERFKLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGVLIGDAAGFLNMP
                     RIKGIHTAMKSAMIAAEAVFPLLEHLEEAEGFDSGKEAADYQQRFEQSWLYRELYAAR
                     NVRPSFKWGVYLGSIYTGIDQMIFRGKAPWTLKHYGKDNEQLKKAAACKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIVE
                     ENGSPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
     gene            complement(4962..5505)
                     /locus_tag="WJK66_001708"
                     /pseudo
     CDS             complement(4962..5505)
                     /locus_tag="WJK66_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238846.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
BASE COUNT         1330 a         1508 c         1403 g         1288 t
ORIGIN      
        1 tatcccgtat gcggattggt tttaagattt gtaaatttaa tttgcatcaa aaaatcgccg
       61 atagatgatt catataatat caatattaaa gagtatcggt atatcgggga tagtcatgtc
      121 ctgtttttca atcaaacgta tgtccgcgtt tcgggcgcgg ataacagtgt tttttaccgc
      181 ctttgtcttt ttgacggcgg cactgcccgc ttatgcggag cgtctgcctg attttctggc
      241 gaaaatacag ccttcggaaa tttttccggg tgcggaccgt tacggcaagc cggaaggcaa
      301 gcctatggtt gcccgcgttt acaaaggcga tgagcagttg ggcttggtct atatcacgac
      361 cgatgcggtc aatacgcgcg gttattcgag caaaccgatc gatacgctga tggctttggc
      421 aaatgacggc acgatagccg gagcgaaact ggtcgatcat cacgaaccga ttatgctgat
      481 cggtatcccg catttgcccg cgcccgggcg ggcgatacgc ttaaactggc ttccggcgta
      541 tatcaaacca aactttatat cgacaaaccg attacgattg aagggcctgc cgaccgttcc
      601 gcaaccatcg aaggggacag gagcggccgc accatagccg tacacgcgcc ggacgtaacg
      661 ctccgcaacc tgaccgttac ccgttccggt atgagcctgc cagcaatgga tgccggtatt
      721 tatctcgaag aaaccgcccc gcgcgccctg attgaacaca acaatatttt ggataattcg
      781 gtcggcgtat atctgcatgg ttctgccgat gcgatggtgc gcgagaataa aattgtcggc
      841 gacgcgactt tgcgcgtgaa cgagcgcggc aacggcgtta ccgtttggaa cgcacccggc
      901 gcgcaggtcg tcggcaacga catttccaaa gggcgggacg gcattttttc caataccagc
      961 acgcacaaca cctacaaaaa caaccgcttc agcgatttgc gtttcgccgt ccactatatg
     1021 tacaccaacg acagcgaaat cagcggcaat atttccgtgg gcaacaatat aggctatgtg
     1081 ctgatgtttt ccgagcggct caaagtattc gacaatatcg ccgtcggcag ccgcgatcag
     1141 ggcattatgc tcaactatgt caactattcc gatattcacg acaacattat caacaaggca
     1201 ggcaagtgcg tatttgccta taatgccaac tacgataaac ttttcgccaa tcattttgaa
     1261 aactgccaaa tcggcataca ctttaccgcc gccatcgaag gcacgtccct gcacgacaat
     1321 tccttcatca acaacgaaag ccaggtcaaa tacgtcagca cgcgctttct cgactggagc
     1381 gagggcggac acggcaacta ttggagcgac aacagcgcgt tcgatttgaa cggcgacggc
     1441 ttcggcgaca gcgcgtaccg gcccaacggc atcatcgacc aaatcatctg gcgcgcgccc
     1501 gtattgcgcc tcttgatgaa cagtctcgca atcagcatcg tcaaatgggc gcaggcgcag
     1561 tttcccgccg ttctgcccgg cggcgtggtg gacagcaaac cgctgatgaa gccttatgcc
     1621 ccccaaaatt caaacccgtt atcaggcgat gaaggacgag ttgctcaaag aagccgaaac
     1681 gcggcagtcg gaatggggca gggcggaaaa cggttctttg aactagtctg tttcagacgg
     1741 catccggatt caaatgccgt ctgaaaacac aaaaggaaaa accatgacca cacatcatgt
     1801 cgaattgagg aaggtaacca aacggttcgg ggcgcaaaaa gccgtcaacc aagtcgattt
     1861 ggttttgaag gcaggagaaa gcgtcgggct tgccggacac aacggcgcgg gcaagtccac
     1921 cattatgaag ctgatactcg ggctgattac cccgaccgaa ggcgaagtga tgcttttggg
     1981 cgaacgtacc ggcagcaaag cgggggcgcg gcttcgcagc caaatcggct acctgcccga
     2041 aaccgttgcg ctgcaccctt cgctgaccgg catcgaaacg ctggattttt atgccaaact
     2101 taaaaaacag ccgctcacgc agaaccgggg gctgcttgag cgcgtcggca tttcacaggc
     2161 ggcacaccgc cgcgtcggca cttattctaa agggatgcgc caacgccttg ccttggcaca
     2221 agccctgctg ggcgagccca aagtcctgct gtttgacgaa ccgacaaccg gcctcgaccc
     2281 tgcatcaagg caaatgtttt acgaagtcgt gcgcgaactc aacgggcgcg gcgcgaccgt
     2341 attgctcagc acccacgccc ttgccgagtt ggacggacac gccgaccgca ttatcgtgga
     2401 ttaaatttaa tccactatat gcgggtatgg cgggtttgag cggacaaatc agcctgaccg
     2461 tccccgtttt gctgaccgct caggttttat gggttatcat tccgcttgtt ttggcagccg
     2521 gaatttttag aaagcgacaa atatgaaaaa aaccctgttg gcaattgttg ccgtttccgc
     2581 cttaagtgcc tgccggcagg cggaagaggg gccgccgcct ttaccccggc agattagcga
     2641 ccgttcggtc ggacactatt gcagtatgaa cctgaccgaa cacaacggcc ccaaagccca
     2701 gattttcttg aacggcaaac ccgatcagcc cgtttggttc tccaccatca agcagatgtt
     2761 cggctatacc aagctgcccg aagagcctaa aggcatccgc gtgatttacg ttaccgatat
     2821 gggtaatgtt accgattgga caaatcccaa tgccgacacg aagtggatag atgcgaaaaa
     2881 agccttttat gtcattgaca gcggttttat cggcggtatg ggtgcggaag acgcactgcc
     2941 gttcggcaac aaagagcagg ctgagaaatt tgcaaaggat aaagacggta aggttgtcag
     3001 tttcgacgat atgcctgata cctatatttt caaataaatc caaacaaaaa tgccgtctga
     3061 acgttgaagc caaggctcag acggcatttt ttgtcatagg ttcaaacggg gcaggtttga
     3121 acaactgccc cattccttat tcctgaccgg gcgtaacggc ggaataaggt tcacatcccg
     3181 ccgtaattcg gcccgcttgc gccttcggga caaatccaag tgatgttttg cgtcgggtct
     3241 ttgatgtcgc aggttttgca gtgtacgcag ttggcggcgt tgatttgcag gcgcggactg
     3301 ccgttttctt cgacgatttc atacacacca gccggacaat agcgcgtttc gggcgaggcg
     3361 tattctttat agttcacgtc tatcatcgtt tgcggattgt tcagcaccaa atggtcgggc
     3421 tggttttctt cgtgcgcgag attggcaagg aagacgctgc tcaagcggtc gaaggtcaac
     3481 acgccgtcgg gtttcggata atcaatcggt ttgcacgcgg cggctttttt gagctgctcg
     3541 ttgtctttgc cgtaatgttt caaggtccac ggggctttgc ctctgaaaat catctggtcg
     3601 atgccggtat agattgagcc gaggtaaacg ccccatttga atgacggacg gacattgcgc
     3661 gcggcgtaaa gctctcgata cagccagctt tgttcaaaac gttgctgata atccgccgct
     3721 tctttgccgc tgtcgaaacc ctccgcttct tcaaggtgtt ccaacaaggg gaacacggct
     3781 tcggcggcga tcatagcgga tttcatcgcg gtatggatgc ctttgatgcg cggcatattg
     3841 aggaaacctg ccgcatcgcc gattaaaacg ccgcctttga acgagagctt cggcaaactt
     3901 tgcaaaccgc cttcaatcag cgaacgcgcg ccgtaagcaa tgcggcggcc gccttcaaag
     3961 gttttgcgga tttcgggatg ggttttgaaa cgttggaact cttcaaacgg cgacagataa
     4021 ggattttgat agtccaaacc gaccacgaag ccgacggcga ctttgttgtc atcgaaatgg
     4081 taaacaaacg cgccgccgta ggttttgctg tccagcggcc agcctgcgct gtgcaccacc
     4141 aaaccgggct gatgctgttc ggacggcact tcccaaactt ctttaatgcc caagccgtaa
     4201 gtttgcggct ggctgttttg gtcgagtttg aagcgttcga tgatttgttt ggaaagcgaa
     4261 ccgcgacaac cttcggcaaa cagggtttgc tgcgcccaaa gctccatgcc gggttggaat
     4321 gaatcggtcg gctcgccgtc tttgccaatg cccatattgc cggttgcaat gcctttgacc
     4381 gaaccgtctt cgtgatacag cacttcggcg gcggcaaagc ccggatagat ttccacgccc
     4441 atattttccg cctgctccgc caaccagcgc acgacttcgc ccaagctgac gatgtagttg
     4501 ccgtgattgt cgaaatttgg ggtaataggc agattgaacg cttttttctc agtcaggaac
     4561 aacactttgt cctgcgttac cgtacgcgtc agcggcgcgc ctttttcttt ccagtcggga
     4621 atcaactcat tcagcgcaat cggatcgata accgcgcccg ccagcgaatg tgcacccgcc
     4681 tccgaacctt tctccaccac gcaaacgctg atttcgcgcc cgttctgttc ggcaagctgc
     4741 ttgagtttga tggcggcgga caaacccgac gggcctgcgc cgacaatcac gacatcgtat
     4801 tgcatactgt cgcgggtgat ggattctgtc atggcggttc ctgtgtattt attattgaat
     4861 tgcaaatccg taattataca acgggaacat atagttacca aatacaacaa aggtcgtctg
     4921 aaaaccatat tttcggtttt cagacgacct ttgtcgaaat ttcaataagc acgccaccat
     4981 tttacctgtc cgaccgcaaa ctccgtctga cgtttcggac tgcgtgtgaa aaacgcctta
     5041 tccccgccgg catccctccc tttcggcaca accgccaaaa tcttacctgc caaatttccc
     5101 tcacgggttt gccaagcatc caaaaactgc gccctgctca tcgaaacatg acccagcgac
     5161 gggtcggcaa gcaaaaccgt attgccgtct ataccgcgca ataccgagaa atgatcatcc
     5221 ttgcggtatt tcagatacac gatgacgggg atttgcaact gtgcaagctg ctcgaaagac
     5281 agggcatagc ctttcgcttc aaaacccaaa tcaggcataa tgcgccgcat atcctcaaac
     5341 gacgcgcgca tctgctcctt atccagcttt ttcaacacgt cctcttccgt cagcttttgc
     5401 ccgtgaaaat tgttcaaaag ccgtcgccac cgaagccgcc ccgcaggaaa aatccaaatc
     5461 ctgctttaca atattgaaat cgcgcctttc tttccaactc tgcactttga tttttccata
     5521 agcaacagg
//
LOCUS       DAWKOG010000138         5400 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.138, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000138 DAWKOG010000000
VERSION     DAWKOG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.138"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(180..563)
                     /locus_tag="WJK66_001709"
     CDS             complement(180..563)
                     /locus_tag="WJK66_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579382.1"
                     /translation="MEKIKNYIEFKKLFKNITEKFVRDLDITLIEAAEYSIIEFEIYL
                     SEYPEDSTFIFTAWAIYLLENNCLNERGKDNFFIAEMKKCYQQTNLDRIKSIYSSSDY
                     HIFAKDVNKIQQTFKLNLANIMQQE"
     gene            complement(568..1075)
                     /locus_tag="WJK66_001710"
                     /pseudo
     CDS             complement(568..1075)
                     /locus_tag="WJK66_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675736.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
     gene            complement(1707..2102)
                     /locus_tag="WJK66_001711"
     CDS             complement(1707..2102)
                     /locus_tag="WJK66_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579383.1"
                     /translation="MNQFCLSEYEKAFLCRIIENNMIWKEYLIEQIENSIIVSRDNTG
                     CGCYIDFQVNLERTNLNKYENIPIFFEGDITAPDKSDAATFLLYTTESLNKAGRIIDF
                     LEVAIAGTNYDEVEVYSWLKMENSLRFGL"
     gene            complement(2114..2827)
                     /locus_tag="WJK66_001712"
                     /pseudo
     CDS             complement(2114..2827)
                     /locus_tag="WJK66_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236085.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
     gene            complement(2923..3195)
                     /locus_tag="WJK66_001713"
     CDS             complement(2923..3195)
                     /locus_tag="WJK66_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579384.1"
                     /translation="MNKRMKMCPACQQGYLYHSKPKYLHDEIILCDECDAVWLKGMNI
                     FYGEYEKDFYSYVPFMESQGITSECIWEGDLFDHPYYEDENSNDMD"
     gene            complement(3383..3871)
                     /locus_tag="WJK66_001714"
     CDS             complement(3383..3871)
                     /locus_tag="WJK66_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579385.1"
                     /translation="MKKDIFYCEQWSYGYKKLHKPFSEKQAEEKHLKGELYTAVIGSA
                     TQPEYVITLREEVGFFSVHFFDKFGRDYLTHQFQKYSNSNYYFLSMAVWRDYITLESH
                     DLAEGYTYFFNENTDDCYVLKQDFINNERYEKTELYPQKDKVILFPKFGEYDLVLNPD
                     II"
     gene            complement(3868..>5400)
                     /locus_tag="WJK66_001715"
     CDS             complement(3868..>5400)
                     /locus_tag="WJK66_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ7579386.1"
                     /translation="KQEGMTPAAAAVVVIVVTVLTYGALSAPAAAGTAGAAGAGAGGA
                     AAGTAAGTGVAAGTAATTGVAAGTSAAAITTAAGKAALASLASQAAVSLINNKGDINH
                     TLKELGKSSTVRQAATAAVTAGVLQGISGLNTQAAEAVSKHFHSPAAGKLTANLINST
                     AAASVHTAINGGSLKDNLGDAALGAIVSTVHGEVASKIKFNLSEDYIAHKIAHAIAGC
                     AAAVANKGKCRDGAIGAAVGEMVGETLLDGHDVGKLSPQERQKVIAYSQIIAGSAVAL
                     VKGDVNTAANAATVAVENNSLLARRRVNIRWTPRQELEHEYAILEIQAITNQIRRLDP
                     KFNGIAIMRNPREPWTRHDVQTYRQYYNQLRESRGIPVLPIYRMRINNGNEFNRIMSS
                     KYPYNELYVANPKSVTGHFRVDSYNPATEEIISRKFTQFSQIQESTGIGYIKEAVRKY
                     SPGAVISNVPSTPTTIRGRKLEGKLILEVPAQVNPIPQSVLRAAQEENVIIRDTTGRI
                     YK"
BASE COUNT         1452 a         1167 c          980 g         1801 t
ORIGIN      
        1 tgtgagcttt tcagacggca ttgagccgta aatcatggaa cgcgtgcgtg ctggagcaca
       61 caccttacgc atggatttta ggtttcatgc aggctacagc ttgctgctgc ggtataaaag
      121 tatttttagt gatttactgt ttttggtatt tttgcccgac ggggttaaaa atacagttgc
      181 tactcttgct gcatgatatt ggcaagattt aatttaaatg tttgttgaat cttgtttaca
      241 tccttagcaa aaatatgata gtcactcgaa gaatagatag attttattct atccaaatta
      301 gtttgttgat aacatttttt catttccgcg atgaaaaaat tatcttttcc tctttcattg
      361 agacagttat tttctaacag atagattgcc catgctgtaa aaataaatgt actatcttcc
      421 ggatattcag ataaataaat ttcgaattca attatagaat attcagctgc ttcaattaat
      481 gttatatcta aatccctgac aaatttttcc gttatatttt taaataattt cttgaattct
      541 atataatttt ttattttttc catgattcta ttttcctgta aaaatattta attgaatatt
      601 tggatagcga tttctaaact ctaaaataac attgctgcaa gattgacagg cctttaattc
      661 tgtaaataga tcaattctac ctgatacatt acgattattt ccgagttgct gtgctatagt
      721 ttcaagtaat ttatattctc catctatatt tcttaatgtt cctctaggag gagaggcaat
      781 attatgatat ttatcaacag atataggttt aaaaattttt gtttcaggta acgaaataaa
      841 tccatgttcc cctttttgga aagaactaaa tgcttccatc ctttgcggta atcctttaac
      901 atcaattttt gctatcgcaa gattccctat ttgtcgatac tcttccggca gtttcatgcg
      961 gactctctgg ctttcagcag ttaaaagcag gagcactgtc attgctcaga taacgggtaa
     1021 caggtgtagg cttggccgat attgcagcct gtgcagatac agatcctgca accatcaatg
     1081 tgccattaac aacctgaact gtaccatgca gatattctcc attagagatt gctttcgctc
     1141 cctgtccaat gttatatccc cctgctgcca cagtagctgc tttgacactg aagccaagcg
     1201 gtccgtttaa taagtaaagt gcaggttgtg cggcaacagc tgatttgtaa tcaaaagaat
     1261 cataacgact caatgtttga taggctttcg ataaagtgtt attaatatct ttggaaggaa
     1321 tagtgagatt acccattgac tggggcgttt tcagtatcga agctgcccaa gctgtgttac
     1381 ctccagaggc ttggttaata aaatatgtta cctatgtata ttgtttcttc aaaaatcaac
     1441 cgtattgtcc ttgggtgatt ttcgtaccag cacaatcagt ctgccctagt tgacagatcg
     1501 cgccacacta gccagataca gcgtatcatt ggtaaaactg ataatgcggc aatataaaga
     1561 aaaacttcaa tagtatgtta acgaaatttg acgcataaaa atacgctaaa aaattttcgc
     1621 ttgactaatt aaaaatggga ttgagcataa aattttaata atttattata ttattgaaaa
     1681 tatctcagat tttcagtcag tttattttat aagccgaacc gtaggctatt ttccatcttt
     1741 aaccatgaat agacctcaac ttcatcataa tttgtgcctg caatagcaac ttccagaaag
     1801 tcaataattc tacctgcttt attcaaagac tctgtagtat atagcaggaa agtcgctgca
     1861 tctgacttat ccggtgcagt gatgtctcct tcaaaaaaaa tagggatatt ttcgtattta
     1921 tttagattag ttctttctaa atttacctga aaatcaatgt agcacccaca tcctgtatta
     1981 tctctagata ctattattga attttctatt tgctctatta aatattcctt ccatatcata
     2041 ttgttttcaa taattctaca taagaaagcc ttctcatatt cagataaaca aaactggttc
     2101 atatattcta cccttacatt ttcaaaatag ttgcaatacg agatactcca ttgttgacat
     2161 atacgcgaac aattaccgtt ataccattga ccgttacagt aactggataa tttcctgttt
     2221 ttgtcagttt taaagcatcc actgcctttt gcgtcgctac aacagcattt cccatggagc
     2281 ctgatttttg aatcaatgta ttaccgatgc ttccttgatg ccccttaaag atgtgatgaa
     2341 ctttatttag ccaatctaca ccgtatcttt cagccaatag tcgagaatta ctttcaccta
     2401 ttttttccat cgaagctaaa atcctttgag cataggcagc atcagctgtt gaaaaatttg
     2461 cttcgcccaa caaaagccgt aattcctgaa tatctcctcg taaaattgca tttcttactc
     2521 ccgcttctaa tgtggtttgt ggctgaaaga gggaattatt ctctaccgcc acagtagccg
     2581 cattcgccgc tgtattcaca tccccgccta caacggcaga cgcagtgccg gcaaccagtt
     2641 ttgaataggc agtaatcttc gctttagctt tttcgatttc ggtcgcactc atacgactga
     2701 aatcagtatt cttaaccaaa gcctcaccaa caatctcacc gactgcagcg cctatcgcac
     2761 catcctgaca cttaccctta ttcgccgtcg ctgctgcaca gcctgctacg gcatgggcaa
     2821 tcttgtgggt ggtatatcgc tgccagccct ctctgttgat aatcttgcgt atgattacgc
     2881 cgtttgatgg ctaatcgtac ctacgcaggc ttaaaatttc catcaatcca tatcatttga
     2941 gttttcatct tcataatatg gatgatcaaa caaatctcct tcccaaatac attcactcgt
     3001 tataccttgg gattccatga aaggaacata agaataaaaa tctttttcat attctccata
     3061 aaatatattc atacctttga gccatactgc atcgcattca tcacacagaa taatttcatc
     3121 atgaagatat ttaggtttcg aatggtagag atagccttgt tgacaagcag gacacatttt
     3181 cattctctta tttatgatca ttccatataa tcaatttatt tttgtgggta agcaatagtg
     3241 atttgattgc tgcctttagt ctgtacaaca atacgtatat agcaatctga taatttcctg
     3301 gtttttatca gctttatcct cagattccaa acccaataat aaaagccgtt tgaaattttt
     3361 ttcagacggc cttaaaactt aattaaataa tgtccggatt taacaccaaa tcatattcac
     3421 caaactttgg aaatagaatt accttatctt tttggggata taattctgtt ttttcatatc
     3481 gctcattatt aataaaatct tgtttcaaaa catagcaatc atccgtattt tcattgaaga
     3541 aataagtata tccttctgct aagtcatgag attccaaagt tatataatct ctccatacag
     3601 ccatagaaag aaaataataa ttcgaattgg aatatttttg aaattgatgg gttaaataat
     3661 cccttccaaa tttatcgaaa aaatgtaccg aaaaaaaacc tacttcctct cgcaaggtaa
     3721 ttacatattc aggttgtgtc gccgaaccta ttacggcagt atataactcc cctttaagat
     3781 gtttttcctc agcttgtttc tcagaaaaag gcttatgaag tttcttataa ccataagacc
     3841 actgctcaca ataaaaaata tctttcttca tttgtaaatc cttcctgttg tatctctaat
     3901 gataacattt tcttcttgtg ccgcccttaa tacagattgt ggaattggat tgacctgagc
     3961 aggaacttct aaaataagtt ttccttcaag ctttcttcct cttatcgtag taggtgtact
     4021 tggaacattg gaaatgacag caccagggct atattttcta acagcctcct tgatataacc
     4081 aatccccgta ctttcttgga tttgagaaaa ttgggtaaat tttcttgaaa taatttcctc
     4141 tgtcgcagga ttatacgaat ctaccctaaa atgtcccgtt accgatttag gattggctac
     4201 ataaagctca ttataagggt attttgatga catgatacgg ttaaattcat tgccgttgtt
     4261 tatcctcatt ctataaattg ggagaacagg aatgcctctg gattccctta attgattata
     4321 atattgcctg tatgtttgta catcatgtct tgtccacggc tctctaggat tcctcataat
     4381 agcaatcccg ttaaatttcg gatccagcct tcggatttga ttggtaatgg cctggatttc
     4441 aagaatggca tattcatgtt ccaattcttg tcgcggagtc caacgtatat ttaccctcct
     4501 gcgagctaaa agactattat tctccactgc cacagtagcc gcattcgccg ccgtattcac
     4561 atccccttta accaatgcca ctgcgctgcc tgcgataatc tgcgagtagg ctatgacttt
     4621 ttggcgttct tggggtgaca gtttgcctac atcgtgtccg tccaacaggg tttctcccac
     4681 catctcgccg actgccgcgc cgattgcgcc gtcccgacat ttgcctttat ttgctaccgc
     4741 tgccgcacag cccgctatgg catgggcaat cttgtgggca atgtagtctt cgctgagatt
     4801 aaatttgatt ttgctcgcta cttctccgtg tacggtactg actatcgcac ccagtgcggc
     4861 atcgcccaaa ttgtctttca ggctgccgcc gttgatggcg gtatggacac ttgcggcagc
     4921 ggtgctgttg atcaggttag cggtcagttt gcctgctgcg ggactgtgaa aatgtttgct
     4981 gacggcttcg gctgcttggg tgttcagccc gcttatgccc tgcagtacgc ctgcggttac
     5041 ggcggcggtg gcggcctgtc tgacggtgct gcttttgccc agttctttca gggtatggtt
     5101 tatgtctcct ttgttgttga tgagggaaac tgcggcttgg ctggcgagac tggccagtgc
     5161 ggctttgcct gcggctgtgg tgatagctgc agctgatgtg cctgctgcta ctccggttgt
     5221 ggctgccgtt cctgctgcta ctccagttcc ggctgccgtt cctgctgcgg ctcctcccgc
     5281 tcctgcgcct gccgcgcccg ccgttccggc ggctgccggg gcggacagtg caccgtaggt
     5341 caatacggtt acgacgataa cgacgacagc tgctgctgcg ggtgtcatgc cctcctgttt
//
LOCUS       DAWKOG010000139         5389 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.139, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000139 DAWKOG010000000
VERSION     DAWKOG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5389
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.139"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(99..2933)
                     /gene="lbpA"
                     /locus_tag="WJK66_001716"
                     /gene_synonym="tbpA"
     CDS             complement(99..2933)
                     /gene="lbpA"
                     /locus_tag="WJK66_001716"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HEZ7579387.1"
                     /translation="MNKKHGFQLTLTALAIATAFPAYAAQAGAAAPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVAYGINRLDAFRQTHDIQSQDKTGQFFLVEGENTL
                     KPVAKLAGYGIYLNRQLNRWVKERIEKNQPLSAEEEAMVREAQARHENLSAQAYTGGG
                     RILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEADKF
                     RDKSGVYDGDDFRDGLYFVPNIEEWKGDKKLVRGIGLKYSRTKFIDEHHRRRRMGLLY
                     RYENEAYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSS
                     DRFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASNAISRPEQLSHNAARISEST
                     GFDEKNQDKYRLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYF
                     DFSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSLSYRASSGFRTPSFQ
                     ELFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVAD
                     HKTKLPNQAGQLTEIDIRDYYNAQNMSLQGVNILGKIDWNGVYGKLPEGLYTTLAYNR
                     IKPKSVSNRPGLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELA
                     YLAGDQKRYSTKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAE
                     STANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(2930..5137)
                     /locus_tag="WJK66_001717"
     CDS             complement(2930..5137)
                     /locus_tag="WJK66_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ7579388.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPVTFKSK
                     DVPTSPPAGPSVETTPVNRPAVGAAMRLLRRNIATSDKDGNDFPNSRQAEEKLSFKEG
                     DVLFLYGSKKDQRQQLEDEIRQRNPNVQIRTSENENKKYGYNFVDAGYVYVKGEDEIE
                     RTSNYKYFTHRFGYDGFVYYSGEHPSQSLPSAGTVKYFGNWQYMTDAIRHRTGKAGDP
                     SEDLGYLVYYGQNVGATSYAATADDREGEGKHPAEYTVDFDKKILEGKLIKNQYVQRK
                     TDQKKPLTIYNITAKLDGNRFTGSASVNPDLAKNHANKEHLFFHTNADQRLEGGFFGD
                     NGEELAGRFISNDKSVFGVFAGKQKTETANASDTNPALPSGKHTKILDSLKISVDEAS
                     DKNPREFAISSMPDFGHPDKLLVEGREISLVKDTQTIDLADGRKMTVSACCDFLTYVK
                     LGRIKTDRPAAKPKAQDEEDSGINNGEESEDEDEVVEEEADEAEADEAEDEEAEEIEE
                     EAEEEETEEKSPAEGNGGSGSILPAPEAPKGRDIDLFLKGIRTAETNIPQTGKAHYTG
                     TWEARIGTPIQWDNQADKEAAKAKFTVDFGGKSISGTLTEENGVEPAFYIEKGVIDGN
                     GFHATAHTRDTGINLSGTGSTNPQRFKASNLRVEGGFYGPQAEELGGIIFNNDGKSLG
                     ITEGTENKVDVDAEVDAEVDAEVDVDVEQLKPEAKPQFGVVFGAKKDNKEVEK"
BASE COUNT         1180 a         1439 c         1234 g         1536 t
ORIGIN      
        1 atcttgttta tatcgaatca aaaaaacctg caccttaatc agttggcggt ttagtccgac
       61 ttttggggtg cagatcaagc tttcagacgg tatttccttt aaaacttcat ttcgagcgcg
      121 aggctgaagt tcctgcccgg tgcggcatac cttccatagt tgctgtcgcc gccgtgccgg
      181 tttgccgtgc tttccgcggt ctggcgcaag gattcccaag taacgtagcg gtagttgccg
      241 atattgtaga tagccgccct caaggtcagc cgttttttca gattcagata ggcggaaacg
      301 tctgccgtcg accaagaaga cgacgctctt tttgtcgaat atcgtttttg atcgcctgcc
      361 agataagcaa gctcgtcagg gtttttccct ttggaatagg tcagcataat gtttgcgccc
      421 catttcccct ctggctggtc gtatccgaac cccaaaacat aacgcgacgg ctgtaccgca
      481 tccaaagcat agctgcggag ggacagtccc ggccggttgg ataccgattt cggtttgatg
      541 cggttgtacg ccaaagtcgt gtacaaacct tcgggcagtt tgccatacac gccgttccag
      601 tcgatttttc ccaatatatt aacgccttga agcgacatat tttgggcatt gtaataatcg
      661 cgtatatcaa tctctgtcaa ttgtcctgcc tgattcggca atttggtttt gtgatcggca
      721 acggcaatca tatcggtata acggttgcgg aagctgctga tttccaaaaa gccgaaatcg
      781 cccttccact gcaaaccgat ttcgcggttg gctgcctttt ccgatttcag ggcgggacgc
      841 tgccagcctt tcggataatc gtgataaatg tctatcccga aaagttcttg gaatgagggc
      901 gttctgaagc cgctggaggc gcggtaagac aaagaaaaat gccggttcgg tttgaacacg
      961 atgccgctgt tccacgaacg gtcaacatac cgcccgctgc ggacgagttc ttccgacgtg
     1021 gtgaagtttt tccggtcata cctgccaccc aagctgaaat caaaatattt gccgattgaa
     1081 aaacggtcgt tcaaagaaat atggatattg ctgccgttga tttttcttgg cacgcatttg
     1141 cgggaacgca gggtttcgat gtagccgcag accgaccctt cgacgacttc gggcttaccc
     1201 aaacggtact tatcttgatt cttttcatcg aatcccgtgg attccgaaat ccttgccgca
     1261 ttgtgggaaa gctgttcagg gcgggagatc gcgttggaag catcgtaacc gaagcccaaa
     1321 gtcagatggt gtttcgtcca tttgtttttc agcgacttct caaacgaggc attcaaaaca
     1381 ttgtgctgtt cgcggtagtg gaaacggtcg ctgctgtcgt aggaatacgg tttgtccgcc
     1441 gacgcgcggc aggatttgtc cacagcagga tacacggcgc aattcagctt cagcgtgttg
     1501 ttgtcggttg ccacgccctg tttgtcaaac gacaacaccg ccttatccgc ccagttgtca
     1561 gaatacgctt cgttttcata acggtacagc aaacccatac ggcggcggcg gtgatgttca
     1621 tcaataaatt tggtgcggga atatttcaaa cctatgcccc tgaccaactt tttatcgccc
     1681 ttccactctt ctatattcgg cacaaaatac aagccgtcgc ggaaatcgtc gccgtcgtac
     1741 accccgctct tgtctctaaa cttgtccgcc tcgtccgtac cgtaatactg tttttccgtc
     1801 atatcgcgga tatcgtaacg ctgtttggta tcctcaaaca cgccgccgac ataatgcctg
     1861 ccgccgaagc ggtagcccag cttggcaagc caagagccgc tgcggtaatc catcggatcg
     1921 ggcaatatcc tgccgccgcc cgtgtaagct tgggcggaca ggttttcgtg gcgcgcctgc
     1981 gcctcccgca ccatcgcttc ttcttcagca cttaaaggct gatttttttc aatacgttct
     2041 tttacccagc ggttaagctg gcggttcaaa tatatcccgt agcccgccaa ttttgccacg
     2101 ggcttgagtg tattctcgcc ctctactaag aaaaattgcc ctgttttatc ttggctttga
     2161 atatcgtgtg tctggcggaa cgcgtccaaa cggtttatgc cgtatgccac cccgtccgca
     2221 atatcgccgt gcgggcgcgt ttcccgccct tggcgttcgg ttcggattaa cagcccttcc
     2281 caaccgtctt tgctgaaccc cgcgccgagc gacttcataa attggcggtt tttactgccg
     2341 taggcggttt ttgcctgtat cccccagctt ttgccgtctg aaatcaggtc tgccgcttct
     2401 ttggtgcgga aggcgaccgc gccgccgagt gcgccgctgc cgtgatcgga cgaaccggca
     2461 cctttgtcga tttccaccgt gctgatgttt tcatattcga tttcgttgat tgcgccgctg
     2521 ccgccgcgtc cgccgtatcc gctcaacgat ccctgcacgg taaacgcctg tatttgggca
     2581 acgccgtcaa ccgaaaccgc cacacggttt ttatccacgc cgcgtatcga gtagccgccg
     2641 ctcgcgccgt tgccctgttc gacaaccgcc acgcccggat cgtagcgcgt caggtcgcgg
     2701 ataccgagta cctgttcttt gttcaacgtt tccgacgttt tgacgatttt gcccaaaccg
     2761 gtcgcctctt tcgatcgccg tcccactttg gcggcacgga cggtaacctc tttcagggat
     2821 tgggtctgcg cggcatcagg tgccgccgcc cccgcttggg cggcataagc cggaaaagcg
     2881 gttgcaatgg ccaaggcggt cagggtaagc tgaaaaccgt gtttcttatt catttttcca
     2941 cctctttatt atctttcttc gcaccgaata ccacgccgaa ttggggttta gcttcaggtt
     3001 ttaactgttc aacatcaaca tcaacttcag catcaacttc agcatcaact tcagcatcaa
     3061 catcaacttt attttcagta ccttcagtta taccaagaga tttcccatca ttattgaaaa
     3121 taataccgcc caattcctcc gcctgcgggc cgtaaaatcc tccttctaca cgaagattac
     3181 tggctttgaa acgttgagga ttagtcgaac cagttcccga aagattgatg ccggtatccc
     3241 gagtgtgtgc tgtcgcgtgg aaaccgttgc catcaatcac acctttttca atatagaaag
     3301 caggttctac accgttttcc tccgtcagcg ttccagaaat cgatttcccg ccgaaatcaa
     3361 cggtaaattt tgcttttgcc gcttctttat ccgcctgatt gtcccattga atgggtgtgc
     3421 cgatacgcgc ttcccaagtg ccggtatagt gtgcttttcc agtttgtgga atattcgttt
     3481 ctgccgtgcg gatacctttc aggaaaaggt cgatgtccct gcctttaggg gcttccggag
     3541 cgggcaggat gctgcctgaa ccgccgttgc cttctgccgg cgatttttct tcggtttctt
     3601 cctcttcagc ttcttcttcg atttcttcag cttcctcatc ttcagcttca tcagcttcag
     3661 cttcatcagc ttcttcttcg acgacttcgt cttcgtcttc gctttcttca ccgttattaa
     3721 tacccgaatc ctcttcatcc tgcgccttcg gtttggcggc gggacgatcg gtttttatcc
     3781 gtccgagttt cacataggtc aaaaagtcgc aacaagcact gacggtcatt ttcctgccgt
     3841 cggcaagatc aatggtttga gtatctttga ccaaggagat ttcacgccct tcgacaagaa
     3901 gcttgtcggg atgaccaaaa tcgggcatag aggaaatggc aaactcacgg ggatttttat
     3961 cacttgcctc gtcaacggaa atttttagag aatccaagat tttggtgtgt tttccagacg
     4021 gcagggcagg atttgtatct gatgcgtttg ctgtctctgt tttttgtttg cctgcgaata
     4081 cgccgaatac gcttttgtcg ttgctgataa atcgtccggc aagctcttct ccgttatcgc
     4141 cgaaaaaacc gccctcaagc cgctgatcgg cattggtatg gaaaaacaaa tgctccttat
     4201 tggcatggtt tttcgctaaa tcaggattga cactagcact gccggtaaag cggttgccgt
     4261 ccaattttgc agtaatgttg taaatggtca gtggtttctt ttgatcggtt ttcctttgca
     4321 catactgatt tttaatcagc ttgccctcca ggattttttt gtcgaaatca accgtatatt
     4381 cggcaggatg ttttccctcc ccctcccggt cgtcggcagt cgcagcataa gaagttgctc
     4441 cgacattttg accgtaataa acgagataac ccaaatcttc gctaggatct cctgcttttc
     4501 ctgtccgatg acgtatggca tcggtcatat attgccagtt accgaaatat tttaccgttc
     4561 ccgcgctcgg taaagattgc gaaggatgtt ctccggaata atatacaaaa ccatcataac
     4621 caaaccggtg agtaaaatac ttgtaatttg aagtcctctc aatttcatct tctcccttta
     4681 catatacata acctgcatct acaaagttat aaccatattt tttattttca ttttctgatg
     4741 tcctaatttg tacattagga ttgcgttgac gaatttcatc ttcaagctgc tgacgttgat
     4801 cttttttgga accgtataaa aacagaacat caccttcttt aaacgacagc ttttcttctg
     4861 cttgtctgct atttggaaaa tcattgccat ccttatcaga agttgcgata ttccgcctca
     4921 acagccgcat tgccgcaccg acggcgggcc ggttgaccgg cgtggtttct accgaaggcc
     4981 cggcaggggg cgaagtggga acgtccttag acttgaaagt gacggggtac gcggtcggcg
     5041 ttgattcgac aacaggctgc acgccgaaat tgccgccgat acaagatgcc aaaagtaagg
     5101 gcaacaagac aatgccgcca taattcggtt tacacatccc tacttttcct ctatttgatt
     5161 aataataatt atcattatat taatatgtac agataatatc aagccgtttt tatagtgaat
     5221 taacaaaaat caggacaagg cgacgagccg cagacagtac agatacatcc cgtcattccc
     5281 acgaacctac atcccgtcat tcccacgaac ctgcaccacg tcattcccac gaaagtggga
     5341 atccagttcg ttcggtttcg cttgttttaa gtttcgggta acttctact
//
LOCUS       DAWKOG010000140         5360 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.140, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000140 DAWKOG010000000
VERSION     DAWKOG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.140"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(83..1129)
                     /gene="recA"
                     /locus_tag="WJK66_001718"
     CDS             complement(83..1129)
                     /gene="recA"
                     /locus_tag="WJK66_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEZ7579389.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEIADEIDAKIRALNGIEMHIT
                     EGTQDETDGERPEE"
     gene            1383..2147
                     /locus_tag="WJK66_001719"
     CDS             1383..2147
                     /locus_tag="WJK66_001719"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEZ7579390.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAELSAVLEQIKNMPFD
                     IAEFRADFLECAGSIGEILHHTQTVRDALPDKPLLFTFRRHGEGGSFPCSDDYYFKLL
                     DALIESRLPDIIDIELFSGETAVRCAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLKAKELAAKPIVTMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRATLDCLENGAD"
     gene            2167..4182
                     /gene="rep"
                     /locus_tag="WJK66_001720"
     CDS             2167..4182
                     /gene="rep"
                     /locus_tag="WJK66_001720"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HEZ7579391.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKPQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNIWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSKLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGSYRAKAETGEAGEFINSLLEEIDYENHLLASEEGKAGEIKWRNVGDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGTWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDNLKKSGAAD"
     gene            complement(4250..5308)
                     /gene="dinB"
                     /locus_tag="WJK66_001721"
     CDS             complement(4250..5308)
                     /gene="dinB"
                     /locus_tag="WJK66_001721"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEZ7579392.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYASEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLVRGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
BASE COUNT         1238 a         1476 c         1482 g         1164 t
ORIGIN      
        1 aaactcaaat caagccgttc ggaggctgct caaaaaaaac ggtacttcgc aacagaagta
       61 ccgtttatcg ggatttcagg ttttattctt cggggcgttc gccgtcggtt tcgtcctgcg
      121 tcccttcggt gatgtgcatt tctatgccgt tgagggcgcg gatttttgcg tcgatttcgt
      181 cggcaatttc gggattttcc ttcagccaga cgcggacgtt gtctttgccc tgaccgattt
      241 tcgcgccgtt gtagctgtac cacgcgccgg atttgttgat gatgtcgttt ttcacgccga
      301 tgtcgatcaa ttcgccttcc caactgatgc cttctccgta gaggatgtca aactctgcct
      361 gacggaacgg cggcgcgact ttgtttttga tgactttgac gcgggtttcg ttgcccaata
      421 cttcttcacc ttttttgatg gatccggtgc ggcggatgtc gaggcggacg gaagaataga
      481 atttcagcgc gttgccgccg gtcgtggtct cggggctgcc gaacattacg ccgatcttca
      541 tccggatttg gttgatgaac acaaccagcg tgttggtttt tttgatgtgt ccggtcagtt
      601 tgcgcaaagc ctggctcatc aggcgcgcct gcagtccgac atggctgtcc cccatatcgc
      661 cttcgatttc ggctttgggg acgagtgcgg ctacggaatc gacgactacc atatctatgc
      721 cgcccgaacg gacgagtgtg tcgcagattt ccaaagcctg ttcgccggta tcgggctggg
      781 acaggtaaag ctcttcgact tttacgccga gtttgcgggc gtaaacggga tcgaaggcgt
      841 gttcggcatc gacaaaggcg cacacgccgc cgtttttctg gcattgggcg acggcttcga
      901 ggcagagggt ggttttgccg gaggattctg ggccgaagat ttcgacgatg cgcccgcgcg
      961 gcagaccgcc gactccgagg gcgaggtcta atccgagcga tccggtggaa atgacttcga
     1021 ggttttcttc ctgctggctg ccgtccattt tcatgatggc gcctttgccg aaactttttt
     1081 cgatttgcgc cagtgcggcg gcaagtgctt tgcttttgtc gtctgacatt ggggttactc
     1141 cggaacaaat gcggtatgtg ggatgcggcg caacacgggc tgcggcgcgg gatgtgtatc
     1201 gttttcccga tgtgcgggct atcggtaatg ctgcttcacg aggttgccat tatcgcatat
     1261 ttccttgctt gccgatatgc gccaggacgc ggcggcttgt gccggaatgg aatctggatg
     1321 ccgtctaaaa ggcggccggc tttgttataa tggcggctgt tttttctgtg tgtgcctgtt
     1381 ttatgtgttc ctgccttgtt gtcaaaaata ccgttatcgg aagcggacgc accaaaatcg
     1441 ccgtgccgct tgtcgcccgc gatgccgccg aactttccgc cgtacttgag caaatcaaaa
     1501 atatgccctt cgatattgcg gagttccgcg ccgacttttt ggaatgcgcg ggcagtatcg
     1561 gcgaaatatt gcaccacacg cagaccgtcc gcgacgcgct gcccgacaag ccgctgctgt
     1621 ttacgttcag acggcatggc gaaggcggct cgttcccgtg ttcggacgat tattatttta
     1681 aactgctcga cgcgctgatc gaaagtcgcc tgcccgacat catcgacatc gagctgtttt
     1741 ccggcgaaac cgccgtccgg tgcgccgtgg caaatgctca aaaaaacggc atcgccgccc
     1801 tgctctgcaa tcatgagttt caccgcacgc cgccgcaaga agaaatcgta tgccgtctga
     1861 aacagatgga ggactgcggc gcggacatct gcaaaattgc ggtgatgccg caaagcgcgg
     1921 aagatgtgct gactttgctt tccgccacgc tcaaagcgaa agagcttgcc gccaaaccga
     1981 ttgttacgat gtcgatgggg cagacggggg cggtcagccg gcttgccgga caggtgttcg
     2041 gctcaagcat cacgttcggt tcgggaacgc aaaactccgc gccggggcaa atcggcgtat
     2101 ccgccctccg tgcgacactc gactgcctcg aaaacggagc agactgattt cagacagcat
     2161 caaaacatga tgaaactcaa tccccaacag ctcgaagccg tccgctacct cggcggccca
     2221 ctgctcgtcc ttgccggtgc aggcagcggc aaaaccggcg tgattactca aaaaattaag
     2281 catttgattg tcaatgtcgg ctacctgccg cataccgttg ccgcaattac ctttaccaac
     2341 aaagccgctg cggaaatgca ggagcgcgtt gccaaaatgc tgcccaaacc gcaaacgcgc
     2401 gggctgacga tttgcacgtt ccactctttg ggcatgaaga ttctgcgcga agaggcgaac
     2461 catatcggtt acaaaaaaaa cttctccatt ctcgattcta ccgacagcgc gaaaatcatc
     2521 ggcgaactct taggcggtac gggcaaagaa gccgtattca aggcgcagca ccagatttcc
     2581 ttgtggaaaa acgatttaaa aacgcctgaa gatgtcgttc agacggcatc gaacatttgg
     2641 gaacaacaaa ccgcacgcgt gtatgcgagc tatcaggaaa ccttacaaag ctatcaggca
     2701 gtggacttcg acgacttgat ccgcctgcct gccgtgctgt tgcagcaaaa cagcgaagtg
     2761 cgcaacaaat ggcagcggcg gctgcgttat ctgctggttg acgaatgcca agatacgaat
     2821 acctgccaat ttacgttgat gaaacttctg accggcgcgg aaggtatgtt taccgccgtc
     2881 ggcgacgacg accagtccat ctacgcatgg cgcggcgcga acatggaaaa cctgcgcaaa
     2941 atgcaggaaa actatccgca gatgaaggtc atcaaactgg agcaaaacta ccgctccacc
     3001 gcgcggattc tcaaaatcgc caacaaagtc atcgagaaca accccaagct gttcaccaaa
     3061 aaactttggt cgaaattggg cgaaggcgag cccgtcaaag tcgttgcctg ccaaaacgag
     3121 cagcacgaag ccgactgggt cgtcagccaa atcgtcaaac aaaaactcat cggcggcgac
     3181 aaaacccaat atgccgattt cgccgtgtta taccgcggaa agcatcaggc gaggattttc
     3241 gaggaagcat tgcgcggcgc gcgcatcccc taccgtctct ccggcggaca aagctttttc
     3301 gacaaagccg aaatcaaaga cgtgttgtct tatgtgcggc tgcttgccaa ccccaacgac
     3361 gatcccgcct ttctgcgtgc cgttaccacg cccaaacgcg gcatcggcga tgtaacgctg
     3421 ggcaagctca acacttacgc gcacgaacac gaatgcagcc tgtatgaagc cgcgcaaaac
     3481 gaagaagccc tcgccacgct gaacaatacc aaccgccaac acctgcaagc ctttatggat
     3541 atgttcggca gttaccgtgc caaagccgaa accggcgaag cgggcgagtt catcaacagc
     3601 ctgctcgaag aaatcgacta tgaaaaccac ctgcttgcca gtgaagaagg caaagctggc
     3661 gaaatcaaat ggcgcaacgt cggcgatttg gtatcatggt ttgcgcgaaa aggcggggaa
     3721 gacggcaaaa acatcatcga actcgcccaa accgtcgcct tgatgacgct tttggaagga
     3781 aaagacgaag aagaaaccga tgccgtctcg ctatccacgc tacacgccgc caaaggtttg
     3841 gagtatccgt atgttttcct tgtcggttgc gaagaaggcg ttttgccgca caacgacagc
     3901 atcgaagaag gcaacgtcga agaagaacgc cgcctgatgt acgtcggcat cacccgcgcc
     3961 aaacgccaac tcacactgac ccactgcgtc aaacgcaaaa aacaaggcac atggcagttc
     4021 cccgaaccca gccgattcat agacgaaatg ccgcaggaag atttgaaaat cctgggtcgc
     4081 aaaggcggcg agccgattgt cagcaaagaa gaaggcagac gcaaccttgc cgatataatc
     4141 ggaaggctcg acaacctaaa aaaaagcggc gcggcggatt aaaccggagc cgcaatgccg
     4201 tctgaaggct tcagacggca tattttttgg acggcgcgcg taaagcggtt tacgcccaca
     4261 aatcctgctg ctggtttttc ggcacaagat gccccacgcc gataccgata aggcggaacg
     4321 cgtcttccgt ctgcggcggg acgcgcgcca tcaacatttg cgcagcctgc agcagagctg
     4381 cgcagtcggg caatacggag gaataagtca gtgtgcgcgt gatgatgcgg aaatcgtagg
     4441 tcttcagctt gagcgttacg ctttgggctt cgacgttttt gcgcgtgatt tgccgccaca
     4501 agtcttcggc aagatggggg aggtgtccgg cagcctgctc gagcggcagg tcttcgggca
     4561 gggtaatttc tgtggagatt tggaggcgtt cgcgttcggc tttgacgggg cgttcgtccg
     4621 taccgcgcac caaatcatag aggcggtatc cgtagcgtcc gaaatggttt aagagttcgc
     4681 cgcgctcgaa acggcgcaag tcgcccgccg tccgcatacc cagcgactgc atttttttca
     4741 gcgttacctt gcccacgccg gggattttgc ccaaaggcag ggtttccaaa aatgccatga
     4801 ctttgtgcgg cggcaacaca aactgcccgt tcggcttgcg ccagtccgac gcgattttcg
     4861 ccagaaattt gttcggcgcg atgcctgcgg atgcagtcaa acctgtttcc gcaaaaatgg
     4921 cggcacggat ttctttggca acttcactgg cgtaagggat gtttttgaaa ttacgggtaa
     4981 cgtcaagata ggcttcgtcc agcgacaagg gttcgattaa atcggtataa cgcctgaata
     5041 cggcgtgaat ctgcgcggaa acctgacggt acaaatcgaa atgcggcggc acatacaccg
     5101 cttgcggaca cagccttttc gccgttgcca ccgacatcgc ggaatgcagc ccgaactgcc
     5161 gtgcctcata cgatgcggcg caaatcaccg aacgcgcgcc ctcccacgcg acgaccaccg
     5221 gccgcccttt caaatgcggc tgttcgcgca gctctaccga tgcgtagaat gcgtccatgt
     5281 cgatgtggat gattttgcgt gaagacatcg gctctgctga ggataaaaag gatattctac
     5341 tgccggcatc gggcaaattc
//
LOCUS       DAWKOG010000141         5350 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.141, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000141 DAWKOG010000000
VERSION     DAWKOG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.141"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            56..338
                     /locus_tag="WJK66_001722"
                     /pseudo
     CDS             56..338
                     /locus_tag="WJK66_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(359..1585)
                     /locus_tag="WJK66_001723"
     CDS             complement(359..1585)
                     /locus_tag="WJK66_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEZ7579393.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RHGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMMPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(1600..2025)
                     /locus_tag="WJK66_001724"
     CDS             complement(1600..2025)
                     /locus_tag="WJK66_001724"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579394.1"
                     /translation="MPSEHAAYADLTASSAKRLLCAKAILFSSAVRTGAAVGNIFFDG
                     FADGGDFGDGAQYGFATFNAVDAASDSECGIAGAAVLEIVEDEDFEGDLGSWADSVKM
                     VRSSDGITVVPMPSETAAVQAFVPDGKIGYNIGHQCPTY"
     gene            2130..2792
                     /gene="queC"
                     /locus_tag="WJK66_001725"
     CDS             2130..2792
                     /gene="queC"
                     /locus_tag="WJK66_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEZ7579395.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQTYRRENVQAITFRYGQRHA
                     VELERAEWIAQDLGVSQTVLDLSLMRQITHNALMDEAAAIETAGNGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGALDYIREQTHTCYNGIVGGGCRECPSCILRERGLAEYLESKK
                     AV"
     gene            2820..3338
                     /locus_tag="WJK66_001726"
     CDS             2820..3338
                     /locus_tag="WJK66_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HEZ7579396.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DADKIHIDGWQIVEWADGYDIAVSETPKTKMPSEHAPRLYFANVGGYRAGQLAEAYAF
                     GLFAAATPAEAKQKALQTLLTDSYVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGK
                     PAEIGFQGYLPI"
     gene            3345..3767
                     /gene="queD"
                     /locus_tag="WJK66_001727"
     CDS             3345..3767
                     /gene="queD"
                     /locus_tag="WJK66_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEZ7579397.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDDPVRGG
                     PKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNGRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            4036..4407
                     /locus_tag="WJK66_001728"
     CDS             4036..4407
                     /locus_tag="WJK66_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEZ7579398.1"
                     /translation="MKLLFIPLVLFVAVEHFYIAWLEMTQIPSEKAAETFKLPYEFME
                     QNRVQTLFGNQGLYNGFLGIGLVWSRFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            4404..5042
                     /locus_tag="WJK66_001729"
     CDS             4404..5042
                     /locus_tag="WJK66_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEZ7579399.1"
                     /translation="MKKISVAPENPQYRIVEIFESLQGEGWNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGRLKAYAARNIIITGGEPTIQPHLDMLLDMLKAEGYFL
                     CIETNGLNPAPPQIDYVATSPKACYAAKYEKSCIETADEVRIVADGDVVAFCENMERK
                     IRAHHYYLSPCEQDGAMNIYDTIRQIGILNSRPDAPVHWQLSVQTHKWAGIE"
BASE COUNT         1379 a         1535 c         1333 g         1103 t
ORIGIN      
        1 ttttttcgat acggaaccgg atagaggaaa aatcgaacat tgcgcctgcc ttgctatgat
       61 tcacgaagaa atctccgcca tgcctatggg ctatgaaacc ttgatcggcg atatgggcag
      121 cgcactgtca ggcggacaaa aacaacgcat cgtattggcg cgggccttaa tattgcgaac
      181 cgaaaatcct atttttagat gcagcgacca gccatttgga tattgccaat gaaaaagcag
      241 tcaatgcaaa cttgaatggc ttgtctatca taaaaattat ggcggcacac agaaaggaaa
      301 cggtggaatc agcagatagg aaaatgtctt taggataaaa atacagtttc aaaaatactc
      361 aagattgctg ctgttttttc gcctgagcgt caaactctgc cagcgtcatg ttcaaagtct
      421 gcaaacacgg catcattact gcatcgacag cttggttcac atgatccctt tccacaggca
      481 acggacggta aacgaagagc ttgaagagtt cgttcaactc aatcgaatcc gcccccgttt
      541 tcaacaccca accctgtctg ccggaataga tgtagccgtg ccgcgccaac ttttccaaga
      601 gttcgcccaa ctcgtcgtag cccatattga tatgccgtct gaactcctga acaggcaagg
      661 ctttgccttc tttttgcgcc gcatccagaa gcagcaggat tttcaacacg tcgtcaaacc
      721 gtccgcgcga gtcgaagccc ctgcggaacg cttctccctg ccagtaggag agtgaagaag
      781 tcagcaccgc gccgcccaag accagcgtcc acaacaggtt cagccacaac agaaaaaacg
      841 gcacggcggc aaacgcgccg taaatcgagc ggtagccgtc gaagttgccc atataccaag
      901 tgaagaggga acgtgcggtt tccagacaaa acgctgttgc caaagccccg acaaacgcct
      961 gccgcgcggg aacgaagcgg tttggcacga agcggtacag cccccacagc aaaagcgtca
     1021 tgaaggtcag cgtcgccgcc gttcgcaacg cgcccgacca ctgcggcgca cctgaggcaa
     1081 gcgcggcatc ctgtaccgag ccgaccataa aggaaatgcc cacgcccaaa gacagcggcc
     1141 cgaacgtcag taaagcccaa tagacgagaa actgcatcat ccacggacgc tgggaattga
     1201 cccgccagat gcggttgaac gtattgtcta tcgtccgaat cagcatcagc gaggtaacga
     1261 ccagcatcac gctgccgatt gccgtcagcc ggttcgcctg ctcgcggaac gcattgatat
     1321 agtcgaacac catgtccgcg ccctgcggca caatggtttg gttgacgaag gagacgaacg
     1381 aatccgacca gcggtcgaac acggggaaaa tcgaagcgac cgccaccatc acggtcagca
     1441 cggggacgag tgccagcagc gtcgtaaacg tcatgcttgc cgccgcctgc ggtacgcgtt
     1501 cttcatcaaa gcggcggacg acgaaccatg caaacgcaca gattttattg tctgccaaac
     1561 cttgcaaacg ttgtaaaaag gtcataattt cttgcccggt cagtaagttg ggcattgatg
     1621 cccgatgtta tagccaattt tgccgtcagg aacaaatgcc tgaactgcgg ctgtttcaga
     1681 cggcatcgga acaactgtta tgccgtctga agaccgaacc attttaacgg aatccgccca
     1741 tgaacccaaa tccccctcaa aatcctcgtc ctctactatt tccaaaacgg cagcacccgc
     1801 aatcccgcac tccgaatcgc tcgcggcatc gacagcgttg aaggttgcga agccgtattg
     1861 cgcaccgtcc ccaaagtctc cgccgtctgc gaagccgtca aaaaagatat tcccgacagc
     1921 ggctcccgtc ctgaccgccg aagaaaacaa tatcgccttc gcacaaagca aacgcttggc
     1981 ggaactcgcc gtcaagtcgg cataagccgc gtgttcagac ggcatggcgt tcagatgccg
     2041 tctgaacgcg tttgcctgta taatccgcgt ctttactatc caacttcgcg gttcgcaaac
     2101 ctcccgcgtt accaaaacta ggattcgata tgtcaaacca aaaagccttg gtcatctttt
     2161 cgggcggcca agattccact acctgcctga ttcaggcaat ccaaacttac agacgcgaaa
     2221 acgtccaagc catcaccttc cgatacgggc aacgccatgc cgttgagctg gaacgcgcag
     2281 agtggatcgc gcaggatttg ggtgtcagtc aaaccgtact cgatttgagc ctgatgcggc
     2341 agattacgca caatgccctg atggacgaag ccgccgccat cgaaaccgcc ggcaacggtg
     2401 ttcccaatac ctttgtggac gggcgcaacg cgcttttcct gctttacgcc gcgatttacg
     2461 ccaaagggca ggggatacgg cacatcatcg cgggcgtgtg cgaaaccgac ttctccggct
     2521 atcccgactg ccgcgacgtg tttgtcaaat cgatgaacgt tacccttaat ttggcgatgg
     2581 actatgattt tcaaatccac acgccgctga tgtatctgac caaggcgcaa acttgggcgt
     2641 tggcggacga aatgggcgcg ctggactata tccgcgagca aacccacacc tgctataacg
     2701 gcatcgtcgg cggcggctgc cgcgaatgcc cgagctgtat cttgcgcgaa cgcgggctgg
     2761 cggaatatct ggaaagtaaa aaggccgtct gaacacgcgc aaaccataag gaatacgata
     2821 tgcccaagct ccatatgttt tacctcggcg gcaatgccgg caggtcgaat atcgaagtgc
     2881 acgacatcca atttgccgtg tgcgacgact accgtgaagc cgtccccgca ctcaaagccg
     2941 cgtggttcgg cgatgcggac aaaatccaca tcgacggctg gcagattgtc gaatgggcgg
     3001 acggttacga catcgccgta tccgaaacgc ccaaaacgaa aatgccgtct gaacacgccc
     3061 cgcgcctgta ttttgccaat gtcggcggtt atcgcgcagg gcagctcgcc gaagcgtacg
     3121 cttttgggct gttcgccgcc gccacgcctg ccgaagccaa acaaaaagcc ctgcaaaccc
     3181 tgttgaccga cagctatgtt cagcaacata aagacaactt aaaagacgtg gacaacctgc
     3241 ttgcgctcga ccgcatcggc aatttccata tccgcctgac cccgaatccg cacggcaaac
     3301 ccgccgaaat cggctttcaa ggctatttgc ccatttgaga acccatgaaa atcaccaaaa
     3361 tcttcacctt cgactcctcg catatgctcg acgggcatga cggcaaatgc caaaacctgc
     3421 acgggcatac ctacaaactc gaaatcaccg tttcagacga ccccgtcaga ggcgggccga
     3481 aagacggtat ggtgatggac tttaccgact tgaaagccat tgtcaaacaa cacattaccg
     3541 accccttcga ccacgccttc atctaccacg gcggcaacgg gcgcgaatct caaatcgccg
     3601 cactcttgga aggctggaac atgaaaaccc tgcgcctgcc ctgccgcacc actgccgaaa
     3661 atatggcggt cgaaatgtac gaccgtctga aaaacgcggg gctgaacgtg tgccgcgtga
     3721 aattgtggga aacgccgaca tcgtgtgcgg agtatgaagg ggagtaggga atatcttgaa
     3781 cgtatcgata tagtaaattc caataagaca tgcccaaccg cgtcattccc gcgcaggcgg
     3841 gaatccagac cttgatttat caggaatatt taaaaattgc agcaattcca aatctctgga
     3901 ttcccgcctg cgcggaaagg acggtttaga gcgtccttat ttgaatttac cgtaaaacgg
     3961 ttttttctcc tgtacggatt ccccgttttt tcagacgacc ttccatatca aatacaccca
     4021 ttaaaaggaa tatccatgaa actcctcttc atccccctag tcctcttcgt cgccgtcgaa
     4081 catttctaca tcgcctggct tgaaatgacg cagattccca gcgaaaaagc ggcggaaacg
     4141 ttcaagctgc cttatgaatt tatggaacaa aatcgcgtgc agaccctgtt cggcaaccaa
     4201 gggctgtata acggctttct cggcatcggg ctggtgtggt cgcggtttgc cgctccggat
     4261 aacgcggtgt acggcgcaac ggtactgttt ctcggcttcg tcctgattgc cgccgcgtgg
     4321 ggcgcgttct cttccggcaa caaaggcata ctcgtcaaac aaggtttgcc cgcatttttg
     4381 gcagcggcgg cggtgttggc ggtatgaaaa aaatcagcgt cgcccccgaa aatccccaat
     4441 accgcatcgt cgaaatcttt gaaagcctgc aaggcgaagg ctggaacacg ggcatgcccg
     4501 ccgttttcgt ccgcttgggc aaatgcaacc tcgcctgcgg ctggtgtgat accgattatt
     4561 tgacattcgg catgatgagc ctgtccgaca tcttaggccg tctgaaagcc tatgccgccc
     4621 gcaacatcat catcaccggc ggcgaaccga ccatccagcc gcatctcgat atgctgctgg
     4681 acatgctcaa ggcggaaggt tatttcctct gtatcgaaac caacggactc aatcccgcgc
     4741 cgccgcaaat cgactatgtc gccaccagcc ccaaagcctg ctatgccgcc aaatacgaaa
     4801 aaagctgtat cgaaacagcc gacgaagtgc ggattgttgc cgacggcgat gtcgttgcgt
     4861 tctgtgaaaa tatggaacgc aaaatccgcg cgcatcatta ctacctttcg ccctgcgaac
     4921 aagacggtgc gatgaacatc tacgacacca tccgccaaat cggtatttta aacagtcgcc
     4981 ccgacgcgcc ggtgcattgg cagttgagcg tgcagacgca caaatgggcg ggaatagagt
     5041 agtttaagca gtgtaactca aagggacggc gtacggtttt accgatgttt gacatatggg
     5101 aaaagtgtgc cgcttccgcg tgaaactgcc cacatttccg ctgcctgatt gaggcggagc
     5161 cttactgaat aagatgccgc cgttgggtac aagcccggct tcctaaattc cgatggtctt
     5221 ttgaaccttg ccgatactct gtgccagtgc gcgcaaatgg cagggttagg gaaaacgaaa
     5281 tgccgtctga aacaacattc tgagaccttt gcaaaaatag tctgttaacg aaatttgacg
     5341 cataaaaatg
//
LOCUS       DAWKOG010000142         5258 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.142, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000142 DAWKOG010000000
VERSION     DAWKOG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.142"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            112..807
                     /gene="pgl"
                     /locus_tag="WJK66_001730"
     CDS             112..807
                     /gene="pgl"
                     /locus_tag="WJK66_001730"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEZ7579400.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            788..1774
                     /locus_tag="WJK66_001731"
     CDS             788..1774
                     /locus_tag="WJK66_001731"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEZ7579401.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGASPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            1824..2672
                     /gene="hexR"
                     /locus_tag="WJK66_001732"
     CDS             1824..2672
                     /gene="hexR"
                     /locus_tag="WJK66_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HEZ7579402.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            2793..4439
                     /gene="pgi"
                     /locus_tag="WJK66_001733"
     CDS             2793..4439
                     /gene="pgi"
                     /locus_tag="WJK66_001733"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ7579403.1"
                     /translation="MKHLHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQHMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGPQKHDSSTERLINLYLQTNRK"
     gene            4453..5004
                     /locus_tag="WJK66_001734"
     CDS             4453..5004
                     /locus_tag="WJK66_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ7579404.1"
                     /translation="MPSERRPFQTAFLSNRKTVGKLAERVQSRYDGLHKFKHICSAAM
                     ALIKEPLDKAKQRNEELEAAEEAAAQEALGREQEVDRVSEWEERYKLSRSEFEQFWKG
                     LPQTVQNKLQASQKTWKSGMDKICANNAKAEGKTPNGIKFSELACKTAKTEARLEELH
                     NRKKALIDEMAREADKKELSKRL"
BASE COUNT         1337 a         1465 c         1391 g         1065 t
ORIGIN      
        1 aagcagtccc gatatttggt atcaaaagca taaacaactg ttcggccgac atattgctca
       61 acccgtttcc ataacggaat atgccgtctg aaataaaaaa aggacacaaa tatgtttgtt
      121 tggcacgaat acgaaaatgc ggcagaagcg gcgcagtctt tggctgacgc agtggcggat
      181 gctttgcagg gcgcactgga cgagaagggc ggtgcggtgt tggcagtttc cggcggacgt
      241 tcgccgattg catttttcaa cgccctgtcg caaaaagatt tggattggaa aaacgtcggc
      301 atcaccttgg cagatgaacg catcgtgccg accgtccacg ccgacagcaa taccggtttg
      361 gtgcgcgaat acctgttgaa gaacaaagcg gaagcggcaa tgtggattcc tatggtggaa
      421 gacggaaaaa ctgaaaccga attacatccc gatgctgttg tcgattatgc actgaaacat
      481 tacaaacagc ccgatgtttt ggttttgggt atgggaaacg acgggcatac ggcttcgatt
      541 ttcccgaaag ctccgcagtt tcagacggca atcgacggtt cggcaggtgt cgcgctggtg
      601 cataccacgc ccgttaccgc gccgcacgag cgcgtcagta tgaccttgga tgcgattgcc
      661 catacggggc atgtgttttt ggcgatacgg ggcgaagaga aaaaagccgt gttcgaccaa
      721 gccgcacaag gcgaaaaccg cgaatatccg atcaacctcg ttttgaacca tcaaggagtg
      781 aactgccatg tcttctacgc cgaataaaca agccggatat ccccgactgg tcgccgatat
      841 cggcgggacg aatgcacgct ttgcgctgga aaccgcgccg cgcgtcattg aaaaagccgc
      901 cgtgcttccg tgtaaagact acgatacggt taccgatgcg gtgcgtgcct atctgaatca
      961 aagcggtgca acagccgtac ggcacgcggc atttgccatc gccaacccga ttttgggcga
     1021 ctgggtgcag atgaccaacc accattgggc gttttccatc gaaaccaccc gtcagacttt
     1081 ggggctggac accctcatcc ttttgaacga ctttaccgcg caggcattgg cggtaacgca
     1141 gacttcaagc aaagacctga tgcaggtagg cgggcaaaag cctgtcgaat ttgcccccaa
     1201 agccgttatc ggccccggta ccggcctggg cgtgagcgga ttggtgcaca gccacgcagg
     1261 ctgggtggct ttggcgggcg agggcgggca taccagtttc ccgccgtttg acgatatgga
     1321 agtgctgatt tggcagtacg ccaaaaacaa atacggccat gtttccgccg aacgcttttt
     1381 gagcggcgcg ggcttgagct tggtttacga ggctttggct gcaaaacaga aagccaaacc
     1441 cgccaaactg atgccgtctg aaatcacgga aaaggctttg agcggcgcgt cgcctttgtg
     1501 ccgtcagact ttggacatct tctgcgccat gctcggcacg gttgcttcca acctcgccct
     1561 gacgctgggc gcgcgcggcg gcgtgtacct gtgcggcggc attattcccc gcgtgttgga
     1621 atatttcaaa acttccccgt tccgcagccg tttcgagaac aagggcaggt ttgaagcata
     1681 tcttgccgcg attcccgtgt atgtcgtctt gagcgagttt cccggaattt ccggtgcggc
     1741 tgcggctctc gacaaccatt tgagaaacgt ttaaccacag cggctccttg cagcggggct
     1801 gcattatcga agggcatatc attatgttaa gcaaaatcag cgaatcactg gcaaaccttt
     1861 ccggtgcgga acgcaaagtc gccgaatgtg cattggcgga acccaaatgg ttcgtccatg
     1921 ccgccgttgc cgaaattgcc gaacgtgcgt ccgtcagcca gccgaccgtc atccgattct
     1981 gccgcagctt gggttataaa gggctgcccg agttcaagct cgccttgtcc gccagcatcg
     2041 gtcatgaggg tatgccctat gtccacgaag aactcaacgc cgacgacgat atggcaagcg
     2101 tggtcgagaa agtgttgggc aatgccgccg cctcgctgtt gggcgaacgc cgcttcctga
     2161 aagagtcgga gctggaaaac gccattgcca cgctgatgca cgcccgtcgc gtcgagtttt
     2221 acggtgtcgg caattccggc attgtggcac aggacgcgca gcataaattt ttccgtttcg
     2281 gcatgtccac cgtcgcctat gtcgatacgc acacgcagct gatggcggca tctgttttga
     2341 gcgatcagga tgttttggtt gccatttcca acacgggttc gtctatcgaa cttttggatg
     2401 cggtcagcat cgccaaagaa aacggcgcgt ctgtcatcgc actgacccgc aacgattcgc
     2461 ctctggcgca acttgccgac tgcgtgttga gcgttgccac acaggaaaat gccgaactct
     2521 acacgcccat ggtttcccgc ctcttgcagc ttgccgtcat cgacattctc gccatcggac
     2581 ttgccctgcg cttgggcgat gctgccagcc tgcaattgca gaaaagcaaa aaaagcatac
     2641 acaacaagca catcgattac gacaaagatt gaccttcaga cggcatccca caaatgccgt
     2701 ctgaaatgcc gaacaacggt cgtcggcggc ttgcggcagt ttccggcagc cttttcatcc
     2761 cacaacaaaa aacctcattc aggagcatat agatgaaaca ccttcacgac ttacccgcat
     2821 ggtcgaaatt gtggaaccac tttgacgaca gcaaaacatt gcatatgcgc gaaatgttcg
     2881 agcaagaccc gcagcgtgcg gaacgctact ggctgcaagt cggcggactg acgctggact
     2941 actccaaaaa ccgcatcaac gacgaaacca tgtcgctttt gttcgagctt gcccgagaag
     3001 caggcgtgcc ggagcggatg cggcagatgt tccacggcga aaaaatcaat accaccgaaa
     3061 accgcgccgt cctgcatgtc gcccttcgca accgcaccaa ttcgccgatt gtggttgacg
     3121 gtgaagatgt gatgcccaaa gtcaaccgcg ttttgcaaca tatgggcgaa tttgcacacg
     3181 aagtccgcag cggaagctgg ctgggctata ccaaccaagt cattaccgac gttgtcaaca
     3241 tcggcatcgg cggatcggat ttgggtccgc tgatgatgtg taccgcgctc aaacctttcg
     3301 gtcatccgcg cctcaatatg cacttcgtct ccaacgtgga cggctcgcaa ctgcgcgacg
     3361 tattgtccaa agtccacccc gaaaccacgt tgttcatcat cgcctccaaa acatttacca
     3421 cgcaggaaac gctgaccaac gcgctgaccg cgcgcgaatg gtttttgaat catgcgggcg
     3481 acgaagaagc cgttgccaaa cacttcgccg ccgtttccac caatcaaaaa gccgtcgccg
     3541 aattcggcat cgacaccgcc aatatgtttg aattttggga ttgggtcggc ggtcggtaca
     3601 gcctgtggtc cgccatcgga ttgccgatta tgctgtatct cggcgaagaa aacttcatcg
     3661 aaatgctcaa cggcgcgcac ctgatggacc aacacttcat caacacaccg ctcgagcgca
     3721 acctgcccgt cattctcgcc ctcatcggca tctggtatat caactactac ggcggcggca
     3781 gccacgtcat cgcgccttac gaccaacatt tgcaccgcct gcccaaattc atccagcagc
     3841 tcgatatgga aagcaacggc aaacaggtta cgttggacgg caaagcagtc ggacacgaaa
     3901 cctcgccgat tatctggggc gaaacgggca ttaacggcca gcacgccttc ttccaactgc
     3961 tgcaccaagg cacgcacatt acccccatcg acctgattgc ctcgcttgaa aaacgcagca
     4021 acctgcccgg acaccacgaa atcctgcttg ccaatgtctt cgcccaagca gaagccttta
     4081 tgcgcggcaa aacccccgac gaagtccgcg ccgaactcaa agcgcagggt atggatgagg
     4141 cgcgcatcga agagctggtc ccgcacaaaa ccttctccgg caaccgcccg accaacctca
     4201 ttctcatgga caaggtcaac ccgcgcaata tgggcagcct gattgccatg tacgaacaca
     4261 aaaccttcgt acaaggcatc atttggggca tcaacagctt cgaccagtgg ggcgtggaac
     4321 tcggcaaaca actggctaaa accattttgg gcgaactgac cggcgaaacc gggccgcaaa
     4381 agcacgacag ttcgaccgaa cgcctgatca acctctacct gcagaccaac cgcaaataaa
     4441 acctgcggaa aaatgccgtc tgaacgccga ccgtttcaga cggcattttt atcgaacagg
     4501 aaaaccgtcg gtaaacttgc agagcgtgtg caatcccgat atgatggttt gcataaattt
     4561 aaacatatat gttccgcagc tatggcactg attaaagagc cgttggacaa agcgaaacaa
     4621 aggaacgaag aacttgaagc ggcagaagaa gcggcggcgc aggaggcatt gggtcgggag
     4681 caggaagtcg accgcgtatc cgaatgggaa gaacgctaca agctgtcgcg cagcgagttc
     4741 gagcagttct ggaaaggatt gcctcaaacc gtacagaata agctgcaagc ctcacagaaa
     4801 acatggaaaa gcgggatgga taaaatctgt gccaacaatg cgaaagctga aggtaaaacg
     4861 ccaaacggca taaaattcag cgaactggca tgcaaaacgg cgaaaaccga agcacgcttg
     4921 gaagagctgc acaaccgtaa aaaagccctt atcgacgaaa tggccaggga agcggacaag
     4981 aaagaactgt caaagcggct ctgaacagcg cggttcaggc actgcccgcc gatattgccg
     5041 aaaccgtcat gcccgagtac cgcaactgac aaaacggttt gaatgccaaa tgcgccgacg
     5101 gcgacgaata cggcgtggca caatctgact gccgtaccag agaaatcaat gcgaaaacca
     5161 aagaaatcca aggttatctg attgactgaa acttggatgc gggaatgtcg gcggcttttg
     5221 cgtttttgtc gtttttatag tgaattaaat ttaaaccg
//
LOCUS       DAWKOG010000143         5199 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.143, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000143 DAWKOG010000000
VERSION     DAWKOG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.143"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(45..461)
                     /gene="dksA"
                     /locus_tag="WJK66_001735"
     CDS             complement(45..461)
                     /gene="dksA"
                     /locus_tag="WJK66_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEZ7579405.1"
                     /translation="MAKLTEQDILNWSGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(608..1399)
                     /locus_tag="WJK66_001736"
     CDS             complement(608..1399)
                     /locus_tag="WJK66_001736"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEZ7579406.1"
                     /translation="MNVYFLGGGNMAAAVAGGLVKQGGYRIHIANRGAEKRERLQKEL
                     GVETSETLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLPVDTLSRYLGG
                     TRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDRRIADRIMKSVGLTVWLEDEAQMHSI
                     TGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGEDFEKLQ
                     KNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVRRSQEMERQYQ"
     gene            complement(1448..1856)
                     /locus_tag="WJK66_001737"
                     /pseudo
     CDS             complement(1448..1856)
                     /locus_tag="WJK66_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            complement(1856..2542)
                     /locus_tag="WJK66_001738"
     CDS             complement(1856..2542)
                     /locus_tag="WJK66_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEZ7579407.1"
                     /translation="MTVLQERYCEVSDRIGKLVLQAGRKPHSVSLIAVGKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSGQRPSSMPPLQVCIEVNIAGEAVKHGVAPEEAVALAVEVAKLPNIVVRG
                     LMCVAKANSSETELKVQFQTMRKLLADLNAAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2662..3705
                     /locus_tag="WJK66_001739"
     CDS             2662..3705
                     /locus_tag="WJK66_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEZ7579408.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNIGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHRSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3868..5094
                     /locus_tag="WJK66_001740"
     CDS             3868..5094
                     /locus_tag="WJK66_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ7579409.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
BASE COUNT         1291 a         1611 c         1175 g         1122 t
ORIGIN      
        1 ctgtctgaaa cttagggctt cagacggcat tttgtttgcc gccatcagtc ggcaaactgt
       61 tttttcatcc tctctcggcg ttcttgggac tcaacagata aagtggctgt cgggcgtgcc
      121 agcagccgct tcagaccgat aggctctccc gtatcggcac agaatccata atccccttca
      181 tcaatattgc ggatggtcgc ctgtatttta ctgagaagtt ttcgttcccg atcgcgggta
      241 cggagttcca atgcgtactc ttcttcctgt gtggcacggt cggcaggatc gggggctgat
      301 tcgtgttctt ggagatgccc tgtcgtagcg gaagcatttt cgatgagttc gtcttgcatt
      361 tttaccagca attcgcggaa aaaagccaaa tggtcgtcat tcatataatc gtcttccggc
      421 ccgctccaat tcaaaatatc ttgttctgtc agctttgcca taatgtatat cctctcggtt
      481 caccgaaaaa aacaaagcac cacaaagtgg ctttattcct ttgattttta taaggcttat
      541 tgcctaaggc gtttttgaaa acgcgcatca taacacgttt tggattggtt tttttatttt
      601 ctttacatta ttgatattgc cgttccattt cctgcgaacg gcgcacacag gcacaaacgc
      661 cctcacttat ggcttcggcg acacgatgcc gcctgaaggc ttccacggct tcgtgggttg
      721 tcccgccttt tgacgttaca tttttttgaa gcttctcgaa atcttcaccc gtctgctcgg
      781 caagggcaac cgctccttta aacgttgcca gactgagcgc gcgtgcttct gccatatcaa
      841 acccttgtcg gatggcggca ttttgcaatg cgtccagcag ataaaacaca tatgccggcc
      901 cgctgccgct gataccggta atgctgtgca tttgcgcttc atcttccaac caaacagtca
      961 aaccgactga tttcatgatt cgatcggcaa tcctgcggtc tgtttccgat acttccgctt
     1021 cggcatacat accggatacg cccagcccga tttttccggg tgtattcggc ataacccgga
     1081 caatgcggcg tgttcccccg aggtaacggc tgagcgtatc gaccggcaat ccggctgcga
     1141 cagaaagcac caatgcgccg ttggtgcgga tatttttgca cgcggcttcc atatcctgcg
     1201 gcttgacggc aaggattaaa acatcgtcgg aatgaagctc cggcaaggtt tcagaagttt
     1261 cgacccccaa ctctttttgc aaacgttcgc gcttttccgc accccggttg gcaatatgga
     1321 tgcggtaacc gccttgtttg accaatccgc ccgcaacggc agccgccata ttgccgccgc
     1381 cgagaaaata aacattcatt atttctaccc tattcaaaat tacctgacta aattcattcc
     1441 caatttttta ccatcggacg cgctcggaca gaccgttgcg ccgcaacccc tgctttttca
     1501 aacaatcaaa accttcctgt ttgaacgtct caatcaaagg tttgccgtca cgcgtacgcc
     1561 aatccaaccc gcaggcttca aacttcccta ttttcctgta aaagtaagat tgttgtgcgg
     1621 attcatattc ctttgccctg acggaacgat tgccgtctga aagcggtatc tcgtagtcct
     1681 ctaaagacgg cggactctcg ccgccaatat cccaaaaatc gatggcagcc ggttttctcg
     1741 gcttacacca gccggacagg gacgaacact caataccaac ccgccaaccg gcagcctgca
     1801 accgtaaatg caagcaacgc agccattgcc gtatatttta ttgcccgaat ttccatcagc
     1861 cccttttccc gaaaatcgcg ctgccgatac ggacgtgtgt cgcaccgcac tcaatggcgg
     1921 caggcatatc gtccgacatc cccatagaca gcacgtctgc cttaacgcca gccgcattga
     1981 ggtcggcaag cagtttccgc atcgtttgaa attgcacctt caactccgtt tcactgctgt
     2041 tggctttggc aacacacatc agtccgcgta cgacgatatt cggcagcttc gccacttcca
     2101 cagcaagcgc gactgcttct tcgggcgcga caccgtgctt caccgcctcg cccgcaatgt
     2161 tcacctcaat acacacctgc aaaggcggca ttgaggaagg acgttgcccg ctcagccgga
     2221 cagcggtctt tagacggcat acggtatgca cccaatgcgc gcgttcggcg acaaacttgg
     2281 ttttgttgga ctgcacatcg ccgatgacgt gccacacgat gtcggtcaaa tccgccaact
     2341 cttccgtttt gccgtaccac tcctgaatat agttctcgcc gaaatcacgc tgtccggcgg
     2401 cgtaaacttc gcggatgccg tctgaaggga aagtcttacc gacggcaatc aggctgacgg
     2461 aatgcggctt ccttcccgcc tgcagaacca attttccgat acggtcggac acctcacaat
     2521 aacgttcttg caacaccgtc atagattatc ccctaattaa aaatgattaa acagttgaaa
     2581 cccctccagt cagggcggta caatcaaggt tgttagaacc attccaacca atcgaaacat
     2641 tatactaaac agagccgcat tatgcagatt accgacttac tcgccttcgg cgctaaaaac
     2701 aaagcatccg accttcacct gagttcgggc atatccccta tgattcgggt tcacggcgat
     2761 atgcggcgca tcaaccttcc cgaaatgagc gcggaagagg tcggtaatat ggtaacttcg
     2821 gtgatgaacg accaccagcg gaaaatctac cagcaaaact tggaagttga cttctcgttc
     2881 gaactgccca acgtcgcccg attccgcgtc aacgccttca acatcggccg cggtcccgcc
     2941 gccgtattcc gcaccattcc cagcaccgtc ttatcgctgg aagaattgaa agccccgagc
     3001 attttccaaa aaatcgcaga atcgccgcgc ggcatggtat tggttaccgg ccctaccggt
     3061 tcgggcaaat cgaccacgct tgccgcaatg atcaactaca tcaacgaaac ccagccggca
     3121 cacatcctga ccatcgaaga cccgatcgaa ttcgtccatc gaagcaaaaa atccctgatt
     3181 aaccagcgcg agctgcacca gcacaccctc agcttcgcca acgcgctgcg ttccgcattg
     3241 cgcgaagacc ccgacgttat ccttgtcggc gagatgcgcg acccagaaac cattggcttg
     3301 gcactgaccg ccgccgaaac cggacacttg gttttcggca cgctgcacac gaccggcgca
     3361 gcaaaaaccg tcgaccgtat tgtggacgta ttcccggcgg gagaaaaaga aatggtgcgc
     3421 tctatgctgt ccgaatcgct gaccgccgtc atctcccaaa acctgctgaa aacgcacgac
     3481 ggcaacggcc gtgtcgcctc gcacgaaatc ctgattgcca accccgccgt ccgcaacctc
     3541 atccgcgaaa acaaaatcac gcagattaac tccgtcctgc aaaccgggca ggcgagcggt
     3601 atgcagacaa tggaccaatc gctgcaatcg ctggtgcgcc aagggctgat cgcaccggaa
     3661 gtcgcacgca gacgcgcgca aaacagcgaa agtatgagtt tctgacatac aaccgccttc
     3721 cggccatacc ggcgggaaaa caaggcgcaa acacgcgggg cgggacgcag catcccgccc
     3781 ggctaccttt ccgaacaaga agcgtccgcc ttcctgttga aacctgccgc cgcaaactgc
     3841 aaggcttaaa ccgaaaagaa gctaacgatg aataccgata acctgcacga catcttggac
     3901 gaaatggttc aagtgtattc tcaaaaaaaa caaagccgat ccgaaacccc ggccgaaatc
     3961 ggcgcacact tccacccgct gctcgaccgc ctgtgcgaaa ccgcagaagc acaaaacgcg
     4021 tccgacatcc ttatcagcaa aggattcccg ccctcgttga aaatcaacag cgcattaacc
     4081 ccgcagccgc aaaaggcgct gacgggcgag gaaaccgccg ccatcgccgc atcgacgatg
     4141 aacgccgaac aatcggaaat attccggcgc gacggcgaaa tcaactactc cgtccagtcg
     4201 cgcagcggca cgcgctaccg cgccaacgcc taccacagcc aaggcagcgc aggtttggtt
     4261 ttgcggcgca tcaaccacgt catcccgcaa atgcaggaat tgggcctgcc cgaaaaactc
     4321 aaagacctcg ccgtcgcacc gcgcgggctg ctgattatcg tcgggcctac cggttcgggc
     4381 aaatccacca cgatggcgac tatgctcgaa caccgcaaca aaaccctgcc cagccatatc
     4441 gttaccatcg aagacccgat tgagtttatc tacaaaccgc gccgctgcat ctttacccag
     4501 cgcgaaatcg gcgtcgacac cataaactgg cagacggcgg tacaaaacgc tatgcgccaa
     4561 tcccccgacg tggtctgcat cggcgaagtc cgcagcaggg aaagtatgga atacgcgatg
     4621 cagctcgccc aaaccggcca cctgtgcatt tttacgctcc acgccaacac cgcgccgcag
     4681 tcgctcgaac gcatactcaa cttctacccc aaagaacagc acaaccaaat actgatcgac
     4741 atcgccctca acctgaccgg catcatctgc caacgcctcg ccctcaaaca agacaaaacg
     4801 ggcaggacgg cggttgtcga cttgctcatc aacacgcccg ccatccaaga cttcatcctg
     4861 aagggcgacc tgatgaacat cagtaaaatc atggaaaccg ccaaaaccga cggaatgcag
     4921 acgatggatc aaaacctttt cgaactgtac cgtcacggca tcatcagtta cgaagaagcc
     4981 ctgcgccagt ccgtttccgc caacaacctg cgattgcaca tccaactgca caaagaaggc
     5041 aaaacgcccg aactccttta cgacagggtc aacggtctca acctcatttc ctgatccgca
     5101 aaacccaatg ccgtctgaaa accgcatccc cgttttcaga cggcatgatt ttatccgccc
     5161 cattcatgtg ctacacttta tagtggatta aatttatag
//
LOCUS       DAWKOG010000144         5123 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.144, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000144 DAWKOG010000000
VERSION     DAWKOG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.144"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(148..1191)
                     /locus_tag="WJK66_001741"
     CDS             complement(148..1191)
                     /locus_tag="WJK66_001741"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEZ7579410.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPDLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEVGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGICPETILRDYYRDSLFVDI
                     LPAGSTPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     KETHGLGAIPLLP"
     gene            complement(1188..1481)
                     /locus_tag="WJK66_001742"
     CDS             complement(1188..1481)
                     /locus_tag="WJK66_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579411.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPADFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQAVGQRSDGIPANHTKEKP"
     gene            1849..2169
                     /locus_tag="WJK66_001743"
     CDS             1849..2169
                     /locus_tag="WJK66_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEZ7579412.1"
                     /translation="MSIYLDFGISPKTFRQTYLYQKPKLFKGAVRNLEAASWKDINEI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGASLLYNH
                     IIVD"
     gene            2188..2997
                     /locus_tag="WJK66_001744"
     CDS             2188..2997
                     /locus_tag="WJK66_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ7579413.1"
                     /translation="MPRLAGAHTIVSGYLAFGSDESYKNHWDIRDVYAIQLFGKKRWQ
                     LTAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHL
                     AVGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYE
                     AFSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKIS
                     VDELGKKVLEHIGKNEPLLLKNLLVNFNQGKHEEVRKLIYQLIELDFLELL"
     gene            3226..3579
                     /locus_tag="WJK66_001745"
     CDS             3226..3579
                     /locus_tag="WJK66_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579414.1"
                     /translation="MIELQLHELKLVSGGGPVTDNIAGNVANAATTKGGPTWGDFVAI
                     PAAAATVHFLPKNFFGAIGANGVYNLTRDWVNDAVNAPPYNGRPIFEIEHGLTAPATK
                     ADKSGNGYTDGTDYC"
     gene            3640..4356
                     /locus_tag="WJK66_001746"
     CDS             3640..4356
                     /locus_tag="WJK66_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HEZ7579415.1"
                     /translation="MNRPKQPFFRPEVAVARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTVEGQILPASGVIRVYAPDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGDSVQQQLKTEAVLKKTLAEQELGRLKLIHGNETRSLKATVERLENQELH
                     ISQQIDGQKRRIRLAEEMLQKYRFLSANDAVPKQEMMNVKAELLEQKAKLDAYRREEV
                     GLLQEIRTQNLTLASLPQAA"
     gene            4421..4915
                     /locus_tag="WJK66_001747"
     CDS             4421..4915
                     /locus_tag="WJK66_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEZ7579416.1"
                     /translation="MRSEQIIRAGRSGYIAIPNVEVGQQVDPSKLLLSIVPERTELYA
                     HLYIPSSAAGFIKPKDKVVLRYQAYPYQKFGLASGSVVSVAKTALGRQELSGLGMVSS
                     DLAKSNEPVYLVKIKPDKPTITAYGEEKPLQIGMTLEADILHEKRRLYEWVLELIYSM
                     SGKL"
BASE COUNT         1387 a         1165 c         1282 g         1289 t
ORIGIN      
        1 attcccgctt ttgcgggaat gacggtattt tagatttttg ttttcgcgga ataatgagcc
       61 aaaagccgtc cgaacccaaa aaaaccgtcg ctttcagacg gcatcaaaaa accgcaggca
      121 cgatgcctgc ggtttgccgt ttgcgcttca ggggagcagg gggattgcgc ccaagccgtg
      181 tgtttccttc agtccgaaca tgatgttcat attttgcacc gcctgacccg ccgcgccttt
      241 gacgaggttg tcgatgacgg aaaggacgac ccacacatcg gattgcgccg cctgttggat
      301 gctgatgcgg cagaggtttg cgccgcgcac gctgcgggtt tcgggtgtcg aaccggcggg
      361 caggatgtcc acgaacaggc tgtcgcggta atagtcgcgc aggatggttt cggggcagat
      421 gccgtctgaa aggtggaggt aaacggtggc gtgcataccg cgtatcatcg gcgcgaggtg
      481 cggcgtgaac acgaatcctt cggcgatgcc gtcctgaagc ccggcgatgg tctgcctgat
      541 ttcgggcagg tggcggtgtc cagctatgcc gtatgctttg aagttgtcgc cgacttcgca
      601 caacagcgaa ccgacattgc ctttcctgcc cgcgccggac acgccggatt tgcagtcggc
      661 aatcagcggc ataccgggct tcagacggca ttgccgcaac agcggcacga gcggcaggga
      721 tacgcaggtc gggtagcagc cggggttggc gacgaggcgt gcctgtgcga cggcttcgcg
      781 gttgagttcg ctcaatccgt acacggcttg ggaaacgagg tcgggggcgg cgtgggtcat
      841 gccgtaccag tgttcccagg tcggaatgtc ccgtatgcgg aagtcggcgg aaaggtcgat
      901 gacgcgcacg ccctgttcaa tcaggcgcgg cgcgtctttc atggcgatgc cgttgggcgt
      961 ggcgaagaag acgatgtcgc attgttccaa acctgcctcg tcgggcgttt ggaaggcgag
     1021 gccgtacacg ccgcgcaaac tcggaaagta atcggcaact gcagttcccg cttcgctgcg
     1081 gctggttacg gcggcgactt cgacatcggg atgggcggca agcaggcgca gcagttccac
     1141 gcccgtgtag cccgtcgcgc cgacaatgcc gactttgatt tttttactca tggtttttcc
     1201 tttgtgtggt tggcgggtat gccgtccgaa cgctgtccga cggcttgtcc gacggggttt
     1261 tgattggcgg cggaaggacg gtacacaaag cgtttgaaaa gcgtgtctaa tttaatggca
     1321 tccgcctctg tttttaaaac ccagccctgc cgtccggaat aaacataacc gtaggcgcga
     1381 aagtcggcag gatatttcgg tgcctgcccc aaacccaaac cgtatgcggc gaaagcggtg
     1441 gtcgtggaga aggaaagcag gaggaggacg agggttttca taaggttcca aaccgaacgg
     1501 ggcgatgaag cccgattata gcaaaaggtc gtctgaaaac cgttttcaga cgacctttta
     1561 tttaattaca ataaaaatgt taacaacaaa aaacaaaccg ctttttttcg tttggaaaca
     1621 aaacaaattt ttatagtgga ttaaatttaa accagtacag cgttgcctcg ccttgccttg
     1681 ccgtactatc tgtactgtct gcggtttcgt cgccttttcc tgatttttgt taatccacta
     1741 taactaaaat tgtcaacaat tccttgacgc acacacaaaa aaaactttaa tatttgtaaa
     1801 tatatgtaac attataaatt ttaaatattt tctgagtaag gggaagtaat gagcatctat
     1861 ctcgacttcg gcattagtcc taaaacgttc cgacagactt atctgtatca aaagcccaag
     1921 ctctttaaag gagcggttcg gaatctcgaa gccgcatctt ggaaagatat caacgagata
     1981 taccaacgag cagacccaac cgcaccgctg tttcatctgc gtaaaaaagg tgcaattgtt
     2041 cctaaagaag aatacgtcga aagtttcgac gatttgggca aaactcgcta ccgttttatt
     2101 aaatccgtta tctacgaaca tatgaagaat ggtgcgtcgt tgctctataa ccatattata
     2161 gtggattaaa tttaaaccag tacagcgttg cctcgccttg ccggcgcaca tactattgtt
     2221 agtgggtatc ttgcttttgg cagcgacgaa tcttataaaa accattggga catccgagat
     2281 gtgtatgcca tccagctttt cggcaagaaa cgttggcaac ttactgcccc tgatttccct
     2341 atgccattgt atatgcaaca gactaaagat actgatattt ccattcctga acatatcgat
     2401 atggatatta tccttgaagc aggtgatgtc ctctacatcc cgcgcggttg gtggcacaga
     2461 cctatcccgc tcggctgtga aaccttccac ctcgctgtcg gtacctttcc gcccaacggc
     2521 tataattacc tcgagtggct aatgaagaaa ttccccacca tagaaagtct gcgccacagt
     2581 ttctcagact gggagcaaga taggacgcgt atcaacgata ctgccgcaca aattgctgcc
     2641 atgattgccg accccgtcaa ttacgaagcc ttcagtgaag acttcctcgg caaagaacgt
     2701 accgataccg cttttcatct cgaacagttc gcgaatccca acgctactcc gctttcagac
     2761 gacgtcaggt tgagactaaa tgccaataat ttggatacgt tggaaaaggg atatttgatt
     2821 gggaatggga tgaagataag cgtagatgag ttggggaaaa aagtgttaga acatatcggt
     2881 aagaatgaac cgttattgtt gaaaaatcta ctggttaact tcaatcaggg aaaacatgaa
     2941 gaagttagga agttgattta tcagttgata gagttagatt ttctggaact tttgtgaggg
     3001 attctatgaa aaactggaag caatttacat ttttcgtaat attagtaata gcttgttatc
     3061 aagtgctata ttttttatca gatatgtttc ttctcgacta cattaacaaa tatagttgga
     3121 atttgaattt tattcagggt actttgaatt ttttttcaat atatctgcca tatgtttttg
     3181 ttagccgaat ttttaggaat accaaccaag aaaaggagta taaaaatgat tgaacttcaa
     3241 cttcatgaat tgaagctggt ttcaggggga ggtcctgtaa cagacaatat agctggaaat
     3301 gtagctaatg ctgccacaac caaaggaggt cccacatggg gggattttgt tgcaatacct
     3361 gctgctgcag cgacagttca tttcctgccg aaaaattttt tcggtgctat tggtgcaaat
     3421 ggtgtataca atctgactcg tgattgggta aatgatgccg ttaatgcacc tccttataac
     3481 ggaagaccaa tctttgagat tgaacatgga ttaactgctc ccgcaacaaa agcagataaa
     3541 tcaggaaacg gctacactga cggtacagat tactgctgat atttctcatc tttcagacaa
     3601 tctctagggg tcgtctgaaa ctttcttaac ttcaatttta tgaatagacc caagcaaccc
     3661 ttcttccgtc ccgaagtcgc cgttgcccgc caaaccagcc tgacgggtaa agtgattctg
     3721 acacgaccgt tgtcattttc cctatggacg acatttgcat cgatatctgc gttattgatt
     3781 atcctgtttt tgatatttgg taactatacg cgaaagacaa cagtggaggg acaaatttta
     3841 cctgcatcgg gcgtaatcag ggtgtatgca ccggatacgg ggacaattac agcgaaattc
     3901 gtggaagatg gagaaaaggt taaggctggc gacaagctat ttgcgctttc gacctcacgt
     3961 ttcggcgcag gagatagcgt gcagcagcag ttgaaaacgg aggcagtttt gaagaaaacg
     4021 ttggcagaac aggaactggg tcgtctgaag ctgatacacg ggaatgaaac gcgcagcctt
     4081 aaagcaactg tcgaacgttt ggaaaaccag gaactccata tttcgcaaca gatagacggt
     4141 cagaaaaggc gcattagact tgcggaagaa atgttgcaga aatatcgttt cctatccgcc
     4201 aatgatgcag tgccaaaaca agaaatgatg aatgtcaagg cagagctttt agagcagaaa
     4261 gccaaacttg atgcctaccg ccgagaagaa gtcgggctgc ttcaggaaat ccgcacgcag
     4321 aatctgacat tggccagcct cccccaagcg gcatgagaca gaacaaagct ggcttgaacg
     4381 caccattgcc gatatttctc aagaagtttt ggattttgaa atgcgctctg aacaaatcat
     4441 ccgtgcagga cggtcgggtt atatagcaat accgaacgtc gaagtcggac agcaggttga
     4501 tccttccaaa ctgctcttga gcattgttcc cgaacgtacc gagctatatg cccatctata
     4561 tatccccagc agtgcagcag gctttatcaa gccgaaagac aaggttgtcc tacgttatca
     4621 ggcatatccc tatcaaaaat tcgggcttgc ttccggcagt gtcgtatcag tagcaaaaac
     4681 ggcactgggc agacaggaat tgtcgggatt gggcatggta tcctccgatt tggcgaagag
     4741 caacgaacct gtttatctcg tgaaaataaa acccgacaaa ccaaccatca ctgcatacgg
     4801 tgaggaaaaa ccgctgcaaa tcggcatgac gttggaagca gacatcctgc acgagaaacg
     4861 gcggctgtac gaatgggtat tggagctgat ttatagtatg tcgggcaaac tgtaagactt
     4921 gaggctgaaa gataaagaag ttgtctgaaa ccttttcaga cgaccagaag acaatatata
     4981 aaaacacaaa cttcaaaact gcctgaaacc atcccctttt acaggcactc cgtctacagg
     5041 cactccgttt gtaaaagcaa ggatagggca tctgcccttt tgattgtttg attaacgata
     5101 caaggagttt caaaatgaga gtt
//
LOCUS       DAWKOG010000145         5108 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.145, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000145 DAWKOG010000000
VERSION     DAWKOG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.145"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(90..1445)
                     /locus_tag="WJK66_001748"
     CDS             complement(90..1445)
                     /locus_tag="WJK66_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEZ7579417.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISSDIDVLNQ
                     NIYNKITTVGKDLVATAKHLSAKYGVPIVNQRISVTPIAQIAAATKADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINIGSTRAGINM
                     DAVRLAGETIKRTAEITLEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(1460..1732)
                     /locus_tag="WJK66_001749"
     CDS             complement(1460..1732)
                     /locus_tag="WJK66_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEZ7579418.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCPKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(1857..2594)
                     /locus_tag="WJK66_001750"
     CDS             complement(1857..2594)
                     /locus_tag="WJK66_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEZ7579419.1"
                     /translation="MKKLSRIVFSTVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAEGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGNIKTEMVMVNDEPAKILTWDESGRLLSELSIHHHKRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(2875..3774)
                     /gene="prmB"
                     /locus_tag="WJK66_001751"
     CDS             complement(2875..3774)
                     /gene="prmB"
                     /locus_tag="WJK66_001751"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ7579420.1"
                     /translation="MFNQAAQELTTIRDILRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGNGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            3919..4512
                     /locus_tag="WJK66_001752"
     CDS             3919..4512
                     /locus_tag="WJK66_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579421.1"
                     /translation="MADGMGNLIGWEKTGLVVGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGALTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     ENSGHELKVTWQGVKHAASKANQAELSIMTKLDDLLRYAKYEGSYSDRKGHPNIIAAH
                     KYRAVAKVGNKSLNIGVIVREFPDDHKHYDHFILKDE"
     gene            4624..>5108
                     /locus_tag="WJK66_001753"
     CDS             4624..>5108
                     /locus_tag="WJK66_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEZ7579422.1"
                     /translation="MTKLYAEIAKMETQDDDTVKVWGYASSEEIDSDGEVIAATAMKA
                     AIPDYMKFGAGREMHGSNAAGTAIEINVEDDGITFFGAHIIDPVVVSKVKTGVYKGFS
                     IGGSVTARDDLNKSQITGLKLTEISLVERPANPDAVSTCFKADKGAETVNNDTEHNAT
                     Y"
BASE COUNT         1410 a         1300 c         1130 g         1268 t
ORIGIN      
        1 attcttttct aaagacattt tacccgatgc ctataaacaa aaatgccgtc tgaagccttt
       61 cagacggcat ttccccatca aaaccgcaat cagtttttca tcgattgaac cggtgcggga
      121 atcctgccgc cccggttgac gaacacttcg catgagcctt cttttaccgg cattacaggc
      181 gcgtagccca acaggccgcc gaactcgacg ctgtcgccga cggttttacc ggttaccgga
      241 ataatgcgca cggcagtggt tttgctgttg atcatgccga tggcggcttc gtcggcaatg
      301 atgccggaaa tggtgtgcgc gggtgtgtcg ccgggaacgg caatcatatc caagccgacc
      361 gaacaaacgg cggtcatcgc ttcgagttta tccaacgtca gcacgcctgc ttcggcggcg
      421 gcaatcatac cttcgtcttc ggaaacgggg ataaacgcgc cactcaaacc gccaaccgcg
      481 ctcgaagcca tcatgccgcc ctttttcacg gcatcgttca gcaatgccaa agctgctgtt
      541 gtgccgtgcg taccgcagac gctcaaaccc atttcttcaa gaatgcgcgc caccgagtcg
      601 ccgacggcag gggtcggtgc cagcgacaag tcgagaatac caaacgggat attcagcatt
      661 tttgaggctt cgcggccgat gagttcgccc acgcgggtaa ttttgaaggc ggttttcttc
      721 acaacttcgg caacttcggt caatgtcgtt gcatccgaat tttccaacgc ggcttttacg
      781 acacccgggc cggatacgcc gacattaatc acagcatccg cttcgcctga gccgtgaaac
      841 gcgcccgcca taaacgggtt gtcttccacc gcgttgcaga acacgacgat tttggcgcag
      901 ccgaaacctt ctagtgtgat ttcagccgtg cgtttgatgg tttcgcccgc cagtctgacc
      961 gcgtccatat tgataccggc gcgcgtactg ccgatattga tggagctgca cacaatatcg
     1021 gtggtcttca tcgcttcggg aatggagcgg attaacacct catccgaagg cgacatgcct
     1081 ttttgcacca acgcggaaaa gccgccgata aaggacacgc cgatggcttt ggctgccttg
     1141 tccaaagtct gcgccacgct gacataggaa tcggctttag ttgccgccgc gatttgggca
     1201 atcggcgtga cagaaatgcg ctgattcaca atcggcacgc cgtatttggc ggaaaggtgt
     1261 ttcgccgttg ccaccaagtc tttgccgact gtggtgattt tgttgtaaat attttggttt
     1321 aacacgtcga tgtcgctgct gatgcagtcg tgcaaatcga tgcctatggt aatggtgcgg
     1381 acatcaaaat tctggtcggc aaccattttg acggtttcta aaatttcgcc ggattggata
     1441 ctcatcacat tcctccgact caaatgcggt gcatcgcttg gaagatttct tcattttgca
     1501 tacggatatc aagcgcgagt tttttgctct cttccgcaaa caaatccaaa acctcctgcc
     1561 gagatttggg gcatttcgag gtatccacca agataatcat ggtaaaaaaa tcatccatca
     1621 gctgttggct gatattaagg atgttgattc ggttttccgc caaaattttg gaaacatcgt
     1681 acacgatgcc gacgcggtct ttaccgatga cggtgatgac tgaattgttc acaggcttac
     1741 tccttgcaga tatccgttaa agtccgaaat tataccacca tcggattttg aggaaatatt
     1801 gtcaacaata tatacataca aaatgccgtc tgaaactatt tcagacggca tcaaatttag
     1861 ggttcgatta aataaccatc cttatcccac tgggttttcc taaccaactt atcatcctga
     1921 taaacagctt cactcttttt agaaccatct tcataccact ctaagactac tccattacgt
     1981 ttatgatgat ggatagacag ttccgagagt aatcgaccgc tttcatccca tgtcagaatc
     2041 ttggcaggct catcattgac cataaccatt tccgttttga tattgccgtc ggcataccat
     2101 tgcttccata cgccgtttgc cttattctgt ttaaactgga tttcgctttc cttgccgccg
     2161 ttacggtaat agcggtaccc cgtaccttca ctcaaaccat ttttataagg cataacggca
     2221 gattttttac cgttcggata ccagttgacc cactccccgt ccggcttacc cttgctgaag
     2281 ccccccgcca tttttttctg accattaaaa tgccacaaaa tcaacatacc gttttgcagg
     2341 gtaggcacaa aagatttgat ttgcgttgaa gcaacgatat aaggttcgga atatttcttc
     2401 atcgacggat aataaaaatc ctgcgcgtgc gcaataccct ccgccacact atattgcctg
     2461 atataagcgg cagaagacat cgtcgccgtc agtttcccgt tctgattaaa ataaacagaa
     2521 taggtctgcg ccggcaaagc ggccgaaaaa cccaacagga cagttgaaaa tacaatccga
     2581 gataattttt tcattgcaat agcgatataa aaacaaggct gtgttttagt aatcttttga
     2641 tttcaattat ttgcaaggga aaagacaatt attttccggt taggaataaa cctattctat
     2701 tgaatatatt gaagccaagt acgcctatca acactatatt aaaacactgc caaaaacaat
     2761 taacttataa acaatatggt aaggatttct ctgccaagca tcaaacccga gacaacgtat
     2821 cgtaaaaatg ccgtctgaaa acaaatcgtc ttcagacggc atttcccctt caactcactc
     2881 ttcacccaat aactgctcgc gcgtcaagag gaaaacaaaa ccgtcgcccc cgctggtttc
     2941 caaccaagta aaaggcaact ccggatacgc tgcttccaat acatccctgt tatgcccgat
     3001 ttccaccagc aatacacctt tgggattcag aaactttgcc gcattcagaa gaatctgcct
     3061 ggtggcatcc aacccgtccg ccccgctgcc caatgccaat tccggttcgt gcaaatactc
     3121 ttcaggcaat aactcaaccg attccgcatc cacataaggc ggattggaaa caatcaaatc
     3181 ataagtgcct tccaatcctt caaacaaatc cgtatgaata agccggatgc gttcttccaa
     3241 accataatct tcgacattaa tccctgccac ttccaaagca tccaagctca catcaaccgc
     3301 atcaatttgg gcatcaggat aatgatgcgc catctgaatg gcaaggcaac cgcttccggt
     3361 gcaaagatcc aaagcattat gcaccagctc atcgtattct atccaaggac gaagtccgtt
     3421 acccaacaat tcataaataa aagaacgagg tatgattacg cgctcatcca catagaaatc
     3481 aaactctccc tgccatgcct ggtgtgtcaa ataagcggct ggaatgtgtt cgacagcacg
     3541 acgctcaata accgccagca cttcctcttt ttcagcttcc aagagttttg catcaagata
     3601 tggggcaagc atatccaaag gcaaattcaa agtatgcaga atcaaataag ccgcttcatc
     3661 atgcgcatta tctgttccat gaccaaaaaa gagccctgcc tcattaaaac ggctgactgc
     3721 aaaacgtaaa atatcgcgga tagtcgtcaa ttcttgtgct gcctgattaa acataatatg
     3781 aaccattctg cgtatagata cttttaatta taacagaaac aacaagcaaa ccttttcata
     3841 tcgccaaata accacccaat ctacccatac aactacataa atgcccgcgc gaaaaccatc
     3901 gcccgaacgg aaacgacaat ggccgacggt atgggcaatc tgattggctg ggaaaaaacg
     3961 gggcttgttg tcggtaagca gtggataacc gcaaaagacg acaaggtgtc cgatgtctgc
     4021 aatgccaacg gcgagatggg cgtaatcggg ctttacgagc ctttctcaca cggcgcattg
     4081 acgatacccg gtcatccgaa ctgccgatgc gaggttgttt ccgtatcggg tggcgaattg
     4141 ggggaatttg ccgaaaaaaa ggagcttcgt aaagcggcta tgcagtatgc gcgggataac
     4201 tttatcggca aaagctatgt caatgaaaac agcgggcatg aactgaaggt aacttggcaa
     4261 ggtgtgaaac acgctgcgtc aaaggcaaat caggcggaat tatccatcat gacaaaactt
     4321 gatgacttat tgcgctacgc aaaatatgag ggttcttatt cggataggaa aggtcatcct
     4381 aatattattg cagcacataa gtatcgtgcc gttgccaagg ttgggaataa gtctttaaat
     4441 atcggtgtga ttgtaaggga atttccagac gaccataaac attacgacca tttcatcttg
     4501 aaggatgaat aaagcccttt tgcagtgtcg ttctggagcg gatagcgtta aggcaagtac
     4561 acttccagcc ttgaaaaagg gctttaaatt cagcatgcca tttatacagg caggagtaaa
     4621 cccatgacaa agttatacgc agaaatcgcc aagatggaga cgcaggacga cgacacggtc
     4681 aaggtttggg gttacgcttc aagcgaggaa atcgattcgg acggcgaagt catcgcggcg
     4741 acagctatga aggcggcgat tcccgattat atgaagtttg gcgcggggcg cgagatgcac
     4801 ggctcaaacg ctgcgggaac ggcaattgaa atcaacgttg aagacgacgg cataaccttt
     4861 ttcggtgctc atatcatcga ccccgttgtc gtgtcgaaag tcaaaacagg cgtttacaaa
     4921 ggcttttcca tcggcggcag cgttaccgcc cgcgatgatt tgaacaagtc gcaaatcacg
     4981 ggcttgaagc tgacggaaat cagccttgtc gagcgccctg ccaatcccga tgcggtgtct
     5041 acctgcttta aggcggacaa aggtgcggaa acggtaaaca acgatacaga acataatgct
     5101 acatattt
//
LOCUS       DAWKOG010000146         5048 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.146, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000146 DAWKOG010000000
VERSION     DAWKOG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.146"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            242..1474
                     /gene="pilG"
                     /locus_tag="WJK66_001754"
     CDS             242..1474
                     /gene="pilG"
                     /locus_tag="WJK66_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HEZ7579423.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            1548..2408
                     /locus_tag="WJK66_001755"
     CDS             1548..2408
                     /locus_tag="WJK66_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEZ7579424.1"
                     /translation="MSDLSVLSPFAVPLTAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            2410..3042
                     /locus_tag="WJK66_001756"
     CDS             2410..3042
                     /locus_tag="WJK66_001756"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEZ7579425.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEVFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAVYGIVEIPLLTEKRQFISLIRRVLTISAPVEKRIGRVMARSGLTRGEVAAVISH
                     QASESERLLLADDVLLNDGSLKSLREKTMRLHAFYSGIFASKPTQGKHND"
     gene            3035..3244
                     /locus_tag="WJK66_001757"
     CDS             3035..3244
                     /locus_tag="WJK66_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEZ7579426.1"
                     /translation="MTESRQTRLQVKCPTCQTAVVWKPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSSQTESLPEISEPDMAYR"
     gene            complement(3329..5005)
                     /gene="pilB"
                     /locus_tag="WJK66_001758"
     CDS             complement(3329..5005)
                     /gene="pilB"
                     /locus_tag="WJK66_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEZ7579427.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
BASE COUNT         1205 a         1402 c         1257 g         1184 t
ORIGIN      
        1 tttttgttaa ttcactatac cctccggccg gcatttgacc cgcaacaata tcctgcaaga
       61 tttattgtgt acgcataaat gccggacagc cgcctaaata aggtataatc gcatccgatt
      121 ctgtcccgtt tgccgcccgc aggcgggatg tcggcggttt tcatcttacc gggaacacgc
      181 cggtactgtt ttttaacgat gtttcttaca ttttattcca attactttac ggggctagaa
      241 tatggctaaa aacggaggat tttctttgtt cgcaaagaaa gaaaaacgct ttatctttga
      301 aggcaggcat tccgcctccg acaaactggt caacggcgaa gtatccgcgt ttaccgaaga
      361 agaggcgcgc aaaaaactgg caaaacgcgg catccgcccg ttgcagatta cccgtgtgaa
      421 aacaagctcc aagcgcaaaa tcacacaaga agacatcacc gttttcaccc gccagctgtc
      481 cacgatgatt aaagcgggcc tgccgctgat gcaggcattt gaaatcgtgg cgcgcggaca
      541 cggcaacccg tctatgacgg aaatgctgat ggaaatccga ggcgaagtgg aacagggcag
      601 ctcgttgagc cgcgcattct caaaccaccc aaaatatttc gaccgcttct actgcaatct
      661 ggttgcggcg ggcgaaacgg gcggcgtatt ggaaagcctg ctggacaaat tggcaattta
      721 caaagaaaaa acccaggcca tccgcaaaaa ggtaaaaacc gcactgacct atccggtatc
      781 cgtcatcgcc gtcgccatcg gtttggtatt cgtgatgatg attttcgtac tgcccgcctt
      841 taaagaagtt tacgccaata tgggcgcgga gcttcccgca ctgacccaaa cagtgatgga
      901 tatgtccgac tttttcgtct catacggctg gatggtgctg atcgcactgg gctttgccat
      961 atacggcttc cttaaattga aggcgcgttc gattaaaatc caacggcgta tggatgccat
     1021 actgctgcgt atgccgattt tcggagacat tgtccgcaaa gggacgattg cccgctgggg
     1081 caggacgacg gcgacgctga ttgcggcagg cgtgcctttg gtcgatgtat tggactccac
     1141 tgccggcgcg gcgggcaatt taatctatga agaagccacc cgggaaatcc gtacgcgcgt
     1201 catccaaggt ttgtctatga cttcggggat gcgtgcgacg gaactgttcc ccaatatgat
     1261 gttgcagatg tcttccatcg gcgaggaatc gggttctttg gacgatatgc tcaacaaagc
     1321 cgccgaattt tacgaagacg aggtggacaa tgcggtcggc aggctgtccg ctatgatgga
     1381 gccgatcatt atcgtgattt taggcttggt catcggcacg cttctggtcg ccatgtatct
     1441 gccgctgttt aatttgggca acgtggtcgc ctgatttgcc gcacagatcc ggcgcggatt
     1501 cgctctgcgc cggtttgttt ttgttttgaa tacattgaga acaaaatatg tctgatttgt
     1561 ctgtattgtc gccgtttgcc gtgcctttga cagcggtgtt cgggctgctg gtcggaagtt
     1621 tcttaaatgt cgtcatttac cgcgtgccgg tcatgatgga acgcggctgg acggtatttg
     1681 ccaaagaata tttaaacctg ccgctgaccg aagaggaaag ccgtaccttc aacctgatga
     1741 aaccggattc ctgctgtccc aaatgccgcg tgccgatacg cgcgtggcag aacatcccga
     1801 ttgtcagcta cctgctcctg cgcggcaaat gcgcctcctg ccaaaccaaa atcagcatac
     1861 gctatccctt aatcgaactg ctgaccggcg tattgttcgg gctggttgcc tggcaatacg
     1921 gctggtcttg gattacattg ggcggtttga tactgaccgc gtttctgatt tccctgacct
     1981 ttatcgatgc ggacacccaa tacctgcccg actcgatgac attacccttg atttggctgg
     2041 ggctgatatt taatttggac ggcggcttcg tgcctttgca gtctgccgtt ttaggtgcgg
     2101 ttgccggcta tggttcgata tggcttttat gcgcggtata taaactgatt aaaggcgaaa
     2161 tcggtatggg caacggagat ttcaaactga ttgccgcatt gggcgcgtgg ctcggcatat
     2221 ccgcattgcc cgtgctgatt tttgtttcct cgctgatcgg tttggtcgcg gcaatcgtta
     2281 tgcgcgtcgc caaggggcag cattttgcct tcggccccgc actgacagtt tcgggctgga
     2341 taatttttac ggcaaacgat tccgtatggc gggcggtcaa ctggtggctg acccatccgg
     2401 tgctgtaaga tgacggtatg ggtcggactg accggcggaa tcggcagcgg caaatcggca
     2461 gccgcgcaat gttttgccga tttgggcgta ccgcgtatcg atgcggatgc ggcggcgcac
     2521 tcgctgacgg cttcagacgg catcgccctg ccggaaatca ggcggctgtt cggcgacacc
     2581 gttttcgaca cacagggttt gttgcgacgc gacatattgc gtaaagaagt ctttgcctcc
     2641 ccatcgcgaa aagccttgct cgaatccgtg atgttgccgc tgattttctc agaaatcaaa
     2701 aaacagcaag aaacctttac cgatgcggtt tacggcattg tcgaaattcc gctgctgacg
     2761 gaaaagcgtc aatttatcag cctgatacgg cgtgtcctga ccataagtgc ccctgtggaa
     2821 aaacgtatcg gcagggtgat ggcccgcagc gggctgacgc gcggcgaggt ggcggccgtc
     2881 atcagccatc aggcatccga atccgaacgc ctgctgcttg cagacgatgt gctgctcaat
     2941 gacggcagcc tcaaaagcct gcgtgagaaa acaatgcgcc tgcacgcgtt ttattcaggg
     3001 attttcgcct caaaaccaac acaaggaaaa cacaatgact gaatcgcggc aaacacgcct
     3061 tcaagtcaaa tgtccgacct gtcaaacggc agtagtatgg aaaccggaaa acgcattccg
     3121 ccccttctgt tcgcaacgct gcaaactgat cgacttgggc ggatgggcag acgggaaata
     3181 tacggtttcc agccaaacgg aaagtttgcc ggaaatatcc gaacccgaca tggcataccg
     3241 ctgaccgccc cgccttcccg gcaaacaccc tgaaagtcaa atgccgtctg aaacaaacac
     3301 gcttcagacg gcattttcat tctcaaacct aatcgttggt atttgccgtt acctcttcca
     3361 atgaagtaat gccctgcata actttcaaaa taccggcccg gcgcaaatcc accataccct
     3421 ccttataggc aacgtccaaa atatccactt ccgtaccgtt gttcataatc acacgctgca
     3481 tttcttcgct gatgggcata acctcataca cgcccgcacg ccctttataa ccctgccccc
     3541 ggcaacggtc gcaaccgacg gcgcggtaaa gtttccaatc ttttgcaagg tcctcatcgg
     3601 tgaagccgac ttccttcaaa gcagaggcag acgggcgttc cacttcctgt ttgcagctcg
     3661 aacacagcct gcgtaaaaga cgctgcgcca taatcaggct gaccgaactg gcaatattaa
     3721 acggcgcgac acccatattc agcatacgcg acaacgtcgc cggcgcatta ttggtgtgca
     3781 gggtggaaaa caccatatgc cctgtttgtg ccgccttaat cgcaatatcg gcagtttcca
     3841 aatcacgaat ctcaccgacc ataatgatgt ccgggtcctg acgcaggaaa gacttcaaag
     3901 cagcggcaaa agtcaggccc tgcttatcat tgacgttaac ctgattgatg cccggcaggt
     3961 taatctcggc agggtcttcc gccgttgcaa tatttaccga ctccgtattc aaaatattca
     4021 aacaggtata gagcgacacc gtcttacccg aacccgtcgg accggttacc agcaccatcc
     4081 cgtagggacg gtgaatcgct tccaacaaca attttttctg aaacggctca aaaccgagct
     4141 ggtcgatgtt caaagacgcg gcatcggaat tcaaaatccg catcacgacc ttttcgccaa
     4201 acagcgtcgg caatgtgctg acacggaaat cgacaggctt gccgcccttt tgaaaggtca
     4261 gctgcatcct gccgtcctgc ggtatccgtt tttcggaaat gtccaaacgc gacattacct
     4321 taatccggga agcaagctgc ccccttaccg caatgggcgg ctgaaccacc tcgcggagct
     4381 gcccgtccac acggaaacgg atacgggcat tgtgttcgta aaactcgaaa tggatgtccg
     4441 atgccccgct gcgcaaggca tccgacaaag tcttatggat aaacctcgga acagggccgt
     4501 cttctgcctc ctcgttgtcg atatacaggg tgtggctttc ctcttcctcc tgcccctccc
     4561 caagctcctg aagcagcgat gtcgaacgcg aacccaccca atcgagcaaa cccgccaact
     4621 ggtcatcctc gacaatgacc aattcaacct caatccctgc ggcagaaacg gttttctgaa
     4681 tttgcggcat ctgtgtcgga tcggaaaccg caaaaaatac tttgtcgccc cgacggaaaa
     4741 ccggcacaca gtggaactcc accatctgct cctccgtcaa cacccccatc agcaccctgt
     4801 ggcgcggata atgacgcaaa tcaagaatcg aataactgaa caccctcgca atcaatgccg
     4861 caagcgactt gggcgaaatg acaccgtctg aaaacagcat cggcaacact tccttacccg
     4921 cctgcgactc attgtagtaa tgctcggcct gctcaacagt aaccacctgg ttttgaacca
     4981 gaatcctcag caaacctacg ctcatacgac cttatcccca aatttattca ttgttatacc
     5041 tgtacagc
//
LOCUS       DAWKOG010000147         4996 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.147, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000147 DAWKOG010000000
VERSION     DAWKOG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.147"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(391..825)
                     /locus_tag="WJK66_001759"
     CDS             complement(391..825)
                     /locus_tag="WJK66_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEZ7579428.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WJK66_001760"
     CDS             complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WJK66_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HEZ7579429.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            complement(1556..2398)
                     /locus_tag="WJK66_001761"
     CDS             complement(1556..2398)
                     /locus_tag="WJK66_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEZ7579430.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPVPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPGNASAKADSEQGNGE
                     DKGTGIKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            2653..3291
                     /locus_tag="WJK66_001762"
     CDS             2653..3291
                     /locus_tag="WJK66_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEZ7579431.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            complement(3743..4543)
                     /locus_tag="WJK66_001763"
     CDS             complement(3743..4543)
                     /locus_tag="WJK66_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEZ7579432.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTLAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
BASE COUNT         1289 a         1261 c         1072 g         1374 t
ORIGIN      
        1 ggtatagcgg attaacaaaa accagtacgg cgttgcctcg ccttaactca aagagaacga
       61 ttctctaagg tgctgaagca ccaagtgaat cggttccgta ctatttgtac tgtctacggc
      121 ttcgttgcct tgtcctgatt tttgttaatc cactataaaa attagaaatg cacattttca
      181 ttattctcgc gcaggcagga ctccagactt acccatttca gtaatgtttg aaaataaaag
      241 aaaaatcaga tgtttgtatt cccgcctgcg cagaaatgga gacggtgctc tgtcgtctca
      301 tttttgtttt aatcaactat atatagctga ttaaacataa gaaatgccgt ctgaaagact
      361 ttcagacggc attcgttcaa gcgtcgaact ttattgcgcc ttggtttcgg ttacaaaacc
      421 gattttggtg attcctgcct gacgggcggc ttctaaagct ttgtttacat aatcgtattc
      481 caccgccttg tctgccgcaa tcgccacaat cacgttttca ttctgctcct tggcggcttt
      541 cagacggctt tccacttccc cgatttccac tttgcttgca gaatccccgc cgacataata
      601 gccgccgttc gcatcaatcg tcaggcgcag ggggtcttta ggctgtttgt cctgcttgtt
      661 tgtctgctcg gacgcggtcg gcagttccaa agggatggaa tgcgtcagca ccggcatagt
      721 aatcataaac acaatcagca acaccagcat cacgtccacc aacggcgtaa cgttgatgtc
      781 ggacatcgga gaatcgtcgc cggaattcat cgaaccaaat gccataatca gctatccttt
      841 tgattaagca ggcggacgtg caaatcgtgc gccatcgcat ccaaatcctg ggtcagtatt
      901 tttgtgccgc gattgaggaa gttgtatgcc aacaccgccg gaatcgccac gaacaaaccc
      961 gccgccgtcg ccaccagtgc ctcgccaatc gggccggcaa ccgccgcaat actcatctgc
     1021 ccgctttgcc cgatattgat cagggcgtgg taaatccccc aaaccgtgcc gaacagcccg
     1081 ataaacggcg cggtcgcgcc gatggaggca agcgcggtca tcccgtaatc aaaccggcgc
     1141 ataatctgcg ccatactgtt gcggatttga atgaccaaat actcgttcaa cggcaaagcc
     1201 tgcgccagtt cggacgcttc gtttcggcgg tagttgcggt aagactgcaa tgcctcttgc
     1261 gccagtttgg acaaaggcgc atcgacggcg cgcacttttt cgaccgcgtc gttcagcgac
     1321 aaagtatcgc gcatatgccg tttgacggcg gcattccctt tgcgcgcccg atacagcttg
     1381 atgcagcgca agacaaccaa acaccacgtt acgatactca tcaacagcat caacacaaac
     1441 acaccgatca ggacgggatc gcccgattca aatactaatt tcaaattcat aatgattcca
     1501 acactgaaaa aaccaatcaa acatccaagc tgccgcaaac cgctgcggca accgcctaat
     1561 tcaattcaaa cttgacgggg actttaaact ccgtccaggc attggcttga aaatgcccgt
     1621 tttgcgccgc cttgcgtgcc gcattgtcca accgggaaaa accactgctt ttcacgattt
     1681 taacggactc aacatgaccg cccggagaaa ccaaaacgct caaaacaacc gtaccctgct
     1741 catcattctc catagaaagc gtgggataag ccgggcgcgg aatgctgccg ttggcgcgta
     1801 aaggattgcc tttgctgctg ccggctcctt ccccgtgttc gcctttgaca ccgccgctac
     1861 ctttgccgct gccttctccg cgccccgttc cgtctccttt gataccggtt cccttatctt
     1921 ccccattgcc ctgctcgctg tctgctttgg cagaagcatt gccgggatgt tcggcaggtt
     1981 tttcagacgg cttctcgacc ggtttttccg ccggtttcgg gacaggcttc gcttccggct
     2041 taggctctgg tttcggtttt tcttcgggtt tcggcttttc ttcaggtttc ggctcttcct
     2101 taggctgctg aatatccgca tccgcctttt tcgtaaccac cggcttcaaa accggcttgg
     2161 gcggctcgac aggtttgggc ggctcgggca cgggttgcgg ttcgggcgcg gcaggcgcgc
     2221 ctgcaccttc gggtgcgccg tcccctccgc caaaatcgcc caaatcgaca aattcaataa
     2281 cattgcctga ctctatcacg ggcagcttgt gcgcctgcca gagcaatgcc accattgcca
     2341 aatgcagcag tgcgacggaa aacacgactg cgggggttaa aattcgttct ttatccataa
     2401 ttcgggcata ataatagcaa caattcctat ttgcaaccta tttttacaat ttttggtcat
     2461 atgaatgtct gttccgttca caggcaaacc gtgtttaaac gctgtattac agcaaatcat
     2521 cagataacgg gccggcagaa aaaatgattc cgtctgattt cttattccaa taaaatcagg
     2581 ttagatgata tattgccgct tctgtctgtc agccgtttcg ggctgcacac cacatctgtt
     2641 caaaggaaaa ccatgtttca aaattttgat ttgggcgtgt ttctgcttgc cgtcctgccc
     2701 gtgctgctcg ccattaccgt cagggaggtg gcgcgcggct atacggcgcg ctactgggga
     2761 gacaacactg ccgaacaata cggcaggctg acactgaacc ccctgcccca tatcgatttg
     2821 gtcggcacaa tcatcgtacc gctgcttact ttgatgttta cgcccttcct gttcggctgg
     2881 gcgcgtccga ttcctatcga ttcgcgcaac ttccgcaacc cgcgccttgc ctggcgttgc
     2941 gttgccgcgt ccggcccgct gtcgaatctg gcgatggctg ttctgtgggg cgtggttttg
     3001 gtgctgactc cgtatgtcgg tggggcgtat cagatgccgt tggctcaaat ggcaaactac
     3061 ggtattctga tcaatgcgat tctgttcgcg ctcaacatca tccccatcct gccttgggac
     3121 ggcggcattt tcatcgacac cttcctgtcg gcgaaatatt cgcaagcgtt ccgcaaaatc
     3181 gaaccttatg ggacgtggat tatcctgctg cttatgctga ccggggtttt gggtgcgttt
     3241 attgcaccga ttgtgcggct ggtgattgcg tttgtgcaga tgttcgtctg actggctttc
     3301 agacggcata aacgctccag aaaacgcggc aggacatatt gccctgccgc gttttcctgt
     3361 agtgtaatct tatttttttc atcattatta gaaccaagtt gcatgataat acctttcatt
     3421 aactgaaaca ctgattaaga aactccagtc tgtctaatga tgaggttttc acatcgccaa
     3481 aacttgccaa tcaaatgctg gatttattgc cgtctgagat ttggtcaaat ccaaaggcga
     3541 cattcttaga ccctgtgtgt aaatcagggg tatttttgcg tgaaatcgtc aaacgcttgg
     3601 atgaaggctt gaccaatcaa ataccagata aacaaactcg cattaaccac attttaaaaa
     3661 atcaagtttt tggaagtact gccacgtatg taggtagctt tgaccgatat ttgcataaaa
     3721 actcctttgc tggtgaaagg aattattttg ccaattttaa aatatttcta gcaccaaata
     3781 gtacaatgac aaagacaatc atgccaatga ttaaatcagg atagctagaa tgagtcaata
     3841 acgtcaatgc tcccgccgct atcacaccga tattgatgat aatgtcattg gatgtaaaaa
     3901 tcatgctggc tttgatatgg atttctttat tttgattttt gctcagtaga tataagcaca
     3961 gccagtttgc aatcaatgcc aaaaatgccg tgccaatcat cagttgataa ttgggcagct
     4021 gctcagcacc gataaaacgc ctaatcactt ctatcacccc aaataacgcc aatattatct
     4081 gcgttatccc cgccaaaaat gccacacgtt ttttatacgc cagcgtcata ccaatggctg
     4141 atagcgccaa tatatagaca aagctgtccg ccagcatatc tagactatca gcaatcagcc
     4201 ccatagaatt agcaaaaata ccaaccgaac actctatgat aaaaaacaca aagttaatca
     4261 tgagcacttg atataataat cttttttcta agtgctcatc aggcttgtta aacactatct
     4321 tatcaacaat cacttcggtg gaaatgatat gactatcaaa attaagcggt tcaagtactt
     4381 gtaaaatcgt tgtatcttga ttatcgtgat agacggttaa gcaccgccca gcaatatcaa
     4441 actgtaattc ataaatatca gacacatctt ttaaacgcat gcgaatgagc tgttcttcgg
     4501 acgggcagtc cattttggta atgttaaaaa tggtcttttt catctattta gttccttgtt
     4561 ttgatcaggt tggctcaaat aaatctgtgt ttatattgct gcttggtaat ttttggatgg
     4621 tttgagtaaa ttgattaggt taaaatttac ctttggaagt accgccacgc ataatagttt
     4681 agatatgttt ataatctctg gataaaaaaa cgtaataagt gcttactgga taacaaagtc
     4741 caaaccaata gcagataaaa taaggcatcc accccctttc ttcattaagg ataagagata
     4801 tcgagaaaca aatcataact aaacagaaaa aattttggag ataaagccat ttcattcccc
     4861 tattcaagaa tctggccaag ataggtattt tgtatactct agaaaagaaa ggcatttcca
     4921 agggaaacat atcagataaa aacttttgtt tattttttac tatagataga accctatttc
     4981 tcaagataaa tccaaa
//
LOCUS       DAWKOG010000148         4977 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.148, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000148 DAWKOG010000000
VERSION     DAWKOG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.148"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(210..2486)
                     /locus_tag="WJK66_001764"
     CDS             complement(210..2486)
                     /locus_tag="WJK66_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ7579433.1"
                     /translation="MAQTTLKPIVLSILLISTPILAQAHETEQSVDLDEVTVVGKSRP
                     RATSGLLHTSTASDKILSGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGELGLRLSSGNLEKLTSGGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGVAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDDDDAHAHTHSGRPWIDLRNKR
                     YELRAEWKQPFPGFEALRVHLNRNDYRHDEKAGDAVENFFNNQTQNARIELRHQPIGR
                     LKGSWGVQYLQQKSSALSAISEAVKQPMLLDNKVQHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIQYDKALIDRENYYNHPLPDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVPAARLGFHLKASLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            2906..3496
                     /locus_tag="WJK66_001765"
     CDS             2906..3496
                     /locus_tag="WJK66_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEZ7579434.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIDRNTMPECLEVTAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLIEFQNFKPQKI"
     gene            3560..4618
                     /gene="trpD"
                     /locus_tag="WJK66_001766"
     CDS             3560..4618
                     /gene="trpD"
                     /locus_tag="WJK66_001766"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEZ7579435.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQMMSGKVPPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVMEQMGANLNLTPEQIAQSISQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLDGKEGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(4770..>4977)
                     /locus_tag="WJK66_001767"
     CDS             complement(4770..>4977)
                     /locus_tag="WJK66_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ7579436.1"
                     /translation="TVVVPNAQSATLLPIIRKKVKPDGIVYTDTFRSYDVLDISEFSH
                     LRKFNGIPKEHLGLHLKKCQWHFK"
BASE COUNT         1143 a         1143 c         1409 g         1282 t
ORIGIN      
        1 ggaatgacgg gatcttgggt ttctgctttt gatttttctg cttttgcgag aatgacggcg
       61 tgaaagtaag aatgatgaaa caaaaaaaat gggaatgatg gcatagtggt ttgttctttg
      121 tctttgccat atttcctaac aaattgatta aaaagaaaaa aggttttcag aatgccgtct
      181 gaaaaccttt tttgtttgcc tgtccgattt taaaacttca cgttcacgcc gccggtaaag
      241 ctgcggccca tttgcggcgt atcagagaga aagctgctgt gggcgtaaac ggattggttg
      301 agcaggttgt cggctttgac gtaccaattc cactcgccat agcgcgtatt gcggcggtag
      361 tttgcgccga ggttgagcat atggtgtccg ggcgtgcgcg tttcgtagcg ggcgagtttg
      421 ttttgggcga acacgcggta gtagtccaaa ttggcatcga tacggtcggt cagcgaggct
      481 ttcaggtgga agccgaggcg cgcagccgga acacgggggg cattttggtc gtcctgtgcg
      541 atgaaaggac ggttgccgta ggcatcttct ctgccgggta gggaaggcag gtttttcaga
      601 cggcctcgta catagtcgcc ggaaacgccg atgcggtagc gcggtgtcgg tttgaagtag
      661 atttcgcctt ccgcgccgta gaagtcggcg ccggattggt tgtagcgcac gagcttcatt
      721 tcgctgtcgt cttcgatgga tttggggccg cgtccgtcgt ttaaggtttg ggcgtaaatg
      781 tagttaccga agcggttgcg gtagagtgcc agattgtatt gccagcggtc gccttcgtag
      841 cccagcgcga gttcgatatt gttggaacgc tctttgttga ggtgtttgtt gccgacttca
      901 aaggtgttgg tggcgacgtg tttgccgtgt gcgtacagct cttgcgttga cggcaggcgt
      961 tcctgatggg aggcggtcag gctgagtttg tgttgtggcg tgaaatacca gttgcccgaa
     1021 agtgcgaatg agcgggcggt ttggcggtgc gcgccgaggt cgggcagggg gtggttgtag
     1081 tagttttccc gatcaatcaa tgctttgtcg tactgaatgg aggctttttg tttttccacg
     1141 cgtacgccgc cttcaagcgt gaagttgtcc cagtttgcct gttctacacc gaaaaagctg
     1201 taatgttgca ctttgttgtc aagcagcatc ggttgtttaa ccgcttcgga tatggcagat
     1261 aaagcactgg atttttgttg taaatattgc acgccccagc tgcctttcag acgacctatg
     1321 ggttggtggc gcaactcgat gcgggcgttt tgcgtttggt tgttaaaaaa gttttcgact
     1381 gcatcgcctg ctttttcgtc gtggcggtag tcgttgcggt tcaggtgtac gcgcagggct
     1441 tcaaaaccgg ggaacggttg cttccattcg gcacggagtt cgtagcgttt gttgcgcagg
     1501 tctatccacg gtctgccgct gtgggtgtgt gcgtgtgcat catcgtcgtc gtggaagccg
     1561 cagctcaagc ccggattgtc gtaatcgatg tcttcttcgg tcaacaggtg cggataaagc
     1621 tgtaaatagc gtttgttaat caagctcttt tgccagatga tgtcggcgtg gcaatcatcg
     1681 tattcgtggc tgtgggcagg cagaccatat tggtcgcgac ggtcgctgta cgctacgccg
     1741 ataaaacctt tttcgccaac ccaagacagc ccgatgctgc ccgtttgcga atcggcgtgg
     1801 ctgtcgggca ggcgtttcag attgcggtaa cgcggtacgg cgtaatcccc cgatttgcgg
     1861 tacagccctt ccgtgtgcaa tacaaagttt ttgcccaaac cgatattgat gccgccggac
     1921 gtgagttttt ccagattgcc gctgctcaaa cgcaatccga gttcgcccga tacgccgttt
     1981 tcaggcattt tttcggggat tttgccatcg gcaacatcga ccagccccgc cacattgccc
     2041 gagctgtaca agagcgtaac cggcccgcgc aggatttcga cctgttgcga caaggcggta
     2101 tctaccataa tggcgtgatc gggcgaaaaa tccgccatat cgcccgtttc gccgtgatgg
     2161 ttcaacactt taatccgcct gcctgtttga ccgcgaataa cgggagcgga cgcgccgccg
     2221 ccgtattgcg aggcgtgaat gcccggcacg ccgtccaaag catcgcccaa gttgacggct
     2281 ttttggcgca aggtatcgcc ggagaggatt ttgtcggaag cggtcgaagt gtgcaacagc
     2341 ccggatgtgg cgcgcggacg gcttttgccg acgacggtaa cctcgtccaa gtcaacagac
     2401 tgctcggttt catgcgcttg ggcgaggatg ggggtgctga ttaaaagaat tgataaaaca
     2461 atgggtttga gtgtagtttg tgccattttg gcttctcgtc gcatttcaaa agtttgttat
     2521 tatataacat tacatttttt atatcataag attttgagaa cactcagagg gcataggcaa
     2581 aagtttttca aatgaaacgg ttgcggcatc gggcggtgtc catttgtatc cgccgtcatt
     2641 cgggggcgcg gttgatgttg acgcagattc cgctgtgttt gcccactatg ttcggctgcg
     2701 ggataaccaa aatttatgag tgcataaaaa cggcacgttc ccgaacggtc gaaaaggtgg
     2761 caaaatggcg tacttgtcag aacgcgaggc tctgcccagg ttcacgaggg cgcatcgggc
     2821 acgctaagat ataaggggta tggatggtgg tatcggaaat gcagttaata acaaacaaat
     2881 tataaatcaa taggttaatc acaaaatgct tttgtttatc gacaattacg acagttttac
     2941 ttacaacatc gtccagtatt tcgctgaatt ggggcaggaa gtcgccgtgc gccgcaacga
     3001 tgatattacg ttggaagaaa tcgaggcatt gaatccgcaa tatctcgtta tcggacccgg
     3061 cccgtgttcg cccaaagaag cggggatttc cgtggcggcg atgcgccatt ttgccggccg
     3121 gctgccgatt atgggcgtgt gcctcgggca tcagacgata ggcgaggcgt tcggcggcag
     3181 gatagtccgc gccaaaacgc tgatgcacgg taaggtgtcg cccgtgtccc attcgggcaa
     3241 gggtatgttt aagggtttgc ccaatccggt tacctgtacg cgttatcaca gcctcgttat
     3301 cgatcggaat acgatgcccg aatgtttgga agtaacggct tggactgagg acggcgagat
     3361 tatgggtgtg cgccataagg aatatgccgt cgagggcgtg cagttccacc ccgaagccct
     3421 cttgaccgag cacggacatg atatgttaaa caatttttta atcgaatttc aaaacttcaa
     3481 accgcaaaaa atctgacgtg atgccgtctg aagcccttca gacggcattt tcgtccgaat
     3541 attgaacgga ggacaaaaaa tgattacacc gcaacaggct atcgaacgat taatcagcaa
     3601 taatgagttg ttttacgatg aaatgaccga cttgatgcgc caaatgatga gcggaaaagt
     3661 gccgcccgaa caaattgcgg cgattttgac aggattgcgt atcaaggttg aaaccgtttc
     3721 cgaaattacc gcagctgcag ccgtcatgcg cgagtttgcg acaaaagtgc cgctggagaa
     3781 tgcggagggg ctggttgata tcgtcggtac gggcggggat ggtgccaaaa ccttcaatat
     3841 ttcgacgact tcgatgtttg ttgccgcagc ggcaggcgcg aaggttgcca aacacggcgg
     3901 gcgttccgtt tcatcttcca gcggcgcggc ggacgtgatg gagcagatgg gcgcaaacct
     3961 caacctgact cccgaacaga ttgcccaaag tatcagccag accggtatag ggtttatgtt
     4021 cgcccccaat caccacagtg ccatgcgcca tgtcgccccg gtacgccgtt cgctcggttt
     4081 ccgaagcatt ttcaacatat tgggtccgtt gaccaatcct gcgggcgcgc cgaaccagct
     4141 tttgggcgtg ttccacaccg atttgtgcgg cattttgtcg cgggtcttgc aacaactcgg
     4201 ttcaaaacac gttttagtcg tttgcgggga gggcggtttg gatgaaatta cactgacggg
     4261 caaaacgcgc gttgccgaac tcaaagacgg aaaaatcagc gaatacgaca tccgcccaga
     4321 agatttcggt atcgaaaccc gccgcaattt ggatgaaatc aaagttgcca atactcagga
     4381 atctttgttg aaaatgaatg aggtgctgga tgggaaagaa ggggctgcgc gcgatatcgt
     4441 attgcttaat accgccgccg ccctatatgc cggaaatgtc gctgcttcgc tttcagacgg
     4501 catatctgcc gcacgggaag ccatcgattc aggcagggcc aaatcgaaaa aagaggagtt
     4561 tgtcggtttt caaccacaac aaagatgcca ttttcttgga aagatggagc ttgggtgatg
     4621 ccgccatgat tatggaactt ttgtggcaaa acataagcac ttcacgaaga gaacttacca
     4681 aactgttttt atataaaaac ttggggctgt actagataac cagaccaaat tcccattaac
     4741 taattgtctt aaaatctgaa tttgagattc tatttaaaat gccattggca tttctttaaa
     4801 tgcagcccca aatgctcttt gggaatgccg ttaaacttac gtaaatggct aaattcacta
     4861 atatcaagca catcataact acgaaaggta tccgtgtata caatgccatc aggcttaact
     4921 ttctttcgga taattggcaa caatgtcgct gattgcgcat taggaacaac gacggta
//
LOCUS       DAWKOG010000149         4829 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.149, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000149 DAWKOG010000000
VERSION     DAWKOG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.149"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            245..982
                     /locus_tag="WJK66_001768"
     CDS             245..982
                     /locus_tag="WJK66_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEZ7579437.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDSGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWPSEKIEARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALASKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHARSDIGRELIRED"
     gene            984..1670
                     /locus_tag="WJK66_001769"
     CDS             984..1670
                     /locus_tag="WJK66_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ7579438.1"
                     /translation="MADLTFQQAVSNIIGMKGEIVRALGETFVMVGLSTTFAVVFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPIA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPLAIVRKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            1829..2692
                     /locus_tag="WJK66_001770"
     CDS             1829..2692
                     /locus_tag="WJK66_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579439.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAAKKE
                     IVFGTTVGDFGDMVKEQIQAELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPAAWNEGAAK"
     gene            complement(2755..3324)
                     /locus_tag="WJK66_001771"
     CDS             complement(2755..3324)
                     /locus_tag="WJK66_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEZ7579440.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSTEWQGMAD"
     gene            3482..4342
                     /locus_tag="WJK66_001772"
     CDS             3482..4342
                     /locus_tag="WJK66_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEZ7579441.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGRIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            4477..4815
                     /locus_tag="WJK66_001773"
     CDS             4477..4815
                     /locus_tag="WJK66_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579442.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRSNEGIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGRC"
BASE COUNT         1194 a         1457 c         1189 g          989 t
ORIGIN      
        1 tttcaattat ttgccgtttt ggtgcgaacc gctgcctttg cccgtttcag acggcattgt
       61 ccgaaatggt tgcctgcttc ctgccttatt gacaaaaaaa tgctttcccg ataatatcct
      121 acgaaaatta acctgccgat ttgacacagc ttgcgggcat aacagctaaa gcgttccgac
      181 aattccagct ttatcttccg cgcccgttgt gtccgacatc gggctttgtt gtatgggaaa
      241 gacaatgatt attttggaca aggtttccaa gcattaccaa acgcgcgaca agacccgttt
      301 tgccgctgtc gagccgacca gcctcgaaat ccgcgacggt gaaatcttcg ggctgatggg
      361 ttattcgggt gcaggcaaat ccaccctgtt gcgcctgatt aacctgttgg aacgtcccga
      421 cagcggcaag gtcaacgtct gcggacaaga gctgaccgcg ctcgatgccg ccgcattgcg
      481 tcaggctcgg cagaatatcg gcatggtgtt tcagcagttc aacctgttga acaaccgcac
      541 cgtcgccggc aacgtcgcct tcccattgga aatcgccgga tggccgtctg aaaaaatcga
      601 agcgcgcgtt gccgaatgtc tcgaaatcgt cggactgacc gaacgcgccg gtcactatcc
      661 cgcccagctt tccggcggac aaaaacagcg cgtcggcatc gcccgcgcgc tcgcatccaa
      721 gccgcaagtc atcctcgccg acgaacccac ttccgccctc gaccccgcca ccacgcgcag
      781 cgtcttggaa tgtttggaag acatcaacaa acgcttcaac gtaaccatcg tcatcgtaac
      841 ccacgaaatg agcgtcatca gacgactgtg cgaccgcgcc gccctgttgg ataagggaaa
      901 agtcgtcgaa atcgtcgaag tacgcggcaa ccaaatccac gcccgatccg acatcgggcg
      961 cgaactcatc agagaggact gaaatggcag acctgacctt ccaacaagcc gtttccaaca
     1021 tcatcggtat gaaaggcgaa atcgtccgcg cattgggcga aaccttcgtg atggtcggct
     1081 tgtccaccac attcgccgtc gttttcggca cgttgctggg cgtgttgctc ttcgtaactt
     1141 ccagccgcca actgcattac aacaagccgg tgaacttcct gctcgacaac ctcgtcaacc
     1201 tcatgcgcgc cttccccttc gtcattttga tgattgcgat gatacccgcc acacgcgcca
     1261 tcgtcggcag caccatcggc ccgattgccg cctcgctggt gttgagcgtg tcgggattgt
     1321 tttattttgc ccgactggtg gaacaaaacc tgcgcgaagt ccccaaaggc gtgattgaag
     1381 ccgccgccgc gatgggtgcg ccgccgcttg ccatcgtccg caaagtcctc ttaaacgaag
     1441 cgcgcgcggg tatggtttcc agcattaccg tgcttgccat cgggcttttg tcatacagcg
     1501 cggcggcagg gatgataggc ggcggcggct tgggcgacct cgccatccgc tacggctact
     1561 accgctacca aaccgaagtc atcgtcttca tcgtcgccct cctcgtgctg ctggtcatcc
     1621 tgattcaaag caccggcaac gcgttggcgc ggaaactcga caaacgttga acccgaatgc
     1681 cgtctgaacg ccaaaacccc caccgctatc cgaaaaatgc tataaaatcc ccctgttcgc
     1741 ggcaaatgcc gtctgaacgc cgaatccgga cggcaggact ccctgcccgt catttttgtt
     1801 tgaaactgcc acaacatcag gagaaaatat gaaaaccttc ttcaaaaccc tttccgccgc
     1861 cgcactcgcg ctcatcctcg ccgcctgcgg cggtcaaaaa gacagcgcgc ccgccgcatc
     1921 cgcttctgcc gccgccgaca acggcgcggc gaaaaaagaa atcgtcttcg gcacgaccgt
     1981 cggcgacttc ggcgatatgg tcaaagaaca aatccaagcc gagctggaga aaaaaggcta
     2041 caccgtcaaa ctggtcgagt ttaccgacta tgtgcgcccg aatctggcat tggctgaggg
     2101 cgagttggac atcaacgtct tccaacacaa accctatctt gacgacttca aaaaagaaca
     2161 caatctggac atcaccgaag tcttccaagt gccgaccgcg cctttgggac tgtacccggg
     2221 caagctgaaa tcgctggaag aagtcaaaga cggcagcacc gtatccgcgc ccaacgaccc
     2281 gtccaacttc gcccgcgtct tggtgatgct cgacgaactg ggttggatca aactcaaaga
     2341 cggcatcaat ccgctgaccg catccaaagc ggacattgcc gaaaacctga aaaacatcaa
     2401 aatcgtcgag cttgaagccg cgcaactgcc gcgtagccgc gccgacgtgg attttgccgt
     2461 cgtcaacggc aactacgcca taagcagcgg catgaagctg accgaagccc tgttccaaga
     2521 accgagcttt gcctatgtca actggtctgc cgtcaaaacc gccgacaaag acagccaatg
     2581 gcttaaagac gtaaccgagg cctataactc cgacgcgttc aaagcctacg cgcacaaacg
     2641 cttcgagggc tacaaatccc ctgccgcatg gaatgaaggc gcagccaaat aaggcagtcg
     2701 tataaaatga tgccgtctga accgcattcc gtgttcagac ggcatttttg tcctttaatc
     2761 cgccattccc tgccattccg tcgaatccgg tgtatcgatt ccgaacagcg acaaagcgtg
     2821 tgcaacactg tgcgccacta tgtcgtccgc cgtctgcggt ttgcggtaca tcgcaggaac
     2881 agggggaaac accacgccgc ccatttccgt tacccgcttc atattgtcca aatgggcaag
     2941 gttcagcggc gtttcgcgca ccatcagcac cagccgccgc ctttccttca aaaccacatc
     3001 cgccgcacgc gtcaagaggt tgtcgccgaa gccgtgcgcg accgaggcaa gtgtccgcat
     3061 cgaacagggg gcgaccagca tcccgtccgt tttaaacgta ccgctggcaa tgcacgcccc
     3121 gatattgccg atcggatgca cgaagtccgc caaggcatat acctcatctc tcgcataagc
     3181 cgtttccgaa gcgcgcgcca tctccgcacc tttcgatacc acaaggtgcg tttcgatatc
     3241 ttgcgcgcgt aaaagttcca aagccttcac gccgtattgg aaaccgctcg ccccactgat
     3301 gccgattatc aaacgccgta ccatcatccg cctttcccat aaaaccgcct gcaacggcaa
     3361 accggctatt atagtgaaaa aacagaaatc cgataaatac ggatacaaat tgtcggcaac
     3421 acccaatatc cgataaaata tccgatttaa catcctatct gaataggcac gggagggcgg
     3481 tatggcaaaa gtaaaaggcg gattggggcg cggtttggat tcgctgctcg ccaacggcgc
     3541 agacaacagc agcggcgacc gattgaccac ggttgcggtt aaagatatcc ggcccggccg
     3601 ctatcaggcg cgtgttcaaa tcgatgacga agccttgcag gagctggcag attcgattaa
     3661 ggcgcaaggc gtgatacagc ccgtcatcgt gcgcgaacac ggactgtccc gatacgaact
     3721 gattgcgggg gagcggcgtt ggcgtgccgc acagattgcc ggcctgaccg aaatccccgc
     3781 cgttatcaaa accatcagcg acgaaaccgc gttggcaatg ggtttgatcg aaaacctcca
     3841 gcgcgaaaac ctcaacccca tcgaagaagc acaaggcttg aaacgccttg ccgacgagtt
     3901 cgggctgacc cacgaaacca tcgcccaagc cgtcggtaaa agccgaagcg cgatttccaa
     3961 cagcctgcgc cttttaagcc tgcccgaacc cgtgcaggaa atgctttacc aacgccgcct
     4021 cgaaatgggg cacgcccgtg cactgctgac cctgcccgtc gtcgaacagc tcgaattggc
     4081 gcaaaaggct gtcaaaaacg gctggtcggt gcgcgaagtc gaacgccgca gccaagccgc
     4141 ccttcaaaac aaacgtcccg agcccaaaaa aactgccgcc gccgacatcg gccgcctgaa
     4201 tgatttgctg actgaaaaac tgggtgtcaa cgccgaaatc aaaaccgcca accataaaaa
     4261 aggcagaatc atcctgcatt tcgacacgcc cgaaaccttc gaccatatct tgaagcagtt
     4321 gggcatagat taccgacctt aattttgcgg gatataccgt ctgaaatata gagaatagct
     4381 ttccagattt taagtgggaa atataattct attgacattt ttctgcttca cgtaagaatc
     4441 gttttcctgt tttcattttt aattttcgag gaaattatga acacacgcat catcgtttcg
     4501 gctgcgttcg ttgcgttggc attagcaggt tgcggctcaa tcaataatgt aaccgtttcc
     4561 gaccagaaac ttcaggaacg tgccgcgttt gccttgggcg tcagcccaaa tgccgtaaaa
     4621 atcagcaacc gcagcaatga aggcatacgc atcaacttta ccgcaactgt gggtaagcgc
     4681 gtgagccaat gctatgttac cagtgtaatc agcacaatcg gcgttaccac ttccgatgca
     4741 atttgtttgg gaggcggaac gcacaaaggc aaaagtcaat gcaatgcttt gcttaaagcg
     4801 gcaggccgtt gctaatcctt tattcggaa
//
LOCUS       DAWKOG010000150         4823 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.150, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000150 DAWKOG010000000
VERSION     DAWKOG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.150"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(72..554)
                     /locus_tag="WJK66_001774"
     CDS             complement(72..554)
                     /locus_tag="WJK66_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ7579443.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
     gene            complement(707..868)
                     /locus_tag="WJK66_001775"
     CDS             complement(707..868)
                     /locus_tag="WJK66_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579444.1"
                     /translation="MSGQPEKHHTSPIEDKRKNPVYRMGRAVAGFMLVVWVAVLALVF
                     FLVFRFWLS"
     gene            complement(942..1403)
                     /gene="recX"
                     /locus_tag="WJK66_001776"
     CDS             complement(942..1403)
                     /gene="recX"
                     /locus_tag="WJK66_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEZ7579445.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKQAA
                     IAVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            complement(1476..2186)
                     /locus_tag="WJK66_001777"
     CDS             complement(1476..2186)
                     /locus_tag="WJK66_001777"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEZ7579446.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLSNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETA
                     TGLALLKSLGIPLAVITNKNEILAVELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKDEE"
     gene            2593..3861
                     /locus_tag="WJK66_001778"
     CDS             2593..3861
                     /locus_tag="WJK66_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEZ7579447.1"
                     /translation="MSSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRV
                     LEVNFPVKLDDGTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCC
                     VAGIPYGGGKGGITLDPRDYSEAELERIARAYSEAISPLIGEKIDIPAPDVNTNGKIM
                     SWMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGA
                     TYAIQGFGNVGYHTGYYAHQSGAKVVAVSTVDVAIYNENGLDMEALFKEFQEKGFITN
                     EAGYGKEITNAELLALDVDVLAPCALENQLTSENAGKVRAKIVVEGANGPTTPEADVI
                     LRQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNL
                     AQEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            complement(3919..4725)
                     /locus_tag="WJK66_001779"
     CDS             complement(3919..4725)
                     /locus_tag="WJK66_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEZ7579448.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
BASE COUNT         1034 a         1090 c         1428 g         1271 t
ORIGIN      
        1 cttgatttca tccgtttgca atggaaacgg atggcgcgat gtgcctgtct ttcagacggc
       61 atagtccgcc gtcagcagcc gcaggacatg tcttcagagg cttgcaggct gatgtctctg
      121 cgttttttgg ctttttcgta gcggcagcgt tggcggtcgg tcaggatttc cagcggaggg
      181 acagggacgg gtttgccgtc tttgaccgca accatcgtga agtagcagct gttggtatgg
      241 cggatttcgc cggtgcggat gttttgtgct tcgacacgga tgccgatttc catcgaggtg
      301 cgtcccgtgt agttgacagc ggcgtaaaag gtaaccaaat cgccgatgtg gatgggttct
      361 ttaaacagga ctttgtcaac cgacagggta acgcaataat tgccgctgta acggctggcg
      421 caggaatagg cgacttggtc gagcaggagc aggagttcgc cgccgtgtac gttgccgctg
      481 aaattggcgg tgtccggcat catcagttcg gacataatga gttcgtgcga aggcagttgg
      541 cgttgttgtg tcatggtgta cctgttttaa gaaagggtgt tttgaaatgg gtgcggggaa
      601 attttaggga atggggaagt ggaattcaag ctaaaaaaag ctatggcggg atagtctgca
      661 gaaatgggcc gccgatgccg tctgaagcct tcagacggca ttttgtttag gaaagccaaa
      721 aacggaagac caggaaaaac acgagtgcca atacggcgac ccaaacgacg agcatgaatc
      781 cggcaactgc ccgtcccata cggtaaaccg ggtttttgcg tttgtcttcg atgggggagg
      841 tgtggtgttt ttcgggttgt ccggacattt tgttctcaat cggaaaagtt ggaaacgggc
      901 gtaaggttat gttgccgtgc aaaaagattc aagaaggaag gtcagcagcc gtcttcccaa
      961 ttttcgtccc aagcgtgttt caatgccgtc tgaacggtat cggcatcgaa tccgcgatag
     1021 gcgaggaaac gcgcctgttt ttgtttttct ttgaggtcgg cggcggggtg tttgaatttt
     1081 ttacgcaaca cggctatggc ggcctgtttt tcgcttgagc ggtcgggaag caggttgcgg
     1141 ctggtttctt catctatgcc ctgttgcgcc aaagcctgtt tcagcctcaa tgaaccgtgt
     1201 ttgcggcttt tgctgcggat ataggcttcg gcatagcgca aatccgactg ccagttgcgt
     1261 tcggcaaatt cgtttaacac gttttccaac tcctcttcgc tttcggcgtg cggtgcaagt
     1321 ttgcgtttca gaccgatgcg gctgacttct tggcgcgaga ggatgtccat cgcgcgggcg
     1381 cgtagggatt tttgcggttt catggcggcg gtttcggcac ggaaagattc agacggcatg
     1441 ggaaacggat tccgatgccg tctgaacaga ttgttttatt cttcgtcttt atttttctgc
     1501 ggttgcaggt tttcgtaaat ttcgggcagc gagccgataa tccagtcggg gcgggtcgca
     1561 tcgtcttgcg agagcagcgt catatcgccg taaccgaagg taacgccgac gctcaggcag
     1621 ccggcggctt tggcggcgat gatgtcgttg cgcgagtcgc cgaccataac catgtttgca
     1681 acatcgatac ccaaaacttc ggcggcgtgc cgcagcggca gggggctggg ttttttctcg
     1741 ggcaggctgt cgccgccgag tatcaggctg aagtagtcgg caagtccgag ctgtttcaac
     1801 agttcgacgg caaggatttc gtttttattg gtaatgactg ccagcgggat gcccaaagat
     1861 ttgagcaatg ccagcccggt tgcggtttcg ggataggggc gggtgaagac gctcaaatgg
     1921 tcgcggtagt atttcatata ggatacgaaa cctttttccc acagttcgga atcggcttcg
     1981 cggtcgcggt cgttggagag gacgcggtga accagtttgc cgatgccgtc gcccacatag
     2041 ctttcgacca ctttggcggg cagcggtttc ataccgagtt gttccaacat cgcttctgcg
     2101 gcggcggcaa ggtcggggac ggaatcgcac agtgtgccgt ccaaatcgaa ggcgacggct
     2161 tggacgtgtt cgatggctgc attcataatc tgatttcctg tcgggttgaa agggtttatt
     2221 ttaacatcgt ttgcacaggg cttctaatgt atagattgtt aacaatcgca acaggttttg
     2281 caggcggtgt tttcggattt cggggcggaa tcgggcaagg gtttgaaatc ccagcgatgt
     2341 tccaattaaa tttttaaaaa ataaaaggat gcctatatct ttatttaatt tgacatttat
     2401 ttttcggacg cgcgcgctgc tttttcaggc ggagtccggg ctgattccgt gcggcttgaa
     2461 aaataaaatc atatatagat taatcaaatc gggcttatgc cgactacacg ctattacatt
     2521 ttgtcgggaa attggttata gtaatgagcg aaatgcacgc ccctgaaaat ctttttgtga
     2581 aaaggaagca aaatgtcgtc tgaagccctt gcaaaagaaa ccctcaatcc gtttgaaatc
     2641 gcgcgcaaac aggtcaaaac cgcctgcgac cggctgaaaa ccgatccggc cgtttatgaa
     2701 atcctgaaga gtccgacccg cgtgttggaa gtcaatttcc ccgtcaaact cgacgacggc
     2761 acggtcaaaa ccttcacggg ctaccgttcg caacacaaca acgccgtcgg cccctataaa
     2821 ggcggcgtgc gcttccatcc cggagtgaat ctggacgaag tcaaagccct gtcgatttgg
     2881 atgaccatca aatgctgcgt tgcgggtatt ccttacggcg gcggcaaagg cggtattacc
     2941 ttggatccgc gcgattattc cgaggcggaa ctggaacgca tcgctcgcgc ttattccgaa
     3001 gccatttctc cgctgattgg cgagaaaatc gatattcccg cccccgatgt gaataccaac
     3061 ggcaaaatca tgtcttggat ggtggatgct tatgaaaacg tggtgaaaca ttccgcgccg
     3121 ggcgtattta ccggaaaacc ggttgagttc ggcggctctt tggcgcgtac cgaggctacc
     3181 ggttacggcg tgaacctcgc cgccgtccaa gctttggaaa aactgggcaa ggacgtgaag
     3241 ggtgccactt acgccattca aggtttcggt aatgtgggtt accacacagg ttattacgcg
     3301 catcaatccg gcgcgaaagt tgttgcggtt tctactgtcg atgttgccat ttacaacgaa
     3361 aacggcttgg atatggaagc tttgtttaaa gagttccaag aaaaaggctt catcaccaac
     3421 gaagccggtt acggcaaaga aattaccaac gccgaacttt tggctttgga tgtggacgta
     3481 ctcgcccctt gcgcgctgga aaaccaactg acttccgaaa acgccggtaa agtccgcgcg
     3541 aaaatcgtgg tcgaaggcgc gaacggcccg actacgcccg aagccgacgt tatcctgcgt
     3601 caaaacggcg tattggtcgt gcctgatatt ttggcgaact gcggcggcgt ggtggtatct
     3661 tattttgaat gggtacaaaa cctgcaaggc tattattggg agtttgacga agttcaagag
     3721 aaagaaaccg tcgtcctgcg acgcgcgttc cgcgatatat ggaacttggc tcaagaatac
     3781 gatgtcgatt tgcgaaccgc gtcttacatg atgagtatcc gccgtgttga aaaagcgatg
     3841 aagctgcgcg gttggtattg atgtttgaac aaaaaatgcc gtctgaagcc ttcagacggc
     3901 atttttgtgc gggcgggatt aatggtgcga atggccgatt tggaaggtta aagtcgagta
     3961 gttcgcctgt ttttggcaca cgctttgatc ggggaagtcg gctttgtgtt cgacattggc
     4021 tttccagaag ccttggcgca aggggatgat gtccacttcg cctttgtcgt ctgtgctgtc
     4081 ggagaaagcc tgtgcttcgg ttttgtgcgt tttgctgcgg tcgctggtgt cgaagccgtc
     4141 aaaggtggcg gtaacggtgg cattgggcag cggttcgcca cggaacagaa cgcggacttt
     4201 gaagcgttcg cctacgtgaa tgttggcggg attgtccagc gggacgattt ccaagttttg
     4261 tccgaccggt ttggtgatga tggcggtgtc cgcgctttcg tgtccgacgt tgacgatgtt
     4321 tttgccgaac attcgggttt gttcgcaata gcttgcgtca ggcatttctt tgatgcccgc
     4381 ctgtttccag cctgctttgt tttttgacca gaaagtaggc tgatattcgg cgatgacgag
     4441 gtaactgccg tccttaacgg gacggttgct tcggtactgg tagttgtatg tgccgcgttg
     4501 aatcatgttt tccttgcctt tttcggtaac cagctgcatc ggtttgctga agatgtgcag
     4561 gcggtctttg gcgatgggtt cgagttcggg aaattcgccg tagcccaagt cggctttaag
     4621 gtattcgccg ccgtgcgtgt gggcggtttc gacccagacg cggtgggcgt gggcggatgt
     4681 ggcaaatagg gcggaaacgg cgagcagtgt caatgttttc ttcatggtgc agcctttgtc
     4741 tcgtgtgggt taaagatttt gttatgttat aacaaaatag gtatcgtgtg aagatggatt
     4801 tgctgtctga aaactgtctt ttt
//
LOCUS       DAWKOG010000151         4769 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.151, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000151 DAWKOG010000000
VERSION     DAWKOG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.151"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(1019..1456)
                     /gene="rnhA"
                     /locus_tag="WJK66_001780"
     CDS             complement(1019..1456)
                     /gene="rnhA"
                     /locus_tag="WJK66_001780"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEZ7579449.1"
                     /translation="MDKTVYLYTDGACKGNPGAGGWGVLMRYGSREKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWKELDVLVGQHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
     gene            1570..2439
                     /gene="tehB"
                     /locus_tag="WJK66_001781"
     CDS             1570..2439
                     /gene="tehB"
                     /locus_tag="WJK66_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEZ7579450.1"
                     /translation="MKEWIVGQSGELFCFGQMPVWKAENLPEVLLSGYSSEGGEWVCL
                     NVLQGDVEVRTQDGAAEVWSAESGDCVFAPQQVFSVKPKTDDAEIRLSMYCVAEDYFH
                     KKYGMSATHSAVAAAQDIVPAGRALDMGCGQGRNALFLGLKGFEVTAVDHNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGAMHAKDENGNPI
                     RFKFVTMLAKRPE"
     gene            complement(2489..3295)
                     /gene="thiD"
                     /locus_tag="WJK66_001782"
     CDS             complement(2489..3295)
                     /gene="thiD"
                     /locus_tag="WJK66_001782"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEZ7579451.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCDFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSSSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGLDVCKAVRTAKSYITAAISSPLEIGAGHGPVNHWAYRD"
     gene            3356..3553
                     /locus_tag="WJK66_001783"
     CDS             3356..3553
                     /locus_tag="WJK66_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579452.1"
                     /translation="MPSENRSAGVCGALYAVLQDKAQGRAAGCIVATVFLPDGVKNTV
                     ATPTDNEKREQCRLNRVLRRA"
     gene            complement(4597..4672)
                     /locus_tag="WJK66_001784"
     tRNA            complement(4597..4672)
                     /locus_tag="WJK66_001784"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4636..4638),aa:Glu,seq:ttc)
BASE COUNT         1062 a         1173 c         1335 g         1199 t
ORIGIN      
        1 ggtttttgtt aatccactat agtttaattt gtctgtccgc attgccgcta attgtaccac
       61 ttcatcgtat ttccttttcg gttgaaaccc cgccctttag ggcggcagga tcagacttta
      121 tttgggaggg ggtgtaaccc cttccaaatc aggacgacac atagggaggt gctttatgtg
      181 tcgtcctgtg tgttgaaata tatcggacgc gtttgaacag ttgtgcggca aactgtttta
      241 gcggcaggca agagatgccg tctgatgccg tctgaagccc attgtccgtc attcccacac
      301 aggcagttgc tacggttgct gaagcccctt agccgtcgcg caggcggttg ctacggttac
      361 taaaatcccg tcattcccgc gcaggcggga atccagtccg ttcggtttca gttgtttctg
      421 ataaatcttg cagcgttggg tttttagatt cccgctttcg cgggaatgac ggtagagcgg
      481 tttcagttgc tcccgataaa tgccgcaatc tcaaatcccg tcattccctc aaaaacagaa
      541 aaacaaaatc agaaacctaa aatcccgtca ttcccgcagc tcttcgtcat tcccgtgaaa
      601 gcgggaatct agacctttta aattttcaca cggcttttga atattgccgt tacccgaagg
      661 tctggattcc cgcctgcacg ggaatgacgg cagagcggtt tcagttgttt ctaaataaat
      721 ttttgcagcg ttggattttt agattcccgc tttcgcggga atgacggggc ggtcggatgc
      781 cgcctgaagc ccgacaaagc ccccaatgcc gtctgaaacc tcgtcattcc tctgctgtcc
      841 gcaacttttc gtcattcccg caaaagcggg aatctagagc ctctaaattt tcagacggct
      901 tttgaatatt gccgttgccc gacggtttgg attcccgcct gcgcgggaat gacggtggag
      961 gacgatgccg tctaaagccc attagcggtt tcagacggca tttttgccgg agcggcagtc
     1021 aggaaaactg cgctgcgcca cggtttgcca aatcgtcggc gcgttcgttt tccgcgtgtc
     1081 ccgcgtgtcc tttaacccaa gtccagctga cttggtgctg cccaaccaga acgtcgagtt
     1141 ctttccacaa gtcgtcgttt ttgacgggct gtttggcggc ggttttccag ccgttgcgct
     1201 tccaaccgtg tatccagttt tccatgccgt ttttgacgta ttgcgagtcg gtgcagatga
     1261 tgacggtgca gcggcgtttg agcgatttca gtccttcgat gacggcggtc agctccatgc
     1321 ggttgttggt ggtttgcgct tcgccgccga aaagttcttt ttcgcggctg ccgtagcgca
     1381 tcaacacgcc ccagccgccc gcgccgggat tgcctttgca cgcgccgtcg gtgtaaaggt
     1441 aaacggtttt gtccattatt gtgcctttgt gtggtgtccg gcgtgattat aagcggtttg
     1501 tgcgccgtgc cgtctgaaag attgcggcgg attaaaatag gcatataatg tgcaacttta
     1561 tttttgaata tgaaggagtg gatagtgggt caaagcggcg agttgttttg tttcggacag
     1621 atgcccgtat ggaaggcgga aaacctgccg gaagttttgt tgtcgggcta ttcgtccgaa
     1681 ggaggggaat gggtctgcct gaatgtgttg cagggcgatg tcgaagtccg cacgcaggac
     1741 ggggcggcgg aggtttggtc ggcagaaagc ggcgattgcg tgtttgcgcc gcagcaggtg
     1801 ttttcggtca aaccgaaaac ggacgatgcc gaaatccgtt tgtcgatgta ttgtgtggcc
     1861 gaagattatt tccacaaaaa atacggcatg agtgccacgc attctgcagt ggcggcggct
     1921 caggatatcg tacctgcggg cagggcgctc gatatgggct gcgggcaggg gcgcaacgcg
     1981 ctgtttctcg gtttgaaggg gtttgaagtt actgcagtcg atcacaatcc cgccgccttg
     2041 gcaaacgtgg cggagctggc agaggcggag ggtttgaacg tccgcacgct ggaatatgat
     2101 ttgaacgccg ccgccttgca gggcgagttt gattatattg tggcaacagt ggtgctgatg
     2161 ttcctgatgc cgcagcgcgt acccgacgtg attgccgata tgcaggcgca tacggcggcg
     2221 ggcgggtaca acttgattgt atcggcaatg gatacggcgg atttcccctg cccgatgccg
     2281 ttccccttca aatttaaaga gggagaactg aaggattatt atcgggattg ggagctggtc
     2341 gaatataaag aagaattggg cgcgatgcac gccaaagacg aaaacggcaa tccgatacgg
     2401 tttaaatttg taaccatgct ggcgaaaagg cctgaataat cttcaaaaat gccgtctgaa
     2461 cattttgttt cagacggcat ttttacggtt agtcccgata ggcccaatga ttgaccgggc
     2521 cgtgtcccgc gccgatttcc aaagggcttg agattgccgc cgtgatgtag gacttggcag
     2581 tccgtacggc tttgcaaacg tctaagcctt ttgccaactc ggcggtaatg caggcagaaa
     2641 acgtgcagcc cgtgccgtgc gtgtgggcgg tcggaaagcg cgggctgtcc aattccagcg
     2701 tttcgttttg cgtaaacagc caatccgtgc agcgtccgct gctgctgcca ttcaaatgtc
     2761 cgcctttgat gacgacattt ttgacaccgt aatcaagcag gatttttgcc gcacgttccg
     2821 catctttacg gttttcgata tgcacgccgg tcagggcttc cgcctcgggc aggttggggg
     2881 tcaatacatc cgtatcggga agcagcaggc gcgtcagtgc cgcaacggcg gaatcctgca
     2941 acagcggcgc accgcctttg gcaatcatca cagggtcgag tacgcgcctg ccgaagtcgc
     3001 agtgtttcag cttgtcggca acgcattcga tgatttccgc cgtgccgagc attcctattt
     3061 tgtaggcgcg gatgtcaaag tcttccctga ttgcttggat ttgtgcggtg atggtttcgg
     3121 tcgggacgag atgaaccgcc gacacgccca aggtattttg cgcggtaacg gcggtgatga
     3181 cgcacgttcc gaacacgccg cgcatctgaa acgttttcaa atccgcctga atgcccgcac
     3241 cgccgcccga atccgaaccg gcgatggtaa gcgtttgcac aaaagagctt ttcatttgtt
     3301 cctccttaat attgtgtccg ataaaaaggg ctaatgcctg taaaaaagca cggcgatgcc
     3361 gtctgaaaac cgcagcgcgg gggtttgtgg tgcattatat gccgtattgc aggacaaggc
     3421 acagggcagg gcggcggggt gcatcgtagc aactgtattt ttgcccgacg gggtgaaaaa
     3481 tacagttgct acacccactg ataatgaaaa aagagagcaa tgccgtctga accgcgtgtt
     3541 gcggcgagca taaaccggca cgctttattc gtcatttcca caaaaacaga aacctaaaat
     3601 tccgtcattc ctacgaaagc gggaatccag tgtgttcggt ttcagttgtt tttgggtttc
     3661 gggtgatttc caaatcatca ttcccgcgca ggcgggaatc tagcccttgg ggcagcggca
     3721 atatttataa agccgtctga aaattcagaa gttctagatt cccgttttca cgggaatgac
     3781 gaagggttgt gggaatgacg aagtttcaga cggcatcgcc ccgccccgtc attcccgcat
     3841 aagagggaat ccaaaccatt gggcagagca gccttaaagg ttataaaaga ctcgtcattc
     3901 ccacgaaagt gggaatccag acctgtcggc acggaaactt atcggataaa acggtttctt
     3961 caaccctacg ctctagattc ccgcttttgc gggaatgacg aagtttcaga cggcatcaaa
     4021 tgctttgtca ggcttcagac agcatcgtcc accctctgcc gtccttcccg cgcaggcggg
     4081 aatccaatgt gttcggtttc ggttgttttt gggtttcggg taatttccaa atcgtcattc
     4141 ccacgaaagt gggaatccag accattgggc ggcggaaata ttcaaaagtc gtctgaaaat
     4201 tcagaggttc tagattcccg ttttcacggg aatgacgaag ggttgtggga atgacgaagt
     4261 tttagacggc attgaatact ttgatgcttt ggtgggctga agcccaccct acaatataga
     4321 gaaaatgccg ccaaacggta tggaagcgga aacaatggtg gggctggctt cagaaacaat
     4381 ggtagggtgg gcttcagccc accgctttag acggcatccg cgcaccgtca ttcccgcgca
     4441 ggcgggaatc cagccttgtc ggcacggaaa cttatcggat aaaacggttt cttcaaccct
     4501 acgctctaga ttcccgtttt cacgggaatg acgaagagtg tgagaatgac ggttcataaa
     4561 taaaaaaacc tgcttaaaac aagcaggttt ttgaattggc acgcccacgg ggattcgaac
     4621 cccggttgcc gccgtgaaag ggcagtgtcc taggcctcta gacgatgggc gcgtgggaat
     4681 gggcattata catacttcta gaaatttagc aatacgatgc cgtctgaaac ttcgtcattc
     4741 ccgcgaaagc gggaatctag aatctccaa
//
LOCUS       DAWKOG010000152         4707 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.152, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000152 DAWKOG010000000
VERSION     DAWKOG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.152"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            112..1503
                     /locus_tag="WJK66_001785"
     CDS             112..1503
                     /locus_tag="WJK66_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEZ7579453.1"
                     /translation="MSGQLGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLVFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATAMVSDQPIEGEEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAVILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
     gene            complement(1565..2317)
                     /gene="rlmB"
                     /locus_tag="WJK66_001786"
     CDS             complement(1565..2317)
                     /gene="rlmB"
                     /locus_tag="WJK66_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEZ7579454.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYIQEGKSDARTREVLEK
                     AANENIRVYFVDADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMSGESDLYHCNLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKV"
     gene            complement(2384..2872)
                     /locus_tag="WJK66_001787"
     CDS             complement(2384..2872)
                     /locus_tag="WJK66_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEZ7579455.1"
                     /translation="MTVLTVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYAGGY
                     QIYGAGVLWTVGLISLAGILADYVAGIWGTKYTGAGKLAVRGALAGSIIGIFFSLPGL
                     ILGPFIGAAAGELIERRNMLQAGKAGLGTLLGLVVGTAFKIGCAVSILFILLVKYIAY
                     LF"
     gene            complement(2877..3596)
                     /locus_tag="WJK66_001788"
     CDS             complement(2877..3596)
                     /locus_tag="WJK66_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family DNA transformation protein"
                     /protein_id="HEZ7579456.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLNGTLPQEITQHDKTRLFAELKNHPPVAAFPPQEEMEHFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIIASAHNTCIADCNVSRHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            complement(3600..4541)
                     /gene="miaA"
                     /locus_tag="WJK66_001789"
     CDS             complement(3600..4541)
                     /gene="miaA"
                     /locus_tag="WJK66_001789"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEZ7579457.1"
                     /translation="MPTPKAFALLGPTAGGKTALALKIAETLPVEIISLDSALVYRDM
                     DIGTAKPSASERAFVPHHLIDIITPVQTYSAARFVEDCTRLIGEITARGKYPLIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQQVDPETACRLKPNDSQ
                     RIGRALEVYYLTGKPMSEHLGRQSPHTLPFDLHTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPGLTADSPAIRCVGYRQAWEYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSCTRLIEAAKRFFGA"
BASE COUNT          872 a         1131 c         1514 g         1190 t
ORIGIN      
        1 tggtaaacgt gtgggccaga cggatgccgt ctgaaatgca aattgaagcc gtgcggcaga
       61 ttcgctacaa tccgcgcttg gatttttcaa cctttaaaat aaggaaatac aatgagcggt
      121 cagttgggca aaggtgcgga tgcgcctgat ttggtgtacg gtttggagga taggccgccg
      181 ttcggtaatg cgctcttgag cgcggttacc catcttttgg cgatttttgt gccgatgatt
      241 acgcccgcgc tgattgtggg cggcgcgctg gaattgccgg tggagatgac ggcgtatctc
      301 gtgtcgatgg cgatggttgc gtcgggtgtc ggcacttatt tgcaggtcaa ccgcttcggg
      361 ccggtcggtt cggggatgct gtccatccag tcggtgaatt tctcgttcgt tactgtcatg
      421 attgcgctcg gcgcggggat gaaagagggc ggtttgacta aggatgcgat gatttcgacg
      481 ctcttgggcg tatcgtttgt cggcgcgttt ttggtgtgtt tctcggcgtg gcttctgccg
      541 tatttgaaaa aggtgattac gccgacggtc agcggcgtgg tcgtgatgct cattggtttg
      601 agtttggtac acgtcggcat taccgatttc ggcggcggct tcggcgcgaa ggcggacggc
      661 acgttcggct cgatggaaaa cttggggctg gcatcgctgg tgttgctgat tgtgttggtg
      721 ttcaactgca tgaaaaaccc gctgttgcgc atgagcggca ttgcggtcgg gctgattgcc
      781 ggctatatcg tcgcgctgtt tttgggcaag gtggattttt ccgcgctgca aaacctgccg
      841 ctggttacgc tgcccgtacc gtttaaatat ggttttgctt ttgactggca cgcgtttatt
      901 gtggcgggcg cgattttctt gttgagcgtg tttgaggcgg tcggcgattt gaccgcaacg
      961 gcaatggtgt ccgatcagcc gattgaaggc gaggaataca ccaaacgcct gcgcggcggc
     1021 gtgttggctg acggcttggt gtcggtgatt gcgacggctt tgggttcgct gccgctgacg
     1081 acgtttgcgc aaaacaacgg cgtgattcag atgaccggcg tggcttcgcg tcatgtgggc
     1141 aaatatattg ccgtgatttt ggtgctgttg ggtctgttcc ccgttgtcgg tcgcgcgttt
     1201 acgacgattc cgagtccggt gttgggcggc gcgatggttt tgatgttcgg cttaattgcg
     1261 attgcgggcg tgcggatttt ggtcagtcac ggcatccgca ggcgcgaagc ggtgattgcg
     1321 gcaacgtcgg tcggtttggg cttgggtgtc gcgtttgagc cggaagtgtt taaaaacctg
     1381 cccgtcttgt tccaaaactc tatttccgcc ggcggcatta cggcagtctt gctgaatttg
     1441 gtcttgcccg aagataaaac cgaggcggcg gtcaagtttg ataccgacca cttggaacac
     1501 tgattttgaa aatgaatgcc gtctgaaaca gaatccctgt ttcagacggc attgtttttg
     1561 aggcttatac tttttcgttt tttaatacgc gttgtcggcg tgtttcactt aataccattc
     1621 cggcagacac ggagacgttc atgctttcga ctgtgccgaa catgggtatg gacaccagca
     1681 tgtcgcaatg ttcgcgcgtg aggcggcgca taccgtcgcc ttcgttgccc attacccacg
     1741 ccgcgctgtc gggcagattg caatggtaaa ggtcggactc gccgctcata tcggtgccga
     1801 taatccaaat gccgtattct ttcaattcgc gcagggtgcg ggcgaggttg gttacggtga
     1861 tataggggac ggtttccgcc gcaccgcagg cgactttgct gacggtggcg ttcagccccg
     1921 cgcttttgtc tttcggtgcg atgacggcgt gtacgcccat tgcgtcggcg gtacgcaggc
     1981 acgcgccgag gttgtgcgga tcggtgatgc cgtcgagtat cagcagcagc ggcggttcgc
     2041 tgaggttttc caatacgtct tcgaggtgga cgtggttttt ggaggcatcg ataaatccga
     2101 ccacgccctg atggcgcgcg cctttgctga tggcgttgag gcggtcggca tcgacaaaat
     2161 atacgcggat gttttcgttt gccgcctttt ccaacacttc gcgcgtgcgt gcgtctgatt
     2221 tgccttcttg gatgtagagt tcgacgatgg atttggggtt ttgccacaat cgggcgttga
     2281 cggcgtggaa gccgtagatg ggtctttggt ttgccatgat ggtgctttgt aaaaagggtt
     2341 cagacagcat tatagcaatt tgccggtatg ccgtctgaaa gggttaaaac aggtaggcga
     2401 tgtatttcac caacaggata aacaagatgg atacggcgca gccgattttg aacgccgtgc
     2461 cgacgacaag ccccaacagc gtacccaagc ccgctttacc tgcctgaagc atattgcgcc
     2521 gttcgatcag ttcgcctgcc gccgcgccga taaagggacc gagtattagt ccgggaaggg
     2581 agaaaaatat gccgatgatg ctgccggcca atgcgccgcg aacggcgagc ttgcccgctc
     2641 cggtatattt tgtcccccat atgcctgcca catagtccgc cagtatgccg gcaaggctga
     2701 tgagtccgac cgtccacaaa acgcccgcgc cgtagatttg gtagccgccg gcataggcaa
     2761 gcagccatgt tccggcaaac atcaatgcca atccgggcag ggcggggtaa acgatgcccg
     2821 ccgtgccgac ggctatcagg gcgagggcga ggatgacggt cagtacggtc ataggttcaa
     2881 ccttttcttt tgttttgaaa aaaacggctt aacacggcgc ggcattcttc ttgcaggatt
     2941 ccgccccgta tggcggtgtg cgtattgagg cgtttgtcgg caaacaggtt gacgatgctg
     3001 cctgccgcgc cggttttggg ttctgccgcc ccgtagatca cgcgcctgat tcgtgcctgt
     3061 atcagtgcgg acgcgcacat ggcgcagggt tcgagggtga tatatatgtc gcatccgtca
     3121 aggcggtagt tttgcatttc gctgcctgcc tgtgccaagg cgttgatttc ggcgtgccgg
     3181 ctgacattgc agtcggcaat gcaggtgttg tgtgccgatg cgatgatttt gccgtctgaa
     3241 acgatgactg ccccgacggg tatttcgccg tcagcggcgg attgttctgc ttggcgcagt
     3301 gcttcgcgca tgaagtgttc catttcttcc tgcggcggaa aggcggcgac gggcggatgg
     3361 tttttcaatt cggcaaaaag gcgcgttttg tcgtgttgcg tgatttcttg cgggagtgtg
     3421 ccgttcagca gcgactcaag ctgccacagc gtgcttttcg tgagggtcaa acccgatgct
     3481 ttgagcagca gaaaggcttt gaccgaaccg ttttgccgca gttcttcgag cgtacggata
     3541 ccgagcctgt gcagggcggc gacggttttg ggggcgagcg gcggtgtggt cagcatggtt
     3601 tatgcgccga aaaaccgttt tgccgcctca atcaggcgtg tgcatgaagt gccgtctgaa
     3661 aacgggtcgg caacgcagtc taaaggtgtt ttgcgcaacc aagtcagttg gcgtttggca
     3721 agttggcggg tggcggcaat gcctttctcg acaaatgccg ggaaatcggt ttttccgtcc
     3781 agatattccc atgcctgacg gtagccgacg cagcggatgg cgggggagtc ggcggtcagg
     3841 ccgggatagc ggcggcgcag gttttctact tcgccgataa agccctgttc aagcatcagg
     3901 tggaaacgca gggcgatgtt ttcatgcagg cgggcgcggt tttcgggaat cagggcggcg
     3961 gtatgcaaat cgaaggggag cgtatggggg ctttgtctgc cgaggtgttc gctcatcggt
     4021 ttgccggtca gataataaac ttccaaagcg cgtccgatac gctggctgtc gttcggtttc
     4081 agacggcatg ctgtttcggg atcgacctgt tgcagggtgc ggtagaggaa atccaagccg
     4141 tacatttgtt tttgttcgtc caagtcggca cgcaggcagg catcggcttc gggcaaatcg
     4201 ttcaaacctt gggtcagggc gcggaaatac atcatcgtgc cgccgacaat cagcggatat
     4261 ttcccacgcg ccgtgatttc gccaatcaga cgcgtgcagt cttcgacaaa acgggcggca
     4321 ctgtatgtct gaacaggcgt aatgatgtcg ataaggtggt gcgggacaaa ggcgcgttcg
     4381 gaggcggacg gtttcgccgt gccgatgtcc atatcgcggt aaaccagcgc ggaatcgagg
     4441 ctgatgattt cgacaggcag ggtttcggca attttgaggg cgagcgcggt tttgcctccg
     4501 gcggtcggcc cgagcagggc aaaggctttc ggggtcggca taacgtttca ggtttggaaa
     4561 aatacggatt atagcggaaa gcgtgccgac gttatatttt ggtttgcgga agcacgccga
     4621 cggcaagggg gcgtgtttac cgtatgcctt tatatagtgg attaacaaaa accagtacgg
     4681 tgttgcctcg ccttagctca aagagaa
//
LOCUS       DAWKOG010000153         4699 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.153, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000153 DAWKOG010000000
VERSION     DAWKOG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.153"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(416..952)
                     /locus_tag="WJK66_001790"
     CDS             complement(416..952)
                     /locus_tag="WJK66_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEZ7579458.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDDEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYVAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(986..1342)
                     /gene="folB"
                     /locus_tag="WJK66_001791"
     CDS             complement(986..1342)
                     /gene="folB"
                     /locus_tag="WJK66_001791"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEZ7579459.1"
                     /translation="MDKIFLHGIKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            1398..2000
                     /gene="plsY"
                     /locus_tag="WJK66_001792"
     CDS             1398..2000
                     /gene="plsY"
                     /locus_tag="WJK66_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEZ7579460.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALVWLVMAFGFKVSSLAALTATIAAPLAALFF
                     MPHTSWIFATLAIAILVLLRHKSNIVKLLEGRESKIGGSR"
     gene            2077..2922
                     /locus_tag="WJK66_001793"
     CDS             2077..2922
                     /locus_tag="WJK66_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEZ7579461.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSEAQKVGEYRQGIALLRQAKNLPTELNGFIAHIQKILPSPELYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     TVLIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
     gene            3164..4138
                     /locus_tag="WJK66_001794"
     CDS             3164..4138
                     /locus_tag="WJK66_001794"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEZ7579462.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDSFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKEKPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPSKPGKLRLMYEANPMA
                     LILEQAGASASNARQDILGIRPESLHQRVAVIMGSSEEVDYLNRLHSK"
     gene            complement(4297..4515)
                     /locus_tag="WJK66_001795"
     CDS             complement(4297..4515)
                     /locus_tag="WJK66_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEZ7579463.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
BASE COUNT         1205 a         1389 c          997 g         1108 t
ORIGIN      
        1 ctcaaattca aattgggctt ttaggtctat aacaaagaaa aagccttggt aggataaacc
       61 taccaaggct ttttcttaaa aaaacgctct attaccgtgc ctaaaaatgc aaaaaagtaa
      121 ccgtcataac ccttttgcca aaatcaatat catcatccga tgaatgaatt gacggtatat
      181 aagaaagaat taataagctc ataaaccggt aagaaaaatc agtaacaaag atattcatta
      241 ttaaccattt taaaaattat catgttcgtt tgggtaatta ttataccctt ttccctctta
      301 ttactaaagc acgaatgact ttaaaggacg cgcccagaga caagccgaaa aggcatatct
      361 catccgccaa acaaaatgcc caccgactta ctccggcagg cagttcaaca ccctgtcaat
      421 ccttcatcag ccagtattgc aaaccgatta atgtcttgcc gtctttaatt tcatcgtttg
      481 ctaatgcctg acggacttct tctttcgaca tcaatacggt ttccgtaatc tcgtcttcgt
      541 cattggcaag cgtactgccc aaacgcacgc cttccgcttc gaacagatac attttttcgt
      601 tgcaaaaacc gaccgccgta taaaaactgt aaagcaggcg cacgctgtcg gcaacataag
      661 gcgtttcttc agccagttct ctcagcgcac acgctgccat atcctcgccc gccacatcca
      721 gcttgcccgc aggaagttcc aatgtcgcct gatttgccgc ataccgccac tgccgtacca
      781 aaacgacctt ttcatcatcc gttaccgcca acacgcacgc cgctcccgga tgacggatga
      841 cgatacgctg cccttcattg ccgttgggca acctgacctt atccctgctg atgctgacaa
      901 aaccgccctc gtaaatggtt tcgccgccta attttacttc cctcaaatcc acttcatatc
      961 tcctttccgt ttcctattct gccgttcaat cttcacgctt gccgcgctcg atttccacgc
     1021 caacctcgcg cacgccttcc aaaatacccg gcttgacgat tttcacgcgt acccacaccg
     1081 cgccgaaata tcccaaaacc aaatcggcaa tatattccgc caacgcttcc aaaagcagga
     1141 aatcctgttc tttcagatgt cggcgcagtg tttcgcatac ctcggcataa tgtaccgtat
     1201 tggcaatatc gtcgtccgaa cccgctttct cgggaacgcc gatgtccaaa tcgacaatca
     1261 gggtctgcaa ccgttcgcgt tcccagccgt acacgccgat aagcgtatcc gccttgatgc
     1321 cgtgcaaaaa gattttatcc ataatccgcc ccgttttttg ctaaaataac cgcaccattc
     1381 taagtaaagt accacaaatg ttcaacatac cggctgttgc cgtttcctat ctgatcggct
     1441 cactttcttt cgccgtcatc gtttccaagt attacgggat ggacgacccg cgcacctacg
     1501 gatcgggcaa tcctggggcg accaatgttt tacgcagcgg caaaaaaaag gcggccgcgc
     1561 tgacgctctt gggcgatgcc gccaaaggtt tagttgccgt tttgcttgca cgcgtgcttc
     1621 aagaaccgct cggtttatcc gacagcgcaa tcgctgccgt cgcactcgcc gcgctggtcg
     1681 ggcatatgtg gccggtgttt ttcggattta agggcggcaa aggcgtggca acggcattgg
     1741 gcgtgcttct ggcactctct cccgcaactg ccttggtctg cgcgttggtt tggcttgtta
     1801 tggcattcgg cttcaaggtg tcctcccttg ccgcattaac cgccacaatc gccgcccccc
     1861 ttgccgcact gttttttatg ccgcatactt cttggatttt cgcaaccctc gcaatcgcca
     1921 tattggtgtt gctccgccac aaaagtaata tcgtcaagct gctcgaaggc agagaaagca
     1981 aaatcggcgg cagccgctga tttcccatcc atccctgttt atgccgtctg aaccccgctt
     2041 cagacggcat ccgaatatcc cgccgtttta ccgactatgc tcagttacag acacgcattc
     2101 cacgccggca accacgccga tatgctcaaa cacttcacct tgtttttagt gctgcaatat
     2161 ttcaaccgca aagacaagcc ctactggtac atcgacacgc acggcggcgc gggtgtgtac
     2221 aatctcgaag gcagcgaggc acaaaaagtc ggcgaatacc ggcaaggcat cgccctactt
     2281 cgacaagcaa aaaacctgcc aaccgagtta aacggcttta tcgcgcacat acaaaaaatc
     2341 ctgccctcgc ccgaacttta ctgcggttct ccgtggctgg cgcaatcgct gacccgcgtc
     2401 ggcgacaaat tacgcctgtt tgagctgcac cccaccgact ttgtccatct gcaaaacaat
     2461 atgggcgaag caggattggg caaacgcgga caagtgttgc gcgaagacgg ttacaaaggg
     2521 ctgatttccc tgctgccgcc cccgccgcgc cgcgccaccg tcctcatcga cccgccctat
     2581 gaggaaaaac aggactaccg gcgtgtaacg gaaacgctga aagccgcttt aaaacggttt
     2641 gagtccggct gttatctcat ttggtatccc tgcctcagcc gcgaagaaag ccgcaagctg
     2701 cccgaagaat tgaaaaaact cctgcccgac aactaccttc acgccgaact tcacgtccac
     2761 gcgccgaaag ccgacggttt cggtatgcac ggcagcggaa tgttcgtcat caatccgccc
     2821 tatctgttgg cggaacaatt ggcggcaaac ctccccgccc tgacgcggct gctggcgcaa
     2881 gacgaaggcg cgcgctacct tttggacagc aaaatccgct gaccccgatg ccgtctgaaa
     2941 cccttcagac ggcatctccc atcaaacaaa accggacgat ataccggcag ccggcacttt
     3001 taagaataaa tatcccctca tccgcctgcc gcgttccgac ttgccccatg ccgttttttt
     3061 acactacaat cccctagact atcctgcaaa acgcaccaaa ggaatacgcc acaataacct
     3121 gccgcctttc agacggcaca aacctttccg aaacgagcaa actatggaca cactgacccg
     3181 attcctcccc gaacacctgc aacaaaacca gctgcccgaa gcactcggcg gcgttttact
     3241 gtccgttgtt tccgcctgca ccgaaatcaa cgccaaagtc cgcctcggcg cactggccgg
     3301 cgtattgggt atggcgggca cgggcaatat tcagggcgaa gaccaaaaaa aactggatgt
     3361 tatcgccaac aacatcatga ttgacacact caaagccaac cctgccgttg ccggtttggc
     3421 aagcgaggaa gaagactctt tcgtaagtgc cggtgaaaac ggacgctatc tcgtcctatt
     3481 cgatccttta gacggatcgt ccaatattga tgtcaacatt tccgtcggta cgattttctc
     3541 catccttgaa aaacccgaag gcgcattggc aaccgaatca ttccttcaaa cgggcagaca
     3601 gcagcttgcc gctggttacg ttctttacgg gcctcaaacc cagctcgtat tcacattcgg
     3661 acacggcgta tatatgttca cgctcaatgc cgaaaacgaa tttgtgctga caaaagaaaa
     3721 accgaaagta cccgaaagta ccaaagaatt tgccatcaat atgtccaacc gccgccactg
     3781 gctgcccccc gtccaacaat acatcgacga gctcttggcg ggcgaaaccg gcacgcgcgg
     3841 caaaaactac aatatgcgct gggtagccag catggttgcc gaaatccacc gcatactgat
     3901 gcgcgggggc gtgttcatgt atccgcaaga caaacgcgac ccgtccaaac ccggcaaact
     3961 gcgcctgatg tatgaagcca atcctatggc cctgatactc gaacaagccg gcgcatcggc
     4021 aagcaacgcc cgtcaggaca tactcggcat ccgtcccgaa agcctgcacc agcgcgtcgc
     4081 cgtcattatg ggcagcagcg aagaagtgga ttacctcaac cgcctgcatt caaaataaaa
     4141 ggcagcctga ccgataaaga aaggcggcag cgcgcatccg acgctgccgc cgatatttcg
     4201 tttatagcct taaaaagata gaaaaccacc tttccgaacg gcaaaaatgc cgtctgaaaa
     4261 gttttcagac ggcattttaa aaattaaagc ccgaatttat ttgcccgctg ctttttgcag
     4321 gatggcgtac aactcgtctt tcagtttcaa tttggctttt ttcagctcat cgatggtttc
     4381 cgcaccgctg gtaaccggat tgttgaccag accggtaatt ttatcgtcca gctcgttgtg
     4441 ttcgtcgaac agacgggcga agcgagaatt ttcctgtttc aatttggaaa tcaaatcacg
     4501 gtattctggg aacatatcgc tttcctctct tggtttacgg ttaaaaagtc aaattgaatc
     4561 tttacattaa ttataacaaa gataatattt ttatccagtg ttttatcgga tacggcaaaa
     4621 ttttctttta acaccgccgt ccggttgata aaaacgttga caatatcacg ctttacgcat
     4681 aaattttcag cctttccta
//
LOCUS       DAWKOG010000154         4656 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.154, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000154 DAWKOG010000000
VERSION     DAWKOG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.154"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..981
                     /gene="mafB"
                     /locus_tag="WJK66_001796"
     CDS             <1..981
                     /gene="mafB"
                     /locus_tag="WJK66_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ7579464.1"
                     /translation="RSIKLNPTDTRSIRQRISDNYNNLGSNFSDRADEANRKMFEHNA
                     KLDRWGNSMEFINGVAAGALNPFISAGEALGIGDILYGTRYAIDKAAMRNIAPLPAEG
                     KFAVIGGLGSVAGFEKNTREAVDRWIQENPNAAETVEAVFNVAAAAKVAKLAKAAKPG
                     KAAVSGDFSAAYNTRTTRKVTTETEGLNRIRQNQKNSNIHEKNYGRDNPNHINVLSGN
                     SIQHILYGDEAGGGHLFPGKPGKTTFPQHWSASKITHEISDIVTSPKTQWYAQTGTGG
                     KYIAKGRPARWVSYETRDGIRIRTVYEPATGKVVTAFPDRTSNPKYNPVK"
     gene            996..1226
                     /locus_tag="WJK66_001797"
     CDS             996..1226
                     /locus_tag="WJK66_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ7579465.1"
                     /translation="MKTLDERIKNLGKSLEDRIDANLIDAALEYITFSERLLAFETLC
                     DYIEDFNIQLTEKESQEISFINKEFGIESTSD"
     gene            1375..2082
                     /locus_tag="WJK66_001798"
     CDS             1375..2082
                     /locus_tag="WJK66_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ7579466.1"
                     /translation="MVKTADGYKAIARIRVGDRVFAKDETSGKTGYKPVTARYGNPYQ
                     ETVYIEISDGIGNSQTLISNRIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWHTYFVKGNQAETEGVWVHNDCPYGEKYRTEVGSYTNTHESGRT
                     YSGKGTRQRSQISGAREARVNNDPHIATDFTPAKNNREAFKDESRRIDAHGGSKSTNN
                     YNRIESPGKKYRKQDGD"
     gene            2085..2408
                     /locus_tag="WJK66_001799"
     CDS             2085..2408
                     /locus_tag="WJK66_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HEZ7579467.1"
                     /translation="MIVDEKSTSDVLKQYQELGFDLTKPMIIEFFIGGSQKNLQSIES
                     RLISYRQDFQISIEQDEFGEMWTCYCSVNIVPSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
     gene            2467..2694
                     /locus_tag="WJK66_001800"
     CDS             2467..2694
                     /locus_tag="WJK66_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579468.1"
                     /translation="MEIKFNTLGVILNGVNPEEKFIKIIDDQENTGGFLILLSSNDKF
                     SSFDSYDDWVENLEILKEYLQESHWIIKWVG"
     gene            2821..3363
                     /locus_tag="WJK66_001801"
     CDS             2821..3363
                     /locus_tag="WJK66_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEZ7579469.1"
                     /translation="MINHIEKYTGTISHGSKSDSGSQYKLNIAAIPSSPNRDLKTYIT
                     LGLSKYNLNYKSRFEILFVCSLKYDENQIFPFLRWLAETIIENKKILLRGQVIYLPRS
                     IVDSTKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFEQ
                     FLLDNEVDNLSDMNRKPFVW"
     gene            3498..4175
                     /locus_tag="WJK66_001802"
     CDS             3498..4175
                     /locus_tag="WJK66_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hint domain-/BECR-fold containing toxin"
                     /protein_id="HEZ7579470.1"
                     /translation="MVKTADGYKAIAHIQTGEYVFAKDEASGKTGYKPVTARYGNPYQ
                     ETVYIKVSDGIGNSQTLVSNKIHPFYSQGKWIQADRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVANWHTYFVKSSQAETEGVWVHNDCSSRKTPSTPIYEDDSEAYAAAK
                     KLGYRKIKERTRNDAAIFKKGKSYISRDVDSHNGGAWKEASSPKNLNRKETRNGTFDK
                     NLNRIGD"
     gene            4190..4588
                     /locus_tag="WJK66_001803"
     CDS             4190..4588
                     /locus_tag="WJK66_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ7579471.1"
                     /translation="MCELKNFRRNITCFEGYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRRKYPYPMDIPRDIVIGIGTIIDFLMVQNWKLFEIKASPWLPKSVKINERYERFR
                     VMLRYIFTDLDVEDWKFFYFPIQHSKGRLR"
BASE COUNT         1576 a          892 c          981 g         1207 t
ORIGIN      
        1 cgcagtatca aactcaatcc gaccgacacc cgcagcatcc ggcaacgcat atccgacaac
       61 tacaacaacc tcggcagcaa tttctccgac cgcgccgatg aagccaacag aaaaatgttc
      121 gagcacaatg ccaagctcga ccgctggggc aacagcatgg agtttatcaa cggcgtcgcc
      181 gccggcgcgc tcaacccctt tatcagcgcg ggcgaagcct tgggcatagg cgacatactg
      241 tacggaacgc gctatgccat agacaaagcc gcaatgcgca acatcgcccc cttgcccgcc
      301 gagggcaaat tcgccgtcat cggcggcttg ggcagcgtgg cgggctttga aaagaatacg
      361 cgcgaagccg ttgaccggtg gatacaggaa aaccccaatg ccgccgaaac cgtcgaagcc
      421 gtcttcaacg ttgccgccgc cgccaaagtc gcgaagttgg caaaggcggc aaaaccgggg
      481 aaggctgcgg ttagcgggga tttttctgct gcatacaata caagaacaac tagaaaagtt
      541 actacagaaa cagaggggtt aaatagaatc agacagaacc agaaaaatag taatatacat
      601 gagaaaaatt atggaagaga taatcctaat catattaatg ttttatctgg aaattctata
      661 caacatatac tgtatggaga tgaagcagga ggtgggcatc tttttcctgg caaacctggt
      721 aagacaacat tcccccaaca ttggtcagcc agtaaaataa ctcatgaaat tagtgatatc
      781 gttacatccc caaaaacgca atggtatgca cagactggaa caggcggcaa atatattgct
      841 aaaggaagac cagccaggtg ggtatcatat gaaacgagag atggaattcg tatcagaaca
      901 gtttatgaac ctgcaacagg aaaagtggta actgcattcc ccgatagaac ctctaatccc
      961 aaatataacc ctgtaaaata atgagtattt gaaagatgaa aacattagac gaacgaataa
     1021 aaaatttagg aaaatctctt gaagatagaa ttgacgctaa tctaattgat gccgcactag
     1081 agtacattac tttttcagaa cgtttgctag cttttgaaac gctatgtgat tatatagaag
     1141 atttcaatat ccagcttacg gaaaaagaat cccaagaaat ctcttttatt aataaggaat
     1201 ttggaattga aagtacatca gattaataaa tattaggtcg gtttaaaaac ccaacaatca
     1261 aaaagtcgtc tgaaactgtg tttaatttac agacgacctt tccttcattt gaagcaggat
     1321 attgagaact gagttcttca aaaatcctac acctgctcct tccacggcag caccttggtc
     1381 aaaacggcag acggctacaa agccattgcc cgtatccgag tcggcgaccg cgtcttcgcc
     1441 aaagatgaaa ccagtggaaa aactggatac aaacctgtta ccgcccgata cggcaatccg
     1501 taccaggaaa ccgtttacat tgaaatttca gacggcatcg gtaacagcca aaccctgatt
     1561 tccaaccgca tccatccgtt ttacagtcaa ggaaaatgga tacaggcagg tcgtctgaaa
     1621 aaaggcgaca ccctgctttc cgaaagcggt gcaaaacaga cagttcaaaa cattaccctc
     1681 aaacagcagc cgctcaaagc ctacaacctg accgttgccg actggcatac ttatttcgtc
     1741 aagggtaatc aggcggaaac ggaaggggtt tgggttcata atgattgtcc gtatggagaa
     1801 aaatatagga cagaagttgg ttcttatact aatactcatg aaagtggtcg aacttattct
     1861 ggaaagggca caagacaacg ttcccaaatc tctggagcaa gagaagctag ggttaataat
     1921 gaccctcata tagctacaga ttttactcct gccaaaaata atcgagaggc attcaaagat
     1981 gaatctagaa gaattgacgc tcacggtggt tccaaaagta ccaataatta caatcgaatt
     2041 gaaagcccgg ggaaaaaata ccgaaaacaa gatggagatt aaaaatgatt gttgacgaaa
     2101 aatctacttc agatgtactg aaacaatatc aagaactagg gtttgatttg acaaaaccta
     2161 tgataataga gttttttata ggaggttctc aaaagaatct gcaatcaata gaaagccgac
     2221 tcatttctta tcggcaagat tttcaaatca gtattgaaca agatgaattc ggagaaatgt
     2281 ggacttgcta ttgttccgtc aatatcgtcc cttcccttga aaacatttta gctattgaaa
     2341 ctacgttatt tggtattgca caaaaaaatg attgtaatta tgaaggtttt ggttcgtatg
     2401 ggaactaaca aaaagtgtag gttgggttga aaaaaccata ttcccattaa agattagggg
     2461 atgtctatgg aaataaaatt caatactttg ggggttatcc tgaatggggt caatcctgaa
     2521 gaaaaattca taaaaattat tgatgaccag gaaaataccg gtggatttct gatactgtta
     2581 tcttcaaatg acaaattctc atcatttgac agttacgatg actgggtcga aaacttagaa
     2641 atattaaaag aatacctgca agaatctcat tggataataa agtgggtcgg atagtcggca
     2701 aacgtagtcc gtctgaaacg cttttacttt tcagacggac tttctatcaa tggggacaag
     2761 gcgttttgtc tcaatctggt ctatgattat tcgatacagt gatgggaagc taagaaaaat
     2821 atgattaatc atattgaaaa atatacaggc actatttctc atggctcgaa aagtgattcg
     2881 ggaagtcagt acaaattaaa tatcgcagct attccctctt cccctaacag ggatttaaaa
     2941 acttatatta ctttagggtt aagcaaatat aatctgaatt ataaaagcag gtttgaaata
     3001 ctttttgtat gttctttaaa atatgatgaa aatcagattt ttccattttt aagatggttg
     3061 gcagaaacca tcattgagaa caaaaaaatt cttcttcgag gacaggttat ttatttgcct
     3121 agaagcattg ttgattcaac aaaaatggat gcactgtatg tttccgcccc attttatttc
     3181 gatgatgatt ttcaggtctg ttatggcgaa cactacaata tcgttttccc tttgcttgtc
     3241 cccttgtata aacaggaagc cgaattggtg gaaaagaaag gttggaatgc ttttgagcag
     3301 ttcttgctgg ataatgaagt tgacaacctt tcggatatga ataggaaacc gtttgtttgg
     3361 taagggtttt cttaaatttg attaaaggtc gtctgaaatt aagcttgttt ttcagacggt
     3421 ctttccttca tttgaaatag gatattgaga actaagttct tcaaaaatcc tacacctgct
     3481 ccttccacgg cagcaccttg gtcaaaacgg cagacggcta caaagccatc gcacatatcc
     3541 aaactgggga gtacgtcttt gccaaggacg aagcaagcgg aaaaacggga tacaaacccg
     3601 ttaccgcccg atacggcaat ccgtaccaag aaaccgttta cattaaagtt tcagacggca
     3661 tcggcaacag ccaaaccctt gtttccaata aaatccaccc gttttacagt caaggaaaat
     3721 ggatacaggc agatcgtctg aaaaaaggcg acaccctgct ttccgaaagc ggcgcaaaac
     3781 agacggttca aaacattacc ctcaaacagc agccgctcaa agcctacaac ctgaccgtcg
     3841 ccaactggca tacctacttc gtcaagagca gtcaggcgga aacggaaggg gtttgggttc
     3901 ataatgattg ctcgtcaaga aaaacaccat ctacccccat atatgaggat gattcagaag
     3961 catatgcagc agcaaagaaa ttgggatata ggaaaataaa agaaagaacg agaaatgatg
     4021 ctgctatttt taaaaaagga aagagttata tatcacgcga tgtagattca cataatggag
     4081 gggcttggaa agaagcatct tcacccaaaa atctgaacag aaaagaaaca agaaacggta
     4141 catttgataa aaacctaaat cgtattggag attaattatt atgtgtatta tgtgtgagtt
     4201 aaaaaacttc agaagaaata ttacttgttt tgagggatat gacgaaaatt catttattgg
     4261 caaatggtat gatgacgggg tgtgggatga tgaagaatat tggaagctgg agaatgattt
     4321 aatcgaggtt aggagaaaat atccttatcc gatggatata ccaagggaca tcgtgattgg
     4381 aatcggtacc attattgatt ttttaatggt tcaaaattgg aaactttttg aaattaaagc
     4441 ttctccttgg ttgcctaaaa gtgtcaaaat taatgagcgt tatgaaaggt tcagagtaat
     4501 gctccgttat atttttactg atctagatgt agaggactgg aaattttttt actttccaat
     4561 acaacatagt aaaggtagat tgagatgaaa agtttaatta tattggagat acattattat
     4621 gtgtgagttc aaggatatta taagaaacgt tcctta
//
LOCUS       DAWKOG010000155         4608 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.155, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000155 DAWKOG010000000
VERSION     DAWKOG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.155"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(1036..1806)
                     /gene="xth"
                     /locus_tag="WJK66_001804"
     CDS             complement(1036..1806)
                     /gene="xth"
                     /locus_tag="WJK66_001804"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ7579472.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRNAPEDVHIGLPALPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRYGKLVLLGDFNIAPADADCYDPE
                     KWHEKIHCSSVERQWFKNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMAEALKDVRVDLETRALERPSDHAPVTAEFDW"
     gene            complement(1866..2147)
                     /locus_tag="WJK66_001805"
     CDS             complement(1866..2147)
                     /locus_tag="WJK66_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ7579473.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIAELAKSLSLPAT
                     AVSGHLNRLRAGGLVDFTRYHRIIEYRLISEDAAAILRTIRDLENKRAV"
     gene            2249..2482
                     /locus_tag="WJK66_001806"
     CDS             2249..2482
                     /locus_tag="WJK66_001806"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579474.1"
                     /translation="MDDCTGLYVPFPTFFANTRKGGLSDGIKLPPLPCLTLLPNGIIR
                     IVQKMPYNRRTSCPADASVPSGKQNTCLKRCFF"
     gene            complement(2615..3496)
                     /gene="nadC"
                     /locus_tag="WJK66_001807"
     CDS             complement(2615..3496)
                     /gene="nadC"
                     /locus_tag="WJK66_001807"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEZ7579475.1"
                     /translation="MPSENTLFSLPDTLLRPIVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLACLAFQTMDPSVRFQAEIQDGQAVRAGQTLAVVEGNACAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCGSIAQAVRQAKQAVGALTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFLA"
     gene            complement(3572..4510)
                     /locus_tag="WJK66_001808"
     CDS             complement(3572..4510)
                     /locus_tag="WJK66_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579476.1"
                     /translation="MDAYPEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQMRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIVEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
BASE COUNT         1070 a         1154 c         1229 g         1155 t
ORIGIN      
        1 cgacttgcgt ttcatgaaac tgatgcagat atagtggatt aacaaaaatc aggacaaggc
       61 gacgaagccg cagacagtac aaatagtacg gcaacgctgt actggtttaa atttaatcca
      121 ctatattttg taatagctgc cattatgcct actaggatat ataccttcta tataatcctt
      181 atttaaataa aaacccttat aaaaatctgt gttatctgtc gggattgaaa aaaagatttt
      241 atctttctca tcaggtttat atttgctatc aatatcaaaa agagaataag gattacaatg
      301 agttcgacat actaggtaaa aatttatatc ttggatcatg aaatattcga tatttatcag
      361 gatcgaaaga tgctaaaaat tcatcaatat cggtactgga tattttgtca tcactaataa
      421 acatcgctag agatgggcga ctttgatttt ccatttgttg attagtaaag gatataaata
      481 ctaacttgcc attgtcaaac cacatagatt tatatagtgg attaaattta aaccagtacg
      541 tcgttgcctc gccttgccgt actatttgta ctgtctgcgg cttcgtcgcc ttgtcctgat
      601 ttttgttaat ccactatagt tattgccttt gggagaatca attctgacag cacataacat
      661 aggttcatct ttacagtcgt tatattgggt ttcatcaaac tcaactgcat agcacaataa
      721 aggcaatagg aaaaaaagga gattaattta ttactataca taaggcagtc tgactattca
      781 ttctaaaata aatccggttt ttaatatttg attctgctcc ggattatata cataataaat
      841 tttactgctc caaaatctga tattaaccct atggatttca taaatttttc agaaaaatag
      901 gctgtgcttg aacaatccgc cgactccggc tgtttgtcag ggaaaaggca attattttcg
      961 gttagtcagc agcctatcct attgaatcac ttaaagatat ggatgtttgt caacgccata
     1021 tcaaaacacg gtcttttacc aatcgaattc tgccgtcacc ggcgcgtggt cgctcggacg
     1081 ctccagcgcg cgcgtctcca aatcgacacg gacatccttc aacgcctccg ccatcgcagg
     1141 cgacaccaaa atatggtcga tacgcaggcc cagtttgcgt tggaacatcg cgccgcgata
     1201 gtcgaaccag gtatagaacg cgccttcggg atggacttgg cgcaggctgt cggtcagtcc
     1261 caaatccagc aggtttttga accactgccg ttcgacggac gaacagtgga ttttttcgtg
     1321 ccatttttca gggtcgtaac agtccgcatc ggcaggcgcg atattgaaat cgcccagtag
     1381 caccagtttg ccgtagcggg tcatttcatc gcggacaaat tcggttaacg cggcaaacca
     1441 ttgttcttta tatttgaatt tcgggctgtc gagggcttcg ccgttgacgc aatagacatt
     1501 gatgacgcgc acaccgccga cggttgccgc aatcacgcgc cgttgcggat cgtccggcag
     1561 cgcgggcagc ccgatatgca cgtcttccgg cgcgttgcgg ctgacgattg ccacgccgtt
     1621 gtaggttttc tgcccgctcc aaacacagtg ccagcccatc atttgcaaag cggcggccgg
     1681 aaatttgtcc tgatcgagtt tgagttcctg caaaaccaaa atatcaggcg gattgtcggc
     1741 aagcaggttt tgcacctgcg gcagccgcac attgagcgaa ttgacgttcc aagtggtgat
     1801 tttcataaca tttccgaaaa atgccgtctg aaacgttcag acggcaaaag gaaaggattt
     1861 taacactata ccgcgcgttt gttttccaaa tcgcggattg tccgcagaat cgccgccgcg
     1921 tcttcggaaa tcaggcggta ttcgataatg cggtggtaac gcgtaaagtc caccagaccg
     1981 cccgccctca ggcggttcaa atggccggaa acggcggtgg ccggcaggga taaggatttt
     2041 gccagttcgg cgatattgcg ttcgctgtcc aacagttgga tcagtatcgc catacgttcg
     2101 ggatgcgcca taagtttgag tatggtgtgg agcggtattg tgttcatcgg tattcctaaa
     2161 acctttgccg cccgccaagc ggattgacgg gcgacagata tggatgattg tgattatctt
     2221 tataaatcgt attataaatc aaatatttat ggatgattgt acaggattgt atgttccttt
     2281 tcccactttt tttgcaaaca cgcgtaaagg cgggctttca gacggcataa aactgccgcc
     2341 cctaccctgc ctcaccctgt tgccgaacgg aatcatccgc attgttcaaa agatgcctta
     2401 caaccgccgc acgtcttgcc ctgccgatgc ctccgtgcct tcgggcaaac aaaatacctg
     2461 cctgaaacgc tgttttttct aaagcatcac gaccttcacg aaaaatgccg tctgaagaat
     2521 atttcagacg gcatttcctc cttgaggcaa taaccggatt acggttaggc ggttccttgt
     2581 ctgacatatc agacaactgc cgacacccta aaactcatgc caagaaatcc aaacctatgt
     2641 ccaacgagcg gctgctgtgg gtcagatagc cgagggcgat gccgtccacg ccggtttgcg
     2701 ccacgcgctt caggcggtcg aagccgatgc cgcccgatgc ttcgcaatag acggtgtggg
     2761 ggtgggcggt ttgcgtgtgg cagcggtttg ccgcttcttt cagggtttcg tcgtccatgt
     2821 tgtccagcaa aatccgttcc gcgcccgctg cgatggcttc gtccagttgt gccaacgtgt
     2881 ccacttcgat ttccacgcag gtcaatgctc cgaccgcctg ttttgcctgc cgaacggctt
     2941 gggcgatgct gccgcaatag gcgaggtggt tgtctttgat gagcacggcg tcgtccaagc
     3001 ccatgcggtg gttcacgccg ccgcctgccc tgacggcgta tttttgcagg acgcgcagca
     3061 gggggatggt tttgcggctg cacacgatgt ctgtaccgta ttcggcgact tcggcaacgg
     3121 cacgcgcggt ggcggtggcg atgccgctta agtgcgtgag gtagttgagt gcggtgcgtt
     3181 cggcggcgag cagcgcgcag gcgttgcctt cgacgacggc cagcgtctga cctgcgcgga
     3241 cggcttgtcc gtcttggatt tcggcttgga aacggacgga cggatccatc gtctgaaagg
     3301 cgagacacgc caagtccata ccggcgataa caccgtcttc gcggctgacg aggaagagtt
     3361 tggcggtttt gtcgggggcg ataacggcgg cggacgtaat gtcgccgcgc ctgcccaagt
     3421 cttcgctcaa ggcttgttcg actatggggc gcaacagggt gtcgggcagg gaaaagaggg
     3481 tgttttcaga cggcatgagg tttcctttgt cgaaaaagat tgatgttgca ccgatggggc
     3541 aaaacttcat ttgatgccgt ctgaaaacgg gctaacggct gcccagcggc agtccgatgt
     3601 ccgaaatcag cctgtcgggc aggacgtcgt cgcggaagcg gcaaagctgc gcgggacggc
     3661 ctttgctgcc cgatacgccg gtatccgacg gctcgatgag gttttgctgc tgaatctggc
     3721 ggcggaagtt ttgcttgtgc agcaatcttc cgctgatggc ttcgacactg ttttgcagtt
     3781 gcagcagcgt gaattcgggc ggcatcagct caaaaatcac ggggcggtat ttgattttgg
     3841 cgcgcaggcg ggacagggcg gtcgccagta cgcgacggtg atcgtggcgc atgggctgcc
     3901 ccgtgagcgc gaagtcgaag tttgcctgcg gctcggcggc ttccactatc agaccgcttt
     3961 catacagcat ttcatagcgt tgtaaaacgt attcttccga ccagttttcc ggttcgaccc
     4021 cccagcacaa atgaatgcgc ttgagccgca tttgacgcac ttcctccgtg tccgccgaat
     4081 ttgcccaaat gcgcaggcgg ctgacgacgg cgtcgcgctg cccgccgtcg gtgcgcaagt
     4141 cttcccacgg gaaatagccg tagcagtcct gccatttcgc atccgggtgc aggatgctgt
     4201 cggctgcctc gcgcaccagc cccaaatagc tgacgtacaa gacgggcatg ccgtgttcgt
     4261 tgcggcggtg ggtatcgaca aaggtgtaaa gctgttccac atagcccata ggctgcgaag
     4321 tctgcttggc aacccacagc ttcacgcccg cctgcaggga attgcgcaga ggggagagcg
     4381 ggccgttggg caggagtgtg ccttgggcga cggttaatac ccgcaggccg ccgtcggtaa
     4441 cggcaatcaa tacgggaacc agctcgacga tgctttgcgg cggggcttcg gcttcggggt
     4501 aggcatccat gctcggcttt ctgcgggtgg gaaaggcttt attgtatagt ggattaacaa
     4561 aaatcaggac aaggcgacga agccgcagac agtacagata gtacggaa
//
LOCUS       DAWKOG010000156         4575 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.156, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000156 DAWKOG010000000
VERSION     DAWKOG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.156"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(58..1851)
                     /locus_tag="WJK66_001809"
     CDS             complement(58..1851)
                     /locus_tag="WJK66_001809"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEZ7579477.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAESQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIVKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWATNRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGSMNAESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGATLQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            2097..3206
                     /gene="proB"
                     /locus_tag="WJK66_001810"
     CDS             2097..3206
                     /gene="proB"
                     /locus_tag="WJK66_001810"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEZ7579478.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIATESGVPVYICSSLKPDALAEAADNQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     GVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKLRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            3219..4481
                     /locus_tag="WJK66_001811"
     CDS             3219..4481
                     /locus_tag="WJK66_001811"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEZ7579479.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALFAMADSLEAAAADI
                     LAANRQDLEAAAGKIPESMTDRLLLDGKRICAMADGIRAVAALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KAGLEQTRIDPDALQLIEDTGREGSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRDRIEAGLPPVRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVW
                     HIETHSTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
BASE COUNT         1050 a         1243 c         1270 g         1012 t
ORIGIN      
        1 gccgcaatgc cgtctgaagc ctttttatgc ttcagacggc atcgtctgtt ttcacgctca
       61 aatcgtaccg ctgccttgcg ctttcggttt ggcggcacta aattccagct tgctcaaagc
      121 ggaaaggtag gctttggcgg tggcgaccaa aacgtcggta tccgcgccct gaccgttgac
      181 gacgcggttg ccgcgcgcca gacggacgct ggtttcgccc tggctttccg taccttgcgt
      241 gacggcattg acggaataaa tctgcaaagt cgcgccgctt tgcgccacgc tttcaatcgc
      301 tttgaaaatt gcatcaaccg gaccggaacc ggttgcggaa gcgcgttttt cttcgccttt
      361 gatgctgaac acgatgtcgg cgcgcggctc ttcgccggtt tcggtgctga ttttttggga
      421 aatgaatttg tagctctcgg cattcatgct gcccatttcg tcggatacca gtgcgtgcag
      481 gtcttcatcg aagatttcgc gttttttgtc ggcgagttct ttgaagcggg caaacgcggc
      541 gttcagtgcc tcttcgcttt ccaactcgat gcccaaatcc gccagcttgg ttttgaaggc
      601 gttgcggccg gacaatttgc ccaagctcaa acggtttgtt gcccagccga ccgattcggc
      661 ggacataatc tcgtaagttt cgcggtgttt cagcaccccg tcctgatgga tgcccgattc
      721 atgcgaaaag gcattggcac cgacaatggc tttgttgggc tgcacgggat agcccgtaat
      781 ggtggacacc agtttggacg aaggcacgat ttgcgtggta tcgatgccgg tttccaagcc
      841 gaacaagtcg tggcgcactt tcaacgccat cacgatttct tcaaccgaag cattgcctgc
      901 acgttcgccc aagccgttga cagtacattc cacctgacgc gcaccgcctt ttaatgcggc
      961 aagcgaattg gcaaccgcca agcccaaatc gttgtggcag tgtgccgacc aaacgacttt
     1021 gccgccgttg ggcgttttga caatcagttc gcggaaaaat tcttcggttt tgtacgggat
     1081 ggaatagccg acggtatcgg gaatattgat ggtggtcgcg cccgcttcaa ttaccgcgcc
     1141 gcagatttcg gcaaggaaat cgatttccga acgcaacgcg tcttcgcagg aaaattccac
     1201 atcgtcggtg tattcacgag cgattttcac cgctttgacc gccgcctcaa tcacctgctt
     1261 cggcttcatc ttcaatttgt actccatatg gatggggctg gtggcgatga aggtgtggat
     1321 gcgttttttc ggcgcgggcg caacggcctc acccgcctga cggatgtccc gctcgatggc
     1381 acgggacaat gaacagaccg ttgatttggt aatggttttc gcaatcgcat tgaccgcctc
     1441 gaaatcgccc gggctggcag cggcaaaacc cgcttcgatg atgtccacac ccaatttttc
     1501 cagctggcgg gcgacgcgga ttttttcctc tttggtcata gcggcgccgg gcgattgttc
     1561 gccgtcgcgc agggtggtgt cgaaaatgat aacgcggttg gcctgtgtca tttctgttct
     1621 ctccagagat tcgttttttt gtaaaaaagc attcaaatcc agcggcctgc cttgcgattc
     1681 ggcaagcgcg gtcagctttt gcagttgcgc caaaggcagc gacccgcgcg tgcgccattt
     1741 atagatggcg gcggtcgtcg cggcattttc gggatcgtgc tgtttcaacg cttcggcaag
     1801 cgcgttcacg ccgccgaaat aagcaaccaa gcggtctata tctaattgca tggtagtcct
     1861 tgtctaaaat aaatcagcct acagcagaaa tatggacagg attatacgca ttttggacaa
     1921 aacggcaata taaatttgaa aaaatctcaa taaaccgcca tagattttat taattttata
     1981 cattttgtcc attttattgc agacgcggcg tgattgttga caattgcggc agaacagggc
     2041 aaattcggcg gcacaaggtg gcaacgagac aggacaaacc aacaaggacg gaaacaatga
     2101 aatacaaaag aatcgtattt aaagtcggca catcttcgat tacccattcg gacggcagtc
     2161 tctcgcgcgg caaaatccaa accatcaccc gccagcttgc cgcattgcat catgcgggac
     2221 acgagctggt cttggtgtct tccggcgcgg ttgcggcagg gtttggtgcg ctgggtttca
     2281 aaaaacgtcc ggtcaaaatc gccgacaaac aggcttccgc cgccgtcggg caggggctgc
     2341 tgatggaaga atatacggca aacctgtctt cagacggcat cgtgtccgcg caaatcctgc
     2401 tcagccgcgc cgactttgcc gacaaacgcc gctaccaaaa tgccggcggc gcactttccg
     2461 tgctgctgca acgccgcgcc gtccccatca tcaatgaaaa cgatacggtt tcggttgagg
     2521 aattgaaaat cggcgacaac gacacattga gtgcgcaagt ggcggcgatg atacaggcag
     2581 acctcttggt gctgctgacc gacatagacg gtctttacac gggcaacccg aacagcaatc
     2641 ccgatgccgt acggctggac aaaatcgaac acatcaacca tgaaatcatc gaaatggcgg
     2701 gcggatcggg ttcggcaaac ggcacgggcg gtatgctgac taaaatcaaa gcggcgacga
     2761 ttgcgaccga gtccggcgta ccggtctata tctgttcctc gctcaaaccc gatgcacttg
     2821 ccgaagcggc agataatcag gcggacggct cgtttttcgt cccccgtgcc aaaggtttgc
     2881 ggacgcagaa gcaatggctg gcgttctatt ccgaaagcag gggcggcgtt tatgtggacg
     2941 aaggtgcgga acacgctttg tccgaacagg gaaaaagcct gctgatgtcg ggcattgccg
     3001 gaatcgaagg gcatttttcc cgtatggaca ccgtaaccgt gtacagcaag gcaaccaaac
     3061 agcctttggg caaagggcgc gtcctgttcg gctctgccgc cgccgaagac ctgctcaaat
     3121 tgcgtaaggc gaaaggcgtg ttcatccatc gggacgactg gatttccatc acgcccgaaa
     3181 tacgcctgct tctgaccgaa ttttagaaaa gaaaacccat gtcaaacaca caaaaacagc
     3241 ttgcccttgc caaagcggca aaaaaatccg tcaacacggc ggatacggaa gaaaaaaacc
     3301 gcgcgctgtt tgctatggcg gacagcttgg aagcggcggc ggcagatatt ttggcggcaa
     3361 accgtcaaga cttggaagcc gcggcaggca aaattcccga aagcatgact gaccgccttt
     3421 tgttggacgg caaacgcatt tgcgcgatgg cggacggcat cagggcggtt gccgcactgc
     3481 ccgaccccgt gggcgaaata ctggaaacct cgactctgcc caacggcttg gaaatcgtca
     3541 aaaagcgtgt ggcgatgggc gttatcggca ttatttacga aagccgcccg aacgttacgt
     3601 ccgatgcggc ggctttggca ctgaaaagcg gcagcgcggt cgtactccgc agcggcaaag
     3661 atgcattcca atccgcacgc gccatcgttg ccgcactcaa agccggtttg gagcaaaccc
     3721 gcatcgaccc cgacgcgctc cagttgattg aagacacagg gcgtgagggc agttacgaaa
     3781 tgatgagggc gaaagattat ctagacctgc tgattccgcg cggcggggcg gggctgatac
     3841 gggcggtggt tgaaaatgcc gtcgtgccgg tcattgaaac gggaacgggc attgtccaca
     3901 tttatatcga caaggacgcg gattgggaca aggcgctccg tatcgtgtac aacgccaaaa
     3961 ccagccgtcc gtccgtgtgc aactcgatgg aagtgctgct ggtacatgaa gacattgctg
     4021 ccgacttcct gcccaagctc gaacggctgt tggttcgcga ccgcatagaa gccggactgc
     4081 cgcccgtccg cttccgtttg gatccgcagg cggcgcggca tatcggcggc gaagcggcgg
     4141 gtgcagacga tttcgatacc gagtttttag actacatcct cgctgtgaaa accgtcgctt
     4201 cggtcgaaga ggcggtctgg cacatcgaaa cccacagcac acaccattca gacggcatcg
     4261 ttaccgaaaa ccgccacgct gcggactatt tcacgactca tatcgattct gccgccgtgt
     4321 atgtcaacgc gtccacgcgc tttaccgacg gcggcgaatt cggcttgggt tgcgaaatgg
     4381 gcatctccac gcaaaaactc cacgcccggg gcccgatggg tttgaaagag ctgacgagtt
     4441 acaaatacat cgtacaaggc acgggacagg tcagggaata atccgaattc aaatgtctga
     4501 tatagtggat taactttaaa ccggtacggc gttgcctcgc cttagctcaa agagaacgat
     4561 tctctaaggt gctga
//
LOCUS       DAWKOG010000157         4565 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.157, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000157 DAWKOG010000000
VERSION     DAWKOG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.157"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(272..379)
                     /locus_tag="WJK66_001812"
                     /pseudo
     CDS             complement(272..379)
                     /locus_tag="WJK66_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(425..1153)
                     /gene="pdxJ"
                     /locus_tag="WJK66_001813"
     CDS             complement(425..1153)
                     /gene="pdxJ"
                     /locus_tag="WJK66_001813"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEZ7579480.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAGFTKILTDAGIRVSLFIDADDRQIQAAYDVGAPVVELHTGAYADAR
                     SHAEQLKQFERLQNGAHFAGDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(1177..1968)
                     /gene="recO"
                     /locus_tag="WJK66_001814"
     CDS             complement(1177..1968)
                     /gene="recO"
                     /locus_tag="WJK66_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEZ7579481.1"
                     /translation="MSEHRVNHEPVFLLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPVSASWYGSQELKTLHRAEWGGGWRQPQGRALFSGLYVNELVLK
                     LTAREDPMPELYDALAKVMEAVCREANHIADLRRFEWKLLNALGVAPDLHADGTGGDI
                     LADKTYRLMPEEAVMPVCEDADALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRKETARETVPTSDGTASNAV"
     gene            complement(2002..3129)
                     /gene="pheA"
                     /locus_tag="WJK66_001815"
     CDS             complement(2002..3129)
                     /gene="pheA"
                     /locus_tag="WJK66_001815"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEZ7579482.1"
                     /translation="MLECTINYCSGEIMSQTIDELLIPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDDCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEIVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(3166..4398)
                     /locus_tag="WJK66_001816"
     CDS             complement(3166..4398)
                     /locus_tag="WJK66_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEZ7579483.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLAVYCLAVAAIVFASSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQYFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRITAWRL
                     KTGAHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHDGSATVMAATMTASTSCGIAL
                     LWLCSHRAWKENEQSEYL"
BASE COUNT         1009 a         1309 c         1260 g          987 t
ORIGIN      
        1 cgccttgtcc tgatttttgt taatccacta tattacataa tcatcgcctc aatatttaat
       61 atcccaaaat atagatttaa aaccatcgcc tttgtggttt gtcagcattc ctaattgaga
      121 tagaaagtca aataatttct tgtttctccg gctgccatca agatggttta gattaagacc
      181 gcgaacaata caataaccaa tacgctgtac tgtattgtga atttcttcta tcaaactatt
      241 attgtttgca gataaaatat acttttgatt catatagtga attaaattta aaccagtacg
      301 gcgttggctt gccttgccgt actatttgta ctgtctgcgg cttcgccgcc ttgtcctgat
      361 ttaaatttaa tccactataa catatccctg tcgtgaaatg ctgtctgaaa gggtttgcct
      421 gcccttacgg cagcagcctt gccctgaaca tcgcctcctt catctggcgc acggcttcgg
      481 gcagtccgag gaagagggct tgggcaatca gcgaatgccc gatgttcagt tcgcggatgg
      541 cgaggatttg ggcgatgggg gtaacgttgt gtatggtcag tccgtgtccg gcgttgacga
      601 ccaagcccaa atcgccggca aaatgcgcgc cgttttgcag acgctcgaat tgcttgagtt
      661 gttcggcgtg gctgcgcgcg tcggcatacg cgccggtgtg cagctcgaca acgggcgcgc
      721 cgacatcata ggcggcttgg atttgcctgt cgtcggcatc gataaagaga gagactcgga
      781 tgcctgcgtc ggtcaggatt ttggtgaacc cggcgatttt ttcctgttgc gccaatacgt
      841 ccaagccgcc ttcggtcgtg atttcctgac gtttttcagg cacgatgcac acgtcttccg
      901 gcatcacttt aagcgcgttt tcgagcattt cttccgtcaa cgccatttca aggttcaggc
      961 gcgtgcggat ggcgtttttg acggcaaaca catccgcgtc tttgatgtgg cggcggtctt
     1021 cgcgcaggtg catggtaatc aaatccgcac cgtgcgtttc ggcaaccagt gccgcctcca
     1081 cggggctggg ataagtcgtg ccgcgcgcat tgcggacggt agcgatgtgg tcgatgttga
     1141 cacctaaaag cataatcttt ccttttattt ctgccttcag acggcatttg aagccgtgcc
     1201 gtccgaagtc gggacggttt cccgggcggt ttctttgcgg tcaaactgcc gtatctgttc
     1261 caacacctgc cgcgatttca gcccctcggg caacaggtgg cggataaaaa gccgtgtgat
     1321 tttcaatgcc tgttgcaggc tttcggcggt gcggaaactg ccttcgcgca aatcgatcag
     1381 gctttgccct tcgacaattg cgccggcttc gtgcgaaagc gcgtctgcgt cctcgcatac
     1441 gggcatgacc gcctcttcgg gcatcagccg gtaggttttg tccgccaaaa tatcgccgcc
     1501 cgtgccgtcg gcgtgcaaat cgggggcaac gcccaaggca ttgagcagct tccactcgaa
     1561 gcggcgcaaa tcggcaatat ggttggcttc acggcacacc gcctccatca cttttgccaa
     1621 cgcgtcgtac agttcgggca tcggatcttc gcgcgcggtc agtttcaaca ccagttcgtt
     1681 cacatacaga ccgctgaaca acgccctgcc ctgcggctgc cgccatccgc cgccccattc
     1741 ggcacggtga agggttttga gttcctgact gccgtaccac gacgcactga cgggcacgaa
     1801 aggaaccagc acgccgcgca attcgctctg ccgtttgcgc gcgcttcttg ccaaaagtgc
     1861 cacacgcccg taacggcggc tgaacgcttc gacccgcagg ctgctttcgc gccagggcga
     1921 ggaagccaac aggaagacgg gttcgtggtt gactcggtgt tcggacatca tgcctgaaat
     1981 gccgtctgaa cgctgccgcc gctacaaaac ggcggtcggg tacgaaccga tgactttgac
     2041 gaacgaagcg cgttcgccca agcgttccaa tgccgtctga atctgcgcgt cccggcggtg
     2101 tccttcgatg tcgatgaaga acaggtattc ccacaaaacg gatttactcg gacggctctc
     2161 aaacttggtc atggaaatac ccgactcagt cagcggttgc agcagcgagg caaccgcgcc
     2221 tgcccggttg ggcgcggaaa cggccagcga agtcttgtcg ctgccgcttg caccggtttc
     2281 gtgatgtccc atcaccaaga agcgcgtggt gttgttcggt tcgtcttcga tgcactcggc
     2341 aaccatatcg agtccgtaga tttccgccgc cgtgcgtccg gcgatggcgg caaccgtacc
     2401 gtcgtccgat tcggcaacca gccttgcggc ttcggcattg ctggacacgg caatccgttc
     2461 ggcgttgggc aggtgtctgc ccaaccagtc gttgcactgc gccaacgcct gcgcgtggga
     2521 aaagactttg gcaatgcctt cggtgctgcc gttgttttta cgcaaaaggt tgtggtggat
     2581 gcgcaaaacg atttcgccgc acgcctgcaa cgcggtaacg gcaagcaggt ctaacgtgcg
     2641 accgaccgag ccttcggtcg aattttccac gggggcgacc agataatccg cctgacgcgt
     2701 ttcaacctgc ttgaagcagt cgtctatggt cggacacgcc atggtgtgcg cggcatgacc
     2761 gaaatgctta atcgccgcct gttgggtaaa cgtgccctgc ggccccagat aggcgatggt
     2821 cagcgggcgt tctacggcga ggcactcgct catcacttcc cgaaacaggc gtgctaccga
     2881 ttcgtcgggc agcgggcctt tgttcaaatc ctgaatgcgg cgcaacacgg cgacttcgcg
     2941 ttcgggacgg tacactgcgc ccgtgccttt cagttcgccg atggcgtgtg cgtgttgcgc
     3001 gcgttcgttg agcaggcgca ggatttccgc atcaatggtg tcgatggcgt tgcggtgcgg
     3061 gatgaggagt tcgtcgatag tttgggacat aatttcgcct gaacagtaat ttatcgtgca
     3121 ttctaacatc aaatgccgtc tgaagcggtt tcagacggca tcggtttaca agtattcgct
     3181 ctgttcgttt tccttccacg cccggtgcga acacagccac agaagcgcaa tgccgcaaga
     3241 ggtggacgcg gtcatcgttg ccgccatcac ggttgccgaa ccgtcgtgca agaaggtcgc
     3301 cgccataccc acccccgcgc cgattaagga ctggaacacg cccaacacgg cgttcgcgct
     3361 gccgccctct tctttgaaat aagacataaa gcatgcctgc gtgttcgcgc cgaccaagcc
     3421 ctgcgtgccg acggaaaaca tcacgcacgc aaccaagagc cagaacggag gcaacccgaa
     3481 aaacagcacg gcggcgagtt gggacaggtt ggcggcaaat tggacgacaa tcccccacag
     3541 caggatgctt tgcggatgcg cgccggtttt gagccgccac gcggtaatgc ggttgaaaaa
     3601 catcatcgtg atgatgttga gtgcaaacgc ccaagcgtat tgatgaggcg taacacggta
     3661 aagctgctgg tacacgaagg aagattcggt cagaaaggcg aacatcgaac cgaagctgaa
     3721 tgcctgaaaa aacagataac ccatcgcggc acgggttttc aatacgcgtt tgaaccgccc
     3781 agccaccagc ccgaacacat cccgtccgat tttgccgccg acggcaggct tgggcaggaa
     3841 atactgcacc aaaccgagca gcaccagcga atacgccgcc agaaaaacaa aaatcgcctg
     3901 ccagccaccc aagccctgca acagtgcgcc gaccatgggt gcgaccagcg gcacaaccat
     3961 caaaatgatg ccgataaggg caaacatctg ggcggctttg cgtccggaat aataatcgcg
     4021 caccattgcg ccgacgatga ccacagtcat gcccgcaccg aatgcctgca cgacgcgcag
     4081 gttgaggagc tgttcggcac tcgaagcaaa tacgatggcg gcaacggcaa ggcagtagac
     4141 ggccagtccg gtcagcgcga cgggtttgcg ccctttgatg tcggacaccg aaccgccgac
     4201 cacctgtccg aacgccgtgc cgaacataaa caaactcagg ctctgttcga tgcggtggac
     4261 atccgcgttc agcgactgcg ccatttcggg aatcgcgggc aggtaggcat cgatggaaaa
     4321 cggcatcagc gtaaccagca tcgccatcaa aaccgccatc agtttttcgc tcatttcagg
     4381 ataatgggca gaaggcataa attctcgtcc gattcgcaaa caaaccgccc ggcggcggaa
     4441 aacacaatac gcggaaacgg aaaattttaa cacaccggca caaacaggcg gcaacaaaag
     4501 ccccggcaaa accggacgaa ccggaacgat atagtggatt aacaaaaacc agtacagcgt
     4561 tgcct
//
LOCUS       DAWKOG010000158         4505 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.158, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000158 DAWKOG010000000
VERSION     DAWKOG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.158"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            22..256
                     /locus_tag="WJK66_001817"
                     /pseudo
     CDS             22..256
                     /locus_tag="WJK66_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
     gene            424..2199
                     /gene="ggt"
                     /locus_tag="WJK66_001818"
     CDS             424..2199
                     /gene="ggt"
                     /locus_tag="WJK66_001818"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEZ7579484.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLTEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGSYELETGTTALQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            2418..2613
                     /locus_tag="WJK66_001819"
                     /pseudo
     CDS             2418..2613
                     /locus_tag="WJK66_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221594.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2680..3930
                     /locus_tag="WJK66_001820"
     CDS             2680..3930
                     /locus_tag="WJK66_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEZ7579485.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLAKVREQVTALCNKYPVYGA"
     gene            4058..4505
                     /locus_tag="WJK66_001821"
                     /pseudo
     CDS             4058..4505
                     /locus_tag="WJK66_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1240 a         1109 c         1050 g         1106 t
ORIGIN      
        1 aggcacgccc cagtttcccc aatgtggatt ttcagcgcgt gcagtagagg tgctgaccca
       61 attggcagac cctttgcttt tgttaatatc ctagaaaacc cagaaattcg cagtactcta
      121 ccactcatcg ccaatcggcc aacctttcca cagctttggg ttaatggtga gttaatcggt
      181 ggtagtgata ttatcacaaa tgtaccaatc aggtgagcta aaaccactca ttgaagagca
      241 tagcccaaag gtctgattac ttaatatctt aaaataaata tcaaaaaaga ctgctgatga
      301 cattagcggt ctttttttga tggtcaagat tgatagtttg gattgaatta tttagtcata
      361 ttaaatacaa taatcagcat gccttgtatg aatcatcaat caaactcagg cgaaggagtg
      421 cttgtggcta aaacatattt attgactgca ttgataatgt ctatgacaat ctctggatgt
      481 caagtcatcc atgccaatca aggtaaggtt aatactcatt ctgctgtcat cacaggtgca
      541 gacgctcaca cgcctgaaca tgcaacggga ctgaccgaac aaaagcaggt gattgcaagt
      601 gattttatgg tagcgtcagc caatccatta gcaacacaag ctggctatga tatcttaaag
      661 caaggcggta gcgctgcaga tgcgatggtg gcggtgcaga cgacactaag cttggtagag
      721 ccacagtcgt caggcttggg cggtggtgca tttgtgttgt attgggataa taccgccaaa
      781 acattgacca catttgatgg gcgtgagacg gcaccgatgc gtgcgacgcc ggaattattt
      841 ttggataaag atggtcaacc attgaaattt atggaagcgg tggtcggtgg tcgctcggtg
      901 ggtacgcctg ctatccctaa actgatggag acaatacatc agcgatacgg tgtattgcct
      961 tggggaaaat tatttgatac accgatccgc ttggcaaaac aaggctttga ggtgtcgcca
     1021 aggcttgcca tctcggttga gcaaaatcag cagcatttgg cacgctatcc aaaaacagcc
     1081 gcttattttt tgccgaatgg tgtgccgcta caagcaggca gcttgctgaa aaatttagaa
     1141 tttgctgaca gtgttcaggc gttagcagct caaggtgcaa aagctctgca tactggtaaa
     1201 tatgcccaaa atatcgtttc agttgtccaa aatgctaagg ataaccccgg tcaattatcc
     1261 ttacaagatt tatctgatta tcaagtggtg gaacgcccgc ctgtttgtgt gacttatcgt
     1321 atttatgaag tatgcggtat gggtgcacca agctcaggag ggattgctgt gggtcagatt
     1381 ttggggattt taaatgaatt ttcacccaat caggtggggt atgatgctga aggtttacgt
     1441 ctcttgggcg acgcttctag gcttgcgttt gcggatcgtg atgtatattt gggcgaccct
     1501 gattttgtac cagtacccat tcgccagttg atttctaaag actacctaaa acatcgcagc
     1561 cagctgcttg agcagtcgga taaggcattg cctagcgtat ctgcaggcga ttttattcat
     1621 gagtgggtgt cctcacaagc gattgagtta ccttctacca gtcatatatc aattgtggat
     1681 aaggcgggca atgtgctatc aatgacgact tccattgaaa atgcgtttgg ctcaactttg
     1741 atggcaaatg gctacctgct gaataatgag ctgacagact ttagttttga gccaataaag
     1801 cagggcaaac aggtcgccaa tcgtgtcgag cccggcaaga gaccacgctc ctcaatggca
     1861 ccaaccatcg tatttaaagc aggcaagcct tatatggcaa tcggctcccc aggtggtagc
     1921 cgtatcattg gctatgtcgc taagacgatt gtggcacata gtgactggaa tatggatatc
     1981 caagatgcca tcagtgcacc caatttatta aatcgctttg gtagctatga actagaaacg
     2041 ggtacaaccg ccctccagtg gcaacaagca ttaaatgatt tgggctataa gactgacgtg
     2101 cgtgagctaa attcaggtgt tcaagccatt attatcgagc caagcaggct ggttggtggt
     2161 gccgatccac gccgagaagg tcgagtgatg ggtgattaga ggtgaagctt gtattttgtg
     2221 aatttgaccg cattaagtta atattaaaat agggggaaat tgtgagtaat caaacaaaaa
     2281 tacaagccat caagcaagct tctgagcaga tcttcgcaat ttgtgagacg ccaaacacag
     2341 cattacaagc cattcatctg attttaagac atggtggggc aggagagttg tcttggcaag
     2401 tggtgtatca gcgggttatg gctgataaag atgtggttgg tgctggttat ttgattgatt
     2461 ttgcacaaac tgccgaaaat ttaccatttg atgcattgcc tttgggtagc cttgtactaa
     2521 cataaaggta atgagacgct aaaaaccagc atgttaaata aattacctga taatgccaaa
     2581 gaaaatttac gcattatgga ctatctgcct taggttttga agtatttttt tatacaaaaa
     2641 acactcatga taccctgatt acacaaagga gccgagaaca tgttttcaaa aagcgttacc
     2701 ctcgcacaat atgaccccga tttggcagca gccattgccc aagaagacca acgccagcaa
     2761 gaccacgtcg agctgattgc ctctgaaaac tacgtcagct gcgccgtaat ggaagcgcaa
     2821 ggttcccagc tgaccaacaa atacgccgaa ggctaccccg gcaaacgcta ctacggcggc
     2881 tgcgaatacg tcgatattgt cgaacaattg gcgattgacc gcgtcaaaaa actgttcggc
     2941 gcgcaatacg ccaacgtcca accgcactcc ggctcgcaag ccaaccaagc cgtgtacgct
     3001 tccgttttaa aaccaggcga caccattttg ggtatgtctc tggcgcacgg cggccacctg
     3061 acccacggcg cgagcgttaa tatctcaggc aaactctaca acgctgttac ttatggtttg
     3121 gatgaaaacg aagtgctcga ttatgccgaa gtcgaacgcc tcgcgctcga acacaaaccc
     3181 aaaatgattg tggcgggtgc gtctgcctac gcgttgcaaa tcgactgggc aaaattccgc
     3241 gaaatcgccg ataaagtcgg cgcatacctc tttgtcgata tggcgcacta tgccggtctg
     3301 gttgccggcg gcgaatatcc caatcccgtg ccgttctgcg acttcgttac caccaccacc
     3361 cacaaaaccc tgcgcggtcc tcgcggcggc gtaattttgt gccgcgacaa tacccacgaa
     3421 aaagcgttga actcttccat cttcccaagc ctgcaaggtg gcccgctgat gcacgttatc
     3481 gccgccaaag ccgtggcgtt taaagaagca ttgcaacccg agttcaaaca atacgcaaaa
     3541 caagtgaaaa tcaatgcagc cgccatggcg gaagagttgg ttaaacgcgg tttgcgcatc
     3601 gtttccggcc gcaccgaaag ccacgttttc ctcgttgacc tgcaaccgat gaaaattacc
     3661 ggcaaagccg ccgaagctgc tttgggcaaa gcgcacatca ctgtcaacaa aaacgccatc
     3721 ccgaatgatc cggaaaaacc cttcgttacc tccggcatcc gcatcggttc cgccgccatg
     3781 accacacgcg gttttaacga agccgacgcg cgcgtattgg cgaacttggt tgccgacgta
     3841 ttgtctaacc ccgaagacga agccaacctc gccaaagtcc gcgaacaagt aactgctttg
     3901 tgcaataaat accccgttta cggcgcgtaa gcctttttaa aaatattccg ctaagcaatc
     3961 caatgccgcc tgaaatctca taatgtttca ggcggaaacc tttgcaaaat tccccaaaat
     4021 cccctaaatt cccaccaaga catttagggg attttccatg agcaccttct tccggcaaac
     4081 cgcgcaagcc atgattgcca aacatatcaa ccgtttcccg ctattgaagt tggaccaagt
     4141 gattgattgg cagccgatcg aaccatacct gaaccgtcaa agaacccgtt acctccgaga
     4201 ccaccgcggt cgtcccgcct atcccctgct gtccatgttc aaagccgttc tgctcggaca
     4261 atggcacagc ctctccgatc ccgaactcga acacagcctc atcacccgca tcgacttcaa
     4321 cctgttttga cgttttgacg aactgagcat ccccgattac agcaccttat gccgctaccg
     4381 caaccggcga cgcaagacga caccctgtcc gaattgccgg aactgatcaa ccgccaactg
     4441 accgaaaaaa acctaaaaat agagaaagca tccgccgccg tcgttgacgc caccattatt
     4501 cagac
//
LOCUS       DAWKOG010000159         4445 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.159, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000159 DAWKOG010000000
VERSION     DAWKOG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.159"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(63..1205)
                     /locus_tag="WJK66_001822"
     CDS             complement(63..1205)
                     /locus_tag="WJK66_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEZ7579486.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELESLGCTDVQAFDGGVSCRGGL
                     EQVYAANLHSRTAGRILLRLTKGTYRTERDIYKLAKNINWFNWFTLQQTFKVKIEAKR
                     ANVKSLQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLNERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIATGRA
                     PGMMRRFGFEKLQNFDKTLWADLRRRAEAQTRPVRAPIAGSDSDRRIVQTALDNARSA
                     EVDDIVSFGVADAQSVRPNGENGIMVSNPPYGVRLEEVHALQALYPQLGTWLKKHYAG
                     WRVGMFTGDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(1254..1676)
                     /locus_tag="WJK66_001823"
     CDS             complement(1254..1676)
                     /locus_tag="WJK66_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579487.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(1669..2478)
                     /locus_tag="WJK66_001824"
     CDS             complement(1669..2478)
                     /locus_tag="WJK66_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ7579488.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPDQWTGKPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEGSGANVLMEAAQWQDRPEHADSVWMVVQTREQWRRAQEKGADAVVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            complement(2560..2937)
                     /locus_tag="WJK66_001825"
     CDS             complement(2560..2937)
                     /locus_tag="WJK66_001825"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEZ7579489.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVVAEK"
     gene            complement(3137..3904)
                     /locus_tag="WJK66_001826"
     CDS             complement(3137..3904)
                     /locus_tag="WJK66_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ7579490.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFIMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSILGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMVLFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIVKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            complement(3914..>4445)
                     /locus_tag="WJK66_001827"
     CDS             complement(3914..>4445)
                     /locus_tag="WJK66_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEZ7579491.1"
                     /translation="DITFSEDVGECPLHSDSSFSENPESYLVMYVVKSANDGGNSLFL
                     SSSDIVNQLSKTETGKKHLKTLTGNLYPFKTPASFDKKQGVRWGNILSVNTQMIRFRS
                     DCIYKGIEENRNKVSKEMVLALDYLVNVIKNASDIQEFSAQDDGLIIIDNVNGLHART
                     DYTDKNRHYIRARITV"
BASE COUNT         1078 a         1108 c         1101 g         1158 t
ORIGIN      
        1 tcggccggat attaaaaaaa gccgtctgaa catttttcag acggcatttt tgtacacttt
       61 cctcaacggt tcgatccctg caccatatca atcaggaaca ggcggcaatc gaggttgccg
      121 ttataaagcg gaattttgcg ttttggcgac aggcgcatga atttgggcat ttccctgtcg
      181 ccggtaaaca tgccgacccg ccagccggca tagtgttttt tcaaccacgt acccaactgc
      241 ggataaagcg cctgcaaagc gtggacttcc tcaaggcgca cgccgtaggg cggattggac
      301 accataatgc cgttttcgcc gttcggtcgg acggactgcg cgtcggcaac gccgaaggaa
      361 acgatgtcgt ccacttcggc actgcgtgcg ttgtccaatg ccgtctgaac gatgcggcgg
      421 tcgctgtcgc tgcctgcaat cggggcgcgg acggggcggg tttgcgcttc ggcgcggcgg
      481 cgcaaatccg cccacagcgt tttatcgaaa ttttgcagtt tttcaaaacc gaagcggcgc
      541 atcatgcccg gtgcgcggcc tgtggcaatc caagcggctt cgatagcaat cgtgccgctg
      601 ccgcaaaacg ggtcttgaaa cggctgcgtg ccgtcgtagc ctgccgagag cagcagtccg
      661 gcggcaaggt tttcgcgcag cggggcttcg ccggtatcca ggcggtagcc gcgtttgaac
      721 agggcttcgc ccgaagtgtc gataaagatt tcgacattgc gttcgttcaa aaaggcgtgg
      781 atgcggacat cgggcgcggc tttgtccacg ctcggacgtg cgtcgtaaat gtcgcggaaa
      841 gcgtcgcaga cggcatcttt gacggtcagt ccgacaaatt ggaggctttt gacgttggca
      901 cgctttgcct cgattttgac tttgaacgtc tgctgcaagg taaaccagtt gaaccagttg
      961 atgtttttgg caagtttgta aatgtcgcgc tcggttcggt atgtgccttt ggtcaggcgt
     1021 agcaggatgc ggccggcggt acgcgagtgc aggttggcgg cgtaaacctg ttccaatccg
     1081 ccccggcagg aaacgccgcc gtcaaacgct tgaacatcgg tacagccgag gctttcgagt
     1141 tcttgcgata aaacggtttc caagccgcgc gggcaggtgg cgaaaagtgt atataaagta
     1201 ttcatagcgt gtcctttccg gacggatgtt ccggcaatgg cggattgaaa ggtttattcg
     1261 gcggtaaatg ccgtctgaaa tgtccggctg ccgattgtaa tgtccgccgt aaaatcgcgc
     1321 ctgccttcga cacagatggg caggcggatg cgtactgcct gccaagtccc cgacggttgc
     1381 cgctcgaaca tatagcggtt gaaacccata tccatatttt tcatactgaa gctgatgctg
     1441 acctgttccg tgccggcggg cgcgtgttcg atataaatat caaacggttt tttggttgaa
     1501 acggcggcgg cgcggacgcg gcttccgtcc ggcagcgtgc aaccctcggt caaatcgcat
     1561 tgcgccgcca cagcttgcgg ctgctgcgcc tgccaccatt gcaacaaaac gagcttgacg
     1621 gcggcaaagg caatcagcag cagtgcggca agcagtaatt tacggttttt attcatgatt
     1681 cttccctatt gtttcatccc ttaccaccac gtgcgccccc aatccgagcc atagttttcc
     1741 ataacgtgca accgtctgtc cgtttgctgc aagtacgagc ggcacggata cgccctgccg
     1801 cagggcttct gccgcctctt gtgcctgaac atcatcgcac acgcgccaaa cgaccgcatc
     1861 cgcgcccttt tcctgcgccc gccgccattg ttcgcgggtc tgcaccacca tccacacgct
     1921 gtcggcgtgt tcgggtctgt cctgccattg cgccgcctcc atcaaaacgt tcgcaccgct
     1981 tccctctgcc gaacccaaag gcaggacgcg gtacgcgccc atactgtttt ctccgtgcaa
     2041 acccgttttc aggctgccgt acaaacggcg cgggacggac agcgaacgca aaagcgcgcc
     2101 gttggcgggc agcatggggg caacggtaaa atcacccgcc ttctgccaag accattcctg
     2161 cccttcgcgg gattgcggtt tgcccgtcca ttggtcgggg ttgacccata ggaatttcag
     2221 gcagacgcgg gcgtgttcgt aggaatggat tttggtcagc caaggcgtgg cggcgaggat
     2281 gcggatgccg agttcttctt caaactcgcg ttgcaaggct tggaagtcgg tttcgcccgc
     2341 ttcgaccttg ccgccggcaa attcccaata tccggcatag ggtttgcctt cggggcgcga
     2401 gctgagcagg tagttgccgt ctgaatcgag caggatgccg gcaacgacgc ggataagggg
     2461 tcgggtgtct tgaatcatgg cgggtacgcg gttggtcggt cggagtgcaa atgagggacg
     2521 agcaatgccg tcaacgggtt tgccgcattt cagacggcat tatttttcgg caacgacaaa
     2581 cgccaatacg gtgtcttctt cgtcgctcat gctgaggctg acgcggctga tgccttgttc
     2641 ctccagccat ttggacaggg cggggccgta gaaaaattcg ggcttgccca atgcgtcatg
     2701 cccgatgccg atgttgcgga aggaaaccgc gccgcgtatg cccgtgccga cggctttggc
     2761 gaaggcttct ttggcggcaa agcgtttggc gaggtagttg acgggtttgc ccgcttgcgg
     2821 aaattcaagc agctcttccg gagtgaggat gcgcccggca aacgcctgtc cgaatttttt
     2881 gtttaagcgg atgatgcgct tgagggaaac aatgtctgtg ccgatgccgt aaatcatatt
     2941 tgcgctcctt cgcccttggt tgcgggtagt gatgaatgtg atgatgaatg ccgtcaattt
     3001 cttggggcgt taaaatggcg tgaatatcca cttttggggt tttgtcggta acaattttca
     3061 ctgtaacctt ttgcatttga aattctaaaa catcgtgcca attatttaat aaggagttaa
     3121 ggcatttaat aatgaattaa agtttgatta aacccgaatg cccgattttc agagccaata
     3181 ccaataaaac aataaaaatt aacattttaa aaaaatttgg gctaatctta gtccttaacc
     3241 gaattccaac atacaatcca ataacagaca atacggacag taaaaatatt aaaccgtatt
     3301 cactcttatt taataaccaa tactggtctc ttagcatata tatttgaaca attttcgcca
     3361 aaagatagca tagattgctt gattttacga tacgattttt attttctgtt tcgctaagca
     3421 aaaatattaa caatatggga gacatggcat tggttgaacc gccgatgatg cctgccaaaa
     3481 acccaaaaag aaccatattc ttattattgg caactacttg aatatttttt gcttttgcac
     3541 atacatttaa aataccattg acagaataat acaatgtaat gattgccatc agtaaaagca
     3601 gccaagacac tggaagtatc aaaagcaact tcacccccaa aatgctgcca acgacgctgc
     3661 cgatagcaag caatttatag gtttttaaat aataaacaat ctcttgccaa aaaccctttt
     3721 tgttattgct gcatagaacc aacaagctca ttaacaggct tggtaatgcc accaaggcaa
     3781 caaccttaga caatggcatg ataaaagcca atgcggttgt accgagcatc ggaaatccca
     3841 tgcctgtaat tccgtgcagt attgcggcag caacaaaaac gatagattgc attatttctt
     3901 gcataaccgt cctttataca gtaattcttg ctctaatata atgcctgttt ttatccgtat
     3961 aatcagttct ggcatgcaag ccattgacat tgtcaataat aatcaaacca tcatcttgtg
     4021 cagaaaattc ttgaatatca ctcgcatttt ttataacatt tacaagataa tcaagtgcaa
     4081 gtaccatttc ctttgatact ttatttctat tttcttcaat acctttatag atacaatcac
     4141 ttctaaatct aatcatttga gtattgaccg ataagatatt accccatctc acaccttgtt
     4201 ttttatcaaa tgatgctggt gttttaaatg gatataaatt gcccgttaat gtttttaagt
     4261 gttttttacc ggtttctgtt ttagataact gattgacaat atctgatgaa cttaaaaata
     4321 gggaattacc tccatcattg gctgatttta ctacatacat aaccaaataa ctttccgggt
     4381 tttcactaaa agatgaatca ctatgaagtg gacattctcc aacatcttcc gagaaagtta
     4441 tatca
//
LOCUS       DAWKOG010000160         4385 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.160, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000160 DAWKOG010000000
VERSION     DAWKOG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.160"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(45..3077)
                     /locus_tag="WJK66_001828"
     CDS             complement(45..3077)
                     /locus_tag="WJK66_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEZ7579492.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPTASDGYSDSGNGTEEAETEEAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNSAALRETKKRYIDAFEKNETAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQAEAKSPDVSQGQSVSDGTAVRDACRRVSVNLKEPNKATVSAEARISR
                     LIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDEAADIYIEEPAAPDAWVVE
                     PPEVPKVPMPAIDIPPPPPVSEIYNRTYEPPTGFEQAQRSRIAETDHLADDVLNGGWQ
                     EETAAIADDGSEGAAERSNGQYLSETEAFGHDSQAVCPFENVPSERPSCRVSDTEADE
                     GAFPSEETGAVSEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVKVKVV
                     DSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETILGKTCMGLELPN
                     PKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTGSGKS
                     VGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANALNWC
                     VNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDDPEPLEKLPFIVV
                     VVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRI
                     AFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRVVEYL
                     KQFGEPDYVDDILSGGGSEELPGIGRSGDDETDPMYDEAVSVVLKTRKASISGVQRAL
                     RIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            3242..3922
                     /locus_tag="WJK66_001829"
     CDS             3242..3922
                     /locus_tag="WJK66_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEZ7579493.1"
                     /translation="MIFQTVWFSDMVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMVGTSAAFWLAALLMLPYCLLFA
                     GSAGAYPPNALVLILPALAVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            3951..4310
                     /locus_tag="WJK66_001830"
     CDS             3951..4310
                     /locus_tag="WJK66_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEZ7579494.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPAAMSPATGNLFANWTGA
                     LLIGIFAETINHPQWKLLLITGFLGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGAAVK"
BASE COUNT          885 a         1286 c         1125 g         1089 t
ORIGIN      
        1 gccgtctgaa acagtcttca gacggcattt ccatttgcaa aaaatcaagc attgtccaag
       61 gggacgagaa tcgtacggtt gccgttgtgt tccggtgcgg acacaatgcc ttccgcctcc
      121 atctggtcaa tcagacgcgc ggcgcggttg tagccgatac gcaaggcgcg ctgtacgccc
      181 gaaatgctgg ctttgcgcgt tttcaggaca acggatacgg cctcgtcgta catcggatcg
      241 gtttcgtcgt cgccgctgcg cccgatgccg ggcagctctt cgctgccgcc gccgctcaaa
      301 atatcgtcaa catagtccgg ctcgccaaac tgtttcaaat attcgaccac gcggtgcacc
      361 tcttcatccg aggcaaacgc gccgtgaacg cgctgcggat aggcagtacc cggcggcagg
      421 aacagcatat cgccctgacc gagcaggttt tccgcgccca tttggtcgag aatcgtgcgg
      481 ctgtcgattt tgctggacac ttggaacgcg atacgcgtcg ggatgttcgc cttaatcaga
      541 cccgtgatga catcgacgct ggggcgttgt gtggcaagga taagatggat gcctgccgcg
      601 cgggcttttt gggcgaggcg ggcaatcagt tcctcgattt tcttgcctgc cgtcatcatc
      661 aaatcggcaa actcatcgac caccaccacg ataaacggca gtttttccaa aggttcggga
      721 tcgtcgggcg tgaggctgaa cgggttgccg attttctccc cccttgctgc ggcttcggcg
      781 attttttgat tgaaacccgc aagattgcgc acgcccataa agctcatcag gcggtagcgt
      841 ttttccattt cgttaacaca ccagttcagc gcgtttgccg ccagcttcat atcggtaacg
      901 acaggggcga gcaggtgcgg gatgccttcg taaatgctca attccagcat tttcggatcg
      961 atcataatca tacgcacgtc ttccggcgcg gctttgaaaa gcatagacag aatcatcgcg
     1021 ttgacaccca ccgatttgcc cgaaccggtc gtgccggcaa ccagcaaatg cggtgctttg
     1081 cccaagtcgg ttacgacggg ctgtccggtg atgtcctgac cgagcgcgag cgtcagcttg
     1141 gatttggatt cggcaaactc gggcgaattg aagatttcgc tcaggcgtat catttggcgt
     1201 ttcgggttcg gaagttccaa acccatacag gttttgccga ggatggtttc gacaacgcgg
     1261 atggaagcca cgccgagcga acgcgccaag tctttttcca gatttagaac ggaattgccg
     1321 cgcacgccga catcgggttc gatttcataa cgcgtaatca cggggccgga ataagaatcg
     1381 acaaccttga ccttgacttt gaactccgcc aatttttctt cgatggtgat gctgttttcc
     1441 aacagttctt cttcggtttg cgtcgcctcg ggattgaaca gcggcggcag aagcaggtcg
     1501 gttgtcggca ggtgttcgga taccgcaccg gtttcttcag atgggaacgc cccttcatcc
     1561 gcttccgtat ccgatacccg gcaggacggg cgttcagacg gcacattttc aaacggacaa
     1621 accgcctgac tgtcatgccc gaacgcttcg gtttccgaca gatattgccc gtttgaccgc
     1681 tctgccgcac cttcactgcc gtcatctgca atagcggcgg tttcctcctg ccaacctcca
     1741 ttcaaaacat catcggcaag atggtcggtt tcagcaatgc ggctgcgttg cgcctgctcg
     1801 aatcctgtcg gcggctcata ggtacggttg tagatttccg atacgggagg cggcggcgga
     1861 atatcgattg cgggcatggg aactttcggc acttcgggtg gttcgaccac ccaagcatcg
     1921 ggcgcagcag gttcttcaat atagatatcg gcagcttcat cataaaccgg accgccgtat
     1981 cccacagacg aaacggtttc cgtgaaaaca ttttcggttt cagacggcat ttcgacatcc
     2041 cgtttcccga caaccgtccg actttccgga atcaggcgcg aaatccgcgc ctccgcagaa
     2101 accgttgcct tgttcggttc tttcaaattg acggaaacgc ggcggcaggc atcgcggacg
     2161 gctgtgccgt ctgaaacgga ctgtccttgg gaaacatccg gggattttgc ctccgcctgc
     2221 cctgcatgac ggcggaacgg cgcattccgt gcattttcgg ctttgactgc agaaaaggca
     2281 gacggatgct gcttctcaaa atacggctca aatccgtaat ccgcagactc ggaaaacgct
     2341 tctttgtccg catcgaacat acgggaatac gtgcgttgaa gcacagggtc gtccaaaccg
     2401 ataatctgta gcccttccat cggggtatcg gacacgcgga ctttggggac cgctgtttcg
     2461 tttttctcaa atgcatcgat atagcgtttt ttcgtttccc ttaaagccgc gctgtttaaa
     2521 gcacgcgttg cttcttccaa agtgatttcc ttaaaaaccg gacggacggg cgaaatttcg
     2581 ctttcagacg gcatcaaccc ttcagcaata ctccggtcga atgggatgcg gcggttgtcg
     2641 attacggcag ttgcaatgtc ttccgtatcg gcagcctctt cctccgcagc ttctgcttct
     2701 tccgtttccg cttcttccgt cccgtttcca ctgtctgaat atccgtcgga tgcggtcgga
     2761 acatccgcac ggtttgcaga agcaggcgga acgggcctgt ccggttcgga ttcgtgcgcc
     2821 agatagtttc ggcagaaacg gacgacaccg acaaacagcc aatacactgc cgttttgacg
     2881 gcatggaaaa gcatcagggc aagttcggga aaatcgggca taccgtcttt gatttcaggc
     2941 agctccgcct gtttttcccc tttcttttcc tgccacgcag aaacctcgcg catccactcg
     3001 cgttcggatt gtgcgcggac aaaaaacaag ccggcaagcg caagcaacaa aataacgatc
     3061 aaaactatcc aaaacatatg ccgtttcccg caagacaaac gattaaacgg gtttatttta
     3121 acgtgtttcc atcccaaaaa aaacaaccgg tcggcaattc tgccgaaacc gattcggctt
     3181 tcggtttgcc cccgccgaca gtataatagc cgtcctttcc cttaatcttt gcggcattgc
     3241 catgattttc caaacagtct ggttttcaga tatggtactg tcggtttcgt ggattgtcct
     3301 tatcctgata ctggcagctt ccgcgccgtc cgcattccgc tcgcttgccc gctaccgttc
     3361 cgcccttccc ctgtgtaccg taattttttc cgcagcatgg tgtctgaacg cctctgccgg
     3421 cggcggacag cttgcccaga tgaactacca tctgctcgcc gtcaatctgg ttgcactgat
     3481 ggtgggcact tccgccgcct tttggcttgc cgcgctgctg atgctgccct actgcctgct
     3541 gtttgccggt tctgccggcg catatccgcc gaacgctttg gtgctgattc tgcccgcgct
     3601 tgccgtcaac cgcctgtcgc gtatgctggt caaccgcctg ccgcccaata ttttcatctt
     3661 tattttcgtc aacggctttc tcgcttccgc cgccggcatt ctgttgaccg ggctggtgct
     3721 gaccggtatt ttggatgctg caaacgcttt tccgtccgaa atattgtgga cgaccgccct
     3781 gcccgtcttt attctgctgg cgtgggcgga agccttcctc agcggcattt caactgccat
     3841 atttgttgca ctgaagcccc attggatcaa cactttcgac gacaaccgct atctgaagtc
     3901 cgaccgcggc atatggcggt agccttcaga cggcataggc agacaaaacc atgctttcca
     3961 acatcatccc cctttccatc ggtgcggcac tcggtgcaac ggcgcgttgg ctgctcaacc
     4021 tcgccgttcc tgccgccatg tctcccgcca cgggcaacct gtttgccaac tggacgggcg
     4081 cgctgctcat cggcatcttt gccgaaacca tcaatcaccc gcaatggaag ctgctgctga
     4141 ttaccggttt tctcggcagc ctgaccacgc tttccggatt ttcactggaa accgtaaccc
     4201 tgctccaatc gaatcgtcct gcttcagcac ttgccaatat tttcctacac acggcaggtt
     4261 cgctgctgct gacttggctc gggctgaaga taggggcggc agtcaaataa ccgcaacaaa
     4321 ttgcctccct ttgttttgac ataatctata aattagcttc gcttgtttaa atactggctg
     4381 tgcca
//
LOCUS       DAWKOG010000161         4370 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.161, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000161 DAWKOG010000000
VERSION     DAWKOG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.161"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(69..2045)
                     /locus_tag="WJK66_001831"
     CDS             complement(69..2045)
                     /locus_tag="WJK66_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEZ7579495.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVAKNA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2194..3033
                     /locus_tag="WJK66_001832"
     CDS             2194..3033
                     /locus_tag="WJK66_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEZ7579496.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GATVVRDLAALLGKPLVAPAKTQAKALARIDESACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            3232..4263
                     /gene="gap"
                     /locus_tag="WJK66_001833"
     CDS             3232..4263
                     /gene="gap"
                     /locus_tag="WJK66_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ7579497.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKIHLESGAKKVLISAPGGDDVDATVVYGVNDSVLTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGRLDGLAIRVPTVNVSLVDLSFQAARDTTVEEINALMKAASEAGPLKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMVKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
BASE COUNT         1102 a         1476 c         1011 g          781 t
ORIGIN      
        1 tgcgatttgc gaaagcgtta ttaaaccaaa taaccggaaa tggcgcaagc cttatttcgg
       61 catttcattt atattccctg caaactccag ttttcaaaag agataatttc ttcttttttg
      121 cctgcgacca ccaacacgtc accgccttcc aatgtaaacg aggcatccgg gttttcaatc
      181 cgaccggtgc ggcggcggac gaacaagagt ttgatgccgt aagccgccat cggaagcgtg
      241 ccgactgttt tgccgacggc atatgcttct gcagccaaag gaaaggcgtg gcggacggtt
      301 tcgccatttt cgccgaatcc tgcctcatca tcactaccga caaacagtcc ctccaacgcg
      361 gcataacggc tgtggcggat atttgccatc gtctgataga cgtgccgata cgacgcgccg
      421 ttgcccaaca tcgcatagcc tgccagcacg agtccggttt ctttggtgtc cgacacggct
      481 tcttccgcac ctatatcggt aaacgttttc acataatcgt cgttggtggc gcgcacatat
      541 acgggcatat tgggatacat ggacagcaca ttgtctaaaa cgtgttgcgt ttcgtgcata
      601 ttgttgagcg taaccaccac cattttcgcc cgtcccaaac cggccgcctc caacacttca
      661 cgccttttcg catcgccgaa cgacaccggc tcgccggcat ttttggcaac ctgcacgcgc
      721 gcgatgtcca agtcgagcgc gaaatacgga atatcctctt gggcaaggac gcgtccgacc
      781 gtctgcccgc ccctgccgaa gccgacaatc agcacatggt cggacttgct catggtttct
      841 accagcatac tgtgcagatc gagcgacttc atgtcccagc ttgacttgac caaacgcccg
      901 accagtgcat cgctgccgcc caagaggaag ggcgcgataa tcatcgacag cagaaccgcc
      961 gccgtcgccg cctgttccca ttctggcgaa accatatcaa gctgcccggc aatggccagc
     1021 atcacgaagc cgaactcgcc gccttgtgcg agatacaaag ccgttttgag gctgtcgccg
     1081 accgaatgtt tcattttgaa ggcaatggca aacacaacca gtgccttcaa caccagcagc
     1141 attgccaaca gcatcaatac ctgccgccag ccgccgatta atgcctgaat gtccagcttc
     1201 atgccgaccg tgataaagaa aaagccgagc aagatatcgc ggaacgggcg gatgtcgtct
     1261 tcgacttgga aacggtattc cgtttccgaa agcagcatcc ccgccacaaa cgcgcccaac
     1321 gccatagaca aaccttccag ctcagtcaga taagccacac ccaaggttac cagcagcaca
     1381 ttgatcataa agagttcgga cgatttgcgt tttgccacca tcctgaacca tcgcgacata
     1441 attttgctgc cgacgaaaaa cagcagcccc agcgtcagca gcatttttgc aaacgccaaa
     1501 cccaaggccg cccaaatatt tccgtcccct ccgcccgcca gcgcgggaat cagaatcatc
     1561 agcggcacga cggcgatgtc ctgcatcagc agcacgccca tcgccatctg accgtgcggc
     1621 tgccccaatt ccgtcttttc cgacaaaatc cggctcacaa tcgccgtgga cgacatcgcc
     1681 aacgcgcccg acacggcaaa cgcccaattg aacggcacgc ccgtcagcat cagtatgccc
     1741 attaccgaca gcatcgtaac gccgacctgc aaaccgccca gcccgaatac cagccgcctc
     1801 atcgccctca acttgggcag cgagaactcc aaaccgatgc tgaacatcag gaacacaatc
     1861 ccgatttcgc ccaaataatc cgtcgcatgg cttttcggaa tcaggctgag cataccgggc
     1921 cccgccaaaa agcccaccag caggtagccc agcatggagg gaatgttgaa cttgcggcac
     1981 aggatcaccg taatgaccga caccagcaaa acaaccacaa taggggcaag cgaaaattcg
     2041 ttcataggcc gtccgaacag gaaaatacag aaaaatgccg tctgaaacgg catacgccgc
     2101 cgtattataa caaaacaccg cgcaccatcc gaaacgggcg cggcatacaa ttctgctaaa
     2161 atacgccctt tcgattttga gccgcacacc gacatgaccg ccacagccgc cgccatagac
     2221 cgcctgcttc cccaaaccca atgccgcgaa tgcggctacg acggctgcct gccctacgca
     2281 caggcagtcg caacaggcga agcgtacaac ctctgcgccc cgggcggcgc aaccgtcgtc
     2341 cgcgaccttg ccgccctgct cggcaaaccc cttgtcgcac ctgccaaaac ccaagccaaa
     2401 gcactcgccc ggatagacga atccgcctgt atcggctgca ccgcctgtat ccgcgcctgc
     2461 cctgccgatg ccattatggg cgcgggcaaa cttatgcaca ccgtcatcgc cgacgaatgc
     2521 accggctgcg gactctgcgt cgccccctgc cccgtcgact gcatccatat gcagcccgtt
     2581 gccgacaccg tcctgccccg cgcgcgccgc ttcagcctgt ccgccgacag ccgttttgcc
     2641 gccgccgaac acgcgcgcgc gcgctacctc aaacgcaacg aacgcaaaca gcgcgaagcc
     2701 gacgaacgca aggccatgct tgccgaacgc gaagccgccg tccgcaacgc gcgtccgcaa
     2761 acacccgaca caccgaaaaa accggcgttt aaccctgccg acctcatcgc caaagccatg
     2821 gcaaaagcgc aaacccaaca agaccgcctc gccgccgccg acaaccgcaa agactatcag
     2881 gcgaaacaga tagccgaagc ccgcgaacgc gccgagttgc gccgcgccca acgcgatatg
     2941 aaatacggca gcgacagcga aaaagccgcc gccctcgaat acctcaaaca atacaaagcc
     3001 aaacaggaag ccgcacagaa taccgcctcc tgacccttcc ccgacatgcc gtctgaagcc
     3061 gcttcagacg gcattttatc aagctctccg tccgccgcac ccgtgccgtc cgcatcttac
     3121 cgcccaccct tccggccgcg ccgttttcaa taaaatatta attacacgcc actacaaatt
     3181 tgctataatc cgccccgaaa atctacccaa ccctcaacaa aggaacaaac catgggcatc
     3241 aaagtcgcca tcaacggcta cggacgcatc ggccgccagg ttttgcgcgc catctacgat
     3301 tatcagattc aagaccaact ccaaatcgtc gccgtcaacg ccagcggcag ccttgaaacc
     3361 aacgcccacc tcaccaaatt cgacaccgtg cacggacgct ttgaagccga cgtatcccac
     3421 gacggcggca acctcatcgt caacggcgac aaaatcccct tcttctcgac ccgcaaccct
     3481 gccgaactgc cgtggaaaga actcggtgtc gatttggtca tggaatgcac cggcgcgttc
     3541 accagcaaag aaaaagccaa aatccacctc gaaagcggcg cgaaaaaagt cctcatttcc
     3601 gcaccgggcg gcgacgatgt cgatgcaacc gtcgtgtacg gcgtgaacga cagcgtcctg
     3661 accgccgaca tgaccgtcgt ttccaacgct tcctgcacca ccaactgcct ctcgccggtt
     3721 gccaaagtgt tgagcgaaag cgtcggcatc gtcaaaggcg cgatgaccac catccacgcg
     3781 ctgaccaacg accaaaccgt taccgacgtg cgccacaaag acctgcgccg cgcccgcagc
     3841 ggcgtggaaa acatgattcc gaccaaaacc ggcgcggcaa aagccgtcgg tttggtactg
     3901 cccgaattga aaggcaggct cgacgggctt gccatccgcg tgccgaccgt caacgtatca
     3961 ttggtagatt tgagcttcca agccgcgcgc gacaccacag tcgaagaaat caacgcactg
     4021 atgaaagccg cctcggaagc aggcccgctc aaaggcgttt tgggctacaa caccctgccc
     4081 ttggtttcca tggacttcaa ccacactacc gaagccagcc acttcgacgc aacactgacc
     4141 aaagtcgttg acggcaacat ggtcaaagtg ttcgcttggt atgacaacga atggggcttc
     4201 agctgccaaa tgctgaacac cgcacgccgt atgttcggac ttgaagtgcg cccgctcaaa
     4261 taagcaacaa accgtcaaac aaaatgccat ctgaaacccg atgttttcaa gtttcagacg
     4321 gcatttttca ttttcaccgt gattttatcc ggctgtcgtc atttctaatt
//
LOCUS       DAWKOG010000162         4307 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.162, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000162 DAWKOG010000000
VERSION     DAWKOG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.162"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(153..398)
                     /locus_tag="WJK66_001834"
     CDS             complement(153..398)
                     /locus_tag="WJK66_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579498.1"
                     /translation="MLKSIELNSHIRNRLAEYLKGRGMDFQTAMQEEKGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            510..938
                     /locus_tag="WJK66_001835"
     CDS             510..938
                     /locus_tag="WJK66_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEZ7579499.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDVPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            950..1426
                     /locus_tag="WJK66_001836"
     CDS             950..1426
                     /locus_tag="WJK66_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEZ7579500.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GQRAWLTLKGYISELSRSEFEYEIPLADAEKMMETMCPFKMEKWRYPVRWGGSLFEID
                     VFLGDNAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSRHPFSSWKNAV"
     gene            complement(1776..2264)
                     /locus_tag="WJK66_001837"
     CDS             complement(1776..2264)
                     /locus_tag="WJK66_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEZ7579501.1"
                     /translation="MLKITLIAACAENLCIGADNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYYAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPAIDRTHWKEAERTERRVSSKGTSYAFVHYL
                     RY"
     gene            complement(2332..3387)
                     /gene="aroG"
                     /locus_tag="WJK66_001838"
     CDS             complement(2332..3387)
                     /gene="aroG"
                     /locus_tag="WJK66_001838"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HEZ7579502.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGGNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANKKRMARAS"
     gene            complement(3549..3776)
                     /locus_tag="WJK66_001839"
     CDS             complement(3549..3776)
                     /locus_tag="WJK66_001839"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579503.1"
                     /translation="MGNTRIPIKSFFKQLVLFVFNIFLAYEILPDVQAESAAGCSVPD
                     CRGNGKTDVPCGFVRMNGGYCFYQSVSFSLE"
     gene            4003..>4307
                     /locus_tag="WJK66_001840"
     CDS             4003..>4307
                     /locus_tag="WJK66_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579504.1"
                     /translation="MTPWADAYADLRGKTKVMTTQMCASRDVSKSAKGWSVGIGLNVG
                     KQLTDSVGLEFDPYYRHKTICKPREIVLDGDKTKMGRSKSNEYGFRVTATFYSQLKS"
BASE COUNT         1019 a         1115 c         1011 g         1162 t
ORIGIN      
        1 ccgcctgcgc gggaatgacg gctcaaaagt tacgaaacga aaaataaccg aaaccaaaca
       61 agtcggattc ccgcgcgggc gggaatgacg ataagcgtaa tttcaatctg ccatattgtc
      121 ttttcacttg cctgaaaaca gatttcagcc acctatccct gcatccggcg gctgatgtag
      181 tcggcaatca aatcccgctt ttcccaaaaa aacttctgca ttttttgttc ggaatacagt
      241 ttgcggacca gagtgggcaa accgctgtgg acgatggcgg cgatttcttt gttgcctttt
      301 tcttcctgca ttgccgtctg aaaatccata cccctgcctt tcagatattc tgcaaggcgg
      361 ttgcggatgt gggaattgag ttcgatggat ttgagcattt tgcctccgtt ttgcggtaag
      421 gatttattcg atttgtttat ccgcctgatt ttatcacgtt tcccaagcgg tttgcctgta
      481 caataccgct tttcggcagg gtgtgggcga tgatgttttc ttggttcaag ctgtttcact
      541 tgttttttgt catttcgtgg tttgcagggc tgttttacct gccgaggatt ttcgtcaata
      601 tggcgatgat tgatgtgccg cgcggcaatc ccgagtatgt gcgtctgtcg ggcatggcgg
      661 tgcggctgta ccgttttatg tcgccgttgg gcttcggcgc ggtcgtgttc ggcgcggcga
      721 taccgtttgc cgccggctgg tggggcagcg gctgggtaca cgtcaaactg tgtttgggct
      781 tgatgctctt ggcttaccag ttgtattgcg gcgtgctgct gcgccgtttt caggattaca
      841 gcaatgcttt ttcacaccgc tggtaccgcg tgttcaacga aatccccgtg ctgctgatgg
      901 ttgccgcgct gtatctggtc gtgttcaaac cgttttgacg gagcatctta tgccgataga
      961 aatcgaacgc cgttttttga ttggaaacga caactggcgg caatacgccg acgaacctct
     1021 gctgttgagg cagggttatc tgtctgttga aaaagaacgc accgtccgtg tccgtattgc
     1081 cggacaacgg gcgtggctga cgctgaaagg ctatatttcg gaactcagcc gcagtgagtt
     1141 tgaatacgaa atcccgcttg ccgatgcgga aaagatgatg gaaacgatgt gtccgtttaa
     1201 gatggaaaaa tggcgttatc cggtcagatg gggcggcagc ctgtttgaaa tcgatgtttt
     1261 tcttggcgat aatgcgcctt tggtcgtcgc ggaaatcgag ttgcccgacg agaatgccga
     1321 ttttgaccgt ccggactggc tgggacggga aatcactgcc gacggtatgt ttaccaatgc
     1381 ctatttgagc aggcatccgt tctcaagctg gaaaaatgcc gtctgaagcg gcttcagacg
     1441 gcattctccg acaaccatcg caaatccaaa gacagcctct atatccttat ttctgctttc
     1501 tttgttccat tattcccaat aaatccccat aacctaaaat ctcgtcattc ccgcgcaggc
     1561 gggaatccgg ttctttaaac ttcggttttt tccgataaat tgctttagct tttcgtttcc
     1621 ggattcccgc ttttgcggga atgacgatgg aagattgttg ttatttcgga taaattaccc
     1681 taactttaaa atcccggatt cccatcttcg ggggaataac aatattttct atttcagccc
     1741 atcatttgcg ggggatttta tagagagttt atatttcaat atctcaaata atgcacaaaa
     1801 gcatagctcg tgcctttgct gctgacacgg cgttccgtcc gctctgcttc tttccaatgc
     1861 gtccggtcta ttgcggggaa aaatgcatct ccttccacag acaaatccac ttcggttatc
     1921 cgcaaatcgg tcgccaatgg catcgcttgt ccgtatatct gcgcgccgcc cataatgacg
     1981 gcttcttccg cgcctgcgca caatgccaat gccgcctcca aacttgccgc cgtttccgcg
     2041 cctgccgcgt aataatccgc ctgccggctg atgacgatgt tcctccgtcc gggcaggggt
     2101 ttgacgggca gggattccca cgttttccgc cccataatga cgggtttgcc caaggtatag
     2161 gctttgaaaa atgcgaaatc ttcggggatg tgccaaggca tagcattgtc cgccccgatg
     2221 cacaggtttt ccgcacatgc cgcaattagg gttattttga gcatatttcc tccacaaaaa
     2281 tgccgtctga acgcttcaga cggcatttta tgacattctg cgtcaaaaat ctcaactggc
     2341 gcgcgccata cgttttttgt ttgcacctgc caacaatgcc aacagttctt cagtcgcgcc
     2401 ccaaccgata cacgcatcgg taatgctttt gccgtacact tccggcttgt cctgcctgcc
     2461 ttcgaccaaa tggctttcca ccatcacgcc catgatattg ccgccgtcct gttccaattg
     2521 ggcggcaata tcttgtgcca cttccatctg ccgggtgtaa tccttgcggc tgttggcgtg
     2581 gctgcaatct atcatcagct tgtcggttac ccctgccgca cgcagttgtt ccgccgcctc
     2641 gctgacgtgt cccgcatcat aattcggctc ttttccgccg cgcaaaatga catgacagtc
     2701 aggattgccg ccggtatgga caatggcgga atgcccggcc ttggttacag acaggaaatg
     2761 atgcgaatgg ctcgccgcac cgattgcgtc gatggcaatc ttcaaattgc cgtccgtacc
     2821 gtttttaaag ccgacggggc aggacagccc gcttgccaat tcgcggtgaa cttggctttc
     2881 ggtcgtccgc gcaccgattg ccccccaaga aatcaagtcc gcataatatt gcggcgtaat
     2941 catatccaaa aactcggtag aggcaggcat acccatattg ttcagcgaca acaacaggct
     3001 gcgcgcctga cgcaaaccga aattgatgtc aaacgtaccg tccaaatgcg ggtcgttaat
     3061 caaacctttc caacccaccg tcgtcctcgg cttctcgaaa taaacgcgca tcacaatcaa
     3121 aagctcgttt tcatactgct tgcggagttt caacaaacgc tccgcatatt ccaacgccgc
     3181 tttcggatcg tgaatcgaac acggcccgat aataaccaac agccgcttgt ccctgccgtg
     3241 aaccaaatcg gaaatttcct gacgggtgcg gtgaaccaag cccgaagcct ctttggaaat
     3301 cggcagctcg taaagatggg caatcggcgg caacaactct ttaacttctt taatcttaat
     3361 atcgtctgtg gggtaatggt gtgtcatctt gcatctttcc tgatggtttt ttatacgcgg
     3421 cttaggttac atcgtttacc ttgcacaaac aagtgcaaca tattactaca aagctatttt
     3481 atttcaaaat aactgatata tcttttttca tagattttaa atttcatatt tttaatattt
     3541 taggaatttt attccaaaga aaaagaaaca gattgataaa aacaataccc gccattcatt
     3601 cggacaaaac cgcacggaac atccgttttc ccatttccac gacaatccgg tacagaacac
     3661 cccgctgccg attccgcctg cacatccggc aaaatttcat acgcaaggaa aatgttgaaa
     3721 acaaaaagaa ctaattgctt gaaaaaagat ttaattggaa tgcgggtatt tcccacactt
     3781 tctccattta ttcatcctca ttataggaag aaaaacatga aaaaaccctg cttgcactct
     3841 cacttgcagc cgtgtccgcc gttacggtag ccgatattcc tgagtctaac ggcaagggca
     3901 aagccatcat cggcgataag gaaaaacacc gttaccaagc atgaaaacct tgttttgaaa
     3961 gtctatggcg aaatccgcca agatttgggg gacggatgat ccctgacccc ttgggcggat
     4021 gcatatgcag atttgcgcgg caaaaccaaa gtgatgacga cccagatgtg tgcttcccgc
     4081 gatgtcagca aaagcgccaa aggttggagt gtcggtatcg gtctgaatgt aggcaaacag
     4141 ttgaccgaca gcgtcggtct cgagtttgat ccatactacc gtcacaaaac aatctgcaaa
     4201 ccccgtgaga ttgttttgga cggcgacaaa accaaaatgg gccgctccaa atccaacgag
     4261 tacggcttcc gcgtaaccgc aacgttctat agtcaattaa aatcaaa
//
LOCUS       DAWKOG010000163         4252 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.163, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000163 DAWKOG010000000
VERSION     DAWKOG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.163"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(122..1084)
                     /locus_tag="WJK66_001841"
     CDS             complement(122..1084)
                     /locus_tag="WJK66_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ7579505.1"
                     /translation="MRNAVGLDISKLTFDAFAMVGNAEYSAKFDNNSKGLNQFSDWLK
                     SLGCQNLHICMEATGNYYEEVADYFAQYYSVYVVNPLKISKYAESRFKRTKTDKQDAK
                     LIAEYCRSAQESELVKRQKPTDEQYRLSRMTVAYTQIKSECAAMKNRYQVAKDEEAAK
                     AYAEIIKAMNEQLEVLKAKIKEQTEKPKCKEGVKCLETIPGIGRMTAAVLFHHLTSSQ
                     FETSNKFAAFAGLSPQQKESGTSVRGKGKLTKFGNRKLRAVLFMPAMVAYRIRAFPDF
                     IARLEAKKKPKKVIIAALMRKLAVIAYHVHKKGEHYDPSRYKSA"
     gene            complement(1598..1957)
                     /locus_tag="WJK66_001842"
     CDS             complement(1598..1957)
                     /locus_tag="WJK66_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579506.1"
                     /translation="MINKPRYIKIVDENGDFTRVLRLHKFPDTSKVFYFEPMFWLKDG
                     RVARKDSLFEVDYIYGADGCGFLPSNLTEFKKYCRKQYQKFKDDEVLVNRYAVDFLGA
                     KEPPYDDRHVTSVKYFV"
     gene            complement(2078..3163)
                     /locus_tag="WJK66_001843"
     CDS             complement(2078..3163)
                     /locus_tag="WJK66_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HEZ7579507.1"
                     /translation="MIYLFTGNMGTGKTSRVVSMILNNEDGLFKMKLEDGTEVDRPLY
                     FCHIDGLDKRQFKAHELTEEQIMSAPLRDVIPEGAVLIVDEAHYTYPVRAAGRPVPPY
                     IQELTELRHHGHTVILMTQHPSQLDIFVRNLVSKHVHLERKAIGMKQYSWYKCVTSLD
                     NPAGVSGVEVASWKPPKEAFKYYKSASQHQKFKKKVPWAVWALIAIIGFIGWKSFGIF
                     KVYSKATDSRIEQEAQKESVVQTMTEQPASSEEMPLKNSDNLKPEDFVPTLPEKPESK
                     PIYNTVRQVKTFEQIAGCIDGGKSDCTCYSNQGTPLKEITKIMCKEYVKNGLPFNPYK
                     DEQQRTEQVEQSAKADKPQVLVMGGKP"
     gene            complement(3173..3463)
                     /locus_tag="WJK66_001844"
     CDS             complement(3173..3463)
                     /locus_tag="WJK66_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HEZ7579508.1"
                     /translation="MPLLAGLIPLLGILLKMLIVRIILATGLTFVTYAGYLIALEKFK
                     DYTSNAINSMPSDILNLLLISGFGQGLGYLFGAFSFFIGMHAFKKLTFVFPG"
     gene            complement(3464..>4252)
                     /locus_tag="WJK66_001845"
     CDS             complement(3464..>4252)
                     /locus_tag="WJK66_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ7579509.1"
                     /translation="AKKLEEILSLKVDANPDKYIKATGYPGYSEKVEVAPGTKVNMGP
                     VTDRNGNPVQVVATFGRDSQGNTTVDVQVIPRPDLTPGSAEAPNAQPLPEVSPAENPA
                     NNPNPNENPGTSPNPEPDPDLNPDANPDTDGQPGTRPDSPAVPDRPNGRHRKERKEGE
                     DGGLLCDYFPEILACQEMGKPSDGMFHDISIPQVIDDKTWSSHNFLPSNGVCPQPKTF
                     HVFGRQYQASYEPLCVFAEKIRFAVLLAFIIMSAFVVFGSLKGK"
BASE COUNT          998 a          999 c          922 g         1333 t
ORIGIN      
        1 aattgacaat agcatcttta caattcattt tcaactcatt gaatttcaat atttaaaaat
       61 tcactggatt tcagacacgg caaagcaggc atttaaaaaa tgcctttttt cctttcagga
      121 tttacgccga tttgtaacgg gatggatcgt aatgctcgcc tttcttgtgc acgtgatacg
      181 caatgacggc aagtttacgc atcaatgcgg cgatgatgac ttttttaggc ttcttcttcg
      241 cttccagcct tgcaataaaa tcaggaaatg cccttatgcg gtatgcaacc atggcaggca
      301 taaacaagac ggcgcgtaat ttcctgttgc caaacttggt cagtttacct tttcctctta
      361 cgcttgtgcc tgattctttt tgttgcgggc ttaggcctgc gaacgctgca aatttgtttg
      421 atgtttcaaa ctgcgaagat gtgagatgat gaaacaatac ggctgcggtc attcttccaa
      481 ttcccggtat ggtttcaaga catttcacgc cttccttgca tttcggcttc tccgtctgct
      541 cttttatctt cgcctttaaa acttcaagct gttcattcat ggctttgatg atttctgcat
      601 atgctttagc cgcttcttca tcttttgcca cctgataacg atttttcatt gccgcgcatt
      661 cgcttttgat ttgcgtatat gctaccgtca tccgtgaaag cctgtattgc tcgtctgtcg
      721 gcttttgcct ttttacaagc tcgctttcct gcgccgaccg gcaatactcc gctatcagtt
      781 ttgcatcctg tttgtctgtt ttggtgcgtt tgaatctgct ttctgcatac ttgcttattt
      841 tcagcgggtt cactacgtaa acgctgtaat actgcgcgaa gtagtcggca acttcttcat
      901 aatagtttcc cgttgcctcc atacagatat gcagattctg acatcccaag cttttcaacc
      961 agtccgaaaa ctgatttaaa ccttttgaat tgttgtcaaa ctttgccgaa tattcggcat
     1021 tgccgaccat ggcgaatgcg tcaaatgtca gctttgatat atccaatcct acggcgttac
     1081 gcatgggatt acccttatct attcagaatc tatgttcttt gatactactc aatttcacaa
     1141 acaagaaaac cgcccgccta ttctcgtcat caaactttaa gtttgtggtt tattcagact
     1201 ggacggttgc ggcaaagggt agctattcct ttgccgtgtc tgattttatt tgggttgcag
     1261 gttttggtaa aggtttctgt tgcgacccga atgtctgatt ttttttgggc gtatctcatt
     1321 ctagaatcac tccgttagtg ggtttccggt attgaaaaac agttcataaa aaaggaaaag
     1381 ggggtattca taaagattgg gtaaaaagcg cgcccaatct ttacaaagct tccccctttt
     1441 ccttttttct gccctatttt cctgcaccta caacccccga acgaagcgat tctagactga
     1501 gacactttaa aaaaaacagc cattctagca gttaaccccc ttcactcagc tcaagccatc
     1561 ctgaggtata gtggctgaat ttgtgatttt ggttttatca aacaaaatat ttgactgaag
     1621 tcacatggcg gtcgtcatat gggggttcct ttgcacccaa aaaatcgacc gcgtaacggt
     1681 ttactaaaac ttcatcgtcc ttaaactttt ggtactgttt ccggcaatat tttttgaact
     1741 ccgttaaatt tgatggcaag aatccgcaac cgtctgcacc gtaaatgtaa tcaacttcaa
     1801 acaaactgtc tttccgggca acccgaccat ctttaagcca gaacataggc tcgaaataaa
     1861 aaactttcga cgtgtccgga aacttatgaa gacggagaac acgtgtgaaa tccccgtttt
     1921 catctacaat tttgatatat cttggtttgt taatcatgac atcctcagat ttagtattca
     1981 gaatatgatt ttaaaaagaa ctttctgctt tacgactccg ccgccaattc cttcaaacgg
     2041 ttttccgcgc tcttcagttg tcgtacatga gattttgcta cggctttccg cccattacga
     2101 gaacttgcgg cttgtccgct ttcgcggact gttccacctg ttccgtcctt tgctgttcgt
     2161 ccttataagg attgaaaggc aacccgtttt tcacatattc tttacacatt atctttgtta
     2221 tttctttcaa gggtgttcct tgatttgaat agcatgtgca atctgatttt ccgccgtcta
     2281 tacatccggc gatttgctca aaggttttta cttgtcggac tgtgttataa ataggcttgc
     2341 tttcgggctt ttcgggtaaa gtcggcacaa agtcttcagg tttcaaattg tctgaatttt
     2401 ttaaaggcat ttcctctgat gatgcaggct gctccgtcat cgtctgcaca acgctttctt
     2461 tttgcgcttc ctgctcaatc cggctgtctg tggctttgct gtaaacttta aaaatgccga
     2521 aacttttcca gcctataaac cctataatcg caatcaacgc ccaaaccgcc caaggtactt
     2581 ttttcttaaa cttttggtgt tggcttgcgg atttatagta tttgaaggct tctttaggcg
     2641 gtttccaact tgcgacttcc acgccgctta cacctgccgg attgtccaac gaggttacgc
     2701 atttatacca agaatactgt ttcatgccga ttgccttgcg ttcaaggtgt acatgctttg
     2761 aaacaaggtt gcggacaaat atatcaagtt ggctcgggtg ctgcgtcatc aaaataacgg
     2821 tatgcccgtg atggcgtaat tctgtcagtt cttgaatata gggcggaacg ggacggcctg
     2881 ccgcgcgtac cgggtaagtg tagtgcgctt cgtcaacaat cagcaccgcg ccttccggta
     2941 tgacatcacg aagcggggcg gacatgattt gctcttccgt cagttcgtgg gctttaaact
     3001 gccgtttatc caatccgtcg atatggcaga aataaagcgg cctgtctacc tctgtgccgt
     3061 cttccaattt cattttgaac aatccgtctt cgttgttcaa aatcatagag acgacgcggg
     3121 aggttttgcc tgtccccatg tttcctgtaa acagataaat catgcttcta cctcatcctg
     3181 gaaagacaaa cgtcagtttt ttgaatgcgt gcataccaat gaagaacgag aatgcgccga
     3241 acaggtagcc caaaccctgc ccgaatcccg aaattaaaag aaggttcagt atgtcggaag
     3301 gcatggaatt aatcgcattt gacgtgtagt ctttgaactt ttccagcgcg atgagataac
     3361 cggcataggt tacaaatgtc agacctgttg caaggatgat tctgacaatc agcattttca
     3421 gaagtatgcc taaaagtgga atcagaccgg caagtaatgg catttatttc cccttcaacg
     3481 aaccgaaaac gacaaaagcc gacataatga taaaggcgag cagtacggca aaacggattt
     3541 tttcggcaaa cacgcacaac ggctcatagc ttgcctgata ttgcctaccg aaaacatgaa
     3601 aggttttcgg ctgcggacat acgccgttag atggtaaaaa gttatgtgaa gaccatgttt
     3661 tatcgtctat aacctgcggt atgcttatat cgtgaaacat gccgtctgaa ggtttgccca
     3721 tctcctgaca ggctaggatt tccggaaaat aatcgcacaa aagcccgccg tcttcgcctt
     3781 ctttcctttc tttgcgatgc ctaccgtttg ggcggtccgg aacggcgggg gaatcgggtc
     3841 ttgtgccggg ctgtccgtcc gtatcgggat ttgcatcggg attcaaatcg gggtcgggtt
     3901 cgggattggg gctcgtgccg gggttctcat tggggttcgg gttgtttgcg gggttttcgg
     3961 cgggcgatac ttcgggcagc ggctgtgcgt tcggtgcttc cgcgcttccg ggtgtgaggt
     4021 cgggacgcgg gattacttga acatccaccg tggtgttgcc ttgcgaatcc ctgccgaatg
     4081 ttgcgacaac ctgaactgga ttcccgttcc tgtccgtgac gggacccata ttcacttttg
     4141 ttccgggtgc gacttctact ttttcggaat aaccgggata tccggttgcc tttatgtatt
     4201 tgtcgggatt ggcatcgact ttcaacgata aaatctcttc cagctttttg gc
//
LOCUS       DAWKOG010000164         4175 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.164, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000164 DAWKOG010000000
VERSION     DAWKOG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.164"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            38..461
                     /locus_tag="WJK66_001846"
                     /pseudo
     CDS             38..461
                     /locus_tag="WJK66_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            567..1130
                     /gene="pgsA"
                     /locus_tag="WJK66_001847"
     CDS             567..1130
                     /gene="pgsA"
                     /locus_tag="WJK66_001847"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEZ7579510.1"
                     /translation="MPWNLPIFLTWLRVLLIPVMTALFYLPFPWFAEETINLTAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NVLMFIASLLTVWSMLYYLKMAWKEIA"
     gene            1210..1285
                     /locus_tag="WJK66_001848"
     tRNA            1210..1285
                     /locus_tag="WJK66_001848"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1243..1245,aa:Gly,seq:gcc)
     gene            1311..1386
                     /locus_tag="WJK66_001849"
     tRNA            1311..1386
                     /locus_tag="WJK66_001849"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Gly,seq:gcc)
     gene            1410..1485
                     /locus_tag="WJK66_001850"
     tRNA            1410..1485
                     /locus_tag="WJK66_001850"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1443..1445,aa:Gly,seq:gcc)
     gene            1507..1582
                     /locus_tag="WJK66_001851"
     tRNA            1507..1582
                     /locus_tag="WJK66_001851"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1540..1542,aa:Gly,seq:gcc)
     gene            1608..1681
                     /locus_tag="WJK66_001852"
     tRNA            1608..1681
                     /locus_tag="WJK66_001852"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1640..1642,aa:Cys,seq:gca)
     gene            1784..1873
                     /locus_tag="WJK66_001853"
     tRNA            1784..1873
                     /locus_tag="WJK66_001853"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1818..1820,aa:Leu,seq:taa)
     gene            1977..3029
                     /gene="sohB"
                     /locus_tag="WJK66_001854"
     CDS             1977..3029
                     /gene="sohB"
                     /locus_tag="WJK66_001854"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEZ7579511.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSSETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(3080..3943)
                     /locus_tag="WJK66_001855"
     CDS             complement(3080..3943)
                     /locus_tag="WJK66_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HEZ7579512.1"
                     /translation="MENVETHSQNHRKRLIGTFFLVGLENLLMLVYPVFGGWAINAVI
                     AGQAWQALLYALVVLLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAVT
                     ARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLPR
                     FAAISENLYFRLKNSLERDNHFIRKGDERQLDRHYGLLARLRVLISNREAFGYLCVGT
                     AMGILFGFAFVMMTLKGYSSAGHVYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQRI
                     EWSKRNIKAGT"
     gene            complement(3956..4075)
                     /locus_tag="WJK66_001856"
     CDS             complement(3956..4075)
                     /locus_tag="WJK66_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEZ7579513.1"
                     /translation="MQRSHKVFATFGDKRTTRLFADLDALAEVMEKTISENKK"
BASE COUNT         1051 a         1005 c         1071 g         1048 t
ORIGIN      
        1 tcgctgggta tggtttgccg ccataagctg tctgctgttg tccgccgttt ttattgcacc
       61 ttacctgacg gcatttcacg aacaagaaaa aacattcgaa tatgcggaat tgaccgttac
      121 cgcccccaac cgcagcggac gggcaatcaa actggatgca gacgggcagc attaccggct
      181 ttcctgctat gggttcgaca gtttgtgcac aggcggcaat atcggcagaa ccatcagggc
      241 ggagcaggtt agagccgttt taagcgaaac cgtcggcaaa ggttttttaa acggcgttct
      301 gttggaatac cgcaacagtg gcagtgtcta taagcaataa agattttgcc cgcacggaag
      361 accgccttgt cgaagtgttg gcacaacctg ccgttttctg cctgaaactg ggtattttgc
      421 ttttattgcc cgccattttc ctgcgtttga aaagaatgtg aaaacagatt ggcggggcgg
      481 gggaatgccc atgcagcaat tttacgtttt gggttatgag gataaccgtt ataatcacaa
      541 ctttacagtc tgcacagaga aaactgatgc cttggaatct ccctatcttc ctcacatggt
      601 tgagggtctt gctcattcct gtcatgacag ccctttttta cctgcctttt ccgtggttcg
      661 cggaggaaac gattaatctc accgccgccg tcatttttgc cgttgccgcc ttaaccgact
      721 ggtttgacgg atttttggca agactgtgga aacagacttc cgatttcggc gccttcctcg
      781 atcccgtcgc cgacaagctg atggttgccg tatcgctact tttactggtc aaactcgacc
      841 ggacctatgt tttgtttgcc atgatcatta tcggcaggga aattaccatt tccgcattgc
      901 gcgaatggat ggcgcaaatg ggcaaaagga acagcgttgc cgtcgccacc gtcggtaagt
      961 tcaaaaccgc cgcgcaaatg ctggcaattt tcctgctgct gctgaatatt cccgattttt
     1021 acggatttaa tcttgttgtt atcggcaatg tattgatgtt tatcgcatct ttgctgacag
     1081 tctggtcgat gctgtattat ctgaaaatgg cgtggaaaga aatcgcctga aaaaaacata
     1141 aaaatagctt gacggcaaaa acagaatcca taataattgc gtctcttcga tgttggagaa
     1201 tgaaatcggg cgggaatagc tcagttggta gagcgcaacc ttgccaaggt tgaggtcgcg
     1261 agttcgagac tcgtttcccg ctccaaaaat ttcattttcc cacaatcatt gcgggaatag
     1321 ctcagttggt agagcgcaac cttgccaagg ttgaggtcgc gagttcgaga ctcgtttccc
     1381 gctccaagtt ttatttcaaa catatcaacg cgggaatagc tcagttggta gagcgcaacc
     1441 ttgccaaggt tgaggtcgcg agttcgagac tcgttttccg ctccaaaaac cgatatgttt
     1501 gaacgcgcgg gaatagctca gttggtagag cgcaaccttg ccaaggttga ggtcgcgagt
     1561 tcgagactcg tttcccgctc caagtttttt agataagcca gttttagggc gagatagcaa
     1621 agtggttatg cagcggattg caaatccgtc tacgccggtt cgattccgac tctcgcctcc
     1681 attatcgtac tacggcgggg tggcagagtg tttatgctat gaggagtgca atcttcatat
     1741 aggccggtta aaatccgcgc ccccgcctcc acctttcaca aatgcccggg tggtgaaata
     1801 ggtagacaca acggacttaa aatccgtcgg gactaaacat cccgtgccgg ttcgattccg
     1861 gctccgggca ccaagctgaa aatgacaatg ccgctccaag cggttatttt tttatctgtc
     1921 ggacggtgat ggacaatatt gacatgttca tgcctgaaca agaggaaatc caatcaatgt
     1981 ggaaagaaat tttactgaat tacggtattt tcctgctcga actgcttacc gtgttcggcg
     2041 caattgcgct gattgtgttg gctatcgtac agagtaagaa acagtcggaa agcggcagtg
     2101 tcgtactgac ggatttttcg gaaaattata aaaaacagcg gcaatcgttt gaagcattct
     2161 ttttaagcgg ggaagaggca aaacatcagg aaaaagagga aaagaaaaag gaaaaggcgg
     2221 aagccaaagc agagaaaaag cgtttgaagg agggtgggga gaaatcttcc gaaacgcaaa
     2281 aatcccgcct ttttgtgttg gattttgacg gcgatttgta tgcacacgcc gtagaatcct
     2341 tgcgtcatga gattacggcg gtgcttttga ttgccaagcc tgaagatgag gttctgctta
     2401 gattggaaag tccgggcggc gtggttcacg gttacggttt ggcggcttcg cagcttaggc
     2461 gtttgcgcga acgcaatatt ccgctgaccg tcgccgtcga taaggtggcg gcgagcggtg
     2521 gttatatgat ggcgtgtgtg gcggataaaa ttgtttccgc tccgtttgcg attgtcggtt
     2581 cggtgggtgt tgtagcggaa gtaccgaata tccaccgcct gttgaaaaaa catgatattg
     2641 atgtggatgt gatgacggcg ggcgaattta agcgcacggt tacttttatg ggtgaaaata
     2701 cggaaaaggg caaacagaaa ttccgacagg aactggagga aacgcatcag ttgttcaagc
     2761 agtttgtcag cgagaaccgc cctcaattgg atattgagga agtggcaacg ggcgagcatt
     2821 ggttcggtcg gcaggcgttg gcgttgaact tgattgacga gatttcgacc agtgatgatt
     2881 tgttgttgaa agcgtttgaa aacaaacagg ttatcgaagt gaaatatcag gagaagcaaa
     2941 gcctgatcca gcgcattggt ttgcaggcgg aagcttctgt tgaaaagttg tttgccaaac
     3001 ttgtcaaccg gcgggcggat gtgatgtagt ttgccgaacg gatggaaatg ccgtctgaag
     3061 cgtgttcaga cggcattttt caagttccgg ctttgatgtt ccgtttcgac cactctatcc
     3121 gttgtccgat gtctttcaaa ttggaatatt gttcgaccaa tcgcggcacg tcgtccaaac
     3181 tcatggcaaa catccacaga taagtgccga ccgaatagac atgccccgcg ctgctgtagc
     3241 ctttgagcgt catcatcaca aaagcaaagc cgaacaaaat acccatcgcc gtgccgacgc
     3301 agagatagcc gaaggcttcg cggttggaaa tcagcacacg caggcgcgca agcagtccgt
     3361 aatggcggtc cagctgccgc tcgtcgcctt ttcggataaa gtggttgtcg cgttccaagc
     3421 tgttcttcag gcggaaatac aggttttcgc tgatggcggc aaaacgtggc aaaagccata
     3481 aaaacaacgc aagtatgccc accgccgaca cgccgaccca aaattccagc accagcagca
     3541 tgatgcacgc gccgaatatg gatacgacgg atgtcgcggc aatcggcagg tgttcttcaa
     3601 aaaagctgac aaactcacgc gacagggcaa cccgcgcagt taccgccgaa tgcgggactt
     3661 gccgctgccg ctgttccaac acaaccggca cggcgatttc ggtataaatc cgcgtaaacg
     3721 tgcgcgtatc ggcaatccgc cgcgccgcac cgaccagcca catcaaaagc acaaccaaag
     3781 cgtacagcaa cgcctgccac gcctgccccg caatcacggc attaatcgcc cagccgccaa
     3841 acaccggata caccagcatc aaaaggtttt ccagtccgac cagaaaaaat gtgccaatca
     3901 atcgcttgcg gtggttttgg ctatgtgttt caacattttc cacatatttg cccccctatt
     3961 ttttattttc cgagattgtt ttttccatca cttcagccag tgcatccaaa tcggcaaaca
     4021 gccgggttgt gcgcttgtcg ccgaatgtgg caaatacttt gtgagacctt tgcaaaattc
     4081 ctttccctcc cgacagctga cacccaaaca caggttttcg gctgttttcg tttcaaatat
     4141 ccactaattt taccccaata cccccctaat cctcc
//
LOCUS       DAWKOG010000165         4128 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.165, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000165 DAWKOG010000000
VERSION     DAWKOG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.165"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            54..581
                     /locus_tag="WJK66_001857"
     CDS             54..581
                     /locus_tag="WJK66_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEZ7579514.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLTELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            669..1724
                     /gene="plsX"
                     /locus_tag="WJK66_001858"
     CDS             669..1724
                     /gene="plsX"
                     /locus_tag="WJK66_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEZ7579515.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTTQVVGMDESPQSALKNKKDSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENVGGL"
     gene            1860..2111
                     /locus_tag="WJK66_001859"
     CDS             1860..2111
                     /locus_tag="WJK66_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ7579516.1"
                     /translation="MKTLEKRMKALDKRMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDVRLTEQESREISFINKEFEIESTSD"
     gene            2361..2552
                     /locus_tag="WJK66_001860"
                     /pseudo
     CDS             2361..2552
                     /locus_tag="WJK66_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239330.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2572..2775
                     /locus_tag="WJK66_001861"
     CDS             2572..2775
                     /locus_tag="WJK66_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579517.1"
                     /translation="MPSEACLVFRRHFLRLKQRIGNPALPVHGNLHLKTICIPNHTPI
                     PISVICTLPVLGNHRFFAYNSRI"
     gene            2802..3764
                     /locus_tag="WJK66_001862"
     CDS             2802..3764
                     /locus_tag="WJK66_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEZ7579518.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALDAAGLNGNEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSASDTPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            3811..>4128
                     /locus_tag="WJK66_001863"
     CDS             3811..>4128
                     /locus_tag="WJK66_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEZ7579519.1"
                     /translation="MKRQAFFKLMACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWAGAYLGSRIFRHKTAKKRFVVLFRLTVSGNVLA
                     TLIL"
BASE COUNT         1099 a         1200 c          981 g          848 t
ORIGIN      
        1 ggtttttgtt aatccgctat attccaaaag ccccaaccac caaggaaatt ccgatgaaac
       61 agaaaatctg gtacacctac gatgacatcc accgcgtcat caaagcattg gcagaaaaaa
      121 tccggaacgc cgacatcaaa tacgatgcca tgattgccat cggcggcggc ggctttattc
      181 cggcacgtat gctgcgctgt tttctggaaa ttccgattta tgccgtaacc accgcctatt
      241 acgacagcga caacgaagga caggttaccg aagaagtcaa aaaagtccaa tggctcgacc
      301 ccgttccaga agccctgcgg ggcaaaaacg tactcgtcgt cgatgaagtg gacgacagcc
      361 gcgtaaccat ggaattttgc ctgacagagc tgctcaaaga agacttcggt acgatcggcg
      421 tcgccgtact gcacgaaaaa atcaaagcca aagcaggcaa aatccccgaa ggcattccct
      481 atttcagcgg catcaccgta gaagactggt ggatcaacta tccgtgggac gcactcgaca
      541 tcgacgaaca caaccgcctt gccgaggccg gccgaggctg accctttcag acggcatatt
      601 ttccgaaccg atgccgtctg aagcccgcac gacccctgcc gcagaccgaa aacctaccgg
      661 agaaccctat gattacactt gccgtagatg ccatgggcgg cgaccaagga cttgccgtta
      721 ccgtacccgg cgcaaccgca ttcctccaag cacaccccga tgtccgcctg attatgaccg
      781 gcgacgaaac gcaactgcgc caagccctga ccgcggcagg cgcaccgatg gaacgcatcg
      841 acatctgcca taccacccaa gttgtcggca tggatgaatc ccctcagtcc gccctaaaga
      901 acaaaaaaga ctcctccatg cgcgttgcca tcaaccaagt taaagaaggc aaagcccaag
      961 ccgccgtatc cgcaggcaac acgggcgcac tcatggcaac cgcacgtttc gtcctcaaga
     1021 ccatacccgg tatcgaacgc cccgccatcg ccaaattcct cccgtccgac accgaccatg
     1081 ttacccttgc actcgacctt ggcgcgaacg tcgactgcac gcccgaacag ctcgcccaat
     1141 ttgccgttat cggcagcgaa ctcgtccacg cactccatcc tcaaaaagga cagccgcgcg
     1201 tcgggctggt caacgtcggc acggaagaca tcaaaggtac ggacaccgtc aaacaaacct
     1261 acaaactgct gcaaaacagc aaactcaact ttatcggcaa catcgaaagc aacggcatcc
     1321 tgtacggcga agcagatgtc gtcgtcgccg acggctttgt cggcaacgtc atgctcaaaa
     1381 ccatcgaagg cgcggtcaaa ttcatgagcg gagccatccg ccgcgaattc caaagcaacc
     1441 tgttcaacaa acttgccgcc gttgccgccc tacccgccct caaagggctg aaaaacaaac
     1501 tcgacccgcg caaattcaac ggggccatcc tgctcgggct gcgcggcatc gtgattaaaa
     1561 gccacggcgg cacagacgaa accggtttcc gctatgccct cgaagaagcc taccacgaag
     1621 ccaagtccgc cggcctttcc aaaatcgaac agggcgtagc cgaacaactc gccgcactcg
     1681 aaactgccaa agccgtccaa aacgaaaatg tcggcggtct gtaacacaca cgatgccgtc
     1741 tgaacgcccc cgcccctttc agacggcatc cgcccgcacc aaacctgcgg gcgcggacgg
     1801 cgatgcgcct gtccggcact tcccaaatac ttccctgtaa aataaggaat atttgaaaaa
     1861 tgaagacatt agaaaaacgg atgaaagctc tagacaaacg gatgatgaag ttcggaaaat
     1921 cccttgaagg caggcttgat gcccgtctga ttgaatccgc attggattat attcattatt
     1981 cggaacgttt tttggctttt gaaatcctgt gtacttatat cgaagatttc gatgtccggc
     2041 tgacggaaca agaatcccgg gaaatttctt ttatcaacaa ggaatttgag atagaaagca
     2101 cgtccgatta accaataaag ccaatgattt gataagaatg aaaacatcga cggtcgtttt
     2161 tggcggattt tttatggcag acaacggaga gcgaatccaa atccccgttt tggaaaatcc
     2221 tgacattagg gaaatcaatc acttttttcc gtatcaaatt ttgagaaaaa agccggcgtc
     2281 cttgttttca gaatcatccc cgagccggaa tttggcgata ccgaattaac tgtctatttt
     2341 gaaaaggata ttatttgcca atgattcaaa caattttaga agacggggat attgaagtca
     2401 aaaaccttaa aacggaaaat tacagtggga aggcaaggga aattttggga gattcctacc
     2461 ctatttagca tacctctaaa aatatttcta caattcaaaa tataatttct gaatttatga
     2521 tacaaaaaca aacaccggca ccaataattt gatggaaacc caacaatcaa aatgccgtct
     2581 gaagcatgtt tggttttcag acggcatttc cttcgcttaa aacagcgtat cggcaacccc
     2641 gccctgcctg tccacggcaa tctgcatctg aaaaccatct gtatcccaaa ccacaccccc
     2701 atcccgattt ccgtcatttg caccctgccc gtattgggca atcatcggtt tttcgcttac
     2761 aatagccgaa tctgaaccaa ctatccaaaa aggccgttcc catgcagtat gccaaaattt
     2821 ccggcacggg cagctatctt cccgccaacc gcgtcagcaa tgacgacctt gcccaaaaag
     2881 tggatacctc ggacgagtgg attaccgcgc gcacgggcat caaattccgc catattgcag
     2941 ccgaaaacga aaaaaccagc gaccttgccg ccgaagcggc gcgccgcgcg ctggatgccg
     3001 caggtttgaa cggcaatgaa atcgacctga ttatcgtggc gacggcaacg ccggatatgc
     3061 agtttccgtc tactgcgacc atcgtgcagc aaaaattggg cataaccaac ggctgccccg
     3121 cgtttgacgt acaggcggtg tgcgccggct ttatgtacgc gctgaccacg gcaaacgcct
     3181 acattaaaag tggcatggcg aaaaacgcgc tggtcatcgg cgcggaaacc ttcagccgca
     3241 tcgtcgattg gaacgaccgc acaacctgcg tattgttcgg cgacggcgcg ggcgcggtgg
     3301 ttttaagcgc gtcggacacg ccgggcatca tccacagcaa actcaaggcc gacggcaatt
     3361 atctgaaact cttaaacgtc cccgggcaaa tcgcctgcgg caaagtttcc ggttcgccgt
     3421 acatttcgat ggacggtccc ggcgtgttca agtttgccgt caaaatgctg tccaaaatcg
     3481 ccgatgacgt tatcgaagaa gccggttata ccgccgctca aatcgactgg atcgtgccgc
     3541 atcaggcaaa ccgccgcatt atcgaatcga ccgcgaaaca tttaggtttg agtatggaca
     3601 aagtcgtcct gaccgtccaa gaccacggca acacatccgc cgcctcgatt ccgctggctt
     3661 tggatacggg catccgcagc ggacaaatca aacgcggtca aaacctgctg ctcgaaggca
     3721 tcggcggcgg tttcgcgtgg ggcgcggtgc tgttgcaata ttgaacccga tgccgtctga
     3781 aacaggcttt cagacggcat ttcccatatc atgaagcggc aggctttctt caaactgatg
     3841 gcgtgtgcgg catttctgtc tgccgtttcg ctgcgcctcc ccgtattggg cgcgtgttac
     3901 gcaatattgt ccctctatgc gtttgcactt tacggcatcg acaaacggcg tgccgtgcgg
     3961 ggaaaacgcc gcattcccga acaccgcctg ctgctgcctg ccttgttcgg cggttgggcg
     4021 ggcgcatact tgggcagcag gatattcagg cataaaacgg cgaaaaagcg ttttgttgtg
     4081 ctgttccgtc tgactgtttc gggcaatgtc ctggcgaccc tcatcctg
//
LOCUS       DAWKOG010000166         4100 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.166, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000166 DAWKOG010000000
VERSION     DAWKOG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.166"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(222..1415)
                     /locus_tag="WJK66_001864"
     CDS             complement(222..1415)
                     /locus_tag="WJK66_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEZ7579520.1"
                     /translation="MNPKHLIAFSALFAATQANALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLQSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHNGNWQSLTSRNNSGDFPLGGILAHTGSGGSMTLTASDG
                     KTGKQRISLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTRAALPLTIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTHWSKWRADRMNDLAQTGADLVILSYGTNEAFGDNID
                     IADTEQKWLDTVRQIHDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQHMQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAKGYRRAAEMLADSLEELVRA
                     AAIRQ"
     gene            complement(1412..2380)
                     /locus_tag="WJK66_001865"
     CDS             complement(1412..2380)
                     /locus_tag="WJK66_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEZ7579521.1"
                     /translation="MKHFLSLFTSILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYAFSDGIKTFLSGETPPTAQDGGSADMPSEAVPQTGETEWKQD
                     TEAAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYPSFFDWP
                     KTIEETLQKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVERILEAAH
                     THRVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLKGKIILIPTAHTLSGGEDRYTDSV
                     NVNGKPVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            complement(2391..3827)
                     /locus_tag="WJK66_001866"
     CDS             complement(2391..3827)
                     /locus_tag="WJK66_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEZ7579522.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSVQNLLLLAAGMGWLYHI
                     GPVFAAIVVLYSSCVYLLGELLRSDRENTRRFWLGCGIAVSLTVLGFFKYFDFFRPLI
                     AQYAGQGGAIDILMPLGLSYYTFQSIAYLVYCFRAPHAARFEWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTRQPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLGGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RAANIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRNALCRLKYLAPLSWFVTFHFVCLTFVVFNTANPD
                     DAGAVFNALFANANGWNAPQRADMLLLASFASVMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIPVSAVLLLIIVFAPSGIPGFIYANF"
     gene            complement(3957..>4100)
                     /locus_tag="WJK66_001867"
     CDS             complement(3957..>4100)
                     /locus_tag="WJK66_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEZ7579523.1"
                     /translation="NVGIGSGALLGHWVTQYVGISRIGIAGMLAAAAGLWLCLNLNRH
                     IQT"
BASE COUNT          846 a         1039 c         1330 g          885 t
ORIGIN      
        1 ggaaggcggc agtttatcat ctttgccttc agacggcata tcaaacgccg cctttgcgcc
       61 gtatgccgga ggttcgcccc cgtccttcca atgcttgagg cgaatgccgg ttgcgtccgg
      121 caaaccgtgc gcggggtggc tatgccgtct gaagcaatat gaaggtatgc gtttcagacg
      181 gcatccccct gttgcggaaa taccgtccgc ctcctgtccg attattgcct gattgcagcg
      241 gcgcggacga gttcttcgag gctgtcggcg agcatttccg ccgcgcgccg gtagcctttg
      301 gcggagaagt gtacgccgtc tttggcggcc catccttggt tgagccagtt tttcatgctg
      361 catatgccgc ccatggcgtt ttgccaagac cagaacatgg tttgtccctg ccgggcgacg
      421 cgccgctgca tatgttggac ttcggtcagg cggacggggc gtgtgccgca tacgccgagc
      481 gtgtttttca gggattcggg tgcgccgatg atgaggatgc cggcggcagg caggctgtcg
      541 tggatttggc ggacggtatc cagccatttt tgttcagtgt cggcaatgtc gatgttgtcg
      601 ccgaaggctt cgttggtgcc gtaggaaagg ataaccaaat cggcgccggt ttgggcaagg
      661 tcgttcatac ggtcggcacg ccatttcgac caatgggtca attgtgcgcc gttgatgccc
      721 atggcggaaa cggtaatgcc gccggcggga ttttcgatgt tgatgaagcc gatgtcccac
      781 ggcatttcgg tctgtatggt caagggcagt gccgcgcggg tatccagtac ctgccagccg
      841 ccgccgttgg cggagacggt gttgccgttg acggtcaggg tttgttcggc gagcaggggt
      901 ttggcaaaca gggaaatgcg ctgtttgccg gtcttgccgt ccgatgcggt cagggtcatg
      961 ctgccgccgc tgccggtgtg ggcgaggatg ccgccgagcg ggaagtctcc gctattgttc
     1021 ctgctggtga ggctttgcca gttaccgttg tgccggacgg ccgccatgcg ctgccctttg
     1081 acgttggcgg ggtaaaccca gcctatgccg ccgtcgcccc aagttttttg caggcgtttg
     1141 cgcaggctgt cggtaaagaa gtcgccggcg gtatgcgagt cgccgatttg caggatgcgg
     1201 aaggtctcgc cgctgccttg tgcgacggat tggagttttt tcatccaagg cgaggcggcg
     1261 gcgttgccgt agtcggtcag cagcccgttt gtatcggtgt agggggcgga cggggaaacg
     1321 gtaacggtgt cgagactgac ggaggcaaca ggtagggcgt ttgcctgggt tgcggcgaac
     1381 agggcggaaa atgcgatgag gtgtttgggg ttcatggctg tgtacttgat ggttgcgtac
     1441 ttggttcaaa aacgattttt tccattattt ttgccgccag cagtttttgt ccttcggcgg
     1501 taaagtgtat gccgtccttg ctgcggtagc ggacgggttt gccgttgacg ttgacggagt
     1561 ccgtgtagcg gtcttccccg ccgctcaggg tgtgcgcggt gggaatcagg atgattttgc
     1621 ctttcaaatg ttccgaaagc agtttgtcga ggtagcgcat ctgcccgtca agcttggctt
     1681 ttttcatgta ggggatgccg agccagacga cttggacgcg gtgcgtgtgt gcggcttcaa
     1741 ggatgcgctc gacgcgtttc aggtattctt gcgcccattc gtcggaagca aatttgaggt
     1801 agcgtttgcc gacgggaaaa tcccacgggt cgttcgggcc gaggaagacg gcgagtacgc
     1861 tgatttcggg atgtttttgc agggtttctt caatcgtttt cggccagtcg aagaatgagg
     1921 ggtaggacag ccccgtgctt tgtttgctga ggttgacgga ttcgatgccg tattgctgtt
     1981 tcaggctttt ttgcacgaag ggtgcaacgc cctgcatcag cgagtcgccg gcgaaaaaga
     2041 ctttgtcgcc gctgcggacg gcggcggctt cggtgtcttg tttccattct gtttcaccgg
     2101 tttgagggac ggcttcagac ggcatatctg ccgagccgcc gtcttgagcc gtcggcggcg
     2161 tttcgccgga caggaatgtt ttgatgccgt ctgaaaaggc gtaggcgttt tcttgcaacg
     2221 ccgcaccgct ccgccaccat ccgtaggcgg caagcggttc gagcggactg ttgcggtggt
     2281 aggtctgctg ccagtaggcg ttgatggggt tttggctgaa ccacacggca atcagggcag
     2341 acatcaatat ggaagtgaaa agggaaagaa agtgtttcat gtccaaaccc ttaaaaattg
     2401 gcataaataa agccgggaat ccccgagggg gcgaatacga taatcagcag caggacggcg
     2461 gaaacgggga taaaccacag ccacatcggg attttttcca aacctttgac cgcgccgtcg
     2521 aaagcgcgtt gcaggtaagg gtagagcagc atcacggatg caaacgaggc aagcagcagc
     2581 atatccgccc gttgcggcgc attccagccg ttggcattgg caaagagggc attgaaaact
     2641 gcgcctgcat cgtcggggtt tgcggtattg aagacgacga aggtcaggca gacgaaatgg
     2701 aaggtaacaa accacgagag cggcgcaagg tatttcagac ggcatagtgc gttgcgtccg
     2761 aaatagcggt cgcccgtgtt gagcagcacc agcgccgtgc cgtgcagcgc gccccaaatg
     2821 aggaagttcc agccgtagcc gtgccagatg cctgagagta ccattgccgc catcaggttg
     2881 agctgtgtcc gtaaaaagcc ttttttgctg ccgcccaagg ggatgtagat gtagtcgcgt
     2941 atccaggtgg aaaggctgat gtgccatttg tcccaaaatg cacggatatt cgcggcacga
     3001 agcggtgctg agaagttttt gggcagtcgg aagcccagca gcattgccat gccgataacc
     3061 aaatcggaat atccggaaaa gtccaggaag agttggaagg tatagccgta cacgccgccc
     3121 aatacgcccc agccgtcgaa ttgggcgggg ttttcaaata cgggcgatac ccagttttcc
     3181 gccagcatcc ccgccagcca ccattttttg gcaataccca gcagaatcag ggaaacggcg
     3241 agtgcgggac ggacgggcga acgcggttgg cgggtacgga tttgcgccaa tgcgcccgcc
     3301 tgttcgccgt ccgcgctttt gaatgcggcg gcgcggataa tcgggccgga ggtaacggtg
     3361 gggaaaaaac tcaggtgcag cagcagttcg tgccactcga aacgcgcggc gtgcggcgca
     3421 cggaagcagt aaaccaaata ggcgattgac tggaaggtgt aatacgaaag ccccagcggc
     3481 atcaggatgt cgattgcgcc gccttgtccg gcatattgtg caatcagcgg gcggaaaaag
     3541 tcgaaatatt tgaaaaagcc caagacggtc agcgagacgg caatgccgca ccccagccaa
     3601 aagcggcgcg tattttcgcg atcggaacgg agcagttcgc ccaaaaggta cacgcaggag
     3661 gaataaagga cgacgattgc cgcaaatacg gggccgatat ggtagagcca gcccataccg
     3721 gcagccaaaa gcagcaggtt ttggacggac gggtatttcg ccaagcccca gtaaatcggc
     3781 aggaaggcga gaaagaacag tgcgaactcg acagacagca gcggcataaa ccatccttgg
     3841 aataagaaaa taggcgttaa aacacggcaa agcccgttat tttaagcagg ggcggatatt
     3901 tcttcaatca aaatgccgtc tgaagggcgg gaggggcttc agacggcatc gcgcggtcag
     3961 gtttggatat ggcggttcaa gttcaggcac agccacagcc cggccgccgc cgcaagcata
     4021 ccggcgatgc cgatacggga aatgccgaca tattgcgtta cccaatgtcc caacagtgcg
     4081 ccgctgccga taccgacgtt
//
LOCUS       DAWKOG010000167         4095 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.167, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000167 DAWKOG010000000
VERSION     DAWKOG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.167"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(202..1098)
                     /gene="argB"
                     /locus_tag="WJK66_001868"
     CDS             complement(202..1098)
                     /gene="argB"
                     /locus_tag="WJK66_001868"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEZ7579524.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRMPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1268..2398
                     /locus_tag="WJK66_001869"
     CDS             1268..2398
                     /locus_tag="WJK66_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase family protein"
                     /protein_id="HEZ7579525.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFDNAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDHQDATARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVREKMLETGVDLFLDIHGDEGLPFIFVAGTEGV
                     PNYNPRISALETQFKTALLNASPDFQDEYGYEKDAPGQANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNIIGDLR"
     gene            2469..3320
                     /locus_tag="WJK66_001870"
     CDS             2469..3320
                     /locus_tag="WJK66_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEZ7579526.1"
                     /translation="MIIHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVIFGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDAEAAAHNPLWSGWLQQYSMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3403..4065)
                     /locus_tag="WJK66_001871"
     CDS             complement(3403..4065)
                     /locus_tag="WJK66_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HEZ7579527.1"
                     /translation="MDSRKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAVDDEAFEELFYPIMALGGIYDEEENGTIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
BASE COUNT          908 a         1193 c          989 g         1005 t
ORIGIN      
        1 aaaatcaaag ccggagaatt tatcccaaac aaccggattt caaaaaaacc agattcccgc
       61 cttcgcggga atgacggatt ttaggtttct gttttggttt ctgttttcgc gggaataaca
      121 acttagaaat tgcccgaaac cccaaaaaca gaaaccggac aaacgggttt tccgccaaag
      181 ccggcatttt ccgactttgc ttcaggcatc ttccccaccg cccaaaatca tcgaaccgat
      241 accggcatcg gtaaagattt ccagcaaaag cgcgttgggc atcctgccgt cgatgatgtg
      301 cgtggctttc acgccgttga cggcggcttc gaccgcggaa gcgattttcg gcagcatacc
      361 gccatacagc gtgccgtcgg caatcagttc atcaatccgt ttcggcgtga gtttggtcag
      421 cagattgccc gttttgtcca tcacaccggc gatattcgtc atcatcaaga gtttttcagc
      481 gttcaattct tccgccaatt tgcctgctac caaatcggcg ttgatgttga acgcttcgcc
      541 tttttcacct acgccgacgg gggcgacgac gggaatgcag ccacgttcta tcagcccttt
      601 aaccaaaccg gtatcgatgc tttccaccgt accgacctgt ccgatgtcca cgccattctg
      661 ttcgggcgta tcgatcaaaa gtttcttcgc cttaatgaaa tggtcgtcgc gtccgcttac
      721 gccgaccgcg tgtccgccat atgtgttaat catcgacacg atttctttat tgacatgccc
      781 gcccaacacc atttcgacaa tatccatcgc ctctttgtcg gtaacgcgca ttccttggac
      841 aaactcaccc tttttgccga ctttttcaag catcgcattg atctgcggcc cgccgccgtg
      901 aacgatgacg ggatgaatgc cgaccagctt cagcagcacg acatcgcggg caaacccttc
      961 tttcaaggca ggttcggtca tcgcgttgcc gccgtatttg atgacggcga ccgaaccgga
     1021 aaaccggcgg atgtaaggca gcgcttcggc aaggatacgc gccttgtcgg cggcggaaat
     1081 aatgttttca gactccataa cggctcccta ttgttgacaa tttcggctta tgttaacgcc
     1141 aaacgccgtc tgaaacaatc ttcagacggc atccggcaga ctttcgggca ggttgaagcc
     1201 ttgcgatacg ggcggtaaaa tcgcggttta tccggttcga gaaaccggcg acagcaagga
     1261 ctttcccatg attaaaatca gcacccgctt cgatgccggc tcggtcgtcg tcaaagacct
     1321 gaccgatcct tccaacatcc gcctcgccct gcgtcccgac aacgcctccg attttgcaca
     1381 atggttctac ttccggctgc aaggcgcggc atatcaaaac tgcatcatgc attttgacaa
     1441 tgcggcagaa gccgcctacc cgaaaggctg ggaaggctat caggcgtgtg cctcatacga
     1501 ccgccgcaac tggttccgcg taccgacttc ctacgaaaac ggcgtactga ccgtcaatca
     1561 tacgccgctg tccaacagcg tgtattacgc ctatttcgaa ccttactccg aagaacagca
     1621 tttaaacctc ctcggcgacg cgcaaggcag cggcctgtgc cgcatcgacg acttgggcag
     1681 caccgtgcaa gggcgcgaca tcaatctgct gaccataggc aatcaggtcg aaagcgattt
     1741 gaaaatctgg attaccgccc gccagcatcc aggcgaaacc atggcggaat ggtttgtcga
     1801 aggactgctc ggcaggctgc tcgaccatca ggatgcaacc gcgcgcaccc tgctcgatcg
     1861 cgccacgttc tatatcgtcc ccaacatgaa ccccgacggt tcggcactgg gcaaccttcg
     1921 caccaacgcc gccggtgcga acctcaaccg cgaatgggaa aatccgactt tggaaaaaag
     1981 ccccgaagtg ttcttcgtgc gcgaaaaaat gctggaaacc ggcgtggatt tgttcttgga
     2041 catccacggc gacgaagggc tgccctttat ctttgttgca ggaactgaag gcgtgccaaa
     2101 ctacaatccg cgcatttccg cgttggaaac acagttcaaa accgcccttt tgaacgccag
     2161 ccccgatttc caagacgaat acggttacga aaaagatgcg cccggtcagg caaacatgac
     2221 cttggcaaca aactgggtcg gcaaccgttt caactgcctt gcctatacgt tggaaatgcc
     2281 ctttaaagac aatgccaacc tgcccgacga cgatttcgga tggaacggtc aacggtcgtt
     2341 gcgcttgggc gaagccgcgt tgtccgccat cctcaacatc atcggcgatt tgcgctgaaa
     2401 cccttattgc agaaatgccg tctgaagcat catgcctttc agacggcatt tgaaaacgaa
     2461 agcctgttat gattatccat ccccaattcg atcccgtcct catcagtatc ggcccgcttg
     2521 ccgtccgctg gtatgcccta agctacatcc tcggatttat tctttttacc tttctcggca
     2581 gaaggcgcat cgcgcaaggc ttgtccgttt ttaccaaaga atcgcttgac gacttcctca
     2641 cttggggcat tttgggcgta atttttggtg ggcgtttggg ttacgtcctg ttttacaagt
     2701 tttccgacta cctcgcccat ccgcttgata ttttcaaggt atgggaaggc ggaatgtcgt
     2761 tccacggcgg ctttttgggt gtagttattg ccatatggtt gttcggccgc aaacacggca
     2821 tcggcttcct caaactgatg gacacggtcg caccgctcgt tccgctgggt ctcgcttcgg
     2881 gacgtatcgg caacttcatc aacggcgaac tttggggacg cgttaccgac atcaacgcat
     2941 tttgggcaat gggcttcccg caggcgcgtt acgaagatgc cgaagccgcc gcgcacaatc
     3001 ccctttggtc aggttggttg caacaatata gtatgctgcc ccgccatccc tcgcagcttt
     3061 atcagtttgc acttgaaggc atctgcctgt tcgccgtcgt ttggctgttc tctaaaaaac
     3121 agcggccgac cggacaagtc gcctcgctct tcctcggcgg ctacggcata ttccgcttca
     3181 ttgccgaatt cgcacgccaa cccgacgact atctcgggct gctgaccttg gggctgtcga
     3241 tggggcaatg gttgagcgtc ccgatgattg ttttgggtat cgtcggcttt gtccggttcg
     3301 gcatgaaaaa acagcactga ccgcctgtga aaaataccgt ctgaaacatg gaaatccggt
     3361 ttcagacggc atttttacgt ttggttcatg gaaacaaacg cttcaattct taccgcaaca
     3421 cgccttgtat tttctgccgc tgccgcacgg acagggatcg ttcctgcctg ttttttcgcc
     3481 ttccctgcgg acggtttgcg gtttgttgat gactgcctgc cagtagcggt agatatccgc
     3541 caatgcataa ggcaactcgg attccagttc cgccagctcg ccttctgtga attgcagacg
     3601 gatagtgccg ttttcctctt cgtcgtaaat accccccaat gccatgatgg gataaaacaa
     3661 ctcttcaaac gcttcatcat cgacggcttc aaaccaatcg gtcggcacaa tatccaaacc
     3721 gtaaagataa gcattgcacc atgtgtaaaa atcgctgccg ccgtcttcgt tttcatacag
     3781 ccacaaatcg ggcagttttt tatccgacat cgcggcggtt gtttccatcg ccattgccaa
     3841 aaccagccgt tcgatttcgg aacgttcggc ggcggtaaat tgcgattcgt cgcccaacac
     3901 ttcgggcagc cagtcgagcg gtgtcaattt gtccggcccg ctcaacagcg ccgtcataaa
     3961 accttgaacc tcgtcgcaac gcatcgtgtt gccttgttcg cttttggcat ccaacaattc
     4021 gctcaaccgc cgtttggatg cttcggtaaa ttttcgggaa tccatcattt tccttttcaa
     4081 atgggttttg cgccc
//
LOCUS       DAWKOG010000168         3973 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.168, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000168 DAWKOG010000000
VERSION     DAWKOG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.168"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            215..1732
                     /locus_tag="WJK66_001872"
     CDS             215..1732
                     /locus_tag="WJK66_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ7579528.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALLQVQRSVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAAKLDLPAPVLENVGRFRKKTEGLTGWRFSGGISPAVNQNANNAAPQYCRQNGGR
                     QICSVSRAERAAGLNYEIEAEKLTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLPPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGVAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(1793..3574)
                     /locus_tag="WJK66_001873"
     CDS             complement(1793..3574)
                     /locus_tag="WJK66_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component I
                     family protein/aminotransferase class IV"
                     /protein_id="HEZ7579529.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFGPEELDALDRALQKGWQ
                     KGLHSVLFADYEFGLPLTGVESERGGNLALHWFADCADTDAASWLARHSDDLPSGIST
                     PQSSVSETDYLDRIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDAEGQSAWTLCFSPELFLNIASDGTISTEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQIGKVCVPEPFKVSRFGSVWQMTSTIQAQALPNTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLETEARGLYTGSIGYLKPCAGGLGFEGAFNV
                     VIRTLSLTPLSDGIYQGVYGVGSGIVIDSDPAAEYRECGWKARFLNELRPDFGIFETL
                     RAENGRCTLLDRHLCRLKTSAQALNLPLPDGCENQIKQYIADLPDGAFRVKALLASDG
                     ISLSRAVLNRLTDKQRVIISPTILPAQNYLRRFKTTCRTVFDQAWQTAETQGAFDSLF
                     FNSDGILLEGGRSNVFVKHRGQWLTPSLDLDILNGIMRQAVLDEPQKYLQTNQVIETH
                     ITQKTLQEAEEIRLSNALRGVFAAALA"
BASE COUNT          825 a          893 c         1295 g          960 t
ORIGIN      
        1 ttccgtacta tctgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac
       61 tataaatgtg aaatccgccc tttgaaaatc gggcggcggt tttttgcctg cccgctttca
      121 gacggcaaag ccggttttca cgggtttctg cctgtttttc ggatggtttg acgtgcttcg
      181 gcggcgtgtt tgccggaaag gtaaatgaca gggtatgttg tatttcagat acggcttttt
      241 ggttgtttgg tgtgcggcag gtgtttctgc cgcctatggg gcggatgcgc ccgcgatttt
      301 ggatgacaag gcattgttgc aggtgcagcg gtcggtgtcg gataagtggg cggaatcgga
      361 ttggaaagtt gaaaatgatg ccccgcgcgt ggttgacggg gattttttgt tggcgcatcc
      421 gaaaatgttg gaacatagtt tgcgcgacgc gctcaacggc aatcaggcgg atttaatcgc
      481 ttcgttggcg gatttgtatg ccaagctgcc ggattatgac gcggttttgt acggcagggc
      541 gcgggctttg ctggcgaaat tggcgggaag gccggcggag gcggtggcgc ggtatcggga
      601 actgcacggg gaaaatgcgg cagacgagcg gattttgctg gatttggcgg cggcggagtt
      661 tgacgatttc cgactgaagt cggcagaaag gcattttgcg gaggcggcaa aattggattt
      721 gccggcaccg gttttggaaa atgtggggcg ttttcggaaa aaaacggagg ggctgacggg
      781 ctggcgtttt tcgggcggca tcagtccggc ggtcaatcaa aacgccaata atgccgcgcc
      841 gcagtattgc cggcaaaacg gaggccggca gatatgcagt gtcagccggg cggagcgggc
      901 ggcagggttg aattatgaaa tcgaggcgga aaaactgacg gcgttggcag ataatcatta
      961 tttgttgttc cgttccaata tcggcggcac gagctattat ttcagtaaaa aatcagctta
     1021 tgacgacggg ttcggcaggg cgtatttggg ttggcagtat aaaaatgcac ggcagacggc
     1081 ggggattttg ccgttttatc aggtgcagtt gtcgggcagc gacggctttg atgcgaaaac
     1141 aaaacgggta aacaaccgcc gcctgccgcc gtatatgctg gcgcacggag tcggcgtgca
     1201 gctgtcccat acttaccgac caaacccggg atggcaattt tcggtcgcgc tggaacatta
     1261 ccgccaacgc taccgcgaac aggatagggc ggaatacaat aacggcaggc aggacgggtt
     1321 ttatgtttcg tcggcaaaac gtttgggcga atcggcaact gtgttcggcg gctggcagtt
     1381 tgtgcggttt gtgccgaaac gcgaaacggt gggcggcgcg gtcaataatg ccgcctaccg
     1441 gcgcaacggt gtttatgccg gttgggcgca ggagtggcgg cagttgggcg gtttgaacag
     1501 tcgggtttcc gcgtcttatg cccgccgcaa ctataagggc gttgcggctt tctcgacaga
     1561 ggcgcaacgc aaccgcgaat ggaatgtctc gctggctttg agccacgaca agttgtcgta
     1621 taaaggtatc gtgcccgcgt tgaattatcg tttcggcagg acggaaagta atgtgccgta
     1681 tgcgaaacgc cgcaacagcg aggtgtttgt gtcggcggat tggcggtttt gaatggtgga
     1741 ataatgccgt ccgaactttg tggaaacagg ttcggacggc atttttgcgc gttcaggcaa
     1801 gggcggcggc aaatacgccg cgcaaggcgt tggagaggcg gatttcttcg gcttcttgca
     1861 gtgttttttg tgtgatgtgt gtttcgatta cttgatttgt ttgcaaatat ttttgcggtt
     1921 cgtccaatac ggcttggcgc attatgccgt ttaaaatgtc caaatctaaa gagggcgtaa
     1981 gccattgtcc gcgatgtttg acgaacacgt tgcttctgcc gccttcgagc aggatgccgt
     2041 ctgaattgaa aaacaggctg tcgaacgcgc cttgtgtttc ggcggtttgc cacgcttggt
     2101 cgaagacggt gcggcaggtg gttttgaagc ggcgcaggta gttttgtgcg ggcaggatgg
     2161 tcggcgaaat gatgacgcgt tgtttgtcgg tcagacggtt taaaacggcg cgggacaggc
     2221 tgatgccgtc tgaagcgagc agggctttga cgcggaacgc gccatcgggc aagtcggcaa
     2281 tgtattgttt gatttgattt tcgcagccgt cgggcagggg caggttgagg gcttgggcgg
     2341 aggttttcag acggcatagg tggcggtcga gcagggtgca gcgtccgttt tccgcgcgca
     2401 gggtttcaaa aatgccgaag tcggggcgca attcgttgag gaaacgggct ttccagccgc
     2461 attcgcgata ttcggcggcg gggtcgctgt cgatgacgat gccggaaccg acaccgtaca
     2521 cgccttgata aatgccgtct gaaagcggcg tgagcgataa ggtgcggatg acgacgttga
     2581 acgcgccttc aaaccccaag ccgcccgcgc aaggtttcag gtagccgatg ctgcccgtat
     2641 aaagcccacg cgcttcggtt tcgagcgatt cgataatctg catactcatt tttttgggcg
     2701 cgccggtgat gctgccgcag gggaaggcgg cgcggaggat gtcggcgaac gaggtgtttg
     2761 gcagggcttg ggcttggatg gtgctggtca tctgccaaac gctgccgaaa cgcgacactt
     2821 taaacggctc gggtacgcat actttgccga tttgggcgat tttgccgagg tcgttgcgca
     2881 gcaaatcgac aatcatcacg ttttcggcgc ggtttttcgg gtcggcttgc aattcggcgg
     2941 cgcggcgttc gtcttgtccg tcgcccaaaa tcggcgcggt gcctttcatc ggttcggtgc
     3001 tgatggtgcc gtctgaagcg atgttgagga agagttcggg cgagaaacac agcgtccacg
     3061 cggattgccc ctccgcatcg ggcaggtggg acaagacggc ataggggacg ggctggcgca
     3121 ggcggcggta gaggctgacg ggattgccgt aggcttgcag gtgcaggcga gtggtgtagt
     3181 tgatttgata ggtgtcgccg cgccggatgg cttcgtggat ttggcggatg cggtcgaggt
     3241 aatcggtttc ggatacggag gattgcggcg tggaaatgcc ggagggaagg tcgtctgagt
     3301 gtcgggcaag ccagcttgcg gcatcggtgt cggcgcagtc ggcaaaccag tgcagggcaa
     3361 ggttgccgcc gcgctcggac tcaacccccg tcagcggcaa accgaattcg tagtctgcaa
     3421 acaacaccga atgcaaccct ttttgccagc ccttttgcag cgcgcggtcc aaagcatcga
     3481 gttcttcggg accgaaaaaa cggctttcta catgattttg atagcgtttt gcgcggccgc
     3541 ttacggcatc gtcaaacagg gcgaaatagg gcatggcaat tcggggcaaa tgttttgatt
     3601 atacgctctt tttacacata ttttcagacg attcggcaaa tatcggcaaa atgtaatttt
     3661 atgtagagaa agtggggggc gagggtgtaa aattgtgaaa acaaattccg atttccaacc
     3721 tgaaatacaa taaggagacc tttatgacag accaccagct gcaaccgttt gaaaacgtag
     3781 aattaggcga aaagcaagac cagctccaag tatagtggat taacaaaaac cagtacagcg
     3841 ttgcctcgct ttagctcaaa gagaacgatt ctctcaggtg ctgaagcacc aagtgaatcg
     3901 gttccgtact atctgtactg tcttcggctt cgttgtcttg tcctgatttt tgttaatccg
     3961 ctataaagac cgt
//
LOCUS       DAWKOG010000169         3931 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.169, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000169 DAWKOG010000000
VERSION     DAWKOG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.169"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..332)
                     /locus_tag="WJK66_001874"
     CDS             complement(<1..332)
                     /locus_tag="WJK66_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ7579530.1"
                     /translation="MFSLYREDSLISRLNRDGYLTSPSADFLELLSLCRDIHALTDGA
                     FDPTVQALWKLYADYFAAHPDAETPPPENSVKEALKRVGFGKVSFDDREIRFTEKGMG
                     LSLNGIAQG"
     gene            complement(313..594)
                     /locus_tag="WJK66_001875"
     CDS             complement(313..594)
                     /locus_tag="WJK66_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nosX protein required for nitrous oxide
                     reduction"
                     /protein_id="HEZ7579531.1"
                     /translation="MGRHFGRRRFLTVAAVAAAGAAVSFLPNPFAADDEKRNKDEKRN
                     ENVFFWKGVALGSGAELRLFGVDDRQAADLVNKVLAEVARLEKIVQPLP"
     gene            complement(603..1397)
                     /locus_tag="WJK66_001876"
     CDS             complement(603..1397)
                     /locus_tag="WJK66_001876"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEZ7579532.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFIHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFAFKFEDFELEGYDPHPHIKAAVSV"
     gene            1781..3115
                     /gene="gdhA"
                     /locus_tag="WJK66_001877"
     CDS             1781..3115
                     /gene="gdhA"
                     /locus_tag="WJK66_001877"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEZ7579533.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
     gene            3257..3631
                     /locus_tag="WJK66_001878"
     CDS             3257..3631
                     /locus_tag="WJK66_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579534.1"
                     /translation="MIMDQYYMELKNKLSNRPILLDNTNDFLFVLVNTVKAMIENTDK
                     SQLSELDKILDGVTSQELKLAYDFCQGKFGQAGFSYRRHPNYFYLSSLIATFPEFELS
                     KADRDYLKGIINFDNYLLYELD"
     gene            3675..>3931
                     /locus_tag="WJK66_001879"
     CDS             3675..>3931
                     /locus_tag="WJK66_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone acetyltransferase"
                     /protein_id="HEZ7579535.1"
                     /translation="MIELKLVDESSFQAVLDLKISEADERARFVAPNVRSLADAWLYR
                     ENEDVFPRAIYWDKQVVGFLLLEIDKDEAEYFIWRIMIGQQ"
BASE COUNT         1066 a         1017 c          927 g          921 t
ORIGIN      
        1 ccttgcgcga tgccgttcaa ggacaaaccc ataccctttt cggtaaaacg gatttcacgg
       61 tcgtcaaacg agactttgcc gaagccgacc cgcttcaggg cttccttcac gctattctcc
      121 ggcggcggtg tttccgcatc aggatgggcg gcaaaataat cggcatacag tttccacaac
      181 gcctgcactg tcggatcgaa cgcgccgtcc gtcagcgcgt gaatatcgcg gcacaggctc
      241 aacagttcca aaaaatccgc cgacggcgaa gtcagataac cgtccctgtt caggcggctg
      301 atcaggctgt cttcacggta aaggctgaac aattttttcc aaacgcgcca cttccgccaa
      361 aaccttgttg accaaatccg ccgcctgtct gtcgtccaca ccgaacaggc gcagctccgc
      421 gccggaaccc agcgcgacac ctttccagaa aaacacattt tcattgcgtt tttcatcttt
      481 attgcgtttt tcatcatcgg cggcaaaagg attcggcagg aaagaaaccg ccgcgcccgc
      541 cgccgcaacg gcggcaactg tcaaaaaacg cctgcgcccg aaatgcctgc ccataccgcc
      601 tcctaaaccg acactgccgc cttgatgtgc ggatgcggat cgtagccttc caactcaaaa
      661 tcttcaaatt tgaaggcaaa caaatctttg acttcaggat tgattttcat cacaggcaag
      721 gcgcgcggtt cgcgttccaa ctgcaatgcg gcctgctcga aatggttgcg gtacaaatgc
      781 gcgtcgccaa acgtatggat aaactcaccg gcttccaatc cgcacacttg cgccatcatc
      841 atggtcaaca atgcgtagct ggcgatgttg aacggtacgc caaggaaaat atccgcacta
      901 cgctggtaaa gctggcagga cagtttgccg tcggcaacgt aaaactgaaa cagcgcgtgg
      961 caaggcggca aggccatctc atcgaccaaa gccggattcc acgccgatac aatcaggcgg
     1021 cgcgagtcgg ggtttttctt aatttgttcc accacattgg cgatttggtc gatatggcgg
     1081 ccgtcgggcg cgggccagtt gcgccactgg tagccgtaaa ccgggcccaa gtcgccgttt
     1141 tcgtccgccc actcgtccca aatggaaaca ttgttgtcct tcaggtattt gatattggta
     1201 tcgcctttga gaaaccaaag cagctcgtgg ataatcgaac gcagatgcag ctttttggtc
     1261 gtcagcagcg gaaaaccttt gcccaagtca aaacgcatct gataaccgaa tacggagcgc
     1321 gtgcccgtac cggtgcggtc tgatttgtcc gtaccgttgt cgaggacgtg gcgcatcaag
     1381 tccaaatagg ctttcatagc agtctttcat caaattaaac ggcgcatatt gtaacatttc
     1441 cggataatgc ccaaaacacg gatacaggca ggcaggattg ttggcaattt cagtcctttt
     1501 ccacagtaaa acccggtggg aaaacaaaat taccttgatt ggaatcaaaa aatctagttt
     1561 aattacttag aataaaattt caataatatt tttatttacg aaattaattt atgattattg
     1621 attaattatc ggcaacaaac atcaaacatc gaaaacatgg aaaaaataat gtcaacaatt
     1681 tttgccaaat cgggcttggc atcagaaaaa aataggttta tattcccacc tacaaatttg
     1741 ttttcccatt agtacactat caaccaaaag gagtatccga atgactgacc tgaacaccct
     1801 ttttgccaac ctcaaacaac gcaatcccaa tcaggagccg ttccatcagg cggttgaaga
     1861 agtcttcatg agtctcgatc cgtttttggc aaaaaatccg aaatacaccc agcaaagcct
     1921 gctggaacgc atcgtcgaac ccgaacgcgt cgtcatgttc cgcgtaacct ggcaggacga
     1981 taaagggcaa gtccaagtca accggggcta ccgcgtgcaa atgagttccg ccatcggtcc
     2041 ttacaaaggc ggcctgcgct tccatccgac cgtcgatttg ggcgtattga aattcctcgc
     2101 ttttgaacaa gtgttcaaaa acgccttgac caccctgcct atgggcggcg gcaaaggcgg
     2161 ttccgacttc gaccccaaag gcaaatccga tgccgaagta atgcgcttct gccaagcctt
     2221 tatgaccgaa ctctaccgcc acatcggcgc ggacaccgat gttccggccg gcgacatcgg
     2281 cgtaggcggg cgcgaaatcg gctacctgtt cggacaatac aaaaaaatcc gcaacgagtt
     2341 ttcttccgtc ctgaccggca aaggtttgga atggggcggc agcctcatcc gtcccgaagc
     2401 gaccggctac ggctgcgtct atttcgccca agcgatgctg caaacccgca acgatagttt
     2461 tgaaggcaaa cgcgtcctga tttccggttc cggcaacgtg gcgcaatacg ccgccgaaaa
     2521 agccatccaa ctgggcgcga aagtgctgac cgtttccgac tccaacggct tcgtcctctt
     2581 ccccgacagc ggtatgaccg aagcgcaact cgccgccttg atcgaattga aagaagtccg
     2641 ccgcgaacgc gttgccacct acgccaaaga gcaaggtctg caatactttg aaaaccaaaa
     2701 accgtggggc gtcgccgccg aaatcgccct gccctgcgcg acccagaacg aattggacga
     2761 agaagccgcc aaaaccctgt tggcaaacgg ctgctacgtc gttgccgaag gtgcgaatat
     2821 gccgtcgact ttgggcgcgg tcgagcaatt tatcaaagcc ggcatcctct acgccccggg
     2881 aaaagcctcc aatgccggcg gcgtagcaac ttcaggtttg gaaatgagcc aaaacgccat
     2941 ccgcctgtct tggactcgtg aagaagtcga ccaacgcctg ttcggcatca tgcaaagcat
     3001 ccacgaatcc tgtctgaaat acggcaaagt cggcgacaca gtaaactacg tcaatggtgc
     3061 gaacattgcc ggtttcgtca aagttgccga tgcgatgctg gcgcaaggct tctaagcaaa
     3121 cgccgccgct ccgcaaacaa aatgccgtcc gaaccgcaaa tgctgttcag acggcatttc
     3181 attatccgcc cgttcgaatc gggtgatact atttgtatcc agaattgttg gatagtttgg
     3241 gagtggagag gaagaaatga ttatggacca atactatatg gagttaaaga ataagttatc
     3301 taacagacca atcctactgg ataatactaa tgattttctt tttgttttag taaatacagt
     3361 gaaagctatg atagaaaata ctgataaaag tcagctttca gagttagata aaattttaga
     3421 cggtgtcaca agtcaagaat tgaaacttgc ctatgatttt tgtcaaggga aattcggtca
     3481 agcaggtttt tcttatcgta ggcacccgaa ttatttttat ttgtccagtt taattgcgac
     3541 ttttccagaa tttgagttgt ctaaagcaga ccgagattac ctcaaaggaa tcataaattt
     3601 tgataactac cttttatatg agttagatta gtctttaatc acctcaaaac cctatttatt
     3661 caaaaggaga aaaaatgatt gagctgaaat tagtagatga gagcagtttt caggcagtgc
     3721 tggatttgaa aatatctgaa gctgatgaac gagcacgttt tgtggctcca aatgtacgct
     3781 ctttagctga cgcatggctc taccgggaaa atgaagatgt gtttccgagg gcaatctatt
     3841 gggataagca agtggttggc tttctcctgc tggaaataga caaggatgaa gcagaatact
     3901 ttatctggcg gataatgatt ggtcagcagt g
//
LOCUS       DAWKOG010000170         3859 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.170, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000170 DAWKOG010000000
VERSION     DAWKOG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.170"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            490..1446
                     /gene="thiL"
                     /locus_tag="WJK66_001880"
     CDS             490..1446
                     /gene="thiL"
                     /locus_tag="WJK66_001880"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEZ7579536.1"
                     /translation="MKEFDFIKRYLQTGTDNDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFANVKPEDLAWKVLAVNVSDMAAMGAIPRWVLLSAALPELDEVWLKRFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDADGRELELHSLGFDHFG"
     gene            1439..1924
                     /locus_tag="WJK66_001881"
     CDS             1439..1924
                     /locus_tag="WJK66_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEZ7579537.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMLGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WJK66_001882"
     CDS             complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WJK66_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEZ7579538.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIDNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKITGQLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGTKPKRIKYKPVTR"
BASE COUNT          781 a          940 c         1080 g         1058 t
ORIGIN      
        1 aaggcaacgc tgtactggtt taaatttaat ccactatatc ggttgaaact ctgattttaa
       61 ggcggtagga tgtgggtttg cccatagaaa gggaatcctt tctgtatcaa gccctgaaag
      121 ggataattca tacaaattca cgcctttccc cttcattggg aaatggatgg aatcgtgcca
      181 gatgtgtgcg gcactgtatg ccggatatgg ttttatcatc agcccttttc ggttgaaacc
      241 ttgccagttg cagcgattga gcctaatcgg tggcggaagt tgccgctttg cattcggggt
      301 ggcgtgcagt gcggtgcttt gatatgccgt ttgtgtgttg aaacagggtg gtcggtgcat
      361 acgggtacgg tatggccaaa gctaaaagtg aaatacgctg aaacactgaa tgagccgctt
      421 tattgtttgt acggcctttg ctgccttgct atgatttaaa ttggattcgc ccgccggata
      481 ttttgggata tgaaagaatt tgacttcatc aaacggtatt tgcaaacagg cacggataat
      541 gatgtcgtat tgggcatagg cgacgatgcg gcgattgtcc gcccgcgtga aggcttcgat
      601 ttgtgtttca gtgcggatat gcttttgaag gacaggcatt tttttgcaaa tgtcaaacct
      661 gaagacttgg cttggaaggt tttggccgtc aatgtttcgg atatggcggc gatgggtgcg
      721 ataccgcgtt gggtgttgct gagcgcggcg ttgcccgaat tggatgaggt atggctgaaa
      781 cggttttgcg gcagcttttt cggtttggca aaaaagtttg gcgtaacgtt aatcggcggc
      841 gatacgacca agggcgatat ggcgttcaat gtaaccatta tcggcgaatt gccgaagggt
      901 agggcgttgc ggcgtgatgc ggcggttgcg ggcgacgata tttgggtgtc ggggcgtgtc
      961 ggtatggcgg cggcggcttt gaactgccgt ctgaaacggt gtgtgttgcc ggatgatgtg
     1021 tttgccgaat gcgaacaaaa gctgctccgc cccgagccaa gggttgggct ggggcttgcg
     1081 ctgttgccgt ttgcccgggc ggcgcaggat gtttcagacg gcctcgcgca agatttgggg
     1141 catatcctga ccgcttccgg cgtgggggcg gaaatttggg ccgattcgct gccgtcttta
     1201 tccgtattga aagatatttt gccccgagcg caatggctgt cttatacttt ggcgggcggc
     1261 gacgattacg agctggtgtt taccgcgccg gaaagttgcc gcagccgcgt gcttgatgcg
     1321 gcagaacggt gcggcgtgcc ggtaacgcgc atcggcaaaa tcaacggagg atgccgtctg
     1381 aaggttttag atgccgacgg cagggaattg gaactacatt ctttaggatt cgatcatttt
     1441 ggctgatttt aaacctgact ttgcgtggct gttgaaacga ccgttgtgtt ttttggcttt
     1501 tggtttcggc agcgggctgg ctccgttcgc gccgggtaca tttggcactt tggcggcact
     1561 gcctttggca tttgtgctga ttttgctcgg catagacggg ctgctgctgg cttttttgtg
     1621 tatcgtgctg tttatgttgg gcatacgcat ttgcgcttat gcggaacgtg aaacgggtgt
     1681 cagcgaccat ggtgggattg tttgggacga gattgtcgcc atgctgtttg tgctggcgtt
     1741 tgtgccgttc aggtggacgt ggtggctggc ggcatttgtc ctattccgtc tgtttgacgc
     1801 gctcaaaccg tctcccgtcg gttggtttga caagaatctg cacggcggtt tgggcattat
     1861 ggcggacgat atggcggctg cggtgatgac tttgattgtc ttgaggattg caatgctgtt
     1921 ttaaacggtg ctgccttgta aaaatgccgt ctgaaagcct ttcagacggc attgtttcgg
     1981 aggttaacgc gttaccggtt tgtatttgat gcgtttcggt ttcgtgcctt cttcgccgag
     2041 tcggcgtttc ttgtcggctt cgtattcctg atagttgccg tcaaagaaca cccatttgga
     2101 gtcgccttcg caagccaaga tatgcgtagc aatacggtcg aggaaccagc ggtcgtgcga
     2161 aatcaccatc acgctgccgg caaattccag caatgcgtct tccaacgcgc gcagggtttc
     2221 cacgtcgagg tcgttggacg gttcgtccag cagcaacaca ttgccaccgc ccaacaaggt
     2281 ttttgccaag tgcaaacgtc cgcgttcgcc gccggaaagc tgccccgtga ttttgctttg
     2341 gtcgctgcct ttgaaattga agcgtcccaa atattggcgg gcggggattt caaactgccc
     2401 gacctgtaaa atatcgcgac cttcggcaat gttgtcgaac acggttttgt cgttttgcaa
     2461 accttcgcgg ctttggtcaa tcaagctcat tttcacggtt tgcccgattt tcacttcacc
     2521 ggaatcgggc tgctctttgc ccgcaatcat tttaaacagt gtcgatttac ccgcgccgtt
     2581 cggaccgatg atgccgacaa tcgcgcccgc aggcactttg aagctcaaat cgtcaatcag
     2641 cactttgtcg ccgaacgatt tggaaacatt cacaaattca atcacttcgt tacccaaacg
     2701 ctcggcgacg ggaatgaaga tttcctgcgt ttcattgcgt ttttggtatt cgtagttgct
     2761 catttcttca aaacgagcca aacgcgcttt ggacttggct tggcggcctt tggcattttg
     2821 gcgcacccat tccaattcct gcttcatcgc cttcacgcgc gcggcttcgg attttgcctc
     2881 gttttccaag cgtttttctt tctgctccag ccaagacgag taattgcctt tccacggaat
     2941 accatggccg cggtcgagtt ccaaaatcca ttcggcggcg ttgtcgagga agtagcggtc
     3001 gtgtgttacc gcaacgactg taccggggaa gcgcacgaga aattgctcca gccactcgac
     3061 agattccgcg tccaagtggt tggtcggctc gtccagcaaa agcatatcgg gtttgctcaa
     3121 caagagtttg cacaaggcaa cgcggcgttt ttcaccgccg gacaaattat cgattttggc
     3181 atcccattcc ggcaggcgca gcgcgtcggc ggcgatttcc aattcgtgtt ccgcaccgcc
     3241 gcccgtggac gaacctgccg caataatcgc ttccaagcgg ccctgctctt ctgccaacgc
     3301 gtcaaaatcc gcatcaggat tggcgtactc ggcatacact tcttccaaac gtttctgcgc
     3361 ggcagccact tcgcccaaac cgctttccac ttcctcgcgc acggtttttt caggatcaag
     3421 ctcaggctct tgcggcaggt agccgatttt aatgccgccc atcggcacgg cttcgccctc
     3481 aaattcctta tccacacccg ccataatccg cagcacggtg gacttgcccg cgccgttcaa
     3541 accgagcagg ccgattttcg cgccggggaa gaaagaaagg gaaatatctt taatgatggt
     3601 tttctgcggc ggcacaacct tgctcacgcg cagcatagaa tagacgtatt gttgggacat
     3661 ggttttctcg ttttcatcaa acaaatttca gacggccatt ttaaccgata atttgattta
     3721 agccagttta tccgcgaacc ggtattgcca aaatcgggca ggattcataa aatccgctta
     3781 tccctttgaa attatataga caaaaaaata ataatgatag gggatcgccg ccccggcaac
     3841 catttcggat tttccaaag
//
LOCUS       DAWKOG010000171         3838 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.171, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000171 DAWKOG010000000
VERSION     DAWKOG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.171"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..414)
                     /locus_tag="WJK66_001883"
     CDS             complement(<1..414)
                     /locus_tag="WJK66_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEZ7579539.1"
                     /translation="MTHISSPRNEFIRGIKESSPILIGLLPWALILGMQGGQKGMSWL
                     EMLLMTSMNFAGGSEFATVNLWAESLPILLIATVTFMINSRHILMGAALALHLKEIPL
                     KKAVPALFFMCDESWAMAFSEIQKRKAAGLPAFNMP"
     gene            562..1014
                     /gene="dut"
                     /locus_tag="WJK66_001884"
     CDS             562..1014
                     /gene="dut"
                     /locus_tag="WJK66_001884"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEZ7579540.1"
                     /translation="MNIEVEMKVLDERMADFIPTYATAGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPSYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFTVKPFERIAQMVIVPIVQARFKRVEEFVGSSRGEGGFGSTGLH"
     gene            1090..2277
                     /gene="dapC"
                     /locus_tag="WJK66_001885"
     CDS             1090..2277
                     /gene="dapC"
                     /locus_tag="WJK66_001885"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEZ7579541.1"
                     /translation="MNTLLKQLKPYPFARLHEAMQGISAPEGMEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTVDADNEILPVLGSREA
                     LFSFVQTVLNPVSDGIKPAIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWDDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAEDIVS
                     LYR"
     gene            2539..3318
                     /gene="yaaA"
                     /locus_tag="WJK66_001886"
     CDS             2539..3318
                     /gene="yaaA"
                     /locus_tag="WJK66_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEZ7579542.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESDILMQQLRELAPQ
                     QIAELMHVSDKIALLNAQRNAEWNTPFTPENAKQAVFMFNGDVYEGMDANTLNTDQIQ
                     YLQGRVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKKLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHNITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            3401..3491
                     /locus_tag="WJK66_001887"
     tRNA            3401..3491
                     /locus_tag="WJK66_001887"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3435..3437,aa:Ser,seq:tga)
     gene            3704..3793
                     /locus_tag="WJK66_001888"
     tRNA            3704..3793
                     /locus_tag="WJK66_001888"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3738..3740,aa:Ser,seq:cga)
BASE COUNT          991 a          900 c          958 g          989 t
ORIGIN      
        1 aggcatattg aatgcgggca aaccggctgc tttccgtttt tggatttcgg agaatgccat
       61 cgcccagctt tcatcacaca taaaaaacag tgcgggcacg gcttttttca gcggtatttc
      121 tttcaggtgc agggcaagtg ccgcccccat caggatatgc cgcgaattaa tcataaaggt
      181 tacggtggcg ataagcagta tcggcagaga ttccgcccac aggttgaccg tggcaaactc
      241 ggagccgccg gcgaagttca tactggtcat caacaacatt tccagccagc tcatgccttt
      301 ttgtccgccc tgcataccga gtattaatgc ccaaggcaga agtccgataa gtatgggcga
      361 actttctttg atgccgcgta taaattcgtt acggggcgag cttatgtgtg tcatattata
      421 atattgatat gtaagaaggt cgggcattat acacagacgg agtatttcaa aacaaaacct
      481 atttgctgtg tttcagtgta aacacggctt gtgtataatc caccatcttt agacagaccg
      541 accgtatgtg ggagcaaggc aatgaatatt gaagtagaaa tgaaagtgtt ggacgagagg
      601 atggcggatt ttatccctac ctatgcgaca gcgggttctg ccggtttgga cttgcgcgcc
      661 tgtttggatg aggaagtcgt cttgcagccg ggggaaacgt ttcttgtgcc gacaggtttg
      721 gcaatttatt tggccaatcc ttcatatgcc gccgttttgc tgccccgttc cggcttgggg
      781 cataaacacg gcattgtttt gggcaatttg gtcggcttga ttgactccga ttatcaaggg
      841 gaattgaagg tttcgttatg gaacagaagc agcgagccgt ttactgtcaa accgtttgag
      901 cgtatcgcgc agatggttat cgtgccaatc gtgcaggcgc gtttcaaacg tgtcgaggaa
      961 tttgtcggaa gcagccgtgg agaaggcggt ttcggcagta cgggtttgca ttgaatataa
     1021 aatgccgtct gaagggcacg tcaggttcag acggcatatc ttccgatata ccgataaccg
     1081 gagaaaatca tgaatacctt actcaagcag ctcaaaccct atccctttgc ccgactgcac
     1141 gaggcgatgc agggcatttc cgcgcctgaa ggcatggaag ccgtccccct gcacatcggc
     1201 gaaccgaaac atccgacacc gaaagtcatt acggatgcgc tgaccgcctc attgcacgag
     1261 ttggaaaaat atccgctgac tgcgggtctg cctgaactgc gtcaggcgtg tgcaaactgg
     1321 ttaaaacgcc gttacgatgg cttgacagtg gatgcggata atgaaattct gccggtttta
     1381 ggcagtaggg aggcgttgtt ttcttttgtt caaaccgtgt tgaaccctgt ttcagacggc
     1441 atcaaacccg caattgtcag cccgaatccc ttttatcaga tttacgaagg tgcgacactt
     1501 ttgggcggcg gtgaaatcca ttttgccaat tgccccgcgc cgtctttcaa ccccgattgg
     1561 cgtagtattt ccgaagaggt ttggaaacgc accaaactgg tgttcgtctg ctcgcccaac
     1621 aaccccagcg gcagcgtgct ggatttggac ggatggaaag aagtttttga tttgcaggac
     1681 aaatatggtt tcattattgc ctcggacgaa tgctattccg aaatctattt cgacggcaac
     1741 aagcctttgg gctgcctgca ggccgctgca cagttggggc gaagcaggca aaaactgctt
     1801 atgttcacca gtttgtccaa gcggtccaat gttccaggcc tgcgttccgg ttttgtcgcc
     1861 ggcgatgccg aactgcttaa aaattttctg ctttacagaa cctatcacgg cagcgcaatg
     1921 agcattcccg tgcagcgcgc aagcattgcc gcttgggatg acgaacagca cgttatcgac
     1981 aaccgccgcc tgtatcagga aaaatttgag cgcgttattc ccattttgca acaggtattt
     2041 gacgttaaat taccggatgc ctcgttttac atctggttga aagtccccga tggcgacgat
     2101 ttggcatttg cacgcaattt atggcaaaaa gccgctatcc aagtattgcc cggacgcttt
     2161 ttggcacggg ataccgaaca gggcaatccc ggggaaggtt atgtccgtat cgctttggtt
     2221 gccgatgtcg caacttgtgt caaagctgcg gaagacatag tttcactgta tcggtaagga
     2281 atcggaaatg ccgtctgaac ttttgttcag acggcatttt tcaatattct acggttgaat
     2341 ttgctgtaac ggtatttata gtggattaac aaaaatcagg acaaggcgac gaagccgaag
     2401 acagtacaga tagtacggca aggcgaggca acgctgtact ggtttaaatt taatccacta
     2461 tacttcactt ttaatcggct tgcccgcaaa cacgtttaaa cttaaaatcc ccgtgtttga
     2521 cacaataccg agcagattat gttttttgtc ctttcccctg cgaagaacct taatgaaaaa
     2581 gaccctgccc ctgtcagcga gtttacccaa cccgacctgc tggcagagtc cgacattcta
     2641 atgcagcagt tgcgcgagct tgcgccgcaa cagattgccg aactgatgca cgtttccgac
     2701 aaaattgccc tcttaaacgc gcagcgcaat gcagaatgga acacgccgtt tacgccggaa
     2761 aacgccaaac aggcggtctt tatgttcaac ggcgatgttt acgaaggtat ggatgcaaac
     2821 acattgaata ccgatcaaat acaatatctg caagggcgcg tacgcctgct gtccggcctg
     2881 tacggccttc tgcgtccact ggacctgata cagccttacc gtttggaaat ggggacggca
     2941 tttgccaatt tgcgcgggaa gaatttgtat gagttttggg gagacatcat taccaacctt
     3001 ttaaatgaca cgcttgccca agcaggcagc aatacgcttg tcaaccttgc ctcacaggaa
     3061 tatttcaagt ccgtcaacac gaaaaaactt cgggcgcggc tgattacccc gatatttaaa
     3121 gacgaaaaaa acggcaaata taaaatcatc agtttctatg ccaagcgcgc gcgaggatta
     3181 atggtgcgct atgcggcaga acacaatatt accgatcctg aaatgctgaa aaattttaat
     3241 tacgaaggct acgcatttaa tgacgcggct tcaaatgaaa gcgaatgggt gtttatgcgt
     3301 tcggaacaaa taaagtgaaa acaataaatt aagtatttcc caaaaaaagt gcttggcaaa
     3361 atgtataaac ttcattatta ttcctactct tcaagaagac ggaagcgtgg cagagcggtt
     3421 taatgcaacg gtcttgaaaa ccgtcgaggg ttgatagccc tccgtgagtt cgaatctcac
     3481 cgcttccgcc aatccttgag gtcaaaaacc aaataaaaat gaagtagcat aaagcatcgg
     3541 catttttatt tggttttact tcatcgtgtt tccttttcgg ttgaacccca ccctttaggg
     3601 cggtagaatc agactttatt tgggaggggt gtaacccttt ctgaatcagg gtaacacata
     3661 gggcggtgct ttatgtgtcg tcctgtgtgt tgaaacatct ttcggaaacg tggcagagag
     3721 gctgaatgca gcggactcga aatccgctga gggtgcaaat cctccgtggg ttcgaatccc
     3781 accgtttccg ccacaaaaca aaaccgccct gattcggggc ggttcttttt tgttcaag
//
LOCUS       DAWKOG010000172         3778 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.172, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000172 DAWKOG010000000
VERSION     DAWKOG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.172"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            505..3618
                     /locus_tag="WJK66_001889"
     CDS             505..3618
                     /locus_tag="WJK66_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ7579543.1"
                     /translation="MNKTLKRQVFRHTALYAAILMFSHTGGGGAMAQTHKYAIIMNER
                     NQLEVKGNGQYSTIKDKDRERKFIYNKDRGGGGSVFFDNTDTLVSQQRGTAVFGTATY
                     LPPYGKVSGFDADGLQKRNNAVDWIHTTQAGLAGYAYTDVICRSNQCPQLVYETKFSF
                     DGIGLAKNAGSLDRHPDPSRENSPIYKLKDHPWLGVSFNLGSENTVKDGKSFNKLISS
                     FSEGNNNQTIVSTTRGHSISLSDWKREHTAMAYYLNAKLHLLDKKGIEDIAQGKTVDL
                     GTLRPRVEATVRRGELLNFWATWKIEDKGNITVRLGLPEVKAGRCVNKANPNPNAKAP
                     SPALTAPALWFGPVKDGKAEMYSASVSTYPDSSSSRIYLQNLKRKTDPGKPGRHSLET
                     LTENDIKSREPNFTGRQTIIRLNGGVREIKLDRNNTEVVNFNGNDGNNDTFGIVKDLG
                     VEPDTSEWKKVLLPWTVRGFADDSKFKAFNKEENNDNKPKYSQKYRSRDNNKGERNLG
                     DIVNSPIVAVGEYLATSANDGMVHIFKQSGGDKRSYNLKLSYIPGTMPRKDIQNTEST
                     LAKELRTFAEKGYVGDRYGVDGGFVLRKVDNLNGQNRVFMFGAMGFGGRGAYALDLTK
                     ADGSDPTAVSLFDVKDNGNNGNNRVELGYTVGTPQIGKTHDGKYAAFLASGYATKEII
                     TSGDNKTALYVYDLEGNGTNNLIKKIEVPGGKGGLSSPTLVDKDLDGTVDIAYAGDRG
                     GNMYRFDLSSQDPQQWSVRTIFEGTKPITSAPAISQLKDKRVVIFGTGSDLSEEDVDN
                     MEEQYIYGIFDDDTATTGTVNFSGSGGGLLEQVLRRDNDNKTLFLTDYKRSDGSGNKG
                     WVVKLKDGQRVTVKPTVVLRTAFVTIHKYTGTDKCGAETAILGINTADGGKLTKKSAR
                     PIVPEANTAVAQYSGHKKGTNGKSIPIGCMQKSNEIVCPNGYVYDKPVNVRYLDEKKT
                     DGFSTTADGDAGGSGIDPAGKRSGKNNRCFSQKGVRTLLMNDLDSLDITGPTCGMKRI
                     SWREVFY"
BASE COUNT         1060 a          968 c          987 g          763 t
ORIGIN      
        1 aaccgctccg ccgtcattcc cgcgcaggcg ggaatccaga ccttgggata acagtaatat
       61 tcaaagatta taaaagaccc gtcattcccg cgcaggcggg aatccagacc ttagaacaac
      121 agtaatattc aaagattata aaagactcgt cattcccgcg caggcgggaa tccagactgt
      181 cgggcatctg cagcggtttg ctaaaaaacg ctttaccgtg atcagtgtgc aaagttaaaa
      241 tggggaggta agcttttcaa ccagcaattc ggcgggcgcg ggatcgggcg gtttgccgaa
      301 tcccgccgtt tgcggcgcgc ctgccgccga cggtatcccg cgaagcaaga tttaagggat
      361 aaaatatgtt ccaacacgca gggcggcaca taaggcgccg ccctgattcg gaagggcttg
      421 cacccctccc ggacaaagcc tgatcctgcc gccccgaagg acggatgccc gaagggcggg
      481 gggtttgacc gaaaaggaaa tacgatgaat aaaactttaa aaaggcaggt tttccgccat
      541 accgcgcttt atgccgccat cttgatgttt tcccataccg gcgggggggg ggcgatggcg
      601 caaacccata aatacgctat tatcatgaac gagcgaaacc agctcgaggt aaaggggaat
      661 gggcaatatt caacaataaa ggacaaagac agggaacgca aatttatcta taataaagac
      721 agagggggtg gaggctctgt ctttttcgac aataccgata cccttgtttc ccaacaaaga
      781 ggtactgccg tttttggcac agccacctac ctgccgccct acggcaaggt ttccggtttt
      841 gatgccgacg ggctgcaaaa gcgcaacaat gccgtcgatt ggattcatac cacccaggcc
      901 gggctggcag gctacgccta caccgacgtc atatgcagaa gcaaccaatg cccccaactt
      961 gtctatgaga ccaaattttc cttcgacggc atcggtttgg caaaaaatgc gggcagcctg
     1021 gataggcacc cggacccaag ccgcgaaaat tcgcccattt acaaattgaa agatcatcca
     1081 tggttgggcg tatctttcaa tttgggcagc gagaataccg tcaaagatgg caaatcattc
     1141 aacaaattga tatcttcttt tagtgaaggc aataataatc aaaccatcgt ctctacgaca
     1201 cgaggccact ctatttccct tagcgactgg aagcgcgaac ataccgccat ggcctattat
     1261 ctgaacgcca aactgcacct gctggacaaa aaagggattg aagatatcgc ccaaggcaaa
     1321 acagtggatt tgggcacctt gagaccgcgc gtcgaggcaa cggtaaggcg gggggagctg
     1381 ctaaattttt gggctacgtg gaagattgaa gataaaggga acattacagt ccgcctcggc
     1441 ctgccggaag tcaaagcagg ccgctgcgtc aacaaagcga accccaatcc caacgccaaa
     1501 gccccctccc ccgcactgac cgcccccgcg ctgtggttcg gacctgtgaa agatggtaag
     1561 gcggagatgt attccgcttc ggtttctacc taccccgaca gttcgagcag ccgcatctac
     1621 cttcaaaatc tgaaaagaaa aaccgacccc ggcaaacccg gccgccattc cctcgaaacc
     1681 ttgactgaga atgatattaa aagtcgagag ccgaatttca cagggcggca aaccatcatc
     1741 cgattgaatg gcggcgtacg tgagatcaaa ctggatagaa acaatactga ggtcgtcaat
     1801 tttaatggaa atgacggcaa caacgacact ttcggcattg ttaaggactt gggcgtcgaa
     1861 cctgatacca gcgagtggaa aaaagtattg ctgccttgga cggttcgggg ttttgctgat
     1921 gacagtaaat ttaaagcatt caacaaagaa gaaaacaacg acaacaagcc aaaatacagc
     1981 caaaaatacc gcagccgcga caacaacaag ggcgaacgca atttgggcga catcgtcaac
     2041 agccccatcg tggcggtcgg cgagtatttg gctacttccg ccaacgacgg gatggtgcat
     2101 atcttcaaac aaagcggcgg ggacaagcgc agctacaatc tgaagctcag ctacatccct
     2161 ggaacgatgc cgcgcaagga tattcaaaac accgaatcca cccttgccaa agagctgcgc
     2221 acctttgccg aaaaaggcta tgtgggcgac cgctacggcg tggacggcgg ctttgtcttg
     2281 cgcaaagttg ataacttaaa cgggcaaaac cgcgtgttta tgttcggcgc gatgggcttt
     2341 ggcggcagag gcgcgtatgc cttggatttg accaaagccg acggcagtga cccgaccgcc
     2401 gtttccctgt ttgatgtaaa agataacggc aataatggca ataatcgcgt ggaattaggc
     2461 tacaccgtcg gcacgccgca aatcggcaaa acccacgacg gcaaatacgc cgccttcctc
     2521 gcctccggtt atgcgactaa agaaattatt accagcggcg acaataaaac cgcgctgtat
     2581 gtgtatgatt tggaaggaaa cggtacgaat aatctgatta aaaaaatcga agtacccggc
     2641 ggcaagggcg ggctttcgtc ccccacgctg gtggataaag atttggacgg cacggtcgat
     2701 atcgcctatg ccggcgatcg cggcgggaat atgtaccgct ttgatttgag cagtcaagat
     2761 cctcaacaat ggtctgtacg cactattttt gaaggcacaa aaccgattac ttccgcgccc
     2821 gctatttccc aactgaaaga caaacgcgtg gtcatcttcg gcacgggcag cgatttgagt
     2881 gaagaggatg tggacaatat ggaagaacaa tatatttacg gtatcttcga cgacgatacg
     2941 gcgacgacgg gtactgtaaa cttcagcggt tcgggaggcg ggctgcttga gcaagtgctt
     3001 cgtagggata acgacaataa aaccttattc ctgaccgatt acaagcgatc cgacggatcg
     3061 ggcaataagg gctgggtagt gaaattgaag gacggacagc gcgttaccgt caaaccgacc
     3121 gtggtattgc gtaccgcctt tgtaaccatc cataaatata cgggtacgga caaatgcggc
     3181 gcggaaaccg ccattttggg catcaatacc gccgacggcg gcaagctgac caagaaaagc
     3241 gcgcgcccga ttgtgccgga agccaatacg gctgtcgcgc aatattccgg ccataagaaa
     3301 ggcaccaacg gcaaatccat ccctataggt tgtatgcaaa aaagcaatga aatcgtctgc
     3361 ccgaacggat atgtttacga caaaccggtt aatgtgcgtt atctggatga aaagaaaaca
     3421 gacggatttt caacaacggc agacggcgat gcgggcggca gcggtataga ccccgccggc
     3481 aagcgttccg gcaaaaacaa ccgctgcttc tcccaaaaag gggtgcgcac cctgctgatg
     3541 aacgatttgg acagcttgga cattaccggc ccgacgtgcg gtatgaaacg aatcagctgg
     3601 cgtgaagtct tctactgatt tgcacgcgaa aatgccgtcc gaaaggtttt cggacggcat
     3661 tttttgcgtt tttcgggagg ggcgggttcg taaaaggcgg gctatagggt aggcttcatc
     3721 tcgccaatct cactgaatcc atcaatttcc acaattcaat taaataccgt caaaccga
//
LOCUS       DAWKOG010000173         3751 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.173, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000173 DAWKOG010000000
VERSION     DAWKOG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.173"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(88..906)
                     /locus_tag="WJK66_001890"
     CDS             complement(88..906)
                     /locus_tag="WJK66_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ7579544.1"
                     /translation="MKGMDKLRYQQDFLNIRPIFTAGEQEYLTELSDRLPLSVLTDSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDRPDPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIGNPFDRAVYLHNNLAYLQYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FAPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDMDIFRNFSL"
     gene            complement(986..2236)
                     /locus_tag="WJK66_001891"
     CDS             complement(986..2236)
                     /locus_tag="WJK66_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ7579545.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAARIWPS
                     HTYTRLTLESTAALKYQHFALDNPSRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPTTVRLVIDLKQPTHAQVFSLSPVSGFKNRLVVDLYPQGMDPDDPMMALLNGS
                     LNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMIDPGHGGEDPGAVSSGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            complement(2233..2694)
                     /gene="tsaE"
                     /locus_tag="WJK66_001892"
     CDS             complement(2233..2694)
                     /gene="tsaE"
                     /locus_tag="WJK66_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEZ7579546.1"
                     /translation="MSDLPSISRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLERFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHDGDGRKCLLTAHTERGRESLPL"
     gene            2896..3708
                     /locus_tag="WJK66_001893"
     CDS             2896..3708
                     /locus_tag="WJK66_001893"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEZ7579547.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRNNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAGNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
BASE COUNT          836 a          899 c         1113 g          903 t
ORIGIN      
        1 cactataacc gaaggtaagg gaggggtgtg tccactgtgc cgccgcattt gccgcgtgcg
       61 gaagatacgg ataacaaccc cttggaatta aagggagaag ttgcggaaaa tatccatatc
      121 aggctgtggg ccgtacttat ttacggtatt ttcgtatgcc gaaataaaat atttcttgaa
      181 taaaccgtaa tcgccggttt cgtaataggc tacgacagcc tcggcatagg cgggataact
      241 gtcgggggcg aatacgcaag gaaaaaaacc ggagcgcatc agcgacaagg tcatacagtt
      301 tctggcagta cgtttattgc aatctttaaa atattgaaga tacgccaaat tattatggag
      361 ataaacggcg cggtcgaagg gatttcctat ttttggggct tcttgaagca accattttaa
      421 ttccgtatcc aaactttgcg ggttggataa aggcgtgtag tctgtcccgc ttatagtaac
      481 gctgtcgcgg cggactacgc cgccggagcc tttttccagt aagttttccg aaatcaggct
      541 gtgggtggtt tttaagaaat ccaaccagtc aaacggatcg gggcggtcca aaccggacag
      601 caggtgccgg taactttccc tcaggttgat taacattacg gcatccgaat ataacttccc
      661 gcctgcggtt tgcccgagtt taagcaatgc ctgcgtatcg tattgattat aagtgtttcc
      721 ttccaatttg gcagaactgt acacaaaatc gataccgatt tcttcaatat tacgcacgga
      781 gtccgttaaa acagataaag gaagccgatc ggacaattcg gtcaggtatt cttgttcgcc
      841 ggctgtaaaa atggggcgga tatttaaaaa atcctgttgg tatctcaatt tatccatacc
      901 tttcatcata aaaacgcggt ttggggcgga agtaggttta tgcctgcctt catccgacaa
      961 ctatattgaa acaccgcctt ttcaatcaac cccgcttcaa tacggatgta ttgatgtagc
     1021 gttggacgcc cgaggcaatg gattgggcgc actgccgacg gaaggattcg ctgcccagca
     1081 gcttctcttc ggcaggattg gacaggaagg cggtttcgac caggatagac ggcatatcgg
     1141 gcgcgcgcaa aacggcgaaa ttggcttcgt ccactctgcc tttgtgcaag tggttgagcc
     1201 tgcccaattc ttcaagcacc agtttgccga gtttgcggct gtcgcgcagc gtggcggttt
     1261 gggtcatatc gagcagggcg gtatcgacat tgcggttgcc gctggtcggt acgccgccga
     1321 ccgcgtcggc attattttgg gtttgttcca agaatttggc ggcagagctg gtcgcgcctt
     1381 tggtgtttaa catataaacc cccgtgccgc gcgcggaggg gctggtgaag gcatcggcgt
     1441 ggatggagac gaatacgtcc gcccgccgtg cgcgcccttt ggcgacacgc acgcccaaag
     1501 ggatgaacac gtcttcgttg cgcgtcataa agacgttgta acccaaggct tccaattgtt
     1561 ttttggtttc gcgggcgatg gagaggacga cgtgtttttc ctgcaaaccg cccgagctga
     1621 cggcgccggg gtcttcgccg ccgtgtccgg ggtcgatcat aatgacgggt ctgcgcccgt
     1681 ttttgccgcg ttcggtttgt gcgggagtgt tttgagcgag gtcggcttca ggggagccgc
     1741 gacgggtttt gttcaggctg ccgttaagca gcgccatcat cggatcgtcg ggatccatcc
     1801 cttgcggata gagatcgacg acgaggcggt tcttaaagcc gctgacagga gagagggaaa
     1861 agacttgtgc gtgggtgggc tgtttcaggt cgatgacgag gcggacggtg gtcggtgtgt
     1921 tctgacccgc gcgtatgctg cggataaagg ggtcgtccgc cataaccttc tgagacagtc
     1981 cgtgcaatac ggtattgatg ttcgcgtttt gtatatcgac gaccagcctg cttgggttgt
     2041 cgagcgcgaa gtgctggtat ttgagcgcgg cggtgctttc cagcgtcagg cgggtgtagg
     2101 tgtgcgatgg ccatatccgt gcggcagtga attgtggtgc gcgtaccgtt ttggcaacgg
     2161 cggatgcgat ggggcttagg gcgaacagtg tgccggcggt gcggcggatg atttgtcttc
     2221 gtgtcagttt ggtcatagcg gcaggctttc gcgtcctcgt tcggtatggg cggtcagcag
     2281 gcattttctg ccgtcgccgt cgtgtgtcaa tgttgcggtg atgtcggcag gcggcgtaaa
     2341 ttccccgccc tgttgcggcc attcgatcag gcagacgctg tttgcggcaa acagttcgtc
     2401 caatccggca tcttcccatt cttcggggaa cgagaagcgg tagaggtcga aatggtgcaa
     2461 ggtgaagcgt tccagcggat aagattcgac gatggcgtag gtcgggcttt tgactgcgcc
     2521 ctgatgaccc aatccgcgca ggatgccgcg tgtcagcgtg gttttgcccg cacccaaatc
     2581 cccttcgaga taaatgacca gcggtgcgtt taaacgggaa gaccacgccg cgcccaaatc
     2641 gagtgtggcg gcttcgtcgg caaggaatcg ggagatagag ggtaaatcag acatggaaac
     2701 ggtttgttgt aaggtctagg gtattatggg cagttttgca ggttttgcaa actttgcacc
     2761 cgaggggcgg atgcttcttg tccgagcatt ataacagcca aatccgcgtt ctgctttcag
     2821 acggcaacgg ctgtcaagaa aaagcggcgc gtgtacaata cgcggattgt atgtttagga
     2881 cggattggaa aaaggatgga aaatatcggc aggcagcgac ccatcggcgt ttttgactcg
     2941 ggaatcggcg gtttgaccaa tgtacgcgcg ctgatggagc ggctgccgat ggagaacatc
     3001 atttatttcg gcgacacggc gcgcgtgcct tacgggacga aatctaaggc taccatcgaa
     3061 aatttctcga tgcagattgt cgatttttta ttggaacacg atgttaaggc gatggttatc
     3121 gcgtgcaata cgattgcggc ggtggcgggg cagaaaatcc gtcaaaagac gggcaatatg
     3181 cctgtgttgg acgtgatttc cgccggcgcg aaagccgcgc tggcaacgac gcgcaacaat
     3241 aaaatcggca ttatcgccac caataccaca gttaacagca atgcttatgc gcgcgccatc
     3301 cacaggaaca accccgatac gctcgtccgc acgcaggccg cgccgctgct cgtccctttg
     3361 gtggaagagg gctggttgga acacgaagtt acccgcctga ccgtatgcga atacctcaaa
     3421 ccattgcttg cagacggcat cgatacgctg gtgttgggct gcacgcactt tcccttgctc
     3481 aagcccttaa tcggcaggga ggcgggcaat gtcgcgttgg ttgattctgc aattacaacg
     3541 gccgaagaaa ccgcacgcgt ccttgctcag gaaggattgc tcaataccga caacaacaat
     3601 cccgactacc gtttttacgt cagcgatatt cctttgaaat tcagaaccat cggcgagcgt
     3661 tttctgggca ggacgatgga gcagattgaa atggtgtctt tgggttaaaa cgatgacgga
     3721 aagctgcccg agattacaga aacctaaaat c
//
LOCUS       DAWKOG010000174         3748 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.174, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000174 DAWKOG010000000
VERSION     DAWKOG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.174"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            374..3577
                     /gene="nalP"
                     /locus_tag="WJK66_001894"
     CDS             374..3577
                     /gene="nalP"
                     /locus_tag="WJK66_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase autotransporter NalP"
                     /protein_id="HEZ7579548.1"
                     /translation="MRTTPTFPTKTFKPAAMALAVATTLSACLGGGGGGTSAPDFNAG
                     GTGIGSNSRATTAKSAAVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPNLHT
                     GDFPNPNDAYKNLINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGYNE
                     NYKNYTAYMRKEAPEDGGGKDIEASFDDEAVIETEAKPTDIRHVKEIGHIDLVSHIIG
                     GRSVDGRPAGGIAPDATLHIMNTNDGTKNEMMVAAIRNAWVKLGERGVRIVNNSFGTT
                     SRAGTADLFQIANSEEQYRQALLDYSGGDKTDEGIRLMQQSDYGNLSYHIRNKNMLFI
                     FSTGNDAQAQPNTYALLPFYEKDAQKGIITVAGVDRSGEKFNGSNHCGITAMWCLSAP
                     YEASVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSNDNLRTTLLTTAQDIGAV
                     GVDSKFGWGLLDAGKAMNGPASFPFGDFTADTKGTSDIAYSFRNDISGTGGLIKKGGS
                     QLQLHGNNTYTGKTVIEGGSLVLYGNNESDMRVETKGALIYNGAASGGSLNSDGIVYL
                     ADTDRSGANETVHIKGDLQLGGEGTLYTRLGKLLKVDGTAIIGGKLYMSARGKGAGYL
                     NRTGQRVPFLSAAKIGRDYSFFTNIETDGGLLASLDSVEKTAGSEGDTLSYYVRRGNA
                     ARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAAADRTDMPGI
                     RPYGATFRAAAAVQHANAADGVRIFNNLAATVYADSTAAHADMQGRRLKAVSDGLDHN
                     ATGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENTTAAATLGMGHSTWSE
                     NSANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRSTGADEHAEGSVNGTLM
                     QLGALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGWSGNSITEGTLVGLAG
                     LKLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGKTGARNMPHTRLVAGL
                     GADVEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT         1012 a         1019 c         1000 g          717 t
ORIGIN      
        1 cggagccgca gacagtacaa atagtacgga accgattcac ttgttaaaga atcgttctct
       61 ttgagctaag gcgcgacaac gccgtactgg tttttgttca tccactataa ctagggaaat
      121 tcaaattaag ttagaattat ccctatgaga aaaagccgtc taagccggta taaacaaaat
      181 aaactcattg agctatttgt cgaaagttca aatttccatt ttaaaacaat tagtaaaatc
      241 gagtttatcc tagttgtcca agacaacccc tataataata taattcaaaa tataaaaacg
      301 ggttacatct aaacattacg gaatttttat tccctcgcct gaattctatt gtcatattca
      361 aggagacctc atcatgcgaa cgaccccaac cttccctaca aaaactttca aaccggctgc
      421 catggcgtta gctgttgcaa caacactttc tgcctgctta ggcggcggcg gaggcggcac
      481 ttctgcgccc gacttcaatg caggcggtac cggtatcggc agcaacagca gggcaacaac
      541 agcgaaatca gcagcagtat cttacgccgg tatcaagaac gaaatgtgca aagacagaag
      601 catgctctgt gccggtcggg atgacgttgc ggttacagac agggatgcca aaatcaatgc
      661 ccccccgaat ctgcataccg gagactttcc aaacccaaat gacgcataca agaatttgat
      721 caacctcaaa cctgcaattg aagcaggcta tacaggacgc ggggtagagg taggtatcgt
      781 cgacacaggc gaatccgtcg gcagcatatc ctttcccgaa ctgtatggca gaaaagaaca
      841 cggctataac gaaaattaca aaaactatac ggcgtatatg cggaaggaag cgcctgaaga
      901 cggaggcggt aaagacattg aagcttcttt cgacgatgag gccgttatag agactgaagc
      961 aaagccgacg gatatccgcc acgtaaaaga aatcggacac atcgatttgg tctcccatat
     1021 tattggcggg cgttccgtgg acggcagacc tgcaggcggt attgcgcccg atgcgacgct
     1081 acacataatg aatacgaatg atggaaccaa gaacgaaatg atggttgcag ccatccgcaa
     1141 tgcatgggtc aagctgggcg aacgtggcgt gcgcatcgtc aataacagtt ttggaacaac
     1201 atcgagggca ggcactgccg accttttcca aatagccaat tcggaggagc agtaccgcca
     1261 agcgttgctc gactattccg gcggtgataa aacagacgag ggtatccgcc tgatgcaaca
     1321 gagcgattac ggcaacctgt cctaccacat ccgtaataaa aacatgcttt tcatcttttc
     1381 gacaggcaat gacgcacaag ctcagcccaa cacatatgcc ctattgccat tttatgaaaa
     1441 agacgctcaa aaaggcatta tcacagtcgc aggcgtagac cgcagtggag aaaagttcaa
     1501 tggctccaac cattgcggaa ttactgccat gtggtgccta tcggcaccct atgaagcaag
     1561 cgtccgtttc acccgtacaa acccgattca aattgccgga acatcctttt ccgcacccat
     1621 cgtaaccggc acggcggctc tgctgctgca gaaatacccg tggatgagca acgacaacct
     1681 gcgtaccacg ctgctgacaa cggctcagga catcggtgca gtcggcgtgg acagcaagtt
     1741 cggctgggga ctgctggatg cgggtaaggc catgaacgga cccgcgtcct ttccgttcgg
     1801 cgactttacc gccgatacga aaggtacatc cgatattgcc tactccttcc gtaacgacat
     1861 ttcaggcacg ggcggcctga tcaaaaaagg cggcagccaa ctgcaactgc acggcaacaa
     1921 cacctatacg ggcaaaaccg ttatcgaagg cggttcgctg gtgttgtacg gcaacaacga
     1981 atcggatatg cgcgtcgaaa ccaaaggtgc gctgatttat aacggggcgg catccggcgg
     2041 cagcctgaac agcgacggca ttgtctatct ggcagatacc gaccgatccg gcgcaaacga
     2101 aaccgtgcac atcaaaggcg atctgcagct gggcggcgaa ggtacgctgt acacacgttt
     2161 gggcaaactg ctgaaagtgg acggtacggc gattatcggc ggcaagctgt acatgtcggc
     2221 acgcggcaaa ggggcaggct atctcaaccg taccggacaa cgtgttccct tcctgagtgc
     2281 cgccaaaatc gggcgggatt attctttctt cacaaacatc gaaaccgacg gcggtctgct
     2341 ggcttccctc gacagcgtcg aaaaaacagc gggtagtgaa ggcgacacgc tgtcctatta
     2401 tgtccgtcgc ggcaatgcgg cacggactgc ttcggcagcg gcacattccg cgcccgccgg
     2461 tctgaaacac gccgtagaac agggcggcag caatctggaa aacctgatgg tcgaactgga
     2521 tgcctccgaa tcatccgcaa cacccgagac ggttgaaact gcggccgccg accgcacaga
     2581 tatgccgggc atccgcccct acggcgcaac tttccgcgca gcggcagccg tacagcatgc
     2641 gaatgccgcc gacggtgtac gcatcttcaa caatctcgcc gctaccgtct atgccgacag
     2701 taccgccgcc catgccgata tgcagggacg ccgcctgaaa gccgtatcgg acgggttgga
     2761 ccacaacgct acgggtctgc gcgtcatcgc gcaaacccaa caggacggtg gaacgtggga
     2821 acagggcggt gttgaaggca aaatgcgcgg cagtacccaa accgtcggca ttgccgcgaa
     2881 aaccggcgaa aatacgacag cagccgccac actgggcatg ggacacagca catggagcga
     2941 aaacagtgca aatgcaaaaa ccgacagcat tagtctgttt gcaggcatac ggcacgatgc
     3001 gggcgatatc ggctatctca aaggcctgtt ctcctacgga cgctacaaaa acagcatcag
     3061 ccgcagcacc ggtgcggacg aacatgcgga aggcagcgtc aacggcacgc tgatgcagct
     3121 gggcgcactg ggcggtgtca acgttccgtt tgccgcaacg ggagatttga cggtcgaagg
     3181 cggtctgcgc tacgacctgc tcaaacagga tgcattcgcc gaaaaaggca gtgctttggg
     3241 ctggagcggc aacagcatca ctgaaggcac actggtcgga ctcgcgggtc tgaagctgtc
     3301 gcaacccttg agcgataaag ccgtcctgtt tgcaacggcg ggcgtggaac gcgacctgaa
     3361 cggacgcgac tacacggtaa cgggcggctt taccggcgcg actgcagcaa ccggcaagac
     3421 gggggcacgc aatatgccgc acacccgcct ggttgccggt ctgggcgcgg atgtcgaatt
     3481 cggcaacggc tggaacggct tggcacgtta cagctacgcc ggttccaaac agtacggcaa
     3541 ccacagcgga cgagtcggcg taggctaccg gttctgacgg acaggaagca gacagccgca
     3601 aagatcacgg tctttgcggc tgtttcttat gaaaagaaga ccctattcca attgcctgct
     3661 tctattgttt caagacttct tccaaagatt cggcattaat cagatgtata gcggattaac
     3721 aaaaatcagg acaaggcggc gaagccgc
//
LOCUS       DAWKOG010000175         3709 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.175, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000175 DAWKOG010000000
VERSION     DAWKOG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.175"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            270..1391
                     /gene="dnaJ"
                     /locus_tag="WJK66_001895"
     CDS             270..1391
                     /gene="dnaJ"
                     /locus_tag="WJK66_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEZ7579549.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            1586..3604
                     /locus_tag="WJK66_001896"
     CDS             1586..3604
                     /locus_tag="WJK66_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEZ7579550.1"
                     /translation="MNKSLSGSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPAAERAEQDLSPKAMIFWVLAMMFVLGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSV
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIVVDLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGK"
BASE COUNT          777 a          830 c         1115 g          987 t
ORIGIN      
        1 aatagggggg aagtctgcag tcgcatcaaa tgccgtctga acatgcgttc gggcggcgtt
       61 tttataacaa aaacacttca tggcggttgg ttttatgcct gtctaagttt ttgtgtcgtg
      121 catacctgaa gatttcagac ggcatcggtt tatgctgtct gaaaagtgta ttccgtttca
      181 gtttgtaagc tatggcagtc tgtttgtctt gtgttttgcg caattgccct tattttgagc
      241 cgtgatttta ttttgaatta gatgaaaaaa tgagtaatca agatttttat gcgacgctgg
      301 gtgtggcaag aacagctacc gatgatgaga ttaaaaaagc ctaccggaaa ttggcgatga
      361 aataccatcc cgaccgcaat cctgacaata aagaggcgga agagaagttt aaagaagtac
      421 aaaaggcgta tgaaactttg tccgacaagg aaaagcgcgc tatgtacgac cagtatggtc
      481 atgcggcgtt tgaaggcggc ggacaggggg gcttcggagg gtttggcgga tttggcggtg
      541 cgcagggttt tgactttggg gatattttca gccaaatgtt tggaggcggt tcggggcgcg
      601 cccagcctga ttatcagggt gaggacgttc aagtcggtat cgaaatcacg cttgaagaag
      661 ccgcaaaagg tgtgaagaaa cgcatcaata ttccgactta tgaagcgtgt gatgtctgta
      721 acggcagtgg cgcgaaaccg gggacatccc cggaaacctg cccgacttgc aaaggttcgg
      781 gtacggtgca catccagcag gcgattttcc gtatgcagca gacttgtccg acctgccacg
      841 gtgcgggcaa acacattaaa gaaccttgcg tcaaatgccg tggcgcgggg cggaataagg
      901 cggtcaagac ggtggaagtc aatattcccg ccggtatcga tgacgggcag cgtatccgtt
      961 tgagcggcga aggcggaccg ggtatgcacg gtgcgcctgc tggcgacctg tatgtaaccg
     1021 tccgcattcg ggcgcataag attttccaac gcgacggtct ggacttgcat tgcgaactgc
     1081 cgatcagttt tgccacggct gctttgggcg gggagttgga agtgccgacc ttggacggaa
     1141 aggtcaagct caccgtcccc aaagaaaccc aaaccggcag gaggatgcgc gtgaagggta
     1201 agggtgtcaa atctttacgc agcagcgcga ccggcgattt gtactgccat attgttgtcg
     1261 aaacgcctgt caatttgacc gaccgtcaaa aagagctttt ggaagaattt gagcggattt
     1321 ctaccggctt ggaaaaccaa acaccgcgca agaaatcgtt tttagacaag ctgcgcgatt
     1381 tgtttgattg attttaaggt tcggaaacaa gcagccgtat cggggaatct ccctgatacg
     1441 gctgttttgt ttgtttaaaa atagttttta ttttcaatgg ggtatggggc agggtgggat
     1501 aactgttttt aactgttctt tttaaaactt gacatcatgg cgcgatgcca acaatatgtg
     1561 aacgtctgtt gtcaaaggaa gaataatgaa taaatcttta tccgggtcgg tagaagaata
     1621 ccgcgaactg acgctccgag gcatgatact cggtgcattg atcactgtaa tttttactgc
     1681 gtccaacgtt tacctcggtt tgaaagtcgg gctgaccttt gcctcgtcga ttccggcggc
     1741 ggtaatttcg atggcggttt taaagttttt caaaggcagc aatattttgg aaaacaacat
     1801 ggtgcagacc caagcttcgg cggcgggtac gctttcgacc atcatcttcg tcctgcccgg
     1861 tttgctgatg gcgggctact ggagcggttt cccgttctgg cagacgacgc ttttatgtat
     1921 tgccggcggg attttggggg tgattttcac cattccgctg cgttacgcga tggtggtgaa
     1981 aagcgatttg ccttatccgg aaggtgtggc ggctgctgaa attttgaaag tgggcggtca
     2041 tgaagaaggg gataaccgtc agggcggcag cggcatcaaa gagctggcgg ccggcggtgc
     2101 gttggcggga ttgatgagct tttgcgccgg aggtctgcgc gtgattgccg acagcgcgag
     2161 ttattggttt aaaagcggta cggcgatttt ccagctgccg atgggctttt cactggcatt
     2221 gttgggcgcg ggctatttgg tcggactgac gggcggtatc gccatcctgt tgggcatttc
     2281 gattgcttgg ggcattgccg tgccgtattt ctcctcacac attccgcaac cttccgatat
     2341 ggaaatggcg gcgtttgcga tgaagctgtg gaaggagaaa gtgcgtttta tcggtgcggg
     2401 gactattggc attgcggcgg tttggacgct gttgatgctg ctcaagccga tggtggaagg
     2461 catgaagatg tcgttcaaga gttttggcgg cggtgcgccc gctgcggaac gcgccgaaca
     2521 agatttgtcg cctaaagcca tgattttttg ggtgctggcg atgatgtttg ttttaggcgt
     2581 gtcgttttac cactttatcg gcgattcgca cattacgggc ggcatggctt ggcttttggt
     2641 ggtcgtttgc acgcttttgg cttccgtcat cggctttttg gtcgccgccg cctgcggtta
     2701 tatggcaggt ttggtcggct cgtcttccag cccgatttcc ggcgtgggca tcgtgtccgt
     2761 cgtcgttatt tcactggttt tgctgctggt aggcgaatcc ggaggtttgt tggcggatga
     2821 ggctaaccgc aaatttttgc tggcactgac tttgttttgc ggctcggcag taatctgcgt
     2881 ggcttcgatt tccaatgaca acctgcaaga cttgaaaacc ggctacctgc tcaaagccac
     2941 gccttggcgg cagcaagtcg ccctgattat cggctgtatc gttggtgcgc tggttatttc
     3001 gcccgtgttg gaactgcttt acgaagccta cggctttacc ggcgcaatgc cgcgcgaagg
     3061 catggatgcg gcgcaggctt tggcagctcc tcaagcgact ttgatgacga ccatcgcgtc
     3121 gggcattttc gcccacaacc tcgaatgggt ctacatcttt accggcatcg cgatcggcgc
     3181 ggtattaatc gtcgtcgatt tggtgttgaa aaaatcatca ggcggcaaac ttgccctgcc
     3241 cgtccttgcg gtcggtatgg gtatttatct gccgccgtcc gtcaatatgc ccatcgtggc
     3301 aggcgcggtg ttggcggcgg tgttgaaaca catcatcggt aaaaaagcgg aaaaccgcga
     3361 aggccgtctg aaaaacgccg agcgtatcgg aaccttgttc tccgccggcc tgattgtcgg
     3421 tgaaagcctg atcggtgtga ttatggcgtt tattattgcc ttctccgtga ccaacggcgg
     3481 ctcggatgcg ccgcttgcgt tgaatctgca aaactgggat gccgccgctt cttggttggg
     3541 tttggcgttc ttcgttaccg ggatgttttt ctttgcacag cgcgtactga aggcgggcaa
     3601 gtaggctgtc ggaaaaaatg ccgtctgaag cgttcagacg gcatttttat ctggaaagcg
     3661 gaaggcggag cttttcggct tgcgcccacg ttttgccggc aaggtcttt
//
LOCUS       DAWKOG010000176         3657 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.176, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000176 DAWKOG010000000
VERSION     DAWKOG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.176"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(121..276)
                     /gene="rpmG"
                     /locus_tag="WJK66_001897"
     CDS             complement(121..276)
                     /gene="rpmG"
                     /locus_tag="WJK66_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEZ7579551.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(308..541)
                     /gene="rpmB"
                     /locus_tag="WJK66_001898"
     CDS             complement(308..541)
                     /gene="rpmB"
                     /locus_tag="WJK66_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEZ7579552.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(791..2317)
                     /gene="farB"
                     /locus_tag="WJK66_001899"
     CDS             complement(791..2317)
                     /gene="farB"
                     /locus_tag="WJK66_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HEZ7579553.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFAVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     SLVVFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKCRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGIIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGVGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     HGVSDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHNGGGGGH
                     "
     gene            complement(2341..3495)
                     /gene="farA"
                     /locus_tag="WJK66_001900"
     CDS             complement(2341..3495)
                     /gene="farA"
                     /locus_tag="WJK66_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HEZ7579554.1"
                     /translation="MDTHTDETKLQNTQAKRKRRLTALTLLFALAAAAAGSAFFLWWQ
                     HEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVLAYERAK
                     NELVQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSAEELAHA
                     RAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQRTQIRA
                     PADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPAELVSDL
                     YGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDVDRHPLR
                     IGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
BASE COUNT          901 a         1022 c          917 g          817 t
ORIGIN      
        1 tctatccttg gcagacggtt tgcagcagat tcgcatacgg cagatgtttc aagcggaaag
       61 gaaacacgat aaaaataaca aagcctccga gcagtcggag gctttgcttt caaactgaaa
      121 ttatttcagt ttagtttctt tatacactac gtgtttgcgg gcaactgggt caaatttttt
      181 gatttccaat ttgccgggca tagtgcgttt gtttttggta gtggtgtaga agtgaccagt
      241 acctgcactg gattccagtt tgattttatc gcgcattgca gtaatcctta attaaatagt
      301 gtttaaatta agcttcgccg cgagcacgca aatcagccaa tacgacatca atgcctactt
      361 tgtcgatggt acgcagtgca gcgttggaaa cgcgcaggcg aacccagcgg ttttcacttt
      421 ctacccaaaa acgacgtgat tgcaagttgg gcaaaaaacg gcgtttggtt ttgttgttgg
      481 cgtgcgatac gttgttgccg gacatcgggc gtttgccggt cactttgcaa actcgtgcca
      541 taatcagtct ccaaacttct aaaatagtaa aacgcgattt ataccacgaa aaacagtgtt
      601 agttcaagtg ttttaaagaa aaacggctgt tttgcgcgga taatccttcc gattgtcgca
      661 tctgtggaac gtttgcaggt gcgaaacaga tggggcgcga ttatagcgga tttcgggaac
      721 tgctgcattc aaaatgccgt ccgaacatat ttccagtatt ttcagacggc atttcaagtt
      781 ttcaaatccc tcaatgtcca ccgccgccgc cgttgtggaa cggcggtttt gccagccata
      841 tgacgggtat catgataatg aataacaagc tgcccgccat aaagatttcg ttcgagccga
      901 taataaaacc ctgctgggta atggtattgt tgatgatgcc tagggtttga atgtcggaaa
      961 cgccgtgctg ggacaaatga gcggccgttt cgtgcaatgt tgcggaatag ggcgtgatgt
     1021 gttcggcaaa gcgcgtgtgg tgcaacgctt cgcgccgttc ccacagggtg ctgacgacgg
     1081 atacgccgac accgcccatc agcacgcgca ggaaattcga caggctgccg gcggcggcaa
     1141 tctgcccgcc cttcatgtgc gacagtgtga tggtagtcag cggcaggaag aacatggcaa
     1201 cgcctactcc ctgccaaaac tgcggccaga tgacgttgcc gatatccatg tcggcataaa
     1261 aatcggtgcg ccaatagaaa gtgaaggcaa aggtcaggaa gctggcggtt acgagcaggc
     1321 gcatatcgat tttattgccg aacctgccga ttaacggaga caggaagacg ggcaggatgc
     1381 cgacgggcgc ggcggcaagc cccgcccacg tggaggtata gcccaggttg gtctgcaaca
     1441 ctaacggcag cagggtcagc gtccccatat acaccataaa acccaatgat gtggcaatga
     1501 tgccgacggt aaaattccga tctttaaaca gcgataaatc gacaatcgga tatttttctc
     1561 ccaattccca aacaataaaa tacgacaagc acaccagtgc gactacgccc aaggtaatga
     1621 tttctccaga ggcgaaccag tcgagttcct tacccctgtc caacatcatc tgtaacgcgc
     1681 cgataccgac taccatcaat gtaagcccga catagtcggt cggcatttta acggtttccg
     1741 tttcccgaca tttcaaatgt ttccatgtaa tccatgccga tatgataccg atagggatat
     1801 taatgaagaa aatccaaccc caatgccagt ttccggaaat ccagccgccg agtatcggcc
     1861 cgagaacagg ggcgacaacg acggtcattg cccacaatgc cagtgccagc gtccgttttg
     1921 cgggcggata ggatgccatt aacaggcttt gcgacaaggg aatcagcggc ccggcgataa
     1981 agccctgcaa gatgcggaaa acaaccagcg actgaaggtt gggggcaata ccgcacagcc
     2041 acgatgtgat gacgaaaccg acagcggcgg cggtaaacaa tttgacctcg ccgatacgtt
     2101 ttgccaaaaa gcccgttaac ggcacggaaa cggcgtttgc cacagcaaaa gaagtaatga
     2161 cccacgtccc ctgcgtggtt gccgcaccga ggttgccggc gatgacggga acggcgacat
     2221 tggcgatagt cgtatctaaa acttccataa atacggcaag ccccaaagac agcgtaaccc
     2281 acgccaatgc cgcaccctta agcggtggat aatccattgt gtcctccgtt tcagacggca
     2341 tcagggcgcg gattgcccga ggatttcatc gaccgtccga tcgacttccg accagtcggt
     2401 actttccatt tccggcaatg ccgcacccgg cgtttttgaa acaggcgcgc ctgcggcgga
     2461 agtatccact ttaaccgtca tcgacaaacc gatacgcaac ggatgcctgt ccacatcttc
     2521 gcggttcagc acgatacgga cggggacgcg ctgcaccact ttaatccagt tgcccgttgc
     2581 gttttgcgcc ggaatcagcg aaaacgcgct gcccgtacct gccgaaaaac ctgccaccct
     2641 gccgcgataa acaatttgtt tgccgtacaa atcggacacc agctcggcag gctgtccgat
     2701 tttcatatgc cgcaactgcg tctctttaaa attagcatcc acccacacat ccgacagcgg
     2761 caccaccgcc atcagcggcg cgcctgccgc cacctgctgc ccgacctgca ccgaacgctt
     2821 cgccacctga ccgtccgccg gcgcgcggat ttgcgtccgc tgaaggttca accacgcatc
     2881 tttcaacctg ccgattgccg tctgaaccgc cggctgttcg cgcaaagaaa cctgaccgcc
     2941 caaagccgca cgtgccgaag attcttccgc caaagccgct ttgaccgccg cctgcgcctg
     3001 agacactgcc gcacgggcgt gtgccagctc ttcggcggac accgcgcccg attccgccaa
     3061 agcagaccgg cggcgcaaat catcctgtgc gcgtgccaaa tccgcccggc gcaaggcaac
     3121 ctgcgccccc gcctgcgaag tggcggcatt ttgccggcgg ttttgccgca ccgcctgaac
     3181 cagctcgttt tttgcccgct cgtaagccag cacatcatta tcgtcgtcca ataccgccag
     3241 cacgtcgcct tttttcacgg catccgtatc gtcgtgcaaa accttccgca ccgtaccgcc
     3301 cttttgcggc gtaacctgaa ccacgcgtcc ggcaacataa gcgtcttccg tttcctcttc
     3361 gtgctgccac cataaaaaaa acgccgaccc ggcggctgcg gcggcaagcg cgaacagcag
     3421 cgtcaatgcc gtcaggcggc gtttgcgttt ggcttgcgtg ttttgaagtt ttgtttcgtc
     3481 cgtgtgcgta tccataagat tgggttccga atttcctgat ttcatataaa caatgttctt
     3541 tcaataggaa gcagattcta tatccgcgca ataacgcaat caagtcagta ttttagatgc
     3601 aatattcaaa aaacaacgct tcaggcggca aaactgaaaa tatatttcgt ttatata
//
LOCUS       DAWKOG010000177         3559 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.177, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000177 DAWKOG010000000
VERSION     DAWKOG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.177"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(120..974)
                     /locus_tag="WJK66_001901"
     CDS             complement(120..974)
                     /locus_tag="WJK66_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEZ7579555.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(1098..1826)
                     /gene="rpsB"
                     /locus_tag="WJK66_001902"
     CDS             complement(1098..1826)
                     /gene="rpsB"
                     /locus_tag="WJK66_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEZ7579556.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQEAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDYRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAEGGFNKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            complement(2003..2236)
                     /locus_tag="WJK66_001903"
     CDS             complement(2003..2236)
                     /locus_tag="WJK66_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579557.1"
                     /translation="MWHIVAIGYLFVAVMFSAAQPSIARALIYLVFWAVLPTVFTVFV
                     VTVRRRNRLMRQQEQAEFEQQRAQRQKDSGTKP"
     gene            2314..2895
                     /locus_tag="WJK66_001904"
     CDS             2314..2895
                     /locus_tag="WJK66_001904"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEZ7579558.1"
                     /translation="MRNEEKRALRRELRGRRSQMGWDVRAAATVKINRLLKRYIKKGR
                     KIGVYWPMGKELRLDGFVRAAQKRGVKLYLPYIEPRSRRMWFTPYPADGVKQERKRGR
                     AKLHVPQFAGRKKRVHDLNLLLVPVVGMDRLGYRLGQAGGYYDATLAAMKYRLQAKTV
                     GVGFACQLVGTLPREAHDLPLDGFVSEAGILCF"
     gene            2956..3546
                     /locus_tag="WJK66_001905"
     CDS             2956..3546
                     /locus_tag="WJK66_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ7579559.1"
                     /translation="MSEKIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
BASE COUNT          788 a          824 c         1022 g          925 t
ORIGIN      
        1 ttttgttttc agacggcctt tatattcggt cgtcttaaaa taggtaccgc aaacggtttt
       61 ctcaaaaaaa agcaccctga ttcgtttgga atccaggtgc tttctttttc ataagtgcct
      121 tacactttgg cggctgcggc tacttcggct gcgtagtcga caacggcttt ctcgataccg
      181 tcgcctactt tgtagcggac gaagctgacc acttcagtgc cgttttcttt ggcgaattgg
      241 gcaacagttt gatcggggtt catcacgaat gcttggccgt tcagagtgat ttcagccaag
      301 aatttgcgga tacggccttc aaccatttta gcggcgatgt cggcaggttt gccggaagcg
      361 atggcttgtt cagtgtagat gtggcgttct ttttcaacgg tttcagcatc tacttcggct
      421 tcgcttacgc attgtggttt agcggcaaca atgtgcatac cgattttgcg tgctacgtct
      481 tcagagcctt tgtactcaac caatacgcct tcggtagcca atgcgccgtg gatgtaggca
      541 accagttggt tggcagtgtc gatcacttgg aagcgacgga cagacatgtt ctcgcccaat
      601 ttggcgatga tggctttgcg ttctgcttca accagttcgc tcagttcttc aacagaagcc
      661 ggttttttct cggcagcagt tttagcaacg aagttggcga attctacgaa gccggcatct
      721 ttagcaacga agtcggtttc gcagtttact tcaaccaatg caccgacatt gccgttgatc
      781 gcgtaagcca atacgccttc ggcagcagta cggccggcca gtttaccggc tttcgcacca
      841 gatttaatac gcaggatttc ttcggctttg tcgaagttgc cttcagcttc aaccaaggct
      901 tttttgcatt ccatcatgcc caggccggta gcggcgcgca ggtcggcaac catttttgca
      961 gtaatttctg ccattttgaa tctcctagat ttttgggaac acggccattg ccgtgtttgg
     1021 aaatagtggc cgtctgaaac ggaattcaga acgggatttg agaaaagggg cataacgccc
     1081 ctcttctgtg taccgaatta ctcggcagca gcttcttggg cagcggctac agtttcttgc
     1141 agcgcttggt ttttgccttc caaaactgcg tcagcgatgc cgcggcagta caggcggatg
     1201 gctttggcgg agtcatcgtt accggggata acgtatttca cgccgtcagg gctgttgttg
     1261 gtatcgacta cggcgataac agggatgccc aatttttcag cctcaaccag agtacctttt
     1321 tggtagccgg tatcgataac gaaaatcgcg tcaggcaggc ctttcatgtt tttgataccg
     1381 cccaaagaac gttccagttt ttcaacgtcg cgttgcattt ccagaatttc ttttttgttg
     1441 aaaccgcctt cggcagcatt ttccaaggct gcggtttttt cttccaggcg tttgatggat
     1501 tgcttaacgg ttttgtagtt agtcagcata ccacccaacc agcggtaatc aacgaaaggc
     1561 ataccggcac gggtcgcttc ttcgcggatg atgtcgcggg cttggcgttt ggtacctacg
     1621 aacaatactg tacctttgtt ggcaaccaga cgacgtacgg cttcttgcgc ctcttggaac
     1681 atcggcaggg ttttttccaa gtttacgata tggattttgt tgcgcgcgcc gaaaatgtat
     1741 tgtgccattt tcggattcca gaaacgggtt tggtggccga agtggacacc ggcttcaatc
     1801 atctgacgca tagtaatttg agacattttg atttccttga aagggttaag ggcacacatt
     1861 caatcgcata gaacttgata ggcgatgcct attgtcaagc acccttcggc gaaagtgggc
     1921 ataagtttta aaaaataaaa gagtgtttcc gaaatggaaa actgacggat tatagcggat
     1981 aagaggccgt ctgaaaaggg attcagggtt ttgtgccgct gtctttttgc cgttgtgcac
     2041 gctgctgttc gaattctgcc tgttcctgct gcctcatcag gcggttgcgg cggcggacgg
     2101 ttacgacgaa gaccgtgaac acggtgggca gaaccgccca aaaaaccaaa taaatcaagg
     2161 cgcgcgcaat actcggctgc gcggcggaaa acatcacggc aacaaaaaga tagccgatgg
     2221 cgacaatgtg ccacattgtg tgcgtcctgt ttgaatgtta acaaaatcgt tataatcgca
     2281 acattctacc gcaatcctga aaggccgtct gaaatgagga atgaggaaaa acgcgccctg
     2341 cgccgcgaat tgcgcgggcg gcgttcgcaa atggggtggg acgtgcgggc ggcggcaacg
     2401 gtaaaaatca accgcctgct caaacgttat attaaaaaag ggcggaaaat cggcgtgtat
     2461 tggccgatgg gcaaggaatt gcgtttggac ggctttgtcc gcgcggcgca aaaacgcggt
     2521 gtaaaacttt atctgcctta tatcgaaccg cgttcgcggc ggatgtggtt tacgccgtat
     2581 cctgccgatg gagtaaaaca agaacgcaag cgcggtaggg cgaagctgca tgtccctcag
     2641 tttgcaggtc ggaaaaagcg tgtgcatgat ttgaacctcc tgcttgtgcc agtggtcggt
     2701 atggacaggc tgggctaccg cttggggcag gcaggcggct attacgatgc gacgcttgca
     2761 gcgatgaaat accgtttgca ggcaaaaacc gtgggcgtgg gctttgcctg ccaactggtc
     2821 ggcacgctgc cgcgcgaggc gcacgacctg ccgctggacg gttttgtgtc ggaggcgggg
     2881 atattgtgtt tttagccgtt cagacggcat tccgggcgga aatgccgtct gaaggcggaa
     2941 tattgggaga aaaggatgtc ggaaaagata atactgccgg tcttgtcatt gggcggcgtt
     3001 cggctcgaac cgttggatgt gcatcatgaa acgggtttgc gcgaggcggt ttgcgatggg
     3061 gaggtttgga agctgggggt aacgtccgcg ccccatccgg atagggtggc agactatatc
     3121 gggacagcat tggcaacgcg tttggcgttt gcggttgtcg atgaagaggc gggcagggtg
     3181 gtcgggacaa cggcgtatta tcattttgaa ccgcagattc cgcgtttgga tatcggattc
     3241 acatggtatg cggcatcggc acggcggaca cggataaaca cctgttgcaa aatcatgctg
     3301 ttggattacg catttgatgt tttggcttgc cgttgcgtgg gatggcgcac ggatattctg
     3361 aaccttgcct cgcaacgtgc catcgaacgg ctgggtgcgg aaaaagacgg cgtgctgcgt
     3421 atgcatatgc tccgtaaaga cggcagtgtg cgcgatacgg tcgtgtacag tatgctgagg
     3481 gaagattggt gcaaaaacag ggaaatcctg accggcaggt tggcagggta tggcgtgcag
     3541 gtgtgaggca gtattttca
//
LOCUS       DAWKOG010000178         3494 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.178, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000178 DAWKOG010000000
VERSION     DAWKOG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.178"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            226..1101
                     /locus_tag="WJK66_001906"
     CDS             226..1101
                     /locus_tag="WJK66_001906"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEZ7579560.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAIPMILYNVPGRTVVSMNNETILRLAEIPNIVGV
                     KEASGNIGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARELNNRLIPIYDTMFCEPSPAAPKWAVSALVRCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            1110..2306
                     /gene="bamC"
                     /locus_tag="WJK66_001907"
     CDS             1110..2306
                     /gene="bamC"
                     /locus_tag="WJK66_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEZ7579561.1"
                     /translation="MPSEPFGRHNATNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGGKDKDTTMWQPSASDPNLEAAFLTRFMQYLGVDGQQAENASAKKPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTALALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(2363..3295)
                     /gene="pip"
                     /locus_tag="WJK66_001908"
     CDS             complement(2363..3295)
                     /gene="pip"
                     /locus_tag="WJK66_001908"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEZ7579562.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETVWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEEVDEDAYASL
                     AIARLENHYFVNGGWLQGDRAILNNIGKIRHIPTVIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLADALVQAVEDIFPRLL"
BASE COUNT          933 a         1158 c          776 g          627 t
ORIGIN      
        1 aggcaacacc gtactggttt ttgttaatcc actataaatc cgacggcacc ggcataaacc
       61 ggcgaatata ataaaatgat ttcaaacagg aaaaatcaat ttccaccata cctttcggcg
      121 caaacgcccc acatccgcaa cccgccggat ttcacacctg cggtaaagta ggctacaatc
      181 ggaagcatta ccgcccccta caccacaact gataaaaggt tctgtatgtt acaaggtagc
      241 ctggttgccc tgattacccc gatgaatcaa gacggcagca tccattacga acaactccgc
      301 gacttaatcg actggcacat tgaaaacggc acggacggca tcgttgccgt cggcacgaca
      361 ggcgaatccg ccaccctctc cgtcgaagaa cacaccgccg tcatcgaagc cgtcgtcaaa
      421 cacgttgcca aacgcgtccc cgtcatcgcc ggcacagggg caaacaacac cgtcgaagcc
      481 atcgcccttt cccaagccgc cgaaaaagcc ggggcggact acaccctctc cgtcgtcccc
      541 tattacaaca aaccttcgca agaaggcatg taccgccatt tcaaagccgt tgccgaagcc
      601 gccgcgattc cgatgatact ctacaacgtt cccggcagaa ccgtcgtcag catgaacaac
      661 gaaaccatcc tgcgccttgc cgaaatcccc aacatcgtcg gcgtgaaaga agccagcggc
      721 aacatcggca gcaacatcga actcatcaac cgcgcgcccg aaggcttcgt cgtcctgtcc
      781 ggcgacgacc acaccgccct accgttcatg ctctgcggcg gacacggcgt tattaccgta
      841 gccgccaacg ccgcacccaa gctctttgcc gatatgtgcc gcgccgcgct tcaaggcgat
      901 atcgccctcg cacgcgagct caacaaccgg ctgattccga tttacgacac catgttctgc
      961 gaacccagcc ccgccgcacc gaaatgggcg gtatccgccc tggtcagatg cgaaccgcac
     1021 gtccgcctgc cgctcgttcc gctgacggaa ggcggacagg caaaagtccg cgccgccctg
     1081 aaagcctcag gacaacttta aatatagcca tgccgtccga accgttcgga cggcataacg
     1141 caacaaacac tttaatatcc atcacacagg atgacacgat gacccatatc aaacccgtca
     1201 ttgccgcgct cgcactcatc gggcttgccg cctgctccgg cagcaaaacc gaacagccca
     1261 agctcgacta ccaaagccgg tcgcaccgcc tgatcaaact tgaagtccca cctgatttga
     1321 acaaccccga ccaaggcaac ctctaccgcc tgcctgccgg ttcgggcgcc gtccgcgcca
     1381 gcgatttgga aaaacgccgc acacccgccg tccaacagcc tgccgatgcc gaagtattga
     1441 aaagcgtcaa aggtgtccgc ctcgagcgcg acggcagcca acgctggctt gtcgttgacg
     1501 gcaaatcccc cgccgaaatc tggccgctcc tgaaagcctt ctggcaggaa aacggcttcg
     1561 acatcaaatc cgaagaaccc gccatcggac aaatggaaac cgagtgggcg gaaaaccgtg
     1621 ccaaaatccc ccaagacagc ttgcgccgcc tattcgacac agtcggtttg ggcggcatct
     1681 actccaccgg cgagcgcgac aaattcatcg tccgtatcga acagggcaaa aacggcgttt
     1741 ccgacatctt cttcgcccac aaagccatga aagaagtgta tggcggcaaa gacaaagaca
     1801 cgaccatgtg gcagccttcc gcttccgacc ccaaccttga ggccgctttc ctgacgcgct
     1861 ttatgcaata tttgggcgtt gacggacagc aggcggaaaa cgcatcggca aaaaaaccta
     1921 cccttcccgc cgccaacgaa atggcgcgta tcgaaggcaa aagcctgatt gtctttggcg
     1981 actacggcag aaactggcgg cgcaccgcgc tcgccctcga ccgcatcggg ctgaccgtcg
     2041 tcggtcaaaa caccgaacgc cacgctttcc tggttcaaaa agccccgaac gaaagcaatg
     2101 cagttaccga acaaaaaccc ggcctgttca aacgcctgct gggcaaaggc aaagcggaga
     2161 aacctgccga acagccggaa ctgattgtct atgcagaacc tgtcgccaac ggctcgcgca
     2221 tcgtcctgct caacaaagac ggcagcgcat atgccggcaa agacgcatcc gcactgttgg
     2281 gcaaactcca ttccgaactg cgttaaaccg ttcaaaacaa aagcagcttc caaacagaag
     2341 ctgctttttt gtgcggaact ttttacaaca accgaggaaa aatatcctca accgcctgaa
     2401 ccaacgcatc cgccaaaggc ggatcgaacg cacgatgccc tgcctgaacc accctcaatt
     2461 ccgcttccgg aaaggctttc gacagcgccc acgcactctg catcggcgta cacaaatcat
     2521 accgcccctg tacgataacg gtcgggatat gccgtatttt gccgatattg ttcaaaatcg
     2581 ccctatcgcc ctgcaaccag ccgccgttga caaaataatg gttttccaaa cgcgcgattg
     2641 ccagcgaggc ataagcatct tcatccactt cctccggctc gaaacggatc agatagcttt
     2701 cccaatccgc ccaagccttc gcggcagaca gacacacttc ttcatcttga tgaaacagca
     2761 atccgtgata cgcctcaatc agccggttcc gccgattttc agcaatcggc gcgacaaatt
     2821 tttgccattg ttccggataa atccggctca caccgcccgc ctcgttcagc cacaccgttt
     2881 cagacggtct gcacaaaaat atcccgcgca acaccaatcc ctttacccgt tccggatggg
     2941 tttgggcata agccagcgac aaagtgctgc cccacgaacc gccgaacacc agccatttcc
     3001 cgatacccag catttcacgg actttttcaa tatccgccac caaatcccaa gtcgtattgt
     3061 cttccgcaca agcatacggg cgcgaacgtc cgcaaccgcg ctggtcgatg atgacgatgc
     3121 ggaacacatc gggattgaaa aaaccccgac attcaggcga agcccccgcg cccggcccgc
     3181 catgtaaaaa aataaccggc acaccgtcag gattacccga ctcttcccaa taaatttgat
     3241 gaatttcaga tacctgcaag tatccgctat gaaaaggctg ttttatctca tacatcatct
     3301 tctcccatat tgcttctgac aatccgaaca tcccgacacg cgacatcccg cacaaaacac
     3361 aagccgcccc gaaaggcaaa tgccgaaaaa tcgcgatata gtggattaac aaaaatcagg
     3421 acaaggcgac gaagccgcag acagtacaaa taatacggaa ccgattcact cgtgcttcag
     3481 caccttagag aatc
//
LOCUS       DAWKOG010000179         3456 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.179, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000179 DAWKOG010000000
VERSION     DAWKOG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.179"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            207..1679
                     /gene="pyk"
                     /locus_tag="WJK66_001909"
     CDS             207..1679
                     /gene="pyk"
                     /locus_tag="WJK66_001909"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEZ7579563.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRTGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQVEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
     gene            1989..3383
                     /locus_tag="WJK66_001910"
     CDS             1989..3383
                     /locus_tag="WJK66_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ7579564.1"
                     /translation="MTPSALKKTVLLLGTAFAAASAQASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLTKLDSSQISVNANIVLPSIHYEADSATDFTGLPVQGSKSGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNERHSFGAGIIAQHTSAELRKYADWGIMEKAKALKETPPNPTKAAQIKADGHA
                     DVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADDAMAKQLWDANK
                     LALLGYTPSEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFEKE
                     KTIVNGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAFDKSPVRNADYRMNSL
                     PDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTSYRTAKASGNDVDSKGASSARFKN
                     HADIIGLQYTYKFK"
BASE COUNT          896 a         1058 c          867 g          635 t
ORIGIN      
        1 aataaattta gcctaagtca taaaaccgtg taattttgcg ccaaaaaagg cggtttgcag
       61 gtaaaatatc aaactattaa tttaaatcat atttttacag aatattccgc cgcgttcatc
      121 aaatggacat caaaccggtt ccaaatttct gtaattttgt aacaaaatac cgcaaaacac
      181 ccgattgaga ccaaaaggac tttcatatga accagacaag ccgcgatctg acccgcatca
      241 gccacaacac taaaatcgtc gccacccttg ggccgggcag caacaacgtc gaactgttgg
      301 aagacatgat ccgcgtcggc ggtctgaacg tcgtccgctt caacttcagc cacggcacgc
      361 ccgaattcca tcaggaaaac gcccgcatcg tgcgcgaggc ggcaaaacgc accggacagg
      421 aaatcgccat cattgccgac ctgcagggcc cgaaaatccg cgtgggcaaa atcgccggcg
      481 gcggcatcga attgaacaaa ggcgaaacgc tggtactgga tgccgcgctc gaaggcgaag
      541 gcacgcgcga ggcggtcggt ttggactacc gcgacctgcc cgacgacgtt gccgcaggcg
      601 atgtcttgtg gctggacgac ggcctgctga ccctgaccgt ggaatccgtc gaaggcagca
      661 ggattatcac aagggtggaa aacagccacg tcctgaaaag caacaagggc atcaacaaac
      721 gcggcggcgg tctgtccgca ggcgcgttga ccgaaaaaga cttccgcgac ctgaaaaccg
      781 cgattgccat cggttgcgac tatctcgcca tcagctttgt gaaatccgcc gaagatttgc
      841 acatcgcgcg cgccaaagtc gaagaagaaa tgaaaggcag tacggccgtg cgccccggtt
      901 tggtttccaa aatcgaacgc gtagaagcga ttgaaaacct tgatgaaatc atcctcgccg
      961 gcgacggcat tatggttgca cgcggcgact tggcggtcga agtcggacac gccgccgtcc
     1021 ccgccctgca aaaacggatg atccgccgcg cccgcgagtt gcgccgcttc agcattacgg
     1081 cgacgcaaat gatggaatcg atgattacca accccgtgcc gacccgcgcg gaagtcagcg
     1141 atgtggcaaa cgcggtattg gacggcaccg atgcggtgat gtgttccgcc gaaaccgccg
     1201 tcggcgcgta tccgtttgaa accgtcagcc aaatggcgat tatctgcgcg gctgcggaaa
     1261 aagagcagga ttcgctcaac ggcgttgccg aacaggtcga gtatcccgaa gcggtcagca
     1321 ccaacctggc ggttgccggc ggtgcggtca gcgtggcgcg cgcggttcac gccaaagcca
     1381 tcgtcgccct gaccgaaagc ggttcgaccg cctttgaaat cagccgccac aacatcaccc
     1441 tgccgatttt cgcgctgacc ccgagcgttt ccgcccaacg ccgtatggcg atgtaccggg
     1501 gcgtgcgccc gctgattttg gcaaccagca ccgaccacga cacggcgtta aacgaagtcg
     1561 aaaccatgct ggtggaacac aacatcctgc attccggcga ccaatacatc attaccagcg
     1621 gctcgcaaat gcgcgaatcc ggttcgacca atacgctgga agtgctgcgc gtcaaataat
     1681 ccgccctgag tggaaaatgc cgtctgaagt cgatgcccga ggcttcagac ggcatttttt
     1741 gcggcggcgc ggcggttcgg gcaaactctg aaaaaattgt gcaaatccgg caacatcgga
     1801 taaaatcgag tacctatact aaagcgaaac aaggcatttc cgactgcctt ttttatagtg
     1861 gattaaattt aaaccagtac ggcgttgcct tgccgtacta tctgtactgt ctgcggcttc
     1921 gtcgccttgt cctgattttt gttaatccac tatatttatc caccgtccgc ctttttacgg
     1981 aaaccgaaat gaccccttcc gcactgaaaa aaaccgtcct actgctcggc actgcctttg
     2041 ccgccgcatc cgcacaagcc tccggctacc acttcggcac acagtcggtc aacgcgcaaa
     2101 gcacggcaaa tgccgccgcc gcagaagccg ccgacgcatc gaccatcttc tacaaccctg
     2161 ccggcctgac caaactcgac agcagccaga tttccgtcaa cgccaacatc gtgctgccca
     2221 gcattcatta tgaggcggat tccgccaccg actttaccgg gcttcccgtc caaggttcga
     2281 aaagcggcaa aatcaccaaa accacggtcg cgccccacat ctacggcgca tacaaagtca
     2341 acgacaatct gaccgtaggc ttgggcgtgt acgtcccctt cggttctgcc accgaatacg
     2401 aaaaagattc cgtgttgcgc cacaacatca acaaactcgg tctgaccagc atcgccgtcg
     2461 aacctgtcgc cgcgtggaaa ctcaacgaac gccattcctt cggcgcaggc atcatcgccc
     2521 aacatacttc cgccgagctg cgcaaatatg ccgactgggg gattatggaa aaagcgaaag
     2581 cactaaaaga aacacccccc aatccaacta aagccgccca aatcaaagcc gacggacacg
     2641 ccgatgtcaa aggcagcgat tggggcttcg gctaccaact ggcgtggatg tgggacatca
     2701 acgaccgtgc gcgcgtgggc gtgaactacc gttccaaagt ctcacacacg ctcaaaggcg
     2761 atgccgaatg ggcggcagac gacgcaatgg cgaaacagtt atgggatgca aacaaactcg
     2821 cactgctcgg ctacacgcca agcgaaaaag cccgcgttaa aatcgttacg cccgagtctt
     2881 tgtccgtaca cggtatgtac aaagtgtccg acaaagccga cctgttcggc gacgtaactt
     2941 ggacgcgcca cagccgcttc gataaggcgg aactggtttt tgaaaaagaa aaaaccatcg
     3001 tcaacggcaa atccgaccgc accaccatca cccccaactg gcgcaacacc tacaaagtcg
     3061 gcttcggcgg ttcttatcaa atcagcgaac cgctgcaact gcgcgccggc atcgcttttg
     3121 acaaatcgcc cgtccgcaac gccgactacc gcatgaacag cctgcccgac ggcaaccgca
     3181 tctggttctc cgccggcatg aaataccata tcggcaaaaa ccacgtcgtc gatgccgcct
     3241 acacccacat ccacatcaac gacaccagct accgcacggc gaaggcaagc ggcaacgatg
     3301 tggacagcaa aggcgcgtct tccgcacgtt tcaaaaacca cgccgacatc atcggcctgc
     3361 aatacaccta caaattcaaa taaacggccc gccgtttgat tgtagaaatg ccgtctgaaa
     3421 cagatttccg tttcagacgg cattgtttta ggcgcg
//
LOCUS       DAWKOG010000180         3409 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.180, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000180 DAWKOG010000000
VERSION     DAWKOG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.180"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(236..616)
                     /locus_tag="WJK66_001911"
     CDS             complement(236..616)
                     /locus_tag="WJK66_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HEZ7579565.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDDGGRGHMSSVQNQARPADF
                     RAFSCENGLSVRVRHLDSGKVALRLDGRRAVLSSDVAASGERYTAEHGLFGNATEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            1092..3173
                     /locus_tag="WJK66_001912"
     CDS             1092..3173
                     /locus_tag="WJK66_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEZ7579566.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPALQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEILAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            3163..3357
                     /locus_tag="WJK66_001913"
     CDS             3163..3357
                     /locus_tag="WJK66_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEZ7579567.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
BASE COUNT          744 a         1007 c          876 g          782 t
ORIGIN      
        1 acagcttcga caaagacgcg gttgccaaag agatcgacct acaaagggaa gtaacacagg
       61 cgttcggcaa aacgtcgccc aagtcgcagc ggccgttgcc aacaaacttg gcaatacccg
      121 aagttacgga cggtatcagg tagccaaaac cctgccggag gccgaactgc aaaacaaatc
      181 cgacataaaa aaccgcccga aaaatccgga cggcggttca aacaggctgc cccgtttagc
      241 gggcgcggca ggaagtttcg accgaattgc cgtaggcatc ggtaaagccg aaaaaggctt
      301 cgccgccttt ctggtgccac tcggttgcgt ttccgaacaa accgtgttcg gcggtatagc
      361 gttcgccgga tgcggcaacg tcggaagaga ggacggcacg cctgccgtcc agccgcaacg
      421 cgactttgcc gctgtccaaa tggcggacgc gcacagacaa accgttctcg catgaaaacg
      481 cccgaaaatc tgccgggcgg gcttggtttt gaacggacga catatgccct cgtccgccgt
      541 catcatacgc ctccggcacg gcacaggccg ccaaagacaa aaccggtacg gtcagcgcga
      601 aaaacctgat attcataaaa gctccccaat aaaaataaga tatgaaacaa ccgccctgat
      661 tccaagctgc ggcaacgcca tactataaac ggacgcgcaa acacacaagc ccgataaccg
      721 gaatttaccc gcgatgaatc aataatccgg attgcgcgcc ccttctttac cggtcttccg
      781 gcgccgcctt ttgcctgacg atgccgtcca aacccgcctg tccgcccgaa ggaatgtaaa
      841 ttttttccaa attccaagta aaaaccgcta tcagtgtgct aatttgcgtt aaaatcctat
      901 tcggcgttta acgttttgtg cgcccgcatc cctgcactgt ttgatgcggg cataaggcac
      961 aaatcccgac aagcgcactg tttcatactt cgtcaatcat tcagactccg gtttgtgccc
     1021 gtgccggcag gcggtcatcc gtttcccgcc gttcaggcat attccgacag tgtgagataa
     1081 ggatttattc gatgaaatca ctcaaaacct tcctcatttg gggcatagtg gtactggtcg
     1141 gcttagcatc ctttaccact ctggctctca gccgaggcga acaggtcagc gcggtatgga
     1201 tggttaccgc cgccatatcc gtttactgca tcgcctaccg tttttacagc ctctacatcg
     1261 ccaaccgcgt aatgcggctc gatcctaacc gcctgactcc ggcagaacgc cacaacgacg
     1321 gcttggacta cgttccgacg cacaagggcg tattgttcgg acaccacttt gccgcgattg
     1381 ccggcgcggg ccccttggtc ggcccggttt tggcggcgca aatgggttac ctgcccggta
     1441 ctttgtggat tatcttcggc gtggtgtttg ccggcgcggt acaggatatg atggtcttgt
     1501 tcgtctctat gcgccgcgac ggtaagtctt tgggcgatat tgtgaaacag gaactcggca
     1561 ctgtccccgg cgtgattgcc tccatcggta ttttgatgat tatggtcatc attatggcgg
     1621 tgttggcgtt gattgtcgta aaagcattgg ttcacagccc ttggggtacg ttcaccattg
     1681 ccgcaacgat gccgattgcc ctgtttatgg gtatttacac gcgctatatc cgtccgggca
     1741 aaatcggcga gatttccatc gtcggcttta ttttgctgat gctggcggta atttacggcg
     1801 aagatgtggc gaaaagttcc atcgggcatt ggttcgacct tgacggcatc cagctcactt
     1861 gggcgattat gatttacggc tttgtcgcct ccgtattgcc cgtatggttg ctgctcactc
     1921 cgcgcgacta tctctccacc ttcctgaaaa tcggtacgat tgcggccttg gctttgggca
     1981 tcgtcatcgt caatcccgct ttgcaaatgc ctgccgtaac ccactttatc gacggttcgg
     2041 gtccggtatt ctcaggcgca ttgttcccgt tcctcttcat caccatcgcc tgcggtgcgg
     2101 tttcgggctt ccacgcgctg atttcctccg gcaccacgcc gaaaatgctg gaaaacgaaa
     2161 cccacgtccg tatgattggt tacggcggta tgttgatgga aagtttcgta gccattatgg
     2221 cgcttgccgc cgccgcatcg cttgatcccg gcgtgtactt cgccatgaac agccctgccg
     2281 ccctgatcgg tacggatgcc aataccgccg ccgaagtgat taccaccaag ctgcaattcc
     2341 ctgtcgatgc ggcaaccctg ttgcacaccg ctaaggaagt gggcgaaaac accatccttt
     2401 cccgtgcagg cggtgcgccc accctcgcag tcggtatggc gcacattatg agccgcctga
     2461 ttccgggcga ggcgatgatg gcgttctggt atcacttcgc cctgttgttt gaagccttgt
     2521 tcatcctgac cgccgtcgat gccggtacgc gcgtcgcacg ttttatgatt caagacttgg
     2581 gcagcatctt ctacaaacct ttcggcaaca ccgactccat ccccgccaac ctgattgcga
     2641 ccttcttcgc cgtggcattg tggggctact ttctctacac cggcgtaacc gacccgttgg
     2701 gcggcatcaa ctcgctctgg cctttgttcg gcatcgccaa ccaaatgctg gcaggcgtag
     2761 ccttgattat gtgcgccgtg gtgctgatta agatgaaacg cgaccgttat gtctgggtgg
     2821 tactcgttcc cgccgtcggc gtactgttcg taacctgcta cgccggcctg caaaaactgt
     2881 tccacagcga cccgcgcatc agcttccttg cccacgccgg caaatacagc gacgcattgg
     2941 ctaaaaacga aatccttgcg cctgccaaag acatcggcga aatggcgcaa atcatcttca
     3001 acgacaagat taatgccggt ctgaccatcc tcttcttgtc ggttgtcgtg attgtcgccg
     3061 cgtacggttt gcgtaccgcc ctcaaagcac gcaaagtcgg ctggccgacc gccaaagaaa
     3121 tcccggcggt gtaccgcgac ggcaaacagc cggaggcaca aagtgaagca taagctcgcg
     3181 tcttggtgga aaaccatcaa gctgacggca aacttgatgg caggcgtgcc cgattatgaa
     3241 aactacgttg cacagcagcg caaacataat cccaacgccc ctgtgatgac caagctgcag
     3301 tttcaagact attgccgcaa acgccgctgc ggcgcaaacg gcggacgctg ctgttaagcc
     3361 tgcttgaaac aaattccgtc tgaacgccgc ttcagacgga atttttata
//
LOCUS       DAWKOG010000181         3389 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.181, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000181 DAWKOG010000000
VERSION     DAWKOG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.181"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            36..701
                     /locus_tag="WJK66_001914"
     CDS             36..701
                     /locus_tag="WJK66_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfp pilus assembly protein FimT/FimU"
                     /protein_id="HEZ7579568.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AEQVANLLRFSRGEAVRLNLPVYICPVQVKKGGAPNNKCDFGKNGQGMLAFGDKNGNK
                     TYDGDTADVLLRSVVLNDDINNKRINYAFNHIAFGQTQPTADRVVWTFNQNGTFGYSS
                     DQNLKNNSKFVYSDGYIQIVLTDAKAVSADEKKFRSAVVLINSSGRVEVCPKNDTRAV
                     CRH"
     gene            733..1344
                     /gene="pilV"
                     /locus_tag="WJK66_001915"
     CDS             733..1344
                     /gene="pilV"
                     /locus_tag="WJK66_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEZ7579569.1"
                     /translation="MKNNDCLRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYTGSYTPTSSDGDFKLDNL
                     KSKKDLAKAQLDRFGYELKNALPDAAAIHYAVCKDSSGVAPTLSAGSTFSQNCDGSAN
                     GDTLIKVLWVNDSAGDSDIDRTNLETNGNNIVYTYQARVGGRE"
     gene            1341..2282
                     /locus_tag="WJK66_001916"
     CDS             1341..2282
                     /locus_tag="WJK66_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEZ7579570.1"
                     /translation="MRRKMLNVPKGSYDGMKGFTIIEFLVAGLLSMIVLMAVGSSYFT
                     SRKLNDAANERLAAQQDLRNAATLIVRDARMAGGFGCFNMSEHPATDVVSDVAQKNRL
                     FSLKRNSTNKLIPIAESSNIGYQGFAQARPALIFQYGIDDVNASAETTVVSSCGAISK
                     PGKQIPTLEDAKKELKIQNPDKEQNGNIARQRHVVNAYAVGRIAGEEGLFRFQLDDKG
                     KWGNPQLLAKKIKRMDVRYIYVSSCPEDEDAGKEEQFKYTDKFDSTQNAVTPAGVEVL
                     LDSGLDAKIAASSDNSIYAYRINATIRGGNVCANRTL"
     gene            2261..2860
                     /locus_tag="WJK66_001917"
     CDS             2261..2860
                     /locus_tag="WJK66_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEZ7579571.1"
                     /translation="MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGEFQVLDLEYDTDSKVTFSENCEKGLCTAVNVRT
                     NTNNGNEEAFDNIVVQGKPTVEAVKRSCPANSTNLCIDNKGMEYKKGTQSVSKPPRYI
                     IEYLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            2865..3338
                     /locus_tag="WJK66_001918"
     CDS             2865..3338
                     /locus_tag="WJK66_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEZ7579572.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNTVKQFILKNPLDDNQTIKSKLEIFVSGYKMNPKIAEKYNVSVHFVNKEKPRA
                     YSLVGVPKTGTGYTLSVWMNSVGDGYKCRDAASARAHLETLSSDVGCEAFSNRKK"
BASE COUNT          958 a          680 c          862 g          889 t
ORIGIN      
        1 aatttaatcc acgatacatt accagttaac gtcctatgtg tacacgaaaa caacaaggtt
       61 tcacgctaac agagctgctc atcgtgatgg tcattgcagc cattatggcg atgatagccc
      121 tccccaatat gagccaatgg attgcatccc gccgcattgc cagtcacgcg gagcaggttg
      181 ccaacctttt gcgtttctcc aggggagaag ccgtccggct caatcttcct gtctatatct
      241 gtcctgttca agttaaaaaa ggcggtgcgc ccaacaataa atgtgacttc ggcaagaatg
      301 ggcagggaat gttggctttt ggcgataaga atggcaataa gacatatgac ggcgatacgg
      361 cggatgttct tctccgcagt gtggtattga atgatgatat caataataag cggattaatt
      421 atgccttcaa ccatatcgct ttcggtcaga ctcagccgac cgctgaccgt gtagtttgga
      481 cattcaacca aaacgggaca ttcggctatt cgtccgatca gaatctcaag aataattcca
      541 aatttgttta ttctgacggt tatatccaaa tcgtgttgac agatgcgaag gcggtttctg
      601 ccgatgaaaa gaaattccgt tcggcggtgg ttttgattaa cagcagcggc agggttgaag
      661 tttgtcctaa aaacgatacg cgcgccgtat gccgacatta atgattgttt tagtcggttt
      721 gggaaataaa aaatgaagaa taatgattgc ttgcgcctga aaaatcccca gtccggtatg
      781 gcgctgatag aagtcttggt tgctatgctc gttctgacca tcggtatttt ggcgttactg
      841 tctgtgcagt tgcggacagt cgcttccgtc agggaggcgg agacacaaac catcgtcagc
      901 caaatcacgc aaaacctgat ggaaggaatg ttgatgaatc cgaccattga ttcggacagc
      961 aacaagaaaa actataatct ttacactggg tcgtacaccc ccacttcctc tgacggcgat
     1021 ttcaagcttg ataatttgaa aagcaagaag gatttggcaa aggcccagtt ggacaggttc
     1081 ggttatgaat tgaaaaatgc cttgccggat gcggcagcca tccattacgc cgtctgcaag
     1141 gattcgtcgg gtgttgcgcc gacattgtcc gccggcagta ctttttctca aaattgcgat
     1201 ggtagtgcaa atggggatac tttgattaaa gtattgtggg taaatgattc ggcaggggat
     1261 tcggatatcg accgtacgaa tcttgagacg aacggcaaca atatcgtata tacctatcag
     1321 gcaagggtcg gaggtcggga atgagacgta aaatgctaaa cgtaccaaaa ggcagttatg
     1381 atggtatgaa aggttttacc attattgaat ttttggttgc gggcctgctc agtatgattg
     1441 tcctgatggc ggtcggatcg agttacttca catcccggaa attaaatgat gcggcaaacg
     1501 agcgtcttgc cgcacaacag gatttgcgga atgcggcaac attgattgtc cgcgatgcaa
     1561 gaatggcagg gggcttcggt tgtttcaata tgtccgagca tcctgcaact gatgttgttt
     1621 ccgatgtggc gcaaaaaaac cgtctttttt ccttaaaaag gaacagtaca aataaactta
     1681 tccccatagc ggaatcttca aatatcggat atcagggttt tgcccaggct cgtccggcat
     1741 tgattttcca atacggaatc gatgatgtta atgcaagcgc cgagactacc gtcgtcagca
     1801 gctgtggcgc aatatcgaaa ccgggtaagc aaatccctac tttagaagat gcaaaaaaag
     1861 aattgaagat tcagaatcct gataaggagc aaaatggcaa tatagcgcgt caaaggcatg
     1921 tggtcaatgc ctatgcggtc ggcaggattg ccggtgagga aggtttgttc cgcttccaat
     1981 tggatgataa gggcaagtgg ggtaatcctc agttgctcgc gaaaaagatt aaacgtatgg
     2041 atgtgcggta tatctatgtt tccagttgtc ctgaagatga agatgccggg aaagaggaac
     2101 aattcaaata tacggataaa ttcgacagca cccaaaatgc tgttacgcct gccggggtgg
     2161 aggttttatt ggatagcggc cttgatgcca agattgccgc ttcttcagac aatagtattt
     2221 atgcttaccg tatcaatgcg acaatacgcg ggggaaatgt atgcgcaaac agaacacttt
     2281 gacgggaatc ccgacttctg acggacagag ggggtttgca ctgtttatcg tactgatggt
     2341 gatgatcgtc gtggcatttt tggttgtaac tgccgcgcag tcttacaata ccgagcagcg
     2401 gatcagtgcc aacgaatcag acaggaaatt ggctttgtct ttggccgagg cggctttgcg
     2461 ggagggcgaa tttcaggttt tggatttgga atatgatacg gacagtaagg ttacatttag
     2521 tgaaaactgt gaaaaaggtc tgtgtaccgc agtgaatgtg cggacaaata caaataatgg
     2581 taatgaagag gcttttgaca atatcgtggt gcaaggcaag cccaccgttg aggcggtgaa
     2641 gcgttcttgc cctgcaaatt ctaccaacct gtgcattgac aataaaggga tggaatataa
     2701 gaaaggcacg caaagtgtca gcaaaccacc gcgctatatt atcgaatatt tgggcgtgaa
     2761 gaacggagaa aatgtttatc gggttactgc caaggcttgg ggtaagaatg ccaataccgt
     2821 ggtcgtcctt caatcttatg taagcaataa tgatgagtaa taaaatggaa caaaaagggt
     2881 ttacattgat tgagatgatg atagtcgtcg cgatactcgg catcatcagc gtcattgcca
     2941 taccttctta tcaaagttat attgaaaaag gctatcagtc ccagctttat acggagatgg
     3001 tcggtatcaa caatactgtc aaacagttta ttttgaaaaa tcccctggac gataatcaga
     3061 ccatcaagag caaactggaa atatttgtct caggctataa gatgaatccg aaaattgccg
     3121 aaaaatataa tgtttcggtg cattttgtca ataaggaaaa accaagggca tacagcttgg
     3181 tcggtgttcc aaagacgggg acgggttata ctttgtcggt atggatgaac agcgtgggcg
     3241 acggatacaa atgccgtgat gccgcttctg cccgagctca tttggagacc ttgtcctcag
     3301 atgtcggctg tgaagccttc tctaatcgta aaaaataggg ctgttttgcc aatgccgtct
     3361 gaagattaat tttcagacgg tatttttat
//
LOCUS       DAWKOG010000182         3378 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.182, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000182 DAWKOG010000000
VERSION     DAWKOG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.182"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(214..1278)
                     /locus_tag="WJK66_001919"
     CDS             complement(214..1278)
                     /locus_tag="WJK66_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ7579573.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLCALTRFIRENRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIIH
                     NGIDTDRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGH
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTENIASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIVEHHQSGILLDRLTPESLADEIERLVLNPEAKNALATAAH
                     QCVAARFTINHTADKLLDAI"
     gene            complement(1279..2037)
                     /locus_tag="WJK66_001920"
     CDS             complement(1279..2037)
                     /locus_tag="WJK66_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ7579574.1"
                     /translation="MKKVSVLIVAKNEANHIRECIESCRFDKEVIVIDDHSTDNTAEI
                     AEGLGAKVFQRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            complement(2112..2187)
                     /locus_tag="WJK66_001921"
     tRNA            complement(2112..2187)
                     /locus_tag="WJK66_001921"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2152..2154),aa:Ile,seq:cat)
     gene            complement(2241..3378)
                     /locus_tag="WJK66_001922"
                     /pseudo
     CDS             complement(2241..3378)
                     /locus_tag="WJK66_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247614.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
BASE COUNT          765 a          822 c          899 g          892 t
ORIGIN      
        1 tctactatac attgtaacaa agcacttgat gcggcagcac aagccgggga agacgctgca
       61 aaactttgcc aataggctgc gtaatatcgg gggatattgg gattttctta tttttaagga
      121 tggattcggg gtgtttcttt ccaatgaacg aatataattt ggcggtttgt tgcatcaaag
      181 gctttcagac ggcatatgcc gtctgaaagc agtttatatt gcatccaata atttgtcggc
      241 ggtatggttg atggtaaaac gggcggcgac gcattgatga gctgccgttg ccagtgcgtt
      301 ttttgcttcg gggtttaaga cgaggcgttc gatttcgtcc gccaaagatt caggcgtaag
      361 cctatccagc agaatccccg attgatgatg ttcgacaatt tccttttgcg cccccaaagt
      421 attggaaatc accgccgttc ggcagtacat cgcctcgcat aatgacaaac cgaatgcctc
      481 cgggacgagg ctgggcaaaa ccacgacatc gctttggcgg taaaacgaag caatgttttc
      541 ggtaaaccct tcaaaagaaa caaatggttc tgctcctgaa gcagatacgt cccgcttcag
      601 gcggcacata taatcaggat gtccgtgccc tgccaatttg agtctgattt gaggatattt
      661 ccgatgcaat atcacacagg cttcaattag gttttccaat cctttttccg gactgatcct
      721 gccggcgtag gcgacggtaa aaaaacggct gtcgggtttt tcttgagagg gagggaaccg
      781 atcggtatcg ataccgttat gaataatccg gtatttttct ttaaagggat tgtcggcggt
      841 ttgcacatcg taaaccagac gggaaacgca aataaagcgg tctgtgtttt tctgtatcag
      901 gcggtggtaa aaatcggttt tgttggcgac gacattatgt ttgacaaata tcaggcgcac
      961 cccggtcagt tttttcaaaa gtatggataa ggcggcaatt ttgccggtgt gtatcatcag
     1021 gtgggaaatg cggttttccc ggataaagcg ggtaagcgcg cagagcgaaa acagcccgtt
     1081 gagcgtgtga aggcgtgtcg tgaaaacgga agaaacttcg gaaaagcgtc tgtgcatcaa
     1141 ctcattattt ttattgacgg cggtaaacat tgtgcagccc cgaagcccca atgcttttga
     1201 aacatcatag acatattgtt cgcccccacc ccaaatcttg gccgatacga tatttaatat
     1261 cctgaattct ttttccattt aaaattttcc gccagatttg tacagataat atagtttgac
     1321 atatttaatc atcgtgtaat agctgtggtt gacggacata atccaaccca tttttccatc
     1381 aagaaaccct ttgttcagaa tataaatttt gaaaaaaccc caaatcggac ggaggataat
     1441 gtccctaacg aaacgcacgg gctttccctg ctctcggtat ttctcggctg agatggaagt
     1501 atatttgttg aacttgttga aatattgttc ccagttgtcg tacgtataat ggtacataaa
     1561 atgtttcaga cggcgtttgg ggtagggggt ttgtacggtt tcgtgtactt tgccttgcac
     1621 cgaactgtct ttcttcggca tcaaacggca tacgctgtcg ggacgcatcg cgccgtgtgt
     1681 ggcgggatgg ttggggaaaa ggttgcggcg ctcgacaaaa taggcggcat aatcgccggt
     1741 ttggacaatt tttgagattt catcagatag ttccggcgtg cagcgttcgt ctgcatcaat
     1801 caggaaaacc cattctccgc ctgcctgttc gatggcaaat gttttttgcg ctccgaaatc
     1861 cccgttcaaa tgccgttgga agacttttgc acccaaaccc tcggcaattt cggcagtatt
     1921 gtcggtgctg tggtcgtcga taacgataac ttctttatcg aaacggcaac tttcaataca
     1981 ttcccgaatg tggtttgctt cgtttttggc aacaatcaat acgctgactt ttttcattat
     2041 caaggtttct caaagcattt tggtttcaca gggcggcaaa aaagcccaaa catacgtccg
     2101 ggcttgggaa ttggtgggtc cggtggggtt cgaacccacg accaagggat tatgagtccc
     2161 ctgctctaac ccctgagcta cagacccgtc aagagtaggc gcgtattgta tagcatagcg
     2221 gtgggattga caaacttatt tcatccgatg attaagacgc agttgcttcc cccgaaggca
     2281 aacgatgagc tggcggcaat gcgtttttcg gtttcccagc ggctgccgct gccggtcagg
     2341 ttgatggcgg gcaggttggg gtcgttctgc ccgtcccaaa gccggggcgg aagttttcct
     2401 tcgggattgc tttgccggtt ggcaatgccc cacgcgaacg cggcttcgat tgcgtccgcc
     2461 gcgcccagcg tgtgtccggt ttgcggcttg gtggacgtgc agggcgtatt gttgccgaaa
     2521 accgctgcaa cggcgcggct ttccatattg tcgttgtggt gcgtcccggt gccgtgcaga
     2581 ttaatccagc cgatgtcttc gggcgcaaga cctgcgtgtt gcaatgccgt ctgaaaggca
     2641 aggattgcgc cttacgcgtc ggggcggggc gtggaaatat ggtaggcatc gctgttggct
     2701 ccgtagccga cgagttcggc gtaaattttc gcaccgcgcc gtttggcgtg ttccaattct
     2761 tccagcacga aaatccccgc gccttcgccg atgaccagcc cgtcgcggtt cgcgtcgtat
     2821 gggcgcgggg ttttttccgg ttcgccgttg cggcggctgg cggcataaag cgagtcgaaa
     2881 acatacactt cggacgggaa aaattcttcg cctccgcccg ccagcatcat atcggtcaga
     2941 ccgtatttga tggcttcgta ggcataacct atgccttggc tgccggacga acacgcgctc
     3001 gatgtcggga tgatgcgtcc tttcagcccg aaaaagatgc cgatattggc ggcggcggtg
     3061 tgcggcatca tacgcacata ggtgttggca ctgaagttgc gcgacatgcc ggtcagcaac
     3121 aattcgccta catcgccgat gtctttggtg ctgccgctgg aagagccgca ggcaacgccc
     3181 atgcgtccgt cagtaatgct ttcgtctccg agcaaaccgg catccgtcaa agcctgctcc
     3241 gccgcatcga cgcacaaata cgacacacgc cccatacttc tgagctgctt gcgcgtccaa
     3301 tgtttcggcg gcgcgtaatt ttcaatcggc gcacccagtt gcgcttccaa ttcggggaaa
     3361 cgttcgtgcc aatccact
//
LOCUS       DAWKOG010000183         3364 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.183, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000183 DAWKOG010000000
VERSION     DAWKOG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.183"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     regulatory      65..168
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            235..1458
                     /gene="cytX"
                     /locus_tag="WJK66_001923"
     CDS             235..1458
                     /gene="cytX"
                     /locus_tag="WJK66_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HEZ7579575.1"
                     /translation="MSGNASSPSSSAAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRQARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGASANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVLV
                     RLFFKKTQSLQRNPS"
     gene            1455..2555
                     /locus_tag="WJK66_001924"
     CDS             1455..2555
                     /locus_tag="WJK66_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ7579576.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVVRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWHADDIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPEDLQAQYDWLIDCRGYGAKTAWNASAEHGSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYSRKRRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIRRQD"
     gene            2576..3193
                     /gene="thiE"
                     /locus_tag="WJK66_001925"
     CDS             2576..3193
                     /gene="thiE"
                     /locus_tag="WJK66_001925"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEZ7579577.1"
                     /translation="MTFPPLKSPLKFYAVVPTADWVGRMVKAGADTVQLRCKTLHGDE
                     LKREIARCVAACQGSRTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYVASGAIFPTTTKQMPTAPQGLDKLREYVKQAGGTPVVA
                     IGGIDLNNAEEVLATGVSSLAVVRAVTEAANPEAVVKAFQALWDG"
BASE COUNT          677 a         1048 c          938 g          701 t
ORIGIN      
        1 aatgcgccgt gccgcctgaa atgtaagatt tttgccaacg ccccctgctt ttgtgtacac
       61 ttaaagctcc ttgtcggagt gccgccgccg ggcggctgag attgcgaaag cagaatccgt
      121 agaacctgtc ggggtaatgc ctgcgtagga aacaaaccgt caaatgcctt atcaggcttc
      181 cgttcccttt tccgcacttc cccgcccacc ttccttcttt ttaaaggacg tcatatgtcg
      241 ggcaatgcct cctctccttc atcttccgcc gccatcgggc tgatttggtt cggcgcggcg
      301 gtctcgattg ccgaaatcag cacgggtacg ctgcttgcgc ctttgggctg gcaacgcggt
      361 ctggcggctc tacttttggg tcatgccgtc ggcggcgcgc tgttttttgc ggcggcgtat
      421 atcggcgcac tgaccggacg cagctcgatg gaaagcgtgc gcctgtcgtt cggcaaacgc
      481 ggttcagtgc tgttttccgt ggcgaatatg ctgcaactgg ccggctggac ggcggtgatg
      541 atttacgccg gcgcaacggt cagctccgct ttgggcaaag tgttgtggga cggcgaatct
      601 tttgtctggt gggcattggc aaacggcgcg ctgattgtgc tgtggctggt tttcggcgca
      661 cgcaaaacag gcgggctgaa aaccgtttcg atgctgctga tgctgttggc ggttctgtgg
      721 ctgagtgccg aagtcttttc cacggcaggc agcaccgccg cacaggtttc agacggcatg
      781 agtttcggaa cggcagtcga attgtccgcc gtcatgccgc tttcctggct gccgctggcc
      841 gccgactaca cacgccaagc acgccgcccg tttgcggcaa ccctgacggc aacgctcgcc
      901 tatacgctga cgggctgctg gatgtatgcc ttgggtttgg cagcggcgtt gttcaccgga
      961 gaaaccgacg tggcaaaaat cctgctgggc gcaggtttgg gtgcggcagg cattttggcg
     1021 gtcgtcctgt cgaccgttac caccactttt ctcgatgcgt actccgccgg cgcgagtgcg
     1081 aacaatattt ccgcgcgttt tgcggaaaca cccgtcgctg tcggcgttac cctgatcggc
     1141 acggtacttg ccgtcatgct gcccgttacc gaatatgaaa acttcctgct gcttatcggc
     1201 tcggtatttg cgccgatggc ggcggttttg attgccgact ttttcgtctt gaaacggcgt
     1261 gaggagattg aaggctttga ctttgccgga ctggttctgt ggcttgcggg cttcatcctc
     1321 taccgcttcc tgctctcgtc cggctgggaa agcagcatcg gtctgaccgc ccccgtaatg
     1381 tctgccgttg ccattgccac cgtactggtg cgccttttct ttaaaaaaac ccaatcttta
     1441 caaaggaacc cgtcatgacc cgtatcgcca tcctcggcgg cggcctctcg ggaaggctga
     1501 ccgcactgca gcttgcagaa caaggttatc agattgcact tttcgataaa ggctgccgcc
     1561 ggggcgaaca cgccgccgcc tatgttgccg ccgccatgct cgcgcctgcg gcggaagcgg
     1621 tcgaagccac gcccgaagtg gtcaggctgg gcaggcagag catcccgctt tggcgcggca
     1681 tccgatgccg tctgaacacg cacacgatga tgcaggaaaa cggcagcctg attgtgtggc
     1741 acgggcagga caagccatta tccagcgagt tcgtccgcca tctcaaacgc ggcggcgtag
     1801 cggatgacga aatcgtccgt tggcacgccg acgacatcgc cgaacgcgaa ccgcaactcg
     1861 gcggacgttt ttcagacggc atctacctgc cgaccgaagg ccagctcgac gggcggcaaa
     1921 tattgtctgc acttgccgac gctttggacg aactgaacgt cccttgccat tgggaacacg
     1981 aatgtgcccc cgaagacctg caagcccaat acgactggct gatcgactgc cgcggctacg
     2041 gcgcaaaaac cgcgtggaat gcttctgccg aacacggcag caccctgcgc ggcatacgcg
     2101 gcgaagtggc gcgggtttac acacccgaaa tcacgctcaa ccgccccgtg cgcctgctgc
     2161 acccgcgtta tccgctctac atcgccccga aagaaaacca cgtcttcgtc atcggcgcga
     2221 cccaaatcga aagcgaaagc caagcccccg ccagcgtgcg ttcagggttg gaactcttgt
     2281 ccgcactcta tgccgtccac cccgccttcg gcgaagccga catcctcgaa atcgccaccg
     2341 gcctgcgccc cacgctcaac caccacaacc ccgaaatccg ctacagccgc aaacgccgcc
     2401 tcatcgaaat caacggcctt ttccgccacg gtttcatgat ctcccccgcc gtaaccgccg
     2461 ccgccgtcag attggcagtg gcactgtttg acggaaaaga cgcgcccgaa cgtgatgaag
     2521 aaagcggttt ggcgtatatc cgaagacaag attaaagccg tctgaaagga accccatgac
     2581 cttcccgcca ctcaaatccc cgctcaaatt ctacgccgtc gtccccaccg ccgattgggt
     2641 ggggcgcatg gtcaaagcag gtgccgacac ggtgcaactg cgctgcaaga ccctgcacgg
     2701 cgatgaattg aaacgcgaaa tcgcccgctg cgtcgccgcc tgtcaaggca gccgtacgca
     2761 gcttttcatc aacgaccact ggcgcgaggc aatcgaagca ggcgcgtacg gcgtgcatct
     2821 cggacaagaa gacatggaca ccgccgacct tgccgccatc gccgccgccg gtttgcgctt
     2881 gggtttgagt acgcactccg ttgccgaact cgaccgcgcc ctgtccgtac accctagcta
     2941 cgtcgccagc ggcgcgattt tcccgaccac gaccaagcaa atgcccaccg ccccgcaagg
     3001 cttggacaaa ctgcgcgaat atgtgaaaca ggcaggcggc acgcccgtcg tcgccattgg
     3061 cggcatcgat ttgaacaatg ctgaagaggt attggcaacc ggcgtttcct cactcgccgt
     3121 cgtccgcgcc gtgaccgaag cggcaaatcc cgaagcggtg gttaaagcgt ttcaggcttt
     3181 gtgggatgga taaaaccgaa agaagaaaat tcaattgccg tgtaggcaaa acttagcccg
     3241 ttatcgcaaa catacttaac tttaaatgtg gcatatcatc aaattccgtc atttccgcgc
     3301 aagcgggaat ccgccttaaa acttgagaaa ccatcatttg aaaaacagtt tccgaatttc
     3361 aaaa
//
LOCUS       DAWKOG010000184         3319 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.184, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000184 DAWKOG010000000
VERSION     DAWKOG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.184"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(159..2291)
                     /locus_tag="WJK66_001926"
     CDS             complement(159..2291)
                     /locus_tag="WJK66_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEZ7579578.1"
                     /translation="MSIVSAPLPALSALIILAHYHGIAANPADIQHEFCTSAQSDLNE
                     TQWLLAAKSLGLKAKVVRQPIKRLAMATLPALVWCDDGNHFILAKTDGGGEHAQYLIQ
                     DLTTNKSAVLSFAEFSNRYSGKLILVASRASVLGSLAKFDFTWFIPAVIKYRRLFFEV
                     LVVSVVLQLFALITPLFFQVVMDKVLVHRGFSTLDVVSVALLVVSLFEIVLGGLRTYL
                     FAHTTSRIDVELGARLFRHLLSLPLSYFEHRRVGDTVARVRELEQIRNFLTGQALTSV
                     LDLAFSFIFLAVMWYYSSTLTWVVLASLPAYAFWSAFISPILRTRLNDKFARNADNQS
                     FLVESITAVGTVKAMAVEPQMTQRWDNQLAAYVASGFRVTKLAVVGQQGVQLIQKLVT
                     VATLWIGARLVIEGKLTVGQLIAFNMLSGQVAAPVIRLAQLWQDFQQVGISVARLGDI
                     LNAPTENASSHLALPDIRGEITFEHVDFRYKADGRLILQDLNLRIRAGEVLGIVGRSG
                     SGKSTLTKLVQRLYVPEQGRVLVDGNDLALAAPAWLRRQVGVVLQENVLLNRSIRDNI
                     ALTDTGMPLERIIEAAKLAGAHEFIMELPEGYGTVVGEQGAGLSGGQRQRIAIARALI
                     TNPRILIFDEATSALDYESERAIMQNMQAICANRTVLIIAHRLSTVKTAHRIIAMDKG
                     RIVEAGTQQELLAKPNGYYRYLYDLQNG"
     gene            complement(3187..>3319)
                     /locus_tag="WJK66_001927"
     CDS             complement(3187..>3319)
                     /locus_tag="WJK66_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ7579579.1"
                     /translation="CDKLDVSGFIHYRINHSKEFADRQNHINGIENFWNQAKRVLRK"
BASE COUNT          949 a          892 c          742 g          736 t
ORIGIN      
        1 gatgagtgaa aaagaagtgc aggctgccta aaaagacaga aaaagtcttt caggcagcct
       61 gcactttggt ttcatttcag tcagtaaacc cagtaaacga cggtctgaaa acgcagaacg
      121 ttacgaaaaa agcagcctac acgcccatcc cccgccttct acccgttctg taaatcatac
      181 agatagcggt aatatccgtt cggcttcgcc agcaattcct gctgtgttcc cgcttccaca
      241 atcctgcctt tatccatggc aatgatccgg tgtgccgttt taacggtgga cagacggtgg
      301 gcgataatca gcaccgtccg gttggcgcaa atggcctgca tgttctgcat aatcgctcgt
      361 tcactttcat aatccagcgc gctggtggct tcgtcaaaaa tcagaatgcg cggattggtg
      421 attaacgcgc gggcaatcgc aatacgctgc cgctgtccgc ccgacaagcc ggccccttgt
      481 tcgcccacca cggtgccgta gccttccggc agctccataa taaactcgtg tgcgcccgcc
      541 agtttggctg cttcgataat gcgttccagc ggcatacccg tatccgtcag cgcgatattg
      601 tcgcgtatgc tgcggttgag cagcacattc tcctgcaaga ccacgccgac ctgccgccgc
      661 agccaggcag gagcggccaa agccaaatcg ttgccgtcca ccaacacccg tccctgctcc
      721 ggtacataca gacgctgcac caatttggtg agtgtggatt tgcccgaccc cgaacgtccc
      781 acaatcccca gcacttcccc cgcccgaatc cgcaggttca aatcctgcaa aatcagcctg
      841 ccgtccgcct tatagcggaa atcgacatgt tcgaacgtaa tctccccccg gatatcgggc
      901 aaagccaaat gcgaagacgc attctcggtc ggcgcattca gaatatcccc caaacgcgcc
      961 accgaaatcc ccacctgctg gaaatcctgc cacaactgcg ccaaacggat aacaggcgcc
     1021 gccacctgtc ccgagagcat attaaacgca atcagctgcc ccaccgtcag cttgccctca
     1081 attaccagcc gtgcgccaat ccacaacgtc gccaccgtca ccagcttctg aatcagctgc
     1141 accccctgct ggccgaccac cgccaacttc gttacccgaa atcccgaagc cacataagcc
     1201 gccaactgat tgtcccaacg ctgcgtcatc tgcggctcca ccgccatcgc ctttaccgta
     1261 cccaccgcag tgatgctttc tactaaaaac gactggttgt ctgcattgcg cgcgaactta
     1321 tcgttcagac gcgtccgcag tatcggactg ataaatgccg accaaaacgc ataggcaggc
     1381 aacgaagcca ataccaccca agtcagagtg gagctgtaat accacatcac cgccagaaag
     1441 ataaacgaaa acgccaaatc caacaccgaa gtcagcgcct gaccggtcaa gaaattgcga
     1501 atctgctcca attcccgcac ccgagccacc gtatcaccca ctcgtctgtg ctcgaaatag
     1561 gataaaggca gggaaagcag atgccggaac aaacgcgcgc ccaattccac atcaatacgt
     1621 gaagtcgtat gtgcaaacag atacgtccgc aaaccgccca acacaatctc aaacagcgac
     1681 accaccaaca aagccaccga caccacatcc aaagtagaga atccccgatg taccagcacc
     1741 ttgtccatca ccacttggaa aaacagaggc gtaatcagcg caaacagctg caacaccacc
     1801 gacaccacca atacttcaaa aaacaaccgg cggtatttga ttaccgccgg aataaaccag
     1861 gtaaagtcaa actttgccaa actgcccaat accgaagcgc gggaagcaac caatatcagt
     1921 ttgcccgaat atctgttaga aaattcggca aaagacaata ccgcagactt attcgtagtt
     1981 aaatcctgta ttagatattg ggcatgctca cccccaccgt ctgttttagc caaaataaaa
     2041 tggttgccgt catcacacca taccaatgcg ggtaaagtcg ccatagccaa acgtttaata
     2101 ggctggcgga ctacctttgc cttcaatccc aaagatttgg cggctaacag ccattgcgtt
     2161 tcatttaaat cgctctgtgc ggaagtacaa aattcatgct gtatatcggc aggattggcg
     2221 gcaatgccgt ggtaatgggc gaggatgatg agggcggaaa gggcggggag cggtgcggat
     2281 acgatagaca taaataaaat atagttagat tggatgtgga taacggctgg ctggaaaagg
     2341 aatatattaa gtagaaagaa atatataaat aaaacagcag aacgcattgt aaggatatat
     2401 atgggaattg taaagagaaa gtatggaaaa gttctcgttt caggaaggta aaacggctta
     2461 ggaatcgagt tagatgagga tgcctcgcac ctctcgtgcc tcctgcatac cgttaaggaa
     2521 cagggttaag gtgcaggctg ctccgaactc tgttgcggtc ggataatgtt attttttgtg
     2581 tttcaggcag cctgaaatat ctgtatattt ttgttttaaa tagattttaa agattgataa
     2641 ctgttcttga cgatttttca agaaaggagt aaagtgactt attatcaatg acaagcaacg
     2701 cgcgaagtga caaggaaaac tatctactta aattctaagg aggcttcgaa tatcataaac
     2761 caatcagaaa catagagata aaaattatgt acaaatataa tcctcttata caatttattg
     2821 cacagttgat tatgtcttat ggagcaagcg tagggtgggc acttgctgcc cacgcgtttc
     2881 atatttcaag gcagcctgaa actgtgtggg cataatgcct accctacatc ccaaaaaaac
     2941 aagcgcagcc tgcgtgtgta gggtgcgaac tttcggcagg cagacacgcg gttttatagt
     3001 ttcaagctga gttgggatgc ttaagataaa gtacaaaacc ttaaaaaata agggggctgt
     3061 actagattag ccctaaattc cacaccaatc ccgcaaaatt tttagctgtc gggacggtgt
     3121 gccgaagtta aatcgaaatt cgcattcttt caagaacagc gggaaagatt tgcgatcaat
     3181 tccgttctat ttgcgcaaga cgcgttttgc ctgattccaa aagttctcaa tgccgttaat
     3241 gtggttttga cggtctgcaa attccttgga atggttgatg cggtaatgga taaaaccgct
     3301 cacgtccaac ttgtcgcag
//
LOCUS       DAWKOG010000185         3277 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.185, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000185 DAWKOG010000000
VERSION     DAWKOG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.185"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(699..1283)
                     /gene="rnhB"
                     /locus_tag="WJK66_001928"
     CDS             complement(699..1283)
                     /gene="rnhB"
                     /locus_tag="WJK66_001928"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEZ7579580.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDALAEMIKNQAVAWHVAAAGPEEIASLNILHATMLAMKRAVDGLAVRPEKIFIDG
                     NRIPEHLNIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALEKYGVLPEHRRDFAPVRNLLAQQALF"
     gene            complement(1352..3247)
                     /gene="mnmG"
                     /locus_tag="WJK66_001929"
     CDS             complement(1352..3247)
                     /gene="mnmG"
                     /locus_tag="WJK66_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEZ7579581.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGERISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTYEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIEGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVRGQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAVEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSENLSAEVIEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDGIDYGKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
BASE COUNT          585 a          787 c         1002 g          903 t
ORIGIN      
        1 cattcccgca aaaacaaaaa aaatcaaaaa cacaaaactg aaatatcgtc attcccgcgc
       61 aggcgggaat ctaggtctgt cggtacggaa acttatcggg aaaaacggtt tttccaaacc
      121 tgagactccg gattcctgtt ttcgcgggaa tccggttttt tgagtttcag tcatttttga
      181 taaattcttg cagctttgag tttctagatt cccgcttttg cgggaatgac gcggaaaagt
      241 tgctgtgatt tcggataaat tttcgtcacg cttaatttct gttttatccg ataaatgcct
      301 gcaatctaaa atttcgtcat tcccgcgaag gcgggaatct aggtctgtcg gtacggaaac
      361 ttatcgggta aaacggtttt gccagccctg agactccgga ttcctgtttt cgtaggaatc
      421 cggttttttg agcttcagtc atttttgata aattcttgca gctttgagtt tctagattcc
      481 cgcttttgcg ggaatggcgg tttggaagtt acctgaaatt caaaaaaaaa aaaacggaaa
      541 ccggacggat tggattcccg cctgcgcggg aatgacggat tttaggtttt tttttgattt
      601 tctatttttc gcgggaatga cggtttgggt tctttctctt tggagttgcg atgccggaaa
      661 tgccgtctga aggcttcaga cggcattttt gtgccggttt aaaacaaggc ctgctgcgcg
      721 agcaggtttc tgacgggggc gaagtcgcgg cggtgttcgg gcagcacgcc gtatttttcg
      781 agggcttcca aatgctgctt cgtgccgtaa cctttgtgtt tgtcgaaacc gtattgggga
      841 tggcgttgcg ccagtgcgta catttccgca tcgcgggcgg tctttgccaa aacggatgcg
      901 gcggatattt cgatgatttt gctgtcgcct ttgacgacgg cttcggcagg gatgttcaaa
      961 tgttcgggaa tgcggttgcc gtcgatgaat attttttcgg gacgcacagc caaaccgtca
     1021 acggcgcgtt tcatcgcgag catggtggcg tgcaggatgt tgaggctggc gatttcttcg
     1081 ggcccggcgg cggcgacgtg ccacgcaacc gcctgatttt ttatcatttc ggcaagcgcg
     1141 tcgcgttttt tctcgctgag ttttttggag tcggtcagtc cgggcaggtc gaatgtttcc
     1201 ggaaggatga cggcggcggc aaacacgctg ccgactaaag gtccgcgtcc tgcctcgtcc
     1261 acgccggcgg tcagtatgtg catgatgttt cctgtcggga tggtgggaat gccgtctgaa
     1321 cggtgtttca gacggcatcg cgccgatgtg tttatttcgc gtctttaaat ccgcgcttca
     1381 aatgcaccat cagcagtgcg acggcggcag gcgttacacc ggaaatgcgg cttgcctgcc
     1441 cgacggtttc gggtttgtgc tggttgagct tttgctgcac ttctgccgac aagcctttga
     1501 ctttgccgta atcgatgccg tcgggcagtt ttaaggtttc gatgtcgcgg cggctgtcga
     1561 tttcttcgtt ttggcggtcg atatagcctt ggtatttgac ttggatttcg acttgttcga
     1621 tgacttcggc ggagaggttt tcagacggca tcgcgccttc gagcgtcatc agcgcggcgt
     1681 agtcgaggtt cgggcggcgt aacaaatcgt gcaggttggc ttctcggctg agtttttgtc
     1741 cgaatacgcg gatttgttcg tcttcggcga gtttttgcgg tgtgtaccac gttgttttca
     1801 aacgttggat ttcgcgttcg acggcttcgc gtttttcgtt gaacatacgc cattgcgctt
     1861 cacccaccaa gccgattttg tagccgtctt cggtcaggcg catgtcggcg ttgtcttccc
     1921 tgagttgcag gcggtattcg gcgcggctgg tgaacattcg gtagggttcg ttcacgcctt
     1981 tggtgatgag gtcgtccacc aatacgccga ggtaggcttg ttcgcggcgc agcaggagcg
     2041 ggtcttgtcc gcgcacatat tgcacggcgt tcgcgcctgc caataaacct tgcgcggcgg
     2101 cttcttcgta gccggtcgta ccgttgattt gcccagcgaa aaacagtccc tcaatggttt
     2161 tggtttcgag gctggctttg aggttgcgcg ggtcgaagta gtcgtattcg atggcgtagc
     2221 cggggcgcag gatatgggcg ttttccaaac ctttcatact gcggacgagc gcgatttgga
     2281 tgtcgaacgg caggctggtg gagataccgt taggatagta ttcgtacgta gtcaggcctt
     2341 cgggttctag gaaaatctgg tggctgtctt tatcggcgaa acggttgatt ttgtcttcga
     2401 tagacggaca ataacgcggg cccacgcctt cgattttgcc ggtaaacatc gggctgcggt
     2461 cgaagcctga gcggatgatg tcgtgggttt gcgtgttggt atgcgtaatc cagcaggaca
     2521 cttggcgcgg gtgcatttcg gcgttgccgc gcacggacat gacgggaacg ggtgtgtcgc
     2581 cgggctgttc ggtcagttgg gaaaagtcaa tcgtgcgtcc gtcgatgcgc ggcggcgtgc
     2641 cggttttcag acggccttgc ggcagcttca attcgcgcaa acgtccgccc agcgatttgg
     2701 cggcggggtc gccggcgcgg ccgccttcgt agttttccaa accgatatgg attttgccgg
     2761 acaaaaatgt gcctgcggtc agcaccacgg cgcgggcttt gaattccacg cccatcgcgg
     2821 tgattacgcc gctgatgcgt tcgccgtcga gcgttacgtc ttcgacggct tgttggaaaa
     2881 ggtcgaggtt ttcttggttt tccaacattt cgcggatggc ggctttgtac aaaatgcggt
     2941 cggcctgcgc gcgcgtggct cgaactgccg cgcctttgct ggcgttcagg cggcggaact
     3001 ggataccgga tttgtcggtg gccaacgcca ttgcgccgcc gagcgcgtcg agttcgcgta
     3061 ccaaatgccc tttgccgatg ccgccgatgg aagggttgca tgacatttgt ccgagcgttt
     3121 cgatattgtg tgtgagcaaa agcgtctgcg cgcccatgcg ggcggcggcg agcgcggctt
     3181 cggtaccggc atggccgcca ccgacgacaa tcacgtcgta ggttttgggg taaatcatgt
     3241 gggtcatgtg tgtattgcct gacggtgttt cagacgg
//
LOCUS       DAWKOG010000186         3259 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.186, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000186 DAWKOG010000000
VERSION     DAWKOG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.186"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            124..2151
                     /gene="uvrB"
                     /locus_tag="WJK66_001930"
     CDS             124..2151
                     /gene="uvrB"
                     /locus_tag="WJK66_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEZ7579582.1"
                     /translation="MEVIRYPDSPFKLHQPFPPAGDQPTAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLIQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IEFFAREQRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRTVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAIDETERRREKQMKFNEEHGIVPQQIKKQVKDIIDGVYHEEDRSKGRLKGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVLRDRIRNIKESLLFGAE"
     gene            2187..3071
                     /locus_tag="WJK66_001931"
     CDS             2187..3071
                     /locus_tag="WJK66_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579583.1"
                     /translation="MTSIILELQSLAANPNSDVEELVSKTLMVARKLKINELTEWCNN
                     ELQGYSSKNLPKYRIFYGELKAFNPYRGDVPFMLPDELRERVTRVEYTSSISEIRNLL
                     KTKNNNFIRILSSKEKQFLMNLQDTPFPLEPKIIIYPINLMNIQSKVRQLILAWALRL
                     EEEGILGDGIQFTKSKQEKAMSNQFNIQNMQGFIGDIFGGNVQQNIQDGIHIEKENFN
                     KLANFLAKNGIPSAELEDLRNALIEDGQPTDKKFGSKVSAWIGNIITKASTGAIDVSV
                     GTIAGILTNAISKYYGLM"
BASE COUNT          989 a          868 c          681 g          721 t
ORIGIN      
        1 ttgggtagcg attgggtttc agacggcata cattcaggat ttcaccgcct tatgccgtct
       61 gaaagacgtt tccgacccat ccctactaca cgctttgtcc gcaaacgcat cacaggcaag
      121 attatggaag tcatccgata ccccgattcc cctttcaaac tccaccaacc cttcccgccc
      181 gcaggcgacc agcctaccgc tattgccggc ttgctcgaag ggctttcaga cggccttgcc
      241 taccaaaccc tgctcggcgt aaccggttcg ggcaaaacct acaccatggc gaacgtcatc
      301 gcccaaagcg gccgacccgc catcatcatg gcgcacaaca aaacccttgc cgcccagctt
      361 tatgccgaaa tgcgcgagtt tttccctgaa aacgcggtgg aatatttcgt ctcctactac
      421 gactattacc aacccgaagc ctatgtgcca agccgcgatt tgttcatcga aaaagacagt
      481 gcgatcaacg aacacattga acaaatgcgc ctttctgcca ccaaaaacct gatgacgcgc
      541 gatgacgtga ttatcgttgc cactgtgtcc gccatttacg gtatcggcga cccgaccgag
      601 tatcagcaaa tggtgttgtc tgtcaaagaa ggcgacacca tcgagcagcg cgacatcatc
      661 gccacgctcg tttccatgca gtacgaacgc ggcgatttgg acttcaaacg cggcagcttc
      721 cgcgtgcgcg gcgacgtgat tgacgtgtac cccgccgaaa gctccgaaaa cgccttgcgt
      781 atcagcctgt tcgacgacga aatcgaccgc ctcgatatgt tcgacccgct ttcaggcagc
      841 cttattcaac gcgtcggccg ctacaccgtc ttcccgtcca gccactacgt taccccgcgc
      901 gacaccgtat tgcgcgcctg cgagtccatc aaagaagaat tgcgcgaacg catcgaattt
      961 ttcgcccgcg aacaacgccc cgtcgagcaa caacgcatcg aacagcgcac ccgtttcgac
     1021 ctcgaaatgc tctacgaaat gggcttctgc aaaggcattg aaaactactc ccgccacttc
     1081 tccggcaaaa aagaaggcga accgccgccc acgctgatgg actacctgcc cgacaacgcc
     1141 atcatgttca tcgacgaaag ccacgttacc gtaacccaaa tcggcggcat gtacaaaggc
     1201 gacgcatcgc gcaagcaaaa cctcgtcgac tacggcttcc gcctgccttc cgcccgcgac
     1261 aaccgcccgc tcaaattcca cgaatttgaa aaagtcatgc cgcaaactgt cttcgtttcc
     1321 gccactccag ccaaatacga agaagaacac gccggacaag tggtcgaaca agtcgtccgt
     1381 cccaccgggc tggttgatcc ccaaatcatc atccgcaccg tcgccaccca agtcgatgat
     1441 ttaatgagcg aaatcaacga ccgcatccaa aaaggcgaac gcgtgctcgt taccaccctc
     1501 accaaacgca tggcggagca actcaccgac tattacagcg aactcggcat caaagtgcgc
     1561 tacctgcaca gcgacatcga caccgtagag cgcgttgaaa tcattagaga tttacggctc
     1621 ggactgtttg acgtactcgt cggtatcaac ctcttgcgtg aaggcctcga catccccgaa
     1681 gtctccctcg ttgccatcct cgacgccgac aaagaaggct tcctgcgctc ccaccgcagc
     1741 ctgattcaaa ccataggccg cgccgcgcgc aacgtgaacg gcgtcgccat cctgtacgcc
     1801 gacaaaatca ccgactccat gaaagccgcc atcgacgaaa ccgaacgccg ccgcgaaaaa
     1861 cagatgaaat tcaacgaaga acacggcatc gtgccgcagc agattaaaaa acaggtcaaa
     1921 gacatcatcg acggcgtgta ccacgaagaa gacagaagca aaggccgtct gaaaggcaaa
     1981 aacaaggtca aagtcggcga aatccacaac gaagaagacg cgattaaaga aatcgccaaa
     2041 ctggaaaaag ctatgcagca ggcggctagg gatttgcagt ttgaagaagc ggctgttctt
     2101 cgtgatagga tacggaatat taaagagagt ttgttgtttg gggcggaata attttattat
     2161 ttttatttaa catggagatt aattaaatga cttctattat tttagaactc caaagcctag
     2221 ctgccaatcc aaatagtgat gtagaagagt tagtgagtaa aacattaatg gttgcaagaa
     2281 aattaaaaat taatgaatta actgaatggt gtaataatga attacaaggt tattcatcaa
     2341 aaaatttacc aaaatataga atattttatg gagagttaaa agcttttaac ccttatcgtg
     2401 gcgatgttcc atttatgctt cccgatgaat tgagagaacg agtaacaaga gttgaatata
     2461 cctcatcaat tagcgaaata agaaacttac taaaaacaaa aaataataat tttatacgaa
     2521 ttctatctag taaagaaaaa cagtttttaa tgaatttaca agatactcca tttccacttg
     2581 aacctaaaat aattatttat ccaattaact taatgaatat tcaatcaaaa gtaagacaac
     2641 taattttagc ttgggcatta cgactggaag aggaaggcat tttaggagac ggtatccaat
     2701 ttacaaaatc aaaacaggag aaagcaatgt caaaccaatt caatattcag aatatgcaag
     2761 ggtttattgg ggatattttt ggcggtaatg ttcagcaaaa tattcaagac ggtattcata
     2821 ttgaaaaaga aaatttcaat aaactggcta actttttagc aaaaaacggg attccatctg
     2881 ccgaattaga agatttacga aatgcactga tagaagatgg tcagcccaca gataaaaaat
     2941 tcggctcaaa agtaagtgca tggattggca atattattac aaaggcttct accggtgcaa
     3001 ttgatgtttc agtaggtact attgcaggaa tattaactaa tgcgatttca aaatattatg
     3061 gcttaatgta agcaaatgca atatgtgcgg ctccaccaca cacgccgtcc ccaaccgccc
     3121 aaaaccgcgt gcctgcctgc ggcttacccc ctaccaccca accaacacag caagcgtagg
     3181 ttggatactt gtatccgaca aaacctttaa cattcccatc attgcaatcc attgtagcaa
     3241 tgcctaaaat gtcggattc
//
LOCUS       DAWKOG010000187         3191 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.187, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000187 DAWKOG010000000
VERSION     DAWKOG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.187"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            152..2917
                     /locus_tag="WJK66_001932"
     CDS             152..2917
                     /locus_tag="WJK66_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ7579584.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQQYFVQTGGLKFNSNSGKWER
                     DLQRQFWKTSYYKQYKDPQKLKKYIEDQDKDWRENLAPQYDITPIDPSSLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNSYTNLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNTVGYRFGGEYTGYYGSDDEFKRAFGENSPTYREHCNQSCGIYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGDIPSWVSST
                     GLAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSNFRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEDVTCNADGLCNGKYGGTSKSVLTNFARGRTFL
                     MTMSYKF"
     gene            2960..>3191
                     /locus_tag="WJK66_001933"
     CDS             2960..>3191
                     /locus_tag="WJK66_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579585.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVIYESSFPQKRESRHSMLRQFI"
BASE COUNT          907 a          790 c          764 g          730 t
ORIGIN      
        1 acaacgctgt accggttttt gttaatccac tataaatatc caattgaaat cttcagacgg
       61 tatatcgaat ttacactttt taatgtttat gccgcctaaa aaaatgctaa tatatttctt
      121 aattgtctga ctgtttattg ttgaggaaaa tatgagatct tctttccggt tgaagccgat
      181 ttgtttttat cttatgggtg ttatgctata tcatcatagt tatgccgaag atgcagggcg
      241 cgcgggcagc gaggcgcaga tacaggtttt ggaagatgtg cacgtcaagg cgaagcgcgt
      301 accgaaagac aaaaaagtgt ttaccgatgc gcgtgccgta tcgacccgtc aggatatatt
      361 caaatccagc gaaaacctcg acaacatcgt acgcagcata cccggtgcgt ttacacagca
      421 agataaaagc tcgggcattg tgtctttgaa tattcgcggc gacagcgggt tcgggcgggt
      481 caatacgatg gtggacggca tcacgcagac cttttattcg acttctaccg atgcgggcag
      541 ggcaggcggt tcatctcaat tcggtgcatc tgtcgacagc aattttattg ccggactgga
      601 tgtcgtcaaa ggcagcttca gcggctcggc aggcatcaac agccttgccg gttcggcgaa
      661 tctgcggact ttaggcgtgg atgacgtcgt tcagggcaat aatacctacg gcctgctgct
      721 aaaaggtctg accggcacca attcaaccaa aggtaatgcg atggcggcga taggtgcgcg
      781 caaatggctg gaaagcggag catctgtcgg tgtgctttac gggcacagca ggcgcagcgt
      841 ggcgcaaaat taccgcgtgg gcggcggcgg gcagcacatc ggaaattttg gcgcggaata
      901 tctggaacgg cgcaagcagc aatattttgt acaaacaggc gggttgaaat tcaattccaa
      961 cagcggaaaa tgggaacgcg atttgcaaag gcaattctgg aaaaccagtt actataaaca
     1021 atacaaagac ccccagaaac tgaaaaaata catcgaagac caagataaag actggcggga
     1081 aaacctggcg ccgcaatacg acatcacccc catcgatccg tccagcctga agcagcagtc
     1141 ggcaggcaat ctgtttaaat tggaatacga cggcgtattc aataaataca cggcgcaatt
     1201 tcgcgattta aacaccaaaa tcggcagccg caaaatcatc aaccgcaatt atcaattcaa
     1261 ttacggtttg tctttgaact cgtataccaa cctcaatctg accgcagcct acaattcggg
     1321 caggcagaaa tatccgaaag ggtcgaagtt tacaggctgg gggcttttaa aggattttga
     1381 aacctacaac aacgcaaaaa tcctcgacct caacaacacc gccaccttcc ggctgccccg
     1441 cgaaaccgag ttgcaaacca ctttgggctt caattatttc cacaacgaat acggcaaaaa
     1501 ccgctttcct gaagaattgg ggctgttttt cgacggtcct gatcaggaca acgggcttta
     1561 ttcctatttg gggcggttta agggcgataa agggctgctg ccccaaaaat caaccattgt
     1621 ccaaccggcc ggcagccaat atttcaacac gttctacttc gatgccgcgc tcaaaaaaga
     1681 catttaccgc ttaaactaca gcaccaatac cgtcggctac cgtttcggcg gcgaatatac
     1741 gggctattac ggctcggatg acgaatttaa gcgggcattc ggagaaaact cgccgacata
     1801 cagggaacat tgcaaccaga gctgcggaat ttatgaaccc gtattgaaaa aatacggcaa
     1861 aaagcgcgcc aacaaccatt cggtcagcat tagtgcggac ttcggcgatt atttcatgcc
     1921 gttcgccagc tattcgcgca cacaccgtat gcccaacatc caagaaatgt atttttccca
     1981 aatcggcgac tccggcgttc acaccgcctt aaaaccagag cgcgcaaaca cttggcaatt
     2041 tggcttcaat acctataaaa aaggattgtt aaaacaagat gatacattag gattaaaact
     2101 ggtcggctac cgcagccgca tcgacaacta catccacaac gtttacggga aatggtggga
     2161 tttgaacggg gatattccga gctgggtcag cagcaccggg cttgcctaca ccatccaaca
     2221 tcgcaatttc aaagacaaag tgcacaaaca cggttttgag ttggagctga attacgatta
     2281 tgggcgtttt ttcaccaacc tttcttacgc ctatcaaaaa agcacgcaac cgaccaactt
     2341 cagcgatgcg agcgaatcgc ccaacaatgc gtccaaagaa gaccaactca aacaaggtta
     2401 tgggttgagc agggtttccg ccctgccgcg agattacgga cgtttggaag tcggtacgcg
     2461 ctggttgggc aacaaactga ctttgggcgg cgcgatgcgc tatttcggca agagcatccg
     2521 cgcgacggct gaagaacgct atatcgacgg caccaacggg ggaaatacca gcaatttccg
     2581 gcaactgggc aagcgttcca tcaaacaaac cgaaactctt gcccgccagc ctttgatttt
     2641 tgatttttac gccgcttacg agccgaagaa aaaccttatt ttccgcgccg aagtcaaaaa
     2701 tctgttcgac aggcgttata tcgatccgct cgatgcgggc aatgatgcgg caacgcagcg
     2761 ttattacagc tcgttcgacc cgaaagacaa ggacgaagac gtaacgtgta atgctgatgg
     2821 cttgtgcaac ggcaaatacg gcggcacaag caaaagcgta ttgaccaatt ttgcacgcgg
     2881 acgcaccttt ttgatgacga tgagctacaa gttttaaagg cagcccgcgt tttgtggaaa
     2941 accgcaaaaa aaagccaaaa tgccgtctga aagcccttca gacggcattt gtttccccaa
     3001 acgcatcatc ctgccgcaag cctatgccaa tccgttttat cgcatcggca actcaaagaa
     3061 aaatccattt cattcccacg cagggaagcc ggtttttgat ttcggttatt tttggttgtt
     3121 tcgggtaatt tatgagtcgt cattcccgca aaagcgggaa tctagacatt caatgctaag
     3181 gcaatttatc g
//
LOCUS       DAWKOG010000188         3076 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.188, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000188 DAWKOG010000000
VERSION     DAWKOG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.188"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            133..711
                     /locus_tag="WJK66_001934"
     CDS             133..711
                     /locus_tag="WJK66_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ7579586.1"
                     /translation="MFRHNGEILLSGINIKLKSGDSLLIRGPSGCGKTSLLRALAGLW
                     PFGSSGKVSRPPHQDILFLPQRPYTAQGSLRDAVCYPDIDKQHPELIEAMNTCRLGYL
                     IDKLDKTDDWQHKLSLGELQRVAFVRALLSKPKIVLLDEATAALDEPAEAALYRALKQ
                     KLPDSIIISIGHRSTLNAFHNMRLDVGNVACG"
     gene            816..1484
                     /gene="hda"
                     /locus_tag="WJK66_001935"
     CDS             816..1484
                     /gene="hda"
                     /locus_tag="WJK66_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEZ7579587.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            1481..2149
                     /locus_tag="WJK66_001936"
     CDS             1481..2149
                     /locus_tag="WJK66_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ7579588.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCHLFGITNIIGTQLETGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVDEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
BASE COUNT          870 a          861 c          608 g          737 t
ORIGIN      
        1 gtgaaaaagc tggcgtaact tttccggata caaaaaaacg ccgaaatcca cgtcttccga
       61 agatttcagc gtttttttac tctatttgtt ccttgtgcaa aaactaacag tcccccacag
      121 gaaaacgtcg ccctgttccg ccacaacggc gaaatcctgt tgagcggcat caacatcaaa
      181 ctcaaaagcg gcgattccct gctgatacgc ggcccgagcg gttgcggcaa aacttcgttg
      241 ctgcgcgcgc tggctgggct ttggccgttc ggcagcagcg gcaaagtcag ccgtccgccg
      301 catcaagaca tcctcttcct gccgcaacgc ccgtacacgg cacagggcag cctgcgcgac
      361 gcggtttgtt atcccgacat cgacaaacag catccggagc taatcgaagc catgaacacc
      421 tgccgcttgg gttatctgat tgacaaattg gacaaaacag acgactggca gcacaaactc
      481 tccctgggcg aactgcaacg cgtcgccttc gtccgcgccc tgctttccaa gcccaaaatc
      541 gtcctgctcg acgaagccac cgccgccttg gacgaaccgg ccgaagccgc gctctaccgc
      601 gccttaaagc aaaaactgcc cgacagcatc atcatcagca tcggacaccg aagcacgctc
      661 aacgcgttcc acaatatgcg cctcgatgtc ggcaacgttg cctgcggttg aacgatgccg
      721 tctgaagccg ttttcagacg gcatcgttca aattgataaa gcacgcgaaa acaacgtaca
      781 ataaacccgt ttaccacccc tcggaatgcc gaaccgtgaa ccagcttatt ttcgactttg
      841 ccgcacacga ctatccgagt ttcgacaaat tcctcggcac ggaaaacgcg gaactggtct
      901 atgtcctccg acacaaacac ggacagttta tctatgtctg gggtgaagaa ggtgcgggca
      961 aaagccatct tttgcaggcg tgggtcgcac aggcgctcga agccggcaaa aacgccgcct
     1021 atatcgatgc cgcctccatg ccgctgaccg atgccgcgtt cgaggcggaa tacctcgccg
     1081 tcgatcaagt cgaaaaactg ggcaacgaag aacaagccct gcttttttcc atcttcaacc
     1141 gcttccgcaa cagcggcaaa ggctttttgc tgctcggttc ggaatacacg ccccagcagc
     1201 tcgtcatccg cgaagatttg cggacgcgta tggcgtactg cctcgtttac gaagtcaagc
     1261 ccttaaccga ccaagaaaaa atcgacgcgc tcgccagtat ggcggcggca cgccaagtaa
     1321 ccgtcgattc cgaaattttc gaatacctgc tcaaacactg gcggcgcgat atggacagcc
     1381 tgatgatgat gctcgacacg ctggacaact acgccgtcac aatgggcaaa cgcatcaccc
     1441 tgccgctttt gcgccagctt ttgaaacaac aggaaaccca atgaaaaacc tcgccatctt
     1501 cgacctcgac aacaccctca tcaacaccga ttccgaccac gcctggccgc aatacctcat
     1561 caaaaaaggc ttggttgacg ctgccgaaac cgaagcgcaa aacgaaaaat tctaccgcga
     1621 ctaccaaaac ggttgcctcg acatcgacgc attcctcaaa ttccaccttg ccccgctcgc
     1681 tcgttacagc aaagaagagc tggcagagtt tcaccgcgaa tttatggcgg aatacatcat
     1741 cccccacatc tcgcctatgc agcgtatgct ggtgcagagc caccaaatgg caggcgacga
     1801 aacccttgtg atttcctcaa ccaacgagtt catcatcacc cccgtctgcc accttttcgg
     1861 catcaccaac atcatcggca cacaactcga aaccggttct gacggccgct acaccggcaa
     1921 ttacatcggc acgcccagcc tcaaagaagg caaaatcacc cgcctgaacc aatggctcgc
     1981 cgaacgcggc gaaacgctcg aaagctacgg caaaacctat ttttacagcg actccaaaaa
     2041 cgacctgccg ctgctgcgcc tcgtcgacga acccgtcgcc gtcaaccccg atgccgagct
     2101 ggaaaaagaa gccaaagaaa aaggctggcc ggttttgaat ttcaaatgac gcaatgccgt
     2161 ctgaagcccg attcggcgtt cagacggcat tgaggctgaa atccaaaaaa ctcaaagcca
     2221 caggaattta tccggaaaaa caaaaaacac aggaattcat cgggaaaaac aacaatcttt
     2281 ccaccgtcat tcccacgaaa gtgggaatct tgatccgtcc gcacggaaac ttatatttcg
     2341 tcatttgcac ccgacataat tcaatacttt attttgcaat aaattaacgg atgctgacaa
     2401 attagattag attaattacc ctattatcta tattgggtta tcgggttgct aattgaacag
     2461 agcgtagcaa accataaagt gagtgtttcc acccactctg catttactat gaagtggtta
     2521 ttaaatatga taatgagacc tattaaattt agtatggtaa atacgttatt atttattgtt
     2581 atatgtagtt cattttttga tctgctcgtt caattatgta caattttatt tcatagccaa
     2641 aaaatatact ttattacatt atttttatta tttattttta attttgttac aaaatctatc
     2701 tatatggcaa ttatttatcc tattttatat ttttttacga taaaaaaata ttacccttac
     2761 tctaggaaag taataattct attatcatta gcattatcta tatattttag ttttatggac
     2821 ttttactttt tttccatata ttcagataac cttagctatg aaacggagcc tttacattta
     2881 tacatcccta ttattattaa ttttttctca cttttagttt ctaattttat tttatctttt
     2941 atcaacaagt aaatgtatat acaaaataca atccaaaaga tcctaagcaa accaccaatc
     3001 ccacaccacc cttaccggtt caaatccgca caaaaaccca catcccgtca ttcccacgaa
     3061 agtgggaatc taggac
//
LOCUS       DAWKOG010000189         3035 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.189, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000189 DAWKOG010000000
VERSION     DAWKOG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.189"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(292..681)
                     /locus_tag="WJK66_001937"
     CDS             complement(292..681)
                     /locus_tag="WJK66_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEZ7579589.1"
                     /translation="MNPHEKLLTTDNPILRQRAKAMRQEMSGAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHTEQAAYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLTEILLVLQELEKQAAR"
     gene            complement(1253..1378)
                     /locus_tag="WJK66_001938"
                     /pseudo
     CDS             complement(1253..1378)
                     /locus_tag="WJK66_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1424..2557)
                     /gene="carA"
                     /locus_tag="WJK66_001939"
     CDS             complement(1424..2557)
                     /gene="carA"
                     /locus_tag="WJK66_001939"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEZ7579590.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYL
                     VRNKTVAIADIDTRRLTTLLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWELGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAQDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT          632 a          777 c          847 g          779 t
ORIGIN      
        1 gctgcaacct accccctccc ccgtggggga gggctgggga gagggcattc tccgaatttc
       61 ggcaaccttt cctaacactc taaccgccaa gacacaagcc ttgcggcttg ttgccctctc
      121 tctagctctc tcccacggga gagaggatgg ggtggctgtt ggggctaagg tttctgtaaa
      181 ctaatcaggt tatgcaggct gctttttgtt tttcagacgg cctttataaa tgctcaattc
      241 agtcggcttt atcctattat tttcaataat ccaatcaaaa tcagccatct gctatcgtgc
      301 agcttgcttt tccaattctt gcaacacgag caggatttct gtcagcacat cgtttgtctg
      361 ctgtaaaatc tcgtgattcc aaaaccgcag tacggtaaag cccaagctgt tgagatatgc
      421 cgtccgcgcg tggtcgtatg cggcttgttc tgtgtgctgc cctccgtctg cttcgacaat
      481 caacttgggc gttacgcaca taaaatcaac aatatagttt cccatcggct gctggcggcg
      541 gaatttatag ccgttcagac gacctgcccg caggtgctgc cacaattttg cttccgcccc
      601 gctcatttct tggcgcatgg ctttggcgcg ttggcgcaag atggggttgt cggtggtcaa
      661 gagtttctcg tgcgggttca ttttgttgtc gtgctgtttg ctgccctctc tctaactctc
      721 tcccatggga gagaggacgt ggtggcggtt gggattaagg gttttgcaag gaaaacaggt
      781 tgtgcaggct gctttttatt tttcagatga cctgcaatca acggttgtaa atccaagttg
      841 cttttcaaac cgcctgtaac cgcaaaactt gctgcaacct accccctccc ccgtggggga
      901 gggctgggga gagggcattc tccgaatcgc ggcaatcttt ccctaattcc ctaactgcct
      961 caatacaagc ctttcggctt gttgccctct ctctaactct ctcccacggg agagaggacg
     1021 gtgcggctgt agtgattaag ggttttgcaa ggaaaacaag gaaaacaagt tgtacaggct
     1081 gttttttatt tttcaagcga cctgaaatca acgcttgtaa atccaagtcg cttttcaaac
     1141 tgcctgcaac cgcaaaatct gctgcaacct accccctccc ccgtggggga gggctgggga
     1201 gagggcattt tccaagttgc ggcaatcttt cccaaatccc ttagtcttct aaaatacaag
     1261 ccttacggtt gttgccaaat gaaacttctt ccatacccaa aacttgcgtg taaaacgcaa
     1321 tggttcggtc aatgtcggca acggtcagta tcagatggtc gagtgcgcta atcttcattt
     1381 cccgctttct ttttcaggcg gcggcagtgc cgtctgaaag ccattatgcc cgttttgccg
     1441 ctttcatatt gccaatgaat ttgtcaaaca aatagccgac atcttgcgga ccggggctgg
     1501 cttcggggtg gccttggaag cagaacacag gtttgtcggt cagctcgatg ccttgcaaag
     1561 tgttgtcaaa caaggatttg tgggtaatgc gtgcgttagc gggcagggtg tcggcatcaa
     1621 cggcaaaacc gtggttttgg ctggtgatga cgactttgcc gctgtccaaa tcttgcacag
     1681 ggtggttcgc accgtggtgg ctgaagcgca ttttcagggt tttcgcgccg atagcgaggc
     1741 tgatgagctg gtgtcccaag cagatgccga aaatcggttt gccgctttcc atcagttttt
     1801 gcacggcttc gatggcgtag gtgcaaggct cggggtcgcc ggggccgttg gacaggaaca
     1861 cgccgtcggg attgagtgcc aacacgtctt gtgcgctcgt ttgggcgggg acgacggtca
     1921 ggcggcagcc gcgcgaggcg agcatacgca ggatgttggt tttcacgccg aaatcgtagg
     1981 cgacgacgtg gtaaggctgt tcgtcagggg taacgaaacc cttgcccaat tcccattcgc
     2041 cttccgtcca ttcgtaagtt tccgtgcagg aaacttcttt tgccaagtct ttgccgacca
     2101 tactgccgaa cgcggcgatg agttcttgcg ctttttcgat tgtggcatcc gcaccggtca
     2161 gaatcgcgcc gccttgcgcg cctttttcgc gcaacagcgt ggtcaggcgg cgggtatcga
     2221 tgtcggcgat ggcgacggtt ttgttgcgta ccaaatagtc gtgcaggctt tcggaggcgc
     2281 ggaagttgct gtgcaagagc ggcaggtcgc ggataatcag gccggcggca taaacgctgc
     2341 ggctttcttc atcttcggcg ttggtgccgg tgttgccgat gtgggggtag gtgagggtaa
     2401 cgatttgttt gcagtaggac gggtcggtca ggatttcctg atagccggtc atcgaagtat
     2461 tgaacacgac ttcgccggaa gtcgaacctt cgtaaccgat tgatgtgccg tggaatacgc
     2521 tgccgtcagc gaggacgagg agggcggggg tgctcatgat gggaatcctg tttttaataa
     2581 aattcttgac aatgccgtct gaagggactt cggcaggggg catccggtcg gaaaaatccg
     2641 gtcaaaaaaa aacacgccgc gcaagataga cgcgtaacgt gcttttcggg caagtgccta
     2701 taccctgaaa gcagcatatt ttaagacgaa acatacccga tttcaagcgc aaacgcggca
     2761 ggcgggctga tatgttcgcg cgaaaacgga aatccgtggc gtaaaaccgc ccgctccgcg
     2821 cccactatgc ccgttacgca tttccccacg tccgtccggc gaaactatgg gaatacccga
     2881 atcgtcagga atacccgaac cgtcattccc gcgcaggcgg gaatccagac ctgtcggcac
     2941 agaaacttat cgggtacaac ggtttcttga gattttacgt cctagattcc cgcctgcgcg
     3001 ggaatgacgg tatcggtttg tcggcgtttc atttg
//
LOCUS       DAWKOG010000190         3027 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.190, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000190 DAWKOG010000000
VERSION     DAWKOG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.190"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            44..2302
                     /locus_tag="WJK66_001940"
     CDS             44..2302
                     /locus_tag="WJK66_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEZ7579591.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINAFNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMRHGVLRRSFTVFGVRFNVCKFLSVAQKELAVIRWEAVSVDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDSLSYIAAQTVANPFGVEQFIVNAEQ
                     TFAGSFKALGGSQTDWQVSNSFEVEVGGTPKTFEKRVIVTTSRDYQGLEAVKAAGRAL
                     SEKVAGVAFETLLDAHKAGWLHRWEIADVVIEGSDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDEGYLAKEGLE
                     VLVEVSRFWADRVHFSKRNGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVRAAELEKWADISANMYRPHDEELGVFVQHDGFLDKDIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNIDEKRRNFDFYEPMTVHESSLSPC
                     IHSILAAELGKEEKAVEMYRRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGKLSFAPFLPGAWTGYAFHINYRGRLIKVAVGKENVVFSLLKGGPLDLQVYGKDI
                     TLNGSHTAALEK"
     gene            2315..2980
                     /gene="pgmB"
                     /locus_tag="WJK66_001941"
     CDS             2315..2980
                     /gene="pgmB"
                     /locus_tag="WJK66_001941"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEZ7579592.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGIGIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVGEAEFAELTRRKNDNYVEMIQAVKPEDVYPGILPLLET
                     LKANGKKIALASASKNGPFLLERMGLTHFFDTVADPAAVAHSKPAPDIFLAAAEGVGA
                     DIRHCIGIEDAAAGVAAIKAAGALPIGVGKAEDLGSDIALVSETAGLTYAYLQNVWAQ
                     SGR"
BASE COUNT          764 a          930 c          785 g          548 t
ORIGIN      
        1 atttcaccat tccctcaaat caatccaaac aggagcttca taaatgtaca caagaatcat
       61 ggaaatcagc ccttggacgc tgcgttcggc aaaactggaa aaagaacaca aacggctgca
      121 agagagcctg accagcttgg gcaacggcta tatgggtatg cgcggcagct ttgaggaaac
      181 ctattccgcc gacagccact taggcaccta catcgccggc gtgtggttcc ccgacaaaac
      241 ccgcgtcggc tggtggaaaa acggctatcc caaatatttc ggcaaagcca tcaacgcgtt
      301 caatttcagc aaagtcaaaa tctttgtcga cgggcaggaa gtggacttgg cgaaaaacga
      361 cgttgccggc ttctccgtcg aactcgatat gcggcacggc gtgttgcgcc gctcgtttac
      421 cgtattcggt gtgcgtttca atgtgtgcaa attcctgtcc gtcgcgcaaa aagagctggc
      481 ggtcatccgc tgggaagccg tatccgttga cggtaaaacc caccaagtcc gcatcgattc
      541 catcatcgat gccgacgtga aaaacgaaga ctccaactac gaagaaaaat tctggcaggt
      601 attggacaaa ggcgtttcag acagtctctc ctacattgcc gcccaaaccg ttgccaaccc
      661 tttcggcgtg gaacaattta tcgtcaacgc cgagcaaact ttcgccggca gcttcaaagc
      721 cctcggcggc agccaaaccg actggcaagt ctccaattct ttcgaggtcg aagtcggcgg
      781 cacgcccaaa acctttgaaa aacgggttat tgttaccacc agccgcgatt atcagggctt
      841 ggaagcagtg aaagccgcag gccgcgcctt gtcggaaaaa gtcgcaggcg ttgcgtttga
      901 aaccttgctg gacgcgcaca aagcaggctg gctgcaccgt tgggaaatcg ccgacgtggt
      961 catcgaaggc agcgacgaag cgcagcaagg catccgtttc aatctgttcc aactgttctc
     1021 cacctactac ggcgaagatg cgcgcctgaa catcggcccc aaaggcttta ccggcgaaaa
     1081 atacggcggc gcgacctatt gggataccga agcctatgcc gtaccgctct acctcgcact
     1141 ggcggaaccc gaagttaccc gcaacctgct gcaataccgc cgcaaccaac tgccgcaggc
     1201 gcagcacaac gcgcgcgaac agggcttggc gggcgcactc tatccgatgg taacgtttac
     1261 gggtatcgag tgccacaacg aatgggaaat caccttcgag gaaatccacc gcaacggcgc
     1321 gattccttac gccatctaca actacaccaa ctacaccggc gacgagggct atcttgccaa
     1381 agaaggcttg gaagtcttgg tcgaagtgtc ccgcttctgg gcggaccgcg tccacttctc
     1441 caaacgcaac ggcaaataca tgattcacgg cgtaaccggc ccgaacgaat acgaaaacaa
     1501 catcaacaac aactggtaca ccaacaccct cgccgcctgg gtattggact acacccgcga
     1561 agccctggcg aaatacccgc gcccggattt gaacgtgcgt gccgccgagt tggaaaaatg
     1621 ggcggacatc agcgcgaata tgtaccgtcc gcatgacgaa gaactcggcg tattcgtcca
     1681 acacgacggc ttcctcgaca aagacatccg tcccgtgtcc gcgctttcgc ccgacgattt
     1741 gccgctcaac cagaaatggt cgtgggacaa aatcctgcgt tcgcccttca tcaagcaggc
     1801 ggacgtgttg caaggcatct acttcttcgg cgaccgtttc aatatcgacg aaaaacgccg
     1861 caacttcgat ttctacgaac cgatgaccgt gcatgaaagc tcgctgtcgc cctgtatcca
     1921 ctctattctc gccgccgaac tgggcaaaga agaaaaagcc gtggaaatgt accggcgcac
     1981 cgcccgcctg gacttggaca actacaacaa cgacacagaa gacggcctgc atatcacctc
     2041 catgaccggt tcgtggctcg ccatcgtcca aggtttcgcc caaatgaaaa cttggggcgg
     2101 caaactcagc ttcgcaccgt tcctgccggg cgcgtggaca ggctatgcct tccacatcaa
     2161 ctaccgaggc cgtctgatta aagtcgccgt cggcaaagaa aacgttgtct tcagcctgct
     2221 caaaggcggg ccgctcgatt tgcaggtgta cggcaaagac atcacgctca acggcagcca
     2281 caccgccgcg ttggaaaaat aaggagggcg caaaatgacg tttaccgcag tcctcttcga
     2341 tctcgacggc gtgattaccg ataccgccga ataccactac cgcgcgtgga aaaagctcgc
     2401 cgaagaactg ggcatcggca tcgaccgcaa gtttaacgaa cagctcaaag gcgtgtcgcg
     2461 cgacgattcg ctcaaacgca tcctcgcgca cggcggcaaa accgtcggcg aagccgagtt
     2521 cgccgaactg acccgccgaa aaaacgacaa ctacgtcgag atgattcagg cagtcaaacc
     2581 cgaagacgtg taccccggca ttttgccgct gctggagaca ttaaaagcaa acggcaaaaa
     2641 aatcgccctc gcgtccgcca gtaaaaacgg cccgttcctg ctcgaacgca tggggctgac
     2701 ccactttttc gacactgtcg ccgaccctgc cgccgtcgcg cattccaaac ccgcccccga
     2761 catcttcctc gccgccgccg agggcgtggg cgcggacatc cgccactgca tcggcattga
     2821 agacgccgcc gccggcgttg ccgccatcaa agccgccggc gcactgccca tcggcgtagg
     2881 caaagccgaa gacttgggca gcgacatcgc gctggtgtcc gagaccgccg ggctgaccta
     2941 cgcctacctg caaaacgtgt gggcgcagtc gggcaggtaa agcgcgtcag ataaagtgtt
     3001 aaggaagtaa aaggccgtct gaacagt
//
LOCUS       DAWKOG010000191         3007 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.191, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000191 DAWKOG010000000
VERSION     DAWKOG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.191"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            335..658
                     /locus_tag="WJK66_001942"
     CDS             335..658
                     /locus_tag="WJK66_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ7579593.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSLVLIELSD
                     PSEAV"
     gene            772..2949
                     /locus_tag="WJK66_001943"
     CDS             772..2949
                     /locus_tag="WJK66_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ7579594.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKTLDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRPTRKPLFEVRAEAGNRKHFGLDADVSGSLNTEGTLRGRLVS
                     TFGRGDSWRRRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTAATDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPSSFAQTIPQYGTRRQIGGYLATRFRPADNLSL
                     ILGGRYSRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLEGRLNASAAVYRARKNNLATAAGRDLSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQSETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT          734 a         1056 c          754 g          463 t
ORIGIN      
        1 tcagaagctc cgttttagct tcgcagaaac tccgcttcct tcgaaagctc cgttttcaga
       61 cgaccttttg gaacaccaca ggcacacgca tcgaacggct gaatcaaaga ttcagaccga
      121 tggcagtccg cacccgagtt tatgcggcaa acagcgaggc tacggcaacc cgtcccctct
      181 ccctgtggga gagggttagg gagagggcgg taagccgcag gcttacatca aagccgataa
      241 cgcttccgtt acaactccgc ccactgaaag cagcctgcaa cgaagccaaa acgacaaacc
      301 gcatcgtaaa ccacccaacc cataggagaa ccccatgcaa aacgaaacca tcaacctgaa
      361 acagcacctt gccgccatca aagaatactg gcagcccgaa atcatcaacc gccacgggtt
      421 ccaattccgc ttggtcaaac ttttgggcga ttacggctgg catacgcacg gatacagcga
      481 caaagtgctg tttgccgtgg agggcgacat ggcggtggac ttcgccgacg gcggcagcat
      541 gacgatacgc gagggcgaga tggcggtcgt gccgaagtcg gtgtcgcacc gcccgcgttc
      601 ggaaaacggc tgctcgttgg tgctgattga gttgtccgac ccgtccgagg ccgtctgaaa
      661 acgaagtttc cgaaggaagc tgagtttctg cgaagctaaa agcagcctgc accttcaatc
      721 aatatgccga aaatacaacc ccaccgcaca ccaacacaca aaggaaatcc catgacacgc
      781 ttcaaatatt ccctgctgtt tgccgccctg ttgcccgtgt acgcgcaggc cgatgtttct
      841 gtttcagacg accccaaacc gcaggaaagc actgaattgc cgaccatcac cgttaccgcc
      901 gaccgcaccg cgagttccaa cgacggctac actgtttccg gcacgcacac cccgctcggg
      961 ctgcccatga ccctgcgcga aatcccgcag agcgtcagcg tcatcacatc gcaacaaatg
     1021 cgcgaccaaa acatcaaaac gctcgaccgc gccctgttgc aggcgaccgg caccagccgc
     1081 cagatttacg gctccgaccg cgcgggctac aactacctgt tcgcgcgcgg cagccgcatc
     1141 gccaactacc aaatcaacgg catccccgtt gccgacgcgc tggccgatac gggcaatgcc
     1201 aacaccgccg cctatgagcg cgtagaagtc gtgcgcggcg tggcggggct gctggacggc
     1261 acgggcgagc cttccgccac cgtcaatctg gtgcgcaaac gcccgacccg caagccattg
     1321 tttgaagtcc gcgccgaagc gggcaaccgc aaacatttcg ggctggacgc ggacgtatca
     1381 ggcagcctga acaccgaagg cacgctgcgc ggccgcctgg tttccacctt cggacgcggc
     1441 gactcgtggc ggcggcgcga acgcagccgc gatgccgaac tctacggcat tttggaatac
     1501 gacatcgcac cgcaaacccg cgtccacgca ggcatggact accagcaggc gaaagaaacc
     1561 gccgacgcgc cgctcagcta cgccgtgtac gacagccaag gttatgccac cgccttcggc
     1621 ccgaaagaca accccgccac aaattgggcg aacagccgcc accgtgcgct caacctgttt
     1681 gccggcatcg aacaccgctt caaccaagac tggaaactca aagccgaata cgactacacc
     1741 cgcagccgct tccgccagcc ctacggcgta gcaggcgtgc tttccatcga ccacaacacc
     1801 gccgccaccg acctgattcc cggttattgg cacgccgacc cgcgcaccca cagcgccagc
     1861 gtgtcattga tcggcaaata ccgcctgttc ggccgcgaac acgatttaat cgcgggtatc
     1921 aacggttaca aatacgccag caacaaatac ggcgaacgca gcatcatccc caacgccatt
     1981 cccaacgcct acgaattttc ccgcacgggt gcctatccgc agccatcatc gtttgcccaa
     2041 accatcccgc aatacggcac caggcggcaa atcggcggct atctcgccac ccgtttccgc
     2101 cccgccgaca acctttcgct gatactcggc ggcagataca gccgttaccg caccggcagc
     2161 tacgacagcc gcacacaagg catgacctat gtgtccgcca accgtttcac cccctacaca
     2221 ggcatcgtgt tcgacctgac cggcaacctg tcgctttacg gctcgtacag cagcctgttc
     2281 gtcccgcaat cgcaaaaaga cgaacacggc agctacctga aacccgtaac cggcaacaat
     2341 ctggaagccg gcatcaaagg cgaatggctt gaaggccgtc tgaacgcatc cgccgccgtg
     2401 taccgcgccc gtaaaaacaa cctcgccacc gcagcaggac gcgacctgag cggcaacacc
     2461 tactaccgcg ccgccaacca agccaaaacc cacggctggg aaatcgaagt cggcggccgc
     2521 atcactcccg aatggcagat acaggcaggt tacagccaaa gcaaaacccg cgaccaagac
     2581 ggcagccgcc tgaaccccga cagcgtaccc gaacgcagct tcaaactctt cactgcctac
     2641 cactttgccc ccgaagcccc cagcggctgg accatcggcg caggcgtgcg ctggcagagc
     2701 gaaacccaca ccgaccctgc cacgctccgc atccccaacc ccgccgccaa agcccgcgcc
     2761 gccgacaaca gccgccaaaa agcctacgcc gtcgccgaca tcatggcgcg ttaccgcttc
     2821 aatccgcgcg ccgaactgtc gctgaacgtg gacaacctgt tcaacaaaca ctaccgcacc
     2881 cagcctgacc gccacagtta cggcgcactg cggacagtga acgcggcgtt tacctatcgg
     2941 tttaaataag gtcgtctgaa aacggagttt ctgcgaagct atagtggatt aacaaaaacc
     3001 agtacgg
//
LOCUS       DAWKOG010000192         2995 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.192, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000192 DAWKOG010000000
VERSION     DAWKOG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.192"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(230..1219)
                     /gene="pdxA"
                     /locus_tag="WJK66_001944"
     CDS             complement(230..1219)
                     /gene="pdxA"
                     /locus_tag="WJK66_001944"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEZ7579595.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKHLLRA
                     RAEALGKSVVLRDFDPESGGAAYGELEVLHIPAVEAVEAGKLNPANAAYVLQLLDTAL
                     AGISDGIFDGIVTAPLNKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLRV
                     ALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGEGGHL
                     GHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKYH
                     SFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            1379..2806
                     /locus_tag="WJK66_001945"
     CDS             1379..2806
                     /locus_tag="WJK66_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ7579596.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
BASE COUNT          610 a          819 c          848 g          718 t
ORIGIN      
        1 aaaccgaaac cgaacaagcc ggattcccgc aacattccgt cattcccgtg aaaacgggaa
       61 tctagaacct caaactttcg gataatcttt gaatattgcc gtcgcccgaa ggtctggatt
      121 cccgcctgcg cgggaatgac gatttggaaa ttacccgaaa cccaaaaaca actgaaaccg
      181 aacagaccgg attcccgcct gcgcggaaat gacgggtctt ttatcatctt taaaggctgc
      241 cgcgcgccat ctcgacggcg gtctccacgg cagttatcag gctgccggaa tccgccctgc
      301 cggttgccgc caaatcaagc gcggtgccgt gatcgacgga ggtgcggata aagggcaggc
      361 cgagcgtgat gttcacgccc tgtccgaagc tgtggtattt caacacgggc agcccttggt
      421 cgtggtacat cgccaatacg gcatccgcac cttcgagcat aaacggttgg aacaaggtat
      481 ccgccggata cgggccggcg aggtttatcc cttcgcggcg caggttttcg agcgcgggga
      541 taatggtgtc ggtttcttcg tgtccgaggt gtccgccctc gccggcgtgg gggttgagtc
      601 cggcgactag gattttggga tttttgatgc cgaatttgtg ttttaagtcg tgatgcaaaa
      661 tgcgtgcgac gctttcaatc agcggttgcg tgatggcggc ggcaacgtct ttcagcggca
      721 ggtgggtcgt tacgagggcg acgcgcaggc ctttgccggc aagcatcatc acaacctgcc
      781 ccgtgccgct tttttccgcc agatattcgg tgtgtccgct gaaaaaacct gtgcttgcgc
      841 gcgcgtcgtt gatgatgcct ttgttcagcg gcgcggtaac gatgccgtcg aaaatgccgt
      901 ctgaaatgcc tgcgagcgcg gtgtccaaaa gttgcagcac ataggcggcg ttggcgggat
      961 tgagtttgcc cgcctcaacc gcttcgacgg cagggatgtg cagcacttcc agctcgccgt
     1021 atgccgcgcc gcctgattcc ggatcgaagt cgcgcaagac gacgcttttg cccaaggctt
     1081 cggcgcgcgc gcgcaacagg tgtttgtcgc ccaataccgc gcagcggcag ggcaggcgtg
     1141 caaacgccaa gtccaaacaa atatcggggc cgatgccggc aggctcgccg gaagtaacgg
     1201 caaaaacagg ctgtttcatc gtgtttgctc caaacaaaat gcgattctaa cgccgcagcc
     1261 gcgcggcgat gtaaattttt ctgattttgt tgacaatctg ctagaatggg cgtttacaaa
     1321 atttaaaccc tgcttgcata ccgccaatat gtgcgggttt caactttaag gaagcgatat
     1381 gaacgagaac tttaccgaat ggctgcacgg ctgggtcggc gccatcaacg atccgatgtg
     1441 gtcatacttg gtttatatgc ttttgggtac ggggcttttc ttcaccgtaa ccacgggctt
     1501 tgtccaattc cgcctgttcg ggcgcagcat caaagaaatg ctcggcggcc gcaaacaggg
     1561 ggacgaccct cacggcatca cgccgtttca ggcatttgta accggccttg ccagccgcgt
     1621 gggtgtgggc aacatcgcgg gcgtggcaat cgccatcaaa gtcggcgggc cgggcgcggt
     1681 gttttggatg tgggtaaccg ccttaatcgg tatgagttcg gcgtttgtcg aatcttcgct
     1741 ggcgcagctc tttaaagtcc gcgactacga caaccaccat ttccggggcg gccctgccta
     1801 ctacatcacg caagggctgg ggcagaaatg gctgggcgtg ttgttcgccc tgagcctgat
     1861 tttctgtttc ggctttgtgt ttgaagcggt tcagaccaat accatcgccg ataccgtcaa
     1921 agcggcatgg ggttgggagc ctcattatgt cggcgtcgcc ctggtgattt taaccgcgcc
     1981 gattatcttc ggcggcatca ggcgcatatc taaagcggcg gaaatcgtcg tccccctgat
     2041 ggcggttttg tacctcttta tcgcgctttt catcattttg accaatattc cgatgattcc
     2101 ggacgtgttc ggtcagattt tttcgggcgc gttcaaattc gacgcggcag caggcggctt
     2161 actcggcggt ctgatttcgc aaacgatgat gatgggcatc aaacgcggcc tgtattccaa
     2221 cgaggcgggt atgggttccg cgccgaacgc cgccgccgcc gccgaagtga aacaccctgt
     2281 ttcgcaaggt atgattcaaa tgctgggcgt gtttgtcgat accatcatcg tttgttcttg
     2341 caccgccttc atcatcttga tttaccaaca gccttacggc gatttgagcg gtgcggcgtt
     2401 gacgcaggcg gcgattgtca gccaagtggg gcaatggggc gcgggcttcc tcgccgtcat
     2461 cctgtttatg tttgcctttt ccaccgttat cggcaactat gcctatgccg agtccaacgt
     2521 ccaattcatc aaaagccatt ggctgattac cgccgttttc cgtatgctgg ttttggcgtg
     2581 ggtctatttc ggcgcggttg ccaatgtgcc tttggtctgg gatatggcgg atatggcgat
     2641 gggcattatg gcgtggatca accttgtcgc catcctgctg ctctcgccct tggcgtttat
     2701 gctgctgcgc gattacaccg ccaagctgaa aatgggcaaa gaccccgagt tcaaactttc
     2761 cgaacatccg ggcctgaaac gccgtatcaa atccgacgtt tggtaaatcc cgcccttacc
     2821 agagccgctt ccccgcgaag cggcttttcc ctttccgcac actgtaaaaa cagggcgaac
     2881 aagcgtacaa tcccaaccct ttacttttga atccatttcg tttttcagac ggcatattga
     2941 atatagtggt ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt acaag
//
LOCUS       DAWKOG010000193         2995 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.193, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000193 DAWKOG010000000
VERSION     DAWKOG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.193"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            82..243
                     /locus_tag="WJK66_001946"
     CDS             82..243
                     /locus_tag="WJK66_001946"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579597.1"
                     /translation="MRWLNLKNSTSVHNTQGIRTKEAVEQGLRGNNRPEKGIGNRWNY
                     DGFIKELAK"
     gene            293..577
                     /locus_tag="WJK66_001947"
     CDS             293..577
                     /locus_tag="WJK66_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579598.1"
                     /translation="MKVITITLFTSCILSGCYGLEYGMSGGIPHDDWARGSHVSQAKA
                     DAAWKECSDKYMRNGGSGLVEQCMNDKGLIRLSEIRRRQKRAETQRAVIK"
     gene            complement(702..1418)
                     /gene="trmB"
                     /locus_tag="WJK66_001948"
     CDS             complement(702..1418)
                     /gene="trmB"
                     /locus_tag="WJK66_001948"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEZ7579599.1"
                     /translation="MTDTAENQTQNNWQAEHPRSIRSFVLRQSHMTAAQQRAIDTLWG
                     SFGIDYQATPADLDANFGSNRPKILEIGFGMGTATAEIALRLPETDFLAIDVHGPGVG
                     NLLKLIEENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(1540..2943)
                     /locus_tag="WJK66_001949"
     CDS             complement(1540..2943)
                     /locus_tag="WJK66_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ7579600.1"
                     /translation="MNNPDLPYRQALERLSQKQYNFTEVRRLLTEAASAGHPAAAFEL
                     AKHLLDTNSPYQDYKQGIEMLRIAAEQGHPYARYNLAYIQELEGARPETLIPLYRPLA
                     EEGLPEAQVRLMYLLYASRHFEEALKWAKTSAKNNNLHGQYLLAQYCRYGTPPDFETA
                     HLLYRKAAAQGLPEAHWQLGLQYRFGQGTKADTAQAVNHLRAAAQQGYIPAYTPLAEL
                     ILPTAPDEAVHWFQQAAQENDPDAHAALADIYLQGKHPERNYKLALHHAEAAAAERHP
                     EGLRILGDICRYSLGIAPDTEKARHYYRQAAEAGSLAAYQKLISDSALNHPDQYGGIK
                     DSAIRRQRAERLYQKAQALHYGLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMYYFG
                     QGMTADYNEARKWFEKAAAQKDSMALYNLACIHYNGHGVGLDKEKACRYLQEAINNGY
                     GQKSVLQELLQQWQNAV"
BASE COUNT          629 a          623 c          898 g          845 t
ORIGIN      
        1 atacgattaa agtaatgtcg gattcaagaa tccgacctac ggaaaactaa agattttata
       61 cgggctacgg cttgctggct tatgcgttgg cttaatttga aaaacagcac atcggttcat
      121 aatacacaag gtattagaac aaaagaagca gttgaacagg gattgcgtgg taataataga
      181 cctgaaaaag gaataggtaa tagatggaat tatgatggat ttataaagga attagcgaag
      241 taacaggtat tccaaatatt tctgataaat ataaagaaca aggagtaaaa aaatgaaagt
      301 aatcactatt acattgttta ctagctgcat actatctgga tgctatgggc ttgaatatgg
      361 aatgagtggc ggcattcctc atgatgactg ggcaagaggt agccatgtta gtcaagccaa
      421 agcagatgct gcgtggaaag aatgttctga caaatatatg agaaatggtg gttcaggatt
      481 agtagaacaa tgtatgaatg ataaaggatt aatacgttta tctgaaatac gtcgtaggca
      541 aaagagagca gaaacacaaa gagcagtaat taagtaacaa taatgaaata ctactgttcc
      601 cgaacaaagc ggcatttacg caggtgatga cggctatatt ttgaaaaatg ccgtctgtaa
      661 gcaaacgtgc ttcagacggc atttttttat ccggcggtct gttatccgat ccgtttgaat
      721 accaagtccc aaacgccgtg cccgaggcgt ttgccgcgcg cttcgaattt ggtttcgggg
      781 cggtagtccg gggtgggggc gtagtctgcc gccgtatttt gcaggctgtc gaagctactg
      841 aggacttcaa gcatctgctg tgcatattct tcccagtctg tcgccaggtg gatatagccg
      901 ccggttttga gtttgggcag cagtttggcg atgaaggggg cttgtatcag acggcgtttg
      961 tggtggcgtt ttttgtgcca cgggtcgggg aagaatatgt ggatgccgtc gagcgagccg
     1021 tcttgcagca tattttcgac gacttctacg gcatcgtgcc gcatcacgcg gatattttcc
     1081 aaatggtttt cttctatgag tttgagcagg ttgcctacgc cgggaccgtg tacgtcgatg
     1141 gcgaggaagt cggtttcggg caggcggagg gcgatttctg cggttgctgt ccccataccg
     1201 aagcctattt cgaggatttt ggggcggttg cttccgaaat tggcatcaag atcggccggt
     1261 gttgcttggt agtcgatgcc gaagctgccc cataaggtat cgatggcgcg ttgctgcgcg
     1321 gcggtcatgt ggctttggcg gaggacgaaa ctgcggatgc tgcgggggtg ttcggcttgc
     1381 cagttgtttt gtgtttggtt ttcggcggtg tctgtcataa tgctttcgtg cgcttgtgca
     1441 atcaggatgc cgtctgaatg tgaaaggcct gttcggacgg cgggaaagga aaggggcgat
     1501 tatacctgtt tcgtttcggc agggtaggtg taacgctgtt cagacggcat tttgccattg
     1561 ttgcagcagt tcttgcagga cgcttttttg cccgtatccg ttgtttatgg cttcttgcag
     1621 gtagcggcag gctttttctt tgtccagccc gacaccgtgt ccgttgtaat ggatgcaggc
     1681 gaggttataa agtgccatgc tgtctttttg cgcggcggct ttttcaaacc atttgcgtgc
     1741 ttcattgtag tcggcggtca taccctgtcc gaagtaatac atgctgccca gattggtttg
     1801 ggctttgctg tggccgagtt ctgcggcttc tgtgtagagt ttgagtgcgg ctgcatattc
     1861 gggcgcgcat tgtaatccgt aatgcagggc ttgggctttt tgataaagcc gttctgccct
     1921 ttgccgcctg atggcggaat ctttaatgcc gccatattgg tcgggatggt ttaacgcgct
     1981 gtcggatatg agtttctgat acgcggcaag gctgccggct tcggctgcct gccgataata
     2041 atgccgggct ttttccgtat cgggggctat gcccaaactg tagcggcaga tgtcgcccag
     2101 tatccgcaaa ccttcgggat ggcgttcggc ggcggctgct tcggcgtgat gcagggcaag
     2161 tttgtagttt ctttccggat gtttgccttg caggtagatg tcggcaagtg cggcatgggc
     2221 atcggggtca ttttcctgtg ccgcctgttg aaaccagtga acggcttcat caggagccgt
     2281 aggtaggatg agctcggcaa gcggggtgta ggcgggaatg tatccttgtt gtgcggcggc
     2341 gcgcaaatga ttgacggcct gtgccgtgtc ggctttcgtc ccttgtccga aacggtattg
     2401 cagcccgagc tgccaatgtg cttccggcaa gccttgtgcc gccgcttttc ggtagagcag
     2461 gtgcgccgtt tcaaaatccg gcggcgtgcc gtagcggcag tattgggcaa gcaggtattg
     2521 cccgtggagg ttgttgtttt ttgcgcttgt ttttgcccat ttcagggctt cttcaaaatg
     2581 tcgggacgca tacagaaggt acatcaggcg gacttgtgct tcgggcagtc cttcctctgc
     2641 caacggtctg taaagcggta tcagggtttc cgggcgcgcg ccttccaatt cttggatata
     2701 tgccagattg taacgcgcgt agggatgtcc ctgttcggcg gcgatgcgga gcatttctat
     2761 gccctgtttg tagtcttggt aggggctgtt tgtatcaagc aggtgttttg ccaactcgaa
     2821 tgcggcggcg ggatgacctg ccgaggccgc ttctgtcagc agtcggcgga cttcggtaaa
     2881 gttatattgt ttttgagaca ggcgttctaa ggcctgccga tagggtaagt cgggattgtt
     2941 catatcagat cgttttccgt tttgtcgaat catcgttatt gtgttatgaa gttag
//
LOCUS       DAWKOG010000194         2954 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.194, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000194 DAWKOG010000000
VERSION     DAWKOG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.194"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(108..1418)
                     /locus_tag="WJK66_001950"
     CDS             complement(108..1418)
                     /locus_tag="WJK66_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEZ7579601.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIREAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     PLSSDDLKKLIAKVLALPEYQEFTIETDAQKLLVNTVDGDARRLLNLLEQLLRAADTR
                     RLKNLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRALQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRHFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLAWLKSLDEEVLKAK"
     gene            1730..2152
                     /locus_tag="WJK66_001951"
     CDS             1730..2152
                     /locus_tag="WJK66_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579602.1"
                     /translation="MEWVSLFSDPQKDDDSLITLKDEKITVKNYIVPWWKKGENFRKL
                     ELITTGKNDNGNLTVNGIYGIQSFADPQLGYTPELSVKSNNDILFKNNLQSVDEKPHS
                     GNIHLDAKGKIQFNLNVSDGDGVQDVASGKKYRQTIVP"
     gene            2239..2939
                     /locus_tag="WJK66_001952"
                     /pseudo
     CDS             2239..2939
                     /locus_tag="WJK66_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
BASE COUNT          813 a          713 c          714 g          714 t
ORIGIN      
        1 ttgtctttat tggctttatc gataaacagg ataagacctg aaaaaagccg atacgccttt
       61 ttggtgtacc ggctttgcca tactgttctg cttcagacgg catcgcttca ttttgccttt
      121 aatacttctt cgtccagcga tttcaaccac gccagctttt cgccgatttt gatttccagt
      181 ccgcgcggga cgggttggta gaaatcgggt tcgtccaggc cgtcgggcat atagctttcg
      241 ccggcggcgt aggcgttcgg ttcgtcgtgg gcgtagcggt attcgcgtcc gtagcccaat
      301 tccttcatca gcttggtcgg ggcgttgcgc aggtggacgg gcacttcgtc gctggcgttt
      361 tctttgacga agtggcgcat ttggttgtat gccttgtagc ccgcgttgga tttcgcggcg
      421 gcggcaagat acaataccgc ttgcgccaaa gccagttcgc cttcgggcga gcctaagcgt
      481 tcgaaggtgg cggcggcatc gttggcgatt tgcaaggcgc gcgggtcggc aagcccgatg
      541 tcttcccaag cgatacgcac gatgcggcgg gcgaggtagc gcgggtcggt gccgccgtcg
      601 agcatacggc agaaccaata cagcgcggcg ttcggatgcg aaccgcgcac ggatttgtgc
      661 agggcggaga tttggttgta gaaactctcg ccgcctttgt cgaaacggcg gatttgcgcc
      721 ccgagactgt cggcgagaaa ttcggcggtt aagtttttca gacgacgtgt atcggcggcg
      781 cgtaaaagtt gttccaacaa attcaacaat ctgcgcgcat caccgtcgac ggtattcacg
      841 agtaattttt gcgcatccgt ttcaatcgta aactcttggt attcaggcaa agccaatacc
      901 ttggcaatca gctttttcag gtcgtctgaa gacaagggtt gcaaaacata cacctgagcg
      961 cggctcaaca gcgcgggatt gacttcaaac gacggatttt ccgtcgtcgc accaataaag
     1021 gttagcaaac cgctttcgac atgcggcaaa aacgcgtcct gctgcgcctt gttgaagcgg
     1081 tggacttcat cgacaaacaa aatcgtcgcg cgtccctgct gcaaagcgat ttcggcttta
     1141 tcgattgcct cgcggatgtc cttcacgccg gaaaatacgg cggaaacagg caaaaactgg
     1201 gcgttgaaac tctgcgccaa aatccgcgcc aacgtcgtct tgcccacgcc cggcggcccc
     1261 cacagcaaca tagaatgcgg cttgccgcct tctaccgcca cgcgcaaagg tttaccttcg
     1321 ccgatgaggt gttcctgccc caccacgtcg tcaagcgtat gcggacgcaa tcgttcggca
     1381 agcggcgcgt cgggttctcg ggcaaacaaa tcggtcataa cggctccgtc aacaggtttt
     1441 caaacaatat gattatacgg cagggaacgg cggcgtgccg catacggatt ccgcccctcc
     1501 gtttgcctta agccgatatt aggcgcatac tggaaaagac gagagacttc acacaatata
     1561 tccggcacgg agaccgattc cgcaccggca tgacaatacc caaatcagcg tttcaattaa
     1621 acattaagga gactaaaata gaaaatttgc tttatctacc attgctttgt tgattcaatc
     1681 ggcattatgt tttgaggcgg aagcccatga atatactaat attcaagaga tggaatgggt
     1741 gtctttattt tctgatccgc aaaaagacga tgatagtctt ataaccctta aagatgaaaa
     1801 aatcactgta aaaaactata ttgtgccttg gtggaaaaaa ggtgaaaact ttagaaaatt
     1861 agaacttatt accactggta aaaatgataa tggaaattta acggtaaatg gaatttacgg
     1921 catccaatct tttgcagacc cgcaattagg atatacgcct gaattatcgg tgaaaagcaa
     1981 taatgatatt ctatttaaaa ataatctcca atctgttgac gagaagcccc attccggaaa
     2041 tatccatttg gatgccaaag gaaaaattca atttaatttg aatgtttccg atggtgatgg
     2101 ggtacaagat gtagccagtg gaaaaaaata ccgacaaaca atagtgcctt agtgcggtat
     2161 gtcaacacta tcagcccccc cgatgaaaat gacaaaggca gcatatcctt tcattcaaaa
     2221 acaggcaatt tgttcaatat gaacgttaac gttcataaag acacacacca tttcggtatt
     2281 cacaacctca acaataccga tgttcatctg actgccgaag acagcgataa tgtggttgcg
     2341 attaaagggg aactcgccga ccctatgccg acaggaaatg ttgagagtac aagtttttta
     2401 aggggcattg aaacggcttt taaatccaat accgtattaa cggggaataa caataaaata
     2461 tccatcgcca ctgagggcaa gggcttggga ttgggcgtaa atggtctcca gtccaactcc
     2521 ccctattccg gacacacgac gggcattttc atcaataatc aatcaagcga ccttacccaa
     2581 ccttcccttg atggcatcag caccatcaaa ctcaaagccg tcaagggaaa taatgaagtt
     2641 gatttaaatg taaaaaacca cgcctccgta aaaggcatta tcgcgtcgca ttctgcaaaa
     2701 gcaacgcttg aagccgaagg ggacaatatt gtccaggtta aaaatccgga caccaatacg
     2761 gctttaatcg atgctttgaa aaaacaatac cccaatgctt cgaaagaact atacagggtg
     2821 ggggttcaat ccacgactgg aacagtacgc atttcgccaa acggcaaaat cgcattaacg
     2881 gaattgaaaa cttttggaat tgtacaaaac ggcatttgcg tcagtttata gtggattaac
     2941 aaaaaccagt acgg
//
LOCUS       DAWKOG010000195         2947 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.195, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000195 DAWKOG010000000
VERSION     DAWKOG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.195"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            162..1274
                     /gene="nadA"
                     /locus_tag="WJK66_001953"
     CDS             162..1274
                     /gene="nadA"
                     /locus_tag="WJK66_001953"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEZ7579603.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDRETAGRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEASTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYICRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKHALTSGRNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
     gene            1326..2834
                     /gene="nadB"
                     /locus_tag="WJK66_001954"
     CDS             1326..2834
                     /gene="nadB"
                     /locus_tag="WJK66_001954"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEZ7579604.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASHH
                     AQGGIAAAWSREDDIEKHVADTLEAGAGLCNEAAVRTILSQGKPAIEWLLAQGVAFDR
                     NYDGLHLTREGGHTCRRIAHVADYTGEAVMQSLIAQIRRRPNIRVCERQMALDIQTES
                     GAACGLTVLDCRTQETHRIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     VENLEFIQFHPTGLARPSENGRTFLISEAVRGEGGILTNQAGERFMPHYDRRAELAPR
                     DIVARAIAAETAKQTQDFVSLDISHQPAAFVRQHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPSGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLAAQT
                     IADGQAFQTVPSKRSSENTSAEADIFSDGIQNTFSRPTLQAFNQRHLGILRNDTGLRR
                     AIAQLRLWKQNQTEPHTASEYENRNLLECSLAVAQAAYARRQNIGAHFNSDC"
BASE COUNT          691 a          907 c          814 g          535 t
ORIGIN      
        1 caaagcggtt tgtcggaacg aagcaggtcg gttttacggt atcgcaaacc tgttttcaga
       61 cgaccttgct tattatactc aagttgagca taatataaaa cagtttgaaa aacagacggc
      121 aaaccttcat gccgtctgaa aaccagctac aaggaaacat catgcaaacc gccgcccgcc
      181 gctcgttcga ttacgatatg cccctcatcc aaaccccgac ttccgcctgc caaatccgtc
      241 aggcgtgggc gaaggttgcc gacacgcccg accgcgagac ggcaggccgt ctgaaagacg
      301 aaatcaaggc tttgctgaag gagaaaaacg cggtcttggt ggcgcattat tacgtcgatc
      361 cgctgattca ggatttggct ttagaaacgg gcggatgcgt gggcgattcg ctggaaatgg
      421 cgcgcttcgg cgcggaacac gaagctagta cattggtggt ggcgggtgtg cgcttcatgg
      481 gcgagagcgc gaaaatcctc tgccctgaaa aaacagtgct gatgcctgat ttggaggcgg
      541 aatgttcttt ggatttgggc tgtccggaag aagcgttttc agcgttttgc gaccaacacc
      601 ccgaccgcac ggtcgtagtg tacgccaaca cttccgccgc cgtgaaagcg cgtgccgatt
      661 gggtggtaac gtcttcggtg gcgttggaaa tcgtgtcgta tctgaaatcg cgcggcgaga
      721 agctgatttg gggacccgac cgccacctcg gcgactacat ctgccgcgaa acgggcgcgg
      781 atatgctgtt gtggcagggt tcgtgcatcg tccataacga attcaaaggg caggagctgg
      841 cggcgttgaa agcggaacac cccgacgcgg tggtgctggt tcatcccgaa tcgccgcaaa
      901 gcgtcatcga actgggcgac gtggtcggct cgaccagcaa actgctcaaa gccgccgtat
      961 ctcgtcccga aaaaaaattc atcgtggcga ccgatttagg catcctgcac gaaatgcaaa
     1021 agcaggcgcc cgacaaagaa tttatcgccg cgccgacggc gggcaacggc ggaagctgca
     1081 aaagctgcgc gttctgcccg tggatggcga tgaattcgct gggcggcatc aaacacgccc
     1141 tgacaagcgg acgcaacgaa atcctgttgg acaggaagct gggcgaagcc gccaaactgc
     1201 ctttgcagcg tatgctcgac ttcgcggcag gactcaagcg cggggatgtg ttcaacggca
     1261 tggggcccgc ctgatttgcc gtcaatattg ccgtttcaga cgacctctca aacaaggaca
     1321 acaccatgca aaccgattgc gacgtattga ttgccggaaa cgggctggcg gcactgacgc
     1381 tcgccctgtc gctgcctgaa tcgttccgca tcgtcatttt gtgcaaaaac cggctggacg
     1441 acaccgccag ccatcatgcg caaggcggga ttgcggcggc gtggtcgaga gaggacgaca
     1501 tcgaaaaaca cgttgccgat actttggaag cgggcgcagg tttgtgcaac gaagccgccg
     1561 tccgcaccat cctgtcgcag ggcaaaccgg caatcgaatg gctgctggcg cagggcgtgg
     1621 cgttcgaccg aaattatgac ggtctgcacc tgacacgcga aggcgggcac acctgccgcc
     1681 gaatcgccca cgtcgccgac tacacgggcg aagccgtcat gcagagcctg attgcccaaa
     1741 tacgccgccg cccgaacatc cgcgtttgcg agcggcagat ggcgttggac attcaaaccg
     1801 aatcaggcgc ggcgtgcgga ctgaccgtcc tcgactgccg aacgcaagaa acccaccgca
     1861 tccgcgcccg ccataccgta ctcgcaggcg gcggcttggg gcagatttac gccgccacca
     1921 ccacgccgcc cgaatgcacg ggcgacgcca tcgctatggc gatacgcgca ggctgcgcag
     1981 tcgaaaacct cgaatttatc caattccacc ctacaggctt ggcaaggccg tctgaaaacg
     2041 gccgcacctt cctgatttcc gaagccgtgc gcggcgaagg cggcatcctg accaaccaag
     2101 cgggcgaacg gtttatgccg cattacgacc gccgcgccga actcgcaccg cgcgacatcg
     2161 ttgcccgcgc cattgccgcc gaaaccgcca aacaaacgca agacttcgtc tcgctcgaca
     2221 tcagccatca acccgcagcg ttcgtccgcc agcattttcc gtccatccat cgacactgtt
     2281 tatcccaatg cggcttggac atcacgcgcc aagccatccc cgtccgcccc gtgcaacact
     2341 acacctgcgg cggcatccaa accgacccca gcggcagaac ttccctgccg cagctctacg
     2401 ccttaggcga aaccgcctgc acagggttgc acggagccaa ccgcctcgcc agcaactccc
     2461 tactcgaatg cgtcgttacc gccaggcttg ccgcccaaac catcgcggat ggacaagcgt
     2521 tccaaaccgt accgtccaaa aggtcgtctg aaaacacctc cgccgaagca gacatctttt
     2581 cagacggcat ccaaaacaca ttcagccgcc ccaccctgca agcgttcaac caacgccatc
     2641 tgggcatcct ccgcaacgat accggcctgc gccgcgccat cgcccaactg cggctttgga
     2701 agcaaaacca aaccgagccg cacactgcgt ccgaatacga aaaccgcaac ctgctcgaat
     2761 gcagcctcgc cgtcgcccaa gccgcatacg caaggcggca aaacatcggc gcgcatttta
     2821 atagtgattg ttaaggttgg caggtcagag aaaatcgttc aaataaattt gaatagcatg
     2881 gttaagaaat tggcattcgg tttttagggt tgcgtaagtt gggttatcaa tctgttctaa
     2941 aagcaga
//
LOCUS       DAWKOG010000196         2881 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.196, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000196 DAWKOG010000000
VERSION     DAWKOG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.196"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            648..2819
                     /locus_tag="WJK66_001955"
     CDS             648..2819
                     /locus_tag="WJK66_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ7579605.1"
                     /translation="MNTPLFRLSLLSLTLAAGFAHAAENNAKVVLDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKEYEAGKGFRNFNGGKT
                     VPYSALDKRSYLAKIGTTFGDGDHRIVLSHMKDQHRGIRTVREEFTVGDASSRTNITR
                     QAPSYRETTQSNTNLAYTGKDLGFVEKLDANAYVLEKKRYSADDKDNGYAGNVVGPNH
                     TRITTRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNGEFEISGKKKDPKDPKKE
                     IDKTDEEKAKDKKDMDLVHSYKLSNPTKTDTGAYIEAIHEIDGFTLTGGLRYDRFKVK
                     THDGKTVSSSNLNPSFGVIWQPHEHWSFSSSHNYASRSPRLYDALQTHGKRGIISIAD
                     GTKAERARNTEIGFNYNDGTFAANGSYFWQTIKDALANPQNRHVSAAVREAVNAGYIK
                     NHGYELGASYRTGGLTAKVGVSHSKPRFYDTHPKKLLSANPEFGAQVGRTWTASLAYR
                     FQNPNLEIGWRGRYVQKAVGSILVAGQKDRNGKLENVVRKGFGVNDVFANWKPLGKDT
                     LNVNLSVNNVFNTFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
BASE COUNT          778 a          820 c          657 g          626 t
ORIGIN      
        1 cagtacagcg ttgcctcgcc ttagctcaaa gagaacgatt ctctaaggtg ctgaagcacc
       61 gagtgagtcg gttccgcact atccgtactg tctgcggctt cgccgccttg tcctgatttc
      121 cgttcgtttt cggttattcc cgataaatta ccgccgtttc tcgtcatttc tttaaccctt
      181 cgtcattccc gcgcaggcgg gaatctagtt ttttaagttc cagttgtttc tgataaattc
      241 ttgcagcttt gagttcctag attcccactt ttgcgggaag gacgcggaaa ggttgctttt
      301 cgtcattccc gcgcaggcgg gaatccagtc tgttcggttt cggttttttc cgatagattc
      361 ctgccgcgtt aggggttcta gattcccgct ttcgcgggaa ggacgcggaa aggttgcttt
      421 tcgtcattcc cgcatttgtt aatccactat atttccgccg ttttttacat ttccgacaaa
      481 acttgtcaac aaaaaacaac acttcgcaaa taaaaacgat aatcagcttt gcaaaaatcc
      541 ccccccctgt taatataaat aaaaataatt attaattatt tttcttatcc tgccaaacct
      601 taacggtttg gatttacttc ccttcataca ctcaagagga cgattgaatg aataccccat
      661 tgttccgtct cagcctgctc tcgcttaccc tggcggcagg ttttgcccac gcggcagaaa
      721 ataatgccaa ggtcgtactg gataccgtta ccgtaaaagg cgaccgccaa ggcagcaaaa
      781 tccgtaccaa catcgttacg ctgcaacaaa aagacgaaag caccgcaacc gatatgcgcg
      841 aactcttaaa agaagagcct tccatcgatt tcggcggcgg caacggcacg tcccaattcc
      901 tgacgctgcg cggcatgggt cagaactctg tcgacatcaa ggtggacaac gcctattccg
      961 acagccaaat cctttaccac caaggcagat ttattgtcga tcccgctttg gttaaagtcg
     1021 tttccgtaca aaaaggcgcg ggttctgcct ctgccggtat cggcgcaacc aacggcgcga
     1081 ttatcgccaa aaccgtcgat gcccaagacc tgctcaaagg cttggataaa aactggggcg
     1141 tgcgcctcaa cagcggcttt gccagcaacg aaggcgtaag ctacggcgcc agcgtattcg
     1201 gaaaagaggg caacttcgac ggtttgttct cttacaaccg caacgatgaa aaagaatatg
     1261 aagccggcaa aggcttccgt aatttcaacg gcggcaaaac cgtaccgtac agcgcactgg
     1321 acaaacgcag ctacctggcc aaaatcggaa caaccttcgg cgacggcgac caccgcatcg
     1381 tattgagcca catgaaagac cagcaccgag gcatccgtac cgtccgtgaa gaatttaccg
     1441 ttggcgacgc aagttcacgg acaaatatta cacgccaagc cccatcctac cgcgaaacca
     1501 cacaatccaa caccaacttg gcgtacaccg gcaaagattt gggctttgtc gaaaaactgg
     1561 atgccaacgc ctatgtgttg gaaaagaaac gctattccgc cgatgacaaa gataacggct
     1621 acgcaggcaa tgtggtcggc cctaaccaca cccgaatcac cactcggggt atgaacttca
     1681 actttgacag ccgccttgcc gaacaaaccc tgttgaaata cggtatcaac taccgccatc
     1741 aggaaatcaa accgcaagcg tttttgaacg gcgaatttga gatctccggt aagaagaaag
     1801 atccgaaaga tcccaaaaaa gaaatagata agaccgatga agaaaaagcg aaagacaaga
     1861 aagatatgga tcttgtccat tcctacaaac tgtccaaccc gaccaaaacc gataccggcg
     1921 cgtatatcga agccattcac gagattgacg gctttaccct gaccggcggg ctgcgttacg
     1981 accgcttcaa ggtgaaaacc cacgacggca aaaccgtttc aagcagcaac cttaacccga
     2041 gtttcggcgt gatttggcag ccgcacgaac actggagctt cagctcaagc cacaactacg
     2101 ccagccgcag cccgcgcctg tatgacgcgc tgcaaaccca cggcaaacgc ggcatcatct
     2161 cgattgccga cggcaccaaa gccgaacgcg cgcgcaatac cgaaatcggc ttcaactaca
     2221 acgacggcac gtttgccgcc aacggcagct acttctggca gaccatcaaa gacgcgcttg
     2281 ccaatccgca aaaccgccac gtctctgccg ccgtccgtga agccgtcaat gccggttaca
     2341 tcaaaaacca cggttacgaa ttgggcgcgt cctaccgcac cggcggcctg actgccaaag
     2401 tcggcgtaag ccacagcaaa ccgcgctttt acgataccca tcctaaaaaa ctgttgagcg
     2461 cgaatcctga atttggcgca caagtcggcc gcacttggac ggcttccctt gcctaccgct
     2521 tccaaaaccc gaatctggaa atcggctggc gcggccgtta tgttcaaaaa gccgtgggtt
     2581 cgatattggt ggcaggtcaa aaagaccgca acggcaaatt ggaaaacgtt gtacgcaaag
     2641 gtttcggtgt gaacgatgtc ttcgccaact ggaaaccgct gggcaaagac acgctcaatg
     2701 ttaatctttc ggttaacaac gtgttcaaca cgttctacta tccgcacagc caacgctgga
     2761 ccaataccct gccgggcgtg ggccgcgatg tacgcttggg cgtgaactac aagttctaaa
     2821 acgcacatcc cgaaaaaatg ccgtctgaaa gcctttcaga cggcatctgt tctgataatt
     2881 t
//
LOCUS       DAWKOG010000197         2593 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.197, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000197 DAWKOG010000000
VERSION     DAWKOG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.197"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(207..278)
                     /locus_tag="WJK66_001956"
     tRNA            complement(207..278)
                     /locus_tag="WJK66_001956"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(243..245),aa:Arg,seq:cct)
     gene            complement(298..744)
                     /locus_tag="WJK66_001957"
     CDS             complement(298..744)
                     /locus_tag="WJK66_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEZ7579606.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITISATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            complement(749..1723)
                     /gene="ispB"
                     /locus_tag="WJK66_001958"
     CDS             complement(749..1723)
                     /gene="ispB"
                     /locus_tag="WJK66_001958"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEZ7579607.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVTALDALPDSEVKDAMIQLAKESLVRVS"
     gene            complement(1739..1927)
                     /locus_tag="WJK66_001959"
     CDS             complement(1739..1927)
                     /locus_tag="WJK66_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579608.1"
                     /translation="MVNIRTPLCGTRHCIVICLFCQSLRGSDNHMPSEKMYPDGFADI
                     IARYLNQLSSGICRLTET"
     gene            1947..2255
                     /gene="rplU"
                     /locus_tag="WJK66_001960"
     CDS             1947..2255
                     /gene="rplU"
                     /locus_tag="WJK66_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEZ7579609.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            2280..2552
                     /gene="rpmA"
                     /locus_tag="WJK66_001961"
     CDS             2280..2552
                     /gene="rpmA"
                     /locus_tag="WJK66_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEZ7579610.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
BASE COUNT          760 a          699 c          575 g          559 t
ORIGIN      
        1 tccactatat cataaaacaa cagatttcca agccggaaca tcttttacgg agcctgaaaa
       61 tcatttcatg attctaccgt cctaaaggtc ggtttttcaa gcaggaagaa aaagttttca
      121 gatggcaaaa aagccctcca acactgaaag gccttatatc ggaaacttcc cgcaacacgg
      181 gaaacagaca aatgcaaatc gtcaaacctc gccaacagga atcgaacctg tattttacgc
      241 ttaggaggca tacgttctat ccgttgaact atggcgagcc gaaatgaaaa ggagatttta
      301 accctttccg acgacaaaag acaagatgcc ggccgcaatc agcggcccga cagggatacc
      361 gcccataaat gccaccccga taaccgtccc gattaacagc cctgtaatta aaacaggctg
      421 ctgtcccatc aaaggcacgc cgcgtcccgc cagccaagcc acgaaaatac cgataaaaac
      481 ggcggatatc attttaaaat tcaaaaattc ggcaacggga ggaacctgcg cctttcctga
      541 aaccaacgga ctcaaaaccc ctatggtcaa aagaatgata ccgagattca acccatgctt
      601 ctcgaccaac gggacaaact gtaccaatgc cgtctgctgc atcagcagca atatggttgc
      661 cgagatggta atcgaattgt tgttgctgac cacccccaac agaatcagcg taaccagaaa
      721 caggggaaca aaactgaaat tcatcgcctc aagacaccct gaccaaagat tccttcgcca
      781 gctgaatcat ggcatccttc acttcgctgt cgggcagggc atccaaggcg gtaacggcac
      841 aatcgactgc tttgcgcgcc tcgcctatcg aatatgccaa agcatccgaa cggacgacat
      901 aatcgtggat tttctcaaaa tagctgcgat ctgcattttc caaagcagta cgcacatcgt
      961 tcgcaacctg ttcggaaccc tgacgcatca gataaatcaa aggcaaagtc ggttttcctt
     1021 ccgccaaatc gtcgccgacg tttttgccgg tttcttcggt ttcgccagaa tagtccagca
     1081 catcgtcaat aatttggaat gccgtaccga catacatacc gtagtctttc aacgcccgtt
     1141 cgtgttcggg ggaagccttg cccaaaattg cgccgacttg agcggcagct tcaaacaatt
     1201 ttgccgtttt atattggatg acttggatat attgttcttc ggtaatgtcc gtattgccga
     1261 tgttcatcag ctgcatgact tcgccctcgg caataatgtt ggttgcatcc gccatcactt
     1321 ccaaaacgcg catactgccc gaggcaacca tcagttgaaa ggcgcgcgtg tataaaaagt
     1381 cgccaaccaa cacagccgcc gcattgccga acagattgtt tgccgttgcc cgcccacggc
     1441 gcaaatcgct ttcatcgacg acatcgtcgt gcaggagggt ggaagtgtgg ataaactcga
     1501 ccatcgccgc cagcgaatac agtttctcgt catcataacc gaccgcctta cccgccaaaa
     1561 tcgtcataat cggacgcagg cgtttgccgc ccgcgctgat gatatatgta ccgatttgcg
     1621 aaatcagtgc gacatcggat tgcaccgcac ggttgatgac ttcattgact ttggcaaggt
     1681 cttcaggcag atgtcgctgg aaatagggca gattctcgag catggcagac tctcaggttt
     1741 acgtttccgt caaacggcag ataccgcttg aaagctgatt caaatagcgg gcaattatat
     1801 cagcaaagcc atcagggtac atcttttcag acggcatatg gttatccgaa ccgcgcaaac
     1861 tttgacaaaa caggcaaata acaatacaat gccgagttcc gcacaatggt gtgcggatat
     1921 taaccataac ccttatggag ttgagtatgt acgcggtcgt aaaaaccggc ggcaaacagt
     1981 ataaagtttc cgtcggcgaa aaattgaaag tagaacagat accagccgaa ctcgacagcc
     2041 aaatcgaact gaccgaagtt ttgatgattg ctgacggcga atctgtaaaa gttggcgcac
     2101 cctttatcga aggtgcaaaa gtaacggcta aagtagtggc acacggtcgt ggcgaaaaag
     2161 tccgcatctt caaaatgcgc cgccgcaaac actaccaaaa acgccaaggc caccgccaaa
     2221 atttcaccca aatcgaaatc gtggcaatcg cctaacctta aaacttagga gcattacaaa
     2281 tggcaagtaa aaaagcaggc ggcagcaccc gcaacggtcg cgattcagaa gccaaacgct
     2341 tgggcgttaa agcctacggc aacgagctga ttcccgcagg ttccatcatc gtacgccaac
     2401 gcggtaccaa attccacgca ggcgacaacg taggtatggg caaagaccac actttgttcg
     2461 ccaaagttga cggttatgtc gaattcaaaa ccaaaggcgc gctgaaccgt aaaactgtca
     2521 gcatccgtcc ttacaccggt tctgaagaat aatccaaccg atacctgaag ccgcatcttt
     2581 tcacgatgcg gct
//
LOCUS       DAWKOG010000198         2583 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.198, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000198 DAWKOG010000000
VERSION     DAWKOG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.198"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(90..896)
                     /locus_tag="WJK66_001962"
     CDS             complement(90..896)
                     /locus_tag="WJK66_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HEZ7579611.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGRGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRNF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILNR"
     gene            1117..2487
                     /gene="ffh"
                     /locus_tag="WJK66_001963"
     CDS             1117..2487
                     /gene="ffh"
                     /locus_tag="WJK66_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEZ7579612.1"
                     /translation="MLDNLTGRFSNVLKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVVGSLTPDQAFIGVVNKALTELMGRENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTAGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMKGMK
                     GMFPGL"
BASE COUNT          704 a          655 c          672 g          552 t
ORIGIN      
        1 aatcccttcc tccccctcct ctgactggtg cgcctttgtg aatatgctgt ctgaaactcg
       61 gggcttcgga cggcatctgt tggctcttct tatctgttca gaatgatttc caatacgaac
      121 ttgctgccca tataggcaat catcaggctg acaaatccga tgatggtcca cacggcggct
      181 tttttgccgc gccatgcggt catgctgtgc ttgagcagca gtccgccgta aatcagccat
      241 gacaatatgc cgaatacggt tttatgggta aaggtcatgg gtttgccgaa tacggcttcg
      301 gcaaaaaatg ttccgctgac gacggaatag gtcagcagga tgaaacctgc ccacatggcc
      361 tggaacatga gtttttccaa actgagcagc gacggcagga atcctgcgag cttggagaag
      421 ttcctgcggt gcaggctccg attcagcagc agggtcaaaa cggacaataa tgttgcgatg
      481 ccgaacagcc cgtatgcaag cagcgaagtt ccgatatgca gcataaaggg aaggtcggta
      541 atttcatatc ccgagaattt tccaggaaaa accaaacctg acagcagcat cagtgcggcg
      601 caaggataca gcagcaactg cactccgcgc agcggataaa agaagctgcc ggcaaaataa
      661 ataaacagca tcatccaaac aatcaggctg ccggaatacc cgaagcccat aatgatgatt
      721 ttgtcttgaa tgaccggcat aagcagtgcc gcgccgtgga cggtcaatgc cgcgcccaaa
      781 accggcaatt ccgtcttcca cgggtaatcc cggccgcgcc cctgctgttg gcagtgccat
      841 gcaaatgcac ccaatcctgc gtaaaccgcc gtcaaaaaga tgaaaactgt cggcatggtg
      901 gactttctct aatctatact gttgcgctgt atgcggccgc ttatgaaata ttggaacttt
      961 taacgttgga attgtaaaat ccccgtttcg ggcaagcctt gacggatttg ccgatatgct
     1021 gtccggcaca caagccgcat caaattattt tgattttatt ttaacaaaga atgcccctga
     1081 tggggcaagc tattcttatt cagaccaagg accagtatgt tagacaattt aaccggccgc
     1141 ttcagcaatg ttctcaaaaa catccgcggg caggccaaac tgaccgaaga caatattaaa
     1201 gaagccttgc gcgaagtccg cctcgccctg cttgaggcgg atgtcgccct gcctgtcgtc
     1261 aaagagttcg tcaacaacgt caaagaaaag gccctcggtc aggaagtggt gggcagcctg
     1321 acgccggatc aggcatttat cggcgtggtc aacaaagccc tgaccgagct gatgggcagg
     1381 gaaaacaaaa cgctggattt gtcggttgcg ccgcccgccg tcgtgttgat ggcaggtttg
     1441 cagggcgcgg gcaagacgac gaccgccggc aaactcgccc gcctgctgaa aaacgatcag
     1501 aagaaaaagg ttttggtcgt atctgccgac gtttaccgcc ctgccgcgat tgaacaactg
     1561 cgtctgttgg ccgaacaggt cggcgtggac tttttcccat ccgataccaa ccaaaaaccg
     1621 gttgagattg caactgccgc cgtcgattac gccaaaaaac atttttacga tgtattgatg
     1681 gtcgataccg ccggccgttt ggcaatcgat gaggagatga tgaacgaaat caaagccctt
     1741 cacgcggcgg ttaacccggt ggaaactttg ttcgtcatcg atgcgatgct gggtcaggat
     1801 gcggtgaaca ctgctcaggc atttaatgaa gccctgccgc tgaccggtgt cgtattgacc
     1861 aagatggacg gcgattcgcg cggcggcgca gctttgtccg tgcgccacgt aaccggcaag
     1921 ccgattaaat ttatcggtgt cggcgaaaaa atcaacggcc tcgaaccttt ccaccccgac
     1981 cgtcttgcca gccgtatcct cggcatgggc gacgtattga ccctgattga agacgtccaa
     2041 aaaggtattg atgaagaagc cgccgccaaa atggcgaaaa agctacaaaa aggcaaaggc
     2101 ttcgacctca acgactttaa agaacaaatc cagcaaatgc gcaatatggg cggtttggaa
     2161 aacctgatgt cgaaaatgcc gggcgaactg ggtcaaatat cgaaacaaat ccccgaagga
     2221 acggctgaaa aagcgatggg caaagtggaa gccatcatca actcgatgac tccaaaagaa
     2281 cgcgccaacc ctgccctgct caaagccagc cgcaaacgcc gtattgcaat gggtgcgggc
     2341 acgaccgtac aggaagtgaa caaattgctc aaacagtttg aacaaatgca acaaatgatg
     2401 aagatgttca gcggcaacgg cttgggcaaa ctgatgcgta tggcgaaagg aatgaaggga
     2461 atgaaaggaa tgttcccggg tttgtaagcc gatttgaccg aaaacgccgt ctaaaatttc
     2521 agacggcgtt tttgttttat attctgattt atagtggatt aacaaaaatc aggacaaggc
     2581 ggc
//
LOCUS       DAWKOG010000199         2512 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.199, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000199 DAWKOG010000000
VERSION     DAWKOG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.199"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..1148)
                     /locus_tag="WJK66_001964"
     CDS             complement(<1..1148)
                     /locus_tag="WJK66_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ7579613.1"
                     /translation="MYSNNAITNNRNNITLGAYRDAIISIFSDENVVRAIGTINSMYE
                     EYLTRSHKIDQKEFLREGERLSAKAREFQRTAEEAQKSLKEGIGRAASKFRGNSKEAF
                     VARKVQSDLQKALNASNYNKQQYARRAATALENASKSKVMAANLGKFTYGATVAMGTL
                     SMGSAWWKALDGEGNWSDVGLSSASFLGGLMGVQWGAALGVGAFGLLTGGAGWLVVGA
                     ILGGGAGAWWGSLGAEKAWRWFEDFLNLNRSDKYHVYDPLALDLDGDGIETVATKGFA
                     GALFDHRNQGIRTATGWVAADDGLLVRDLNGNGIIDNGAELFGDNTKLADGSFAKHGY
                     AALAELDSNGDNIINAADAAFQSLRVWQDLNQDGISQANELRTLEELG"
     gene            complement(1705..2154)
                     /locus_tag="WJK66_001965"
     CDS             complement(1705..2154)
                     /locus_tag="WJK66_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579614.1"
                     /translation="MQPGSKNDEKNKITLNEFNNAIVTIFSDENVVRAIGTINTMHGE
                     YLTRSHKIDQQKFLSDGERLSSKAKDFQHSAEKTQRALKEGIGRAASKFHANSKEAFV
                     ARKMQSDLQKKLNASNYNKQQYARQAVTALENASKAENKGNIAFYLN"
BASE COUNT          798 a          546 c          376 g          792 t
ORIGIN      
        1 atacccaatt cttcaagggt acgcaattca ttagcttggg aaatgccgtc ctggttgaga
       61 tcctgccata cacgcaggga ttggaatgcg gcgtctgccg cgttgatgat gttgtcgccg
      121 tttgaatcca attcggccaa agccgcgtag ccgtgtttgg caaaagagcc gtctgccagt
      181 ttggtgttgt cgccgaagag ttccgcaccg ttgtcgatga tgccgttgcc gttcaaatcg
      241 cgcacaagca gaccgtcatc ggcagcaacc caacctgtgg cggtgcggat gccttgattg
      301 cggtggtcga acaatgcacc tgcaaagcct ttggtggcaa cggtttctat accgtcgccg
      361 tctagatcta gggcgagggg gtcgtaaacg tggtatttgt cgctgcggtt aagatttaag
      421 aaatcctcaa accatctcca agctttttca gctcctaatg atccccacca agctcctgcg
      481 ccacctccaa gtatagctcc aactactagc caacctgcgc caccagtaag taaaccaaaa
      541 gccccaacac ccaaagccgc accccattgg acgcccatta gcccacccaa aaaagaagct
      601 gatgagagac caacatcact ccaatttcct tctccatcca aggctttcca ccaagcactt
      661 cccatactca aagttcccat ggcaacagtt gccccataag taaacttacc caaattcgct
      721 gccataactt ttgattttga agcattctct aacgctgttg ccgcacgtct tgcatattgc
      781 tgcttgttat agttgctagc attcaatgct ttttgcaaat cactttgtac ttttcttgca
      841 acaaatgcct cctttgaatt accacgaaat ttactagcag ctcttccaat tccttctttt
      901 aagctttttt gtgcctcttc tgctgtgcgt tggaattctc ttgccttagc agacagacgc
      961 tcaccctccc ttaaaaattc tttttgatct atcttgtgcg aacgggtcaa atattcttcg
     1021 tacattgagt taatcgtacc aatcgcccta accacatttt catcagaaaa tatggaaata
     1081 atagcatccc tatacgcacc tagtgtaata ttgtttctat tattagttat agcattattc
     1141 gaatacataa tagcacctcc aaatttagaa ctaaatacgt ttatattctc tgtgtaataa
     1201 tatcaaataa ttaattaatg gataagtaag tccgaaaaaa taacaactaa aataagcaat
     1261 atactgattc tctcctttat taattagata tattgaaaaa caaataaaaa ataaacagaa
     1321 aacattcatg tgaaaaagcc acctcatatt cttatttata agtttaaaat aactaggcat
     1381 tgtatattta aaaatcggca catccaacgg aaacaaacca cataaaaatt tttctttatt
     1441 ctttattaaa gacatagttt tattttttaa aattagtcca aataatatac taacaattac
     1501 tattaaaaca ataagcacag cgtaattttt tataaaattc attaaaataa taacgaaaat
     1561 actaaataga ttacatacta taaattgaaa tataaaatat tcagtattta atccattttc
     1621 aaccgcgagt tttttaaaat atccttgctc gaaaaaatta aaaaaatcca ttaatacttt
     1681 cctctcaaga aaactaattt tagtttagtt aagataaaat gcaatattac ctttattttc
     1741 tgcttttgaa gcattttcca aagctgtgac tgcttgtctt gcatattgtt gcttgttata
     1801 gttactagca tttaacttct tttgcaaatc actttgcatt tttcttgcaa caaatgcctc
     1861 cttcgaattg gcatgaaatt tactagcagc ccttccaatc ccttctttta aagccctttg
     1921 agttttttct gctgagtgct gaaaatcttt cgctttacta gacaaccgct caccatcact
     1981 caaaaatttt tgttgatcta ttttatgcga acgagttaaa tactccccat gcattgtgtt
     2041 aattgtaccg attgccctaa ccacattttc atcagagaaa atagttacaa tggcattatt
     2101 gaattcattt agtgtaatct tatttttttc atcattctta gaaccgggtt gcatgacaac
     2161 accttccatc aactaaaata ttatattatt aagagactac aacttagata cgtttataat
     2221 ctctggataa aaaaacgtaa taagtgtttg ctggataaca aagtccaaac caatagcaga
     2281 taaaataagg catccacccc ctttcttcat taaggatcag agatatcgag aaacaaatca
     2341 taactaaaca gaaaaaattt tggagataaa gccatttcat tcccctattc aagaatctgg
     2401 ccaagatagg tattttgtat actctagaaa agaaaggcat ttccaaggga aacatatcag
     2461 ataaaaactt ttgtttattt tttactatag atagaaccct atttctcaag at
//
LOCUS       DAWKOG010000200         2496 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.200, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000200 DAWKOG010000000
VERSION     DAWKOG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.200"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            168..791
                     /gene="lolA"
                     /locus_tag="WJK66_001966"
     CDS             168..791
                     /gene="lolA"
                     /locus_tag="WJK66_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEZ7579615.1"
                     /translation="MMKPHNLFQFLAVCSLTVSVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTSPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            1064..2203
                     /locus_tag="WJK66_001967"
     CDS             1064..2203
                     /locus_tag="WJK66_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7579616.1"
                     /translation="MKKTLVAAAILSLALTACGGGSDTAAQTPSAKPEAGQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTQQVKKAL
                     GTDKLPENEWDLVFKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARDLM
                     EAKYTNDPSIFPTKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK"
     gene            2335..>2496
                     /locus_tag="WJK66_001968"
     CDS             2335..>2496
                     /locus_tag="WJK66_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7579617.1"
                     /translation="MDLSIVVPIYNVESYLEACLNSIEPILSNENVELILVNDGSKDG
                     SEDICYKYID"
BASE COUNT          676 a          722 c          616 g          482 t
ORIGIN      
        1 gcggcacaca attaaagggc agcgtggcgc gccgcctttt ccggtgggca aaaaatcagc
       61 cctcggaaaa cgcggtttgc aaaatgcaaa ccgcccgtaa cgccgcccat atgattgttt
      121 tgccgcgccg atactttacg ccacacttat tccaacaagg aaaaataatg atgaaaccgc
      181 acaacctgtt ccaattcctc gccgtttgct ccctgaccgt ctccgtcgct tccgcacagg
      241 cgggcgcggt ggacgcgctc aagcaattca acaacgatgc cgacggtatc agcggcagct
      301 tcacccaaac cgtccaaagc aaaaagaaaa cccaaaccgc gcacggcacg ttcaaaatcc
      361 tgcgcccggg cctcttcaaa tgggaataca cttcgcccta caaacagact attgtcggcg
      421 acggtcaaac cgtttggctc tacgatgttg atttggcaca agtgaccaag tcgtcccaag
      481 accaggccat aggcggcagc cccgccgcca tcctgtcgaa caaaaccgcc ctcgaaagca
      541 gctacacgct gaaagaggac ggttcgtcca acggcatcga ttatgtgctg gcaacgccca
      601 aacgcaacaa tgccggctac caatacatcc gcatcggctt caaaggcggc aacctcgccg
      661 ccatgcagct taaagacagc ttcggcaacc aaacctccat cagtttcggc ggtttgaata
      721 ccaatcccca actctcgcgc ggcgcgttca agtttacccc gcccaaaggc gtggacgtgt
      781 tgagcaactg atgccgtccg cctcgatgcc gtctgaatgc cgccgaggct tcagacggca
      841 ttttcacgca ggcggaacaa tgtcccgcat taccgcccga tcgggcatcg gaacggcaaa
      901 cccgtgaaaa ttaacggttg cgccccggtt gtttttgccg tttaatgcaa accttgctgc
      961 accaagggcc aagaaagccg accggccgcc cccacagctt ccgatgcagg cggcccgtcc
     1021 gtccctgcaa tgttttttat ttttgaacga aaggtcgaaa accatgaaaa aaacactggt
     1081 ggcggcggcg atcctgagcc tcgccttgac tgcgtgcggc ggcggaagcg ataccgccgc
     1141 ccaaaccccc tccgccaagc ccgaagccgg acaatcgggc aaactcaata tctacaactg
     1201 gtcggattat gtcgatcccg aaaccgtcgc cgcctttgaa aaagaaaccg gcatcaagac
     1261 gcgttccgat tattacgaca gcaacgaaac actggaggca aaagtcctga ccggcaaatc
     1321 cggctacgac ctgaccgcgc cgtccatcgc caacgtcggc cggcaaatca aagcgggtgc
     1381 gtatcagaaa atcgacaagg cgcaaatccc ccattacggc aacatcgata aagatttgct
     1441 gaaaatgatg gaagccgtcg atccgggcaa cgaatatgcc gtgccttatt tctggggaat
     1501 caatactttg gcaatcaata cccagcaggt gaaaaaggca ttgggtacgg acaagctacc
     1561 cgaaaacgag tgggatttgg tgttcaaacc cgaatacacc gccaaactca aatcctgcgg
     1621 catcagctat ttcgacagcg caatcgaaca gattcccttg gcgttgcact atttgggcaa
     1681 agaccccaac agtgaaaatc ctgaagacat caaagccgcc gtcgatatga tgaaagccgt
     1741 ccggggcgac gtgaaacgct tcagctcttc cggctatatc gacgatatgg cggcgggcaa
     1801 cctgtgtgcc gccattggtt acggcggcga tttgaacatt gccaaaaccc gtgccgaaga
     1861 agccgcaaac ggcgtggaaa tcaaagtatt gaccccgaaa accggcgtgg gcgtgtgggt
     1921 ggattccttt atgattccgc gcgacgcgca aaacgttgcc aacgcccacc gctatatcga
     1981 ctacacgctc cggcccgagg tggcggcgaa aaacggcagc ttcgtcacct acgcgcccgc
     2041 cagccgcccc gcgcgtgatt tgatggaagc caaatacaca aacgacccat cgattttccc
     2101 gaccaaagaa ctgatggaaa aaagtttcat cgtatcgccc aaatccgcag aatccgccaa
     2161 actgggcgtg aagctgtggc aagggctcaa ggccggaaaa taaccgcaac ctctgccgtc
     2221 tgaaaccttt cgggcggcag gaaacggcgc gtccgttatc aaacaggggg cgtttccccg
     2281 cccgcctgtt atgattgggt taagattaaa atgatttagt aaaatgagaa agatatggat
     2341 ttaagtatcg tagttcccat ttataatgtc gagagttatt tggaagcgtg tctaaattcc
     2401 atagaaccta tattaagtaa tgaaaatgtc gaacttatcc ttgtgaatga cgggtcaaaa
     2461 gacggaagtg aagatatatg ttataaatat atagat
//
LOCUS       DAWKOG010000201         2458 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.201, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000201 DAWKOG010000000
VERSION     DAWKOG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.201"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            35..1390
                     /locus_tag="WJK66_001969"
     CDS             35..1390
                     /locus_tag="WJK66_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HEZ7579618.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHNLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLLYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKGY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLVITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWFELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMLGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
     gene            1394..2404
                     /locus_tag="WJK66_001970"
     CDS             1394..2404
                     /locus_tag="WJK66_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEZ7579619.1"
                     /translation="MSDTPATRDFGLIDGRAVTGYVLSNRRGTRVCVLDLGGIVQEFS
                     VLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEANEGR
                     NALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDRLTV
                     SYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVSDDLEVF
                     DFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRRRISIYSDRNGL
                     VIFTAAPQDFARHDAGVYDALATEAQTLPDSLNWPEFGNIRLNKGDTREATIAYGIES
                     LS"
BASE COUNT          453 a          660 c          740 g          605 t
ORIGIN      
        1 actatacttt tcaaatcaaa aaaggattta ccttatgtcg gaatatacgc ctcaaacagc
       61 aaaacaaggt ttgcccgcgc tggcaaaaag cacgatttgg atgctcagtt tcggctttct
      121 cggcgttcag acggccttta ccctgcaaag ctcgcaaatg agccgcattt ttcaaacgct
      181 aggcgcagac ccgcacaatt tgggctggtt tttcatcctg ccgccgctgg cggggatgct
      241 ggtgcagccg attgtcggcc attactccga ccgcacttgg aagccgcgtt tgggcggccg
      301 ccgtctgccg tatctgcttt atggcacgct gattgcggtt attgtgatga ttttgatgcc
      361 gaactcgggc agcttcggtt tcggctatgc gtcgctggcg gctttgtcgt tcggcgcgct
      421 gatgattgcg ctgttggacg tgtcgtcaaa tatggcgatg cagccgttta agatgatggt
      481 cggcgacatg gtcaacgagg agcagaaagg ctacgcctac gggattcaaa gtttcttagc
      541 gaatacgggc gcggtcgtgg cggcgattct gccgtttgtg tttgcgtata tcggtttggc
      601 gaacaccgcc gagaaaggcg ttgtgccgca gaccgtggtc gtggcgtttt atgtgggtgc
      661 ggcgttgctg gtgattacca gcgcgttcac gattttcaaa gtgaaggaat acgatccgga
      721 aacctacgcc cgttaccacg gcatcgatgt cgccgcgaat caggaaaaag ccaactggtt
      781 cgaactctta aaaaccgcgc ctaaagcgtt ttggacggtt actctggtac agtttttctg
      841 ctggttcgcc ttccaatata tgtggactta ctcggcaggc gcgattgcgg aaaacgtctg
      901 gcacaccacc gatgcgtctt ccgtaggtta tcaggaggcg ggcaactggt acggcgtttt
      961 ggcggcggtg cagtcggttg cggcggtgat ttgttcgttt gtattggcga aagtgccgaa
     1021 taaataccat aaggcgggtt atttcggctg tttggctttg ggcgcgctcg gctttttctc
     1081 cgttttcttc atcggcaacc aatacgcgct ggtgttgtct tataccttaa tcggcatcgc
     1141 ttgggcgggc attatcactt atccgctgac gattgtaacc aacgccttgt cgggcaagca
     1201 tatgggcact tacttgggcc tgtttaacgg ctctatctgt atgcctcaaa tcgtcgcttc
     1261 gctgttgagt ttcgtgcttt tccctatgct gggcggcttg caggccacta tgttcttggt
     1321 agggggcgtc gtcctgctgc tgggcgcgtt ttctgtgttc ctgattaaag aaacacacgg
     1381 cggggtttga gcgatgagcg atacccccgc tacccgcgat tttggcctga tcgacgggcg
     1441 tgccgtaacc ggctatgtgc tgtccaaccg gcgtggtacg cgtgtctgcg tgctggactt
     1501 gggcgggatt gtgcaggaat tttccgtttt ggcagacggc gtgcgcgaaa acctcgtggt
     1561 gtcgttcgac gatgcggctt cctatgcgga caatccgttt cagattaaca agcagatagg
     1621 gcgcgtggcc ggacgcatcc gcggtgcggc gttcgacatc aacggcagga cttaccgcgt
     1681 ggaggccaac gaaggcagga acgcgctgca cggcggttcg cacgggctgg ccgttacccg
     1741 tttcaacgcg gtggcggcag acggccgttc ggtggtgctg cgcagccgcc tgcaacagtc
     1801 ggccgacggt tatcccaacg atttggattt ggatatttcc taccgcttgg acgaggacga
     1861 ccggcttacc gttagctatc gcgccaccgc gctcggcgac acggtgttcg acccgacgct
     1921 gcacatttac tggcggctgg acgcgggcct gcacgatgcg gttctgcata ttccgcaggg
     1981 cggacatatt ccggccgatg ccgaaaaact gcccgtctca acggtttcag acgacctcga
     2041 agtatttgat ttcagccggc ccaagccgct ggatgccgcc gttgccgccc tgcgccgcga
     2101 aacgggtcgg gccggttttg acgacgctta ccgcgtgccg tccgatatag gccgtcccgc
     2161 cgctgtgttg caagccggac gccgccgtcg tatcagcata tacagcgacc gcaatggctt
     2221 ggtcatcttt accgccgccc cgcaggattt cgcgcggcac gatgcgggcg tttacgacgc
     2281 gctggcgacc gaggcgcaga cgctgcccga cagcctgaat tggcccgagt tcggcaatat
     2341 tcgtctgaac aagggtgata ccagggaggc gacgattgct tacggcatcg aatccctttc
     2401 ttaggagctt cctaacaccg gttgtagacg gcctttttat agtggattaa caaaaacc
//
LOCUS       DAWKOG010000202         2442 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.202, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000202 DAWKOG010000000
VERSION     DAWKOG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.202"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(13..1215)
                     /gene="trpB"
                     /locus_tag="WJK66_001971"
     CDS             complement(13..1215)
                     /gene="trpB"
                     /locus_tag="WJK66_001971"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEZ7579620.1"
                     /translation="MKNYHAPDEKGFFGEHGGLFVSETLIPALQELADAYKAAKNDPE
                     FWEAFRHDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVESGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     AQMQEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(1400..2089)
                     /locus_tag="WJK66_001972"
     CDS             complement(1400..2089)
                     /locus_tag="WJK66_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HEZ7579621.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDSVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFNDAAHFQRLSEKAFDAE
                     KIKAFLHERGIASCPTVLKAAREHGNASDKFLKVVETVDLAAVLAKIPDCRHICTTGG
                     KATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAAYRAF
                     FEMAGLCEKQL"
     gene            2286..>2442
                     /locus_tag="WJK66_001973"
     CDS             2286..>2442
                     /locus_tag="WJK66_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase"
                     /protein_id="HEZ7579622.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAK"
BASE COUNT          538 a          706 c          631 g          567 t
ORIGIN      
        1 agacggacga ggttacagtt taatcccttt gagcttcgcg acggtattga tgtctttgtc
       61 gccacgaccg gataggttga ccagaatcac ttggtctttg cccattttcg gcgcgttttt
      121 caccgcccaa gcaacggcgt ggctggattc gagcgcgggg atgatgccct cgaagcggca
      181 gagcaagtca aaggcttcga gtgcttcgtc gtctttggca acagtgtatt cgacgcgctt
      241 gatgtcgtgc agatggctgt gttccgggcc gatgccgggg taatccaagc ctgcggaaac
      301 agagtgcgta cccaaaacct gaccgttttc gtcctgcatc agatagctgc ggaaaccgtg
      361 caatacgcca atcggtgcgc ccgaagtaat cggcgcggcg tgatcggggg tgttcacgcc
      421 caaaccgcca gcctccacgc cgacgaggcg cacgttttct tcgccgatat acgggtggaa
      481 caaaccgatg gcgttcgatc cgccgcccac gcaggcaacg gcaacgtcgg gctgtctgcc
      541 gatggcttcc tgcatctgcg ctttagcttc gttgccaatc acgcattgga aatcgcgcac
      601 catttcggga tacggcgcgg ggccggcggc ggtaccgatg atgtagaacg tgtcgtccac
      661 gcgggcgacc cattcgcgca tggcttcgtt catcgcgtct ttcagcgtgc ggctgccgct
      721 ttctacaccg accacgttcg cacccaataa tttcatacgg aacacgttgg gcatttggcg
      781 ttggatgtcg tccgcgccca tatacacgtc gcaagtcata ccgaagcgtg cggcaacggt
      841 ggcactcgcc acgccgtgct gacccgcgcc ggtttcggcg atgacgcgtt ttttacccat
      901 gcgttttgcc aacagtgcct gaccgatggt gttgttgact ttgtgcgcgc cggtgtggtt
      961 caagtcttcg cgcttcaacc agatttgcgc gccgcccaga tgttcggaca accgcgcggc
     1021 gtggtaaacg gggctgggcc tgccgacata atgtttcaaa tcatggcgga acgcttccca
     1081 aaattcagga tcgtttttcg ctgccttata ggcatccgcc agctcttgca aagccggaat
     1141 cagggtctcg gacacgaaca gcccgccgtg ttcgccgaaa aagcccttct cgtcgggcgc
     1201 gtggtagttc ttcatcagat ttcgtccttg tgcttcaaat tttcagacgg caatttatag
     1261 tggattaaca aaaaccggta cggcgttgcc ccgccttgcc gtactatttg tactgtctgc
     1321 ggcttcgccg ccttgtcctg atttttgtta atccactata tttttcaaac atgccgtccg
     1381 aacgggaaat tgtcggcaat tataactgtt tttcacacaa gcccgccatt tcaaaaaacg
     1441 cccgatacgc cgccgctttt ttcgccaaac tcaaaggata ggcacgcgaa gtcgaaggca
     1501 ggcgcgtcag cgtcaaatcc cgtccggcaa acggaaacgg cacggtttcg cccgttttcg
     1561 gcattttgat gccgccaccc tgaatatcga gcaggatttc cgtcgccttg ccgcctgtcg
     1621 tacaaatatg gcggcagtct ggtattttcg ccaacaccgc cgccaaatcg acggtttcga
     1681 ctacctttaa aaacttgtcg gacgcattgc cgtgttccct tgccgccttc aacacggtcg
     1741 gacaggacgc aatcccccgt tcgtgcaaaa acgccttgat tttctcggca tcaaacgctt
     1801 tttcagacaa cctttggaaa tgcgccgcat cattaaaaaa caccagcccg taaacgcgcc
     1861 acatatcgtt ttggaaattc ggataatgaa actgcatcgc gcgtttgtct tccttgggcg
     1921 gaaacgtccc catcatcatg accgcagcct tcggcggcaa cacggaatcg aagggatggg
     1981 tttcgatttc aagcagattt tcagacacgt tcaatcctcc aacaggcaaa acggcagaca
     2041 gccggttttt gcaccaaacc gccaaacacg gcaagccttc aaacagcatt atcacgccct
     2101 gccgttttcg gttacaatgc cgacatcaaa cggatactgt aaacacattt atgttccaac
     2161 acacaggacg acacttaaag caccgcccta tgtgccatcc tgatttggaa ggagttacgc
     2221 ccctcccaaa tagagtctga ttctaccgcc ctaaagggcg gggtttcaac cgaaaaggaa
     2281 acacgatgaa agaacacaaa gcccgcaagc gtttcgggca gaattttttg caggacacgc
     2341 ggatcatcag cgatattgtc aacgccgtgc gcccgcaggc ggatgatgtc gtgattgaaa
     2401 tcggcccggg tttggcggcg attaccgaac ctttggcgaa aa
//
LOCUS       DAWKOG010000203         2417 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.203, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000203 DAWKOG010000000
VERSION     DAWKOG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.203"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..197)
                     /locus_tag="WJK66_001974"
     CDS             complement(<1..197)
                     /locus_tag="WJK66_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579623.1"
                     /translation="MNIMNTCRKYGAKLAVLTAAPLALAAHANATLPDTAKNALEAAK
                     ADGMEAGWIVVGIFAALFVFS"
     gene            complement(283..486)
                     /locus_tag="WJK66_001975"
     CDS             complement(283..486)
                     /locus_tag="WJK66_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579624.1"
                     /translation="MSRYQQKFIVQELENHEFIYPDPFGDIGFTPNIKSAGKYDSYED
                     AFSSALEEIGGEFVIFGFYEKED"
     gene            complement(500..811)
                     /locus_tag="WJK66_001976"
     CDS             complement(500..811)
                     /locus_tag="WJK66_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579625.1"
                     /translation="MFETSQVTTYPATLLGAKQFKGEIDGNKIDSCTVLVASPMSSNG
                     NAVGFTAASMKFGDSHNFEKLKNLKFPCAVDVTVAMESTGKGLVPKLLDFQVKGAAPK
                     A"
     gene            complement(825..2168)
                     /locus_tag="WJK66_001977"
     CDS             complement(825..2168)
                     /locus_tag="WJK66_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ7579626.1"
                     /translation="MKAFEDKALAVSADMADALADGGAAATAVGCPPRLIGGEQNKTP
                     NPKGAEKTENQEFQFEYFSHFVSDGKGRFIEIPLRRGRDDGAFIDQITFTIHENSMTK
                     VTGKGLVSDTEFVVQYSELLEEILGFGITRKLPFKGKFFYQSCYQLGPDNVEYGKVHY
                     GGQRETMLVELNGTGCMAALPGWENRLHEFLSKCVRPKITRVDVAHDFFNGEYTPDQA
                     MLDHDNGHYDVHNMRPKSECRGTAWRNEDGSGKTFYIGKRGNSKFTRVYEKGRQFGDV
                     DSPWVRFETEFRAGDIEIPLDILLYSGSYLGGAYPICKAIFKTEAKRMEVKVKNVNLI
                     FDVKLFHARNQVGKMVNFLRDIGWDDSRIVDELVKGVEGYPKGLQPEQYDCKNQTQKF
                     QYIHEEQKAINALNIETLFDDLIEEREYAFPQDRERQHIKDIEYEERQISDFLNN"
     gene            complement(2155..2379)
                     /locus_tag="WJK66_001978"
     CDS             complement(2155..2379)
                     /locus_tag="WJK66_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579627.1"
                     /translation="MFIFYTVNPEHVYFPKAYIMKVFKDKGYESQCITTVSFYICNPT
                     LKQKTENEAYEYGRLFVKELMDKECNRESL"
BASE COUNT          574 a          580 c          491 g          772 t
ORIGIN      
        1 atggaaaaca caaaaagcgc ggcgaaaatg cccactacaa tccaaccggc ttccataccg
       61 tcggcttttg cggcttccaa agcgtttttt gctgtatcgg gcaacgttgc atttgcatgt
      121 gcggccaaag ccaggggagc agctgttaga acagccagtt ttgcgccgta tttgcggcag
      181 gtgttcatga tgttcatgat attttccttt acgaaatttt taaaaaaatg tgtttgcggg
      241 ctttgtgaag gttttagaga ccgcccgccg agcctcttaa acttaatctt ctttttcgta
      301 gaatccgaaa attacaaatt ccccgcctat ctcttccaat gccgagctaa aagcgtcttc
      361 atagctgtca tatttaccgg ctgatttgat gttcggcgta aagccaatat cgccaaacgg
      421 atcgggatag atgaattcgt gattttccaa ttcctgtacg ataaatttct gttggtatct
      481 gctcatgatt cagcctttct taggctttag gtgctgcgcc ctttacttgg aaatccaaca
      541 atttcggcac taagcctttt cctgttgatt ccattgcaac ggttacatca accgcacagg
      601 ggaatttaag gtttttcaat ttttcaaaat tatggctgtc accaaacttc atgcttgccg
      661 cggtaaagcc cacggcattg ccgtttgacg acatggggct agctaccaag acggtgcatg
      721 aatcgatttt gttaccgtcg atttcgcctt tgaattgttt tgcacccaac aaagtggccg
      781 gatatgtggt tacttggctt gtttcaaaca tttttcaatt tccttcagtt gtttaaaaaa
      841 tcggatattt gacgttcctc gtactcaata tccttgatgt gttgccgttc cctgtcttga
      901 ggaaatgcgt attctctctc ttcaatcaaa tcatcaaaca gtgtttcaat gttcaatgcg
      961 ttaatggctt tctgttcttc gtggatatac tggaatttct gcgtttggtt tttgcaatcg
     1021 tattgctcag gctgtaaacc ttttggataa ccttcaacgc ctttcaccag ctcatcaact
     1081 atcctgctat cgtcccaccc tatatcgcgg aggaaattaa ccatcttgcc gacttgattc
     1141 cttgcatgaa aaagcttaac gtcgaatatc agattaacgt ttttaacttt tacttccatg
     1201 cgcttggctt ctgtcttgaa tatcgcctta caaatcggat atgcgccgcc tagatacgac
     1261 cccgaataaa gtaggatatc caagggtatt tctatatccc ccgccctgaa ttctgtttca
     1321 aacctgaccc aagggctatc aacatctcca aactgcctgc ctttctcgta aacacgggta
     1381 aattttgaat ttccgcgctt gccgatgtaa aaggtttttc cactgccgtc ttcattgcgc
     1441 catgccgtgc cgcgacattc gctttttggc cgcatattgt gtacgtcgta atgtccgtta
     1501 tcgtggtcta acattgcctg atcgggtgtg tactctccgt tgaaaaaatc atgggctaca
     1561 tcgacacggg ttattttcgg gcggacgcat ttacttaaga attcatgtag gcggttttcc
     1621 caaccgggta aagcagccat gcagcctgta ccgttcaatt caaccaacat cgtttcacgt
     1681 tgaccgccgt aatgaacctt tccgtattca acgttatcgg gcccgagttg ataacagctt
     1741 tgatagaaaa actttccttt aaacggtaat tttctggtaa tgccgaatcc caagatttct
     1801 tcaagcagct cgctatactg gacaacgaat tccgtatctg atactaagcc ctttccggtt
     1861 actttcgtca tggaattttc atgtatcgtg aaagtgattt ggtctatgaa cgctccgtca
     1921 tccctgccac gccttaacgg tatttctata aatctgcctt tgccgtccga tacaaaatga
     1981 ctgaaatact cgaattgaaa ttcttggttt tctgtttttt ccgcaccctt cggatttggg
     2041 gttttatttt gctccccccc tattagccta ggggggcagc ctacggcggt tgccgcagcc
     2101 ccgccgtccg ctaacgcgtc cgccatgtcc gcggacaccg ccaaggcttt atcttcaaag
     2161 gctttcacgg ttgcattcct tatccatcag ttctttaaca aacaatctgc catattcata
     2221 cgcttcgttt tcggtctttt gtttcaaagt cggattgcag atataaaaag aaactgttgt
     2281 gatgcattgg gattcgtagc ctttatcctt gaacactttc atgatgtatg ctttggggaa
     2341 atatacgtgt tcgggattga cagtatagaa aataaacatg attaagcccc ttcactggct
     2401 agagttaagg ggcttta
//
LOCUS       DAWKOG010000204         2389 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.204, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000204 DAWKOG010000000
VERSION     DAWKOG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.204"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            77..2227
                     /gene="yccS"
                     /locus_tag="WJK66_001979"
     CDS             77..2227
                     /gene="yccS"
                     /locus_tag="WJK66_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HEZ7579628.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIITTVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYDALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDSNDNPDIRHLRRLLDNLGS
                     VDQQFRQLRHSDSPAENDRMGDTRIAALETGSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIVSTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKITERLKSGETGDDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALDTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT          567 a          927 c          478 g          417 t
ORIGIN      
        1 agctaaggcg aggcaacgcc gtactggttt ttgttaatcc actatattca cttcataaca
       61 ataaaccggt aaaaccatga aaaccccact cctcaagcct ctgctcatta cctcgcttcc
      121 cgttttcgcc agtgttttta ccgccgcctc catcgtctgg cagctaggcg aacccaagct
      181 cgccatgccc ttcgtactcg gcatcatcgc cggcggcctt gtcgatttgg acaaccgcct
      241 gaccggacgg ctgaaaaaca tcatcaccac cgtcgccctg tttaccctct cctcgctcac
      301 ggcacaaagc accctcggca cagggctgcc cttcatcctc gccatgaccc tgatgacctt
      361 cggctttacc attttaggcg cggtcgggct gaaataccgc accttcgcct tcggcgcact
      421 cgccgtcgcc acctacacca cacttaccta cacccccgaa acctactggc tgaccaaccc
      481 cttcatgatt ttatgcggca ccgtactgta cagcaccgcc atcatcctgt tccaaatcat
      541 cctgccccac cgccccgtcc aagaaagcgt cgccaacgcc tacgacgcac tcggtggcta
      601 cctcgaagcc aaagccgact tcttcgaccc cgatgaggca gcctggatag gcaaccgcca
      661 catcgacctc gccatgagca acaccggcgt catcaccgcc ttcaaccaat gccgttccgc
      721 cctgttttac cgccttcgcg gcaaacaccg ccacccgcgc accgccaaaa tgctgcgcta
      781 ctacttcgcc gcccaagaca tacacgaacg tatcagctcc gcccacgtcg actaccaaga
      841 gatgtccgaa aaattcaaaa acaccgacat catcttccgc atccaccgcc tgctcgaaat
      901 gcagggacaa gcctgccgca acaccgccca agccctgcgc gcaagcaaag actacgttta
      961 cagcaaacgc ctcggccgcg ccatcgaagg ctgccgccag tcgctgcgcc tcctttcaga
     1021 cagcaacgac aatcccgaca tccgccacct gcgccgcctt ctcgacaacc tcggcagcgt
     1081 cgaccagcag ttccgccaac tccgacacag cgactccccc gccgaaaacg accgcatggg
     1141 cgacacccgc atcgccgccc tcgaaaccgg cagcctcaaa aacacctggc aggcaatccg
     1201 tccgcagcta aacctcgaat caggcgtatt ccgccatgcc gtccgcctgt ccctcgtcgt
     1261 tgccgccgcc tgcaccatcg tcgaagccct caacctcaac ctcggctact ggatactact
     1321 gaccgccctt ttcgtctgcc aacccaacta caccgccacc aaaagccgcg tccgccagcg
     1381 catcgccggc accgtactcg gcgtaatcgt cggctcgctc gtcccctact tcaccccgtc
     1441 tgtcgaaacc aagctttgga tcgtcatcgt cagtaccacc ctctttttca tgacccgcac
     1501 ctacaaatac agtttctcca ccttcttcat taccattcaa gccctgacca gcctctccct
     1561 cgcaggtttg gacgtatacg ccgccatgcc cgtacgcatc atcgacacca ttatcggcgc
     1621 atcccttgcc tgggcggcgg tcagctacct atggccagac tggaaatacc tcacgctcga
     1681 acgcaccgcc gcccttgccg tatgcagcaa cggtgcctat ctcgaaaaaa tcaccgaacg
     1741 cctcaaaagc ggcgaaaccg gcgacgacgt cgaataccgc gccacccgcc gccgcgccca
     1801 cgaacacacc gccgccctca gcagcaccct ttccgacatg agcagcgaac ccgcaaaatt
     1861 cgccgacagc ctgcaacccg gctttaccct gctcaaaacc ggctacgccc tgaccggcta
     1921 catctccgcc ctcggcgcat accgcagcga aatgcacgaa gaatgcagcc ccgactttac
     1981 cgcacagttc cacctcgccg ccgaacacac cgcccacatc ttccaacacc tgcccgaaac
     2041 cgaacccgac gactttcaga cagcactgga tacactgcgc ggcgaactcg acaccctccg
     2101 cacccacagc agcggaacac aaagccacat cctcctccaa cagctccaac tcatcgcccg
     2161 gcagctcgaa ccctactacc gcgcctaccg acaaattccg cacaggcagc cccaaaacgc
     2221 agcctgaaaa agtttcggca ttttgtaaga gagggcagat tgtcagacag gttgcaagat
     2281 agtggataaa gctttgcgca gggtaaatgc gtagcaactg aaccgtcatt cccacgaacc
     2341 tacatcccgt cattcccacg aaaacagaaa accaaaaaca gaaacctaa
//
LOCUS       DAWKOG010000205         2369 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.205, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000205 DAWKOG010000000
VERSION     DAWKOG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.205"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(119..1555)
                     /locus_tag="WJK66_001980"
     CDS             complement(119..1555)
                     /locus_tag="WJK66_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEZ7579629.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     IGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIENKHGIV
                     AVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKFGIRRIFVVGLSTGSW
                     ARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLRRHPDE
                     KFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATAVHSIP
                     YAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKVVSRML
                     IQMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            complement(1578..2198)
                     /locus_tag="WJK66_001981"
                     /pseudo
     CDS             complement(1578..2198)
                     /locus_tag="WJK66_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
BASE COUNT          738 a          566 c          553 g          512 t
ORIGIN      
        1 ggaataagaa ttcgggacaa aatgtttaaa ccattttgtc ccgactgctg tgatgcggtt
       61 tttttgaact agcaggacgt tacctaaaac ctgcgttgcc tgaaaccctt taagacggtt
      121 aaatccctct gccgtatttg tattctacaa tcatattatc gtcggtaatg acttccgccc
      181 cagccgaagg ttccgtcatc tgaatcagca tacgagagac aaccttttgt gctgcagcat
      241 ccacggtgct gctgtcaaat acgtgcctgc cgctttccgg ccaaatcaac cgggagagac
      301 gttgcttgag cagttcttta ttagggaaaa ctaccggggt tgccgagccg actaccatat
      361 gcccatagcg gtatgcatag ggaatactgt gtacggcggt agcaaaagca tgcgggctgt
      421 gcgtggtatt aaacattaca ataccatccg gggtaaggtg gctttgcacc tgttttaaaa
      481 attccgcact caacaggttg gtggaatagg cacgccagta ccaagtcgta ttcatcaaaa
      541 tcaggtcgaa tttttcatca ggatggcgac gcagccattt cctaccgtca tccaatacaa
      601 tttcaacacg tttgtcctgc aaaagcgggg cgatttgcgg ctcgtccgcg ataaggctac
      661 ggtatgccgg attgatttcc gcaacaatca tcgactgcat ttccggaatg gcagacaaga
      721 cgcgcgccca cgaacctgta ctcaatccaa cgacgaaaat gcggcgtatg ccaaacttca
      781 gggagggtag cagataggca cgttcgatgc cgttgacact attgaataca tcggtattgt
      841 atgcgccgtc gtatacattc gccccataaa caaccttatc accatctcta tggtaaaccg
      901 caacaatgcc gtgtttgttt tcaatcagcc tatccggacg gtcagcaata ttttgaaaga
      961 cagaatccgg cagtaggaac atgaggatgc cgaacattag ggaaactgct accgacactg
     1021 cattcagtcg gagacttttt tggaacagtg tacaaaaaga aatcaaacag atgagcaggt
     1081 aaatctgttg ggtggacaag aaatcaagta tcacaaagcc gataaggacc ggacccaatg
     1141 cactgccggc aacgttggcg aaataaacat tggaaacctg tcgtccggat ttgttgccat
     1201 ccgtacccac atggtgtacg agcgggaaaa tcaaccctct gacgacggca gacagggtaa
     1261 tgaagatacc ggcgtggtgg acgaagccgg aaaaacccgt caacaaccac gcagcaccca
     1321 aaatcaaaaa gtcggcaata cccgcccaca agaagcactg cccgataaag ggaatatcaa
     1381 caaagcggct gcggcaaatc cgtttgccaa aatacgcgcc gatggcgata ccggtcagaa
     1441 aacaggcaag agtaaatgaa aatgcctgag gcacggactg tgctgcgaac gaaaacatcc
     1501 ttacccacaa gacttctata cccaagctca ataagccgct aaggaaagaa agcatataaa
     1561 tcaaactagt attcggttta gtgttcacta tatccatcct ttctgtaacg cagccatact
     1621 gaagcagcaa tcagaaggtt aaagcaggct gtcagcgcaa tggtttggga gagggtaaaa
     1681 aagacgtaga aaaattcggc ggcggcaagc gatccgagtg ccgcacccaa agtgttgaaa
     1741 aaatataagg taccgataga ctcgccaaca ttatgtattt tccggttaaa aaaacaggtc
     1801 agcaagggca aggtcgcgcc catcataaag gtaggaagca gcaataagag gaaattggca
     1861 gcagcgatga tgggcaaatc agcctcaact aaaagatgcc ccaagccgga aatcagaccc
     1921 ctgcttacca aaccgaacag accgatggat acttcagcga tgcaaaacag ggggatgata
     1981 cttgaaggaa aacggtcagc aatgcgtcca ccgaaatacg cacctacacc caagccgacc
     2041 ataaatacag aaataatgac agtaatcgaa cttaaatcga tacctatgtg actgaataga
     2101 agcctctgcc agctgaccta gtaaatcagg gcgcagaatc cagaggcgaa aaacaccagc
     2161 gacaatcctt tcagggtttt acttgcggtc gaattcatat tttctatttt caaataggcg
     2221 gaactataaa tgaaatgtat caatgtgtaa acatttgtat taaccgtagg aaatcaccgt
     2281 attgctcaaa ctgcaggccg ggaaaataaa ctcgtaagcc cgcaaacggc gtatcactgt
     2341 tcaaactgct gtgcggcttc acggtgtta
//
LOCUS       DAWKOG010000206         2338 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.206, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000206 DAWKOG010000000
VERSION     DAWKOG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.206"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            43..2256
                     /locus_tag="WJK66_001982"
     CDS             43..2256
                     /locus_tag="WJK66_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ7579630.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPDNDKKLVLAARSLA
                     EAHYPADAVTPYGEPLSDHFLGAAQMVDELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNVPDNPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILIDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSNKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPRVLASLGDVKGVLSVTRL"
BASE COUNT          599 a          760 c          566 g          413 t
ORIGIN      
        1 ctgttacaat aaactcgttt ttatcggaac gaaagaccca tcatgaccgc catcagcccg
       61 attcaagaca cgcaaagcgc gactctgcaa gaattgcgcg aatggttcga cagctactgc
      121 gccgcgctgc cggacaacga taaaaaactt gtcttagccg cccgttcgct ggcggaagca
      181 cattaccccg ccgatgccgt cacgccgtat ggcgagccgt tgtccgacca cttcctcggc
      241 gcggcacaaa tggtcgacga actcgacctg ctgcccgatg ccgtcgccgc caccctgctt
      301 gccgacatcg gacgctacgt ccccgattgg aatctattgg tttccgaacg ctgcaacagc
      361 accgtcgccg agctggtcaa aggtgtggac gaagtgcaga aactcaccca cttcgcccgg
      421 gtggacagcc tcgccacgcc agaagaacgc gcccagcagg cagaaactat gcggaaaatg
      481 ctgctggcga tggttaccga catccgcgtc gtgttaatca aactggcgat gcgtacgcgc
      541 accctgcaat tcttaagcaa cgttcccgac aatcctgaaa agcgcgccgt cgccaaagaa
      601 accctcgaca tttttgcccc actcgccaac cgcttgggcg tgtggcaact caaatggcag
      661 ctcgaagatt tgggcttccg tcatcaagag ccggaaaaat accgcgaaat cgccctgctt
      721 ttggacgaaa aacgcaccga gcgcctcgaa tacatcgaaa acttcctcaa tatcctgcgt
      781 acggaactca aaaaatacaa tatccacttt gaagtcgccg gccgtccgaa acacatctac
      841 tccatttaca aaaaaatggt gaagaaaaaa ctcagcttcg acggcctctt tgacatccgc
      901 gccgtgcgga ttctgattga taccgtcccc gagtgttaca ccacgctggg catcgtccac
      961 agcctctggc agcccattcc cggcgagttc gacgactaca tcgccaaccc caaaggcaac
     1021 ggctataaaa gtttgcacac cgtcatcgtc ggcccggaag acaaaggcgt ggaagtgcaa
     1081 atccgcacct tcgatatgca ccaattcaac gaattcggtg tcgccgccca ctggcgttac
     1141 aaagagggcg gcaaaggcga ttcagcctac gaacagaaaa tcgcctggtt gcgccaactc
     1201 ttggactggc gcgaaaacat ggcggaaagc ggcaaggaag acctcgccgc cgccttcaaa
     1261 accgagcttt tcaacgacac gatttatgtc ctgaccccgc acggcaaagt cctctccctg
     1321 cccacgggcg cgacccccat cgacttcgcc tacgccctgc acagcagcat cggcgaccgt
     1381 tgccgcggtg cgaaagtcga agggcagatt gtgccgctgt ccaccccgct cgaaaacgga
     1441 cagcgtgtcg aaatcattac cgccaaagaa gggcatcctt ccgtcaactg gctttacgaa
     1501 ggctgggtca aatccaacaa ggcaatcggc aaaatccgcg cctacatccg ccagcaaaac
     1561 gccgacaccg tgcgcgaaga aggccgcgtc caactcgaca aacagcttgc caaactcacg
     1621 cccaaaccca acctgcaaga gcttgccgaa aatctcggct acaaaaagcc agaagacctc
     1681 tacaccgccg tcggacaagg cgaaatttcc aaccgcgcca tccaaaaagc ctgcggcacg
     1741 ctgaacgaac cgccgcccgt acccgtcagc gaaaccacca tcgtcaaaca gtccaaaatc
     1801 aaaaaaggcg gcaaaaacgg cgtgctcatc gacggcgaag acggtctgat gaccacgctt
     1861 gccaaatgct gcaaacccgc gccgcccgac gacattgtcg gcttcgttac ccgcgatcgc
     1921 ggcatttcgg tacaccgcaa aacctgcccc tctttccgac acctcgccga acacgcgccc
     1981 gaaaaagtac tggacgcaag ttgggcggcg ttgcaggaag gacaagtgtt cgccgtcgat
     2041 atcgaaatcc gcgcccaaga ccgctccggg cttttgcgcg acgtatccga cgcgctcgcc
     2101 cgccacaaac tcaacgttac cgccgtgcaa acccagtccc gcgacttgga agccagtatg
     2161 cggttcacgc tcgaagtcaa acaggtcaac gacctcccgc gcgtcctcgc cagcctcggc
     2221 gacgtcaaag gcgtattgag cgttacccgg ctttaaatac aaaaatgccg tctgaaagcc
     2281 gtataacgct tcagacggca ttttgattga cggggtttgc tattttttgt tgcatagt
//
LOCUS       DAWKOG010000207         2313 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.207, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000207 DAWKOG010000000
VERSION     DAWKOG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.207"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            31..510
                     /locus_tag="WJK66_001983"
     CDS             31..510
                     /locus_tag="WJK66_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEZ7579631.1"
                     /translation="MPSETAIADITRTYSAHYLNPNNRNMTLFPDALPCLDKLKAQGY
                     WLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGLCGELGLDPKEA
                     LVVGDTAHDLHMAANAGAAAVGVATGAHSREQLLSAPHLAVLDDLSELPGFLAQHYA"
     gene            complement(586..1554)
                     /gene="ispH"
                     /locus_tag="WJK66_001984"
                     /gene_synonym="lytB"
     CDS             complement(586..1554)
                     /gene="ispH"
                     /locus_tag="WJK66_001984"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEZ7579632.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVQQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     REVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(1583..2080)
                     /locus_tag="WJK66_001985"
     CDS             complement(1583..2080)
                     /locus_tag="WJK66_001985"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HEZ7579633.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFATLGKTGAAM
                     IIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKT
                     QEEKY"
BASE COUNT          536 a          717 c          558 g          502 t
ORIGIN      
        1 ctgcccgaaa tcatccgcga cctgctcgaa atgccgtctg aaaccgccat tgccgacatc
       61 acacgcactt attccgcaca ttacctcaat cccaacaacc gcaatatgac gctctttccc
      121 gatgccctgc cctgtctgga caagctcaaa gcacaaggat actggcttgc cgtcgccacg
      181 ggcaaagggc gggcgggctt ggacaacgcc atcagccaaa ccgccaccgg cggctattgg
      241 ctcgccaccg cctgcgcggg ggaatatccc tccaaaccct cgcccgaaat ggtattcgga
      301 ctctgcggcg aactgggact cgatccgaaa gaagcattgg tcgtcggcga tacggcgcac
      361 gacctgcata tggcggcaaa cgcaggcgcg gcggcagtcg gcgtggccac cggcgcacat
      421 tcgcgcgaac agctccttag cgcaccgcat ctcgccgtat tggacgactt gtccgaactg
      481 cccggttttc ttgcacaaca ttacgcctga ttggtttccg catcagacac tcgaaacaaa
      541 tgccgtctga agcctgttca gacggcattt gtgttgccca aacattcaac gtttgcacaa
      601 atcgggtttg gtttcgccct cgcggcgcag ctctttgggc aggacgaaca caatgctttc
      661 ttccgcaccc tcgccttcgc gtacggtttc gtgcccccat ccgcgtatgg ttgccagtac
      721 ttcccgcacc aacacttcgg gcgcggacgc gcctgccgtt acgccgactt tgtttttgcc
      781 ctcaaaccat gcgcgttgca ggtagcctgc attatccacc atatacgcat cgattccgcg
      841 cgatgccgcc acttcgcgca agcggttgct gttggacgaa ttgggcgaac cgaccacaat
      901 cacgatgtcg cactgttctg ccaactcttt gacggcggtt tgccggttgg tcgtcgcata
      961 gcagatatct tccttgtgcg gattgcggat attggggaaa cgcgcgttca gcgcggcgat
     1021 gatgtctttg gtttcatcga ccgagagcgt ggtttggctg acataggcga gtttgtcggg
     1081 gtttctgact tcgagttttg ccacatctcc gaccgtttcg accaaaagca ttttgcccgg
     1141 cgcaagctgc cccatcgtgc cttcgacctc gacgtgcccc ttatgcccga tcatgatgat
     1201 ttcacagtct tgggcatcca gtcgggcgac ttccttatgc actttcgtca ccagcgggca
     1261 agtcgcatca aacacgcgga aaccgcgctc cgccgcttct tgctgcaccg ccttcgatac
     1321 gccgtgtgcc gaataaacca gtgtcgcgcc cggcggcact tccgccaagt cttcaataaa
     1381 caccgcacct ttttcacgca ggttgtccac gacgaatttg ttgtgaacga cttcgtggcg
     1441 cacataaatc ggcgcgccga actcttccaa agcacgttcg acaatactga ttgcccgatc
     1501 cacaccagcg cagaagccgc gcggattggc aaggatgatg gttttctcgt tcataagccc
     1561 ggtatttcgt tttcagacgg catcaatatt tttcttcttg ggttttacgg tggacgatgt
     1621 tgtccaacac cgccaacacc gcaccgacgc agataaagct gtcggcaata ttaaaggcgg
     1681 gataaaacca attttgccaa taaaacaata agaaatcgac gacatgaccg tgtatcaggc
     1741 gatcgatgac attgcctaac gcaccgccga taatcatcgc cgcacccgtt ttgccgaggg
     1801 ttgcaaactc atcgcgcaag atggcgcgta ccaaatacgc gctcaccgcc accgccagca
     1861 ccaaaaaaaa gtatttttgc cagccgccct gatcggcaag gaagctgaac gccgcacccg
     1921 ggttgtacac caaggtcaaa tcgaaaaagg aaggaatgac attgacgcgt tcccgatact
     1981 gaaacgacga cagcaccgcc cacttcgacc actggtccag cacgatggcg gcaagtgcca
     2041 atacccaata gcgcgtttta cttgaaacag atgaagacat atttttcaac agccggtaaa
     2101 agagtaccat tttacccgaa aacccccttt cctgtacccg aaacggcaaa tgccgtaatc
     2161 ttaaaacccg tcattcccga caacaccgta atctcgaaac ccgtcattcc cgcgcaggcg
     2221 ggaatccaga cctgtccgca cagaaactta tcggataaaa acagttgccc aaaccccgcg
     2281 ttctatagtg gattaaattc aaaccagtac ggc
//
LOCUS       DAWKOG010000208         2307 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.208, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000208 DAWKOG010000000
VERSION     DAWKOG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.208"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(53..1462)
                     /gene="dacB"
                     /locus_tag="WJK66_001986"
     CDS             complement(53..1462)
                     /gene="dacB"
                     /locus_tag="WJK66_001986"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEZ7579634.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAADSTDILTDPPLPHIFAQNNLKITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGISDTPEG
                     AQTLAVAHSKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVANNI
                     ISGGDGWLDAKLMCKERRA"
     gene            1627..2193
                     /locus_tag="WJK66_001987"
     CDS             1627..2193
                     /locus_tag="WJK66_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ7579635.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTVFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          528 a          564 c          663 g          552 t
ORIGIN      
        1 tcctagttat ctaggacagc cccttaaatt taatccacta tattttcctg tttcaggctc
       61 ggcgttcttt gcacatcagt ttcgcatcca gccagccatc gccgccggag atgatgttgt
      121 tggcaacgaa gttgtccaag tcgggcagca gggaaacggc gcggccgctg ttgatgatga
      181 cgaccaccgc catcggtttg tcgcccagcc aataacctgc aagggcgcgg acattgttga
      241 gcgtgccggt ttttaagcgc aacagcccgc cgctttgttt gaagcggttg cgtaaagtcc
      301 cgtctgtgcc ggcgatgggc agcgtatcga tgaaatcttg tgcaaacggg ctgaaataag
      361 ccgtttccaa catttgcgcc atcattctcg ccgttaccct ttcttttctg gacagacctg
      421 aaccgttttc caaaaccaaa tccgcaacat cgatgcccga cacggcaagt tcacgccgga
      481 cggcagacgc tgcctgttcg gaaacggcgg gcagtttgcc gtcgccgccg agtttgagga
      541 agacggaacg cgcaattaga ttgtccgaac gcttgttcat gtccgtcaaa atttccttca
      601 tcggctttga gtgtgcaacg gcaagcgtct gcgcgccttc cggcgtgtcg gatatgccga
      661 tgccgtctga aatccgtccg ccgccgagca gccagtggtt ggtaaaactt tgccggatca
      721 gttcgtcaag cgcgaacatc cggacaccga caggcttgcc caaacagctc tcgggaatat
      781 tgccgcgcaa tttcagcgta ttgtccgaaa aagatgcacg catcagtttt ttgatcgaag
      841 ggcaggcagc ttgggaggcg gtaattttca agttgttttg ggcgaaaata tgcggcaaag
      901 gcggatcggt gaggatgtcg gtactgtcgg cggcattgcg ttcggcgcgc accataacca
      961 taccggcaga cagcatagtt ggattggggg gcgtcataaa cggcgaaccg ctgtcggctt
     1021 cgaaatcgtc ggggctgccg acttcgcccc acaggctgtg gtcgagcatc aggtgtcccg
     1081 tgatattgcg tatgccttgt tcgcgcaact ggcgttggac ggcaagcagg ttttcctgat
     1141 tgaaaacggg gtcgccgctg cccgcccaat acaggtttcc gtcaagcgtg ccgtcgttta
     1201 ccgtaccgtt gcttttaaac tcggtcgccc agcggtaatt gctgccgaag gttttgaagg
     1261 cggcaaacgc ggtaacgagt ttcattgtgg aggcggggtt gacggggaca tccgagcggt
     1321 ggtcaatgat gacttttccg ctgtcaagct cttggacata tacggcgatt tcgttttgcg
     1381 gaatgcgacc tgtatcgagc gcgtgtgcgg cgagggaggc gagaagcagc agcagggagg
     1441 ccgctgtttt ggggaaattc ataggtaaat ctgttacata aaaaaagcaa ttataaggca
     1501 aagccggata agtgggaacg ctgtggcggc gggccggctt gtttgcaggg gaaatcacat
     1561 atataataat cgctatcatt atgaaatgat tgagacgcac aaacttaata ttcaggagga
     1621 atgattatgg ctaagaaaat cagtattttg gtgggcagcc tgcgccgtgc ttcttttgcg
     1681 cgcaaagtgg cattgaatgc ggcggagatg ttccccgaag gctggcaggc ggaaatcgtc
     1741 gaaatcggac atctgccgct ttacaatttc gattatgacg accctgcggt ggaagatgtg
     1801 ccgctgcccg aaagctacac ggttttccgc gaaacgatta aggcttcgga cggcattttg
     1861 ttcgttacgt ccgaaaataa ccgcaccatt cccgcctgtt tgaaaaatgc ggtggacatc
     1921 ggctcgaaac cgaatgccga cgtggcgtgg aaaaacaaac cggccggcat catcagccat
     1981 tccgtcggca agatgggcgg ttacagttcg caaaaaaacc tgcgccttgc gctgtcttat
     2041 ttcgatatgc ccgtaaccgg acagccggaa gtgtttttgg gcaattcgcc gacactgttt
     2101 gatgaaaacg gcaaattgat cgactcggca agggattttg ttcagtcata tattaatcag
     2161 tttgtcggtt tgattgaaag aaatgccaaa taaacaaaca agtcgaaagg ccgcaaaacc
     2221 ggttcgggtt ttgcggcttt tttatatggg ctgggcagcg tcaaggtttg ccgtttgccg
     2281 gataatgcgc ggcaaatgac cggcgtg
//
LOCUS       DAWKOG010000209         2296 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.209, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000209 DAWKOG010000000
VERSION     DAWKOG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.209"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(253..1332)
                     /gene="aroB"
                     /locus_tag="WJK66_001988"
     CDS             complement(253..1332)
                     /gene="aroB"
                     /locus_tag="WJK66_001988"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEZ7579636.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIITNET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMTGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPARELSAGMAEVIKYGTLGDISFFEWLEQHMPELMALERAPLIQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAVAAGCVLAA
                     RLSEQLGKISAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
     gene            complement(1412..1924)
                     /locus_tag="WJK66_001989"
     CDS             complement(1412..1924)
                     /locus_tag="WJK66_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEZ7579637.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
BASE COUNT          478 a          549 c          707 g          562 t
ORIGIN      
        1 tttaagttgc tgttttgggt ttctgttttt tgtggaaata acgagattct agggttgcag
       61 aaacttctcc gaaacaacaa aaacctttcc accgtcattc ctgcgaaagt gggaatctag
      121 aaatgaaaag caacgggaac ttatcggaaa cgaccgaaac cgaacggacc ggattcccgc
      181 ttttgcggag aaagatgatg gaaaatcatc atcgtgcgtc aaacccgcgc ggcagcacat
      241 cggcagagga aatcagagat acggttgcag ggtgcggcgg aggatgtccg tgtcggtaat
      301 ttcggtgatg ttggcttcgc ccagccggtt cagaccgata aagcgcatga tgccgccgct
      361 gactttttta tcgtggctca tgtgttccag ccatttttca aaggcaaaca cgggtggcgc
      421 ggacggcagt ccggcggctt cgagcagggc ggcgagccgc gcggtatctg cggcggagat
      481 tttgcccagt tgttcggaca aacgcgccgc caacacgcag ccggcggcga cggcctcgcc
      541 gtgcagccaa gtaccgtaac ccatctcggt ttcaatggcg tgtccgaagg tgtgtccgag
      601 gttgagccat gcgcgtatgc cctgttcggt ttcgtcttgg gcgacgatgt ctgccttcat
      661 ttggcaacag cggtacacgg cttggatgag gggcgcgcgt tcgagtgcca tcagttcggg
      721 catatgctgt tccagccatt caaaaaaact aatgtcgccg agcgtgccgt atttgatgac
      781 ttccgccata ccggcggaca gttcgcgggc gggcagggtg tgcagcgtgt ccaagtcggc
      841 aagcaccgcc tgcggctggt agaacgcgcc gatcatattt ttgccgagcg ggtggttgat
      901 ggcggttttg ccgccgacgg aggaatcgac ctgacttaat aaagtagtgg gaatttgaac
      961 aaatggcgcg ccgcgttgat aagtagccgc cgcaaaaccc gtcatatcgc cgatcacgcc
     1021 gccgcccagt gcgatgagcg tggttttgcg ttcggcgcgg ttttgcatca gcccgtcaaa
     1081 gatgaggttg agcgtctgcc agtttttgtg cgcctcgccg tcgggcagga tgatgctgaa
     1141 atgggatacg cctgccgcat ccaatgccgt ctgaagcgtg ccgaggtaga gcggggcgac
     1201 ggtttcgttg gtgatgatgg cggcgcgttt gcccaaatgc ggtttgagca ggcttcctgc
     1261 ctgcggcagc agtccgttgc cgataaagat ggggtagctg tgggacgggg tgtgtacggt
     1321 cagtgttttc attgttgttc cttaaagttt gaaccgccgg cccgccgggc ggggcgcggt
     1381 ttggttttct gggcgcggcg catatgccgg tttatcggga taagcgtttg agcagggttt
     1441 gcacggtttc ccggcagttt gccgattcta cggtaaagtc ggcggtttgg cggtaaacgg
     1501 ggtcgcgtgc ggcgtagagt tcacgtaatt tcgccaaagg atcggcaact tgcagcaaag
     1561 gacggctgtt gtcgcagcgc gtgcgttcga gcagggtttc gggcggggcg tgcagataga
     1621 cgaccgtgcc gcttttgcgg ataagggcgc ggttttcttc ttttaacacc gcgccgccgc
     1681 cggtggacag gacgatatgg ggcaggataa ccagcttttt gagtatggcg gtttcgcgcg
     1741 aacggaatcc ctgttcgcct tccatttcaa atatggtggg gatgggaacg ccggcggctg
     1801 cggcgatttc gtgatcgctg tcgtaaaaac ggtaatccag ccgctgcgcc atttgccggc
     1861 ccagcgtggt tttgcccgcg cccatcagtc cgatgaggat gagtttgccg ttaaagtttt
     1921 tcatcacaat tccttaatgt ttgacacccc gcctttcggg gcgacagggt tcgggcttgt
     1981 cgggttacgg cggaatttta tacgaaatcg gcagggcggc ggtacgtttg gaaaaataac
     2041 ccgaccatcc cgaacctttc tgattttaag gacaaataaa agaaatcagg gaggtttttt
     2101 atttcaggct gtgttttgac aatccgttga tttcacttat ttgtcaggaa aaggcaatta
     2161 tctttgctta ggtaaacaat tatccaattg aatatattga agataatatg tttatcaata
     2221 ctatagtgga ttaacaaaaa ccagtacagc gttgcctcgc cttggctcaa agagaacgat
     2281 tctctaaggt gctgaa
//
LOCUS       DAWKOG010000210         2288 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.210, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000210 DAWKOG010000000
VERSION     DAWKOG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.210"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            19..960
                     /locus_tag="WJK66_001990"
     CDS             19..960
                     /locus_tag="WJK66_001990"
                     /inference="COORDINATES: protein motif:HMM:NF016702.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HEZ7579638.1"
                     /translation="MSATEIEKAKAKITAYSKLVAGTASAVVGGDVNTAANAAQIAVE
                     NNTLYPRCVGAKCDEFQKEQQKWIRENPEEYREVLLLQTGFIPIIGDIQSFVQAQTAA
                     DHLFALLGVVPGIGESIQAYKVAKAAKNLQGMKKALDKAATVATAQGYVSKTKIKIGQ
                     IELRVTAATDKQLLKAIGEGRDTTGKMTEQLFDSVAKQNGFRVLSGGKYGGNNGFDHV
                     WQAADGSVVLIVESKQIRNGTVQLNPNGAGGYTQMSEDWIKQVANSLPDGSPAKAAVF
                     KAEREGKLKTAIAGVDRQTGKAVILSVKVPSKTNIRR"
     gene            963..1898
                     /locus_tag="WJK66_001991"
     CDS             963..1898
                     /locus_tag="WJK66_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HEZ7579639.1"
                     /translation="MGHNMMTTQKWYEHITNVIIGNTANFNSGCPEAIDYVDERKGVP
                     LAAMRHILMYTEAAASHAYLFEHDLNKFKQYAYVAGKLGILRSVNSTDPEPFFFPCDM
                     LNIQNPMFLMLMSDSPQLREFLVRNIDNIANDTEAFINRYDLNRHMIYNTLLMVEGKQ
                     LDRLKQRSEKVLAHPTPSKWLQKRLYDYRFFLAFAEQDAEAMKAALEPLFDKKTARMA
                     AKETLSYFDFYLQPQIVTYAKIASMHGFDLGIDHEIAPRDLIVYDPLPADEYQDIFDF
                     MKQYDLSYPYEYLQDWIDYYTFKTDKLVFGNAKRE"
BASE COUNT          684 a          489 c          535 g          580 t
ORIGIN      
        1 aatactgatt tcagtcgtat gagtgcgacc gaaatcgaaa aagctaaagc gaagattact
       61 gcctattcaa aactggttgc cggcactgcg tctgccgttg taggcgggga tgtgaataca
      121 gcggcgaatg cggcacagat agcggtggag aataatactt tgtatcctag atgcgttggt
      181 gcaaagtgtg atgaatttca aaaggaacaa caaaaatgga tacgtgaaaa tcctgaagaa
      241 tatcgagaag ttttgcttct tcagacagga tttattccaa ttatcggtga tatacagagt
      301 tttgtacaag cacagaccgc tgccgatcac ctgtttgctt tgctgggtgt ggttccgggt
      361 atcggtgaat cgatacaggc ctataaagta gcgaaagcgg caaaaaattt acaaggcatg
      421 aaaaaagcct tggacaaggc agcaaccgtt gccactgcac agggctatgt cagcaaaacc
      481 aaaatcaaaa tcggtcaaat tgaattaagg gttactgcag caactgacaa acaattgctg
      541 aaagctattg gcgaaggaag ggacacgaca ggtaaaatga ccgagcagtt atttgactct
      601 gtagctaaac aaaatggctt cagagtgctt tcgggcggca aatacggcgg aaataacggt
      661 tttgatcatg tatggcaggc tgccgatggt agtgtcgttt tgattgtaga aagtaagcag
      721 attaggaacg gtacggtaca gctgaatccg aatggtgcgg gtggatatac gcaaatgagt
      781 gaggattgga ttaaacaagt tgcaaatagc ttacctgatg gtagtcctgc taaagctgct
      841 gtcttcaaag cggagcggga aggcaaatta aaaacggcaa tagcaggcgt tgatcgtcaa
      901 acaggtaagg ccgttattct ttctgtcaaa gttccttcta aaaccaatat aaggagataa
      961 caatggggca caatatgatg accacccaaa aatggtatga gcatattact aatgtaatca
     1021 taggcaatac tgctaatttc aatagcggtt gccctgaagc tatagattat gtagatgaaa
     1081 gaaaaggcgt tccgcttgca gcgatgagac atattttaat gtatactgaa gctgcggctt
     1141 cccatgccta tctatttgaa catgatctta ataaattcaa gcaatatgct tatgttgcag
     1201 gaaagctggg tattttgcgg agtgtaaatt ctacagaccc tgaacccttc ttctttccct
     1261 gtgacatgct caacattcaa aatccgatgt ttctgatgct gatgagcgac agcccacagc
     1321 tgcgcgagtt tctggtgcgc aatatcgaca acatcgccaa cgatacagaa gcctttataa
     1381 accgctacga cctcaaccgt catatgattt acaatactct gctgatggtg gagggtaagc
     1441 agcttgatcg gttgaaacaa cgtagcgaga aagtcttggc gcatcccacc cctagcaaat
     1501 ggctgcaaaa gcggttgtac gattaccgct tcttcctcgc tttcgccgaa caggatgccg
     1561 aggcaatgaa agccgcctta gagccgcttt tcgataaaaa aaccgcgcgt atggctgcca
     1621 aagaaacatt gtcctatttc gatttctacc tgcagccgca aatcgttacc tatgccaaaa
     1681 tcgcatccat gcacggtttc gatttgggca tagaccacga aatcgcaccg agggatttga
     1741 ttgtttacga tccgctgcca gcagacgaat atcaagacat cttcgatttt atgaaacagt
     1801 atgacttgtc ttacccgtat gaatatctgc aggattggat agattactat acgttcaaaa
     1861 ccgataagct ggtatttggt aacgcgaagc gagagtgagc cgtaaaactc tgagctcctg
     1921 ttttatagat tacaacttta ggccgtctta aagctgaaag attttcgaaa gctataaatt
     1981 gaagcccttc catagtacat agatctgtgt tgtggcgagg ctttaccacg ctgattgccg
     2041 gagaagaact caacctgctg gcaaaacaag gcatgagatc tttgcaataa catgagttga
     2101 gacctttgca aaaaagccct tccccgacat ccgaaaccca aacacaggat ttcggctgtt
     2161 ttcgtaccaa atacctccta attttaccca aatatcccct taatcctccc cggatacccg
     2221 ataatcaggc atccgtgctg ccttttaggc ggcgcgggcg cacttagcct gttggcggcc
     2281 ttcaacag
//
LOCUS       DAWKOG010000211         2286 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.211, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000211 DAWKOG010000000
VERSION     DAWKOG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.211"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            20..748
                     /gene="rpe"
                     /locus_tag="WJK66_001992"
     CDS             20..748
                     /gene="rpe"
                     /locus_tag="WJK66_001992"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEZ7579640.1"
                     /translation="MTTYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPYTLEKIRQV
                     RAMLDRYEEKSGRRIAIEVDGGIKTDNIAAAAQAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEMPSEN"
     gene            complement(771..1802)
                     /gene="ruvB"
                     /locus_tag="WJK66_001993"
     CDS             complement(771..1802)
                     /gene="ruvB"
                     /locus_tag="WJK66_001993"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEZ7579641.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQH
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLVGATTRAGMLTNPLRDRFGIVSRLEFYENRDLATIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDVQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(1884..2192)
                     /locus_tag="WJK66_001994"
     CDS             complement(1884..2192)
                     /locus_tag="WJK66_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579642.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQVRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
BASE COUNT          468 a          542 c          730 g          546 t
ORIGIN      
        1 gatattgaag gaaaaaaaca tgactactta ccgtatcgca cccagcattt tatcggcaga
       61 ttttgcacgg cttggcgaag aggtggaaag cgtcattgcg gcaggtgcag atctgatcca
      121 ttttgacgtg atggacaacc attatgtgcc gaacctgacc ttcgggccta tggtttgcgc
      181 ggcgttgaag ccttatgcaa gcgtgccgat cgatgtgcat ctgatggtcg aacccgtgga
      241 tgacctgatt cagtcgtttg ccaaagcagg agcatcaatc atcacgttcc atcccgaagc
      301 gagccgccat attgaccgca gcttgagcct gattcgggac atgggctgtc aggcggggct
      361 ggtgttgaat ccggcaacgc ccgtatatct gttggaaaac gtattggaca ggctggatat
      421 ggttttgctg atgtcggtaa accccgggtt cggcggtcag agcttcattc cgtatacttt
      481 ggaaaaaatc agacaggttc gtgccatgct tgaccgctat gaagagaaaa gcggcagacg
      541 tattgccatt gaagtggacg gcggcatcaa aaccgacaat atcgccgccg ccgcccaagc
      601 cggcgcggat acctttgttg ccggttctgc gattttcggc aaaccagact ataaggcggt
      661 gattgatgcg atgagggcgg aactggaaaa aagcggcagg ctgaaccttg cgccaatgat
      721 tgaaatagaa atgccgtctg aaaattagct ctgtttcaga cggcattgcg ttatttttcg
      781 acgggcagcc cgaaatgcag gtaggcgcgt tcggtcgcca tcctgccgcg cggggtgcgt
      841 tgcaggaagc cttgttggat gagatagggt tcgataacgt cttcgatggt gtctgttgat
      901 tcgccgatgg cagcggcaac attgtccaaa ccgaccgggc cgccgccgaa tttgtgcaaa
      961 acggcttcga ggaatttcct gtccatcacg tccagcccct gcacgtccac gtccagcata
     1021 cttaaagcgg catcggcgat gccgccgtcg attgtgccgt tgtttttcac gtcggcgaaa
     1081 tcgcgcacgc ggcgcaacag gcggttggcg atgcgcggcg taccgcggct gcgtttggcg
     1141 atttcttccg cgccttcttc ggacatatcg agttgcagga gttgtgccga acggctgacg
     1201 atggtggcaa ggtcgcggtt ttcgtaaaac tcgaggcggg agacgatgcc gaagcggtcg
     1261 cgcaacggat tggtcagcat accggcgcgg gtggtcgcgc cgacaagcgt gaagggcggc
     1321 aggtcgattt taacggaacg ggcggcgggc ccttcgccta tcataatgtc gagccggtag
     1381 tcttcaagcg cggggtagag gatttcttcg acaacagggc tgaggcggtg gatttcgtcg
     1441 ataaacaata cgtcgtgcgg atcaaggttg gtcaaaaggg cggcgaggtc gccggcgcgt
     1501 tcgaggacgg ggccgctggt ttggcgcaaa tttacgccca attctttggc gatgatgtgc
     1561 gccagtgtgg ttttacccag cccgggcgga ccgaaaagca agacgtggtc gagtgcttcg
     1621 ccgcgctttt tggcggcttg gatgaaaatg gcgagctgtt ctttggcttt atgctgcccg
     1681 atatagtcgt ccagcgtttt ggggcggagg gcgcgttcga gcagttcttc ctgtgcggag
     1741 gcggtttggg cggcaacaat gcgttgcggt tgagcggcag tcaggttgtc ggtttgcagc
     1801 atagtgttcc ctttgtcggg tatgccgtct gaacggtcgg cggcgtttca gacggcattg
     1861 aaaagataac ggtcaaaagc gttttacccg cgcgtttcgc gccaaacgag atagtcgcgc
     1921 aggggcggca taggcgggtt gatgcagtgt gggcagatgc ctgtggtgtc ttcgtcgtcg
     1981 ctgtcgatgt gataggcgtt ggggtcgaag cggacttgct gcatcaccgc ctgcgccaac
     2041 gcctgcgcct gaacaacgtc gaaattgaat ttttcgagga tttcgttcaa taggcggatt
     2101 tcgccaccgc taaattccgc actgtgtttg aggatgaggc gttgataatc ttcgttgttg
     2161 attttaggca ataaatcggt tttcagggtc atgatttatg tcgggggcgg aatggctgaa
     2221 gcgtgaatta tagcggatat ggcggcggct gttgcaaagc ttggattggg tatcgtcctc
     2281 aaaact
//
LOCUS       DAWKOG010000212         2268 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.212, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000212 DAWKOG010000000
VERSION     DAWKOG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.212"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..574)
                     /locus_tag="WJK66_001995"
     CDS             complement(<1..574)
                     /locus_tag="WJK66_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEZ7579643.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPSGELAAAIDAKWG
                     SFEKFQEAFSACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWNEVAKRF"
     gene            737..2143
                     /gene="dnaB"
                     /locus_tag="WJK66_001996"
     CDS             737..2143
                     /gene="dnaB"
                     /locus_tag="WJK66_001996"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEZ7579644.1"
                     /translation="MNDYAAMPSEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLSKAVVKLSDAPMYIDETPGLTALELRARARRLARQFNGKLGLI
                     VIDYLQLMSGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVEQRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
BASE COUNT          547 a          571 c          617 g          533 t
ORIGIN      
        1 cgaagcgttt ggcgacttcg ttccagttga cgatttccca aaaacctttc aggtagttgg
       61 ggcggctgtt gcggtagtcg atgtagtagg cgtgttccca cacgtcgcag gtcagcagcg
      121 gcgtgttttc agtggtcagc ggagttgctg cgttggaagt ggaaaccaaa tccaatccgc
      181 cggcaggggt tttcaccagc cacgcccaac cggagccgaa cgtaccggca gcgcaagcgg
      241 agaatgcttc ttggaatttc tcgaagctgc cccatttcgc gtcgatggcg gcggccagtt
      301 caccggaagg tttgccttga cctttgggcg tgaaacccag ccagtagaaa gtgtggttcc
      361 aagtttgggc tgcgttgttg aacacgccgc ccgaagattt tttcacaatc tcttccaaag
      421 gcaggttttc aaattcggtg cctttgattt gattgttcag gttggtgatg taggtttgat
      481 ggtgtttgcc gtagtggaat tccaaagtct ctttgctcag atgcggggac aatgcgtcca
      541 gttcataagg cagttgcggc agcttatgtt ccattttgtg ctcctaatat tgttttaaat
      601 gttgtatttt ggcagtgttg ctgcaaatag cttggcagcg cacatatttt acctgttttg
      661 aggaatggaa accaattaaa cctgctttac gctataatag aagattgcaa tttcggcacg
      721 acaaatagga tgtaccatga acgattacgc agccatgccg tctgaagacc gtgaggtcgg
      781 cgcattgtcg cttccgccgc actcaatgga ggcggaacaa tccgttttgg gcgggttgat
      841 gctggaaaat ccggcatggg acaggattgc cgatgtggtt tcgggagagg atttctaccg
      901 gcatgaacac cgcctgattt tccgttccat tgccaaatta atcaatgaga gccgtcccgc
      961 agatgtgatt acggttcagg aagatttgca gcggaacgaa gaattggaag cggcaggcgg
     1021 attcgaatac ctgattacgc tggcgcaaaa caccccgtct gccgccaaca tccgccgcta
     1081 cgccgaaatc gtgcgcgagc gttccattat gcgccaactc gccgaagtgg ggacggaaat
     1141 cgcccgcagc gcctacaatc cgcaaggcag ggacgcgggg cagcttttag acgaggcgga
     1201 aaacaaagta ttccaaatcg ccgaaagcac ggccaaatcc aagcagggct ttttggagat
     1261 gcccgatttg ctgaaagaag tcgtacagcg catcgatatg ctctactcgc gcgacaatcc
     1321 cgatgaagtt accggcgtgc cgacggggtt catcgacctc gacaaaaaaa cctcgggcct
     1381 tcagcccggc gacctgatta tcgttgccgg tcgtccgtct atgggtaaga ccgccttttc
     1441 tatcaatatc gccgaacacg ttgccgtaga aggcaagctg ccggttgccg tcttctcaat
     1501 ggagatgggc ggggcgcaac tggtcatgcg tatgctcggc tcggtcggac gcttggatca
     1561 aagcgttttg aaaaccggca ggctcgaaga cgaacactgg ggtcgtctga gtaaagcagt
     1621 cgtcaagctt tccgatgcac ctatgtacat cgacgagacc ccgggtctga ctgcgctcga
     1681 actgcgcgcc cgtgcacgcc gtcttgcacg tcagttcaac gggaaattgg gattgatcgt
     1741 catcgactac ctgcaactga tgtcgggttc ggggcgttcc gacaaccgtg cttcggagct
     1801 gggagagatt tcacgttcgc ttaaagcgtt ggcgaaagaa ttgcaagtcc ccatcatcgc
     1861 cctgtcgcaa ttgagccgta ccgtcgaaca gcgtacggac aaacgcccca tgatgtccga
     1921 ccttcgcgag tccggcgcaa tcgagcagga tgccgacctg attatgttca tgtaccgcga
     1981 cgaatactac aaccaggact cacccatgaa aggccttgcc gaatgtatca tcggcaaaca
     2041 ccgcaacggt cccgtcggta aaatcttcct cacatggacg ggacaattca ccaaattcga
     2101 caatgctgcc tatattcccg aggaggcaaa gatagaagat taaatggcta tataaaaatt
     2161 tattaggcga aatcaggcaa aatcgtttaa aatcatgctg agagattgcc ctaaaaaata
     2221 aaacgcggtc ttgaggcatt tttgcattca gcccgcatat aattgaaa
//
LOCUS       DAWKOG010000213         2175 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.213, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000213 DAWKOG010000000
VERSION     DAWKOG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.213"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            104..1915
                     /gene="typA"
                     /locus_tag="WJK66_001997"
     CDS             104..1915
                     /gene="typA"
                     /locus_tag="WJK66_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEZ7579645.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESSDMRPLFDTILKHTPAPSGSADEPLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHEQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
     gene            1949..2137
                     /locus_tag="WJK66_001998"
     CDS             1949..2137
                     /locus_tag="WJK66_001998"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579646.1"
                     /translation="MPSEKSSDGIFYSDGLCGFLKLFQTAFFLPNQETAAIFPAAYRP
                     VAFRYRVRLPGSMSNNAV"
BASE COUNT          549 a          628 c          568 g          430 t
ORIGIN      
        1 gaaaggcaag gacttcagac ggcatcttca tttcccaaat actgtccggt aaagcgtggt
       61 ataatgcgca gttatctctt acagtttttg aaaaacatta attatgaaac aaatccgcaa
      121 catcgccatt atcgcccacg tcgaccacgg caaaaccaca ttagtcgacc aactgctgcg
      181 ccaatccggc acattccgcg ccaaccagca ggttgacgag cgcgtgatgg acagcaacga
      241 ccttgaaaaa gaacgcggca tcaccatcct cgccaaaaac accgccatcg attacgaagg
      301 ctaccacatc aatatcgtcg acacgccggg acacgccgac tttggcggcg aagtggagcg
      361 cgttttgggg atggtggact gcgtcgtctt gttggtggat gcgcaagaag gtccgatgcc
      421 gcaaacccgt ttcgtgacca aaaaagcctt ggctttgggg ctgaaaccga ttgtcgtcat
      481 caacaaaatc gataaaccgt ccgcccgtcc gagctgggtt atcgaccaaa ctttcgagct
      541 gttcgacaac ttgggcgcga ccgacgagca gttggatttc ccgattgttt acgcttcagg
      601 tttgagcggc tttgccaagc tggaagaaac cgacgagagc agcgatatgc gcccgctgtt
      661 cgacaccatc ttaaaacaca cgcccgcacc gagcggcagc gcggacgagc cgctgcaact
      721 gcaaatttcc caactcgact acgacaacta caccggccgc ctcggcatcg gtcgtatctt
      781 gaacggacgc atcaaacccg gccaaaccgt tgccgtcatg aaccacgagc agcaaatcgc
      841 ccaaggccgc atcaaccagc ttttgggttt caaaggcttg gaacgcgtac cgcttgaaga
      901 agccgaagcc ggcgacatcg tgattatttc cggtatcgaa gacatcggca tcggcgtaac
      961 catcaccgac aaagacaacc ccaaaggcct gccgatgttg agcgtggacg aaccgacgct
     1021 gacgatggac tttatggtca acaccagccc gctggcgggt acggaaggca aattcgtaac
     1081 cagccgccaa atccgcgacc gcctgcaaaa agaattgctg accaacgtcg ccctgcgcgt
     1141 ggaagacacc gccgatgccg acgtgttccg cgtatccgga cgcggcgagc tgcacctgac
     1201 cattttgttg gaaaatatgc gccgcgaagg ctacgaactc gccgtcggca aaccgcgcgt
     1261 cgtgtaccgc gacatcgacg gtcaaaaatg cgaaccttat gaaaacctga ccgtggacgt
     1321 acccgacgac aaccaaggcg cggtaatgga agaactcggc cgccgccgtg gcgaactgac
     1381 taatatggaa agcgacggca acggacgcac ccgcctcgaa taccatattc cagcgcgcgg
     1441 cttgatcggt ttccaaggcg aatttatgac cctgacgcgc ggggtcgggc tgatgagcca
     1501 cgtgttcgac gattacgcgc ccgtcaaacc cgatatgccc ggccgccaca acggcgtgct
     1561 ggtgtcccaa gagcagggcg aggcagtcgc ttacgccttg tggaatctgg aagaccgcgg
     1621 ccgtatgttc gtatcgccca acgacaaaat ctacgaaggt atgattatcg gcatccacag
     1681 tcgcgacaac gatttggtgg tcaacccgct caaaggcaaa aaacttacca acatccgtgc
     1741 cagcggtacc gacgaagccg ttcgcctgac cacgccgatt aagctgacgc tggaaggtgc
     1801 ggtcgagttt atcgacgatg atgagctggt agaaatcacg ccgcaatcca tccgcctgcg
     1861 caagcgttac ctgagcgaat tggaacgccg ccgccatttc aaaaagctag attgatgttt
     1921 actggattta atgtttaaat gatactcgat gccgtctgaa aaaagttcag acggcatttt
     1981 ttattcggac gggctttgcg gcttcttgaa gctgtttcag acggcgtttt tcctacccaa
     2041 tcaagaaact gccgccattt ttccagcggc atatcgcccc gtcgcgtttc ggtatcgggt
     2101 tcggcttccc ggcagcatgt cgaacaatgc cgtttgaagg aatacgcctt ttgagtcctt
     2161 acaagcttag gaaga
//
LOCUS       DAWKOG010000214         2167 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.214, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000214 DAWKOG010000000
VERSION     DAWKOG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.214"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..814)
                     /gene="cysW"
                     /locus_tag="WJK66_001999"
     CDS             complement(<1..814)
                     /gene="cysW"
                     /locus_tag="WJK66_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEZ7579647.1"
                     /translation="MKPYSANPNLTEPRRLRVLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGWLEAQGIQIIFAIPGIILATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIIT"
     gene            complement(1192..2028)
                     /gene="cysT"
                     /locus_tag="WJK66_002000"
     CDS             complement(1192..2028)
                     /gene="cysT"
                     /locus_tag="WJK66_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEZ7579648.1"
                     /translation="MLPLKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     GGFWNTIAEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
BASE COUNT          567 a          606 c          587 g          407 t
ORIGIN      
        1 tggtgatgat gttctgcacc gccagcgtcg ccagtgccaa aagtgccaat acgccggaga
       61 gggcgaatgc gccggtgaag ttgtattcgt tgtagaagat ttcgaccaaa agcgggacgg
      121 tgttggtttc gccgcgtatg tgtcccgata ccacgctgac cgcgccgaac tcgcccatcg
      181 cgcgggcgtt ggtgaggatg atgccgtaga gtaacgccca tttgatgttg ggcagggtaa
      241 cgcgccaaaa catctgccag ccgcttgcgc cgagtatcaa tgccgcctgt tcttcgctgt
      301 cgccctgagc ctgcatcagc gggatgattt cgcgcgcgac aaaggggaag gtaacgaaca
      361 gcgtcgccag aataatgccg gggatggcga agataatctg tatgccttgc gcttcgagcc
      421 agccacccaa tgccgtgtgc gcgccgaaca ataagacgaa cattaaaccg gccaccacgg
      481 gcgatacgga aaacggcaaa tcgagcaggg tagtcagcaa ctgcttgcct cggaagtcaa
      541 aacgggtcag cagccacgcc atcgccacac ccaatacagc attgacggga acgacaatca
      601 gcgcggtaat cagcgtcaat ttgatggcag accacgcttc gggatcgttt aaggatttta
      661 ggtacaaatc ccaaccgcct tttaaggctt cgtaaaacac ggcgacgagc ggcacgacca
      721 gcatcagcag cagaaagccc agcgcggcgg caatcagcaa cacgcgcagc cggcgcggtt
      781 cggtcaggtt gggattggcg gaatagggtt tcatggcggt ggtgttctct ggttggttta
      841 aaatgtttgg aggtcgtctg aaaagggatt gaggggtttc agacgacctt tttgttcatt
      901 aagtagtagg tcggatactt gtatccgaca aggttgctga atcgcaaatg tttgtcggat
      961 acaagtatcc gacctacgaa ttctgtgttt tggggagcgg attcgttttc agacgacctt
     1021 ttatctttta tgctaaaaga gaaataaaaa agtctagctg ccgtcattcc cgcgcaggcg
     1081 ggaatccacc acagggcaac aggaaaacag aaaataaata aggcagctga aattcaccaa
     1141 tggattcccg cctgcgtggg aatgacggta acaggtattt cagacgccgc ctcaaccctt
     1201 cgcgcccgaa cgcctgccca acgcccactg catcacgttc agcgcaaaca gaatcacaaa
     1261 cgaaaccagc agcataaaca acgccaccgc cgacgcgccc tgcacgtcga actgttccag
     1321 cttgcccgta ataatcagcg gtaggatttc ggaaaccatc ggaatgttgc ccgcgataaa
     1381 aatcaccgag ccgtattccc ccgttgcccg cgcgaacatc attcccgcgc cggtcaagag
     1441 tgccggcgtg atttccggca gcagcacgcg gcggaacgtc gtaaagcggt ttgcgcccaa
     1501 agtcgccgcc gcttcctcat attcgcccga taattcctcc aacaccggct gcacggcgcg
     1561 gacgataaag ggcaggctga cgacgaccag cgcaatccaa atgccgacgg gcgtaaacgc
     1621 gattttgatg cccaaaggct cgaaaaaacg gcctatccaa ccgttgggcg catacagggt
     1681 tgccaacgcg atacccgtaa ccgccgtcgg cagcgcaaac ggcaaatcga ccagcgcgtt
     1741 cgccagaccc ttgcccggga attcataacg caccaatacc cacgccacca gcgtgccgaa
     1801 cacgacattg gtcagcatcg cataaaacga catccgcaaa ctcagccaca ccgccgccaa
     1861 cacgttcggc tcggcaatcg tgttccaaaa gccgccccag ccgatttccg ccgccttcgc
     1921 cgccatcatc gcaaacggca agaccacaag cagcgacagg cacaatacgg tcagaccaag
     1981 gctgagtttg aagccgggca gtacgccggg cgttttgagc ggtaacataa aacaatgctg
     2041 aaaataagga aaaggaagga ctactttaac gatgccgtcc gaaaaacgga aagaatggaa
     2101 agtttggtgc aaagacgaat ttgttataaa gcggttggca gtttctcaag cgggcgcgat
     2161 gttttaa
//
LOCUS       DAWKOG010000215         2141 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.215, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000215 DAWKOG010000000
VERSION     DAWKOG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.215"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            30..463
                     /locus_tag="WJK66_002001"
                     /pseudo
     CDS             30..463
                     /locus_tag="WJK66_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            559..1182
                     /gene="rsmG"
                     /locus_tag="WJK66_002002"
     CDS             559..1182
                     /gene="rsmG"
                     /locus_tag="WJK66_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEZ7579649.1"
                     /translation="MERKERLRAGIAALGLDISAAAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMVVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTAHLLKDG
                     GYWAAMKGVYPQGEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            1281..2054
                     /locus_tag="WJK66_002003"
     CDS             1281..2054
                     /locus_tag="WJK66_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEZ7579650.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKASLQSGVYQVLLGDADVKSAAVRSKEGGYGVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRNI
                     RLAEAPSHGMPVMAYDAQAKGAKAYLALADELMARVSGK"
BASE COUNT          420 a          489 c          738 g          494 t
ORIGIN      
        1 gcggtctgct tcggaagagg gggacggcga tgtttgtgaa cgagaaagat ccttatgcgg
       61 ctctgtttgc gggactggtg tttttgacgc tgccgtttgc gttggcggtg catgatgcct
      121 ttgcgcttgc gttcggacgg gcggggctgc tggtgtcggt gtcggacggc ggattcggct
      181 ggcgtggcgg ttgggacggc actgtttggt ttgtgttcgg tgtgtttgcg tttttgaatg
      241 tggttgtgtc ggcgggcctg acgaaactgg cgtacaaaaa gatgatgcgg cggcattcgc
      301 gtggattttc gagctgctgt ttggcagtgc cgccttgggc gggctgcgtg gggaggcggt
      361 gttggaatat gcgtttgccg tgtggctggt gtcgatgctg acactgccca aacgcttgac
      421 gcgcgcgccg gtgcagccgg tggtgtttca caggaaaaaa taggacggaa cgggggatgc
      481 cgtctgaaac ccgacacgcg gtttcagacg gcatgttttt ccgttaacat tacgcctgaa
      541 catggacagg atagggatat ggaacgcaaa gaacgcctgc gtgcaggcat cgccgcattg
      601 ggtttggata tttccgcagc ggcgcaggac aggcttttgg cctatgtgga tttgttgaaa
      661 aagtggaaca aaacctacaa tctgaccgcc ctgcgcgacg aggaaaaaat ggttgtccat
      721 catcttttgg acagcctgac gctgctgccc catatcgagg gtgtgcaaac gatgctggat
      781 gtcggttcgg gcggcggtca gcccggcatt ccggcggcgg tgtgccgtcc ggatgtgcaa
      841 ataacccttt tggatgcgaa tacgaagaaa acggcttttt tacagcaggc ggttatcgag
      901 ttggggttgg acaatgtgcg cgtggtatcc ggacgcgtgg aggcggtttc ggacgtgcgt
      961 gccgacgtgg ttaccagccg tgcgtttgca gaactggcgg actttgtatc gtggacggcg
     1021 catctgttga aagacggcgg ctactgggcg gcgatgaagg gcgtatatcc gcagggggaa
     1081 atcggccgcc tgccgcagga tgtgtgcgtt gaaaaagtcc aaaggctcga cgtgccaggc
     1141 ttggatgcgg aacgccatat cgtcatcctg agcaagcgtt gagcgcgctt cagacggcat
     1201 gaataccttt tttgtgcgga taaaggtaaa attccgcact gtttttcttt tttcaacatc
     1261 agacgggaca cgggcgggac atgagtgcga acatccttgc catcgccaat cagaagggcg
     1321 gtgtgggcaa aacgacgacg acggtaaatt tggcggcttc gctggcatcg cgcggcaaac
     1381 gcgtgctggt ggtcgatttg gatccgcagg gcaatgcgac gacgggcagc ggcatcgaca
     1441 aggcgagttt gcagtccggc gtttatcagg tcttattggg cgatgcggac gtgaaatcgg
     1501 cggcggtacg cagcaaagag ggcggatacg gcgtgttggg tgcgaaccgc gcgctggccg
     1561 gcgcggaaat cgagctggtg caggaaatcg cccgggaagt gcgtttgaaa aacgcgctca
     1621 aggcagtggc ggaagattac gactttatcc tgatcgactg cccgccttcg ctgacgctgt
     1681 tgacgcttaa cggcttggtg gcggcaggcg gcgtgattgt gccgatgttg tgcgaatatt
     1741 acgcgctgga agggatttcc gatttgattg cgaccgtgcg caaaatccgt caggcggtca
     1801 atcccgattt ggatatcacg ggcatcgtgc gtacgatgta cgacagccgc agcaggctag
     1861 ttgccgaagt cagcgaacag ttgcgcagcc atttcgggga tttgctgttt gaaaccgtca
     1921 tcccgcgcaa tatccgcctt gcggaagcgc cgagccacgg tatgccggtg atggcttatg
     1981 atgcgcaggc aaagggtgcc aaggcgtatc ttgccttggc ggacgagctg atggcgaggg
     2041 tgtcggggaa ataggtcaat ccaaatcggg ctgcccgtgc ctttatgctg tttggccggg
     2101 tgcgttaata tggcggatta aaataaaaat acttatatcg t
//
LOCUS       DAWKOG010000216         2048 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.216, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000216 DAWKOG010000000
VERSION     DAWKOG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.216"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            185..1963
                     /locus_tag="WJK66_002004"
     CDS             185..1963
                     /locus_tag="WJK66_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HEZ7579651.1"
                     /translation="MNKIYRIIWNSALNAWVAVSELTRNHTKRASATVKTAVLATLLF
                     ATVQANATDEDEEEELESVQRSVVGSIQASMEGSGELETISLSMTNDSKEFVDPYIVV
                     TLKAGDNLKIKQNTNENTNASSFTYSLKKDLTGLINVETEKLSFGANGKKVNIISDTK
                     GLNFAKETAGTNGDTTVHLNGIGSTLTDTLAGSSASHVDAGNQSTHYTRAASIKDVLN
                     AGWNIKGVKTGSTTGQSENVDFVRTYDTVEFLSADTKTTTVNVESKDNGKRTEVKIGA
                     KTSVIKEKDGKLVTGKGKGENGSSTDEGEGLVTAKEVIDAVNKAGWRMKTTTANGQTG
                     QADKFETVTSGTNVTFASGKGTTATVSKDDQGNITVMYDVNVGDALNVNQLQNSGWNL
                     DSKAVAGSSGKVISGNVSPSKGKMDETVNINAGNNIEISRNGKNIDIATSMAPQFSSV
                     SLGAGADAPTLSVDDEGALNVGSKDANKPVRITNVAPGVKEGDVTNVAQLKGVAQNLN
                     NRIDNVDGNARAGIAQAIATAGLVQAYLPGKSMMAIGGGTYRGEAGYAIGYSSISDGG
                     NWIIKGTASGNSRGHFGASASVGYQW"
BASE COUNT          616 a          484 c          523 g          425 t
ORIGIN      
        1 cttaccggat aaaacagttt ccttagattc cacgtcccag attcccgcct tcgcgggaat
       61 gacgagattt taagttgggg gaatttatca gaaaaccccc aacccccaaa aaccgggcgg
      121 atgccgcacc atccgccccc aaaccccgat ttaaccattc aaacaaacca aaagaaaaaa
      181 caaaatgaac aaaatatacc gcatcatttg gaatagtgcc ctcaatgcct gggtcgccgt
      241 atccgagctc acacgcaacc acaccaaacg cgcctccgca accgtgaaga ccgccgtatt
      301 ggcgacactg ttgtttgcaa cggttcaggc gaatgctacc gatgaagatg aagaagaaga
      361 gttagaatcc gtacaacgct ctgtcgtagg gagcattcaa gccagtatgg aaggcagcgg
      421 cgaattggaa acgatatcat tatcaatgac taacgacagc aaggaatttg tagacccata
      481 catagtagtt accctcaaag ccggcgacaa cctgaaaatc aaacaaaaca ccaatgaaaa
      541 caccaatgcc agtagcttca cctactcgct gaaaaaagac ctcacaggcc tgatcaatgt
      601 tgaaactgaa aaattatcgt ttggcgcaaa cggcaagaaa gtcaacatca taagcgacac
      661 caaaggcttg aatttcgcga aagaaacggc tgggacgaac ggcgacacca cggttcatct
      721 gaacggtatc ggttcgactt tgaccgatac gcttgcgggt tcttctgctt ctcacgttga
      781 tgcgggtaac caaagtacac attacactcg tgcagcaagt attaaggatg tgttgaatgc
      841 gggttggaat attaagggtg ttaaaactgg ctcaacaact ggtcaatcag aaaatgtcga
      901 tttcgtccgc acttacgaca cagtcgagtt cttgagcgca gatacgaaaa caacgactgt
      961 taatgtggaa agcaaagaca acggcaagag aaccgaagtt aaaatcggtg cgaagacttc
     1021 tgttattaaa gaaaaagacg gtaagttggt tactggtaaa ggcaaaggcg agaatggttc
     1081 ttctacagac gaaggcgaag gcttagtgac tgcaaaagaa gtgattgatg cagtaaacaa
     1141 ggctggttgg agaatgaaaa caacaaccgc taatggtcaa acaggtcaag ctgacaagtt
     1201 tgaaaccgtt acatcaggca caaatgtaac ctttgctagt ggtaaaggta caactgcgac
     1261 tgtaagtaaa gatgatcaag gcaacatcac tgttatgtat gatgtaaatg tcggcgatgc
     1321 cctaaacgtc aatcagctgc aaaacagcgg ttggaatttg gattccaaag cggttgcagg
     1381 ttcttcgggc aaagtcatca gcggcaatgt ttcgccgagc aagggaaaga tggatgaaac
     1441 cgtcaacatt aatgccggca acaacatcga gattagccgc aacggtaaaa atatcgacat
     1501 cgccacttcg atggcgccgc agttttccag cgtttcgctc ggcgcggggg cagatgcgcc
     1561 cactttaagc gtggatgacg agggcgcgtt gaatgtcggc agcaaggatg ccaacaaacc
     1621 cgtccgcatt accaatgtcg ccccgggcgt taaagagggg gatgttacaa acgtcgcaca
     1681 acttaaaggc gtggcgcaaa acttgaacaa ccgcatcgac aatgtggacg gcaacgcgcg
     1741 tgcgggcatc gcccaagcga ttgcaaccgc aggtctggtt caggcgtatc tgcccggcaa
     1801 gagtatgatg gcgatcggcg gcggcactta tcgcggcgaa gccggttacg ccatcggcta
     1861 ctccagtatt tccgacggcg gaaattggat tatcaaaggc acggcttccg gcaattcgcg
     1921 cggccatttc ggtgcttccg catctgtcgg ttatcagtgg taagggcttt ttcgcctgtc
     1981 tgctgttggg acaggcggaa ggtttgaagg gaagggtggc gatttgccgc ctgagacctt
     2041 tgcaaaat
//
LOCUS       DAWKOG010000217         1946 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.217, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000217 DAWKOG010000000
VERSION     DAWKOG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.217"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            244..1701
                     /locus_tag="WJK66_002005"
     CDS             244..1701
                     /locus_tag="WJK66_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ7579652.1"
                     /translation="MSRHPARTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGIALIAALATAIKTGLVNLDNFSGILLSTVILAVIVYFTRLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISAGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT          431 a          583 c          448 g          484 t
ORIGIN      
        1 ccgccctatc ggataagtcg cttgactgaa aaggctttac ccgcgccgta tcggaacacg
       61 tcctctaaaa tacaatccgt tgaattgaaa caaatataaa aacattcgac cgcaaaaacg
      121 gcagcgcatc gtttgacaaa gattgaaaat atcggttaaa aaccgatttt catacaaaaa
      181 acaccgctgc cgtccgcatc cgtttcagac ggtattgaga gaaaatcttt taggagaacc
      241 tttatgtccc ggcatcccgc ccgcaccgga gaaaaaacat tcttcggcca ccccttccag
      301 ctttccaccc tcttccatat cgaattgtgg gaacgttttt cattttacgg aatgcagggc
      361 atcctgctga tttacctcta ctacaccgcc gacaaaggcg gcttgggcat agacaaaacc
      421 ctcgccggcg gcattgtcgg cgcatacagc ggcagcgtgt acctgtccac cattttgggc
      481 gcgtggtttg ccgaccgcgt atggggtgcg gaaaagaccc tcttcctctc gggcatcgtc
      541 gtgatgctcg gacacatcgt ccttgccgcc gccccgggcc tgtacggcct tttaatcggg
      601 ctgatattca tcgcattggg cagcggcggt gtgaaatcta cggccagttc tatggtgggc
      661 gcattatacg aacaggacga aatgcgcccg ctgcgcgatg cgggattttc cattttctac
      721 atcgccatca atatcggcgg cttcctaggc ccgctgctga ccggcctgct gcaggaaaac
      781 atcggtttcc attatggttt cggcgcggcg gcggtcggta tggcattcgg cttgtggcgt
      841 tattccttgg gacgtaaaaa cctgccccac cccaccgtcc cccatccgct ttcaaaagga
      901 cagggcaaaa ctgcggccgc cgtcggcatc gccctcatcg ccgcacttgc aaccgccatc
      961 aaaaccgggc ttgtcaacct cgacaatttc tccggcatcc tcctatctac cgtcatcctt
     1021 gccgtcatcg tctattttac ccggctgctg accaaccccc gcgtcagttc cgacaacaag
     1081 cggcacatca tcgcctacat cccgcttttc ctgaccatct gtatgttttg ggcggtctgg
     1141 tttcagattt acaccgtggc aaccgtctat ttcgacgaaa ccgtcaaccg caccatcggt
     1201 tcgtttaccg tacccgtcgc ttggaaagat tctatgcaaa gcctgtgggt catcctgttt
     1261 tccggactta tggcggcaat gtggacaaaa atggggcgca aacagcccaa aaccccgctg
     1321 aaattcgcta tggcggtatt tgttaccggc gcgtcgtttt tgggattcgt cccctttatt
     1381 tccgccggca cgccgatgcc tattgcggtt ttcgcactga ttgtccttgc catcacgata
     1441 ggcgaactga tgatttcccc gattgcgctg tccatctcca ccaaaatcgc accgccttta
     1501 ttcaaaaccc aaatggtcgc ccttaatttc cttgcctttt cattaggctt cactttgggc
     1561 ggcgtattgt ttgaaaaagg ctatcaggcg ggcgacgaaa tcggcttcta tcggctgctg
     1621 ttctacatcg gcgcagccac aggcttcctg ctgctcctgc tcgtccccaa attgaacaaa
     1681 atgctcgaag gcacagacta agtcccgccc cgatgccgtc taaacccttc agacggcatt
     1741 tttccgcata atgaaaccaa accgtttcca cccgacagga caggctcccc ccccaaccgg
     1801 aaggcagcct gccgattgtc atttaaatat ctgactgatt ccgtctgttt gcaagggaaa
     1861 tagccgttta tttccgcttg ggcggaaacc ggttctattg agtaaaaggc attttatccg
     1921 actaaattag tgctgcatca acggaa
//
LOCUS       DAWKOG010000218         1935 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.218, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000218 DAWKOG010000000
VERSION     DAWKOG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.218"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            53..1222
                     /gene="metZ"
                     /locus_tag="WJK66_002006"
     CDS             53..1222
                     /gene="metZ"
                     /locus_tag="WJK66_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEZ7579653.1"
                     /translation="MSKKLHPQTLAIRGGKEQTGYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALESAERAVATSTGMSAIQAAFFTFLQAGDH
                     VISSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGAILVVDNSLLSPIGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQAAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAALIRKQQNGGGIVIGFEVADQEAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            1308..1514
                     /locus_tag="WJK66_002007"
     CDS             1308..1514
                     /locus_tag="WJK66_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HEZ7579654.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
     gene            complement(1613..1725)
                     /locus_tag="WJK66_002008"
                     /pseudo
     CDS             complement(1613..1725)
                     /locus_tag="WJK66_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT          471 a          506 c          500 g          458 t
ORIGIN      
        1 aaacaacggg cgcacgctcc gttgccgaac ggataaaacc gaggaatcac cgatgagcaa
       61 aaaactccat ccgcaaacgc tcgccatacg cggcggcaaa gaacaaaccg gataccgcga
      121 acacaatcag gcattgttcc tgaccagcag tttcatgtgg gacaacgccc aacacgctgc
      181 cgatttgttt tcaaaaaaaa tcaaaggctt cacttatacc cgtaccgcca acccgaccac
      241 cgccgccttt gaaaaacgca tcgccgcttt ggaaagtgcg gaacgcgcgg tcgccacttc
      301 gaccggcatg tccgcgattc aggcggcgtt ttttaccttt ttgcaggcgg gcgaccatgt
      361 gatttccagc cgcagcctgt tcggcacgac cgtcggcttt atcaataaca tcgttaccaa
      421 attcggcatc gaagtgagcc acgtgtcgcc gactgatata aacgaatgga aagccgccgt
      481 caaagccaac accaaactgc tgtttttgga aacgccgtcc aatcccttgg gagaagtggc
      541 cgacttggaa gccttggcgg aattggcgca cggcatcggc gcgattttgg tggtggacaa
      601 cagcctgctg tcgcccatcg gctctcagcc tttgaagcac ggcgcagata tttccgtttc
      661 ttccgcaacc aaagccatag acggacacgg acgcgtgatg ggcggcgtgt tggcgggttc
      721 ggaagaactg atggcgcagg ccgccgttta ctgcaactct tgcgggctgg cgatgtcgcc
      781 gtttaacgcg tggcagttgt tgagcggcgt ggaaaccctg tcgctgcgta tggaaaaaca
      841 gttcgacaac gccctgaaaa tcgcgcaatg gctgcaagcg cagccgcagg ttcaagccgt
      901 gtattacacc ggattgcccg accatccgca ggcggcgctt atccgcaaac agcaaaacgg
      961 cggtggcatc gtcatcggct ttgaagtagc cgaccaagaa gccgcgtgga aagttgtgga
     1021 cggcgtggaa ctcttttccc gaaccgccaa cctcggcgac gtgcgctcga ccatcaccca
     1081 tccgtggaca accacgcacg gcaggatgca gcccgaagaa aaactcgccg ccaacatccg
     1141 cccgggactg gtgcgcctgt cggtcggttt ggaatatgtt ggcgatttga ttgacgattt
     1201 gaaacaggca ctcgcacgct gattgcgtgt ttcagacggc attggacagc agatgccgtc
     1261 tgaaaccctt atcgcgtatg gcatacacat caacaatagg aaaaaatatg gatgaagcag
     1321 tttttgccga ttgggcgttg aaaatctgcc tgaccggctt gattattttt ttgggtttta
     1381 tcgtttggaa tttgggcaag gagtccaaag cgggcaaatt cggcatcgcc attttgtttt
     1441 tggttttggg cttgggcgtg ttcggttttg tgttcaaaga gttattgatt aagtttttgg
     1501 tattgccgaa ataggtttgc ggcctgtcaa aatgccgtct gaaatcataa aaagtaatct
     1561 gcaccctaaa agtttgattt aaccgccaac tgattaaggt gcggattttt atgacaaaat
     1621 atagtggatt aacaaaaacc agtacggcgt tgcctcgcct tgccgtacta tttgtactgt
     1681 ctgcggcttc gtcgccttgt cttgatttaa atttaatcca ctataaaaat catctttccg
     1741 cagtcattcc cacggacgcg aaagtctgtc tttttgaatc ccggataatt ttacatcgtc
     1801 attcccgcgc aggcgggaat ccaaatcatt gggtagcggc aatgttcaaa agtcgtctga
     1861 aaaatcagaa gttctagatt cccgttttca cgggaatgac ggaatttcag acggcatcct
     1921 cccgccccgt cattc
//
LOCUS       DAWKOG010000219         1840 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.219, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000219 DAWKOG010000000
VERSION     DAWKOG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.219"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..251
                     /locus_tag="WJK66_002009"
     CDS             <1..251
                     /locus_tag="WJK66_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579655.1"
                     /translation="LIEVRKKYPYPMDIPRDIVIGISTIIDFLMVPNWELFEIKASPW
                     LPDSVGIHERYERFTTMLRYIFTEKDIVNVRFDYYNKK"
     gene            315..1448
                     /locus_tag="WJK66_002010"
     CDS             315..1448
                     /locus_tag="WJK66_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579656.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEVPE
                     NPNAFVAKLARLFRNADRAVVIVKESMRTEESLAGAVDDGPLQSEKDYLALAVRLSRL
                     KEKAKWFHVTEQEHGEEVWLDYHIGEGGLVAVSLLQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTVHGENYETTGEYRVVWQPDGSVFDKSGGGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDYVDYQNMRELMPRGMKANSLVVGYDADGLPQK
                     IYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADIQTYHTQQTWFLDGGRI
                     VREEKQGDRLPDFPLNLEDLEKEVSRYAEAAARRSGGRRDLSH"
     gene            complement(1535..1810)
                     /locus_tag="WJK66_002011"
     CDS             complement(1535..1810)
                     /locus_tag="WJK66_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579657.1"
                     /translation="MNESEVVLTPEQIQTLYGYAFRGDTYGGWRYLANLGDRYADNAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKIRLMCISVFWIVVFNL"
BASE COUNT          520 a          357 c          465 g          498 t
ORIGIN      
        1 atttaatcga ggttagaaaa aaatatcctt atccgatgga tataccaagg gatattgtga
       61 ttggaatcag taccattatt gattttttaa tggttccaaa ttgggagctt ttcgaaatta
      121 aagcttcccc ttggctacct gatagcgtgg gaattcatga acgttatgaa agattcacaa
      181 caatgctccg ttatattttt accgagaaag acatagtcaa cgtgcgattt gattattaca
      241 acaaaaaata gcaaaccccg gcaatcaaag ctcgtctgat ataatcgata tattctgtta
      301 aacgaaaaat aaatatgttt aaaaaattca aaccggtatt gttgtcattt tttgcacttg
      361 tatttgcctt ttggctggga acgggtattg cctatgagat taatccgcgt tggtttttga
      421 gcgatacggc aactgaagta cctgaaaatc caaatgcttt tgtggcgaaa cttgcccgcc
      481 tgttccgaaa tgccgacagg gcggttgtca tcgtgaagga atcgatgagg acggaggaaa
      541 gtcttgccgg agctgtggat gacggtccgt tgcagtcgga gaaggattat ctcgcgctcg
      601 ctgtccggct cagtcgtttg aaagaaaagg cgaaatggtt tcacgtaacg gagcaggaac
      661 atggggaaga ggtttggctg gattaccata tcggcgaggg cggtttggtt gcggtttcgc
      721 ttttgcaacg ctcgccggaa gcgtttgtta atgccgaata tctgtatcgg aacgatcgtc
      781 cgttttctgt aaatgtgtac ggcggaacgg ttcacggaga aaattatgaa acgacaggag
      841 aatatcgggt tgtttggcaa ccggacggtt cggtatttga taagtcgggg ggcgggaaaa
      901 tcggggaaga tgtttatgag cattgtctcg ggtgttatca gatggcccag gtatatttgg
      961 cgaaataccg ggatgttgcg aatgacgagc agaaggtttg ggacttccgc gaagagagca
     1021 accggattgc gtcggactct cgcgattatg tggattatca gaatatgcgg gaattgatgc
     1081 cccgagggat gaaggcgaac agtcttgtgg tcggctatga tgcggacggt ctgccgcaga
     1141 aaatctattg gagtttcgac aatgggaaaa aacgccagag tttcgaatat tatttgaaaa
     1201 acggaaatct ttttattgca caatcttcga cggtagcatt gaaagcggat ggcgtaacgg
     1261 cagatataca gacctatcat acgcaacaga cgtggttttt ggatggcggg cggattgtcc
     1321 gcgaagagaa acagggagac agactgcctg attttccttt gaacttggaa gatttggaaa
     1381 aagaggtgag ccgttatgca gaggctgcgg cgagacgttc gggcggcagg cgcgaccttt
     1441 ctcactgaac gcaaatgccg tctgaaaccg gttttcagac ggcattggag agtcaaacgc
     1501 tttttgcaat gccgtatatg ccgaatccga ttcgctacaa attgaaaacc acaatccaaa
     1561 aaacggaaat acacattaaa cggatctttt taccgaccgt atcatcccat aggttttcca
     1621 cacctttttt catccataaa ttcaaaccat ttaagtttgc atccttaccg acaattgcgg
     1681 cagcattatc cgcataacgg tcacccaaat tagccaaata acgccaaccg ccataggtat
     1741 cgccacggaa agcataacca tacaaggttt ggatttgttc tggtgttaaa acaacttcac
     1801 tctcattcat aataagatcc tttaaattat tttttacttg
//
LOCUS       DAWKOG010000220         1829 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.220, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000220 DAWKOG010000000
VERSION     DAWKOG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.220"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            325..1770
                     /gene="zwf"
                     /locus_tag="WJK66_002012"
     CDS             325..1770
                     /gene="zwf"
                     /locus_tag="WJK66_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEZ7579658.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVERLAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQTCENLAEIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALESRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWTNNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
BASE COUNT          504 a          416 c          482 g          427 t
ORIGIN      
        1 aaaggtatac cgtctgaaag tgggaagcgg gtgttattat agtacaaaat ttgtaatttt
       61 gttacaaaaa aaatcagaca tcgaaaagaa atttgattta aatcgcagaa tctttcgatt
      121 gttgtaacgg aataggggaa acctgcccga tttttggact aggtcaaata aattgtaaaa
      181 atgggaatag acaaaaagtg tagcggtgtg tagtattact actcattatg ccgcctgcct
      241 ttgtttccga tattgcgatg cagcgtgatg gggaaaacag gtttcagacg gcatcgaaaa
      301 attctttcct gacgagataa tgcgatgagt acacaaacaa attttgattt ggtgttgttc
      361 ggtgcgaccg gcgatttggc aatgcgcaaa cttttgccct gcctgtatca ggcgcacgtg
      421 gccggtttgc ttcatcccga aggtcgtatt ttgggtgtga gccgcagcga attggatacc
      481 gaaggttttc tggcgaaggt ggaaaccagt tccaaaatcc atgtgaaaga aaatttttcc
      541 gatgaggctt gggcatcgtt tgtcgaacgt ctcgcatatc tcaaagtcga tgttacgcag
      601 ccggacgatt ttgccgcttt gggcgatttg gtcaaagcgc gcaaagaaac cgacaatgtc
      661 gtcatctatc tttccaccgc gccgaaattc ttcgcgcaaa catgtgaaaa ccttgccgaa
      721 atcggtttga acgccgacaa cgtgcgtgtg gttttggaaa aaccgttggg tacggatttg
      781 gcttcttccc aacaaatcaa taccgatgtt gcgcgttatt tcaaagaagg gcaaatctac
      841 cgtatcgacc attatttagg taaagaaagc ctgcaaaacc tgctggcctt gcgttttgcc
      901 aatgtgatgt tcgagccgct ttggaacaat aaatacatcg aaagcgtgca gcttaccatc
      961 gccgagcagt tgggcgtgga agaacgcggc gagttttacg acattaccgg cgcgttgcgc
     1021 gatatggtgc aaaaccactt gatgcaaatg ctgtgcatga ctgcgatgga agcccccgcc
     1081 agcttggatg ccgacgcggt gcgcgatgaa aaagtcaaag tcatcaagtc attgaagccg
     1141 ctgaccgtcg aatctgtcaa tgaaaatgtc gtgcgcggac aatataccgc cgccaaaggc
     1201 atgaacggct atcttgaaga aatcaacgtt ccgcaagaca gctttaccga aacctacgtc
     1261 gccattaaag ccgaaatcga aaacgaacgc tggaagggcg ttcccttcta cctgcgtacc
     1321 ggcaagcgca tggcgggcaa agtggcggaa atcgttttga acttcaaaga tttgaacagc
     1381 catatttttg aaggcagtcg caccgcgccc aaccggctcg ttatcgagtt gcaaccatat
     1441 gaatccgtgc gcctctatac gcagatgaaa accccggggg caggaaataa ggtcgaaacc
     1501 gtgccgctgg caaccgattt gggcaaagca ttggaaagcc gccgcgcgga agcttacgag
     1561 cgcctgctgc tggatgtgat taacggcaaa ctcgctttgt ttaaccgccg cgacgaactt
     1621 gaagccgcgt gggaatatgt gatgccgatt ttggaaaact ggacaaataa caccacgccg
     1681 ccgcacggct acggcgcaca ctcgtggggg cctgaagccg cgcgcgaact attggcgcgc
     1741 gacggacaca agtggcacga agagcagtaa tacaataatg cgttcagacg gcatggggtt
     1801 tgaaatgccg tctgaacgta agtaaagca
//
LOCUS       DAWKOG010000221         1809 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.221, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000221 DAWKOG010000000
VERSION     DAWKOG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.221"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(188..844)
                     /locus_tag="WJK66_002013"
     CDS             complement(188..844)
                     /locus_tag="WJK66_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEZ7579659.1"
                     /translation="MKNKTKVWDFPTRLFHWLLAASLPFMWYSAKTGGDMLQWHTRVG
                     LLILFLLIFRLCWGIWGSDTARFSHFVRGWSGIRDYLKNGIPEHIQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAIH
                     IAAVAAYRVFKQKNLVLPMITGFKYIEGKTSIRFAGKTALAAALSVAALAAAAILLLS
                     "
     gene            909..1799
                     /locus_tag="WJK66_002014"
     CDS             909..1799
                     /locus_tag="WJK66_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEZ7579660.1"
                     /translation="MFPVFHLSGESRRRMLQTALRFPYVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVWAGFNQDALPFAVIAVFPEIMLDTADGLTKRQD
                     INAWVVGCGLGTGRAAVGTLAGILAEHTDKPVLLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIGAIFGATVVLKGHKTLVAASDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     LAGEIAPAARWLRNRITESM"
BASE COUNT          398 a          426 c          591 g          394 t
ORIGIN      
        1 aatcggttct gtactatccg tactgtctgc ggcttcgtct ccttgtcctg atttttgtta
       61 atccactata aaacggtttg ggacggaaaa acggcctgaa gccgtttcgg gcattcagac
      121 cgtttgcgcg gagaggggat gccgtccgaa gggcgaaaag ggcttcagac ggcattgatg
      181 tcgggtttca ggacaggagc aggatggcgg ctgcggcaag cgcggcaacc gataatgcgg
      241 cggcaagcgc ggttttgccg gcaaagcgga ttgaggtttt gccttcgatg tatttgaagc
      301 cggttatcat cgggaggacg aggtttttct gtttgaacac gcggtatgcg gcgacagcgg
      361 cgatatgaat ggcggaaaaa acggcgagca gcttgaaaaa gttgaggtgg attttccgca
      421 taaggctgcc tgtatgttca gaaaccaaat ggttgaggta gccgttggtg ctgaaggtgt
      481 tttcatcggc ggcaaaaagc cccgtgccga cttgaaatga gacggcggcc aaaagcgcga
      541 cgaccatcag tgcgcccaag gggttgtgtc cgggctggat atgttcggga ataccgtttt
      601 tcagataatc cctgatacct gaccatccgc ggacgaaatg ggaaaaacgg gcggtatcac
      661 tgccccaaat gccccagcag aggcggaata tgagcaggaa aaggataagc agcccgacgc
      721 gcgtgtgcca ttgcagcata tcgccgccgg ttttcgcgct ataccacata aagggtaggg
      781 atgcggcaag cagccaatgg aaaaggcggg tggggaagtc ccagactttg gttttgtttt
      841 tcataatcgg tttccggcgg tagaatcggt ttgttttcga gccttaattt taaccgattg
      901 gaggggaaat gtttcccgtt ttccatcttt caggcgaaag ccgccgccgg atgcttcaga
      961 cggcattgcg ttttccctat gttttcaaag cccgtgcgga agattcgcac aaagggactt
     1021 tcggcacgct cgccgtagtc ggcggatcgg cagggatgag cggcgcgccc gtattggcgg
     1081 catcggcggc gatgtatctc ggctgcggca aggtgtgggc gggtttcaat caggatgcgc
     1141 ttccgtttgc cgtcattgcc gtttttcccg aaattatgtt ggacacggca gacggtttga
     1201 ccaagcgtca agatataaac gcctgggttg tcggttgcgg cttgggtaca ggtagggcgg
     1261 cggtcggaac gcttgccgga attttggcgg aacacacgga caagcccgtc cttttggatg
     1321 cggacgcgct gaacatatta tcaaccgatg ccgaaacccg aaatctggcg cgcgggtgta
     1381 aaaacctgat tttaacgcca caccccgccg aagccgcgcg cctgcttgga acgacggttg
     1441 cacaggttca ggcggatcgg acggcggcag tgaggaagat aggggcaatt ttcggcgcaa
     1501 ccgtggtttt aaaggggcac aaaacattgg ttgctgcgtc cgatacggaa atctatgtca
     1561 acgaaagcgg caacgcggga ttggcaacgg cgggcagtgg cgacgtattg ggcggcatca
     1621 tcggcagtct gctcgcacag ggcgtgccgg tttttgaagc cgcctgcgcg ggcgcgtggc
     1681 tgcacggcgc ggcggcggat gtcataaaag aatcggcagg cattgcggcg gggctgttgg
     1741 ctggcgaaat cgccccggcg gcaaggtggc tgcgcaaccg gataactgaa agtatgtaag
     1801 aagatatag
//
LOCUS       DAWKOG010000222         1799 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.222, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000222 DAWKOG010000000
VERSION     DAWKOG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.222"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            319..1485
                     /locus_tag="WJK66_002015"
     CDS             319..1485
                     /locus_tag="WJK66_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEZ7579661.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNIQLQLTEPL
                     PNIQPQVTKRKSRIRTKISDFGSFIGFKGSEDLGEGLKAVWQLEQDVSVAGGGASQWG
                     NRESFIGLAGEFGTLRAGRVANQFDDASQAIDPWDSNNDVASQLGIFKRHDDMSVSVR
                     YDSPEFSGFSGSVQFVPAQNSKSAYTPAHFVQNKQNQRPTLVPAVVGKPGSDVYYAGL
                     NYKNGGFAGNYAFKYAKHANVGRDAFELFLIGSATSDEAKGTDPLKNHQVHRLTGGYE
                     EGGLNLALAAQLDLSENGDKAKTKNSTTEIAATASYRFGNAVPRISYAHGFDLIERGK
                     KGENTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHKF"
BASE COUNT          460 a          416 c          458 g          465 t
ORIGIN      
        1 gtttttgtta atccactata aagtgccgcg tgtgtttttt tatggcgttt taaaaagccg
       61 agactgcatc cgggcagcag cgcatcggcc cgcacgaggt ctgcgcttga attgtgttgt
      121 agaaacacaa cgttttttga aaaataagct attgttttat atcaaaatat aatcattttt
      181 aaaataaagg ttgcggcatt tatcagatat ttgttctgaa aaatggtttt ttgcgggggg
      241 ggggggtata attgatgaca tatcgggtgt ttgcccgatg tttttaggtt tttatcaaat
      301 ttacaaaagg aagccgatat gcgaaaaaaa cttaccgccc tcgtattgtc cgcactgccg
      361 cttgcggccg ttgccgatgt cagcctgtac ggcgaaatca aagccggcgt ggaaggcagg
      421 aacatccagc tgcagttgac cgaaccgctc ccaaatattc aacctcaggt tactaagcgc
      481 aaaagccgca tcaggacgaa aatcagcgat ttcggctcgt ttatcggctt taaggggagt
      541 gaggatttgg gcgaagggct gaaggctgtt tggcagcttg agcaagacgt atccgttgcc
      601 ggcggcggcg cgtcccagtg gggcaacagg gaatccttta tcggcttggc aggcgaattc
      661 ggcacgctgc gcgccggtcg cgttgcaaat cagtttgacg atgccagcca agccattgat
      721 ccttgggaca gcaataatga tgtggcttcg caattgggta ttttcaaacg ccacgacgat
      781 atgtcggttt ccgtacgcta cgattccccc gaattttccg gttttagcgg cagcgtccaa
      841 ttcgttccgg cccaaaacag caagtccgcc tatacgccgg ctcattttgt tcagaataag
      901 caaaatcagc ggcctactct cgttccggct gttgtcggca agccggggtc ggatgtgtat
      961 tatgccggtc tgaattacaa aaatggcggt tttgccggga actatgcctt taaatacgcg
     1021 aaacacgcca atgtgggccg tgatgctttt gagttgttct tgatcggcag cgcgacgagt
     1081 gatgaagcca aaggtaccga tcccttgaaa aaccatcagg tacaccgcct gacgggcggc
     1141 tatgaggaag gcggcttgaa tctcgccttg gcggcccagt tggatttgtc tgaaaatggc
     1201 gacaaagcca aaaccaaaaa cagtacgacc gaaattgccg cgactgcttc ctaccgcttc
     1261 ggtaatgcag ttccacgcat cagctatgcc catggtttcg acttgatcga acgcggtaaa
     1321 aaaggcgaaa ataccagcta cgatcaaatc atcgccggcg ttgattatga tttttccaaa
     1381 cgcacttccg ccatcgtgtc tggcgcttgg ctgaaacgca ataccggcat cggcaactac
     1441 actcaaatta atgccgcctc cgtcggtttg cgccacaaat tctaaatatc ggggcggtga
     1501 agcggatagc tttgtttttg acggcttgca ttcattcttc gattgcaatc tgactgccaa
     1561 tctgcttcag ccccaaacaa aaatccggat acggaagaaa aacggcaatt aaagacagca
     1621 aataccgtct gaaagatttt cagacggtat ttcgcatttt cggtttggtt tgcacatata
     1681 gtgggggact gttagttttt gcacaaggaa caaatagagt aaaaaaaacg ctgaaatctc
     1741 ggaaagacgt ggatttcggc gtttttttgc atccggaaaa gttacgccag ctttttcac
//
LOCUS       DAWKOG010000223         1733 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.223, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000223 DAWKOG010000000
VERSION     DAWKOG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.223"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            356..1660
                     /locus_tag="WJK66_002016"
     CDS             356..1660
                     /locus_tag="WJK66_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEZ7579662.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDATAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          356 a          432 c          510 g          435 t
ORIGIN      
        1 aattaaaaat ttttaatata ttaatcaata aattaatttt ataaaataaa aatattgtca
       61 acaatatttt gccttatcgc ccaaacctct gtatattttc ctacagtaaa ttgttgacaa
      121 tccatacgcc cacatatgcg ccgcctaagg ataaatcctc ccgccggaca acgggtgcaa
      181 gggattggat gcgatatttc catattcaaa caagggattt gtttcacgca cagggcggca
      241 catcggcaaa atccccgcgc cgccccggtc cagcagggct tgcgtccctc ccggacaagc
      301 cccgaccccg cctttccgaa agacgggctc aaccattaag gaaactttaa tcaaaatgaa
      361 aaaacacata tgggcggcat ctttgctgcc ggcatcccta tcggcagaac ctttaaactg
      421 gtggaagcct tattccgccg tcaattcggg cgataccgcc tgggtgatga ctgcggctgc
      481 cttggtactg ttgatgacgc ttcccgggct ggctttattc tacggcggta tggtgcggaa
      541 aaaaaacctg ctctcgacga tgatgcacag cttttccatc gcgacattgg tgggcatcct
      601 ttgggtcgcc gtcggctatt ctttagcgtt cacgccggga aatgccttta tcggcggttt
      661 ggggcgtgta tttttaagcg ggatgcagat agacgctacc gcacagatgc tgaccgtgtc
      721 gcccaatgcg ccgactgttc ccgaaccggt atttatgttt tttcagatga cgtttgccat
      781 tatttcgacc gccattatta ccggcgcgtt tgccgaacgg atgaaatatt cggcaatgat
      841 gctgttttcg ggcatatggt ttttattggt ttatgtgccg ggcgcgcatt gggtgtgggg
      901 cggcggcttt atgagcaagg gcggcgtatt ggattatgcc ggcggtacgg tggtgcacat
      961 caatgccggt atcgcgggac tcgtcgccgc cttggttttg ggcaggcgca taggctacgg
     1021 gcgcgaggcg atgcctccgc acaatatggc gatgacactg atcggcgcgg caatgttgtg
     1081 gttcggctgg ttcggcttta acgccggatc ggcgcttgcg gcagacgcgg cggcgggtat
     1141 ggcgatggcg gtaacgcagg tgtcggccgt attcggcgcg gcaggctggc ttgcctgcga
     1201 aaaaatagcg ggacacaaac cttccgcttt ggggctggct tccggcgcgg tttccggtct
     1261 ggtcggcatc acccctgccg ccggctttac cggcccgtcg ggcgcggccg ccatcggtat
     1321 attgactgcc gccgcgtgct ttgtgtccgt caccgtcgtc aaacacaaat tgcgttacga
     1381 tgattctttg gacgctttcg gcatacacgg attcggcggg ctggtgggcg gaatattgac
     1441 cggcatcttt ttcgacaacc gcattttcgg cggggatgcg gcagtttggc agcagttgtg
     1501 gatacaggta aaagacgggt tcgttatggc ggcatacagc gggctgatga gttgggcgat
     1561 tttgaaggtc gtggggaaaa tctgcggcgg cctgcgcgtc ggcaaggatg tcgaacgcga
     1621 aggtttggat ctgaatatcc acggcgaacg cgtggaataa gggcggttat gccgtctgaa
     1681 gcctgaaaat cgggtttcag acggcatttt tcacgtttgc cgccgatgga taa
//
LOCUS       DAWKOG010000224         1733 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.224, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000224 DAWKOG010000000
VERSION     DAWKOG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.224"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..1556
                     /locus_tag="WJK66_002017"
     CDS             <1..1556
                     /locus_tag="WJK66_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ7579663.1"
                     /translation="IAVLDAYTGQDSSTLYYMSEEDALNIVKVTNDTYDHLAKNIYQN
                     LLFQTRLQPYLNQISFKMENDTFTLDFSGLVQAFNHVKETNPQKAFVDLAEMLAYGEL
                     RSWYEGRRLMADYVEEAKKAGKFEDYQKVLGQETVALLAKTSGTQADDILQNVGFGHN
                     KNVSLYGNDGNDTLIGGAGNDYLEGGSGSDTYVFGKGFGQDTVYNYDYATGRKDIIRF
                     TDGITADMLTFTREGNHLLIKAKDGSGQVTVQSYFQNDGSGAYRIDEIHFDNGKVLDV
                     ATVKKLVQQSTDGSDRLYAYQSGNTLNGGLGDDYLYGADGDDLLNGDAGNDSIYSGNG
                     NDTLDGGEGNDALYGYNGNDVLNGGEGNDHLNGEDGNDTLIGGAGNDYLEGGSGSDTY
                     VFGEGFGQDTVYNYHVDKNSDTMHFKGFKAADVHFIRSGSDLVLSASEQDNVRISGFF
                     YGENHRVDTFVFDDAAISNPDFAKYINAGNNLVQSMSVFGSNTAATGGNVDANIQSVQ
                     QPLLVTPSA"
BASE COUNT          499 a          352 c          414 g          468 t
ORIGIN      
        1 gcattgccgt gcttgatgcc tacacggggc aggattccag cacactctat tacatgagcg
       61 aagaagacgc gcttaatatc gtcaaagtaa ccaacgatac atacgaccat ctcgccaaaa
      121 acatctacca aaacctgtta ttccaaaccc gtttgcagcc atatttgaat caaatcagtt
      181 tcaaaatgga aaatgatacg ttcactttgg attttagtgg tcttgttcaa gcatttaacc
      241 atgtcaaaga aactaatccg caaaaagctt ttgtggattt ggccgagatg cttgcatatg
      301 gcgaacttcg ttcttggtat gaaggccgaa gactaatggc cgattatgtg gaggaggcaa
      361 aaaaagcagg taaatttgaa gattaccaga aagtgttggg tcaggagacc gttgcattat
      421 tagctaaaac atcgggtacg caagcagatg atatcctgca aaatgtaggc tttggtcata
      481 ataaaaatgt ttctttatat ggtaatgacg gcaacgacac tctaatcggc ggtgccggta
      541 atgattactt ggagggcggc agcggttcgg atacttatgt cttcggcaaa ggcttcggtc
      601 aggatacggt ctataattac gactacgcta ccggacgcaa agacatcatc cgctttaccg
      661 acggtattac agccgatatg ctgactttta cccgagaggg caaccatctt cttatcaagg
      721 caaaagacgg cagtggacaa gtgactgttc agtcctattt ccagaacgat ggctcaggtg
      781 cttaccgtat cgatgagatt catttcgata acggcaaagt actggatgtt gccactgtca
      841 aaaaactggt acagcaatcc accgacggtt cggacagatt gtatgcctac caatccggaa
      901 ataccttaaa tggcggattg ggcgatgact atctgtacgg tgccgacggg gatgacctgc
      961 tgaatggtga tgcaggcaac gacagtatct acagtggcaa tggcaatgat acgctcgatg
     1021 gaggagaagg caacgacgcc ctgtacggct ataatggtaa cgatgtactg aatggtggcg
     1081 aaggcaatga tcatttgaac ggcgaagacg gtaacgacac tctgatcggc ggtgccggta
     1141 atgattactt ggagggcggc agcggttcgg atacttatgt cttcggcgaa ggcttcggtc
     1201 aggatacggt ctataattac catgtggata aaaactctga cactatgcac tttaaaggat
     1261 ttaaagcagc agatgttcat tttatccgtt ccggaagtga tttggtgctt agcgcttctg
     1321 aacaagacaa cgtacgtatt tccggattct tctatggtga aaaccatcgt gtagatacat
     1381 ttgtctttga tgatgcagct atcagtaatc cagattttgc caagtatatt aatgctggca
     1441 ataatttggt acagtctatg tctgtgttcg gttctaatac tgctgcgaca ggaggaaatg
     1501 tggatgccaa tatacaatcc gtacagcagc cgttattggt aacgccatct gcataaggag
     1561 cctaattaca ttcatggctt aaactgaaaa acagcaatca agtttatttt gattgctgtt
     1621 tttcttaata ttgggataag ggtcgtattt taattaacct taatcggtgc acttctagca
     1681 atatagtgga ttcacaaaaa ccagtacagc gttgcctcgc cttaccgtac tat
//
LOCUS       DAWKOG010000225         1710 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.225, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000225 DAWKOG010000000
VERSION     DAWKOG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.225"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            61..1434
                     /locus_tag="WJK66_002018"
     CDS             61..1434
                     /locus_tag="WJK66_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ7579664.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQSY
                     IKNLPLRHTVLFGGMNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPKQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTIQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKTEMREPRQRNRYESARAQREKNTRPETTANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
     gene            1614..>1710
                     /locus_tag="WJK66_002019"
     CDS             1614..>1710
                     /locus_tag="WJK66_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEZ7579665.1"
                     /translation="MSTQFDVAVIGAGPAGSVASALLRKKGYSGLN"
BASE COUNT          441 a          560 c          405 g          304 t
ORIGIN      
        1 tccccatcct atcggtttcc cgcttcagac ggcataaggt ctgaaagaaa gactacaatt
       61 atgagtaatc cattttcctc tttaggtttg ggtacggaac tcgtttccgc actgaccgcg
      121 caaggttacg aaaacccgac gcccatccaa gccgccgcca ttccaaaagc actcgccggt
      181 catgatttac tagccgccgc ccaaaccggc acgggcaaaa ccgccgcctt tatgctgccc
      241 tcgctcgaac gcctcaaacg ttacgccacc gccagcacct cgcccgcgat gcaccccgtg
      301 cgtatgctcg tcctcacccc cacgcgcgaa cttgccgacc aaatcgacca aaacgtgcag
      361 tcctacatca aaaacctgcc gcttcgccac accgtcttgt tcggcggtat gaatatggac
      421 aaacagaccg ccgatttgcg tgccggctgc gaaatcgtcg tcgccaccgt cggacggctg
      481 ctcgaccacg tcaaacaaaa aaacatcagt ttgaacaaag tcgaaatcgt cgttctggac
      541 gaagcagacc gcatgttgga tatggggttt atcgacgaca tccgcaaaat catgcagatg
      601 ctgcccaaac aacgccaaac cctgctcttt tccgctacct tctccgcacc gatacgcaaa
      661 ctggcgcaag acttcatgaa cgcgcccgaa accgtcgaag tcgccgcgca aaacaccacc
      721 aacgccaacg tcgaacaaca catcatcgcc gtcgatacca ttcagaagcg caacctgctc
      781 gaacggctga ttgtcgattt gcatatgaac caggtcatcg tgttctgcaa aaccaaacaa
      841 agcgtcgacc gcgtaacgcg cgaactggtg cgccgcaacc tgtccgcaca ggcgatacac
      901 ggcgaccgtt cccaacaaag ccggctcgaa acactcaacg ccttcaaaga cggcagcctg
      961 cgcgtcctcg tcgccaccga catcgccgcg cgcgggctgg acattgccga actgcccttc
     1021 gtcatcaatt acgaaatgcc cgcccagccc gaagactacg tccaccgcat cgggcgcacg
     1081 gggcgcgcgg gcgcggacgg cgtggcgatt tccctgatgg acgaatccga acagaaaatg
     1141 tttgaatcca ttaaagagct gaccggcaac aagctgctca tcgagcgcat cgagggcttc
     1201 gagccgcaat ggtgggaaca gggcggcgca aaaccggaaa aaaccgaaat gcgcgaaccg
     1261 agacaacgca accgctacga atccgccagg gctcagcgcg aaaaaaatac ccgaccggaa
     1321 accacggcaa acgatgcggg cgcggcttgc ggaaaaattg ccggacgcag ccgccgaagc
     1381 cgtcgggaac accggacgtg cgccctgctc caaccgcgtt acggcgtaaa ataaccctga
     1441 aaatcaaatg ccgtctgaac atttcccgtt tcagacggca tttttcaaac cggactgacg
     1501 cattgggagc aaccgcccgc accggataaa tttcttccgc aaacagtttc agacggcatt
     1561 tgctgcctgt acaatatcat ttccttttcc actcaaacca tcaggatttc ccaatgtcca
     1621 cccaattcga tgtcgccgta atcggcgcag gtccggcagg ctcggtcgca tccgccctgc
     1681 tccgcaaaaa aggttatagt ggattaaatt
//
LOCUS       DAWKOG010000226         1706 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.226, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000226 DAWKOG010000000
VERSION     DAWKOG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.226"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..753
                     /gene="mafB"
                     /locus_tag="WJK66_002020"
     CDS             <1..753
                     /gene="mafB"
                     /locus_tag="WJK66_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ7579666.1"
                     /translation="RSIKLNPTDTRSIRQRISDNYNNLGSNFSDRADEANRKMFEHNA
                     KLDRWGNSMEFINGVAAGALNPFISAGEAVDQWMQENPNAAETVEALVNVLPFAKVKN
                     LTKAAKPGKAAVSGDFSDSYKHNTASRLSQSVDGEMFQTRNVDFKAKSIGTKIHDGAQ
                     GKHISGHRNYIEGKSTLNQNINPQELLNGIHSGAYPVISKGARGNPVVDFGYPIGSDG
                     KSGLSTNFGTIHSGKNGVHIVPANPKTIKKVQ"
     gene            756..1073
                     /locus_tag="WJK66_002021"
     CDS             756..1073
                     /locus_tag="WJK66_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579667.1"
                     /translation="MNILPSWLRVGMNIAMLGMIHSDIRLITVDYEEGRRFLKIKNYL
                     SREAITEDHADMEYLITELWSMCGEYFDEADFECIYSNHSSMELNQINGAVFRRKELI
                     SQA"
     gene            1207..>1706
                     /locus_tag="WJK66_002022"
     CDS             1207..>1706
                     /locus_tag="WJK66_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hint domain-containing protein"
                     /protein_id="HEZ7579668.1"
                     /translation="MVKTADGYKAIARIRTGDRVFAKDEASGKTGYKPVTARYGNPYQ
                     ETVYIEISDGIGKIQTLVSNKIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWHTYFVKGNQAERKGFGFIMRVRQEKHHLPPYMGMIQKHMQQQR
                     NWDIEK"
BASE COUNT          556 a          381 c          375 g          394 t
ORIGIN      
        1 cgcagcatca aactcaatcc gaccgacacc cgcagcatcc ggcaacgcat atccgacaac
       61 tacaacaacc tcggcagcaa tttctccgac cgcgccgatg aagccaacag aaaaatgttc
      121 gagcacaatg ccaagctcga ccgctggggc aacagcatgg agtttatcaa cggcgtcgcc
      181 gccggcgcgc tcaacccctt tatcagcgcg ggcgaagccg ttgaccagtg gatgcaggaa
      241 aaccccaatg ccgccgaaac cgtcgaagcc ctggtcaacg tcctgccgtt tgccaaagtc
      301 aaaaacctga caaaggcggc aaaaccgggg aaggctgcgg ttagtgggga tttctcagac
      361 tcctacaagc ataacactgc ttcaagatta tctcagtctg tagatggaga aatgtttcaa
      421 acccgcaatg ttgattttaa agcaaaatct attgggacta aaattcatga tggagctcaa
      481 gggaaacata tttcaggaca tagaaactac attgaaggta agagtacttt aaatcaaaac
      541 attaatcctc aagaattgtt gaacggaata cattcaggtg cttatccagt tatttctaaa
      601 ggagcaagag gaaatcctgt tgttgatttt gggtatccta taggcagcga tgggaaatca
      661 ggattaagta ccaattttgg tacgattcat tcaggtaaaa atggagttca cattgttccg
      721 gctaacccta aaaccattaa aaaggtgcaa tagttatgaa tatattacca agctggctgc
      781 gagtcggtat gaatatagca atgctgggca tgatacactc agatatcagg ttaattaccg
      841 tagattacga ggaaggaaga aggtttttaa aaatcaaaaa ttatttatca agagaagcca
      901 tcacagaaga ccatgcagat atggaatatt tgattacaga gttatggtct atgtgtggag
      961 aatattttga tgaagctgac tttgaatgta tttattctaa tcattcttct atggagttaa
     1021 accaaataaa tggtgcagta ttcaggagaa aggaattaat ttcgcaagcg taggttaaaa
     1081 aaccaacaat cacaatgtct tctgaaaccg tgtttaattt tcagacggca tttccttcat
     1141 ttgaaatagg atattgagaa ctgagttctt caaaaatcct acacctgctc cttccacggc
     1201 agcaccttgg tcaaaacggc agacggctac aaagccattg cccgtatccg aaccggcgac
     1261 cgcgtcttcg ccaaggacga ggcaagcgga aaaacgggat acaaacccgt taccgcccga
     1321 tacggcaatc cgtatcaaga aaccgtttac attgaaattt cagacggcat cggtaaaata
     1381 cagaccctcg tttccaataa aatccacccg ttttacagtc aaggaaagtg gatacaggca
     1441 ggtcgtctga aaaaaggcga caccctgctt tccgaaagcg gtgcaaaaca gacggttcaa
     1501 aacattaccc tcaaacagca gccgctcaaa gcctacaatc tgaccgttgc cgactggcat
     1561 acctacttcg tcaagggtaa tcaggcggaa cggaaggggt ttgggttcat aatgcgtgtc
     1621 cgccaagaaa aacaccatct acccccgtat atgggaatga ttcagaagca tatgcagcag
     1681 caaagaaatt gggatataga aaaata
//
LOCUS       DAWKOG010000227         1655 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.227, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000227 DAWKOG010000000
VERSION     DAWKOG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.227"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            186..602
                     /locus_tag="WJK66_002023"
     CDS             186..602
                     /locus_tag="WJK66_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HEZ7579669.1"
                     /translation="MHLRRILPPTAEALMRSRYSAYVLHLIDYIIATTVPAQQAFLDA
                     AELMQWSIETEWLGLNVIAHRNFGKQHAQVEFEAYFRDGQHRSAHHELSGFVNIGGQW
                     YFIDPTVPHPAMKQPCICGSGKKFKACCGKYLKPVA"
     gene            complement(820..1614)
                     /locus_tag="WJK66_002024"
     CDS             complement(820..1614)
                     /locus_tag="WJK66_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEZ7579670.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSCRKEDGSMLSEADIAAQT
                     AFAAALPLLIDCPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
BASE COUNT          420 a          489 c          394 g          352 t
ORIGIN      
        1 gtttgcgcct aaaaggcggc ccggatgcct gattatcggg tatccgggca ggattaaggg
       61 ggtattgggg taaaattagg aggtatttgg ggcggaaata gctgaaaacc tgtgtttggg
      121 tttcggctgt cgggagggaa aggaattttg caaaggtctc gggctatgcc ggctgctgcc
      181 gcccattgca ccttcgccga atcctgccgc ccaccgccga agccctcatg cgttcgcggt
      241 acagcgcgta tgtgctgcac cttatcgact acatcatcgc tactaccgtt ccggcacaac
      301 aggcattcct cgatgccgcc gaactgatgc aatggagtat agaaaccgaa tggctgggct
      361 tgaatgtcat cgcacaccgc aacttcggca aacagcacgc ccaagtcgaa tttgaagcct
      421 acttccgaga cggacaacac cgatccgcgc atcacgaact gtccggcttc gtcaacatcg
      481 gcggacaatg gtattttatc gatcccaccg ttccgcatcc tgcgatgaaa caaccctgca
      541 tttgcggatc aggcaaaaaa ttcaaagcct gctgcggcaa atatctgaaa cctgtcgcat
      601 aaaaatgccg tctgaacgtt cagacggcat tttcaacgtg caaaaaaaaa ccattcatac
      661 caagggtaag tatgaatggt caatacattg cgggaaaacg tcttacttgc tgcactgccg
      721 aaaagggaga aacggcagcg gtaatcagcg gaaaggattg tacccgaatt aatgttaaga
      781 aacgttaatc gtgaaatata ttaacaaacc tgttgaagcc tattggtttt cccgtatcca
      841 cccgacccag cgttcaaaca gcttcggttc gagcgcggca acgaccgagc gtttgaacac
      901 gtgttcacca ctccaaaacc cgtcgccttc caaagtcgtc agcctgccgc ccgcctcctc
      961 gaaaatcaac gcgccggcgg cataatccca cagcttctgc ccgccgtgga cataaacatc
     1021 ataacgcccg cacgccagat aacaccagtc caacgtactg ctgcccatac tccgtatcgt
     1081 tccaaaaggc gcgagcgtac tcatgcggct ggacagtttg cccgaacgca aatatttgat
     1141 ttccacgccc gcaatcgcct cattgagttt tttatccacg agacgcaaag gcaggcgggt
     1201 tccattaaga aacgcaccct gcccccgttc ggcataaaaa cattcgccgc tgaccgggtt
     1261 gtagattacg cccaattccg cacgcccgtt gcggacaaac gccaccgaca ccgcaaaatg
     1321 cggcagcccg ttgacaaaat tgttcgtccc gtcgatcgga tcgacaatcc acagcccctt
     1381 ttcccccgaa tattgttccc acaaagccga ctgttcctgc cgcgacattt cctcgcccaa
     1441 catcgggcaa tcgatcagaa gcggcaacgc ggcggcaaat gccgtctgcg cggcaatgtc
     1501 cgcctcgctc aacatcgaac catcttcctt gcggcaagac ggcgtattca aaaaacgcgg
     1561 cataatttcg gtttgcgcga tatggcgcac gactttctgc aaacggtgta acacttccta
     1621 ctgtcctcat attttgaact tgcggcgcgc gaacg
//
LOCUS       DAWKOG010000228         1563 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.228, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000228 DAWKOG010000000
VERSION     DAWKOG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.228"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(54..1538)
                     /gene="tldD"
                     /locus_tag="WJK66_002025"
     CDS             complement(54..1538)
                     /gene="tldD"
                     /locus_tag="WJK66_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEZ7579671.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVRQFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRESYASAPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
BASE COUNT          292 a          461 c          477 g          333 t
ORIGIN      
        1 aatgtaaaag gccgcctgaa aacctttcag acggcatcgt gccggattcc gcgtcagatt
       61 gcgctgccgc cgacggtcag cccggcatcg atacgcaggg tcggctgccc cacgccgacg
      121 gggacgctct gcccttcttt gccgcacacg ccgacaccgc tgtccaacgc ggtatcgttg
      181 cctatcatcg aaacgtgttt cagcacttcg ggcccgttgc cgataatggt cgcgcctttg
      241 acggggtatt gcagcctgcc gccttccacc caccatgctt cggacgcgct gaacacaaat
      301 ttgccgctgg taatgtccac ctgtccgccg ccaaagttga cggcgtaaat gcctttgtcg
      361 atggacgcga tgatttcttc cggctcatag ctgccatttt ccataaaggt attggtcatg
      421 cgcggcatag gggcggaagc gtaactttcg cggcggccgt tgccggtgga ttgcgtaccc
      481 gtcaggcggg cgttggtttc gtcctgcata tagccgacca aaataccgtc ttcaatcaat
      541 acggtgcggc gggtttcgtt gccttcgtcg tcgatattga gcgaaccgcg ccggccggca
      601 atatcgccct gatcgacgac ggtaacgcct ttggcggcga cgcgctcgcc gattctgccg
      661 gaaaagacgc tggtcccctt gcggttgaaa tcgccttcca aaccgtgtcc gaccgcttcg
      721 tgcagcaaca cgcccggcca gccgttgccc aaaacgacgg tcatttcgcc ggcgggcgcg
      781 gggcgggatt cgaggttggt gagtgcctgt ttgacggcgg catcgacaaa ctgccgcacc
      841 aagttttcat cgaaataagc caaatcataa cgtccgccgc cgcccgcgct gccctgttcg
      901 cggcgttcgc cctgtttggc gataacggta acgttcaggc gcaccatcgg gcggatgtcg
      961 gcggcgtgtt tgccgtccag acgggcgagg taaaccatgt cgtattcgca ggtcaaaccg
     1021 gccatcactt gcacgatgcg cggatcggcg gctttggcga ttgcttccac tttgttcaac
     1081 agcgcgactt tggcggcgga atcgaggccg gcaatggggt cggacgcgga acaaaccggc
     1141 ttgccgcgcg tttcagacgg cattttggcg gacaccttgc cgcctgccgc cccaatcgcg
     1201 cggacggcgc gggcggaacg gtttatcgaa tcgatgcaca ggctgtcggc gtaggcaaag
     1261 gcggttttgt cgcccgaaac ggcgcgcacg cccacgccct gattgatttg gaagctgccc
     1321 gatttgacga tgccctcttc caaatgccag ctttcataag cggtgcgctg gcagtagatg
     1381 tcggcgtaat cgacgtggtg cgcgccgatg atgcacaggc ttttggcgag cagttcgggg
     1441 gaaaggcggt tggcttcgag cagccgcgcc tgtacggcgg aataggtcgg atgcatagtg
     1501 tcggcgcata aaaaatcagg ggcttgatta tacggcattt gttatatagt ggattaacaa
     1561 aaa
//
LOCUS       DAWKOG010000229         1491 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.229, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000229 DAWKOG010000000
VERSION     DAWKOG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.229"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(132..1121)
                     /gene="porB"
                     /locus_tag="WJK66_002026"
     CDS             complement(132..1121)
                     /gene="porB"
                     /locus_tag="WJK66_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HEZ7579672.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVETSRSLDYQDGQV
                     YSVETGTGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQSFIGLKG
                     GFGKLRVGRLNSVLKDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPEFAGLSGS
                     VQYALNDNAGRHNSESYHAGFNYKNGGFFVQYGGAYKRHQDVDDVKIEKYQIHRLVSG
                     YDNDALYASVAVQQQDAKLVEDNSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGSVDD
                     AKRDNTYDQVVVGAEYDFSKRTSALVSAGWLQEGKGENKFVATAGGVGLRHKF"
BASE COUNT          378 a          339 c          382 g          392 t
ORIGIN      
        1 aaacagaaaa aagccaaaca gaaaaaagcc aaacagaaaa aagccaaaca gaaaaaagcc
       61 aaacagaaaa aagccaaaca gaaaaaagcc tgtctggcga caggcttttt gttgatacca
      121 atctttgcag attagaattt gtggcgcaga ccgacaccgc cggcagtcgc tacgaatttg
      181 ttttcgcctt tgccttcttg caaccaaccg gcagaaacca aggcagaagt gcgtttggag
      241 aagtcgtatt ccgcaccgac aaccacttgg tcgtaagtat tgtcgcgttt tgcatcatca
      301 accgagcctt tgaagccgtg ggcgtaagaa acgcggggcg ttacgttgcc gaagcggtat
      361 gccaaggtag cggcaacttc ggtttgagag ttgtgcgaat tgtcttcaac cagtttcgcg
      421 tcttgttgct gtacggctac ggaagcgtat agggcatcat tgtcgtaacc gctgaccaaa
      481 cggtgaatct ggtatttctc aatcttcacg tcatccacat cctgatgtct tttataggca
      541 ccgccatatt gcacgaagaa gccgccgttt ttgtagttga agccggcgtg gtaagattcg
      601 ctgttatgtc tgcctgcatt gtcgttaagc gcgtattgta cgctgccgct gaggccggca
      661 aattcgggag aatcgtagcg tacggaaatg aggcgtgcct cgggttcggc aattttgttt
      721 acacccaaat agtcgctttt gctatcccaa ggattgatgt cgccggtgtc tttcaggacg
      781 ctgttcaaac ggccgacgcg caatttaccg aagccgcctt tcaagccgat gaaggattgg
      841 cggttgcccc aaccggagtc agtaccggcg atagatgctt tttgctcaac ctgccaaatg
      901 gctttcaggc cgttaccgag gtcttcttgg cctttgaagc cgattttcga acccaaatca
      961 acgatgccgg taccggtttc aacgctataa acttgaccgt cctggtaatc tagggagcgg
     1021 gaagtttcta cgccggcttt gatggtgccg tacagggtaa cgtcagccat tgctgcaaca
     1081 ggaagggctg ccaaagtcag ggcaatcagg gattttttca ttgctgtatt ccttttttgg
     1141 ttaagaaatt taagcaggcc gggctttcca agccgcttag ctttgcattt accgccgagg
     1201 ggctcggcga ttacatgtct gaaactatag atgttcctgt ctgaaaaaac aaacttctgc
     1261 ctgtttttgt gtggaattcg atgtgcattt tgaaggacgg atagagatat tatgggtatt
     1321 ttttgtttta taacatatgg ttatttaaat tttttaagat ttgcattttt acaacactta
     1381 ctcgggaggg tattggaggg cattgcaaac cgggggttat aaagacggca aaaaacgcac
     1441 acgcggtttc tttcggaacg ggcggatgca aaacccgccc ctgcggcagg a
//
LOCUS       DAWKOG010000230         1441 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.230, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000230 DAWKOG010000000
VERSION     DAWKOG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.230"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            25..1362
                     /locus_tag="WJK66_002027"
     CDS             25..1362
                     /locus_tag="WJK66_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ7579673.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTVLVALPVQLAEFYIGRTGGQNAVDSFRALRPSTQWLWVGRMGVSACFILLSF
                     YSVVGGWVLNYVVHSFTGAVHTGADFEALFGTTISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIAATIRQDEH
                     KRKKHTWLIGTAIFIVGIPSALSFGVWGEFKVFGKTIFDLWDYVISAVIMPIGALSVS
                     IFTAWIQDKQSVLKNAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFINSLDIL"
BASE COUNT          265 a          351 c          379 g          446 t
ORIGIN      
        1 tcttcacatt tcttaataaa ttttatgagt aaccatactt cttggtcgtc caaaatcggt
       61 ttcgtgcttg ctgcggcagg ttcggccatc ggtttgggcg cgatttggaa atttccttat
      121 acggcaggca ccaacggcgg cgcggtgttt ttcctgctgt ttttgatatt taccgtcttg
      181 gtcgccctgc ccgtccagct tgccgagttt tatatcgggc gcacaggcgg tcaaaacgcc
      241 gtcgattcct tcagggctct gcgtccgagt acgcaatggc tttgggtcgg gcgcatgggc
      301 gtttccgcct gctttatttt gctgtcgttt tacagcgtgg tcggcggatg ggtattaaat
      361 tatgtcgtcc acagtttcac gggggcggtt cataccggcg cggactttga agccttgttc
      421 ggcacgacga tttccaatcc ggcaggttcg ctgtcctatc aagcactgtt tatgctgatt
      481 acggtttggg tggtcaaagg cgggatttca gacggcattg aaaaggcaaa ccgttatctg
      541 atgccgggac tgtttatcct ctttattgcg ctggcaatcc gttcgctgac gctgccgggt
      601 gcaatggagg gcgtgtcttt cctgctcaaa ccgaattggt cgtactttaa agccgatacg
      661 atgattacgg ctttgggaca ggcgtttttt gccctgagca tcggcgtttc cgccatgatt
      721 acctacgctt cgtatttggg aaaagatcag gatatgttcc gttccggcca tacgattatg
      781 tggatgaacc tcttggtttc gctgcttgcc ggcctggtga ttttcccggc ggtgttcgcc
      841 ttcggttttg aaccgagcca ggggccggga ttgattttta tcgtattgcc cgcagtgttt
      901 atgaagatgc cgttcggtac ggttttgttt gcggtattta tgctgctggt cgttttcgcc
      961 acgctgactt cggcattttc gatgttggaa acggtcattg ccgcaaccat ccgccaagac
     1021 gagcacaaac gcaaaaaaca cacttggctt atcggcacgg ccattttcat tgtcggcatc
     1081 ccatccgcgc tgtctttcgg cgtatggggt gagtttaagg ttttcggcaa aaccattttt
     1141 gatttgtggg actatgttat ttccgccgtc attatgccga ttggtgcttt gagtgtttcc
     1201 atctttaccg cctggattca ggacaagcag tctgtgttaa aaaatgccgg cgcgggcagc
     1261 accgtaccac gggcagtgct gctgctgtgg ctgaatacct tgcgctacct tgccccgatt
     1321 gccattatta ttgttttcat caattctttg gacatccttt aaaagccatc caaacagcaa
     1381 aaatgccgtc tgaaagcctt tcagacggca tttttgcttc aggttcagcc tatttcgttc
     1441 a
//
LOCUS       DAWKOG010000231         1429 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.231, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000231 DAWKOG010000000
VERSION     DAWKOG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.231"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..178
                     /locus_tag="WJK66_002028"
     CDS             <1..178
                     /locus_tag="WJK66_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579674.1"
                     /translation="ALIAAKIDQAEISDSEKSWLKKELGKIKDTALSTLTENAINAIL
                     AATLITLLKKFVGL"
     gene            complement(175..681)
                     /locus_tag="WJK66_002029"
     CDS             complement(175..681)
                     /locus_tag="WJK66_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ7579675.1"
                     /translation="MAHPQEIREKLRRLYVSGEQTLETAALMCEIPQTTARAWKRADK
                     EKGDDWDKMRAAYTLAGGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLADAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPAFVEVLEPFG
                     VEVEKKFG"
     gene            complement(684..881)
                     /locus_tag="WJK66_002030"
     CDS             complement(684..881)
                     /locus_tag="WJK66_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579676.1"
                     /translation="MSDPILEALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTAITF
                     GALGGGVITVGWELLKAKMGL"
     gene            complement(878..1144)
                     /locus_tag="WJK66_002031"
     CDS             complement(878..1144)
                     /locus_tag="WJK66_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579677.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKQDAKADRDNIAASLGRIENKLEKVNEKLDRKADKS"
     gene            complement(1156..>1429)
                     /locus_tag="WJK66_002032"
     CDS             complement(1156..>1429)
                     /locus_tag="WJK66_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579678.1"
                     /translation="SRAREQEPFVIHVSDLLDKAGIDYAVRMDKDFQTTFHLEYPITN
                     YDTFKRAVWQTLGAYYCVCNDGDGLEIASNRPDGYAVRIVFGDVPV"
BASE COUNT          344 a          405 c          310 g          370 t
ORIGIN      
        1 ggctttaatt gcggcaaaaa tagaccaagc ggagatttct gattcagaaa aaagctggct
       61 gaaaaaagaa ttagggaaaa tcaaggacac tgcattaagt acgcttacgg aaaatgcaat
      121 taatgctatc cttgccgcca ccctcattac tcttttaaaa aaatttgtcg gcctctaacc
      181 aaacttcttc tccacttcca ccccaaacgg ctccaatacc tccacaaagg caggcaaatg
      241 tttggggtgt ttttcttgca caaacgccat caagaactca atcaattcca aagccgtcgc
      301 cagttttgac gtttccggca tcacacgcgc attggcggat acggttttcg taaacgcatc
      361 ggccaggctg gccaacagct tggcgcggtc ggacgggggc aaatcttcgg tactcgaatc
      421 ctgcagcatc gtcatcgtgc tgttgtactg caccataaaa ccggccaaca tcgcacggct
      481 caagtcctca ataccgccgc cggccaaagt gtaggcggcg cgcatcttat cccagtcgtc
      541 gcctttttcc ttatccgcac gtttccacgc acgcgcagtg gtctgcggga tttcgcacat
      601 caaggccgcc gtttccaaag tttgctcgcc gctcacatag agccgacgta acttttcacg
      661 gatttcttgc gggtgagcca taattacagt cccattttcg ctttaagcaa ttcccagccg
      721 accgtaatca cgccgccgcc cagtgcgccg aatgtaatgg ccgtgcgttt cgtgtcttgg
      781 cggatttgcg caatttccgc ctgcatttct ttctgatttt tcagcgtttg atcagtcttg
      841 ttttcaatac gcgccaaggc ttccaaaatc gggtcgctca tgatttgtct gctttcctgt
      901 ccagtttttc gtttactttt tccaacttgt tttcgatgcg tcccaacgaa gccgcaatat
      961 tgtcgcggtc tgccttggca tcttgcttgg tgtgataaga gagcttgacc gcgtgcagct
     1021 cctctttcag gtcgtcgata cgcttgtccg cctctttcag acggcctgaa atgccgttga
     1081 cccaaaacca aaatgccgcc gtcgcaatcg gccacagggt tctgaaacca aattcaaagt
     1141 ccatttaaaa cccctttaaa ccggcacatc gccgaatacg atacggacgg cgtaaccgtc
     1201 agggcgattg ctggcaatct ccagtccatc accatcatta caaacacagt aatacgcccc
     1261 caaagtttgc caaaccgcac gtttaaaggt gtcatagttc gtaattggat attcaaggtg
     1321 aaacgtcgtc tgaaaatcct tatccatgcg taccgcgtaa tcgataccgg ccttatccaa
     1381 caaatcggaa acatgaatga caaacggctc ttgctcgcgt gcgcggctt
//
LOCUS       DAWKOG010000232         1414 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.232, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000232 DAWKOG010000000
VERSION     DAWKOG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.232"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            186..1370
                     /gene="ubiM"
                     /locus_tag="WJK66_002033"
     CDS             186..1370
                     /gene="ubiM"
                     /locus_tag="WJK66_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HEZ7579679.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIECSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNITILTDTAVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAQPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
BASE COUNT          378 a          412 c          308 g          316 t
ORIGIN      
        1 accccacacc cgatttgata cacattaagc tgaaagtaaa aatccgcata caccctcccc
       61 tgcatatttc ttcaacagtg ggttttgata taatcgccta tctgttacag atagttcaaa
      121 cggcattcaa acccttacaa ataccgtctg aaatcatcga catccgtcta tgcaaaggat
      181 attttatgag tttacacagc gacatcctcg tcgtcggcgc aggcccggcg ggtttaagtt
      241 ttgcagccga acttgccggc agcggtttga aagttaccct gatcgaatgc agcccactga
      301 ctgttctgca aaatcctcca tatgacggac gcgaaatcgc cctgacccat ttttcccgcg
      361 aaatcatgca gcgtttgggt atgtgggaca aaattcccga aaacgaaatc taccctttgc
      421 gcgatgccaa agtgctgaac ggacgttccg actaccagct ccactttccc caaccgaccg
      481 aggcgcgcgg cgagcctgcc gactgtttgg gctatctgat ttccaaccac aatatccgcc
      541 gcgctgccta cgaagtcgta tcccaactcg acaacattac cattctgacc gatacggccg
      601 ttaaagaagt caaaacatct gacaacgaag cacaagtcat tttagaaaac ggcaaaatcc
      661 tgaccgcccg cctgcttttg gcggcagaca gccgtttttc gcaaacccgc cgacaactcg
      721 gcatttcttc agatatgcac gactacagcc gaaccatgtt cgtctgccgc atgaaacata
      781 ctctttccaa ccaacatacc gcttacgaat gcttccatta cgggcgcacc atcgcactgc
      841 tgccgctgga agaacacctg accaataccg tcattaccgt cgataccgat aaaatcaaca
      901 gcgttcaaaa cctttcgccc gaagaattgg cggcaagcgt aaaagaacag ctcaagggca
      961 ggctcggcga tatggaattg gtcagcagca tccaccatta cccccttgtc ggtatgattg
     1021 ccaaacgttt ctacggcaaa cgcagcgcgc tgatcggcga tgcggctgtc ggtatgcacc
     1081 ccgttaccgc acacggtttc aacttgggtc tgtccagcgc agatattttg gcaaaactga
     1141 tactcgaagc cgagcagcgc ggtcaggaca tcggcgcggc ttccttgttg gaaaaataca
     1201 gcagcaaaca tatgctgcat gcccagccgc tttatcacgg aaccaatatg atgctgaaac
     1261 tgtttaccaa tgagaccgcg ccggcaaaac tgctgcgcgg tttggtattg cgtgccggca
     1321 acaacttccc gccgctgaaa aaactgatta ccaaacaact gaccggttga aaactgccga
     1381 aatgccgtct gaaacgtttc agacggcatt tttg
//
LOCUS       DAWKOG010000233         1364 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.233, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000233 DAWKOG010000000
VERSION     DAWKOG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.233"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(87..1010)
                     /locus_tag="WJK66_002034"
     CDS             complement(87..1010)
                     /locus_tag="WJK66_002034"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEZ7579680.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRNCG"
BASE COUNT          367 a          317 c          335 g          345 t
ORIGIN      
        1 attttaggtt tctgttttgg ttttttgtcc ttgtaggaat gatgaaattt taagttttag
       61 gaatttaccg gaaaaaacag aaagcgttat ccacaatttc tgatgttcag ctcgtgaaat
      121 gcgtcgggca aatcatcgct gtcggcaaat tccacccggt cgtaagccgt ttcgtctgcc
      181 aaaaccgcgc gcaagagtgc gttgttgatg gcgtgtcccg atttgtagcc ttcaaacgcg
      241 ccgacaatcg gatgtccgac gatatacaaa tcgccgatgg catcaaggat tttgtggcgc
      301 acaaactcat cgggatagcg caagccttca ggattcagga catccgtgtc gtcaatcacg
      361 atggcgttgt tcaaattgcc gcccaaaccc agattgtggg cgcgcatcat ttccacttcg
      421 tgcataaagc cgaaggtgcg cgcgcgcgcg atttcgtcga tgtaggattt gccggcgaaa
      481 tcaatttcaa aagtgggcga gctgcggttg aaaaccggat ggtcgaattc gatggtcagc
      541 gttaccttaa agccgtcata cggcgtaaag cgtacccatt tgcccgcttc tttgatttcg
      601 acaggcttga ggattttcaa aaaacgcttt tgcgcctttt gatcgaccac gcccgcatct
      661 tgcaaaaggt aaataaacgg caggctggag ccgtccataa tcgggatttc gggcgcgttc
      721 agctcaatca gcgcattgtc gatgccgtag gcggacagcg cggacataat gtgttcgatt
      781 gtgccgacgc gcacgccttt gtcggtaacg atggtggagg aaaggcgggt atcgttgatc
      841 aaataagggg tcagtttgat ttgttcgccc atctcgccgt ccaaatcggt acggcggaag
      901 gaaatcccgc tgttttcagg cgcggggtgc agggtcagcg cgacgcgttc gcccgaatgc
      961 agcccgactc cggtaacgct gatggatttc gccaaagttc tttgcagcat aaaccgcttc
     1021 cttatcaatg gggtaagttt tggaataata cgataaaacc ggaaaaacaa gctatgtttt
     1081 tccatagtat ttgccaatgt atctgttttc aatacgtaag ccgcataaaa atgtatagtg
     1141 gattaacaaa aatcaagaca aggcgacgaa gccgcccctc tcgaaaaacg caaaaaatgc
     1201 cgtccgaaaa cctttcggac agcattttcg cgtgaaccgt cattcccaca aggacaaaaa
     1261 accaaaacat aaaaccaaaa acagaaacct aaaattcatc attcccacga aagtgggaat
     1321 ctagttcgtt gagtttcagt tatttagaat aaattttgaa actt
//
LOCUS       DAWKOG010000234         1343 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.234, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000234 DAWKOG010000000
VERSION     DAWKOG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.234"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            58..858
                     /locus_tag="WJK66_002035"
     CDS             58..858
                     /locus_tag="WJK66_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ7579681.1"
                     /translation="MDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNN
                     PESATRYSYPAYDTTATTKSDALSSVTTSTSLLNAPAAALTRNSGRKGERSAGLSVNG
                     MGDYRNETLLANPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRSRT
                     ELHLYNAETLKAQTKLEYFAVDRDSRKLLIAPKTAAYESQYQEQYALWMGPYSVGKTV
                     KASDRLMVDFSDITPYGDTTAQNRPDFKQNNGKNPDVGNEVIRRRKGG"
     gene            862..>1343
                     /gene="mafB"
                     /locus_tag="WJK66_002036"
     CDS             862..>1343
                     /gene="mafB"
                     /locus_tag="WJK66_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ7579682.1"
                     /translation="MKPLRRLTNLLAACAVAAAAFGQPALAADLAQDPFITDNAQRQH
                     YEPGGKYHLFGDPRGSVSDRTGQINVIQDYTHQMGNLLIQQANINGTIGYHTRFSGHG
                     HEEHAPFDNHAADSASEEKGNVDDGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGA
                     R"
BASE COUNT          347 a          505 c          298 g          193 t
ORIGIN      
        1 cgctttgccg tcgaacaaga actcgtcgcc gcatcgtccc gcgccgccgt caaagaaatg
       61 gatttgtccg ccctgaaagg acgcaaagcc gccctttacg tctccgttat gggcgaccaa
      121 ggttcgggca acataagcgg cggacgctac tctatcgacg cactgatacg cggtggctac
      181 cacaacaacc ccgaaagtgc cacccgatac agctaccccg cctacgacac taccgccacc
      241 accaaatccg acgcgctctc cagcgtaacc acttccacat cgctcttaaa cgcccccgcc
      301 gccgccctga ctagaaacag cggacgcaaa ggcgaacgct ccgccggact gtccgtcaac
      361 ggcatgggcg actaccgcaa cgaaaccctg ctcgccaacc cccgcgacgt ttccttcctg
      421 accaacctca tccaaaccgt cttctacctg cgcggcatcg aagtcgtacc gcccgaatac
      481 gccgacaccg acgtattcgt aaccgtcgac gtattcggca ccgtccgcag ccgtaccgaa
      541 ctgcacctct acaacgccga aacccttaaa gcccaaacca agctcgaata tttcgccgtc
      601 gaccgcgaca gccggaaact gctgattgcc cctaaaaccg ccgcctacga atcccaatac
      661 caagaacaat acgccctctg gatgggacct tacagcgtcg gcaaaaccgt caaagcctca
      721 gaccgcctga tggtcgattt ctccgacatt accccctacg gcgacacaac cgcccaaaac
      781 cgtcccgact tcaaacaaaa caacggtaaa aaccccgatg tcggcaacga agtcatccgc
      841 cgccgcaaag gaggataaac cgtgaaaccg ctgcgcagac tgacaaacct ccttgccgcc
      901 tgcgccgtag cggccgccgc cttcggacag cccgccctcg cagcggactt ggcgcaagac
      961 ccgttcatta ccgataacgc ccaacggcag cactacgaac ccggcggcaa ataccacctc
     1021 ttcggcgacc cgcgcggcag cgtctccgac cgcaccggtc aaatcaacgt catccaagac
     1081 tatacccacc agatgggcaa cctgctcatc caacaggcaa acatcaacgg cacaatcggc
     1141 taccacaccc gcttttccgg acacggacac gaagaacacg cccccttcga caaccacgcc
     1201 gccgacagcg caagcgaaga aaaaggcaac gttgacgacg gctttaccgt ataccggctc
     1261 aactgggaag gacacgaaca tcatcccgcc gatgcctacg acggcccgaa gggcggcaat
     1321 taccccaaac ctacgggcgc acg
//
LOCUS       DAWKOG010000235         1309 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.235, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000235 DAWKOG010000000
VERSION     DAWKOG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.235"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..734)
                     /locus_tag="WJK66_002037"
     CDS             complement(<1..734)
                     /locus_tag="WJK66_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ7579683.1"
                     /translation="MLGMFSVNSYAERSKYHIGNSDVRLDIDHTKSVVTDFRVDGQRF
                     SGRIIEPSIIEHVPTGARSLEKVPVKFTASVSRAGVLAGVGKLARLGAKFSTRAVPYV
                     GTALLAHDIYEIFKEDIQEQGYQYDPETDKFVKGYEYSNCILYRVGDYPHEKTVIHSC
                     YGVDISTMRLMSDYSRFPEVKELMEARMEIIGRKYWEEFRHSPHMVNFKDYKFALCNF
                     NWNGGDCSVRKGDDYRSFITFSLERN"
     gene            complement(712..1026)
                     /locus_tag="WJK66_002038"
     CDS             complement(712..1026)
                     /locus_tag="WJK66_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ7579684.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGKTMAYTFASELLDYSKVNKF
                     LIHEAIQWFLNRRISNNIWKIYFSDESVAYIKILELQDDSSRGIEIKTFDFNTNAGDV
                     FG"
BASE COUNT          405 a          286 c          231 g          387 t
ORIGIN      
        1 tggtttcttt caagtgaaaa agtgataaaa gacctataat catctccttt acgaactgaa
       61 caatctccgc cgttccagtt gaaattacaa agggcaaatt tgtaatcttt aaaattaacc
      121 atatggggac tatgcctaaa ctcttcccaa tactttctgc caatgatttc cattctggct
      181 tccatcagtt ctttaacttc aggaaatcta ctgtaatctg acataagacg catagtcgaa
      241 atgtcgacgc catagcagga atgaattacc gtcttttcat ggggataatc tcccacccta
      301 tacaaaatgc aattactata ttcgtagcct tttacaaatt tgtcggtttc gggatcgtat
      361 tggtagcctt gttcctgaat gtcttctttg aatatttcgt aaatgtcgtg tgctaaaagg
      421 gcagtgccga cataaggaac tgcccttgtg ctgaatttcg cgcctaagcg ggcaagtttg
      481 ccgacccccg ccaatacgcc ggcgcgggaa actgatgcgg taaatttaac ggggactttt
      541 tcaagagagc gtgcgcctgt tggcacgtgt tctattattg aaggttcgat aattcgacct
      601 gaaaaacgct gaccatcaac acgaaaatcg gtaactacag attttgtatg atcaatatcc
      661 aatctaacat ctgaatttcc aatgtgatac ttggaacgtt cggcataaga attaaccgaa
      721 aacatcccca gcattagtat taaaatcaaa cgttttaatt tcaattccac gactagaatc
      781 atcctgtaat tctaaaattt ttatatacgc aacagactca tcagaaaaat aaattttcca
      841 aatattatta gaaatccttc tatttaaaaa ccattggatt gcttcatgaa ttagaaattt
      901 attaactttt gaataatcta aaagctcact agcgaaagta tatgccattg ttttaccaaa
      961 atacttgctg gacggctgat atttataaag tgccaactgc gcctgcgtga taaacggctt
     1021 gttcatggtt ctgcctttca atgattgttt tgaaagcctg attttgacac cataacttca
     1081 tacgctcaat ccttaaacag aaccgccccg attaatacgg ggactgcaat gccgagatag
     1141 aaataaaaat ccatcatttc aaaacctttt tcagcaggga aacaaagtaa acggaagcca
     1201 tcacgccgaa aaatatccag cctgtttcaa gaccgctttg caagttgtct ttcggactgc
     1261 attccgccaa tgaaagcctt agcggctgac cgtccgacat cttccacag
//
LOCUS       DAWKOG010000236         1299 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.236, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000236 DAWKOG010000000
VERSION     DAWKOG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.236"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..695)
                     /locus_tag="WJK66_002039"
     CDS             complement(<1..695)
                     /locus_tag="WJK66_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ7579685.1"
                     /translation="MYALSEKYNDNGFKAYKVLGEGGGIHTEYNYKFDKSLNLNVLES
                     STGARSLEKVPVKVTASVSRAAVLSGVGKLARLGAKLSTRAVPYVGTALLAHDVYETF
                     KEDIQAQGYQYDPETDKFVKGYEYSNCLWYEDKRRINRTYGCYGVDSSIMRLMSDDSR
                     FPEVKELMQSQMYRLARPFWNWHKEELNKLSSLDWNNFVLNRCTFDWNGGDCVVNKGD
                     DYRNGANFSLSRNP"
     gene            complement(1154..>1299)
                     /locus_tag="WJK66_002040"
     CDS             complement(1154..>1299)
                     /locus_tag="WJK66_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579686.1"
                     /translation="SDGQPLRLLLAECSPKDNLQSGLETGWIVFGILASVYFVSLLKK
                     VLK"
BASE COUNT          414 a          274 c          213 g          398 t
ORIGIN      
        1 ggattgcggc taagggaaaa attagcccca tttctgtaat catcaccttt attgaccaca
       61 caatctccgc cgttccaatc aaatgtgcaa cgatttaaaa caaaattatt ccaatccaaa
      121 gaacttaatt tattcagttc ttctttatgc caattccaaa acggacgtgc cagcctatac
      181 atttggcttt gcatcaattc tttgacttcg gggaatctgc tgtcatcgga cataaggcgc
      241 ataatcgaac tgtcaacgcc gtagcagcca taggttctat taatacgtct tttgtcttcg
      301 taccaaaggc aattactata ttcgtagcct tttacaaatt tgtcggtttc ggggtcgtat
      361 tggtagcctt gtgcctgtat gtcttctttg aaagtttcgt atacgtcatg ggctaaaagg
      421 gctgttccga cataaggaac tgcccttgtg cttaatttcg cgcctaagcg ggcaagtttg
      481 ccgactcctg acaagacggc ggcgcgggaa actgatgcag ttactttaac ggggactttt
      541 tcaagagagc gtgcgcctgt tgaactttct aatacattca aattcaaact tttatcaaat
      601 ttgtaattat attctgtatg aattcctcct ccctcaccta aaactttgta tgccttaaat
      661 ccattatcgt tatatttttc cgaaagtgca tacatcaatt tcccattttt aatttctaaa
      721 tctgctgaaa aagatttagc actcaaaaga aacagaaaag aaattattag aatccgaaac
      781 atcaattttt ccattgccaa taatgaaaaa tgaatcatcc ttgaatttaa tttcaaagca
      841 agattcatta aaattaattc tattaaaaaa attatgacat ttatccattg agaaattttt
      901 caatagacca gtttctttca aataacaata aaacacataa gaaagaggcc tatcaggata
      961 agattctgac aatttatcta aatcagaatt tgatatatag aaaaagtcac gttcattttt
     1021 attcatggtt tcaagcctca aatgtctaat taaaagcctg attttgacac cataacttca
     1081 tgcgctcaat ccttaaacag aaccgcccca attaatacgg gtacggaaac gccgagataa
     1141 aaataaaaat ccatcatttc aaaacctttt tcagcaggga aacaaagtaa acggacgcga
     1201 ggatgccgaa tactatccag cctgtttcaa gaccgctttg caggttgtct ttcggactgc
     1261 attccgccaa taaaagcctt agcggctgac cgtccgaca
//
LOCUS       DAWKOG010000237         1298 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.237, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000237 DAWKOG010000000
VERSION     DAWKOG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.237"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(126..398)
                     /locus_tag="WJK66_002041"
     CDS             complement(126..398)
                     /locus_tag="WJK66_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579687.1"
                     /translation="MFAVIFFSTLGCILAWIRDIPKIKSKKILARSLYIIGIINVIIS
                     YVLIKNILVSVSDGGGIKYVAIYLSNLFFWTVLMYVLVKRLSKKPS"
     gene            complement(960..>1298)
                     /locus_tag="WJK66_002042"
     CDS             complement(960..>1298)
                     /locus_tag="WJK66_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579688.1"
                     /translation="LSQKEMKETEGAFLPIVAGALMGGAISAWANHGISKIKTGQFAS
                     TRSTLAATAGGMIGGGYSSAMLRGAGITTSVFARSAWKGGNAIPDAVIRANGAALNQS
                     SSAAMGKHRR"
BASE COUNT          411 a          308 c          186 g          393 t
ORIGIN      
        1 ccctgcttag aacgtatgtt gcacacccaa tttcaatagg taaatcagat tgcaaatcca
       61 gcaatttgaa tattgtcatt gttccgtgca aaagggattt ttattgatga gttgtgtact
      121 gggtttcagc ttggcttttt agataatctt ttcacaagaa catacatcaa tactgtccaa
      181 aaaaaaagat ttgataaata tattgcaaca tattttattc cacctccatc cgaaacagaa
      241 accaatatat ttttaattaa tacatagctg attattacat ttattattcc tataatatat
      301 aaggatcttg ccaaaatttt ttttgatttt attttaggaa tatctcttat ccatgctaaa
      361 atacagccca atgtcgaaaa gaaaataaca gcaaacacta ccttacctcc tctcacttcc
      421 aaccaaaccg atagcaggtt tggttggaag ttggtttatt tattatttaa cggcgaatgt
      481 cagtgttctt acccgtagaa cctgcataac catttatgcc accagcaaca gtccccactg
      541 ctacacctcg cataccatta atcagaattc ttcctatttt ttcgaatcca ctatttcttc
      601 caaagatgca gcaaacgcag gttgagccta gacccaatct acgtttgctg ccgcatctga
      661 tccctattgt ttcttatcct tacatcttcc tgccttgtca atcaaataaa gacagaagac
      721 tatacaaaaa ctgaccgaca tactgaaaat acctatcccc ttccatgcaa tctccgcacc
      781 attgacccaa tagattagac tgagaaacaa caaagccaca gtataaatca acgtaaaaaa
      841 tattgcgtaa agcacaataa agggaacttt tatctcacgg ttgtttttta taatatattc
      901 agcaacttga tttccgaata tacctgataa aaaatatagt attagatcat ccatttcgtt
      961 tatcttctat gttttcccat tgcggcgcta gaagactgat ttaatgctgc accatttgca
     1021 cgaataactg catcaggaat agcatttccc cctttccaag cagaacgggc aaaaacacta
     1081 gtagtaatcc ctgcaccgcg caacatcgca ctgctatatc cgccgcctat cataccacca
     1141 gcggttgcag ccaaggtact tcttgttgaa gcaaattgcc ctgttttaat tttagaaatg
     1201 ccgtgattag cccaagcact aattgcccct cccatcaaag cacctgccac aataggaaga
     1261 aacgccccct cagtctcctt catctccttt tgagaaag
//
LOCUS       DAWKOG010000238         1295 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.238, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000238 DAWKOG010000000
VERSION     DAWKOG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.238"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            174..1232
                     /gene="alr"
                     /locus_tag="WJK66_002043"
     CDS             174..1232
                     /gene="alr"
                     /locus_tag="WJK66_002043"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEZ7579689.1"
                     /translation="MRPLNVQIRLGNLRHNYQILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLIRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
BASE COUNT          329 a          296 c          351 g          319 t
ORIGIN      
        1 ttgggaaagc gattatatca ggaaaagcaa accgccttcc tacctgaaaa ctgctgcttc
       61 ggcttgaaga cacaagggtc tttaatattt taaaagcctt gccgttggat tataattccc
      121 taattgattt cttaattttg ctaataaaca cttgtttggt aaggaatgaa tttatgcgtc
      181 ctttgaacgt gcagatcagg ttgggcaacc ttaggcacaa ttatcagatt ttaaaggaaa
      241 tgcacggagg caagctgttg gcggtagtga aggccgacgc atacggacac ggtgcggtca
      301 gatgtgcttt cgcgctggca gacttggcag acggctttgc cgtggcgaca atcgatgaag
      361 gaatcaggct gcgggagagc ggcattaccc atccgattgt ccttttggaa ggcgtatttg
      421 aagcatcaga gtacgaagcg gtcgaacaat actcgctttg gccggcagtc ggaaaccaat
      481 ggcagcttga ggctttgctg atccgccatt ggaaaaaacc cgtcaaagtc tggttgaaaa
      541 tggattcggg gatgcaccgt accggttttt tccctcatga ttacgcttcg gcatatgcgg
      601 cattgaaaca gtcggaatat gtggacagta ttgtcaaatt ctcgcatttc tcctgtgcgg
      661 acgaacccga aagcggtatg acggaaatac agatggaagc attcgatttg ggtacggaag
      721 ggctggaagg cgaagaaagc cttgccaatt cggcggctat tttgaatgtt cccgaagcac
      781 gcagggattg ggggcgcgcg ggcttggcgt tatacggcat ttccccgttc ggaggcagcg
      841 atgacaggct gaaacccgtg atgaggcttt caacccgtat ttttggcgaa cgcgttttac
      901 agccgcactc ccctatcggt tatggcgcaa cattttatac cagcaaatct acgcgcgtcg
      961 gtctgattgc ctgcggttat gcggacggtt atccgcgccg cgccccaagc aattcccccg
     1021 tcgctgtcga cggcaaattg acccgggtca tcggcagggt ctctatggat atgatgacca
     1081 tcgagctgga cgcttcgcaa gaaggtttgg gacacgaggt cgaactgtgg ggcgatacgg
     1141 tcaacatcaa tactgttgcc gaagcggccg gaaccatccc ttacgaattg atgtgcaata
     1201 tcaaacgtgc aaaattcact tatatcgagt aatcaagtcc aaacaaaaat gccgtctgaa
     1261 gcctttcaga cggcataaat gcgttttgac aaaat
//
LOCUS       DAWKOG010000239         1242 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.239, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000239 DAWKOG010000000
VERSION     DAWKOG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.239"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            15..1242
                     /locus_tag="WJK66_002044"
                     /pseudo
     CDS             15..1242
                     /locus_tag="WJK66_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          378 a          226 c          304 g          334 t
ORIGIN      
        1 taaaggatct tattatgaat gagggtgaag ttgttttaac accagaacaa atccaaacct
       61 tgcgtggtta tgctttccgt ggcgatacct atggcggttg gcgttgggtg accgttatgc
      121 ggatgatgct gctgcaattg tcggtaagga tgcaaactta aatggtttga atttatggat
      181 gaaaaaaggt gtggaaaacc tatgggatga tacggtcggt aaaaagaccc gtttagagaa
      241 atttgatcgg gttgcattgc aacatttcag ccaatatgta gatctaatta atgaaaataa
      301 tggtagatta cctaacacta gtgaaattga gagaagttac tataaagccg ttaccgaaaa
      361 tggtgtttct tctagtgcag ctattgattt agttattaat cgctcacttc cggatatggc
      421 agatggttat tgggcattag gtttggggat agaagccgaa cgtatccaca atgcgcaagc
      481 agtaaataat ccgaacggta gcgaatggga taatagaaag cagttaatat ctgctttaga
      541 taaaggattt gatggatctt ttaaagagaa gcattttact tttttacaat ctgtgataat
      601 ggatgtaaca aagttaggtg ttgaatatac aatagatggt tggcaaaaaa ttggaggttg
      661 gggtaatggg ataatcaatg atttatataa aagtgttgca aaaagagagt ggactggaat
      721 atttgagatc gttaataata acatcaagca atttagagat ctgttcccaa atccggaagg
      781 ctggatcgat gatggtcacc aatgtttcgc tccttgggtt aaagaaacta aaaaacgcaa
      841 tggcaaatat catgtctacg accccctcgc cctagatttg gacggagacg gtatagaaac
      901 cgttgccacc aaaggctttg caggcagctt atttgatcac accaacaacg gtatccgcac
      961 cgccaccggt tgggtttctg ccgatgacgg tttactcgtc cgcgatttga acggcaacgg
     1021 catcatcgac aacggtgcgg aactcttcgg cgacaacacc aaactggcag acggttcttt
     1081 tgccaaacac ggctacgcgg ctttggccga attggattca aacggcgaca acatcatcaa
     1141 cgcggcagac gccgcattcc aaaccctgcg tgtatggcag gatctcaatc aggacggcat
     1201 ttcccaagct aatgaattgc gtacccttga agaattgggt at
//
LOCUS       DAWKOG010000240         1232 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.240, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000240 DAWKOG010000000
VERSION     DAWKOG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.240"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            40..372
                     /locus_tag="WJK66_002045"
     CDS             40..372
                     /locus_tag="WJK66_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspG"
                     /protein_id="HEZ7579690.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDMPPGIPCFWVEGAESLEELCAMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GDACACGH"
     gene            704..1213
                     /locus_tag="WJK66_002046"
     CDS             704..1213
                     /locus_tag="WJK66_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEZ7579691.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGATQYGKTNSTV
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
BASE COUNT          328 a          230 c          295 g          379 t
ORIGIN      
        1 ccttgttttg acggaaaacc atcatataaa ggaacactta tgcagatttt atcttttcaa
       61 ccggacattg cggaacgtat gctggaaggt acggaaggcg agtcggtcaa cgaaaacgca
      121 caattcgtcc gtacggacaa cggctattgg attgcgtggc atgaaggcgt agcggcactg
      181 cttgcgcccg atatgccgcc gggcattccc tgtttttggg tggaaggcgc ggaaagcctt
      241 gaagagttgt gcgccatggt ggaacgcggc gagtttgacg aagtggaaga gtttgacggc
      301 gatgacgacg aatggctcga aacggcacag ggttgcgggc accacggcga cgcttgcgcc
      361 tgcggacatt aaaggcattg caggcttgcc gcaaggggtg caaggctttg ccgtttttta
      421 aataatcggt ggattgcatc tgtttgcaat gggcggaacg cttccctttg tattttattg
      481 aataaaaaaa cttatcttgg tattataatt aaggtagcat caattatttt gggatggcaa
      541 taaacgcaaa gcattgattt gcgccgattg cagacttatt atagcaggtt gcggcgcgga
      601 cttaatggga attgtttata gagcatgatg gtctgaaaca tcattaatta aaaataccat
      661 taatccgatt tatatttatt tcaatttcaa tggaaaaaca tcaatgacaa tgattttaag
      721 cattttaagc ctgtttttta tcatcagact gttattttta gccgtctcta ttaaacatga
      781 aaaagccttg attgccaaag gggcaacgca atacggaaaa accaattcca cagtgcttgc
      841 ggcagttcat acgctttatt atttggcgtg ttttgtttgg gtatggcttt ctgacactgc
      901 ttttaatggc atatccttga ttggtacgct gacggtgatg gcttcgtttg tgatattgtc
      961 attgattatt aagcagttgg gggagatttg gacggttaaa atctatattt taccaaatca
     1021 tcaaattaat cgttcgtggt tgtttaaaac attccgccac cccaattatt ttttaaacat
     1081 catacccgaa ctgattggca tcgccttatt atgtcaagcg tggtatgttt tattgattgg
     1141 cctgccgatt tatttgctgg tcttatttaa gcgtatccga caagaagaac aggcgatggc
     1201 aacacttttt taacccgttt catcaattat ag
//
LOCUS       DAWKOG010000241         1185 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.241, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000241 DAWKOG010000000
VERSION     DAWKOG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.241"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..456)
                     /locus_tag="WJK66_002047"
     CDS             complement(<1..456)
                     /locus_tag="WJK66_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579692.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     LYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNIQTLD"
BASE COUNT          427 a          216 c          121 g          421 t
ORIGIN      
        1 gtctagagtc tgtatattac atttattttt agggtctgct agccaatttc ttgttccctt
       61 cattatttta tcttctgaaa gaaaattatt tttttccatg ctattaatat taatgatatg
      121 atttttattt aaaataattt ttccatataa aatttccggt ttgtaatagc aagagtctga
      181 agcaaatcct tgcccgtcaa tataagaaaa ataaatgtta tcatcctcct tataatattc
      241 taaaaccaat ccggatatta ttttatcaaa attcccttca tttccatctt tcatgactaa
      301 aatataatta gcaggccaac ctttatcgta aacatattca aactgatagt tcccgaaact
      361 ctcgatatcc gaactaaaaa agaagaaaag caaattaata gccaatacaa aaacaaataa
      421 gaacaaaaat agcaaaattt tcaacttagt taacaatatt tacctctcct ttaaattcaa
      481 tcctgaaagg taccccttac cctgggctac caattatagt ttccatattt ctaaatattg
      541 tttttacatt acttttttct ccccccaaag gaattcattt taaaatcatg cttttcaggt
      601 gctaatcgat acttaccatt aacatcttta atcacagata tatttccatg tatagcccaa
      661 cgtgaaaaat ctgagtatta tatacagtta taccttactg aaacaaagct agctgtacat
      721 atctgtacca tatttttact acaaacaaaa aatatttata attattaagg ataagataaa
      781 aacccccaac gccagattaa ttcttaataa tttcaaataa aaaatatttg agattttttc
      841 ttttccaaat aatatattga aatatttaaa gttaaggtag cttgatttaa gtagaaaatc
      901 aaaaaaacca ttaattaatt tttcttcttt aaatatttta tatgatatta taaaattaaa
      961 taacattgcg aaaacatatg aaatcatcaa aataccaaat atatagtgag aatgcattat
     1021 tatttagctc cttcttttgg ctctgacaaa ttgacagtcc attgatatcc aatgcttgca
     1081 tttgcaattg ctgacgcacc tgcccctgtt ttccctgtta gtatcaatgg ttcttttctt
     1141 gcaagatgtg cacctgcaca accacctaat actaaaccac cacct
//
LOCUS       DAWKOG010000242         1144 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.242, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000242 DAWKOG010000000
VERSION     DAWKOG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.242"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            32..>1144
                     /gene="tuf"
                     /locus_tag="WJK66_002048"
     CDS             32..>1144
                     /gene="tuf"
                     /locus_tag="WJK66_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ7579693.1"
                     /translation="MGHVDHGKTTLTAALTTILAKKFGGAAKAYDQIDNAPEEKARGI
                     TINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREH
                     ILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSSYDFPGDDCPIVQGSALK
                     ALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDVFSISGRGTVVTGRVERG
                     IIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQVL
                     AKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFRTTDVTGAVTLEEGVEMV
                     MPGENVTITVELIAPIAMEEGLRFAIREGGRTVGAGV"
BASE COUNT          302 a          313 c          295 g          234 t
ORIGIN      
        1 acgtagcaaa ccgcacgtaa acgttggcac catcggtcac gttgaccatg gtaaaaccac
       61 cctgactgcc gctttgacta ctattttggc taaaaaattc ggcggtgctg caaaagctta
      121 cgaccaaatc gacaacgcac ccgaagaaaa agcacgcggt attaccatta acacctcgca
      181 cgtggaatac gaaaccgaaa cccgccacta cgcacacgta gactgtccgg ggcacgccga
      241 ctacgttaaa aacatgatta ccggcgccgc acaaatggac ggtgcaatcc tggtatgttc
      301 cgcagccgac ggtcctatgc cgcaaacccg cgaacacatc ctgctggccc gtcaagtagg
      361 cgtaccttac atcatcgtgt tcatgaacaa atgcgacatg gtcgacgatg ccgagctgtt
      421 ggaactggtt gaaatggaaa tccgcgacct gctgtccagc tacgacttcc ccggcgacga
      481 ctgcccgatc gtacaaggtt ccgcactgaa agccttggaa ggcgatgccg cttacgaaga
      541 aaaaatcttc gaattggctg ctgcattgga cagctacatc ccgactcccg agcgtgccgt
      601 ggacaaacct ttcttgttgc ctatcgaaga cgtattctct atttccggtc gtggtacagt
      661 agtaaccggt cgtgtagagc gcggtatcat ccacgtcggt gacgagatcg aaatcgtcgg
      721 tctgaaagaa actcaaaaaa ccacttgtac cggtgttgaa atgttccgca aactgctgga
      781 cgaaggtcaa gcaggcgaca acgtaggcgt attgctgcgc ggtaccaaac gtgaagacgt
      841 agagcgtggt caagtattgg ctaaaccggg tacaatcact cctcacacca agttcaaagc
      901 agaagtatac gtactgagca aagaagaggg cggccgccat actccgttct tcgccaacta
      961 ccgtccgcaa ttctacttcc gtaccaccga cgtaaccggc gcggttactt tggaagaagg
     1021 tgtggaaatg gtaatgccgg gcgagaacgt aaccatcacc gtagaactga ttgcgcctat
     1081 cgctatggaa gaaggtttgc gctttgcgat tcgcgaaggc ggccgtaccg tgggtgccgg
     1141 cgtg
//
LOCUS       DAWKOG010000243         1126 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.243, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000243 DAWKOG010000000
VERSION     DAWKOG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.243"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(229..1083)
                     /locus_tag="WJK66_002049"
     CDS             complement(229..1083)
                     /locus_tag="WJK66_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ7579694.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGVGCSW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSVLGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT          312 a          336 c          295 g          183 t
ORIGIN      
        1 ccgtcattcc cgcgcaggcg ggaatccgga catccaacgc tgcagcaatt tgtcggaaat
       61 aaccgaaact caaaaaaccg gatgcccgcc tgcgcgggaa tgacgatgca aagatggtcg
      121 ttatttcgga taaatatccg tcttttcgtt ccggataccc gctttcgcag gaatggagat
      181 ggaaagattt cagcctattc ggcaatcatc aaataccgct tcccaacttt atttttgatg
      241 cgacagccgg ccggcaacca aggtggcggc gatgacgaca aacacgccca agacggacac
      301 gggcgacagg tgttcgccca aaatcaaaac cgccagcagc acgccgacga cgggttcgag
      361 cgaaatcaac agtcccgaaa cgttggcagg aacacggctc atccccttgt tccacagcca
      421 ataggcgtac cagctgcacc ccacgcccaa atacagcagc gacaatacca ttccgacgct
      481 ccagtccacg gtataacttt gcgccaaagc aagcgaaaac ggcaggcaca tcaacgatgc
      541 ggcggcaatg gaaacagatg tgaatgccgg tgcgccgatg cgtgcaatca gcctttgcgt
      601 cggacgcata gcggcacaaa agcccgcgcc cgccaacaac accagcaggc agccgaacca
      661 gccgacttcg ccgccctctt ccgcaccgcc cgccatcagc agcgcgacac cggcaaatgc
      721 cgccgcgccg catatccagt ggtaggcacg cgctttgtcg ttgaagaaaa agtgtccgac
      781 aaacaccatc agcagtggct cgagtccgac aatgaccgat gcgctggcgg cggaagtgta
      841 tttcaacccg acaaactgaa gcagcagggt cagcacatag ttgacgaacg acacaatcag
      901 caacggcttc cattcctcac gcggaatctt gccgacatga cggcggcagg cgggcagtgc
      961 aggcagcgca gcaatcagca ggcgcacgcc gaccatcaat gcgggatcga tgccgccata
     1021 gacatatttg gcggcaataa acgagctgct ccagataatc agggcaagga tttggtaaaa
     1081 catagtgagt gataaagtca gatggtcata caaaagtata gcggat
//
LOCUS       DAWKOG010000244         1126 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.244, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000244 DAWKOG010000000
VERSION     DAWKOG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.244"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            81..1046
                     /locus_tag="WJK66_002050"
     CDS             81..1046
                     /locus_tag="WJK66_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7579695.1"
                     /translation="MLRLTALAVCTALALGACSPQNSDSAPQAKEQAVSAAQSESASL
                     TVKTAHGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSVDKNRLPYLEEYFKTTKPA
                     GTLFEPDYETLNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWL
                     HKDIGLPAVDEAIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLDNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
BASE COUNT          288 a          316 c          296 g          226 t
ORIGIN      
        1 ttatcattta tcctttctaa agccgttccg gtttgtccga ccggcggctt tgccccaata
       61 tccccatttt ggagacacct atgttacgtt tgactgcttt agccgtatgc accgccctcg
      121 ctttgggcgc gtgttcgccg caaaattccg actctgcccc acaagccaaa gaacaggcgg
      181 tttccgccgc acaatccgaa agtgcgtccc tgaccgtcaa aacggcgcac ggcgatgttc
      241 aaataccgca aaaccccgaa cgtatcgccg tttacgattt gggtatgctc gacaccttga
      301 gcaaactggg cgtgaaaacc ggtttgtccg tcgataaaaa ccgcctgccg tatttagagg
      361 aatatttcaa aacgaccaaa cccgccggaa ctttgttcga gccggattac gaaaccttaa
      421 atgcctacaa gccgcagctc atcatcatcg gcagccgcgc cgccaaggcg tttgacaaat
      481 taaacgaaat cgcgccgacc atcgaaatga ccgccgatac cgccaacctc aaagaaagtg
      541 ccaaagagcg tatcgacgcg ctggcgcaaa tcttcggcaa acaggcggaa gccgacaagc
      601 tgaaggcgga aatcgatgct tcgtttgaag cggcgaaaac tgccgcacaa ggcaaaggca
      661 aaggtttggt gattttggtc aacggcggca agatgtccgc cttcggcccg tcttcacgac
      721 tgggcggctg gctgcacaaa gacatcggtt tgcccgctgt tgacgaagcc atcaaagaag
      781 gcagccacgg tcagcctatc agctttgaat acctgaaaga gaaaaatccc gactggctgt
      841 ttgtcctcga ccgaagcgcg gccatcggcg aagagggtca ggcggcgaaa gacgtgttgg
      901 ataatccgct ggttgccgaa acaaccgctt ggaaaaaagg acaggtggtt taccttgttc
      961 ctgaaactta tctggcggcc ggcggcgcgc aagagctgct gaatgcaagc aaacaggttg
     1021 cggatgcgtt caacgccgcg aaataatgaa acggcggcat tcgatgccgt ctgaaacacg
     1081 gatgcaaacc gcttcccgtg tttcagacgg cattgcccga tacggg
//
LOCUS       DAWKOG010000245         1112 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.245, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000245 DAWKOG010000000
VERSION     DAWKOG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.245"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            153..1052
                     /locus_tag="WJK66_002051"
     CDS             153..1052
                     /locus_tag="WJK66_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ7579696.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFGQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFGERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFA
                     SGLVSGLMLALSMVFVRKTHEIEPVALFPSMMILSLGGAVSLVVPALLMDGGVLYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQS"
BASE COUNT          187 a          256 c          355 g          314 t
ORIGIN      
        1 gggttttatc cggcaggacg gttttttgcc ccaacggaaa atagcctgcc tgcccgtaaa
       61 atcagccgtt tgtccgggtg cagccggggc tttgggcttc agacggcata ttttcggaat
      121 ggcggcattc ttgccgtcgg cgcggcagcc gtatggggaa gggaggggat attgtggtcg
      181 gtaacggcaa aaaatatgcc gcaccattgc tggtgctggg ttgcgtggtg ttcggtctgg
      241 gcagtctgat tgtcagatcc gtccccgtcg gttcgtatgc aatcgcattt tggcggttgc
      301 tgatttcggt gttcgtattt tggtttttag cacggttttt cgggcaaaaa ttcccaaaaa
      361 acaggaaaac cgtccgatat gccctgacgg cgggcgtgtt tctcgctttc gatttggcgt
      421 tgtggcacga aagcatacac gcggtcgggc cgggtatttc caccctgctc aacagcctgc
      481 aaatcttttt cttgtcggca atcggtgttt tctttttcgg cgagcgtttg agcgggctga
      541 aaaaggcagg cttaatatcg gcagttgccg gcgtggcgat gattgccggt gcggaattcg
      601 gctacaacgg taatgcggtt tggggattcg ccagcggttt ggtatcggga ctgatgctcg
      661 ccctgtcgat ggtgttcgtc cgcaaaaccc atgaaatcga gccggtggcg cttttccctt
      721 caatgatgat tttgagtttg ggcggcgcgg tatcgctggt tgttccggca ttgctgatgg
      781 atggcggcgt gctttatccg acgacttgga aagatgcggg tttggtgctt gtgtacggcg
      841 tggtgatgca gtgcttcgcg tgggcgatgg ttgcctatgc gattccgctg ctttcgctgt
      901 cgctgacggg gctgctgctt ttgtccgaac cggttgccgc cctgttcatc gattatttcg
      961 ggttgggcaa accgattgaa ggcgtgcagt gggcaggggt ggcgctgacg ctttcggcaa
     1021 tttacctcgg ttcgctgaaa cagcagtctt aatattgatt ccatcaaggc agtattggca
     1081 gttttccgtg caaaacctgc gttaccggac tg
//
LOCUS       DAWKOG010000246         1090 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.246, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000246 DAWKOG010000000
VERSION     DAWKOG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.246"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(350..991)
                     /locus_tag="WJK66_002052"
     CDS             complement(350..991)
                     /locus_tag="WJK66_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HEZ7579697.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIEWPQKVMGYLMMMSEKSGVPLYFDRAL"
BASE COUNT          312 a          282 c          198 g          298 t
ORIGIN      
        1 caaagaaatc cattaaacta tctttcacat tagaaattta aaagctaaat aatacaacaa
       61 acaatgtgaa gtactattca tggtttattt aaaaaataat actattctga acattattta
      121 gatacagaaa ttaacaaatt agaactaaac aagcttttaa atactttaat tttattggaa
      181 agctataaaa ggaactataa ctttacacac tagtcacttc tttttaagag gcaaaaggga
      241 ttgggaaggt cgtcttggag ataagcactg gtatttcggc caatggtaaa tagagtttac
      301 ctcaaatagg gtagaacctc cttcatctgt cagttaataa cagccacttt tacaatgccc
      361 tgtcaaaata aagcggcacg cccgattttt cactcatcat catcaaataa cccatcacct
      421 tttgaggcca ttcgataccg cgcaccacgg tcagattcct caaaacgggg aaaaccaaaa
      481 tatcctccat gccgattccg ccgttgatgc cgtctgaagc accgtccatc aaattctcca
      541 actcttgcaa atctgcgttt atgcgttcga gatattgggc ggttttattc aaattggcgg
      601 aaaagctgcc gatgcttttc tcttttttgt ctgtaaaata ttttaccgct tccggcgttg
      661 caaattcggg cagcccgatt ttgatcacgc gcggctgcac cagtttgtcg ttgtatccgc
      721 ccaccttgtc cagccacgcc cgtatctcgg ggcggacttc gtctttcaga cggtcttcgc
      781 ggtcgaaatg ccgcacaatg tccaaactct cgcccataaa cgaaccatct tctttttgca
      841 ggacgggcac ttgtttcgca ccgatcatac cgatcggcgt tgcctcgtcg tcgtttgcca
      901 gcacggcttc ttcaacgtcc gcgccaaaca gcccggcagc catccgcgca cgcacacaaa
      961 acgggcaatg gtcgtaaata tacagtttca tcaaaatatt cctcgtcaac ctgtcggtac
     1021 cggctacctt aacaccccgc gccacccgaa acaagtttat cttcccgcct atgcaccgta
     1081 aataaataag
//
LOCUS       DAWKOG010000247         1048 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.247, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000247 DAWKOG010000000
VERSION     DAWKOG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.247"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(122..550)
                     /locus_tag="WJK66_002053"
     CDS             complement(122..550)
                     /locus_tag="WJK66_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HEZ7579698.1"
                     /translation="MGFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KKSGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
     gene            complement(531..965)
                     /locus_tag="WJK66_002054"
     CDS             complement(531..965)
                     /locus_tag="WJK66_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HEZ7579699.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTDKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLRRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKAGGDDGVRKS"
BASE COUNT          267 a          314 c          187 g          280 t
ORIGIN      
        1 tttgaaaacc ccattaaacc gtctttaacc catcgtcccg aacggtagat gtttcacccc
       61 tgcaaaccaa aaaaaagacc gtctgaatac agacggccaa agcctggtca cacatcactg
      121 cctaaaataa agctgcctgc tgtgccgcag gtcggctcat ttcattgata atcgtatatc
      181 ccgttcgtga agagatgccg tatttagggc atagcttcgt catcgccata agcccgctct
      241 tcttatcaat atcgcgcaat ttgacaaact cctgataaaa cctatggttt ctcaactgta
      301 ttaacgcctt gccgcaccgt gggacataca attcctcgcc accatatacc tgcaacagct
      361 catgtgtttt cacttcgccg atggcttcga ccaaaattgc caaacgctcg gtgtccacct
      421 tgcccttacc aaatttaaac cgcgccccgc caatcgcctt gaccagctgt tccgtcgctg
      481 ccagtccgat gacatccaca atgtccaaca cggtatccgg caataaatgt tcaacttttt
      541 cgaaccccat catccccacc cgctttttcc gttttcctgt tttccgcaat ctgcaacgca
      601 gcaaccagtt tatgtagctg cgtatcgtct aaatattcga ccttatcctt accaaacatc
      661 cgccgcgcca ttgcgtgtgc atagttccaa tgtttgccgc cgacggtcag cagggcttcg
      721 actttgtcca gcattgccgc tgatgatgtc cgacgcagat gcggtttgcc gtgtgggtta
      781 ccttttgctt taggcttaaa tccgtgcgac cgcatatcag cgacaacaga ctcaagttca
      841 gaaacatcca tatccgcaca cgaccgctta tccgtcacac gctccaacac cgcgcgatag
      901 gtaccgtcat ccaagcccag ctccttttga gcaatcttaa ttttcgcaat caacgcccgg
      961 cgcattatct ctccaataca acatatagta taaattagcg cattttatac aataaaatac
     1021 aatatatagt attaagccga tgtttttt
//
LOCUS       DAWKOG010000248         1028 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.248, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000248 DAWKOG010000000
VERSION     DAWKOG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.248"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(247..972)
                     /locus_tag="WJK66_002055"
     CDS             complement(247..972)
                     /locus_tag="WJK66_002055"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEZ7579700.1"
                     /translation="MPRFHLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHAARLSFLEKRRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLGDIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAAIAAMQTLWGDFA"
BASE COUNT          175 a          226 c          349 g          278 t
ORIGIN      
        1 tgcgcgggaa tgacggcgga gagggtttgt tgtttcggat aaatccctgt agtttttcgt
       61 ttctagattc ccgcctgcgc gggaatgacg gcggagaggg ttgttgtttc ggataaatcc
      121 ctgtagtttt tcgtttctag attcccgctt tcgcgggaat gacgacacgg gaaatcttat
      181 ttgaacctac tatgtcttaa tcggctgcaa aatgccgtct gaacgggttt cagacggtat
      241 ttctgtttat gcgaaatcgc cccaaagtgt ctgcatcgcg gcgatggcgg cgagtggggc
      301 ggtttctgtg cgtaaaatcc gtttgccgag tgtaaccgcc tgaaagccgg cttcaaatgc
      361 ctgttgttct tcctgttctg tccagccgcc ttcgggcccg accataaaga cgattgcgcc
      421 ggacgggtgg cggatgtcgc cgagtttgcg ggcgcggttg atgctcataa tcagcttggt
      481 gctttcagac ggcattttgt cgagtgcttc acgatagccg atgatgggca gtacgggggg
      541 aacggtgttt ctgccgcttt gttcgcacgc ggagatgacg atttcctgcc agcgtgcgag
      601 gcgtttggcg gcgcgttccc cgtcgaggcg gacgatgcag cgttcgctga tgacgggctg
      661 tatggcggtt acgccgagtt cgacgctttt ttgcagggtg aaatccatgc gatcgccgga
      721 ggagatggat tgtatcagtg taatgttgag cggggattcg ttgtcggttg tgtcttcgtg
      781 caggatttcg gcttcggcgc ggcgtttttc caaaaaagac agccgtgcgg cgtgtgcctt
      841 gcctttgccg tcgaagaggg tgatgttttc gttggggcgg acgcgcagga cgttgaggtg
      901 gcggacgatg ttgtcgggca aggcgacggt ttgtccgacg gaaaggtttt cgggcaggtg
      961 gaatcggggc atagtttttg gcggttcgga cggttataat ctgtcgcagc ggcgtgatgg
     1021 aaacggac
//
LOCUS       DAWKOG010000249          958 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.249, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000249 DAWKOG010000000
VERSION     DAWKOG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.249"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..288
                     /locus_tag="WJK66_002056"
     CDS             <1..288
                     /locus_tag="WJK66_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEZ7579701.1"
                     /translation="SPTGLGWMHWVVADIPADVRRRNAASLQLSRCANIADDQSAAIS
                     AVISLRICRIRLTPSYTAKPMPSCCNHANTPQSAASAALCGTSSSVSTAAA"
     gene            complement(377..656)
                     /locus_tag="WJK66_002057"
                     /pseudo
     CDS             complement(377..656)
                     /locus_tag="WJK66_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
BASE COUNT          214 a          320 c          233 g          191 t
ORIGIN      
        1 tcgccgaccg gactgggctg gatgcactgg gtggtcgccg acattcccgc cgatgtccgc
       61 cgccgcaacg cggcctcgct gcaattaagc cgctgcgcca acatcgccga cgaccagtcc
      121 gcagccatat cggcggtaat cagtttgcgg atttgccgca tcaggttgac gccttcgtac
      181 acggcaaaac cgatgccgtc atgctgcaac cacgccaaca cgccgcaaag cgcggcctcc
      241 gccgcattgt gcggcacttc ttcatccgtc agcaccgccg ccgcataatc aaacgccgcg
      301 cccatccgcc cggggtgcgg cagcttcacc gcatcgcaca ctgcctgcgc cgtcccgtat
      361 tgtgcggcga acctttctac ggtttcctgt tcaaaagcaa tccattgcgc ctgatagagg
      421 ccgtctgaat cgggaatatt aataacgtca aacgtctgtc cgcctgccaa cgcaaccgcc
      481 tcacccgccg caatcctcaa ttcgcgcctc gcccaccgca gcttcttgct tccgcgccgc
      541 acgataagca cagctggttc atataccgcc acgctgcggt acaaggcggt atgatgttgc
      601 acgatgccgc ccaaagcacc caatcgttcg cgcgtatgaa agtaatttga cggcactatt
      661 atctccaaca cgcagggccg cacataaggc gcagccctgt ttaccctgat ttggaagggg
      721 ttacttccca aaaataaacc aaaatcccac tgccccgaat ggccgggttt caaccatatt
      781 ccgtcggtgt gcctgcaact tgactccctc ccctgtgggg gagggttggg gagagggtaa
      841 aacggggcag atacagacaa tatttccgtt accgccccga tgccctctcc ctatccctct
      901 cccacgaaag agggaatgga ttgccgttta aataaaaaaa tctacataaa aatcaatg
//
LOCUS       DAWKOG010000250          953 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.250, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000250 DAWKOG010000000
VERSION     DAWKOG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.250"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(34..225)
                     /locus_tag="WJK66_002058"
     CDS             complement(34..225)
                     /locus_tag="WJK66_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579702.1"
                     /translation="MDFQFFVETIRRQEKKLKHGVYRLFEIYSWLEDGKATKARIRYF
                     VKIEVDKQILRRIHRGIAD"
     gene            354..914
                     /locus_tag="WJK66_002059"
     CDS             354..914
                     /locus_tag="WJK66_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit pseudouridine synthase E"
                     /protein_id="HEZ7579703.1"
                     /translation="MKNLIAFNKPYGVICQFSPHEKHKSLKDFINFPGFYPAGRLDTD
                     SEGLLLLTDDGRLQAQITDPKFKHPKTYWAQLEGVPDESRLESLRKGIDLGGFVTRPA
                     SIRILKHGEADSLWERIPPIRVRKTVPDFWIEITISEGKNRQVRRMTAKAGYPCLRLI
                     RVASGRLKLFDLDLKPGEWAYAPFKP"
BASE COUNT          267 a          269 c          192 g          225 t
ORIGIN      
        1 agccaaggcg agacaacgac gtaccggttt ttgttaatcc gctataccac gatgaatcct
       61 tcgcaatatc tgtttatcga cctcaatttt gacaaaatac cggatacgcg cctttgttgc
      121 ttttccatct tccaaccaac tgtaaatctc aaacagccgg tacacgccat gcttcagttt
      181 cttttcctgt cggcggattg tttcgacaaa gaattgaaaa tccatttcat gcaccttaaa
      241 atttaatctg cattcaaacc ttttcacttt ggaagcacca tttatcggat gtcccttcgc
      301 aataaacaaa ttttcccgat accgccgccc atttcaaccc aaacccaaaa cctatgaaaa
      361 acctcatcgc cttcaacaaa ccctatggcg ttatctgcca attttcaccg cacgaaaaac
      421 acaaaagcct caaagacttt atcaattttc ccggcttcta ccccgccgga cggctcgaca
      481 ccgacagcga ggggctgctg ctgctgaccg acgacggcag gcttcaggca caaattaccg
      541 accccaaatt caaacaccct aaaacctact gggcgcaact ggagggcgta cccgacgaaa
      601 gccgattgga aagcctaaga aaagggatag acttaggcgg tttcgttacc cgtccggcaa
      661 gcatccgcat cttgaaacac ggagaagcag attcgttatg ggagcgcatc ccgccgatac
      721 gcgtccgcaa aaccgttccc gatttttgga ttgaaattac catttctgag ggcaaaaacc
      781 gccaagtcag gcgaatgacc gccaaggcgg gctatccctg cctgcgtctg atcagagtgg
      841 caagcggcag gctgaaactg tttgatttgg atttaaaacc cggggaatgg gcatacgccc
      901 cgtttaaacc ataatcacgt ttatctcatc atttccacaa aagtgggaat ccg
//
LOCUS       DAWKOG010000251          950 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.251, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000251 DAWKOG010000000
VERSION     DAWKOG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.251"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            220..891
                     /locus_tag="WJK66_002060"
     CDS             220..891
                     /locus_tag="WJK66_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ7579704.1"
                     /translation="MFPDKYKLSLEENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVDISLLKKINNIVAKDDSLAPG
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGID"
BASE COUNT          273 a          214 c          211 g          252 t
ORIGIN      
        1 acggatgaaa tcaaccgata ttcaaacaca gtcattttta gcgcattttc agcgtatcgt
       61 taatgcggaa aatttcgtga acaggttttt tgcacaggtc tccgcctcgt ttcgcgggat
      121 gggaaaccgt attagaaaac ggacgcacgc aacataaacc ccgaaagtga tgataagatg
      181 atgatttaac gtactgcttt aattatttaa ggaattatcg tgtttcccga caaatacaag
      241 ttgagtttgg aagaaaatat ttttctggca aagaaagtat tggttgccca aattcacaac
      301 ctcagccgtt ttgagaattg tcagacgacc ttgttgcaga ccgaacaaat tatccatggc
      361 aaaaatgtag cctccgcgtc actggaagac atccaaacca tcttgaacct gaaacgtgcc
      421 tatcaatatg tgatttcgca tatttcaaac ggcgaaccgg tcgatatttc actccttaaa
      481 aaaatcaaca acattgttgc caaggacgat tctttggcac ccggtgattt ccgtaccggt
      541 tcggtcggcg taacgctatt ggacggttcc cgtcatgccc caaatccggt gaaggaaatt
      601 gaagtggctc gcgtgttaca aaatatcgga ctgcaaagcg gttcgacgac ggaggcagcc
      661 gtccgtttta tgctttattg tatgcggcag caggtttttt gggacggcaa caaacgaacg
      721 gcaaccttat ttgccaacgg tctgatgatg gcggggggct gcggcatctt ggaaatctcc
      781 gaaatgcaga tgccgcaatt caatgaaaaa ctgtccgcat tctatcgctc cggcgacgat
      841 accgatattt ccaagtttgt gtatcaaaat tgtatatcgg gcatagactg agacctttgc
      901 aaaattcccc aaaatcccct aaattcccac caagacattt aggggatttc
//
LOCUS       DAWKOG010000252          902 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.252, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000252 DAWKOG010000000
VERSION     DAWKOG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.252"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(85..717)
                     /gene="mtrR"
                     /locus_tag="WJK66_002061"
     CDS             complement(85..717)
                     /gene="mtrR"
                     /locus_tag="WJK66_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HEZ7579705.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAEDAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLENHPDLRRK"
BASE COUNT          200 a          201 c          237 g          264 t
ORIGIN      
        1 tatttttaga agaaggcttt gtgtcatttt gatgccgtct gaaagggttt gttcgtttca
       61 gacggcattg ttaccaaggc ttgattattt ccggcgcagg tcgggatggt tttccaagtt
      121 gtccatcatt atcccgatga tgcgcggggc ggttttgccc aaatcgaaac gttcgcagga
      181 agagaaccac cgccaaatca gcccgtccaa ggttgatttg atgaagatga ccgctgtttc
      241 cttgtccaaa tcgtcagcca aatcctgatt ttccaccgct tcggtcaaaa cggcggtaat
      301 tttctcgcgc cagattgcct gatgcttgcg ggcaatggcg ataacggcgg cgttttgctc
      361 cgtgtgttcg cattttaaaa acaggatgtt gtggaatttg tagtagatgt cgttgctttg
      421 cagccgctcg aaaaagtgca gcagcgtgtg gcggaatacc gcccaagacc ctccttcggc
      481 atcttcggca tcttgcgcga tgcagttttc gatgtcgtcg cagatacgtt ggaacagcgc
      541 gtcgaacaag tcttccttat ttttgaaatg ccaatagagc gcgccgcgcg ttacgccggc
      601 ggcttgggcg atttcgttga gcgaggtgcg cgcaatccct ttgcggtaaa aggtttccaa
      661 ggcggcaagc atcaggtgtt ctttggtttt caaggcttcg gttttggttt ttctcataat
      721 ggcgttttcg tttcgggtcg gtttgatgag ggcggattat aaaaaagact ttgtaaccat
      781 gcaatcgtgt atgtataatg aaacccatga aattgagact acatctcaac tttgaaaaca
      841 ggcataacct gctttgcaac ccgttcgaac attcgggtat caaagcaggc ggtttttata
      901 tc
//
LOCUS       DAWKOG010000253          874 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.253, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000253 DAWKOG010000000
VERSION     DAWKOG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.253"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(55..834)
                     /locus_tag="WJK66_002062"
     CDS             complement(55..834)
                     /locus_tag="WJK66_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEZ7579706.1"
                     /translation="MKLVRPQKISDQVLSVLEGRIVEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSIMKDREYQSRNRHADTLAQNDLKRVQDWEV"
BASE COUNT          203 a          288 c          174 g          209 t
ORIGIN      
        1 aatttagttc actataccga tacatccgaa tatgccagac cgtctaaacg gtattcatac
       61 ctcccaatcc tgcacgcgct tcaaatcgtt ttgcgccaaa gtatccgcgt ggcggttacg
      121 gctctgatat tccctgtctt tcatgatgct gcttgccaca taattcaaat gtgcctttgc
      181 cgcctccgaa gcctcgcccg gccgacggtt cgatatcgcc tcatacagca cgcggtgctg
      241 cgccatcagt ttcggacgcg gatcttcttc ctgattcaga taaataaggc tgctgcgcgt
      301 ctgccggtac agcattttca acaaaccgcc cgacaaatgg ctgaacaaca aattgtgcgc
      361 cgcatccgca atcgtctgat ggaagctgac atcggcttcg ctctgatgtt ccagattgcc
      421 gctttcgcac gcctcctcaa acttttcaag ccaaaaccca atccgcttca aatcggcatc
      481 cgtccggcgt tctgccgcca atgccgccat acagccctcg atgtggcagc tgaaatcaaa
      541 aacatcctgt tcccaattgg aatgcttgcc caaaagctcc tgccaacttt gcaaaaaatc
      601 ctgctgcggc ttgaccgaaa cataataacc gtctccctgc ctcgcttcca aaacctgacg
      661 ggcgaccaaa acattcaatg ccgacctgac cgacgggcgg gacacgccga actcttccgc
      721 caaaacgcgt tcgggcggaa tcttgccccc ttccgcgtaa accccttcca cgatgcggcc
      781 ttccaatacc gacaatacct gatcgctgat tttctgaggc cttaccagtt tcatcactcc
      841 tcctttataa agattccctg ccgaaccctt ccga
//
LOCUS       DAWKOG010000254          829 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.254, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000254 DAWKOG010000000
VERSION     DAWKOG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.254"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
BASE COUNT          198 a          204 c          220 g          207 t
ORIGIN      
        1 agggctaatc tagtacagcc ccttagattt aattcactat acctgcgtaa tggtatcggg
       61 tactcatcat caacctgcct tgcattcggt tgtgttgtcc ggcaactgtc cggttgtgtc
      121 gatgggttgc cctgccgctc cctgtgctgc gcaacggttg tgtgccttgg gtgggcgcgg
      181 gtggcggccg ggcggtttgt tgctatgata cgggagttcc gatatacaag ggtgggcttc
      241 agcccaccaa tcaacgaccg aaaaagaaaa aacgttgggc tgaagcccac tctacgcaat
      301 tacgcaactg ttatcgtggg gatgacggga ttttaggttt ttgtttttct gtttttgcgg
      361 gaaggatggg ttttaggttg cggtcattta tcggaaaagc agaaatcgct ccgccgtcat
      421 tcccgcgaaa gcgggaatct gaacacgtcc gtagggaaac ctatatcccg tcattcccac
      481 gaaagtggga atctagaacc tctgaatttt cagacaacct ttgaatattg ccgccgtccc
      541 gcgttctaga ttcccgcctg cgcgggaatg acgattcatc agtttcccga aacgcccgaa
      601 aaaccgaaac cgagcaaacc ggattcccgc ctgcgcggga atgacgattt ataagtttcc
      661 cgaaacgccc aaaaaaaccg aaatccgaca ggcgggattt ttgctttcgt ggggatgacg
      721 ggattttaag ttacggtcat ttatcggaaa agtagaaatc gctccgccgt cattcccgcg
      781 aaagcgggaa tctgaacacg tccgcacgga aacctatagt ggattaaca
//
LOCUS       DAWKOG010000255          752 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.255, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000255 DAWKOG010000000
VERSION     DAWKOG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.255"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            79..600
                     /locus_tag="WJK66_002063"
     CDS             79..600
                     /locus_tag="WJK66_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579707.1"
                     /translation="MKQMLLAVGVAAVLAGCGKDAGSYEGYWREKSDKKEGMIVVKKE
                     KGNYFLNKINVVTGKEESMLLSEKDGELSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCRQTAQAYLDAQKALPSNQTYQQRQAAIEQLKRRFEAEFDELEK
                     EIKCNGRPRALLL"
BASE COUNT          225 a          156 c          213 g          158 t
ORIGIN      
        1 tttttgcaaa tcaatattga caagctgaaa aaaaaaaaca atatactcac ccagtcttaa
       61 tgttaacgga gtatggaaat gaaacaaatg cttttggccg tcggcgtggc ggcggtgttg
      121 gcgggctgcg gcaaggatgc cggtagttac gagggttatt ggcgcgaaaa atcggacaaa
      181 aaagagggta tgattgtcgt caaaaaagaa aaaggcaatt acttccttaa taaaatcaac
      241 gtggttacag gtaaggaaga atctatgctt ttgtctgaaa aagacggcga gctttcgata
      301 aacacgggga taggggaaat cccgatcaaa ctttccgacg acgggaaaga gctgtatgtc
      361 gaacgcaggc agtatgtcaa aaccgatgcg gcgatgaagg acaaaatcat cgcccaccag
      421 aaaaagtgca gacaaacggc acaggcatac ctcgacgcgc aaaaggcgtt gccgtcaaac
      481 caaacgtatc agcagcgtca ggcggcgatc gagcaattga aacggcggtt tgaagccgag
      541 ttcgatgaat tggaaaaaga aatcaaatgc aacggcagac cccgggcatt gttgctttag
      601 cggaggataa ccgggaggat gccgccgtcc gaatcggacg tgcggtttcc gtaccggtac
      661 gggcgggcag gaatgtccgc ctttttttgt tcggacgcgt ttgaataccc gtttgattcc
      721 gaccgtttgc aaggggtatt tccgttcggg cg
//
LOCUS       DAWKOG010000256          726 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.256, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000256 DAWKOG010000000
VERSION     DAWKOG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.256"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(129..>726)
                     /locus_tag="WJK66_002064"
     CDS             complement(129..>726)
                     /locus_tag="WJK66_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ7579708.1"
                     /translation="LSIGQIVKVKPAGYAAPKTAAVESRPAVPAAVQTPVKPAAQPPV
                     QSAPQPAAPAAENKAVPAPAPQSPAASPSGTRSVGGIVWQRPTQGKVVADFGGNNKGV
                     DIAGNAGQPVLAAADGKVVYAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQV
                     KRGQQVALMGNTDASRTQLHFEVRQNGKPVNPNSYIAF"
BASE COUNT          162 a          210 c          197 g          157 t
ORIGIN      
        1 ccaaacacag tcaaacaaat ttatatgccc catcccttcc gaataatttg aaaacacagc
       61 cgccaaaaac aaaaatgccg tctgaaaacc tttcagacgg catttccaac ttgatttcag
      121 gcagaaagtc agaacgcgat atagctgttc ggattaaccg gtttgccgtt ttgccgcacc
      181 tcgaaatgaa gctgcgttct ggaagcgtcg gtattgccca tcaaagcgac ctgctgcccg
      241 cgtttgacct gctggccttc gccgaccagc aatttttggt tgtgcccgta tgcggtcagg
      301 aaggaagaat tatgctggat gatgaccaga ttgccgtatc ccctcaaacc ggaacctgca
      361 taaaccactt tgccgtcagc cgccgccaaa acgggctgtc ccgcattacc ggcaatatcg
      421 acacccttgt tgttgccgcc gaaatcggca accactttac cttgcgtcgg acgctgccaa
      481 acaatgccgc cgaccgaacg cgtgccggaa ggcgaagcgg caggagattg cggggcgggc
      541 gcgggaaccg ctttattttc cgcagcgggc gcggcaggtt gcggcgcgga ctgcacaggc
      601 ggttgcgcgg cgggtttcac aggggtttgc acggcagccg gtacggcggg cctgctttct
      661 acggctgcgg ttttcggtgc ggcatatcct gccggtttga ctttaacaat ctgaccgatg
      721 ctcaac
//
LOCUS       DAWKOG010000257          708 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.257, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000257 DAWKOG010000000
VERSION     DAWKOG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.257"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(90..641)
                     /locus_tag="WJK66_002065"
     CDS             complement(90..641)
                     /locus_tag="WJK66_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579709.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKSGGAGKADG"
BASE COUNT          199 a          275 c          135 g           99 t
ORIGIN      
        1 caaagaaacc gccattagca ctacaccccc caagctgcgg ctgccaaaca catcggctgc
       61 ttctcgacgc acccgctgaa aaatgcgtcc tatccgtccg ccttgcccgc accccctgac
      121 tttgccgccc tgcccgatgc cgtctgaagc ctgaaccgcc ccaccagccc gatgacgcac
      181 gacaccgcag gaaagggcat acacaaagcc gtcagcacat aacgcgcatt ccccgtattg
      241 accacgtccg cgcccttcag cagcagcggc agcgcgttcg cccactccca caccgacggc
      301 gcgctgaacg ccaataccgc caccagcccc aacatccgga acaccccgat ttccaacacc
      361 tgccgccgcc gcaccgaacg gttgagcaaa aacacaaccg ccaacccgaa acagaccacc
      421 gccgccatac cgccgttggc gacaatctgc aaactgttga atgcaatatc gggcgtaacc
      481 gccaacacgc cttccgaatc cggcacggca cgctgcaaat ccaaaccctg caacacattc
      541 aacacaaaac cgtagatcaa atcaaaaaac accacgccta tcggcaaagc actcaaaacc
      601 cgccacatct tcctgtccga ccaaccgttc aaaactttca ttttacccga ccacgcaagc
      661 cgccgaacaa aaaataaggg gctgtcctag ataactagga taaactcg
//
LOCUS       DAWKOG010000258          703 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.258, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000258 DAWKOG010000000
VERSION     DAWKOG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.258"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     repeat_region   375..674
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=441..476
                     /rpt_unit_seq="gttgtagctccctttctcatttcgcagtgctacaat"
BASE COUNT          158 a          172 c          136 g          237 t
ORIGIN      
        1 tttgcaagat tttagtttaa tcatcaaaca gggaacagca aaaatccccg ctgcaacggg
       61 gatttttgtt cggctctcgc ctgtgtgatt aacttcacta ggagaagcta aaatgcttaa
      121 attccttatg cgtgggaaaa agttgctgct gtccgttgaa gtgaaccaca agttcatttt
      181 agcggttgct ttgctgttaa gtcaatgcag tagctaaccc gaccacaatc aaaaaaacgc
      241 aagttttccg cctactctgt gagtgggcgg ttttttctta tttcaaatct gtgacacaat
      301 atctgtcgcc tttcttgcca taattgcact gtgtctcaat gactttaacg cacgttcgct
      361 tatcgcaacg gctggttgta gcgcccattc tcatttcgca gtgctacaat tttttgtatc
      421 cattcccagc cggaaattaa gttgtagctc cctttctcat ttcgcagtgc tacaatcttt
      481 aactttgacc gtgtgcaatc cagttagttg tagctccctt tctcatttcg cagtgctaca
      541 atctgaccac acctatccgc cagtgcttat gcgttgtagc tccctttctc atttcgcagt
      601 gctacaataa gccggtttat ggcaattctg ccgcgtccgt tgtagctccc tttctcattt
      661 cgcagtgcta caatatgcct ttttttgtac tgttgtttga acg
//
LOCUS       DAWKOG010000259          694 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.259, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000259 DAWKOG010000000
VERSION     DAWKOG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.259"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(226..510)
                     /locus_tag="WJK66_002066"
     CDS             complement(226..510)
                     /locus_tag="WJK66_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ7579710.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMLVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWQDLWFDII"
BASE COUNT          201 a          181 c          121 g          191 t
ORIGIN      
        1 acgcggaatc tcaagaaacc gtttacccga taagtttccg tgccgacaga cctagattcc
       61 cgcctgcgcg ggaatgacgg gtcttttata atctttgaat attactgttg ttctaaggtc
      121 tagattcccg cctgcgcggg aatgacggtt tagaagttgc ccgaaacctc aaaaaaaaaa
      181 ccgaaaccga acaagccgga ttcccgcctg cgcgggaatg acgggctaaa taatatcaaa
      241 ccataaatcc tgccaagaaa cattattttc ttcaatcagt tgcaatttcc aagccctgtt
      301 ccatttcttc aactgttttt cccgagtaat tgcactctcc atcgtaggat gcagttcata
      361 ccaaaccagc atagtaacgt tgtaccgtga tgtaaatccc tcaatcaaat gctccctatg
      421 ttggtaaata cgttgcacca aatcagatgt aacgccaatg tataacgtgc cattacgttg
      481 gcttgctaaa atataaaccg caggctgcat ataataccct tttgaattat ttcaatttat
      541 attcccgcga acaccatccc gtgattactt taacccttcg ttattcccat agctttccat
      601 cattcccgca actcttcgtc attcccgcga aagtgggaat ctagaacgca aaatctaaag
      661 aaaccgtttt acccgataag tttccgcacc gaca
//
LOCUS       DAWKOG010000260          691 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.260, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000260 DAWKOG010000000
VERSION     DAWKOG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.260"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            231..530
                     /locus_tag="WJK66_002067"
     CDS             231..530
                     /locus_tag="WJK66_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ7579711.1"
                     /translation="MQRCFNWFDHYCRRLEKYFRRCSIMETWVQNYTAIGGSLYLTAA
                     AALLPIVFFFAALTVLKLKGYQAGLYTLLIALAVAVFGFGMPASMAVSSLPPQPH"
     gene            509..691
                     /locus_tag="WJK66_002068"
                     /pseudo
     CDS             509..691
                     /locus_tag="WJK66_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757319.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
BASE COUNT          183 a          197 c          144 g          167 t
ORIGIN      
        1 caaacatcaa attggactga ccaatcaggg cggattctaa tgacacgccg ttcccgccgt
       61 caacggcatt tacctcgcac cgcccccgaa acacaagaaa aaactacacc aaactacaat
      121 ttttgttcat gcaaatattt gttttgacag gatttaaacg aaaactccga ttcaaatctg
      181 ccgaaccgcc caaaaatata ttgacctaaa tattaaagtt tcgtaaagta atgcaacgtt
      241 gctttaattg gtttgaccac tattgccgac gattagaaaa atattttcgg agatgttcaa
      301 ttatggaaac ttgggttcaa aactacacgg caatcggcgg cagcctgtat ctgactgccg
      361 ccgccgcact cttacccatc gtctttttct ttgccgcgct gaccgtcctg aagctgaaag
      421 gctatcaggc ggggctttat acgctgctga ttgcgcttgc cgttgccgta ttcggcttcg
      481 ggatgccggc gagtatggcg gtttcttccc tgccgccgca gccgcactga cccaacgccc
      541 ctacgccacg ctggatttga ccgcgcatta caaaatcgga aaatccacac gcatcggttt
      601 ggactttgaa aacgtgttca acaaacgcta ccgcaccatg cccgacattc acgtttacgg
      661 cacgccgcgc agcctgaccg caaccgtcaa a
//
LOCUS       DAWKOG010000261          673 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.261, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000261 DAWKOG010000000
VERSION     DAWKOG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.261"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..289)
                     /locus_tag="WJK66_002069"
     CDS             complement(<1..289)
                     /locus_tag="WJK66_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579712.1"
                     /translation="MMYSFEANANAVKISETVSVDTGQGAKIHKFVPKNSKTYSSDLI
                     KTVDLTHIPTGAKARINAKITASISRAGVLAGGGKLARLGAKFSTRAVPYVG"
     gene            complement(273..590)
                     /locus_tag="WJK66_002070"
     CDS             complement(273..590)
                     /locus_tag="WJK66_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ7579713.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMAVIAQSEFVEFAKINKS
                     ENVIDCFSFFWNRRIKHDIWLISFSDNSEMVIKESLKDGHKIYKFEFCEIVDNCNFDD
                     VFV"
BASE COUNT          194 a          144 c          117 g          218 t
ORIGIN      
        1 ttccgacata gggaaccgcc cttgtgctga atttcgcgcc taagcgggca agtttgccgc
       61 cccccgccaa tacgccggcg cgggaaatgc tggcggttat tttggcgttg attcgggctt
      121 ttgcgcccgt agggatgtgt gttaaatcta ccgtttttat taaatcagat gaataagttt
      181 tactattttt aggtacaaac ttatgaattt tcgcaccttg tccggtatca actgaaacag
      241 tttcagatat ttttactgca tttgcattcg cttcaaacga atacatcatc aaaattgcaa
      301 ttatcgacaa tttcgcaaaa ttcaaatttg tatattttat gaccatcttt cagggattct
      361 ttaattacca tttctgaatt atcagaaaat gagattagcc aaatatcatg tttaattctt
      421 ctattccaga aaaaagagaa acaatcaata acattttcag acttattaat cttcgcaaat
      481 tcaacaaatt cagattgcgc tataaccgcc atcgattgcc caaaatactt gctagacggc
      541 tgatatttat aaagtgccaa ctgcgcctga gtgataaacg gcttgttcat ggttctgcct
      601 ttcaatgatt gttttgaaag cctgattttg acaccataac ttcatgcgct caattcttaa
      661 acagaaccgc ccc
//
LOCUS       DAWKOG010000262          657 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.262, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000262 DAWKOG010000000
VERSION     DAWKOG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.262"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
BASE COUNT          175 a          190 c          144 g          148 t
ORIGIN      
        1 tccgcacaga aacttatcgg ataaaacggt ttctttagat tttacgtcct agattcccac
       61 ttccgtggga atgacggttc agttgcattc ccgacaacaa cgcaatctcg aaacccgtca
      121 ttcctgcgca ggcgggaatc cagacctccg acgcggcggg aatctatcgg aaatgactga
      181 aacccccgag attctagatt cccacttccg tgggaatgac ggttcagttg cgttccgaca
      241 acaccgcaat ctcgaaaccc gtcattcccg cgcaggcggg aatctagacc cccgacgcgg
      301 cgggaatcta tcggaaatga ctgaaacccc gagattctag attcccactt tcgtgggaat
      361 gacgattcag tttcgttccg acaacaccgt aatcttgaaa cccgtcattc ccgcgcaggc
      421 gggaatccag ccccctgacg cggcgggaat ctatcggaaa tgactgaaac cccgcgttct
      481 agattcccac ttccgtggga atgacggttc agcaagcgta ggtcggatac ttgtatccga
      541 caaaaccttt aacattccca tcattgcaat ccattgcagc aatgcccaaa atgtcgaatt
      601 caagaatccg acctacaaaa tcattccgag cataacatta tgaaataccg tcgttgt
//
LOCUS       DAWKOG010000263          652 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.263, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000263 DAWKOG010000000
VERSION     DAWKOG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.263"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(51..536)
                     /gene="fxsA"
                     /locus_tag="WJK66_002071"
     CDS             complement(51..536)
                     /gene="fxsA"
                     /locus_tag="WJK66_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HEZ7579714.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAAGFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
BASE COUNT          195 a          211 c          116 g          130 t
ORIGIN      
        1 tcaaaccgga aaaatgccgt ctgaatcttc agacggcatt ccattcatat ttattcgtct
       61 tttttgtgtt cgatggcgtt tcgggaacga ttgccgccgt aaggctcttc aaccgtatat
      121 tccccctcga taatatcgtc atcgcgggaa aagccctctt ttctgcccga ttggttcatg
      181 ttgaaaaaat tttccgcacc tcctgcctgc aacactgcac ctcccttaaa cggcagcagc
      241 agcaataccg ccaacaccga ggatacgaat cccggactca tcagacacac cgccgccacc
      301 gtataacgga taggccacaa catctgataa acggataccc tcccgccgct tctcattgcc
      361 gcgcccgcca ataaaagacc ggacagcccc gtatgcctga gcatcagcac accggcggca
      421 aaacctgccg ccatcaaaaa caacgtccag ccgccgccca accaatcggc aacccacaca
      481 atcgacataa tctccaaaaa cagcagcacc aaaaaaccga taccgaaaaa tctcattgac
      541 cgtcatcctt atatttaagt aaacagcaaa ccgcccgaac aagactccaa gcgacctgcc
      601 tgtaaatgat tacaaaacca tgtgcttcaa gccgaaacaa tgtgaaatca cg
//
LOCUS       DAWKOG010000264          609 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.264, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000264 DAWKOG010000000
VERSION     DAWKOG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.264"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            208..501
                     /locus_tag="WJK66_002072"
     CDS             208..501
                     /locus_tag="WJK66_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579715.1"
                     /translation="MKTNDKLNRQIDVLQKQSAAIHNEAYIEMNTLLYRHREVVSVHN
                     RKADYAEKGKEQIALFPRGLNGITKLPAAVLLPERPYHFDMKEVLHIFSWIPR"
BASE COUNT          159 a          157 c          152 g          141 t
ORIGIN      
        1 tcgccttgtc ctgatttttg ttaatccact atattgtcca ttcggcaggg aaagcctgaa
       61 gaagaatttt cccgcaagat ggccggctgc ctaaccgact tggaaaactg tttggaaaaa
      121 tacctcgaac agttcgggcc ggtatcggaa agtatcgaag cctgtaccgt caaactgcag
      181 gagtaaccgt cgttcttcaa caggctgatg aaaaccaacg acaagctcaa taggcagatt
      241 gacgtgttgc agaaacagtc ggcagccatc cacaacgaag cctatatcga aatgaacacg
      301 ctgctttacc gccaccgcga agtggtttcc gtccacaacc gcaaagcaga ttatgcggaa
      361 aagggcaaag agcagattgc gctgttcccg cgcggattga acggcatcac caagctgccc
      421 gccgccgtcc tgttgcccga gcgtccctac cattttgata tgaaggaggt tctgcatata
      481 ttctcctgga taccgaggta ggcgtatcgg ctgccggata aaaatgcacc ccgtcattcc
      541 tgtctgtacg ggggtagcgg cagtgttttc ctgcttcgtt ttatagtgga ttaacaaaaa
      601 ccagtacgg
//
LOCUS       DAWKOG010000265          582 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.265, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000265 DAWKOG010000000
VERSION     DAWKOG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.265"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            32..394
                     /locus_tag="WJK66_002073"
     CDS             32..394
                     /locus_tag="WJK66_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEZ7579716.1"
                     /translation="MNQLDQLGTRINLICNVFDKWIGQQDLNYNLFAVLYTLATEGSR
                     TQKHIGEKWSLPKQTVSGVCKTLAGQGLIEWQESEQDRRERLLSLTEKGKAHAAPLTE
                     SAQEFSDKETFAKFPKIP"
     gene            425..>582
                     /locus_tag="WJK66_002074"
     CDS             425..>582
                     /locus_tag="WJK66_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ7579717.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YLRDHRGRP"
BASE COUNT          179 a          162 c          123 g          118 t
ORIGIN      
        1 cgcacaaatt tagtcaatat caagaccaat tatgaaccaa ctcgaccaac ttggcacccg
       61 tatcaacctg atttgcaatg tcttcgacaa atggatcggg cagcaggatc tgaattacaa
      121 cctctttgcc gtactttata ccctggcaac cgaaggcagc cgcacgcaaa agcatatcgg
      181 cgaaaagtgg agtctgccca aacagaccgt ttcaggcgta tgcaaaaccc ttgccggaca
      241 agggttgatt gaatggcagg aaagcgaaca ggaccggcgc gaacggttgc tgtcgctgac
      301 cgaaaaaggc aaagcccatg ccgcaccttt aacagaaagc gcgcaggaat tcagcgacaa
      361 agagaccttt gcaaaattcc ccaaaatccc ctaaattccc accaagacat ttaggagatt
      421 ttccatgagc accttctttc aacaaaccgc gcaagccatg attgctaaac acatcgaccg
      481 tttccctcta ttgaagttgg atcaagtgat tgattggcag ccgatcgaac aatacctgaa
      541 tcgtcaaaaa acccgttacc ttcgagacca ccgcggccgt cc
//
LOCUS       DAWKOG010000266          574 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.266, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000266 DAWKOG010000000
VERSION     DAWKOG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.266"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..540
                     /locus_tag="WJK66_002075"
     CDS             <1..540
                     /locus_tag="WJK66_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ7579718.1"
                     /translation="QAIEVDEEGQVSGQTTPSKDSDARWTKKNGLYKLGYKQHTRTDA
                     EGYIEKLHITPANAHECKHLSPLLEGIAEGTTVYADKGYDSAENRQHLKEHQLQDGIM
                     RKACRNRPLSEAQTKRNRYLSKTRYVVEQSFGTLHRKFRYARAAYFGLIKVSAQSHLK
                     AMCLNLLKAPNRLSAPVAA"
BASE COUNT          167 a          155 c          149 g          103 t
ORIGIN      
        1 caggccatag aagttgacga agaaggacaa gtcagcggcc aaaccacacc gagtaaagac
       61 agcgatgccc gttggacaaa gaaaaacggc ctctacaaac tcggttacaa acagcatacc
      121 cgtaccgatg cagaaggcta tatcgagaaa ctgcacatta cccccgccaa tgcccatgag
      181 tgcaaacacc tgtcgccttt gctggaaggg atagccgaag gtacgaccgt ctatgccgac
      241 aaaggctatg acagtgcgga aaaccggcaa catctgaaag aacatcagtt gcaggacggc
      301 attatgcgca aagcctgccg caaccgtccg ctgtcggaag cgcaaaccaa acgcaaccgg
      361 tatttgtcga agacccgtta tgtggttgaa caaagcttcg gtacgctgca ccgtaagttc
      421 cgctatgccc gggcagccta tttcggactg attaaagtga gtgcgcaaag ccacctgaag
      481 gcgatgtgtt tgaacctgtt gaaagcgccc aacaggttaa gtgcgcctgt tgccgcctaa
      541 aaagcagccc ggatgcctga ttatcgggta tccg
//
LOCUS       DAWKOG010000267          569 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.267, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000267 DAWKOG010000000
VERSION     DAWKOG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.267"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            1..>569
                     /locus_tag="WJK66_002076"
     CDS             1..>569
                     /locus_tag="WJK66_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ7579719.1"
                     /translation="MHYIDIAKRNFVIAVSSLSKTKTETNNPKGIAHTIEYLKKHKVA
                     LVVTESTGGLEIPAAKAIRRAGIAVIIANPRQTHQFAQSQPLTKTDAKDAKMPAFFAQ
                     MTAQKEDSQTMPYQPPTEAEEVLEALVNRRNQSADMRTAEKNRLHQVHETQVGSVKQL
                     IAHFDRLIDELDKQIDNHTHTHFDGKAQVA"
BASE COUNT          174 a          179 c          120 g           96 t
ORIGIN      
        1 attcactata tcgacatcgc caaacgaaac ttcgtcatcg ccgtttcgtc tttgtctaaa
       61 accaaaaccg aaaccaacaa ccccaaaggt atcgcccata ctatcgaata ccttaaaaaa
      121 cacaaggtcg ccctcgtcgt gacggaaagt actggcggtc tcgaaatccc cgccgccaaa
      181 gccatccgcc gagcaggcat agccgtgatt atcgccaacc cgcgtcagac gcatcagttt
      241 gcccaatcgc agccgctgac caaaaccgac gccaaagatg ccaaaatgcc cgccttcttc
      301 gcacagatga cggcacagaa agaagattcg caaaccatgc cctaccaacc gcccaccgaa
      361 gcggaagaag tgttggaagc cttggttaac cgccgcaacc aatcggcgga tatgcggact
      421 gccgagaaaa accgtctgca tcaggttcac gaaacgcaag tcggaagcgt caaacaactg
      481 attgcccatt ttgaccggct gattgacgaa ttggacaaac aaatcgacaa ccacacccac
      541 acgcattttg acggcaaagc ccaagtggc
//
LOCUS       DAWKOG010000268          544 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.268, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000268 DAWKOG010000000
VERSION     DAWKOG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.268"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            58..345
                     /locus_tag="WJK66_002077"
     CDS             58..345
                     /locus_tag="WJK66_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579720.1"
                     /translation="MDDLIAAFLGCLSGKYSEGKISRLEISLTAGSIFFAVFFIHELI
                     IFIVDGNKENLWSFITGIMKDLFLFSCSVCLLFYFLLFIAETIGKTINKIK"
BASE COUNT          181 a           64 c           92 g          207 t
ORIGIN      
        1 aatggcaaaa caggtaatga attaaagaca ttacctgttt ttacagaaat ttttaaaatg
       61 gatgatttga ttgcagcttt tttaggctgt ttatcaggta aatatagcga agggaaaata
      121 agcagattag aaatatctct aacggcagga agcattttct ttgcagtatt ctttattcat
      181 gaactgataa tttttattgt tgatggaaat aaggaaaatc tttggagttt tattactggg
      241 ataatgaaag atttattttt attttcatgt tctgtttgtt tattattcta cttcttatta
      301 tttattgcgg aaaccattgg gaaaacaatc aataagatta aataagatat ttaagccgct
      361 aaaaaaccat ttgagtattt tatcttctat gcggcattta tcttggaatt gaaatactgc
      421 aatcgggagg ctaaggcttc cgttgtaacg tttaccatat atttcattat tttaatttca
      481 tcaaaaaaag aaattcaaga ttatacatac cgccaatagt ttcttgattg ttttgtatag
      541 tgga
//
LOCUS       DAWKOG010000269          519 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.269, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000269 DAWKOG010000000
VERSION     DAWKOG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.269"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            110..>519
                     /locus_tag="WJK66_002078"
     CDS             110..>519
                     /locus_tag="WJK66_002078"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ7579721.1"
                     /translation="MDETGFDRYLFRPYARSLKGQIVKAQISGKRYQRLSLVSAQVGN
                     RLIAPMVYQNTMTGVFFEAWFQQCLLPALTQKSVIILDNARFHRMGVLREMAEKLGHK
                     VLPLAPYSPELNPIEKVWANIKRYLRTVLSDYAR"
BASE COUNT          147 a          118 c          123 g          131 t
ORIGIN      
        1 tggggataac gcgcaaaaaa agaccaccac ttacaaagaa caagacccgg ccaaagtaac
       61 gcattatttg acacagccgg ccgaattttc cgactaccaa cgtgtttatt tggatgaaac
      121 aggatttgac cgctacctgt tccgtcccta tgcccgcagc ctgaaagggc aaatagtgaa
      181 agcgcagata agtggaaaaa gataccaacg cttatctttg gtgtccgcac aagtcggcaa
      241 ccggctgatt gctccgatgg tttatcaaaa tacgatgacc ggagtctttt ttgaagcgtg
      301 gtttcagcaa tgcctactgc ccgcattgac tcaaaaatcg gtgattattt tagataatgc
      361 gcgatttcac cgtatgggtg tcttacggga aatggcggaa aaattgggac ataaggtatt
      421 gcctcttgca ccttattcac ctgagctcaa cccgattgag aaagtgtggg cgaatattaa
      481 gcggtatctg cgaaccgttt tgtctgatta cgcccgatt
//
LOCUS       DAWKOG010000270          517 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22431_Neisseria
            meningitidis SAMN10275863-rid8981473.denovo.270, whole genome
            shotgun sequence.
ACCESSION   DAWKOG010000270 DAWKOG010000000
VERSION     DAWKOG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275863
            Sequence Read Archive: SRR8200334
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676322.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676322.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:43:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,028
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 47 of 88
            Pseudo Genes (incomplete)         :: 38 of 88
            Pseudo Genes (internal stop)      :: 10 of 88
            Pseudo Genes (multiple problems)  :: 7 of 88
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275863-rid8981473.denovo.270"
                     /isolate="M22431_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="1978"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(76..246)
                     /locus_tag="WJK66_002079"
     CDS             complement(76..246)
                     /locus_tag="WJK66_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEZ7579722.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            259..477
                     /locus_tag="WJK66_002080"
     CDS             259..477
                     /locus_tag="WJK66_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7579723.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
BASE COUNT          134 a          105 c          137 g          141 t
ORIGIN      
        1 gggcaatcac tcccgcaaag tcggaaactg acaaaaaagc ccgcgtaggg cgcgggctga
       61 gggtgaaagc ggattttata cctcttttac aggggtagcg gcggtagtgc ttttcagcaa
      121 atcgactgcg tgctggcagt tttgcttgct ggtgtagcct tcgccctgag cgatgatttc
      181 atggttggct gctttcaaac gccaacggta ttcgcctttt gcgtctttat agatttcaaa
      241 atacataagg tttctcctat gaatgagtac acgttttctt accgctttaa cggcaagtcc
      301 tggtcattga gcatttgggc ggacaaccct gaagaagcca gggcgaaatt tcgggctgca
      361 cgagaaaatg cgcactatga cggcgaagtt gtagcaaagg tttatacatt tgtaaatatt
      421 tcgtgggtta agaaattgta caagcggaca aaatatttaa tgggtatcaa agaatgacct
      481 accgtgaatt agttgaacgt cagttggctg tgcgcca
//
